LOCUS       DAXFWM010000001        85668 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000001 DAXFWM010000000
VERSION     DAXFWM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 85668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85668
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.001"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(121..303)
                     /locus_tag="ACFI8B_000001"
     CDS             complement(121..303)
                     /locus_tag="ACFI8B_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833342.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            303..638
                     /locus_tag="ACFI8B_000002"
     CDS             303..638
                     /locus_tag="ACFI8B_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833343.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(675..1334)
                     /gene="ung"
                     /locus_tag="ACFI8B_000003"
     CDS             complement(675..1334)
                     /gene="ung"
                     /locus_tag="ACFI8B_000003"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC3833344.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            1415..1822
                     /locus_tag="ACFI8B_000004"
     CDS             1415..1822
                     /locus_tag="ACFI8B_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC3833345.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            1837..2784
                     /locus_tag="ACFI8B_000005"
     CDS             1837..2784
                     /locus_tag="ACFI8B_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC3833346.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(2891..3961)
                     /locus_tag="ACFI8B_000006"
     CDS             complement(2891..3961)
                     /locus_tag="ACFI8B_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC3833347.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            4146..4421
                     /locus_tag="ACFI8B_000007"
     CDS             4146..4421
                     /locus_tag="ACFI8B_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC3833348.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            4423..5046
                     /gene="lipB"
                     /locus_tag="ACFI8B_000008"
     CDS             4423..5046
                     /gene="lipB"
                     /locus_tag="ACFI8B_000008"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC3833349.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            5039..6022
                     /gene="lipA"
                     /locus_tag="ACFI8B_000009"
     CDS             5039..6022
                     /gene="lipA"
                     /locus_tag="ACFI8B_000009"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC3833350.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            6498..6962
                     /gene="bfr"
                     /locus_tag="ACFI8B_000010"
     CDS             6498..6962
                     /gene="bfr"
                     /locus_tag="ACFI8B_000010"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC3833351.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARSELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            6990..7463
                     /gene="bfr"
                     /locus_tag="ACFI8B_000011"
     CDS             6990..7463
                     /gene="bfr"
                     /locus_tag="ACFI8B_000011"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC3833352.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            7791..8111
                     /locus_tag="ACFI8B_000012"
     CDS             7791..8111
                     /locus_tag="ACFI8B_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3833353.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            8160..10718
                     /gene="glnD"
                     /locus_tag="ACFI8B_000013"
     CDS             8160..10718
                     /gene="glnD"
                     /locus_tag="ACFI8B_000013"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC3833354.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPVPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            10865..12328
                     /gene="guaB"
                     /locus_tag="ACFI8B_000014"
     CDS             10865..12328
                     /gene="guaB"
                     /locus_tag="ACFI8B_000014"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC3833355.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVTVGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(12664..15039)
                     /gene="rnr"
                     /locus_tag="ACFI8B_000015"
     CDS             complement(12664..15039)
                     /gene="rnr"
                     /locus_tag="ACFI8B_000015"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC3833356.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPEFGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKGKS"
     gene            15128..15214
                     /locus_tag="ACFI8B_000016"
     tRNA            15128..15214
                     /locus_tag="ACFI8B_000016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15162..15164,aa:Leu,seq:cag)
     gene            15253..15343
                     /locus_tag="ACFI8B_000017"
     tRNA            15253..15343
                     /locus_tag="ACFI8B_000017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15287..15289,aa:Ser,seq:gga)
     gene            complement(15740..17551)
                     /gene="typA"
                     /locus_tag="ACFI8B_000018"
     CDS             complement(15740..17551)
                     /gene="typA"
                     /locus_tag="ACFI8B_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC3833357.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(17668..18558)
                     /locus_tag="ACFI8B_000019"
     CDS             complement(17668..18558)
                     /locus_tag="ACFI8B_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC3833358.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            18623..19279
                     /locus_tag="ACFI8B_000020"
     CDS             18623..19279
                     /locus_tag="ACFI8B_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC3833359.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            19708..20790
                     /locus_tag="ACFI8B_000021"
     CDS             19708..20790
                     /locus_tag="ACFI8B_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC3833360.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
     gene            20787..22580
                     /locus_tag="ACFI8B_000022"
                     /pseudo
     CDS             20787..22580
                     /locus_tag="ACFI8B_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            22607..24372
                     /gene="cysI"
                     /locus_tag="ACFI8B_000023"
                     /pseudo
     CDS             22607..24372
                     /gene="cysI"
                     /locus_tag="ACFI8B_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            24545..26404
                     /gene="ilvD"
                     /locus_tag="ACFI8B_000024"
     CDS             24545..26404
                     /gene="ilvD"
                     /locus_tag="ACFI8B_000024"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC3833361.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(26664..26900)
                     /locus_tag="ACFI8B_000025"
     CDS             complement(26664..26900)
                     /locus_tag="ACFI8B_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833362.1"
                     /translation="MVCTFFRRTAFVKQRTFKVADGNVRIFQDGFDVFQDIGIGRVID
                     RIIAAEHNAADPDDGNGICCLSVRRILSVRIAGV"
     gene            26890..27663
                     /locus_tag="ACFI8B_000026"
     CDS             26890..27663
                     /locus_tag="ACFI8B_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC3833363.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(27759..28811)
                     /gene="bioB"
                     /locus_tag="ACFI8B_000027"
     CDS             complement(27759..28811)
                     /gene="bioB"
                     /locus_tag="ACFI8B_000027"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC3833364.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSVIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(29018..30394)
                     /gene="mpl"
                     /locus_tag="ACFI8B_000028"
     CDS             complement(29018..30394)
                     /gene="mpl"
                     /locus_tag="ACFI8B_000028"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC3833365.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            complement(30456..30761)
                     /locus_tag="ACFI8B_000029"
     CDS             complement(30456..30761)
                     /locus_tag="ACFI8B_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3833366.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKDCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            complement(30773..31126)
                     /locus_tag="ACFI8B_000030"
     CDS             complement(30773..31126)
                     /locus_tag="ACFI8B_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833367.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEEQ"
     gene            complement(31388..31585)
                     /locus_tag="ACFI8B_000031"
     CDS             complement(31388..31585)
                     /locus_tag="ACFI8B_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833368.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            complement(31595..32380)
                     /locus_tag="ACFI8B_000032"
     CDS             complement(31595..32380)
                     /locus_tag="ACFI8B_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC3833369.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADEILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            complement(32454..33764)
                     /gene="tilS"
                     /locus_tag="ACFI8B_000033"
     CDS             complement(32454..33764)
                     /gene="tilS"
                     /locus_tag="ACFI8B_000033"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC3833370.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            complement(33838..34791)
                     /locus_tag="ACFI8B_000034"
     CDS             complement(33838..34791)
                     /locus_tag="ACFI8B_000034"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC3833371.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFS"
     gene            35003..35404
                     /locus_tag="ACFI8B_000035"
     CDS             35003..35404
                     /locus_tag="ACFI8B_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC3833372.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            complement(35472..35669)
                     /gene="iscX"
                     /locus_tag="ACFI8B_000036"
     CDS             complement(35472..35669)
                     /gene="iscX"
                     /locus_tag="ACFI8B_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC3833373.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            35882..36422
                     /locus_tag="ACFI8B_000037"
                     /pseudo
     CDS             35882..36422
                     /locus_tag="ACFI8B_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(36482..36823)
                     /gene="fdx"
                     /locus_tag="ACFI8B_000038"
     CDS             complement(36482..36823)
                     /gene="fdx"
                     /locus_tag="ACFI8B_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC3833374.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(36879..37631)
                     /locus_tag="ACFI8B_000039"
     CDS             complement(36879..37631)
                     /locus_tag="ACFI8B_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC3833375.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLINVEQGESVCVPVG
                     DISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEKEHP
                     ENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLEYGA
                     NPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            complement(37667..38315)
                     /locus_tag="ACFI8B_000040"
                     /pseudo
     CDS             complement(37667..38315)
                     /locus_tag="ACFI8B_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(38501..40363)
                     /gene="hscA"
                     /locus_tag="ACFI8B_000041"
     CDS             complement(38501..40363)
                     /gene="hscA"
                     /locus_tag="ACFI8B_000041"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC3833376.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            40534..41406
                     /gene="hpnD"
                     /locus_tag="ACFI8B_000042"
     CDS             40534..41406
                     /gene="hpnD"
                     /locus_tag="ACFI8B_000042"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC3833377.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            41481..42794
                     /gene="hpnE"
                     /locus_tag="ACFI8B_000043"
     CDS             41481..42794
                     /gene="hpnE"
                     /locus_tag="ACFI8B_000043"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC3833378.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            42876..43595
                     /locus_tag="ACFI8B_000044"
     CDS             42876..43595
                     /locus_tag="ACFI8B_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3833379.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAGSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            43761..44432
                     /locus_tag="ACFI8B_000045"
     CDS             43761..44432
                     /locus_tag="ACFI8B_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC3833380.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            44442..44809
                     /locus_tag="ACFI8B_000046"
                     /pseudo
     CDS             44442..44809
                     /locus_tag="ACFI8B_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            44806..45453
                     /locus_tag="ACFI8B_000047"
     CDS             44806..45453
                     /locus_tag="ACFI8B_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC3833381.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            45846..47612
                     /locus_tag="ACFI8B_000048"
                     /pseudo
     CDS             45846..47612
                     /locus_tag="ACFI8B_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            47716..48384
                     /gene="hda"
                     /locus_tag="ACFI8B_000049"
     CDS             47716..48384
                     /gene="hda"
                     /locus_tag="ACFI8B_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC3833382.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            48381..49049
                     /locus_tag="ACFI8B_000050"
     CDS             48381..49049
                     /locus_tag="ACFI8B_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC3833383.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            49054..49341
                     /locus_tag="ACFI8B_000051"
     CDS             49054..49341
                     /locus_tag="ACFI8B_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating enzyme"
                     /protein_id="HFC3833384.1"
                     /translation="MPSEARFGVQTALRLKSQKPKATGIHPEKQKTQEFIGKNNNLST
                     VIPTKVGILIRPHGNLYFVIPPIPHHPYRFKSAQKSTFRHSHESGNLGRKI"
     gene            complement(49631..50527)
                     /gene="argB"
                     /locus_tag="ACFI8B_000052"
     CDS             complement(49631..50527)
                     /gene="argB"
                     /locus_tag="ACFI8B_000052"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC3833385.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARNVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGKKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            50697..51827
                     /locus_tag="ACFI8B_000053"
     CDS             50697..51827
                     /locus_tag="ACFI8B_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC3833386.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            51901..52752
                     /gene="lgt"
                     /locus_tag="ACFI8B_000054"
     CDS             51901..52752
                     /gene="lgt"
                     /locus_tag="ACFI8B_000054"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC3833387.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(52837..53499)
                     /locus_tag="ACFI8B_000055"
     CDS             complement(52837..53499)
                     /locus_tag="ACFI8B_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC3833388.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            53533..53639
                     /locus_tag="ACFI8B_000056"
                     /pseudo
     CDS             53533..53639
                     /locus_tag="ACFI8B_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(53698..55491)
                     /locus_tag="ACFI8B_000057"
     CDS             complement(53698..55491)
                     /locus_tag="ACFI8B_000057"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC3833389.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            55737..56846
                     /gene="proB"
                     /locus_tag="ACFI8B_000058"
     CDS             55737..56846
                     /gene="proB"
                     /locus_tag="ACFI8B_000058"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC3833390.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            56859..58121
                     /locus_tag="ACFI8B_000059"
     CDS             56859..58121
                     /locus_tag="ACFI8B_000059"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC3833391.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEVAAEDI
                     LAANRLDLKAAAGKIPDSMIDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLER
                     LLVRGRIEAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEARGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(58297..61341)
                     /locus_tag="ACFI8B_000060"
     CDS             complement(58297..61341)
                     /locus_tag="ACFI8B_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC3833392.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAVPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            61506..62186
                     /locus_tag="ACFI8B_000061"
     CDS             61506..62186
                     /locus_tag="ACFI8B_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833393.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTAALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            62215..62574
                     /locus_tag="ACFI8B_000062"
     CDS             62215..62574
                     /locus_tag="ACFI8B_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC3833394.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            62652..62758
                     /locus_tag="ACFI8B_000063"
                     /pseudo
     CDS             62652..62758
                     /locus_tag="ACFI8B_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(63179..63715)
                     /locus_tag="ACFI8B_000064"
     CDS             complement(63179..63715)
                     /locus_tag="ACFI8B_000064"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC3833395.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(63749..64105)
                     /gene="folB"
                     /locus_tag="ACFI8B_000065"
     CDS             complement(63749..64105)
                     /gene="folB"
                     /locus_tag="ACFI8B_000065"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC3833396.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            64161..64763
                     /gene="plsY"
                     /locus_tag="ACFI8B_000066"
     CDS             64161..64763
                     /gene="plsY"
                     /locus_tag="ACFI8B_000066"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC3833397.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHPSWIFATLAIAILVLLRHKSNILNLIKGKEGKIGEKR"
     gene            64855..65700
                     /locus_tag="ACFI8B_000067"
     CDS             64855..65700
                     /locus_tag="ACFI8B_000067"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC3833398.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPADFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESHKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            complement(65808..66140)
                     /locus_tag="ACFI8B_000068"
     CDS             complement(65808..66140)
                     /locus_tag="ACFI8B_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HFC3833399.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(66190..66438)
                     /locus_tag="ACFI8B_000069"
     CDS             complement(66190..66438)
                     /locus_tag="ACFI8B_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833400.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            complement(66495..66656)
                     /locus_tag="ACFI8B_000070"
     CDS             complement(66495..66656)
                     /locus_tag="ACFI8B_000070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833401.1"
                     /translation="MPSERRQIAAGRSFGAFRRISRGIVVEKNGAGQVGTRQADREVQ
                     DAKFIFKKY"
     gene            66681..67655
                     /locus_tag="ACFI8B_000071"
     CDS             66681..67655
                     /locus_tag="ACFI8B_000071"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC3833402.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LAFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(67814..68032)
                     /locus_tag="ACFI8B_000072"
     CDS             complement(67814..68032)
                     /locus_tag="ACFI8B_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC3833403.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            68226..68489
                     /locus_tag="ACFI8B_000073"
     CDS             68226..68489
                     /locus_tag="ACFI8B_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC3833404.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIDRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            68663..70431
                     /gene="ggt"
                     /locus_tag="ACFI8B_000074"
                     /pseudo
     CDS             68663..70431
                     /gene="ggt"
                     /locus_tag="ACFI8B_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            70495..70659
                     /locus_tag="ACFI8B_000075"
     CDS             70495..70659
                     /locus_tag="ACFI8B_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833405.1"
                     /translation="MSNQTKIQAIKQASEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            70604..70846
                     /locus_tag="ACFI8B_000076"
     CDS             70604..70846
                     /locus_tag="ACFI8B_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833406.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            70913..72163
                     /gene="glyA"
                     /locus_tag="ACFI8B_000077"
     CDS             70913..72163
                     /gene="glyA"
                     /locus_tag="ACFI8B_000077"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC3833407.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVCGQVTALCDKYPVYGN"
     gene            complement(72236..72625)
                     /locus_tag="ACFI8B_000078"
     CDS             complement(72236..72625)
                     /locus_tag="ACFI8B_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3833408.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            73565..74356
                     /locus_tag="ACFI8B_000079"
     CDS             73565..74356
                     /locus_tag="ACFI8B_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFC3833409.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGVSADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            74507..75151
                     /locus_tag="ACFI8B_000080"
     CDS             74507..75151
                     /locus_tag="ACFI8B_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC3833410.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            75170..77080
                     /locus_tag="ACFI8B_000081"
     CDS             75170..77080
                     /locus_tag="ACFI8B_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3833411.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            77102..77468
                     /locus_tag="ACFI8B_000082"
                     /pseudo
     CDS             77102..77468
                     /locus_tag="ACFI8B_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            77580..79055
                     /gene="trpE"
                     /locus_tag="ACFI8B_000083"
     CDS             77580..79055
                     /gene="trpE"
                     /locus_tag="ACFI8B_000083"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC3833412.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            79139..80077
                     /locus_tag="ACFI8B_000084"
     CDS             79139..80077
                     /locus_tag="ACFI8B_000084"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3833413.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            80078..80938
                     /locus_tag="ACFI8B_000085"
     CDS             80078..80938
                     /locus_tag="ACFI8B_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HFC3833414.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            80955..82091
                     /locus_tag="ACFI8B_000086"
     CDS             80955..82091
                     /locus_tag="ACFI8B_000086"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC3833415.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            82138..82620
                     /locus_tag="ACFI8B_000087"
     CDS             82138..82620
                     /locus_tag="ACFI8B_000087"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3833416.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            82726..83774
                     /locus_tag="ACFI8B_000088"
                     /pseudo
     CDS             82726..83774
                     /locus_tag="ACFI8B_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            84354..84959
                     /locus_tag="ACFI8B_000089"
     CDS             84354..84959
                     /locus_tag="ACFI8B_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC3833417.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKIPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            85022..85384
                     /gene="ssrA"
                     /locus_tag="ACFI8B_000090"
     tmRNA           85022..85384
                     /gene="ssrA"
                     /locus_tag="ACFI8B_000090"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT        21018 a        24359 c        21015 g        19276 t
ORIGIN      
        1 atggattgaa tagatatttt tacaatctca ttcaaaactc agacacttaa aaaatttaat
       61 cagaacaaat aaaaaagcaa acaaaaatat ccatctttca aactcatatt ttgcaataac
      121 ctagataagc aagaacaggg gcagaacgga ttcgcctttg atgtggtggt aggcaaccat
      181 tccaatatgt cccagcgaca aaaacagcaa tgaaaatgcc agcgtgccgt gaattcctcc
      241 gccattgccg atttgacggg caatgcctgc ggcggaacag cgagcatcag ggcatacgcc
      301 caatgcccaa tttggtgacg gcggtgttgg cggggcggtt tttacgttgg cggattccct
      361 accctacaca ccaaacagac aatatcagca gcaggattct gactgcacgg atatgttggt
      421 atgaatcgtc cgcccaagcg gaaaaggctg tcgcgcccaa aacagtggca agaataaatt
      481 gcttgaattt catgatgttg cttcttttgc acaaagcgtt cggaatagtg aagcccgcag
      541 cctatggctt tgcggcagtc gcagctttac aagcggtatt gcaggaaacc ttaaaacacg
      601 atcattgcat caagagctgt tcgaattccg ccaaatgaac ctgacacgca aactttgacg
      661 cttgaatacg gcattcatag cttccagttt atcggttcga taccgtgttg actcaaatag
      721 ctgtttgcct gtgaaaaatg gcggcagcca aaaaaaccgc gatatgccga cagaggagac
      781 ggatgaggcg cggtcagtat caaatgattt tgactgtcta tcagcctccc tttttgttgt
      841 gcatacccac cccacaacat gaagacaagg tgcttgcggt gtgtcgcaag ctgcctgatg
      901 acggtatcgg taaagcgttc ccagcctaaa agggcgtgcg aatgcgcctg tcctgcacgc
      961 accgtcaaaa ccgtgttcag aagcaatatg ccctgctccg cccacgctgt caggcagccg
     1021 tgcgcgggaa tggaaaagcc ttcgatgtcg gtttccaatt ctttgtaaat attgagtaaa
     1081 gacggcggta tgcggacacc ctgccggacg gaaaatgcca aaccgtgcgc ctgccctacc
     1141 ccgtgatacg gatcttgtcc gagaatgacg actttgaccc ggtcgaacgc tgtcagccgg
     1201 aatgcgttga acacatcggc ctccggcgga tagataattt gccccgacaa acgttcctgc
     1261 ctgaccgcat ttaaaatttc ctgaaaatac ggctgctgtt tttcgccgcc gagtgcatcg
     1321 tgccaagtgt ccatatctgc cctatccttt caaattcggc tatactgctg atattgcgtt
     1381 attttgtcaa acgacgcgct ttgtggggaa atgtatgact gtatggtttg ttgccgctgt
     1441 tgccgtctta atcatcgaat tattgacggg aacggtttat cttttggttg tcagcgcggc
     1501 tttggcgggt tcgggcattg cctacgggct gactggcagc acgcctgccg ccgtcttgac
     1561 cgccgcactg ctttccgcgc tgggcatttg gttcgtacat gccaaaaccg ccgtgggaaa
     1621 agttgaaacg gattcatatc aggatttgga taccggaaaa tatgccgaaa tcctccgata
     1681 cacaggcggc aaccgttacg aagtttttta tcgcggtacg cactggcagg cgcaaaatac
     1741 ggggcaggaa gtgtttgaac cgggaacgcg cgccctcatc gtccgcaaag aaggtaacct
     1801 tcttatcatc gcaaaccctt aacactcgga ggaattatgg aatttttcat tatcttgttg
     1861 gcagccgtcg ccgttttcgg cttcaaatcc tttgtcgtca tcccccagca ggaagtccac
     1921 gttgtcgaaa ggctcgggcg tttccatcgc gccctgacgg ccggtttgaa tattttgatt
     1981 ccctttatcg accgcgtcgc ctaccgccat tcgctgaaag aaatcccttt agacgtaccc
     2041 agccaggtct gcatcacgcg cgataatacg caattgactg ttgacggcat catctatttc
     2101 caagtaaccg atcccaaact cgcctcatac ggttcgagca actacattat ggcaattacc
     2161 cagcttgccc aaacgacgct gcgttccgtt atcgggcgta tggagttgga caaaacgttt
     2221 gaagaacgcg acgaaatcaa cagtaccgtc gtctccgccc tcgatgaagc cgccggggct
     2281 tggggtgtga aagtcctccg ttacgaaatc aaggatttgg ttccgccgca agaaatcctt
     2341 cgcgcaatgc aggcacaaat taccgccgaa cgcgaaaaac gcgcccgtat tgccgaatcc
     2401 gaaggccgta aaatcgaaca aatcaacctt gccagtggtc agcgtgaagc cgaaatccaa
     2461 caatccgaag gcgaggctca ggctgcggtc aatgcgtcca atgccgagaa aatcgcccgc
     2521 atcaaccgcg ccaaaggcga agcggaatcc ctgcgccttg ttgccgaagc caatgccgaa
     2581 gccatccgtc aaattgccgc cgcccttcaa acccaaggcg gggcggatgc ggtcaatctg
     2641 aagattgcgg aacaatacgt agccgcgttc aacaatcttg ccaaagaaag caatacgctg
     2701 attatgcccg ccaatgttgc cgacatcggc agcctgattt ctgccggcat gaaaattatc
     2761 gacagcagca aaaccgccaa ataagccgtg atgaaaatac cgtctgaagc tctgttttcg
     2821 ggtttcagac ggtattttta tttaaacccg aacagggttg tattccgctt caaatatgtt
     2881 tcggaaccgt ctacctgccc cggtaaaaac tgccggcaaa atatttctgc gcgccctcgc
     2941 gaagcaagac caaggttacg gcggccaaag gcaatccggc caacattccg acaaagccca
     3001 tcagctctcc gaacgccatc agcgaaaaga taacccaaaa cggcgacagg ccgatacggt
     3061 ctcctacaat tttcggcgta atgaaaaaac tttcgagaaa ctgaccgacg gcaaaaaccg
     3121 cccaaacagc caagattccg ttccacgaac cgaactggag caaggctgca acagtggcaa
     3181 gcagcaatcc cgtaaacgca cccaaatagg ggacaaacac caaaataccg gcaaccatac
     3241 cgatggcaaa tcccgaatcc agtccgacta gcatcaatcc caaaccgtaa accaagccca
     3301 taatcagcat caccagaagc tgaccgcgca aaaattcgcc caatacctcg ttcaaattac
     3361 ccgtaatgcg cgtataagca ccggcaaaac gcctcggaac cagtttggcg atgccgcacg
     3421 accaccgctg ccaatccagc aggaaatagt aaagcagcaa gggcggcagc agcaggttgc
     3481 cgatactgct gacaatattg ccgccctgtt tcatcaaaac gggaaaccac gccttaagcg
     3541 cgttgctcaa ctcgcccgta tgcgcctgaa accacgcaat aatagatgcc tgatcgattt
     3601 ccacatatcc gccgattgta tttttcaacc acggcagcag cgtgttctgt ataaaaccga
     3661 ttaattgggg caggcgagat gccaaattat tgaactgccc gaccagcata gggacaataa
     3721 tcaacaataa tgccaacaac aaaatcaagg aaaacaccat cacagacatc gaagcggatg
     3781 cacggttcaa accctttttc tgcaaccatt cgaccaaagg gtccaacaca tacgccagca
     3841 ccgccgcaac cgcaaacgga gtcaaagtat cgccgagcgc gtaaaccagc cagaccaagg
     3901 cggcaaacgc cgcgccggca cccatccacg gcttgatgcc ccgtcctttt ctccgataca
     3961 taaaatccac ccttccgaaa ataatattgc gcgccagtat agcagaaccg ccgcgccgtc
     4021 aaaaaagcct ccgccttccc gatagcctgt tatcgtggtt taggctaaaa tactgcacat
     4081 ccgatgccct ctgaaaggca aacccgcctt cagacggcat accgtacacg ataaggcagc
     4141 acatcatgac cgaacaaaaa aacaaagcat ccctcatcga atttccctgc gccttcccat
     4201 tgaaagtgat gggcgcggtg catcccgagt tcgagcaggc ggtattggac accgtccgcc
     4261 ttcacgcccc cgacacgcag gcgcaccaca tcaccacgcg tccgagcagc aaaggcaact
     4321 ataccggtgc caccgttcag gtaaaggttg aaaaccaaga acaattggac aacatctacc
     4381 gcgcgctgac ttcgcacgaa ctggtcaaag tggtactttg agatgaaaat catacacaaa
     4441 ggtttggtcg aatatctgcc gacttttgaa gcgatgaaaa cctttaatgc cggacggaat
     4501 gcagacaccg aagacgaatt gtgggtcgtc gaacacccgc ccgtctttac gcaaggactg
     4561 gcgggaaaac ccgagcacct gctgattagg gacgacatcc ccgtcgtcca aatcgaccgg
     4621 ggcgggcaga ttacctatca cgggcccggg caattggtcg tttatacgat gattgatttc
     4681 aaacggcgca aaaccagcgt ccgcaacatc gtttccgcgc ttgaaaacag catcatcgcc
     4741 acattggcgg aatacggcat cgaagcggcg gcagatccca aacgccccgg catttacgtc
     4801 ggcgaacgca aaatcgcctc actggggctg cgtatcaaaa acggttccgt ctatcacgga
     4861 ctcgcactca acgtcaatat ggatttaagc ccgtttaccc aaatcaaccc ctgcggctac
     4921 gccggtatgg aaatgacgca aatcgcggat tttgtccaac cctgccccgc gccggacgaa
     4981 gtcgcctcca aactcaccgc acaccttgaa acacaactca caccgaaagc agacaacaat
     5041 gagtgaaatc aaaaccgacg accccaaacg cggcatcaaa ctcagaggcg cggacaaaac
     5101 cgcccgcatc cccatcaaag tcgtccccct tcaggaaaaa ctgaaaaagc ccgaatggat
     5161 acgcgccaaa ctcccatcgc gcaaattctt tgaaatcaaa gacattttgc gcgaacaaaa
     5221 gatgcacacc gtttgcgagg aagcctcctg ccccaacatc ggcgaatgct tcagcaaagg
     5281 cacggcgacc ttcatgatta tgggcgacat ctgcacccgc cgctgcccgt tctgcgacgt
     5341 gggacacggc cgtccgaata tgctcgaccc cgacgagccg aaaaacctcg cagaatccgt
     5401 caaagccatg aacctgcgtt acgtcgtcat cacctcggtt gaccgcgacg acctgcgcga
     5461 cggcggcgcc caacacttcg ccgactgcat caaagccatc cgcgaaacca gcccgaacac
     5521 caaaatcgaa atcctcgtcc ccgacttccg aggccgcttg gacatcgcac tgaaaatcct
     5581 tgccgaaacc ccgcccgacg tgatgaacca caacttggaa acccatccga gcctgtacag
     5641 aaaagcccgt cccggtgcca attatcaaca ttctttagac ttattaaaac gttataaaga
     5701 aatgatgccg catatcccga ccaaatccgg cattatggta ggcttgggcg aaacagacga
     5761 agacgtgcgt gaaattatgc gcgatatgcg ggcgcacaat atcgagatga ttaccatcgg
     5821 acagtacctc cagccttcag acggacactt gcctgtcttg cgctacgtta cacccgaaca
     5881 attcaaaata tttgaaaaag aagcatacga actgggcttt accaatgccg ccatcggcgc
     5941 gatggtacgt tcaagttacc atgccgacga acaggcggcg gaagccttac gcgaaagcca
     6001 cggcggttgc gggcatcatt aaactcggaa caccgttccg aagtaaaaaa taatttccta
     6061 cgctgctgtc tgccgccttg tttgatgtaa acaacatcaa tttgaattgt ccatgccgtc
     6121 tgaaagtcct tcagacggca taatttattc ccaaaatccg ataatctcaa aagaatctgt
     6181 cggttaatga gaaagattca ttataaattt tgataaaaaa ccgtgctcta tttgtttttt
     6241 gctatggtta acaagtgtaa atgttcttta ttgatgagat ggattcttag ttttagcagc
     6301 aaaaaaaaac cgataagaaa cagcatactg atttggaagg catcggggaa tacgccctat
     6361 ttattttgat aattgtttgt attttaaata gaaaatttgt catgccgaca gcttttgcat
     6421 acaatgcacg catcccgaac agcgttacag aacgccgttt aaccgcacga aacccaaatt
     6481 aagaaaagag agatattatg caaggcaatc aagctgttgt tgattacatg aacgaattgc
     6541 tgtccggcga gctggcggca cgcgaccaat acttcatcca ctcccgcctc tactccgaat
     6601 ggggctacac caaacttttt gaacgtctca atcacgagat ggaagaagaa accacacacg
     6661 ccgaagactt catccgacgc atcctgatgc tgggcggtac gccgaaaatg gcacgctccg
     6721 aactgaatat cggcacggac gtggtttcct gcctcaaagc cgacctgcaa accgaatacg
     6781 aagtgcgcga cgctttgaaa aaaggcatca aactgtgcga agaggctcaa gactatgtta
     6841 cgcgcgacct gatggttgcc caactgaaag acaccgaaga agaccacgca cactggttgg
     6901 aacagcagct gcgcctgatc gagctgattg gcgaaggcaa ctactaccaa agccaactgt
     6961 aatccgtatt aggaataaaa ggagtccgta tgaaaggcga ccgtttggtt atccgcgagc
     7021 tgaacaaaaa cttaggcttg ctgctggtaa ccattaacca atatttcctt cacgcccgta
     7081 ttttgaaaaa ctggggcttt gaagaactgg gcgaacattt cttcaaacaa tcaatcgtag
     7141 aaatgaaagc tgccgacgat ttgattgaac gcatcctctt ccttgaggga ttgcccaatt
     7201 tgcaagagct gggcaagctt ctgatcggtg aatctaccga agaaatcatc gcctgcgatt
     7261 tgaccaaaga acaggaaaaa cacgaagccc tgcttgccgc catcgccaca gcagaagcgc
     7321 aacaggatta tgtcagccgt gatttgttgg aaaaacaaaa agatactaac gaagaacaca
     7381 tcgactggct cgaaacccag caggaactga tcggcaaaat cggtttgccg aactacctgc
     7441 aaacagcggc gcaagaggac taaaacacaa accactgcca aatacagcag tcccctgcat
     7501 catcagacgg catatccgta taaacgggta tgccgttttt ctttgccggt ttacgaataa
     7561 aatctaaatt gatttgaatc aatactcgat aacggttttt gtttgataat accgatatga
     7621 aatctagatt gtttgtttgc tgtttcccaa tccgtcttga ttttacctct ttcctcttga
     7681 tgtgtgtgtg tgtttgggtg tggctgccgc cacccctttt ttttggcttt tatgtgaagt
     7741 aaaatccgta acagcaaata cttaactttt aaccatttat ccggaaacaa atgatgggca
     7801 acaaattgac tctgcctgcc gaattgcctg acgaacagga tttgcgggcg gtattggcat
     7861 acaatatgcg gcttttccgc gtgaacaagg gttggtcgca agaggaattg gcgcggcaat
     7921 gcggtttgga caggacttat gtgtcggcag tcgagcgcaa acgctggaac attgccctgt
     7981 cgaatatcga aaaaatggcg gcggctttgg gcgtggcggc gtatcagttg ctgctgccgc
     8041 cgcaggaacg gttgaacctg atgaccaatt ccgccgatac ccgacaagtg ccgtctgaaa
     8101 gcggtatttg accgccgaac catccgcctc cctttttccc gccaatcgaa agaataaaaa
     8161 tgcctgaaaa cctgtcttca gcactggaaa ccttcaaaca gcagcgcaat gccgccgaag
     8221 cgcattattt gaaagccaac cgcgtgtcgg tatttttcag agaatacacg gcggcagtcg
     8281 aaaccttgct ggcggcattg tgggcagaac attttcaaaa cagcgcgtta tgcctgatgg
     8341 cggtaggcgg cttcgggcgc ggcgaaccgt atccctgttc ggatgtggat ttggcggttg
     8401 tctcccctgt cccgctttca gacggcattc aggaacagat tgcccgattt atccaaactc
     8461 tgtgggattg caaactaatg ccgtctgtaa aaagcggcag cgttgacgaa ctgtgcgaaa
     8521 gcgtgcgcga cgatattacg ggggacacag cgtttttaga ggctaggttt ttgtttggca
     8581 accgccaaac agcagacgaa ttggcggaaa aaatgaacgt gcaacgcaat gtagcggcat
     8641 ttatcgaagc aaaattggtg gagatggaac accgccacgc caaatcgcaa ggttcggggg
     8701 cggtgttgga gccgaatatc aaaagctgtc cgggcggtct gcgcgacatc cacaccctgc
     8761 tttggatagc gaaggcgcaa ggcttggcgg ccaacctgcc cgacctgctc aaacagcgga
     8821 ttttgacacg tgccgaagcc ggtatgcttt cgcacggcta ccgccgcctc gcccacatcc
     8881 gcatccggct gcatttaaac gccaagcgcg ccgaagaccg cctgcttttc gatttgcagc
     8941 cgcaagttgc cgaaagtatg ggttatcaag atgaaaaccg ccgccgtcaa agcgaagaac
     9001 tgatgcgcgt gttttaccgt gcggtcaaaa ccgtcaaaca actgggcggc atcctcacgc
     9061 ccatgctgcg aagccgcgtt tcctccacgc cgatgcgcgt taccctgcgg attgacgacg
     9121 actacatcca agtcaacaac caaatcgccg cgcggcacac cgatattttt ttcagacggc
     9181 ccgaacacat tttcaaaatc gtcgaaatca tgcagcagcg caacgacatt actgcgctcg
     9241 aaccgcaaac cctgcgcgca tggtgggggg cgacgcgcaa aatcaaccgc agcttctacc
     9301 aaaattctga aaaccgtcgc cgcttcgccg gttttttccg cagcggcaac ggactgaccc
     9361 aaaccctgcg ctttctcaac ctctacggcg tgttgggccg ctatctgccc gcgtgggaaa
     9421 aaatcgtcgg cctgcttcaa cacgacttgt tccacatcta tcccgtggac gaccacatcc
     9481 ttgccgtcgt ccgcaacgtc cgccgccttg ccctagatat gcacagccat gagctgccct
     9541 acgcctccgc gctgatgcag tcctttgaaa aacaagacat cctctacctt gccgccttct
     9601 tccacgacat cgccaaagga cgcggcggcg accatgccgt acaaggcatc gcagacgcgc
     9661 gccaatttgc cgccgaccac ttcctgaccg aagaagaaag cgacctgctc gcctggctgg
     9721 ttgaaaacca cctccttatg tctgccgtcg cccaaaaaga agacatccaa gaccccggcg
     9781 tactcgatgc cttctgcaaa cgcgtccaaa cccacgaacg cctcagcgcg ctctacctcc
     9841 tgaccatttc cgacatacgc ggcaccaatc ccaagctgtg gaacgcctgg cgcgccagcc
     9901 tgctggaaag cctgttccac gccgccggac gctgcctcgc aggcaacgac ggcaacccgc
     9961 acgccctctt cggccgccgc cggcaggaag ccgccgactt acttacccgc gccgccgtcc
    10021 ccgaaaaaca gcagaaaaaa ctatggaacg cgctcggttc cgcctatttc gcccgccatc
    10081 agtcccgcga aatcctgtgg cacgccgcca acctcgtaca cgactttgaa ccccccatcg
    10141 tccgcagccg catcctaccc caaagcgaca gctttcaagt gatggtcttt atgcccaacg
    10201 gcccgcgcct gttcgcccgc ctctgccgca tcttcagccg ccacggcttc gatatcctcg
    10261 ccgcccgcgc ctttatcacc gaacacgact acatcctcga caccttcatc gtgcaaatcc
    10321 cctcgcagca cgcccccgaa gactaccccg acatccaaag cgcgctcgaa gccgaactca
    10381 acagctttat ccacggacac accgttgccg aaacccaaag ctgcaaccgc cgcatcagcc
    10441 gccgcagccg ctatatgcct attgcgccaa gcatcaccat cacccccgaa gaagactatc
    10501 cggaccggta ttccgtcgaa atcaccgccg tcaaccgccc cttcctgctc gccgacatgg
    10561 cggaagtctt cttcgcccac aacgtcagcc tgcgctacgc caaaatctcc acgttggacg
    10621 aacgcgtcga agacagcttt accgtcttca gccccgactt gaaaaaccca aaaatccagt
    10681 cctcattgaa acaggctttg ctggaacagt tggcataagc accctgccgg ttttcaaacg
    10741 gcatcccgcc gccacgccgt atgccgtctg aaggcggggg atattgcaaa caatataaac
    10801 tgtcaagccg ccggcaacgt gctataatgc cgtgcatctt gcacaaaccg aaagaccgag
    10861 cattatgcgt atcgtagaaa aagcctatac tttcgacgat gttttgttgg ttcccgcaca
    10921 ttcgaccgtg ctgccgcgag acgttaaact tcaaaccaag cttacccgcg aaatcacact
    10981 caacctcccc ctgctttccg ccgcgatgga taccgttacc gaggcgcgcc tcgccatttc
    11041 gatggcacag gaaggcggca tcggcatcat ccataaaaac atgccgcccg aaatgcaggc
    11101 gcgcgccgtt tccaaagtaa aacgccacga aagcggcgtg gtcaaagacc ccgtaaccgt
    11161 tgcgccgaca acgctcatcc gcgaagtctt ggaaatgcgc gcgcagcgca aacgcaaaat
    11221 gtccggcctg cccgtcgttg aaaacggcaa agtcgtcggc atcgtaacca accgcgacct
    11281 gcgttttgaa aaccgcgtcg atttgcccgt ttccgccatt atgaccccgc gcgaacgtct
    11341 ggttaccgtc cccgaaggca caagcataga cgaagcgcgc gaactgatgc acacgcacaa
    11401 agtcgaacgc gttttggtcc tgaacgaaaa agacgagctt aaaggcttga ttaccgttaa
    11461 agatatttta aaaaccaccg agtttcccaa tgccaacaaa gactccgaag gccgtctgcg
    11521 cgtcggtgcg gcagtcggca ccggcggcga caccgacgag cgcgtcaaag ccttggtcga
    11581 agccggcgcg gacgtgattg tcgtcgatac tgcccacggt catagccaag gcgtgatcga
    11641 ccgcgtgcgt tgggtcaaag aaacctatcc gcacatccaa gtcatcggcg gcaacatcgc
    11701 cactgccaaa gccgcattgg atttggtcac cgtcggcgcg gatgccgtca aagtcggtat
    11761 cggtccggga tcgatttgca ccacccgtat cgtggcaggc gtgggcgtgc cgcaactgac
    11821 cgccattcac aacgttgccg aagccctcaa aggcacgggc gttccgctga ttgccgacgg
    11881 cggcatccgc ttctccggcg acatcgccaa agccctcgcc gcaggcgcgt acagcgtcat
    11941 gctcggcggt atgtttgccg gcacggaaga agccccgggc gaaatcgaac tctaccaagg
    12001 ccgctcgtac aaatcctatc gcggtatggg ttccttgggc gcgatgagcc aaggttctgc
    12061 cgaccgctac ttccaagaca aaaccgacag caccgacaaa tacgtccccg aaggcatcga
    12121 aggccgcgtt ccctacaaag gtccgattgt gaacatcatc caccaactga ccggcggact
    12181 gcgttccagt atgggttatt tgggttgcgc caatattgcc gaaatgcacg aaaaagccga
    12241 atttgtggaa atcacttccg caggtatgag cgaatcgcac gttcacgacg ttcaaattac
    12301 caaagaagcg ccgaattacc accgctgatt tgaacagcct ttcaggacaa atgccgtctg
    12361 aaacctaatt tccgagtttc agacggcatt tttgttcgtt caaggcaagc cgcacgggct
    12421 gattgcgcgg acgggcaaag ccattctgac ggacacgccg aaagaatgaa aatgccgtct
    12481 gaacgccgtt tgcaccccgt ctgccgaata ccgtcggcct ttaaaacggc ggtggaaata
    12541 aacgcaatct ttcaatacgg gcgtttcgat tggtcggaaa tgatgcgccg gccgccatat
    12601 caaaaaatcg ggcggcggct tcgggaaagt atgccccgcc ctccccaagc cgccccgtca
    12661 gcattatgat ttgcctttcc gttttttgac cttaatcgga accggtttct ttgccttttt
    12721 ccccgattcg gcaacggcag gcacgcccct tccccttact ttgccgcatc gggctgtttt
    12781 tttctcggcg gtggttttcg atttcccttt ccccgccgcc cctgccggtt tggcagatgc
    12841 ggataatttg accttccgcc gcctgccgct ttctccggca attaggacaa agtcgatttt
    12901 tccatcatcc aaatcggcac gcgcgacccg gacggcaacc ctgtccccca tattgaaacg
    12961 gatgccgctg cgttcgcctt cgattgccat gatttcgggg cggaagttga aataatcttc
    13021 gcccaaatcg ctgatatgta ccagaccgtc gatatggata tcgtccaaag tgacaaatat
    13081 tccaaaattt gccaccccgg agattttgcc ttcaaatatt tcaccgacct tatcgcgcat
    13141 ataataagtt ttcagccagt tttccacatc gcggccagca tcgtcggcac ggcgttcgca
    13201 aaacgaagta tgcacgccca aagcctgcca gcttttgttt ggcgtgtagg ttttccggtt
    13261 caatacggct ttgatggcac ggtggacggt caggtcggga tagcggcgga tgggcgaggt
    13321 aaagtgggcg tatgcttcat aagccaaacc gaaatgccct tcgcaatgcg gttcgtaaac
    13381 cgcctgctgc atggagcgca acatcatgac ttgcagcaat tcggcatccg gcctgccttt
    13441 gaattgttcg gcaagcgcgg catagtcttt cggcgacggg ttgtcgccgc cgccaagttg
    13501 aagccccaac agaccgagct gctcgcgcag ggtggcgagt ttttcgggcg tggggcccaa
    13561 atggttgcgg aacaaagccg tatgtttgtt tttcaacaga aaatccgccg cgcaaacatt
    13621 cgccgccagc atacattctt caatcagctt gtgggcatcg ttgcggacga cggggacaat
    13681 tttttcgatt ttgccgttgt cgtcgaaaat catctgggtt tcgacgcttt caaactccac
    13741 cgccccgcgc gccagacgtt ttttctgcaa aattttaaac agcttgtaaa gcgtgtcgat
    13801 ttgggctttg tgcggattcc cgatgccgtc tgaaagccat ttccaaactt ggttgtaggt
    13861 caggcgggca tgagagcgca tcacggcggg atagaagcgg tattctttga tattgcccgc
    13921 ataggtaacg accatatcgc acaccataca caaacgctcg acatcgggat tgagcgagca
    13981 gatgccgttg gacaggtttt ccggcagcat cggaatcata cggcgcggga aatacacgct
    14041 ggtactgcgt tcttgagcat ctgcatcaat cgcatcgtca gggcggacat aatggctgac
    14101 atccgcaatc gccacgacca gacggtaatt gcgtccgact ttttcggcaa acaccgcgtc
    14161 gtcgaaatcg cgcgccgttt cgccgtctat cgttaccaaa ggaaggtcgc acaaatcgac
    14221 gcggcctttc aaatcgcttt tgcgtacatg gtcgggaatt tttttcgcgg atttggcaca
    14281 cgcttcactg aatcggtgcg gcaaatgatg cttgcgcacg gcaatttcga tttccatccc
    14341 gctgtcggca taatcgccca aaacttcaat gatttttgcc actgcaggcc ggttttgctc
    14401 gggataaacc tcaattttgc cgacgataac ctgaccgaat tcgggtttga aacgcgccac
    14461 gccgtccggt tccaacacga tgctttggtt cagacgcttg tcttccggct ccaaaatcgc
    14521 cacgccccta tccatataga aacggccgac aactttgctt tgcgcgcgtt cgacaatatc
    14581 cagaaccgtc ccttcgcggc ggcccctgcg gtcgataccc gcaggacgga cggtaacggt
    14641 gtcgccgtgc atgacaccac gcatctggcg ttcgtataaa acgaaatccc cttcgtccat
    14701 cggcatgagc ggcacggcga agccgaaacc gtccttatgc gcctcgacgc ggcatttgac
    14761 caaatccagc ttgtccgccg cgcaaactgc gcctcggcgg ttgattaaaa cctgaccgtc
    14821 ccgcgccatc gccttcagac ggcgttcaaa aaagacatac tcgtcttccg taatcgacag
    14881 ctcgcgtgca agcgattcga tttttgaagg cacacctttg cgctccaaca attcgattat
    14941 ccattcccga ctgggcaaag gatgttcata acgctgtttt tcacgactta aaaacgggtc
    15001 tttttcccgt aaatttaaag atttaatatt tttattcatt ttgactgttg acattctctt
    15061 tatgaactat ataatagcga cttctttgcg gcaggacact gtaccgcaaa acagcaaata
    15121 aagcaaagcc cgggtggcgg aattggtaga cgcgccagct tcaggtgctg gtatcctcac
    15181 gggtgtggaa gttcgagtct tctcccgggc accaaataac aaattatccc gattaggata
    15241 atttccgcca ttagagaggt ggatgagtgg tttaagtcgc acgcctggaa agcgtgtata
    15301 cgtgaatagc gtatcgaggg ttcgaatccc ttcctctctg ccaagtacaa atcccaaact
    15361 tttacagttt gggatttttt gttacttacg tattgatttt attgcgtaaa atcagctaca
    15421 acctattacc ctacgttacc attgattact atccgttacc gtttttttat ggtatgtttt
    15481 gcggtatgtt ttatcctctt cctaagcctg taaggactca aaaggcgtat cccttcaaac
    15541 gatattgttc gacatgctgc cgggaaaccg aacccgatac cgaaacgcga cggggcgata
    15601 tgccgctgga aaaatggcgg cagtttcttg attggatagg aaaaacgccg tctgaaacag
    15661 cttcaagaag ccgcaaagcc cgtccgaata aaaaataccg tctgaaattt ttcagacggt
    15721 atcgcgtatc atttaaacat taatccagct ttttaaaatg acggcggcgt tccaattcgc
    15781 tcaggtaacg cttgcgcagg cggatggatt gcggcgtgat ttccaccagc tcgtcatcgt
    15841 cgataaactc gaccgcgcct tccagcgtca gtttgatcgg cgtggtcagg cgcaccgctt
    15901 cgtcggtacc gctggcacgg atattggtga gttttttgcc tttgagcggg ttgaccacca
    15961 aatcgttgtc gcggctgtgg atgccgataa tcataccttc gtagattttg tcgttgggcg
    16021 atacgaacat acggccgcgg tcttcaagat tccacaaggc gtaagcaacc gcctcgccct
    16081 gctcttggga caccagtacg ccgttgtggc ggccgggcat atcgggtttg acgggcgcgt
    16141 agtcgtcgaa cacgtggctc atcagcccga cgccgcgcgt cagggtcatg aattcgcctt
    16201 ggaaaccgat caagccgcgc gctggaatat ggtattcgag gcgggtgcgt ccgttgccgt
    16261 cgctttccat attggtcagt tcgccacggc ggcggccgag ttcttccatt accgcgcctt
    16321 ggttgtcgtc gggtacgtcc acagtcaggt tttcataagg ttcgcatttt tgaccgtcga
    16381 tgtctcggta cacgacgcgc ggcttgccga cggcgagttc gtagccttcg cggcgcatat
    16441 tttccagcaa aatcgtcagg tgcagttcgc cgcgcccgga tacgcggaac acgtcggcat
    16501 cggcggtgtc ttccacgcgc agggcaacgt tggtcagcaa ttctttttgc aggcggtcgc
    16561 ggatttggcg gctggtcacg aatttgcctt ctgtacctgc gagcgggctg gtgtttacca
    16621 taaagtccat cgtcagcgtc ggttcgtcca cgctcaacat cggcaggcct ttggggttgt
    16681 ctttatcggt gatggttacg ccgatgccga tgtcttcgat accggaaata atcacgatgt
    16741 cgccggcttc ggcttcttca agcggcacgc gttccaagcc tttgaaaccc aaaagctggt
    16801 tgatgcggcc ttgggcgatt tgctgctcgt ggttcatcac ggcaacggtt tgaccgggtt
    16861 tgatgcgtcc gttcaagata cgaccgatac cgaggcggcc ggtgtagttg tcgtagtcga
    16921 gttgggaaat ttgcagttgc agcggctcgt ccgcgctgcc gctcggtgca ggcgtgtatt
    16981 ttaggatggt gtcgaacagc gggcgcatat cgctgctctc gtcggtttct tccagcttgg
    17041 caaagccgct caaacctgaa gcgtaaacaa tcgggaaatc caactgctcg tcggtcgcac
    17101 ccaagttgtc gaacaactcg aaagtctggt cgataaccca gctcggacgg gcggacggtt
    17161 tgtcgatttt gttgatgacg acaatcggtt tcagccccaa agccaaggct tttttggtca
    17221 cgaaacgggt ttgcggcatc ggaccttcct gtgcgtccac caacaagacg acgcaatcca
    17281 ccatccccaa aacgcgctcc acttcgccgc cgaagtcggc gtgtcccggc gtgtcgacga
    17341 tattgatgtg gcagccttcg taatcgatgg cggtgttttt ggcgaggatg gtgatgccgc
    17401 gttctttttc aaggtcgttg ctgtccatca cgcgctcgtc aacctgctgg ttggcgcgga
    17461 atgtgccgga ttggcgcagc agttggtcga ccaatgtggt tttgccgtgg tcgacgtgtg
    17521 cgatgatggc gatgttgcgg atttgtttca taattaatgt ttttcaaaaa ctgtaagaga
    17581 taactgcgta ttataccacg atttaccgga cagtatttgg aaaatgaaga tgccgtctga
    17641 agcccttgcc tttcagacgg catcttctta catactttca gttatccagt tgcgcagcca
    17701 ccttgccgcc ggggcgattt cccctgccga cagcccagcc gcaatgcctg ccgattcctt
    17761 tataacatcc gccgccgcgc cgtgcagcca cgcgcccgcg caggcggctt caaaaaccgg
    17821 cacgccctgt gcgagcagac tgccgatgat gccgcccaat acgtcgccac tgcccgccgt
    17881 tgccaatccc gcattgccgc tttcgttgac atagatttcc gtatcggacg cggcaaccaa
    17941 tgttttgtgc ccctttaaaa ccacggttgc accgagaatt gcccctatct tccttactgc
    18001 cgccgtccga tccgcctgaa cctgtgcaac tgtcgttcca agcaggcgcg cggcttcggc
    18061 ggggtgcggc gttaaaatca ggtttttaca cccgcgcgcc agatttcggg tttcggcatc
    18121 ggttgataat atgttcagcg cgtccgcatc caaaacgacg gcttcgtctc gattccgcgc
    18181 cagcactgcc gccaccgttt ccactgccgc cgcatctgta cccaagccgc aacccgccgt
    18241 ccaagcgttt atgccttgac gcttggtcaa accgtctgcc gtgtccaaca taatctcggg
    18301 aaaaccggca atgacggcaa aaggcagcgt atcctgattg aaacctgccc gcaccttacc
    18361 gcagccgaga tacattgccg ccgatgccgc caatacgggc gcgccgctca tccctgccga
    18421 tccgccgact acggcgagcg tgccgaaagt ccctttgtgc gaatcttccg cacgggcttt
    18481 gaaaacatgg ggaaaacgca atgccgtctg aagcatccgg cggcggcttt cgcctgaaag
    18541 atggaaaacg ggaaacattt cccctccaat cggttaaaat taaggctcga aaacaaaccg
    18601 attctaccgc cggaaaccga ttatgaaaaa caaaaccaaa gtctgggact tccccacccg
    18661 ccttttccac tggctgcttg ccgcatccct gccctttatg tggtatagcg caaaagccgg
    18721 cggcgatatg ctgcaatggc acacgcgcgt cgggctgctc gtccttttcc tgctcgtatt
    18781 ccgcctctgc tggggcattt ggggcagcga taccgcccgt ttctcccgtt tcgtccgagg
    18841 ttgggcaggt atacgcggct atctgaaaaa cggcattccc gaacatatcc agcccggaca
    18901 caaccccttg ggcgcactga tggtcgttgc gcttttggcc gccgtctcat ttcaagtcgg
    18961 cacggggctt tttgccgccg atgaaaacac cttcagcacc aacggctacc tcaaccattt
    19021 ggtttccgaa catacgggca gccttatacg gaaaatccac ctcaactttt tcaagctgct
    19081 cgccgttttt tccgcagtcc acatcgccgc cgtcgccgca taccgcatat tcaaaaagaa
    19141 aaacctcgtc cgcccgatga taaccggctt caaatacatc gaaggcaaaa cctcaatccg
    19201 ctttgccggc aaagccgcgc ttgccgccgc attatcggtt gccgcgcttg ccgcagccgc
    19261 catcctgctc ctgtcctgaa acccgacatc atgccgtctg aagccctttt ctgcctttct
    19321 gacggcattt tccccaccgc acaaacggtc tgaatgcccg aaacggcttc aggccgtttt
    19381 tccgtccaaa accgttatat agcggattaa caaaaatcag gacaaggcgg cgggccgcag
    19441 gcagtacgaa tggtacggaa ccggttcgcc cggtgcttgg gcgccttagg gaaccgttcc
    19501 ctttgagccg gggcggggca acgccgtacc ggtttttgtt aatccgctat aaaacggaaa
    19561 accgccgcat ccgcagccgc cccccatata gctttcagct tctaacaaat gcctttttat
    19621 tatttaaaat gacccgcatt ccgacaaaaa tgccgcgacg caccgtatcg gaaacaaggt
    19681 gcgggcaaac agcaaaggag tcagccagtg aattactttc ccattttcgc caacctctcc
    19741 ggccgccccg tattggtcgt aggcggcggc gcggtcgccg cacgcaaaat cagcctgctg
    19801 ccgaaggcgg gcgcagaggt cggggttgcc gcaaaacacc tgaatgccga actctccgcg
    19861 ttggcggcgg aaaacaaaat cctgtggctt gccaaagaat ttcgtgccga acacatccgc
    19921 acggttttcc tcatcattgc ggcaagcagc gaccaagccc tcaaccggcg cgttttccat
    19981 cttgccgaaa gctgccaaaa gccggtcaac gtggtgggcg accgtgacca ttgcagcttc
    20041 atcttcccgt cggttatcga ccgcgaccct gttcagattg ccgtttccag ctccggcagc
    20101 gcgcccgtcc tcgcccgcct gctgcgtgaa aggctggaag ccttgctgcc gccgtctttg
    20161 ggcgatatgg cggaaatttc aggaaggtgg cgcgatgccg gtttttattt ccccgtccaa
    20221 ctggtcgtcc gccaagatgc cgacaaggcc gacggtttcc gaggctatca ggggcgcatc
    20281 gaacgcggtt cggtcaccgt cgggcaaacc gtccgcatcg aaccgaacgg gctgaccgcc
    20341 gaagtgtccg aaatcatcgc gccgaaaggg gaagtggcgc aggcttttgc cggcgaggcg
    20401 gcaaccatcc ggctggaccg cgacatcgat gtttcgcgcg gcgatctttt tgtcgataaa
    20461 aatttcccgc tcgccccgca aaaacatccg gaagccacgc tttgctggtt tgacgaacgc
    20521 ccgctcaaca ccgcgcgcaa atacctgctc aagcacggca cgcaaaccgt gccggcaaaa
    20581 gtcggggaaa ttgaaagcgt tttggatgtc cgcacgctgg aacaagaggc cggcgcggaa
    20641 tccttaaaga tgaacgacat cgccaaagtc cgcatcaacc ttcaaaaacc cgttacggca
    20701 acgccttatg cggaaaacac cgccgccggt tcgtttatcc tgatagacga agcgacatac
    20761 ggtactgttg cggcaggtat gattttatga gtgaacacga tatgcagaac acaaatccgc
    20821 cattaccgcc tatgccgccc gaaatcacgc agctcctgtg tcggggctgt ccggctacgc
    20881 ttgggcaaaa gcaggaaacg gggcatccgc agggctgccc gcgcttcaga cggcattgcc
    20941 ggcggcagaa cctttttccg taaccgtcct ttccgcctcg caaaccggca atgcgaaatc
    21001 cgttgccgac aaagcggcgg acagcctgaa agccgccggc atccaagtca ggcgcgccga
    21061 actgaaaggc tacaaggcga aaaacatcgc cggcgaacgc cgcctgctgc tggttacgtc
    21121 cacccagggc gaaggcgaac cgccggaaga agccgtcgtg ctgcacaaac tgctgaactg
    21181 caaaaaagcc ccgaaattgg acaaactcca atttgccgta ctgggtttgg gcgacagctc
    21241 ctatccgaat ttctgccggg cgggcaaaga tttcgacaaa cgttttgaag aattgggcgc
    21301 aaaacgcctg ctcgaacgcg ttgatgcgga tttggacttt gccgccgccg cagacggatg
    21361 gacaggccgc attgtcgcgc gcttaaaaga agaagccgca aaaaaccggg caacgcccgc
    21421 gccgcaggca acgtcccccc gccggccttc agacggcatc ggagggcagg tactgcaagg
    21481 cagacccctt tcccgccgcc ctgctggcca atcagaaaat caccgcccgc caatccgata
    21541 aagacgtgcg ccacatcgaa atcgatttga gcggttcgga tttgcactac ctcccgggcg
    21601 acgcgctcgg cgtttggttt gacaacgatc cggcactggt cggggaaatc ctagacctgc
    21661 tcggcatcaa tccggcaacg gaaatacagg cgggcggaaa aaccctgccg gttgcctccg
    21721 cactgttatc ccatttcgaa ctcacgcaaa acacccccgc ctttgtcaaa ggctatgcca
    21781 cgttcgccga tgatgacgaa ctcgaccgta ttgctgccga caacgccgtt ttgcaaggct
    21841 ttgtgcaaag cacgccgatt gccggtgtgc tgcaccgctt cccggcaaaa ctgacggcgg
    21901 aacaattcgc cggcctgctg cgcccgcttg cgccgcgcct gtattcgatt tcctcgtcgc
    21961 aggcggaagc gggggacgaa gtgcacctga ccgtcggcgc agtgcgtttc gaacacgaaa
    22021 ggcgcgccag gtcgggcggc gcatcgggtt tctttgccga ccggctggaa gaggacggca
    22081 cggtgcgcgt gtttgtggaa cgcaacgacg gcttcaggct gcccgaagac agccgcaagc
    22141 cgattgtgat gatcggctcc ggtaccggcg tcgcaccgtt ccgcgccttc gtccaacaac
    22201 gtgccgcaga aaatgcggaa ggcagaaact ggctgatttt cggcaatccg cattttgccg
    22261 ccgacttcct ctatcagacc gaatggcagc agtttgccaa agacggcttc ctgcacagat
    22321 atgacttcgc ctggtcgcgc gatcaggaag aaaaaatcta tgtgcaggac aaaatccgcg
    22381 aacaggcgga aggactttgg caatggctgc aggaaggcgc gcatatctat gtgtgcggcg
    22441 atgcggcaaa aatggcaaaa gaagtggaag ccgccttgct ggatgtgatt atcggggcag
    22501 ggcattcgga cgaagacggc gcagaaggat atttggatat gctgcgcgaa gaaaaacgct
    22561 atcagcgtga tgtttattga ttaaatataa tcgggaggaa cacaaaatga ccgtacaggc
    22621 caagacaaaa ggtttggcgt ggcaagaaaa gccgctatcc gacaacgaac gtctgaaaac
    22681 cgaaagcaat tttttacgcg gcacgatttt ggacgatttg aaagacccgc tcacgggcgg
    22741 cttcaaaggc gacaacttcc aactcatccg cttccacagt atgtatgagc aggatggccg
    22801 cgacatccgc accgaggcaa aactcgagcc cttgaaattt atgcttttgc gctgccggct
    22861 gccggcggga tcatcaaacc gtcccagtgg atagaactgg acaaattcgc ccgggaaaag
    22921 agtcattacc gctccatccg gctaaccaac cggcaaacct tccaatttca cggcgtgccg
    22981 aaaaccaagt tgcagacgat gccccgcctc ttgcacagcc tgggtttgga ttccatcgct
    23041 acggcggcgg atatgaaccg caatgtgctt tgcacctcca acccgatcga gtccgagctg
    23101 caccggcagg cttacgaata cgcgaaaaag atttccgaac acctgctgcc gcgcacgcgc
    23161 ggttatctgg atatgtgggt ggacggcaaa aaagttcaaa gttccgacga cttccttcaa
    23221 gaaggcgagc cgattttggg caaaacctat ctgccgcgaa aattcaaaac cgcagtcgtc
    23281 atcccgccct tgaacgatgt ggactgctac ggtaacgatt tggatttcgt cgccgtttca
    23341 gacggcaacg gacagcttgc cggcttcaat gttttggcgg gcggcgggct ttcgacggaa
    23401 cacggcaacg gcaacaccaa aacctatccg aacatttcac tggaactggg tttcgtgcct
    23461 ccggaacacg cactgaaggc cgccgaagcg gtggtaacca cgcagcgcaa cttcggcaac
    23521 cgcagccacc gcaaaaacga ccgcacccgc tacaccattc aaaatatggg cttggacaac
    23581 ttccgcacgg aagtcgaacg ccgtatgggt atgccgttca aacccatacg gccgttcaag
    23641 tttaccgggc gcggcgaccg catcggccgg gtgaaaggca tagacggcaa ctggcatttg
    23701 acccttttca tcgaaagcgg gcgtttggtt gacgaaggcg ggaaacagct tctgaccggc
    23761 gtgttggaaa tcgccaaaat ccacaaaggc ggcttccgca tcaccgccaa ccaaaacctc
    23821 atcgtggcaa atgtgcccga aggaggcaaa gcccggatcg aacagctcgc ccgctcatac
    23881 gggctgatac gcgacggtgt cagcaagctg cgcgaaaatg cgatgtcgtg cgtttccttc
    23941 ccgacctgcc cgctggcaat ggcggaagcc gaacgcgtgt tgccggactt catcggcgag
    24001 ctggataaga ttatggcgga acacggcacg tcggacgact acatcgttac ccgcattacc
    24061 ggctgcccga acggctgcgg acgggcgatg ttggcggaaa tcggactggt cggcaaagcc
    24121 gtgggacgct acaacctcca catcggcggc gaccgcgaag gcgtacgcat cccccgcctc
    24181 tacaaagaaa acatcaccct gccggaaatc ctttccgaat tggaagacct gatcggcaaa
    24241 tgggcggcgg gacgcgatac cgacgaaggt ttcggcgatt tcgccatacg gacgggcatc
    24301 gtcaaacccg tgttggatgc gcccgtcgat ttttgggacg catccaaagc cgtcccgatt
    24361 gcgcgcgcct gaacccgtcc cccgcattcc tgcggggatg ttcttttttg ccgcacacgt
    24421 ttccattccg caaaattccg atacttggta taaaataccc ccttttccca ctctaaaaaa
    24481 ccgtacccga tgccgtctga aacagccctg cctttcagac ggtataggca caaggaaaca
    24541 cactatgcca gaataccgct ccaaaacctc cacccacggc cgcaatatgg cgggcgcgcg
    24601 cgcattgtgg cgcgccaccg gcgtgatgga aaccgacttc ggcaagccca tcatcgccgt
    24661 tgccaactcc ttcacccaat tcgtgcccgg ccatgtccac ctgcacaata tgggccagct
    24721 ggttgcccgc gagattgaaa aagcgggtgc aattgccaaa gaattcaaca ccatcgccat
    24781 cgacgacggc atcgccatgg ggcacagcgg tatgttgtac tccctgccca gccgcgattt
    24841 gattgccgac tccatcgaat atatggtcaa cgcccactgc gccgacgcgc tggtgtgcat
    24901 ttccaactgc gacaaaatca ccccgggaat gctgattgcc gccatgcgcc tgaacatccc
    24961 caccatcttc gtctccggcg gcccgatgga agcgggcaag gtcatcggcg tggcaaacat
    25021 ccaacccgaa cgccgtttgg acttgattga cgccatgatc gaatcggcgg acgacaatgt
    25081 cagcaaccgg caagtcgaag aagtcgaaca aaacgcctgc ccgacctgcg gctcgtgttc
    25141 gggtatgttt acggcaaact cgatgaactg cctgaccgaa gcgctcggtc tgtccctgcc
    25201 cggcaacggt tcgtatttgg cgacccacgc aggccgcaaa gaattgttcc tcgaagcagg
    25261 gcgtatgatt gtcgaaatca ccaaacgcta ttacgagcaa gacgacgaaa ccgtgctgcc
    25321 gcgcagcatt gccaccaaaa aagcgtttga aaacgccatg acgatggata ttgcgatggg
    25381 aggcagcacc aataccatcc tgcacttgct cgccgtcgcc aacgaagcgg gcgtggattt
    25441 caaaatggca gacatcgacc gcttaagccg cgtcgtgccc tgcatctgca aaaccgcgcc
    25501 caacaaccac gactactata tggaagacgt gcaccgcgcc ggcggcatct tcgccattct
    25561 gaaagaactt gacaaagcgg gcaaactgca caccgacgtg tacacgatcc acgcgccgac
    25621 actgaaagac gcgattgaaa aatgggacgt caccaatccc gaaaacaccc gcgccatcga
    25681 acgtttcaaa gcggctcccg gcggcgtgcg caccactcag gcgttctcgc aaaaccgtat
    25741 ttggaaaacc ctcgaccttg accgcgaaaa aggctgtatc cgcgacgtgg cacacgccta
    25801 ctcgcaagac ggcggtttgg cggtcttatt cggcaacatc gccgagcgcg gctgcgtggt
    25861 aaaaaccgca ggcgtggacg agagcatcct caaattcacc ggccgcgcgc gcgtgtttga
    25921 aagccaggaa gccgccgtcg aaggtatttt gggcaaccaa atcgtcgccg gcaacatcgt
    25981 catcatccgc tacgaaggcc cgaaaggcgg cccgggtatg caggaaatgc tatacccgac
    26041 ctcctacctg aaatccaaag gcttgggcaa agcctgcgcc ctcttaaccg acggacgctt
    26101 ctccggcggc acatcaggtt tgtccatcgg acacgcctcg cccgaagcgg cggaaggcgg
    26161 cgcaatcggt ttggtacacg aaggcgatac gattgaaatc gacatcccca aacgcagcat
    26221 ccgccttgtc atttccgatg aagagcttgc cgcacgccgt gccgaaatgg aagctcgcgg
    26281 cagcaaagcg tggaaaccgg aaaaccgcga ccgctacgtc tccgccgcat taagggctta
    26341 cggcgcgatg gcgacttccg ccgacaaagg cgcggtgcgc gacgtatcgc aaatcgagag
    26401 ataagcccca aaacgtgcgg caaatgccgt ctgaacagta aaggcatgat tattaacaaa
    26461 tttatctggg cgatggcggt attttccgca attttggccc tcgttatcgg cagcggcttt
    26521 accgcgctcg agtatgtaaa agagccgcct gccgcccctt atgctgtttc cgcgccgaaa
    26581 accgccgggg tcaagcctcg gcgcatcctg ccggaacgga atcccccgtg ctgccgattg
    26641 acggttcaaa ccccgcgccc gtttcaaaca ccggcgatgc ggacggacag gatacgcctg
    26701 acggaaaggc agcagatacc gtttccatca tcggggtcgg cggcattatg ctcggcagca
    26761 attatccggt cgattacccg cccgatacca atatcctgaa acacgtcgaa tccgtcttgg
    26821 aagatgcgga cattaccgtc ggcaaccttg aaggtacgct gtttgacgaa ggcggtacgc
    26881 cgaaaaaatg tgcaaaccac caaaatatgc tatgcattcc gaacgccctc cgcatacggg
    26941 caataccttg ccgacgcggg attcgactac ctcagcctcg caaacaacca cagcaacggc
    27001 ttcggcgcgc aaggcatcac ggcaacggca ggcaaccttg acgaattgaa catcaaatat
    27061 tcgggcatcg aaaacaggtt tgaaaccgcc atcctgaaga aaaacggcgt gagatacggc
    27121 ttcgtctcct tcgcccccaa ccttgccgcc gtcaaactga acgattacgc caaattcaaa
    27181 aaactgattc ggaaaaccaa acaaaaaacc gacatcgtca ttgtgatgtt ccacggcggc
    27241 gcggaaggga aacaggctga acacctgccg ttcgataccg aaatcttcta tggggaaaac
    27301 aggggcaacg tcgtcgagtt tgcgcggctc gccgtcgatt ccggcgcgga tgtcgtattc
    27361 gggcaggggg cgcacgttac tcgcgccgtc gaactttacc acgaccgctt catctcttac
    27421 agcggcggca actttgccac ctacggcatc agcggcattg cgccgatttt caaaattatc
    27481 accgacaaac aggggcggtt cgtttccggc aacattatcc ccatcactca agtcggcgat
    27541 aaaatcccca aaatcgaccc tgaaaaaact gttattgagc ggattattta tctgaaccat
    27601 agcgacttcc ccaacgggaa cggactggat gtctcgcccg gcggcgacat cacgcgccgg
    27661 taaaaagata ttggaaattc cgtccgaaca aatgccgtct gaaagccttt tttcaggttt
    27721 cagacggcat tcaagtttca gacggcattt ttcaatcgtc aataatccgc tttaatcccg
    27781 gaggcttttt ccacttcggc gacctcgcct tccggctcaa actgcaaggg atacaggtta
    27841 agcttttcca tcaggattct atcgccgtcc tcatcaggat tgcctgtggt caacagcttg
    27901 tcgccgtaaa aaatcgagtt cgcacccgcc atgaagcaca tcgcctgcat cgcttcaggc
    27961 atattgctgc gccctgccga gaggcggaca taactttgcg gcatcgtaat ccgcgctacg
    28021 gaaaccgtac ggacaaattc ggtccaatcc aaatcttcgg catcggcaag cggcgtgcct
    28081 tccactttga ccaaccggtt aatcggcacg ctttcgggct gcgggtcgag gttggcgagg
    28141 ctggcaatca gcccggcgcg ttcggcgcgg gtttcgttca tcccgacgat gccgccgcag
    28201 cagactttca aaccggcgtt gcggactttg cccaaggtat ccatacggtc ttcgtgccgg
    28261 cgtgtgtgga tgatgtcgtt gtagcggtcg gggtcggtgt cgaggttgtg gttgtaataa
    28321 tccaagcccg cctctttcaa gtcttccgcc ataccttctt cgagcatacc gaacgtgccg
    28381 caggtttcca tacctaagcc cttgacggct ttgatgatta cggaaaccgt ctccacgtct
    28441 ttgggtttgg ggccgcgcca tgccgcgccc atacaaaacc ggcttgcgcc gcgcgatttg
    28501 gcgattttgg ctttttcgac gatttcatcc acatccatca tctgctcttt gcccaaattg
    28561 gtgttgtggt gcgctgattg cggacagtag gcgcaatctt cgggacaacc gccggttttg
    28621 atggacaaca gcgtggaaag ctggatttcg cgcgggttga aattttggcg gtggacttct
    28681 gccgcctgat agacaagttc taaaaacggc agtccgaaaa gtgcctcgac atcgcatttt
    28741 ttccaatagc gcgcggtggg atgaggcttg cactcggtct tacggcgcaa ggcgacgggg
    28801 gatacggtca taatgtgttc tttcgtattt acagcggcgc aatgacgctg acaggcaaac
    28861 cgccggcgca atgacggcgg ttgcgggtaa tcaaatggct gaaaacagcg gcggcaagtg
    28921 taacgcgttt gaaaatacgg ggcaaacggt ttttccgcct ttgtgccgcg cagccgggca
    28981 atgccgtcta aagggctttc agacggcatt gcccgggcta tctcaaagcg tccagcagtt
    29041 tggtgtgtat tccgccgaaa ccgccgttgc tcatcaccaa aatatggtcg ccggttcggg
    29101 cgtttttcac aatttcggca acgaaggtat cgaaatcttt accgacgcgc agcctgcagc
    29161 ccaaaggcgc gagggcttcg gcaacgtccc agtccgcgcc gccggcgtag cagaacactt
    29221 ggtcggcttc tttgaggctt gcgggcaggg cggacttcat cgtgccgagt ttcatggtgt
    29281 tggaacgcgg ctcgaggacg gcgaggatgc gcgcgccgcc gacacgttgg cgcaaacctt
    29341 gaatcgtggt ttcgatggcg gtcgggtggt gggcgaaatc gtcgtaaacg gtgatgccgt
    29401 ttgccgtgcc tttgatttcc atgcggcgtt tgacgttttt aaacgcaccc aaggcttcgc
    29461 aggccgtctg aacatcgact ccggcatggc gtgcggcagc gatgacggcg agcgcgttca
    29521 tgcggttgtg tccgcccatc aaatcccatg cgacgtgtcc ggcttttttg ccgtcaagca
    29581 atacgtcgaa cgagccgtcg gcattgactt caccaatctg ccagccgtgt ccggtgccga
    29641 atttttccac cggcgtccag cagcctttgt ccaaagtatc ttgcaggctt tgctgctgtc
    29701 cgttgcagac gatgaggcct tcggatggta cggtgcgcac gaggtggtgg aactgggtct
    29761 gtatcgcgcc caagtcggcg aagatgtcgg cgtggtcgaa ttccagattg ttcaacacgg
    29821 cggtacgcgg gcgataatgc acgaatttgg agcgtttgtc gaaaaaggcg gtgtcgtatt
    29881 cgtcggcttc gatgacgaaa aacggcgatt tgctgttcgg gtcttgacgc ggcgtttgcg
    29941 gtaggcgggc ggaaacgccg aaattttccg gtacaccgcc gatgaggaag cccggcgcga
    30001 gtccggcata ttccaagacc caggcgagca tggacgcggt ggtcgttttg ccgtgcgtcc
    30061 ctgccacgcc gagtacccaa tgatggtgca gcacgttttc agccagccat tgcgggccgg
    30121 aaatataagg cagcccacgg ttcaaaatcg cctcgaccac atccatcccg cgcctggcga
    30181 cattgccgat gacgtaaata tcggcttgaa attcttccaa ctgcgcggca tcgaagcctt
    30241 cgtgtacgcc tatgcccaag gcttcgagct gggtgctcat cggcggatac atcttcgcgt
    30301 cgcaaccgct gactttgaac ccggcttctt tggcaatggc ggcaatcccg cccataaacg
    30361 tgccgccgat accgataatg tggatgtgtt tcatcagagt tccctaatcg ttcaaattct
    30421 attgttcaac accgtaatgg ttgattcggt ttcaatcaat gcggtctgat gccggcatcc
    30481 gaaccgtctt tctgtgcatt ccctttcgga ttttggacgg cttcgtccct caccgtgtcc
    30541 gaataatcgc caactcctgc gtcttccgcc ctgtctttca cggcaggcct gaccgaactg
    30601 tacacccgat cccgccccaa acccgcgttg gtacaatctt ttcccggagt ttggctgtaa
    30661 ctggttttac cattatggcg gcactcgtaa acatttgccg aaaatacggt ctgaaccgct
    30721 gcaacaagca atatacttcc aatcatttta gatacgcgca tattaaactc ctttattgtt
    30781 cctcttcaaa aaaaggaatg tatttcaata gaacttcatc taattctttc attccttgct
    30841 cataattgaa cgggtattcc caaccatccc ctactttgat ttcagaaatg tctttaaact
    30901 tccgcttaag ggcggacact ttgtccatat cgtcatcaat ttcctgtttg ccgaacggta
    30961 ttttccgctt ttccaaaacc tcgtcataat aacaaggtaa attcagcaat agcccccaag
    31021 cgctatttgc gattacgact aatctatgtt tttccctagt taagtaatct gcaccgctat
    31081 catagattgc ccttgtcgga tcataaaaaa attcgcaatt tttcatagcg tccccacact
    31141 ttaaccgatt cgcaatttta tatccgcgat tttgcaagct gcccataaaa accgattgcc
    31201 tgccaagccg acactacttg ttaaagatgc ccgataatgc cgcaactctt ccgtatttac
    31261 cgacaaacgc cctatttgct tagagctgtt ttgttttcca caaaacttta atccgttgat
    31321 ttaacaaatt ttttaccaaa ttctttattt gttcggattc cattagcacg attttgagca
    31381 atatctgtca aaaccatttc caccatatcc aaaacaaaac cgccgccgcc acggcattgg
    31441 acgataagat gagtttccac tgcccgtcct tccgcaatgc cgccttcttg ggcttcaaag
    31501 cacccgcata aaaaaacggt gacagcagca tagaagcgat acataagaaa atcaatacgc
    31561 cgtaatagat tttttcagac ggcatttctc ttcctcaata cgccgcccgt tggcgcatct
    31621 gttcaaacag gcagattgtc gccgccatcg cgacatttaa agactcggtt gcatcgtgca
    31681 tcggtatcct gacacacttg tccgccctgt ctaaaactgc tttaccgaca cccgcacctt
    31741 cgttgccgaa cacccaagct gtcggttcgc acaaatcttc gccgtacaaa accgcctgct
    31801 tttcctcgcg caacgcggtg gcaaacacac ggtcttcata gtgcgccaac catatttcca
    31861 aatccgcctg cgaataaatg tccaacaaga aatgcgcgcc catacccgcg cgtaatacct
    31921 tgggcgacca cgcgtccgca caacccctgc ccaaaacgac cgtacctacc cctgccgccg
    31981 ccgcactccg taagacggtg ccgacattgc ccggatcttg cacgccgtcc aaaaccgcac
    32041 agtcgccttt gtccggcaag gttccgccca atgggatatc aatcagtgta agtatctcgt
    32101 ctgcacaact caagctgctg atttttttca atatgccgtc tgaaacggaa aaaatcccgt
    32161 cttccggcaa aaccgctgtc aatttaagga cttcttcaga cggcatttta gcttcgggaa
    32221 tatatacccc gaccggcttt ctgccggatt gcaggaaaac ctgaagcagg tgcacgccct
    32281 ccaaaacggt ttgggcatac tgccgtctga actttccttg cgacaacagg cggtgcaggt
    32341 gtctgatatg ttcattattg gtcgaactga tgtgtttcac ttcacaagcc ttttagaaat
    32401 atgcaaccgg acaatacccg actgaacgcc gccgaaatct gtttgcgata ttatcaaaat
    32461 aaaatgggaa agtcaggatg tacgggcaaa atgccgtctg aaaccgagat ttgaaaatcc
    32521 gcacaacagt ttgccaacgc caatggacgg tttccgctgt cggcaacgag cggccaaatt
    32581 gggcgcagga aaggcagaac ccgtttcccc tgaaggattt ttttcacatt tttatggatg
    32641 ccgccgacag ccaacgtatc cgatgccgct accgtcctca aaatcccgtc ctgttccaac
    32701 atggcttcag gaagtccgaa cggatgccgc ttcaacacaa atctatgttc tgtcagtatt
    32761 tttttgagat ttccgcttat cgtcccatct ttcagaaaag ccggttttgc caactgttcc
    32821 gacgcaaaca caaacagcct gcctgcataa tgatgcaatt caaagccttg caagttccaa
    32881 cgtccggttt ttgcctccgt caaaacgcgg gcaatgtcgg caagtgcgtt ctgattcggc
    32941 acggggatgc cgttttcctt tagaaaattc cgcaaaatat gggttttccg gcgcggggaa
    33001 aacgtcagcc accgcgccgt atcgaaataa ccggccccgc aaacccaacg gcagtcttga
    33061 acgacgacct cttccaacaa agccaaatct tcctgcaaag cgcggacatt gttcagcaca
    33121 tgccgcccga aatggggaat ctgcgccgaa agttcgggca aaatacggtg ccggaagcgg
    33181 tttcgcaaat aagccgtatc ggtattgctt tcatcctcga tattcggcaa accgtgtttt
    33241 cgggcataat cccatatgtc ttggcgcgaa aaaggcagca aaggccgcca gataatgccg
    33301 ttttccccca aagggcggac ggcgggcata gccgccaaag cgcgcaaacc tccgccgcgc
    33361 gcgactgcca gcataaaggt ttcgatttga tcgtccctgt ggtgcgccaa cgccaaaacg
    33421 tcaaagcctt tttcggcaaa ctcggcataa cgcttttgcc ttgccgccgc ctctatgccc
    33481 aaaccgtttt tttccacgca gaccttaacc gtttccagcc ccacccctag catatcgcaa
    33541 taattccggc agaaatccgc ccaatcgtcg gctcggggac tcaagccgtg atggatgtgc
    33601 aatgcctccg gcacaaatcc tgcccgcttc ccggcacaaa ccagcagatg cagcaaaacg
    33661 acggaatcca agccgccgct taatgccact gctgtttttt ttccattcag accttgagga
    33721 aaacaatcct tcaagcattg ctcaaacgca tctaaagtca gcacggcttc atcccgataa
    33781 agaaaaagca agcagtttca gacaaactgc ttgctgcttg ttctacccga attattgtta
    33841 cgaaaatttg ccgtaagcca taatgcggtc gaaacggcgc gaaagcaaat cggcaagcgg
    33901 gatgctttgc gcttcgtgca gttgtttttc caaaacggct tttacgtttt tcatggtttg
    33961 cccgaaatcc ctatgcgcac cacccaaagg ctctttaatg acggtatcga ccaagtccag
    34021 cttttgcagg cggtcggcag taatacccaa agcctgtgcc gcatcagccg ccttttcggc
    34081 ggttttccac aaaatcgaag cacagccttc gggggaaata acggaatagg tcgaatattg
    34141 cagcatattg acgtaatcgc ctacggcgac cgccaacgca ccgcccgaac cgccttcgcc
    34201 gatgacggta cacaaaacgg gaacgcgcag tcgggtcagt tcgtacaggt ttttgccgat
    34261 ggcttccgac tgcccgcgtt cttccgcgcc gatgccggga tacgcgcccg gcgtatcgat
    34321 aaaggtcatg acgggcaagc cgaatttttc cgccgttttc atcaggcgca gggctttgcg
    34381 gtagccttca ggacggggca taccgaagtt gcggcggatt ttttctttgg tgtcgcgccc
    34441 tttctgatgc ccgacgacca caacgctttg tccgttgaaa cgcgccaatc cgccgacaat
    34501 cgcatgatcg tcggcaaagt ggcggtcgcc gtgcagctct tcaaaatcgg taaacagcgc
    34561 gtcaatgtaa tccaaagtat agggacgctg cggatgccgg gaaacctgtg aaatttgagc
    34621 gggtgtgagt ttgctgaaaa tcgatttggt caaatcgttg ctttttttct gcaaacggtg
    34681 tatttcgtcc gaaatatcga cggcagactc gtcttggacg aaacgcagct catcgatttt
    34741 gttggtcagt tcggctatgg gttgttcaaa atccaaaaaa acaggtttca taaaatgaag
    34801 ctctcatcaa cagattctgc cgctatcata cgctatttgt gcatattccg cagttaattt
    34861 attgtccaaa acggcttttc cacaccctct ttttgtgttc ggacagcatg gaaaacagcg
    34921 gattagtgca tatatactag ttataaggcg atgtatctta tgctaaactc gaaacaaatt
    34981 caattatttc aaacaaaatc gtatgaaaga caagcacgat tcttccgcca tgcggctgga
    35041 caaatggctt tgggcggcac gttttttcaa gacccgttcc cttgcgcaaa agcatatcga
    35101 actgggtagg gttcaagtaa acggctcgaa agtcaaaaac agtaaaacca tagacatcgg
    35161 cgatattatc gacctgacgc tcaattccct tccccataaa atcaaggtta aaggtttgaa
    35221 ccaccaacgc cgcccggcac ccgaggcgcg gcttctgtat gaagaagacg cgaaaacggc
    35281 agcattgagg gaagagtgca aacagctcga ccaattcagc cgcatcactt ccgcccatcc
    35341 cgacggcaga ccgaccaagc gcgaccgccg ccaactggac aggctgaaaa aaggagactg
    35401 gtaaaaccat tcccgctgca aacagaagca cgaaccgcgt accgccgcat ttcagacggc
    35461 attcctgaaa cttattccgc ctcgtctatc catgcctgct gcacggcttc aaggatgcgt
    35521 tcgccgcaac gggcgggatc gtcgtcaaat tcgggcaatg ccataatcag gtcgcgcagt
    35581 tgggtaaagc gcacggtttt gggatcgatg gcttcgccgt gcaggtcgta gagttcttcg
    35641 gcgatgcgcc gggtatcggt ccatttcata taaatttcct tttatcgtcc ggcattgaag
    35701 ccgtgtagaa tatgtttcgg caaaactgcc gccccttgag gatggctgaa tgtttcaact
    35761 cgccggacgg cacatcaagc accaccttat gtgtcgtcct gatccgggag gagttacccc
    35821 tcccaaacaa atctgattct accgccctga agagcggggt ttcaaccgat aaggaagatt
    35881 gatgaacaat atgtttgccg caaaattgtc cgaactggtt tataccgctt ccgaccatcc
    35941 cgactccctg tcggagatgg aggagtttga ccgcctgatt ctgctgatac gcaaactgta
    36001 tcaaatattg gacgggcaac ataccctcta cagagtaacg gtttgcctta actaccggag
    36061 caggcgcggt ctgattgctt tggataaagc ggctgtcggt tgcgatgcgg caatgttgcg
    36121 cgcccggcgt tggcgcatct gcatgcagat tgcggaacgc ctttccgagt cggtcgggcg
    36181 gcttcacggt tgcggcagaa agcgtgtccg ccgcctgccc cgagcttgaa ggacgctatc
    36241 tcgaccttgt ccgccgcgcc gccgtctctt tcggtttcgc gcaaggacag gcaaatccgg
    36301 caggcgggca ggaagaaacg caagccgcct cagatgccgt atcgggtcgt tccgtcaaaa
    36361 aaccgataaa aatcaatata gaatcgaaag ttccgtcttc acaaacgtct gtttttcggt
    36421 aattgcgcct tatccgcgca acaatgccgt ctgaagcgtg ccggcttcag acggcctgtt
    36481 ttcagtgttc ttcgcgcgcg tggttgatgg tgtatttggg gatttccaca atcaaatcct
    36541 cgccggcaac aaccgcctga caactcaggc gcgaatcggc ttccaaaccc caagcctgat
    36601 cgagcaggtc ttcttccaat tcggccggct cttccaaact gtcgaaacct ttgcggataa
    36661 tcacgtggca ggtggtgcag gcgcaggatt tttcgcaggc gtgatcgact tcgatgtcgt
    36721 ggtcgagcag cacgtcgagg acggttttgc cttcgggcgc gttatcgatg actgcaccct
    36781 cggggcataa tgttgcgtgt ggaagtacgg tgattttggg catttttatt gtctttctgt
    36841 aaaaagtatt tgtgtccgtt tttaggaaaa ctgtctgcct aatgtcgcgg ttcgagcagg
    36901 tcggcaacat tttgccagcc cgtcaggcgg gcaaaatcga gggcggtata gccttccgat
    36961 gtctttgagg caggatttgc gccgtattca agcatggctt gaaccataag tgcatttcct
    37021 gccatggctt catgatgaag cggcgtatag ccgtcttcgt cctgctcatg cagaaaatcc
    37081 ggattttggg acaactgttg ccgaaaatcg tcaatacagt tccggcacat cggatgctct
    37141 tccaaatttt cgggatgttc tttttcttca tacaccggca atacctgccc gggatcgccg
    37201 aaatcgattc cccaggcggt atcgtgttcg gtacgctcct cttccgtcat ctgtccgcgc
    37261 attgcctgta cggtaaagcc gccgtagggg atgccgttgc acacgaacat ccagtcgctg
    37321 atgtcgccaa ccggaacgca aacactttcg ccttgttcga cattgatcag tccgccgggt
    37381 tcattgttca gcacaccgta aatgtaaaga ccgtcaaaat aaatatcgtc gatccacata
    37441 tgttcgcaaa tttcgccgtc ttggaaaaaa gggactttga ccatggcaaa atccaaggcg
    37501 gaaataatgc ggcggcgttc ccaaaaaagc tcgcgccaaa agtatttgaa tgttttacgg
    37561 gcgcgttcgc cggcacggtt taccggttcg tctgcctgtt ctacataata aatgacggaa
    37621 tcgcccatca tactgctcct caacgtgtac ggtatctgtt tgcaccttac cgcggttttc
    37681 tgcctccggc atccgattcg gatttgaaaa gttccaaata ttcggaatag gctttttcta
    37741 agtttacccg cgaaaaccag gtgtaaatat ggatgccgtc tgcaatttcg tcggtcagcc
    37801 tcctgccgct tttgcgggaa tatgcttctt taaaaaggcg gattttcgat tttttcggct
    37861 tgtaggatgc cgcgtccgcc aaaagccggg gcagcgcgta agggtattcc cggtgtccgc
    37921 gccgtaccaa atcaagcagt tgtttgcggc cggacccggc attcccttcc tgccggcgtt
    37981 cgtggacgct tttttcaaat tccgattccg ccgtttcgtt tttcgggaac acgatttcgc
    38041 cgcgaagcga taaaaacaaa cgtttcacaa ggttttcgtc cacggcgaac aattcggtat
    38101 cgcctatcct gcttttgctg aaaatttcat ggagcaggtt ttttttcttt gtaattgtcg
    38161 gttttgacgg cgaggaggcg ttccaacccg gcaacgttcg catagccgtt gttttccaaa
    38221 tggcgcattc tgccttcaaa attgcttacg tccgaaatgc cgattttata tagtgaatta
    38281 acaaaaatca ggacaaggcg gcgagccgca gacagtacaa atagtacggc aaggcgagcc
    38341 aacgccgtac cggtttaaat ttaattcact atacacgccc ttgatgacgg ttttcatcga
    38401 atagacaatg cctgactttt ccatatcgat gtttttcaag tgttttcgat ccttcagacg
    38461 gcatcaggtt atatctatgc cgtctgaaac cgtttaagta tcaaatatta tcgacactct
    38521 gacctgtcag cgcacgttgg atgttgcggt tcatgcgctt ggcggcgaaa ttgtcggtgc
    38581 tgcggctgag tttggcgaca gctgcacgga tgtcttcagc tttaccgtct ttcagacggc
    38641 cttgcaaatc ggcgatgtct cgctggattt gtgcaaactc ttcggcatcc agcaaatcgc
    38701 tgtccaactc gagggcggcg ttgacggcat cggtcaggct ttcggcttcg accacggctt
    38761 cggcgcgggc gcgggccgcc atatcttcgg cggcgttgcc catgctgtct ttgagcattt
    38821 gggtgatggt gtcatcgtcc aaaccgtagg agggtttgac ttcgatttgc gcctgtacgc
    38881 cggtgctttg ttcttgggcg gaaacggaca gcaaaccgtc ggcatcgatt tggaaggtaa
    38941 cgcgaatacg cgccgcaccc gccgccatag gcggaatgcc gcgcagggta aatttggcaa
    39001 ggctgcggca gtcggaaacg agctcgcgct cgccttgcac gacgtgtatc gtcatcgcgg
    39061 tctgaccgtc tttgaaggtg gtaaagtcct gcgcgcgcgc ggtggggatg gtggaattgc
    39121 gcgggatgat tttttccgcc aagccgccgt aggtttccaa accgagcgac aagggcgtaa
    39181 cgtccagcag cagccattcg ccgtcggctt tgttgcctgc gaggacgttt gcctgtatgg
    39241 ctgcgccgag cgcgacgact tcgtcgggat tgaggttgtt cagcggggtt tgtccgaaaa
    39301 aggtggcgac tgcctgttgg acgtgcggca tacgggtcga accgccgacc ataaccacgc
    39361 ctttgacttc gtttttaccg acaccggcat ctttcaacgc ctgtttgacc ggctcgagcg
    39421 ttttcatcac caaatgctgc gtcaggttgt ggaactcggc gcgactgatg cttgtgtcga
    39481 ttgccatacc gtctgaaagc gtcgcctgaa tgcgcgcttc ggtttgcgtg gtcaattgtt
    39541 ctttggcggc gcggacgagc gagagcagga gttggctgtc ttgttcgttg agttgggaga
    39601 gtctgttttg ttcgagcagg tagcagaaca aacgatggtc gaagtcatcg ccgcccaacg
    39661 cgctgttgcc gccggtggct ttgacttcaa acagtccttt ggtcagttgc aatacggata
    39721 cgtcgaatgt gccgccccct aagtcgtaca cgacaaacgt gccttccgag gcgttgtcca
    39781 gcccgtaggc gattgcggcg gcggtaggtt cgttgagcag gcgcaatacg ttcaaaccgg
    39841 ccagacgcgc ggcatctttg gtggcctggc gttgcgcgtc gtcgaaatag gcggggacgg
    39901 taatcaccac gccgaccaaa tcgccgccca aggtttcttc ggcacgcaat ttgagggttt
    39961 tgaggatttc cgccgacact tcgacaggcg ttttcacccc ctgccgcgta tgcagttcga
    40021 taaagcgttg attgtcgccg aaacggtaag gcaggtaatg cgtattttga tgcagatcgg
    40081 caagagtccg cccgataagg cgtttggcgg agctgacggt gttcagcggg tcggtttttt
    40141 gggcggacag ggcggttttg ccgacttcaa tgccgccgtt ttccagatag cggacgacgg
    40201 aaggcagggt aacgcgcccg tcggcatcgg gcaggcaggc ggcactgccg ctgcggacgg
    40261 tggcgaccaa gctgttggtc gtacccaaat cgatgcctgc cgccaaacgg tgccggtgcg
    40321 gggcggcgga catacccggt tctgaaatct gcaaaagagc cataaattct gcctgtcggt
    40381 gcggtgtgta aaggaaggaa ggcggcattt taacagaatt tgcgccgata gttgagcaaa
    40441 ccgctaagga ttgggccgtg ccgccgaaat tgccctctac cgccaaatac cgataaaatc
    40501 cgcctttccc gcccatcatt aggaatataa atcgtgaaag gactcgacta ttgccgccaa
    40561 aaagcagagg aaagccgctc cagttttttg tcgggcttcc gtttcctgac tcaggaaaaa
    40621 caggatgcgg taacggttct gtatgccttt tgccgcgaat tggacgatgt ggttgacgaa
    40681 tgttccaacc ccgatgttgc acaggcaaca ttgaactggt ggcgcggcga tttggacaat
    40741 gcgttcggcg gcgcgatgcc ggaacacccc gtcaatcagg ccttgcggca ggttaaggaa
    40801 accttcaagc tgccgaaata tgaactggaa gccttaatcg acgggatgca gatggatttg
    40861 gttcaggcgc gttacggcag ttttgaggaa ttgaaactgt attgccgccg cgtcgcgggc
    40921 gtggtcggct gcctgattgc gcggattttg gggttttcag acggcaaaac gctggaatac
    40981 gcggataaaa tgggtcttgc gctgcaactg accaacatca tccgcgatgt cggcgaagat
    41041 gcgcgcaggg gacggattta cctgccgatg gaggaaatgc agcggtttga cgtacccgca
    41101 agcgtgattt tgcaatgcag cccgacgggc aattttgccg aattgatggc gttccaaatc
    41161 aaacgcgccc gtgaaaccta ccgcgaagcc gtatcgctgc tgcctgatgc cgataaaaaa
    41221 gcccaaaaag tcggactggt tatggcggcg gtttattacg cgctgttgaa cgaaatcgac
    41281 cgagacggcg cgcaaaacgt cctcaaatac aaaatcgccc tcccttcgcc gcgcaaaaaa
    41341 cgcattgccc tgaaaacctg gttattcgga ttcaaaccgc gccccggcac gccggaacgg
    41401 gcataaggcg catactgccc ttcaggaaaa cccaaccctc gccgccccga aaaacggggt
    41461 ttcaaccatt ggaggaataa atgatgaaca cgccgcatcc gcgcccgaaa atcgccgtca
    41521 tcggcgcagg ctgggccggc ttgtccgccg ccgtcacctt ggcgcggcac gccgacgtta
    41581 ccctgtttga agccggccgg caggcgggcg gaagggcgcg cacactggcc ggaaataccg
    41641 acggtttcgg ttttttggac aacgggcagc acattttgct cggcgcatac cggggcgtgt
    41701 tgcgcctgat gaaaaccatc ggttcagacc cccgtgccgc ctttttgcgc gtaccgctgc
    41761 actggcatat gcacggcggt ttgcagttcc gcgccctccc cctgcccgcg ccgctgcata
    41821 ttttgggcgg cgtgctgctt gcccggcgcg taccgtccgc attcaaagcc aaactgcttg
    41881 ccgatatgtc cgatttgcag aagtccgcac gcctcggaca gcccgacacg acagttgcac
    41941 aatggctgaa acagcggaac gtgccgcgtg ccgccgtaat gcagttttgg cagcccttgg
    42001 tctggggcgc gctcaacacg cctttggaaa ccgcaagcct gcgcgtgttg tgcaacgttt
    42061 tgtccgacgg cgtgctgacg aaaaaatccg gcagcgacta tctcctaccc aaacaggatt
    42121 tgggcgcaat cgtcgccgaa cccgccttgg cggagcttca acggctcggc gcggacatcc
    42181 gcctcgaaac gcgcgtatgc cgtctgaaca ccctcccgga cggaaaagtc ctcgtcaacg
    42241 gcgaagcctt cgatgccgcc atacttgcca ccgcgcccta ccacgccgcc gcgctcctgc
    42301 ccgaaggcac gcccgaacac gttcagacgg catatcaaaa ccttcgctac cacgccatca
    42361 ccaccgtcta tctgcgctac gccgaacccg tccgcctgcc cgccccgctg accggcattg
    42421 ccgacggcac ggcacaatgg ctgctttgcc ggggcaggct cggactgccc gaaaacgaag
    42481 tctccgccgt cattagcgtt tccgaccgcg tcggcgcgtt tgcaaaccgg gcgtgggcgg
    42541 acaaagccca cgccgacctc aaacgcatcc ttccgcattt gggcgaaccc gaagccgtgc
    42601 gcgtcatcac cgaaaaacgc gccacaaccg caaccgatgc cccgccgccg gatttgtcgt
    42661 ggctgcaccg gcaccgtatc ttccccgccg gcgactacct ccacccgcac taccccgcca
    42721 cgctcgaagc cgccgtacaa tcaggtttcg cgtcggcgga agcctgcctg caaagcctga
    42781 acgatgccgt ctgaaaacgc cggccggcat cgggacgctt tacaaggtgc ggcaaaacgc
    42841 taaaatacat taaccgtcaa acccaaagga caaccatgcc gacactgacc gacaaaacca
    42901 tcttagttac cggcgcatcg caaggcttgg gcgaacaggt cgccaaagcc tacgccgccg
    42961 aaggggcgac cgtaattctg gtggcacgcc atcagaaaaa actggaaaaa gcctatgacg
    43021 cgattgtcga agccggacac cccgaaccct tcgccatccg tttcgacctg atgagtgcgg
    43081 aagaaaaaga attcgaacgg tttgccgcca ccattgccga agccacgcaa ggcaaactgg
    43141 acggcatcgt ccactgcgcc ggctattttt acgccctctc gccgctggat ttccaaaccg
    43201 tcgccgaatg ggtcaaccaa taccgcatca acaccgtcgc cccgatgggg ctgacccgcg
    43261 ccctgttccc gctgctgaag cagtcgcccg acgcgtccgt catcttcgtc ggcgaaagcc
    43321 acggcgaaac acccaaagcc tactggggcg gcttcggcgc gtccaaagcc gcgttgaact
    43381 acctgtgcaa agtcgccgcc gatgaatggg aacgcttcgg caacctgcgc gccaacgtcc
    43441 tcgtccccgg ccccatcaat tccccgcaac gcatcaaatc ccatccgggc gaagccggaa
    43501 gcgaacgcaa aagctacggg gacgtactgc ccgcatttgt ctggtgggca agtgccgaaa
    43561 gcaaagggcg gagcggcgaa atcgtttacc tctaaatccg gaaaaggcag gcatcccgcc
    43621 cgcgccgcgc ttccgttttg aaaaatatcc gtaccttccg cgcgcatccg tcccgcacag
    43681 acaaacacct tgacttaaat ttacataagg acaataatga taacccgtta tttccctaac
    43741 tggaccttag ggtaaatttt atgaaaaccg tttccgccgc catcgctttt gccgccgctg
    43801 ccgtttcact gaccggctgt gcgaccgagt cctcacgcag cctcgaggtt gcaaaagtcg
    43861 cctcctgcaa tacgcaatat cacggtgttc gcaccccgat ttccgtcgga acattcgaca
    43921 accgctccag cttccaaaaa ggcattttct ccgacagtga agaccgtctg ggcagccagg
    43981 caaaaaccat cctggtaaca cacctgcaac aaaccaaccg cttcaacgta ctgaaccgca
    44041 ccaaccttag cgcattgaaa caggaatccg gcatttccgg caaagcgcag aacctgaaag
    44101 gcgcagatta tatcgttacc ggcgatgtaa ccgaattcgg acgcagagat gtcggcgatc
    44161 atcagctctt cggcattttg ggtcgcggca aatcgcaaat cgcctatgca aaagtggctc
    44221 tgaatatcgt caacgtcaat acttccgaaa tcgtctattc cacacagggc gcgggcgaat
    44281 acgcactttc caaccgcgaa atcatcggtt tcggcggcac ttccggctac gatgcgactt
    44341 tgaacggcaa agttttagac ttggcaatcc gcgaagccgt cgacaacttg gttcaggctg
    44401 tcgacaacgg cgcatggcaa tccaaccgtt aaggagaaat gatgaatccg aaaaccttgg
    44461 gccgtttgtc gctgtgtgcg gcggtcttgg ctctgaccgc ctgccggcgg cgggcataaa
    44521 aacctgtatt attacggcgg ttatcccgat accgtctatg aaggtttgaa aaacgacgac
    44581 acttcgttgg gcaagcagac cgaaaagatg gaaaaatact ttgcggaagc cgccaacaaa
    44641 aaaaatgaat gccgccccgg gtgcgcacgc ccatttggga ctgctgcttt cccgttcggg
    44701 agacaaagag ggcgcgttcc gccaatttga agaagagaaa aggctgtttc ccgaatcggg
    44761 cgtatttatg gacttcctga tgaaaaccgg taaaggaggc aagcgatgaa acctttgatt
    44821 ttagggcttg ccgccgtgtt ggctctgtct gcctgccaag ttcgaaaagc tcccgacctc
    44881 gactacacgt cattcaaaga aagcaaaccg gcttcaattt tggtggttcc gccgctgaac
    44941 gagtcgcctg atgtcaacgg cacttggggg atgctggctt cgaccgccgc gccgatttcc
    45001 gaagccggct attacgtctt tcccgccgca gtcgtggagg aaaccttcaa agaaaacggc
    45061 ttgaccaatg ccgccgatat tcacgccgtc cggccggaaa aactgcatca aattttcggc
    45121 aatgatgcgg ttttgtacat tacggttacc gaatacggca cttcatatca aattttagac
    45181 agcgtgacga ccgtatccgc caaagcacgg ctggtcgatt cccgcaacgg gaaagagttg
    45241 tggtcgggtt cggccagcat ccgcgaaggc agcaacaaca gcaacagcgg cctgttgggg
    45301 gctttggtcg gcgcagtggt caatcagatt gccaacagcc tgaccgaccg cggttatcag
    45361 gtttccaaaa ccgccgcata caacctgctg tcgccctatt cccgcaacgg tatcttgaaa
    45421 ggtccgagat tcgtcgaaga gcagcccaaa taagcatatg ccgtctgaac ctctccgagc
    45481 ttcagacggc atatgcgaat cttgatactt gcaagccgca ccgcccaaaa tcaggcgacc
    45541 ggtttcaata tccgaacaag gttcgggttc tgtattttgc agaacccgaa ccttttattc
    45601 aaatttaaac ttaattccgg cgtggttgta tggtaaatta tagtggatta aatttaaacc
    45661 agtacggcgt tgcctcgcct tgccgtacta tctgtactgt ctgcggcttc gtcgccttgt
    45721 cctgattttt gttaatccac tataacaagc aatatgccct gaccggcaaa acttccccaa
    45781 acttcatcaa aaaatcaact gtacccgtct ttttcagacg gcattgatac cgtaagagat
    45841 ttcccatgca aaaccggcaa accgagcttt attccacgcc gtcttggctg ttgcagacct
    45901 tattgatgat taccgccgcc tcggcggtga ttctgttttt cgcgcgcaac acgcgcttgg
    45961 ggcgggagtt tgcctatatc ctgcgccttt gcctgacacc gaaaagcacg gtcaaagtat
    46021 tgctgctgat tacggcaatg gttacgctgc tgttaaccga agtgcggttg aatgtgttga
    46081 gtaccttcat gtccaaagga ctttacgatt cgatgcagga tttgaatgct tcggcatttt
    46141 ggatgtttgc agcgatgaac gccggcgtgg tactgatacg ggcgtttaac aacgtcgtta
    46201 acgactttct cgattaaggc ttggcgatta aatggtcgga acggctcaat gaagttttga
    46261 cttcgcgctg gcttgccgac aaaaactact accgcctgca aatgcgccgc cacgcgccgg
    46321 acaacatcga ccagcgtatc caacaggatg cgcaggaatt catcgcttcg accatcgaat
    46381 ttgtgcgcgg catggtcaat tcggtcgtta cttctctgga atttgccgtt gttttgtggg
    46441 gcttggcagg catcctgacc gtgttcggct tcgacattcc gcacggcatt gtttggtttg
    46501 tctttatttt catgattttg gcgatcttta tcgccatgtg gataggcaac cctttgattc
    46561 gttacaatta tgaaaacaaa aaactcaacg gcgactaccg ttattccctc atcctcgtac
    46621 gcgaccacgc cgaaagcgtg gcgttttaca gcggcgaaca acacgaacac ggccggcttt
    46681 ccgaccgctt taaagccatt atccgcaacc gttggcgcat cgcgcggcaa agcgtctgct
    46741 taagcggctt taacgatatg ttcaccaacg gcatcaaact cttcctgatt attttgcaag
    46801 ctccgcgcct gtttgccgga caaatcaaaa tcggcgacat ccagcagacc gtccaagcct
    46861 tcgcgcgact gcaaaacgcg ctgtctttct tccgaatgtt ctacaacaaa ttcaccgcct
    46921 gccacgcccg actggagcgt ctgtacggct ttttgctgag taccgaagaa caacacggcg
    46981 cgcagcaacc cgagattagc gaagtttcag acggcattgc actggaaaac gtcgccctgt
    47041 tccgccacaa cggcgaagtt ttattggacg gcatcaacat caaactcaaa agcggcgatt
    47101 ccctgctgat acgcggcccg agcggttgag gcaaaacctc gctgctgcgc gcgctggcgg
    47161 ggctttggcc gttcggcagc agcggcaaag tcagccgtcc gccgcatcaa ggcatcctct
    47221 tcctgccgca acgcccgtac acggcacaag gcagcctgcg cgacgcggtt tgttaccccg
    47281 atatcgacaa acagcatccc gagttagccg aagccatgaa cacctgccgc ttgggttatc
    47341 tgattgacaa attggataaa acagacggct ggcagcacaa actctccccc ggcgaactgc
    47401 aacgcgtcgc cttcgtccgc gccctgctct ccaagcccaa aatcgtcctg ctcgacgaag
    47461 ccgccgccgc cttggacgaa ccggccgaag cctagctcta ccgcgcctta aagcaaaaac
    47521 tgcccgacag catcatcagt atcggacacc gaagtacgct caacgcgttc cgcaatatgc
    47581 acctcgatgt cggcaacgct gcctgcggct gaacgatgcc gtctgaagcc gttttcagac
    47641 ggcatcgttc aaattggaaa gacacgcgaa aacaacgtac aataaacccg attccaaccc
    47701 atccggtatg cctttgtgaa ccagcttatt ttcgactttg ccgcacacga ctatccgagt
    47761 ttcgacaaat tcctcggcac ggaaaatgcc gaactggtct atgtgttaca gcataaacac
    47821 gacccgttta tctacgtctg gggcgaagaa ggcgcgggca aaagccatct gttgcaggcg
    47881 tgggtcgcac aggcgctcga agccggcaaa aacgccgcct atatcgatgc cgcctccatg
    47941 ccgctgaccg atgccgcgtt tgaggcagaa tacctcgccg tcgatcaagt cgaaaaactg
    48001 ggcaacgaag aacaagccct gcttttttcc atcttcaacc gcttccgcaa cagcggcaaa
    48061 ggctttttgc tgctcggttc ggaatacacg ccccagcagc tcgtcatccg cgaagatttg
    48121 cggacgcgta tggcatactg cctcgtttac gaagtcaagc cactaaccga ccaagaaaaa
    48181 atcgacgcgc tcgccaatat ggcggcggca cgccaagtaa ccgtcgattc cgaaattttc
    48241 gaatacctgc tcaaacactg gcggcgcgat atggacagcc tgatgatgat gctcgacacg
    48301 ctggacaact acgccgtaac catgggcaaa cgcatcaccc tgccgctgct tcgccagctt
    48361 ttgaaacaac aggaaaccca atgaaaaacc tcgccatctt cgacctcgac aacaccctca
    48421 tcaacaccga ttccgaccac gcctggccgc aatacctcat caaaaaaggg ttggttgacg
    48481 ctgccgaaac cgaagcgcaa aacgaaaaat tctaccgcga ctaccaaaac ggctgcctcg
    48541 acatcgacgc attcctcaaa ctccacctcg ccccgctcgc ccgttacagc aaagaagagc
    48601 tggcggagtt tcaccgcgaa tttatggcgg aatacatcat cccccacatc tcgcctatgc
    48661 agcgtatgct ggtgcagagc caccaaatgg caggcgacga aaccctcgtg atttcctcaa
    48721 ccaacgagtt catcatcacc cccgtctgcc gccttttcgg catcgccaac atgatcggca
    48781 cacaactcga aaccggtccc gacggccgct acaccggcaa ttacatcggc acgcccagcc
    48841 tcaaagaagg caaaatcacc cgtctgaacc aatggctcgc cgaacgcggc gaaacgctcg
    48901 aaagctacgg caaaacctat ttttacagcg actccaaaaa cgacctgccg ctgctgcgcc
    48961 tcgtcagcga acccgtcgcc gtcaaccccg atgccgaact ggaaaaagaa gccaaagaaa
    49021 aaggctggcc ggttttgaat ttcaaatgac gcaatgccgt ctgaagcccg attcggcgtt
    49081 cagacggcat tgaggctgaa atcccaaaaa cccaaagcca caggaattca tccggaaaaa
    49141 caaaaaacac aggaattcat cgggaaaaac aacaatcttt ccaccgtcat tcccacgaaa
    49201 gtgggaatct tgatccgtcc gcacggaaac ttatatttcg tcattccacc aatcccacac
    49261 cacccttacc ggttcaaatc cgcacaaaaa tccacattcc gtcattccca cgaaagtggg
    49321 aatctaggac gtaaaatcta aagaaacctt tttttcccga taagtttccg tgccgacagg
    49381 tctggattcc cgcctgcgtg ggaatgacga aatttcaaag ttatggcgtt atcggaaaaa
    49441 cagaaaatca aagccggaga atttatccaa aacaaccgga tttcaaaaaa accaaattcc
    49501 cggcgggaat gacggatttt aggtttctgt tgtggtttct gtttgctcgg gaataacaac
    49561 ttagaaattg cccgaaaccc aaaaagcaga aaccggcttc ccgccaaagc cggcattttc
    49621 cgactttgct tcaggcatct tcccctctgc ctaaaatcat cgacccgata ccggcatcgg
    49681 taaagatttc cagcaaaagc gcgttgggca acctgccgtc gatgatgtgc gtggctttca
    49741 caccgttggc ggcggcttcg accgcagaag cgattttcgg cagcataccg ccatacagcg
    49801 tgccgtcggc aatcagccca tcaatccgtt tcggcgtgag tttggtcagc agattgcccg
    49861 ttttgtccat cacaccggcg atattcgtca tcatcaagag tttttcggcg ttcaattctt
    49921 ccgccaattt gcctgccacc aaatcggcgt tgatgttgaa cgcttcgcct tttttaccta
    49981 cgccgacggg ggcgacgacg ggaatgcagc cgcgttctat cagcccttta accaaaccgg
    50041 tatcgatgct ttccaccgta ccgacctgtc cgatgtccac gctattctgt tcgggcgtat
    50101 cgaccaaaag tttcttcgcc ttaatgaaat ggtcgtcgcg cccgctcacg ccgaccgcgt
    50161 gccctccata tgtgttaatc atcgacacga tttccttgtt gacgtgcccg cccaatacca
    50221 tttcgacaat atccatcgtc tctttgtcgg taacgcgcat tccttggaca aattcgccct
    50281 ttttgccgac tttttcaagc atcgcattga tctgcggtcc gccgccgtga acgatgacgg
    50341 gatgaatgcc gaccagcttc agcagcacga cattgcgggc aaacccttct ttcaaggcag
    50401 gttcggtcat cgcgttgccg ccatacttga tgacggcgac cgaaccggaa aaccggcgga
    50461 tgtaaggcag tgcttcggca aggatacgcg ccttgtcggc ggcggaaata atgttttcaa
    50521 actccataac ggctccatat tgttgacaat ttcggcttat gttaacgcca aatgccgtct
    50581 gaaacaatct tcagacggcg tttggcacac cttcgggcag gttgaagcct tgcgatacgg
    50641 gcggtaaaat cgcggtttat ccggttcgag aaaccggcga cagcaaggac tttcccatga
    50701 ttaaaatcag cacccgcttc gatgccggct cggtcgtcgt caaagacctg accgaccctt
    50761 ccaacatccg cctcgccctg cgtcccgaca acgcctccga tttcgcacaa tggttctact
    50821 tccggctgca aggcgcggcc tatcaaaact gcatcatgca ttttgaaaac gcggcagaag
    50881 ccgcctaccc gaaaggctgg gaaggctatc aggcgtgtgc ctcatacgac cgccgcaact
    50941 ggttccgcgt accgacttcc tacgaaaacg gcgtactgac cgtcaatcat acgccgctgt
    51001 ccaacagcgt gtattacgcc tatttcgaac cctattccga agagcagcac ctcaacctcc
    51061 tcggcgacgc gcaaggcagc ggcctgtgcc gcatcgacga cttgggcagc accgtgcaag
    51121 gccgcgacat caatctgctg accataggca accaagtcga aagcgacatg aaaatctgga
    51181 ttaccgcccg ccagcacccg ggagaaacca tggcggaatg gtttgtcgaa gggctgctcg
    51241 gcaggctgct cgattcgcaa gacccgacag cacgcaccct gctcgaccgc gccacgttct
    51301 acatcgtccc caatatgaat cccgacggtt cggcactggg caaccttcgt accaacgccg
    51361 ccggtgcgaa cctcaaccgc gaatgggaaa acccgacttt ggaaaaaagc cccgaagtgt
    51421 tcttcgtgcg cggaaaaatg ctggaaaccg gcgtggattt gtttttggac atccacggcg
    51481 atgaaggtct gccctttgtc tttgttgcag gaacggaagg tgtgccgaac tacaatccgc
    51541 gcatttccgc gttggaagcg cagttcaaaa ccgccctttt gaacgccagc cccgatttcc
    51601 aagacgaata cggttacgaa aaagacgcgc cgggcaaagc gaatatgacc ttggcgacca
    51661 actgggtcgg caaccgtttc aactgccttg cctacacgct ggaaatgccg ttcaaagaca
    51721 acgccaacct gcccgacgac gacttcggct ggaacggcca gcgttctttg cggcttgggg
    51781 aagcggtgct ttccgccatt ttgaacgtcg ccggcgattt gcgctgacac tccccactga
    51841 aaatgccgtc tgcaagccct tttgcgtctt tcagacggca tttgaaaacg aaagcccgtt
    51901 atgattatcc atcaccaatt cgatcccgtc ctcatcagta tcggcccgct tgccgtccgc
    51961 tggtatgcct taagctacat cctcggattt attcttttta cctttctcgg cagaaggcgc
    52021 atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg acgacttcct gacatggggc
    52081 attttgggcg tgattttggg cggacgcttg ggctatgtcc tgttttacaa attctccgac
    52141 tacctcgccc atccgcttga tattttcaag gtatgggaag gcggaatgtc gttccacggc
    52201 ggctttttgg gtgtagttat tgccatatgg ttgttcagcc gcaagcacgg catcggcttc
    52261 ctcaaactga tggacacggt cgcgccgctc gttccgctgg gtctcgcttc gggacgtatc
    52321 ggcaacttta tcaacggcga actttgggga cgcattaccg acattaacgc attttgggca
    52381 atgggcttcc cgcaagcgca ttacgaagat gccgaagccg ccgcgcacaa tccgctttgg
    52441 gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc cctcgcagct ttatcagttt
    52501 gcccttgaag gcatctgcct gttcgccgtc gtttggctgt tttccaaaaa accgcgcccg
    52561 accgggcaga ctgccgcgct ttttctcggc ggctacggcg tgttccgctt tattgccgaa
    52621 tttgcgcgcc aacccgacga ctatctcggg ctgctgacct tggggctgtc gatggggcaa
    52681 tggttgagcg tcccgatgat tgttttgggt atcgtcggct ttgtccggtt cggcatgaaa
    52741 aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca tggaaatccg gtttcagacg
    52801 gcatttttac gtttggttca gatgaaagca aacgcttcaa ttcttaccgc aacacgcctt
    52861 gtatttcctg ccgctgccgc acggacaggg atcgttcctg ccggtttttt ccccttccct
    52921 gcggacggtt tgcggtttgt tgatgaccgc ctgccagtag cggtagatgt ctgccagcgc
    52981 gtaaggcagt tcggacgcaa gttccgccag ctcgccttcg gtgaattgca ggcggatagc
    53041 gccgttttcc tcttcgtcgt aaatgccgcc caatgccatc acggggtaaa acagctcttc
    53101 aaacgcttca tcatcggcgg cttcaaacca atcggtcggc acaatgtcca aaccgtaaag
    53161 ataggcgttg caccaagtgt aaaaatcgct gccgccctcg ccgtcgtcgt agagccacaa
    53221 atcgggcagc tttttatccg acatcgcggc ggttgtttcc atcgccattg ccaaaaccag
    53281 ccgttcgatt tcggaacgtt cggcggcggt aaattgcgat tcgtcgccca acacttcggg
    53341 cagccagtcg agcggtgcca atttgtccgg cccgctcaac agcgccgtca taaaaccttg
    53401 aacctcgtcg caacgcatcg tgttgccttg ttcgcttttg gcatccaaca attcgcccaa
    53461 ccgccgtttg gatgcttcgg taaattttcg ggaatccatc attttccttt tcaaatgggt
    53521 tttgcgccct attatagtgg attaaattta aatcaggaca aggcggcgag ccgcaggcag
    53581 tacaaatagt acggcaaggc gaggcaacgc cgtaccggtt taaatttaat ccactatatc
    53641 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
    53701 aatcgtaccg ctgccctgcg ccttcggttt ggcggcacta aattccagct tgctcaaagc
    53761 ggaaaggtaa gctttggcgg tcgccgccaa aatgtcggta tccgcgccct gaccgttggc
    53821 gacgcggttg ccgcgcgcca aacggacgct ggtttcgcct tggctttccg taccttgcgt
    53881 gacggcgttg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tctcaatcgc
    53941 tttgaaaatt gcatcaacag ggccggagcc ggttgcggaa gcgcgttttt cttcgccttt
    54001 gatgccgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttgaga
    54061 aatgaattta tagctttcgg cattcatatt gcccatttcg tccgacacca gcgcgtgcag
    54121 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcgtg caaatgccgc
    54181 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
    54241 gttgcggccg gacaatttgc ccaagctcaa tcggtttgcc gaccaaccga ccgattcggc
    54301 agacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
    54361 gtgcgaaaag gcattcgcgc cgacaatggc tttattgggc tgcacgggat agcccgtaat
    54421 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
    54481 gaacaagtcg tggcgcacct tcaacgccat cacgatttct tcaaccgaag cattccccgc
    54541 gcgttcgccc aaaccgttaa cggtacattc cacctgacgc gcgccgcctt ttaatgcggc
    54601 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc agacgacttt
    54661 gccgccgttg ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tgtacgggat
    54721 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc
    54781 gcagatttcg gcaaggaaat cgatttgcga acgcaacgcg tcttcgcagg aaaattccac
    54841 atcgtcggtg tattcacgag cgatttttac cgctttgacc gccgcctcaa tcacctgctt
    54901 cggcttcatc ttcagcttgt actccatatg gatggggctg gtggcgatga aggtgtggat
    54961 gcgttttttc ggcgcgggcg caacggcctt acccgcctga cggatgtccc gctcgatggc
    55021 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
    55081 gaaatcgccc gggctggcgg cggcaaaacc cgcttcgatg atgtccgcgc ccaatttttc
    55141 caactggcgg gcaacgcgga ttttttcctc tttggtcata gcggcgccgg gcgattgttc
    55201 gccgtcgcgc agggtggtgt cgaaaataat aacgcggttg gcctgtgtca tttctgttct
    55261 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggtcttc cttgcgattc
    55321 ggcaagcgcg gtcaattttt gcagttgcgc caaaggcagc gagccgcgtg tgcgccattt
    55381 gtagatggcg gcggtcgtcg cggcattttc aggatcgtgc cgtttcaacg cttcggcaag
    55441 cgcgttcacg ccgccgaaat aggcgaccag acggtctata tctaattgca tggtagtcct
    55501 tgtctaaaat aaatcagccc gcagcagaaa tatggacagg attatacgca ttttggacaa
    55561 aacggcaata ttaatttgaa aaaatctcaa taagttgcta taaattttat taattttata
    55621 cattttgtct attttattgc agatgcggcg tgattgttga caattgcgac agaacagggc
    55681 aaattcggcg gtacaaggcg gcaacgagac aggacaaacc aacaaggacg gaaacaatga
    55741 aatacaaaag aatcgtattt aaagtcggca catcttcgat tacccattcg gacggcagcc
    55801 tctcgcgcgg caaaatccaa accatcaccc gccagcttgc cgcattgcat catgcgggac
    55861 acgagctggt cttggtgtct tccggcgcgg ttgctgcagg gttcggcgcg ctgggtttca
    55921 aaaaacgtcc ggtcaaaatc gccgacaaac aggcttccgc cgccgtcggg caggggctgc
    55981 tgatggaaga atatacggca aacctgtctt cagacggcat cgtgtccgca caaatcctgc
    56041 tcagccgtgc cgactttgcc gacaaacgcc gctaccaaaa tgccggcggc gcactttccg
    56101 tgctgctgca acgccgcgcg attcccatca tcaatgaaaa cgacacggtt tcggttgagg
    56161 agttgaaaat cggcgacaac gacacattga gtgcgcaagt ggcggcgatg atacaggcag
    56221 acctcttggt gctgctgacc gacatagacg gtctttacac cggcaacccg aacagcaatc
    56281 ccgatgccgt acggctggac aaaatcgaac acatcaacca tgaaatcatc gaaatggcgg
    56341 gcggctcggg ttcggcaaac ggcacgggcg gtatgctgac caaaatcaaa gcggcaacca
    56401 tcgccgccga atccggcgta ccggtgtata tctgttcctc actcaaaccc gattcattgg
    56461 ccgaagccgc cgaacatcag gcggacggct cgtttttcgt cccccgtgcc aaaggtttgc
    56521 ggacacagaa gcaatggctg gcgttctatt ccgaaagccg gggcagcgtt tatgtggacg
    56581 aaggtgcgga acacgctttg tccgaacaag ggaaaagcct gctgatgtcg ggcattgccg
    56641 gaatcgaagg gcatttttcc cgtatggaca ccgtaaccgt gtacagcaag gcaaccaaac
    56701 agcccctggg caaagggcgc gtccttttcg gctccgccgc cgccgaagac ctgctcaaat
    56761 cgcgtaaggc gaaaggcgtg ttcatccatc gggacgattg gatttccatc acacccgaaa
    56821 tacgcctgct tctgaccgaa ttttagaaaa ggaaacccat gtcaaacaca caaaaacagc
    56881 ttgcccttgc caaagcggca aaaaaatccg tcaacacggc ggatacggaa gaaaaaaacc
    56941 gcgcgctgct tgctatggcg gacagtctgg aagtggcggc ggaagatatt ttggcggcaa
    57001 accgtttgga tttgaaagcg gcggcaggca aaattcccga cagcatgatc gaccgccttt
    57061 tgttggacgg caaacgcatt tgcgcgatgg cggacggcat cagggcggtt gccgcgctgc
    57121 ccgaccccgt gggcgaaata ctggaaacct cgactctgcc caacggcttg gaaatcgtca
    57181 aaaagcgcgt ggcgatgggc gttatcggca ttatttacga aagccgcccg aacgttactt
    57241 ccgatgcggc ggctttggca ctgaaaagcg gcagcgcggt cgtactccgc agcggcaaag
    57301 atgcatttca atccgcacgc gccatcgttg ccgccctgaa aacggggttg gcacaaaccc
    57361 gcatcgaccc cgaagccgta cagctcatcg aagacacagg gcgtgagggc agttacgaaa
    57421 tgatgagggc gaaagattat ctagacctgc tgattccgcg cggcggggca gggctgatac
    57481 gggcggtggt tgaaaatgcc gtcgtgccgg tcattgaaac ggggacgggc atcgtccaca
    57541 tttatatcga caaggacgcg gattgggaca aggcactccg tatcgtgtac aacgccaaaa
    57601 ccggccgccc gtccgtgtgc aactcgatgg aagtgctgct ggtgcatgaa ggcattgctg
    57661 ccgacttcct gcccaagctc gaacggctgt tggttcgcgg ccgtatagaa gccggactgc
    57721 cgcccgtccg cttccgtttg gatccgcagg cggcgcggta tatcggcggc gaagcggcgg
    57781 gtgcagacga tttcgatacc gagtttttag actacatcct cgccgtgaaa accgtcgctt
    57841 cggttgaaga ggcggtcggg cacatcgaag cccgcggcac gcaccattca gacggcatcg
    57901 ttaccgaaaa ccgccacgct gcggactatt tcacgaccca tatcgattct gccgccgtgt
    57961 atatcaacgc gtccacgcgc ttcaccgacg gcggcgaatt cggcttgggt tgcgaaatgg
    58021 gcatctccac gcaaaaactc cacgcccggg gcccgatggg tttgaaagag ctgacgagtt
    58081 acaaatacat cgtacaaggc acgggacagg ttagggaata atccgaattc aaacgtctga
    58141 tatagtggat taaatttaaa ccggtacggc gttgcctcgc cttgccgtac tatctgtact
    58201 gtctgcggct tcgccgcctt gtcctgattt ttgttaatcc actataaaaa tgccgtctga
    58261 aacagtcttc agacggcatt tttatttgca aaaaaatcaa gcattgtcca aggggacgag
    58321 aatcgtacgg ttgccgttgt gttccggtgc ggacacaatg ccttccgctt ccatttggtc
    58381 aatcagacgc gcggcgcggt tgtagccgat acgcaaggcg cgctgtacgc ccgaaatgct
    58441 ggctttgcgc gttttcagga caacggatac ggcctcgtcg tacatcggat cggtttcgcc
    58501 gtcgccgctg cgcccgatgc cgggcagctc ttcgctgccg ccgccgctca aaatatcgtc
    58561 aacatagtcc ggctcgccaa actgtttcaa atattcgacc acgcggtgca cctcttcatc
    58621 cgaggcaaac gcgccgtgaa cgcgttgcgg ataggcagta cccggcggca ggaacagcat
    58681 atcgccctga ccgagcaggt tttccgcgcc catttggtcg agaatcgtgc ggctgtcgat
    58741 tttgctggac acttggaacg cgatacgcgt cgggatgttc gccttaatca gacccgtgat
    58801 gacatcgacg ctggggcgtt gtgtggcaag gataaggtgg atgcctgccg cgcgggcttt
    58861 ttgggcgagg cgcgcaatca gttcttcgat tttcttgcct gccgtcatca tcaaatcggc
    58921 aaactcatcg accacgacca cgataaacgg cagtttttcc aaaggttcgg gatcgtcggg
    58981 cgtgaggctg aacggattgc cgattttttc tccccttgct gcggcttcgg cgattttttg
    59041 gttgaagccc gcaagattgc gcacgcccat aaagctcatc agccggtagc gtttttccat
    59101 ttcgttaaca caccagttca gcgcgtttgc cgccagcttc atatcggtaa cgacaggggc
    59161 gagcaggtgc gtgatgcctt cgtaaatgct caattccagc attttcggat cgatcataat
    59221 catacgcacg tcttccggca cggctttgaa aagcatagac agaatcatcg cgttgacacc
    59281 caccgatttg cccgaaccgg tcgtgccggc aaccagcaaa tgcggtgctt tgcccaagtc
    59341 ggttacgacg ggctgtccgg tgatgtcctg accgagcgcg agcgtcagct tggatttgga
    59401 ttcggcaaac tcgggcgaat tgaaaatttc gctcaggcgt atcatttggc gtttcgggtt
    59461 cggaagttcc aaacccatgc aggttttgcc ggggatggtt tcgacaacgc ggatggaagc
    59521 cacgccgagc gaacgcgcca agtctttttc caaattcaga acggaattgc cgcgcacgcc
    59581 gacatcgggt tcgatttcat aacgcgtaat cacggggccg gaataagaat cgacaacctt
    59641 gaccttgact ttgaactccg ccaatttttc ttcgatggtg atgctgtttt ccaacagttc
    59701 ttcttcggtt tgcgtcgcct cgggattgaa cagcggaggc agaagcaggt cggttgtcgg
    59761 caggtgttcg gataccgcac cggtctcttc cgattggaac gccccttcat ccgcttccgt
    59821 atccgatacc cggcaggacg ggcgttcaga cggcacatct tcaaacggac aaaccgcctg
    59881 actgtcatgc ccgaacgctt cggtttccga cagatattgc ccgcttgacc gctctgccgc
    59941 accctcactg ccgtcatctg caatagcggc ggtttcctcc tgccaacctc cattcaaaac
    60001 atcagcggca agatggtcgg tttcggcaat gcggctgcgt tgcgcctgct cgaatcctgc
    60061 cggcggctca taggtacggt tgtagatttc cgatacggga ggcggcggca gaatatcgat
    60121 ttcgggtacg gctacctccg gcacttcggg tggttcgacc acccaagcat cgggcgcggc
    60181 aggctcttca atatggatat cggcagcttc atcataaacc ggaccgccgt atcccacaga
    60241 cgaaacggtt tccgtgaaaa cattttcggt ttcagacggc atttcgacat cccgtttccc
    60301 gacaaccgtc cgactttccg gaatcaggcg cgaaatccgc gcctccgcag aaaccgttgc
    60361 cttgttcggt tctttcaaat tgacggaaac gcggcggcgg gcatcgcgga cggctgtgcc
    60421 gtctgaaacg gactgccctt gggaaacatc cggggatttt gcctccgcct gccctttctc
    60481 ctgccctgca tgacggcgga acggcgcatt ccgtgcattt tcggctttga ctgcagaaaa
    60541 ggcagacgga tgctgcttct caaaatacgg ctcaaatccg taatccgcag actcggaaaa
    60601 cgcttctttg tccgcatcaa acatacggga atacgtgcgt tgaagcacag ggtcgtccaa
    60661 accgataatc tgcagccctt ccatcggggt atcggacacg cgtactttgg ggacggctgt
    60721 tccgtttttc tcaaatgcat cgatatagcg ttttttcgtt tcccttaaag ccgcgctgct
    60781 taaagcacgc gttgcttctt ccaaagtgat ttccttaaaa accggacgga cgggcgaagt
    60841 tttgctttca gactgcatca acccttcagc aatactccgg tcgaatggga tgcggcggtt
    60901 gtcgattacg gcagttgcaa tgtcttccgt atcggcagcc tcttcctccg cagcttctgc
    60961 tgcttccgtt tccgcttctt ccgtcccgtt tccactgtct gaatacccgt cggatgcggt
    61021 cggaacatcc gcacggtttg cagaagcagg cggaacgggc ctgtccggtt cggattcgtg
    61081 cgccagatag tttcggcaga aacggacgac accgacaaac agccaataca ctgccgtttt
    61141 gacggcatgg aaaagcatca gggaaaactc gggaaaatcg ggcataccgt ctttgatttc
    61201 aggcagctcc gcctgttttt cccctttctt ttcctgccac gcagaaacct cgcgcatcca
    61261 ctcgcgttcg gattgtgcgc ggacaaaaaa caggccggca agcgcaagca acaaaataac
    61321 gatcaaaact atccaaaaca tatgccgttt cccgcaagac aaacgattaa ccgagtttat
    61381 tttaacgtgt ttccagccca aaaaaaacaa ccggccggca attctgccga aaccgattcg
    61441 gctttcggtt tgcccccgcc gacagtataa tagccgcccc ttcccttaat ctttgcggca
    61501 ttaccatgat tttccaaaca gtctggtttt cagatgtggt actgtcggtt tcgtggattg
    61561 tccttatcct gatactggca gcttccgcgc cgtcggcatt ccgttcgctt gcccgctacc
    61621 gttccgccct tcccctgtgt accgtaattt tttccgcagc atggtgtctg aacgcctctg
    61681 tcggcggcgg acagcttgcc cagatgaact accacctgct tgccgtcaat ctggttacac
    61741 tgatggtgga tacctccgcc gccctttggc ttgccgcgct gctgatgctg ccctactgcc
    61801 tgctgtttgc cggttctgcc ggcgcatatc cgccgaacgc tttggtgctg atactgcccg
    61861 cgcttgtcgt caaccgcctg tcgcgtatgc tggtcaaccg tctgccgccc aatattttca
    61921 tctttatttt cgtcaacggc tttctcgctt ccgccgccgg cattctgctg accgggctgg
    61981 tgctgatcgg tattttggat gccgcaaacg cttttccgtc cgaaatattg tggacggccg
    62041 ccctgcccgt ctttattctg ctggcgtggg cggaagcctt cctcagcggc atttcaactg
    62101 ccattttcgt ggcactgaag ccccattgga tcaacacttt cgacgacaac cgctatctga
    62161 agtccgagcg cggcatatgg ctgtagcctt cagacggcat aggcagacaa aaccatgctt
    62221 tccaacatcc tccccctttc catcggtgca atattcggca caacggcgcg ttggctgctc
    62281 aacctcgccg tccccgcatc gttgtctccc gccacaggca acctgtttgc caactggacg
    62341 ggcgcgctgc tcatcggcat ctttgccgaa accgtcagcc atccgcaatg gaagctgctg
    62401 ctgattaccg gttttttcgg cagcctgacc acgctttccg gattttcact ggaaaccgta
    62461 accctgctcc aatcgaaccg tcccgcttcg gcacttgcca atattttcct acacacggca
    62521 ggttcgctgc tgctgacttg gctcgggctg aagataggga ctgcagtcaa ataaccgcaa
    62581 aaaattgctt cctttggttt tgacataatc tataaattgg cttcgcttgt ttaaatactg
    62641 cctgtgccaa ttatagtgga ttaaatttaa atcaggacaa ggcggcgagc cgcagacagt
    62701 acagatagta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc cactataccg
    62761 ttctcaaatt caaattgggc ttttaggtct ataacaaaga aaaagccttg ggaggataaa
    62821 cctaccaagg ctttttctta aaaaacgctc tatcactgtg cctaaaaatg caaaaaagta
    62881 accgtcataa tccttttgcc aaaatcaata tcatcatccg atgaatgaac ggacggtata
    62941 taagaaagcg ttaataaact cataaaccag taagaaaaat cagtaacgaa gatattcatt
    63001 attacccatt cttaaaaatt atcacgttcg tttgggtaat tatcataccc ttttccctct
    63061 tattactaaa gcacgaatta ctttaaagta cgcacccaga gacaagccga aaaggcatat
    63121 ccgatccgcc aaacaaaatg cccgccgact tactccggcg ggcggttcca acatcctgtc
    63181 aatccttcat cagccagtat tgcaaaccga ttaatgtttt accgtcttta atttcatcgt
    63241 ttgccaatgc ctgacggact tcatctttcg acatcagcac ggtttccgtt atttcatctt
    63301 catcattaga aagcgtactg cccaagcgca cgccttccgc ctcgaacaga tacatttttt
    63361 cattgcaaaa accgaccgcc gtataaaaac tgtacagcag gcgcacgctg tcggcaacat
    63421 aaggcgtttc ctccgccaat tctcgcagcg cacacgctgc catatcctcg cccgccacat
    63481 ccagcttgcc cgcaggaagt tccaatgtcg cctgatttgc cgcataacgc cactgccgca
    63541 ccaaaacgac cttttcgtca tccgttaccg ccaacacgca cgccgcgccc ggatgccgga
    63601 tgacgatgcg ctgcccttca ttgccgttgg gcagcctgac cttatccctg ctgatactga
    63661 cgaaaccgcc ctcataaatg gtttcgccgc ctaattttac ttccctcaaa tccacttcat
    63721 atctcctttc cgtttcctat tctgccgttc aatcttcacg cttgccgcgc tcgatttcca
    63781 ctccaacctc gcgcacgcct tccaaaatac ccggcttgac gattttcacg tgcacccaca
    63841 ccgcgccgaa atatcccaaa accaaatcgg caatatattc cgccaacgct tccaaaagca
    63901 ggaaatcctg ttctttcaga tgtcggcgca gcgtttcgca tacctcggca taatgcaccg
    63961 tattggcaat atcgtcgtcc gaacccgctt tctcgggaac gccgatgtcc aaatcgacaa
    64021 tcagggtctg caaccgttcg cgttcccagc cgtacacgcc gataagcgta tccgccttca
    64081 tgccgtgcaa aaagatttta tccataatcc gccccgtttt ttgctaaaat aaccgcacca
    64141 ttctaagtaa agtaccgcaa atgttcaaca taccggccgt cgccgtttcc tatttaatcg
    64201 gttcactttc tttcgccgtc atcgtttcca agtattacgg gatggacgac ccgcgcacct
    64261 acggatcggg caatcccggc gcgaccaatg ttttacgcag cggcaaaaaa aaggcggccg
    64321 cgctgacgct cttgggcgat gccgccaaag gtttggttgc cgttttgctt gcacgcgtgc
    64381 ttcaagaacc gctcggttta tccgacagcg caatcgctgc cgtcgcactc gccgcgctgg
    64441 tcgggcatat gtggccggtg tttttcggat ttaagggcgg caaaggcgtg gcaacggcat
    64501 tgggcgtgct tctggcactc tctcctgcaa ctgccttggt ctgcgcgttg atttggcttg
    64561 tgatggcatt cggcttcaaa gtatcctccc ttgccgcgct ggtcgccaca accgccgccc
    64621 cccttgccgc actgtttttt atgccccacc cttcttggat tttcgcaacc ctcgcaatcg
    64681 ccatattggt gttgctccgc cataagagca acatcctcaa cctaattaaa ggcaaagaag
    64741 gcaaaatcgg cgaaaaacgc tgattcggca tttgttttcc attacgtcat gccgtctgaa
    64801 accatgttcc cgcgttcaga cggcatttcc gcacaaacgg ctttcataca taccatgctc
    64861 agttacagac acgcattcca cgccggcaac cacgccgata tgctcaaaca cttcaccttg
    64921 tttttagtgc tgcaatattt caaccgcaaa gacaagccct actggtacat cgacacgcac
    64981 ggcggcgcgg gtttgtacaa tctcgaaggc agcgaggcgc agaaagtcgg cgaataccgg
    65041 caaggcatcg ccctgctccg acaggcgcaa aacctgcctg ccgaactctc tgactttgcc
    65101 gcgcacatac aaaaaatcct accctcgccc gaactttact gcggctctcc gtggctggcg
    65161 caatcgctga tccgcatcgg cgacaaattg cgcctgtttg agctgcaccc tgccgacttt
    65221 gtccatctgc aaaacaatat gggcgaagcg ggattgggca aacgcggaca agtgttgcgc
    65281 gaagacggtt acaaagggct gatttccctg ctgccgcccc cgccgcgccg cgccgccgtc
    65341 ctcatcgacc cgccctatga ggaaaaacag gactaccggc gtgtaacgga aacgctgaaa
    65401 gccgctttaa aacggtttga gtccggctgt tatctcattt ggtatccctg cctcagccgc
    65461 gaagaaagcc acaagctgcc ggaagaattg aaaaaactcc tgcccgacaa ctacctttac
    65521 gccgaactgc acgtccacgc gccgaaaacc gacggtttcg gtatgcacgg cagcggaatg
    65581 ttcgtgatta atccccctta ccttttagcc gaacaattgg cggcaaacct ccccgccctg
    65641 acgcggctgc ttgcgcaaga cgaaggcgcg cgctaccttt tggacagcaa aatccgctga
    65701 ccccgatgcc gtctgaaccc ttcagacggc atcttccgtc aaacaaacaa aaccgggcga
    65761 tacacactcc atatcgtccg gttctgcata aaaccagtat tcaaaaatca gaacacgctt
    65821 ttcattacac ccacggtacc aaccacaccg ttcagcagca tcagaccacc ggcagccgta
    65881 cccatacctt gacggattgc gtcatgctta ttggcacgtt tttcacgttc caaagccaac
    65941 tcctcagaaa catttttacg ttgacggcgg tgttgcgcca gttccgcacg gctaattcgc
    66001 gcttcggcac ggttacgacc agccttgatt acggccgaat cgtcgccgat agcatagttg
    66061 gctacagaat tttcagaagc gcaggcagca agcaaaacgg cacacacagc aacaacggaa
    66121 atatttttca agtttttcat acctgtccta ttatctcaaa ataaagaaaa aactaatcca
    66181 actggagttt tatcggataa aataaatatt gggtgaattt tcgtttgcct ttttgatggc
    66241 gcgtgccgca tccatcattt cttcccggcg ttctttacgg tattcggcac gctcggcacg
    66301 ctcgcttatc tgaatggcac gacttttcgc ctgtatttcc gctgcagatt cttgagttac
    66361 aaaacggcgg tctgcgtaag tacatgccgt caccaacatc gcagccaccg acaacaaaat
    66421 cagtttcttc attttcatct acctatattt aagtttattt gtaaaatatg ttatcactat
    66481 atatatatat aatgtcaata ttttttaaaa ataaatttgg catcttgtac ttctcgatcc
    66541 gcctgccgcg ttccgacttg ccccgcgccg tttttttcca ctacaatccc acgcgatatc
    66601 cggcggaacg caccaaagga acgccccgca gcaatctgcc gcctttcaga cggcacaaac
    66661 ctttccgaaa cgagcaaacc atggacacac tgacccgatt cctccccgaa cacctgcaac
    66721 aaaaccaact gcccgaagca ctcggcggcg ttttgctgtc cgtcgtttcc gcctgtaccg
    66781 aaatcaacgc caaagtccgc ctcggcgcac ttgccggcgt attgggtatg gcgggcacgg
    66841 gcaatattca gggtgaagac cagaaaaaac tggatgttat cgccaacaac attatgattg
    66901 acacactcaa agccaaccct gccgttgccg gtttggcaag cgaggaagaa gacactttcg
    66961 taagtgccgg tgaaaacgga cgctatctcg tcctattcga ccctttagac ggatcgtcca
    67021 atattgatgt caacatttcc gtcggtacga ttttctccat tctcgccaaa cccgaaggcg
    67081 cattggcaac cgaatcattc cttcaaacgg gcagacagca gcttgccgca ggctatgtcc
    67141 tctacgggcc tcaaacccag ctcgcattca cattcggaca cggcgtgtac gtatttacac
    67201 tcaatgccga aaacgaattt gtgctgacca aagaaaaccc gaaagtaccc gaaagtacca
    67261 aagaattcgc catcaatatg tctaaccgcc gccactggct accccccgtt caacaatacg
    67321 tcgacgagct cttggcgggc gaaaccggta cgcgcggcaa aaactacaat atgcgctggg
    67381 tagccagtat ggttgccgaa atccaccgca tcctgatgcg cggcggcgtg ttcatgtatc
    67441 tgcaagacaa acgcgacccg tccaaacccg gcaaactgcg cctgatgtac gaagccaacc
    67501 ctatggccct gatactcgaa caggcgggcg catcggcaag caacgcatat caagccatgc
    67561 tcgatataca gcccgaaagc ctgcaccaac gcgtcgccgt cattatgggc agcagcgaag
    67621 aagtggatta cctcaaccgt ctgcattcaa aataggcaga gaccgtactg ataaagaccg
    67681 gcggcagcgt gaatccgata ctgcgccgat atttcaagca taccctccgg aaaaacagaa
    67741 aactgccttt ccgaacgata aaaataccgt ctgaaaaatt ttcagacggt attttaaaaa
    67801 ttaaagctca aatttatttg cccgccgctt tttgcaggat ggcgtacaac tcgtctttca
    67861 gtttcaattt ggcttttttc agctcatcga tggtttccgc accgctggta accggattgt
    67921 tggccagacc ggtaatttta tcgtccagct cgttatgttc gtcgaacaga cgggcgaagc
    67981 gggaattttc ctgtttcaat ttggaaatca aatcacggta ttctgggaac atatcgcttt
    68041 cctctcttgg tttacggtta ataaagtcaa attgaatctt tacattaatt ataacaaaga
    68101 taatattttt atccagtgtt ttgttacaca cggcaacacc gtccgcagaa aaccgcccca
    68161 ccgtccgata aaatcgttga caatcttccg ctttacgcat aaattttcag cccttcctac
    68221 tgtatatgaa aggcacgccc cagtttcccc aatgtggatt ttcagcgcgc gcagtagagg
    68281 tgccgaccca aattgacaga ccctttgctt ttgttaatat cctagaaaat ccagaaattc
    68341 gcagtactct accactcatc gccaattggc caacctttcc acggctttgg gttaatggtg
    68401 agttaatcgg tggtagtgat attatcctac aaatgtacca atcaggtgag ctaaaaccac
    68461 tcattgaaga gcatagccca aaggtctgat tacttaatat cttaaaataa atatcaaaaa
    68521 agactgctga tgacattagc ggtctttttt tgatggtcaa gattgatagt ttggattgaa
    68581 ttatttagcc atattacata caataatcag catgctttgt atgaattatc aatcaaactc
    68641 aaactcaggc gaaggagtgc ttgtggctaa aacatattta ttgactgcat tgataatgtc
    68701 tatggtaatc tccggatgtc aagtcatcca tgccaatcaa ggtaaggtta atactaattc
    68761 tgctgtcatc gcaggtgcag acgctcacac gcctgaacat gtaacgggac tgaccgaaca
    68821 aaagcaggtg attgcaagtg attttatagt agcgtcagcc aatccattag caacacaagc
    68881 tggctatgat atcttaaagc aaggcggtag cgctgcagat gcgatggtgg cggtgcagac
    68941 gacactaagc ttggtagagc cacagtcgtc aggcttgggc ggtggtgcat ttgtgttgta
    69001 ttgggacaat accgccaaaa cattgaccac atttgatggg cgtgagacgg caccgatgcg
    69061 tgcgacgcca gaattatttt tggataaaga tggttaacca ttgaaattta tggaagcggt
    69121 ggtcgctcgg taggtacgcc tgctatccct aaactgacgg agacaataca tcagcgatac
    69181 ggtgtattgc cttggggaaa attatttgat acgccgatcc acttggcaaa acaaggcttt
    69241 gaggtgtcgc caaggcttgc catctcggtt gagcaaaatc agcagcattt ggcacgctat
    69301 ccaaaaacag ccgcttattt tttgccgaat ggtgtgccgc tacaagcagg cagcttgctg
    69361 aaaaatttag aatttgctga cagtgttcag gcgttagcag ctcaaggtgc aaaagctctg
    69421 catactggta aatatgccca aaatattgtt tcagttgtcc aaaatgctaa ggataacccc
    69481 ggtcaattat ccttgcaaaa tttatctgat tatcaagtgg tggaacgccc gcctgtttgt
    69541 gtgacttatc gtatttatga agtatgcggt atgggtgcac caagctcagg tgggattgct
    69601 gtgggtcaga ttttggggat tttaaatgaa ttttcaccca atcgggtggg gtatgatgct
    69661 gaaggtttgc gtctcttggg cgatgcttct aggcttgcgt ttgcggatcg tgatgtatat
    69721 ttgggcgacc ctgattttgt accagtaccc attcgccagt tgatttctaa agactaccta
    69781 aaacatcgca gccagctgct tgagcagtcg gataaggcat tgcctagcgt atctgcaggc
    69841 gattttattc atgagtgggc gtcctcacaa gcgattgagt taccttctac cagtcatata
    69901 tcaattgtgg ataaggcggg caatgtgcta tcaatgacga cttccattga aaatgcgttt
    69961 ggctcaactt tgatggcaaa tggctacctg ctgaataatg agctgacaga ctttagtttt
    70021 gagccaataa agcagggcaa acaggtcgcc aatcgtgtcg agcctggcaa gagaccacgc
    70081 tcctcaatgg caccaaccat cgtatttaaa gcaggcaagc cttatatggc aatcggctct
    70141 ccaggtggta gccgtatcat tggctatgtc gctaagacga ttgtggcaca tagtgactgg
    70201 aatatggata tccaagatgc catcagtgca cccaatttat taaatcgctt tggtagctat
    70261 gaactagaaa cgggtacaac cgccgtccag tggcaacaaa cattaaatga tttgggctat
    70321 aagactgatg tgcgtgagct aaattcaggt gttcaagcca ttattatcga gccaagcagg
    70381 ctggttggtg gtgtcgatcc acgccgagaa ggtcgagtga cgggtgatta gaggtgaagc
    70441 ttgtattttg tgaatttgac cgcattaagt taattattaa aataggggga aattgtgagt
    70501 aatcaaacaa aaatacaagc catcaagcaa gcttctgagc agatcttggc aatttgtgag
    70561 acgccaaaca cagcattaca agccattcat ctgattttaa gacatggcgg ggcaggaaga
    70621 attgtcttgg caagtggtgt atcagcgggt tatggctgat aaagatgtgg ttggtgctgg
    70681 ttatttgatt gattttgcac aaactgccga aaatttacca tttgatgtat tgcctttgat
    70741 tagccttgtg ctaaataaag gtgatgagac gctaaaaacc ggcatgttaa ataaattacc
    70801 tgataatgcc aaagaaaatt tacgcattat gggctatctg ccttaggttt tgaagtattt
    70861 ttttatacga aaaacgctca tgataccctg attacacaaa ggagccgaga atatgttttc
    70921 aaaaagcgtt accctcgcac aatatgaccc cgatttggca gcagccattg cccaagaaga
    70981 ccgacgccag caagaccacg tcgagctgat tgcctctgaa aactacgtca gctgcgccgt
    71041 gatggaggca caaggttcgc aattgaccaa caaatacgcc gaaggctatc ccgcaaaacg
    71101 ctactacggc ggctgcgaat acgtcgatat tgtcgaacaa ttggcaatcg accgcgtaaa
    71161 agaactgttt ggcgcagcct atgccaacgt tcaaccgcac tccggttccc aagccaacca
    71221 agccgtatat gcttccgttt taaaaccggg cgacaccatt ttgggtatgt ctctggcgca
    71281 cggcggacac ctgacccacg gcgcgagcgt taatatttcc ggcaaactct acaatgccgt
    71341 tacttatggc ttggatgaaa acgaagtcct cgattatgcc gaagtcgaac gcctcgcgct
    71401 cgaacacaaa cccaaaatga ttgtggcggg cgcgtctgcc tacgcgttgc aaatcgactg
    71461 ggcaaaattc cgcgaaatcg ccgataaagt cggcgcatac ctctttgtcg acatggcgca
    71521 ctatgccgga ctggttgccg gcggcgaata tcctaacccc gtgccattct gcgacttcgt
    71581 aaccactacc acccacaaaa ccctacgcgg ccctcgcggc ggtgtgattt tgtgccgtga
    71641 caatacccac gaaaaagcgc tgaactcttc catcttccca agcctgcaag gcggtccgtt
    71701 gatgcacgtt atcgccgcca aagccgtagc gtttaaagaa gcgttgcaac ccgagttcaa
    71761 acaatacgca aaacaagtga aaatcaatgc tgccgtcatg gcggaagagt tggttaaacg
    71821 cggtttgcgc atcgtttccg gccgcaccga aagccacgtt ttcctcgtcg acctgcaacc
    71881 gatgaaaatc accggcaaag ccgccgaagc cgctttgggc aaagcgcaca tcaccgtcaa
    71941 caaaaacgcc atcccgaacg atccggaaaa accgttcgtt acctccggca tccgcatcgg
    72001 ttccgccgcc atgaccactc gcggctttaa cgaaaccgac gctcgcgtat tgtcgaactt
    72061 ggttgccgat gtattggcta atcccgaaga cgaagccaac ctcgccaaag tctgcggaca
    72121 agtgactgcc ttgtgcgaca aataccccgt ttacggaaac taaacgcccg atgtttccat
    72181 acaaaatgcc gtctgaacat tttccagacg gcattttttg ttttcggtaa cattgctatt
    72241 ttttctcttc cagcaaaatg accaactttt tcaatactgc gatttcacta tctttctgcc
    72301 tgatcaattc atcctttgca tccagcagcg acctcaattt ttcattttca aacaccagca
    72361 tttcatcaga gccgataata ttatgatggt tattggtatt gtcgccgata gagaagaaaa
    72421 acgatcggtt ctgctccgcc agcagctcaa ccacatcgat attgaaaatc tgggcaatct
    72481 ggcgcagctt gtcgagattg atgccgcttt tcccgcgttc tattttcgaa tacccgttaa
    72541 ccgacatttc caatttttcc gccatctctt cctgcgtcca ctgattgact tcccttaacg
    72601 tccgaatctt gtcatgcact tccatcctgc cctccaaggt taattttcac cactcaaaca
    72661 accgtaaccg gtatcttcag ttgatggtcg gatattttaa aaataaataa tataacacaa
    72721 tagtattttg ataaaaattt acctaacgag gtacagacat gtattcagaa aacaagccta
    72781 aacgcaccgt aaaaatgaaa aaatactggc agcctgcacc ctttccctgt ccctttctgc
    72841 ctgtacttcg actcaagtaa cttcttttgt cgagggactc tccgagggac tcgtagatgt
    72901 cagcgaagcc tttatcacaa caaaggcaga atcatattct gccccgtctc ttcaggataa
    72961 gcaaattttc ataaatcgcg tctcagaacg agcacaaaaa aattacaaag aaagaaggaa
    73021 aaagaaagaa gcagacttac taaattcagc atcgttatta aatgaatcaa catcagaagc
    73081 cctatctgcc cttaaaaacg ctatcgtaat tcctaaacgc cctaaacgct ccttgggcaa
    73141 aaaatataaa aacggttttc atcctgtaaa tacgtgtgcg gatgtggaag ctcggtgggc
    73201 aaacggagca atgagagatg ttagggacat agaaaggcta ataagccgaa acgtcccttt
    73261 acgtcgtatc ggagacgcca ttcaaaacat gtgcatggaa accaatgtgt ccattaggat
    73321 gtatcgccag gagtgtcgtt cagaatcgtc agatttttat gacgaaatgc ttgagcatca
    73381 cgagaatttc ataggatcct ccaaatcatt cgcatcatca cttcttcccc ttagcgttac
    73441 aaactttaca acagacatgg cgtgtactcc cgaattttaa tctaatcaca ctccacaact
    73501 ccctatcaat ataactgcta cttctctgag tagtatcaac caacccttaa caaaggacta
    73561 aaaaatgaaa aaagcactgc ttgcactgac tattgccgcc atctccggta ctgctatggc
    73621 ccagttgccc gactttctgg gtaaaggcga atataccgtc cgtacagaca tctccaaaca
    73681 aacgctgaaa aatgccgatt tgaaagaaaa acacaaagta caaaaaaaca tcggtttccg
    73741 tgccgatatg ccgtttgacg atattcacca cggcatgcgt ttcgaagtat cacacagccg
    73801 agacaaaaaa gacatgtacg ttgtgaccga aagtactact aaaccattcg gcaaagacgt
    73861 taaagaaaaa cgcaccgacg tgtatgccgg ttacacctac acccaaccgc tcagtgaagc
    73921 caccaaactg cgtgccggtt taggtttggg ctatgaaaaa tacaaagatg cggtagctaa
    73981 tgagaaagga acagtcagca ccgagcgcga agccttctat accaaagcgc acgccgacct
    74041 gacttccgat ttgggcggcg gctggtatct gaatccttgg gcagaggtta aggttgacct
    74101 ggacgccaaa ttgaaacaca acgcaactgt agccggtgtc tcagccgaca ttaacgcaaa
    74161 aacacgcgga tggggcgtgg gcgtaggtgc gaatatcggc aaacaaatta ccgataccgt
    74221 cggcatcgaa gccggcccgt tctacaaaca ccgtcacttc aaagcatccg gcagcttcgt
    74281 tttagacggc gggaacatcc gagtcgatcc taccaaaatc aacgaatacg gtgttcgtgt
    74341 cggcgtgaaa ttctaatcgc ggcgatatgc cccagacaca aaaccgcaag ccttttggtt
    74401 tgcggttttt gatgttgtag cagaccctaa taccgtctga aacttttcag acggcatttt
    74461 tccaatcggt taaaataccg cctcgacatt gtgttttgga aaacccgtgc ttgcttctgt
    74521 gattgatttt atcctgcata tcgaccaaca cctgctcagc ctgtcggcgc aatacggtgt
    74581 gtggatttat gcgattctgt ttttgattgt tttttgcgaa accggtctga ttgttacgcc
    74641 cttcctgccc ggcgattcgc tcttgtttgc cgccggcggg attgccgcgc tgggcggtat
    74701 ggatattcat ttgatggttg cattattaag ccttgccgcc atactcggcg acgcgctcaa
    74761 ttttaccgtc ggcaaatatt tcggcggcag gctgttcgcc aaccccgatt ctaaaatctt
    74821 ccggtgcgaa tacctcggca aaacccgccg tttttacgaa aaacacggcg gcaaaacgat
    74881 tatcatcgcg cgcttcgtgc ccatcgtccg caccttcgcc cccttcgtcg ccggtatggg
    74941 aaaaatgcac tatgccaaat ttatccgcta caacatcatc ggcggcttgt tgtgggttat
    75001 cttgttttct tacgcgggct atttctttgc caacttcccc gtcgtaaaaa acaatctggg
    75061 cttggtgatg ggcggcatca ttattgtttc cgtcctgccc ggcatcatcg aaatcgcccg
    75121 cgccaaactt gcggcaaaat ccgaacgtta attgttggaa attgacatta tgaatatatt
    75181 atccgtagaa aacgcttcct ttgccgtcgg acatgtcgcc ctgctcgaca aaacgtcctt
    75241 ccaactcgac agcggcgaaa aagtcggctt gatcggacgc aacggcgcag gcaagtcttc
    75301 gtttttaaaa atcctcaccg gcgtgcaaaa actcgacgac gggcagatta ttgttcaaaa
    75361 caatctcaaa atcgtttatg tgccgcagga gtcctttttt gataaggacg caaccgtatt
    75421 tgatactgtt gccgaaggtt tgggcgaaat tcgcgattta ttgcgccgtt atcatcatgt
    75481 cagccatgag ttggaaaatg gttcgagtga gctcttattg aaagagctta acgaattgca
    75541 acttgaaatc gaagcgaagg acggttggaa actggatgcg gcagtcaagc agactttggg
    75601 ggaactcggt ttgccggaaa acgaaaaaat cggcaacctt tccggcggtc agaaaaaacg
    75661 cgtcgcctta gcgcaggctt gggtacagaa gcccgatgta ttgttactgg acgaaccgac
    75721 caaccatttg gatatcgacg cgattatttg gttggaaaac ctgctcaaag cgtttgaagg
    75781 cagcttggtc gtgattaccc acgaccgccg ttttttggac aatatcgcca cgcgcattgt
    75841 cgaactcgat cgcggtattt tgcgctccta tcccggctcg ttctccaaat acagcgagaa
    75901 aaaagcgcaa gagttggcgg ttgaagccga acacaaccgc ctcttcgaca aattccacgc
    75961 gcaggaagag gcgtggatac gcaaaggcat cgaagcgcgc cgtacccgca acgaaggccg
    76021 cgtgcgccgt ttggaagaac tgcgccgcca gcgtgccgaa cgccgcaatg tacaaggaca
    76081 ggtcaacttc aaactcgaca gcggcaagaa aagcggcaaa atcatcgccg aactggaaca
    76141 cgcttcgttt gcctatgacg acaaagttat catggataaa ttttccgcca tcttgcagcg
    76201 cggcgacaaa atcggcttaa tcggcccaaa cggtatcggc aaaaccacct tccttaagct
    76261 gattttgggc gaattgcagc cgacctacgg cagaatccgt atcggcagca agcaggaagt
    76321 cgcctatttc gaccagttcc gcagcgcctt gaatgaaaac gatactgtgt tttacacgct
    76381 cggacagggc aacgattacg tcgaagtcgg cggcaagaaa aagcatgtga tgagctattt
    76441 ggaagatttc ctgttccccc ctgcccgcgc gcaaagtcct gtttcttcac tctccggcgg
    76501 cgaacgcaac cgccttttgc tggcaaaact ctttactcgt cccgccaata ttttggtttt
    76561 ggacgaaccg accaacgact tggacatcga cacccaagaa ttgcttgaag atctgttgcg
    76621 cgattaccaa ggcacggtct tcctcgtctc gcacgaccgt atgttcctcg acaatgtaat
    76681 tacccaaagc attgttttcg aaggacaagg ccgtctgaaa gaatacatcg gcggctatca
    76741 ggactatatc gacgcaaaat cgcgggaaga taaaattcag acggcatccg cacccaaagc
    76801 atccgatgtc gaacccgcca aagaaaaacc caaagccaac cgtacggtca aactttccta
    76861 caaagaacag cgcgaactcg acgccctgcc cgacgaaatc gccgccttgg aaaccgaaca
    76921 ggcagaaatc aacgcgcagc tttccgatcc cggaattttc aaagattacg aaaaagcagg
    76981 agcattgcaa aaccgcgccg aagaaatcga aatgctgctt ttggaaaagc tggaacgctg
    77041 ggagctgctg gaaaccaaac agaacggcaa tgccgtctga aatcaaacag gaagaaaaaa
    77101 aatgttcaaa cgacctgaag aaatcatcgt cctgatactt gccgtcctct ggattgccgg
    77161 tacgtatttt ctcgccgccc tgtttggagc ggatgcttat accgtgttaa aaatcactgc
    77221 ccttaccctg ctttggtctg ccgcatcatt cctcctttgg caaaaaaaac ctcagccggc
    77281 atatctggcc gctgctgctc ggctgcctga ctgcctgttg gtggccgtat ctgaatcaat
    77341 cggtcgcacc cagttcttta ccttggcatg catcatggac gttcaaaacc atcttagccc
    77401 tgattcccgc aaccgcaggt tatctgttta aatggaaaca ataccggcag cgcaaaacaa
    77461 agccgtaagc ccgtcttttt tcagacgcca tctccggtca gactgtcaac aattccgata
    77521 aaatgttata ctcgcgcccg ttgccgcgcc gtccgaaacc gccttttggg aaagcagaaa
    77581 tgatcagcaa acaagaatac caagcccaag ccgcccaagg ctacaaccgc atcccgctcg
    77641 tgcaagaact ccttgccgac ttggatacgc cgctttccct ctatctcaaa ctcgccaacc
    77701 gcccctatac ctacctgctc gaatccgttg tcggcggcga acgtttcggc cgctattcct
    77761 ttatcggcct gccttgcagc cactatctca aagccggcgg caaacacgtc gatgtttatc
    77821 aaaacggcga aatcgtcgaa caatacgacg gcaatccgct accctttatc gaagctttcc
    77881 acaaccgttt caaaacaccc gaaatcccaa gcctgccgcg ctttaccggc ggcttggtcg
    77941 gctacttcgg ttacgaaacc gtctacaatt tcgaacactt cgcccaccgc ctgaaaaaca
    78001 ccgccaaagc caacccgctc ggcacgcccg atattttgct gatgctgtcg caagagttgg
    78061 cggtaatcga caatttgagc ggcaaaatcc acctcatcgt ttatgctgat ccctcgcagc
    78121 ccgacagcta cgaacgcgcc cgcgaacgcc tcgaagacat ccgcacccag ttgcgccaaa
    78181 gctgcgccat cccactttcc ctcggcagca aacaaaccca agcagtcagc gagttcggcg
    78241 aagaaccttt caaagcctgc gtcgataaaa tcaaagacta cattttcgca ggcgactgta
    78301 tgcaggtcgt ccccagccaa cgcatgagca tggaatttac cgacaatccg cttgccctct
    78361 accgcgccct gcgcacgctc aacccttcgc cttacctctt ttactacgat ttcggcgatt
    78421 tccacatcgt cggttcctcg cccgaaatcc tcgtccgccg cgaacgcgac gacgtcatcg
    78481 tccgccccat cgccggcacg cgcctgcgcg gcaaaacccc cgccgaagac cttgccaacg
    78541 aacaagattt gttaagcgac gccaaagaaa tcgccgaaca cgttatgctt atcgatttgg
    78601 ggcgcaacga tgtcggacgc atcagcaaaa caggcgaagt caaagtcacc gacaaaatgg
    78661 tgattgaaaa atactcccat gtgatgcaca tcgtttccaa cgtcgaaggc tgtctgaaag
    78721 agggcgttac caatatggac atcctcgccg ccaccttccc tgccggcaca ctctccggcg
    78781 cgccaaaagt ccgcgcgatg gaaatcatcg aagaaatcga accggagaaa cgcggcatct
    78841 acggcggcgc cgtcggcgta tggggcttca acaacgatat ggatttggca atcgccatcc
    78901 gcaccgccgt gattaaaaac aataccctct tcatccaaag cggcgcgggc gtggttgccg
    78961 actccgaccc aacttccgaa tggcaggaaa ctcaaaacaa agcccgcgca gtggttcggg
    79021 cggcgcagat ggtgcaggaa gggttggata aataaatttc agacaacctt tgcagatgaa
    79081 aagaaggtcg tctgaaaatc acaaaacaac atcagagata cttacataaa aaatcacaat
    79141 gcaattcaca tcattagaaa tttgtgccgg agcaggcgga caggctttgg gcttagaaag
    79201 ggcaggcttt tcccatgttg cgttaatcga aatcgaaccg tcggcttgtc aaaccctacg
    79261 tttaaaccgt cctgattgga acgttattga aggagatgtc cgcttgtttc agggcgaagg
    79321 atatgacggt attgatttat tggccggcgg cgtgccttgc cctccttttt ccaaagctgg
    79381 aaaacaactg ggaaaagatg atgaacgcga tttattccct gaggctattc gtctagctaa
    79441 ggaaactgac cctaaagcca tcatgttaga aaatgtgcgg gggttacttg acccaaaatt
    79501 tgaaaattat cgcaaccata ttacagaaca atttgccaag ttgggttacc ttggacaatg
    79561 gaaactactt tatgctgccg attacggagt atcgcaactc agacccaggg ttttatttgt
    79621 tgcgttgaaa aacgaataca ccaatttttt caaatggcct gaaccaaatt cggaacaacc
    79681 gaaaaccgtt ggagaactgc tttttgactt aatgtcagaa aataactggc agggggcgca
    79741 taactggcgt ttgaaggccg cgcagattgc accgaccttg gtcggcggtt caaaaaaaca
    79801 cggcggtgct gacttggggc ctacacgatc caaacgcgca tgggcggagt tgggtgtgga
    79861 tggttcaggt ttgtgggata gtgcgccgcc tgaagacttt tcgggaatgc ctagattgac
    79921 tgtccgaatg acggcgcgta tccaaggatt tcctgataac tggcaatttt ttggtaaaaa
    79981 aacgcctatg taccgccaaa tcggcaacgc ttttcccccg ccggtagccg aagcggtggg
    80041 caggcagatt atcaaagcct taaaaaagga aaattgaatg aatccgcttt ttacccaaga
    80101 acgcaggatt ttccataaga aattattgga tggcaatatt cttgcaacca acaatagagg
    80161 cgtagtcagc aatgcggatg ggagcaatac ccgaagtttc aatatagcca aggggattgc
    80221 cgacttactg cattcggaaa cggtttcgga aagattaccc ggtcagacat cgggaaatgc
    80281 ttttgaagca atatgcagtg agtttgtcca atctgctttt gaaaaattgc agcatatcag
    80341 acccggcgac tggaatgtta agcaggtcgg ttctcgcaac cgtctggaaa tcgcacgtta
    80401 tcagcaatac gcacatctga cagccttagc caaggctgcc gaagaaaacc ccgaattggc
    80461 tgccgccttg ggcagtgatt acaccattac tcccgatatt atcgttacca gaaatttgat
    80521 tgcagatgca gaaatcaacc gtaatgaatt tttagttgat gaaaatattg ccacatatgc
    80581 cagtttgcgt gcgggtaatg gcaatatgcc gctgctccac gccagtatct cttgtaaatg
    80641 gacgattcgc agcgacaggg ctcaaaatgc ccgttctgaa ggattgaatt tggttagaaa
    80701 cagaaaaggc agactgccgc acattgttgt cgtaacggca gaaccaacgc caagccgcat
    80761 ttcatctatt gccttgggta caggggaaat agattgcgtt taccattttg ccttatatga
    80821 gctggaacaa attttacaat cattgaatta tgaagacgct ttggatttgt tttacatcat
    80881 ggtcaatggt aaacgattaa aagatatttc cgaccttccc ttagatttgg cagtttaaac
    80941 agaattattc cactatgaac acacccccca tccttcctcc cgccatgctc ggcatcctcg
    81001 gcggcggaca attaggcaga atgtttgccg ttgccgctaa aaccatgggc tacaaagtaa
    81061 ccgttctcga tcccgacccg aatgcgccgg cggcggaatt tgccgaccgc catttgtgcg
    81121 cgccgtttga cgaccgggcc gcgttggacg aattggcaaa atgcgcggcg gttacgaccg
    81181 aatttgaaaa cgtcaatgcc gacgcgatgc gctctctggc aaagcatacc aacgtttccc
    81241 ccagcggcga ctgcgtgtcc attgcacaaa accgcattca ggaaaaagcg tggatacgca
    81301 aagcaggctt gcaaaccgcg ccgtatcagg cggtttgcaa ggccgaagac attactgaag
    81361 caagcgcgca atttttgccc ggcatcctga aaacggctac gttgggctac gacggcaaag
    81421 gtcaaatccg cgtcaaaacg ttggacgaac tcaaagccgc gtttgccgaa cacggcggcg
    81481 tggattgcgt tttggaaaaa atggtggact tgcgcggcga gatttccgtg atcgtatgcc
    81541 gtctgaacga tgaaaacgtg caaaccttcg accccgccga aaacatccac gaaaacggca
    81601 tcttggctta ttccatcgtc cccgcgcggc tgagtgccga cgtgcagcaa caggcgcggc
    81661 agacggcgca acgcttggcg gacgaattgg attatgtcgg cgtattggcg gtagaaatgt
    81721 ttgttgtcgg cgacacacat gaattgctcg tcaatgaaac cgccccgcgc acgcacaatt
    81781 ccggccacca tacgatagat gcctgcgccg cagaccagtt ccaacagcag gtgcgcatta
    81841 tgtgcaacct gccgcccgcc gacaccaaat tattatcccc ttgctgtatg gcgaatattt
    81901 tgggcgacgt ttggcaggaa gatggcggcg aaccggattg gctgccgttg caaagccggc
    81961 cgaatgcaca cctgcaccta tacggaaaaa aaaccgcaca gaaaggtcgg aaaatgggac
    82021 actttaccgt tttgaccacc gattcggaca ccgcatttca agaagcaaaa aaactgcatc
    82081 agtccctata atccgaccaa cggcacagat gccgtctgaa gccgccgaca taccgccatg
    82141 agcctgctga cactgttacg ccccgccacc gtgcaagact gcaaagacat cttcaaagtc
    82201 cacctgcact ccgtccaata cacctgcatc ctcagctaca acgaacacgc cctaaaagta
    82261 tgggaaggct tgttaaacac agaaagctac ctgccgacca tttccgatcc cgacaaagca
    82321 ctttgggttg ccgaatacaa aggcaacata cagggatttt tccaaatcga ctgccaagaa
    82381 gcacaactcg atgcgcttta cgtccacccc ctgttccaca atctcggact gggcactgcc
    82441 ctgctccgtc aggcagaaac catcgcccac aaatccggtt tgagctttct gaaactctac
    82501 gcatctctca actccgtccc gttttacctt ctcaaccgct acgaatcgct cggttcggca
    82561 gtattgcagc ttgaccccag catcaaaatc aaatgcgaac tgatgcgcaa acacctctaa
    82621 tcccgcctta taaccaaaac tgataatctt caaatcaaac attatttcca caagcgcaga
    82681 cagccgtata aagttgcccc atctaccaac cgacaggaca agcatatgaa aacctacgca
    82741 caggcactct ataccgcagc cctgcttacc gcctgcagcc ccgcagccga ttcaaaccat
    82801 ccgtccggac aaaatgcccc ggccaatacc gaatccgacg gaaaaaacat taccctgctc
    82861 aatgcctcat acgatgtgac acggtatttt tacaaagaat acaaccactt atttatcaaa
    82921 acataccaat ccgaacaccc cggcacatcc gtcagcatcc aacaatccca cggcggcttc
    82981 agcaaacagg cattatccgt agccaacggc cttcaagccg atgccgtaac catgaaccaa
    83041 tcttccgaca tcgacctgct cgaaaaaaag gactggtaga aaaaggctgg caacaagccc
    83101 tccccgatca cgccgcaccc tacaccagca ctatggtttt ccttgtccga aaaaacaacc
    83161 ccaaacagat ccgcgattgg aacgaccttg ccaaagacgg cgctaacatc gtcatcgcca
    83221 agacctcggg caacggacgc tacgccttcc tcggcgcata cggttacggt ctgaaagcca
    83281 acaacggcaa cgagcaggaa gcccaaaaac tcgtcgcatc catcctcaaa aacacacccg
    83341 tttttgaaaa cggcggacgc gccgccgcca ccaccttcac acaacgcaac atcggcgacg
    83401 tactcatcac ttttgaaaac gaagccaact acgtcagcaa aaaactgacc caaggacagt
    83461 ttgaaatcgt ctatccgagc tacaccattt ccgccgaaag ccccgttgcc gtcgtcaaca
    83521 gcgtcgtcgc gaaaaaaggc acgcaaaaaa ccgcccgcgc ctatctcgaa tacctttgga
    83581 gcgaaccggc gcaagaactc gccgcctcac tctacctgcg tccccgcaac cccgaagtat
    83641 tggcacgaca caaagccgac ttccccgact tagacacctt ccctcccgaa gaaaaattcg
    83701 gcggatggga caacatcatg aaaacctact tcgccgacgg cggcgtattc gaccgcctga
    83761 ccgcacaaaa atgaccatta tgccgtctga accgcctttc agacggcatt tcacacatca
    83821 aaacagaaaa ccgcacccaa aatcatcatc acaagacaca acggcaaacc ggatacaaaa
    83881 cccaacaccg ccgccaaaat gccgtctgaa gccgtcggcg caaccatcct tatctggggc
    83941 gcgcctcatc aaaggggcgc ataactttcc ttaaccatcc cttttgacag ccaaataaac
    84001 agtgcttttg cctgtcagaa acaaaaccaa tattttgcgg ttcgtatcgg aacatttata
    84061 aaacatcaga tttccgtgtt tcgcgtttcc ggcaattcaa gattagcaaa accattaacc
    84121 tgcttcgcaa ggcaggtttt taaaacttta tcaggcgttc gagcatatcg gttcgaccga
    84181 cccgcatttc cggcaatcaa cactgccgac ggttgccgca tttctttttg atatacaatc
    84241 acactttcaa aaaacatacg acggcaacaa aatcatcctc cccccgcttg gcaaacatcc
    84301 ggcaaattaa aaccccgccc gaaacagcca tcggacaaat aacaggaaaa aatatgaata
    84361 ccgacttcca aaagatcctc aaacaactgg gcaaacaggc caaaaaagaa gcggcagaaa
    84421 aacaggaagc cgaaaaaaac aagcaaaaac aagaatatga tttggatttt tcacaagcag
    84481 tcggacaagt ctccccatta aaaaaccggc agcaatatta tgcgcagtcc gacaaaatcc
    84541 ccatcaaagc ccgtcccaaa gacaaccgca ccgacgaaga aaactacttc tacataggca
    84601 gcacatacaa cgacccaccc gccagcttct gcaaaaacgg acagggaaaa aacgacatcc
    84661 aacgcctgaa aaacggatac tatcccgtcg ttaccgatgt cgacctgcac ggctacaccc
    84721 aagaagaagc acaaaaagtc ctcaacgaat tcatcgcatt cacccaaaaa cgcggcgtat
    84781 gcggcgaaat catccacggc agcggattag gttccaaagg ctacaaaccc gttctgaaaa
    84841 acatgacccg aaactggctg atgcaacacc ccgacgtact cgcctacgtc gaaccccggg
    84901 aaggcaacga cggctgcgtc cgcatcctgc tcaaacgcaa attaaggcag caagactgac
    84961 aaatttctcc gacagacttg gcagcaccgc acaatatccg tataatttgc aaaccatttc
    85021 tgggggcgac cttggtttcg acgggggttg cgaagcagat gcgggcatac cggggtctca
    85081 gattcccgta aaacactgaa ttcaaatagt cgcaaacgac gaaacttacg ctttagccgc
    85141 ttaaggctag ccgttgcagc agtcggtcaa tgggctgtgt ggcgaaagcc accgcaacgt
    85201 catcttgcat tgactggttt ccagccgggt tacttggcag gaaataagac ttaaggtaac
    85261 tggtttccaa aaggcctgtt ggtcggcatg atggaaataa gattttcaaa tagacacaac
    85321 taagtatgta gaacgctttg tagaggactt tcggacgggg gttcgattcc ccccgcctcc
    85381 accatgccct attttcagca gtatcctgca acacaaaaaa tccttgaact tcaaaagttt
    85441 caaggctttt ttattgcctt gaatatgatg gcgtatgctt gagttttcgc aatttatggg
    85501 gtaacttttg gggtaacttt tcaggaatca ccctaaaaaa tatcttaaaa atacaaaagc
    85561 catcacgcat tttttaaaaa ggttacccaa aacttaaatg aagcctttat ttataaggct
    85621 tgcagctttc caaacaagtt acccccaaac ggataaaaaa tgccgaag
//
LOCUS       DAXFWM010000002        65588 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000002 DAXFWM010000000
VERSION     DAXFWM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 65588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65588
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.002"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            476..700
                     /locus_tag="ACFI8B_000091"
     CDS             476..700
                     /locus_tag="ACFI8B_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833418.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAATAMIAAHFIFVALSD"
     gene            756..1412
                     /locus_tag="ACFI8B_000092"
     CDS             756..1412
                     /locus_tag="ACFI8B_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3833419.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLEKLGKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIGGNTVLLADPSPGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVGQIRQGRAE
                     "
     gene            1540..2835
                     /gene="hisS"
                     /locus_tag="ACFI8B_000093"
     CDS             1540..2835
                     /gene="hisS"
                     /locus_tag="ACFI8B_000093"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC3833420.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVIQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            2836..3465
                     /locus_tag="ACFI8B_000094"
     CDS             2836..3465
                     /locus_tag="ACFI8B_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC3833421.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            3615..5072
                     /gene="der"
                     /locus_tag="ACFI8B_000095"
     CDS             3615..5072
                     /gene="der"
                     /locus_tag="ACFI8B_000095"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC3833422.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            5103..6002
                     /locus_tag="ACFI8B_000096"
     CDS             5103..6002
                     /locus_tag="ACFI8B_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC3833423.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            6054..6471
                     /locus_tag="ACFI8B_000097"
                     /pseudo
     CDS             6054..6471
                     /locus_tag="ACFI8B_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6538..7104
                     /gene="dcd"
                     /locus_tag="ACFI8B_000098"
     CDS             6538..7104
                     /gene="dcd"
                     /locus_tag="ACFI8B_000098"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC3833424.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7170..7874)
                     /locus_tag="ACFI8B_000099"
     CDS             complement(7170..7874)
                     /locus_tag="ACFI8B_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC3833425.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            8018..9213
                     /locus_tag="ACFI8B_000100"
                     /pseudo
     CDS             8018..9213
                     /locus_tag="ACFI8B_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            9239..10192
                     /locus_tag="ACFI8B_000101"
     CDS             9239..10192
                     /locus_tag="ACFI8B_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC3833426.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(10297..10620)
                     /locus_tag="ACFI8B_000102"
     CDS             complement(10297..10620)
                     /locus_tag="ACFI8B_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833427.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(10793..12151)
                     /locus_tag="ACFI8B_000103"
     CDS             complement(10793..12151)
                     /locus_tag="ACFI8B_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC3833428.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(12509..14209)
                     /gene="recJ"
                     /locus_tag="ACFI8B_000104"
     CDS             complement(12509..14209)
                     /gene="recJ"
                     /locus_tag="ACFI8B_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC3833429.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(14261..14992)
                     /locus_tag="ACFI8B_000105"
     CDS             complement(14261..14992)
                     /locus_tag="ACFI8B_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833430.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILVYAPLVDIGCG
                     GRGAGLAYGSDCSREFLRQMNGNAV"
     gene            complement(15039..15545)
                     /gene="yjgA"
                     /locus_tag="ACFI8B_000106"
     CDS             complement(15039..15545)
                     /gene="yjgA"
                     /locus_tag="ACFI8B_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC3833431.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RARVRLLADEGALTQFMSDFPNAGAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            15699..17030
                     /gene="pmbA"
                     /locus_tag="ACFI8B_000107"
     CDS             15699..17030
                     /gene="pmbA"
                     /locus_tag="ACFI8B_000107"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC3833432.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(17329..17532)
                     /locus_tag="ACFI8B_000108"
     CDS             complement(17329..17532)
                     /locus_tag="ACFI8B_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC3833433.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            17777..18079
                     /gene="clpS"
                     /locus_tag="ACFI8B_000109"
     CDS             17777..18079
                     /gene="clpS"
                     /locus_tag="ACFI8B_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC3833434.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            18081..20372
                     /gene="clpA"
                     /locus_tag="ACFI8B_000110"
     CDS             18081..20372
                     /gene="clpA"
                     /locus_tag="ACFI8B_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC3833435.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINGRFLPDKAIDVMDEAGAAQRILPKSKRKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(20412..23510)
                     /locus_tag="ACFI8B_000111"
     CDS             complement(20412..23510)
                     /locus_tag="ACFI8B_000111"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC3833436.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSGNSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNIVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(23742..24953)
                     /locus_tag="ACFI8B_000112"
     CDS             complement(23742..24953)
                     /locus_tag="ACFI8B_000112"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3833437.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFTELEATLEAELALRKRQYRYYRDLLLDFDNQIGGGIADGYQCRLKNVVWK
                     TLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSEGKMTEYIVNDILI
                     GNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLADDKFFAFNMKHAKGA
                     KMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSVSEGLPHEIALRRKQYEYY
                     REQLLAFPKAA"
     gene            complement(25120..25971)
                     /gene="dinD"
                     /locus_tag="ACFI8B_000113"
     CDS             complement(25120..25971)
                     /gene="dinD"
                     /locus_tag="ACFI8B_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFC3833438.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLRRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(26087..27631)
                     /locus_tag="ACFI8B_000114"
     CDS             complement(26087..27631)
                     /locus_tag="ACFI8B_000114"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC3833439.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIET"
     gene            complement(27706..28710)
                     /gene="rfaD"
                     /locus_tag="ACFI8B_000115"
     CDS             complement(27706..28710)
                     /gene="rfaD"
                     /locus_tag="ACFI8B_000115"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC3833440.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAAAVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(28774..29736)
                     /gene="hldA"
                     /locus_tag="ACFI8B_000116"
     CDS             complement(28774..29736)
                     /gene="hldA"
                     /locus_tag="ACFI8B_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC3833441.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDVIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(29792..30532)
                     /gene="pyrF"
                     /locus_tag="ACFI8B_000117"
     CDS             complement(29792..30532)
                     /gene="pyrF"
                     /locus_tag="ACFI8B_000117"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC3833442.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            complement(31056..31703)
                     /gene="adk"
                     /locus_tag="ACFI8B_000118"
     CDS             complement(31056..31703)
                     /gene="adk"
                     /locus_tag="ACFI8B_000118"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC3833443.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            31918..32757
                     /gene="htpX"
                     /locus_tag="ACFI8B_000119"
     CDS             31918..32757
                     /gene="htpX"
                     /locus_tag="ACFI8B_000119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC3833444.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(32993..34291)
                     /locus_tag="ACFI8B_000120"
     CDS             complement(32993..34291)
                     /locus_tag="ACFI8B_000120"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC3833445.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGCGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(34396..35547)
                     /locus_tag="ACFI8B_000121"
     CDS             complement(34396..35547)
                     /locus_tag="ACFI8B_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC3833446.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSAALLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVSVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            35884..37263
                     /locus_tag="ACFI8B_000122"
     CDS             35884..37263
                     /locus_tag="ACFI8B_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     NorM"
                     /protein_id="HFC3833447.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHKAV"
     gene            37307..38347
                     /gene="murB"
                     /locus_tag="ACFI8B_000123"
     CDS             37307..38347
                     /gene="murB"
                     /locus_tag="ACFI8B_000123"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC3833448.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSTEKAATLLQRHPDMPLYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPTSFSL"
     gene            38359..39009
                     /locus_tag="ACFI8B_000124"
     CDS             38359..39009
                     /locus_tag="ACFI8B_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC3833449.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            39064..39840
                     /locus_tag="ACFI8B_000125"
     CDS             39064..39840
                     /locus_tag="ACFI8B_000125"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3833450.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            39890..40456
                     /locus_tag="ACFI8B_000126"
     CDS             39890..40456
                     /locus_tag="ACFI8B_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC3833451.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIQKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            40476..41366
                     /locus_tag="ACFI8B_000127"
     CDS             40476..41366
                     /locus_tag="ACFI8B_000127"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC3833452.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            41391..42149
                     /locus_tag="ACFI8B_000128"
     CDS             41391..42149
                     /locus_tag="ACFI8B_000128"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC3833453.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(42204..42869)
                     /locus_tag="ACFI8B_000129"
     CDS             complement(42204..42869)
                     /locus_tag="ACFI8B_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC3833454.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSELWQFVVVQNPEIRQAIKLFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIVTKARRPLEETV
                     IWA"
     gene            complement(43066..43839)
                     /gene="folE2"
                     /locus_tag="ACFI8B_000130"
     CDS             complement(43066..43839)
                     /gene="folE2"
                     /locus_tag="ACFI8B_000130"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC3833455.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            44042..45199
                     /locus_tag="ACFI8B_000131"
     CDS             44042..45199
                     /locus_tag="ACFI8B_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC3833456.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(45263..46264)
                     /gene="hemB"
                     /locus_tag="ACFI8B_000132"
     CDS             complement(45263..46264)
                     /gene="hemB"
                     /locus_tag="ACFI8B_000132"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC3833457.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(46448..46732)
                     /gene="yhbY"
                     /locus_tag="ACFI8B_000133"
     CDS             complement(46448..46732)
                     /gene="yhbY"
                     /locus_tag="ACFI8B_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC3833458.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            46842..47462
                     /locus_tag="ACFI8B_000134"
     CDS             46842..47462
                     /locus_tag="ACFI8B_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC3833459.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KTIKPGTVLADLGSAPGSWSQVAAKLTGTSGTVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            47526..49493
                     /gene="ftsH"
                     /locus_tag="ACFI8B_000135"
     CDS             47526..49493
                     /gene="ftsH"
                     /locus_tag="ACFI8B_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC3833460.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIQTLLNKNVRVKVTPEEK
                     PSALTALFYSLLPVLLLIGAWFYFMRMQAGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            49715..50146
                     /locus_tag="ACFI8B_000136"
     CDS             49715..50146
                     /locus_tag="ACFI8B_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC3833461.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            50139..50417
                     /locus_tag="ACFI8B_000137"
     CDS             50139..50417
                     /locus_tag="ACFI8B_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC3833462.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            50470..51048
                     /gene="pth"
                     /locus_tag="ACFI8B_000138"
     CDS             50470..51048
                     /gene="pth"
                     /locus_tag="ACFI8B_000138"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC3833463.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            51109..51315
                     /locus_tag="ACFI8B_000139"
     CDS             51109..51315
                     /locus_tag="ACFI8B_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC3833464.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            51312..51674
                     /locus_tag="ACFI8B_000140"
     CDS             51312..51674
                     /locus_tag="ACFI8B_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC3833465.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFSVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            51903..53318
                     /locus_tag="ACFI8B_000141"
     CDS             51903..53318
                     /locus_tag="ACFI8B_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC3833466.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVKIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            53438..53947
                     /locus_tag="ACFI8B_000142"
     CDS             53438..53947
                     /locus_tag="ACFI8B_000142"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3833467.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            54115..55497
                     /locus_tag="ACFI8B_000143"
     CDS             54115..55497
                     /locus_tag="ACFI8B_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC3833468.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIALGRDGRLSGPELMEHIQRGLTDSGISVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(55553..56308)
                     /locus_tag="ACFI8B_000144"
     CDS             complement(55553..56308)
                     /locus_tag="ACFI8B_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3833469.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(56318..57034)
                     /locus_tag="ACFI8B_000145"
     CDS             complement(56318..57034)
                     /locus_tag="ACFI8B_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3833470.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(57024..57851)
                     /locus_tag="ACFI8B_000146"
     CDS             complement(57024..57851)
                     /locus_tag="ACFI8B_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFC3833471.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGDSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVAHNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAELVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            58136..59044
                     /locus_tag="ACFI8B_000147"
     CDS             58136..59044
                     /locus_tag="ACFI8B_000147"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC3833472.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            59121..62723
                     /gene="recB"
                     /locus_tag="ACFI8B_000148"
     CDS             59121..62723
                     /gene="recB"
                     /locus_tag="ACFI8B_000148"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC3833473.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPCLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEETTMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIARALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            62762..63121
                     /locus_tag="ACFI8B_000149"
     CDS             62762..63121
                     /locus_tag="ACFI8B_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC3833474.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            63246..63725
                     /locus_tag="ACFI8B_000150"
     CDS             63246..63725
                     /locus_tag="ACFI8B_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC3833475.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(64000..65379)
                     /gene="radA"
                     /locus_tag="ACFI8B_000151"
     CDS             complement(64000..65379)
                     /gene="radA"
                     /locus_tag="ACFI8B_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC3833476.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT        15434 a        18658 c        16577 g        14919 t
ORIGIN      
        1 ttattattga ttgcagaaac cattggaaaa acaatcaata agattaaata agatatttaa
       61 gccgctaaaa aaccatttga gtattttatc ttctatgcgg catccatctt ggaattgaaa
      121 tactgcaatc gggaggctaa ggcttccgtt gtaacgttta ccatatattt cattatttta
      181 atttcatcaa aaaacaaaat tcaagattat acataccgct aatcgtttct tgattgtttt
      241 gtatagtgga gtaaatttaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
      301 acggaaccga ttcactcggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
      361 gaggtaatgc cgtaccggtt taaatttaat ccactatata tttatccgat atatcaacat
      421 ctctctatct tattccgaat tttattgttg aaacttgcat taaaaaccat acaagatgat
      481 gaagaacaaa tatattttaa taaaagcagc aatcggcata accgccatat ccatatttta
      541 tcttctaact caagaaagca gcggcaaaac tgaagaacca tcatatttct tgatgtttat
      601 gtttctcaac ttgctttggt ttgaagaaaa taaaacagtt atggctgccg ctacggcaat
      661 gattgccgcc cactttatat ttgtcgcact atccgattaa taagcaaaca aaaaggctgc
      721 ttggaaattt caggcagcct ttcaggagga ttttgatgga acaaaaaagg cggtttgccg
      781 cttccctgct cctcgccgcc gccctgccgc tttgtgcaca ttcttttccc tttgcggaag
      841 aaaaccccat agcctacggc aaagtcaaaa tacaaagctg gaaagcgcgg cgggatttca
      901 atattgtaaa gcaggatttg gatttttcct gcggggcggc ttcggtggcg acgcttttga
      961 acaattttta cgggcaaacg ctgacggaag aagaagtgtt ggaaaaactg ggtaaggaac
     1021 agatgcgcgc gtcgtttgag gatatgcggc gcattatgcc cgatttgggt tttgaagcga
     1081 aaggctatgc cctgtctttc gagcagctcg cgcagttgaa aatccccgtc atcgtgtatc
     1141 tgaaataccg caaggatgat catttctcgg tattgcgcgg cataggcgga aatacggttt
     1201 tgcttgccga cccgtcgccg ggtcatgttt cgatgagcag ggcgcagttt ttggatgctt
     1261 ggcaaacccg tgagggtaat ttggcaggca agattttggc tgtcgtaccg aaaaaagccg
     1321 agacaatttc aaataaattg tttttcacac atcatcccaa gcggcagacg gagtttgcag
     1381 tcggacaaat caggcaaggg cgcgcagagt aagtcaaatg ccgtctgaaa actgtttcag
     1441 acggcatttt tacccgtccg caaaccatct ccatcctcac aagcccttaa aaatccgcta
     1501 taatcgcccg caatttgatt tcagcacccc ctttttgaaa tggcacaaaa aatccaatcc
     1561 gtcaaaggca tgaacgacct tctgcctgtc gggcaaaaag atttcaaact cacggctgtg
     1621 ttttggcagg cgtttgagga tacggtaaac cgttggacgc gagcttacgg ttatcagcaa
     1681 atccgtacgc cgattgtcga gcaaaccggt ttgtttgtcc gctccatcgg cgaggaaacc
     1741 gatgtagtcg gcaaggaaat gtacaccttc tccgattcaa acgattcttt gagtttgagc
     1801 ctgcgtcccg aaggtacggc ctcttgtctg cgtgcggtgg tcgaacacaa ccttctgtac
     1861 aacagcccgc aaaagctgtg gtatatgggg ccgatgttcc gccgcgagcg tccgcaaaaa
     1921 ggacgctacc gccaatttca tcaggtcggt atcgaggctt tgggttttga agggccggat
     1981 attgatgcgg aaatcatcgc aatgtctgcc gacttgtggg aaaaattggg tattcgcgaa
     2041 tacctgactt tggaaatcaa cagcttgggc aaccgtgagg aacgcgcggc acaccgtgcg
     2101 gcattggttg aatatctgac ccgctatgaa gcgcaactgg acgaagacag caaacgccgt
     2161 ctgaaaacca atcctttgcg cgttttggat acgaaaaacc ctgatttgca ggaaatctgc
     2221 aatgccgcgc cgcgtttggt agattacttg ggcgaggctt cgcaaaacca ctatgcacgc
     2281 ttcaaggcga tgttggacgg tttgggtatt caatatattg aaaattcgcg cttggttcgc
     2341 ggtttggatt attacaatca gacggttttt gagtggacga ccgacaaact cggcgcgcag
     2401 gcgactgtgt gcggcggcgg acgttacgac ggtttgattg aagaactcgg cggcaagcct
     2461 gcgccgtcca tcggctttgc gatgggcatc gaacggctgc tgcttttggt gagcgaatac
     2521 ggttcgctgg aagtgaacgc cgcgcctgat gtctatgcaa tgcaccaagg cgaaggggcg
     2581 gacttgcagg tgatgaaata cgcacaagcc ttgcgcgcgc aaggtttcaa tgtaatacag
     2641 cattccggct atcaaagcct gaaggcgcaa atgaaaaaag ccgacaacag cggcgcgcgc
     2701 tttgccctga ttgtcgcgca agacgaactg gcggacggta ccgttacgct caaagacatg
     2761 aacggcgcac acggtcagca aaccgtttcc gccaccgact tgacagatac tttacaacaa
     2821 tggaagaacg cataaatggc agcccatctc gaagaacaac aagagttaga caactttaaa
     2881 tatttttgga aaaccacggg caaatggctg tttgccctgc tgattttggc ggcactcggc
     2941 tacttgggat acacggttta ccaaaaccgt gcggcttccc aaaatcagga agcggcggcg
     3001 gtgctggcaa acatcgtgga aaaggcgcaa aacaaagccc cgcaaagcga aatcaatgcc
     3061 gaactgtcca aactccaaca aagctacccc cattccattt ccgccgccca agccacgctg
     3121 atggcggcgg caaccgaatt tgacgcgcag cgttacgatg ttgccgaagg tcatttgaaa
     3181 tgggtgttgt ccaaccaaaa agacagcctg attcaggcgt tggcggcgca gcgtctgggc
     3241 gttgtgttgt tgcaacaaaa aaaatacgat gccgcgcttg ccgcactcga cacgccggtt
     3301 gaggcggact tcgcccccct gctgatggaa actaaaggcg atgtttatgc cgcacaggaa
     3361 aaaagccagg aagccttaaa aaactacgga caggctttgg aaaaaatgcc tcaagattct
     3421 gtcggtcgcg aattgcttca aatgaaactc gattcgctga aataaatacc gtctgaaatg
     3481 ccgctctctc ttcagacggc atttcagaca accccatcct acggcaattc ccgtaaaacc
     3541 atccgccgtg tcccaacgga cacctgctcg tgttcagttc taccgaacgg cacttgacac
     3601 agaaagaccc aatcatgaaa ccaaccatcg cgcttatcgg ccgccccaac gtcggcaaat
     3661 ctaccttgtt caaccgtttg acgcgcacca aagacgcgct cgtgcatgac ctgccgggtc
     3721 tgacccgcga ccgccattac ggacacggca aagtcggcag caaaccttat tttgtcatcg
     3781 ataccggcgg tttcgagccg gttgtggaca gcggcatttt gcacgaaatg gcaaaacaaa
     3841 ccttgcaggc tgtcgatgaa gccgatgcgg ttgtgttttt ggtggacggc cgtaccggtt
     3901 taacaccgca agacaagatt attgccgacc gtttgcgcca aagtccgcgt cccgtttatt
     3961 tggccgtgaa taaaggcgag ggcggcgaca gggccgtact tgccgccgag ttttacgagc
     4021 tggcattggg cgagccgcac gttatttccg gcgcgcacgg cgacggcgtg tattacctga
     4081 ttgaagaaat tttagaaaac ttccccgagc ctgaagccga agaagccgat gcaaaacatc
     4141 ccgtttttgc cgttatcggt cgtccgaacg tcggcaaatc tacgctggtt aacgccattc
     4201 tcggcgaaaa acgcgtcatc gccttcgata tggcaggcac aacgcgcgac agcatccaca
     4261 tcgatttcga gcgcgaaggc aaaccgttta ccatcatcga taccgcaggc gtgcgccgtc
     4321 gcggcaaagt ggatgaagcg gtggaaaagt tctccgttat caaagccatg caggcggttg
     4381 aagcggcaaa cgttgctgtt ttggtattgg acgcgcaaca ggacattgcc gaccaagatg
     4441 cgacgattgc cggttttgct ttggaagcgg gacgcgcgct ggtagttgcc gtcaacaaat
     4501 gggacggcat cagcgaagag cggcgcgaac aagtgaaacg cgacatttcc cgcaaactgt
     4561 atttcctcga ttttgccaag ttccacttta tttccgcgtt gaaagagcgc ggtatagacg
     4621 gtttgtttga aagcattcaa gctgcctaca acgcggcgat gattaagatg ccgacgccga
     4681 aaatcacgcg cgtattgcaa accgccgtcg gacgccaaca gccgccgcgt gccggcttgg
     4741 tgcgtccgaa aatgcgttat gcccaccaag gcggcatgaa tccgccggtg attgtggtac
     4801 acggcaattc gctgcacgcg atttccgaca gctatacgcg ctatctgacc cagaccttcc
     4861 gcaaagcctt caacctgcaa ggcacgccgt tgcggattca atacaatgtt tcggaaaacc
     4921 cgtatgaaaa tgcggaagac aaaccgaaga aaaaaccgct gcgccgcgtc agcctgagca
     4981 accgtattga gaaacgcgaa ggccgaaagg aagagaaaaa ccgcttcaag aagaaaacca
     5041 aagtcagtgt gaaaaaacaa ttcagcaaat aattcccgac atttcaaaca ataggaaaca
     5101 ttatgtggtt caagcagatt agtttttatc cgctcaacaa agaaaagctg cctgaggcgg
     5161 acgtacttgc cgacaaactt gctgaagctg aatttaccca ttgccaaggt ttggactggt
     5221 tcagcgaagg ctttaccgca ccggtttctt tttcgcccga actcgttttc cctgccgact
     5281 ttaccttgcg cgtcgccctg aaaaaagagg aaaaagtcct gcctgccggc gtcatccgcg
     5341 atattttgga agagaaggta gcggaaatcc aaaacaatga agcccgcaat gtcggtcgca
     5401 aagaaaaaca agagcttaaa gagcaaatta cagacgacct gctgccccga gcgtttaccc
     5461 gcagcagccg tacagaagcg gtgtttaaca cccgccacgg ctacctgctc gtcaataacg
     5521 cggcttccgc caaagcggaa aacatcctga ccaagctgcg cgaagctttg ggcggtttgg
     5581 aagcctcgct gccgaatacc aaacaatcgc cctcttccct gatgaccggc tggctgttgc
     5641 aagggcattg cgaaggcggt tttgaattag acagcgattg cgaactcaaa ggtacgggcg
     5701 atattgttcc cgtcgtcaaa gtatccaaac aagacttaac cgccgacgaa gtggttcaac
     5761 acgtcaaaaa cggtaaaacc gttacccggc tcggtttggt ttggcgcgaa caaatcgcct
     5821 ttatcctcac tcaagacttc acactcaagc gcatccaata cctcgacgta ttgcaggaag
     5881 aagccgaaag caacggcgac gatgccgccg gccttgcctt cgcctcgcaa atcctgatgg
     5941 cggaatccgt cagcaccatg ttggaagagc tggtttccta tttgggcggc tggcaagatt
     6001 gatttttcag acggctttta taccgtcagc caaaatccaa tggaataacc cttatgctta
     6061 aacatctcgc attcctactg cccgccatga tgttcgccct ccccgcccag accgccgtcc
     6121 taagccccta tcaggaaacc ggctgcacct acgaaggcgg gatcggaaaa gacgggcttc
     6181 cttcaggcaa aggcatatgg cgttgccggg atgggcgcgg ttataccggt tcattcaaaa
     6241 acggcaaatt cgacgggcaa ggcgtttata ccgttgccgc cggccgcgaa gtatttctcg
     6301 agccgttcaa ttccgacagt accaaattcc gcaatatggc attgtcgggc acgttcaaac
     6361 aaggcttggc acacggcagg ttcgccgcct cgcaaaacgg cgaaaccctc ttttattatg
     6421 aaatgcgaac acggcatgat taaagaagtg aaactgccca aaaacaaata accccccgtt
     6481 tttcaaacag catcccccaa tgctttatcc gaacaccgca aaaaacaagg aaaccccatg
     6541 agcatcaagt ccgacaaatg gatacgccga atgagcgaag aattcggcat gattgatccc
     6601 ttcgagccga accaaatcaa agaagccgac ggacagcgca tcatctccta cggtacgtcc
     6661 agctacggct acgatatccg ctgcgccaat gaatttaaaa tttttaccaa catcaacagc
     6721 accatcgtcg atcccaaaaa cttcgacccg aaaaatttcg ttaccgtcga agacgactgc
     6781 tgcatcatcc cgcccaattc cttcgcactg gcgcgcacgg tcgaatattt ccgcattccg
     6841 cgcaacgtcc tgaccgtctg tttgggcaaa tccacctacg cccgctgcgg catcatcgtc
     6901 aacgttaccc cgttcgagcc ggaatgggaa ggctacgtta ccctcgagtt ttccaacacc
     6961 acccccctgc ccgccaaaat ctacgcaggc gaaggcgtgg cgcaagtcct cttcttcgag
     7021 agcgacgaag tgtgtgaaac ttcatacaaa gaccgcaacg gcaaatacat gggacaaacc
     7081 ggcgtaaccc tgcccaaagc ctgaaataca aaatgccgtc tgaaccctta tcgtcgggtt
     7141 tcagacggca tctacctatt tatccgtggt tagaactgaa ccgtcccatt gatgctgatg
     7201 ctgatttcct ccacacccgg tgcggctgaa tccgtaccct tcatattgac gcttgccgcc
     7261 atcggcatcg cgcgcagcat ttttgcccga acagccccat cgcccgcaat atggctgccg
     7321 atttgcccaa aattcaattt gacgatttta taaccggacg cacccagaac gcccgccagt
     7381 ttttcggcac gcgccttgaa acgcaaaacg gcatccttgc tgacctgatc gatgacctcg
     7441 tttcggcgtt cgcgcgacac gctgaaatcg gtatcttcaa gcgaagcatc cgtctgaaca
     7501 tcagcaataa aacggtttaa agcatcaaaa tccctgccct ccgccttaaa ttccgcacgc
     7561 tcctcccagc ctgtttgaat gcgtctgccg ttggtatatt gatagcgcgg catcgcactg
     7621 cgcgatacca attcggtttt aaagctacca tttttcgatt ttctggtaaa attgttgaat
     7681 tttttaacaa actcggcatt gacggcattt ttgtcccgtc cttccgccgc cacctggaaa
     7741 cgcgcggaca ttgtatcctg agccacctcg atacccgccg attcggaaaa ttcgacaata
     7801 ttgtaattca atgcctcagc cgccgccgga aaagataccg ccagcaggga agccgccaaa
     7861 acagaacgca gcatatccgt atccttactt gaaaaaccgc caatctaaca tataggtacg
     7921 ctaaattgcg ttagcataag acaacaattt aaatattcga tcggtctgcc ttataataac
     7981 cgtctttccg atactgaatc atattatgtc cgaaccgttg cacgtcctcg tcatcccctt
     8041 atggtatcca cgatccgaac aggatgtgga cggaatattt ttccaaaatc aagcacaggc
     8101 cctccaacgc aaaggcatca aaaccgccat cctcgccccc atgttccgtt atctgcggaa
     8161 agaaacagca agcatcctga ccggtcctta cggttttgcc cgataccggc aaagcggttt
     8221 ggacatctac gcatggcgca gcatgtattt cttccccgct ttccgtttat cgacatcgac
     8281 cgcatccgct gggtgcgcgc cggtttgaaa gccttcaaac actacatccg cgaaaacgga
     8341 ctgcccgacc tgattcacgc ccactgcatg aactatgcag gcatacttgc ccaaaaaatt
     8401 tccgaaaaat acggcattcc ctacgtttta accgaacaca gcagcaccat cacgcgcggt
     8461 ctgatccgtc accaccaatg gcagccgatg gaaaaagcag cggcgcccgc ctccgcacgt
     8521 ctcgccgtca gccgccattt cgcacacgtc ctgcaacaca aatacggctg cgaatggcag
     8581 tacctcccca acatactggg tggaatattc aagcaaacat ttgaataaaa agaaaaaaat
     8641 aacaaaccgc atttcgtgtt ctgcaccgtc tcgcaccttc gccgcctcaa aggacacgat
     8701 gtcctgctca ctgcttttgc ccgggcgttg gcacaatgcc cgcaactgcg cctgaacatc
     8761 ggcggcagcg gacaggaaga acagcggctg aaacagcagg cggcagactt gggcatcgcc
     8821 catgccgtta catttttagg cgcactgcaa cccgaagcaa tcttggattt gatgaggaac
     8881 agcgacgcct tcatcctcgc cagccgcacc gaaaccttcg gcgtggtcta tatcgaagcc
     8941 ctctcgcaag ggttgcccgt catcgccata cattgcggcg gcgcggaatc catcgtttca
     9001 gacggcaacg gatacctcgt ttccgttgac gatgccgatg tcctagcctt tctcaaaata
     9061 tatgaacacc atgccgattt ttccgccgaa cagctcagaa ccgactgcct cgcaacattc
     9121 ggcgaagatg ccgttatcgg cagactgatt gccgtattcc gtcaggcggc gcggaacacg
     9181 gcgcgaaaac gcccgaaaaa tagattaaaa tgacgcaaaa caaccttcgg attcaaatat
     9241 gacccatacc gtccacctgc acttggaaga aaccgacaac ccgacgctgc aacgcctgtg
     9301 cggttctttc gacagcaacc tcgattcgct cgccaaagca ctcgacatcc atatcagccg
     9361 ccgtttcgag cattttacct tcaacggtgc atttgcacac gccggcaaac gcgcactgct
     9421 caaactcttg gaaacggcgc agacgcgcga cctaaacgac agcatcatcc gccttgccgc
     9481 cgtcgaaacc caaaccgaag atgccgggca tcaggaaaaa aatcacgaac acgcctatta
     9541 tttccgaacc aagcgcggca gcataggcgg cagaacgcca cggcagaacg gctatatccg
     9601 cgccctgctc gaccacgaca tcgtattcgg cctcgggccg gcaggtacgg gcaaaaccta
     9661 tcttgccgtc gccgccgccg tcgatgcgat ggaaaaacac caagtcgaac gcatcatttt
     9721 agtgcggcct gccgtcgaag ccggtgagaa actgggcttc ctgcccggag acctgaccca
     9781 aaaagtcgac ccctacctcc gtccgcttta tgatgccctc tatgacctga tgggctttga
     9841 ccgtgtaacc aagctgattg aaaaaggcct gattgaaatc gccccgctcg cctatatgcg
     9901 cggcaggacg ctcaacggcg catacatcat cctcgacgaa gcgcaaaaca ccacgcccga
     9961 acaaatgaaa atgttcctga cccgcatcgg cttcggcgcg aaagccgtca ttaccggcga
    10021 caccagccaa atcgacctgc ccaaaaacat caaatcggga ttgaaagacg cacgtgagaa
    10081 actgcacggc gtggcaggac tgtatttcca taccttcacc ggcgaagacg ttgtccggca
    10141 tcctctggtg caaaaaatcg tcgaagccta cgaatcggca gaacacgact gacactccaa
    10201 tgccgtctga aacccgatgc atggaaaatc gggcagagaa aaacagagac ggaagccaag
    10261 ctccgtatcc gtttctttat ccgtccaacc gatacatcaa tcgaattttc tgcatcggat
    10321 tgcctcatct ccgcaaaaca ttgccgaagt ttcgcggttg cacgcctgac gcactttctg
    10381 ccgcgccacg ccttcattcc gatcgcttgc ctcaaacgtc ttctgaaaag gcgtaacgct
    10441 gcatacatac atggtggaat ttgaaccgaa aaggtcgatt ttgacggtat tgccgccgct
    10501 ttccaatacg gcgacacggt gttccaactg cgccagacgt gcttccaaat cgctgattct
    10561 gtcggcaaat gcaggcatgg cggcacacgc ggtcaaaatg cccaaaatac attttttcat
    10621 caatttttcc ttgttgcttg aaccctgccc acccggacat cctttttccg ggcggcaggg
    10681 tcagacttta tttgcaggga tgaagcccct tacaaatcag aataatacat gaatcggcac
    10741 tttatgcttc atcctatgtg ttgaaacttc attgctcctt gtctgttgac cgttattccc
    10801 aatccgttcc cacaaccttc ggcttgggtt tgcgcctgcg acggcggcgt tttttcgcct
    10861 gtccttcgtt tttttcgtgc cgggcggcgg cctcgttttt ggtcatttcc gtccgctgtt
    10921 ccgccgatgc cgtctgaaac gccgtccacc actcggcaag tgcgcggtcc gcattgccgg
    10981 tttcggcgcg caagagcagg aaatcatagg cggcacggaa acgcgcctgt gcaaacagtt
    11041 tgtgcggacg tgcgcctttg cggttttcaa actgcggctg gaacatccaa atttcacgca
    11101 tcgtggcgga aaagcgttgc ggtacgcccc aaccgcgttc gacggtttcg cgcatcgtat
    11161 tgattgcatc ggacagggcg ggcgtaggtt tcaaaccctg ttgcagattg cttttccaat
    11221 ggcggtccaa ctctggccac atcagggccg ccagtacgaa accgaccgaa accgatttgt
    11281 cggcacgcag ccgctcatcg gtatttttca gggcaagcgc cgtcattttt ccggcgatgc
    11341 cgtctgaaac gcgcaaagca ttgagcagcg gatggatgtc cggtatgtca aatccgttca
    11401 aacgtttcag acactcgcgc gcgtgccctg aaaacagcag tttcatgatt tcgtcgaaca
    11461 gccttgccac aggttcgtgc ttcagacggc atatcgattc ggcaatcggt gcggcggttt
    11521 cttccgacag ctcaaagccc aatttgcccg acaggcggat ggcgcgcaaa atcctgacag
    11581 ggtcttcctg atagcgttcg gcggcattac caatcataac cagcctgcgg gcggcaacat
    11641 cggcaatccc gttgtggaaa tccaaaatct cttctttttc aggatcgtaa tacaaggcat
    11701 tgcaggtaaa atcgcgccgc atcgcatctt cttcgatgct gccgtaggtg ttgtccttca
    11761 tgattctgcc gtgcgcgttc tgatgaacct tcgcaccgcc gcgaaacgtc gttacttcga
    11821 taatctccgc accgttcatc acatggacaa tctgaaaacg cctgccgatg atgcggctgc
    11881 ggcggaagag tttgtgcacc tgttcgggcg tggcatcagt tgcgacatcg aaatctttgg
    11941 gttcgatgcc gagcagcagg tctctgaccg caccgccgac cacataagcc tgaaaccccg
    12001 cccctttcag gcggcgtatg acgttttcgg cggcaaagct caacatttcg gcacggatgt
    12061 tgtgtctttc ggcaggaatg accgttttac tttccgcttt tttactgctc cgaccggaag
    12121 gcagcatctt attcaaccat tttttcagca taaatattct gacccgaaga gcggcatctc
    12181 cacgggcaac cgtgttcaga ctgcatcagg cggtacgatt ccacaagatg ccgaaaacca
    12241 taagcaaaag gcgtagaaac tacgccgtcc gaatcacgcc gccctcgcgg cggcaacgcg
    12301 tcattataac agattgcccc ttaaaaaatc agaccctgct tttgtggcgg agtctgaaat
    12361 ttgatgcacc ttatatattg cgtccctaag aagggatgat taacaaaaat taacaccctt
    12421 tactttcttt tccgattggg aacattcccg tcgtacaaac aagcaaatga aggaacagaa
    12481 atcacattca aacggcgttc cgccgcctct acgccgcttc ccagtagtcg atataaagct
    12541 gcaattcgag gttgtcgcgc cattcgttgg caacggggcg gtacaccgtg cggatgtatt
    12601 cgggaatgtc ttcgctgcaa cgccagaaca tcgcttcaaa ttcgcagccg tctttttgca
    12661 accacgcttt tttgtgtttg ccttccgcgc ccaaaggctg ctggcgaacg acgtggaact
    12721 cgtcggtaaa gctcggcggc gcaaagccct gcccccaaac gtggcaggcg aggttttgcg
    12781 cctgttccaa cgtgatgtcg caggcgggca ggctgccgtc agtaatatag gtttgcgaca
    12841 aatcgtcttc gcacaccatc tcacgcacgg cttcttcaaa ggccgtctga aacgcgggaa
    12901 tattgtgttc aagtatgctt aaacccgccg ccatcgcgtg tccgccgaat ttcaaaatca
    12961 aatcgggatg gcgtttggac accaagtcca aagcatcgcg caagtgcaga ttgggaatgg
    13021 agcgtcccga accgcgcact tcgccgttgt ccgccggcgc aaacacgatg gtcggacggt
    13081 aaaaacggtc tttgaggcgg ctggcaacaa tgccgaccac gccttgatgg aagtcgtcgc
    13141 gatacgccac caaagtcgtc tgacctgaag gcagggtttc gggaaaggcg ttcaacgcgt
    13201 ctcgcagcat agactgctcg atttcgcggc gttcgatatt gaggttgttt aaccgagctg
    13261 ccagttcctg cgcttcggaa tcatctcgcg ccaacaggca ggcgatgccg accgacatat
    13321 cgtccagccg tccggcggcg ttgatgcgcg ggcccaacgc aaagcccata tcaaacggct
    13381 gagccttgcg ccaatcgcgc cgcgccactt caaacaaggc gcggatgccg gggcgcattt
    13441 tgcccgaacg catccgtttc aaaccttgcg acacgaggat gcggttgttg tggtcgaggg
    13501 ggacgacatc ggcgacggta ccgagtgcga ccaaatccaa aaggccgccc agattcggct
    13561 ctttgatgcc gtctgaaaaa taattgcggc ggcgcagttc ggcacgcaac gccgtcaaca
    13621 cataaaaaat cacgcccacg cccgccaagc ttttgcttgg aaaaccgcag cctttttggt
    13681 tcggattgac gatgatgcag tcgggtacgg tatcggctgg caaatggtgg tcggtaacga
    13741 tgacatccaa acccagagcc tgcgcgcgcg ccacgcctgc aatactggca atgccgttat
    13801 ccaccgtaat cagcaaatcc acgccttgcg cggcggcgat ttcggcgagt tcgggcgtta
    13861 agccgtagcc gtgctcaaag cggttgggca cgaggaaatc cactttcgcg cccatcgccg
    13921 ccaaaccgtc caaaccgacg gcacacgcgg tcgcgccgtc ggcatcatag tcggcgacaa
    13981 tcaggatttt ttccttgcgt tcaaccgcat ccgccaaacg gccggcggcg gcttcgcaat
    14041 tcgtcagcga ttgataaggc aggagggaag cgagtttgtc gtccaactcg gcaggacttt
    14101 gcacaccgcg cgaggcacaa agccgggcga ttaaaggatc ggcgccggcg gcgagcaaat
    14161 gattgaaaac gtcggtattg acggggcggg tttggatttt gactgacata gcgcggggtt
    14221 ttacagtgcg ggtgtgcttg aaatgccgtc tgaaggcttt tcagacggca ttgccgttca
    14281 tttgtctgag gaattcgcgg ctgcaatcag agccataggc aagaccagca ccgcggccgc
    14341 cgcatccaat atcaacaagg ggggcgtata ctaagatatt tccaaacagc ttggatttgt
    14401 cggtatgttt ttcagtaacc gtataataaa tatcggcggg cacactttgc tcaaaatgat
    14461 aatcggcgtt cagtttttgc ggtgtggcgt agtatttgcc tttggcggat acgcagcgcg
    14521 tgtaaatggt ccgattgtcg agtttgaccg ctttaaactc aagctgtttc agcttggcga
    14581 tgtcgtcagg tctgccggta tcatagcgca ggcaaagacc tccggtactg aaattctggc
    14641 tgccgggcgc ttcgaatttg accggcaggg cttggtggcg ggcatagctc ggggtatcct
    14701 caactatttg gaagggcttg tccaacccgg ccttcaaaag gcccgtcagc ttcgccgaat
    14761 cttcgggatt gacggcgaac cagtatttcc cgcccatcat caccaggctg cccttttcca
    14821 attgggcatt gtcttcggca accacaccga aggcgcggat ttggtctttg tcaacgtgtt
    14881 tgcgggtgat tgtttggctg accgggttgt tcatcccccg caacatcatc gtacagccgt
    14941 tcagtatcaa ggttgccgcc aaaacggcgg cggtcgtttt acggaacaac attttatctt
    15001 ccaatctaaa taacggcgga tgccgtctga aaatatgcct aaatttccgc gcccccgttt
    15061 tccatcacgg ttttcaattc ttgaaacagg gcgcggaagt ttttcggcgg tttgtcctgt
    15121 tcctgttctt ttttggtgtt gcggacgagc gtcctcagcc tgcccgcgcc ggcgtttgga
    15181 aaatcggaca taaactgcgt cagcgcgccc tcgtctgcca acaaccgtac acgcgcccgt
    15241 tccacgcgtt gcaaaaaggc gttgtgcgcc gcatcgtcgc cgcgcagctt ggcaaggaac
    15301 gcctcgatgg gcgcgggatc ggtatcgcgc atcagacgtc cgataaattg cgcctgacgt
    15361 ttgagcgcgc cgttggacgt aattttttta taggtaacga cggcctcgta caaatcttca
    15421 tccaaaccga tttttttcag cgtgtcgttt gagagcctgg tcagttccat acccaaatcc
    15481 tgcaaaccgt tcatctgctt tttcatttgg gttttgctga tccactcgtc ttcttgttca
    15541 aacatcttga ttctcgaatt ttttccaata gcgtatttta acagaatgcc gccgcgttca
    15601 gacggctttt tgcaccgcac gccctgcaag cggcacgctt ttgcccctgc cgcccgaaac
    15661 ggttaaaatg ccgcaacgct ccaccaagaa agattgttat gctgttcaac cacaccgctt
    15721 ccgaactcct cgacctctgc cgccgcacgc tcgacttggc aaaagcagcg ggcgcgaccg
    15781 ccgccgaagc cgacttcagc gaatcgctcg gacaaagcgt cagcgtgcgc ttgggcgaaa
    15841 tcgaacaaat cgagttccag caggacaaat cgctggacat taccgtttac gtcggcaaac
    15901 gcaaaggccg cgccggcacc gccgacttct ccgaacaagc cctgcgcgac accgtccgcg
    15961 ccgccgccga catcgcccgc cacaccgccg aagacggttg cgccggactt gccgaccccg
    16021 agttgatggc gcaacacatc ggcgaccccg acctttacca cgaatgggat ttggatacgg
    16081 aagccgccgt cggcttggca aaacaatgcg aacaggcagc gttggacacg gacagccgca
    16141 tcgaaaactc cgaaggcgcg gcggtgcaaa ccggacacta ccaatacgtt tacggcaaca
    16201 cccacggttt tgccgcacac cggcaaagca cgcaccacag catttcctgc agcgtcgttg
    16261 ccgccgacga aaacggaatg cagcgcgact actggtacga ttccgcctgc cgccatccgg
    16321 atatggacag ccccgaaacc atcggtcaaa ccgccgcccg ccgcactttg cgccgactcg
    16381 gcagccgcag catcccgaca ggcagctacc ccgtcctctt cgataccacc gtttcgggcg
    16441 ggctgatcgg acacctcgtc ggcacactct ccggcggcgc gctctaccgc caaagcagtt
    16501 tcctgatcga cagcatcggc aaaaaagtcc tgcccgattt cctcaacctg cgcgaagaac
    16561 cgcacatccc ccgctctttc cgcagcagct attttgatgc ggaaggtgtt gccaccgcgc
    16621 cgcgcttcgt gattcaaaac ggcattgtcg aaggctattt cctcggcagt tacagcgcgc
    16681 gcaaactcgg aatgcagacc acgggcaacg ccggcggcgc gcacaacctg tatttgaacc
    16741 atacgcacga aacgcaatcc gacctgctga aagaaatggg cacgggattg ctggtaaccg
    16801 aattgatggg acagggtgcg aacaccatca ccggcgacta ttcgcgcggc gcggcgggtt
    16861 tttgggtgga aaacggcgtg attgcctacc ccgtccacga gattaccgtt gccggacgct
    16921 tgcaggatat gtaccgcgac atcgtcggcg tggcggatga cgctttgcgg cgttcgtcca
    16981 acaaaatcgg ttcgattctg attgcgggga tgacggttgc cggaagctga acgccctgcc
    17041 gatgccgtct gaagacgaag gcggcgcgtt ttgccccgtg cctgcccgtt tgccgtttta
    17101 tgccgcaccg ccttttaccg gcgacattgc acattttttt catcagcact gattttccct
    17161 tatccgtcaa aatactaagc cacttgccca caacagggta cacacaagtt caggataagg
    17221 cgggcgggga ttggggactt ttccgccatc ggcaaaacgg cagacaaaaa cggcgggaca
    17281 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagctgcctg
    17341 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
    17401 ttctttcagg gttttgaaac cttccatatt gatcgctgag aagtgtgcga acaaatcttc
    17461 gccgccttcg tcaggagtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
    17521 accggttgcc attagaaact tcctatactt aaaaattaac aaaatcagca aaataaggca
    17581 tacagcaaac ttaaacgttc agcgtttcca cccagcttta tatacttggt cgagtgccga
    17641 cttttgctta accgtctttt tacatttgtt aagacaaaac gtcaagctat gttttcaaaa
    17701 aagtgggaaa ataggcgaaa gtaccgaaaa tacaacaaaa atgccgtctg aacgcggctt
    17761 tcaaatggga cacgctatga ccgcgcaaca ccaatccgac acgcttttgc accgcctgaa
    17821 caccctgccg ccgaaacgtt acggcgtatt tttattgaac gacgattaca ccacgatgga
    17881 atttgtcgtt gaaatcctga ccgaaatctt tatgctcggg caggaacagg cggtagcggt
    17941 aatgctctcg gttcatcacg aaggcaaagg cctgtgcggc acttacacgc gcgatatcgc
    18001 ccaaaccaaa cagcaacaag tcatgcagcg ggcaaaagcc gaaggacatc cgctgcaatg
    18061 tattgtcgag gagatttaat atgcttgccc ctgaattgga acagattttg cagcagcttt
    18121 accgcgaggc gcgtaaggct cattatgaat ttatcagcct cgagcatctg cttttggtac
    18181 tcatcgaaga agatgccgcc gtgccgaacg tcttaaaact ctgcggtgcg gatttaaaag
    18241 cggtgtccga acaactcgcc gccagcgttg ccgaaaacac ccccctgatt cccgaccacc
    18301 ttttagacac ggtcgaaacc cggcccacgc tcggcttcca acgcgtcatc cagcgggcga
    18361 tggtgcatac ccaatcggca ggcaaaggct tggccgagcc tttggacgtt ttggtcgcgc
    18421 tgatgagcga aaccgacagc cacgccgtct atttcctcaa gctgcaatcg gttacgcgtt
    18481 ttgaagtttt gcgctgtatt gcccacggct ctcccgatga agatgaagac gatggcaact
    18541 atttttcaga cggcatggac gacgataatg agaaccgcac caaaccgggc aaaaaccctt
    18601 tatcggcgta caccgtcaac ctcaacgccg aagtcaaagc cggccgtatc gatcctttga
    18661 ttggtcgcaa acacgaaatg gaacggctgg tgcaaatcct gtgccgccgc cgcaaaaaca
    18721 atccgctttt ggtcggcgaa gcaggcgtgg gcaaaaccgc gctggcggac ggtttggcac
    18781 atcaaatcgt caacgacgac attcccgacg cgctcaaaga ggccgaagtg tacgcgctgg
    18841 atatgggttc gcttttggcg ggcacaaaat accgcggcga ctttgaagcg cgggtcaaat
    18901 ccgtcttgaa acagctcgaa aaaatcccgc acgccatttt attcatcgac gaaatccaca
    18961 ccatcatcgg cgcgggcagc accggcggcg gcacgatgga cgcgtccaac ctgctcaaac
    19021 ccgcattggc gaaaggttca ttgcgctgta tcggcgcaac cacttacgac gaataccgta
    19081 ctatttttga caaagaccac gccctaagcc gccgtttcca gaaaatcgac gtggtcgaac
    19141 ccaccgttgc cgaaaccgtt caaatcctgc gcggcttgaa accgatgttc gaagccttcc
    19201 accaagtccg ctacacgcaa ggcgcactcg aagccgccgc cgaactctcc gcccgctaca
    19261 tcaacgggcg cttcctgccc gacaaagcca tcgacgtaat ggacgaagca ggcgcggcgc
    19321 aacggattct gcccaaatcc aaacggaaaa aagtcatcgg caaagcgcaa atcgaaaccg
    19381 tcatcgccaa agtcgcgcgg attcccgaaa agaccgtgtc gcacgacgac aaacaagtgt
    19441 tgcaattcct cggccgcgat ttgaacaata tggtttacgg gcaggaagac gccatcgacg
    19501 cgctggtttc cgccgtcaaa atgtcgcgtt cgggcttggg gctgcccgac aaaccgatag
    19561 gcagcttcct cttctccggc cctaccggcg tcggcaaaac cgaagtcgcc aaacagcttg
    19621 cctactcaat gggcgtaccg ctgcaacgct ttgatatgtc cgaatacatg gaacgccacg
    19681 ccgtatcgcg actcatcggc gcgccaccgg gctacgtcgg ctttgaacaa ggcggccttt
    19741 tgaccgaagc cgtcaacaag cagcctcatt gcgtgttgct cttggacgaa atcgaaaaag
    19801 cccatccaga tattttcaac gtcctcctgc aagtcatgga cgcaggcaaa ctgaccgata
    19861 acaacggtaa gagcgccgat ttccgcaacg tcatcctgat catgaccacc aacgcaggcg
    19921 cagaaagcct cagccgaccc agcctaggct ttaccgccaa acgcgagcgc ggcgacgaaa
    19981 tgcaggcaat caacaaactc ttcacgcccg agttccgcaa ccgcttggac gcgatcatcc
    20041 cgtttgcacc tttgtccgaa cccatcatcg tcaaagtcgt ggacaaattc ctgctccagc
    20101 tcgaacaccg gctcctcgac aaaaaagtcg aagccgaatt cacaccggca ttgcgtaaat
    20161 acttggcaga aaaaggtttc gacccgcaaa tgggcgcgcg cccgatgcac cgcctgattc
    20221 aggaaaaaat ccgcaaaccg ctcgccgacg aactcctgtt cggcaaactc gccgacggcg
    20281 gctttgtgcg gatagactgg gatgcggcga aagaagaagc cgtattgaag tttaagaaaa
    20341 gcaaggtcaa aataaaaacc gcctccgcat aaaatcaaaa atgccgtctg aaaggttcag
    20401 acggcatttt gtcaaatatc ggcccctatt ccggcaaact tctccacaaa cgccgcgatt
    20461 ttttggaaga cgctttgttt cttcgtcaga tattgcgggt tgagcggact cattttcggc
    20521 agggtttcgg tcagttccgt gccgttttcg ctggcatagc cgcgtttgag cgagccggtc
    20581 aaatagcgtt tggcggcggt ttcgttcagg ccttcggcgg caatcaatcc tgccgcttcg
    20641 tgccgcatca cctcttgcgc gtaggtgtaa aaggtttcca gtatggcggg aacgtcgggc
    20701 actttatcca aatccgtatc gttgatgaaa tccacaatca ggccctcttt ggcgcggtgg
    20761 ccgatgctgg cgcggatgat gcggcggatt tcttccacca gctccgcttt gcctttgatt
    20821 tttttgtgat gctcgaaaac cagttgcagg atgtaatcca gattgatttc ctgcgatttg
    20881 agcaaatcca cctcaaaaac cacatcgtcc cagtcgattt ttgactgttc tttctgttcg
    20941 cctgcttttt ggcggcgcag ccagtctcgg atgtcgttat aggcggaacg gtagtcctgc
    21001 accgcccttt cagacggcat cggcactttc cgcatttcct gcacgtcttc gtcgctcagg
    21061 tagtattttt cctgaaacgc cttcatcgca tcttcgtccg ccgcgtccac attctgcaat
    21121 tcgcgcagcg cggcaaattc atcgtagttc tgcaatacgt tttccgcccg caggtattcg
    21181 ccgaagagtt tggcaaaatc ttttttgtct ttttccgttt cgattttgtc gggatcgggg
    21241 aaacgctcgt gcaattcttt tgctacatcc agataaccgc gccgtgcttc gccggtctgg
    21301 ctgtcggtat agccgttcat gtattcttcg tagctttttt ccagcactat atttttggtg
    21361 tttttgtcgc cgaagagcgt gatcgcatca atggttgcct gctccaagtc gcggaagcag
    21421 acaatgttgc cgaaggtttt ggtagcatcg taaatgcggt tggtgcgaga aaacgcctgc
    21481 atcaggccgt gatagcgcag gtttttatcg acgaacagcg tattcagcgt cggtgcgtca
    21541 aaacccgtca aaaacatgcc gaccacaatc agcaagtcca cttcccggtt tttcacccgt
    21601 tttgccaaat cgcggtagta gttctgaaag gctttgctgt ccgtgccgaa attggttttg
    21661 aaacaggcgt tgtaatcgtt gatggcagcc tgcaaaaatt ctttcgcgct gctgtccatc
    21721 gcttccggtt caaaggtctc gtcaacaatt tcaccgacgg cgttttgctc ttcgttggcg
    21781 gcaaaggaaa aaatggtggc tgttttcaac gggtgcaggc tgcctgcctg ttgtgttttg
    21841 aacgcttcgt aatagcactt cgccgcatcc acgctgctga cggcaaacat ggcgttgaag
    21901 cctttgccgc ccgcattcaa gcggtgcgtt ttctgcctga accggctcag gatatattgc
    21961 gtgatttcgc ggatgcgttc ggggtgcagc agggcttggc ggttttcggc ggcactcagt
    22021 tccttctcgt cctgttccgc ttccacggct ttgaactgcg ggcgcacatc gttgtaatcc
    22081 actttgaatt tcaatacttt ttcatcgcgg atggcatcgg taatcacata agaatgcagc
    22141 tcccgcccga acacgcccgc cgtggtttcc gcgcccaaag cgttttcggg aaaaatcggc
    22201 gtgccggtaa agccgaactg gcagaatttt ttaaatttct ttttcaggtt tttttgcgct
    22261 tcgccgaatt gcgagcggtg gcattcgtcg aaaataaata caacctgccg gtgataaacc
    22321 ggcagattat cttcactttt catcaggttg ttcagctttt ggatggtggt aacgatgatt
    22381 ttgttgccgt ctttttccaa attgcgtttc aagcccgccg tactttccga gccgtttacg
    22441 ctgtcgggcg aaaaacgttg gtattccttc atcgtctgat agtccaaatc cttcctgtcc
    22501 accacgaaga aaaccttgtc gataaacgcc gattccgtcg ccagacgcgc cgccttaaag
    22561 ccggtcagcg ttttgccgct gcccgtggta tgccaaacat agccgccgct ttccggcccg
    22621 ctccaattct tcgcctgcgc cgagctgttg attttccaca aaatgcgttc ggcggcggca
    22681 atctggtacg gccgcataat cagcagcgta tcattcgcat cgaacacgct gtaatgcagc
    22741 aaaacgctca gcaatacgct tttttgcagg aacgtggcgg taaagtcttt caaatcctta
    22801 atcggatgat tgtccgaccg cgcccaattc atcgtgaaat cgaagctgtt tttgtcgcgc
    22861 ttggtggtgt tggcgaaata acgcgtgtcc gtgccgttgg aaatcacgaa gatttgcagg
    22921 aatttgaaca gcgaatttcc gctgttgaag ctctctttgc tgtaacggtg cacctgattg
    22981 aatgcctcgc gcaccgccac accgcgcttt ttcaattcaa tctgcaccag cggcaggccg
    23041 tttaccaaca cggtaacgtc ataacggttt gcatgcgtgc ccgtctgctc aaactggttg
    23101 ataagctgca catggttgcg ggcaaggttt ttcttgtcca gcagataaat gtttttcaga
    23161 cggccgtcat caaaagcgaa atcgtaaata tggtcgtcgt ggattttgcg ggttttatcg
    23221 gtaatgtttt cagccggcct gtccagatat tccgtcaaaa accgcgccca ttcgccgtct
    23281 gaaaacgcca catcgttcag ccgctgcaac tgggcgcgca ggttttccag cagcctgctt
    23341 tggctgttca aatccttgcg gtattcgtac ccctgattct gcaaatcggc gattaactcc
    23401 gcttctaacc ggttttccga ttggtagctg cccgactgtt cgattttttc atattggtcg
    23461 agcacgatga aattcggcgt ttcggcgatg ggtttggttt cgaggttcat gggtcgtcct
    23521 ttgcggtgtc gggttttata gtgaattaaa tttaaaccgg tactgcgttg gctcgcctta
    23581 gctcaaagag aacgattccc taaggtgctg aagcaccagg cgaatcggtt ccgtactatt
    23641 tgtactgtct gcggctcgcc gccttgtcct gatttttgtt aattcactat aaacggtttg
    23701 atgttcggat tgggctgcct gaaaaagtgc aggctgcttt ttcaggcagc cttggggaag
    23761 gcaagcagct gttcgcggta atattcgtat tgtttgcggc gcagggcgat ttcgtgcggc
    23821 aggccttcgc tgacggagtg ggtcagggta tcgaatttgc ctaggatggc gacgattttt
    23881 tcctgttcgg ggagcggggg gatggggatt ttatattgca taatcgctgc tttgctgcca
    23941 cgcggcattt ttgcaccttt ggcgtgcttc atattaaaag caaaaaattt atcatcggcc
    24001 aatacctgat acagatattt tggattaacc ttttcatctg ttacccggat aaccaaaaca
    24061 tcaccgtttg ttccgcccgt gcagtctgcc tgccagattt ttttcaaata cggacggata
    24121 tttccaatca aaatgtcatt gacaatatat tctgtcattt ttccttcgct tggaacataa
    24181 ccggataact ttttaccttc tctgttttgt aagagattat ccacgccaac gtaattatgt
    24241 tcgttcagtt tatccgaaca aatacggttt tttgaatatt cggcaacctc ccccaacgtc
    24301 ttccaaacca catttttcag acggcattga tagccgtcag ctatcccccc cccgatttga
    24361 ttgtcaaaat ctaaaagtaa gtcgcggtaa taccggtatt ggcgtttgcg cagggctaat
    24421 tccgcttcca gcgtagcttc cagctcggtg aatttgtcaa gtatttttac aattttttgt
    24481 tgggtttcca atgatgggat ggggatttta tagttatctg tatctggtgt tgctatttgt
    24541 ggcatttgca tttttgatcc gatattacga aaatagtttt cttgtgtttt taagaaataa
    24601 taaacatatt ttatataaat atgtttatta actgaatgat aagaccacat ttcattttta
    24661 tgcgaaaatg gtttatcgta atattcgaat tcaataatac ctcttgattt aacgataata
    24721 gaaggctctc tatggatatc cttatcagga acatcatcaa agtcaactaa tgcaaaagtt
    24781 tttcctcctg caaaaatctt taacggcgca ttatctttat gcatttcttt catttgtcca
    24841 gcagttatct tagttccttt tgtacgaact aaaacctccc ccaatggctt ccactccacc
    24901 ggtgccgtct gaatcatctc aatcaatttt ttcgctttgc tttgcatatc catggttgtt
    24961 ttccgtttcc ttcccgctgc ggcgggatat ggggcggttg cggggcgggt ttcggctcac
    25021 tctgtccgtt tgccgttgtt tctgcgggct gaaacccgtc ctgctttcag gctgcctgaa
    25081 atcgtgtgca ggctgctttt tcaggtagcc tgaatgcggt tattttttgc cgttttgatg
    25141 ctgttcggtt gcggcaagcc ttttctgttc ttcgttctca agctgtttga tgcttttctc
    25201 gggtacgggc tggttttcag gcatgatgcc gcccagttct tcaatggttt ggcgcacttt
    25261 ttgtccgact tcgaaatgca cccggtttgc ctgtgttttg ccttggatgt ttttgcggcg
    25321 tagtttttct tcggtttgcg tggcgcgaaa cagattggca gccggttcgc tggcgttcat
    25381 gtggtctagg attctctggc ttttcttcaa gcctttgcgg ctgtgtatgc cctgcttgtc
    25441 cagtcctccg tatagccccc gatagccgtg gttttggaag acggcgtact caacgggttt
    25501 ttctacgcct gcatccttgg ctgcggcggc aaggtcggta ttgtgttcgc gcagctgcct
    25561 gcgaagcagc aggcgttgtt tgtcttcgcc caaactcctg aatgctgcct cgtcttgcag
    25621 ctcttggcgg cgggcttgca cggcaaagta ggtttgccct gcggcaatga cgcttttgga
    25681 cggatcgccg ttttgcacaa tcaggtagca ggcatagcgg gaaaggcggt aatccgccac
    25741 ttcgcgctgc ccgcctttcc caagggctat cattttgttg gtttcaacaa aatgatgatt
    25801 tttgtcattt cctgatgttt cgcaagcggt tatggcttta tcaatggccc gttggaaatt
    25861 gcgccattcg gaatattcca agatttgctg caaggtgcgc gccgaccaat attcattgcc
    25921 tgtttcgtcg atgtgtttgg cgttttcaaa agcgttattt tctgttgtca tgtattttcc
    25981 ccgaaaattt cttgatttga acttgtaagg attgcttaca ggttgccgcc cgtattttca
    26041 gacggcctct ttcccattca gttgttgcaa aatatgcaac aactgcctag gtttcaatct
    26101 ctgcaatcac ttcgtcaatt tcacgccgca gccgttcgat tttggcgacg gtttcgctga
    26161 tttcggcgtt gagctgtctg atgtcgatga cctcgcgggt gtcttcggct tcgacatagc
    26221 tgctgacggc gaggttgtag ccgttgtctt tgacggtttg ctgggcggcg ttttgggcga
    26281 tatgcggcac atcggctttg tcggcgaaga gtttgacgat ttcggcaatg tgttcttcgg
    26341 ttaagacgtt gttgttggtt tcttttttaa agaagccgcc tgcatcgatg aattggatgt
    26401 cggtattgtc tttgtgtttg gacaaaacca ggatattgac ggcgatgcag gtgccgtaaa
    26461 agagattggg cgcaagggca atcacggttt ccacatagtt gccctccacc agatattggc
    26521 ggattttctg ctctgcgccg ccgcgataga aaatgccggg gaatgagacg atagcggcgc
    26581 ggcctctgcc ggaaaggtag ttcagtgcgt gcaggatgaa ggcaaaatcg gctttggatt
    26641 tcggtgcgag tacgcctgcg ggggcaaagc ggtcgtcgtt gatcaaggtg gggtcgtcgc
    26701 tgcctatcca gtcgatggaa tagggcggat tggagacgac ggcatcaaag ggtttgctgt
    26761 ctttgagttt ggggttggtc agcgtgtcgc ccaattcgat gtggaatttg ttgtaattga
    26821 cgttgtgcag aaacatattc atgcgggcga ggttgtaggt ggtgtggttg atttcctgcc
    26881 cgaagaagcc ttcttcgatg atgtgttcgt caaactgttt tttcgcctgc aagagcaggc
    26941 tgcccgagcc gcaggcgggg tcgtagattt tgttgacttt ctcctgcccg tgcaccgcca
    27001 gccgcgcaat cagcttggag acgctttgcg gggtgaaaaa ttcgccgccg gatttgcctg
    27061 cgttggcggc gtagttggaa atcaggtatt cgtaggcatc accgaaaagg tcgatgcggt
    27121 ggtcttcaaa attgccgaaa tcgagttccg ccacgccttt aaggacggcg gcaaggcgtt
    27181 tgtttttgtc ggcaacggtg ctgccgagcc ggctgctggt ggtgtcgaag tcgtcaaaca
    27241 agcctttgat gccttgttcg gacgggtagc cggaggcgga gctttcaatc gcggtaaaga
    27301 tttctttcag cttggtgttg agctcttcgt tttgatgggc ttcggcggca atattgcaaa
    27361 aaagctggcc ggggtagatg aaatagcctt tgactttgac ggcatcgtct ttgatttcgg
    27421 gcgtgatgat gctgtccggc atagcggcgt aatcaatgct gctgtcgccg gcctgcatat
    27481 agtcggtgaa gttttcgctg ataaagcggt agaaaagtgt gccgagaacg tattgtttaa
    27541 agtcccagcc atccaccgcg ccgcgtactt cgtcggcgat tttccaaatt tggcggtgca
    27601 gttgggcgcg ttgttgcatt tcggtcatca tcgaaatcca tatataaagt taaacaaatc
    27661 aaaatcgcct gatattttca gacgattttt ttacgggcat tcaaattaag ccaaattttc
    27721 cagcatccat ttgacgtagc ggttgacacc tgctttgaca tcgaaaaatt cttccttata
    27781 tccggcttcg cgcaatttgg tgatgtcggc ttgggtgaag ccctggtatt tgcctttgag
    27841 cgcgtcggga aaggggatat agcggataag ttcttcttct accaactctt tcaagctcaa
    27901 ttcagatttg ccttcggcgg cgcggcaggc gttgacggcg gcggcggcga gttcgttgaa
    27961 ctgttggctg cggccggtgc cgaggttgta gatgccggaa agttcgggat ggtcgaagaa
    28021 gtagaggttg attttggcga cgtcttcgac gctgacgaag tcgcgggttt gttcgccgtt
    28081 gccgtagccg tcgttgctgc cgaacaggtt gacgtaaccg tgttcgcggt attggtggaa
    28141 atggtggaag gcgacggatg ccatgcggcc tttgtgttgt tcgtgttgtc cgtaaacgtt
    28201 gaagtagcgg aagccgacga cttgggcggt gagaccttct ttcatgcggc ggcgcaatac
    28261 ttggtcaaac aggaatttgg agtagccgta cacattaagc ggtttttcga gttcgcgctc
    28321 ttcgcggaag atttcgcctt tgccgtaaac cgccgcactg gaggcataaa ggaaggggat
    28381 gcgttcgtcc tgacaccagt ccaacaaatc cagcgtgtac tggtagttgt tttccatcat
    28441 atacaaaccg tcgtggttca tcgtatcgga acacgcgcct tgatggaaaa cggcttcgat
    28501 gttttgataa ggtaaaatgt gttccctcac ttggcggata aattcgtgtt tgtcgaggta
    28561 gtgggcgatt tcgcactcgg caaggttttt gaatttttcg ccttttgtca gattatcgac
    28621 ggcgacgatg tcggtaatgc cgcgttggtt gagggctttg acgatgttgc tgccgataaa
    28681 gccggccgcg cctgttacga tgatggtcat attcggtttc ctttgttttg agggtttcag
    28741 acggcattgg atggtcatgc cgtctgaaaa gatttattgc ccgtccagtg cttcaaccaa
    28801 ctctgcaaac gagcaaaccg ccgtaccgag tttcgccacg acaaccccgg ccgcagtatt
    28861 ggcaaggtac atggcttcgg gcatggtgca gcctgccgcc agccccaagc ccattccggc
    28921 aatgacggtg tcgcctgcgc cggacacgtc gtaaacttct tgggcgcggg tgggctggta
    28981 aatgggttcg ccttcgctga acagggtcat gccctcttcg ctccgggtca gcaaaacggc
    29041 ggtcaagtcg aggtggcggc gcaggttttg cgctttttcg gtcagatcgc cttcgttttt
    29101 ccaactgccg accacttctt tcaattcggc gcagttgggc gtaatcagag ttgcgccgac
    29161 atatttttcg taatcgtcgc ctttggggtc gattaagacg gttttgccga catgtttcgc
    29221 ccaatcgatc atatcggaga tatgcgacag accgcctttg ccgtagtccg aaaaaatgat
    29281 tacgtcgtat tcgggcaaga tttcacggta tttccgcttg atttgttcca acacttcgcg
    29341 gttgggatgt tcttcaaaat caaggcggat aagctgctgg ttgcgggcga cgacgcgcag
    29401 tttgacggtg gtggcgattt gtttgtcgcg catcagatag gaggcgacgc cgtcctgcac
    29461 catcagcgcg tcgagcgcgg cggcggcttc gtcgtcgccg gttacggaca acagccctgc
    29521 cctgccgccc aacgaagcga tgttgcgcgc aacgttcgcc gcgccgcccg cccgttggtc
    29581 gattcgtccg atttttgcca ccggcacggg tgcttcgggc gaaattcggg acacgtcgcc
    29641 aaaccaatag cggtcgagca tcacgtcgcc gacaacaaga actttggctt gtgcgaaacg
    29701 ggatttgagg gtttcttgtt ggaacttggc gggcatattg cctccgttgt gttttgttca
    29761 gacaacctta acctgtaaga ccgcctgaaa atcagtttgc ttcaaggttt gccacgcggt
    29821 tcacttcgcg caatacggcg accggatcgg cggctcgggt aacggggcgg cccatcacca
    29881 gataagtcga acccgccgcc aaggcttcgg cgggtgtcat aatgcggcgt tggtcgtcgt
    29941 tgttgccggc aacgtccaag cggatacccg gtgtaaccaa gacaaaatcc cgtcccaatt
    30001 cgcggcgcag cggcgcggct tcttgggcgg aacagaccac gccgtccaag cccgaacttt
    30061 gtgccagttt tgccaaacgg atgacttgtt cttcaggggc ggtgttcaaa ccgatttccg
    30121 ccaaatcgct ttgctccatg ctggtcaaca cggttacgcc gattaagagc ggtttcgtgc
    30181 cgtatccggc aacggcttcg gcggcggctt ccatcatacg tctgccgccc gacgcgtgca
    30241 tatcgaccat ccacacgccc atatcggcgg cgactttaca cgcctgcgcg acggtgtggg
    30301 gaatatcgtg gtatttcaaa tcaagaaaga gtttgaaacc ctgatgaatc aggctttccg
    30361 ccaaactgcg ccccgtagcg gtaaacagct ctttgccgat tttgatttgg cacaacgccg
    30421 gatcaaggtt gcggacgaat ccgagcgtgt ctttttcgtt ggcaaaatca agggcaacga
    30481 tgacgggggt acgttgttgc ggagtttgga agtcggtgat taaaggattc atggtgtttt
    30541 cgtttcgggt tttcagacga cctttatcgt ctttttcgca gtatagcgga ttaacaaaaa
    30601 ccggtacgtc gttgccccgc cccggctcaa agggaacgat tccctaaggt gcccaagcac
    30661 caggcgaacc ggttccgtac tatccgtact gtctgcggcc cgccgccttg tcctgatttt
    30721 tgttaatccg ctataacaac gtaaattcaa accgtaggcc gggcattgat gtccgaccta
    30781 cgcggactca agcccccgac aaactgtttc ggataaattt gaaccggtac atcagccgtc
    30841 attcccgcgc gggcgggaat ccattgataa aactcagttt atcagattta aacagcgatt
    30901 gcccaaattc agaaatggat tcccgcctgc gcgggaatga cgccgaatga ttatttttcg
    30961 gttgcaacac gctgtttatc aaaaacaggt cgtctgaaaa cctgattttc tgttttcaga
    31021 gtgcgaagcc tgcccgtggg tacgaccttt tcgatttatt tgcccaatgc gcccaatact
    31081 tcggctttca cagcttctac tgcttgggta ccgtcgactt tgatgtattt cggcgcgtgt
    31141 tcgccttcca gcttgctgta aaaatcaacc aaaacttcgg tttgctcgtg gtaaacggca
    31201 agacgttttt tcacggtttc ttctttgtcg tcgtcgcgct gaatcaaatc ttcgccggtt
    31261 acgtcgtctt tgccttcggt tttaggcgga ttgtaggtaa cgtggtaagt acggccggaa
    31321 gccaaatgca cgcggcggcc gctcatgcgg tcgacaatca cgctgtcgga tacgtcgatt
    31381 tcaacgaccg cgtccaaacc cacgcctgct tccaccatcg cttcggcttg cgccaatgta
    31441 cgcgggaaac cgtcgaacag gaaaccgttt ttgcagtcgt cttgcgcgat gcgttctttg
    31501 accatgccga taatgatgtc gtcgcgcacc aaaccgcctt catcgatgat ttttttcgct
    31561 tccaaaccca agggcgtgcc tgccttaatc gcggcacgga gcatgtcgcc ggtagagatt
    31621 tgcggaatgc cgaacgcggc ggtgatgaat tgcgcctgag tgcctttgcc cgcgcccggt
    31681 gcgcctaaaa gtaatgcttt catgaagtga tccttttaaa tgtgtttatt ggaatcaaac
    31741 agatggttta cgattctaca cggcttggcg caatagttca aaaaccaact gcacaaactg
    31801 ccatttgcaa gcaaatgttt tgcatacctt tgccgagcct gatattcaca aggcggcaac
    31861 tttttgggac aatccccaaa gtttcaaaac atcacaccct accaaaaagg aacatccgtg
    31921 aaacgcattt ttctgttttt ggctaccaat atcgctgttt tggtcgtaat caacattgtt
    31981 ttggcggttc tgggcatcaa cagccggggc ggcgcgggca gcctgttggc gtattccgcc
    32041 gtcgtcggct tcactggttc gattatttcg ctgctgatgt ccaaatttat cgccaaacaa
    32101 tcggtcggtg cggaagtcat cgacacgccg cgcaccgaag aagaagcctg gcttctgaac
    32161 actgtcgaag cccaagcgcg gcaatggaac ctgaaaacgc cagaagtcgc catctaccac
    32221 tcccccgaac ccaatgcctt tgccacgggc gcatcgagaa acagctccct gatcgccgtc
    32281 agcaccggtt tgctcgacca tatgacgcgc gacgaagtgg aagccgtgtt ggcgcacgaa
    32341 atggcgcacg tcggcaacgg cgacatggtt acgctgacgc tgattcaagg cgtggtcaat
    32401 acctttgtcg tgttcctgtc gcgcattatt gccaacctga ttgcccgaaa caacgacggc
    32461 agccagtccc agggaactta tttcctagtc agcatggtat tccaaatcct gttcggcttc
    32521 cttgccagcc tgattgtcat gtggttcagc cgccaacgcg aataccgcgc cgacgcgggc
    32581 gcggcaaaac tggtcggcgc accgaaaatg atttccgccc tgcaaaggct taaaggcaac
    32641 ccggtcgatt tgcccgaaga aatgaacgca atgggcatcg ccggagatac gcgcgactcc
    32701 ctgctcagca cccacccttc gctggacaac cgaatcgccc gcctcaaatc gctttaaacc
    32761 gatttgaaac gacaaaaaac cgtacgcaca agcagtacgg ttttttcgta tatgccgact
    32821 gaaaacatca ggtttccata ccggcaccaa ccgtatccat ttccgcaccg gatacgccgc
    32881 attgatatat ccccgacaaa ccgtgtccgg acagaaaata tcagataaaa cagcggtaaa
    32941 aatgccgtct gaaaccaatt agggcttcag acggcatttt tactgcaaaa ccttatgcga
    33001 acggatgatg cagcacaatc gtttcttcgc ggtcggggcc ggtggagacg atggcgaccg
    33061 gcgcgccgca gacttcttca atccgtttca aatatgcttt ggcattggca ggcaatgcgt
    33121 cgtagctttt cacgccgaca gtcgattcgc gccagccggg catggtttcg taaatcggtt
    33181 tgcaggtttc caccgcatcc gaaccgcaag gcaggatgtc ggttttgccg ccgcctggca
    33241 attcgtagcc gacgcagata ttgatggttt caacgccgtc cattacatcg agtttggtaa
    33301 tgcacatacc ggaaatgccg ttgatttgga tggagcgttt cagggcggcg gcatcaaacc
    33361 agccgcagcg gcgcgcgcgg ccggttaccg aaccgaattc gtgtccgcgc tccgccaaac
    33421 ctgcgcctac ttcgtcgaac aattcggtcg ggaacgggcc cgaaccgacg cgcgtggtat
    33481 aggctttgac gatgcccaaa acataatcca gcatttgagg acctacgccc gcgcctgccg
    33541 aagccgcgcc ggcgagacag ttggacgagg taacgaaggg gtaagtgccg tagtcgatgt
    33601 ccaacaacgc accttgcgcg ccttcaaaca gcagtttttc gccgtttttg tttttttcgt
    33661 tcaacacgcg ggacacgtcg gcaatcatcg gcgcaatgcg cggcgcgact ttttcgataa
    33721 ccgccatcac gtcttccgct ttaaccggtc cggcgttgtg caggtattgg agttggacgt
    33781 tgtaataggc aaggacggca tccagttttt cacgcagttt ttcaggatgc agcaaatcgg
    33841 cggcgcgaat ggcgcggcgt gccactttgt cttcgtaggc agggccgatg ccgcagccgg
    33901 tcgtgccgat tttgcctttg ccgcgcgatg cttcgcgggc ttggtcgagc gcgatgtggt
    33961 aaggcaggat cagcgggcag gtcggcgcga ttttcagacg gccttcgacg tttttcacgc
    34021 ctgccgcgtt caactcgtca atttcgccca acagggcttc gggggaaacg acaacgcccg
    34081 aaccgatgaa gcaatccaat ccttcgtgca ggataccgct cggaatcagg cgcaaaatgg
    34141 tttttttgcc gccgacgacc aaggtatggc ccgcattgtg gccgccttgg aagcgcacga
    34201 cgccgccggc ttcttccgcc agccagtcaa cgattttacc tttaccctcg tcgccccact
    34261 gtgcgccgat tactacaaca tttttagcca tagccatata acctatcgat attaaaaatt
    34321 attgcggaaa atccccaaac cggtgcggct ttgcgccccg ccccggtttg cctttcatcc
    34381 gctatgaacg ggtatttagc gtttcacgac ctgccaaacg ccgtccgtct ttttcagacg
    34441 gcctgcaagc tcttccgaaa cattgtgtcc gataccgtag tcaatcacga cacactgccc
    34501 ttgttcacgc aaggcttcga ccgcttcgcg cgccgcttct gcatcttccg catcgaccga
    34561 cacggcgggc tgccgttcga cggcgggcaa acgcccgata aagctgcgta agtcgaaact
    34621 gaatcccgtt gccgggcgcg ccctgccgaa atatccgccc aatccgtcat aacgcccgcc
    34681 gcgcgcgacc gcgtcgtgga aatcggcggc ataggcggca tacagcaagc ccgtgtggta
    34741 attgtcgacg cgcagctcgg acaagtcgat atggatttca ttatcgggga atgcgtcgca
    34801 caccgcctgc aattcgtcca acgcgccgcc gaccgccgac aaatccggca accgtccgcg
    34861 cgcgtcggac aacacttcac gcccgccgta caggcgcggc agcaacgaga atgcttttgc
    34921 ccacataccg tccagcttcc aagccttgac ctgcgcttcg accgacccgg tatctttatc
    34981 ctgcatcaag gcaagcagcg ctgcggactg ccccgcatcc aaatgcgccg catcggacaa
    35041 ggcgcggaat atgccgatat gccccagcga aagcagcact ttgcccatat cggcaatttt
    35101 catgcttttc agcataaggt ctatcagctc gatgtcgcca cggatgtcgg caaaaccgta
    35161 catttccgcc cctgcctgca agggttcgcg catattcggc agaccgtcgg gctgcgcgtg
    35221 caacaccgga ccggcataac acaaccggtt aatcccttgg ttggcggaca aaagatgggc
    35281 atcgatacgc gccacctgcg gcgtgatgtc ggcgcgtatg cccaactgcc tgccgctgag
    35341 cctgtccgtt accaaaatgg ttttcagtga aagccccgca tcgatatgcg tcagcaggga
    35401 atgtgcgtac tccatcagcg gaggctgtac cagttcataa ccgtgtacgc ggaacagtgc
    35461 caacaactgc tcgcgcgcgc tttcaagctg ccgcgcgttc gtgggcagta cgtcggcgat
    35521 atgttccgga agctgccatg tctgcatgag aatctgcctt ctgttttatc ctgtaaacac
    35581 aaaagactgc ccgatccgca atcagacggc atctttgccg caacgcatca cgccgctgtc
    35641 aaaacggcga tgcttccttc cgattcagaa tccgaacagc ccctgtccga cggctcaatg
    35701 ccgtcaagtc gttaaaacca aactttacca taaaatacac acaatctgaa tatactttat
    35761 ataccccgta tccgccgtct gacggcacgg tattttttta ctatataatg tgcaccatta
    35821 tttctcatca cttcccgctg ccgttttttt gtttcagacg gcatttttat tgactgaaca
    35881 cttatgctgc tcgacctcga ccgcttttcc ttttccgtct tcctgaaaga aatccgcctg
    35941 ctgaccgccc ttgccctgcc catgctgttg gcgcaggtcg cgcaggtggg catcggtttc
    36001 gtcgataccg tgatggcggg cggtgcgggc aaggaagatt tggcggcggt ggctttgggc
    36061 agcagcgcgt ttgccacggt ttatattacc tttatgggca ttatggcggc gctgaacccg
    36121 atgattgccc agctttacgg cgcgggtaaa accggtgaag caggcgaaac ggggcggcag
    36181 gggatttggt tcgggctgat tttggggatt ttcggcatga ttttgatgtg ggcggcgatt
    36241 acgccgttcc gcaactggct gactttgagc gattatgtgg aaggcacaat ggcgcagtat
    36301 atgctgttca ccagcttggc gatgccggcg gcaatggtac accgcgcact gcacgcctac
    36361 gcttccagcc tgaaccgccc gcgcctgatt atgttggtca gctttgcggc gtttgtgttg
    36421 aacgtgccgc tgaactatat tttcgtttac ggcaaattcg gtatgcccgc tttgggtggc
    36481 gcaggttgcg gcgtggcgac aatggcggtg ttttggttca gcgcgctggc attgtggatt
    36541 tatatcgcca aggaaaaatt cttccgcccg ttcggactga cagcgaaatt cggcaaaccg
    36601 gattgggcgg tgttcaaaca gatttggaaa atcggcgcgc ccatcgggct gtcttatttt
    36661 ttggaagcca gcgcgttttc gtttatcgtg tttttgattg cgcctttcgg cgaggattat
    36721 gtggcggcgc agcaggtcgg catcagtttg tcggggattc tctatatgat tccgcaaagc
    36781 gtcggctcgg cagggacggt gcgcatcggc ttttcgcttg ggcggcgcga attttcgcgg
    36841 gcgcgttata tttcaggagt gtcgctggtg tcgggctggg tgctcgccgt gattaccgtg
    36901 ctttccttgg tattattccg ttcgccgctg gcaagcatgt acaacgatga tccggcagtt
    36961 ttaagcatcg cctccaccgt cctgctgttc gccggcctgt tccaaccggc agacttcacc
    37021 caatgtatcg cgtcctatgc cctgcgcggc tacaaagtca ccaaggtgcc gatgttcatc
    37081 cacgccgccg ccttctgggg ctgcggcctg ctgccgggct atctgctcgc ctaccgtttc
    37141 gatatgggca tttacggctt ctggacggca ttgattgcct cgctcaccat cgcagccgtc
    37201 gccttggtgt ggtgcttgga aaaatacagt atggagttgg tcaaatcaca caaggccgtc
    37261 tgaaaacgca gcacacttca attcaaatac agttaaagct caaaccatgc aacccatccg
    37321 ataccgaacc gacctcaccc cctacaacac tttcggcctg cacgcccaag cccgagcctt
    37381 tatcgcgctt aaacacgccg acgaattgcg cgacatcgtc cgactgcccg agttcgaccg
    37441 cgatactgtt ttatggctgg gcggcggcag caacatcctt ttgatgcagg attacgacgg
    37501 cttggtcgtc catatggaaa acaagggcat acgcgagatt gcgcgctcag acggcatggt
    37561 tctgattgaa gcgcaggcgg gcgaaatttg gcacgatttc gtgttgcaca ccgtcgcgct
    37621 gggtttgagc ggtttggaaa acctgagcct gattccgggt acggtcggcg cgtcgcccgt
    37681 gcagaacatc ggcgcatacg gcgtggaggc gaaagatgtg attcacagcg tgcgctgctt
    37741 cgatttggat acggaaacct ttgtaaccct ttccaatgcc gactgccgct tcgcctaccg
    37801 cgaaagcctg ttcaagcagg aaggcaaagg gcgttatgtg attgtttcgg tcgtatttgc
    37861 attaaaaacg cattttgtgc cgaacttggg ctacggcgat ttggcggcga aggttgccga
    37921 attgagtgca ggtagggagg cgacggcgaa agacgtttcc gatgcggtat ccgccatccg
    37981 caacagcaaa ttacccgacc cgaaagtttt gggcaatgtc ggcagtttct ttaaaaaccc
    38041 cgtcgtcagc acggaaaaag ccgccaccct gttgcagcgg catcccgata tgccgctcta
    38101 tccgcagccc gacggttcgg tcaaactcgc cgccggctgg ctgatcgacc aatgccgtct
    38161 gaagggcttc caaatcggcg gtgcggcggt acacgataaa caggcattgg ttttggtaaa
    38221 caaaaacaac gcctcggcaa acgatgtccg gcagttggcg caacacatca aatttacagt
    38281 atttgctcgg tttcaggtag aattacacgc cgaacccaat tggctgccca cttcgttcag
    38341 cctgtaaatc caaggagtat gcccgtgacc cgcattgcaa aaaccaatac ttacacccgc
    38401 atcatcgacg ccagccttgc gcttttcaac gaggaaggcg agcgcaacat cagcaccaac
    38461 catatcgcgg cgcatttggg catcagtccg ggcaatcttt attaccactt ccgcaacaaa
    38521 gacgaaatca tcgtccaact cttcaaacgt tacagcgaag ccctgttggc atacctgaat
    38581 gaagccgtgt tgccctctga tgtggaagac tctatcaact atatggcagg catttacgat
    38641 gtcatgtggg aataccgctt cctctttagc gacgtaaaca ccctacttgc gcgcagtgcc
    38701 gaattgttgg gcgaacacaa tacctttacc caagccaaag tctcgccgct tctagtcaaa
    38761 ctgctgaccc agctcaacgg gctgaacgtc atcaacgccg accaaaccgc tatgaacgac
    38821 ctcgccgtca atatgtggat ggttacgaaa tactggttcg acttcgacag ttccctgcgc
    38881 ggccgtacca agctgaccga agactccaaa gcacgcggcg tccgtcgtac cttaagcctc
    38941 cttcgcccct atcttttgcc cgaacaccgc gcggaatacg accgcaaaat cagcaacggc
    39001 aacccgtaaa cccataacgt ccgaatcagc cttcagacgg cacttacccg acggtatcag
    39061 cgaatgcgcg cagtcccccc gccccatatc tccatcacag gcctaggtta cctcggcctg
    39121 ccgctggcac aaaagtttta ccgacacggc agccgcgtcg ccgccataaa acgcagcctg
    39181 acttcggacg acatcaacct gcccatacac ctcgacacct tcgacctcaa ccgtaccgat
    39241 acctttcaga cggcattgtg gcggcatcac gccgacaaac ccgtctggtt cttcctgctg
    39301 ccgccctcct ttttggcgca ttacgccgac accgtcaaac aatgggcaga gcttgcccgg
    39361 gcgtgcaacg tgcaacacct gattttcaca agcagcacaa gcgtttacgg cgataaagcg
    39421 cgagaatgca acgaaaccgc cgcacccgat ccgcaaaccg agtccgcccg acaaatcctt
    39481 gccgccgaac aacacctgct cgacaacggc gttccgaaca tcgacatcct gcggctgggc
    39541 gggctttatt gcgccgaacg ccatcccgtc agccgcctcg tgcaaaagca aaacatccaa
    39601 ggcggcaacc ggcctgtcaa tatcgtccac cgcgacatcg ccgtcgaaaa cctgtttcag
    39661 acggcatcca accccggcgg caggcggctg aaaaacatta tcgaaccgcg ccacccgaca
    39721 cgccgcgaat tttatacgga agaagccgcc aaactcggcc tgcccgcgcc ggatttctca
    39781 cccgacgaca gcgtgggcaa aatcatccgt accgtttgcg ataacggctt aagcctgtaa
    39841 aataagcggc aacagcaaca acaggcattc tccgccccaa tcagaaaata tgtccgccct
    39901 cctccccatc atcaaccgcc tgattctgca aagcccggac agccgctcgg aacttacctc
    39961 ctttgcaggc aaaacactga ccctgaacat tgccgggctg aaactggcgg gacgcatcac
    40021 agaagacggt ttgctctcgg cgggaaacgg ctttgcagac accgaaatta ccttccgcaa
    40081 cagcgcgata cagaaaatcc tccaaggcgg cgaacccggg gctggcgaca tcaggctcga
    40141 aggcgacctc atcctcggca tcgcggtact gtccctgctc ggcagcctgc gttcccgcgc
    40201 atcggacgaa ttggcacgga ttttcggcac gcaggcaggc atcggcagcc gtgccaccga
    40261 catcggacac ggcatcaaac aaatcggcag gaacatcgcc gaacaaatcg gcggattttc
    40321 ccgcgaaccc gagtccgcaa acaccggcaa cgaagccctt gccgactgcc tcgacgaaat
    40381 aagcagactg cgcgacggcg tggaacgcct caacgaacgc ctcgacaggc tcgaacgcga
    40441 catttggata gactaacctt cagacggcat ccgacatgaa cagccctttt cacaacatcg
    40501 gtatcgtaac ccgccccaac acgcccgaca tccaagatac cgcacacacg ctgattacct
    40561 ttttgaagca gcacggcttt accgtctatc tcgacgaagt cggcgtaagg gaatgctgca
    40621 tctataccca agacaccgac ggctgccata tcgtcaacaa gaccgaactg gggcaatact
    40681 gcgacctggt cgccgtctta ggcggagacg gcacctttct ctctgccgcc cgcgaaatca
    40741 ccccccgcgc cgttccgatt atcggcatca accaaggtca tttgggcttt cttacccaaa
    40801 ttccccgcga atatatgacg gacaagctat tgcccgtttt agaaggaaaa taccttgccg
    40861 aagagcgcat cctgattgaa gccgcactga tccgcgaagg caaaaccgcc gaacgcgccc
    40921 tcgccctcaa cgatgccgtc ctctcccgcg gcggtgccgg acagatgatt gaatttgaag
    40981 tcttcgtcaa tcaggaattc gtctatacac aacgttcgga cgggctgatt gtctccaccc
    41041 ccaccggatc gaccgcctat tcgcttgccg ccggcggccc aatcatgcag gcaggattac
    41101 acgccttcac gctcgtcccc atctgcccgc aatctatgac taaccgcccc atcgccattc
    41161 cagacacgtc cgaaatcgaa atcctcgtta cccaaggcgg cgacgcgcgc gttcatttcg
    41221 acggacaatc cttcatcgac gtgcaaaacc tcgaccgcat catcatccgc cgctaccaca
    41281 atcccttacg catcctacac ccgaccgact accaatattt caaaaccctg cgccaaaaac
    41341 tgcactgggg cgagcaatta gtctaagccg gccttcaccc gaaagtaccc atgaattcca
    41401 aaatttcctc cgaccataca gaagatacca tacgcctctc caaacgtatg gcacaactgg
    41461 ggctttgttc gcgccgcgaa gccgacggcc atatcgaaca gggctgggta acggtcaacg
    41521 gcaaaaccgc cgtactcggt cagaaagttt caccggcaga ccgtatcgaa ctgaacaaga
    41581 aagcccacga acagcaggcg gcacgcatta ccatcctgct gaacaaaccc gtcggctatg
    41641 tcagcgcaca agcggaaaaa ggctataaat ccgccgccga actgattacc cctgaaaatt
    41701 gctgggaagg cgacaccagc cgcatcagtt tcgatccgaa acacaaaatc ggtcttgccc
    41761 ccgccggcag gctcgatatc gattcagtcg gattgctggt gttgacgcaa gacggccgta
    41821 tcgccaagca gcttatcggc gaaaacagcg gcagtgaaaa agaatatttg gtgcgcgtgc
    41881 gcggcaaatt ggacgaaaaa ggacttgcct tactgaatca cggattgagt ttggacggtg
    41941 ataaactgcg tcccgccaaa gtagaatggc aaaatgaaga ccaactgcgc ttcgtgttga
    42001 aacagggtaa aaagcggcaa atccgccgta tgtgcgaact ggtcggactg cgcgtcgtcg
    42061 ggctgaaacg catccgcatg ggcaaggtca aactcggcag gctgccgccc ggcaaatggc
    42121 gttatctcgc tcccggcgaa tcgttttaaa taagcattaa aaaatgccgt ctgaaaatcc
    42181 ttgcgttttc agacggcact gccttatgcc caaataacgg tttcttccaa gggcctgcgc
    42241 gctttcgtga cgatttcctg aacccggtag ccgaatgtcg cggcaacgga cacgccccat
    42301 tctgccgcat cgaacaaacc gaactgcccg gacaatacgc gttccatatc ggcatagttg
    42361 aaaccttcca ccgggcagga atcgataccc gccatcgccg cacccgtcat catgttggct
    42421 aacgcgatat aggtctggcg gcagcaccag tcaaacaagg cgcgcgaatc gtccaaaatc
    42481 ttgatgtcgt cagcttgaaa cgcctgatac ctcgccaaag atttttctac ggcatccggt
    42541 tcggtaacgc cgcgccgttt gagactttcc aacataaacg ggctgtcgaa gcgggcattt
    42601 ttcttcgcca aaaacaccac caaatgactg gcggtatcca aagcatccgc cataccccaa
    42661 gaaaacagct tgattgcctg tcggatttcg gggttttgaa ccacaacaaa ctgccaaagc
    42721 tccgaaccga ccgaactggg cgacaaacgc ccgagttcta aaataaactg aaaatcctcg
    42781 gcactgattt tacgtgccgc atcgtaatgc cggcacgatt tgcggttttt aaatgcggat
    42841 agaacctgct ctttgcttaa tactgtcatc gtgcatcctt ttattacttt gattgcaaat
    42901 tgatttcaaa ccgatacggc actaccgcac cctataagcc gtccccgcca tcaagcttca
    42961 cccggatttt acccatctct ctacggctgg gaaaacaaag cggcggcaga ttcgggaata
    43021 actccccaat gcctttcaga cggcatttgg acatcgcaaa cgcgcctacg ggtaggcgat
    43081 ataggcataa gccgaatggt tgtggataga ctcgaaattc tcgctctcga cgacgaaact
    43141 cttgatgcgt ttgtcggcaa tcagcgaagt agcgacatcg cgcaccatat cttccacgaa
    43201 tttcgggttt tcgtaggcct tttcggtaac gtatttttca tcggggcgtt tgagcaggcc
    43261 gtagagttgg cagctcgcct gcgtttccac ataatcgata acttcctcga taccgacttc
    43321 ggcatcggaa gtcaggctga cggtaacgtg cgaacgctga ttatgcgcgc catattggga
    43381 aatttctttg gaacacgggc aaagcgaggt tacgggaatc atgaccttca tactgtggcc
    43441 gtatgccccg tctttcattt cacccgtgag gctgacatca taatccagta aggaccggat
    43501 accggatacc ggcgcggttt tcttgcggaa aaacggaaaa gaaacgctga ttttgccggc
    43561 gcgggaatcc aaaagcgcga ccatttcggc agtcagccta tgcaattgtg cgaaatccaa
    43621 gacttcggta tgttgctcca tcaatgcgac aaagcgcgac atatgcgtcc ctttctgctc
    43681 ggcgggcaga taaaccgtca tcgtcaggcg agcaaccgtg gattgtgttc cttcggcggt
    43741 tttcagggta atcgggaagc gcaggtcttt aatgccgacc tgattgatcg gcaggttgcg
    43801 caaatctctg ctggattgca cgtctgcaat ggcgttcatg cgttgtttgt ccttaatatt
    43861 cagataatta ttgagatgtg tgtattgtat ggcaggcaga tgccgtctga aaaaacgctg
    43921 tcggccgcct gcctgcaaat gcgagattat atcacttgct tttggcggct gcattgattg
    43981 tttctatttt cgggatagtg ataaaatccc gacttatcca tatcttacaa ggcagaaaca
    44041 gatgaaattc ccgacccgtg ccatccattc cggctacgat tgcgacgaac acaaccgcgc
    44101 gctgatgccg ccgatttatc aaaacagtat gtttgcgctg cacgaaatcg gcgaaaatgt
    44161 gccttaccgt tattcgcgcc tgagcaaccc gacccgtcag attttggaag acaccgttgc
    44221 cgatttggaa cacggtgcgg caggttttgc gttttccagc ggtatggcgg ggattgatgc
    44281 cgtgtggcgt accttcctgc gcccgggcga taccatcgtc gcggtcgccg atatttacgg
    44341 cggcgcatat gacttattgg tcgatgttta tcaaaaatgg ggggtgaacg ttgtttttgc
    44401 cgatttgggc aatcccgata atttggacga attgctcaaa gcgcacaatg tcaaactggt
    44461 ttggctggaa atgccgtcca atccgctttt acgcttggta gacatcaaag cccttgcagc
    44521 gaaagccaaa gcagccgacg cgctggtcgg catcgacaac acctttgcca cgccgtatct
    44581 gcaacagccc ttggatatgg gttgcgattt tgcattccat tccgctacca aatatttgtg
    44641 cggccattcc gacgtattga tgggcatcgt cgttgccaaa accaaagagc tggctcagcc
    44701 tttgcacgat atgatggtgc ataccggcgc gattgcaggc ccgacggact gctggctggt
    44761 gttgcgcggc atcaaaacgc ttgccctgcg tatggaggca cactgcaaaa acgcactcga
    44821 aattgcgcgc cgtttggaag cccatcctgc cattgaaaaa gtgttccatc ccggcctgcc
    44881 gtctcacgaa cattacgcac tcgcccaagc gcaaatgccc aaaggcatcg gcggcgtggt
    44941 tacggtttat ctcaaaaacg acacgcgcga agcggcaaac agcgtgatta aaaacatgaa
    45001 actggtcaaa atgacctcca gcctcggcgg cgtggaaagt ctggtcaacc attgctattc
    45061 ccaatctcac agcggcgtgc cgcataatgt gaaaatggaa atgggcatca gggtcggact
    45121 cctgcgcttc tccatcggta ttgaagacgt agatgatatt tggaacgata tttccgccgc
    45181 actcgataca actttgtaaa ccgtaaaaaa tgccgtctga aaccatagtt tcagacggca
    45241 tttcaatatt gcgaatcaaa tatcaacgct tcagcatctt tgccgcctca atggcgtaat
    45301 aggtcaaaat cccgtccgca cccgcacgtt tgaatgccag caggctttcc aaaaccactt
    45361 tgccgccgtc cagccagccg ttggcaaccg ccgcctgcaa catcgcatat tcgcccgaaa
    45421 cctgataggc ataagtcggt acgccgaact cgtccttcac gcggcggaca acgtccaaat
    45481 acggcaaacc gggcttcacc atcaccatat ccgcaccttc ctgaatatcg agcgccactt
    45541 catgcagcgc ctcatcggta tttgcaggat ccatctgata ggtcttttta tctgcctttc
    45601 ccaaattgcc cgaactgcct accgcatcac ggaaagggcc gtagaatgca gaagcatatt
    45661 tggcggaata tgccataatc cgcgtatgga tatgtccggc atcctccaaa gcctcgcgga
    45721 tggcgccgat acgcccgtcc atcatatcgg aaggagcaac gacctgcgtg cccgcctctg
    45781 catgacataa agcctgtttc accaagactt ctacggtttc atcattcatc acgtaaccgt
    45841 tttcgtccgt cagtccgtcc tgaccgtgca ccgtataagg atcgagcgcg acatccgtca
    45901 taatgcccag ttcgggaaat ctctcgcgca aggctcggac agttgacggc acgagtcctt
    45961 cgggattgta cgcctcctgc gcacgcccgg ttttgtttgc cgtaaccacg ggaaagagtg
    46021 ccaacatcgg aataccgagc ttcaccgcct cttccgccgt aaacagcagc ctgtccaaac
    46081 tctgacgctt cacgcccggc atagaaggca catcctcctc gcgcgccgcc ccctccaata
    46141 cgaacaccgg ataaatcaaa tcatcggcgg tcagcatatg ctcgcgcatc aggcggcgtg
    46201 aaaaatcatc cctgcgcata cggcgcatac gcgaagccgg aacattgcgg taaggaaatt
    46261 gcataagccc tccaatcatc ctgtcaacaa ttcaaatcat acggaagccg ccgcaacttg
    46321 cagacggcat cgagcggtaa tcaaaatccg ttttcaccgg aaagggtttc ggcacgccga
    46381 aatgccgcgc cgaaggacag cgtgccgccc attgaaagaa caatagcaaa acaaccactt
    46441 caggctgtca ggcctcgata ttcttacgcc acaataccaa aagtttcccg atatgccgaa
    46501 caggttgcgc gtcaaccgcc tcacacaagg tattgcagat ttcgatacgc tcggcacggt
    46561 cgtcgccgaa tacgcgcact ttaatcagct catgcgccgt cagtgccgca tcggtttcct
    46621 tgatgactgc gtccgtcaga ccctgctgac cgaccatcac aacaggatgg agatggtgcg
    46681 cgcgcgcttt cagttccaaa atttctttgg tgttcaattt ggtatccgtc attttcctac
    46741 tgtttgaata aaaaggataa tgcagcattg tacgcgattt ggcacggcaa cggcaaaatt
    46801 aacgtacaat acaccgttta cattccgacc cgcgaaccac tatggctgta cgttccaaat
    46861 cctcaaaagc gtggctgcac gaacacatca acgaccacta cgtccatatg gcgcaaaaag
    46921 acggctaccg cgcccgtgcc gcatacaaac ttttggaaat caacgaaaaa gacaagacaa
    46981 tcaaacccgg cacggtactt gccgacttgg gcagcgcgcc gggaagctgg tcgcaggttg
    47041 ccgccaagct gacgggtact tccggaacgg ttttcgcctt ggacatcctg cctatggaag
    47101 ccataggggg cgtgtccttc attcagggcg acttccgcga gaacgacgtt ttggcgcaat
    47161 tcgaaacctt gttggacaac cgcccgctcg accttgtaat ctgcgatatg gcgcccaata
    47221 tgtcgggaaa cgccgtaagc gatcaggcac gcagctttta tctgtgcgaa ctggctttgg
    47281 acttcgcctc gcaacacctg aaaaccggcg gcagcttttt ggtcaaagtc tttcagggtg
    47341 caggctatca ggaatacatg gcagccatgc gcgaaatttt cggcactgtg cagacgcgca
    47401 aacccgaagc ctcgcgcaat cgctccagtg agatttattt attgggcaaa aataaacgct
    47461 gacaatacag acggcgtgct ttacaatcat ttccgtttta cacctcaatt atggagcctt
    47521 gctaagtggg gaacaccttt aaatcaatcc ttgtctgggt cgccttgggt atcggcctga
    47581 tggctgcgtt caacgctttg gacggcaaaa aagaagacaa cgggcaaatc gaatattctc
    47641 agttcatccg acaggtcaac aacggcgaag tatccggcgt caacatcgaa ggatccgtcg
    47701 tcagcggcta cctgattaag ggcgagcgca ccgacaaaag caccttcttc accaacgcgc
    47761 ccttggatga caacctgatt caaacccttt tgaacaaaaa cgtccgcgta aaagtaacgc
    47821 cggaagaaaa accgagcgcg ctgactgccc tgttttacag cctgctgccc gtcctgctgc
    47881 tgattggcgc atggttctac tttatgcgta tgcaggcggg cggcggcgga aaaggcggcg
    47941 cattctcctt cggcaaaagc cgcgcccgcc tgctggacaa agatgccaac aaagttacct
    48001 ttgccgatgt cgccggctgc gacgaagcca aagaagaagt gcaggaaatc gtcgattacc
    48061 tcaaagcacc gaaccgctat caaagcctcg gcggtcgtgt tccgcgcggc atcctgctgg
    48121 cgggcagccc gggaaccggt aaaacactct tggcgaaagc cattgcaggc gaggccggcg
    48181 tgccgttctt cagcatttcc ggttccgatt ttgtcgaaat gttcgtcggt gtcggtgcaa
    48241 gccgcgtccg cgatatgttc gagcaggcaa agaaaaacgc cccatgcatt atctttatcg
    48301 acgagattga cgcggtaggc cgccaacgcg gcgcaggttt gggcggcggc aatgatgagc
    48361 gcgagcaaac attaaaccaa ttattggttg aaatggacgg ttttgagagc aatcagactg
    48421 taattgtgat tgcggcaacc aaccgccccg acgtactcga tcctgcgctg caacgccccg
    48481 gccgcttcga ccgccaagtc gtcgtccccc tgccggacat ccgggggcgc gaacagattt
    48541 tgaacgtcca ttctaaaaaa gtgcctttgg acgaatctgt ggatttattg tccctcgcgc
    48601 gcggcacgcc cggtttttcc ggcgcggatt tggcgaacct ggtcaacgaa gccgccctgt
    48661 ttgccggccg ccgcaacaaa gtcaaagtcg atcaaagcga ttttgaagac gccaaagaca
    48721 aaatctatat gggtccggaa cgccgcagta tggtgatgca cgaagacgaa aaacgtgcga
    48781 cggcgtatca tgagtccgga cacgcgattg ttgccgaaag cctgcccttt accgaccccg
    48841 tccacaaagt aaccattatg ccgcgcggac gtgcgctggg tctgacttgg cagcttccgg
    48901 agcgcgaccg catcagtatg tataaagatc agatgttgag ccagctctcc atcctgttcg
    48961 gcggacggat tgccgaagac atcttcgtcg gacgcatctc caccggtgca tcaaacgact
    49021 ttgaacgtgc aacccaaatg gcacgcgaaa tggtaacgcg ctacggcatg agcgacaaaa
    49081 tgggcgtgat ggtttatgcg gaaaacgaag gcgaagtctt cttggggcgc agcgtaaccc
    49141 gttcgcaaaa catttccgag aaaacccagc aggacatcga cgcggaaatc cgccggattt
    49201 tggacgagca gtatcaggtt gcctacaaaa tcctcgatga aaaccgcgac aagatggaaa
    49261 cgatgtgcaa agccctgatg gaatgggaaa ccatagaccg cgatcaggta ctggaaatta
    49321 tggcgggcaa acaacccagc ccgcccaagg attacagcca caacctgcgc gagaatgcgg
    49381 acgcggcgga agataacgcg ccgcacgctc cgactcggga aaaaaccgaa gcacctgccc
    49441 cggcagacac cgcctcgaca gagtccgggc agcagcctga aaacaaggct taacttcccg
    49501 aacaaacggc agcccgcaag ctgccgtttt cccatcccgt tttcagacgg cattttccgc
    49561 tcaatgccgt ctgaaacgcc aaagccgtat cggtttttgc gaatctgcga tatggcggtt
    49621 taaattgaaa tcagaacggg gcaaggctgt accggtttaa aattaaaccg ctataatacc
    49681 gcccttcgaa tacacgcccg cattggtgat gcccgtgaaa aaagttgaaa aaaacatctt
    49741 ggtgctgcac ggcgcggaca aaatgttcga gctggtcgac aaggttgagg attacccgca
    49801 ctttctgccg tggtacagca agaccgaggt catcgggcgt agcggcaacg aactgaaggc
    49861 gcggctgttt atggattata tgcgcgttcg ccaatcgttt gccacgcaca accgcaacat
    49921 ccccggcagg gaaatccgta tggaactgct cgaaggcccg ttcaaaacct tacgtggaac
    49981 gtggaaattt atcgatttgg gcgacgatat gtgcaaaatc gaattcaatt tggaatacga
    50041 tttttccaat gccgttttgt ccgccttaat ttcccccgtc ttcaaccacc tttccgccac
    50101 gctggtcgaa gcgttcgtca aagaggcaga ccgccgttat gcttgaaatt gagattgtgt
    50161 acggtctgcc cgaccgacag gttttgaaaa ccatgcagct tgccgaggga acaaccgtcc
    50221 gcaccgccgc actgcaaagc ggtttggacg gcatatttga aaatttaaac ctgcattccg
    50281 cgcctttggg cattttcggc aaagccgtca aagacgacac gccgctgcgc gacggcgacc
    50341 gcatcgaagt gtaccgcccg ctgttgatcg accccaaaga agcgcgccgc aaacgcgttc
    50401 aaaatcaaga agaataacca tgccgtctga agccttcaga cggcattcga cagaaacacg
    50461 gaaaatatca tgtcaaacac aatcaaaatg gttgtcggct tgggcaaccc gggcaaagaa
    50521 tacgaacaga cccgccacaa tgcgggcttt tggttcctcg acgaactggc gtggaaatgg
    50581 aaggcttcgt ttaaagaaga aaaaaaattc ttcggcgaag ttgcccgcgc cgccctgccc
    50641 gacggcgatg tttggctgct caaaccggcc acgttcatga accgttccgg acaggcggtt
    50701 gccgcgcttg cacagttcta caaaatcaaa cccgaagaaa tcctcgtcgt ccacgacgaa
    50761 ctcgacatcc cttgcggacg gatcaaattc aaactcggcg gcggcaacgg cggacacaac
    50821 ggcttgaaag acattcaggc aaaactcggc acggcagact attaccgcct gcgcctcggc
    50881 atcggccacc ccggcgaccg caacctcgtc gtcggctacg tcttgaacaa accgagcgcg
    50941 gaacaccgcc ggcaaatcga cgatgccgtc gccaaatccc tgcaggccgt acccgacatc
    51001 atttccggca aatgggaaga ggcaacgcgc ttcctgcaca gcaaataatc caatgccgtc
    51061 tgaagccctt tcagacggca ttttcccgat ttccgtatcc gaacagtcat gaccgaactc
    51121 aagcagctta tccaaaccga atccatcccc gtcatcgaag aaaccctcga tttcctgctg
    51181 tacgaatgca gcatcgacga cgcaccgtcc gccgaagaag tggcacaatg gcgcgacata
    51241 cttgccgcac gcggcggcaa attcctgcgc ctgtccaaaa tctgccaaac gtggctggac
    51301 gaggaggcgg catgaagctg ccgcgcaacc gcttcagcct gctttccgca ttgtggtttg
    51361 ccggcggcat ctattcgctg ctcttcaaag ctgccgacac cgcgccgccg ccgtttccac
    51421 atttcgacaa agcagcacac cttgccctgt ttttcgcaca aatcttgctt ctggccaaag
    51481 cattcaaaac cggaaaactt cccatcccct accgcagcct gattgcgttc gccttctgtt
    51541 tttccgtcgg cagcgaatgc gcgcaggcat ggtttactgc aacgcgaacc ggcagtttgg
    51601 gcgatgtcct tgccgacctg acgggcgcag cccttgccct ctttgccgcg cgttctgcct
    51661 gccgcccgga ctaaatcggt ttatttctcc caaacaggat gaatccccct gatgcatcct
    51721 gtttttcccc ttacatatca atcccatctc ataaacaaaa ccgacaaatc gtttacaata
    51781 tatttacact acaccgaatt acaaatatac tcgaagcggt tgcagaaccg acggtttcgc
    51841 agaccgtttc ggattgcacg acacaaaaga aaaaccgatt ttgttgcctt aaaggagcat
    51901 tcatgaacct gcatgcaaag gacaaaaccc agcatcccga aaacgtcgag ctgctcagtg
    51961 cgcagaagcc gattaccgac tttaagggtt tgctgaccac cattatttcc gccgtcgtct
    52021 gtttcggcat ttaccacatc ctgccttaca gccccgatgc caataaaggt atcgcgctgc
    52081 tgattttcgt tgccgcactt tggtttaccg aagccgtcca cattaccgta accgcactga
    52141 tggtgccgat tctcgccgtc gtactcggtt tccccgacat ggacatcaaa aaggcgatgg
    52201 ctgatttttc caacccgatt atctacattt ttttcggcgg cttcgcgctt gccaccgccc
    52261 tgcatatgca gcggctggac cgtaaaatcg ccgtcagcct gttgcgcctg tcgcgcggca
    52321 atatgaaagt ggcggttttg atgttgttcc tcgttactgc ctttctgtcc atgtggatca
    52381 gcaacaccgc caccgccgcg atgatgctgc ctctagcaat gggtatgctg agccacctcg
    52441 accgggaaaa agaacacaaa acctacgtct tcctcctgct cggcatcgcc tattgcgcca
    52501 gcatcggcgg cttgggcacg ctcgtcggct cgccgcccaa cctgattgcc gccaaagccc
    52561 taaatctgga cttcgtcggc tggatgaagc tcggcctgcc gatgatgctg ttgattctgc
    52621 ccttgatgct gctctccctg tacgtcatcc tcaaacctaa tttgaacgaa cgcgtgaaaa
    52681 tcaaagccga atccatccct tggacgctgc accgcgtgat cgcgctgttg attttccttg
    52741 ccacagccgc cgcgtggata ttcggctcca aaatcaaaac cgccttcggc atttccaatc
    52801 ccgacaccgt tatcgccctg agtgccgccg tcgccgtcgt cgtcttcggc gtggcgcaat
    52861 ggaaggaagt cgcccgcaat accgactggg gcgtgttgat gctcttcggc ggcggcatca
    52921 gcctgagcac gctgttgaaa acatccggcg cgtccgaagc cttgggacag caggttgccg
    52981 ccaccttttc cggcgcgccc gcatttttag tgatactcat cgtcgccgcc ttcattattt
    53041 ttctgaccga gttcaccagc aacaccgcct ccgccgccct gcttgtaccg attttctccg
    53101 gcatcgctat gcagatgggg ctgcccgaac aagtcttggt attcgtcatc ggcatcgccg
    53161 catcttgcgc cttcatgctg ccggttgcca cgccgcctaa cgcgattgtg ttcggcacgg
    53221 gcttaatcaa acaacgagaa atgatgaatg tcggcatact gctgaacatc ctatgcgtag
    53281 tattggttgc tctgtgggct tatgctgtac tgatgtaaac catcgaccta aacaacaaga
    53341 ccgtctgaaa gaatattttt cagacgacct tgaagtttgt cgttcagaca caatttgtcg
    53401 aataattcaa aaccggattc taacgaaagg aaaacccatg attatcctgc acaccaacaa
    53461 aggcgacatc aaaatcgaac tcgatttcga caaagcccct gttaccgcca aaaacttcga
    53521 gcaatacgtc aaagacggct tctacgacgg cgtaatcttc caccgcgtca tcaaaggctt
    53581 catgattcaa ggcggcggca tggatgaaaa catgaacgaa aaagaaaccc gcgatccgat
    53641 tcaaaacgaa gcgtccaacg gcctgcccaa cgataaatac accatcgcca tggcgcgcac
    53701 ttccgacccc cattccgccg gcgcgcaatt cttcatcaac actgccgaca acgctttcct
    53761 aaacttccgt tctaaagagc tgtacggcaa aaccgtcgtc caagactggg gctatgccgt
    53821 attcggtaaa gtcgttgacg gttttgacgt tgtcgatgcc atcgaaagcg tatccaccaa
    53881 acgccacggc taccacgacg atgtgccgac cgaacctgtc atcatcatta aagccgaagc
    53941 agtataaacc ggcaatccga aagcagcctg cataaaaggc tgcttttatt tcagacggca
    54001 tgttatttga tgtaaccggg ctatgccgtc tgaaaaccgg acgcaagcct tcagacggca
    54061 taccgctatg ttaaaatacg cccgtttttc ctaaacggac acaaaggaaa ccttatggca
    54121 agcatcaccc gcgacatctt caaagcctac gacatccgtg gcatcgtcgg caaaaccctg
    54181 accgacgatg ccgcttattt catcggcagg gccatcgccg ccaaagccgc cgaaaaaggt
    54241 atcgcccgca tcgcgctcgg acgcgacgga cgcttgagcg gccccgaact gatggagcac
    54301 atccaacgcg gcctgaccga cagcggtatc agcgtactca atgtcggcat ggttaccact
    54361 cctatgctct acttcgcagc cgtcaacgaa tgcggcggca gcggagtgat gattaccggc
    54421 agccacaatc cgcccgatta caacggtttc aaaatgatgc tcggcggcga cacgctcgca
    54481 ggcgaagcca ttcaagaact tttagctatt gttgagaaag acggttttgt tgccgccgac
    54541 aaacaaggca gcgtaaccga aaaagacatc tccggcgcat accacgacca catcgtcgga
    54601 cacgtcaaac tcaaacgccc gataaacatc gccatcgacg cgggcaacgg cgtgggcggc
    54661 gcgtttgccg gcaaactcta caaaggtttg ggcaacgaag tgaccgaact tttctgcgaa
    54721 gtggacggca atttccctaa tcaccaccct gatccttcca aaccggaaaa cctgcaagat
    54781 ttgattgccg cgctgaaaaa cggcgatgcc gaaatcggct tggcgtttga cggcgatgcc
    54841 gaccgcttgg gcgtggttac caaagacggc aacattattt atcccgaccg ccaactgatg
    54901 ctgttcgccc aagacgtttt gaaccgcaat cccggcgcga aagtcatttt cgatgtcaaa
    54961 tccacacgcc tgcttgcccc gtggattaaa gaacacggcg gagaagccat aatggaaaaa
    55021 accggccaca gcttcatcaa atccgctatg aaaaaaaccg gtgcactggt tgccggcgaa
    55081 atgagcggac acgttttctt taaagaacgc tggttcggct tcgacgacgg cctgtatgcc
    55141 ggcgcacgcc tcttggaaat cctgtccgcc tccgacaatc cgtccgaagt gttggacaac
    55201 ctgccgcaaa gcatttccac gcccgaactc aacatctccc tgcccgaagg cagcaacggg
    55261 catcaagtta tcgaagaact cgccgccaaa gccgaatttg aaggcgcaac cgaaatcatc
    55321 accatcgacg gcctgcgcgt tgaatttccc gacggcttcg gtctgatgcg tgcttccaat
    55381 accacgccga ttttggtgtt gcgttttgaa gcggatacgc aagcagccat cgagcgcatt
    55441 caaaaccgat tcaaagccgt catcgaaagc aatccgcatt taatctggcc tctgtaaaaa
    55501 tagaaaaaat gccgtctgaa actttcagac ggcatttttc acaagtagcc aatcaaatct
    55561 tggcagattg gatttggctt aaaaatctcc gcgtccgttc gtgtttgagg tggtcgaaca
    55621 actctttcgg gctgccctgc tctacgataa cgccgccgtc catcacgacg acgttcgtgg
    55681 caacttccag cgtgaacttg atttcgtggg taacgacgac catcgtccaa ccttcccgcg
    55741 ccaattcctt catggcgtcc aacacgtctt gcaccaactc ggggtccagc gcggaagtgg
    55801 gttcgtcaaa cagcatcaat tcaggctgaa tcgccagtgc gcgggcgata ccgacacgct
    55861 gctgctgacc gccggaaagc tggtagggat acaaatccac tttatcgccc aagccgactt
    55921 tttccagcag tttcaaagcc tcttcgcgcg cttgggcggc aggcttgccc tgtacggcaa
    55981 ccggcccttc catcacgttt tccaacacgg ttttatgcgg gaagaggttg tattgttgga
    56041 ataccattcc ggacttgcgg cgcagtgcca aaatatcgtg tttgcttgtt tttttggaaa
    56101 aatcaatgcg taacggccgc gcgttgtcga actcgatttg tccgtcttcg ggcatttcca
    56161 acgcgtttag gcagcgcaga aatgttgttt tacccgagcc ggaaggcccg aggatgacga
    56221 ccacctgccc tttgcccaca tccaaatcga tgccgcgcaa aatggtgttt tcgccaaagg
    56281 tcttatggat attgcggatt ttaatcatga caactcctta tttggcgaca taacggtcga
    56341 aacgtttttc caaacgcgcc tgaatcaaaa acagcacttt acagaaacac caataaacca
    56401 atgcagcttc gatatagaca ggcaaaaagt cataagtgcg gtttgccgtt tcctgtgcga
    56461 cacggaaaag ctccgttacc gttaccacgg cggcaagcga ggtgtttttg aacaagccga
    56521 taaactcgtt gctcaacggc ggaacggcga cgcggaatgc ctgcggtgcg acgatgcggc
    56581 ggaacgtctg catataggtc ataccgatgg agaaacctgc ttcccactgc cctttcggca
    56641 cggacaaaat cgccgcgcgt atggtttcgg aagcgtatgc gccgacattg agcgaaaagc
    56701 cgatgatggc ggcgggaatc ggattgatat agatgccgac ggacggcagc ccgtaaaaca
    56761 caatcacaag ctgaaccaac agcggcgtac cgcgaacgac ggaaatataa aattccacca
    56821 gcttcaacaa gcatttttgg aaaataccgc cggaaggcat gattcttacc aaagcaacgg
    56881 ctacggcaat aatcatgccg ataacgaaag aagcgatcgc caaaggcaaa gacactgtaa
    56941 agccggcttt aaccatgggc caaaacgcgc tgatgagcat atcagcgcgt gtttccgtca
    57001 taaacggcag agaggcaagg aaattattga acactgatgt cttttccgaa gaattgttcg
    57061 cccagttttt tcaacgtgcc gtcggctttc agctcgttga ttgccgtgct gaattttgcc
    57121 acggcctcgt cattgccctt gttgacaatc agaccggaac cgactttttc atcggcaggc
    57181 gcggaccaca cgattttcac ccccgcattc gggtttttct tcagatagtc caaaaccgcc
    57241 aattcatcgt tcaacgtcgc atcggcgcgt ttttgttcaa tcagggtcag cgattgcgcc
    57301 aaaccgtcca ccggcacgag ttccgcacct gcggctttgg ctttttcgcc gtagttgctg
    57361 gtcagggatt gcgcggtttt cacgcctttg atgtcggcta tggatttaat gttgctgtcg
    57421 ttatgcgcaa ccaaaaccgc accgctccag ctgtaaggtt cggatttgtc aaatgtcgcc
    57481 tggcgttcgg ggctggtcag gccgacttgg tttgccacca cgtcgaaacg tccggctttc
    57541 aaacccgcca tcatcgaatc ccattgcgtt tctttaaact cgacttttac gcccagtttt
    57601 tccgccacgg cgcgggttac ttccacgtca taaccggtca gtttgccgtc tttgtcgtgg
    57661 taggtaaacg gtgcgtaagt gccttccgtg ccgacggtaa ccgtgccttt attgttgatg
    57721 cgctcgatta aagaaccgga aattgccgat tgtgcaggcg cggaagatgc tccgctgtcg
    57781 ccttccgaac cgccgcaggc cgccaaaacc aatgcggcaa tgccgccgag tacgaatttt
    57841 ttcaacatca ttccctcctg taaaaagtgt gcaagtgcgg cattctaagt gaaattgcgg
    57901 caaacacaaa gaatactttt tttatatggt tattccaaat cggaatggga aagatatgcc
    57961 gaagccgccg gaagcggaaa taaagggggc gggaaaggcg gcgggcggca cgaaaccgaa
    58021 aagcggcatc aggtatgcaa acggcggatt cacgcacgcc gttccggcaa aaatccgcct
    58081 gccgattcgg tgctataatc cacccgaaac agatgacacc aaaaaggaac atctcatgaa
    58141 cacgccccaa tccgccatca ttcccgacca cgcccaagcc ggcatcttta tcgaagccga
    58201 cttcgccgcc aaccgcctca acgatattaa agccgcctgc cgcgcttcgc tcgacgcgtt
    58261 atccgccttg aaagcccgct ttccggacga tattttgggt ctgaccatcg ccttcggcag
    58321 caaagcttgg gcaacattcg gacacacgga cgaaggcagt gaaatcaaac ccttccccga
    58381 aatgggcaac gggcttgcgc cgtccacgca gcacgatatg tccatccaca tccaatcctt
    58441 ccgccaaaac gccgcctacg cgcttgccca atccgttttg ggcgcgttcg gcgacagcat
    58501 atgcgtcgcg tccgaagaac acggtttgcg cctgtatcag gatcgcgggc tggacggttt
    58561 cgtcgacggc acggaaaacc cgcaaggcga tgaaacaatc cgcgaagtcg ccatcatccc
    58621 cgaagggctg cccgatgcag gcggcagcta tgtcctgctg caaaaatacc tgcacgattt
    58681 gaaaaaatgg gatgccgtcc ccgtcgccga acaggaggcc tcggtcggac gcagcaagga
    58741 aaccgacgac gaattcagcc gcgatgtccg cctgcccgat tcgcacctcg gccgcgtcaa
    58801 cctgaaagaa aacggagtcg gcctgaaaat cgtccgccgc agcctgccct tcggcaaaat
    58861 cagcggcgaa cacggtttga tgtttaccgc atactgccgc acgctgcata atatcgaagc
    58921 gcaattattg agtatgttcg gcgacacgga cggcaaaacc gacctgctgc tccgacacct
    58981 ctccgccgcc gtttcgggcg gatactacta cgcgccgtct gtcgaacgcc tgcaaaacct
    59041 ctaagccgga cgatgccgtc tgaaaccctt tttcagacgg cattttcccg ttttaccgtc
    59101 caaccgtttt catacacgct atgtccgcac ccatccgagc attcgacccg ctgaccgccc
    59161 ccatttccgg caccaacctg attgaggcct ctgccggcac cggcaaaacc tacggcattg
    59221 ccgccctgtt tacgcgcctg atcgtattgg aacaaaaaag cgtcgagcgc gtattggtcg
    59281 ttaccttcac caaagccgcc actgccgagc tgaaaacacg cctgcgcgcg cgtttggacg
    59341 atgtgttgca ggttttggaa agcaaagaaa ttgccgaact tggagacgac acgctttcag
    59401 acggcattgc cgcctactgc gccgaacacc acgaaggcga caccttcctg cccgcactct
    59461 tggaacaggc tttgcaaaaa gaaagccgga cgcgtctgat tgtccgcctc aaggccgcca
    59521 tcgggcaatt cgacaacgcc gccatctaca ccatccacgg cttctgccag cgcatcctgc
    59581 gcgactacgc cttcctgtgc caagcaccgt tcgatgtcga actgaccgaa gaagacggcg
    59641 accgcctgct tgtcccggct caagattttt ggcgggaacg cgtcagcggc gacccggtgc
    59701 ttgccgcatt ggcgtttaaa cgcaaagctg tgccgcaaac cgtccttgcc caaatccgcg
    59761 cctacctgtc ccgcccgtac ctgaatttcc gccgtccgca ggcggatttg aaacaggcgc
    59821 agcgcgacgc cgaaacctca tggcaaaccg tctgccgcct gctgcccgag ctggaagccg
    59881 gcttttggcg catccatccc gacctcaacg gcaacagtta ccgcaaaaac agcttcggca
    59941 acctttttaa agaactggca caaaaatccg ccgccggaca actgccctgt ctggacaaag
    60001 acacgcacga acggctcttg aaactctcat ccgacaaact cgaagccgga ctgaaaaaag
    60061 gcaaaacgcc cgatgcggca gtatttgccg aattgcagaa actggcagac ttcgggcgcg
    60121 atttgaacgc actcgaagaa gcggaagaaa caacaatgat ccggctgcaa ctggatttaa
    60181 tcgaatatct caacagcagc cttgccgaaa tgaaaaaatc gcgccgcgaa cgcggtttcg
    60241 acgacctgct gctcgatgtc cacaccgcgc tgaccgacaa tccgcacgcc gaaacccccg
    60301 cccgcgccgt tgccgaaaac tgggaaatcg cgctgatcga cgagttccaa gacaccgacc
    60361 cgctgcaata cgaaatcttc caaaagattt tcatcgcccg aaaccgcccg ctgttcctcg
    60421 tcggcgaccc caaacaggcg atttacagct ttcgcggtgc ggacatttac gcctaccttc
    60481 aggcggcgga agacgcgcgg caccgctaca cgctcgccac caactaccgc agccacgccg
    60541 cgcttatcgg cagcatcggc gcgctgttcc gcctcaaaga acgcccgttc gttttggaaa
    60601 acatcggcta ttcggaagtc ggtgcggcgc gtgccgaaag caggttgtcg cccaaacgtc
    60661 ctgccgtaca gctccgttgg ctgcacgaga acgacaatga aaaagccaac aaagacgttt
    60721 tgcgccgccg tgccgccgac tattgcgccg acgaaatcgc ccgcgcgctc aacgaagcct
    60781 ccggaggccg tctgaatttc aaaggccgcc cgttgcagtc gggcgatatt gccgttctgg
    60841 tacgcacgca caacgaggcg gtgatgattt ccgccgcact gaaaaaacgg caggtgcaaa
    60901 gcgtcctgct ttcgcgcgaa tccgtgttcg cctcgcccga agccgccgcc ctgtccgcgc
    60961 tcatcggctt ctggctcgaa ccgcgccgcg ccggaacgct gcgctttgtc ctgacaagcg
    61021 gcatattcgg ttatgacgcg cagcaattgc acgacttcaa ccaaaacgaa agcgagattt
    61081 tgcattgggc ggaatctgcc cgaaccgcgc tcgacatatg gcaaaaatac ggcattttcg
    61141 ccgctatgca gcagttttcc caaacacacg gcatcgaaac gcgcctctta agccggaaca
    61201 acgggcgcag cctgaccaat tatttccaac tgctcgaact gcttgccgcc gaagacgcgc
    61261 aaaaccgcaa ccccgccgca ctgcacaaat ggctgcgcga tcaaatcagc ctggccggca
    61321 acaacggcgg cgacaaccgc gccatccgtt tggaaagcga cgaagatttg gtcaaaatcg
    61381 ttaccatgca cgcctcgaaa ggtttgcagt atccgctggt gtactgcccg tttgcgtggg
    61441 acgcgcaaga taccggaccg tccgactggc aaatcctcca ccaaggcgca aaccgggccg
    61501 aacttttggc aaaggcgcaa ctgtcggaaa gcgaacagaa gcaatacgcc gacgaagaaa
    61561 tggcggaacg cctgcgcctg ctttatgtcg cgctgacgcg tgccgaggaa cagctcaaca
    61621 tctacgccgc ctattcttcc gataccgccg acaaccccct tgcctacctg atcgaaggct
    61681 tgccgcaaga cagccgcgaa accgtccgcc gtacctatgc gtgtgaaaaa gacggcataa
    61741 cgatgctcaa acgcaactgg cggcgcgtgg cggacaatgc cccggccggc acggatttcg
    61801 ccttcacaga agatgcgccg ccccctgccg cctatcgcgg caacgccgat caagccgccg
    61861 aatttgccgc aaacagcatt cccgaacgcg gattccgatt tgtccgccac accagcttta
    61921 ccgccttaag ccgccatacc caaacgcccg acggcggcga agaagatgcc tgcccgtcct
    61981 tggatgccgc cgaaacctcg gtgccggcga tgccgtctga aacgccgacg gcttcagacg
    62041 gcatatcgat acacgacttt ccgaaaggca cgcaggcggg gctgtgcctg cacgaaattc
    62101 ttgaagattt caaattcgga caagcagccg ccgaacagga aaccctcatt gccgacaagc
    62161 tgaaaaaata cggttttgaa gaaatatggc tgcccgccgt tgccgaaatg gcggaagcct
    62221 gccgcaaaac gccgctgact ggggcatacg gcctgtccga catcccgccc gggtgccgcc
    62281 gtcccgaaat gggctttacc ctccacaccg aagacttcgg cctcaaacgg ctgcgcgact
    62341 ggcttgcccg cgacgacatc aggctgcccg aagtctgccg tgccgccgcc gagacgctcg
    62401 acttccacac cgtcaacggc tttttaaacg gctttatcga tatggtctgc caagaccccg
    62461 acggcaatat ctgcatcatc gactacaaat ccaaccacct atccgcctac acgcggcagg
    62521 cgatggacga agccgtcgcg caccagcact attaccttca ggcactgatt tacgccgttg
    62581 ccgccgcgcg ctacttcaaa ctgcgcggac aaccgcccgc cgccgtttcc gtccgctacc
    62641 tgtttttgcg cggattggat ggcaaaggcg gcggcgtttg gcgttgggac atagatgccg
    62701 ccgctttgga acagataaaa taacttcccg tcccttaaga cgacaaccgc aggagaaccc
    62761 tatgaatatc aaacaattga ttaccgccgc actcattgcc tcagccgcct ttgccacgca
    62821 ggcagccccg caaaaacccg tatccgccgc ccaaaccgcg caacattcag ccgtttggat
    62881 cgatgtccgt tccgaacagg aatttagcga aggtcatttg cacaacgcgg tcaacatccc
    62941 cgtcgaccaa atcgtccgcc gcatatacga agccgcgccc gacaaagaca cgccggtcaa
    63001 cctctactgc cgcagcggac ggcgtgccga agccgccctt caagagctga aaaaagcagg
    63061 ttatacaaat gttgccaatc acggcggtta tgaagacctg ctcaaaaaag ggatgaaatg
    63121 acgcaatgcc gtctgaaagg acaggctttc agacggcata cggcgttccg gcacgcggca
    63181 cggcatcagg cgcggcgcgt gccgccccgt ccgaagcccg ataaacaaac cgataaggaa
    63241 atacgatgaa acacatactc cccctgatcg ccgcatccgc actctgcatt tcaaccgctt
    63301 cggcacaccc tgccggcaaa ccgcccaccc aaaacgaaac cgctatgacc acgcacaccc
    63361 tcacctcgaa atacagtttt gacgaaaccg tcagccgcct tgaaaccgcc ataaaaagca
    63421 aagggatgga catttttgcc gtcatcgacc atcaggaagc ggcacgccga aacggcctga
    63481 ccatgcagcc ggcaaaagtc atcgtcttcg gcacgcccaa ggccggtacg ccgctgatgg
    63541 tcaaagaccc cgccttcgcc ctgcaactgc ccctgcgcgt cctcgttacc gaaacggacg
    63601 gcaaagtacg caccgcctat accgatacgc gcgccctcat cgtcggcagc cgcatcagtt
    63661 tcgacgaagt ggcaaacact ttggcaaacg ccgaaaaact gatacaaaaa accgtaggcg
    63721 aataagcccg tcgccgacat atggcggatg gaaatcaaat gcacacgccc ctcattcccc
    63781 cgaagtcggg aatccaagcg gttgggttac cgtgatttcc aatcattgcc gaaacggacg
    63841 attccggatt cccgccttcg cagggatgac gggcgtatgc gttttgccat atcacaggca
    63901 actttgccgc aaatcttatc tgtcgccggt atgccttctt gtttcaatcc gccacaaacc
    63961 aaatgccgtc tgaaacctga tttcagacgg catttccgtt tattccctgc tgtcgcggca
    64021 aatatcgatg gcttcctgca aacttgaaac gccgtggatt ttcaggttcg gaaactcttt
    64081 ggcattgcgc ggcatattgg ctttggggac gatggcgcgt ttgaagccga gcttttccgc
    64141 ttctttgagc cgctcttgtc cgcgtgcgac ggggcggact tcgccgctta ggccgatttc
    64201 accgaagacc acggtttttt caggcatagg gcggttgcgg aagctggaaa gcatcgcgag
    64261 gatgaccgcc aaatccgccg ccggttcgcc gattttcacg ccgccgacgg cgtttaaaaa
    64321 aacatcctga tcgaaacagg cgatgccgcc gtgccggttt aacacggcaa gcagcatcgc
    64381 aaggcggttt tgttccaatc cgacgctgag gcgtttgggt gtgaagccgt gcgcgtcatc
    64441 gaccaatgcc tgaatttcga ccaaaagcgg gcggctgcct tcctgtgtaa ccaaaacgca
    64501 cgaaccgggc gtgtcgtcgc ggtagctggc gaggaagatg gcggacgggt tggatacgcc
    64561 tttcaaaccg ttttcggtca tcgcgaacac gcccaattcg tttgccgcgc cgaaacggtt
    64621 tttgatggcg cgtatcatgc ggtagttgga atgttggtcg ccctcgaaat acagcacggt
    64681 atcgaccata tgttccagca cgcgcgggcc ggcaatcgct ccgtctttgg taacgtgtcc
    64741 gaccagtatc atggcgatac ccatttgttt cgccatacgc gtcagttggg cggcacattc
    64801 gcgcacctgc gacacggaac cgggggcgga cgtgatttgg tcggaataca tggtttggat
    64861 ggaatcgata acgacgactt cgggctgatg ctgtttcaag gccgcctgaa tcgcttccat
    64921 gcggatttcg gcaagcaggt ttacgccttc ggcgtgcagt tccaaacgtt gcgagcgcag
    64981 ggcgacctgt tgggcggatt cttcgccgga aacgtacagc actttgcggc tttgcgccat
    65041 tttggcgatg gtttgcaaca acagcgtgga tttgccgatg ccgggatcgc cgccgagcag
    65101 gatgaccgcg ccatcgacca aaccgccgcc caatacgcgg tcgagttcgc ccataccggt
    65161 cggattgcgc ggcacttcgg cagcggtaac ggcggagagg gattggacgg tcgaagtgtc
    65221 cgccgcccaa gattggaatc gggcgttttt cggctcgggc gcggcaaagc tttcctgaag
    65281 cgtgttccac tcgccgcaat gcggacattt gccctgccat ttcggggaag tgccgccgca
    65341 ttcggtgcat tggtaaaggg ttttaagcgt ttttgccatg atgttttccc tatgggattt
    65401 ttgaagtgta aaaaaatcgt ctgaaacttc ttttctaaaa aattttttat tattagtttg
    65461 ttaattttga ttacaagacc atatcacgat acaaacaagg gtggctttct gttattttgc
    65521 ggcataatga cggaaaatac aaaacaggaa tcccaaacaa tgactttccc attaaatata
    65581 atggtaga
//
LOCUS       DAXFWM010000003        63421 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000003 DAXFWM010000000
VERSION     DAXFWM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 63421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63421
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.003"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(200..1039)
                     /locus_tag="ACFI8B_000152"
     CDS             complement(200..1039)
                     /locus_tag="ACFI8B_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC3833477.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="ACFI8B_000153"
     tRNA            1324..1399
                     /locus_tag="ACFI8B_000153"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="ACFI8B_000154"
     CDS             complement(1464..2657)
                     /locus_tag="ACFI8B_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC3833478.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACFI8B_000155"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACFI8B_000155"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC3833479.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="ACFI8B_000156"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="ACFI8B_000156"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC3833480.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5103..5528
                     /locus_tag="ACFI8B_000157"
     CDS             5103..5528
                     /locus_tag="ACFI8B_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC3833481.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /locus_tag="ACFI8B_000158"
     CDS             complement(5602..7587)
                     /locus_tag="ACFI8B_000158"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC3833482.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVAELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8202)
                     /locus_tag="ACFI8B_000159"
     CDS             complement(7654..8202)
                     /locus_tag="ACFI8B_000159"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC3833483.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="ACFI8B_000160"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="ACFI8B_000160"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC3833484.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHMLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="ACFI8B_000161"
     CDS             complement(9712..10710)
                     /locus_tag="ACFI8B_000161"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC3833485.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACFI8B_000162"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACFI8B_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC3833486.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="ACFI8B_000163"
     CDS             complement(12001..12771)
                     /locus_tag="ACFI8B_000163"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC3833487.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="ACFI8B_000164"
     CDS             complement(12774..13766)
                     /locus_tag="ACFI8B_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC3833488.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WDVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="ACFI8B_000165"
     CDS             complement(13833..14516)
                     /locus_tag="ACFI8B_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC3833489.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACFI8B_000166"
     CDS             complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACFI8B_000166"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC3833490.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALIAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLAAVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16169..17023)
                     /gene="folP"
                     /locus_tag="ACFI8B_000167"
     CDS             complement(16169..17023)
                     /gene="folP"
                     /locus_tag="ACFI8B_000167"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC3833491.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEEWARVEPVLAEAAGWGVPVSLDTR
                     RTVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKY
                     QDVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     LPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKV
                     WEALGVNR"
     gene            complement(17163..18056)
                     /locus_tag="ACFI8B_000168"
     CDS             complement(17163..18056)
                     /locus_tag="ACFI8B_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC3833492.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18147..20249
                     /locus_tag="ACFI8B_000169"
     CDS             18147..20249
                     /locus_tag="ACFI8B_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC3833493.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPRLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20313..21791)
                     /gene="ubiD"
                     /locus_tag="ACFI8B_000170"
     CDS             complement(20313..21791)
                     /gene="ubiD"
                     /locus_tag="ACFI8B_000170"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC3833494.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21811..22326)
                     /locus_tag="ACFI8B_000171"
     CDS             complement(21811..22326)
                     /locus_tag="ACFI8B_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC3833495.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSDQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22333..22650)
                     /locus_tag="ACFI8B_000172"
     CDS             complement(22333..22650)
                     /locus_tag="ACFI8B_000172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833496.1"
                     /translation="MYWYPVLVNLMMLAVFGSSLFAGQTVIEKLARLQQPDLPEKAVR
                     YTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLV
                     LKV"
     gene            complement(22717..22869)
                     /locus_tag="ACFI8B_000173"
     CDS             complement(22717..22869)
                     /locus_tag="ACFI8B_000173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833497.1"
                     /translation="MKPGFFGHIFLTVLSIAYPALWYSGLNLNRYGVASPCRTICTVC
                     GFAALS"
     gene            complement(22945..23028)
                     /locus_tag="ACFI8B_000174"
                     /pseudo
     CDS             complement(22945..23028)
                     /locus_tag="ACFI8B_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23031..23773)
                     /locus_tag="ACFI8B_000175"
                     /pseudo
     CDS             complement(23031..23773)
                     /locus_tag="ACFI8B_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23974..24401
                     /locus_tag="ACFI8B_000176"
                     /pseudo
     CDS             23974..24401
                     /locus_tag="ACFI8B_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            24439..25168
                     /gene="fabG"
                     /locus_tag="ACFI8B_000177"
                     /pseudo
     CDS             24439..25168
                     /gene="fabG"
                     /locus_tag="ACFI8B_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            25162..26507
                     /locus_tag="ACFI8B_000178"
                     /pseudo
     CDS             25162..26507
                     /locus_tag="ACFI8B_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1,RefSeq:WP_003762767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            26561..26636
                     /locus_tag="ACFI8B_000179"
     tRNA            26561..26636
                     /locus_tag="ACFI8B_000179"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26594..26596,aa:Ile,seq:cat)
     gene            26711..27469
                     /locus_tag="ACFI8B_000180"
     CDS             26711..27469
                     /locus_tag="ACFI8B_000180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3833498.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27470..28534
                     /locus_tag="ACFI8B_000181"
     CDS             27470..28534
                     /locus_tag="ACFI8B_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3833499.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28768..30105
                     /locus_tag="ACFI8B_000182"
     CDS             28768..30105
                     /locus_tag="ACFI8B_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3833500.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNFLGIL"
     gene            complement(30280..31293)
                     /locus_tag="ACFI8B_000183"
     CDS             complement(30280..31293)
                     /locus_tag="ACFI8B_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC3833501.1"
                     /translation="MGRHFGRRRFLTAAAVAVAGAAVSFLPNPFAAGGEKRNMDKKRD
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLCRDIHALTEGAFDPTVQALWKLYADYFAARPDAETPPENS
                     VKETLKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQGYITDKVVALLKANGVPAALVD
                     MGEIRGFDTNGRRMWNVGIRNPDDEEGVLANITVKDKAFATSGGYGTVMDKAGRLTHL
                     FDPRTGVSTPRYKSMSVMADDAAVADALSTAFSVMDLPLIRSVAESRRLKVRLAMPDN
                     IVD"
     gene            complement(31302..32096)
                     /locus_tag="ACFI8B_000184"
     CDS             complement(31302..32096)
                     /locus_tag="ACFI8B_000184"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC3833502.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            32520..33854
                     /gene="gdhA"
                     /locus_tag="ACFI8B_000185"
     CDS             32520..33854
                     /gene="gdhA"
                     /locus_tag="ACFI8B_000185"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC3833503.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            33949..34055
                     /locus_tag="ACFI8B_000186"
                     /pseudo
     CDS             33949..34055
                     /locus_tag="ACFI8B_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(34093..34872)
                     /locus_tag="ACFI8B_000187"
     CDS             complement(34093..34872)
                     /locus_tag="ACFI8B_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC3833504.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            35222..35428
                     /locus_tag="ACFI8B_000188"
                     /pseudo
     CDS             35222..35428
                     /locus_tag="ACFI8B_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            35434..35626
                     /locus_tag="ACFI8B_000189"
                     /pseudo
     CDS             35434..35626
                     /locus_tag="ACFI8B_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35827..37230)
                     /gene="mtrE"
                     /locus_tag="ACFI8B_000190"
     CDS             complement(35827..37230)
                     /gene="mtrE"
                     /locus_tag="ACFI8B_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC3833505.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(37283..40486)
                     /gene="mtrD"
                     /locus_tag="ACFI8B_000191"
     CDS             complement(37283..40486)
                     /gene="mtrD"
                     /locus_tag="ACFI8B_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC3833506.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMTLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            complement(40498..41736)
                     /gene="mtrC"
                     /locus_tag="ACFI8B_000192"
     CDS             complement(40498..41736)
                     /gene="mtrC"
                     /locus_tag="ACFI8B_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC3833507.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            41986..42618
                     /gene="mtrR"
                     /locus_tag="ACFI8B_000193"
     CDS             41986..42618
                     /gene="mtrR"
                     /locus_tag="ACFI8B_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC3833508.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     DALYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIHYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            complement(42711..42923)
                     /locus_tag="ACFI8B_000194"
     CDS             complement(42711..42923)
                     /locus_tag="ACFI8B_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833509.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            43255..44823
                     /gene="mtrF"
                     /locus_tag="ACFI8B_000195"
     CDS             43255..44823
                     /gene="mtrF"
                     /locus_tag="ACFI8B_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC3833510.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMAASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFIALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVMKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            complement(44893..48099)
                     /gene="recC"
                     /locus_tag="ACFI8B_000196"
     CDS             complement(44893..48099)
                     /gene="recC"
                     /locus_tag="ACFI8B_000196"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC3833511.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEEETPVFEEGGRDTLLHALQTDIQNLKMPSETVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSGE
                     LGKLWQQSFQTAAKQIDTSVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAYG
                     KPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIVQSGETEVLAEIAQDRALQLLSEWMA
                     FFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVAL
                     VFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(48200..49609)
                     /locus_tag="ACFI8B_000197"
     CDS             complement(48200..49609)
                     /locus_tag="ACFI8B_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC3833512.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            complement(49953..51272)
                     /gene="ccoP"
                     /locus_tag="ACFI8B_000198"
     CDS             complement(49953..51272)
                     /gene="ccoP"
                     /locus_tag="ACFI8B_000198"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFC3833513.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLYVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPAAEPAPSAPAGAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            complement(51296..51466)
                     /locus_tag="ACFI8B_000199"
     CDS             complement(51296..51466)
                     /locus_tag="ACFI8B_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC3833514.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            complement(51471..52082)
                     /gene="ccoO"
                     /locus_tag="ACFI8B_000200"
     CDS             complement(51471..52082)
                     /gene="ccoO"
                     /locus_tag="ACFI8B_000200"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC3833515.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            complement(52109..53542)
                     /gene="ccoN"
                     /locus_tag="ACFI8B_000201"
     CDS             complement(52109..53542)
                     /gene="ccoN"
                     /locus_tag="ACFI8B_000201"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC3833516.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            53868..55616
                     /locus_tag="ACFI8B_000202"
     CDS             53868..55616
                     /locus_tag="ACFI8B_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC3833517.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKIEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            55681..56109
                     /locus_tag="ACFI8B_000203"
     CDS             55681..56109
                     /locus_tag="ACFI8B_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC3833518.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(56610..57044)
                     /locus_tag="ACFI8B_000204"
     CDS             complement(56610..57044)
                     /locus_tag="ACFI8B_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC3833519.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(57047..57709)
                     /gene="exbB"
                     /locus_tag="ACFI8B_000205"
     CDS             complement(57047..57709)
                     /gene="exbB"
                     /locus_tag="ACFI8B_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC3833520.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(57775..58626)
                     /locus_tag="ACFI8B_000206"
     CDS             complement(57775..58626)
                     /locus_tag="ACFI8B_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC3833521.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            58882..59520
                     /locus_tag="ACFI8B_000207"
     CDS             58882..59520
                     /locus_tag="ACFI8B_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC3833522.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(59592..60233)
                     /locus_tag="ACFI8B_000208"
     CDS             complement(59592..60233)
                     /locus_tag="ACFI8B_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC3833523.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            60376..62589
                     /locus_tag="ACFI8B_000209"
     CDS             60376..62589
                     /locus_tag="ACFI8B_000209"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC3833524.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            complement(62653..63030)
                     /locus_tag="ACFI8B_000210"
     CDS             complement(62653..63030)
                     /locus_tag="ACFI8B_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC3833525.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGIGIIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFR
                     VMLRYIFTEIDIVNVRFDYYNKK"
     gene            complement(63036..>63421)
                     /locus_tag="ACFI8B_000211"
     CDS             complement(63036..>63421)
                     /locus_tag="ACFI8B_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin C-terminal domain-containing protein"
                     /protein_id="HFC3833526.1"
                     /translation="NTIVKPTPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPPRK
                     TPSTPVYGNDSEAYAAAKKLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEA
                     SSPKNLNRKETRNGTFDKNLNRIGD"
BASE COUNT        14863 a        17936 c        15981 g        14641 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcagg ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttgttcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacaggg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacatag
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgctgg cgcggcggca cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaagc tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcgacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcaggccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg cgacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcggacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaagtt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 ggctacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt attttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc catgaatgtt cgcgcacgcg
     8101 cttaccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag acatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgactttga taccgcacgt tttgacgcac tggaagaacg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactctcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacat gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtagc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg cgcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt cccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg acttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggc gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gcgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgccccgcc
    14641 ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt
    14701 ttgcctaaat gccgccgatg gcggcggcga tacgctccgc cccgtcgcgc gcccaatcgg
    14761 cctgacgcgc ctcgaccatc acgcgcacga cgggttcggt tcccgaagcg cgcaacacga
    14821 cacgcccttt gccttcgagt tctttttcca cttcggccaa cacgtctttc gaagcctcct
    14881 gccatttctg acccttttgg atgcgcacgt taatcatcgt ttgcggatac ggctgccaat
    14941 cggcgcaaac ggcggcgagg tcttggttca ggatttgcag cgccgccaag acttgcagcg
    15001 cggaaataat gccgtcgccg gtgttatgtt tgtccatgca caaaatatgg ccgctggctt
    15061 cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg tcgccgactt
    15121 tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc atattggtca
    15181 tgaccgttcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct ttggcgatga
    15241 cgtaaatcag gctgtcgccg tcgtaaacct gcctgttttt atcgaccatc atcaggcggt
    15301 cgccatcgcc gtccaaggca ataccgtaat cggcttcgtt ttgcaacacg gcggcttgga
    15361 gggttttggt atgggtcgcg ccgcattttt cgttgatgtt gtagccgttg ggttcgttgc
    15421 cgatgctgac gacctgtgcg ccgagttcgt ggaacacttt gggtgcaacg ccgtatcctg
    15481 caccgttggc ggtatcgata accaacttca aaccccgaag gtcggaatgg ccggggaagg
    15541 tggatttgca aaactcgata tagcggtcgt ccgcaccgct gatgcggcgg gcgcgtccga
    15601 gacgtgcgga cggttgggtt ttcatttctt catcgatttt ggcttcgatt tccaactcga
    15661 cttcatcgga aagtttcacg ccgccttcgg caaagaattt gatgccgttg tcggaatagg
    15721 tgttgtgcga cgcggaaatc atcacgccgg cggacaggcg caatgcgcgg gtcagataag
    15781 ccacaccggg cgtgggcaac ggcccggtct gtaccacatt cacacccgcc gccgtaaagc
    15841 ccgctatcag cgcggcttcc agcatatagc ctgaaatgcg cgtgtctttg ccgatgagga
    15901 cggtcggttt ctgatcggta tcgtgctgca ccagcacctg ccccgccgca tagccgagtt
    15961 tcaatacaaa atcgggcgta atcggaaact gaccgacttc gccgcgcacg ccgtccgtgc
    16021 cgaaatattt ttttgccatc atttgctccg tgaatgtgaa ccgttgtccg agattataca
    16081 gtcagtttgc gccctgctgc ctgcaccgtt gatgccgtct gaagccgtcc cgcccttttc
    16141 agacggcatt cggcgcaaaa accgccgttt accggttcac gcccaacgct tcccacacct
    16201 tcaacgcgtc cgccgtcgcc ttcacatcgt gcacgcgcac gatttgcgct ccgcgtgcca
    16261 cggaagccaa agccgctgcc acgctgccgt gtacgcgcgc cgccgcgtcc gcctcaccgg
    16321 tcagctcacc tatcatgctt ttgcgcgaca caccgatcag cagcggcaaa ccggtttccg
    16381 ccatcaattc gggcaaatgc cgcatcagtg cgatattgtg ttgcaggttc ttgccgaagc
    16441 cgaaacccgg gtcgagcgta atccgttgcg gcgcgatgcc tgccgcgaca caggtttctg
    16501 accgtgtttt cagataacgt gccacttcgc cgaccacatc ctgatatttc ggattgtcct
    16561 gcatggtttc gggcaagccc cgcatatgca tcaggcaaat gcccgtgtcc gcctggcgcg
    16621 ccagcaattc gaccgcacct tcgtcggtca acgccgccac atcattgata atatcgatgc
    16681 cgccgagtgc caacgccttt tccataacca ccgtgcggcg cgtgtccaaa ctgacgggaa
    16741 caccccaccc cgccgcttcc gccaatacag gctcaacccg cgcccattct tcttcttcgg
    16801 gcggaacgaa atccgcaccc ggccgcgtcg attcgccgcc gatgtcgaga atgtccgcac
    16861 cctctttcaa aagccgttcg gcatgcgcca aagctgtttg ggcgttttgc gaatacgcgc
    16921 cgccgtcgga aaaagaatcg ggcgtgagat tcacgatgcc catgattttc ggtttgtcca
    16981 aaccgatttc aaaccgtcct gcccgccaaa cgtgtcgtgc catctgaact cctcccgaaa
    17041 taaaaaacag attatatgcc gtctgaaacc gtcttgtgcg cttcggacgg caccgctatt
    17101 cgggcggcag acggcatgtt gtcccaatgt ctgcgccgcc tttgaatctg ccggcatgcc
    17161 tgctatccgc ccgccttttc aaaacaggtt ccgacgattc cgcacgcgcc tgccgccttt
    17221 gccaagccgt acaggatttc ctgcggcata tcgcggctcc ataatcccgt aatattcgca
    17281 atcacgggca gatggctgat ttggcggact ttcacgatgg attcgacatc caaacggtag
    17341 ggatggcctt tggtatggcc caacacgccc gactcgccca gaatcaggtg gcggttgccg
    17401 cgcgagacga catattctgc ggcattcaac caatcttcgg cagaatggtg cttgtcttta
    17461 cacagaacca agggaatgtt caggcttccg gcttcattca atacggcaag atcggacatc
    17521 agcccgccgc ccaaatacag gatgtccgct cccgcattca aagccgcttc gacatggcgg
    17581 acgttgcgaa cgcgcaccaa tacgggtttc cctgcgccat gcgccgatgc ggcctgttcc
    17641 gccaaccgtc tgcaccgtcc ccgcccttca tccgcactta aagtgtcgta taagtttgcc
    17701 gaagtgaaaa acggatccag aaacactgca tccgcattgc tccatactga cggttctgca
    17761 gcgatacgga cggtttcccc gccgccgaaa gccacgcctt tggcggcaac gcggctgtct
    17821 tccgcccgat tttcccgact gacggttttc catgtgtcca aaatgcggac ggctttctcg
    17881 acctccggca gcgtctgtac ctccctgacg ctcaaaaccc tatcgtcgcc gattgcgccg
    17941 atgacggtac gctcgtcgcc gtgagaaatg tgttctcgca gacctctgct gcggatgaag
    18001 gcgacaacgc cggcaatgtc cgcttcggcg gcacgcctgc tcatgacaat aatcatattt
    18061 cctcctgaca caagagacgg cccgcccaaa ataggatttt tgcaagccgt gttatactgt
    18121 ggcgtgtttt acagattgtt cgggctatgg atttattatc ggttttccac aaataccgtc
    18181 tgaaatatgc ggtggcggtg ctgacgatgc tgcttttggc ggcagtcggg ctgcacgctt
    18241 ccgtatatcg caccttcacg cccgaaaaca tccgcagccg cctccaacaa agcattgccc
    18301 atacccaccg gaaaatctcg tttgatgcgg atatacggcg caggcttctg ccccgcccga
    18361 ccgtcatcct gaaaaacctg accattaccg aacccgacgg cggccgggtc gccgtttccg
    18421 tcaaagaaac caaaatcgga ttgagctgga aaaacctgtg gtcggatcgg atacaggttg
    18481 aaaaatgggt ggtttcgggt gcggatcttg ccctgacgcg cgacagaaac ggcgcttgga
    18541 acatccaaga cctgttcgac ggcgcgaaac actccgcctc agtcaaccgc attatcgtcg
    18601 aaaacagcac cgtccgcctc aatttcctgc agcaacagct tatcctgaag gaaatcagcc
    18661 tcaacctgca atcccccgat tcgtcggggc agcagtttga aagttcgggc atactggttt
    18721 ggagaaagct gtccgtcccg tggaaaagca gggggctgtt cctttcagac ggcatcggca
    18781 cgcccgaaat ctcaccgttc cattttgaag cttccacttc gctggacgga cacggcatca
    18841 ccatttccac caccggcagc ccttctgtcc gcttcaacgc cggcggagcg gatgccgccg
    18901 gcctcggcct gcgtgcagac acttccttcc gcaacctcca cctgaccgcg caaatccccg
    18961 cactggcact caaaaacaac agcatcaaaa ccggcacggt caacggcacg tttaccgccg
    19021 gcggcgaata tgcccgatgg gacggttcgt tcaaactcga caaagccaac ctgcactccg
    19081 gcatcgccaa catcggcaac gccgaaatct ccggcagctt caaaacaccg cgccttcaaa
    19141 ccaatttctc cctcggctcg ccgttggttt ggagtcggga caacgggctg gacgccccgc
    19201 gcctgcacat atcgaccctt caggataccg tcgaccgcct gccgcaaccc cgtttcatca
    19261 gccggctcga cggttcgctg tccataccga atctgcaaaa ttggaatgcc gaattaaacg
    19321 gcacattcga ccgccaaccc gttgccgcaa aattcaaata tacgcgggaa ggcgcaccgc
    19381 gcctggaagc cgccgccgcg ctgcaaaaat taaacctcgc cccctatctt gacgaatttc
    19441 ggcaacaaaa cggcaaaata ttccccgaca tcctcggcag gctgtccggc aacgtcgagg
    19501 cacacctcaa aatcggcagc atccaactcc ccggcttgca actggacgat atggaaacct
    19561 acctccacgc cgacaaagac catatcgcgc tcagccgttt caagtcaggg ctttacggcg
    19621 gccataccga aggcggcatc agcatcgcca acacccgtcc cgccacttac cgcctgcaac
    19681 agaatgcaag caacatccaa atccaaccgc tgctgcaaga cctgttcggc ttccacagct
    19741 tcagcggcaa cggcgatgcg gtcatcgacc tgaccgcaag cggcgaaaac cgcaaacagc
    19801 ttatccgctc gctgcaaggc agcctgtcgc tgaatatttc caacggcgcg tggcacggca
    19861 tcgatatgga cagcatttta aaaaacggcc tttccggaaa aatctcgggc agcacaccct
    19921 tctaccgatt cacgctcaac agcgaaattt cagacggcat cagccgccac atcgataccg
    19981 aactcttctc cgacagcctc tatgttacca gcaacggcta taccaatctg gatacgcagg
    20041 aattgtctga agatgtcctt atccgcaacg ccgtccatcc gaaaaacaaa ccgattcccc
    20101 tgaaaatcac cggtacggtg gacaagccgt ccattaccgt cgattacggc aggctgaccg
    20161 gcggcatcaa ttcgcgcaaa gagaaacaga aaatcctcga agacaccctg ctggaacaat
    20221 ggcagtggct caaacctaaa gaaccgtaaa taccctgcgt acaaaaatgc cgtctgaaac
    20281 acattaatct tcagacggca tgaccgtaaa acctacaacc ccaatttccc ccaaatccca
    20341 tcaattttaa ccgtaaccgc agggtctttt ttgattaccc gtccccattc gcggtcggtt
    20401 tctcccggcc atttgctggt cgcatccaaa cccattttgc cgccgagtcc gctgacgggg
    20461 ctggcgaagt cgaggtagtc gatgggcgtg ttttccacca aaacggtgtc gcggacgggg
    20521 tccatgcgcg tggttaccgc ccagatgact tctttccagt cgcgcacgtt tacatcgtca
    20581 tccaccacga tgatgaattt ggtgtacata aactggcgca ggaacgacca gcagcccgtc
    20641 atcacgcgct tggcgtgtcc ggcgtactgt tttttcatgc tgaccaccgc catgcggtag
    20701 gaacagcctt cgggcggcag gtagaaatcg gtgatttcgg agaactgctt ttgcaaaagc
    20761 ggtacgaaca cttcgttcaa cgccacgccc aaaacggcag gttcgtcggg cggtttgcct
    20821 gtgtaggtag agtggtaaat cgggttttcg cgcatggtga tgcgttcgac cgtaaacaca
    20881 gggaaatggc cctgctcgtt gtaatagccc gtgtggtcgc cgtatgggcc ttccaacgcg
    20941 gtttcgtttg gatgaatcac gccttccaat acaatttcag cacgggcagg cacttgcaaa
    21001 tcgctgccga tacatttcac cagctccgtc cgcgaaccgc gcagcagtcc ggcaaactgg
    21061 tattcgctca aagtatcggg aacgggcgtt accgcgccca aaatggtgga ggggtcgcaa
    21121 ccgagtacga cggcgacggg atacggcgta tcgggattga gtttgcggaa ttcctgataa
    21181 tccaacgcgc cgccgcgatg cgacagccag cgcataacca gcttgttttt gccgatgagt
    21241 tgttggcggt aaatgccgag attttggcgt tttttgtgcg gcccgcgcgt gacggtcaag
    21301 ccccacgtta ccagcggcgc aacgtcttcc ggccagcaat gttgaatcgg aagctgatac
    21361 aaatcaacgt cttctccttc ccacacgatt tcctgacacg gcgcgttttt caccacgttc
    21421 ggcgccatgc tccaaatatc tttcaacagc ggcagtttgg aaaacgcgtc tttgatgcct
    21481 ttgggcggtt cgggttcttt caaatacgcc agcgtctgcc cgatttcgcg cagcttggac
    21541 acgctgtccg cgcccatgcc catcgccaca cgttcggggg tgccgaacag gttcgccaac
    21601 acgggataat catagcgcgt accgtcgggc ttaaccgggt tttcaaacaa caacgccggc
    21661 ccttcggcgc gcaacacgcg gtcggcaatt tcggtcattt ccaaatgcgg ggaaacgggg
    21721 tgggcgacgc gcttgagctt gccctgctgc tcgagcatag cgatgaagtc gcgcaggtct
    21781 ttgtatttca tgtttatcct ttttgtcctt ttatcctgaa caatccgatt cggataccgc
    21841 ccctatcctt gcctgcgctt cggcatatcc catgccgtga taaaagtcgc gtaccggcgg
    21901 atgtccgccg ctttgatgaa gttgcagcaa agggcgttgg ttgtcggact gggtaacgac
    21961 attgcagtgc agaccgaagg tgtcggtttc ataagggggc agccgattgc aaatcatacc
    22021 gaaataaacg gcgttttcag ggttgtcgta aaagcggctt tgatagtcgt taaaactctt
    22081 ttcgctgacg gacacccaca cgccgtattc cagcgtttcc tgatgcccga taatcggaat
    22141 aggcagcgcc gcgcggataa agcggtcggt ttggtcggaa tagcggatga tgcagaaatc
    22201 agaatcgcat tccgcttgat aagcaatgcg ttcttcttca ctgagttgat tatagggata
    22261 gggggcggta aagccggttg cgggcatatc ttcatgtttt tcgccgcagg aagtgcaagt
    22321 gtgcataagg tttcagactt tcaacacaag tttgcggtaa agccattcgc cggcaaacag
    22381 catccccatc aatacatagg caatcacgcc tgtataaacc gcccaccaat catatcgccc
    22441 caaccgggcc aacaaagcgg caagcgtccc gttggcaata aagaatacgc accaaacctg
    22501 cgttacccgg cgggtatagc gcacggcttt ttcaggcagg tcgggctgtt gcagccgcgc
    22561 aagtttttca atcaccgtct gcccggcaaa caggctgctg ccgaacaccg ccaacatcat
    22621 caaattaacg aggacaggat accaatacat tgaatcacgc cgcccgaaca ccaatactgc
    22681 ggcaaaaaac agtgtaatat agtggattaa caaaaatcag gacaaggcgg cgaagccgca
    22741 gacagtacaa atagtacggc aaggcgaggc aacgccgtac cggtttaaat ttaatccact
    22801 ataccacaaa gcgggatagg caatgcttaa tacggtcaaa aaaatatgtc cgaaaaaacc
    22861 gggtttcatt ttgaatccgc acaaacgttt tcagacggca tccgataaaa acatgccgtc
    22921 tgaaaaataa ttagaaatac ccgattagcc ggcctgaatc ttcaataccg cctgtaccac
    22981 gtcgttgacg gtgcggacat tgcggaaatc ttcagcctgc agcttgcggg ctgccacaac
    23041 cattcacgat tgcgatatac ccggcggcat tcccgactgc cgttcagcag aaaacgcacc
    23101 cattccaaac cgctccaata tgcctcgggc agcataccgg cttcagacgg catatcgtcc
    23161 gaagcagaca aagtcaggtg taacgcgtcc ctttggtcag aaccatcgcc aaagcataag
    23221 caaacggcgc gcgcgttgcc gatacggcat atccttccgg cagcggatcg tccgccgcca
    23281 aaaccaaaac cgacccgcat ccctcttcca acagggacgc cgcttccgcc aatgccgttt
    23341 ccacaccgtc ggcacacacg gccaatgccg tctgctcgct catatcccgc ctcaatatcg
    23401 accattgccc cgccgtcgca ttgtgcaccg acaaaccgaa cgaagtcggc gacacggtat
    23461 gcgatttcaa cagttccaac cacaaatcga aactacgcgc catttccccg tcgtgcgagg
    23521 cataaactac cggactgccg ggatgggcgg aggcaatgtc ccaagccgcg tcgcatacca
    23581 aacgcgccgc cttactcaaa cggcggcgcc gcatagcggg caggaacggc aattcccgcc
    23641 tgacatcggg caaaccgtcg gcaaaatccg gacattccgc ccactgcctc cactgggcca
    23701 tatcgcgcat tttgccgccc gaaacccgcc agtcggcgat gtcgaagtgg aaccggcaaa
    23761 taatcggcgg catagtttct ttcaaaaatt tacactgtgc cgcattctaa ccaaagccca
    23821 tccccctgac aatgccgaaa ttcaaacgca tccacgccat tttttccgac aacgccgccc
    23881 ctcggcaaac cgcccgaatt agcctgaatt tacatttatc attgataatg cccgattacc
    23941 gacaccgcct ccctgctgcc gcacagcggg cgtatggttc tgatagaccg cattacccga
    24001 tacggcgatg attttgtcga agcaggggcg caggtaaaac ccgaccacat cctgctggcc
    24061 ggcggcatac tgccctatac ggcatttatc gaactgatgg cgcaggctgt cggcgcatat
    24121 gccggcatcc aagcccgaaa aaacgcacaa ccggtccggc tcggcttcct gcccggcacg
    24181 cgcaaacttg aaatcttcgc ccaatccgtc cctgtcggaa cacatttgcc ggcaacggcg
    24241 catatgtcta tccaagatgc cggggtatgg gcgtgtccga ctgcgaactg cgttggacgg
    24301 atgcgccgga aacttcgtcc ggaacactcc cttcagacgg cattttggcg cgcgcctcac
    24361 tcaacgtgta cagccccgaa caccctgtcg aaacaactta atgccgtctg aacagacacg
    24421 cacatacgga gaacaaccat gactgaaacc gtcctgatta ccggctcaaa cagaggcata
    24481 ggcaaagccg ccgcactcgg ttttggcgga cgacggcttt gatatcgccg tccactgccg
    24541 cagccgccgc gacgaagccg aagccgtggc ggaagaaatc cgcgctttgg gcagaaatgc
    24601 gcgcgtgttg cagtttgacg tgtccgaccg cgaagcctgc cgcgagattc tgaccgccga
    24661 catcgaagca aacggcacgt attacggcgt ggtgttgaac gccggcctga cgcgcgacaa
    24721 tgccttcccc gcgttttcag acgacgattg ggatgtggtg ccgcggacta atttggacgg
    24781 tttttacaat gtactgcatc cgctggttat gccgatgata cgccgccgca aagccggacg
    24841 gattgtgtgt atggcatcgg tgtccggcct gacgggcaac cgggggcagg tcaattacag
    24901 cgcgtcaaaa gcgggcatta tcggcgcggc aaaagccttg gcggtcgaac cggcgaaacg
    24961 caaaatcacc gtcaactgcg tggcgccggg tttgatcgat acggagattg tcgatgaaaa
    25021 cgtacccgtc ggagaaattt tgaaagccgt ccccgccgcg cgtatgggac tgccggaaga
    25081 agtggcgcac gcagtgcact tcctgatgga tgaaaaagcg gcgtatatca cgcgccaggt
    25141 gattgcggtg aacggaggtt tgtgttgaat accagaaggg tcgcagtaac gggcataggc
    25201 ggcattaccg ccttcggccg ggattggcaa agcatacagg cagcattcaa agccgaaaaa
    25261 aacgccgtca aatatatgga ttggcgcgaa cgtttccccg aattggaagc gcaactgggt
    25321 gcgccgattg aggattacgc gccgccgaaa cattggacgc gcaagcggct cagaagtatg
    25381 gggcgcgtgt cgtacctgtg cgtcgatgcg gcggagcagg cgctggcgga tgccggtttg
    25441 ctcggagacg aaagcattac cgacggacgg atgggcgttg cctgcggctc ttccggcggc
    25501 ggcaccaaag acatcggaga tgtgggcgaa ttgttgctga ccggcacgtc gcgcaacttc
    25561 ggcgccaata cctatgtgcg tatgatgccg cacaccgccg acgccaatat cggcatcttt
    25621 ttcggattaa caaggacgca tcatcccgac atcgagcgcg tgttcttcgg gcagccaagg
    25681 cataggttat gcttacgaga ccatcaaata cggtctgacc gatatgatgc tggcgggcgg
    25741 cggcgaagaa ttttgcccgt ccgaagtgta tgttttcgac tcgctctatg ccgccagccg
    25801 ccgcaacggc gaaccggaag agaccccgcg tccatacgac gcgaaccgcg acgggctggt
    25861 catcggagaa aaggcgcggg gattttcgta ttggaagaat tggaacacgc caaacggcgc
    25921 ggtgcgaaaa tttacgccga actcgtcggc tacggcgcca acagcgacgg catcaatatc
    25981 ggcgaaggcg cagccgtatt catcatgacg cgcgatgcgg atttttccgg cggtatgcag
    26041 cttctgggtt acggcgcaag cagcgatgcc taccatattt ccacgccccg ccccgacgca
    26101 aaggcgcaat cctcgccttt cagacggcat tgcagcacgc cggccttgcg cccgaagaca
    26161 tcggccggat taatctgcac ggcaccggga cgcaccacaa cgacagtatg gaaagccgcg
    26221 ccgttgcagc ggttttcggc aacaatacgc cctgcacgtc cgccaagccg caaaccggac
    26281 acacgctggg cgcggcgggc gcaatcgaag ccgcgttcgc gtggggcatt gccgaccggc
    26341 aaagcaatcc cgaagggaaa cttccgcccc ggctttggga cgggcagaac gaccccgacc
    26401 tgcccgccat taacctgacc ggcggcggct gggaaaccga aaaacgcatt gccgccagct
    26461 cgtcgtttgc cttcggggga agcaactgcg tcttaatcat cggatgaaat aagtttgtca
    26521 atcccaccgc tatgctatac aatacgcgcc tactcttgac gggtctgtag ctcaggggtt
    26581 agagcagggg actcataatc ccttggtcgt gggttcgaac cccaccggac ccaccaattc
    26641 ccaagcccgg acgtatgttt gggctttttt gccgccctgt gaaaccaaaa tgctttgaga
    26701 aaccttgata atgaaaaaag tcagcgtatt gattgttgcc aaaaacgaag caaaccacat
    26761 tcgggaatgt attgaaagtt gccgtttcga taaagaagtt atcgttatcg acgaccatag
    26821 cgccgacaac actgccgaaa ttgccgaggg tttgggtgca aaagtcttca gacggcattt
    26881 gaacggggat ttcggagcgc aaaaaacatt tgccatcgaa caggcaggcg gagaatgggt
    26941 tttcctgatt gatgcagacg aacgctgcac gccggaacta tctgatgaaa tctcaaaaat
    27001 tgtccgaacc ggcgattatg ccgcctattt tgtcgaacgc cgcaaccttt tccccaacca
    27061 tcccgccaca cacggcgcga tgcgtcccga cagcgtatgc cgtctgatgc cgaaaaaagg
    27121 cggttcggtg caaggcaaag tacacgaaac cgtacagacc ccctaccccg aacgccgtct
    27181 gaaacatttt atgtaccatt acacgtacga caactgggaa caatatttca acaagttcaa
    27241 caaatatact tccatctcgg ccgagaaata ccgggagcag ggaaagcccg tgagtttcgt
    27301 tagggacatt atcctccgtc cgatttgggg ttttttcaaa atttatatcc tgaacaaagg
    27361 gtttcttgat ggaaaaatgg gttggattat gtccgtcaac cacagctatt acacgatgat
    27421 taaatatgtc aaactatatt atctgtacaa atccggcgga aaattttaaa tggaaaaaga
    27481 attcaggata ttaaatatcg tatcggccaa gatttggggt gggggcgaac aatatgtcta
    27541 tgatgtttca aaagcattgg gacttcgggg ctgcaccatg tttaccgccg tcaataaaaa
    27601 tgatgaattg atgcgcagac gcttttccga agtttcttcc gttttcacaa cgcgccttca
    27661 cacgttcaac gggctgtttt cgctctacgc gcttacccgc tttatccggg aaaaccacat
    27721 ttcccacctg atgatacaca ccggcaaaat tgccgcctta tccgtacttt tgaaaaaact
    27781 gaccggggtg cgcctgatat ttgtcaaaca taatgtcgtc gccaacaaaa ccgattttta
    27841 tcaccgcctg atacagaaaa acaccgaccg ctttatttgc gtttcccgtc tggtttacga
    27901 tgtgcaaacc gccgacaatc cctttaaaga aaaataccgg attgttcata acggtatcga
    27961 taccggccgg ttccccccgt ctcaagaaaa acccgacagc cgttttttta ccgtcgccta
    28021 cgccggcagg atcagtccgg aaaaaggatt ggaaaacctg attgaagcct gtgtgatact
    28081 gcatcggaaa tatcctcaaa tcaggctcaa attggcaggg cacggacatc cggattatat
    28141 gtgccgcctg aagcggggcg tatctgcttc gggagcagaa ccatttgttt cttttgaagg
    28201 gtttaccgaa aaacttgctt cgttttaccg ccaaagcgat gtcgtggttt tgcccagcct
    28261 cgtcccggag gcattcggtt tgtcattatg cgaggcgatg tactgccgaa cggcggtgat
    28321 ttccaatact ttgggtgcgc aaaaggaaat tatcgaacat catcaatcgg ggattctgct
    28381 ggacaggctt acgcctgaat ctttggcgga cgaaatcgaa cgcctcgtct taaaccccga
    28441 agcaaaaaac gcactggcaa cggcaggaca tcaatgcgtc gccgcccgtt tcaccatcaa
    28501 ccataccgcc gacaaattat tggatgcaat ataagccgct ttcagacggc ctatgccgtc
    28561 tgaaagcctt tgatgcaaca aaccgccaaa ttatattcgt tcattggaaa gaaacacccc
    28621 gaattcatcc ttcaaaataa gaaaatccca atatcccccg atatcgcgca gcctattggc
    28681 aaagttttgc agcgtcttcc ccggcttgtg ccgccgcgtc aagtgctttg ttacaatgca
    28741 ctaccttcac atttcttaat aaattttatg agtaaccata cttcttggtc gtccaaaatc
    28801 ggtttcgtac ttgctgcggc aggttcggcc atcggtttgg gcgcgatttg gaaatttcct
    28861 tatacggcgg gcaccaacgg cggcgcggtg tttttcctgc tgtttttgat atttaccgtc
    28921 ttggtcgccc tgcccgtcca gcttgccgaa ttttatatcg ggcgcacggg cggtaaaaat
    28981 gccgtcgatt ccttcagggt tctgcgtccg ggtacgcaat ggctttgggt cgggcgcatg
    29041 ggcgttgccg cctgctttat tttgctgtcg ttttacagcg tggtcggcgg atgggtattg
    29101 aattatgtcg tccacagttt tacaggggca atccatgcgg gagcggactt tgaagccctg
    29161 ttcggcacaa cgatttccaa tccggcaggt tcgctgtcct atcaggcact gtttatgctg
    29221 attacggttt gggtggtcaa aggcggcatt tcagacggca ttgaaaaggc aaaccgttat
    29281 ctgatgccgg ggctgtttat cctctttatt gcgctggctg tccgttcgct gacgctgccg
    29341 gatgcaatgg agggcgtgtc tttcctgctc aaacccaatt ggtcgtactt taaagccgat
    29401 acgatgatta cggctttggg ccaggcgttt tttgccctga gcatcggcgt ttccgccatg
    29461 attacctatg cttcgtattt gggaaaagat caggatatgt tccgttccgg ccatacgatt
    29521 atgtggatga acctcttggt ttccctgctt gccggcctgg tgattttccc ggcggtattc
    29581 gccttcggtt ttgaaccgaa ccaggggccg ggattgattt ttatcgtatt gcccgcagtg
    29641 ttcatgaaaa tgccgttcgg tacggtttta tttgcagtat tcatgcttct ggtcgttttc
    29701 gccacgctga cttcggcatt ttcgatgttg gaaacggtca ttgcctcaac catccgccaa
    29761 gacgagcgca aacgcaaaaa acatacttgg cttatcggca cggctatttt cattgtcggc
    29821 atcccgtccg cgctgtcttt cggcgcatgg ggtgagttta aggttttcgg caaaaccatt
    29881 tttgatttgt gggactatgt tatttccgcc gtcattatgc cgattggtgc tttgagtgtt
    29941 tccatcttta ccgcctggat tcaggacaag cagtctgtgt taaaagatgc cggcgcgggc
    30001 agcaccgtac cacgggcagt gctgctgctg tggctgaata ccttgcgcta ccttgccccg
    30061 attgccatta ttattgtttt cgtcaatttt ttaggcatcc tttaaacata ccgcccgacg
    30121 gcaaaatgcc gtctgaaagc ctttcagacg gcattttgct tcagattcaa cctatttcgt
    30181 tcaaagtata gtggattaaa tttaaatcag gacaaggcga cgaagtcgca gacagtacag
    30241 atagtacggc aaggcgaggc aacgccgtac cggtttttgt taatccacta tattgtccgg
    30301 catggccagc ctaactttaa gccgcctgct ttcggcaacc gaccgtatca gcggcaaatc
    30361 catcacggaa aacgccgtcg acaaggcatc ggcaaccgcc gcatcatccg ccattacgct
    30421 catgctttta taacgcggcg tactcacgcc ggttcgcgga tcgaacaaat gggtaagcct
    30481 gcccgctttg tccatcaccg tcccatagcc gcccgaagtg gcaaacgcct tgtctttcac
    30541 cgtgatattt gccaacacgc cttcttcgtc gtccggattg cggatgccga cattccacat
    30601 ccgcctgccg tttgtatcaa accccctgat ttcgcccata tcgaccagcg cggcaggcac
    30661 tccgtttgct ttaagcagcg caacaacctt gtccgtgata tagccttgcg cgatgccgtt
    30721 caaggacaaa cccataccct tttcggcaaa acggatttca cggtcgtcaa acgagacttt
    30781 gccgaagccg acccgtttca gggtttcctt cacgctattc tccggcggcg tttccgcatc
    30841 gggacgggcg gcaaaataat cggcatacag tttccacaac gcctgaaccg tcggatcgaa
    30901 cgcgccttcc gtcagcgcgt gaatatcgcg gcacaggctc aacagttcca aaaaatccgc
    30961 cggaggcgaa gtcagataac cgtcgcggtt cagacggctg atcaggctgt cttcacggta
    31021 aaggctgaac attttttcca aacgcgccac ttccgccaaa accttattga ccaaatccgc
    31081 cgcctgtctg tcgtccacgc cgaacaggcg cagctccgcg ccggaaccca gcgcgacacc
    31141 tttccagaaa aacacatttt catcgcgttt tttatccatg ttgcgttttt cgccgccggc
    31201 ggcaaaagga ttcggcaaaa aagaaaccgc cgcaccggcc acagcaacgg cggcagccgt
    31261 cagaaaacgt ctgcgcccga aatgcctgcc cataccgcct cctaaaccga cactaccgcc
    31321 ttgatgtgcg gatgcggatc gtagccttcc aactcgaaat cttcaaactt gaaggcaaac
    31381 aaatccttga cttcaggatt gattttcatc acaggcaagg cgcgcggttc gcgttccaac
    31441 tgcaatgcgg cctgttcgaa atggttacgg tacaaatgcg cgtcgccaaa cgtatggaca
    31501 aactcgcccg cctccagccc gcacacttgc gccatcatca tcgtcaacag cgcgtagctg
    31561 gcaatattaa acggcacacc aaggaaaata tctgcactac gctggtaaag ctggcaggac
    31621 agtttgccgt cggcaacgta aaactgaaac agcgcgtggc agggcggcaa ggccatctca
    31681 tcgaccaaag ccggattcca cgccgacaca atcaggcggc gcgagtcggg gtttttctta
    31741 atttgttcca ccacattggc gatttggtcg atatgcctgc cgtcgggcgc gggccagctg
    31801 cgccattggt agccgtaaac cgggcccaag tcgccgtttt cgtccgccca ctcgtcccaa
    31861 atggaaacat tgttatcctt caggtatttg atattggtat cgcctttgag gaaccaaagc
    31921 agctcgtgga ttatcgagcg caaatgcagc tttttggtcg tcagcagcgg aaaacctttg
    31981 cccaagtcaa aacgcatctg ataaccgaat acggagcgcg tacccgtacc ggtgcggtct
    32041 gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt ccaaataggc cttcatcatc
    32101 atctttcatc aaattaaacg gcacatattg taacatttcc ggccaacacc cgagtccgga
    32161 caaacgcgca ggccgccccg ccggacgctc aaaacgcgga tgaaggcagg caggattgtt
    32221 ggcaatttca gccctttttc acagcaaaag caggcgtgaa aacaaaatta tcttgattgg
    32281 aatcaaaaaa tctagtttaa ctacttagaa taaaattcca ataatatttt ttatttgaga
    32341 aattaattta tgattattgg ttgattatcg gcaataaaca tcatatatcg aaaatatggg
    32401 aaaaataatg tcaacaattt gtgcaaaatt aggcttggca tcagaaaaaa ataggtttat
    32461 attgccaact acaaatttgt tttcccatta gtacaatatc aaccaaaagg agtatccgaa
    32521 tgactgacct gaacaccctg tttgccaacc tcaaacaacg caaccccaat caggagccgt
    32581 tccatcaggc ggttgaagaa gtcttcatga gccttgatcc gtttttggca aaaaatccga
    32641 aatacaccca gcaaagcctg ctggaacgca tcgtcgaacc cgaacgcgtc gtgatgttcc
    32701 gcgtaacctg gcaggacgac aaaggacaag tccaagtcaa ccgcggctac cgcgtgcaaa
    32761 tgagttccgc catcggccct tacaaaggcg gcctgcgctt ccacccgacc gtcgatttgg
    32821 gcgtgttgaa attcctcgct tttgaacaag tgttcaaaaa cgccttgacc accctgccta
    32881 tgggcggcgg caaaggcggt tccgacttcg accccaaagg caaatccgat gccgaagtaa
    32941 tgcgcttctg ccaagccttt atgaccgaac tctaccgcca catcggcgcg gacaccgatg
    33001 ttccggccgg cgacatcggc gtaggcggac gcgaaatcgg ctacctgttc ggacaataca
    33061 aaaaaatccg caatgaattc acttccgtcc tgaccggcaa aggtttggaa tggggtggca
    33121 gcctcatccg ccccgaggcg accggctacg gctgcgtcta tttcgcccaa gcgatgctgc
    33181 aaacccgcaa cgatagtttt gaaggcaaac gcgttctgat ttccggttcc ggcaacgtgg
    33241 cgcaatacgc cgctgaaaaa gccatccaac tgggtgcgaa agtgctgacc gtctccgact
    33301 ccgacggctt cgtcctcttc cccgacatcg gtatgaccga agcgcaactc gccgccttga
    33361 tcgaattgaa agaagtccgc cgcgaacgcg ttgccaccta cgccaaagag caaggtttgc
    33421 aatactttga aaaccaaaaa ccgtggggcg ttgccgccga aatcgccctg ccctgcgcga
    33481 cccagaacga attggacgaa gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg
    33541 ttgccgaagg cgcgaatatg ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg
    33601 gcatcctcta cgctccgggc aaagcctcca acgccggcgg cgtggcaact tcgggcttgg
    33661 aaatgagcca aaacgccatc cgcctgtctt ggacgcgcga agaagtggac agccgactgt
    33721 tcggcatcat gcaaagcatc cacgagtcct gcctgaaata cggcaaagtc ggcgacaaag
    33781 tgaactacgt caacggtgcg aacatcgccg gcttcgtcaa agttgccgat gcgatgctgg
    33841 cgcaaggctt ctaagcaaac gcctccgccc cgcaaacaaa atgccgtccg aaccgcaaat
    33901 gctgttcaga cggcatttca ttatccgccc gttcgaatcg ggtaatacta tagtgaatta
    33961 aatttaaatc aggacaaggc ggcgagccgc agacagtaca aatagtacgg caaggcgagg
    34021 caacgccgta ctggtttaaa tttagttcac tataccgata catccgaata tgccagaccg
    34081 tctgaacggt attcatacct cccaatcctg cacgcgcttc aaatcgtttt gcgccaaagt
    34141 atccgcgtgg cggttacggc tctgatattc cctgtctttc aatatgctgc tcgccacata
    34201 attcaaatga gcctttgccg cctccgaagc ctcgcccggc cggcggttcg atatcgcctc
    34261 atacagcacg cggtgctgcg ccatcagttt cggacgcgga tcttcttcct gattcagata
    34321 aataaggctg ctgcgcgtct gccggtacag cattttcaac aaaccgcccg acaaatggct
    34381 gaacaacaaa ttgtgcgccg catccgcaat cgtctgatgg aagctgacat cggcttcgcc
    34441 ctgatgttcc agattgccgc ttccgcacgc ttcctcaaac ttttcaagcc aaaaccgaat
    34501 ccgcttcaaa tcggcatccg tccggcgttc tgccgccaat gccgccatac agccctcgat
    34561 gtggcagctg aaatcaaaaa catcctgttc ccaattggaa tgtttgccca aaagctcctg
    34621 ccaactttgc aaaaaatcct gctgcggctt gaccgaaaca taataaccat cgccctgcct
    34681 cgcttccaaa atctgacggg caaccaaaat attcaatgcc gaccggaccg acggacgcga
    34741 aacgccgaac tcttccgcca aaacgcgttc gggcggaatc ttgccccctt ccgcgtaaac
    34801 cccttccacg atgcggcctt ccaataccga caatacctga tcgctgattt tctgaggcct
    34861 taccagtttc atcactcctc ctttataaag attccctgca gaacccttcc gaaatacatc
    34921 aaacatcaaa ttggactggc caatcagggc ggattctaat gacacgccgt tcccgccgtc
    34981 aacggcactt acctcgcacc gcccccgaaa cacaggaaaa aactacacca aactacaatt
    35041 tttgttcatg caaatatttg ttttaacggg atttaaatga aagctccgat tcaaatctgc
    35101 cgaaccgccc aaaaatatat tgacctgaat attaaagttt cgtaaagtaa tgcaacgttg
    35161 ctttaattgg tttgaccact attgccgacg attagaaaaa tattttcgga gatgttcaat
    35221 tatggaaact tgggttcaaa actacacggc aatcggcggc agcctgtatc tgactgccgc
    35281 cgccgcactc ttacccatcg tctttttctt tgccgcgctg accgtcctga agctgaaagg
    35341 ctatcaggcg gggctttata cgctgctgat tgcgcttgcc gttgccgtat tcggcttcgg
    35401 gatgccgacg ggtatggcgg tttcttccct gtcgccgcag ccggattgac ccaacgcccc
    35461 atacgctaca ctggacttga ccgcgcatta caaaatcggc aaatccaccc gcatcggttt
    35521 ggactttgaa aacgtcttta acaaacgctg ccgcacgatg cccgacattc acgtttacgg
    35581 cacgccgcgc agcctgaccg caaccgtcaa acatatagtg gattaacaaa aatcaggaca
    35641 aggcggcggg ccgcagacag tacaaatagt acggaaccgg ttcgcccggt gcttggacac
    35701 cttagggaat cgttctcttt gagctaaggc ggggcaacgc cgtactggtt tttgttaatc
    35761 cactataaag aaagaaatgc cgtctgaaac cttatcgttt cagacggcat ttgtttgccc
    35821 gggcggttat ttgccggttt gggtatcccg tttcaatccg ccgccgagcg ccttgtacaa
    35881 atcggcaagg ttttcggcgc gggtcagttg tgccgacaaa gccgcacctt ccgccgaata
    35941 gctgctgcgt tccgcatcga gcaaatcgag cgcgccggat acgccgtgtt tgtaacgcag
    36001 tccgaccaag cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc
    36061 tttatccagc tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaagg cggattggac
    36121 ggcggattca taggcaacaa tttgtgcctg ttggcgcagt tttgccacat caaggttcgc
    36181 cttgttcgtt ccccaagtaa aaatcggcag ggtaatagac ggcgcgaacg cccaaacgcc
    36241 cgtgccgctt ttgaacagcc cgcccaattc gacagaaccc gtaccgacgc ttccggtcag
    36301 gcggatggac gggaaaaagg cggcgcgcgc cgcaccgata ttggcgtttg cctgtttgag
    36361 cgcgtgttcg gcggcgcgga tgtcgggacg gtcgagcaat acttcggaac tcaaaccggc
    36421 aggcagtttt tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcttcggg
    36481 tatcggacgg ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc
    36541 atgggcataa tcggctttgg cagattcaat caaggcttcc tgctggcgca gggcgacggc
    36601 ggaaatcacg cctgccttgt accgcaattc ggacagcttg taggtttcct cgcgcgtttt
    36661 caagacacgc tgcgccaaag acatcgcttc ttcggcataa cgctcgttga aataggcttt
    36721 ggcaacggtg gcaatcagac tcaaatgtgc cgcatcgcgg ttggcaacgc tggcaaaata
    36781 gccttgcagt gctgcttcgc tgctgctgcg cacgcgcccg aacagatcga gttcgtaaga
    36841 tgccgcaccc agtccgacat tgtagctgct gctgacattg ccgccgctca agctgccttg
    36901 gcgcgagccg ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct cgatcatgta
    36961 ttgtttgcgg tagatttcgc tgttcaatac ggctgtacgc aaactggtat tgcgctcgag
    37021 tgcgatgtcg atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacccaaatc
    37081 aaccgcgcgg atggaagaaa ccgatgtgtc gttttggaag gtttccgcaa cttcgacttt
    37141 gggctgctcg tattgaggaa tcatggtgca ggcagacaat gcaaaggctg ctgcaacaga
    37201 ggtcaaggta gttttcaatg tagtattcat aaaaaagtcc tgatgccgtc tgaaaaccgg
    37261 cggttctcag acggtatttt gcttaatatt gtttatcgtc cgaaccggtt atacccgctt
    37321 ttgatgcatg tctgaccgcc atttcatgct cgtgtgcagt ttctttgaag aatttgcgca
    37381 ccaccacata aaaaagcgga acaaggaaca cggacaagag cgtgccgatg agcatccccc
    37441 agaatacggt cgtgccgatg gcgcgctggc tggcagaact cgcaccagcg gcaatataca
    37501 ggggaaccac gcccaaaata aaggcaaacg aggtcatgat aatcggacgg aaacgcaggc
    37561 gggctgcctc caaagcggct tcgaccgcgc ttttcccttg cgcttgaagg tctttggcaa
    37621 attcgataat cagaatcgcg tttttcgcac tcaaacccat cacagtaacg aaaccgactt
    37681 ggaagtagat gtcgttggca aacgagggaa cgctgcccaa cagtccttca aacaggttgc
    37741 gcccggttac gcccgcagcc gcaccgatca aacccaacgg aatcacaagg atgaccgcca
    37801 gcggaatcga ccagctttca taaagcgcgg caagtaccaa aaatacggct gcaaccgcca
    37861 aaccgtacaa aatcagggtt tgcgagccgc cttttgcctc ttcgcgcgac tgtccgcccc
    37921 attcgaagct gtaaccgccg cccaattcgt caaccatttt ttgaaccgcc gccatagcct
    37981 gcccggtgga aacgccggtt gcaggcgaag cggacagctt catcgaggga taaccgttga
    38041 agcgtacgct ctgttccgta ccgttttccc aagaaacagt agcaatggtg gaaagcggta
    38101 cggcgacgcc ggatttgttc ggcacggtca ggttcaaaat atcggcaggc tgcatacggg
    38161 catcctcgtc ggcctgcacc atcacgcgtt gcagacggcc ttggttcggg aagtcgctga
    38221 cataagacga actcagcgcg cttgccaatg cggtgcggat gtcggcaaac gaaatgcctt
    38281 gcgccgccgc tgcggcacgg ttgatgtcga ttttcaactg cggcgagtct tccaaaccgc
    38341 cggcacggac ggtgctgggg tcaaacaaac cgctggcacg cattttctga atcagctcgt
    38401 tgcgcttcgc cagcaatgcg gtatggccgg tattgttgcg gtcttgcagg ttgatgctca
    38461 ggcccgaacc gttgcccaac tccagaatcg gaggcgggac gacggcgatg ccgaaaccgt
    38521 ctttaagcgt ccccatcatc atacccgtca gcttgccggc aaccgcaacg gcatcgctgc
    38581 cggaggcggt acgttcgttc caatctttca atatggcaaa acccatcgcc atattctgac
    38641 cgctgcccga aaagctgaag ccggaaacgg taatgatgtt ttcaatttcg ggaatgcttt
    38701 tcgccagttg ggtaacttgt gctagcgtcg catcggtgcg ctctttggtc gcacccgcag
    38761 gcagttgcac gctgaccata acgaagcctt ggtcttcggt cggcaggaac gaagtcggca
    38821 ggcgcataaa caggaacacg cccacaaccg ccaagccgat atagacaacc atcatgcgga
    38881 aagtcttacg cagcactttg gcaacccggc cttcgtagcc gtgcgtccaa ctgtcgaatt
    38941 tcttgttgaa ccagccgaag aaaccttttt tctcttcgtg atgccctttc gggattgtct
    39001 tcaacattgt ggcacacaaa gcaggggtaa gggtcagcgc aaggaaggcg gagaatgcga
    39061 ttgatgacgc catcgtcagg gcaaactgtt tgtaaatatt gccggccgcc ccgctgaaca
    39121 tcgccagagg cacgaacacg gaaatcagaa cggcggtaat accgatgacc gcgcccgaaa
    39181 tctgacccat cgcttttttg gtcgcttctt tgggcggcag gccttcaccc gccataatgc
    39241 gctcgacgtt ttcaaccacc acaatcgcgt catcgaccac gatgccgatg accaatgtca
    39301 tcgcaaacat cgtcaatacg ttaatcgaca tccccatata agagatgaag gcgaaaccgc
    39361 ccaacaggga aatcggtacg acgatggtcg gaatcagcgt ataacggatg ttttgcagga
    39421 agaggtacat tacgacaaac accagcacca tcgcttcgat taaagtgtga atcacttttt
    39481 caatcgaaat ttcaacgaat ttggaagtat cgtaaggggt tttccaactc ataccctgcg
    39541 gaaagtattt ttccagcacc gccaagcgtt cttttaccgc ctttgccgtc gccatcgcat
    39601 tgccgctgtt ggacaacatt accgccatac cggtggtatt taccccgttc aggcgggttg
    39661 aggaagaata gtcttccata cccaatccga ctttcgccac atccttcagg taaatattag
    39721 aaccgtcggt attggcgcgg aggatgacgt tgccgaattc ttctgccgta cccaactgcc
    39781 cttgcgccgt tacggtagcc gtaaccgtct gtccgcgcac agcgggaagc gaaccgatgg
    39841 aacctgcgga aatttggata ttctgcgccg acaacgcgct gccgacatcg gcaaacgaca
    39901 aattgtagtt ttgcagtttc ttaggatcga cccaaatccg catcgcgcgt tgcgcgccga
    39961 acaggcggac ctgccccacg ccttcgatac gctgcaattc gggaacgacg ttgcgctgcg
    40021 cgtagtcgtt catctcttcg gttgactgca catccgacga aagcatcaca atcatcagga
    40081 aattggaacg cgccttggat acggttacgc cgtattgctg gacggttgcc ggcagcgtgc
    40141 tcaatacttc ggaaagcttg ttctgcactt ccacctgcgc cagattctcg tcggtatcgg
    40201 gcgtaaaggt caggctcacg ctgccgctgc cgctcgaatc ggcggaagtg gacatatagt
    40261 ccaaaccttc cacgccgttc atattccgtt cgatcacgga aagtacgctg ccttccatca
    40321 cttgtgcgga cgcacccgga taaatggcgt gcaaagtaat ggtcggggcg gcaacggacg
    40381 gatattgcga aaccggcagg cttttaatgc cgaaaatacc tgccgcaata atgaaaatcg
    40441 aaataaccca cgcgaaaatg gggcggtcga taaagaattt agccatcgat gccttcctta
    40501 tttcgcttca gaagcaggtt tggcttcaga tgccgtctga acgccggctt gaggggcggc
    40561 agcttgattt tcagacggcg cccattcttt aggcgttacc tttttcgccc ccgtcatacc
    40621 ggcgatactg atgccttcca caaccacctt gtccccgtcc ttcagacccg acgtaacaat
    40681 ccaattcgta ccctgctgtt gcgcgaccgt tacctcgcgg ggttccatac cgccctgggc
    40741 attcacaatc atcacggtat ctttcgcacc gcgcgttacc gcctgctgtg gaacgatgaa
    40801 tgcattatcc gccgccacct gatccatcag cacacgcaca tacaggccgg gcatcaggat
    40861 attctgatcg ttcgatacgg cggcgcgcaa ggtaatctga ccggtcgatt cgtcaacggt
    40921 cggatcagca aacagcaggc ggcctttttc aggataaacc gtaccgtcgt caaatttgat
    40981 gccgaccgca atcgcaccat ccgccgccag cagcttgcct tcggctatct gccggcgcag
    41041 tttcatcact tcggatgcag actgggtaac gttcacatac atcggattgg tttggcggat
    41101 ggtggctaaa acagttgtat cgcccgcatt caacagcgta ccttcggaaa ctttggactg
    41161 accgataaag cccgaaatcg gcgcggtaat gcgcgaacgg ttcagattga tgccggcgga
    41221 tttgatcgcc gcctgcgccg ctttaacgcc cgcctcggca gaacgtttcg ccgttaccgc
    41281 agcatcgtac tcttgtttac tgatggcatc ggcggaaacc agcggtttgt aacgcgccaa
    41341 atccgcatcc gctttggcaa gcgttgcctg tgccgttgcc agttgcgcgc gcgcgctttc
    41401 cagacctgct tcataagtgg aactgtcgat ctgatacagc ggctgcccgg cgcggacata
    41461 actgccttct tggaacaggc gtttttggat gatgccgccg acttgggcgc ggacatcggc
    41521 ggtacgcagc gattccaaac gccccggcaa ctcgacggtc aatgcgacgg tttgcggatg
    41581 gacggttacg acgccgacga cgggcgcggg ggcttcccga cccgcaggct gcccgccctg
    41641 cgccgcgtct cctcctttac cgcaagacga cagtgccaat gcaacggcgg cagccaacgc
    41701 ggccgcacgc atcgccttag aagcataaaa agccattatt tatcctatct gtctggtttg
    41761 atgtaaaggg ttttgccaat caacaggcat tcttatttca ggatataaaa accgcctgct
    41821 ttgatacccg aatgttcgaa cgggttgcaa agcaggttat acctgttttc aaagttgaga
    41881 tgcagtctca attttatggg tttcattata catacacgat tgcacggata aaagtctttt
    41941 ttataatccg ccctcgtcaa accgacccga aacgaaaacg ccattatgag aaaaaccaaa
    42001 accgaagcct tgaaaaccaa agaacacctg atgcttgccg ccttggaaac cttttaccgc
    42061 aaagggattg cccgcacctc gctcaacgaa atcgcccaag ccgccggcgt aacgcgcgac
    42121 gcgctctatt ggcatttcaa aaataaggaa gacttgtttg acgcgttgtt ccaacgtatc
    42181 tgcgacgaca tcgaaaactg catcgcgcaa gatgccgcag atgccgaagg aggttcttgg
    42241 acggtattcc gccacacgct gctgcacttt ttcgagcggc tgcaaagcaa cgacatccac
    42301 tacaaattcc acaacatcct gtttttaaag tgcgaacata cggaacaaaa cgccgccgtt
    42361 atcgccattg cccgcaagca tcaggcaatc tggcgcgaga aaattaccgc cgttttgacc
    42421 gaagcggtgg aaaatcagga tttggctgac gatttggaca aggaaacggc ggtcatcttc
    42481 atcaaatcga cgttggacgg gctgatttgg cgttggttct cttccggcga aagtttcgat
    42541 ttgggcaaaa ccgccccgcg catcatcggg ataatgatgg acaacttgga aaaccatccc
    42601 tgcctgcgcc ggaaataatc aagccttggt aacaatgccg tctgaaacga acaaaccctt
    42661 tcaaacggca tcaaaatgac acaaagcatt cttctaaaaa tacatattca ctaaattgca
    42721 tttttaattt cccctatcat cgcatggata ttctcttggt caaaatgtcc gttttcttct
    42781 gaataaactt ctaacaaata atgttcaatg aacgttttat ctgtcatcaa cgatacatct
    42841 ttggcaatgt cttcatacga ctcaaaatca tcttcatgcc atggatcata tttatccatg
    42901 attttttgaa tttcattttt catatcattt accttccaat atttatttac aattaataac
    42961 aataccattc gaatgtaaac tgcatttttc tccggcattc ttgcaaacaa aaaccgaaaa
    43021 tcccgtcatt cccgcgcagg cgggaattcg tttttttgag ttccagtcat tcctgataag
    43081 gctttaacgt caagttttcg gattaccgcc tttatgagaa taacgatgtg ggcattttcc
    43141 gttttaatct attacggtta tatacatata cgattatttt agtttgctta caaaacactt
    43201 catgttaaat tcaaaaattt aatgcactca atatattttt ttaaggagaa gcagatgagt
    43261 caaaccgacg cgcgtcgtag cggacgattt ttacgcacag tcgaatggct gggcaatatg
    43321 ttgccgcacc cggttacgct ttttattatt ttcattgtgt tattgctgat tgcctctgcc
    43381 gtcggtgcgt atttcggact atccgtcccc gatccgcgtc ctgttggggc gaaaggacgt
    43441 gccgatgacg gtttgattca cgttgtcagc ctgctcgatg ccgacggttt gatcaaaatc
    43501 ctgacgcata ccgttaaaaa tttcaccggt ttcgcgccgt tgggaacggt gttggtttct
    43561 ttattgggcg tggggattgc ggaaaaatcg ggcttgattt ccgcattaat gcgcttattg
    43621 cttacaaaat ccccacgcaa actcactact tttatggttg tttttacagg gattttatcc
    43681 aatacggctt ctgaattggg ctatgtcgtc ctaatccctt tgtccgccgt catctttcat
    43741 tcgctcggcc gccatcctct cgccggtctg gctgcggctt tcgccggcgt ttccggcggt
    43801 tattcggcca atctgttctt aggcacaatc gatccgctct tggcaggcat cacccaacag
    43861 gcggcgcaaa tcatccaccc cgactacgtc gtaggccctg aagccaactg gttttttatg
    43921 gcagccagta cgtttgtgat tgctttgatt ggttattttg ttactgaaaa aatcgtcgaa
    43981 ccgcaattgg gcccttatca gtcagatttg tcacaagaag aaaaagacat ccggcattcc
    44041 aatgaaatca cgcctttgga atataaagga ttaatttggg caggcgtggt gtttattgct
    44101 ttatccgccc tattggcttg gagcatcgtc cctgccgacg gtattttgcg tcatcctgaa
    44161 acaggattgg ttgccggttc gccgttttta aaatcgattg ttgtttttat tttcttgttg
    44221 tttgcgctac cgggcattgt ttatggccgg ataacccgaa gtttgcgcgg cgaacgggaa
    44281 gtcgttaatg cgatggccga atcgatgagt actttgggac tttatttggt catcatcttt
    44341 tttgccgcac agtttgtcgc attttttaat tggacgaata ttgggcaata tattgccgtt
    44401 aaaggggcgg tgttcttaaa agaagtcggc ttggccggca gcgtgttgtt tatcggtttt
    44461 attttaattt gtgcttttat caatctgatg ataggctccg cttccgcgca atgggcggta
    44521 accgcaccga ttttcgtccc tatgctgatg ttggccggct acgcgcccga agtcattcaa
    44581 gccgcttacc gcatcggtga ttccgttacc aatattatta cgccgatgat gagttatttc
    44641 gggctgatta tggcgacggt gatgaaatac aaaaaagatg cgggcgtggg tactctgatt
    44701 tctatgatgt tgccgtattc cgccttcttt ttgattgcat ggatcgcctt attctgcatt
    44761 tgggtatttg ttttgggcct gcccgtcggc cccggcgcgc ccactttgta tcccgcacct
    44821 taaacacgat aaacaaaata ccgtctgaaa accttttttc tgttttcaga cggcatttgc
    44881 ctttccatcc cgtcaggctt ctccggtccc ttccctcttt tccgctgcgg caagcgtgtc
    44941 ggcgagcagg cgggcgaggt tttcaaacag gggctgttcg agcgggtttt ggcttgcgtt
    45001 gccgaacacg agggcgactt cggtatagtc tttctgccct ttgctgactt tgctgccgta
    45061 ataggcggtt tgggcttttt tcagggcggc ttcccaatct tgtttttggg caaacgcttc
    45121 ggcggcggca agcgaggttt tggcaaaaaa cggcagcggg cggttttgcc cgatattgaa
    45181 aaacgccatc cattccgaca acagctgcaa tgccctgtct tgcgcgattt ccgccaatac
    45241 ttctgtttct ccggattgga cgataaaggt ttgccgcgtt tcggcttcag acggcataac
    45301 ggcgcaaaat atcaggtgtt ccaacaaaaa agccacacgt tgcggcgcat tgggtttgcc
    45361 gtaggcgtaa aacacctgac cgcagcggta cagattgccc aagctgcctt tcaggatttg
    45421 cccgtccgac ggtatggcgt atgggaacgg aggaagtttt gggctgttta aaaccgacgt
    45481 gtcgatttgt ttggcggcgg tttggaagga ctgctgccaa agttttccca attctccgga
    45541 aggcagcagg ctttccgccg cgatacgggc ggcggtttgg gaaaaatctt gtccttcgcg
    45601 gcgcgccccg atataggttt cggcgatttg ctccgcgtgt tgcggctcga agggttcggc
    45661 aggctcccag gcttcgccga tatggggttc gctccacgca agctgctgca gccatacttt
    45721 gacggggttg cgccagaagc ggataaattc gtcctgtccg atttcggcaa caggttcggc
    45781 gttttctacg ggttggtcga aaaagggttg cggcggttcg ggcgtttgtc cgagcgcggc
    45841 ggcgtagtcg gtacgcgtgc cgaatatgcc gtctgaacgt ccgccttctt ggaaatatcg
    45901 gcgcgagaag gcttgcagcg gatgctgttc tatccaattt tgtgcaagtt ggcggctgtt
    45961 tgtgccggtc atggcggcaa ctgtgtcgat gagttcgccc aacagggaag acggggcaag
    46021 ctcttcgtct ttgcggatgt cgcgcccgat gtaggacaaa tacaggattt cgcgcgcgct
    46081 gatgagggct tcgaggaaca ggtagcggtc gtcgtcgcgg cgggcgcggt cgcctttggc
    46141 gggatgtttg gcaaccaggt cgaatacggc ggctttggta ttgcggggaa aatctccgtc
    46201 gttcaaaccc aacaggcaga tgactttgaa cggcaggctc cgcatcggca ccatactgca
    46261 aaaggtgatg ccgccgcgta aaaagcctgc ctcgctttcg ctgtcgagga agcggcggat
    46321 atggcggatg acggtttgtt gcggcaactg tccgaaaaac ccagccaaag tgctctcttc
    46381 ctgccatttg acccattcgt tttcgaggtt ttggactgcc ttttggtcat cgggcccggc
    46441 ttgaaacaat gtttcaagca aatcccggca acgcgccacc cattcgccga ccgtcgcagg
    46501 ttgccgccat atccgggcaa tatccgacag ggtttcgagg aaggcggcaa aacgtccgaa
    46561 catggcggtt tgattcacgt cggcatacca cgcgctgaca tcctgccaca tcgggttgcc
    46621 gccttcgggc agcatccagc ccaatatcat gcgttctacc gcctgcttcc aggtaaacag
    46681 ctgatccgtg ccgccgcgca tttctccgtc caaaccccag cggacgttca aatcggcaac
    46741 catgtcgtgc aaaagcggta aatcgtcccc ggtcagtccg aaacggcgca acacgggcgc
    46801 ggtttccaaa agcgcgagca ctttgccgac ttcaaaacgg ctttccagca agtcggacac
    46861 gcactccaac gcataaaaaa acggttgccg gcggctaatt ttcacgtccg aaacggaata
    46921 cggcaatgcc tgcgcgccgg gctgcgcctg tccgaacacg gcttcgataa aaggcgtata
    46981 gggttcgatg ttcggggtta atacggcgat gtcgtgcggc tgccaatcgg gatgttcatg
    47041 cagaattttc aacagtttgt ctttgagtat ctgcaattcg cgcaaagggc tgtgtgcgga
    47101 gacgatgcgt atcgagccgt cgcccgtgtt gacgcttccc accgtttcag acggcatttt
    47161 caggttttga atatcggttt gcagggcgtg caaaagcgta tcgcgcccgc cttcttcaaa
    47221 taccggcgtt tcttcttcta tttccatttc gttcaaaaag tcgaaaaagt cgcgcccctg
    47281 cttgcccaat gaggcaagca gcggatgccc tgcctgcgtc agatcgggat cgccgccgcc
    47341 tttgaggatt tgcgccgctt cgatgacgtt gccccaatgc atcccgctcg gattgagtgc
    47401 gaacacgaac acgtcgcaat gttcggacag cttgtgcaaa agctgcaaat acatcggcgc
    47461 catcgtggaa atgccgaaca cgaaataacg ctcgggcagc ttatccttgt ccaacgcggc
    47521 aagcagtttt tcccacaacg cgacacggtg cggcgcgctc tgcctgccgt cgtcgaggta
    47581 acgccacagt ttggactgcc agatttcgtc gtcgcccaaa ccgagtatcc tgccctgctg
    47641 ccaagcgtct atccactgag gacggtacac gaggtattgg tcgaatatgt ctgcaagctg
    47701 tcccgcaagc tggtaatccg ccgattctcc gctgcccaga tagtcttgca gcacattcct
    47761 cacatcttca aattccgctc cgttttgaaa ttctgcgctg cggaacaaat ccagcagccg
    47821 ccagcgcatg acttcgggtg caaacgggct gagttccgga ataccgggaa tcagtttttt
    47881 catcaacttc cacgtcaggc cggcgggcag gctgaacgcc aaattcgccg ccacgcccaa
    47941 atcgcgggcg aggcaggtgt tgaggtagcg gcgcatcccc tgactctgca caacaatctg
    48001 ttcgggctgc aaggcacatt tcagcggttt gactttttga atgcgggcaa acagtgcagc
    48061 gagcgattcg aggcggttgg attggtacag gtaaaacatg gcttgctttt tcaaaatgac
    48121 agtgatgcgg aattatatag cttctcgggc aagccgcccg caaacaaaat gccgtctgaa
    48181 cgcttcagac ggcatcgggt catttaaacc atttcctcaa cacaggaatc cgcgacagca
    48241 gcaaggtatc caacagccaa atcacggcaa tggcaagcag cgcggtcggg aaaagcagtg
    48301 caatcaacag cagcggcaat gccatcgccc accagacggg cagtttgatt ttttgcgccg
    48361 gaggaacaat gcccgccacg ccggacggac ggcgtttcca ccacatcacg cagccgctga
    48421 tgccgataaa aatcacggca aggcagaaca cgacgttcgc caacacgctc caccagccca
    48481 aagtccccat atgcagcgca atgcttgccg ccataaattt gccgaacggg ttgtaatcgt
    48541 caaaacggat gtcggcaaga atcttgccgc tgtactggtc gatatgtacc gtgcggtcgg
    48601 caaacgggct gatcatgtca taactcatag aatcctgcga caaagtccat accccgtcct
    48661 cgcctttggg caaattcaac tgataacgcc ctttgaaacc gatttcccgc gcgaaacggt
    48721 cgacggtttc caatgtcatc ggctcgctgg ggttaatgcc gttttcaccc acagtcgtcc
    48781 ctgagacagg cataggcgta agctccaaaa tccacggcac ttccttaacc ttgccgtcat
    48841 tcaatacctc gccgtgggtc ggcacgactg aaacggggtt cggttcgaca ccccatttgc
    48901 cggccgggaa ctgactccaa gcctgcacga acttgccgcc ccaaatacct gcccaagcaa
    48961 tacccgacag gcagaacaac agtaaaatca acgacaccca agttccaaac gcgccgtgca
    49021 gattccgcca ccaagaacgc gccctgcctt ttggcggcag cagcatcgct ttaatgccgc
    49081 gctgtttcgc ccaccaaagg tacaagccgc tgacaaccat aataatggtc agtgaagctg
    49141 ccgtttccaa aagataatcg cctgccgcac cgagcatcat atcgccgtgg atttcatcca
    49201 tagtgtgata ccaaccctga ttgcgcggca tggtgttgac cacttttgcc gtataaggat
    49261 cgaccgcaac catggttgcc ttaccctcat tgttgacacg gaacacagca accatatcat
    49321 cggcacgcgg tgcaatatat tggacgacgg acgaagtctc cggattaatg gcaccgcgtg
    49381 ccgcttctgc ctgaacagag aggggttgca ccactgcctg agggaaaacg tgaatacgct
    49441 ctccctcttt accggtaata ttggcaaaca acagcatacc caaacctgta acggcaagca
    49501 gggtcaagaa gggcataacc aataaaccgg cataaaaatg ccacctccaa acgctcagat
    49561 aatgccggtt attctgctga ccggcttcag ttttgatttg tgtatccatt aatcgtcctt
    49621 ttgaaattaa ggctatcgtg atgatgcgcg attataaaca ataaagacta attctttatg
    49681 actaaaatca atttttttgc aacatatgaa aaaagattca attttgaaga aacaaaccta
    49741 tatgccgtct gaaagcatca aacaatgctg tccgacaccc aactgcacca ttctttaaac
    49801 catacaaaaa tatcagtcgg tctgactgct taagcatcaa aatccccact ttcagacggc
    49861 ataaatcaaa aacaggattt ccgttcaaaa taaaaaatcc ggattcgata tgaatccgga
    49921 tttctgcatc cgacaaagtc ggaaaacaac ggttagaact taccgcctga ctggttgacc
    49981 atatagtcaa ccgcagcttt gacctcatca tcgctcaaat cgccgcgacc gccttttgcg
    50041 ggcatcgtat tgaaaccttc gatcgcgtgt ttgtgcaacg tgtccttacc ttttttgatg
    50101 cggtcggccc agtcggcttt ggtgcctaca tggggaatac ctggaattgc attgccatgg
    50161 caggcggcac aaacggtttc ataaacctgt ttgccatccg ctttggcggc aggtgcggct
    50221 ttttcctcgg ctttaggttc tgctgcggca ggtttggctt cagaagcggc ttgtgctgct
    50281 cctgcaggag cagatggtgc gggttctgcc gccggtgcag gctcggcttt tttcaccgga
    50341 gctttaccgt ctttatcgga aagaccccat acataagcag tcatgatatg cagtttgtct
    50401 ttatccaaga aatgtcccca agcgggcatt tggctgctgc gaccgttggt aatggtttcg
    50461 ataatggatt tttgcgtacc gccccacaac cacacgtcat cagtcaggtt cggacccaaa
    50521 ccttggatac cttgtccctt atcgccgtgg caagtgaaac agttggcagg cggaccgctg
    50581 aacaaggctt gtccgcgcgc ggcacgttcc tcatcatact gacctttggg ttttgaaagg
    50641 gacatcacat aatgggcaac gtctttcacg ccttcttcgc ccaaagcagg accccaggca
    50701 ggcatagtcg caacacggcc tttttcgatg gtctcgtgga ttttatcggg atcgccgccc
    50761 cacaaccaat cgctatcggt cagattcgga aaacctttag aacctttggc atcagagccg
    50821 tggcactgga tacaataagt gttaaacagg ttttgggcga tttgcttggc ctgtgggtct
    50881 tttgccactt tttcaatcgg catatccgca aacttggcat acagtttgcc gtattgctca
    50941 tcggcttttt tgacctcttt ttcatattgg ttatggctgg tccatttcag cagacctttg
    51001 tagtcgccga cacccggata cataaccaga taaccgatac cgaacagcca cgtcaaaaca
    51061 tacagccaaa accaccagcg gggcagcgga ttgtcgtatt cggcaatgcc gtcccactcg
    51121 tgacccgtag tttgtacttc ttcgcccttc ttcggacgtt tgacaacatt ttgagacagc
    51181 agcagccaag ccaaagcgat aaagctcagt aagacaataa ctgcaatata tatattccag
    51241 aaattactgg taaattggga tgttgcgttc attgttttgc tccgttatca caatattaac
    51301 ggttttcgct tttcttatct tgcgcatctt ggttttcagc aaaaatgctg tttgcggcat
    51361 catcgtagtt tttcttattc cgcctgttga agacgatata gagcactaac aggaaacaga
    51421 taaagatcca taccgtgaaa agagcgcgaa taccgttaat atccatgatg ttaccttacg
    51481 tttttcaaag ccagacccaa tccttgcaga taggcgacta cagcatccag ctcggatttg
    51541 tttgccaaag cctcaggcgc tttcgcaatt tcctcatcac tgtaaggagt acctacttta
    51601 cgcaaagcct tcatgttggc aacggttgca tcgacatcga ctttattgcg tgcaagccac
    51661 gggaatgccg gcatattgga ctcaggcacg acatcacggg gattcagcag gtggatgcgg
    51721 tgccattcgt cggaatagcg gccgcccaca cgtgccaaat caggaccggt acgtttggaa
    51781 ccccattgga acggatggtc gtaaaccgac tctccggcaa cagagtaatg accgtaacgc
    51841 tcagtttccg cacggaacgg acgaatcatt tgagagtggc agttgtaaca gccctcacgg
    51901 atgtaaatat cgcgtccggc aacctgcagg gcattgtaag gcttcacgcc cggcgccggc
    51961 tgtgttgccg ccttggtaaa ggccaagggc acaacttcaa tcaacagacc gacactgact
    52021 acaagcagcg tgaacacaat cagaacgccg attttttctt cagccaattg ttgtaatttc
    52081 attttggtag cctatctttc ttagtatttt agtggtgctg tgtttgggaa accgcaggga
    52141 tttcggcatc gactgcttta ccaccgatgg ctgtgcggta cacgttgtac gccataatgc
    52201 acatgccact cagatacaat aaaccacctg caaaacggat catgtagtaa ggcatggtgc
    52261 gttttacgga ttcgacaaac gagtaggtca gcgtaccgtc atcgttcaga gaactccaca
    52321 tcaaaccctg catcacacct gcaatccaca tcgcggcgat atacagaacc acaccgatag
    52381 tcgcaatcca gaaatgcgcc tctaccagct tggtgctgtg catctgctct ttgccgaaca
    52441 gacggggaat catgtaatag acggaaccga tggttacaaa gcctacccag cccaacgcac
    52501 ccgcatgaac gtgcgcgacg gtccagtccg tatagtggct caatgcattg accgttttaa
    52561 tcgacatcat cgggccttca aaggtagaca taccgtagaa ggacaaggat acaatcagga
    52621 atttaagaat cgggtctgta cgcagtttgt cccacgcgcc ggacaaggtc ataatgccgt
    52681 taatcatacc gccccaagag ggtgcgaaca aaatcaagga cagaaccata cccaaagatt
    52741 gcgtccagtc aggcagcgca gtgtagtgaa gatggtgcgg acccgcccac atataggtga
    52801 aaatcaacgc ccagaagtga acgacggaca ggcggtagga gtaaacgggg cgggctgctt
    52861 gtttgggtac gaaatagtac atcataccca aaaagccggc agtcaggaag aagcccacgg
    52921 cgttatgccc gtaccaccat tgaaccatag catcaatcgc accggaatag acggggtatg
    52981 acttcatcaa accggccggg atgctgatat tgttgacgat gtgcaaaagt gcgaccgcca
    53041 aaataaagcc gccgtagaac cagttggcaa cgtaaatgtg ttttaccttg cgtttggcaa
    53101 tcgtaccgaa gaatacgatg gcgtaagcca cccaaaccaa agtaatcaga atgtcaatcg
    53161 gccattccag ttcggcatat tccttacctt gggtccaacc catagggaag ctgacgacgg
    53221 cggcaacgat taccgcctgc caaccccaaa aggtaaatgc cggcagccaa ccgccgaaaa
    53281 gacgggtatt acaagtacgt tggacaacgt agtatgatgt gccgatcaga ccgcaaccgc
    53341 caaatgcgaa aataaccgca ttggtgtgca gcggacgcag gcggccgaag tggaaccaag
    53401 gtccgatatt agacaagtcg agggcaggag caaaaagctg ggcggcgacg ataacgccga
    53461 ccaacatgcc cacaatcccc caaactacag tcatgatggc gaactggcgc accactttgt
    53521 agttgtaagt ttgtgtgtcc atgagagtct ccatgaatta tgggaataaa gatttttatc
    53581 ctgccgcttc cgcagcctgt ttaaggtgca atccgggcaa gcataatttt ttctaaattt
    53641 aacatatctg ccttattacg ccaagcggaa ttacattcgc gccgccggcg aaccctttgc
    53701 ttaatctgtt ttttattaca tataaatcat attgttataa taaattacaa cccggccgcc
    53761 attgcttttg tttccaattt tccctttttg tggcacttta ttgatgtagg ttaagctgca
    53821 ttttaaaggc atttaatcca tcccgtttaa cgatatattt gatagttatg attcattata
    53881 aaatagcccc gtcccctcta gaccatgaat ggcacatcct gctgaaattc acacaagata
    53941 atgattttcc tatagaaata agccttccga attgggtgcc gggcagctat ctgattcggg
    54001 atttttcccg ccacatcact tctatccatg catcctgcaa cggcacgtcc atgccgctcg
    54061 aacaaattgc aaaaaaccgc tggcacactg ccgccgtacg cggcgagtgg caaatccgct
    54121 acaccgtata tgcattcgat ttgtcggttc gaggttcttt cctgacgaca gaacgcggtt
    54181 tttttgacgg atcgtgcctg tttttgaaaa tcgaaggaac ggaaacgctg ccgcaccgct
    54241 tggaattgac aggcattcca cccgaatggc gtattgccac aacgctgccg gaaacaggga
    54301 ggcttgtctt tcagacggca tcttatgacg aattgattga ccgacctgtc gagatgggct
    54361 tgattgaatt tttagatttt gaggcggcag gcattccgca cacgattgcc ttaagcggca
    54421 tatatcccga tttcgaccgc gacaggctgg tttcggacat caaaaaaatc tgcgaaacag
    54481 aactggcgat gttctcctcc cctgccccat ttgagaaata tttgttcctg ctccacgtcg
    54541 gcgaccatat ttacggcggt ttggaacaca ccgacagcac cgcactgctc gccgatcgcc
    54601 acagccttcc gccgtacggt atgaccgatg ccgacgatgc ctacaccaca ttgctcggac
    54661 ttttctccca cgaatatttt cacgcgtgga acgtcaaatc catcaaacct gccgcattcg
    54721 ccccttatga cctcgacaaa gaaaactata ccgaacaact atgggcattc gaaggcatta
    54781 catcctatta cgacgatttg tttttggcac gcagccgcac catctcgccc gaattttatt
    54841 taaacctgct agcacaaggc attacgcgcg tacaacaaac ccgcggccgt ttgaggcaga
    54901 ccttggcgga atcgagtttt accgcgtgga acaaatttta caaaccggat gaaaacagcc
    54961 ccaacgccat cgtcagctac taccaaaaag gcgcacttgc cgcattgtgc cttgatctga
    55021 taatacgcaa ccgaagcaac ggcagacatt cccttgatac ggtaatggac aaactctatc
    55081 gggagtggag ggacacacac tcgggtattc cggaaaaaca ctggcaaatc cgttgtcagg
    55141 aaattaccgg cttggatttg acagattttt ttcagacggc attgtacagc accgaagatt
    55201 tgccgcttgc cgaatgcctg gcaaccacag gcgtgaaact gaccttcctg ccgcttcccc
    55261 gacaacacgg cggcggatat gcggaacaca ttttccccat cccgccgacg ggcgatttcg
    55321 gcgcacgctt caaacaaaac gccgaccata tcgtcctgac ccatgtcttc aacggcggaa
    55381 gtgcggaatc tgcggcactg tgcccgcaag acaaaatcat tgctttagac ggttatgcct
    55441 gcaccgactt tgccgcacaa tgggcccgat accacgtcag ggcaaaaatc aatatccact
    55501 tcttccgtgc aggcatattg cgtcaaaccg tcttgacggt tcaggcaacg gcggcggata
    55561 ctgccatcct acacatcaca gatcgcaacc tgttggaaaa ctggttgttc ggttaaactt
    55621 tcagacggca ttgcacacaa aatgccgtct gaacaccaac cgcaaagcaa aggaaacaaa
    55681 atggcgattc tcaaacttga cgaacatctc tacatctccc cgcaactgac caaagccgat
    55741 gcggaacaaa tcgcgcaact tggcatcaaa accgtcatct gcaaccgccc cgaccgcgaa
    55801 gaagaatcgc aacccgactt cgcccaaatc aaacagtggc tggaacaagc aggcgttacc
    55861 ggattccatc accaacccgt taccgcacgc gacatccaaa aacacgatgt cgaaaccttc
    55921 cgccaactca tcggacaagc cgaatatccc gtccttgcct attgccggac cggtacgcgc
    55981 tgctccctcc tgtggggctt ccgccgggcg gcagaaggta tgccggttga cgaaatcatc
    56041 cgccgcgccc aagcggcagg tgtgaatctg gaaaacttca gagagcggct ggacaacgcc
    56101 cgcgtctgat tacaagccga aacgtttaaa ccacaccttc aagcggcatt ccaccgcaac
    56161 ttgaaaaaga ggacggcaaa ccttactgcc gtcctctgtc cttctccgtt tttacagcgg
    56221 gtatagcgga ttaacaaaaa ccagtacggc gttgcctcgc cttaactcaa agagaacgat
    56281 tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatttgtact gtctacggct
    56341 tcgttgcctt gtcctgattt ttgttaatcc actataaaaa ttagaaatgc acattttcat
    56401 tattctcgcg caggcaggac tccagactta cccatttcag taatgtttga aaataaaaga
    56461 aaaatcagat gtttgtattc ccgcctgcgc agaaatggag acggtgctct gtcgtctcat
    56521 ttttgtttta atcaactata tataactgat taaacataag aaatgccgtc tgaaagactt
    56581 tcaggcggca ttcgttcaag cgtcgaactt tattgcgcct tggtttcggt tacaaaaccg
    56641 attttggtaa ttcctgcctg acgggcggct tctaaagctt tgtttacata atcgtattcc
    56701 accgccttgt ctgccgcaat cgccacaatc acgttttcat tctgctcctt ggcggctttc
    56761 agacggcttt ccacttcccc gatttccact ttgcttgcag aatccccgcc gacataatag
    56821 ccgccgttcg catcaatcgt caggcgcagg gggtctttag gctgtttgtc ctgcttgttt
    56881 gtctgctcgg acgcggtcgg cagttccaaa gggatggaat gcgtcagcac cggcatagta
    56941 atcataaaca caatcagcaa caccagcatc acgtccacca acggcgtaac gttgatgtcg
    57001 gacatcggag aatcgtcgct ggaattcatc gaaccaaatg ccataatcag ctatcctttt
    57061 gattaagcag gcggacgtgc aaatcgtgcg ccatcgcatc caaatcctgg gtcagtattt
    57121 ttgtgccgcg attgaggaag ttgtatgcca acaccgccgg aatcgccacg aacaaacccg
    57181 ccgccgtcgc caccagtgcc tcgccaatcg ggccggcaac cgccgcaata ctcatctgcc
    57241 cgctttgccc gatattgatc agggcgtggt aaatccccca aaccgtgccg aacaacccga
    57301 taaacggcgc ggtcgcgccg atggaggcaa gcgcggtcat cccgtaatca aaccggcgca
    57361 taatctgcgc catactgttg cggatttgaa tgaccaaata ctcgttcaac ggcaaagcct
    57421 gcgccagttc ggacgcttcg tttcggcggt agttgcggta agactgcaat gcctcttgcg
    57481 ccagtttgga caaaggcgca tcgacggcgc gcactttttc gaccgcgtcg ttcagcgaca
    57541 aagtatcgcg catatgccgt ttgacggcgg cattcccttt gcgcgcccga tacagcttga
    57601 tgcagcgcaa gacaaccaga caccacgtta cggtactcat caacagcatc aacacaaaca
    57661 caccgatcag gacgggatcg cccgattcaa acactaattt caaattcata atgattccaa
    57721 cactgaaaaa accaatcaaa catccaagct gccgcaaact gccgcgacaa ccgcctaatt
    57781 caattcaaac ttgacgggga ctttaaactc cgtccaggca ttggcttgaa aatgcccgtt
    57841 ttgcgccgct ttgcgtgccg cattgtccaa acgggaaaaa ccgctgcttt tcaccacttt
    57901 gacggactcg acatgaccgc ccggagaaac caaaacgctc aaaacaacca taccctgctc
    57961 gtcattttcc atagaaagtg cgggataggc cgggcgcggg atgctgccgt tggcgcgtaa
    58021 aggattgcct ttgctgctgc cgacaccggt tccgccgccg ctgccgccgg ctccttcccc
    58081 gtgttcgcct ttggcaccgc cgcttccttt accgctgcct tctccgcgcc ccgttccgtc
    58141 gcctttggta ccggttccct taccttcccc attaccctgc tcgctgccgg ctttggcaga
    58201 agcattaccg gaatgttcgg caggtttttc agacggcttc tctgccggtt tttccgccgg
    58261 tttcggggca ggcttcgctt ccggttcggg ttcgggctta ggtttttctt cgggtttcgg
    58321 cttttcctta ggttgctgaa tatccgcatc cgcctttttt gtaaccgccg gcttcaaaac
    58381 cggcttgggc ggttcgacag gtttgggcgg atcgggcgcg ggttgcggtt cgggcgcggc
    58441 aggcgcgcct gcgccttcgg gtgcgccgcc ccctccgcca aaatcgccca aatcgacaaa
    58501 ttcgataaca tttcctgact ctatcacggg cagcttgtgc gcctgccaga gcaatgccac
    58561 tattgccaaa tgcagcagtg cgacggaaaa cacgactgcg ggggttaaaa ttcgttcttt
    58621 atccataatt cgggcataat aatagcaaca attcctattt gcaacctatt ttttataatt
    58681 tgttgtcatg tgaacgcctg ctccattcat aggcaaaccg tgtttaaacg cggtattaca
    58741 gcaaatcatc agataacggg ccggcagaaa aaatgattcc gtctgatttc ttattccaat
    58801 aaaatcaggt tagatgatat attgccgctt ctgtctgtca gccgtttcgg gctgcacacc
    58861 acatctgttc aaaggaaaac catgtttcaa aattttgact tgggcgtgtt tctgcttgct
    58921 gtcctgcccg tgctgctcgc cattaccgtc agggaggtgg cgcgcggcta tacggcgcgc
    58981 tactggggag acaacactgc cgaacaatac ggcaggctga cactgaaccc cctgccccat
    59041 atcgatttgg tcggcacaat catcgtaccg ctgcttactt tgatgttcac gcccttcctg
    59101 ttcggctggg cgcgtccgat tcctatcgat tcgcgcaact tccgcaaccc gcgccttgca
    59161 tggcgttgca ttgccgcgtc cggcccgctg tcgaatctgg cgatggctgt tctgtggggc
    59221 gtggttttgg tgctaactcc gtatgccggc ggggcgtatc agatgccgct ggctcaaatg
    59281 gcaaactacg gtattctgat caatgcgatt ttgttcgcgc tcaacatcat ccccatcctg
    59341 ccttgggacg gcggcatttt catcgacacc ttcctgccgg cgaaatattc gcaagcgttc
    59401 cgcaaaatcg aaccttatgg gacgtggatt atcctgctgc ttatgctgac cggggttttg
    59461 ggtgcgttta ttgcaccgat tgtgcggctg gtgattgcgt ttgtgcagat gttcgtctga
    59521 ctggctctca gacggcataa acgttccaaa aaacgcggaa ggacatattg ccctgccgct
    59581 tttttttggt tttacaatgc cctgtcgaaa taaagcggca cgcctgattt ttcactcata
    59641 ccgattaaat aatccatcgt cttccgaggg aacgcgatgc cgcgcaccac ggtcagattc
    59701 ctcaaaacgg ggaaaaccaa aatatcctcc ataccgattc caccgttgat gccgtctgaa
    59761 gtaccgtcca tcaaattctc caactcttgc aaatctgcgt ttatccgttc aaggtattgg
    59821 gcggttttat tcaaattggc ggaaaagctg ccgatgcttt tctctttttt gtctgtgaaa
    59881 tatttcaccg cttccggcgt tacaaattcg ggcagcccga ttttgatcaa gcgtggctga
    59941 accaatttgt tgttgtatcc gcctaccttg tccagccacg cctgtatctc ggggcggact
    60001 tcgtctttca gacggccttc tcggtcgaaa tggcacacaa tgtccaaact ctcgcccata
    60061 aacgaaccgt cttctttttg caggacgggc acttgtttcg caccgatcat accgatcggc
    60121 gttgcctcgt cgtcgtttgc cagcacgact tcttcaacgt cagcaccaaa cagcccggca
    60181 gccatccgcg cacgcacgca aaacgggcaa tggtcgtaaa tatacagttt catcaaaata
    60241 ttcctcgtca acctgtcggt accggctacc ttaacacccg gcgccgcccg aaacaagttt
    60301 atcttcccgc ctatgcaccg taaataaata agctgttaca atgaactcgt ttttatcgga
    60361 acggaagatc ccatcatgac cgccatcagc ccgattcaag acacgcaaag cgcgaccctg
    60421 caagaattgc gcgaatggtt cgacagctac tgcgccgctc tgccggacaa cgataaaaac
    60481 ctcatcggta ccgcatggtc gctggcgcag gaacattatc ctgccgatgc cgccacgccg
    60541 tatggcgagc cgctgcccga ccacttcctc ggcgcggcgc aaatggtcga cgaactcgac
    60601 ctgctgcccg atgccgtcgc cgccaccctg cttgccgaca tcggacgcta cgtccccgat
    60661 tggaacctat tggtttccga gcgctgcaac agcaccgtcg ccgagctggt caaaggtgtg
    60721 gacgaagtgc agaaacttac ccacttcgcc cgggtggaca gcctcgccac gccggaagaa
    60781 cgcgcacagc aagcggaaac catgcggaaa atgctgctgg cgatggttac cgacatccgc
    60841 gtcgtattaa tcaaactggc gatgcgtacg cgcaccctgc aatttttaag caacgccccc
    60901 gacagccctg aaaaacgcgc cgtcgccaaa gaaaccctcg acatcttcgc cccgctcgcc
    60961 aaccgcttgg gcgtgtggca gctcaaatgg cagctcgaag atttgggctt ccgccatcaa
    61021 gaacccgaaa aataccgcga aatcgccctg cttttggacg aaaaacgcac cgaacgcctc
    61081 gaatacatcg aaaacttcct cgatatcctg cgtacggaac tcaaaaaata caatatccac
    61141 tttgaagtcg ccggccgtcc gaaacacatc tactccattt acaaaaaaat ggtgaagaaa
    61201 aaactcagct tcgacggcct gttcgacatc cgcgccgtgc ggattctggt cgataccgtc
    61261 cccgagtgtt acaccacgct gggcatcgtc cacagcctct ggcagcccat tcccggcgag
    61321 ttcgacgact acatcgccaa ccccaaaggc aacggttata aaagtttgca caccgtcatc
    61381 gtcggcccgg aagacaaagg tgtggaagtg caaatccgca ccttcgatat gcaccaattc
    61441 aacgaattcg gtgtcgccgc ccactggcgt tacaaagaag gcggcaaagg cgattccgcc
    61501 tacgaacaaa aaatcgcctg gttgcgccaa ctcttggact ggcgcgaaaa tatggcggaa
    61561 agcggcaagg aagacctcgc cgccgccttc aaaaccgagc ttttcaacga cacgatttat
    61621 gttttgaccc cgcacggcaa agtcctctct ctgccaacgg gcgcaacccc catcgacttc
    61681 gcctacgccc tgcacagcag catcggcgac cgctgccggg gcgcgaaagt cgaagggcag
    61741 attgtgccgc tgtccacccc gctcgaaaac ggacagcgcg tcgaaatcat taccgccaaa
    61801 gaagggcatc cttccgtcaa ctggctttac gaaggctggg tcaaatccgg caaggccatc
    61861 ggcaaaatcc gcgcctacat ccgccagcaa aacgccgaca ccgtgcgcga agaaggccgt
    61921 gtccaactcg acaagcagct tgccaaactc acgcccaaac ccaacctgca agagcttgcc
    61981 gaaaatctcg gctacaaaaa gccagaagac ctctacaccg ccgtcggaca aggcgaaatt
    62041 tccaaccgcg ccatccaaaa agcctgcggc acgctgaacg aaccgccgcc cgtgcccgtc
    62101 agcgcaacca ccatcgtcaa acagtccaaa atcaaaaaag gtggcaaaac cggcgtgctc
    62161 atcgacggcg aagacggctt gatgaccacg cttgccaaat gctgcaaacc cgcgccgccc
    62221 gacgatattg ccggcttcgt tacccgcgag cgcggcattt ccgtccaccg caaaacctgc
    62281 ccctctttcc gacaccttgc cgaacacgcg cccgaaaaag tactggacgc aagttgggcg
    62341 gcgttgcagg aagggcaagt gttcgccgtc gatatcgaaa tccgcgccca agaccgctcc
    62401 gggcttttgc gcgacgtatc cgacgcgctc gcccgccaca aactcaacgt taccgccgtg
    62461 caaacccagt cccgcgactt ggaagccagc atgaggttca cgctcgaagt caaacaagtc
    62521 aacgacctcc cgcgcgtcct cgccggcctc ggcgatgtca aaggcgtatt gagcgttacc
    62581 cggctttaaa tacaaaaatg ccgtctgaaa gccggataac gcttcagacg gcattttgat
    62641 tgccggggtt tgctattttt tgttgtaata atcaaatcgc acgttgacta tgtctatctc
    62701 ggtaaaaata taacggagca ttactctgaa cctttcataa cgttcatgaa ttcccacact
    62761 atcaggcaac caaggggaag ctttaatttc aaaaagctcc caatttggaa ccattaaaaa
    62821 atcaataatg ataccgattc caatcacaat atcccttggt atatccatcg gataaggata
    62881 ttttctccta acctcgatta aagcattctc caatttccaa tattcttcat catcccacac
    62941 cccgtcatca taccatttgc caataaatga attttcgtca tactcttcaa aacaagggat
    63001 gtttcttcta aaatccttga actcgcacat aataattaat ctccaatacg atttaggttt
    63061 ttatcaaatg taccgtttct tgtttctttt ctgttcagat ttttgggtga agatgcttct
    63121 ttccaagccc ctccattatg tgaatctaca tcgcgtgata tataactctt tcctttttta
    63181 aaaatagcag catcatttct cgttctttct tttatttttc tatatcccaa tttctttgct
    63241 gctgcatatg cttctgaatc attcccatat acgggggtag atggtgtttt tcttggcgga
    63301 cacgcattat gaacccaaac cccttccgtt tccgcctgat tacccttgac gaagtaggta
    63361 tgccagtcgg caacggtcag attgtaggct ttgagcggtg ttggtttgac aatggtgttg
    63421 c
//
LOCUS       DAXFWM010000004        51666 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000004 DAXFWM010000000
VERSION     DAXFWM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 51666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51666
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.004"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            140..421
                     /locus_tag="ACFI8B_000212"
     CDS             140..421
                     /locus_tag="ACFI8B_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833527.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            418..1461
                     /gene="argC"
                     /locus_tag="ACFI8B_000213"
     CDS             418..1461
                     /gene="argC"
                     /locus_tag="ACFI8B_000213"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC3833528.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
     gene            complement(2055..4097)
                     /gene="recG"
                     /locus_tag="ACFI8B_000214"
     CDS             complement(2055..4097)
                     /gene="recG"
                     /locus_tag="ACFI8B_000214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC3833529.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(4183..4626)
                     /gene="secB"
                     /locus_tag="ACFI8B_000215"
     CDS             complement(4183..4626)
                     /gene="secB"
                     /locus_tag="ACFI8B_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC3833530.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(4647..4904)
                     /gene="grxC"
                     /locus_tag="ACFI8B_000216"
     CDS             complement(4647..4904)
                     /gene="grxC"
                     /locus_tag="ACFI8B_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC3833531.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5123..6616
                     /gene="rng"
                     /locus_tag="ACFI8B_000217"
     CDS             5123..6616
                     /gene="rng"
                     /locus_tag="ACFI8B_000217"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC3833532.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(6651..7902)
                     /locus_tag="ACFI8B_000218"
                     /pseudo
     CDS             complement(6651..7902)
                     /locus_tag="ACFI8B_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(7984..8613)
                     /locus_tag="ACFI8B_000219"
     CDS             complement(7984..8613)
                     /locus_tag="ACFI8B_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC3833533.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(8881..10194)
                     /locus_tag="ACFI8B_000220"
     CDS             complement(8881..10194)
                     /locus_tag="ACFI8B_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC3833534.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILVSSIAAGIVPLP"
     gene            complement(10563..11135)
                     /locus_tag="ACFI8B_000221"
     CDS             complement(10563..11135)
                     /locus_tag="ACFI8B_000221"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC3833535.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            11294..12703
                     /gene="dacB"
                     /locus_tag="ACFI8B_000222"
     CDS             11294..12703
                     /gene="dacB"
                     /locus_tag="ACFI8B_000222"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC3833536.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(12768..13937)
                     /gene="metK"
                     /locus_tag="ACFI8B_000223"
     CDS             complement(12768..13937)
                     /gene="metK"
                     /locus_tag="ACFI8B_000223"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC3833537.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(14120..14230)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            14283..15176
                     /locus_tag="ACFI8B_000224"
     CDS             14283..15176
                     /locus_tag="ACFI8B_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC3833538.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(15221..16285)
                     /gene="tsaD"
                     /locus_tag="ACFI8B_000225"
     CDS             complement(15221..16285)
                     /gene="tsaD"
                     /locus_tag="ACFI8B_000225"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC3833539.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(16401..17588)
                     /gene="ccsB"
                     /locus_tag="ACFI8B_000226"
     CDS             complement(16401..17588)
                     /gene="ccsB"
                     /locus_tag="ACFI8B_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC3833540.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(17581..19533)
                     /locus_tag="ACFI8B_000227"
     CDS             complement(17581..19533)
                     /locus_tag="ACFI8B_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC3833541.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKGTGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(19811..20434)
                     /locus_tag="ACFI8B_000228"
     CDS             complement(19811..20434)
                     /locus_tag="ACFI8B_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC3833542.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            20641..21270
                     /gene="yihA"
                     /locus_tag="ACFI8B_000229"
     CDS             20641..21270
                     /gene="yihA"
                     /locus_tag="ACFI8B_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC3833543.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(21390..23786)
                     /locus_tag="ACFI8B_000230"
     CDS             complement(21390..23786)
                     /locus_tag="ACFI8B_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC3833544.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLPQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            23938..25053
                     /gene="pilM"
                     /locus_tag="ACFI8B_000231"
     CDS             23938..25053
                     /gene="pilM"
                     /locus_tag="ACFI8B_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC3833545.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSAEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            25056..25655
                     /locus_tag="ACFI8B_000232"
     CDS             25056..25655
                     /locus_tag="ACFI8B_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC3833546.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            25656..26303
                     /locus_tag="ACFI8B_000233"
     CDS             25656..26303
                     /locus_tag="ACFI8B_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC3833547.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            26321..26866
                     /locus_tag="ACFI8B_000234"
     CDS             26321..26866
                     /locus_tag="ACFI8B_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC3833548.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            26885..29080
                     /gene="pilQ"
                     /locus_tag="ACFI8B_000235"
     CDS             26885..29080
                     /gene="pilQ"
                     /locus_tag="ACFI8B_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC3833549.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFDWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
     gene            30348..30860
                     /locus_tag="ACFI8B_000236"
     CDS             30348..30860
                     /locus_tag="ACFI8B_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC3833550.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            30940..32019
                     /gene="aroB"
                     /locus_tag="ACFI8B_000237"
     CDS             30940..32019
                     /gene="aroB"
                     /locus_tag="ACFI8B_000237"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC3833551.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
     gene            32758..33600
                     /locus_tag="ACFI8B_000238"
     CDS             32758..33600
                     /locus_tag="ACFI8B_000238"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC3833552.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            33629..34093
                     /locus_tag="ACFI8B_000239"
     CDS             33629..34093
                     /locus_tag="ACFI8B_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC3833553.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            34125..35234
                     /gene="ribD"
                     /locus_tag="ACFI8B_000240"
     CDS             34125..35234
                     /gene="ribD"
                     /locus_tag="ACFI8B_000240"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC3833554.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTLIATLERNEDRLRPYRKHAHVRILMPSETAGGK
                     IDLHRLMRLLAGEGYGEVMVEAGAKLTSAFLAENLADEIVLYRSPKILGIGSGLFCLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNGNAF"
     gene            35429..36850
                     /locus_tag="ACFI8B_000241"
     CDS             35429..36850
                     /locus_tag="ACFI8B_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC3833555.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTTVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIP
                     LALSSLAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLLQSIFSTVWTPYIFR
                     AIEENATPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFTVVSCMLPP
                     LFYTLTEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGTRGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKNLHKLFHYLKKQGFPL"
     gene            37018..38259
                     /locus_tag="ACFI8B_000242"
     CDS             37018..38259
                     /locus_tag="ACFI8B_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC3833556.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSAIIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            38390..39565
                     /locus_tag="ACFI8B_000243"
     CDS             38390..39565
                     /locus_tag="ACFI8B_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC3833557.1"
                     /translation="MLNTALSPWPSFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            39613..41523
                     /locus_tag="ACFI8B_000244"
     CDS             39613..41523
                     /locus_tag="ACFI8B_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFC3833558.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVTTVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVKITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            41579..42871
                     /locus_tag="ACFI8B_000245"
     CDS             41579..42871
                     /locus_tag="ACFI8B_000245"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC3833559.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAIYCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(42923..43759)
                     /locus_tag="ACFI8B_000246"
     CDS             complement(42923..43759)
                     /locus_tag="ACFI8B_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC3833560.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(44054..44257)
                     /locus_tag="ACFI8B_000247"
     CDS             complement(44054..44257)
                     /locus_tag="ACFI8B_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC3833561.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(44235..44468)
                     /locus_tag="ACFI8B_000248"
     CDS             complement(44235..44468)
                     /locus_tag="ACFI8B_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC3833562.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     GTEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            complement(44480..44716)
                     /locus_tag="ACFI8B_000249"
     CDS             complement(44480..44716)
                     /locus_tag="ACFI8B_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833563.1"
                     /translation="MPIAGVSGSPRLRMCISVNADSSGNRICSLPLGRISLDGWRHTG
                     FQTAFLRNRQCGHIAGFAAILGAAAAGATITPSF"
     gene            45001..48435
                     /gene="dnaE"
                     /locus_tag="ACFI8B_000250"
     CDS             45001..48435
                     /gene="dnaE"
                     /locus_tag="ACFI8B_000250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC3833564.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            48477..48731
                     /locus_tag="ACFI8B_000251"
                     /pseudo
     CDS             48477..48731
                     /locus_tag="ACFI8B_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(49087..50017)
                     /locus_tag="ACFI8B_000252"
                     /pseudo
     CDS             complement(49087..50017)
                     /locus_tag="ACFI8B_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(50087..50630)
                     /locus_tag="ACFI8B_000253"
                     /pseudo
     CDS             complement(50087..50630)
                     /locus_tag="ACFI8B_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(50576..50791)
                     /locus_tag="ACFI8B_000254"
     CDS             complement(50576..50791)
                     /locus_tag="ACFI8B_000254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833565.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTDSIPASPPAA"
     gene            50951..51610
                     /locus_tag="ACFI8B_000255"
     CDS             50951..51610
                     /locus_tag="ACFI8B_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC3833566.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
BASE COUNT        12399 a        15363 c        12937 g        10967 t
ORIGIN      
        1 tgtctccaaa cggaaaaaag cggtttgttt tttgttgtta acatttttat tgtaattaaa
       61 taaaaggtcg tctgaaaacg gttttcagac gaccttttgc tataatcggg cttcatcgcc
      121 ccgttcggtt tggaacctta tgaaaaccct cgtcctcctc ctgctttcct tctccacgac
      181 caccgctttc gccgcatacg gtttgggttt ggggcaggca ccgaaatatc ctgccggctt
      241 tcgcgcctac ggttatgttt attccggacg gcagggctgg gttttaaaaa cagaggcgga
      301 tgccattaaa ttagacacgc ttttcaaacg ctttgtgtac cgtccttccg ccgccaatca
      361 aaaccccgtc ggacagcgtt cggacggcat acccgccaac cacacaaagg aaaaaccatg
      421 agcaaaaaaa tcaaagccgg cattgtcggc gcgacgggct acacgggcgt ggaactgctg
      481 cgcctgcttg ccgcccatcc agatgtcgaa gtcgccgccg taaccagccg cagcgaagcg
      541 ggaaccgcag ttgccgatta ctttccaagt ctgcgcggcg tgtacggcct cgccttccaa
      601 acgcccgacg aggcaggttt ggaacaatgc gacatcgtct tcttcgccac gcccaacggc
      661 atcgccatga aagacgcacc gcgcctgatt gaacagggcg tgcgcgtcat cgacctttcc
      721 gccgacttcc gcatacggga cattccgacc tgggaacact ggtacggcat gacccacgcc
      781 gcccccggcc tcgtttccca agccgtgtac ggattgagcg aactcaaccg cgaagccgtc
      841 gcacaggcgc gcctcgtcgc caacccgggc tgctacccga cctgcgtatc cctgccgctc
      901 gtgccgctgt tgcggcaatg ccgtctgaag cccggtatgc cgctgattgc cgactgcaaa
      961 tccggcgtgt ccggcgcggg caggaaaggc aatgtcggtt cgctgttgtg cgaagccggc
     1021 gacaacttca aagcctacgg cacagccgga caccgccacc tgcccgaaat caggcagacc
     1081 atcgccgggc ttcaggacgg catcgccgaa ggattcgtgt tcacgccgca cctcgcgccg
     1141 atgatacgcg gtatgcacgc caccgtttac ctccaccttt cagacggcag cgaccccgaa
     1201 accgtcctgc gcgactacta ccgcgacagc ccgttcatgg acatcctgcc cgccggttcg
     1261 acacccgaaa cccgcagcgt gcgcggcgca aacctctgcc gcatcagcat ccgtcaggcg
     1321 gcgcaatccg atgtgtgggt cgtcctttcc gtcatcgaca acctcgtcaa aggcgcggcg
     1381 ggtcaggcag tccaaaatat gaacattatg ttcggactgg aggaaacaca cggcttgggc
     1441 ggcatccccc tgctcccctg aagcgcaaac ggcaaaccgc aggcatcgtg cctgcggttt
     1501 tttgatgccg tctgaaagcg gcggtttttt tgggttcgga cggcttttgg ctcatcattc
     1561 cgcgaaaaca aaaatctaaa ataccgtcat tcccgcaaaa gcgggaatct agtttatccg
     1621 gcttcagcaa tttccgacac atttccacac gcttcgattc cgtcatttct ccggtttcaa
     1681 tcgtttccga taacaccgtg gtttttcatt tctagattcc cgcctgcgcg ggaatgacgg
     1741 cggggggctt gccgttttcc cgataagcct ctgcggcttc tcattgccgg atttccgccc
     1801 acttgggaat aacgggattt aaaatcatga aaatgtgtca aaaataatat agtggattaa
     1861 caaaaaccgg tacggcgttg ccccgcctta gctcaaagag aacgattctc taaggtgctg
     1921 aagcaccaag tgaatcggtt ccgtactatt tgtactgcct gcggcttcgc cgccttgtcc
     1981 tgatttttgt taatccacta taaaaaatca gatttccgtt acactttttt ctaatatttc
     2041 agacggcatt ttgctcacac acccaaataa ccttccctgc cggaaagcca ccttgccaaa
     2101 tgcgcttcga cgatttcagg gttttgttca atcagcatcg gggcggtttc gcgcgcttgt
     2161 tccaaaaggt gtaagtcttc ttcgagcttg gcgaagcgca gcataggcac gccgctttgg
     2221 cgcgcgccga gaaattcgcc ggggccgcgg atattgaggt cttggcgggc gatttcgaag
     2281 ccgtcggtgt gttcgtagat gactttcagc cgcgctttgg cgagttcgcc caagggttcg
     2341 gcaaacagga ggacgcacac gctttccccc gcgccgcgcc ctacccgtcc gcgtaattgg
     2401 tgaagctgcg ccaagcccat gcgctcggcg tgttcgatca ccatcagggc ggcgttgggc
     2461 acatctacgc cgacttcgat gacggtggtg gcaaccaaga cgttcagacg gcctgcggca
     2521 aattccgcca tgacttcggc cttttcggcg gccttcatgc gcccgtgtac cagtccgata
     2581 ttgggttcgg gcaatgccgt ctgaagccgg gcgagggttt cggcggcggt ttgcagttgc
     2641 agggtttcgc tttcttcaat caatgggcag acccaatatg cctgccgccc ttttcggcaa
     2701 gtgccgagga cgaagccttc gacttcggcg cggcggacgt tgttgacgag gcgcgtttta
     2761 atcggcgtgc gcccgggcgg caattcgtcg atgacggata cgtccaagtc agcgaagaag
     2821 ctcatggcga gcgtgcgcgg gatgggtgtg gcggacatca tcagctgatg gacttcgcgc
     2881 cctttgtttt tgagtgcgag gcgttgtgca acgccgaaac ggtgctgttc gtccacaatg
     2941 ctcaagccca aattgtgaaa cgccacgccg tctgaaaaca gggcgtgcgt gccgacggcg
     3001 attttgacgc tgccgtcggc gagtttggct ttggcttcgt ctttggcttt tttgcgcaaa
     3061 ctgccaaaaa ggcagacaac ttcaatgccc aaaggttcga gccattgttt gaacttgatg
     3121 aagtgctgtt cggcaaggat ttcagtgggc gccattacag ccacttgcgc gcctgattcg
     3181 atggcggtca gcgcggataa ggcggcgacg atggttttgc cgctgccgac atcgccttgc
     3241 agcaggcggt gcatggggtg ggtttgcgcc atatcgcggc agatttcgga aacaactttt
     3301 tcttgtgcat cggtcagggc aaacggcagg gcttggcgca gggcttgggt caatgtgccg
     3361 tcgccgcgca atgccgccgc cgtgccgctg acgcgcttct gccgcgccaa acgcatggac
     3421 aactgttgcg ccaaaagttc gtcgaatttg agccgctgcc acgccggcag cgtgccgtct
     3481 gaaagttgat gaatagtgaa actcggcggc ggcgaatgca aaaggcgcag gctttcggca
     3541 aggcgcggca gcttcagacg gcctaataaa gcatcaggca aggtgtcgtg cagcggcgta
     3601 acgtccaacg ccgtctgaat gatgcggcgc aaagtgggct ggttcaaacc gtttacggtc
     3661 gggtaaaccg gcgtgaggct ttccgccaaa ccgccgccct cggcatcgcg gattttggga
     3721 tgaatcatct cgtcgccgta aaatccgtgt ttgatttcgc ccacggcgcg gatgcgtttg
     3781 ccgaccgccg tctgcttctg atggctggcg taaaagtgga tgaagcgcag aaaaaggacg
     3841 ctgccggaac cgtcggcaat ttggacaatc agctgcttgc gcggtttgaa cgttacttcc
     3901 tgatggataa cctcgccctc gacctgacac ggcacgccaa tcggcgcgtc cttaatcggc
     3961 ataatgtgcg tctcgtcctc gtaacgcagc ggcaggtgca acaccaaatc ccacgcggta
     4021 tggaggttga gtttgtcgag cttcttggcg gaaacatcgg tgattttgag ctgtttctgg
     4081 gtttcgggcg acatcatagg cagattcctt tggacgcgcc tattttatcc gaaaacaaaa
     4141 atgccgtctg aaacggattc agacggcatc ggcaggcagg aatcaagccc cggcggcttc
     4201 ggcttcctgc tgttgttggt aaatcgcctc aaagttaatc ggcgcgagca ggacgggcgg
     4261 gaagccggcg cgcgttaccg taccggaaac cgcttcgcgc gcgtaaggga agaggatgtt
     4321 cggacacgcc acgcccaaca gcagctgcac atcttcttcg gggatgtttt ccagacggaa
     4381 aataccgctt tgggttactt cgttcaaaaa catcgtgcgc tcgttatcca atttggcggt
     4441 aacggttacg gtaacgtcca cgtcgtagta gccgtcttcc agcttttgac tgccggtgga
     4501 aacgcgcata tccacttcgg gatcgccctg ttccaaaaag atttgcggcg cgtgcggcac
     4561 ttccaaagac aagtctttga catacagtcg ctcgatgctg aatacgggtt gcagttcttc
     4621 gctcattttg ttttcctagt tgtgggttaa gggttcagca gtccgtccag cccgccttcc
     4681 tgctgaaggc ggtagaggtc ggtaaatcct ccgacgtgcg tttcgccgat gaaaatctgc
     4741 ggcacgctgc gctgtcccga aagccgctgc atttcggcaa aggcttcggg gcttgcatcg
     4801 acacggattt cgtcgatatg tccgacacct gccgcgtgca gcagcctttt cgccatcgcg
     4861 cagtaggggc aaaacggacc tgtgtacatg gtaacggtct gcatattggg tttccgaaag
     4921 ttttgcaatg ataatcaata taggggcaat cgctccgttt ggcaagtgcg gaacagatgc
     4981 acgttcagac ggcatgtgcg gagtgtgtca aagtttcttt tttaaagtat gataggcatt
     5041 gtgaaaaata tttttgcacc cgctctgcgc ggcggggcgg aacggatgca aaaatatttt
     5101 tattacattt tcaggaaaaa ccatgttgtc aggactcccc atccccaaag acatcgcgcg
     5161 cccgcccgaa acgatattgg tcaacatcac gccgcaggaa acgcgcgtgg cggtgttgga
     5221 ggaaaacaat atctgcgagc tgcacatcga gcgcaacagc gggcacagcc tggtcggcaa
     5281 tatctatctg ggcgtggtgc gccgcgtgct gcccgggatg cagagcgcgt ttatcgacat
     5341 cggcttggaa cgcgcggcat ttttacacat cgtcgatgtc ctcgaacaac gccgcaaccc
     5401 cgaagaaacc cagcgcatcg aacatatgct gtttgaaggg cagtcggttt tggtgcaggt
     5461 catcaaagac ccgatcaaca ccaaaggcgc gcggctttcc acccaaatct cgctggcggg
     5521 gcgtttcctc gtccaccttc cgcaagaaga ccacatcggc gtgtcccaac gcatcgaaga
     5581 cgatgccgaa cgcagcagcc tgcgcgaacg cctcgacaag ctcctgccgg aaaatgcctg
     5641 ccggggctac atcatccgca ccaacgccga aaacgccacc gacgaacagc tccagtccga
     5701 catcgactac ctgaccaaag tgtgggaaca catccaagaa caggcgaaaa tccggccgcc
     5761 ggaaaccctg ctttatcagg atttgccttt aagcctgcgc gtgttgcgcg atatggtcgg
     5821 ctgcgacacg cagaaaatcc tcgtcgattc caccgtaaac cacgggcgca tgacgcgttt
     5881 tgccgaacaa tacgtccacg gcgcattggg caggatagaa ttgttcaaag gcgaacgccc
     5941 gctgtttgaa acccacaaca tcgaacagga aatcagccgc gccctgcaac cgcgcgtcaa
     6001 cctcaacttc ggcagctacc tgattatcga gtccaccgaa gccatgacca ccatcgatgt
     6061 caacaccggc ggcttcgtcg gcgcgcgcaa tttcgacgaa accatcttcc gcaccaacct
     6121 cgaagcctgc cacaccatcg cccgcgaatt aaggctgcgc aaccttggcg gcatcatcat
     6181 catcgacttc atcgatatgg cgcaggaaag ccaccgcgaa gccgtgttgc aggagcttgc
     6241 caaagccctc gccttcgacc gtacccgcgt taccctgcac ggttttacca gcctggggct
     6301 ggtcgagctg acgcgcaaac gctcgcgcga aaacttaaac caagtcctct gcgaaccctg
     6361 cccttcctgc caaggcaggg gccgcctgaa aacgccgcaa accgtgtgct acgaaatcca
     6421 gcgcgaaatc gtccgcgaag cgcgccgtta cgatgccgaa agtttccgca tcctcgccgc
     6481 ccccaacgtc atcgatttgt ttttggacga agaatcgcaa tccttggcaa tgctgataga
     6541 tttcatcggc aaaccgattt ctttggcggt cgaaaccgct tacacgcagg aacagtacga
     6601 tattgtgctg atgtaaaaaa tgccgtctga agccttcaga cggcatctgt ctatttcagg
     6661 gtttccttgt ccaacagcgc gcgtatcaac agcccgtgtc cgaaacgtcg gctgtcggtc
     6721 agttccaaat atccgccgta ttttttggcc agcgtgtcgg caatcgacag ccccagcccc
     6781 gccccctgct gctccgttcc caaaatacgg taaaacggat cgaggacgcg ggcgcattcg
     6841 gattcgggaa tgccgttccc gttatcctcc acccacacgg cgagatattt cccttcgtcc
     6901 gtgaaaccca aatctatcct gcccccgttc ggcgtgtaac gtaccgcgtt gtcggcaaag
     6961 gttttaacca gcgtatagat ttccgtttcg tcggcagaca cttcgacatc gcctccgacc
     7021 gccacaccga tgtcctgacg tttttccaaa accagcggca tcagttcctg caacacttgg
     7081 cggaaacggc tttgcagacc gaatgtcgtt ttcgccaagg ggggtttcgt ccgactgcga
     7141 acgcgccagt gccaaaagct gttcgagcag gtgtttgttg cgcctgatgc tctgctgcaa
     7201 aacggcggac tgccgccccg catcgggtgg gagcggcata ttgttgagcc gttccgcctg
     7261 aagggaaagg gcagtcatcg gcgtacgcaa ttcgtgtgcc gcgtcggcga caaaccgctg
     7321 acggcggcgt atatcttcat caacacgttt caaaagcaga ttgatggcgg ttacgaaccc
     7381 tctgatttca ctgggaatat tgtacacact caaagcaggc aggccgttga ttcggcgttg
     7441 ttcgagactt tgcgacaatt tgcggacggg gcgcatggct ttgtgcgtaa tccacacagt
     7501 cagcagtatc atcagcggca gtgccgccaa caggggcaac acgctttgcc gtgccgcatc
     7561 ctccgccaaa tcttcacggt attcgttttc ctgcatgacg gcaatccgtc cctgttcggt
     7621 cgtgcggata taggcgcggt aataatcgtc gtccccgtcc gcccgaagcg tgtgcagacc
     7681 gtccgccaga tgcgcgggca ggctgacaac agggtcttcc tgctgcggca tctgtaccaa
     7741 aatacgcgta tcgccgtcgc cttcgggcaa agtttcggat ttggaatcag ggccgacata
     7801 caatgccgcc tgacggagca ggtcgtcctg caacgcttcc gtttcgtgga aggtttcgta
     7861 gtaggaaaac atacctgcaa gcattgccag cggaacaaac atccaaatca gggcaaggct
     7921 gattctgacc tgaagcgaat gttttaaaat tttaaaaaaa cggtccggca tcttcgttcc
     7981 tgttcagacg gcatcttgac gcggcatcag ccagccgaca ccgcgcacat tttgtatgct
     8041 ttccttaccc aatttcttgc acagcccgtg aatcagaaaa tccaccgcat tgctttcgac
     8101 ttccccgccc caaccgtaaa ccttgtcctc cgaatccgag cgcgacaaaa tcacacccgg
     8161 cctcgccagc aaagcctgca ataccgaaaa ctcctggttg ctcaatgcca cctgcctccc
     8221 ctcggcaata atttctacct gatacgttgc aggattgagg gataacgcac catttgacag
     8281 acacgcctgc gcctgtccgc tcccgcgccg caacaccgcc cgcatccgcg ccttaaactc
     8341 cgccatatcg aacggcttga cgatatagtc atccgcaccg ccgtccaaac cgttcaaccg
     8401 gctgtacaaa tcatcccgcg ccgtaacgat cagcacgggg acggtacagc ctgccgcgcg
     8461 tatttccgac aaaacatcca aaccgtcccg cccaggcaaa cccaaatcca gcagcatcaa
     8521 gtcataaggc tgggcagcgg cagccgccgc aacctgcgcg ccgtttttga cccaatccac
     8581 cgcatagccg ccgtctttca aacttgccga caccgcttac gcaatcatcg catcgtcttc
     8641 caccagcaaa acacgcatca actttccctt caaaataaac cgtgcctatt ctaacacccc
     8701 aaaattagcc gcaatttagc ggcctttacg cttgccggta tttttcaaaa ctgcggcaca
     8761 aaaaaaccgc gccggcaact gccttcagac ggcattgggg cgcgattgca acacacggac
     8821 aggcaggcaa agcctgcgac agatcacagg aacgattcgg gcttcaggcg gcttcgccgt
     8881 ttacggcaga ggcacgattc ctgccgctat cgaactgacc aatatcgcca gcgacaaacc
     8941 ccacgcccag aaaaacgaat aacggatgtg tttgcccatc gacaatttcg ccaaacccaa
     9001 acccatccac aaagccggcg aaagcggcgt aacaaaagta ccgacgatac tgccgatcaa
     9061 catcgcatag cctgccgctt cgggtgcaac gcccgcctgc gaggtaatct gttccacaat
     9121 cggaaacagt ccgaaataat aagcgtccgt actcaaaacc aactcaagcg gaatacccaa
     9181 cacaccgatg gcaatatgca gataaggcag caacgcgtcc ggcaggatat gcacaaggtc
     9241 tttggaaatc gcgtccaaca tccccgcgcc cttcaaaatc cccaaaaacg tacctgccgc
     9301 caaaataatg gacgccatca tcaccgcgcc gccggcgtgg gcataaatcc gctccatctg
     9361 ttcctgcggg ctgcggtaat tcaaaagcaa cgccgccgtt gcagccagca taaatacata
     9421 acccggcggg aagatgcccg aaaaaagcag gctcatcgcc gccaaaaaca gcaggacatt
     9481 ccaccaaaac agtttcggac gcgccaattt ttgttcttct tccgacaaag gcgccggctt
     9541 tatcaaatcc gccacggcgg gcaacgcgcc caactcccgg acaatccgcc ttttttcacg
     9601 cacacccaaa aacagggaca gcacaaggat aaacaccaca ccgataattt gcaccgtcaa
     9661 caaaggttta tacaattcgc ccacatctgc gcccaacacg cttgcaaccc gcccgatcgg
     9721 cccgccccgc ggcaaaaggt tgattagccc cgcgctggaa gtcagcagca aaaacagcag
     9781 gtaaggattc atatgcagac gcttgtaaag cggcaaaagg gcggggacga ccgataaaaa
     9841 cgtcgtcgcg cccgccccgt ccaactgtgc caccaccgac accaagaccg tccccacact
     9901 cactgccacg atattacccc gagtcagctt aatcaaaccg cctatcatcg gacggaacag
     9961 ccccacatcg ttcatgattc caaaaaacaa aatggaaaac ataaacataa tcacaatctg
    10021 cgtcaccgat ttcgtgccgc ccgaataaaa ttcttttaat tgggatacat caaaccccgc
    10081 cagcaacgcc ccaatcagcg gcaccaagat taatgcgatg atgggcgaca ctttttccgt
    10141 cagcaacagc catacgatga ccccgataat cagcaatccg ataaacgtca gcattatttc
    10201 tctttatttt attttgaaca taaaaccgcc cgtgcaggca aaaccgccaa catatgcggg
    10261 ataagccttg cattccattc acgcctgccg acatttctgc atccgtccgt catttcacgg
    10321 cggcaaccga tacggaacaa ccggtatcgg gacggcgcgg gggcatccgt cccggtgcgc
    10381 cgattcaaac gaaactgccc cctatcattg cggagcgcgg ggcgtgccgt acacgcggga
    10441 ttttaaacac gccgatcatt tgccgcgcat tatccggcaa acggcaaacc ttgacgctgc
    10501 ccggtccata taaaaaagcc gcaaaacccg aaccggtttt gcggcttttc gatttggttt
    10561 gtttatttgg catttctttc aatcaaaccg acaaactgat tgatatatga ctgaacaaaa
    10621 tcccttgccg agtcaatcaa cttaccgttt tcatcaaaca gcgtcggcga attgcccaaa
    10681 aatacttccg gctgtccggt tacgggcata tcaaaatacg acagggcaag gcgcaggttt
    10741 ttttgggaac tgtaaccgcc catcttgccg acggaatggc tgatgatgcc ggccggtttg
    10801 tttttccaag ccacgtcggc attcggtttc gagccgatgt ccaccgcatt tttcaaacag
    10861 gcgggaatgg tgcggttgtt ttcggacgta acgaacaaaa tgccgtccga agccttaatc
    10921 gtttcgcgga aagccgtgta gctttcgggc agcggcacat cttccaccgc agggtcgtca
    10981 taatcgaaat tgtaaagcgg cagatgtccg atttcgacga tttccgcctg ccagccttcg
    11041 gggaacatct ccgccgcatt caatgccact ttgcgcgcaa acgaagcacg gcgcaggctg
    11101 cccaccaaaa tactgatttt cttagccaca atcattcctc ctgaatatta agtttgtgcg
    11161 tttcaatcat ttcataatgg taacgattat tatatatgtg atttcccctg caaacaagcc
    11221 ggtccgccgc cccggcgttc ccacttatcc ggctttgcct tataattgct ttttttatgt
    11281 aacagattca cctatgaatt tccccaaaac agcggcctcc ctgctgctgc ttctcgcctc
    11341 cctcgccgca cacgcgctcg ataccggccg cattccgcaa aacgaaatcg ctgtatatgt
    11401 ccaagagctt gacagcggaa aagtcatcat tgaccaccgt gccggcatac ccgtcaatcc
    11461 cgcgtccacg atgaagctcg ttaccgcgtt tgccgccttc aaaaccttcg gcagcaatta
    11521 ccgctgggcg accgagttta aaagcaacgg tacggtaaac gacggcacgc ttgacggaaa
    11581 cctgtattgg gcgggcagcg gcgaccccgt tttcaatcag gaaaacctgc ttgccgtcca
    11641 acgccagttg cgcgacaaag gcatccgcaa tatcacgggg cgcctgatgc tcgaccacag
    11701 cctgtggggc gaagtcggca gtcccgacca ttttgaagcc gacagcggtt cgccgtttat
    11761 gacgccccca aatccgacta tgctgtctgc cggtatggtt atggtgcgcg ccgaacgcaa
    11821 tgccgccggc agtaccgaca tcctcaccga tccgcctttg ccgcatattt ttgcccaaaa
    11881 caacttgaaa attaccgcct cccaagctgc ctgcccttcg gtcaaaaaac tgatgcgcgc
    11941 atctttttcg ggcaatacgc tgaaattgcg cggcaatatt cccgaaagct gtttgggcaa
    12001 gcctgtcggt gtccggatgt tcgcgcttga cgaactgatc cgccaaagtt ttaccaaccg
    12061 ctggctgctc ggcggcggac ggatttcaga cggcatcggc atagccgaca caccggaagg
    12121 cgcgcagacg cttgccgttg cacactccaa accgatgaag gaaattttga cggacatgaa
    12181 caagcgttcg gacaatctga ttgcgcgttc cgtcttcctc aaactcggcg gcgacggcaa
    12241 actgcccgcc gtttccgaac aggcggcgtc tgccgtccgg cgagaacttg ccgtatcggg
    12301 catcgatgtt gcggatttgg ttttggaaaa cggttcgggt ctgtccagaa aagaaagggt
    12361 aacggcgaga atgatggcgc aaatgttgga aacggcttat ttcagcccgt ttgcacaaga
    12421 tttcatcgac acgctgccca tcgccggcac agacgggact ttacgcaacc gcttcaaaca
    12481 aagcggcggg ctgttgcgct taaaaaccgg cacgctcaac aatgtccgcg cccttgcagg
    12541 ttattggctg ggcgacaaac cgatggcggt ggtcgtcatc atcaacagcg gccgcgccgt
    12601 ttccctgctg cccgacttgg acaacttcgt tgccaaaaac atcatctccg gcggcgacgg
    12661 ctggctggat gcgaaactga tgtgcaaaga acgccgcgcc tgaatcggga aaatataaaa
    12721 atgccgcctg aactgttcgg acggcatttt tgattttcaa accggaatta cagccccgct
    12781 gccgctttca atgaggccgc tttgtcggtg cgctcccaag taaactcggg ttcttcgcgg
    12841 ccgaaatgtc cgtaggcggc agattttccg taaatcgggc gcaagaggtc gagcatttgg
    12901 acgatgcctt tgggacgcag gtcgaaatgt tcgcaaacca gcgcaatcag tttttcctcg
    12961 ctgattttac ccgtaccgaa agtatcgatg gaaatcgaag tcggttcggc aacgccgatg
    13021 gcgtaggaaa cttggatttg gcattgggtt gccaagcctg cggcgacgat gtttttcgcc
    13081 acataacggc aggcgtaggc ggcggaacgg tccactttgg acgggtcttt gccggagaat
    13141 gcgccgccgc cgtgcggagc cgcgccgccg taggtatcga caatgatttt gcggccggtc
    13201 aaaccgcagt cgccttgcgg gccgccgatg acgaagcggc cggtcgggtt gatcaggtat
    13261 tttgtttcgt cggtcagcag ttcgggcggc aaaacgggct tgataatctg ctcaatcacg
    13321 gctttactca gttcttcatg gctgatggca ggatcgtgct gggtggacag gacgacggta
    13381 tcaatgcgtt tcaccttgcc ggtttcgctg tcgtaaacca cggtcagttg ggcttttgcg
    13441 tcagggcgca gccaaggcag gcggccgtct ttgcgcaatt cgctttgacg ctgcatcagg
    13501 cggtggctgt aatagatggc aaacggcatc agggtcgggg tttcgtcgca ggcgtagccg
    13561 aacatcaagc cttggtcgcc cgcgccttgg ttcaggtcga tgccttcgcc ttcgttcacg
    13621 ccttgggcga tgtcggggga ttgttggtcg tagtacacgc cgaccgcgca gccgttggcg
    13681 tcaaagccca gctcggagga gttgtagccg atgcgtttga tggtttcgcg tgcgactttg
    13741 atgtagtcca cttgggcggt ggtggtgatt tcgcccgcca atacgcacaa gcctgtgttg
    13801 accaaagttt ccgccgcgac acgcgctttg gggtcttgcg ccaagatggc atccaaaatc
    13861 gcatcggata cttggtcggc tactttgtcc ggatggcctt cggaaaccga ttcggaagta
    13921 aacagatatt cgctcattga aattttcctt gaaaaacaaa ccatcttctt cagacagcat
    13981 gttgtatgaa cataatgtcg acagcgggaa atatagcaaa atttccctat tcgtgccatt
    14041 cagttgagaa atattcctat ttaaacggcg ctttggaatc tctgtccgta cgtttcgtac
    14101 aggcaaaaaa attcccgcat caagcgggtt tggattgctc cggcgagcca catcggtttt
    14161 tcaaccgtcc accttacctt tccgtttgaa aagcaggttg gcatggaatt cccaactctt
    14221 aatgcagcgc gcatcgtagc agaaaaggca tattgccgca atacttccct ttttcagacg
    14281 gcatgtttcg tttacaattc aggctgtttc cccctttgcg aaccgccatg cacatcctgt
    14341 tgaccgccct gctcaaatgc ctctccctgc tgtcgctttc ctgtctgcac acgctgggaa
    14401 accggctcgg acatctggcg ttttaccttt taaaggaaga ccgcgcgcgc atcgtcgcca
    14461 atatgcggca ggcgggtttg aaccccgaca cgcagacggt caaagccgtt tttgcggaaa
    14521 cggcaaaatg cggtttggaa cttgcccccg cgtttttcaa aaaaccggaa gacatcgaaa
    14581 caatgttcaa agcggtacac ggctgggaac acgtgcagca ggctttggac aagggcgaag
    14641 ggctgctgtt catcacgccg cacatcggca gctacgattt gggcggacgc tacatcagcc
    14701 agcagcttcc gttccacctg accgccatgt acaagccgcc gaaaatcaaa gcgatagaca
    14761 aaatcatgca ggcgggcagg gtgcgcggca aaggcaaaac cgcgcccacc ggcatacaag
    14821 gggtcaaaca aatcatcaag gccctgcgcg cgggcgaggc aaccatcatc ctgcccgacc
    14881 acgtcccttc tccgcaggaa ggcggcggcg tgtgggcgga ttttttcggc aaacctgcat
    14941 acaccatgac actggcggca aaattggcac acgtcaaagg cgtgaaaacc ctgtttttct
    15001 gctgcgaacg cctgcccgac ggacaaggct tcgtgttgca catccgcccc gtccaagggg
    15061 aattgaacgg caacaaagcc cacgatgccg ccgtgttcaa ccgcaatacc gaatattgga
    15121 tacgccgttt tccgacgcag tatctgttta tgtacaaccg ctataaaacg ccgtaacgaa
    15181 aataaaaatg ccgtctgaac aggtttcaga cggcatcttg tcatttgacg atttcggaca
    15241 acgaccaacg cggacggacg ttgaacgcac cgacctccct gcccttgccc aggtgcatcg
    15301 caccggcaaa ggcaatcatg gcaccgttgt ccgtgcagta tgccatcggc gggaaaaaca
    15361 cgctgacttt ttcggacgga tgtttcggct tgcctttggg ggtcgggatt tgtaccgtca
    15421 tgttgccgaa agtttcacgg agtttgcggt ttgcaccgac cccgccggcg accactacgg
    15481 ttctgaaccc tgtctgcaac agggcttttt tcacttttgc ctccaacact tctactaccg
    15541 catcttgaaa cgcacggcaa atattgttgc gggtttgctc gggtatggtt tccgaaccgt
    15601 ttgcctcacg cactttttca acggcagtca aaacagcggt tttcaatccc gaaaaactca
    15661 tctgcaaatc gtcggaatga atcatcgggc gcggaaaaac aaacgcttcg ggcctgcccg
    15721 attccgcaag ttccgacagt ttcgcaccgc ccggatacgg caaacccagc agtttcgccg
    15781 ttttgtcgaa tgcctcgccc gccgcatcat cgacgctctc gcccaaaagc tcgtaatcgc
    15841 ctatgcccct gaccgccata atctgcgtat gcccgcccga aaccaacagc gcgacaaaag
    15901 gaaagtcggg tttttcctcc gccaacagcg gcgacagcag atgtccttcc aaatgatgga
    15961 cggggatgac gggcttatcc aacgctaaag ccagcgcgtt ggcgtagctc gaacccgcca
    16021 gcagcgcacc gcccaaaccg ggcccctgcg taaaggcaac cgcgtcaata tcgccatacg
    16081 atgcgcctgc ctgcgccaaa cagccttcag tcaacggaac aaggcggcgg atatggtcgc
    16141 ggctcgccaa ttccggcacg accccgccgt attccgcgtg cattgccatt tgagtgtgca
    16201 ggcagtgcga ccgcaatcct cgttcggtgt cgtacagcgc aacgccggtt tcgtcgcaag
    16261 aagattcgat tcctaatacc aacatggtct gatgccgtta aaaactgaaa aacgtattct
    16321 agcgaatttc ggcacgactg ccgtatcccc aaaacggaac atgccgtctg aagcccttca
    16381 gacggcatcg tcgcaccgta tcaaagcgtt ccgtaagaat gcagcccgct caaaaacata
    16441 ttcacgccga taaaggcgaa tgcggttacg aacaaaccga taatcgccca ccacgccagc
    16501 actttgccgc gccagcccgc caccagccgc aaatgcagcc acacggcgta attcaaccaa
    16561 acgatgaacg cccaagtctc tttcggatcc cagctccaat agcgtcccca agcgtccgcc
    16621 gcccacaacg cgcccaaaat ggtggcaatg gtaaagaaca aaaagccgac ggcaatcgcc
    16681 ttatacatca cctcgtcgat caatgccgac ggcggcagcc acagttttcc gcctttttct
    16741 tccgcacgca gggaaaccag ttcggcaata ccgagcatcg cggaaatgca gaacgcgccg
    16801 taaccgataa agtttgccgg aacgtggatt ttcatccacc aggactggag cgcgggaatc
    16861 agcggctgga tggtatgagc ctcacgcgac acgctgtacc acaacacaaa gccgaccacg
    16921 accgccataa agccgaacac gaagccgccc aatttctgca cggcaaattt accctcgtaa
    16981 tacaaataca tcagcgcggt aatgaccaaa aacagaatga acacttcata aagattggac
    17041 acggggatat gccctgcatc gggacggagc agatagcttt cgtgccaacg taccagcaga
    17101 ccggtaaagc ctgctacggc agacacccat gcaaacacgg ttcccatacc caacagcgtg
    17161 ttggtcggca cattttttac gcttgccaaa accgcgcccg aaatataggc gaacagggcg
    17221 aagaagacaa aagcacactg ccacatgatc gccgattggc tgctgaggaa ataccgcaac
    17281 aggaaaatct ctgccgattt aatgttgccg ccatacagac cgacggcggc ataggcaagc
    17341 aatacgctta aaggaacaaa ccagcgcatc ggtttgaaaa accaacccaa aaacacggca
    17401 ataccggcac tcgcccacaa catgaccgtt tcgtaaatgt ccatatgcat accggaacgg
    17461 gtctgcacga aaaccgtagc cgcaaaaacc agcacggcaa atacccaatc ccaaagattc
    17521 agattgcgga tcaaagattt ctgaatcagc agctcgtgtt ccggaagggt tttatagtgt
    17581 tcagtcatga ttcaagtcct tgccgagccg ttgcaggctc tcgacgtgtt ttggaaattc
    17641 cttctgcaaa tcccgttcgc tgcgggccga agacatagca aaacggattt tgccgtctga
    17701 aaacaatacc cacgcccgtt tttcgcgcac ataaaacata aataccgtac ccaaaaccaa
    17761 caataccgag ccgagataga ccaaaagcgc acccggcgaa cgggtcatct gcaaacctga
    17821 ggaacgcacc tcggaaaacc cgtcaagctg gagcagcata ggcgcgggat attccgtcag
    17881 ccccgtatag gcatccatac tgtgcagcag gaaacggttc cgcgcttcat cctgccgcca
    17941 ttcgggcaag ccgtaccggc gtatggtttc atccaaagca gcgttcatca cgccgtaaag
    18001 catttcgtag aaatagccct gcatcttatc ctgctgccct ttcgggatat tggacgtaat
    18061 aaattcgtcc aatcccaaat agcctttttg cgcaaagata ttcagcgtgt tttccgcagc
    18121 cagcatgaat tgttcgcgga tttcggcagg tgcgtctttg gttgcgtcgg caaccagacg
    18181 tttgcgccct tccccatctt tcaaaaactc acgcaatgcc ataaaggtgt ccgctttcaa
    18241 ctgcttgtcc aaggggatac gcagccagcg gtattgctgc tgcaagccgc tgcgcgtgcc
    18301 ggtcagccaa aaataatctt tgtcctgcaa aatcggcagc atatagtttt tatattcgac
    18361 cgcctgccct gccgcatcac ggatgcggta cacgatggaa gggccgatat tggtgtattt
    18421 tttaccttcc tgagtaacgg cgcggacatc gttcagagtg gatttcaggc ttttttcccg
    18481 ttccgcaccc tcgctcatgt cctccacatt catagaagtg aactgatcga actcaagacg
    18541 atatttgtgt ttgccgattt ccaacggaaa ctggtgtatg gaggttgcct tcaacacgac
    18601 aggttcgcgc gaagcatccc tcaaattcca cgccttgaat gtcaaatccg aaccgccgtc
    18661 ggcaaaactc gcctgataaa tcgtgatgcc gtgcaaggtc aaaggatggt tcacgcggat
    18721 ggtgcgctcg agtttctcac cggttccctt gtccgttact tcaatatcgc tggcaaaatc
    18781 gcgcggcata cccgtattgt aaaaatcgat atggaatttt ttcagtttga cttcaaaagg
    18841 caagtcctga accaacatcc cgttgtcggc attcaggaaa accacatccg cactttgccc
    18901 ctcggaaata ttgacgttgc ccctaaatga gagattggac gcacccaaaa tactttcggg
    18961 cttgaaatcc ttggcataaa ccgcctgatt gtccggaaca atccgaccgg ccagcatacc
    19021 cagcttcagc agcaggttac tgtctatcaa cccgcccagg caaatgacaa tcaaagcaac
    19081 atgggcaaag atatagcccc atttgttcat cgtgcctttt ttggcggcaa tcagaaccga
    19141 cccgtcctca cggctgacgg tttttccctg aaaaccccgc acctccagat aacgtttggc
    19201 aacttcgggg gcaattttta catccaacag cgaagaatgg cgcatcgccg ccagagattt
    19261 ttctttaacc ttttcccgga aagacttcat ttcgcgccaa aacggcggaa cgttacggat
    19321 taaacacaaa ctggtagaaa ccaccagaaa catcatgata acgacaaacc atgccgaagc
    19381 atagacatcg tacaaaccca aaaaatcaaa aatccgagtc caaaacggtc cgaatttgac
    19441 caaataatcc gtctgcggct ggttttgctg taacaccgtg ccgataaccg atgcaatacc
    19501 cagcagactg agcaaagcga ccgcaaagcg catggagctg aaaaaagcga accacggacg
    19561 ggaaagaagt gtgggggata tacgggattt actcattgtg tgtttattcc gccatcagga
    19621 atatgggaaa gcagaattgg gcagacagaa aacaacgtcc cgattctact gtcttgatgc
    19681 tttttatttc aagacaatga agacagcctg catcgattcc aacggttgcg attgaaaaaa
    19741 cttatcgcaa aattttcaga agccgtctga aaaccttttc agacggcttc tgaagcagac
    19801 ccttggatga ttaacgcaaa ccctggataa agttagcaac tgcttttaaa tcttcttcgg
    19861 acatacggtt tgcaatatct tccatgatgg tatttttacg ctgaccggac ttgtaggcat
    19921 tcatctgttc aacaatatat gcctggtgct ggccgcccaa acgcggataa gcctgaattt
    19981 cgcttccgcc tcccggcata cccgcaccgc tcggaccgtg gcaggacata cacgccggca
    20041 cttttttatc gctcaaaccg ccgcgataga ttttcgcgcc caattcggga ttttccttag
    20101 gattggcttc gccggatttg ggctgctgtt tggcatagaa tgcggatacg ttcaaaatat
    20161 cctgatcgct caaattcatt accaccggtt tcatcacagc tgccgaaccg tgggtgcgtt
    20221 tgccgtcgcg gatgccgata gtttgatgat agatgtaagc agtatgctgt gccgccaaac
    20281 gcggatacat cgcaatgccg ctgttaccgt ctgctgcatg gcaagccgca caaaccgttg
    20341 cggcaacctg tttgcctttt tccacgtctg ctttgggaga tgcggaaacc gcaccggcag
    20401 ccaaaacaaa ggccaataaa gtcaatcgtc tcatggagtg ctcctgatta cagcattgga
    20461 taacgcaaca atgctctttt ttatattcaa atatgggatt tttgacccga ttaaaaccga
    20521 tgattctgta aacgtgttat tctatactaa atttacatta aattaccact gtgtttcaca
    20581 taaaaccaac cgcatatttt tgctgtcgga caaacggcgg cggaaaacaa ggatatgccc
    20641 atgaaccttt ttcaaaacgc caaattcttc acgacggtaa accatctcaa agacctgccg
    20701 gacacaccgc tcgaaattgc ctttgtcggc aggagcaatg ccggaaaatc cagtgccatc
    20761 aataccctga ccaaccatgt ccgtcttgcc tacgtttcaa aaacaccggg acggacacag
    20821 catatcaact tcttcgagct gcagaacggc aattttatgg tcgatttgcc cggctacggt
    20881 tatgcccaag tccccgaagc ggtacgcgca cattgggtca atctgctcgg cgactacctc
    20941 cgccatcgga aacagcttat cgggctggtt ttgattatgg atgcccgcca tcctttaaaa
    21001 gaactcgaca tccgtatgct ggactttttc cacacgaccg gcagaccggt tcacatcctg
    21061 ctgtcaaaag ccgacaaatt atccaaaaac gaacagataa aaaccctgtc ccaagtcaaa
    21121 aaactgctca aaccttattc cgacaggcaa aacatcagcg tacagctgtt ttccagcctg
    21181 aaaaaacaag gtattgacga ggccaaccga actgtcggaa gctggtttga cgcagcagat
    21241 gccgccgctt cctctccaga ggaaaactga cctcaattat acggaaaccg tatcccccca
    21301 cttgaccggc cgcaaacatt taaaaaattg ccactgccaa atctaaaatg ccgtctgaaa
    21361 gacttttcag acggcatttt gcggagtctt taaaacaggg aatccaactg ctgctgtttg
    21421 gaatccgtat tgctcggaag caccggcgtt tcctgtacgt cctgacgcgt ttcgtcagac
    21481 cttccctgct gttcgttttc tactgccgct tcatcatctt cttttgcccg tcgggaaggt
    21541 tgcggcgcaa taccgctgtt gtccagcatc aagcccggat cggttaccat acgttccttc
    21601 atatagtatt cgccattgct gctgaccaca ccttcaggca ttttcatccc tttgccctgc
    21661 tttcctttca acgcaaaacg catatagtcc acccaaaccg gcaccgcaat cgtaccgccg
    21721 tagccggcac gccccatact cttaggtttg tcgaagccga tatatacggc agtaaccaca
    21781 tcagggttaa aaccgacaaa ccacgcatct ttattgtcgt tggtcgtacc cgttttaccg
    21841 gcaatatccg ttcttcccaa cgcagctgcc ccccttgccg taccgacacg gaccacatcc
    21901 tgcataatct tatacataat ataggcattg cgcggatcga ttgcctgagg cgcattttgc
    21961 cctgccacca aaggttgcat ttgggcgcgc aacctgccgt ctctgtcata aatcttatcg
    22021 atcacgtgcg aagaaaccct atatccgccg ttcgcaaata cactatatgc ctccgccact
    22081 ttcaacggcg tcgtctcgcc cgtacctaaa gccatagaca ggcttgccgg cagctcggac
    22141 ggcctgaagc cgaaacgccg gatatactgt tgcgcgtaac cgacaccgat agacatcaaa
    22201 atacggatgg aaaccatatt cttggaagcc gtcagagcct gtctcaaagt aatgtagccg
    22261 gaatatctgc cgtctgaatt tttaggtgtc caaaccgaac cgttcggccc tttccccggc
    22321 agggaaatcg gcgcatcgtt aaccactgtg gacgcggtca tccccttaga taatgccgcc
    22381 gaatagacaa acggcttaaa ggtcgaaccc ggctgccgca ttgcctgaac ggcacgattg
    22441 aatgttttgc tgtgaaaatc ataaccgccg accagcgcgc gcacagctcc ggtttttgca
    22501 tccagcgaaa ccaaagcccc ctgcggcaac ggctcttgaa ccaccgccca acgcccgccg
    22561 ttgtttttga cacggatgac cgcgcccctg cggatacggt cctcccccat tttctcatta
    22621 tcgaccgctc gggccgcaaa acccaaggcg cgcctgtcaa gcgcaacccg cctgccgccg
    22681 ggcagctgta tgacgacatt tttcttttta gtaacatcca acacaacggc gggaaccatt
    22741 ttatcgacgg tatagagtcc cgacagatac tggctgacag tctcctcgac atcttcactc
    22801 ttactcaaat cgatatagtt ttccgcaccg cggtagctgc tgccgcgatc gaaattccgt
    22861 agagccttgc gcaatgcctc ggttgccgcc ttctgatgat cggtgcggac cgtggtataa
    22921 accttaaaac cctgcgtata ggcatcttca ccatatttct catacagttc ccgacgcacc
    22981 atttccgcca catataaagc actctgatcg attttccgaa caaaccgctc ataatgcagt
    23041 tcctcattca atgcctgatc gcgctgttgc acggtaatca tcttctcctc gagcatattg
    23101 ttcaaaatat acttctggcg caacttggca cgctccggat taacaatcgg attataggca
    23161 gacggagcct tgggcagtcc cgcaagcatg gcggcttccg ccaaagtcaa atctcggaca
    23221 ttcttattga aatagatttg cgcggcagat gcaaaaccat aggcgcgctg accgaggtaa
    23281 atctgattga aatacaactc aaggatttta tctttgctta aagactgctc gattttatag
    23341 gcaagcaaca cctcattgaa tttgcgtgtg aacgtttttt cactgctcaa ataaaaattt
    23401 ttcgccacct gctgtgtaat cgtactcgca cccgactgca cgctgccgga cacgacattg
    23461 ccgacggcag cgcgggcaac accccaaaca tccacccccc aatgccggta aaagcgttta
    23521 tcctcggcgg caataaccgc attccgcaac acctcgggga aatcgccgat ttttgtaaat
    23581 tcgcgccgct gctccccata cataccgatg acttctccat ccgccgaata aatagtcaac
    23641 ggcattttag gctggtaatg ctgcaaagaa tccaaagacg gcagtttcgg atacgttacc
    23701 aaaatagcaa tggcaaccag acccactcca aatacacaaa aaccaaaaaa caaaccaaaa
    23761 caagtagtta aaatcttttt aatcatagct gaataataat ttaccattat tggtattaaa
    23821 taaagtaaaa tagcaaccga tttctacaaa gcacggtttc aatgtgcaaa gaacaaggaa
    23881 tccattacgg ataccgaaac ggttactcac tgtacaaata aagcaggaac tttcatcatg
    23941 cgcttgttta aaagcttgaa aaaccctaaa aaaacagatg ccaagctccc taaaaaatct
    24001 tcgggactca ataaccgcgc ggcaatcggc atcgatatcg accagcattc catcaaaatg
    24061 gtacaattgt caggacgtag tttaaaccaa attcaattgg aaaaatacgt cattgccaaa
    24121 ttaccaaaga atatcattca aggcaataaa gtccaaaatt acgatcaact tgttacatat
    24181 ttgcaacaag cctatgccaa actgggtact tcgtgcaaaa acatcgtcgc gtccgtcccg
    24241 caaaatctgg caaccatcga acaattgacc tacacggcca aagatgcaga attggacctg
    24301 caggggttcg tggagtcctc catctccgaa gccagctcga tatcgctcga agaagccaat
    24361 tacgactatc aggtcttgtc ccaatcggcc gtcggcgaag ctgttttgtc cgtcgcatcg
    24421 agaaaggatg aaatcgaacc cctgattgac gcattcaacg cagccggtat gaaattatcc
    24481 gcgcttgatg tggacatttt cggacaatac aacgcctacg cgctgtggat aaaccatttt
    24541 gcccccgagc ttgcggacga aaaagtggcc attttcggcg tatatgccgc acagacctac
    24601 gccctggtca tccaagacgg aaaaatccta tacaaacagg aaacctccgt cagcgcagaa
    24661 cagctcaacc aactcatcca gcgcacctat caggtaacag cagaaaaagc ggaagagatc
    24721 atcaactccc cgcaaaaacc ttccgattac caagaaagcg tggcaaacta tttcaaccag
    24781 cagattaccc aagaaataca aagggtcttg cagttttatt acaccacgca gaccgcagac
    24841 gatatgaccg acatcaagca tatcctgctg acgggggaag cggtgcgcca gaaaggcatc
    24901 gcccaaaccg tcgcctcgca aaccaatgcg gatgtacaat gcgtccatcc cgcgcgttat
    24961 tttgcgaacg acctcaaaac agacgaacaa caattcgaac ttgatgcgcc gacactgacc
    25021 aaggcgttcg gtttggcggt acggggatta taattatgaa caatttaatc aaaatcaacc
    25081 tcctccccta cagggaagag atgaacaaac gcaaacagca gcagtttaaa acgctgatgt
    25141 acggcgccgt gctgacgggc gttgccgccg ttgcggcaac ctacctgttt atcgacaata
    25201 tgatcaataa acagtcggaa aaaaacacgc tgctggaaat ctccatcgca cacttggata
    25261 ccgagctgtc ggaaatacaa aagctcaaac aggaaaaaga tgccttcctg attaagaaaa
    25321 acaaaatcgg ggagctccag atcaaacgcc tccaagccgc aaaaatcctc gacagcctga
    25381 atgaggccgt ccccggaagc acctacctaa cctcgctgga tgccgttacc gccgactctt
    25441 atcggctcag cggcaggaca tccagcgaca accgcgttgc cgccatgatg agggcgatgc
    25501 ccaataccgg catattcaag caacccgaat tgttaagcat caagaaaaac aattcgcatc
    25561 aagaatttac ccttcaggca acattacaac ctatcgtaaa ggcggccgaa tccaaagaga
    25621 atccggcttc gggaaacgca caggaggcaa actgaatggc ttctaaatca tctaaaacca
    25681 acttggatct caacaacctt cacctgctca accttcctgc caggcttttt atcgccctgc
    25741 tggtcgttgc cgccgtgctg gggctcggtt atgccggatt gttcaaaagc cagatggaat
    25801 cccttgagga atatgaagca aaagaaaccg aactgaaaaa cacctacaaa cagaaaagta
    25861 tcgacgcggc cagcctgaac aaccttaggg acgaacttgc ctcaatccgc tctgccttcg
    25921 atatcatgtt gaaacagctg ccgacagatg cagaaattcc caatttggtt caagagcttc
    25981 atcaggcggg ttcgagcaac ggtctgcgct tggacagcgt tatgccccaa cctcccgtag
    26041 atgacggtcc catcaaaaaa ttaccctatt ccatttccat taccggaaat tacgaacaga
    26101 tcagccaatt tacccgcgat gtcggcagtc tctcccgaat cattaccctt gagtcgctga
    26161 aaatcgccca atctccggaa aacggcggca atcctgacgg caagagcagt atcctgaacc
    26221 tcagcgccat tgccaccacc taccaagcaa aatccgtaga agagcttgcc gcagaagcgg
    26281 cacaaaatgc cgagcaaaaa taacttacgt tagggaaacc atgaaacact atgccttact
    26341 catcagcttt ctggctctct ccgcgtgttc ccaaagttct gaagacctaa acgaatggat
    26401 ggcacaaacg cgacgcgaag ccaaagcaga aatcatacct ttccaagcac ctaccctgcc
    26461 ggttgcgccg gtatacagcc cgccgcagct tacagggccg aacgcattcg acttccgccg
    26521 catggaaacc gccaaaaaag gggaaaatgc ccccgacacc aagcgtatta aagaaacgct
    26581 ggaaaaattc agtttggaaa atatgcgtta tgtcggcatt ttgaagtccg gacagaaagt
    26641 ctccggcttc atcgaggctg aaggttatgt ctacactgtc ggtgtcggca actatttggg
    26701 acaaaactac ggtagaatcg aaagcattac cgacgacagc atcatcctga acgagctgat
    26761 agaagacagc acgggcaact gggtttcccg taaagcagaa ctgctgttga attcttccga
    26821 caaaaacacc gaacaagcgg cagcacctgc cgcagaacaa aattaagaag aggattactc
    26881 cattatgaat accaaactga caaaaatcat ttccggtctc tttgtcgcaa ccgccgcctt
    26941 tcagacggca tcggcaggaa acattacaga catcaaagtt tcctccctgc ccaacaaaca
    27001 gaaaatcgtc aaagtcagct ttgacaaaga gattgtcaac ccgaccggct tcgtaacctc
    27061 ctcaccggcc cgcatcgcct tggactttga acaaaccggc atttccatgg atcaacaggt
    27121 actcgaatat gccgatcctc tgttgagcaa aatcagtgcc gcacaaaaca gcagccgtgc
    27181 gcgtctggtt ctgaatttga acaaaccggg ccaatacaat accgaagtac gcgggaacaa
    27241 agtttggata ttcattaacg aatcggacga taccgtgtcc gcccccgccc gcccagccgt
    27301 aaaagccgcg cctgccgcac cggcaaaaca acaggctgcc gcaccgttta ccgagtccgt
    27361 agtatccgta tccgcaccgt tcagcccggc aaaacaacag gcagcggcat cggcaaaaca
    27421 acaggcggcg acaccggcaa aacaaaccaa tatcgatttc cgcaaagacg gcaaaaatgc
    27481 cggcattatc gaattggcgg cattgggctt tgccgggcag cccgacatca gccaacagca
    27541 cgaccacatc atcgttacgc tgaaaaacca taccctgccg accgcgctcc aacgcagttt
    27601 ggatgtggca gacttcaaaa caccggttca aaaggttacg ctgaaacgcc tcaataacga
    27661 cacccagctg attatcacaa caaccggcaa ctgggaactc gtcaacaaat ccgccgcgcc
    27721 cggatacttt accttccaag tcctgccgaa aaaacaaaac ctcgagtcag gcggcgtgaa
    27781 caatgcgccc aaaaccttca caggccggaa aatctccctt gacttccaag atgtcgaaat
    27841 ccgcaccatc ctgcagattt tggcaaaaga atccgggatg aacattgttg ccagcgactc
    27901 cgtcaacggc aaaatgaccc tctccctcaa agacgtacct tgggatcagg ctttggattt
    27961 ggttatgcag gcgcgcaacc tcgatatgcg ccagcaaggg aacatcgtca acatcgcgcc
    28021 ccgcgacgag ctgcttgcca aagacaaagc cttcttacaa gcggaaaaag acattgccga
    28081 tctgggcgcg ctgtattccc aaaacttcca attgaaatac aaaaatgtgg aagaattccg
    28141 cagcatcctg cgtttggaca atgccgacac gaccggaaac cgcaacacgc ttgtcagcgg
    28201 caggggcagc gtgctgatcg atcccgccac caacaccctg attgttaccg atacccgcag
    28261 cgtcatcgaa aaattccgca aactgattga cgaattggac gtacccgcgc aacaagtgat
    28321 gattgaggcg cgtatcgtcg aagcggcaga cggcttctcg cgcgatttgg gcgttaagtt
    28381 cggcgcgaca ggcaggaaaa aactgaaaaa tgagacgagc gcattcgact ggggcgtgaa
    28441 ctccggcttc gggggcggcg ataaatggga ggcccaaacc aaaatcaacc tgccggttgc
    28501 cgctgccgca aacagcattt cgctggtgcg cgcgatttcc tccggcgcgt tgaatttgga
    28561 attgtccgca tccgagtcgc tttcaaaaac caaaacgctt gccaatccgc gcgtgctgac
    28621 ccaaaaccgc aaagaggcca aaatcgaatc cggttacgaa attcctttta ccgtaactac
    28681 agcctcgggc ggcggcaact ctaccaacac ggaactcaaa aaagccgtct tggggctgac
    28741 cgttacgccg aacatcacgc ccgacggaca aatcatcatg accgtcaaaa tcaacaaaga
    28801 ctcgcctgca caatgtgctt caggcaacaa cacaatccta tgtatttcga ccaaaagcct
    28861 gaatacgcag gctatggttg aaaacggcgg cactttgatt gtcggcggta tttatgaaga
    28921 aaacaacggc aatacgctga ccaaagtccc cctgttgggc gacatccccg ttatcggcaa
    28981 cctctttaaa acacgcggga aaaaaaccga ccgccgcgaa ctgctgattt tcattacccc
    29041 gaggattata gatacggccg gcaacagcct gcgctattga tgcgtcaaaa taagggcata
    29101 tgttttacag catatgccct ttctttatgc tttttgccgc gaccgaaatg ccgtcattcc
    29161 cgcgcaggcg ggaatccaac ttgtccggtt tcggttgttt ttcgtctcgt aacttttgag
    29221 ccgtcattcc cgcgaaatcg gaaatccagt ccgttcagtt tcggtcattt ccgataaatt
    29281 cctgttgctt ttcatttcta gattcccact tttgtgggaa tgacggcgga agggataaat
    29341 cctcgcaatc caaagcctcg tcatttccac aaaaaacagc aacccgaaac accccgtcat
    29401 tcccgagcag gcgggaatct aggtctttaa acttcggttt ttttccgata aattcttgcc
    29461 tcattgaaat tctagattcc cgctttcgcg ggaatgacgg gatatgggtt tccgtgcgga
    29521 cgtgttcgga tttccgcctg cgcgggaatg acgattcggg tattactgac gattcatcgg
    29581 gccgtagggt gggcttcagc ccaccaattt caccgcatca agggttttgg ggaataggcg
    29641 taactcggcg gtttcggttt ttggcgaagt tggttggaat cggcgggctg aagcacaccc
    29701 tacagtccac cctacaaccc atcttacgca tctgccttac gcatttatcc ttcgcctgcc
    29761 gatatgccgt cattcccgca caggcgggaa tctagaaccg caacgcggca ggaatctgtc
    29821 ggatacggct gaaaccgaac ggactggatt cccgcctgcg cgggaatgac ggctgcagat
    29881 gcccgacggt ctttatagcg gattaacaaa aatcaggaca aggcggcgaa gccgcagaca
    29941 gtacggatag tacggaaccg attcgcccgg tgcttcatca ccttagggaa tcgttctctt
    30001 tgagccgggg cggggcaacg ccgtaccggt ttttgttcat ccgctatagt attgataaac
    30061 atattatctt caatatattc aattggataa ttgtttacct aagcaaagat aattgccttt
    30121 tcctgacaaa ttaagtgaaa tcaacggatt gtcaaaacac agcctgaaat aaaaaacctc
    30181 cctgatttct tttatttttc cttaaaatca gaaaagttcg ggatggtcgg gttatttttc
    30241 caaacgtacc gccgccctgc cgatttcgta taaaatcccg ccgtaacccg acaagcccga
    30301 accctgccgc cccgaaaggc gggggggtca aacattaagg aaccgtgatg aaaaacttta
    30361 acggcaaact catcctcatc ggactgatgg gcgcgggcaa aactacgctg ggccggcaaa
    30421 tggcgcagcg gctggattac cgtttttacg acagcgatca cgaaatcgcc gcagcggcgg
    30481 gcgttcccat ccccaccata tttgaaatgg aaggcgaaca gggattccgt tcgcgcgaaa
    30541 ccgccatact caaaaaactg attgtcctgc cgcatatcgt cctgtccacc ggcggcggcg
    30601 cggtgttaaa agaagaaaac cgcgccctta tccgcaaaag cggcacggtc gtttatctgc
    30661 acgccccgcc cgaaaccctg ctcgaacgca cgcgctgcga caacagccgc cctttgctgc
    30721 aagttgccga tcctttggcg aaattgcgcg aactctacgc cgcacgcgac cccgtttacc
    30781 gccaaaccgc cgactttacc gtagaatcgg caaactgccg ggaaaccgtg caaaccctgc
    30841 tcaaacgctt atcccgataa accggcatat gcgccgctcc ccgaaaacca aaccgcaccc
    30901 cgcccggcgg gccggcggtt caaactttaa ggaacaacaa tgaaaacact gaccgtacac
    30961 acgccttcgc acagctaccc catctttatc ggcaacgggc tgctgccgca ggcgggaagc
    31021 ctgctcaaac cgcatttggg caaacgcgcc gccatcatca ccaacgaaac cgtcgccccg
    31081 ctctacctcg gcacgcttca gacggcattg gatgcggcag gcgtatccca tttcagcatc
    31141 atcctgcccg acggcgaggc gcacaaaaac tggcagacgc tcaacctcat ctttgacggg
    31201 ctgatgcaaa accgcgccga acgtaaaacc acgctgattg cgctgggcgg cggcgtgatc
    31261 ggcgacatgg tcggctttgc cgccgccacc taccagcgcg gcgcgccgtt catccaaata
    31321 ccgaccacgc tgttgagtca ggtcgactca tcggtgggcg gaaaaaccgc catcaaccac
    31381 ccgctcggca aaaatatgat cggcgcgttt taccagccgc aggcggtgct tgcagatttg
    31441 gacacgctgc acaccctgcc cgcccgcgaa ctgtccgccg gtatggcgga agtcatcaaa
    31501 tacggcgcgc tcggcgacat cggctttttt gaatggctgg aacagcatat gcccgaactg
    31561 atgacactcg aacgcgcgcc cctcacccaa gccgtgtacc gctgctgcca aatgaaggca
    31621 gacatcgtcg cccaagacga aaccgaacag ggcatacgcg catggctcaa cctcggacac
    31681 accttcggac acgccgtcga agcggaaatg ggttacggcg tatggctgca cggcgaggcc
    31741 gttgccgccg gctgcgtgtt ggcggcgcgt ttgtccgaac aactgggcaa aacctccgcc
    31801 gcagataccg cgcggctcgc cgccctgctc gaagccgccg gactgccgtc cgcaccgccc
    31861 gtgtttgcct ttgaaaaatg gctggcacat atgagccacg acaaaaaagt cagcggcggc
    31921 atcatgcgct ttatcggtct gaaccggctg ggcgaagccg tcatcaccga aattaccgac
    31981 acggacatcc tccggcgcac cctgcaaccg tatctctgat ttcctctgcc gatgtgttgc
    32041 cgcgcgggtt tgacgcacga tgatgacttt ccatcatcat tctccgcgaa agcgggcatc
    32101 cggttattcc atattgcagg gatttatccg gaataacgac aacctgtccg ccgtcatttc
    32161 cgcgcagatg gaaatttgga aattcaaaat cacaggaatt tatccggaat atagcggatt
    32221 aacaaaaatc aggacaaggc ggcgaagccg cagacagtac aaatggtacg gaaccggttc
    32281 gcccggtgct tgggcgcctt agggaaccgt tccctttgag ccggggcggg gcaacgccgt
    32341 accggttttt gttaatccgc aacagcaacc ctgtcgccgt cattaccgcg aaagcgggaa
    32401 tccagtccgt tcagtttcgg tcatttccga taaattcctg ttgcttttca tttctagatt
    32461 cccactttcg tgggaatgac ggcggaaggg ttttggtttt ttccgataaa ttcttgaggc
    32521 attgaaattc cagattcccg cctgcgcggg aatgacggct caaaagttac ggaacgaaaa
    32581 acaaccgaaa ccggacaagt cggattcccg cctgcgcggg aatgacggaa tcttaagttt
    32641 ccgtctttgt tttctgtttt cgcgggaata atggggaaca agccgtattt cagacggcat
    32701 tttcagttcg gggtataatc cgaatacttg cgaccatctg aatcatttgg acaaactatg
    32761 tgtcaactgc tgggcatgaa ctgcaatacg ccgaccgata ttatgttttc ctttgaaggc
    32821 ttccgccgca ggggcggcat taccgaccat cacgccgacg gtttcggcat cggctttttc
    32881 gaaggcaaag gcgtgcgcct gttccatgac gacaagccga gtgcaaattc ccccgtcgcc
    32941 gacctcgtgc gcgcctacca gattaaatcg gaaaacgtcg tcgcccacat ccgcaaagcc
    33001 tcgcaaggtc aaacctcgct ggcgaacacc catcccttta tgcgcgaaat gtggggcggc
    33061 tactggctgt ttgcccacaa cgggcatttg gtcgattttt tccccgaaca gggagaattt
    33121 ttccaccccg tcggcacaac cgattccgaa cgcgcgttct gccacatcct caaccgcctg
    33181 cgcacccgct ttgccgcccg tcccgacgac gacacgctgt ttgacgcgat tgcggggctg
    33241 acgcacgaaa tccgcaagtt cgggctgttt aacttcatgc tttcagacgg catttccctg
    33301 tttgcccacg ccagcacgct gctgcactac atcgtccgcc aagccccgtt cggcaaagcg
    33361 cgcctgctcg acgacgatgt aatggtcgat tttgccgaag taaccacgcc ggacgaccgc
    33421 gtcgccgtta tctccaccct gccgctgact cgcgatgaat cgtggtccca acttgccgtg
    33481 aacgaactgg tcatgttccg cgaaggcaac atcgtccggc acgaccgtcc cgaaaatccc
    33541 gtctatatga gtgccgaaga aggcctggaa atcgcccgcg ccgccggcgt atcggtttga
    33601 cgttcagacg gcatcaggaa gaaacaccat gaaatgcccg ttttgcagcc acccagacac
    33661 ccgagttgcc gattcccgcc tgatggaaga gcgcaatacc gtccgccgcc gccgccactg
    33721 ccccggctgc ggcaaacgct tcggcacgct cgaaaccgtc gaactcaaaa tgcccgccgt
    33781 catcggtccg gacaagaaac gttcgccctt caatgcacaa cgcctccgca acgacctgac
    33841 cgccgccgcc cgaaaatccg ccctgacacc cgaacagatt gacgaaaccg tccgcctgac
    33901 ggaacacagg ctctacactt cgggtcagcg cgacatatcc tcatccgaac ttgtcgaaat
    33961 cgtgctggag gagctgttcg ggcaaaacac ggaagccgcc gtccgtttcg ccgccctgca
    34021 caaacgcttc gacagcccgg aacatttcgc ctcgtggctg gcgcaagccg tcaaaacagg
    34081 cggcaaaacc tgattccccc aacccatact gatacggtat ccctatgttt tcggacacaa
    34141 atatatccat gatggaaaac gccctccgac ttgccgcttt ggggcgtttt tccacttcac
    34201 ccaacccgcg cgtcggctgc gttatcgcac acggcagaca aattgtcgga cagggcttcc
    34261 acgtcaaagc gggcgaaccc catgccgaag tccacgccct gcgtcaggcg ggggaaatgg
    34321 caaaaggcgc gaccgccttt gttaccctcg aaccgtgcag ccattacgga cgcacaccgc
    34381 cctgcgccga agccctgctc cgttccggcg taacacgcgt cgtcgccgcc atgcgcgacc
    34441 ccaacccgcc ggttgcaggc aaagggcttg tcctgctcaa agcagcgggc atcaagacgg
    34501 aatgcggact acttgaaaac aaagcacgcg aactcaaccg gggattttta tcgcgcatcg
    34561 aacgccgccg cccctttgtc cgcctcaaat gcgccgtttc gctggacggc aaaaccgccc
    34621 tttcagacgg cagcagcttt tggattaccg gcgaggaagc gcgtgccgac gtacaggttt
    34681 tgcgtgccga aagctgcgcg gtgctgaccg gcatcggcac ggtgttggcg gacaatcccc
    34741 ggctcaacgt ccgcgctttt ccaactttgc gccaacccgc gcgcatcgtt ttagacagcc
    34801 gcctgcgcct gcccccgaac agccatttgg ttaccgacgg acaatctccg accctcattg
    34861 ccacactcga gcgcaacgaa gacaggcttc gcccctatcg caaacacgcc cacgtccgca
    34921 tcctgatgcc gtctgaaacg gcaggcggca aaatcgacct gcaccgcctg atgcgcctcc
    34981 ttgccggcga aggatacggt gaagtgatgg tggaagccgg cgcaaaactc acatccgcct
    35041 ttttggcaga aaatttggcg gacgaaatcg ttttataccg ttcgcccaaa atcctcggca
    35101 tcggcagcgg tttattttgc ctgcccgaaa accgcgccgc cctttccgcg ccgccattat
    35161 ggacacccgt ttccaccgaa atcctcggac acgacatcaa aaccgtgttc cggaaaaacg
    35221 gcaacgcctt ttaaagggtt tgccgccgtt ttgctatata ataacgccga taaaaaacgg
    35281 cccgttcaga ccgccgcccg cccggaaaag cgcaacccgg actgccgcac cccgccggca
    35341 gccgcgacgg cctgaaagcc gtcaaattcc gatcaagaaa ggtttcagac agcacaggca
    35401 gcatcacgcc gccgcccgga catcaaaaat ggacacaaaa gaaatcctcg gctacgcggc
    35461 aggctcgatc ggcagcgcgg ttttagccgt catcatcctg ccgctgctgt cgtggtattt
    35521 ccccgccgac gacatcgggc gcatcgtgct gatgcagacg gcggcgggac tgacggtgtc
    35581 ggtattgtgc ctcgggctgg atcaggcata cgtccgcgaa tactatgccg ccgccgacaa
    35641 agacactttg ttcaaaaccc tgttcctgcc gccgctgctg ttttccgccg cgatagccgc
    35701 cctgctgctt tcccgcccgt ccctgccgtc tgaaatcctg ttttcgctcg acgatgccgc
    35761 cgccggcatc gggctggtgc tgtttgaact gagcttcctg cccatccgct ttctcttact
    35821 ggttttgcgt atggaagggc gcgcccttgc cttttcgtcc gcgcaactcg tgcccaaact
    35881 cgccattctg ctgctgttgc cgctgacggt cgggctgctg cactttccgg cgaacaccgc
    35941 cgtcctgacc accgtttacg cgctggcaaa ccttgccgcc gccgcctttt tgctgtttca
    36001 aaaccgatgc cgtctgaagg ccgtccggcg cgcgccgttt tcgcccgccg tcctgcaccg
    36061 ggggctgcgc tacggcatac cgctcgcact gagcagcctt gcctattggg ggctggcatc
    36121 cgccgaccgt ttgttcctga aaaaatatgc gggcctggaa cagctcggcg tttattcgat
    36181 gggtatttcg ttcggcgggg cggcattatt gctccaaagc atcttttcaa cggtctggac
    36241 accgtatatt ttccgtgcaa tcgaagaaaa cgccacgccc gcccgcctct cggcaacggc
    36301 agaatccgcc gccgccctgc ttgcctccgc cctctgcctg accggaattt tctcgcccct
    36361 cgcctccctc ctactgccgg aaaactacgc cgccgtccgg tttaccgtcg tatcgtgcat
    36421 gttgccgccg ctgttttaca cgctgaccga aatcagcggc atcggtttga acgtcgtccg
    36481 caaaacgcgt ccgatcgcgc ttgccacctt gggcgcgctg gcggcaaacc tgctgctgct
    36541 ggggcttgcc gtaccgtccg gcggcacgcg cggcgcggcg gttgcctgtg ccgcctcatt
    36601 ctggttgttt tttgttttca agacagaaag ctcctgccgc ctgtggcagc cgctcaaacg
    36661 cctgccgctt tatatgcaca cattgttctg cctggcctcc tcggcggcct acacctgctt
    36721 cggcacaccg gcaaactacc ccctgtttgc cggcgtatgg gcggcatatc tggcaggctg
    36781 catcctgcgc caccggaaaa atttgcacaa actgtttcat tatttgaaaa aacaaggttt
    36841 cccattatga aaatcatcct gactacttct atgtcggttc tgggaggcac gggcacgaca
    36901 tcatcgattg ccaaatgtcg tgcaaaaccg accccgttca gtccgacgaa atcgtccgcc
    36961 gcttcaggcg cgacatttcc tatcggaaaa tcgtcaacct gattgaaaga ttggcaaatg
    37021 agtaaagccg tcaaacgcct gttcgacatc atcgcatccg catcggggct aattgtcctg
    37081 tcgcccgtgt ttctggtttt gatatacctc atccgcaaga atctgggttc gcccgtcttc
    37141 ttcattcggg aacgccccgg aaaggacgga aaacctttta aaatggtcaa attccgttcc
    37201 atgcgcgacg cgcttgattc agacggcatt ccgctgcccg acagcgagcg gctcacgccc
    37261 ttcggcaaaa aactgcgcac cgccagtttg gacgaactgc ctgaactgtg gaacgtcctc
    37321 aaaggcgaga tgagcctggt cggcccccgc ccgcttttga tgcagtatct gccgctttac
    37381 aacaaatttc aaaaccgccg ccacgaaatg aaaccgggca ttaccggctg ggcgcaggtc
    37441 aacgggcgca acgcgctttc gtgggacgaa aagttctcct gcgatgtttg gtacaccgac
    37501 aatttcagct tttggctgga tatgaaaatc ctgtttctga cagtcaaaaa agtcttgatt
    37561 aaagaaggca tttcggcgca aggggaagcc accatgcccc ctttcgcggg gaatcgcaaa
    37621 ctcgccgtta tcggcgcggg cggacacggc aaagtcgttg ccgagcttgc cgccgcactc
    37681 ggcacatacg gcgaaatcgt ttttctggac gaccgcaccc aaggcagcgt caacggcttc
    37741 cccgtcatcg gcacgacgct gctgcttgaa aacagtttat cgcccgaaca attcgacatc
    37801 accgtcgccg tcggcaacaa ccgcatccgc cgccaaatca ccgaaaaagc tgccgcgctc
    37861 ggcttcaaac tgcccgttct gattcatccc gacgcgaccg tctcgccttc tgcaataatc
    37921 ggacaaggca gcgtcgtaat ggcgaaagcc gtcgtacagg ccggcagcgt attgaaagac
    37981 ggcgtgattg tgaacactgc cgccaccgtc gatcacgact gcctgcttga cgctttcgtc
    38041 cacatcagcc cgggcgcgca cctgtcaggc aacacgcgta tcggcgaaga aagccggata
    38101 ggcacgggcg cgtgcagccg ccagcagaca accgtcggca gcggggttac cgccggtgca
    38161 ggggcggtta tcgtatgcga catcccggac ggcatgaccg tcgcgggcaa cccggcaaag
    38221 ccccttacgg gcaaaaaccc caagaccggg acggcataag cgattaaaat aaacttccgt
    38281 acaaaccgat tttgacaaca cctgcggcgc gcgcccgatt cttcggaaca cgcccccttc
    38341 agacggcata gggtcggaaa tgccgtccga acccgacgga ccaaacacca tgctgaacac
    38401 cgccctttcc ccatggccga gcttcaccca agaagaagcc gatgccgttt ccaaagtcct
    38461 gctgtccaac aaagtcaact actggacggg cagcgaatgc cgcgaatttg aaaaagaatt
    38521 tgccgccttt gccggcacgc ggtacgccgt cgccctttcc aacggcacgc ttgcgctcga
    38581 tgccgcactc aaagcaatcg gcataggcgc gggcgacgat gtgattgtta cctcgcgcac
    38641 cttcctcgcc tccgcctcct gcattgtgaa cgcgggcgca aaccccgtgt ttgccgacgt
    38701 ggatttgaac agccaaaaca tcagcgcgga aaccgtcaaa gccgtgctga ccccgaatac
    38761 caaagccgtc attgtcgtcc accttgccgg tatgcctgcc gaaatggacg gcatcatggc
    38821 tttggcaaaa gaacatgatt tgtgggtgat tgaagactgc gcccaagcgc acggcgcgac
    38881 ctacaaaggc aaatccgtcg gctctatcgg gcacgtcggc gcgtggtcgt tctgtcaaga
    38941 caaaatcatc accaccggcg gcgagggcgg tatggttacg accaacgaca aaaccctgtg
    39001 ggaaaaaatg tgggcgtaca aagaccacgg caaaagctac gatgccgtgt accaccgcga
    39061 acacgcgccc ggtttccgct ggctgcacga aagtttcggc accaactggc gcatgatgga
    39121 aatgcaggct gtcatcggcc gcatccagct caaacacctg cccgaatgga cggcgcgccg
    39181 ccaagaaaac gccgccaagc tggcagaaag tttgcgcaaa ttcaaaagca tccgcctgat
    39241 tgaagtcgcc ggctacatcg gacacgcgca atacaagttc tacgccttcg tcaaacccga
    39301 acacctgaaa gacgactgga cgcgcgaccg catcgtcagc gaactgaacg cgcgcaacgt
    39361 cccctgctat caaggcggct gctccgaagt gtatttggaa aaagccttcg acaacacgcc
    39421 gtggcggccc aaagagcggc tgaaaaacgc cgtcgaactg ggcggcaccg ccctgacgtt
    39481 cttggtgcac ccgacgctga ccgacgacga aatcgcgttc tgcaaaaaac acatcgaagc
    39541 cgtcctgacc gaagccgcac gataaccctt cagacggcat atgccgcctg aaagcacata
    39601 ccgccccacg atatgaatct ggaaaccctg atcgccctgc cgcgcaacat caagaaaatc
    39661 tgtttcctca tacacgattt tctgatgatt ttcaccgcct tttggttcac ccaaagcctg
    39721 aaggccgact actcggacga atggttcgat tttgccaact ggcagtcttt tttgctgact
    39781 gccttgctga ccatcacatt atttatccga atggggcttt accgcgccgt tacacgcttt
    39841 gtcagtttcc acgtcctgac caccgccttt gccggcagcc tcgcctccgc cgtattgttc
    39901 ttcctcaaca cattgatttt tgaagaaagg ctgcgcctcg ccctgccgat tgtctatttc
    39961 ttactgctgt ttgtttccgt gaccggctcg cgtatggttt tgcgcggact cctgtccgac
    40021 caccagaaaa aaacacatga ccccgtcatc atttacggcg cggggcgttc gggtcggcag
    40081 ctccttgaag ccgtcaaaca gatgcgcgaa tattccgccg ccgcctttgt ggacgacaac
    40141 cccaagcttt ggcacaccgt catctacgac cttgccgttt accagcccga tgccatcgcc
    40201 ttcctcatcg aacgctacgg cgtggaaaaa atcctgctcg ccattcccag cgcgactcag
    40261 gaacaacgcc gccgaatcat cagcaaactg aaagcctatc cgtgcgaagt gttgaccatt
    40321 cccggaatga aagacctgat ggacgggaaa atcagcatcg gcacgctcaa aaaaatctct
    40381 gtgtccgacc tgctcgggcg cgattccgtc gcacccgacg accgcctcat gaatgccgac
    40441 accgaaggca aaaccgtcat ggtaaccggc gcgggcggct ccatcggttc ggaactctgc
    40501 cgccagatta tccgccgccg ccccgaaagg ctgctgctgt tcgagctgtc cgaattcgcc
    40561 ctgtacaccg ttgaaaaaga attgtgcgaa tactgcgccc gaaacggcat cgccgccgaa
    40621 atccttccct ttttgggttc ggtgcaaaac cgcacgctgc tcacgcacat tatgaccgcc
    40681 ttttccgtta cgaccgtcta tcacgccgcc gcctacaaac acgtccctat ggtcgagttc
    40741 aacaccgtcg agggcatacg caacaacatc ttcggcacac tcgaatgcgc gcttgccgcc
    40801 acggcttcgg gcgtggaaac cttcgtcctc atctccaccg acaaagccgt ccgccccgcc
    40861 aacacgatgg gtgcgggcaa acgcatggcg gaactctgcc ttcaggcact cgccgccgaa
    40921 cccggacaaa aaacccgctt cagcatggta cgtttcggca atgttttagg ctcgtccggc
    40981 tccgtcgtcc cgctgtttga aaaacagatt gccgaaggcg gccccatcac cctgacccac
    41041 cccgacatca cgcgctattt catgaccata cccgaagcgg cgcaactggt tatccaagcc
    41101 ggcgcgatgg gcagaggcgg cgacgtgttc gtcctcgata tgggcgaatc cgtcaaaatc
    41161 accgaccttg cccgccaaat gattacctta agcggactca aacccaaaac gcccgaacag
    41221 cccgacggcg acatcgaaat cctcattacc gggctgcgcc ccggcgaaaa actctacgaa
    41281 gagttgctca tcggcgacaa cgtccgcaaa accggccatc cgcgcatcat gaccgccgac
    41341 gagaccatgc tgccgtggca cgaactctcc gccctgctcg accgcatccg caccgcctgc
    41401 gaccgttacg accagcaggc aatccgcgac ctgctcgtca acgccccgac cggctttacc
    41461 ccgaccggcg gcatctgcga cctgctttgg gtatgcgaaa cacacagaaa aaatgccgtc
    41521 tgaaccttca gacggcatac cgtacaaacc aatctgcctt acacacactg agtttgacat
    41581 gcagttctcg gcattcggcg aaaaattcac gcaacacagc ggcatcctgc aactgatgga
    41641 cgacctcggc gacgcgctca aaagcgacaa gcccgtcaat atgctcggcg gcggcaaccc
    41701 ggcgcgcatt cccgaaatcg atcgggcgtt cgccgacata ttttccaagc tggcggcgga
    41761 acacgccgtc gagaacatcg gcaactactc caatccccaa ggcgatgccg cgctgattga
    41821 cgcgctgacc gccttcccca accgcgaata cggctggaac ctgaccgtcg gcaatatcgc
    41881 gctgaccaac ggttcgcaaa acgcgttttt ctatttattc aacctcttcg gcggcaaatt
    41941 caagctttca gacggcacat ccgcagaaaa agccattttg ttgccgctcg cgcccgaata
    42001 catcggctat gccgacgtgc atgtcgaagg gcggcacttc gtttccgtca aacccaaaat
    42061 cgaaaacgtc gaacacgaag gcgaagcagg cttcttcaaa taccgcgtgg actttgacgc
    42121 actggaaaac ctgcccgaac tcaaagcggg caaaatcggc gcgatttact gttcgcgccc
    42181 gaccaacccg accggcaacg tgttgaccga cggcgaaatg gcgcgtttgg acgctttggc
    42241 gcgtgaacac ggcattccgc cgattatcga caacgcctac ggaatgccgt tccccaacat
    42301 tattgacagc ggcgtaacgc tgaattggca cgaaaacatc atcctctgct tcagcctttc
    42361 caaagtcggc ctgccgggcg ttcgcaccgg catcatcgtc gccgcgcccg aagccgtcaa
    42421 agccgtcagc agcctgaacg cgattgtgaa ccttgccccc acgcgcttcg gcgcggccat
    42481 cgccgccccg ctgctggaaa gcggcgagat gaaacggctt gccgaccaag tcatccggcc
    42541 gttttaccgc aatcaggcgc aaaccgccgt ttcgctgctc aagcgcgagc tgggcgcgta
    42601 cccgatgaaa atccacaaac ccgaaggcgc gattttcctg tggctctggt ttgaaaacct
    42661 gcccgtttcg tcgcaaaccc tgtacgaaat gctcaaagcc gaaggtacgc tgattattcc
    42721 gggcgaacat ttcttcgtcg gcatcgacac gcaagactac ccgcacgcgg gcgagtgcat
    42781 ccgtatgagt atcgcacagg acgctcaaac gctggaaaaa ggcatcgccg ccatcggcaa
    42841 aaccgtccga aaaccgtacg acaacgttta aaacgcaaaa aatgccgtct gaagaccttt
    42901 cagacggcat tttcatccgc attcaatgtc gggaaaaatg cgcccgcacc ggtttgcagt
    42961 tttccggaag atcgggacac gcgccgagga tgccgccgct gaagtcgtct aaacggtgga
    43021 agtcgctgcc cgcgcttgcc aacaatccga agcggtctgc caaaagcgcg tagttgaggc
    43081 ggtcgttttt gcagcagttg ccgctgtgga cttcgatgcc cgcgccgccg aggtttttaa
    43141 attcttcaaa caggttgcgc ttggcggttg ccgacaaatc ataacgcatc ggatgggcaa
    43201 tcaccgccat gccgcccgcg ccgttgacgg cggagacgca gtctgccagc gtcgcccatt
    43261 cgtggcgtac cgcgcaggat ttgccgtcgc ccaagtattt ggtgaacgcc tgctgcttgt
    43321 ttttgacgtg tccgtttttg atgaggaact cggcgacgtg ggtgcggctg accatttctt
    43381 tgtttgccgc cagcgccagc gcgccgtcgt atgcgccgcc gatgcctttc ttttcgagct
    43441 tggcggcgat ggcttcaaga cgtttcagac ggcctttgcg gacatctgcc aacaggtttt
    43501 gcaggttttc gtcctgctcg tcgaaatcca aaccgacaac gtgtatggta cgcccgcgcc
    43561 acgttaccga aatttccacg ccgttaatca ggcgcaaacc gagcctgtcg gcttcggcac
    43621 gcgcttcgga gatgccgccc gtgtggtcgt ggtcggtcaa cgccagcagc gtgcagccgt
    43681 tttgatgtgc gaggcggacg acttcggcgg gggggagcat accgtcggaa acggtggaat
    43741 ggcagtgcag gtctatcatg gggtgttatg tggtggtgaa tgaaggacgg gatgtttcaa
    43801 tgttgtcgga cagtgtggcg gcggcggtca ttttaaaaaa caggtgtatg gcaaatgcaa
    43861 aggaaaagcc gctatgccgt cattcccgcg caggcgagaa tccagacttt gatttattgg
    43921 aaatgtttaa ggttaaccgc tatttcaaac ttccggattc ctgctttcga gggaatggcg
    43981 atatgaacgt tttcaatttt aatctactat aaaagaccgt ctgaaaacgt ggttttatca
    44041 agcccaaccg ttttcagacg gcatcgtccg gccaacccgc ttccttcagt tttttccgtt
    44101 gcgcatcgta gcgtgccttg tccgcccagt aaatcgtctg aatgcacgcc tcgccgcagt
    44161 cgctgccgca gcattcccac gactcggggc ggacgggttc gtccaaaagc ggctcgccca
    44221 aaagggcttc ggctttatgc ttcagggtcg tatccatcgg cgatttccaa gcgcgcgccg
    44281 tcaaactcga tgacttctcc gccgcgtatt ttggccgttt tgcggatttc ggttccgccg
    44341 ttgcgcaaca ccagcccttc ggcgataaat gcttttgcct gtccgccgct ttcggcaagt
    44401 ccggccaatt tcaagaggtc gcacaaggcg atgtattcgt tgtcttcgag atagacggtg
    44461 gcttccataa tgtccccttt tagaaagagg gcgttattgt agcacctgcc gccgccgcac
    44521 ccaaaattgc cgcaaaaccg gcgatgtgtc cgcactgcct gttccgtaaa aatgccgtct
    44581 gaaaccccgt atgccgccat ccgtccaaag aaatcctgcc caacggcagg ctgcaaatcc
    44641 tgttccccga cgaatccgca ttgacgctga tgcacatcct caaacgcgga ctgcccgata
    44701 caccggcaat cggcatcaaa acgaaatcaa aaacctgttt gaacgtaaaa tctatttaac
    44761 tgcttgccgt ccgtccgaat aaggtatata gttccttata actagtttga tagtccttta
    44821 tatctatcaa tactccttgg gaagcctccg ccacacggca ggaggcattt ttttgccgtc
    44881 gtaaaagctc aaaaaacatt tgcaggtcat gccgtctgaa tccgaaacgg cattacctgc
    44941 accgccatct aaagacaacc ctgctacaat acgcctttta ttgtccacgc cgattttgcc
    45001 atgaccgagc cgacctacat ccccctgcgc ctgcataccg aattctccat taccgacggt
    45061 atggtgcgga ttaaaaaact gattgccaaa gcgcaagaat acggtttgcc tgctttgggc
    45121 atcagcgatt tgatgaacga attcggtttg gtgaaattct ataaagcctg ccgcggcgca
    45181 gggattaagc ccgtcggcgc ggcggacgtg tggataggca atccgaatgc gcccgacaag
    45241 ccgttccgcg ccatgctggt tatccgcaac gatgcgggtt atctgcgctt gagcgagctt
    45301 ttgacggagg cttatgtcgg ccaagaccgc aatatccatc atgcggaact caatcccgaa
    45361 tggctggaaa acggcgacaa cagcggcttg atttgtttga gcggcgcgca ttacggcgaa
    45421 gtgggcgtga atctgttgaa cggcaatgaa gacgcggcgc gtgcggcggc gttgaaatat
    45481 gcggcatggt ttcccgatac gttttattta gagctgcaac gcctgcccga acgccccgaa
    45541 tgggaggctt gcgtttccgg cagcgtgaag ctggcggagg aactgggttt gccggtggta
    45601 gcaacgcatc cgacacagtt tatgaaccgc gacgatttca acgcgcacga ggcgcgggtg
    45661 tgcatcgcgg gcggctgggt gctgacggac aagaaacgtc cgcgcgattt cacgccgagc
    45721 cagtttttca ttccgccgga aacgatgctg gagcgtttcg ccgacttgcc cgaagccttg
    45781 gaaaacacgg tggaaatcgc caaacgctgc aacttgcaca tcacattggg caaaaacttc
    45841 ctaccccttt tccccacgcc cgacggttta tcactcgatg actgtctcat caaactttcc
    45901 aacgagggtt tgcaggaacg catggttcag ctttatcccg acgaggcgga acgggcggca
    45961 aaaatgccgg aatatcagga gcggctggac tttgagctga acatcatcat ccaaatgaaa
    46021 ttccccggct atttccttat cgtacaagac tttatcaact gggcgaaaac gcacggctgt
    46081 ccggtcgggc cgggacgcgg ttcgggcgcg ggttcgctgg tggcgtattc gctgaagatt
    46141 accgacctcg acccgctgaa atacgcgctg ctgttcgagc gtttcctaaa ccccgaacgc
    46201 gtttccatgc ccgactttga cgtggacttc tgtcaggcaa accgcggccg cgtgattgaa
    46261 tacgtgcgcg aaaaatacgg cgctcaggcg gtcagccaga ttgtgacttt cggcacgatg
    46321 tcgtccaaag cggtcatccg cgacgtgggg cgtgtgctgg aactgccgtt tatgctgtgc
    46381 gacaaactgt ccaagctgat tccgttggaa gccaacaaac ccctgagttt ggacgacgcg
    46441 atgaaggcgc agccgcagat tcaggaattg cttgaagcgg aagaagcgga cgaactgatt
    46501 acgctggcga aaaaactgga agacctgacg cgcggtttgg gtatgcacgc aggcggcgtg
    46561 ttgattgcgc cgggcaagat ttccgattac agccccgtgt atcaggcgga cgaatccgcc
    46621 tcgcccgtat ccatgtacga caagggcgac gtggaagacg tgggtttggt gaagttcgac
    46681 tttttgggcc tgcgcaacct gaccattatc gaaatggcgc agaacaacat caaaaacacc
    46741 gccggcgata tcgtcgatgt cggcaagatt ccgcttgacg accagactgc ctaccaaatc
    46801 ttccgcgatg cgaacaccac cgccgtcttc cagttcgagt cgaccggcat gaaaaaaatg
    46861 ctgaaaacgg cgcatacgac caagtttgaa gaactcatcg ccttcgtatc gctctaccga
    46921 cccggcccga tggacaacat tcccgacttc gtcgcacgca tgaagggaca ggaattccaa
    46981 tacatccacc cgctgctgga aggcatcctc gcgccgacct acggcattat ggtgtatcag
    47041 gaacaagtga tgcaggcggc gcagattatc ggcggctact cgctcggcgg cgcggacctg
    47101 ctgcgccgcg ccatgggtaa gaaaaagccc gaagagatgg tgaaacaccg cgaaatcttc
    47161 gccgaaggtg cggcaaaaca aggcatttcg cgcgaaaaat ccgacgaaat cttcaactac
    47221 atggaaaaat tcgccggcta cggtttcaac aaatcccacg ccgccgccta cgccctgatt
    47281 tcctaccaga ccgcatggct caaagcccac tatcccgccg aatttatggc ggcgacgatg
    47341 tcgtccgaat tggacaacac cgaccagctc aaacatttct acgacgactg ccgcgccaac
    47401 ggcatcgagt tcctgccgcc cgacattaac gaatccgact accgcttcac gccgtatccg
    47461 aacatgaaaa tccgctacgc actcggcgcg attaaaggca cgggcgaagc cgccgtcgaa
    47521 tccatcatcg ccgcgcggca aagcggcggc aaatttaccg gcctgttgga cttctgcgag
    47581 cgcgtcggca aagaacacat gaaccgccgc accctcgaag ccctgatacg cggcggcgcg
    47641 ttcgacagca tcgaacccaa ccgcgccatg ctcttggcga acatcgacct tgccatggac
    47701 aacgccgacc aaaaagccgc caacgccaat cagggcgggc tgtttgacat gatggaagac
    47761 gccatcgaac cggtgcggct catcgacgca cccatgtgga gcgaatcgga aaaactcgcc
    47821 gaagaaaaaa ccgtcatcgg cttttacctg tccggccacc cgttcggccc gtatgcccaa
    47881 gaagtccgcc aaatcgcccc gaccaaatta ggccgtctga aaccgcaaga cagcgtgcgc
    47941 ctcgccggat tcgttaccgc cgtgcgcacc atgatgggca aacgcggcaa aatcgccttc
    48001 gtcagcctcg aagattcgag cgggcaggct gaaatcatgg tcggcggaca aacgctggaa
    48061 aactgcgccg actgcctcaa agccgaccaa gtgctgatta tcgaatccaa agtcagccgc
    48121 gacgattacg gcggcggcga cgggctgcgc atcatggcaa accaagtcat gaccctgcaa
    48181 acggcgcgcg aacgctacgc ccgcagcctc agcctcgccc ttgccccgca tcacgacatc
    48241 ggcggactgg tacggctgct cgccgcccac caactgcccg acacgccgcg catcccgctt
    48301 caattgtcgt atgccaacga aaaagcgtcg ggcaggcttc aagtgcctcc gaaatggacg
    48361 gttacaccga gttccgcatt gttcggcgaa ctggaaacat tgctcggcag ccggtcggtg
    48421 cgcgtgaatt ggtaaccaaa aatacaaatg ccgtctgaag cccaaaaccg gtttcattcg
    48481 tactttattc gaatgattga ataaaagtaa ctgccaagaa aaacgtattt tttggttatt
    48541 tcgccagcct aaataaatca accggggcga ttgatatccg tgtacatgac acagacagcg
    48601 ccaaagggaa aaacggcatt ttcaaaagta tcggtgtcaa aaccgccctt tcattccaaa
    48661 aataccaaat cgacgaaccg ggcaaagaaa tcagaccgtg ccgcctgaaa aaacgcccgc
    48721 ccgtccgtta aattaatcta tccctgtttc aggcggcctg aaacagggat ttttatatca
    48781 agataaaatg agaacgggag cgacaaccac gggcaaagtc ggcgcgaact atcaaaaatt
    48841 gtagcactgc gaaatgagaa cgggagcaat aacccttagg tagctcttgt tatagcggat
    48901 taacgaaaat caggacaagg cgacgaagcc gcagacagta caaatagtac ggaaccgatt
    48961 cacttggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcgg ggcaacgccg
    49021 taccggtttt tgttaatccg ctatattttc cgatgcaaaa caaagcagtc atatatttga
    49081 ttccccctac cgttgccacg cttccctcaa atattcgacg cagtcggtca gcgagtaaaa
    49141 cgggacattg ccgtggtcgg cattcggata ttcccggaaa aatacggcgg tcccgcgcct
    49201 gtccaactct gccgccattt gttcggcctg ccctgccatg tcgcgttctt ccctgcgttt
    49261 gcaaccgctg ctccgctcga gtgcgccgat gttgaggcag gcatcgatgc cgtccagccg
    49321 gttttcagac ggcataaagt cgagtatccg cctgttgtgc caccaaatcg aggggatacg
    49381 agccaatgcc gtctgaaaca gcggtgggaa agcagggaat acagtccgaa cagtgcgccg
    49441 aacgagtgtc cgaatacggc ggtttcatta cggttaaggg tgtagcggct ttctaaaaag
    49501 gcggtcagtt cgctgtcgat aaagtcggcg aagcggtctg cctgtccgaa ctgccgccgt
    49561 tcgtctgctg tggcgttgtc tccaagcggc ggcgtgtagt cggcggcacg ctgcgccagg
    49621 tcgcgcacac tgcctgtcgt gtagccgata ccgacaatca ggcagggggc gttgcttcgg
    49681 gtaacggggt tgttcatcag cgactgcatg atgttgtaca gcgcgggaaa aaggcttcgc
    49741 cgtcgaggac aaagaggacg ggatagcctt cagactgcat ttcgccgagt gttgccgtct
    49801 gaatccgata gattcgcccc gtgcaggtgg atttgatttc ggtttcaaag gcttggggca
    49861 gtatggcagg ttggaatgtg tcggtcggta tgggtttcat gatgttcggc ttgtgggtca
    49921 gactggcggc aagcggtaaa accgaaccgg caaagcggtc tgcctgccgg ttcggtctgt
    49981 tttccttcgc aatgccatac tccggttgtg agcgcatagg gtatgccgcg cagcttgttg
    50041 tggtttgatt atgctgcggc tgccgtttca gacggcatat tggtctttaa aaactgtaac
    50101 gcaggtttgc cgtcaggctg cgctccgaac cgggaatgtt aaaggtgttc gtgccgccga
    50161 cgcgggcgta gtaatggcgg ttgaagatgt tgtcggcatt gatttgcagc ttcagcttgg
    50221 gcgtgaagcg gtatgccgcc atcgcatcga acgtggcata accgcctgca tgcatccctg
    50281 cagatgaagt aatgccgctc atcgcgttca cgccgccgcc gacggtcagc ccgggcgtaa
    50341 cttggtaagt cgtccacagg tttgcgctgt gtttgggcac cagcaggaag atgccgtcgt
    50401 cgcgtggatt ggcggcggtt ttgatttggc tgtgcagata gctgtaacct gcatggattt
    50461 gccatttcgg tgtaatcgca ccgctgattt cggtctcaac gccttccatc acgcgtttgc
    50521 ccaatgcggc gtaacgggtg ttgcggttgt tcgggttcgg cggcgttttt gtctttcagg
    50581 cggcaggggg agacgcgggt attgagtcgg tcatccatat agctgccttt aacgagctgt
    50641 aatcgccgtt tttctcggtg gcaatcgcgt tgtacgaacg ctgccctttg atatggacgg
    50701 tttccaaatc cgccgtttgt gcggcaaaaa ccggagacga gagtgctgcc aaaaccgtgg
    50761 cggcggtcat attgatgcgg aaaactgaca taaactgtcc catctgtata aatgataatg
    50821 gttctatttt aataaagcac aacgcggctt gttcggaaaa acatatcgct cagccgacaa
    50881 attttgtcga aaatgcgaca cgtctgcgtt ttccgcataa aatctgcttt tttactgcaa
    50941 ccaacctgcc atgaccccgc ccaaactcat catcttcgac tgggacggca cgcttgccga
    51001 tacgacccaa cccatcatcg acaccatgcg ccgcagcttc gccgaatgcg gttttccgcc
    51061 gcccgaagcg gagcgcgtcc gcagcctgat cggatacagc ctgcccgaaa tcatccgcgc
    51121 cctgctcgaa atgccttctg aagctgccgt tgccgacatc gcgcgcactt attccgcgca
    51181 ttacctcaat cccaacaacc gcaatatgac actctttccc gatgccccgc cctgtctgga
    51241 caagctcaaa gcgcaaggct tccggcttgc cgtcgccacg ggcaaagggc gggcgggttt
    51301 ggacaacgcc atcagccaaa ccgccaccgg cggctattgg ctcgccaccg cctgcgcggg
    51361 ggaatacccc tccaaaccct cgcccgaaat ggtattcgga ctctgcggcg aactgggact
    51421 cgacccgaaa gaggcattgg ttgtcggcga tacggcgcac gacctgcata tggcggcaaa
    51481 cgcaggcgcg gcggcagtcg gcgttgccac cggcgcacat tcgcgcgaac agctccttgg
    51541 cgcaccgcat ctcgccgtat tggacggttt gtccgaactg cccggttttc ttgcacgaca
    51601 ttacgcctga ttggtttccg catccggcac gcggcaaaaa tgccgtctga agcctgttca
    51661 gacggc
//
LOCUS       DAXFWM010000005        51659 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000005 DAXFWM010000000
VERSION     DAXFWM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 51659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51659
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.005"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..230)
                     /locus_tag="ACFI8B_000256"
     CDS             complement(<1..230)
                     /locus_tag="ACFI8B_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3833567.1"
                     /translation="MKPKFTFIDLFAGIGGFRIAMQNLGGEYVFSSEWDEKAKLTYEA
                     NFGEVPFGDITLEEIKQYIPKQFDVLCGFTLS"
     gene            complement(337..750)
                     /locus_tag="ACFI8B_000257"
     CDS             complement(337..750)
                     /locus_tag="ACFI8B_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC3833568.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(767..1756)
                     /gene="pheS"
                     /locus_tag="ACFI8B_000258"
     CDS             complement(767..1756)
                     /gene="pheS"
                     /locus_tag="ACFI8B_000258"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC3833569.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(1997..2356)
                     /gene="rplT"
                     /locus_tag="ACFI8B_000259"
     CDS             complement(1997..2356)
                     /gene="rplT"
                     /locus_tag="ACFI8B_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC3833570.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(2369..2566)
                     /gene="rpmI"
                     /locus_tag="ACFI8B_000260"
     CDS             complement(2369..2566)
                     /gene="rpmI"
                     /locus_tag="ACFI8B_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC3833571.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(2713..3234)
                     /gene="infC"
                     /locus_tag="ACFI8B_000261"
     CDS             complement(2713..3234)
                     /gene="infC"
                     /locus_tag="ACFI8B_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC3833572.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(3252..5165)
                     /gene="thrS"
                     /locus_tag="ACFI8B_000262"
     CDS             complement(3252..5165)
                     /gene="thrS"
                     /locus_tag="ACFI8B_000262"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC3833573.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(5218..5294)
                     /locus_tag="ACFI8B_000263"
     tRNA            complement(5218..5294)
                     /locus_tag="ACFI8B_000263"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5258..5260),aa:Val,seq:gac)
     gene            5473..6588
                     /gene="tgt"
                     /locus_tag="ACFI8B_000264"
     CDS             5473..6588
                     /gene="tgt"
                     /locus_tag="ACFI8B_000264"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC3833574.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGVD"
     gene            6598..7680
                     /gene="hemH"
                     /locus_tag="ACFI8B_000265"
     CDS             6598..7680
                     /gene="hemH"
                     /locus_tag="ACFI8B_000265"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC3833575.1"
                     /translation="MPSEAASDGILPPYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            7871..8338
                     /locus_tag="ACFI8B_000266"
     CDS             7871..8338
                     /locus_tag="ACFI8B_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFC3833576.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTAFPPLFRSDYIMNKPHVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQIKPF"
     gene            complement(8335..10056)
                     /locus_tag="ACFI8B_000267"
     CDS             complement(8335..10056)
                     /locus_tag="ACFI8B_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC3833577.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGGGNIGS
                     MGLKVPR"
     gene            10260..11000
                     /locus_tag="ACFI8B_000268"
                     /pseudo
     CDS             10260..11000
                     /locus_tag="ACFI8B_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            11069..12607
                     /gene="lnt"
                     /locus_tag="ACFI8B_000269"
     CDS             11069..12607
                     /gene="lnt"
                     /locus_tag="ACFI8B_000269"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC3833578.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            12871..13734
                     /gene="rpoH"
                     /locus_tag="ACFI8B_000270"
     CDS             12871..13734
                     /gene="rpoH"
                     /locus_tag="ACFI8B_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC3833579.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(13891..14367)
                     /locus_tag="ACFI8B_000271"
     CDS             complement(13891..14367)
                     /locus_tag="ACFI8B_000271"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFC3833580.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKHLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSICSKHLSIPPICRKSS"
     gene            14467..15351
                     /locus_tag="ACFI8B_000272"
     CDS             14467..15351
                     /locus_tag="ACFI8B_000272"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC3833581.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(15442..15957)
                     /locus_tag="ACFI8B_000273"
     CDS             complement(15442..15957)
                     /locus_tag="ACFI8B_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC3833582.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(16033..16452)
                     /locus_tag="ACFI8B_000274"
     CDS             complement(16033..16452)
                     /locus_tag="ACFI8B_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833583.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(16573..17571)
                     /gene="holA"
                     /locus_tag="ACFI8B_000275"
     CDS             complement(16573..17571)
                     /gene="holA"
                     /locus_tag="ACFI8B_000275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC3833584.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGNAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(17573..18052)
                     /gene="lptE"
                     /locus_tag="ACFI8B_000276"
     CDS             complement(17573..18052)
                     /gene="lptE"
                     /locus_tag="ACFI8B_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC3833585.1"
                     /translation="MNKIFLTAAALVLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(18104..18883)
                     /gene="pgeF"
                     /locus_tag="ACFI8B_000277"
     CDS             complement(18104..18883)
                     /gene="pgeF"
                     /locus_tag="ACFI8B_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC3833586.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILRREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            19035..19259
                     /locus_tag="ACFI8B_000278"
     CDS             19035..19259
                     /locus_tag="ACFI8B_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833587.1"
                     /translation="MMPLYTFSELYMLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGF
                     NRKGMGIKSISDTSRAIPSENQSPLSDGIV"
     gene            19336..20283
                     /locus_tag="ACFI8B_000279"
                     /pseudo
     CDS             19336..20283
                     /locus_tag="ACFI8B_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(20436..21560)
                     /gene="rluD"
                     /locus_tag="ACFI8B_000280"
     CDS             complement(20436..21560)
                     /gene="rluD"
                     /locus_tag="ACFI8B_000280"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC3833588.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            21559..22362
                     /locus_tag="ACFI8B_000281"
     CDS             21559..22362
                     /locus_tag="ACFI8B_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC3833589.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(22432..24651)
                     /locus_tag="ACFI8B_000282"
     CDS             complement(22432..24651)
                     /locus_tag="ACFI8B_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC3833590.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            24683..24958
                     /locus_tag="ACFI8B_000283"
     CDS             24683..24958
                     /locus_tag="ACFI8B_000283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833591.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            25037..29635
                     /locus_tag="ACFI8B_000284"
     CDS             25037..29635
                     /locus_tag="ACFI8B_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFC3833592.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLNQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTGHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGNVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEALR
                     QQAKAEQVKRQQAAEAEKVARQKDEEAKRKAAEIARQQEEARKAAELAAKQKAEAERK
                     ARELARQKAEEASHQANAKPKRRRRRAILPRPPAPVFSLDDYDAKDNSESSIGNLARV
                     IPRMGRELINDYEEIPLEELEDEAEEERRQATQFQPKSRNRRAISSEPSSDEDASESV
                     STSDKHPQDNTELHEKVETAGLQPRAAQPRTQAAAQADAVSTNTNSALSDAMASTQSI
                     LLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRS
                     LSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSR
                     LQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVKVSS
                     MAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAYKADNQQQYS
                     AGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(29711..30913)
                     /gene="trpB"
                     /locus_tag="ACFI8B_000285"
     CDS             complement(29711..30913)
                     /gene="trpB"
                     /locus_tag="ACFI8B_000285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC3833593.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEATGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(30996..31625)
                     /locus_tag="ACFI8B_000286"
     CDS             complement(30996..31625)
                     /locus_tag="ACFI8B_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC3833594.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            31901..32680
                     /gene="rsmA"
                     /locus_tag="ACFI8B_000287"
     CDS             31901..32680
                     /gene="rsmA"
                     /locus_tag="ACFI8B_000287"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC3833595.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            32695..33264
                     /locus_tag="ACFI8B_000288"
     CDS             32695..33264
                     /locus_tag="ACFI8B_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC3833596.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            33375..33830
                     /locus_tag="ACFI8B_000289"
     CDS             33375..33830
                     /locus_tag="ACFI8B_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833597.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            33872..34600
                     /locus_tag="ACFI8B_000290"
     CDS             33872..34600
                     /locus_tag="ACFI8B_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3833598.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            34673..35032
                     /locus_tag="ACFI8B_000291"
     CDS             34673..35032
                     /locus_tag="ACFI8B_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833599.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            35037..35477
                     /locus_tag="ACFI8B_000292"
     CDS             35037..35477
                     /locus_tag="ACFI8B_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC3833600.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            35508..36782
                     /gene="folC"
                     /locus_tag="ACFI8B_000293"
     CDS             35508..36782
                     /gene="folC"
                     /locus_tag="ACFI8B_000293"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC3833601.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            36796..37836
                     /gene="ftsN"
                     /locus_tag="ACFI8B_000294"
     CDS             36796..37836
                     /gene="ftsN"
                     /locus_tag="ACFI8B_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC3833602.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRSAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            37829..38326
                     /locus_tag="ACFI8B_000295"
     CDS             37829..38326
                     /locus_tag="ACFI8B_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC3833603.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            38520..40064
                     /gene="purF"
                     /locus_tag="ACFI8B_000296"
     CDS             38520..40064
                     /gene="purF"
                     /locus_tag="ACFI8B_000296"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC3833604.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYRTGDIDDAYLDRLSAEKSGCAGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            40164..40655
                     /gene="greB"
                     /locus_tag="ACFI8B_000297"
     CDS             40164..40655
                     /gene="greB"
                     /locus_tag="ACFI8B_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC3833605.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            40714..41340
                     /locus_tag="ACFI8B_000298"
     CDS             40714..41340
                     /locus_tag="ACFI8B_000298"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC3833606.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(41815..44646)
                     /gene="lbpA"
                     /locus_tag="ACFI8B_000299"
                     /gene_synonym="tbpA"
     CDS             complement(41815..44646)
                     /gene="lbpA"
                     /locus_tag="ACFI8B_000299"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC3833607.1"
                     /translation="MNRRHTFPLTLTALAVAAAFPSYAANPETAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRQNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(44643..46784)
                     /locus_tag="ACFI8B_000300"
                     /pseudo
     CDS             complement(44643..46784)
                     /locus_tag="ACFI8B_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(46937..47860)
                     /gene="era"
                     /locus_tag="ACFI8B_000301"
     CDS             complement(46937..47860)
                     /gene="era"
                     /locus_tag="ACFI8B_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC3833608.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(47907..48626)
                     /gene="rnc"
                     /locus_tag="ACFI8B_000302"
     CDS             complement(47907..48626)
                     /gene="rnc"
                     /locus_tag="ACFI8B_000302"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC3833609.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSSGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            48793..49116
                     /locus_tag="ACFI8B_000303"
     CDS             48793..49116
                     /locus_tag="ACFI8B_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC3833610.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            49163..49639
                     /gene="ribH"
                     /locus_tag="ACFI8B_000304"
     CDS             49163..49639
                     /gene="ribH"
                     /locus_tag="ACFI8B_000304"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC3833611.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            49717..50142
                     /gene="nusB"
                     /locus_tag="ACFI8B_000305"
     CDS             49717..50142
                     /gene="nusB"
                     /locus_tag="ACFI8B_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC3833612.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(50221..51255)
                     /gene="pyrC"
                     /locus_tag="ACFI8B_000306"
     CDS             complement(50221..51255)
                     /gene="pyrC"
                     /locus_tag="ACFI8B_000306"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC3833613.1"
                     /translation="MQTLTIIRPDDMHLHLRDDDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(51316..51513)
                     /locus_tag="ACFI8B_000307"
                     /pseudo
     CDS             complement(51316..51513)
                     /locus_tag="ACFI8B_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12938 a        14633 c        12534 g        11554 t
ORIGIN      
        1 tttgataagg taaacccaca cagcacatca aattgcttcg gaatgtattg tttgatttct
       61 tccaaagtaa tatcgccaaa cggcacttct ccgaaattgg cttcgtacgt cagtttggct
      121 ttttcgtccc actcgctcga aaacacgtat tccccgccca agttctgcat cgcgatgcgg
      181 aagcccccga tacctgcgaa caagtcgata aaggtaaatt tgggtttcaa ggccgtctga
      241 aaagtatcgt tgttttcaaa aaggcggtat tgtgctgctt tttcttttaa ccgttcatcc
      301 tgcccatctt ccttaatctt gtttttgtaa atcaattcat cttgtttgga tgatttcttt
      361 gactttttcg gcacagcagg ttgtattttt gacgacatcg ttactccaaa aacgtaaaac
      421 cgtccagccc tcggctttca gacggcctgt tacttctata tccctttgga tattgcgctc
      481 aattttggca atccaaaact cacgatttcc ctttattacc gcttttttct gttcccaatc
      541 cttaccgtgc cagaattccc catcgacaaa tacggcaact ttatattttt taaatgaaaa
      601 atcaggtttt ccaaaaatgc ttccgctatt tttccgatac ctgagcccca aagcccacat
      661 tgccttcgcc aacacaagct cgggtttcgt ccctgtactt ttgttggact gcatacattt
      721 tttgcgctgc tccggggtta atttatccat tgggtttatt tgagatttat tcaaactgct
      781 tcaagaagtt caaatcgtta tcgaagaaca agcgcaggtc attgacgttg tagcgcaaca
      841 tggcaaagcg gtcgagacca ataccgaaag cgaaaccggt atatttttca gggtcgatat
      901 tgacattttt caacacatta ggatgtacca taccgcaacc gcctacttcc aaccatttgc
      961 cgttttctcc catgatgtcg atttcggcag aaggctcggt aaatgggaag aaagacggac
     1021 ggaaacgcac ttgtaggtca tcgcgttcaa agaagcgacg gataaagtca gtgaacactg
     1081 cttttaagtc ggcaaaagtt acgccctctt ctacccacaa accctcggcc tgatggaaca
     1141 taggcgagtg cgtggcatcg ctgtccacac ggtaaacgcg gccgggggcg ataatgcgga
     1201 tgggcggctc ttttttatcg agcatatagc ggatttgaat cggggaagtg tgcgtacgca
     1261 aaacatcgcc gttttcaacg taaaacgtat cctgcatcgc acgggcagga tggtttgcag
     1321 ggatgttcag ggcttggaaa ttgtgaaaat cgtcttcgat ttcaggcccg tccgccactt
     1381 cgaaacccat tccgtgaaag agttcgacca cacgttgcaa ggtcagggtt acgggatgca
     1441 ggctgccgcc ttcctgagcg cgtccgggca gggtaatgtc gagggcttcg gcggcaagtc
     1501 gggcttgcag cttggcttcg ttgagggcat cgcgtttggc attaaaagcc gtctgaaacc
     1561 ggtttttgca ttcattgata tgcgcaccta tggttttgcg ctcttcaggc gacatttgcc
     1621 ccaaagtttt cagaagtccg gtcaactcgc cggttttgcc aagataacgg gctttgattt
     1681 gttctagagc gttgaagtct tgcgcagctt ctactgcggc aatgccttct gcaacgatac
     1741 ggtttacatt ttccataata tcaagcctgt caattaatga tatgccacct gaaacaacga
     1801 ggccgcctgt atagcctgac atatcagact cttcagacga ccttattttc ttcacggcca
     1861 atccattgga taaattttct gtattttaac gcaaatttat ttaaaaaaat agactttaaa
     1921 gaaagaaaat caatcacata gaatttccgc aaacaaagaa aaaaggaagc cgcagcttcc
     1981 ttttcaattt tttggattaa gcagccaaag cagctttggc tttttcaacc aattgtgcaa
     2041 aagcggcttt atcgaacacg gccaaatcag ccaatacttt gcggtcgatt tcgatagagg
     2101 cgcgtttcag accgttcata aatttgctgt aagacaaccc gttttcacgc gcacctgcat
     2161 tgatacgaac aatccacaat tgacggaatt ggcgtttgcg ttggcggcgg tcacggtatg
     2221 cgtattgacc agctttcatt accgcctgct tggcaacgcg gtaaacgttt ttacgacgac
     2281 cacgatagcc tttggctaac gcgaagattt tttggtgacg ggcacgggcg gtaacaccgc
     2341 gttttacgcg tggcatattc taaactcctt aagcgtaggg taacatttta gcaacagaag
     2401 ccaaatcgcg atcatttacc atagaggtac cgcgcagttg gcgtttgttt ttggtggtct
     2461 ttttagtcaa gatgtggcgt ttgaacgcat gagcgcgttt cacaccgccg ttacccagta
     2521 ctttaaagcg ttttttcgcg ctagacttgg ttttcatttt aggcatggga aaactccatt
     2581 cgttatcgga taaggcatta gggggtttta aaacatcggt ttcaaacact tgaacccaca
     2641 atgccacggt tttttgccgc aattgaaaac ttactccgaa gcggcaatcg gagtaagcgg
     2701 aaaattatag ctttattttt tcttcggcgc aatcatcatc accatttgac ggccttccat
     2761 tttgggaaag gactcgattt gcgccacttc agccaaatct tcttttacac gttccaaaag
     2821 ttgcgcgccg agttgctggt gagccatttc acggccgcgg aaacgcaatg tcactttgac
     2881 tttatcgccg tcggcaagga agcggttgat gttgcgcatc ttgatttgat aatcgccctc
     2941 atctgtaccc ggacggaatt tgatttcctt aatttgcacc tgcttttggt ttttcttggc
     3001 ttcgtcgcgc ttcttggctt gttggtattt gtatttaccg taatccatca gtttgcacac
     3061 aggaggttta gcagttgggg aaatctctac caaatcgaca tcctgccctt cggccatagc
     3121 caaagcttca cgaactgaaa cgacaccaag ctgttcgcct gactcactga ttaaacgcac
     3181 ttctttggcg gtaatttcac cgttgattcg tgcttcgcgt tcttgagcga tgatgaatac
     3241 tcctataaaa attaatgatt gacgagggca tcagtgattt cttgctgcaa ttgcgcaatg
     3301 aaatcatcca aatccaaaga acccaagtct tccgctttgc ggcgtaccgc aactttgttt
     3361 tcttgtttct ctttatcgcc gataacgatt tgataaggga aacggtattg gctgttgtcg
     3421 cggattttgt aaccgatttt ttcattacgc aaatccaact cggcgcggaa gcctgccgcc
     3481 tgcaatttgg cagcaacttc ccgacaataa tctgcctgat tttcggtgat gttcataatc
     3541 accatttgaa ccggtgcaag ccataacggg aaagagcctg catggttctc aatcagaatg
     3601 ccgacaaacc gctccaaaga acctaaaatg gcgcgatgca acataacagg ccgcgctcgg
     3661 tcgttgtttt ctgttacata ttctgcatcc aagcgttccg gcaagacaaa atccagttgt
     3721 aatgtaccgc attgccaaga acgacccaag gcatctttga cgtgatattc gattttaggc
     3781 ccgtaaaacg caccctcgcc cggcaattcg ccccattcca cgccgcaggc agtcaatgcc
     3841 tcgcgcaaac cctgctctgc cttatcccac acgtcgtctg aacctgcgcg tttttcaggg
     3901 cgaagggaaa gcctgacgga tacatcatgg aaaccgaact gtttgtagat gcgaaccaac
     3961 aattcattga acgcacgagc ctcgctgacg atttgatctt cggtacaaaa aatatgcgca
     4021 tcatcctgca caaaaccgcg aaccctcatc agaccgtgca gtgcgccgct cggctcgttg
     4081 cggtggcaag aaccgaattc cgccaaacgc atcggcaaat ctcgatatga acgcaaaccg
     4141 ttgttgaaaa tttgaacatg acccggacag ttcatcggtt taaccgcata ttcgcgtttt
     4201 tccgaactgg ttacgaacat attgtctttg tagttgtccc agtggccgga tttttcccaa
     4261 aaggttttat ccataatttg aggcgttttg acctctttat aaccggcggc gttcagctct
     4321 ttacgcatat gctgctcgat agtctgccac aaagcccaac ctttgggatg ccaaaacacc
     4381 atacccggcg cttcgtcctg caagtggaac aaatccagtt gcttgcccaa tttgcggtgg
     4441 tcacgttttt ctgcttcttc aatgcgttgg atatagtctt ttaattcgtc tttcgtcgcc
     4501 caagcagtgc cataaatacg ttgcagcatt tcattattgc tgtcgccgcg ccaatatgcg
     4561 cctgccagct tggtcagctt gaagtttttc aggaagcggg tgttcggaac gtgcggaccg
     4621 cggcacatat cgacatattc ctgatgatga tacataccca ttgcttcaac ttcgggcata
     4681 tcttcaatca ggcgcagttt gtattcttcg ccgcgctctt gaaaaatttt aatcgcctcc
     4741 gcacgcggag tcatgatttt gaccacatca taatcttggg caatcaattc tttcatacgc
     4801 gcttcaatgg cggcaacatc ttccggtgta aacggttttt ccgtggcgat gtcgtaataa
     4861 aagccctctt caatgacggg gccgataacc atttttgcat taggatagag ttgcttgacg
     4921 gcatgcccga caagatgtgc gcaggaatgg cggatgattt cgatgccttc ctgatctttc
     4981 ggagtaatga tttgaacagc agaatcctca acaatcgggt cgcacgcatc gaccaatttg
     5041 ccgtttacct tgcctgccac cgccgccttc gccaaaccgg caccgataga cgcagcaatt
     5101 tgagccacgg taacggggga ttcgtattgg cggactgaac agtccggcaa ggtaatattc
     5161 aacatcaaac gctccgaaag attaaaaaat aacggcataa atgccgttat gggaatttgg
     5221 taggcacgat tggattcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac
     5281 tgagctacgt gccttcaaag aatttttgta ttttatcggg tcgttttaat ttttgcaaga
     5341 gagatgtgca tttctttatg caacattgtg gcaagatttg gggataccgt ctgaaaaacc
     5401 gtgggaattc atttataatg ccgtctttta aaatatattt cgacccctgt cgcccaactt
     5461 acattcaatc ttatgcttaa atttacttta cacaaaaaag acggtctcgc ccgacgcggc
     5521 acgctggagt tgaaccacgg aaaaatcgaa acgccggttt tcatgccggt cggtacttac
     5581 ggttcggtca aagcgatgaa cccgcaaaac ctgcacgaca tcaaggcgca aattattttg
     5641 ggcaacactt atcatttgtg gctgcgtccg ggtttggagg tcgtcgaaca gtttggaggg
     5701 cttcacggtt ttatcggctg ggacaaaccg attctgaccg actcgggcgg ttttcaggtt
     5761 ttttctttgt cggatatgcg caagctgacc gaagaaggct gtacgtttaa aagcccgatt
     5821 aacggcgaca agctgttttt atcacctgaa atttccatga aaatccaaac ggttttgaac
     5881 tcggatatcg cgatgcagtt ggacgaatgc acgccgggcg aaacaacgcg cgaacaggcg
     5941 cgaaaatccc tgcaaatgag cttgcgctgg gcggaacgga gcaaaaaagc ctttgaagat
     6001 ttgaaaaacc cgaacgcgct gttcggcatc gtgcaaggcg cgatgtatga ggatttgcgc
     6061 gaagaatctt tgcgcggttt ggaggaattt gattttcccg gccttgccgt cggcggtttg
     6121 tccgtcggcg aacccaagcc cgaaatgtac cgtatgctgc acgccgtcgg cccgattttg
     6181 ccggaacaca agccgcatta cctgatgggc gtaggcacgc cggaagacct cgtgtacggc
     6241 gtggcgcacg gtatcgatat gttcgactgc gtgatgccca cccgcaatgc gcgcaacggc
     6301 tggctgttta cccgtttcgg cgatttgaaa atcaaaaacg ccaaacacaa gctcgacaag
     6361 cgtccgatag acgaaagctg cacctgctac gcctgccaaa atttcagccg cgcctacctg
     6421 caccatctgc accgcgccgg cgaaatcttg ggcgcgcaat tgaacaccat ccacaacctg
     6481 catttctacc aagtcattat ggcggaaatg cgcgaagccg tcgaacaagg caaatttgcc
     6541 gactggcagg cgcaattcca cgaaaaccgt gcgcgcggcg tggactgatt cccggcaatg
     6601 ccgtctgaag ccgcttcaga cggcattttg ccgccttacc ctacctgcac tatttttcag
     6661 aaaccgatca tgcttccatt ccttccagaa ccctcccttt cctatacgca acaaaaccgt
     6721 accgccgttt tgctgttgaa cctcggcaca cccgacgcac cgaccgcaca ggcggtacgc
     6781 ccttatctga aatcctttct gaccgaccgg cgcatcgtcg aactgcccaa atggctgtgg
     6841 tatcccattc tgcatggcct ggtactgacg ttccgcccca aaaaaagcgc gcacgcctat
     6901 gaaaaaatct ggtttaagga aggttcgccc ctagaagttt acacagcacg ccaagctgcc
     6961 gcgcttgccg aacgtatgcc cgacctcatc gtccgccacg ccatgactta cggcaatcct
     7021 tcgatagcgg atgtattggc ggagctgaaa tcccaaggtg taggcagatt gcttgcgatt
     7081 cccctgtatc cgcaatatgc cgcttccagc agcggagcgg cggtagataa agtgtgcgaa
     7141 caactgctgc tgcaacgcaa ccaaatgagc gtccgcacca tttcgcgctt ttatgatgat
     7201 gccggctaca ttgatgcgat gaaaaaccac atcctccgat attgggcgga acacgggcgt
     7261 gggaaaaaac tgatgttgag ttttcacggc gtaccgcaga agcactacga cctcggcgac
     7321 ccctatcccg acgagtgccg ccacaccgcc aaactgcttg ccgaagcact ggaactgacc
     7381 gaagacgaat ataccgtatc gttccaaagc caattcggca gggcaaaatg ggttacaccg
     7441 agcacgcagg atttgttcgg caaactgccc aaacaggggg taaccgagct ggacgtattc
     7501 tgcccgggct ttttggcaga ctgcttggaa accatggaag aaatcgccct gatggggcgg
     7561 gaacagtttt atgaagcggg cggaaaaaac taccgctaca tcccctgcct caacgacaac
     7621 cccgactgga tagacgcact cgtcgcactt gccgaagaaa accttggcgg ctggcgttga
     7681 aactgtttga gccatgtcta aaatgccgtc tgaacgccgt gttcagacgg cattttcccg
     7741 ctactttagc gtatgatggc atctatgccg gacagcacat caagtgcaac cttactaccg
     7801 attcggcaaa aaaacctttc acccgctacg caaaatcaac aacaaggttt caaacattaa
     7861 ggaaacgcca atgaacacaa cccgactgcc gaccgccttc atcttgtgct gcctctgcgc
     7921 cgccgcttct gccgccgaca acagcatcat gacaaaaggg caaaaagtgt acgaatccaa
     7981 ctgcatcgcc tgccacggca agaaagggga agggcgcggc actgcgtttc ctccgctttt
     8041 ccggtcggac tatattatga acaaaccgca cgtcctgctg cacagcatgg tcaaaggcat
     8101 caacggtaca atcaaagtca acggcaaaac ctacaacgga ttcatgcccg caaccgccat
     8161 cagcgatgcg gacattgccg ccgtcgccac ttatatcatg aacgcctttg acaacggcgg
     8221 cggaagcgtt accgaaaaag acgtaaaaca ggcaaaaggc aaaaaaaacc aaacagacaa
     8281 aatgccgtcc gaaaccggca atccggcttc agacggcatt caaatcaaac ctttctaacg
     8341 cggtactttc agccccatag acccgatatt gccgccaccg tcaacctcct tcgcggtcag
     8401 cataaagccg ccgatgtcaa tacggtcgcc ctcgaccggc tcctgactgt cggaacgctt
     8461 atcgaaaagc tcggcaaggc tcaaaccctc ttcgcccgct tccagcttca aaccgtaagc
     8521 aagtgccaaa tcgcccgaac gtgctgccgg cgaaacgaca aactcaccga agaaatcaaa
     8581 attttcacgg acgccgatac ccgtctcggt aaagtatttc gccattttat cgaccttgcc
     8641 gtcgggcagg atgtaccacg ccaaatcctc cgcctgaagc acggtgtcgg actgcatttc
     8701 gattcggctg ccgttgcgta tcaatgcgaa acagcgtgcg tcaaacgaat cggaaatcgg
     8761 ttcgaccgta tcgggatggt gtccctccgc ttcagattcg gcaaccactt taaacgccga
     8821 cattctgaca atttcctttt ctgccagcca aatatcatgc gtatctttcg gttcgggctt
     8881 gttgggcatg gcaaccttca acagccgcgc catcacagga atcgtcgttc cctgaatcag
     8941 cagcgacagc accaccacgg caaacgccac atcaaacagc aggtgcgaat tgggaacgcc
     9001 catcaccagc ggcatcatcg ccagcgaaat cggtacggct cctcgcaagc ccaaccaact
     9061 gatatacgcc ttttcacgca ggctgtaatt gaatttccac aaaccgccga acactgccag
     9121 cggacgcgcg accagcatca ggaacgccgc aatcgccaag gcttccgccg ccctgtccaa
     9181 cacgccggcg ggagaaacca gcagaccgag catgacgaac aaagttgcct gcgccagcca
     9241 agccaaaccg tccatcacac gcaaaacgtg ttccgtcgca cggttgcgct ggttaccgac
     9301 aatgatgccg gcaaggtaaa ccgccaaaaa gccgctgccg cctatggtat tggtaaacgc
     9361 aaacacaagc agcccgcccg acacaatcat cagcgcgtac agaccttccg caagattcag
     9421 acggcgtacc agctttgcca atatctttcc gcccgcccaa cccgtcagca gaccgaaacc
     9481 gatttgcagc gcaagcatcc ggacaaacgc tgccgcaccc gattccgccg gctgcataat
     9541 catggtaatc agtgccgtaa ccaaaaaaac cgccatcggg tcgttcgcac ccgattcgat
     9601 ttccaaagtc gcctgcaccc gttcgttcaa acgcacgccg ctgttgcgca aaaggctgaa
     9661 taccgcgccg gcatcggtcg aaccgacaat cgccgccatc agcacgccga acttccaatc
     9721 caaaccgaga taaaaagttg caaacagtcc cagcggaagc acagtggcaa acacgcccca
     9781 cgaagcgagt accgacgcgg gcttcaacgc aatccgaaaa ctggaaagct gcgtccgcaa
     9841 accgccgtcg agcagaataa ccgccaaagc aagctggctg atgaaattcg ccatcacgac
     9901 attattgaag gcaatgccgc caacgccttc ctcgcccgcc agcatgccga cggcaagaaa
     9961 caccagcagc aaaggcattc ccaaacgtgc ggacaaagtg gtcgaaacca cgctcaggaa
    10021 caacagtgcg ccacccagca gaaacaggct gttcatccaa tccacgccaa cggctccccc
    10081 ttaaaattca tgaaattaga atcgaaagat tgtaacacgg cattttcttc tcacgaaact
    10141 ttatttcatg tccgccaccg ccttcagacg gcattttcct gtttcatgcc gcctgatacc
    10201 gcccaaaccc gcgttctcct atataatgtc ggactttgcg ccgcatttca ggatttttta
    10261 tggcatttgc ctcactattt actctgccgg actacatcac cgccgttttg gacgatgttg
    10321 ccctgatgac caaagccgcc gcaaagaaaa ccgccggcgt ggccggcgac gatttggcac
    10381 ccaatgccaa ccgggttaca ggcgtatcgg cagaacgcga gttgccgatt gtttggccgg
    10441 tggcgaaagg ttcttttgcc aacaagccgg ttttggtgtc tgcggctctg ctgctttccg
    10501 ccttcctgcc gcaactcatt acgccgcttt tgacggcagg cgggatttat ttgtgctttg
    10561 agggcgtgga aaagctgctg cacaagtttc tgcaccgaca tgacgcgcac gatgacggcg
    10621 gcgaaacggc ggttaacgag caggctaaaa tcaaaggtgc aatccgtacc gactttatcc
    10681 tgtccgcaga aatcatcatc atcgcgctgt gcgtggtcga acaatacagc ctgatgacgc
    10741 gctcgctcgt gatggcggca atcggcatcg gcatgactgt tttggtgtac ggaatcgtcg
    10801 ccgtcatcgt caaactcgac gacttgggca tgctgctgat gcgccgccca caacctcggc
    10861 cttttgcacg atttcctgca cgcgcaacac tggaacgctg gctggacaga gtattccgcc
    10921 aacctcgttg tcgggccgct ttccggttcg attgcctgcg ccccgccttg tcgctgatga
    10981 atcgtttcgg caggcattga ttcctttttc aaataccgat gccgtctgaa agacgttcag
    11041 acggcatatt ccgaacagac agacgaatat gttttccaaa ctggacaaat actggcagca
    11101 ccccgccctc tactggcctt tgctcatcct ttttgccgcc gccaccccct tcgccttcgc
    11161 accctactac cacttttggc tgatgccctt gattttcggc gccttcgtcc gcctcatcga
    11221 actgcgtccg cgttttgccg cctcttccgc ctacctgttc ggtctgaccg catacacggc
    11281 acagttctac tggatacaca ctgccctgca cgacgtttcc ggcctgcccg acctctatgc
    11341 cgtaccgctg accttcctgc tgcccgccta ccttgccctt tatccggcac tgtgtttctg
    11401 gctgtggaaa aaatttaccc tgccccaggg cataaagacc ggtctggtac tgcccatcct
    11461 gtggacgctg accgagtttg cccgcgagcg tttcctgacc ggattcggct ggggcgcaat
    11521 cggctactcc caaatcaccc cggacagccc gctggcgggt ttcgccccct tgggcggcat
    11581 ccacatggtt acactggcaa ccgcctttct cggtgtctgg ctggttttgg cgatcgacaa
    11641 caccgcacgt tcgggcaaac gcctgcttcc ggccattctg attgccgccc tgcttgccgc
    11701 aggctacacc gcccaacaaa ccgacttcac ccgccccgac ggcagccgca gcaccgtcgc
    11761 cctgcttcag ggcaacatcg cccaaaccct caaatggcgt gaagaccaag tcatcccgac
    11821 catacagaaa tattacgaac aggtcggcaa aaccactgcc gacatcgtca tcctgcccga
    11881 aaccgccatt cccatcatgc gccaaaacct gccggaaaac atactggcgc aattcgccga
    11941 acaggcgcaa aacaacggca gcgcgctcgc cgtcggcatc agccaataca cttcggacgg
    12001 caacggttac gaaaacgccg tcatcaacct gaccggctat caggaaaaca atcaggacgg
    12061 catcccttac tacgccaaaa accacctcgt ccccttcggc gaatacaaac cgctgcccct
    12121 cctgaccaca ccgctttaca aaatgatgga catgcccctt tccgacttcc gcaaaggcgg
    12181 cggcaagcaa tccgccatga tgatgaaaaa ccaaaaaatc gccttcaaca tctgctacga
    12241 agacggattc ggcgacgaac tgattgccgc cgccaaagac gccacactgc ttgccaatgt
    12301 cagcaatatg gcgtggtatg gaaaatccaa cgccatgtac cagcacctcc aacaatcgca
    12361 ggcgcgggct atggaactcg gacgctatat ggtccgcgcc accaacaccg gcgcaaccgc
    12421 catcatctcc cccaaaggca acatcatcgc ccaagcccaa cccgatacgg aaaccgtatt
    12481 agaaggacac atcaaaggct acatcggcga aacgccctat atgaaaaccg gcagttcatg
    12541 gtggttgatg ggcatattga ccctagccgc actgattctt ttcatcttcc gaaacaaaga
    12601 acactgatac ctgcacagtc cgtccggacg ggctgtcttt tttcaaaccg gccgtttaca
    12661 tacaaccacg tatgtaatat aattctaaaa tcgaattgag ctatcaggtt tacaaaatca
    12721 atcaaacttg ccttacaaaa ttttcctgtc tttcaaacct tagcgtatat ctcctgccaa
    12781 acatacaaaa tacaagccaa gcagtgatat actgcatccc aacacaccaa accgccgata
    12841 cgcagtttca gccgaaagga aatgccccaa atgaataacg ctttcgcatt acccgccatc
    12901 caaagcggca acggcagcct cgaacaatac atccacaccg tcaacagcat cccgatgctg
    12961 tcccaggaag aagaaacccg cctcgccgaa cgccggataa agggcgacct caacgccgcc
    13021 aaacaactca tcctgtccca cctgcgcgtc gtcgtttcca tcgcgcgcgg ctatgacggc
    13081 tacgggctga atcaggccga cctgattcag gaaggaaata tcggtctgat gaaggcggtc
    13141 aaacgctacg agcccggtcg aggtgcacgc ctgttttcat ttgccgtgca ttggattaaa
    13201 gcggaaattc acgaattcat cctgaggaac tggcgccttg tccgcgttgc caccaccaaa
    13261 ccgcaacgca agctgttttt caacctgcgc agcatgcgta aaaaccttaa tgtcctgtct
    13321 cccaaagagg cacaagacat cgccgacgat ttgggtgtca aactttccga agttctggaa
    13381 atggaacagc gcatgacggg acacgacatc gccatcatgg cagacaacag cgacgacgag
    13441 gacagtttcg cccccatcga ctggcttgcc gaccacgatt ccgaaccgag ccgacaacta
    13501 tcgaaacagg cgcattacgc cctgcaaacc gaaggcctgc aaaacgcctt ggcgcaattg
    13561 gacgacagga gccgccgcat tgtggaaagc cgctggcttc aagacgacgg cggactgacg
    13621 ctgcaccagc ttgccgccga atacggcgta tctgccgagc gcatccgcca gattgaagcg
    13681 aaagccatgc agaaactgcg cggtttcctg accgaagaag ccgaagcggt ttgaaatata
    13741 cacaataaaa tacccgacaa aacaatttgt cgggtatttt atttggtaaa catcaatgcg
    13801 gtttgtgcgt cgtgccgtac acgattttat ccgcccaagc catcgcaatc gccaaaatca
    13861 aaaagcagac atgcaccaga cactgccaca tcagctgctt ttccgacaga ttggcggaat
    13921 tgataaatgt ttggagcaaa tggatggatg aataccgata atcgacatcg acagccttac
    13981 cttcaatacc ggtgcattca catggctcaa ccactcgggc cggtcaggat ggtcgtcgat
    14041 acgcaaccgg gatacgaacg actcataccc gccaatctgc accatggtca gcaaattcgc
    14101 aatcataacc acatcaatca gatttaaaac agcgagcatg atggcgtttt cgtccgacac
    14161 atccaaattc atgaccagat gcttcaacga tttcaaaaac ttataggcac aaatcgcccg
    14221 tacaacagtc agccccgcat aaatcggcac ttgcagccaa cggcttgcaa aaatcatttt
    14281 ggcaaaaaac ccatagcctg ttcgggtttt ggagattttg cttgatacat ttcccttcct
    14341 tgcagcaaca aaagcgcgtg attccaatct accactgcga aatcttcaat ttttcacacc
    14401 cgaaaacgct tgccgccata cagatgtgat atattgattc cattttaacg aacttcggag
    14461 aacgacatga gcagcggaaa tatccgcatc cacagcatga ccggctttgc caacgcggcg
    14521 gcagagtgcg gcagcaaacg catcaacctc gacatccgcg ccgtcaacca ccgctttttg
    14581 gacattcaaa tcaggatgcc cgacgatttg cgccatttgg aaaacggcat ccgcgagcag
    14641 atttcatccc atctggcgcg cggcaaagtc gaatgcaaaa tccaaattca agatgcggaa
    14701 aacggcaatc agtctttgga actgaatcgc gttctggtcg gacagcttgc cgaaatcaac
    14761 aaaaacctcc gcaaacatta tgatttggca aaactgggcg ttgctgatat tttgcgcttt
    14821 ccgggcgtat tggcaagcca aaaggaaaat acggaagcat tggcgaaaac cattgtcgaa
    14881 ctggccgaca aagcactaaa agactttacc gccgcacgtc ggcgtgaagg gaaaaaattg
    14941 ggcgaacacc tgttacaacg cattaaaaac atggaagaaa tcatcgatgc tttgagcgaa
    15001 atcttccccg cgctggtgga agcgcataaa gaaaaaatcc gcacccgcct tgccgaagcc
    15061 gtcggcagca tcgacaacga caggctgcaa caggaatttg ccctctttat ccaaaaatcc
    15121 gacatcgacg aagaattcag ccgcctgcgc acccacattg ccgaagtgcg ccgtatcgtt
    15181 accgaacaca aaggcagcag cggcaaacgg ttggacttcc tgatgcagga actgaaccgc
    15241 gaagccaaca ctttgggcag caaatccatc gctaccgaat gcacccaagc ctcggtcgag
    15301 ctgaaagtcc tgatcgaaca gatgcgcgaa caggtgcaga atatcgaata accctgcctt
    15361 tctctccaaa catgacaatg ccgtctgaaa cttttttcag acggcattct tgatcggctg
    15421 aaaacctctg ttgcaagcaa ttcaagattg ttctttcaca tccaccaacc tcgttccggc
    15481 aaggaagtca tacagaaact gccggtcggg attcagcagg gcgaaccccc acggcaaaat
    15541 cagccaaacc aatgccgcgc cggccgcgcc cttgggcgga atgccaagga agtggcgcaa
    15601 tccggcataa gcaagcatag gaatgaagac gatgaatatg cacgcccaaa taaagcgcag
    15661 gcgcagcagg tgcaaagacg gctgtatgcc gttaaggtcg cacaagccga ttttccatgt
    15721 cctcatcgcc aaggtctgcc cctgaccgtg ccagttggcg cggaaataaa gccaccatgc
    15781 tcccattatc aggatactcg ttaccaatgc agaaaccgcg atagaaacgg gattcagaaa
    15841 aatggcggca ataccggcaa gcaatgctgc caaacaggtc gccgcaccga ccagcagcat
    15901 ctcatacagt atggcggcaa agcggcgttt gagcggggcg gttttcagta tcatcatctt
    15961 ttcaatcgga atatgccgtc tgaaacgcca acagacttca gacggcattt gtgcaaagat
    16021 gccttgcggc ggttaacggt gcggacgttt ctgaccgcgc gactgttttg ccgccatttg
    16081 attgatttcc tgacgtgtcg ggcgtttttt gaacatagcg tgcgcctcgt ccaccgaaca
    16141 tttttgcgac aggcagatca gggtaatcgc atctttctgg tgttgtccgc cgctgtggta
    16201 catacggcgc aggtttgcct cgttgattct gcctttttcg ggatcgatgt cgaatttttt
    16261 cagacgttcg ataaactggc gttgctgtcg gcgggatgtc caaacaaagc cgcccgcagc
    16321 ggcggcgacg atgacggcgg caagcaggat ggcaagcggc caggaatcga caatcaatac
    16381 gataatgagg cttaaaacgg cggtggtcag taaaatcagg ccgcccaagc gcagtttggt
    16441 cttattgtcc atagggttga tgttccttta ttgaggtggt cgtgccgtct gaaatgtttt
    16501 cggacggatt ggtgaatact tttcaggatt aacggtattt tacagtattt tcggattttg
    16561 gggattaccg ctttattccg ccagcgacac gacaagctgt ttgaataccg tccatgcgtc
    16621 gccgtcttcc gcgcctttga tgattcggtc gatttgggcg caggttttga gcgcgtcaag
    16681 caggcggacg acggaaatcc gcttgaccgc aagcggtgcg agcgtctgtt tgtcgcccca
    16741 aagcctgagg ctgttgcgga cggattggat gctctgcccc tgtttcaggg cagcggcaag
    16801 ccgaatcagc gtccgcacgt cttcggcaac cgcccacaga agcagcaccg gttcttcgcc
    16861 ttcttcctcc aacccgtcca acagcctgca tacgcgcggg acatcgccct tcatccacgc
    16921 gcctgccagt tggaacgcgt cgaagcgggc aacattggca acggcggttt gcgcctcatc
    16981 gatattgacg gcatgccctt tcggatacag cagcgcgagc ttgtcgattt cctgacgtgc
    17041 cgccaacaga ttgccttcca cgcgctcggc aaacagtgcc aatgcgtctg cctcgatacc
    17101 caaaccgatt ttgtccagcc gtccgcgtat ccattggggc aaagccgccg caccgaccgg
    17161 tttggcttcc cacacttccc cctttgccgc caatgcggca aaccatttgg actggagccg
    17221 ggttttctcc agtttgggca gcaaaaccag cgttaccgta tcttccggca gtcgggcggc
    17281 aaaatcctgc aatgcctcgc cgccgttttt gccgggtttt ccgttgggga tatgcaattc
    17341 caacagtttc aaatcggcaa acagacccgc attgcctgcg gtttgcagca gttcgttcca
    17401 atcgaaagac gcgtcggcgg tataggcttc gcggttgaga taaccctgct tcttcgccgc
    17461 cgccctcaat gcgtccactg cctcgatacg cagcagttct tcctcgccgt ggatgacgta
    17521 cagaggtttc aaaggcgcgt ccgtatcgat gcgtccgata tgtgccgcca cgtcattccg
    17581 ccttcagaaa ggtcaggcgg cggacaatct gttcggcaac atcctgccgc atttccgccc
    17641 acagggtttc ttcttcttcc tgtttgccca aaatttcgtt gtcggcataa tccaaaatgc
    17701 ggcggacgga cacggtcatc ggtttgccga ccggctcgcc gcgtttcaat acctgcgctt
    17761 caaccgtcaa tatcaaaaga tattcgttga tgactgccgc acgggtaacg gtataggttt
    17821 ccttgttttg ggaaacgctg tctatacgca gggtcatctg cgcgccggca gcatcgtcca
    17881 ccctgcccga agcctgatac agcgcggttt ccaaaggaaa ctgcaatgcc tgtccgcctt
    17941 cgatgtgcca gctccggtag gtcagcggcg gagaaatgcc gtctgcacct ttcaggtgga
    18001 aaccgcacgc gcccagcacc aaggctgcgg cagtaaggaa tattttgttc atcgcttgcc
    18061 tttctcaaaa acataaagtc ggtagattat atcagcggcg tgttcagacg gcattgccgt
    18121 ccagccaaat caggctcgcc atacgccccg tcgctccgtc gcggcggtag gaaaagaaaa
    18181 tatcccgttc caaaaccgta caatgcgtgc cgccgtacac gccgcccacg ccttcgcgcc
    18241 tcagaatcag gcgggcgagc gcgtagaggt cggcaaggaa tttgccgccg ccgatgggtt
    18301 caaatgcagc agcggcttcg ggcatggacg tgcagaacgc atcaaacacg tcctgcccga
    18361 cttcaaacgc atccgcactg atggcagggc cgagatacgc cataatttcg acaggcggaa
    18421 ccttcatcgc ggcaatggtg ttttgcaata cgccgcccgc caaaccgctc cagcccgcgt
    18481 gtgcggcggc aaccgccgta cctgccctgt cgcaaaatag aacgggcaga cagtctgcag
    18541 tcatcacggc acagacggct ttacccgtgt cgtccacgga agcgtcggca tcgggcgtgc
    18601 cgtccaatgc ttcggcagca ttgacgacaa tggtgctgtg gatttgattg agataggcaa
    18661 cgggcaatcc gacctgctgt tgcacgattt cgcggttgcg gcgcacggtt tcagggtcgt
    18721 cgccgacgtg cgtaccgagg ttcaaactct gatacgcacc ttggctcacg ccgccgttgc
    18781 gcgtggtaat cagggttttc acattggcgg gcgcgggcca atcggcggtc aggaagtttt
    18841 tgcctttcgg ggcgagattt agggtttctg tgatggtttt cattggatat tggaataggt
    18901 gtgcggcgga aagggttgat taactgcctt cggggcaaat ggatgcttgt tttttcgtca
    18961 ttcccgcaca ggcgggaatt cggagattcg ggattgtttt caaacgttcg ggtcggattc
    19021 ttgccggtgc gggaatgatg cccttatata ctttctccga gctttatatg cttcaacagg
    19081 ggacggcaca tcaagcaccg cactgcgtgt tgcccgaacg aggctgccct ccgattagat
    19141 tctatcgcta taaacagacg ggtttcaacc gaaaaggaat ggggataaag tccatttccg
    19201 acacctctcg ggctataccg tctgaaaacc aatctccact ttcagacggc attgtttagg
    19261 cgcggtcatt ttgcaaatta aggttaaggc gtgtatgctt gccttcactt aaaaacaaac
    19321 acgcaacgca ttattatgaa tatcctcagt aaaatcagca gctttatcgg aaaaacattt
    19381 tccctctgaa cctcgctctt tgccgccgcc gcttttttcg cgcccgacac cttcaaatgg
    19441 gcggggcctt atattccttg gctgttgggc attattatgt tcggtatggg tttgacgctc
    19501 aaaccttccg acttcgatat tttgttcaaa catcccaaag ccgtcatcat cggcgtaatc
    19561 gcacaattcg ccattatgcc ggcaaccgcc tggctgccgt ccaaactgtt gaacctgcct
    19621 gccgaaatcg cggtcggcgt gattttggtc ggctgctgcc cgggcggtac ggcttccaat
    19681 gtgatgacct atctggcgcg cggcaatgtg gctttgtcgg ttgccgttac gtctgtttcc
    19741 accctgattt ccccattgct gactcccgcc atcttcctga tgcttgccgg tgaaatgctg
    19801 gaaatccaag cggccggtat gttgatgtcc atcgtcaaaa tggttttgct ccccattgtt
    19861 ttgggtttga ttgtccataa ggttttgggc agtaaaaccg aaaagccgac cgatgcgctg
    19921 ccgctggttt ccgttgccgc catcgtgctg attatcggcg cggtagtcgg ggcaagcaaa
    19981 ggcaagatta tggaaagcgg cctgctgatt tttgcggttg tcgcactccg caacggcatc
    20041 ggctacctgc tcggcttctt tgccgccaaa tggaccggcc tgccttatga tgcacaaaaa
    20101 acgctggcca tcgaagtcgg tatgcaaaac tcgggcctgg ccgccgcgct tgccgccgca
    20161 cactttgccg ccgcgccggt cgttgccgtt ccgggcgcat tgttcagcgt gtggcacaat
    20221 atctccggct cgctgctggc aacttattgg gcggccaaag ccggtaaaca taaaaaaccc
    20281 taaacgcctt tcatcataat gccgtctgaa cggggcaacc gaaaccggcg gtttctgccc
    20341 ggccccttca gacggccttt gtttgaaatc ggcggggacg attgcccggc tgctccggtt
    20401 cggctgcctg cgttcgcccc gcctttcaag ccgcctcact cccttacata aaccacttcg
    20461 acatcgtagt cgtcttcgtt ccaatcgtca tcgtcgtccg cgccgaattt gtcctgccat
    20521 tcctcttcat tgctcaaaga cgaatccaaa ccggcttcaa gacggaggac ggacaacaaa
    20581 tgatatatgt cgtccggaat cggtgcctca aacgaaacgg tttcgccgct ttccggatgg
    20641 gtaaaactca agcggtaggc gtgcaacgcc tgacgcgcgc ccaaactttt aacggcttct
    20701 ttcaccgtgt cgccgcacgg atggcgcggg ttgccgtaaa ccgggtcgcc ggcaagcgga
    20761 tggttggcct cgcgcatatg gacgcggatt tggtgcgtcc tgcccgttcc gagcgagcat
    20821 tcgatgtagc tgtgggcaag atagcgttcc aacactttga cgtgggttac ggcaggcttg
    20881 ccgccgaatt tgacggctgc cattttcagg cggttgtgcg gatcgcgtcc gatttgggtt
    20941 tcgattttac cgtcgaaggg gacgatgccg ttggcgacgg cgcggtagat gcgtttgacc
    21001 gtgcgttctt gaagctgcct cacgagggaa ttttgcgccg gcagggtttt ggcaaccacc
    21061 atcagcccgc tggtttcttt gtccaaacgg tgtacgatgc ccgcgcgcgg aatttgggac
    21121 agctcggggc aatgcgccaa caggccgttg agcagcgtcc ccgtccagtt gcccgccgcc
    21181 ggatgcacca ccagtccggc cggtttgttg acgacgatga cggtatcgtc ttcgtaaaca
    21241 atatccaaat ccataggctc cggaacaaac gccagatttt cctcactcgg acggacggtt
    21301 acacaaattt gctcgccgcc tatcattttg tctttgggtt gcgaaggttt atcgtttaca
    21361 ataaccgcgc cttctttaat ccatgatgtc aggcggctgc gcgaatagtc gggcagaagt
    21421 ttcgccaaca ccgcatccaa ccgcccgccg gcaagctcgg gcggaacggt cagaccgata
    21481 cggttttcag ttccggaagc tgacgcaaag tctgaatcgt cgctataatc ggattcatta
    21541 tcaaaggaag tattctgcat gaaaaaaatt cttttaacgg tttcattagg tttggcactg
    21601 agtgcctgtg ccactcaagg tacggccgac aaagatgccc agattaccca agattggagt
    21661 gtggagaagc tctatgccga agcccaggac gaattgaaca gcagcaatta tacgcgggct
    21721 gtcaagttat acgaaatctt ggaatcgcgc ttccccacca gccgccatgc ccggcaatcc
    21781 caactggata ccgcatacgc ctattataaa gacgatgaaa aagacaaggc tctggcggca
    21841 atcgaacgct tccgccgcct ccatccgcag catccgaaca tggattacgc gctgtacctg
    21901 cgcggcttgg tgctgtttaa cgaagatcag tccttcctga acaaactggc ctcgcaagac
    21961 tggtccgacc gcgacccgaa agccaaccgc gaagcgtacc aggcgtttgc ggaactcgtc
    22021 caacgcttcc cgaacagcaa atacgccgcc gatgcaaccg cacgcatggt caaactggtc
    22081 gatgctttgg gcggcaatga aatgtcggtg gcgcgttact acatgaaacg cggcgcatat
    22141 atcgccgccg ccaaccgcgc caaaaaaatt atcggcagct atcaaaatac acgctatgtc
    22201 gaagaatcac tggccatatt ggaacttgcc tacaagaagt tggacaagcc ccagcttgcc
    22261 gccgatacgc gccgcgtatt ggaaaccaac ttcccgaaaa gcccgttttt gacgcacgct
    22321 tggcagcccg acgatatgcc ctggtggcgt tactggcatt aagcccatcc tcgaaatgcc
    22381 gtctgaagcc ctttcagacg gcattttccg ttccggcacc agccttcccg ttcactcaaa
    22441 cggttttttc tgccaataga atttatacac tctcaaacgt tgagccttta cgcctccgcg
    22501 tcccaagccg aattgcagcg cgcccgacaa atccgtccgc aacaacttga tgccgtgtgc
    22561 gcggacacgg ttctgcaccg cttcggtcgg atgcttgtag gcgttggcat aaccgcttga
    22621 agcaacggca tattcgggtg aaacggcatt gaggaacacg cccgacgagg acgtattgct
    22681 gccgtgatgc cccaacacca acacctggct gtacaggttg cctccatact tgccgaccag
    22741 gctttcctcg cccttcgtat ccaaatcgcc cgttaccagc agtgccgcac cgcccgccac
    22801 aacacgcaaa acacaacttt tcccattatc atcgatattt ttgcgttcag acggcctcaa
    22861 aaactcgaaa tccacgccgt cccattgcca acgctgttcc gcacaatgcc gcgccccctc
    22921 ataaaattcc ggctgtccgg cataaatccc gccgttcggt atcttgccga ctgcctgaaa
    22981 gccgccgtcg tggtcgctgt cgtgatgcga cagaaccagc ttgtccaaac ggcggacacc
    23041 cgccgcattc aaactcggca cgatacccgt ttgtgccgcc gctaccgttc ccgtatcgaa
    23101 aagcagatgg cggtttgccg tccgcacaag caccgacaag ccctgccccg catcccaaac
    23161 cgtaaccgca gcttcgtttt caggtactgc ttcaggacgg taggatacaa accctgccaa
    23221 cagcaacacc gcccacggac gcaaacccaa gccgcgcggc agcagcaaca gcaaagcggc
    23281 acacaccgcc aacaccagca gcggcaaagg tgcggcggca acggcaaact caggcgacac
    23341 atcggcaagc cacaccaaaa accgcaaagt gtattccgcc aaaaatgccc ccgcctgctg
    23401 aagcggcgca aacggcacga ccgaacccag caacgccagc ggggtcaaca cccaagaaaa
    23461 ccacggtatc gacaccgcat tgaccaaagg gctgaccaaa ggcagcgagg caaacagata
    23521 accgagcaaa accagagaca acaccgaagc cgcccactgc ccgcgcacgg cggtttgccg
    23581 tttcccctcg tacaaacgcc ctgcacacgc ccatatcagg gccgccacca aaccgaaaga
    23641 cagccaagta ccgacaccca aaaccgccaa agggtcgaac agcagcaccg ccgccaacgc
    23701 ctgccaccac gtcgcccacg ccgacagtct tcccctgcgc cacgcccacg cgaacgccgc
    23761 caacatcaaa acactgcgct gcgtcggcac ggaaaaaccg gcaagcagcg cgtaaaacag
    23821 cgcgcccgca caccccgccg ccaaaaccca cgcgcgcggc cgggcgggaa ggcgcgggga
    23881 acaggcaagc agccgcttcg ccagccacgc aaacagcacc gccaccatcg taacgtgcaa
    23941 acccgagata ctgaccaaat gcgtcagccc caacggccgg aacgcctgcc acaatccggg
    24001 gcgcaatgcc gactgttcgc ccacgctcaa cgcgcgcatc agcccgatgc cgtctgaaag
    24061 cccgccgtcc gcatccgcct gccgccaatt gcggctgatg cggctgcgcc aaaccgcaat
    24121 cccccaaccg cttccgccat gcagcaaaac cctgtccgca ccgaccgtcc ccacgccgcc
    24181 taccccgttg gataatgccc acgcctcacg gttcaaaccg cgcaggttca actccccgac
    24241 gacggggtgc acgcgtgccg ttatccgcca tctgctcccg accgcccatt cgcgccgttt
    24301 gtagtccgac agcagcaaat caaacgtccg accgccgctg tccacagcct ttgccgcaaa
    24361 ctgcacgcgc cgcccgtccg acctcggcat atccgtcact tccaccgtca aaggcacgcc
    24421 tgaaaccgcc tccgcccgcc attgcgaaga cagtgccgct tccgttctga atacgccgta
    24481 agccgcgccc gccaacacgc acagcatcag accggcaaac gcaaaacgcc ttgcaagcac
    24541 agccaaaacc gcaaaagccg ccaaccaaaa cggccaatgc ggtacaaccg acagtgcgaa
    24601 cgaaactgcc acgccgacaa cccaacacgg caaaaaccaa cgacggaaca cgtctcccca
    24661 ctcccgatat aaaaaacaaa cgatgataaa gcaaagccat aaaaccgtca taacaagatt
    24721 tgaaaatgat aagaatgcaa acgaaaagcc tcagtattat ccgagacaac ggcaaacttt
    24781 ccatcgtcat tcccgcaaaa gcgggaatcc aaagacaaaa agccgcagaa atttatcgga
    24841 aagaaccgaa ccacaaaaac cggcttccca tcttcgcttg aataacagaa caaagaaagc
    24901 ggaaataaaa acatcaggat tcttttttaa attggcaata aaacaccaaa atgaatgagt
    24961 tacacaaaaa aatactcaac accacccaac cggcgtaaaa tgcaaaacat tatcgctatt
    25021 aaaacggtaa aaccttatga aagccaaacg ttttaaaatt aacgccatat ccttatccat
    25081 ctttcttgcc tatgccctta cgccatactc agaagcggca ttggtgagag acgatgtcga
    25141 ttatcaaata ttccgtgact ttgcagaaaa caaaggcaaa ttttttgtcg gcgcaaccga
    25201 tttatcagtg aaaaacaaac aaggtcaaaa catcggcaac gcgttatcca acgtaccgat
    25261 gattgatttt agcgttgcag atgtcaacaa acgcatagca acggtagtcg atccccaata
    25321 tgccgtcagc gtcaaacacg ccaaagcaga agtccatact ttttattacg gccaatacaa
    25381 cggtcataat gatgtagccg acaaagaaaa tgaataccgc gttgtcgaac aaaataacta
    25441 tgaaccccat aaagcttgga gtgcgagcaa cttaggccgc ctcgaagatt ataatatggc
    25501 gcgttttaat aagtttgtaa ccgaagttgc gccgattgca ccaactgatg ccggcggcgg
    25561 attggatacc tacaaagata aaaaccgttt ttcctctttt gtcagaatcg gtgcgggcag
    25621 gcaattagtt tatgaaaaag gggtttatca tcaagaagga aatgaaaaag gctacgattt
    25681 gcgcgatctt tcacaagcct atcgttatgc cattgcaggt acgccttata aagatattaa
    25741 tattgaccaa acaatgaata ccgaaggttt gatcggcttt ggtaatcata ataaacagta
    25801 ttcggcagaa gaacttaaac aagcactttc gcaagatgcg ttaacaaatt acggcgtgtt
    25861 gggcgatagc ggctctccgt tatttgcttt tgacaaacaa aaaaatcaat gggtcttttt
    25921 gggaacttac gattattggg caggttacgg aaaaaaatca tggcaagaat ggaatatcta
    25981 taaaaaagaa tttgcagata aaatcaaaca acacgataac gccggcaccg tcaaaggtaa
    26041 tggagaacat cattggaaaa ccacgggtac aaacagccat atcggttcga cagcggtaag
    26101 gcttgccaac aatgaaggag atgcgaacaa cggacaaaat gttacctttg aagacaacgg
    26161 cactttggta ttgaatcaaa acatcaacca aggcgcgggc ggcctgtttt tcaaaggcga
    26221 ttacacagtc aaaggtgcaa ataatgacat cacttggtta ggtgcgggga ttgatgttgc
    26281 cgacggcaaa aaagtcgttt ggcaagtcaa aaatccgaat ggcgacagat tggcaaaaat
    26341 cggcaaaggc actttggaaa taaacggcac aggcgttaac caagggcaat taaaggtcgg
    26401 cgacggtacg gttattctga atcaaaaagc cgatgccgac aaaaaagttc aggctttctc
    26461 ccaagtcggc attgtcagcg gacgcggtac attggtatta aatagttcaa atcagattaa
    26521 tcccgataac ctatatttcg gtttccgtgg cggtcgtttg gatgccaatg gcaatgactt
    26581 gacttttgaa cacatccgca acgtggatga aggcgcgcgc attgtcaacc acaacacagg
    26641 ccacgcctcc acaatcacac taacgggtaa atctttaatt accaatccga acagcctttc
    26701 ggtacatagc atacaaaatg actatgatga agatgattat agttattatt accgaccaag
    26761 gcgacccatc ccacaaggca aagatcttta ttacaaaaac tatcgttatt acgccctaaa
    26821 atccggcggc aacgtgaacg caccgatgcc cgagaacggt gtagcagaaa ataacgactg
    26881 ggtctttatg ggatatacgc aagaagaagc caagaaaaac gcgatgaacc acaaaaacaa
    26941 tcagcgtatt agcggtttta gcggtttctt tggcgaagaa aacgaaaaag gacataacgg
    27001 ggcattaaac cttaatttca atggcaaaag cgcgcaaaac cgtttcttgt taacaggcgg
    27061 cgcgaattta aacggaaaaa taagcgtaac tcaaggcaat gtcttgttat caggtcgtcc
    27121 aacaccacac gcaagagatt ttgtgaacaa atcttcagcc cgaaaagacg cacatttctc
    27181 caaaaacaat gaagtcgtat ttgaagacga ctggataaac cgcacattca aagccgcaga
    27241 aattgcggtt aaccaatccg catcattctc ttccggaaga aatgtatccg acatcaccgc
    27301 caacatcacc gcaacagaca acgccaaagt aaatttgggt tacaaaaacg gcgatgaggt
    27361 ttgcgtgcgc tcggactata ccggttacgt tacctgcaac acaggcaact tatccgataa
    27421 ggctttaaac agctttgatg cgacacggat taacggcaat gtgaatttga atcagaatgc
    27481 ggcattggtc ttgggcaagg ctgcattatg ggggaaaatt caagggcaag gaaacagccg
    27541 tgtcagccta aaccaacata gcaaatggca tttgaccggc gacagccaag tacacaatct
    27601 gtcattggcg gatagccata ttcatttgaa caacgcttcc gatgcgcaaa gtgcaaataa
    27661 ataccacacg atcaaaatca atcatttatc cggtaacggg cattttcatt atctgacgga
    27721 cttggcgaaa aatcttgggg ataaagtgct tgtgaaggaa tccgcatccg gccattatca
    27781 gctccatgtt caaaataaaa caggcgaacc taatcaggaa gggctggatc tctttgatgc
    27841 atcatccgta caagaccgct cccgcctttt tgtttccttg gcaaatcatt acgtcgattt
    27901 aggcgcattg cgttatacaa tcaaaacaga aaacggtatt acccggttgt acaatcctta
    27961 tgccgggaac cgccgcccag tcaagccggc cccgtctcct gccgcaaaca cggcttctca
    28021 agcacaaaag gcaacacaaa cggacggtgc acaaattgcc aagcctcaaa atatcgtcgt
    28081 cgcaccgcct agcccgcagg caaatcaagc cgaagaagcc ctccgccaac aagcaaaagc
    28141 ggagcaagtg aagcgtcagc aagcagcaga agcagaaaaa gttgcacgtc aaaaagacga
    28201 agaggcaaaa cgcaaagcag ccgaaattgc tcgtcagcag gaagaagcac gaaaagctgc
    28261 agagttagcc gccaaacaaa aggcggaagc agagcgtaaa gccagagagt tggcaagaca
    28321 aaaagcagaa gaggcaagtc atcaagctaa tgccaaacca aaacgtcgta gacgtcgggc
    28381 tatattacct agacctcctg cgccagtatt ttcattggat gattatgatg caaaagacaa
    28441 tagtgaatca tcaataggta atttagctcg tgtaatacct agaatgggaa gggagttaat
    28501 taatgattat gaagaaatcc ccttggagga gttggaagat gaagcggaag aagaacgtcg
    28561 ccaagcaacg caattccaac ccaaaagtcg taaccgtaga gctatatcat cggaaccatc
    28621 atctgatgaa gatgcatctg aatcggtttc cacatcagac aaacaccctc aagataatac
    28681 ggaacttcat gaaaaagttg agacggcggg tttacaacca agagccgcgc agccgcgaac
    28741 ccaagccgcc gcgcaagccg atgcagtcag caccaatact aactcggctt tatctgacgc
    28801 aatggcaagc acgcaatcta tcttgttgga tacaggtgct tcattaacac ggcacattgc
    28861 acaaaaatca cgcgctgatg ccgaaaaaaa cagtgtttgg atgtcaaaca ccggttatgg
    28921 ccgtgattat gcttccgcac aatatcgccg gtttagttcg aaacgcacgc aaacacaaat
    28981 cggcattgac cgcagcttgt ccgaaaatat gcagataggc ggagtattga cttactctga
    29041 cagtcagcat acttttgatc aggcgggcgg caaaaatact tttgtgcaag ccaaccttta
    29101 tggtaagtat tatttaaatg atgcttggta tgtggccggc gatattggtg cgggcagctt
    29161 gagaagccgg ttacaaacgc agcaaaaagc aaactttaac cgaacaagca tccaaaccgg
    29221 ccttactttg ggcaatacgc tgaaaatcaa tcaattcgag attgtcccca gtgcgggtat
    29281 ccgttacagc cgcctgtcat ctgcagatta caagttgggt gacgacagtg ttaaagtaag
    29341 ttctatggca gtgaaaacac taacggccgg actggatttt gcttatcggt ttaaagtcgg
    29401 caaccttacc gtaaaaccct tgttatctgc agcttacttt gccaattatg gcaaaggcgg
    29461 cgtgaatgtg ggcggtaaat ccttcgccta taaagcagat aatcaacagc aatattcagc
    29521 aggcgccgcg ttactgtacc gtaatgttac attaaacgta aatggcagta ttacaaaagg
    29581 aaaacaattg gaaaaacaaa aatccggaca aattaaaata cagattcgtt tctaaaatac
    29641 taaattcata gcaaaataaa atgccgtctg aactcaagct tcggacggca tttttatcag
    29701 actaacaaag ctacagctca atgcctttga gtttcgccac ggtattgatg tctttgtcgc
    29761 cgcgacccga caggttgacc aaaatcactt ggtctttacc cattttcggc gcgtttttca
    29821 ccgcccaagc aacggcgtgg ctggattcga gcgcggggat gatgccctcg aatcggcaga
    29881 gcaagtcaaa ggcttcgagt gcttcgtcat ctttggcaac ggtgtattcg acgcgcttga
    29941 tgtcgtgcag atggctgtgt tccgggccga tgccggggta atccaagcct gcggaaacag
    30001 agtgcgtccc caagacttgg ccgttttcgt cctgcatcag atagctgcgg aagccgtgca
    30061 gtacgccaat cggtgcgccc gaagtaatcg gcgcggcgtg gtcaggcgta tccacgccca
    30121 aaccgcctgc ttctacgccg accaaacgta cgttttcttc ttcgatatag ggatagaata
    30181 aaccgatggc gttcgagccg ccgccgacgc aggcaacggc gacgtcgggc tgtctgccgg
    30241 tggcttcctg catctgcgct ttggcttcgt tgccgatgac gcattggaaa tcacgcacca
    30301 tttcgggata cggcgcgggg ccggcggcgg tgccgatgat gtagaacgta tcgtccactc
    30361 gggcgaccca ttcgcgcatc gcttcgttca tcgcgtcttt caaagtacgg ctgccactgt
    30421 caacgccgat gacattcgca ccaagcagct tcatacggaa cacattcggc atttggcgtt
    30481 gaatatcgtc cgcacccatg tacacgtcgc aagtcatgcc gaaacgtgcg gcgacggtgg
    30541 cagaagccac gccgtgctga cccgcgccgg tttcggcgat gacgcgtttt ttgcccatac
    30601 ggcgggcaag caatgcctga ccgatggtgt tgttgacctt gtgcgcgccg gtgtggttca
    30661 agtcttcgcg cttcaaccag atttgcgcgc cgcccaaatg ctcggacaac cgcgcggcgt
    30721 ggtaaacggg gctgggcctg ccgacataat gtttcaaatc gcggcggaac tccgcccaaa
    30781 attcaggatc gtttttcgct tcgttgtagg cctgttccag ctctttcaaa gcgggaatca
    30841 gggtttcgga aacataaagc ccgccgtgtt cgccgaaaaa gcccttctcg tcgggcgcgt
    30901 ggtagttctt catcggattc ttccttatgc ttcaggtttt cagacggcat ttccgttttt
    30961 tcaaacatgc cgtctgaacg ggaaattgtc ggcaattata actgcttttc acacaagccc
    31021 gccatttcga aaaacgcccg atacgccgct gcctttttcg ccaaactcaa aggataggcg
    31081 cgcgaagtcg aaggcaggcg cgtcagtgtc aaatcccgtc cggcaaacgg aaacggcacg
    31141 gtttcgcccg ttttcggcat tttgatgccg ccgccctgaa tatcgagcag gatttccgtc
    31201 gccttgccgc ctgtcgtaca aatatggcgg cagtctggta ttttcgccaa caccgccgcc
    31261 aaatcgacgg tttcaactac ctttaaaaac ttgtcggacg cattgccgtg ctcccttgcc
    31321 gccttcaaca cggtcggaca ggacgcaatc ccccgttcgt gcaaaaacgc cttgattttc
    31381 tccgcgtcaa acgctttttc agagtgcgca gcctccccgt gggacggcat ccggaaatgc
    31441 gccgcatcgt taaaaaacac cagcccgtaa acgcgccaca tatcgttttg gaaattcgga
    31501 taatgaaact gcatcgcgcg tttgtcttcc ttgggcggaa acgtccccat catcatgacc
    31561 gcagccttcg gcggcaacac gggatcgaag ggatgggttt cgatttcaag cagattttca
    31621 gacacgttca atcctccaac aggcaaagcg gcagacagcc ggtttgcacc aaaccgccaa
    31681 acacggcaag ccttcaaaca gcattatcac gccctgccgt tttcggttac aatgccgaca
    31741 tcaaacggat actgtaaaca catttatgtt ccaacacaca ggacgacaca taaagcaccg
    31801 ccctatgtgt cgtcctgatt tggaaggggt tacgccccgc ccaaacagag tctgatcctg
    31861 ccgccctaaa gggcggggtt tcaaccgaag aggaaacacg atgaaagaac acaaagcccg
    31921 caagcgtttc gggcagaatt ttttgcagga cacgcggatt atcggcgata ttgtcaacgc
    31981 cgtgcgcccg caggcggatg atgtcgtgat tgaaatcggc ccgggtttgg cggcaattac
    32041 cgaacctttg gcgaaaaagc tgaaccgcct gcacgttgtc gaaatcgacc gcgacatcgt
    32101 atgccgtctg aaaacgctgc cgtttgcgga taaactggtg attcacgaag gcgacgtgtt
    32161 gcagtttgat ttcaacggca tctcaggcaa aaagaaaatc gtcggcaacc tgccgtataa
    32221 catctccacg ccgcttttgt tcaaactggc ggaggtggcg gacgatgtcg ccgatatgca
    32281 ctttatgttg caaaaagaag tggtcgagcg catggttgcc gcgccgaaaa gcaacgacta
    32341 cggtcgcttg ggtgtgatgc tgcaatattt tttcgatatg gagctgctga ttgacgtgcc
    32401 gcccgaatcg ttcgatcccg cgccgaaaat cgattccgca gtggtgcgca tgattccggt
    32461 gaaacaccgt atcggcaagg cggacgattt cgagcatttc gccaaactgg tgaaactcgc
    32521 cttccgccaa cgccgcaaaa ccatacgcaa caatctgaaa gagcttgccg acgacgatga
    32581 tttgcaggca gtcggcatca gtccgcaaga ccgtgccgaa cacatcgcgc cggagaaata
    32641 tgtggcgttg agcaattatc tggcggacaa ggccgtctga aaagggacgc ggcgatggaa
    32701 agcaacctgc agaaaaccga gcgtttgatc cgcgaaatca accgcctgca cgcacaatat
    32761 tcgcaggatt attttgaaac cggcaaagtg cggaaaatca atttgagcca cacgctcaaa
    32821 aacgttccga ccgaacatat tttgtcttac cgccttaatt tgcacgaagc ggttaacgat
    32881 tatttagcct ttgccgatac gcgcggcata gactttttct accgcgtgaa aaccgcagaa
    32941 agcatacatg ataaaatcaa ccgctatctg gcacgcggca cccaatatcc gaccaacaac
    33001 atcctgaacg atattttcgg cgcgcggctg atttggccgt ctgaaacagt cgccgggatt
    33061 ttagaaaaac ttgacggctg gaaaacagaa tacggcttga aaaactggta tttgcgcgat
    33121 gccggcggct atatcggcat acacatttat ttcaaaaaca gcagcaattt ctactatccg
    33181 tgggaattgc aggtttggga tgaaaaagat gccaaagcca atattgaaaa ccatatggcg
    33241 tacaaacgga atttcgtgcg ttgaattgcc ggcaagccgc cttgaaaccg tttataactg
    33301 tttataagag aataaaccat aaaatacaca accgtattcc tgacttttcc tttaaccgcc
    33361 cgagtgaaca aactatgaaa tacacagccg cactcctgac ttttctttta actgcctgca
    33421 tgaacccgaa cgatgcgttt ttccaaaacc ggcgttatca aatgccggag gcgcaattga
    33481 acggttcaaa cgccgttttc cattacggtt attcccaaaa tcccgatcat gatttattgg
    33541 tcgaccaagt ccgcgtgccg gacgcgtaca gggcttcatt tgcgcgggat tggatagcga
    33601 aggagcaagg ttaccgcaaa aacgactgcg tactggaaaa agcggaaagc gggggtgtga
    33661 cgtactacac ctgcgatgcg gaaaaaggat tcaacggcac acgtttcgtc atctatgtcg
    33721 tgaatatgaa aaacgacaaa ggctacgtca aagtttaccg gggcatgaat aagccgaatc
    33781 aggaagagtt aaccaaattg gttgccgatt tgaataagtt ttacccttaa tcagccagac
    33841 ggattcagac tgcccgaaaa gaatggacac tatgatcaaa ttcaaaaacg tacacaaaca
    33901 tttcaaagac ctgcacgtca tcaacggcgt aaacttggaa atcaaaaaag gcgaagtggt
    33961 cgtcgtctgc gggccttcgg gcagcggcaa atccacactc atccgcaccg tcaaccagct
    34021 tgaaagcatc gaaagcggcg agatttgggt ggacggcgtc aacgttgccg accccaaaac
    34081 cgatttgaac aaaatccgcg aagaagtcgg ctttgtgttt caaagtttca acctctaccc
    34141 gcacctgacc gtgttggaca acatcaccct cgcaccgatg aaggttaagg ggcaaaatgc
    34201 ggaacaggcg gagaaaaagg caatggagct tttggaacgc gtcggactgg cacacaaaaa
    34261 agacgccttc ccttcccaac tttccggcgg ccagcagcaa cgcgtggcga ttgcgcgcgg
    34321 gctggcgatg gagccgcgcg tgatgctgtt tgacgagccg acttccgcac tcgaccccga
    34381 aatggtcggc gaagtgttga aagtgatgaa agacttggcg gaaagcggca tgacgatgat
    34441 gtgcgtaacc cacgaaatgg ggtttgcccg cgaagttgcc gaccgcgtga ttttcgtcga
    34501 taaagggcaa atccttgaag acgaaacgcc ggaagcgttt ttcaccaacc cgaaacacga
    34561 acgcgccaag cagttcctgc aacaagtcat gacccattga tgctatgccg tctgaaaacc
    34621 gaacgttcag acggcatagc ccgacaccgc aagatgattg agaaagcaca ccatgtattt
    34681 tgttgaccgc accgccgccg tcctcaagcc gaccgcgcgc tttttggaat ggctcaaaag
    34741 caccgacgaa aatatgcccg acctgaccat agaacagctt cgcgccgact gttccgtctt
    34801 cctcgttccg cagttcgacg aaccggaagc cgtcgtttcc tacttcgacg aacgctaccg
    34861 gcagattttt gaggcggagc tggcaggttg ggacatcgac aaggacaaat ggccgcaaga
    34921 tatggggctg aaggcgtttt gggaattttt cgacattgaa atccacgata tggttttgga
    34981 tatggaagaa gcggatttga acatcacgcc cgtgttcgac aatatgatgt aacgccatgc
    35041 gcgcctttca gacggcattg aggccgtccc gcatcctgaa catcctgacc gtatcactcc
    35101 actcggcttc cattgccgtc tgcctgactt ggttttacgg ccggatgatg tggttcggtt
    35161 tggcggcttt ggcggcaagt tacgcatact cgctcaggat aacgaatctg aaacaccgcc
    35221 atgccataac cgccattacc atagaccgcg acggacgggc ggaaatcgta tccggcaaag
    35281 acaaaacagc ggcaacactt gcgggcagca gtatggttac gccttatgcc ttattcttac
    35341 aatgggacac aggcggcaaa accgtccgcc aatgcatcat gcccgacatg acggacaaag
    35401 aatcctaccg gcggctgaaa gtctgggtgc tttggcggca gccgaaaaaa accgccgaaa
    35461 ccgatacatc agactgattt cagacggcat ttgaaaacga aacactcatg aaaacactac
    35521 aagactggct ctcgcatttg gaaaccgccc acagcggcgg tttgatcgac atgggtttgg
    35581 agcgcgtaag cgaagtaaaa aagcgcatga acctgacacc gcaatgcccc gtcgtcgtcg
    35641 tcgcgggaac aaacggcaaa ggttcggtct gcgcctacct gacacagatt tacaaacagg
    35701 caggcttcaa aaccggcacg ctgaccagcc cgcatttatt gcattacaac gaacgcatcg
    35761 ccatcaacgc cgaacccgtt tcggacgata cgattattgc ctctttcgag cgcatcgaag
    35821 ccgcgcgcgg cgaaatatcg ctgacttatt ttgaattcaa tgccttggcg gctgtcgaca
    35881 tcttcatacg cgaacaagtg gatgtaatga tattggaagt cggcttgggc ggacgtttgg
    35941 acgcggtcaa cgcgtttgac ggcgattgcg cggtggttac tagtgtggat ttggaccatc
    36001 aggcattttt gggcgatacg gtcgaacagg tcggctttga aaaggcaggc gtgttccgca
    36061 gcggcaaacc cgccatctgc ggtcaaaacc ccgcgcccgc atcattggtc gcacacgccg
    36121 aagccatagg cgcgaaactg ctgatggtac agcgcgattt cgagtttcac gcgatggaaa
    36181 acatccaatg gaactaccgc ttccggccgc agcattcaga cggtcccgca cgcaaccgca
    36241 atgccctgcc cttccccgca ttgcgcggcg cataccagct ttctaacgcc gcctgcgcgt
    36301 tgaccgtatt ggaatgcctc gacgacagac tgccggtgga cattggtgca atcaaacgcg
    36361 ggctgctgct ggttgaaaat cccggacgct tccaagtcct gcccggccgg ccgctgaccg
    36421 tattggatgt cggacacaac ccccacgccg cccgcgccct gcgccgcaac ctgatcaacc
    36481 ttgcctacgc gcaaaagcgc accgccgtgt tcagtatgct gtccgacaaa gacatagacg
    36541 gcgtgtcgga aaccgttaaa gaccagtttg acgagtggta tatcgcgcct ttggacgtgc
    36601 cgcgcggtat gacggcggat gcactgaaag ccaaattgga acaacaccac atcgaaaaca
    36661 tacagacatt cgccgccgtc cgcgacgcgt accgggcggc agcctcaaaa gcgggcgaag
    36721 acgacagaat cgtcgtattc ggctcatttc ataccgtcgc cgacgtaatg tcggtactgt
    36781 gaaaaggaaa cttctatgtc cgaaaacaaa caaaacgaag tcctgaccgg ttacgaacag
    36841 ctgaaacggc gcaaccgccg ccgcctcgta acggcaagct ccctggttgc cgcctcctgc
    36901 atcctgctgg cagccgcact cagttccgat cctgccgaca gcaatcccgc accgcaggcc
    36961 ggcgaaaccg gcgcaacgga aagccaaacg gcaaacacgg cacaaacccc tgccttgaaa
    37021 tccgccgccg aaaacgggga aaccgccgcc gacaaaccgc aggacttggc aggcgaagac
    37081 aagccttctg ccgccgacag cgaaatcagc gagcctgaaa acgtaggcgc gccgctggtg
    37141 ctgattaacg accggctcga agacagcaac atcaaaggtt tggaagaatc cgagaaactg
    37201 caacaggcag aaaccgccaa aaccgaaccg aagcaggcaa aacaacgcgc tgccgaaaaa
    37261 gtgtcggcaa ctgccgacag tacggatacg gtagcggttg aaaaaccgaa acgctctgcc
    37321 gaacccaaac cgcaaaaagc ggaacgcact gccgaagcca agcccaaagc caaagaaacc
    37381 aaaaccgccg aaaaagttgc cgacaaaccg aaaactgctg ccgaaaaaac caaaccggat
    37441 acggcaaaat ccgacagcgc ggtaaaagaa gcgaaaaaag ccgacaaggc tgaaggcaaa
    37501 aagacagccg aaaaagaccg ttcggacggc aaaaaacacg aaacggcgca aaaaaccgac
    37561 aaagcggaca aaaccaaaac cgccgagaag gaaaaatccg gcaaggcggg caaaaaagcc
    37621 gccattcagg caggttatgc cgaaaaagaa cgcgccttga gcctccagcg caaaatgaag
    37681 gcggcgggta tcgattcgac catcaccgaa atcatgaccg acaacggcaa agtttaccgc
    37741 gtcaaatcaa gcaactataa aaacgcaagg gatgccgaac gcgatttgaa caaactgcgc
    37801 gtgcacggca tcgccggcca ggtaacgaat gaatagcctc cccattgccg acctcctcgc
    37861 ctccgccgtc atcgccgcct gcatcgtcat ttccacgatg cgcggcgtga ttgcggaagc
    37921 aggttcgatg gtggcatggg tggtttcctt cttttttgcc aaactctttg ccgcaccctt
    37981 cgccgacctc gcctttgcct cgttccaacc ccgcctgttt gcattggctc tgtcattcat
    38041 ttccctgttc gtcattgcct gtctgatcca gaaaatgctc cgttcgctgc tgaccggcgc
    38101 agtttcggcg gtcggtctgg gctttgccaa ccgcattttg ggcggtgtat tcggtgcatt
    38161 gaaaggcgtt ttgattgtta ccctgctgat catgcttgct tcaaaaaccg acctgcccga
    38221 taccgaagaa tggcaacagt cctataccgt accgtttttc gtatcgcttt ccgaagcggt
    38281 gttaaaccat acggacaacg cacccgaatc cctcgacgac gactaatccg catacgtggc
    38341 gtttcagaca gcatacctga tttatgccgt ccgaaacgcc ttttctttcc ttgacacacc
    38401 ccgacagaca aagcgcacct gcctgacttt acacaaatat tgtcaacaat tcgggcaaaa
    38461 agccgccgtt tgctttataa ttccgtttca cccttaaacc tcgaatcgga gaagacgata
    38521 tgtgcggcgt attaggtttg gtcagtcatg aacccgtgaa ccagcttctg tacgacggtt
    38581 tgcagatgtt gcagcacagg gggcaggatg cggcgggcat tgcaacggcg gaaggcggta
    38641 ccttccatat gcacaaaggc aaagggatgg tgagcgaagt gttccgcacg cgcaatatgc
    38701 gcgatttgac cggcaacgcc ggcatcgccc acgtccgtta tcccacggcg ggcaacgccg
    38761 gcagcagcgc ggaggcgcag cctttctacg tcagctcgcc tttcggcatc gttttggcgc
    38821 acaacggcaa cctcaccaac actgccgaac tgtatgaaaa cgtgtgtaac aaacacctgc
    38881 gccacatcaa caccggctcc gattccgaag ttttgctcaa cgtattcgcg cacgaattgc
    38941 gccgcgaagt ctctaaaaac gccgacccgc accggctcaa tgccgacaat attttcaacg
    39001 ccgttgccga agtccaccgc ctggtgcgcg gtgcatacgg cgtggttgcc atgattgcgg
    39061 gctacggtat gctcgccttc cgcgaccctt acggcatccg cccgctggca ttgggttcgc
    39121 aaaccgacag cgaaggcagg aaatcctatg ccgtcgcctc cgaatccgtc gccttcaatg
    39181 cgcttgccta cgatttggaa cgcgatatcc ggccgggcga agcggtattt gtcggcttta
    39241 acggcacaat catcgcccga caatgcagcg accgcgccaa actcagcccc tgcctttttg
    39301 aatatgttta ttttgcccgc cccgactccg tgattgacgg cgtgtccgtt taccagtcgc
    39361 gcttggatat gggcgtgtcc ttggcggaaa aaatcaaacg cgagctgccc gtggacggca
    39421 tcgacgtcgt gatgcccatt cccgacacca gccgccccag tgcaatggaa cttgccgtcc
    39481 acctcaaaaa gccttaccgc gagggtttga ttaaaaaccg ctatatcggc cgcaccttta
    39541 tcatgcccgg tcagtcgacg cgcaaaaaat ccgtgcgcca aaaactcagc ccgatggaaa
    39601 ccgaatttgc aggcaaaagc gtgttgctgg tggacgactc catcgtgcgc gggacgacca
    39661 gccgcgaaat cgtcgaaatg gtacgcgcgg cgggcgcgcg caaagtctat atcgcctccg
    39721 ccgcgcccga agtgcgctat cccaatgtgt acggcatcga tatgcccacg cgcgaagagt
    39781 tgattgccaa cgggcgcagc gcggcggaaa tcgccgccga aatcggcgcg gacggcatcg
    39841 tgtttcaaga cttgggcgat ttggaagccg tcgtcaaagc actcaacccg aaaatcgaat
    39901 ccttcgattc gtcctgtttc aacggcattt atcggaccgg cgacatcgat gatgcctacc
    39961 tcgaccgcct gtctgccgag aaatccggct gcgccggtct gaaaatccac ccgagcagga
    40021 tggaacacag catcagcatc agcgatgcgg gcgacgaaga ataaggcttc ccaaaccgcg
    40081 atgccgtctg aaatccgaac gttgggtttt cagacggcat ttcccatatc ggcatcacat
    40141 ttcccgtttc atccgcacac aatatgagta ccgaaaccaa aaactacatt acgcccgccg
    40201 gatggcaggc attgaaagac gagctttatc aactggtcaa taaagaacgc cccgaaatcg
    40261 tccaaatcgt caactgggcg gcaggcaacg gcgaccgcag cgaaaacggc gactatcttt
    40321 acggcaaacg ccgtatgcgc gaaatcgacc gccgcatccg tttcctgacc aaacgtttgg
    40381 aggctgccgt cgtcgtcgat cccgaattgc gcgaagcgac cgaccaagta tttttcggcg
    40441 cgaccgtcgg tctgttgcgc ggcgacgggc gcgagcaaac cgtcaaaatc gtcggcgtcg
    40501 atgaaatcga taccgcgcaa aacaaaattt cttggatttc gccgttggcg cgttgcctga
    40561 ttaaggcgcg cgaaggcgac gaagtggttt tgaacacgcc ggaaggccgt gaggaaattg
    40621 aaatcctttc tgtcgaatac atcaaaatcg actgatcggg aatgccgtct gaaacggcac
    40681 aagcgcaccg tttcccaaat aaaggaatac cgaatgagga aaatccgcac caaaatctgc
    40741 ggcatcacca caccggaaga cgcactgtat gccgcccacg ccggcgcaga cgcattggga
    40801 ctggtttttt acccccaaag cccccgcgct atcgacatca ttaaagcaca aaaaatcgcc
    40861 gccgcactgc cgccgtttgt cagcgttgtc gcccttttcg tcaacgaaag cgcgcaaaac
    40921 atccgccgca tccttgccga agtgccgata cacatcatcc aattccacgg cgacgaagac
    40981 gatgcattct gccggcagtt cgaccgcccc tatattaaag ccattcgtgt tcagacggca
    41041 tcagacatcc gaaacgccgc cacgcgcttc cccaacgctc aggcactgct gttcgatgcc
    41101 tatcaccctt cggaatacgg cggcaccgga caccgcttcg actggacgct gttggcggaa
    41161 tattcgggca agccgtgggt gcttgccggc gggctgaccc ctgaaaacgt cggcgaagcc
    41221 gtccgcatca ccggagcgga agcggtcgac gtatccggcg gcgtggaagc gtctaaaggc
    41281 aaaaaagacc ccgccaaagt cgccgccttt atcgcaaccg ccaaccgcct atcccgttaa
    41341 agcaacaaaa attgccgccg gaatgactta tagtggatta acaaaaacca gtacggcgtt
    41401 gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
    41461 ttaatccact ataatctcaa aaatttatgc cattaaatca ataatttctg atgaattttg
    41521 aaaacttaat cccgtcattc ccgcgcaggc gggaacccgg ttcattgagc ttcagctatt
    41581 tagaataaat tttgaaactc taatcgcgtc attcccacga aagtgggaat ccggtttttt
    41641 gagtttcagt catttccgat aaattgcctt agcattgaat gtccggattc ccgcctgcgc
    41701 gggaatgacg gcggaaatct tgtttatatt gaatcaaaaa aacctgcacc ttaatcagtt
    41761 ggcggtttag tccgactttt ggggtgcaga tcaagcttcc agacggcatt tcctttaaaa
    41821 cttcatttcg agcgcgaggc tgaagttcct gcccggtgcg gcataccttc catagttgct
    41881 gtcgccgccg tgccggtttg ccgtgctttc cgcagtctgg cgcaaggatt cccaagtaac
    41941 gtagcggtag ttgccgatat tgtagatagc cgccctcaag gtcagccgtt ttttcagatt
    42001 cagataagcg gaaacatctg ccgttttcca agaagacgta acccttcctg ccgaatatcg
    42061 tttttgatcg cctgccagat aagcaagctc gtcagggttt ttccctttgg aataggtcag
    42121 cataatgttt gcgccccatt tcccctcggg ctggtcgtat ccgaacccca aaacataacg
    42181 cgacggctgt accgcatcca aagcatagct gcggagggac aagtccggcc ggttggatac
    42241 cgatttcggt ttgatacggt tgtacgccaa tgtggtgtac aggccttcgg gcagtttgcc
    42301 gtatacgccg ttccagtcga ttttccccaa gatgttgatg ccttgaagcg acatattttg
    42361 ggcattgtaa taatcgcgta tatcaatctc tgtcaatcgt cctgctgaat ccggcaattt
    42421 ggttttttga tcggcaacgg caatcatatc ggtataacgg ttgcggaaac tgctgatttc
    42481 caaaaagccg aaatcgccct tccactgcaa accgatttcc cggttggctg ccttttccga
    42541 tttcagggcg ggacgctgcc agcctttcgg ataatcgtga taaatgtcta tcccgaaaag
    42601 ttcttggaag gaaggcgttc tgaagccgct ggaggcgcgg taagacacgg aaaaatgccg
    42661 gttcggtttg aacacgatgc cgctgttcca cgaacggtcg gcataccgcc cgctgcggac
    42721 gagttcttcc gacgtggtga agttttgccg gtcatacctg ccgcccaagc tgaaatcaaa
    42781 atatttgccg attgaaaaac ggtcgttcaa agaaatgtgg atattgctgc cgttgatttt
    42841 tcttggcacg catttgcggg aacgcagggt ttcgatgtag ccgcagaccg acccttcgac
    42901 gacttcgggc ttacccaaac ggtacttatc ttgattcttt tcatcgaatc ccgtggattc
    42961 cgaaatcctt gccgcattgt gggaaagctg ttcggggcgg gagatcgctt tggaagcatc
    43021 gtaaccgaag cccaaagtca gatggtgttt cgtccatttg tttttcagcg acttctcaaa
    43081 cgaggcattc aaaacattgt gctgttcgcg gtagtggaaa cggtcgctgc tgtcgtagga
    43141 atacggtttg tccgccgacg cgcggcagga tttgtccacg gcaggataca cggcgcaatt
    43201 cagcttcagc gtgttgttgt cggttgccac gccctgtttg tcaaacgaca acaccgcctt
    43261 atccgcccag ttgtccgagt atttctcatt ttcataacgg tacagcaaac ccatacggcg
    43321 gcggcggtga tgttcgtcaa taaatttggt gcgggaatat ttcaaaccta tgcccttgac
    43381 caaattttta tcgcccttcc actcttctat attcggcaca aaatacaagc cgtcgcggaa
    43441 atcgtcgccg tcgtacaccc cgctcttgtc tctaaacttt tccgcctcgt ccgtaccgta
    43501 atactgtttt tccgtcatat cgcggatgtc gtaacgctgt ttggtatcct caaacacgcc
    43561 gccgacataa tgcctgccgc cgaagcggta gcccagcttg gcaagccaag agccgctgcg
    43621 gtaatccatc ggatcgggca atatcctgcc gccgcccgtg taggcttggg cggacaggtt
    43681 ttcgtggcgc gcctgcgcct cccgcacctg cgcctcttct tcagcactta aaggctgatt
    43741 ttgttcaata cgttctttta cccagcggtt gagctggcgg ttcaaatata tcccgtagcc
    43801 cgccaatttt gccacgggct tgagtgtatt ctcgccctct actaagaaaa atggctctgt
    43861 tgtcttgcgt ttaatatcgt atgtctggcg gaacgcgtcc aaacggtcta tgccgtattc
    43921 caccccgtcc gcaatatcgc cgtgcgggcg cgtttcccgc ccttggcgtt cggttcggat
    43981 tagcagccct tcccaaccgt ctttgctgaa ccccgcgccg agcgacttca taaattggcg
    44041 gtttttactg ccgtaagcgg tttttgcctg tatcccccgg cttttgccgt ctgaaatcag
    44101 gtctgccgcc tctttggtgc ggaaggcgac cgcgccgccg agtgcgccgc tgccgtgatc
    44161 ggacgaaccg gcgcctttgt cgatttccac cgtgctgatg ttttcatatt cgatttcgtt
    44221 gattgcgccg ctgccgccgc gtccgccgta tccgctcaac gatccctgca cggtaaacgc
    44281 ctgtatttgg gcaacgccgt caaccgaaac cgccacacgg tttttatcta cgccgcgtat
    44341 cgagtagccg ccgctcgcgc cgttgccctg ttcgacaacc gccacgcccg gatcgtagcg
    44401 cgtcaggtcg cggataccga gtacctgttc tttgttcaac gtttccgacg ttttgacgat
    44461 tttgcccaaa cctgtcgcct ctttcgatcg ccgtcccact ttggcggcac ggacggtaac
    44521 ctctttcaat gattgacttt gcgccgcatc aagtgccgcc gtttcagggt ttgccgcata
    44581 tgacgggaag gcggcggcaa cagccaaggc agtcagggta agcgggaatg tatgccttct
    44641 gttcattgtg ttgcctcctt gtcatctttt ttcgcaccga ataccgcacc gaatttccgg
    44701 tcgctgtcga gaatgctgcc gcccaattct gccgcctgcg gaccgtaaaa gccgcccgtt
    44761 acaagaagat tgtcggcttt gaagctctgc ggtttggtca accctgcccg gaaaggtcaa
    44821 taccgttatt ccgagtatgc gccaaagcgt ggaaaccgtt accgtcaatc acaccttttt
    44881 caatataaaa agcggggttc gtatcatttt tttctgtcaa cgttccaaaa atcgatttct
    44941 tgccgaaatc aacggtaaat tctgcttttg ccccttgatt cgcatagctg tccttttgaa
    45001 tggacgtagt gccaggctga tcgtctttct tatccggtac gccgatacgc gcttcccaag
    45061 tgccggtata atgcgccgtt ccggtttttg gaatgtcggc ttccgccgtg cggatacctt
    45121 tcaggaaaag gtcgatgtcc ctgcctttag gggcttcctg agcgggcggg atgccgtctg
    45181 aaccgccacc gccgccttct tccggcaatt cttcttcggg ttcttcagct tcttcttcga
    45241 tttcttcagc ttcatcagct tcagcttcat cagcttcttc ttcgacgact tcgtcttcgc
    45301 cgttatcatc ttcggaaact tcgtcttcgc tttcttcggc gatttcttcg tcttcgcttt
    45361 cttcaccgtt attaataccc gaatcctctt cgtcctgcgc cttcggtttg gcggcggggc
    45421 gttcggtttt tatccgtccg agtttcacat aggtcaaaaa gtcgcaacaa gcactgacgg
    45481 tcattttcct gccgtcggca agatcgatgg ttttctcttt gctaaccaaa ggaatttcac
    45541 gcccttcgac aagaagtttg tcgggatgac caaaatcggg catagtggaa acctcaaacg
    45601 ggcggggatt ttcatcagcc gcctcgtcaa cggaaatttt tcgagaatcc aagattttgg
    45661 tgtgttttcc aaacggcata acaggatttg tgcctgatgt ctctgttttt tgtttgcctg
    45721 cgaatacgcc gaatacgccg ttgtcgttac tgataaaccg tccggcaagc tcttctccgt
    45781 tatcgccgaa aaaaccgccc tcaagccgct gatcggcatc ggtatggaaa aacaaatatt
    45841 ctttatcagc gtgtctcgtc ttcacctcgg tgttaacttt ggcactgccg gtaaagcggt
    45901 tgccgtttaa atcggcagtg atgttataaa tggtcagcgg ttttttaggt tcctttggat
    45961 catttctctt ttgcacatac tgatttttaa tcagctcgcc ctttaggatt tttttgtcga
    46021 aatcaaccgt atattcggca ggatgttttt ccctgtcgtc ggcatcctta gccgcataag
    46081 aagttgcacc aatatcgtta ccataaaatg ttatataacc caaatcgtca ctggcaaccg
    46141 ccttacctgt ccgatgacgt ttgacatcgg tcatatattg ccagttgccg gaatattgca
    46201 ccgttcccgc gctcggtaaa gattgggaag gacgttctcc ggaataatat acaaaaccgt
    46261 cataaccaaa acggtgggta aaccgcttac cacctgagtt ctgttcgatt tcatcttttc
    46321 cgtttttagt atatacataa ccggcatcca caaatttata attatatttt ttattttcct
    46381 tttctgacgt cctaatttct acatcggaat cacgtttatg aatttcgctt ttaagttgtt
    46441 gaagtttatt tccttttgaa ccgtataaaa acagaacatc accttcttta aacgacagct
    46501 tttcttctgc ttgtttgcta tcgggaattg ccgtgccatc ttcacgatga aaagcagtat
    46561 tccgcctcaa caaccgcatt gccgcaccga cggcgggccg gttgaccggc gtggtttcta
    46621 ccgaaggctc ggcagggggg ggaagtggga acgtccttag acttgaaagt gacggggtac
    46681 gcggtcggcg ttgattcgac aacaggctgc acgccgaaat tgccgccgca ggatgccagc
    46741 atcaacggca gcaaggcaat accgtaataa ttcagtttac gcatccctat cccccctatt
    46801 taattaataa taattatcat tatattaata tgcacagata atatcaaccc gtttttaaca
    46861 aagatattcc cgattgaaac aaaatgccgt ccgaaacctg ttcagacggc atttgcgtaa
    46921 agttcagcaa gaaaaactac aaacccagct cgcgcaggaa gcgaatgtcg tctgcccaac
    46981 cggatttgac tttgacccag accttcaaaa atactttgtt atcaaacagt ttttccatat
    47041 ccagccgcgc ttcggtggaa atttttttca aacgctcccc gcctttaccg atcaaaatcg
    47101 ccttttggct ttctttgtcg accaaaacgg cgatgtagat gcggttcaaa ccgtctccct
    47161 cttcaaactg ctccacttcg acgttcatcg cataaggcag ctcctcgccc aaatagcgga
    47221 agagtttttc acgcacgatt tccatcgcca aaaaacgcgc cgatttgtcc gtaaccatgt
    47281 cttcgggata catcggtacg ctttcgggca gatacggctt gagcagctcc aacaggttgg
    47341 caatccgcaa accgtgtttc gcactgaccg cctccgccgc cgcaaattca aattcggcgc
    47401 gcacttgggc aacaaacgcc tccagcgcgt aacggtcttt cgccttgtcc ttgtcgattt
    47461 tgttgatcac taaaatgacc ggcgtgtgct tgggcagttg tttcaacacg acgcggtcgg
    47521 catcggtaag gcgcatcgcc tccacgacga aaaccaccac atccacaccg ccgagcgcct
    47581 cggtaacatt ttgattcagc ctgtcgttga gcgcgttgcg gtggtcggtt tgaaagcccg
    47641 gcgtatcgac aaacacgaac tgcgcggtat cgtcggtata aatccccgtt acgcggttgc
    47701 gcgtcgtctg cgcctttttg ctggtaatac tgattttctg accgatgaga tggttcatca
    47761 gcgttgattt gcccacgttc ggacgaccga caatcgccac gaagccgcaa cggtatccgc
    47821 cggcggcgcg ttcccctgca aggaaggttt caatatccat attcgctttc atatccacat
    47881 ttcagacggc atattcacgc gccgcctcat ttctttttct tcttcagcgg cagcttctct
    47941 tccagccatt tcaaagcctc tttcgccgcc tcctgttccg ccgccttgcg gctcgtccct
    48001 ttggcacggc acacgaaacc cagttcgccc aaatcgcagg aaatgacaaa catactgtcg
    48061 ttggcatggc cgatttgctc ttcgatgcgg tatttcggca aggcgaaacg gcgcgcctgc
    48121 aacgcctcct gcaaagcagt tttgccgtct tttgcctgat tttggaaatc ggcgcgccgg
    48181 acgcgttctg caaacaaatg gcgcaccacc ttttccgccg tgttgaaatc ggcatcgaag
    48241 ctgacggcgg caaacatcgc ctccatcgcg tccgccagta tcgaaggccg tctgaagccg
    48301 ctgctcttca actcgcccgc ccccaaatac agaccgtcgc cgacattcat ttccgccgcc
    48361 atttccgcca gcacgccctc attgaccaga ctcgccctca accgcgacaa ctcgccctcg
    48421 gtcaacttcg gaaacgcgtc aaacagcatc cgcgccaccg tataattcaa aatcgaatcg
    48481 ccgacaaact cgaaccgttc gttgtgcttc gcatgatggc tcctgtgggt caaagcccgc
    48541 cgcaaaagcg acatatcgcg gaacgcgtag cctaactttt tctgtatcgc agtatgtgcc
    48601 tgccgtttca aaacatcgtc tttcattacc gtcttccgca aaaaacagcc cgaatgcgtt
    48661 tccatactcc aaaaatacag aaacacctta aggctgcaaa cggcgcacat tgtaccacca
    48721 aaccgccgcc gtctgccgac aacgcgggaa tcgtggtaaa atccgcaagt tcccccccaa
    48781 cgtattcccg caatgaacgc atttgcacaa gcactttcct cggcactcga ccgctgcatc
    48841 gccaccaaca ccgtcgccaa acaaaaccgg cccgtcggct tcctttaccg cgaagccccc
    48901 gtttttgaaa acgacagcgg ctggcgtttc ttcagcggcg acgaaaccga cgaatacacc
    48961 gacgaccccg acaacttcag catcgtcagc ctcgccgaca tcaccaaaac caaccctgaa
    49021 accgccgcac tgctttccca acccgaaggc agcgcgtggg agcttgccga agacggcaca
    49081 ttccaaaccg ttgccgactg gcagccgcaa gactgaaacc ttcagacggc attccgaaac
    49141 cccgaatacc aaaggacacc caatgaacac catcgccccc aacctcgacg gcaaacacct
    49201 ccgcatcggc atcgtacagg cacgcttcac caacgaaatc ggcagccaaa tgctcaaagt
    49261 ctgctgccgc accctccaag aattgggcgt agcagacgaa aacatcaccg tcgccaccgt
    49321 acccggcgcg cttgaaatcc ccatcgcgct gatgaacttt gcctcttccg aaaaatttga
    49381 cgcactgatt gccatcggcg tcgtcatccg tggcgaaacc taccatttcg agctggttgc
    49441 caacgaatcc ggcgcaggga tcggccgcgt cgcactcgac tacaacatcc cgattgccaa
    49501 cgccgtcctg accaccgaaa acgacgcgca ggcaattgaa cggattggag aaaaagcctc
    49561 ggatgccgcc aaagtcgccg tagaatgcgc caacctcgtc aaccttctgc tcgaagaaca
    49621 gtttgaagac gaagaataac aaacagggcg tacaccgatg cgccctttcc gtttcagacg
    49681 gcataccgtg ccgcccaacc gtttcaagga aaaatcatga aaacagcccg ccgccgttcc
    49741 cgcgagcttg ccgtacaagc cgtttaccaa tcccttatca accgcaccgc cgcgcccgaa
    49801 attgctaaaa acatccgcga aatgtccgac tttgccaaag cggacgaaga attgttcaac
    49861 aaactcttct tcggcacaca aaccaacgca gcggactaca tccaaaaaat ccgcccgctg
    49921 ctcgacaggg acgaaaaaga cctcaacccc atcgaacgcg ccgttttgct gaccgcctgc
    49981 cacgagcttt ccgctatgcc cgaaacgccc taccccgtca ttatcaacga agccatcgaa
    50041 gttaccaaaa ccttcggcgg cacggacggg cacaaattcg tcaacggcat cctcgacaaa
    50101 ctcgccgccc aaatccgccc agacgagccc aaacgccgtt gatactcgga aaatccgaat
    50161 cggaaaatac acaaatgccg tctgaaaacc tttcagacgg cattttgctt gaccgttcaa
    50221 tcaatactgc accgtccagc cgatttcgcc gcccgcgcgc atcgggacaa gttcgccgtc
    50281 gccgtaaggg acgcttgcgg gaacggtttg gctttgtttg acaagggtaa tcgtgtcggc
    50341 gttttccgga atgccgtaga accttgcgcc gtttttcgag gcgaaggctt cgagtttgtc
    50401 caacgcgcct gctttttcaa acacttcggc gtaaagttcg acggcggtca tcgcgctgaa
    50461 cataccggcg cagccgcagg cgttttcttt ggcggacttg gcgtgcggcg cggagtcggt
    50521 gccgaggaag aatttgtgcg ccttctcgcc ggtaacggcg gcgaccaatg cctgacggtg
    50581 ggtttcgcgc ttgaggacgg gcaggcagaa atgatggggg cgcacgccgc cgaccaagag
    50641 gtcgttgcgg ttgagcagga ggtgttgcgg ggtcacggag gcggcaacgt tgtcgcccgc
    50701 ttccaaaacg aggcgggcgg cttcggcggt ggtgatgtgt tcgaacacga ctttgagatt
    50761 cggcacttgc gccaaaacgg gtttcatcac gcgcccgata aaggcggctt cgcggtcgaa
    50821 aatgtcgatt tcggggtcgg ttacttcgcc gtgaacgagg aacaggatgc cctgttttgc
    50881 catttcttcc aacacgggga tgagcttgaa caggtcggtt acgccggaat cggaattggt
    50941 ggtcgcgcct gcggggtaga gtttgaaggc gacgatgccg gcggctttgg cttcgcgcac
    51001 gagttcgggc gtggcttggt cggtcaggta aagcgtcatc agcggctcaa acgcgctgcc
    51061 ttcgggcaac gccgccataa tgcgcgcttt gtaggcaagc gcgtcggcta cactgacgac
    51121 tggcggtttg aggttgggca tgatgacggc gcgccccatc tggcgggcgg tataaggggc
    51181 aacggctttg agcgcgtcat cgtcgcgcag gtgcaggtgc atatcgtcgg gtcggatgat
    51241 ggtcagggtt tgcatggttg ttccttttta gatttttggt ttccagacgg catgccgact
    51301 ttatgccgtc tgaagtcagg ggagaattag gggtttcccg ttatgggaac ggtgcgttag
    51361 gttcaatact gcctgtacgg gtaaatccgg ggaatcggcg gtttgtgcgg tcaatacggt
    51421 ttcttcaggg ctgcccgatt cccgccaggc atagctgatt tgcctggttt caaacggtgc
    51481 ctgcaccgac tgttccgccc tcaattctat attcaattaa aaacaaaata gtacaacact
    51541 cgacgttgaa ggtttaacca tggcatactc tgcggactta agaaacaaag ctttaaacca
    51601 tagcggatta acaaaaatca ggacaaggcg gcgggccgca ggcagtacga atggtacgg
//
LOCUS       DAXFWM010000006        47247 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000006 DAXFWM010000000
VERSION     DAXFWM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 47247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47247
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.006"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            239..2500
                     /gene="nuoG"
                     /locus_tag="ACFI8B_000308"
     CDS             239..2500
                     /gene="nuoG"
                     /locus_tag="ACFI8B_000308"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC3833614.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASNRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            2503..3579
                     /gene="nuoH"
                     /locus_tag="ACFI8B_000309"
     CDS             2503..3579
                     /gene="nuoH"
                     /locus_tag="ACFI8B_000309"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC3833615.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSVFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            3660..4139
                     /gene="nuoI"
                     /locus_tag="ACFI8B_000310"
     CDS             3660..4139
                     /gene="nuoI"
                     /locus_tag="ACFI8B_000310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC3833616.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            4151..4822
                     /locus_tag="ACFI8B_000311"
     CDS             4151..4822
                     /locus_tag="ACFI8B_000311"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC3833617.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            4819..5124
                     /gene="nuoK"
                     /locus_tag="ACFI8B_000312"
     CDS             4819..5124
                     /gene="nuoK"
                     /locus_tag="ACFI8B_000312"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC3833618.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            5127..7151
                     /gene="nuoL"
                     /locus_tag="ACFI8B_000313"
     CDS             5127..7151
                     /gene="nuoL"
                     /locus_tag="ACFI8B_000313"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC3833619.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRTKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            7294..7797
                     /locus_tag="ACFI8B_000314"
     CDS             7294..7797
                     /locus_tag="ACFI8B_000314"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833620.1"
                     /translation="MDKRLFDGTVRFEHVTGVGTMQVDFQPNQRVYTMIGENGVGKTK
                     FLECLFTVLLFTYRMANINIGFSWIDLQRLPLKIVELPNGKNEDFRRFSERSNQGFNI
                     NEDVLFSGYQHRLPFVYLPAQSRGWVKKQTSKLSRWEVLKGVQANIWGICFLFFNLAI
                     VHSRLNP"
     gene            7800..8462
                     /locus_tag="ACFI8B_000315"
     CDS             7800..8462
                     /locus_tag="ACFI8B_000315"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC3833621.1"
                     /translation="MENDLEEWIIQRARSANPYQANEDNRKIEIDTLLFLLNQFDGRI
                     APDFLEIKGDDRIFIKLDNQKRELSGLSSGFTSILKIMQSIVAGYSYFTNEQQIANVR
                     GVVLIDEIESHLHNEWQIKIVPLLKKLFPNTTFFITTHSSLVISQLEQGGAYRLVRSK
                     EDGVVYGKMIDYPSNASFVDLLNEAFGVDLNKKKIERAQERNQEQAKKPYWNWYVRNW
                     AD"
     gene            8473..8697
                     /locus_tag="ACFI8B_000316"
     CDS             8473..8697
                     /locus_tag="ACFI8B_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC3833622.1"
                     /translation="MTVYLLDTNYLVYLADDDSDEEKRKAVLSDMAEKLQQDDNRFVI
                     TPLIRYEVLRGVDWGKSEKLSRLTGVLAQF"
     gene            8728..8934
                     /locus_tag="ACFI8B_000317"
     CDS             8728..8934
                     /locus_tag="ACFI8B_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833623.1"
                     /translation="MASNLYRFDKFEAERDNTPKNLEKRKFDMFHYATASVNNLEILS
                     HDTDVNKIKDLHERMRLEDSAELA"
     gene            9044..10540
                     /locus_tag="ACFI8B_000318"
     CDS             9044..10540
                     /locus_tag="ACFI8B_000318"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC3833624.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            10550..11995
                     /gene="nuoN"
                     /locus_tag="ACFI8B_000319"
     CDS             10550..11995
                     /gene="nuoN"
                     /locus_tag="ACFI8B_000319"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC3833625.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            12107..12406
                     /locus_tag="ACFI8B_000320"
     CDS             12107..12406
                     /locus_tag="ACFI8B_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC3833626.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(12523..13419)
                     /locus_tag="ACFI8B_000321"
     CDS             complement(12523..13419)
                     /locus_tag="ACFI8B_000321"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC3833627.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(13403..13627)
                     /locus_tag="ACFI8B_000322"
     CDS             complement(13403..13627)
                     /locus_tag="ACFI8B_000322"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC3833628.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(13740..14663)
                     /gene="rsgA"
                     /locus_tag="ACFI8B_000323"
     CDS             complement(13740..14663)
                     /gene="rsgA"
                     /locus_tag="ACFI8B_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC3833629.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(14668..16524)
                     /locus_tag="ACFI8B_000324"
     CDS             complement(14668..16524)
                     /locus_tag="ACFI8B_000324"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3833630.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRANGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(16550..16687)
                     /locus_tag="ACFI8B_000325"
     CDS             complement(16550..16687)
                     /locus_tag="ACFI8B_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833631.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            complement(16687..17271)
                     /gene="ruvA"
                     /locus_tag="ACFI8B_000326"
     CDS             complement(16687..17271)
                     /gene="ruvA"
                     /locus_tag="ACFI8B_000326"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC3833632.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(17353..17679)
                     /locus_tag="ACFI8B_000327"
     CDS             complement(17353..17679)
                     /locus_tag="ACFI8B_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC3833633.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(17740..18426)
                     /locus_tag="ACFI8B_000328"
     CDS             complement(17740..18426)
                     /locus_tag="ACFI8B_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC3833634.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(18554..19018)
                     /gene="trmL"
                     /locus_tag="ACFI8B_000329"
     CDS             complement(18554..19018)
                     /gene="trmL"
                     /locus_tag="ACFI8B_000329"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC3833635.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(19083..19808)
                     /locus_tag="ACFI8B_000330"
     CDS             complement(19083..19808)
                     /locus_tag="ACFI8B_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC3833636.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCNTDLASFR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     AQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            19807..20556
                     /gene="bioH"
                     /locus_tag="ACFI8B_000331"
     CDS             19807..20556
                     /gene="bioH"
                     /locus_tag="ACFI8B_000331"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC3833637.1"
                     /translation="MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEVFAALYRDFVEGGLT"
     gene            20553..21341
                     /locus_tag="ACFI8B_000332"
     CDS             20553..21341
                     /locus_tag="ACFI8B_000332"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3833638.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPLAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(21422..21529)
                     /locus_tag="ACFI8B_000333"
                     /pseudo
     CDS             complement(21422..21529)
                     /locus_tag="ACFI8B_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            21651..23024
                     /locus_tag="ACFI8B_000334"
     CDS             21651..23024
                     /locus_tag="ACFI8B_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC3833639.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     LWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            complement(23061..25364)
                     /gene="recQ"
                     /locus_tag="ACFI8B_000335"
     CDS             complement(23061..25364)
                     /gene="recQ"
                     /locus_tag="ACFI8B_000335"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC3833640.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(25427..26209)
                     /gene="trpC"
                     /locus_tag="ACFI8B_000336"
     CDS             complement(25427..26209)
                     /gene="trpC"
                     /locus_tag="ACFI8B_000336"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC3833641.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(26260..27216)
                     /locus_tag="ACFI8B_000337"
     CDS             complement(26260..27216)
                     /locus_tag="ACFI8B_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC3833642.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEVSEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(27349..28887)
                     /gene="murJ"
                     /locus_tag="ACFI8B_000338"
     CDS             complement(27349..28887)
                     /gene="murJ"
                     /locus_tag="ACFI8B_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC3833643.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            29325..30023
                     /locus_tag="ACFI8B_000339"
     CDS             29325..30023
                     /locus_tag="ACFI8B_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC3833644.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(30080..31084)
                     /gene="amgK"
                     /locus_tag="ACFI8B_000340"
     CDS             complement(30080..31084)
                     /gene="amgK"
                     /locus_tag="ACFI8B_000340"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC3833645.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            31154..33559
                     /locus_tag="ACFI8B_000341"
     CDS             31154..33559
                     /locus_tag="ACFI8B_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC3833646.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQASDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSQDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            33661..34662
                     /locus_tag="ACFI8B_000342"
     CDS             33661..34662
                     /locus_tag="ACFI8B_000342"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3833647.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(35142..37022)
                     /locus_tag="ACFI8B_000343"
     CDS             complement(35142..37022)
                     /locus_tag="ACFI8B_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC3833648.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGLFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRANGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            37178..37810
                     /locus_tag="ACFI8B_000344"
     CDS             37178..37810
                     /locus_tag="ACFI8B_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC3833649.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            37882..39252
                     /gene="purB"
                     /locus_tag="ACFI8B_000345"
     CDS             37882..39252
                     /gene="purB"
                     /locus_tag="ACFI8B_000345"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC3833650.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            39332..41200
                     /locus_tag="ACFI8B_000346"
                     /pseudo
     CDS             39332..41200
                     /locus_tag="ACFI8B_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(41336..41950)
                     /locus_tag="ACFI8B_000347"
     CDS             complement(41336..41950)
                     /locus_tag="ACFI8B_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC3833651.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGI"
     gene            complement(41986..44136)
                     /gene="dinG"
                     /locus_tag="ACFI8B_000348"
     CDS             complement(41986..44136)
                     /gene="dinG"
                     /locus_tag="ACFI8B_000348"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC3833652.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(44317..45615)
                     /locus_tag="ACFI8B_000349"
     CDS             complement(44317..45615)
                     /locus_tag="ACFI8B_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC3833653.1"
                     /translation="MPSETALLLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(45605..46516)
                     /locus_tag="ACFI8B_000350"
     CDS             complement(45605..46516)
                     /locus_tag="ACFI8B_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC3833654.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            46624..47022
                     /locus_tag="ACFI8B_000351"
     CDS             46624..47022
                     /locus_tag="ACFI8B_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC3833655.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            47071..>47247
                     /locus_tag="ACFI8B_000352"
     CDS             47071..>47247
                     /locus_tag="ACFI8B_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3833656.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEE"
BASE COUNT        10024 a        12471 c        13731 g        11021 t
ORIGIN      
        1 gccgtcattc ccgcacaggc gggaatccat agtggaattt acagaacccg atatttgaaa
       61 agcagttgcc gaaattcaaa aaatggattc ccgcctgtgc gggaatgacg gcaaggtggc
      121 agtaaacgtt ttaaacagta ttgattgtca atgaaactca aaaggccgtc tgaagcccat
      181 ttttcagacg atctccataa aaaattattt atcaaatacc cgtaactagg aacgaaccat
      241 gttacaaatc gaaatcgacg gcaaacaggt atctgtggag cagggcgcga cggtaattga
      301 agccgcgcac aagctcggta cttatattcc gcatttctgt taccacaaaa agctctctat
      361 tgccgccaac tgccgtatgt gtctggtgga tgtggaaaaa gcccccaaac cgctgcctgc
      421 ctgtgccacg ccggtaacgg acggcatgat tgtgcgtacg cattcggcaa aagcccgaga
      481 ggcgcaagaa ggcgtgatgg agttcctgct catcaaccat ccgcttgatt gtccgacctg
      541 cgaccagggc ggcgaatgcc agttgcagga tttggcggtg ggctacggca aaaccaccag
      601 ccgttatacc gaagaaaaac gttccgtcgt cggcaaggac atgggtcctt tggtttccgc
      661 cgaggaaatg agccgctgta tccactgtac ccgctgcgta cgtttcaccg aagaaatcgc
      721 cggtttgcag gaaattgcga tggtgaatcg tggtgaacac tccgaaatca tgccctttat
      781 cggcaaagcg gtggaaaccg agctgtcggg caacgtcatc gatttgtgtc ccgtcggcgc
      841 attgaccagc aaaccgttcc gcttcaacgc gcgtacttgg gaattgaacc gccgcaaatc
      901 cgtttccgca cacgacgctt tgggcagcaa cctgattgta caaaccaaag accataccgt
      961 ccgccgcgtg ttgccgttgg aaaacgaagc catcaacgaa tgctggctgt ccgaccgcga
     1021 ccgttttgcc tacgaaggcc tgtatcacga aagccgtctg aaaaatccga aaatcaaaca
     1081 gggtggcgag tggatggacg tggattggaa aaccgcgttg gaatatgtcc gcagcgcgat
     1141 tgaatgtatc gccaaagacg gcaaccaaaa ccaagtcggt gtttgggcca atccgatgaa
     1201 tacggttgaa gagctgtatc tggccaaaaa atttgccgac ggcttgggtg ttaaaaactt
     1261 tgcaacccgt ttgcgccaac aagacaaacg tctttcagac ggccttaaag gtgcgcaatg
     1321 gttgggacaa agtattgaat ctttggctga caacgatgcc gtattggtag tcggtgcgaa
     1381 cttgcgcaaa gaacagccgc tcctgaccgc ccgcctgcgc cgcgccgcca aagaccgtat
     1441 ggccttgagc gtattggccg gcagtaaaga agaattgttt atgccgcttc tgtctcaaga
     1501 agccgcacat cccgacgagt gggcaggccg tctgaaaaac ctgtctgccg atgcggaaca
     1561 tgccgttacc gccagcctga aaaacgctga aaaagcagcg gtgattttgg gtgcggaagt
     1621 gcaaaaccat cctgattacg ccgccattta tgccgccgcg caagagttgg cggacgcgac
     1681 cggcgcagtg ctgggcattt tgccgcaagc cgccaacagc gtcggtgcgg acgtattggg
     1741 tgtgaactcg ggcgagagcg ttgccgaaat ggcaaacacg ccgaaacagg cagtcttgct
     1801 gctcaacgtc gagcctgaaa tcgatacggt tgacggtgca aaagccgtag ccgcgttgaa
     1861 acaggcgaaa agcgtgatgg cgtttacgcc gtttgtcagc gaaacgctgc tggacgtgtg
     1921 cgacgtattg ctgccgattg cgccgtttac cgaaacatca ggcagcttca tcaatatgga
     1981 aggccgtctg caatctttcc acggcgtggt acaaggcttc ggcgattcac gtccgttgtg
     2041 gaaagtgttg cgcgtattgg gcaacctgtt tgacctgaaa ggttttgaat accacgatac
     2101 cgccgcgatt ctgaaagacg cgctggatgc ggaaagcctg ccgtccaaac tggacaaccg
     2161 cagcacatgg gcaggggagg gcgttcagac ggcctcaaac cgcctcgtcc gtgtcggcgg
     2221 cgtcggtatt tatcatactg attctatcgt gcgccgttcc gcaccgttgc aagaaaccag
     2281 ccatgccgcc gtgcctgccg cgcgtgtaaa tccgaacaca ttggcacgct tgggcctgca
     2341 agacggacaa accgctgtcg ccaaacaaaa cggcgcaagc gtatcggttg ccgtcaaagc
     2401 cgatgccggt ttgcctgaaa acgtggtgca tctgccgctg cataccgaaa atgccgcgct
     2461 gggtgcgttg atgggcacta ttgaactggc gggagcttga ttatgcagga atggttccaa
     2521 aacctctttg ccgcaacgct cggtctgggc gatttgggca tcaccgtagg cttggtggta
     2581 tccgtcatcg tcaaaatcgt gattatcctg attccgctga ttctgaccgt tgcctacctg
     2641 acgtatttcg aacgtaaagt catcggcttt atgcagcttc gcgtcggccc gaacgtaacc
     2701 ggcccgcggg gtctgattca gccgtttgcc gacgtgttca aactcttgtt taaagaggta
     2761 acccgtccga agctgtcaaa caaagcctta ttctacatcg gcccgattat gtcgctcgcc
     2821 ccgtctttcg cggcgtgggc ggtaattccg tttaacgaag aatgggtgct gaccaatatc
     2881 aatatcggcc ttttgtacat cctgatgatt acctcgctgt cggtttacgg cgtgattatc
     2941 gcgggctggg cttccaactc caaatattcg ttcttgggtg caatgcgtgc ttccgcgcaa
     3001 agcatttcct acgagattgc catgagtgcc gcgctggtgt gcgttgtgat ggtatcgggc
     3061 agcatgaact tctccgacat cgttgccgcg caggcaaaag gcatcgcggg cggttctgtg
     3121 ttctcgtgga actggctgcc gctgttccca atcttcatcg tctatctgat ttccgccgtt
     3181 gccgaaacca accgcgcacc gtttgacgtg gcagagggcg agtctgaaat cgttgccgga
     3241 caccacgtcg aatactccgg ctttgcattc gcgctgttct tccttgccga atacattttc
     3301 atgattctga ttgccgcgct gacatcgctg atgttcctcg gcggctggtt gtctccgttc
     3361 ccgcaaagct ggggcattgt cggtacgcct tccgtattct ggatgttcgt gaaaatggcg
     3421 gcggtgctgt actggtatct gtggattcgt gcaaccttcc cacgctaccg ctacgaccaa
     3481 atcatgcgtt tgggctggaa agtgctgatt ccgatcggct tcgcctacat cgtggttttg
     3541 ggcgtgtgga tgatttcacc gctgaatttg tggaaataag tttcagacgg catcttgagg
     3601 ccgtctgaac aaagcgattt tgaataccta acgaaatccc tgttttgagg gaacataata
     3661 tggctaactt agtaaaaacc tttctgcttg gcgaactggt caaaggcatg ggcgtaacgc
     3721 tcaaaaactt ttttgcccgc aaagacacaa tttattttcc cgaagagaaa acgccgcaat
     3781 ccgtgcgttt ccgcggtctg cacgcgcaac gccgttatcc gaacggagag gaacgctgca
     3841 tcgcgtgtaa attgtgcgag gcggtgtgtc cggcaatggc gatcaacatc gaatcggaag
     3901 aacgcgaaga cggcacccgc cgcaccaagc gttacgacat cgacctgacc aagtgcatct
     3961 tctgcggttt ctgcgaagag gcctgcccga ccgatgcgat tgtggaaacc catatttttg
     4021 aataccacgg cgagaaaaaa ggcgacttgc atatgaccaa gcccatcctc ttggcaatcg
     4081 gcgacaaata cgaagccgaa atcgccaaac gcaaagccgc tgacgcgccg taccgctaag
     4141 gagcagacga atgacttttt ccgtgattct gttctatatc cttgccgcca tcgttttgta
     4201 cggtgcggtt cgtaccgtta ccgccaaaaa ccctgtccat gccgctttgc atctggtgct
     4261 gaccttctgc gtgagcgcga tgatttggat gctgatgcag gcggagtttt tgggcgtgac
     4321 gctggtggtg gtttacgtcg gcgccgtaat ggtgttgttc ctgttcgtcg tgatgatgct
     4381 gaatatcgac atcgaggaaa tgcgcgccgg tttctggcgg cacgcgcctg ttgccggtgt
     4441 ggtcggtacg ttgttggcgg ttgccctgat cctgattctg gtcaacccga aaaccgacct
     4501 ggccgcattt ggtctgatga aagacattcc ggccgattac aacaatatcc gcgatttggg
     4561 cagccgtatt tataccgact acctgttgcc gtttgaattg gcggcggtat tgctgctgtt
     4621 gggcatggtg gccgcgattg cattggttca ccgcaaaacc accaatccga aacgtatgga
     4681 tccagccgac caagttaaag tacgcgccga tcaaggccgt atgcgtctgg tgaaaatgga
     4741 agcggtcaaa ccgcaagtcg aatctgccga agaaagcgaa gtttcagacg gcctcaagac
     4801 ggaaggggag ggcaaagcat gattactttg acgcattatc tggtattggg cgctctcctg
     4861 ttcggtatca gcgcgatggg catctttatg aaccgcaaaa acgtgctggt attgctgatg
     4921 tccatcgagc tgatgctttt ggcggtgaac ttcaacttta tcgccttctc gcaacatttg
     4981 ggcgatactg ccggacaaat tttcgtattc ttcgtattga ccgttgccgc tgccgaatct
     5041 gccatcggtt tggcgattat ggtgctggtg taccgcaacc gacaaacgat taatgttgcc
     5101 gatttggacg agttgaaagg gtaatcatga acgatatgac tttatatttg ataattgccc
     5161 ttgttccgtt ggcaggctcg ctgattgcgg gtttgttcgg caacaaaatc gggcgtgccg
     5221 gcgcgcatac ggttacgata ctcggtgtcg ctgtatctgc cgtgctgtcg gcttatgtgc
     5281 tgtggggatt cctcaatggc agccgtacca agtttgacga aaacgtctat acctggctga
     5341 caatgggcgg cttggatttt tccgtcggct tcttggtcga tacgatgacg gcgatgatga
     5401 tggtcgtggt aacgggcgtg tcgttgatgg tgcatatcta taccatcggt tatatgcacg
     5461 atgaaaaagt cggctaccaa cgcttcttca gctatatttc tttgtttaca ttcagcatgt
     5521 tgatgctgat tatgagcaac aacttcatcc agctcttctt cggttgggaa gcggtgggct
     5581 tggtgtcgta tctcttgatc ggtttctatt tcaaacgccc gggcgcgaca tttgccaacc
     5641 tgaaagcctt tttgatcaac cgtgtcggcg acttcggctt tttgctcggt atcggcttgg
     5701 tgcttgccta tttcggcggc agcctgcgct atcaggacgt attcgcctat ctgcccaacg
     5761 tacaaaatgc caccatccaa ctgtttcccg gtgtggaatg gtctttgatt accgtaacct
     5821 gtttgctcct gtttgtcggt gcgatgggta aatcggctca gttcccgctg catgtctggc
     5881 tgcccgattc gatggaaggc ccgacgccga tttccgcatt gattcacgcc gcaacgatgg
     5941 ttacggcggg tctgttcatg gtgtcgcgta tgtcgccgat ttacgaaatg agcagcactg
     6001 cgctgtcggt cattatggtt atcggcgcga ttaccgccct gtttatgggc ttcttgggcg
     6061 tgattcaaaa cgacatcaag cgcgtggttg cgtattccac cctgtcgcaa ttgggctaca
     6121 tgaccgtggc tctgggcgca tccgcctatt ccgtggcgat gttccacgtc atgacccacg
     6181 ccttctttaa agccttgctg ttcttggcgg cgggcagtgc gattatcggt atgcaccacg
     6241 atcaagatat gcgccatatg ggcaacctga aaaaatacat gccgattact tggctgacca
     6301 tgctgatcgg taacttgtcg ctgatcggta cgccgttctt ctcaggcttc tattccaaag
     6361 attcgattat cgaagcagcc aaatacagca ccctgccggg cagcggcttt gcctattttg
     6421 ccgtcctcgc cagcgtgttt gttactgcgt tctacgcgtt ccgccaatac tttatggtgt
     6481 tccacggcga agagaaatgg cgcagcctgc ctgagcatca tgacgatcat catggcgaag
     6541 aacatcacgg cttgggcaaa aacgacaatc cgcacgaaag cccgctggtc gttaccctgc
     6601 ctttgattct gcttgcaatt ccgtccgtca tcatcggcta tgttgccatc gagcctatgc
     6661 tttacggcga tttcttcaaa gacgtgattt tcgtcaacgc cgacgcgcat ccgaccatgc
     6721 acatcatgaa agaagagttc cacggcgcat tggcgatggt gtcgcacagt ctgcattcgc
     6781 ccgtcctcta tctcgctacc gcaggcgtat tgagcgcatg gcttttgtac gtcaaactgc
     6841 cgcacctgcc cgctaaaatc gcgcaggcgt tccgtccggt ttacgttttg tttgaaaaca
     6901 aatactacct cgacgccttg tatttcaacg tcttcgccaa aggcacgcgc gcattgggaa
     6961 acttcttctg gaaagtcggc gataccgcca ttatcgacaa cggcatcgtc aacggctccg
     7021 ccaaactggt cggcgcaatt gccgcgcaag tgcgtaaagt ccaaaccggc tttatctaca
     7081 cctacgccgc cgctatggtg ttcggcgtat tggtattgct cggcatgacc ttctggggat
     7141 tgttccgata aggatgaggc ttcagacggc ctgcttatcg ctggtgtaga ggtcgtctga
     7201 aaagaaggtt tcaggcggca atagattaga gtttcagacg acatctgaca tttcagatag
     7261 tcaggccgtc tgaaaaatag aagggcggat aatatggata aacggctgtt tgacggtacg
     7321 gttcgttttg agcatgtaac cggtgtcggc acgatgcagg tagatttcca gcccaatcag
     7381 cgtgtttata cgatgattgg cgagaatggt gtcggtaaga ccaaattttt ggaatgtttg
     7441 tttacggtat tgttgtttac ttatcgaatg gcgaacataa acataggttt tagttggata
     7501 gatttgcaac gtttaccttt gaaaatagtt gaattgccta atggaaaaaa tgaggatttt
     7561 aggcgctttt cagagcgtag taatcagggc tttaatatta acgaagacgt tttgttctca
     7621 ggatatcaac atcgacttcc ttttgtatat ttgccggcac agagtcgtgg gtgggtaaaa
     7681 aaacaaactt ccaaactcag tcgctgggaa gttctcaagg gcgtgcaagc aaatatctgg
     7741 ggaatttgtt ttctgttttt caatctggcc atagttcatt ctcgcttaaa tccttaaata
     7801 tggaaaacga tttagaagaa tggattatcc agcgcgcccg atcagccaat ccgtatcagg
     7861 caaacgaaga taatcgaaaa attgaaattg atactttact gtttcttctt aatcaatttg
     7921 atggtcgtat tgcacctgat tttttagaaa ttaaaggaga tgatcgtata ttcatcaagt
     7981 tggataatca aaaacgcgaa ttgtcaggat tatccagcgg gtttacgtct attttgaaaa
     8041 ttatgcaatc tattgttgcg ggatacagtt actttaccaa tgagcagcaa attgccaatg
     8101 tgcgcggcgt ggtgctgatt gacgaaatag aaagccattt gcacaacgaa tggcagatca
     8161 aaatcgtgcc gctgctgaaa aaattgttcc cgaacacaac gtttttcatt accacgcatt
     8221 cttctttagt catcagtcag ttagagcagg gaggggccta tcgtttggta cgtagcaagg
     8281 aggacggtgt agtctatggc aagatgattg actatccaag taatgcttct tttgtggatt
     8341 tgctcaacga agcattcggc gtggatttga ataagaagaa gatagaacgc gcccaagaac
     8401 gtaatcaaga acaggcaaaa aagccttatt ggaattggta cgtcaggaat tgggcggatt
     8461 aggagataaa taatgacggt ttatctttta gataccaatt acttggttta tttggcggat
     8521 gatgacagtg acgaagaaaa gcggaaagcc gtattgagcg atatggcgga aaagttgcag
     8581 caggatgaca accgttttgt gattaccccg cttatccgtt atgaagtttt gcgcggtgtg
     8641 gattggggaa aatctgaaaa attgtctcga ttgaccggtg ttttggcaca gttttagtct
     8701 ttggacatta cacagaatgt ttctgatttg gcaagtaatc tgtaccgttt tgataagttt
     8761 gaagccgaac gtgataacac gccgaagaat ttagaaaaac gtaaattcga tatgtttcat
     8821 tatgcgactg ccagtgtgaa taatttagaa atcttatccc acgataccga tgttaataag
     8881 attaaagatt tacatgaaag aatgaggctg gaagattcgg cagaattagc ttgagacaaa
     8941 tagcagaaac ctgaacaaac ctttttcaga cggcctctta aaacgtcgtc tgacaaacca
     9001 acccgtacaa cgatattttt attttaacca caggttaacc actatgtttt ccaactacct
     9061 actcagcttg gcaatatgga tacccatcgc cgcaggcgtg ctggttttgg cgaccggtaa
     9121 agacagccgt gcgccgctgg cgcgtgtgct tgccttcatg ggagcgcttg ccggtttctt
     9181 ggtaacgctg cccctgttta ccggtttcga ccgtttgagc ggcggctatc aatttaccga
     9241 gttccacgag tggattccgc ttctgaaaat caactacgca ttgggcgtgg acggtatttc
     9301 agtgctcttt atcatcttga atgcgtttat tacgctgctg gtggtattgg ccggttggga
     9361 agtcattcag aaacgtccgg cgcagtatat ggcggcattc ctgatgatgt cgggtttgat
     9421 taacggcgcg tttgccgcgc aggatgcgat tctgttttat gtgttcttcg agggtatgct
     9481 gattccgctg tacctgatta tcggtgtatg gggcggcccg cgccgcgtct atgcgtcggt
     9541 caagctgttc ctctatacgc tgacgggttc gctcttgatg ttggttgcca tggtttacct
     9601 gtactatcag acaggcagct tctctattgt cgatttccaa aacatcaaac agattccgtt
     9661 gggcgtacaa cagcttttgt ttgtagcgtt cttcctgtca tttgccgtaa aagtgccgat
     9721 gttccccgta cacacttggc tgccggatgc ccacgttgaa gcgccgaccg gcggttcgat
     9781 ggtgttggcg gcaattacgc tgaaactggg tgcgtatggt ttcttgcgct ttatcctgcc
     9841 gattatgccg gacgcggctc gctattttgc ccccgtaatc atcgtattga gcctgattgc
     9901 cgtaatttat atcggtatgg tggccctggt gcaaaccgat atgaagaaac tggtggcgta
     9961 ttcgtccatc agccatatgg gttttgtaac gcttgggatg tttttgtttg ttgacgggca
    10021 gttggacgac tgggcgttga aaggcgcggt cattcagatg atttcgcacg gcttcgtgtc
    10081 ggcggcgatg tttatgtgca tcggtgtgat gtacgaccgc ctgcacacgc gcaatattgc
    10141 cgattacggc ggcgtggtca atgtgatgcc caagtttgcg gcgtttatga tgttgttcgg
    10201 tatggcgaac gccggcctgc ctgcgacttc cggcttcgtg ggcgagttta tggtggttat
    10261 gggcgcggtc aaagtgaatt tctgggtggg cgcgctggcg gctatgacgt tgatttacgg
    10321 cgcgtcttat accctgtgga tgtacaagcg cgtgattttc ggcgcgattc acaatccgca
    10381 cgttgccgaa atgaaagaca tcaattgccg cgagtttgcg attctggcgg ttttggcggt
    10441 tgctgttttg gggatgggcc tgtatccgaa cgcatttatc gaagtggtgc atcaggcggc
    10501 aaacgatttg attgcccatg tggcgcaaag caagatttga ggtgtgtaaa tgaattggtc
    10561 agatttgaat ttaatgcccg cactgcccga agtcgcgctg ctgtcgctgc tggtgttatt
    10621 gttgccggcg gacttgtggg ccagtgatga caaatgccgt tggacgcatt acggcgcgtt
    10681 ggcaacggtg gcggttacgg cggcggtgca gcttgccgtg tgggaacagg gcagcacgtc
    10741 ttcgttcaac gggatgtata ttgcggacgg tatgtcgcgt ttggcaaaaa tggttttata
    10801 tgccttgacg tttgtcctgt ttgtctatgc caagccctac aaccaagtgc gcggtatttt
    10861 caaaggcgag ttttacaccc tgtcgctgtt tgccctcttg ggcatgagcg tgatggtgag
    10921 cgcggggcat tttttaactg cctatatcgg tttggaactc ttgtcgctcg ccctttacgc
    10981 cctgattgcc ctgcgccgcg attccggttt tgccgccgaa gccgccttga aatattttgt
    11041 tctgggcgcg ctggcatccg gcctgctgct ctacggtatt tctatggttt acggcgcaac
    11101 cggttcgctg gaatttgccg gcgtgctcgc ctcttccttc aatgaagaag ctaacgaatg
    11161 gctgttgaaa ctgggtctgg tgtttatcgt cgtcgccgtt gcgttcaaac tcggcgcggt
    11221 accgttccat atgtggatgc ccgacgtgta tcacggcgcg cccacttctg ttaccgcctt
    11281 ggtcggcact gccccgaaaa tcgccgccgt cgttttcgct ttccgcatcc tcgttaccgg
    11341 gctgggaacc gtgcatcatg actggtctct gatgtttgcc ctgcttgccg ccgcctcgct
    11401 gctggtcggc aaccttgccg ccatcatgca gaccaatatc aaacgtatgc tcgcctattc
    11461 caccgtatcg catacgggtt tcatcctgtt ggcgtttatg gcgggcgcgg tcggctttgc
    11521 ggcgggcctc tattacgcca ttacctacgc gctgatggcg gcggcagggt tcggcgtgtt
    11581 gatggtgttg tcggacgggg acaacgagtg cgaaaacatc agcgatttgg cagggttgaa
    11641 ccaacaccgc gtatggcttg cctttttgat gctgctggtt atgttctcta tggcgggcat
    11701 tccgccgctg atgggttttt acgccaaatt cggcgtgatt atggcgcttt tgaaacaagg
    11761 ctatgtatgg ttgtcggtgt ttgccgtcgt gatgtcgctg gttggcgcgt tctactacct
    11821 gcgcgtggtc aaagtcatgt attttgacga atccggccgc gcccgtcccg ccgccggcgg
    11881 caacaatgcc gcaaaatccc tcttaagcgt caacgcgctg ctgttggtgc tgtggggcat
    11941 tatgccgcaa accgttatcg actggtgcgc caaggcgttg gagaacacgc tgtgagccgc
    12001 cgcaacggca gccgtgtcag aggctgccgt ttttgttaag atatgccgtt ccgcaatgca
    12061 gttcagacgg catcgccgcc gacaatgccc aaacagaaag cccatcatga ccgcatccat
    12121 gtacatcctt ttggtcttgg cactcatctt tgccaacgcc cccttcctca cgaccagact
    12181 gttcggcgtg gccgcgctca agcgcaaaca tttcggacac cacctgatcg agctggcggc
    12241 aggtttcgcg ctgaccgcct ctcttgccta catcctcgaa tcccgtgcgg gagcggtaca
    12301 caatcagggt tgggagtttt acgccaccgt cgtctgcctg tacctcattt tcgccttccc
    12361 gtgtttcgtg cggcggtatt tttggcacac gcgcaacagg gaataaacaa gcaaacaagc
    12421 ataggaatgc cgtctgaaac cctttcaggc ggcatttgtt tcattcaagt gcaggccggc
    12481 atcgctgtgc cggcacgttt cagtcggcga tatacgccgg ttttaatatt tgcgtgcgac
    12541 tgcaaattct gccaactgcc gcaggcgcag ggctttgtcg ccgaagggtt cgagcagcgc
    12601 gactgcttcg gcaaccagtt tgtgtgcgta tgagcgcgcc gcttccaagc ccatcagttt
    12661 cacataagtc ggcttgtcgt tgtcggcatc tttgcccgcc gtcttgccca aagtcgccgt
    12721 gtccgcttca caatccaaca catcgtcgat gacttggaac gccaaaccca gttttgccgc
    12781 gtaagcgtcc aatacggcaa gttccgcgtc ggacagatcg ggacacgccg tcgcccccaa
    12841 taaaaccgcc gcacggatca acgcccccgt tttcaggctg tgcatccgtt ccaaatcggc
    12901 ttgaaccatt tgtttgccga catttgccaa atcgattgcc tgtccgcccg ccatacccgc
    12961 gctgccgccc gcttttgcca acaccgacaa cattgccaac tggcgtgcgg cgggcagttc
    13021 tgtcggacgg ctcaacacgt cgaaggcttg ggtttgtaaa gcgtcgccgg tcagaagggc
    13081 ggtggcttcg ccatatttga tgtggcaggt cggtttgccg cgccgcaggc tgtcgttgtc
    13141 catcgccggc atatcgtcgt gaaccaaaga atagacgtgg atcatttcga ttgccgccat
    13201 tgcctgtccg accgcatccg ccacggcccc gcccaattcc gaagccgcca gcaccagcat
    13261 cgggcgcaga cgcttgccgc cgtccaaagc cgcatagcgc atcgcttcgt gcagcgtgtg
    13321 cgggatttcg tttccagacg gcaaaaagcg ttccagcagc agctctgtct gcgcctgcgc
    13381 cctctgttgc cacgctttca aatcattcgt cggattcaag gttcaactcc ttcgtcccgt
    13441 ccgcatccaa aacctgtaat ttctgttcga cttgggccag cttggtttgg cagtacctga
    13501 ccagctcgtt gccttcctga taggcggcaa gcgcgtcttc caagggcatt tcgccctgca
    13561 tggactgcgt cagcgattca aggcgcgaca aggcctcttc aaacgatttc ggggtgtttt
    13621 tcttcatagt ttcgggtgtt ccggtaaaaa gcggattgta gcatttttgc aaaacggatg
    13681 ccgtctgaaa cccgaatccg gcttcagacg gcattttttc cgccccggtc ggcaaggcgt
    13741 tacccgagca gttcgtcggt gatgccctgc aaaaaggcga ggcgttcggg gcttgccgcc
    13801 ccggtttcgg cggcggcttt gaaggcgcag ccgggttcgg cgcggtgggt gcagttgtgg
    13861 aagcggcatt gcccgacaag gtggcggaaa tcggggaaat agtgcggcaa atcggcggct
    13921 tggaggtggt gtaaaccaaa ttcttgcaaa cccggggagt cgatgagttg ggtttcgccg
    13981 ttcaaatcat aaagccgggc gtgggtggtg gtgtgttttc ccgagtcgag tgcggcggaa
    14041 atgtcgccgg tgcgggcggt ttggctgcct aaaagggcgt tggtcagggt ggatttgccc
    14101 ataccgcttt gtccgagcag gatgttgctg tgcccttgca ggacggggcg caggctgtcg
    14161 gcgttttcca gcacgcgggt ttcgatgacg gggtaaccca gcgtttcgta gaatttgagt
    14221 ttttcgagcc agagggcggt ttcgggcagg tcggctttgt tcaggacgat gacggcgcgg
    14281 atgccggcgg cttcggcggc aagcagggcg cgctgtagca gccgcacgct cggactcggg
    14341 acggcggcgg ttacgatgag gagttgggta acgttggcgg cgatgagttt ggttttccac
    14401 gcgtcttggc ggtagagcag gctttggcgc ggtaaaaaat cttcaatcac aacttgttcg
    14461 gcgttgacgg ggctgatgcg gacgcggtcg ccgcaggcga aatcgacgcg ttttttgcgg
    14521 gtgctggctt cgtaggttgt gccgtcgggc gtgcggacga tgtagcggcg gccgtagccg
    14581 gcggtaattt gggcggtgtc gttcatggtt tctttggggt tgggtgtgga aatgccgtct
    14641 gaaaaggggt gttcggacgg catcggttca gtcgtgctgc cactcgacgt gttcgctgag
    14701 gaagccgccg ctctggtgcg cccagagttt ggcgtaaagc ccgcgttttt cgaggagttc
    14761 ggcgtgtgtg ccttcttcga tgatgcggcc tttgtcgagg acgacgagcc tgtccatcgc
    14821 ggcaatggtg gagaggcggt gggcgatggc gatgacggtt ttgccttcca tcattttgtc
    14881 gaggctttct tggatggcgg cttcaacttc ggaatcgagc gcgctggtgg cttcgtcaag
    14941 cagcaggatg ggtgcgtctt tgagcatcac gcgggcgatg gcgatgcgct ggcgttgccc
    15001 gccggagagt ttcacgccgc gttcgccgac gtgcgcgtcg tagccgctcc gccctttggc
    15061 atcggaaagg tcggggatga agccggcggc ttcggcgcgt tcggcggcgg aaaccatttc
    15121 ggcatcggtc gcgtcggggc ggccgtaaat aatgttgtcg cgcacggaac ggtgcagcag
    15181 cgaggtatct tgtgtgacca aaccgatttg ggcgcgtaaa gattcttggg taacgccgct
    15241 tatgtcctgc ccgtcgatcg aaaccgtgcc gctttgcggt tcgtagaaac gcaaaagcag
    15301 gttgacgatg gtggatttgc ccgcgccgct gcgtccgatc aagccgactt tttcgccggg
    15361 tttgatattc aggttaaagc cgttgagcag cggtttgccg gcttcgtagc aaaaatcgac
    15421 gtgttcgaac ttgattgcgc cttgcggcac gttcagcggc agggcttggg gcttgtcgag
    15481 gatggtgtgc ggtttggaca gggtcgccat gccgtcgttg acgatgccga tgttttcaaa
    15541 cagccgcgcg gattcccaca taatgtattg cgacagcccg ttggcgcgca acgccatcgc
    15601 ggtggcggtg gcgaccgcac ccacgccgac ctgtccgttg tgccaaagcc agatgcccag
    15661 tgcggcggcg gagagggtca gcgaagtgtt gacgatgaag ctgcacgaat gcagcagagt
    15721 cgccagccgc atttgggcgc gcaccgtaac cataaattct tccatcgact gcttggcata
    15781 gacggcttcg cgcgcgccgt ggaaaaagag tttgacggtg gcgatattgg aataggcatc
    15841 ggtaatgcgg ccggtcatca gcgagcgggc attcgcctgc cgtgcggcgg tttgccccaa
    15901 tctgggaatc agcaggcgca ttaccgaagc gaaaccgatt atccagccga taaagggcag
    15961 cagcagccat gagtcgagcg aggcgagaat cacgcccgaa gtgatgaaat acaccgacac
    16021 ataaacgacc atatcggcaa ccgtcatcac cgcgtcgcgc aacgccagcg cggtctgcat
    16081 gactttggcg gacacgcggc cggcaaattc gtcctgataa aaaccgaggc tctggttcag
    16141 catcaggcgg tggaagttcc agcgcaggcg catagggaac acgccctgaa gggtttgcag
    16201 gcgcacgttg gacgcggcaa acgcccacac gaccgaaaac accatcatcg ccgccattgc
    16261 cgccagttcc caaccttttt cggcaaacag ttcggcgggc gcgtatttgc cgagccactc
    16321 tacgattttg cccataaatt gaaaaatcag ggcttccata atgccgatgc cggcggtcag
    16381 cgcagctagg gcggctatcc atttccgcac cccgtccata ctgctccaga caaaccggca
    16441 caagcctttt tcgggcgttt tcggcgcggc ttcggggtag gggtcgattc gggactcgaa
    16501 ccaggaaaat attttgttca acattgtttt cgatttcggt aaaacagttt cagacggcat
    16561 caaacacaat gccgtccgaa aggagggaca ataacgccat tttacgggaa aagccgtcgg
    16621 gaagacagcg cggggcggaa acgcagggtt tcgtcagggc aaacgccgcg ccgccttcgg
    16681 acggcattat ttcagcaggt ttttcagggc aaggcgcacg ccttcgccca cgtccgtccc
    16741 cttcggaacg cctttgaccg ccgcttttgc ctcgcgttcg ttgtaaccca gcgcaagcag
    16801 cgtgctgacg atgtcttccg tttcgtcggc ggcgggtgag gcggcaaaca gcccgtccgt
    16861 taccgtatgc gcgaccagct tgccgcgcag ttccaagacc atacgttcgg cggttttttt
    16921 gccgattccc ggagcggagg agaggcgttt gacatcttct tctgcaaccg cccgcgccag
    16981 ctcgtctgcc gtcatcgccg acaaaatgcc caaagccgtt ttcgcgccga tgccgccgac
    17041 cttaatcagt tggcggaagg tcttgcgttc ttccgcagtg gcaaaaccaa ataaaagatg
    17101 tgcgtcttcc cgaatgataa gctgggtaaa cagttgtacg ctttcaccca cgggcggcag
    17161 gttgtagaag gtctgcatcg atacgtcggc ttcataaccg acaccgttga catcgatgac
    17221 gatttgcgga gggttttttt caaccagttt gccggtcagt ctgctgatca tgtgtgccga
    17281 atcctgaagt gtcgggtgca aaatgccgtc tgaaaccggt tcgggctcca gacggcacgg
    17341 attgtatcaa attcagtcgt cgcggcggga ggaaatcacg cggccggtac gggcatcgac
    17401 aacgactttg tattcctgtc cgtttttgac gatttcgaca tcatagtgcg gacggccgtc
    17461 gtcgtgttcg agatcgatgt cggtgatttt gccgccgaca cgcgcccaag ccgctttttc
    17521 ggcttgggcg cggctgatga ttttgtcttg tttgccgtgt tggtgtgcgg cgtgtccgtg
    17581 gtcgtcatcg ccgtgtccgt cgtggtgggc gagtgcgggg gcggaaatgc ccaacagtgc
    17641 ggttgcggcg gcggtcaaga gaaggtgttt gatgttcata tttgcccttg taaatcaggg
    17701 gttgaaaaat ggggatatta ataaggtatc gaataaccgt caaccggagg tcagtaccgc
    17761 ccgaaccata ccgcgtgcct gcgcttcggc ttcggcggcg cgttcctgcg aggcaaacgg
    17821 gcccattttg acaacgtatt cgtaacggcg tttttcaacc gagaggttcg ggcttgatga
    17881 cgaagcggcg aaattttggg cggcttggtt cagataggct tgtgcttcgt gttccgtacc
    17941 gaaagatttc aagtcgataa agatgtcttt gttttcggca accggtgcgg attggcccgg
    18001 gacgatttgt tcgattttga cgtgtgccgt cccttggctg acaaagccca atttttgcgc
    18061 ggcggctttg gatacgtcga tgatgcggtt gccgtggaag gggccgcggt cgttgacgcg
    18121 gacgatgacg cttttgccgt ttttggtgtt ggttacgcgc acatggctgg ggatgggcag
    18181 ggttttgtgg gcggcggtaa aggcgttcat atcgtagcgg tctccgccgg aagttttgcg
    18241 cccgtgaaac ctgccgccgt accacgaggc gttgccggtt tgcgtgaatt cggcgacttt
    18301 gtagctgcgg ttggcggagg cgtgcagttt ttccgccttg accactgcgt cggcggatgc
    18361 cgtctgaagg gagtgtgtgc cgaacgcggc ggtgaggagg aaaagggttt ttcgggttaa
    18421 agtcaaaatg tgttccgttc ttgagttgaa gacggatggg cattatgccc tacggacgcg
    18481 ttccgaaccg ccgcacgctg cggcagcggt tcggaatgtg tccgaacggt tttcagacgg
    18541 catgaacctg cgttcaaacg ccgcctgcgt aaccgtgttg ccgccacgct tcaaagagaa
    18601 tcacggagac ggtgttggaa aggttcatac tccggctgcc gggccgcatc ggcaggcgga
    18661 ttttttgcgc ggcgggcagg ctgtcgagga tgtcggcagg cagcccgcgc gtttccggcc
    18721 cgaacagtaa aacgtcgcct ttttgaaacg cggtttcatc ggggcgcgcc gtgcctttgg
    18781 tggtcagggc gaaaatacgt cggcctgcca gcgacttgag gcagtcgtcg aagttttcgt
    18841 gcaccgtcag gctggcgaat tcgtggtagt cgagcccggc gcgtttcatt ttggcggaat
    18901 ccaatgggaa gccgagcggt ttgacaaggt gcaaatccgc gccggtattg gcgcacaggc
    18961 ggatgatgtt gcccgtgttc ggggggattt ccggctgata taaaacgatg gtaagcataa
    19021 atatcaatca cttataggcg cgtaaccttg ccataaggcg gatggggtgt caaaaaattt
    19081 agttattttt tcattggcgt gcgtgccagc gtccagcagc agattcggtt tgcgcccgat
    19141 tttttcagcg tctttgccaa ttcgtccagc gtcgcgccgg tggtaaagac atcgtcgatt
    19201 aacaaaatat tacagttttc cggtatcggt gtgcggattt caaaggcgtt tttgatgttt
    19261 cgccgccgtt cgccgccttt gagcgtgctt tgcggcgggc ggtggtgtcg gaatacggtg
    19321 tgtcggggca gtatctgcca gccgtagcgt tgcgccaaca acccgacgat gctctcgctt
    19381 tggttgaacc cgcgttgcag cagccgctcc ctgcttagcg gaacgggtag gacgaaatcg
    19441 aaacattcgt ctgaaagccg gtcgggcgga ttctgcatcg tcaggtctgc cagcggctgc
    19501 gccatgccca aatcagccaa gtgcttcagc gcgcgtatca tattgctgac gggcggttcg
    19561 taatgcagcg atgcccacat ccggtcgaat gcgggcggtt ttttctgaca tccgccgcac
    19621 accgcgccgc cttgaacgtg cctgaaacac agggggcagc tgtttgccgc gtcggtgcgg
    19681 aaacttgcca aatcggtatt gcagccggcg cagatgccgt ctgaaacgcc ggacgaaccg
    19741 tggcataata cgcaacgcct gatagtgggc gcgtctgcga tgcgccgcca acaagagaga
    19801 aaattcatgc ctgatgctgc caaaaaagtt tacctgatac acggttgggg ggcgaaccgc
    19861 cacgcgttcg acgatttgat gccgcgcctg cccgcaacgt ggccggtgtc cgccgtcgat
    19921 ttgcccggac acggggacgc gcccttcgcg caacccttcg atattgaggc agctgccgac
    19981 ggcattgccg ctcaaatcga tacgtcggcc gacattctcg gctggtcact cggcggattg
    20041 gtcgcgctgt atctggcggc gcgccatccc gacaaagtcc gttcgctctg cctgacggcg
    20101 agtttcgcac ggctgacggc tgccgaagac tatcccgaag ggcttgccgc gcctgcactg
    20161 ggcaaaatgg tcggcgcgtt ccgaacggat tatgccaaac atatcaaaca gtttctacaa
    20221 ctccagcttc tgcacacgcc tgatgccgcc gaaatcatcg gcagaatcct gcccgatttg
    20281 gcgcgctgcg gcacgcctca agccttacag gaagctttgg acgcggcgga aagggcggat
    20341 gtgcggcatt tgttggacaa gatagatgtt ccggtactgc tggtgttcgg cggcaaagac
    20401 gcgattacac cgccgcgtat gggcgagtat ctgcatcggc atttgaaagg aagcaggctg
    20461 gttgtgatgg aaaaggcggc acacgcgccg tttttgagcc atgcggaagt gtttgccgcg
    20521 ctgtaccgcg actttgtcga agggggtttg acatgaatcc gcaggacaaa tgctggcagg
    20581 ttcaccgcca tcttgccgaa cataccgacc aacgcctgac actcgtccgc aacgcgcccg
    20641 ggcatatcct gcttgccggt gcggatgcgg acatcagccg cagcctgttg gcgaagcgct
    20701 atccgctggc ggtatttgaa gaatacgatt cccgtgcgga ttttttggcg gctgccgcag
    20761 ccgcgcgcaa gggcggtttc tggcaaaaac tgacgggcag gggcgtggtg cagcactgcc
    20821 aatccccgac cgtgccgctg cccgaagcgt gtgccgatat gttgtggtcg aacctcggac
    20881 tgttggcggc ggaacaaatc cttcccgtgc tgcacaactg ggcgcgcgcg ctgaagacgg
    20941 acgggctgct gtttttcacc tgcttcgggc gcgatacctt ggcggaactg aaatcccgtc
    21001 tgaaagaaaa cggcattgaa agccgcagcg tgatgtttcc cgatatgcac gacttgggcg
    21061 atatgctcgc cgaaaacggc ttttacgatc cagttaccga tacggcgaag ctggtgttgg
    21121 actacaaaaa ggcggaaacg ttttgggcgg atatggacac gctgggcgtt tggcgggcgg
    21181 tggcgtggga cgacgaaaac gccgcgcgtt cgtgtgtcgg ggcgatattt gaacgggaag
    21241 gcggtttggg cattacgctg gaaacggtgt acggacacgc cgtgaaaaaa ctggtgctgc
    21301 cgcaggggga gaacgtggtg cgtttttttc cgaagagatg atgtgcagat gacgtctgaa
    21361 gccgtttcca agtttcagac ggcatttgtc tgtgaaaacc gacagaaata aaggaaatgc
    21421 cgatgtatag tgaattaaat ttaaaccggt acggcgttgg ctcgccttgc cgtactatct
    21481 gtactgtccg cggcttcgcc gccttgtcct gatttaaatt taatccacta tgtgccgatg
    21541 ccggagacgt atattgcgtc tataacatca gactgaagca gtacactgcc tgccaggtta
    21601 cccgagttga agaacacggt ggcaaaaaaa cacatgcgac cctgctggct ttggactggc
    21661 ggggcaacaa accgcttggg gcggcggagc tggcggattt gaaaccgctt tacaaagact
    21721 ttatgtattg ggaacgcggt ctgcacatgt ataaggcaag tgccgtcgtg ccgacgggat
    21781 atgtacgggt tgggaatacc gcgccgctgt gcggcgaaga cacgcagcgg tatgcctctt
    21841 tttggggcga cggctacgac gtgtaccgtc agttgagatg gcggcagata cccgaaaaac
    21901 agagaaaggc attcaaaaaa gccgccaaaa gcaaaaatac cgtgatgttt gccggacggg
    21961 aatacggcat atccaaacag aatttgagcg atgtttggga tgattttgaa gacgcgatgg
    22021 aactgaaggc gtttccctgc ctgtcttcgc tgtttctgac caagtggcat aaaaatctat
    22081 atgaatatct ggaggaatat ccgtttatca cccgattgtg tctggaaaac cacggtcaga
    22141 ccgtgctgga tttcagcaat acgcgcataa ccgacctttc tgtcgatatg accggcgtgg
    22201 aatcgcttta tctgaacgaa ggcttggaca gcctgaacct gaaaggcgaa atcaaggaaa
    22261 actgcaaggt ctgtaccgcc ggaaagggcg cgggtctgat tttggaagtg ggcaaaagcg
    22321 ttcccaaagt gcggggtttg gaaaacctga ccgccgtcaa cgtcatgggc attgccgatt
    22381 tcgatatgca aaacctgtcg gaaacgtatc ccaagctcaa aaccatacgg ctgtggggca
    22441 aaccgggcaa tatcgccaat ttttccgccg tatcgggatt tgaggatttg gaagtgttca
    22501 ctgccgtaga tttgttcggt ttcggcgcgg acgacatccc tcatcccgac cggctgccga
    22561 agctgcacag gctgtggatg agcagcctgc ccgaagaggc ggcgaaggca gtgaagaaac
    22621 tttataagaa acggaaggaa gacgggctgg acctgtggat agaaaaagcc cgaaaacccg
    22681 aatggctggc gcaaaatttc gacaacccct tccgcgattg ggacggcgcg gagcatatcc
    22741 cgaaaagcca tgccaaaaaa gcggcggagc tgtaccggaa aacccgtgcc ggcgtggtca
    22801 aactgctcgg caatccgcct gaaaacacag gggaggggct ggcagaggcg gtcaaagcct
    22861 ataccggagg tttcaacaaa atggacaaaa aacactttat cgacacggta gagcgggaag
    22921 atattgccga ggcgttggaa acgatattgg acctgatacc ggatgggtct tgtgccgaca
    22981 aggaaaaact gtttgaaata tttgataaaa acaggaattt ttaaacagag gcaatgccgt
    23041 ctgaaacttg gtaacgggct tcagacggca tcccgttcca acaccgccaa caccgccgca
    23101 ccgtaacgtg cggctttttc ttcgcctacg ccgtatacgg cggcaagctc cgccaaacct
    23161 tccggctgtt tggcggcaat ggcgcgcagg gcggctttgc tgagaatgcg gtagggttcg
    23221 gactgttcgt gttttgcggt ttcgttgcac cattgaatca gggcgcgcat caggcagcgt
    23281 ttgtgtttgg cggtttcatc gatgccgtct gaaaacggac ggcagacggc gaggatgtcc
    23341 cgtccgtatt tggcggcgcg tacgctgccc aagccgtgta cggcttcgag gtcggtttcg
    23401 gtttcgggcg tggcggcaag catatcggca aggctttcgt cggagaggac ggtatgcagg
    23461 gcgcagtttt ccgcccttgc ctgttcatac cgccaggctt cgagtttttg acgcagttgt
    23521 tgttcgcgtt cggtttgcgg acggatgacg gcatcgtggc taaagtcggc agcgttttgg
    23581 cagactttga gtataccgtg tccgaaacgt tcggttttgg cttcgcccaa gccgtagatg
    23641 tcgtgcagcc cgttgaggtt ttgcggcatt ttttcgacaa ggtcgcgcag ggttttgtcg
    23701 ccgaaaatca tataggcggg gatgccttcg gcttcagcct gtttcatgcg ccaaacgcgc
    23761 aatgcctgcc acaggcgttc ttcgcgttcg gtacgcagcc agttgtcttt gagggtgcgg
    23821 gtggcgggct tgtcgcgctt gagcgggcgc agcatcactt cggtttcgcc tttgaggact
    23881 tttttggcgg cttcggtcag ttgcaatgcc tgatatcggg caatgttgac ggtgaggtag
    23941 ccgaggctga tgcactggcg gatgacgctg cgccattctt tgtcggacaa ttccgcgccg
    24001 atgccgaatg tggacagttg ctcgtgccgg ttgccgcgta tccaatcgtc gcttttgccg
    24061 cgcaaaaggt tggtgatgta accggcggca aagcgttgtc cggcgcggta cacgcagctg
    24121 agtaattttt gcaccaacac cgtgccgtca aaccgtacgg gcggatgcag gcagttgtcg
    24181 caatgtccgc agggttcgga tgcttcgccg aaatgtttga gcaacagtac gcggcggcag
    24241 gcggcggttt cgcagacgga aagcatggca tcgagttttt gcatttcgat ttgcttttgc
    24301 acctcgtcgc tgttgccttc ggcaatccgt tcgcgcagca acacccaatc gttcaagccg
    24361 taacacagcc aactcacggc aggcagcccg tcccggccgg cgcgccctga ttcttgatag
    24421 aaatgttcga cactctgggg catatcgaga tgggcgacaa agcgcacgtc gggtttgtct
    24481 atgcccatgc cgaacgccac ggtcgccacc acgataatat tgtcttcatg cgtaaagcgg
    24541 cgttggtttt cctcgcgcac gtccatgctc aaaccggcat gatacggaat cgcgtttaat
    24601 ccgttttcac gcaaaaactg cgccgcatct tcaacctttt tgcggcttag gcaatacaca
    24661 atgccgcttt gccccgtcat ttctttgcgg atgaaatcca gcagttgttt tttgccgttg
    24721 tttttttcga taacctgata ataaatattc gtgcggtcaa agctggagac aaattcggac
    24781 gcgtcgtcca agtgcagata atgcttgatg tcggcgcgcg tggcggcatc ggcggtggcg
    24841 gtcagggcga tgcgcgggat gttcggatag cgttcggcaa gcataccgag ctgttgatat
    24901 tcggggcgga aatcgtgtcc ccaccggctg acgcagtgcg cttcgtcaat ggcgaacagg
    24961 ctgacggttt gttggtcgag aaaacgtaaa aagcggtcgg taaccaagcg ttccggcgcg
    25021 acataaagca gcttcagacg gccttgggca agcttgtcgg caatctcgcg cgcctcatct
    25081 gccgatgtgc cgctgttgac tgccgccgct tcaatcccgg ccacatgcag gctggccact
    25141 tggtcgttca tcagcgcaat cagcggcgat acgacaaccg ccacgccttc gcgcatcagc
    25201 gccggaatct ggtaacacaa agacttgccc ccgcccgtcg gcatcagcac cgtcaaactg
    25261 ccgccgcctg ccaaagtatt gatgacatcc tcctgcctgc cgcgaaattc gggatagccg
    25321 aatacttcgt gcaaaatctg tctggcggtg ggtcggtgcg tcattgttcc gtgtccggta
    25381 agggtgttgg tcggtcggcg gcaatatgcc gtctgaaatc gggattttag aagagtttgc
    25441 ccacttccgc cccaatatcg tcggcacgca taaacgtttc gccgatcagg aaggtatgca
    25501 cgccgcgcgc ccgcataaat tccacatccg ccttgcctgt aatgccgctt tcggtaacga
    25561 cggttttgcc ttccagcgcg ggcagcagcg acagggtttg gtcgagggag acttcaaaag
    25621 tccgcaggtt gcggttgttt acgccccgca acggcgtggt caggttgcgg catttttcca
    25681 attcggtttc gtcgtgcagc tcgagcagga cggtcatgcc caattcgtgc gccagcgcct
    25741 caaagcgttc caattgtccc tgttccagtg cggcggcaat cagcaggacg gcatccgccc
    25801 cccatgcgcg cgcctgataa acctggtatt cgtcgatgat gaagtctttg cgcagcacgg
    25861 gcagcaatac ggcttcgcgc gcctgtttga ggtattcggg cgaaccttgg aaatagggtt
    25921 cgtcggtcag taccgacaaa cacgccgccc cggcgttttc ataggcgcgg gcaatctcgg
    25981 cagggcggaa gtcggggcgg attaaccctt tgctcgggct tgcctttttg atttcggcaa
    26041 tgacggcggg caggtttagg cggtgtttgc cgcgtatcga atcgatgaag ctgcggacgg
    26101 gcgcggcttc tgcggcaagt gcgcggatgt gttcggcgtt gacggcggct ttttgggcgg
    26161 caacttcctg tgccttggtg gcaaggattt tattgaggat gtcggtcatg tcgtgttccg
    26221 tatttgtccg gggaaagggg gaatattagc atcaaaccgt taacgcctgt ttgtgcggaa
    26281 gctgtcaaaa taggacagga cggtctgcgg cagccattgc aggtgcagcc tgccgccggt
    26341 gctgctgaca aagccggcgt gcccgccgtg tgcaggttgg aacagggtaa cggcttcgga
    26401 cacttcgtcc gcacggggca gggcttcggg cggcaggaag gggtcgttgg cggcattgag
    26461 caggagcagc ggtttggcaa cgtgtttgag cagcggtttg caggaagttt ggcggtagta
    26521 gtcgtgccgg tcggcaaagc cgtgcagcgg tgcggtgaaa cggtcgtcaa actcgcccag
    26581 tgttttgcac cctgcggcaa atgccgtctg aaaaccttgg agcgaacgtg ctttgggtat
    26641 cagtgtgcgg aggaagtagc gcgtgtagag cagccgcgtg atgccgctgt cgaagcggct
    26701 gcctgccgcc tctgcatcaa cgggggcgga tacggcggcc gaggcgtgcg gcaatgcctt
    26761 tttgccctgt tcgcccaaat attttgccgg cgcgttgccg cccagcgata cgccgacggc
    26821 gtatatttca cggtaacgcg cggtgagcgt gtccaaagca aaggcgattt cggcggtatc
    26881 acccaagtgg tagaacaccg gggcggtgtt cgctacgccg ccgcagctgc ggaaatggac
    26941 gactgcgccg tgccaacccc gattgcgtac cgcgagcatc agttcggccg cgtaatggct
    27001 gcggctgctt ccttccaaac cgtgaaacag cacgaccagc ggcgcatcgg gcgaaatgcc
    27061 gcctgaaaag tcgtaggcgg ttttggtttt acccgtgctg tcgggaagca tctcgcggcg
    27121 gtatgcgggt gcggggtgtt gcaggaattt ggcggcaatc gtgtcggcat tgccgttgcg
    27181 gaggaaaaag ggcgtgtccg gcggtgtcaa aatcataagg tatcggtttt tctgttttca
    27241 gacggcattg gtgatgcggc agcccgtccg gccggtgcgg acgcgggcga tgcgcgcccg
    27301 gatataggcg cggaaaaagc gttttccgaa aaaggatatc ggcatcggtc agctttccac
    27361 gcgtttgaaa tggcgcggac ggaagcccaa agccgccaga gatgcgaaat acagtccgcc
    27421 gccgacggca atcaggatgc agagctgccc cgctttccgc attccgccgg cgtgcgccca
    27481 ttcgaacggc aggcaagcct gcgccgccca cagtccgccg cacatcacgg cgagcgcgag
    27541 cagcattttc gccaagaacg ccgcccaacc cctgccgggc cggtaaatac cgtgtttgcg
    27601 caacaggaag aacaacaatc cggcgttgat gcacgcgccc aggccgatgg cgagcgaaag
    27661 cccggcgtgt ttcaacggac cgataaaggc gaggttcatc aactgcgtgc agatgagcgt
    27721 gaagatggcg attttgacgg gcgttttgat gttttgccgc gcataaaagc cggatgccaa
    27781 cactttgatc ataattaaac cgattaaacc gaaagaatag gcaatcagcg cgtgttgcgt
    27841 catttgtgcg tcaaacagcg tgaattctcg gtacataaac agcgtcgcca ccagcgggaa
    27901 cgacaatacc gccagtccgg ccgccgccgg cagcgtcagc agcatgcaca ggcgcaaacc
    27961 ccagtcgagc agggcggaaa actgttccgt atcttggttt gccgagtgtt tggacaaagt
    28021 cggcagcaaa attgtaccga gtgcagcccc cagcacgccg ccgggcagct ccatcatgcg
    28081 gtcggcgtaa tacatccatg aaacgctgcc cgattgcaga taagacgcga aaatcgtgtt
    28141 gataaccaaa gaaatttgcg ccacgctcac gcccaaaatc gcaggcgcca tctgtttcat
    28201 gacgcggttg accgccgcat ctttgaaatt cagtttgggc agtttcaaaa agcccagttt
    28261 cgccagccac ggcagttgga aaccgagctg caaaataccg ccgacaaaaa ccgcccacgc
    28321 cagcgcggta acgggcggat cgaaatacgg cacgaaaaac agtgcgaata cgataaaaga
    28381 gatgtttaaa aacgtgggcg taaacgcggg aatgccgaac ttatggtagg aattgagtat
    28441 cgagccgaca aaagaagaca aagaaatcaa taatatataa ggaaacgtaa tccgcagcag
    28501 gctgatggaa agttggaact tgtccgcgtc tttggtaaag ccgggcgcgg aaacataaat
    28561 cacccaaggc gcggcaagta tgcccagcgc ggtaacgacg atcagcacaa acgacagcat
    28621 tcccgcaacg tggcggataa aagcctccgt cgcctcttta gaacgcgttt ccttatattc
    28681 cgccaaaatc ggcacaaacg cttgggcaaa cgccccctcc gcaaacacgc ggcgaagcag
    28741 gttgggcagt ttgaacgcga caaaaaacgc atccgtcgcc atacccgcgc cgaatgcccg
    28801 cgcaatgacc gtatcgcgca caaatcccaa aacgcgcgac accatcgtca ggctgccgac
    28861 ttttgccaaa gctccaagca tattcatcgt gtttcctttt cggttgaaac cccgcccttt
    28921 agggcggcag gatcagactt tatagtggat taacaaaaat caggacaagg cggcgaagcc
    28981 gcagacagta caaatagtac ggcaaggcga ggcaacgccg taccggtttt tgttaatcca
    29041 ctatatttga aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat
    29101 gtgccgtcct gtgtgttgaa acatcattgt tccccaacag ccgtatccgt ccggggcggc
    29161 gcgtattgta cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt
    29221 ttcccgaaac gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat
    29281 aaaataccaa tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc
    29341 tcgccctcgg cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca
    29401 gcgtccccgc cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg
    29461 aagggcaaaa ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg
    29521 aagtgcttga gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga
    29581 gcaaacacgc caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc
    29641 agaaagaaat gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa
    29701 gcaaagatgt ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc
    29761 aagagccgga agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag
    29821 tccttgccgc ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga
    29881 ccgaaacctt ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagttg
    29941 aatttgccga ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg
    30001 aagaacaaaa agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca
    30061 gacggcattt tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca
    30121 gttcgaccaa gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca
    30181 gatagtttaa gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca
    30241 ggcgtgcgaa gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc
    30301 ggtaaaactc gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt
    30361 agcggataac caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca
    30421 gcgacaccaa atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg
    30481 gcctgccgcg cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg
    30541 gctgcgccaa caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt
    30601 tgaatgttaa ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt
    30661 cgcgcagcat cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca
    30721 atccgaccag ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct
    30781 cctgaagcat cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca
    30841 aatccgtgtc cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct
    30901 tcaaataagg cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc
    30961 cgtctgaaaa cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca
    31021 gatcgaagtc ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt
    31081 gcataacagt atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac
    31141 ttgaggggcg attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt
    31201 ctgtttcggc acgcattgcg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt
    31261 cgcagaaggc ggtgcgcagg gcgcgtccga atccgcacaa gcttccgatt tgaccctcgg
    31321 ttcgacctgc ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt
    31381 ccaaggcagc ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat
    31441 ggaaggacag tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg
    31501 cgcagtcctc aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg
    31561 cgaccggttc gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa
    31621 tctcgatcag cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg
    31681 gcggctgcaa agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac
    31741 ggaaacccaa ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc
    31801 tgtcgaagcc gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg
    31861 cggcgttccc cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag
    31921 cggactgctc gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta
    31981 ttatttcaac cttgccccca acttcgatgc cactttcgcc cccggcatta tcggagaacg
    32041 cggcgcgacg tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga
    32101 cctgacctgg ttgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg
    32161 gcagcaccgg cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc
    32221 cgacagcggc tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct
    32281 caaccgccgc gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg
    32341 cctttcggtt cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta
    32401 cgccatcatg ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg
    32461 cgtgtccgca caatttaccc gcttcagcca agacggccgc caagacggca gccgactggt
    32521 cgtgtatccc ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact
    32581 ggggctgcac gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt
    32641 cgggcgcgtt ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg
    32701 cctgttcggc ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc
    32761 tgccaaatct caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg
    32821 gcagcttttc cgcgaaaacc tttattacgg caacgaccgc atcaacgccg ccaacagcct
    32881 ttccaccgcc gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc
    32941 cggtatcggt cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg
    33001 caaaaatccg cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg
    33061 tttcaccctc gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc
    33121 cgtcggcgca ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg
    33181 gcgcaacgaa aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca
    33241 gctcgacctg tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa
    33301 ctacggtttt gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag
    33361 ttgcggctgc tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta
    33421 caaaaacgcc gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc
    33481 cgcaggcagg atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg
    33541 acgcaacaaa cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata
    33601 gcattccacc gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga
    33661 atgatgaaaa tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac
    33721 gccgcaccgc aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg
    33781 caaaaagaag ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc
    33841 gccgttgccg acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc
    33901 aaagccaccc tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg
    33961 atgcagcttg tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca
    34021 agtgaagcgg aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc
    34081 acccaacgcc gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg
    34141 atgcaaaaca gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa
    34201 caaggcatca ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg
    34261 attaaagccg acagcaaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac
    34321 gaccaggcgc gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca
    34381 agcgcgggca atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt
    34441 gaagaagccg tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc
    34501 ggctggcaca tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc
    34561 cgcaattccg tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg
    34621 ttgcgcgacc tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa
    34681 gtcataccga tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt
    34741 acaccgccat tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt
    34801 tatcgggaat ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca
    34861 gaagttacac gaaactcaaa aaaaaaaacg aaaccgaacg aaccggattc ccgcttttac
    34921 gggaatgacg gcatatcggt ttccgtgagg acaaacctag attcccgcct gcgcgggaat
    34981 gacgggattt tagattgcgg gtatttatcg ggaatggcga tttaaaaatt acctaaaact
    35041 aaacttaaaa gcaactgaaa cttaaaaaac taaaatttcg cctgcgtggg aaggatggga
    35101 tttcagattt ctgtttttgt gggaatttgc cgtatcgggc ttcagacggc attgcctgcc
    35161 gttttgcccc cgggtgcggc tgccttgatg tagttgagcg agacaaactg cttctcggca
    35221 tccaattcgg tgattttaaa caatgcctgc gatttgggca gcgcgtcaaa cgggatgccg
    35281 ttggcgcgcg ttgtcagtgg cagaccttcg atgcggacga ggtcttcttt cagaatcgtc
    35341 gcggtcagtt cgctgatgcc ttgttgttgc aggtacacga ggctccagta ggcttccatc
    35401 tgccgttgga agtcggcgta ggcggcgtag gcggtgtcga aatcgcgcag ggcggcgaag
    35461 agcgcggcat cgcttttttg atacagcggc tcggcagtgt cgtcgatcag gctgatcagc
    35521 tgcttttggt tgatgtagtc ggcggcgcgg cgcagcggcg aggtaaacca gccgtaatgc
    35581 tgcacgccca tgccgatgtg cggctcggat tgggtgctca tgcgcacttt gcctgccggt
    35641 tggacgcgga aaaggccggg cagcccgttt tcgtctagca tttgcgccca agtgctgttg
    35701 gcaagaatca tcatctcgct gaccagcgta tcgatgggcg aaccgcgttc gcggcggacg
    35761 acggagacgt tgccttcttc atccaattcg atgctgtaat cgtattgcgg cgcgcggtcg
    35821 ggttcgtatt tgccgcgcgc tttttgcagg gcggtggcga attgatagaa ccaaatcagg
    35881 tcttgatggt gggcgaacat catttcgcct gcttggtcca agccggtttc tgcgttgaaa
    35941 tgcggctcga tggcttggat acgcaggttt gcggcgatgt tgacggcttc gattttgcag
    36001 gtcggctcgc cgacgttgaa ctcgccgtcc acatcgaaat aaatgctgac ggcagggcgg
    36061 tatgcgcctg catcgaggct gaacgcggta atccagtttt cgggcagcat cgtgattttg
    36121 ccgccgggga aataaaccgt actcaaccgc tgcataatga tttgttccat tccgccgcct
    36181 tgtcggacgg caagcgacgg cgcggcgatg tggatgccga cgcgcttcgt gccgttgccc
    36241 aaatcggtca ggctcaaggc atcgtccact tcggtggtcg attcgtcgtc aatggaaaag
    36301 gcggtaacgt cggccttggg caggtcgggc atttcgggaa gggagaggtc ggggaaacct
    36361 gttcctttgg ggaagtattt gatttcaaag ccgtcttgca ggtattgcgg aatggacgta
    36421 atgccgcccg tttttttcgc cagttcgtag gcggaggttt tcagtgcgtc ggcggctttg
    36481 gtaaaggctt tgtaggtcag cgactgtttg tcgggcgcgt gtaagatggt tctcaaatcc
    36541 gccgcgattt cagacggcat ctcgccgcgt ttcaaggctt ccgcccaagc gtcgatttgc
    36601 gcgtcttgct gttttttgcg ttcgatggcg gcaagcgctt gtttcaaagt ttcttcgggc
    36661 gcggctttga acacgccttt ggcttttttg tagaaataca ccggcgcggc gtacagcgca
    36721 atcaaagtcg ccgccagctc ggttttggtc ggcgcatggc cgtaatattc ttcggcgatg
    36781 gcttcggcgg taaactcctc ttcgccgcat acttcccaca ataaatcggt gtcgatgtcc
    36841 gccgcctgcg cctgcgcgtt ttccaaaaac gccgccatat cgccgtcaaa ctcggcaaag
    36901 acattgtttg ccttcacttt ggtgcgtttg ccgtgttggg tatcgacttg gtaggtagcg
    36961 tcgttttttt ggacgacgac ggcaactttg aactggccgg actcttcgta aaaaatattc
    37021 atttttcgga tttttctgtg gaaactcaag cgggcgattt tagcagatta ccgaaaatgc
    37081 cgtctgaaaa aaggttgggg gaaggcgggt gcggctttgc ggtgcttgcg ttgcgttttt
    37141 gacggggtac aatcgccgtt tttgaacgga ggacggaatg gagaatgtaa accgcgtgcc
    37201 ggagcaggca cgttatgatg ccgaacgcag gcaggcagac gaagcattgg cggacgtgtt
    37261 tccggcagtc agtattttcg gcagcgcgcg cacgccgcag gatcatgcgg attatgcgtt
    37321 tgcctgccgt ctggcgcggc ggctgtcgga ttcgggtatt gccgtcattt cgggcggcgg
    37381 gccggggatt atggaggcgg caaacaaggg cgcgtttgcc gggaagtcgg tttcggtagg
    37441 gctgaacatt gccctgccgc acgagcagaa accgaatccg tatcaggaca tcgccttgcg
    37501 gttttcccgt tttgccgaac gcaaggcagt gtttttccgc tattcccaag catatgtcgt
    37561 gatgccgggc ggcttcggga cgctggacga attgtttgaa atcctgacct tggtgcagac
    37621 gggcaaagtg ccgccgcgtc cggtcgtttt ggttggaaag gcgttttggt cgggcttggc
    37681 ggagtggata aacgcgcagc ttttggcgcg cggtatgatt tccgaagggg ccgcttcttt
    37741 gttctccata tcggacgacg aagacgaaat cattgcgtat ctgtcggaac acgggcttca
    37801 gacggcatag cgtcctgaga gtgatgtata attgcaaaca atttgtcaat ttttgatgtc
    37861 ttttccgaac aggatgccga tatgatcaac cccatcgcct cgctttcccc cctagacggc
    37921 cgttatgccc aatccgttga agcattgcgc ccgatttttt cagagtacgg actgatgaag
    37981 gcgcgcgtca aagtcgaatt aaactggctc aaagccctgg ccgccgcgcc ggagattgcc
    38041 gaagtgccgc ccttcagtgc cgaaacgctt gccgaaatcg acaaagtcat cgaaaacttt
    38101 tcattagaag acgcggccgc cgtcaaagcc atcgaagcca cgaccaatca tgatgtcaaa
    38161 gccatcgaat attggctgaa aaaacgtttt gccgaagtgc cggaagtcgc cgccgtgagc
    38221 gagttcatcc acttcgcctg caccagcgaa gacatcaaca acctgtccca cgccctgatg
    38281 ctgcaagagg cgcgcgaaac cgtcatcctg cccaaacttg ccgaaatcat cggcaaactc
    38341 accgctatgg cgcacgacct tgccgccgtc ccgatgatga gccgcaccca cggccagccc
    38401 gccacaccga ccactttggg caaagaaacc gccaatgtcg tgtaccgcct gcaacgccag
    38461 ttcaaaatcc ttcaggcgca agaattcctc ggcaaaatca acggcgcggt cggcaactac
    38521 aacgcccaca tggccgccta tcccgatgtg gattgggaaa cacactgccg caacttcgtc
    38581 gaaatcagcc tcggcctgac cttcaacccc tacaccatcc aaatcgagcc gcacgactat
    38641 atggcggagt ttttccaaac cctcagccgt gtcaacacca tcctgatcga ctttaaccgc
    38701 gacgtttggg gttatatttc attgggttat ttcaaacaaa aagtcaaagc gggcgaagtc
    38761 ggttcttcca ccatgccgca caaagtcaac cccatcgact ttgaaaactc cgagggcaac
    38821 ctcggcatgg caaacgccgt attgggcttt ttgtccgaaa aactgccgat ttcccgctgg
    38881 cagcgcgacc tgaccgacag caccgtattg cgcaatatgg gcgtgggcgt gggctatgcc
    38941 gtgttgggtt tcgccgccca cctgcgcggt ctgaacaagc tcgagccgaa ccctgccgcg
    39001 cttgccgccg atttggatgt cacttgggag ctgctcgccg agccgattca aaccgtaatg
    39061 cgccgttacg gtgtcgccaa tccttacgaa aaactgaaag acctgacgcg cggtaaaggc
    39121 ggcatcacac ccgaagtgct gaaaggcttt atcggatcgc tggaaattcc cgccgaagcc
    39181 aaagccaaat tgcttgagct gacccccgcg ctgtatgtgg gcaaggctga agcgttggcg
    39241 aaacggattt gagcgtttac tgaaaccgat gccgtccgaa cgcgcgttca gacggcattt
    39301 ttaagataac gggacatacg gggcgatatt gatgcaagct gtccgataca ggcctgaaat
    39361 tgacggattg cgggccgtcg ccgtgctatc cgtcattatt ttccacctga ataaccgctg
    39421 gctgcccgga ggattcctgg gggtggacat tttctttgtc atctcgggat tcctcattac
    39481 caacatcatt ctttctgaaa tacagaacgg ttctttttct ttccgggatt tttatacccg
    39541 caggattaag cggatttatc ctgcttttat tgcggccgtg tccctggctt cggtgattgc
    39601 ttctcaaatc ttcctttacg aagatttcaa ccaaatgagg aaaaccatag agctttctac
    39661 ggtttttttg tccaatattt atttggggtt ccgattgggg tatttcgatt tgagtgccga
    39721 cgagaacccc gtactgcata tctggtcttt ggcggtagag gaacagtatt acctcctgta
    39781 tcctcttttg ctgatattct gttacaaaaa aaccaaatca ctacgggtgc tgcgtaatat
    39841 cagcatcatc ctgtttctga ttttgaccgc atcatcgttt ttgccggccg ggttttatac
    39901 cgacatcctc aaccaaccca atacttatta cctttcgaca ctgaggtttc ccgagctgtt
    39961 ggtgggttcg ctgttggcgg tttacgggca aacgtaaaac ggcagacggc aaacagaaaa
    40021 tggaaaacgg cagttgcttt cattactctg tttcggcgca ttgcttgtct gcctgttcgt
    40081 gatcgacaaa cacgatccgt ttatcccggg aataaccctg ctccttccct gcctgctgac
    40141 ggcgctgctt atccggagta tgcaatacgg gacacttccg acccgcatcc tgtcggcaag
    40201 ccccatcgta tttgtcggca aaatctctta ttccctatac ctgtaccatt ggatttttat
    40261 tgccttcgcc cattacatta caggcgacaa acagctcgga ctgcctgccg tatcggcggt
    40321 tgccgcgttg acggccggat tttccctgtt gagctattat ttgattgaac agccgcttag
    40381 aaaacggaag atgaccttca aaaaggcatt tttctgcctt tatctcgccc cgtccctgat
    40441 acttgtcggt tacaacctgt attcaagagg gatattgaaa caggaacacc tccgcccgct
    40501 gcccggcgcg ccccttgctg cggaaaataa ttttccggaa accgtcttga tcctcggcga
    40561 ttcgcacgcc ggacacctgc ggggttttct ggattatgtc ggcagccggg aagggtggaa
    40621 agccaaaatc ctatccctcg atccggagtg tttggtttgg gtggatgcga aactggcaga
    40681 caatccgtta tgccggaaat accgggatga agttgaaaag gccgaagctg ttttcattgc
    40741 ccaattctat gatttgagga tgggcggcca gcccgtgccg agatttgaag cgcaatcctt
    40801 cctgataccc gggttcaaag cccgattcag ggaaaccgtc aagaggatag ccgccgtcaa
    40861 acctgtatat gtttttgcaa acaatacatc aatcagccgt tctcccttga gggaggaaaa
    40921 attgaaaaga tttgctataa accaatacct ccggcctatt cgggctatgg gcgacatcgg
    40981 caagagcaat caggcggtct ttgatttggt taaagatatt cccaatgtgc attgggtgga
    41041 cgcacaaaaa tacctgccca aaaacacggt cgaaatacac ggccgctatc tttacggcga
    41101 ccaagaccac ctgacctatt tcggttctta ttatatgggg cgggaatttc acaaacacga
    41161 acgcctgctc aagcattccc gaggcggcgc attgcagtag cctgccttct tgtcggatat
    41221 tgcctttggc agcctatgcc gctgtttgcc ctttgaggcg gcttttattt tgccgttttg
    41281 aggccggggt cgggaataac cgttttttga tgattttccc tccccggctg tgtcatcaaa
    41341 tccctagttg cctttccaaa ctctccacca gattgtcatc cagtttcaaa gcctgcgaca
    41401 ggcgggcgag gaagacggtt tctttccgcg acaaatcggc acagaccaac cttgccgcca
    41461 gataggtctc cgccgccaac gcctcatcgt tgccgacggc ggcggcgatg tccccgatgc
    41521 ttgcgggcag gcggtattcg gcggcgagcc atgcggcagt ttcggggtct gtgccgcttt
    41581 cctgttcgat agtccggcgt tcggcttcgt ctatcatgcc gtctgaagcg gcggcggcta
    41641 tcatggtacg caatacggtg cggctgtatg tttcttcggt ttttccggca ggttggaaat
    41701 cgctttgtgt tacggttgcc cgccctttgt tttgctgcca catctgatag ccccggtagg
    41761 cgaggtagcc caaagcggcg gtcgaaccga ttttggtgat ggttttgcgg tttttaccgt
    41821 tcagcagcat ggaggcgaca ccggcaacca gcgcgcctcc gccgaatgaa ttgagcgggc
    41881 tgtcggagaa tgtgctgcct tttttttgaa ccgtgcttaa gacttggttg agcaaccggg
    41941 tgaagttcat gatttttcct ttctgttgcg ggaagggcgg tatgtttacc ctatcctttt
    42001 aaacggtggc aggccggcca ataattgttg gccgtaccgc tgcgttttga tgcggttgtc
    42061 gaggatggtt acgcggccgt agtcttgttc ggtgcggatg aggcggccga cggcctggat
    42121 gagtttgatg ccggcttcgg ggacggtgat ttcgatgaag gggttgccgc cgcgttgttc
    42181 tatccaacgg ttttgggttt tttcgatggg gttgtcgggc atggcgaagg ggagtttggc
    42241 gatgatgact tgcacgcagg cggtgccggg caggtcgagc ccttcggcga agctgtcgag
    42301 tccgaagatg atgctggctt tgccttcttc tatggcttgg tggtgttttt gcaggaggac
    42361 ggctttgggt aattcgcctt gtacgagcaa gagcggcagg tagtctccgg gcaggtgcag
    42421 ggcgacatcc tgcatttgtt tgcgcgagga aaacaggacg agtgtgccga tggcttcggt
    42481 gggcgaaatc agcttgggca gccattcgat gacggcggcg gtgtgggctt cggggtcttt
    42541 ggggctggcg tatatggagg ggatgtagag ttcgccctgt ttttcaaagt caaaggggct
    42601 tttgagggcg agggtggtgg tttcgggcag ccacagcagg ccggtttggc gcaaaatcaa
    42661 gttgaagctg ccgagcgatt gcagggtggc cgaagtcagc accgcgcctg ccgcgcgccg
    42721 ccacaggctg ttggcaaggt gggacgcgct gctgatgggg ctggcattga aaatatagtc
    42781 gtttttgtcg tcggcacggc gggctatcca tttcgccaac ggctcctcgc cctcaatgga
    42841 aacagtggag agcaaatccc aaaccgcgct gatttgttcg atacgggcga taaaaaggcc
    42901 gaactcgccg gtcaggcggt cgatgagcgc accgtcctgt tctttttcgc ggcgtgcggc
    42961 ggaaagcgca tcgttcagcc cgacaacgtg tttgagcagg ctgcgtgcag caatggccgt
    43021 attggaaacg gtggtttcga ggccttcggg gattttgccg tcttcccaca gccaagtcgg
    43081 ttcgctgttg gttcgtctgt cgttttcaga cagccccata cttaaagacg gctcttccgc
    43141 caaatggaat tgccattcgt gcaggctgtc gagcaaggat gcggcggctt cgtcggcaag
    43201 gttggcaagt tcggctttat cggtaagcgc ggcaattttg ccggtaagct gcggcagttt
    43261 ttccagcgtc caaacggcaa tattccatga atgttcggcg gcaaaacggc tgagggcttt
    43321 tttgggcagg tggtgcgctt cgtctatgca atagaaactg ttttcgggcg caggcagaat
    43381 cacgccgccg cccatgctga tgtcggcaag cagaagatcg tggttggcaa caacgacatc
    43441 gacggtttcc aacatatcgc gtgccaggta aaaagggcat tccggacggt tgggacaggc
    43501 ggctttcagg cagccgtggc ggtcgttggt cactttgagc caaatcgcgt catcgatttt
    43561 ttccggccaa gcgtcgcggt cgccgttgaa ccgtcgggcg gaaaattcgt cggcgatgtc
    43621 gcgcagcagc ttcaattctt ccggcttggg tttgctgtcc cacaagacgg cgggggcttc
    43681 aaagccgagc aggttttgct gggcgttgct ttgcgtcagt tgatagagtt tgtaggggca
    43741 gagatagcgg ccgcgccctt tggcaagtgc gaaggtcagt tccaaaccgc ttttttcgac
    43801 cagaaacggc aggtcgcggt ctaccaactg ctcctgcaag gcaaccgtcg cgctgctcac
    43861 aatcagccgc ttgccgcgtg tttgcgccat gatgccgccc gcaagcaggt aggcaagcga
    43921 tttgcccacg ccggtcggcc cttcgattac ggcaatgctt tcgccctcgc gcttgggcgg
    43981 ctcgccgcct tcttcgcgcg tcaacgtccg cgaaaaagcg ttggcaaccg ccgcaatcat
    44041 ttcccgctgc gaagcacgcg gacggaaacc gggcaggttt ttgccgatat tttggtaatg
    44101 gtcgcggatg gcgttttttt ctaaatcggt gagcatagta tcggtgggcg gatgatttgg
    44161 ggtcatttta acattgcgcg gatgcggtac ggtatcgttg tcggacggtg gttcgggcat
    44221 atctgattat tgaaagtaaa aaaacggaag atattgggtc atccgtacaa aatggcgtct
    44281 gaagcgggaa acgggcttca gacggcattg ccgcgcttat acgccggcga cggggcgggc
    44341 gggatagccg tgggtatcga ccgaacaggc aaaccgccaa ggcgtgtgga cggtgtcggc
    44401 ggacaggtgg gcgagttcgg gaatgtgccg tttgataaaa gtaccgtccg ggtcggtacg
    44461 gtatgaggcg gcggcaatgt cggggcaggt gtgccgtgag gcggcaagcc gccagttgcc
    44521 ttggttgatt gccgcatcga aatcggtcag ctgccgggca aaccatatct cgccttcgcg
    44581 gcgggggagg tttaaaacgt ggcagaaaaa atccgcgctc aagcgtctca gggcggggtg
    44641 gaggctgccg gttttgtgca aacagcgcat cgcggcatcg ataatcggaa tgccggtccg
    44701 gccctgctgc caaagcgtca ggcgcagggt gtgttccgga ttgccgtctg aagggtcgtc
    44761 atccgcgtgc tgcaaggcaa gttgaaggaa aaaatcgcgg cggatgatgt tgtccgccca
    44821 cgcgttcaga cggcgttcga ggctttcccg cgcgagcagg cgcggcgaga tgcagccgac
    44881 actcaaatac gcgcccatca gcgaagtgtt tttgcgcgaa gggaaatcct ttaaaacgga
    44941 gtaggaagcc gcctgttcga gaaaccgccg ccactgccgc caagccgccg tttcgccgcc
    45001 gttttgcggc aggaagatgc cgtctgaaag cgcggcaggc ggcggggcgg gcaggttttc
    45061 ggggaagggt tggcggtatg ccgcgaatag gtccgaaccg gcggggggct gcttggaaaa
    45121 gcggtcgagc catacttcgc ggtagcggtt gaaatcggtg tgtgccgtac cgttgtctgg
    45181 tgtcaggtcg gttttgccga aaacggaacg gtcgttgacg aaggttaacg cgatgccgtg
    45241 tttgtccaat tcgtgccaaa gggcgttgtc ggcgagtttg tcggcaaaag tatgggattc
    45301 gtcggcgatg acggcgcgga tattgaggcg gacggcgagc cggacaagcc cggcaggcga
    45361 tgccgccgtg tagagcggga tgccgcgcct tgccagccct tgggcgagtt cggcggcgga
    45421 ttggcggtag aacgcggcgc ggcgcgggtt gtctgtttcg gcatcgtcaa tccaaatgcc
    45481 gataacgggc gaaccttcgg caacggcggc gcataaggcg gcgttgtcgc ggatgcggag
    45541 gttttggcgg aaccagacga gcgtgtgcgc ggcgcgcgtg tccgcataaa ggaggagggc
    45601 ggtttcagac ggcatttcgg cagcctttcc tgctggcgat tttttcgttc agaaaatcga
    45661 tgaaactgcg gactttcgca ctcaaaaatg ccctgtccgc ataaacggcg ttcagccggt
    45721 cggtcgggac ggcgtatccg ggcagcagcc tcaccagcgg gccgcagcgc aaatcgtgtt
    45781 ccgccgccca aagcggctga taaccgatgc acgcgcccgc cttaatcatt tcgcgcatca
    45841 tcagcgtgtt gtcggtacgg atgacggggg tcagttcaag ccggtatttc ttgccgtccg
    45901 atttgcgggt gaggtcgagt ttctgctggt tggtgtaggt cggcaggacg gcaggcagcc
    45961 ccgccacttc ttccggtgtt tccggcacgc cgttgcgcct caggaagtcg ggcgaggcaa
    46021 gcagggcaaa ttcgatttcc gccagcgggc gcgcaatcag cgaaggggaa agggtttggg
    46081 aaacgcgcaa cgccaaatcc acgccttcgg caatcaaatc gacgtggcgg ttgtccaaaa
    46141 tcagttccaa cgccacttcg ggataacgtt cgcggtattc cgccagccag ttgcatatct
    46201 ggctgccggc aaaccacagc ggcatcgtta cgcgcagcag cccctgcggt ttttccgtcc
    46261 ccccggcggc tttttgcgcg gcatcgtcga gcgtgtcgag cgcgtaactg cattgccggt
    46321 agtattcttc cccggcttcg gtcaggctga ggttgcggct gttgtggtgc aggagtttgg
    46381 cttggacggt gttttccaag tgactgacgt gtttgcttgc cattgcggtg gagatgccga
    46441 gcgcgtcggc ggcgcgggtg aagccgccgc tttggacgac ttggcggaaa actttgaggc
    46501 tgaacagggt gtccatattt tcttgtgtgg aaaagttgta tcaataaaag cagtatatat
    46561 ttgaaaaagg gaaacatcta tactctgccg cctgaaatga agacaaatat caaaggagct
    46621 tttatgtccg attgctgcaa ccgtatccaa ccggttttgc tttctgtttt gcgtatcgta
    46681 accgcctacc tgtttttgtt gcacggtacg tcgaaaatct tcgccttccc cattgaaatg
    46741 ggcagcggtt cgcccggcgg gctgttgctg cttgccggta ttttagaaat tgtcggcggc
    46801 attttgctgg tgttgggcct gtttgcgcgc cctgccgcgt ttgttttgtc cggccagatg
    46861 gcggttgcct attttatggc gcacgcttcc ggaaatgctt tgttcccgat tgccaacggc
    46921 ggcgagtccg cagtgctgtt ctgcttcgta tttctctata tcgcggcagc gggcggcgga
    46981 gcatggtcgc tggacaggct gtttttcaag cgtaaagcct gaaccggact gcctaaagtg
    47041 tattttgttg aatgtttttg aggaaaagaa atgagccgtc aatccctgca acaggctgcc
    47101 gaaagccgcc gttccgttta ttcgttaaat aaaaacctgc ccgttggcaa agatgaaatc
    47161 gtccaaatcg tcgaacacgc cgttttgcac acaccttttt cgttcaattc ccaatctgcc
    47221 cgcgtggtcg tgctgtttgg cgaagag
//
LOCUS       DAXFWM010000007        45805 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000007 DAXFWM010000000
VERSION     DAXFWM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 45805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45805
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.007"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(143..862)
                     /gene="pyrH"
                     /locus_tag="ACFI8B_000353"
     CDS             complement(143..862)
                     /gene="pyrH"
                     /locus_tag="ACFI8B_000353"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC3833657.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1079..1933)
                     /gene="tsf"
                     /locus_tag="ACFI8B_000354"
     CDS             complement(1079..1933)
                     /gene="tsf"
                     /locus_tag="ACFI8B_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC3833658.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFIRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2062..2790)
                     /gene="rpsB"
                     /locus_tag="ACFI8B_000355"
     CDS             complement(2062..2790)
                     /gene="rpsB"
                     /locus_tag="ACFI8B_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC3833659.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2963..3196)
                     /locus_tag="ACFI8B_000356"
     CDS             complement(2963..3196)
                     /locus_tag="ACFI8B_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833660.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            3274..3855
                     /locus_tag="ACFI8B_000357"
     CDS             3274..3855
                     /locus_tag="ACFI8B_000357"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC3833661.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3916..4506
                     /locus_tag="ACFI8B_000358"
     CDS             3916..4506
                     /locus_tag="ACFI8B_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC3833662.1"
                     /translation="MSERIILSVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            4890..6356
                     /locus_tag="ACFI8B_000359"
     CDS             4890..6356
                     /locus_tag="ACFI8B_000359"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC3833663.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(6543..6914)
                     /locus_tag="ACFI8B_000360"
     CDS             complement(6543..6914)
                     /locus_tag="ACFI8B_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3833664.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(7152..7451)
                     /locus_tag="ACFI8B_000361"
     CDS             complement(7152..7451)
                     /locus_tag="ACFI8B_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3833665.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            complement(7550..8329)
                     /gene="map"
                     /locus_tag="ACFI8B_000362"
     CDS             complement(7550..8329)
                     /gene="map"
                     /locus_tag="ACFI8B_000362"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC3833666.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(8369..9019)
                     /locus_tag="ACFI8B_000363"
     CDS             complement(8369..9019)
                     /locus_tag="ACFI8B_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833667.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPPPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNVPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(9027..9635)
                     /locus_tag="ACFI8B_000364"
     CDS             complement(9027..9635)
                     /locus_tag="ACFI8B_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC3833668.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(9699..10292)
                     /locus_tag="ACFI8B_000365"
     CDS             complement(9699..10292)
                     /locus_tag="ACFI8B_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC3833669.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            complement(10297..10644)
                     /locus_tag="ACFI8B_000366"
     CDS             complement(10297..10644)
                     /locus_tag="ACFI8B_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC3833670.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            10691..11566
                     /gene="rsmI"
                     /locus_tag="ACFI8B_000367"
     CDS             10691..11566
                     /gene="rsmI"
                     /locus_tag="ACFI8B_000367"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC3833671.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(11601..12131)
                     /locus_tag="ACFI8B_000368"
     CDS             complement(11601..12131)
                     /locus_tag="ACFI8B_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC3833672.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            12410..13564
                     /gene="obgE"
                     /locus_tag="ACFI8B_000369"
     CDS             12410..13564
                     /gene="obgE"
                     /locus_tag="ACFI8B_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC3833673.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13618..14565
                     /locus_tag="ACFI8B_000370"
     CDS             13618..14565
                     /locus_tag="ACFI8B_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3833674.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIILETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            14562..15614
                     /locus_tag="ACFI8B_000371"
     CDS             14562..15614
                     /locus_tag="ACFI8B_000371"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFC3833675.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGGESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            15630..17051
                     /gene="cysS"
                     /locus_tag="ACFI8B_000372"
     CDS             15630..17051
                     /gene="cysS"
                     /locus_tag="ACFI8B_000372"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC3833676.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            17222..18001
                     /locus_tag="ACFI8B_000373"
     CDS             17222..18001
                     /locus_tag="ACFI8B_000373"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC3833677.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            17988..18332
                     /locus_tag="ACFI8B_000374"
     CDS             17988..18332
                     /locus_tag="ACFI8B_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833678.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            18342..18872
                     /locus_tag="ACFI8B_000375"
     CDS             18342..18872
                     /locus_tag="ACFI8B_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC3833679.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTVFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(18965..20080)
                     /gene="asd"
                     /locus_tag="ACFI8B_000376"
     CDS             complement(18965..20080)
                     /gene="asd"
                     /locus_tag="ACFI8B_000376"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC3833680.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEAGIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            20255..20836
                     /locus_tag="ACFI8B_000377"
     CDS             20255..20836
                     /locus_tag="ACFI8B_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC3833681.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(20898..21752)
                     /gene="folD"
                     /locus_tag="ACFI8B_000378"
     CDS             complement(20898..21752)
                     /gene="folD"
                     /locus_tag="ACFI8B_000378"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC3833682.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            21975..22052
                     /locus_tag="ACFI8B_000379"
     tRNA            21975..22052
                     /locus_tag="ACFI8B_000379"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22010..22012,aa:Pro,seq:tgg)
     gene            22077..22153
                     /locus_tag="ACFI8B_000380"
     tRNA            22077..22153
                     /locus_tag="ACFI8B_000380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22111..22113,aa:Arg,seq:tct)
     gene            22181..22256
                     /locus_tag="ACFI8B_000381"
     tRNA            22181..22256
                     /locus_tag="ACFI8B_000381"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22214..22216,aa:His,seq:gtg)
     gene            22392..22898
                     /gene="rfaE2"
                     /locus_tag="ACFI8B_000382"
     CDS             22392..22898
                     /gene="rfaE2"
                     /locus_tag="ACFI8B_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC3833683.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            22895..24673
                     /locus_tag="ACFI8B_000383"
     CDS             22895..24673
                     /locus_tag="ACFI8B_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC3833684.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            24786..25610
                     /locus_tag="ACFI8B_000384"
     CDS             24786..25610
                     /locus_tag="ACFI8B_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC3833685.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(26116..26328)
                     /locus_tag="ACFI8B_000385"
     CDS             complement(26116..26328)
                     /locus_tag="ACFI8B_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFC3833686.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGCFVV"
     gene            complement(26343..27131)
                     /locus_tag="ACFI8B_000386"
     CDS             complement(26343..27131)
                     /locus_tag="ACFI8B_000386"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC3833687.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(27210..27404)
                     /gene="thiS"
                     /locus_tag="ACFI8B_000387"
     CDS             complement(27210..27404)
                     /gene="thiS"
                     /locus_tag="ACFI8B_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC3833688.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     AETVLHENDKIDIVRPVVGG"
     gene            complement(27826..28443)
                     /gene="thiE"
                     /locus_tag="ACFI8B_000388"
     CDS             complement(27826..28443)
                     /gene="thiE"
                     /locus_tag="ACFI8B_000388"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC3833689.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(28465..29565)
                     /locus_tag="ACFI8B_000389"
     CDS             complement(28465..29565)
                     /locus_tag="ACFI8B_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC3833690.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTLTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(29562..30788)
                     /gene="cytX"
                     /locus_tag="ACFI8B_000390"
     CDS             complement(29562..30788)
                     /gene="cytX"
                     /locus_tag="ACFI8B_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC3833691.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVKLSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAEIPVAVGVTLIGTVLAVMLPVTEYKNFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(30854..30958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            31033..32517
                     /gene="tldD"
                     /locus_tag="ACFI8B_000391"
     CDS             31033..32517
                     /gene="tldD"
                     /locus_tag="ACFI8B_000391"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC3833692.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLCPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            32661..33320
                     /locus_tag="ACFI8B_000392"
     CDS             32661..33320
                     /locus_tag="ACFI8B_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3833693.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            33310..33978
                     /locus_tag="ACFI8B_000393"
     CDS             33310..33978
                     /locus_tag="ACFI8B_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3833694.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            33980..34711
                     /locus_tag="ACFI8B_000394"
     CDS             33980..34711
                     /locus_tag="ACFI8B_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3833695.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            34824..35678
                     /locus_tag="ACFI8B_000395"
     CDS             34824..35678
                     /locus_tag="ACFI8B_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3833696.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTTADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            36095..36916
                     /gene="prmC"
                     /locus_tag="ACFI8B_000396"
     CDS             36095..36916
                     /gene="prmC"
                     /locus_tag="ACFI8B_000396"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC3833697.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            37002..38390
                     /locus_tag="ACFI8B_000397"
     CDS             37002..38390
                     /locus_tag="ACFI8B_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC3833698.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            38391..38615
                     /locus_tag="ACFI8B_000398"
     CDS             38391..38615
                     /locus_tag="ACFI8B_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC3833699.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(39015..39908)
                     /locus_tag="ACFI8B_000399"
     CDS             complement(39015..39908)
                     /locus_tag="ACFI8B_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC3833700.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            39951..42653
                     /gene="ppc"
                     /locus_tag="ACFI8B_000400"
     CDS             39951..42653
                     /gene="ppc"
                     /locus_tag="ACFI8B_000400"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC3833701.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDALTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            42776..43765
                     /locus_tag="ACFI8B_000401"
     CDS             42776..43765
                     /locus_tag="ACFI8B_000401"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC3833702.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            43819..44148
                     /locus_tag="ACFI8B_000402"
     CDS             43819..44148
                     /locus_tag="ACFI8B_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833703.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            44159..44464
                     /locus_tag="ACFI8B_000403"
     CDS             44159..44464
                     /locus_tag="ACFI8B_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC3833704.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            44470..44649
                     /gene="ssrS"
                     /locus_tag="ACFI8B_000404"
     ncRNA           44470..44649
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFI8B_000404"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            44827..45258
                     /gene="rplM"
                     /locus_tag="ACFI8B_000405"
     CDS             44827..45258
                     /gene="rplM"
                     /locus_tag="ACFI8B_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC3833705.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            45271..45663
                     /gene="rpsI"
                     /locus_tag="ACFI8B_000406"
     CDS             45271..45663
                     /gene="rpsI"
                     /locus_tag="ACFI8B_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC3833706.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
BASE COUNT        10230 a        12068 c        13074 g        10433 t
ORIGIN      
        1 agtggattaa atttaaaccg gtacggcaag gcgaggcaac gccgtaccgg tttaaattta
       61 atccactata atctccatat aataaatggc tgtctgaagc ccatatgcga ctatatctgc
      121 cgaccctatg gtcaatcatc aatcagcagt ggaccagtgt gccttcgtct tcgccggtaa
      181 tgacgcgttt gagcgagcct tgtttggcga tgccgaagac gacgatattg agcttgcgtt
      241 cgcggcagag ggcaaaggcg gtcgcgtcca tgactttgag gtttttattc aaggcttcgt
      301 caaaagtaat ggtttcgtag cgtgtggcgg acgggtcttt tttcgggtct gcggtgtaca
      361 cgccgtcgac gttggtggct ttgagcatca cgtcgcagtt catttccgcg ccgcgcaatg
      421 cggcggcagt gtcggtcgtg aagaacggat taccggtacc ggcggcgaaa atcacgactt
      481 tgccttcttc caaatattga atggctttag gacgggcgta agtttcagcg atttgctgca
      541 tagacagtgc ggattgtacg cgcgctttga tgcccaaggt ttcaaatgcg tctttgagtg
      601 ccaaggcgtt catcacggtc gccatcatgc ccatgtagtc ggcagtggcg cggtccatgc
      661 tgcctgcttg tgcagatacg ccgcggaaaa tgttaccgcc gccgacaacg ataccgactt
      721 gcacacccat tttcacgatt tcggcaattt cgccgacagt ttgaacaatg gtatcgtgat
      781 tgatgccgaa cggatcggaa cccatcaggg attcgccgga gagtttcagt aatacgcgtt
      841 tgtatttgat ttgctgtgtc atgggatacc ttgctttctt gaatgtcgtc ggtcggagtt
      901 gggacgtctg aatttcaaat ggtcggatta caatgatatg gtgtgtcgtt ctattattgt
      961 tttgttttca gacggccttt atattcggtc gtcttaaaat aggtaccgta aacggttttc
     1021 tcaaaaaaaa gcaccctgat tcgtttggaa tccaggtgct ttctttttca taagtgcctt
     1081 acactttggc ggctgcggct acttcggctg cgtagtcgac aacggctttc tcgataccgt
     1141 cgcctacttt gtagcggatg aagctaacca cttcagtgtc gttttcttta gcgaattggg
     1201 caacagtttg gtcagggttc atcacgaatg cttggccgtt cagagtgatt tcagccaaga
     1261 atttgcggat acggccttca accattttag cggcgatgtc ggcaggtttg ccggaagcga
     1321 tggcttgctc ggtgtagatg tggcgttctt tttcaacggt ttcagcatct acttcggctt
     1381 cgcttacgca ttgcggtttg gcggcaacga tgtgcatacc gattttgcgt gctacatctt
     1441 cagagccttt gtactcgacc aaaacgcctt cggtcgccaa tgcgccgtgg atgtaggcaa
     1501 ccagttggtt ggcagtgtca atcacttgga agcggcggac agacatgttc tcgcccaatt
     1561 tagcgatgat ggctttgcgt tctgattcaa ccagttcgct cagttcttca acagaagccg
     1621 gttttttctc ggcagcagtt ttcgcaacga agttggcaaa ttctacgaag ccggcgtctt
     1681 tagcaacgaa gtcggtttcg cagtttactt cgaccaatgc gccgacatta ccgttgattg
     1741 cataagccaa tacgccttcg gcagcagtac gaccagccag tttgcccgct ttcgcaccgg
     1801 atttaatacg caggatttct tcggctttgt cgaagttgcc ttcagcttca accaaggctt
     1861 ttttgcattc catcataccc aggccggtag cggcgcgcag gtcggcaacc atttttgcag
     1921 taatttctgc cattttgaat ctcctagatt tttgggaaat acggaaagga ttttccacat
     1981 tcgggaatcg tgtgccgcga acgggcgttc agacggcata tttgagaaaa ggggcataac
     2041 gcccctcttc tgtgtaccga attactcggc agcggtttct tgggcagcgg ctacggtttc
     2101 ttgcagcgct tggtttttgc cttccaaaac tgcgtcggcg atgcctcggc agtacaggcg
     2161 gatggctttg gcggagtcgt cgttgccggg gatgacgtat ttcacgccgt ctgggctgtt
     2221 gttggtatcg actacggcga taacggggat gcccagtttt tcagcttcaa ccagagtacc
     2281 tttttggtag ccggtatcga taacgaaaat cgcgtcgggc aggcctttca tgtttttgat
     2341 accgcccaaa gagcgttcca gtttttcaac gtcgcgttgc atttccagaa tttctttttt
     2401 gctgaaaccg ctttcggcag cattttccaa ggctgcggtt ttttcttcca ggcgtttgat
     2461 ggattgctta acggttttgt agttggtcag cataccgccc aaccagcggt ggtcgacgaa
     2521 aggcataccg gcgcgggtcg cttcttcgcg gatgatgtcg cgtgcctggc gtttggtacc
     2581 tacgaacaat accgtacctt tgttggcaac cagacgacgt acggcttctt gcgcctcttg
     2641 gaacatcggc agggtttttt ccaagtttac gatatggatt ttgttgcgcg cgccgaaaat
     2701 gtattgagcc attttcgggt tccagaaacg ggtttggtga ccgaagtgaa caccggcttc
     2761 aatcatctga cgcatagtaa tttgagacat gttatttcct tgaaagggtt aaagagcaca
     2821 cattcaatcg catagaactt gataggcggc gcctattgtc aagcaccctt cggcgaaagt
     2881 gggcataagt tttaaaaata gtgtgtttcc gaaatggaaa actgacggat tatagcggat
     2941 aagatgccgt ctgaaaaggg attcagggtt ttgtgccgct gtctttttgc cgttgtgcgc
     3001 gctgctgttc ggattctgcc tgttcctgct gccccatcag gcggttgcgg cggcggacgg
     3061 ttacggcgaa gaccgtgaac acggtgggca gaaccaccca aaaaaccaaa taaatcaaag
     3121 cgcgcgcaat actcggctgc gcggcggaat acataacggc aacaaaaaga tagccgatgg
     3181 cgacgatgtg ccacattgtg tgcgtcctgt ttgaatgtta acaaaatcgt tataatcgca
     3241 acattctacc gcaatcctga aaggccgtct gaaatgagga acgaggaaaa acgcgccctg
     3301 cgccgcgaat tgcgcgggcg gcgttcgcaa atggggcggg acgtgcgggc ggcggcggcg
     3361 gtaaaaatca accgcctgct caaacgttat atcaagcgcg gtcggaaaat cggcgtgtat
     3421 tggccgatgg gcaaggaatt gcgtttgggc ggctttgtcc gcgcggcgca aaaacgcggc
     3481 gcaaaactct atctgcctta tatcgaaccg cacacgcggc ggatgtggtt tacgccgtat
     3541 cctgaacgcg gaatggaacg ggaacgcaag cgcggtaggg cgaagctgca tgtccctcag
     3601 tttgcagggc gcaaaatccg cgtgcacggt ttgtcggtat tgctcgtccc gcttgtcggc
     3661 atagaccgcg aaggctaccg tttggggcag gcaggcggct attacgatgc gacgctttcg
     3721 gcgatgaaat accgtttgca ggcgaaaacc gtgggcgtgg gctttgcctg ccagttggtg
     3781 gacaggctcc cacgcgaggc gcacgacctg ccgctggacg gttttgtatc ggaagcgggg
     3841 atattgtgtt tttagccgtt cagacggcat tttgggcgga aatgccgtct gaaggcggaa
     3901 tattgggaga aaaggatgtc ggaaaggata atactgtcgg tcttgtcgtt gggcggcgta
     3961 cggctcgaac cgttggatgt gcatcatgaa acgggtttgc gcgaggcggt ttgcgatggg
     4021 gaggtttgga agctgggggt aacgtccgcg ccccatccgg atagggtggc agactatatc
     4081 gggacagcat tggcaacgcg tttggcgttt gcggttgtcg atgaagaggc ggacagggtg
     4141 gtcgggacaa cggcgtatta tcattttgaa ccgcagattc cgcgtttgga tatcggattc
     4201 acatggtatg cggcatcggc acggcggaaa cggataaaca cctgttgcaa aatcatgctg
     4261 ttggattacg catttgatgt tttagcttgc tgttgcgcgg gatggcgcac ggatattctg
     4321 aaccttgcct cgcaacgtgc catcgaacgg ctgggcgcgg aaaaagacgg cgtgctgcgt
     4381 atgcatatgc tccgtaaaga cggcagtgtg cgcgatacgg ttgtgtacag tatgctgagg
     4441 gaagattggt gcaaaaacag ggaaatcctg accggcaggt tggcagggta tggcgtgcag
     4501 gtgtgaggca gtattctcaa atttaaaaaa cttaacgcga tatgctgaca atcttttaaa
     4561 aattaatata taaacaattt tgattttttg tctattaaaa atttgatata tatagataaa
     4621 taactatttt tattttttgt ttcgcagttc cccggcttgg cgggcgacag aaaaactttg
     4681 catttcccag taaaatccac tacattcacg ataacatatt acaagcccct gaagcggtgt
     4741 cggaagccat acttgcaacg cattccaacc ccgaaacagc gtgctcggac acacaccatt
     4801 gccggtttcc cctgcttttg cgattcaggc gtttgtagta cggcaaataa ccataagggc
     4861 agaagatgcc tgttttttag gagtaaacca tggctgaagc gacagacgtt gtcttggtgg
     4921 gcggcggcat tatgagcgcg actttgggcg ttttgctcaa agaactcgaa ccgtcttggg
     4981 aaatcaccct gattgaacgc ttggaagatg tggcgttgga atcgtcaaac gcgtggaaca
     5041 acgccggcac ggggcattcc gcgctgtgcg aattgaacta tgcgccgctg ggtgcggacg
     5101 gcgtcatcaa tccggcgcgc gccctcaata ttgccgaaca gtttcatgtc agccgccagt
     5161 tttgggcgac gctggtcgcg gaaggcaagt tggaagacaa ttccttcatc aatgccgtgc
     5221 cgcatatgtc tttggtgatg aacgaagacc actgccgtta cctgcaaaaa cgctatgatg
     5281 tgtttaaaac gcagaaactt tttgaaaata tggaattttc caccgatcgg aacaaaattt
     5341 ccgattgggc tccgctgatt atgcgcggcc gggacgaaaa ccaacccgtc gccgccaact
     5401 attccgccga aggcacggat gtcgatttcg gacggctgac gcgccagatg gtgaaatatt
     5461 tgcagggcaa gggcgtaaaa accgagttca accgccacgt cgaagacatc aaacgcgaat
     5521 ccgacggcgc gtgggtgctc aaaaccgccg atacccgcaa cccagactgg cagctcaccc
     5581 tccgcacccg cttcctcttc ctcggcgcgg gcggcggcgc gctgaccctg ctgcaaaaat
     5641 ccggcatccc cgaaggcaaa ggctacggcg gcttacccgt gtccggcctg ttcttccgca
     5701 acagcaaccc cgaaaccgcc gaacaacaca acgccaaagt gtacgggcag gcttccgtcg
     5761 gcgcgccgcc gatgtccgtc ccgcacctcg acacacgcaa cgtagacggc aaacgacacc
     5821 ttatgttcgg tccttacgca ggtttccgtt ccaacttcct caagcaaggc tcgtttatgg
     5881 atttgccgct gtccatccat atggacaacc tctatcctat gctgcgcgcc ggctgggcga
     5941 atatgccgct gaccaaatac ctgctgggcg aattgcgtaa aaccaaagaa gaacgctttg
     6001 cctccctgct ggaatactac cccgaggcaa accccgacga ctgggaactc atcaccgcag
     6061 ggcaacgcgt ccaaatcatt aaaaaagact ccgaaaaagg cggcgtgctg cagtttggta
     6121 cggagattgt cgcccacgcc gacggctcgc tcgccgcatt gctgggagcg tcgccgggcg
     6181 catcgaccgc tgtgccgctg atgatccggc tgatgcacca atgcttcccc gagcgcgccc
     6241 cgtcttggga aggtcgtctg aaagagctgg taccgggtta cggcatcaag ttgaacgaaa
     6301 accctgaaag ggcggatgaa attatcgcct ataccgcgaa agtgttggat atttaaaccg
     6361 aatctttcaa gaaaagccgt acccccatat ggtgttgacg ccgtccctgc cggttatgcc
     6421 cttcatccgg cagggcaggt tttgatacac ggggcaggta atcgtttttt cccttttgcg
     6481 aataatggaa cggtcaaaaa acagcgtttt cagaaatgaa aacgctgttt tttgttgcct
     6541 gattaacgtg gggaacagtc tttgaagacg atttggttgt caggtgcggt aatcataata
     6601 ggctgtttgc ggtaggattt gctgtccatt gcgccggtgc tcagcacata accgccttct
     6661 ttgccgtaga acgtgtccat attgtcggat ttatccaaat tgatgggcat ttgcacacgt
     6721 ttgccgttga tgacggcgga ggcgtatgtg gtcagaccct gtttgttgaa gccgtaggtt
     6781 actttgactt ttttaccttg ctggcagacg tagctgacgg tttttttggc aacggtgggg
     6841 ttgtccgtgc cggcggcggc catactgctg agcgcgattg cggaagacag gattgcagtg
     6901 gtcagaagtt tcatttttat tcctttggat agatgtggag tttgcccgtt tcggactgtt
     6961 tgacagtcta atgtcggcgt atcctgaaat aaacaggaaa ttggataaag tttgtaaaaa
     7021 ggcaggttcg ggcggttcgg cggtttgagg caggggcggc aggcgggata cggcggcctg
     7081 tccggtgttt cagacggcat tgtttgcatt tttcggaaat gaaaaacctc cgggcatccg
     7141 gaggttgcgg tttaaccttg tttttttctt tcggccttgg tctgtttgga caattcggca
     7201 tacaggtcgg gatggtgttt tttcaacagg gcggcgattt ccatatcttt tttgttgact
     7261 tttgccaaat cgaggcattc gatatggacg aggcggagca gttcgcgtac gggtttgggc
     7321 atcttgcggc cggtttcgta gcgtgaacct ccggattgag taacgccgac cttgccccag
     7381 aagtctatct ggttcatccc ggtcttttta cggatggctt taacatcttc tatattttca
     7441 aatattttca taaaacttcc tttaagtttt tctggttgcg gggcaaattc cgatttgctt
     7501 tgttttcgta atgtgccggg tcaagcctgt ttttatattg catacagctt cagggtttgc
     7561 cggtggcggg gctgacggtg aggatttcgt agccggtttc ggttaccaag acttcgtgtt
     7621 cccattgggc ggagagggag cggtctttgg taaccaccgt ccagccgtcg ttgaggatac
     7681 gcaggtggcg tttgccttgg ttgatcatcg gttcgacggt aaaaatcata cccggtttta
     7741 gaacgggacc ctgccctttt tttccgtagt gcaacacttg cggggcttcg tggaaaccgc
     7801 gcccgatgcc gtgtccgcag aattcttgta caaccgaata acctgcattt tcggcaacct
     7861 gctggcaggc gtaacctacg ccgcccagcg tcgcgccagg tttgacggct tctatgcccg
     7921 ccatcataga ggcgtgggtt acgtcgatca ggcgttgggc gatgggggag actttgccga
     7981 cggtaaacat acggctggag tcgccgtgga aaccgtcttt tttgatggtg aggtcgatgt
     8041 tgataatgtc accttctttg agcggcttgt cgtcgggaat gccgtggcag atgacgtggt
     8101 tgacggaggt gcagcaggat ttggggtagg gcgggttgcc gtagtggagg ggggcgggat
     8161 agccgccttg gacgttgacg tggtagtcgt aaacaagttt gtcgatttcg tcggtggtga
     8221 tgccgggttt gacgaattgt ccgatgtagt cgagggcttc ggcgacgagt ttgcccagct
     8281 cgcgcatttt ttcgatttct tcgggggttt tgatgatgat gccgttcata atgcttcctt
     8341 ggattgcggg tgtgcctgcc gtgccgggtc aaaagaggtt gtcgagaatc tctttatggg
     8401 gtttggccgg cgtgtttttc ggggtgtccg gtttggtatg gttttctttg ggcgtttctt
     8461 tttctttggg tgtttctttg ggctgcgcct gtttttcggg tgtttctttt cggggttcgg
     8521 gatgggggac gtttgtgggc agtacggggg tttctttgag tgtgttttca gcttgtgttc
     8581 cggtgccggt taacgaatcg gctgcccggg ccgcatccga agggcgcggt ttgacccggt
     8641 ttttcttggg tgcggcaggg ggcggcagtc cgctgccgct ttcttctgtg ccgtccggct
     8701 gtgcgggttc ggatgaggcg gcgtggtggt gttcgggttg ggcggcatct tgtccgacgg
     8761 cgccgtttgg cagccagaat tcggtttcgc gggggctgtc ggtgtgttgc ggttcggttt
     8821 ggagggtttt gtcgccgttc gggttgaatg tgctgacgat gcctactgcc aatgcgccga
     8881 tggcggtgat acagatgagc aatacggcgc gtatcaggag tttgggggtc agcctgaagg
     8941 gtttgttcgg tttttttgcc attttgattg tgcttttggg gtgtcgggca atgccgtctg
     9001 aaggcggttc agacggcatt gccgagtcag cgttggacgt agttttggta gagggtaatg
     9061 actttttgta cgccgacggt ggtgctgact ttttgggtaa tctgcgcctg ttcttcgggg
     9121 gtgaggatgc ccataacgta ggttacattg ccgtaggtaa tgattttgac gcgcgcctgt
     9181 gtagcggggc tgatgcccag cagcgtggcg cggactttgg acgtgttcca agtgtcgccg
     9241 gcgatgtcgc ccgcagtgcg cggcagggag gcgacggtaa tgtagttgta tacgccttcg
     9301 gcggcctgtt cggaacgtgc aatctgaccg acgaactgtt tttcgccttc ggtggcgact
     9361 tgtccgagca gcagcaggtg gcggttgtag ccgacgacgg agatttgggg cgtgtagcct
     9421 ttggtttggt tgttttggcg caggtaggaa cgggcggtgg tttcgatacg caacgccata
     9481 acgttgtcat cggtttgcgc gccggtggtt cggcggtcga tgacggattt cgcgccgacg
     9541 gcggccccgc cgacgactgc gctgaagcag ccgccgaggg caaggctgag gacggcggca
     9601 atcagggtgc ggacggtgtg tggtttgggt ttcatcgggg acttcctttc ttgggcgttt
     9661 cagacggcat tgctttgcgc catgccgtct gaaaagggtt acattccttc cagcagtacg
     9721 gagtcgatac agtcgcatat ggcgtgtatc agcaggatgt ggttttcctg aatgcgggcg
     9781 gtgcgcggat gggggacgtt gagcagaacg tcggtgtctt tgagcatggc ggcgattttg
     9841 ccgccgtcgc ggccggtcag ggcgatgacg tgcatatcgc gttcgtgcgc ggctttgacg
     9901 gcttcgatga cgttggcgga attgccggag gtggaaatgc cgacgaggac gtcgcctgca
     9961 cgtccgagcg cgcgcacttg tttgctgaat acgtggtcga aaccgtagtc gttgccgatg
    10021 gcggtcagcg cggaggtgtc tgtggtcagg gcgacggcgg cgagttccat gcgttctttc
    10081 tcaaaacgcc cggtcatttc ggcggcgaag tgttgcgcgt cggcagccga accgccgttg
    10141 ccgcaggcca ggattttgcc gtcgttcatc aggcattgca gcatcagctc ggcagcctgt
    10201 acggtcggct cgaccagtat tttttcggct tcctgtttgg cacggatgct ttcggcaaaa
    10261 tgtgcggcaa cgcgttcttg taatgtcgtc atatcgtcaa cctgtaatat tctttatcca
    10321 ctcgggcggg cggtttcctt cgataagtac cgcatcgagg cggcacggta cgtttgtcag
    10381 cctgttctgt tgcagataat actctacact tcgttgcagt ttcaataatt tggacggaga
    10441 aatgctgtat gccgcgccgc cgaaccgttg gtttttgcgg tattttactt caacaaacag
    10501 aatcatgccg ccgtttttga caatcaggtc gatttcgccg taggcgcagt gccagttgcg
    10561 ggcaagcagc gtgcagcctt gggattggag gaaggcaagt gcggcatctt cccctgccgt
    10621 accctgtttg tggtttaagc gcataatcgg tttaaccttt ataatgtctg tttttcagac
    10681 ggcatttttt atgtttcaga aacacttgca gaaagccccc gacagcgtcg tcggagggac
    10741 attatacgtg gttgccacgc ccatcggcaa tttggcagac attaccctgc gcgctttggc
    10801 ggtattgcaa aaggcggaca tcatttgtgc cgaagacacg cgcgttactg cgcagctttt
    10861 gagcgcgtac ggcattcagg gcaggttggt cagtgtgcgc gaacacaacg agcggcagat
    10921 ggcggacaag gtaatcggtt tcctttcaga cggcctggtt gtggcgcagg tttccgatgc
    10981 gggtacgccg gccgtgtgcg acccgggcgc gaaactcgcc cgccgcgtgc gcgaagcagg
    11041 gttcaaagtc gttcccgtcg tgggcgcaag cgcggtaatg gcggcgttga gtgtggccgg
    11101 tgtggcggaa tccgattttt atttcaacgg ttttgtaccg ccgaaatcgg gcgaacgtag
    11161 gaaattgttt gccaaatggg tgcgggcggc atttcctgtc gtcatgtttg aaacgccgca
    11221 ccgaatcggg gcaacgcttg ccgatatggc ggaattgttc cccgaacgcc gtctgatgct
    11281 ggcgcgcgaa atcacgaaaa cgtttgaaac gttcttaagc ggcacggttg gggaaattca
    11341 gacggcattg gcggcggacg gcaaccaatc gcgcggcgag atggtgttgg tgctttatcc
    11401 ggcgcaggat gaaaaacacg aaggcttgtc cgagtctgcg caaaatgcga tgaaaatcct
    11461 tgcggccgag ctgccgacca agcaggcggc ggagcttgcc gccaagatta caggtgaggg
    11521 caaaaaggct ttgtacgatt tggcactgtc gtggaaaaac aaatgatgcc ggatggaccg
    11581 gcaccatagt gcgaacggaa tcaacggaca cgccccgtca tgcggtacag cacgtctttg
    11641 ccttgaacac ggtggacgac gaccatggcg acgtgtccga cgacggcggc aaacagcagc
    11701 cagcccaaat tgccgtggaa cgtgttgccc aagtttgcca tccactcgat tttttccggc
    11761 gaaccctgca ttacttcaac gccgaacact ttcaacgggc cgcgtccgcc accgtattgg
    11821 cggatcatgc cgataacggg aacggcaagc atgagcagat acagaatgcc gtgtcctgcc
    11881 gccgcagcct tgctgtcgct ttgcggacgc ttggcgcggt tggcaaccgc ccacacgatg
    11941 cgcaatgcaa tcaccgtcag catgaggaaa ccgaaagatt tgtgcaggcc gaacaggctg
    12001 cccgcccatt ccgcctcttc gtaaattgtc cacaggacaa tggtggtcag aatgccgatg
    12061 aagccggcaa cggtcagcca gtgcagcgcg cgtgtcaacg ggtcgtaaac ggcaggttgg
    12121 tttgtgttca taaaaaaatc cttatcatat gtaacggttt gaacggacat tccgtcgggt
    12181 cggaatgtca aaaaggcgcg attgtaccaa agaagttgga catattttgt ttgcaggctg
    12241 aagatttgct taaaaattca ttaagatggg cagaacaaat agtttctccg cggtatattg
    12301 aaataccgct tataccttat aatgtcggca gaaaatctgt tcagacggca tcaaaacata
    12361 tggcaatccg cctgaaaccc tatttaaaac caacaaacaa gagcatttaa tgaaattcat
    12421 cgacgaagca aaaatcgaag tcgccgcagg caaaggcggt aatggcgcaa ccagtttccg
    12481 ccgcgaaaaa ttcgtacccc gcggcggccc ggacggtggc gacggcggca aaggcggcag
    12541 cgtctgggca gaagccgacg aaaacaccaa caccctcgtc gaataccgct tcgtcaaacg
    12601 ctaccaagcc aaaaacggcg aaaaaggcca cggttccgac cgctacggcg caggtgcgga
    12661 cgacattgtc ctcaaaatgc ccgtcggcac ccttatccgc gacctcgaca ccgacgaaat
    12721 cgttgccgac ctcacctacc acggacagcg cgtctgcctc gccaaaggcg gcaaaggcgg
    12781 cttgggcaac atccacttca aatcctccgt caaccgcgcc ccaaaacaat ccacgcccgg
    12841 cgaagaaggc gaaacccgtt ccctgcaact cgaactcaaa gtcctcgccg atgtcggctt
    12901 gttaggcatg cccaacgccg gcaaatccac cctgattacc gccgtctccg ccgcacgccc
    12961 caaaatcgcc aactacccct tcaccaccct gcatccgaac ttgggcgtgg tacgcatcga
    13021 cgaaaaccac agcttcgtga tggccgacat ccccggcctg attgaaggcg cggcagaagg
    13081 cgcaggcctc ggccaccgct tcctcaaaca cctatcacgc accggtctgc tgttacacgt
    13141 tgtcgatttg gcacccttcg acgaaaccgt caatcccgcc gaagaagcac tcgccatcat
    13201 caacgaatta cgcaaatacg acgaagaact ttacggcaaa ccgcgctggc tggtgctgaa
    13261 caaactcgat atgctcgacg aagaagaagc ccgggcgcga acagccgcct tcctcgaagc
    13321 cgtcggctgg gactaccccg aacctgatga ccgtttccaa ttcgacatgg aaaccccgcg
    13381 cctcttccaa atcagcgcgc tgacacatca aggcacgcag gagttggtac accaaatcaa
    13441 ccaatacctg gccgagaaaa aacgtatcga ggctgaaaaa gccgaagcgg agaaagcagc
    13501 ggcaaatgtt gaaattatcg aacagcagcc taaaacggat actggcgtgt ttaagccgga
    13561 gtaaaaggac aaggccgtct gaaagttttc aggcggctca ttctgcagag atgtgctatg
    13621 tacaaaacaa tagatttatt ttcaggcatt ggcggaatac gcctaggctt tgaaaaatac
    13681 gggtgcacca atgtattctc atcggaatgg gacaaatatg cccgacaagt gtatgaagca
    13741 aactttggag aaaaaccttt tggcgacatt aacggcatcg atccttcaga tattcccgat
    13801 catgatattc tgcttgcggg atttccgtgc caacctttca gtatcgcagg taagggattg
    13861 ggatttgaag atacgcgcgg aacattgttt ttcaatattg ccgaaatcct gaaaaccaaa
    13921 cagccgaagg cttttcttct ggaaaacgtt aagcggctga ctacgcacga ttcgggcagg
    13981 actttccgta ttattttaga aacccttaaa cagcttggtt atacggttta ttttaaggtt
    14041 ttaaatactt tggatttcgg attgccgcaa aaacgcgaga gaatttatat tgtgggtttt
    14101 tcagacaaca tcccttttta ctttcccgaa cccataaacc aatatcggcc attgggcgaa
    14161 ctgctggaaa acgatcgaga tgtcgaacca agctattttt tatcagatac cttgaaacaa
    14221 aaacgtttgg cagccttaaa aaaagctccg cccaccccat caatttggca tgaaaatatt
    14281 ggcgggaatg tttccgcact tccatattcc tgtgccttaa gggccggggg aagttacaat
    14341 tatttggtgg taaacggagt aagaaggcta acaggaaggg aaatgttaag attacaaggt
    14401 tttcccgatg attttgaaat caatatcccc tactcgcaag tcaggaaagt cgccggaaat
    14461 tctgtatccg tccctgtaat agaagccatc gcggaaaata tgctcgcttc tctttccggc
    14521 aaggtcgaac aaaaagggca attggattta ttggaggcag gatgacgctt gaagaacaac
    14581 aagccaaaga agcattggac ggcattatca aaaaatcccg tgtccacctt tataaaccca
    14641 ttcaaattgc agaaatttta tatcatgacc gttgtataaa acagttggat tttttaaatt
    14701 tagatactta ccgcaaccaa tctaaacgct ggagggatga aatctgccgt cggtttttag
    14761 ggcggatttc tacttcatct gcaaaattcc aagataatct gtttgaaaaa aatgcaatac
    14821 cgcctgaaaa actagcggta ttgggaacat taaacagaca gtcggacggc gggggagaat
    14881 cgtacattta taaacagttt ttcaatcgtt tttcccaaat gagcgaagca ttggcttatg
    14941 tcggcaatac agacagatac tccttccaac tgtctgaatt tctaaattta ttttggctcg
    15001 agccgggatt gaaaagaagt atagacaaga tatatgaaat cgttgtttat gcgttgtttg
    15061 atgcattggt ttcagaatta ggcataacgg tttcaattga ttttcctaag gaaaacctgt
    15121 ttttatggga ggaatatcaa gattttgccg aaaaaatcat cactatgccg aaaaatgagc
    15181 atttaaaact tcctgcaaaa atccatcgtg taggcgtaac caatgctgcc gatagagggt
    15241 tggatatgtg gtctaacttc ggattggcca tacaggtcaa acatctctct ttagacgaag
    15301 agttggcaga agatattgta tcctccatta gcgcagatag aattgtcatt gtctgcaaaa
    15361 aggcagaaca atcggtgatt gtttcattac tgacgcaaat aggctggaaa agccgaattc
    15421 aaaacatcgt aactgaggat gatttgataa gttggtacga aaaagcattg agaggccaat
    15481 atccgattgc agaagcgttg ttggaaaata ttaaaactga aattatgcgt gaatttcctg
    15541 ccgttaatga agccaatgaa tttttagatt tcgcccaaaa tcgcggatat gacattactg
    15601 ttactcattt ctaaacttac ttgatgaata tgaccgccat ctacaacacc cttacccgtc
    15661 aaaaagaacc cttcgccccc atcgaccctg aaaacgtgcg tatgtacgtc tgcggcatga
    15721 ccgtttacga ctattgccac ttaggccatg cccgtgtgat ggtggtgttc gacatgattg
    15781 cccgctggtt gcgcgagtgc ggttatccgc tcacttatgt gcgcaacatc accgacatcg
    15841 acgacaaaat catcgcccgt gcggctgaaa acggcgaaac catcggcgaa ctgaacgcgc
    15901 gtttcattca ggcgatgcac gaagatgccg atgctttggg cgtgttgcgt cccgacatcg
    15961 agccgaaggc gacggaaaac atcccgcaaa tgattgccat gattgagacc ctgattcaaa
    16021 acggcaaggc atatcctgcc gcaaacggcg acgtttacta cgccgtgcgc gagtttgccg
    16081 cttacggaca attatcgggc aaatcgttgg acgacctgcg tgcgggcgaa cgcgtggaag
    16141 tggacggttt caaacgcgat ccgcttgatt ttgtattgtg gaaagcggcg aaagcaggcg
    16201 aaccggcgtg ggaaagcccg tggggcaacg gccgtccggg ttggcacatc gaatgttccg
    16261 cgatgagtga aaacctgttc ggcgatacgt tcgacattca cggcggcggc gcggatttgc
    16321 agttcccgca ccatgaaaat gaaatcgccc aaagcgtcgg tgccagcggt cacacctgcg
    16381 gtcatgacca cgcgcaaacc caccacggtc aaagtattgc cagccacgtc aaatactggc
    16441 tgcacaacgg cttcatccgt gtggacggcg aaaaaatgtc caaatcgctg ggcaacttct
    16501 ttactatccg cgaagtattg aaacaatacg accccgaagt cgtgcgtttc ttcatcctgc
    16561 gcgcccacta ccgcagcccg ttgaactact ccgacgcgca tttggacgac gcgaaaggcg
    16621 cgttgacccg tctgtacacc accttgaaaa acacaccggc ggctgagttt gatttgtctg
    16681 aaaacgccaa cggctacacc cgccgtttct acgccgccat gaacgacgat ttcggtacgg
    16741 tcgaagcggt tgccgtgttg tttgagctgg caggcgaagt gaacaaaacc aatgacgcac
    16801 acctcgccgg ctgcctgaaa gctttgggcg gcatcatcgg cctgctgcaa cgcaacccga
    16861 ttgagttcct ccaaggcggc gcagtttcag acggcctctc caacgaagaa atcgaagact
    16921 tgattgcccg acgcaaacag gctcgcgcgg ataaaaactg ggcggagtcc gaccgcatcc
    16981 gcgaccttct gaacgaacac aaaatcattt tggaagacag cgccggcggc acgacttggc
    17041 ggcgcggttg aactgtatcg gaaagggcaa aagtaccgcc ttttgccctt ttttcgtttt
    17101 cagacggcat ttcccgcttg aagccccgcc gttttcagca ggcaggaccg aatgggaaat
    17161 accgggcggc gcaccgggtg ccgctttgtg tatgggaaca ttgtgagaac ttatttttat
    17221 tatgcttaaa atcatttccg ccaacgtcaa cggcatccgc tccgcctaca aaaaaggttt
    17281 ttacgaatac atcgccgcat cgggcgcaga cattgtctgc gtgcaggaac tcaaagcgca
    17341 ggaggctgat ttgtctgccg atatgaaaaa tccgcacggg atgcacggcc attggcattg
    17401 cgccgagaag cgcggttaca gcggtgtggc ggtgtacagc aaacgcaaac ccgacaatgt
    17461 gcaaatcggc atgggcattg aggaattcga ccgcgaaggg cggtttgtgc gttgcgattt
    17521 cggcaggttg ggcgtcattt cgctttattt gcccagcggc agcagcgcgg aagaacgcca
    17581 gcaggtgaaa taccgtttcc tcgatgcgtt ttaccctatg ctcgaagcga tgaaaaacga
    17641 agggcgcgac atcgtcgtct gcggcgactg gaacatcgcc caccaaaaca tcgacctgaa
    17701 aaattggaaa ggcaatcaga aaaattcggg cttcctgccc gaagagcgcg agtggatagg
    17761 caaagtcatc catacgctgg gctggacgga catgtggcgc acgctctatc ccgatgtgcc
    17821 gggttataca tggtggagca accgcgggca ggcgtatgcg aaagatgtcg ggtggcgcat
    17881 cgattatcag atggttacgc ccgaacttgc cgccaaagcc gtgtccgcac acgtttataa
    17941 agatgaaaaa ttctcggatc acgccccttt agttgtggag tatgactatg ctgccgaata
    18001 aggttttggg taaatatgat tggaatgtgg acggaaaaac aggaataggc gcggcatggg
    18061 ttgtcgctgc gtttatcctg cccatattgg tgtgggcggt gtttatgttg tcgcggatgc
    18121 aaggctggct tgcgccgacc aaggcaaacc cgatatgggc gttggtgtgg ctgctgattt
    18181 gtctgccctg cctgctgatt gcggccaaat gtttgggttg gaaaggctgg cggcgggtgg
    18241 tgaatatttt tgtctgcctg accgtctgcg ccatattgag cgtacccgct tcgctgctca
    18301 tcgcctttac cctccgggat ctgctcaaat agtcgcggac aatggaaagt ttcgagcttg
    18361 aggatttgac gctgtggctg atacgcgatg cggatgaagc ggaaatgtgg atagaccgtt
    18421 gggcggtcag ttatcccgtt gtgcagatgt ccgaagcgtc cgcaaacggc agcacggaag
    18481 cgtggcaaag cgcgcttcag acggtattcg aacggataca gggcaggcat atcgccgttg
    18541 tcgcacacgg cgcgggcgcg gccgcattct tggcgtggct gtaccgcgcc gacatcctga
    18601 cgcagaagaa actcgccggt atcattctcg tatctccgcg tcccgatatt tttcccgacg
    18661 atgcggaaca cactttccaa cgcgtccgct gtccttgccg tgccgcattg gtggtgtccg
    18721 aacacggcgg cgtgccgcac ggttgggcgc aaaaacaggc ggatttgtgg aacgcccgcc
    18781 tgttggtttc cccgcattcg ggcagtttga acggtatgct cggcggctgg cagtggggta
    18841 tgaagctgat gcaggaaatg ttgctggcgt gaacggtcgc tttatcggaa tttcggttaa
    18901 ccggggttgc aaggaaaata ccgtctgaaa caggcttcag acggcatttc cattcaaaca
    18961 gggcttacag gctgcccaac acgatacgca acacgcggcg catcggttcg gctgcacccc
    19021 acaaaagttg gtcgccgacg gtgaacgcgc tgatgtattc gccgcccatg cccagtttgc
    19081 ggatgcgtcc gacagggacg gacagcgtgc cggtaacttt ggcgggcgtc agctcgcgga
    19141 tgccggcttc tttttcgttg gggacgactt tcacccagtc gttcgcgcct gccaaaatcg
    19201 cttcgatttc ggaaacaggc aggtcttttt tcaacttcag agtgatggct tggctgtggc
    19261 agcgcatcgc gccgatgcgg acgcacaggc cgtcgatgac ggtcggattg ccgctgcgtc
    19321 cgaggatttt gttggtttcc acgccgccct tccattcttc tttggactgg ccgttgccca
    19381 aatccacgtc aatccacgga atcaggctgc cggcgagcgg tacgccgaag ttggctttcg
    19441 gatagtcttc gctgcggagg aaatcggaca ctttgcggtc gatatcgaga atcgcgctgg
    19501 aaggatcggc aagctcgtcc gccacttggg cgtgaatcgc gcccatgccg ctgatgagtt
    19561 cgcgcatatt tttcgcgccc gcgccggaag cggcttggta ggtcatgctg gtcgcccatt
    19621 cgaccaaatc gttttggaac aggccgccca aagccatcag catcagggag acggtacagt
    19681 tgccgccgat gtagtttttc acgccgtttt tgagaccgtt gtcgatgacg ttgcggttga
    19741 cggggtcgag ggcgataatc gcgtcgtctt tcatgcgcag tgaggacgcc gcgtcaatcc
    19801 agtagccgtt ccagccgctg tcgcgcaggg cttggaagac ggatttggtg taatcgccgc
    19861 cttggcaggt gacgataatg tccattttcg ccagctcggc aacgtcgttc gcgtccaata
    19921 atgttttagc cgcctgaccg aaatcaggtg ccgcgccgcc gacgttggaa gaggtaaaga
    19981 aaaacgcttc gggaatgtgg gcgaagtcgt tttcttcttt catacgctgc atcaaaaccg
    20041 aaccgaccat accgcgccag ccgacgaaac ctactttcat gtattgctcc ttgaggaaaa
    20101 tcgttaataa aattatgtat ggcgtaaaga aagcgtttta acgccgtatt gggaaaatgt
    20161 aaagttcttt atcgaatttt ctttttggga ttggcgtttg cagcagtagg gcggtagaat
    20221 tccatctgtt taagaacaat tatcaggaac ggatatggaa gaaaaaaacg actacaccga
    20281 cgcggtatcc gacaaccgca acgggcagga aattgaagtc ggaattgccg gcgcaggcga
    20341 ccggatactt gccgcgctgc tgaaccaatt gtttaccttt ttgattttgt tggtgccgtt
    20401 tgtcggactg attgccttcg ccgtcaaaaa tgaaggcagg attggcggcc gtgaggaaat
    20461 attcgggctg cttttgggca tgacttcctt ttgggtcgga ctggcgggca ttttggcgta
    20521 caccgtcatc caaatctact atatgagcag ggacgggcag tcattgggta agaaaatcat
    20581 gagaatccgc gtgttgaaaa ccgacggccg caatccgggt tttgtcggca cggttttggt
    20641 acgcgaaatc gcgtggtcgg ttttggttgc cattattgcc gccgttatcg gtcttgcagt
    20701 aggtgaaaac ggggaaaacg ccatcaacct gctggcattc cttgccaact ttgtcctgct
    20761 ctttatggtc aaacgcgacc gccgcacgct ttacgacata ttggcggata cggttgtcgt
    20821 caagctgccc aaataaacgg acgcggcaaa atgccgtctg aaaggctttc agacggcatt
    20881 tttatcttca gaaccgctca agcatcgtgc agcgaagctg cgtgcaaagt gttttccatt
    20941 aatgtggcaa tcgtcatcgg acccacgccg ccgggaacgg gcgtaatcat cgccgcccgt
    21001 tcttttgccg tttcaaattc cacgtcgccg cacaggctgc cgtcgtccaa acggttgatg
    21061 cccacatcga taacgaccgc gccgggtttg atccacccgc ctttgacaaa gttcggaatg
    21121 cccacgccga ccaccaaaat atcggcggcg gcaacttcat cggcaagatt ttcggtcgca
    21181 ctgtggcaga ccgttaccgt tgcgcgcgcc agcagcaatt ccaacgcctg cgggcggccg
    21241 acaatattcg acgcgccgac cacgaccgct tttttcccct tcggatcaat gccgtaagcc
    21301 tccaaaagcg tcatcacgcc cttgggcgta cacgggcgca tcagcggcat tttgaccgcc
    21361 agcctgccga cattgtaagg atggaagccg tccacgtcct tatccggcga aatacgttcc
    21421 aagatcgctt ggctgtcgag gtgcttcggc agtggaagct gaaccagaat accgtccact
    21481 tcggaatcgg cattcagacg gtcgaccaat gccagcagtt cttcctgcga tgttgattcg
    21541 ggcagttcgt acgacagcga cttgatgccg catttttggc aggcggtttt cttgttgcgg
    21601 acataaactg cgccggcagg gtcgcctccg accaaaacca cggccaggca aggcgtgtgc
    21661 agattgtcct gttggcgttg cgccaccgct tcggcaatcg cctgcaggtg tttttgcgaa
    21721 acttctttac cgttgatcag ttgtgccgac atatccgtcc ttttgttaaa caggtagaaa
    21781 atgctgaaaa ttatcgcatt ttccgtttgt ttttacagca gaatgccgaa aataaatttg
    21841 ccccgaaata aaatacggct gtttggacgg attaaatatt ttttcaaatc aaaaaactat
    21901 atttttcatc ttggtgtttt gaaaataaat gttgacgcat ctttccctga actgtatatt
    21961 ccgcttcttc aagtcggggc gtagcgcagc ccggttagcg catctgcttt gggagcagag
    22021 ggtcgtgagt tcgaatccca ccgccccgac caatcaatca gcacgaatcg gcatgagcgc
    22081 ccgtagctca accggataga gcaccgacct tctaagtcgg gggttacagg ttcgattcct
    22141 gtcgggcgtg ccaagaattt gattgaaaac cgtttttatg gtggctgtag ctcagttggt
    22201 agagccccgg attgtgattc cggttgtcgt gggttcgagc cccatcagcc accccagata
    22261 aaggcccgta cttttcggta cgggtctttt cctgtttgcg cgtactgtcc gcgccgccgg
    22321 ctttgccttt cgcatatgct tctgtatcat gtgggttttt ctgtctttta tggtttttgt
    22381 cggaggcttt gatggttgac gcttggtctg tccctgattt tgaatcgaaa atctgtccgc
    22441 ccgaggcatt ggcggcgcgt ttggcgttgt tgccgcgccc gctggtgttt accaacggct
    22501 gtttcgacat cctccacagg gggcacgtta cttatctggc gcaggcgcgt tcggcagggg
    22561 cggcgttggt gctggcgttg aataccgatg cttcggtgcg gcgtttgggc aagggcggcg
    22621 accgcccggt taatcctttg gagaaccgtg ccgccgttgc cgccgcgttg gaaagtgtgg
    22681 atttggttac ttggtttgac gaggatacgc cggcggcgtt gattgaggcg gtcaagccgg
    22741 aggtgttggt caagggcggc gattgggtcg tggataagat tgtcggtgcg gcggaaacgt
    22801 tggcacgcgg cggtcaggtg ttttcaattc cgtttctgca ccagacttcg acaacgaaga
    22861 ctttggcaaa aatccgtgcg gcagagggcg gaaaatgacg gttttgaagc cttcgcactg
    22921 gcgggtgttg gcggagcttg ccgacggttt gccgcaacac gtctcgcaac tggcgcgtat
    22981 ggcggacatg aagccgcagc agctcaacgg tttttggcag cagatgccgg cgcatatacg
    23041 cgggctgttg cgccaacacg acggctattg gcggctggtg cgccccttgg cggttttcga
    23101 tgccgaaggt ttgcgcgagc tgggggaaag gtcgggtttt cagacggcat tgaagcacga
    23161 gtgcgcgtcc agcaacgacg agatactgga attggcgcgg attgcgccgg acaaggcgca
    23221 caaaaccata tgcgtgaccc acctgcaaag taagggcagg gggcggcagg ggcggaagtg
    23281 gtcgcaccgt ttgggcgagt gcctgatgtt cagtttcggc tgggcgtttg accggccgca
    23341 gtatgagttg ggttcgctgt cgcctgttgc ggcacttgcg tgccggcgcg ctttggggtg
    23401 tttgggtttg gaaacgcaaa tcaagtggcc aaacgatttg gtcgtcggac gcgacaaatt
    23461 gggcggcatt ctgattgaaa cagtcagggc gggcggtaaa acggttgccg tggtcggtat
    23521 cggcatcaat ttcgtgctgc ccaaggaagt ggaaaacgcc gcttccgtgc agtcgctgtt
    23581 tcagacggca tcgcggcggg gcaatgccga tgccgccgta ttgctggaaa cattgcttgc
    23641 ggaactgggc gcggtgttgg aacaatatgc ggaagaaggg ttcgcgccat ttttaaatga
    23701 gtatgaaacg gccaaccgcg accacggcaa ggcggtattg ctgttgcgcg acggcgaaac
    23761 cgtgtgcgaa ggcacggtta aaggcgtgga cggacgaggc gttctgcact tggaaacggc
    23821 agagggcgaa cagacggtcg tcagcggcga aatcagcctg cggcccgacg acagaccggt
    23881 ttccgtgccg aagcggcggg attcggaacg ttttttgctg ttggacggcg ggaacagccg
    23941 gctcaagtgg gcgtgggtgg aaaacggcac gttcgcaacc gtcggcagcg cgccgtaccg
    24001 cgatttgtcg cctttgggcg cggagtgggc ggaaaaggcg gatggaaatg tccgcatcgt
    24061 cggttgcgcc gtgtgcggag aatccaaaaa ggcacaagtg aaggaacagc tcgcccgaaa
    24121 aatcgagtgg ctgccgtctt ccgcacaggc tttgggcata cgcaaccact accgccaccc
    24181 cgaagaacac ggttccgacc gttggttcaa cgccttgggc agccgccgct tcagccgcaa
    24241 cgcctgcgtc gtcgtcagtt gcggcacggc ggtaacggtt gacgcgctca ccgatgacgg
    24301 acattatctc ggcggaacca tcatgcccgg cttccacctg atgaaagaat cgctcgccgt
    24361 ccgaaccgcc aacctcaacc gccccgccgg caaacgttac cctttcccga ccacaacggg
    24421 caacgccgtc gcaagcggca tgatggacgc ggtttgcggc tcggttatga tgatgcacgg
    24481 gcgtctgaaa gaaaaaaccg gggcgggcaa gcctgtcgat gtcatcatta ccggcggcgg
    24541 cgcggcgaaa gtcgccgaag ccctgccgcc tgcatttttg gcggaaaata ccgtgcgcgt
    24601 ggcggacaac ctcgtcatcc acgggctgct gaacctgatt gccgccgaag gcggggaatc
    24661 ggaacacgct taaaaaatat gccttttttc aacacggggc ggcatccgtc cgaatctgtc
    24721 cggattctgc cgcccgacag actgccgccc gggcgggcgg ggtttcaaac attgaggaaa
    24781 ttctaatgaa atggctcttc acccttttgg cggtactcaa tattgccgta ttcggcggca
    24841 cggtgggcta taaattggcc gttaaagcgg cgggcggcgt gccggaaaac cgggccgtcg
    24901 aaaacacgcc ccccgcaacg cccgccgccg gcaatgcggc ggcatccgtg gaggatacgg
    24961 cggcactgct caaaccgggc gacatcctga gcgaagaaca ggcggagcag ttgcgcttga
    25021 aaaaagaagc ggaacagaaa aaactgaggg agaaaaaaca gcgtgaagaa aaagcccgcc
    25081 gcgaaaaact cgccgccgaa aaggcgcagg cggaacgtga aaacggcgcg gcggatgcct
    25141 tatgcgccgc gcaggcaagc ctcacgatgg acgaagacga ctaccaccgc atcaaagggc
    25201 ttttgggcaa atggtcgcac gttgccagca ggagcgtcga aaaacgcacc gccaaagcca
    25261 aacctgccga caaaacctac cgcgtcgtcc tgcccgtttc cgccgatgcc gaaaatcagg
    25321 cggcggagct gtctgccaaa ggtttcaacc ccataccgtt tgacggcgca ttgagtttgg
    25381 gtgtcggcaa cagccgggaa aacgcccaag ccctgcaaaa ccggcttgcc gatgccggat
    25441 tcggcggggc gcatattgtc gaacactttg ccgaagccga caggcaggac gattctttga
    25501 gcgtgtcgcg tatgacggtt ttgtttacgg gcgtgaatgc cgccgatgcg gacgaaatcc
    25561 gtaaaatcac gtccctatac ggcaaactga acctcaagtc ttgcaaataa gtgcagacca
    25621 aaaacgccaa acccaccgct agggtgggtt tggcgtttcg gttttgagta tctgattaaa
    25681 aagggaatgt atgtctcgta tatgccgact ccaagtgtga aagtgaattg tgagggaata
    25741 cagtgtttaa tggttcggaa cggacggctt ccgagtggtt ttgattatac gctaataata
    25801 ataattatca ataaaaattt aaaaataaat gcggattttt tgtaatgcga tgaaaaataa
    25861 acaaattaac ctgtccgccg atgtttgtga tttgggtcgg caaactttca ttgcgccgga
    25921 caaatcgggg tcggcaggca tggacggggg cttcgtgcct gtcggaaaga agcctgccgt
    25981 gtttgaacaa ccgtccgatt tggtgcggac agggttgcca ttcgggggtg tttgagcgcg
    26041 gattcgggga tgcaaaagcg gtgaaaccgt tgcggatgaa ggcttacgga tgccccgagg
    26101 tatttttaaa tgtattcaaa cgacaaaaca cccttctatt gccgtactta gggcttgcgc
    26161 agcagcctgc gcgccgggga gtccgggtgt ttcgttggtt gcggtgtcgg ggcgggcggc
    26221 aaggttttcc ggccggtttc cctgttcctc acgacaaagc cgtgatgccg gggaatggcg
    26281 gtttgcccgt ttggggccgc gatgccgtct gaagccttca gacggcattt ttgccgcctt
    26341 tttcaatatt ccgccgaatg ccaaaacggt tgcccgactg tcggcgtgct ggcttgggct
    26401 ttggttcgcg cttcgaccgg cccggcttca aatgccagcc gtccggattc gacggcgagt
    26461 gcgaaggcgc gcgccatgtt gacggggtcg ccgctgcggg aaacggcggt gtttaacaat
    26521 acgccgtcaa aaccccattc catcacttgt gccgcttggg aaggcaaacc caagcccgcg
    26581 tcgataatca gcggcgtgtc gggcaggcgt tcgcgcagga ttttgagcgc ataggcgtga
    26641 accgccccca aacccgtgcc gatgggagcc gcccacggca tcaacgcctg acagcccgca
    26701 tcgagcaggc ggcggcaggc aatcaggtct tcggtgcaat aaggcaacac tttgaagccg
    26761 tctttaatca ggatttccgc cgcttcgacg agttggaaca cgtccggctg caaggtgtcg
    26821 tcgtcgccga tgagttccaa ttttatccaa tcggtttcaa acacttcgcg cgccatttgc
    26881 gccgtcgtta ccgcttcctg cacgctttgg cagcctgccg tgttcggcag gacgggaacg
    26941 ccggtttctt gaagcagcga ccaaaacccc tgaccgtgcg cctcgccgcc gcatcccgtg
    27001 cggcgcagcg agacggtaat catcgcgggc cgggcggttc ggacggattg tttgaggatt
    27061 tcaggggtcg ggtaggcggc cgtgccgagc agcagccgcg aagggaaagt ttcgccgtac
    27121 agggtgagca tgatggattc tttggtatgg ttgtcggacg cgatgccgtc tgaaagccgg
    27181 cggcgtgcct ttggggtttc agacggcatt cagccgccga cgacggggcg gacgatgtcg
    27241 attttgtcgt tttcgtgtaa aacggtttcc gcatacgcgc ctttggggat gaaaacggtg
    27301 ttgaccgcca cggcaaaggg cttttgcggc gcggtttggg cgatgaggtc ggcaacgctt
    27361 gtgccgtgaa gttcggcggg tccgccgttt aagatgatgt tcatggtttc tcgatgtttc
    27421 tgtatttata gtggattaac aaaaaccggt acggcgttgc cccgccccgg ctcaaaggga
    27481 acgattccct aaggtgccca agcaccgggc gaaccggttc cgtactattt gtactgtctg
    27541 cggcttcgtc gccttgtcct gatttttgtt aatccactat atctagccga attactttat
    27601 tttttgatac gcaaccggcc ggttgccgtc attcccgcgc aggcgggaat ccatttttga
    27661 aattcggaaa ctgtttttca aatgatggtt tctcaagttt tacggcggat tcccgcctga
    27721 gcgggaatga cggaatttga tgatatgcca catttaaagt taagcatatt tgcgataacg
    27781 ggctaagttt tgcctacacg gcaattgaat tttcttcttt cggttttatc catcccacaa
    27841 agcctgaaac gctttaacca ccgcttcggg atttgccgct tcggttacgg cgcggacggc
    27901 ggcgagtgag gaaacgccgg tggccagcac ggctcgggcg ttgttcaaat cgatgccgcc
    27961 gatggcgacg acgggcgtgc cgcgagcctg ttccacatat tcgcgcaatt tgtccaagcc
    28021 ttgcggggcg gtgggcattt gtttggtcgt ggtctggaaa atcgcgccgc tggcgatgta
    28081 gccggggtgt acggacaggg cgcggtcgag ttcggcgacg gagtgcgtac tcaaacccaa
    28141 acgcaaaccg gcggcggcga tggcggcaag gtcggcggtg tccatgtctt cctgcccgag
    28201 atgtacgccg tatgcgcccg cttcgattgc ctcgcgccag tggtcgttga tgaaaagctg
    28261 tgtgcggctg ccttgacagg cggcgacgca gcgggcgatt tcgcgtttca attcgttgcc
    28321 gtgcaaggtt ttacagcgca gttgcaccgt gtcggcacct gctttgacca tgcgccccac
    28381 ccaatcggcg gtgggaacga cggcgtagaa tttaagtagg gattttaggg gcgggaaggt
    28441 catagggtgt cctttcgggc ggctttaatc ttgtcttccg atatacgcca aaccgctttc
    28501 ttcatcacgt tcgggcgcgt cttttccgtc aaacagtgcc actgccaatc tgacggcggc
    28561 ggcggttacg gcgggggaaa tcataaagcc gtgccggaaa aggccgttga tttcgatgag
    28621 gcggcgttcg cggctgtagc ggatttcggg gttgtggtgg ttgagcgtgg ggcgcaggcc
    28681 ggcggcgatt tcgaggatgt cggcttcgcc gaaggcgggg tggacggcat agagcgcgga
    28741 taagagttcc agcccggaac gtacgctggc gggggcttgg ctttcgcttt cgatttgggt
    28801 cgcgccgatg acgaagacgt ggttttcttt cggggcgatg tagagcggat agcgcgggtg
    28861 cagcaggcgc acggggcggt tgagcgtgat ttcgggcgtg taaacccgcg ccacttcgcc
    28921 gcgtatgccg cgcaaggtgc tggtgtgctc gggggattgg ttccacgcgg ttttcgcgcc
    28981 gtagccccgg cagtcgatta cccagtcgta ttgggcttgc aggtcttggg gggcgcattc
    29041 gtgttcccaa tggcaaggga cgttcagttc gtccaaagcg tcggcaagtg cagacaatat
    29101 ttgccgcccg tcgagctggc cttcggtcgg caggtagatg ccgtctgaaa aacgtccgcc
    29161 gagttgcggt tcgcgttcgg cgatttcatc ggcgcgccaa cggacgattt cgtcatccgc
    29221 tacgccgccg cgtttgagat ggcggacgaa ctcgctggat aatggcttgt cctgcccgtg
    29281 ccacacaatc aggctgccgt tttcctgcat catcgtgagc gtgttcagac ggcatcggat
    29341 gccgcgccaa agcggaatgc tctgcctgcc cagcctgatg acttcgggcg ttgcctcgac
    29401 cgcttccgcc gcaggcgcga gcatcgcggc ggcaacatag gcggcggcgt gttcgccttg
    29461 gcgggtgccc ttgtcgaaaa gttcaatctg ataaccttgt tctgcaagct gcaatgcggt
    29521 cagccttccg gaaaggccgc ctccgaggac ggcgatacgg gtcatgacgg gttcctttgt
    29581 aaagattggg tttttttaaa gaaaaggcgt accgatacgg tggcaatggc aacggcagac
    29641 attacggggg cggtcagacc gatgctgctt tcccaaccgg acgagagcag gaagcggtag
    29701 aggatgaagc ctgccagcca cagaaccagt ccggcaaagt caaagccttc aatctcctca
    29761 cgccgtttta agacgaaaaa gtcggcaatc aaaaccgccg ccatcggcgc aaataccgag
    29821 ccgataagca gcaggaagtt tttatattcg gtaacgggca gcatgacggc aagcaccgtg
    29881 ccgatcaggg taacgccgac agcgacgggt atttccgcaa aacgcgcgga aatgttgttc
    29941 gcactcgcgc cggcggaata ggtatcgaga aacgttgtgg taacggtgga gaggacgact
    30001 gccagaatgc ccgttatgcc caagcccgcg cccaacagga ttttcgccac gtcggtttct
    30061 ccggtaaaca gagccgccgc caaacccaag gcatacatcc agcagcccgt cagcgtatag
    30121 gcgagcgttg ccgtcagggt tgccgcaaac gggcggcgtg cttggcgcgt gtagtcggcg
    30181 gccagcggca gccaggaaag cggcatgacg gcggacagtt tgactgccgt tccgaaggtc
    30241 atgccgtctg aaacggcggg cgcggcgttt gtgccggacg aagcgaacac ttcgacgctc
    30301 aaccacaaca cggcaagcag catcagcagc atcgaaacgg ttttcagccc gcccgttctg
    30361 cgtgcgccga aaaccagcca cagcacgatc agtgcgccgt ttgccaatgc ccaccagaca
    30421 aaggattcgc cgtcccacaa cactttgccc aaagcggagc tgaccgttgc gccgacgtaa
    30481 atcatcaccg ccgtccagcc ggccagttgc agcatattcg ccacggaaaa cagcactgaa
    30541 ccgcatttgc cgaacgacag gcgcacactt tccatcgagc tgcgtccggt cagtgcgccg
    30601 atatacgctg ccgcaaaaaa cagcgcgccg ccgacggcat gacccaaaag cagggccgcc
    30661 agaccgcgct gccagcccaa gggggcgagc agcgtacccg tgctgatttc ggcaatcgat
    30721 accgccgcgc cgaaccaaat cagcccgatg gcggcggaag atgaaggaga ggaggcattg
    30781 cccgacatat gacgtccttt aaaaagaagg aaggtgggcg gggaagtgcg gaaaagggaa
    30841 cggaagcccg aaagggcggg gaggttttgt ttcctacgca ggcattaccc cgacaggttc
    30901 tacggatttt gctttcgcaa tctcagccgc ccggcgggcg gcactccgac aagaaggctt
    30961 taagtttaca caaagcaacg tgcgttggca aaattcttac attttcgacg gcacggcgca
    31021 tttccataac aaatgccgta taatcaagcc cctgattttt tatgcgccga cactatgcat
    31081 ccgacctatt ccgccgtaca ggcgcggctg ctcgaagcca accgcctttg ccccgaactg
    31141 ctcgccaaaa gcctgtgcat catcggcgcg caccacgtcg attatgccga tatctactgc
    31201 cagcgcaccg cttatgaaag ctggcatttg gaagagggca tcgtcaaatc gggcagcttc
    31261 caaatcgatc agggtgtggg cgtgcgcgcc gtttcgggcg acaaaaccgc ctttgcctac
    31321 gccgacagcc tgtgcatcga ttcgataaac cgttccgccc gcgccgtccg cgcgattggg
    31381 gcggcaggcg gcaaggtgtc cgccaaaatg ccgtctgaaa cgcgcggcaa gccggtttgt
    31441 tccgcatccg accccattgc cggcctcgat tccgccgcca aagtcgcgct gttgaacaaa
    31501 gtggaagcaa tcgccaaagc cgccgatccg cgcatcgtgc aagtgatggc cggtttgacc
    31561 tgcgaatacg atatggttta cctcgcccgt ctggacggca aacacgccgc cgacatccgc
    31621 ccgatggtgc gcctgaacgt taccgtcatc gccaaacagg gcgaacgccg cgaacagggc
    31681 ggcgcgggcg gcggaggacg ctacgacttg gcttatttcg atgaaacctt ggtgcggcag
    31741 tttgtcgatg ccgccgtcaa acaagccctg accaatctcg aatcccgtcc tgcgcctgcc
    31801 ggcgagatga ccgtcgtttt gggcaacggt tggccgggcg tgttgctgca cgaagcggtc
    31861 ggacacggtt tggaaggcga tttcaaccgc aaggggacca gcgtcttttc cggccggatc
    31921 ggcgaacgcg tcgccgccaa aggcgttacc gtcgtcgatc agggcgatat tgccggccgg
    31981 cgcggttcgc tcaacatcga cgacgaaggc aacgaaacac gccgcaccgt attgattgaa
    32041 gacggcatct tggtcggcta tatgcaggac gaaaccaacg cccgcctgac gggtacgcaa
    32101 tcgaccggca acggccgccg ccaaagctac gcctccgtcc cgatgccgcg tatgaccaac
    32161 acctttatgg aaaacggcag ctacgagccg gaagaaatca tcgcgtccat cgacaagggc
    32221 atttacgccg tcaacttcgg cggcggacag gtggacatca ccagcgggaa atttgtcttc
    32281 ggcgcgtccg aagcgtggtg ggtggaaggc ggcaggctgc aataccccgt caaaggcgcg
    32341 accattatcg gcaacggccc cgaagtgctg aaacacgttt cgatgatagg caacgatacc
    32401 gcgctcgaca gcggtgtcgg cgtgtgcggc aaagaagggc agagcgtccc cgtcggcgtg
    32461 gggcagccga ccctgcgtat cgatgccggg ctgaccgtcg gcggcagcgc aatctgacgc
    32521 ggaatccggc acgatgccgt ctgaaaggtt ttcagacggc atcgccttat tcttttttcc
    32581 gatgtttgat attcctaagc attctttaac attatcttac aagttcatta aaataccgcc
    32641 tttcgttaag ctgatttctg atgaactggc cttatttaat cgatgccgta cccaaattcg
    32701 ccgatgcggc aaagctgacg ctggaattgt ccgtttacgg cgttgttttg tcgttgctgt
    32761 tcggcctgcc cgtcgccgtg gtaacggcat accgcatccg ccccttctac gcattggcgc
    32821 gcgcctatat cgagctgtcg cgcaatacgc ccctgctgat ccaattgttt ttcctatatt
    32881 acggtctgcc gaaaatgggc atcaaatggg acggtttcac ctgcggcgta atcgcgctgg
    32941 ttttcttggg cgcaagctat atggcggaag ctgtccgcgc cggcatcctc gccgtcccca
    33001 aagggcaggt cggagcgggc aaggcaatcg gtttgagccg ctttcaagtg ttccgctatg
    33061 tcgaattgcc gcaggtttgg gcggtcgccg ttcccgccat cggcgcaaac atattgtttt
    33121 taatgaaaga aacatccgtc gtcagcacgg tcggcattgc ggaattgtta tttgttacca
    33181 aagacgtcat cggtatggac tacaaaacca atgaagcctt gttcctgctg tttgccgctt
    33241 atttgattat cctgctgcct gtttctttgt tggcgcggcg gattgaaaac cgcgtacgga
    33301 gtgcgaaata tggcgtttga atggctgttt gaaggaaaaa atgcggcacg gttgggcgaa
    33361 ggcctgctct taacggcaca aatttcttta atctctgttg cggcttcttg cgtattgggc
    33421 acgctgttcg gcttggtttt gcgttcgcgc aaccggcttg tccgctttgt cggacggttt
    33481 tatctcgaaa ccatccgaat cgtgccgatt ttggtgtggc tgttcggcct gtatttcggc
    33541 ctatccgtct ggacaggcat ccatatcggc ggattttggg tctgcgtctg ggtattttcc
    33601 ctgtggggcg ttgccgaaat gggcgacttg gtgcgcggcg cactggaatc gattgaaaaa
    33661 caccaggtcg aatcgggtct tgcgcccggc ttgagccggg ggcaggtatt ccgctgcatc
    33721 gagctgccgc aaagcatccg ccgcgtattg cccggcgcgg tcaacctgtt cacgcgcatg
    33781 atcaaaacca gctcgctcgc ctggcttatc ggcgtgatcg aagtcgtcaa agtcgggcag
    33841 caaattattg aaaactcgtt gctgacgcag cccaatgctt cattttgggt ttacggcctg
    33901 atttttatgc tgtatttctt ctgttgctgg ccgctatctc tactggcggc aaaacttgaa
    33961 caaaaatggg aacactgaca tggctttact gagcatccgc aagctgcaca aacaatacgg
    34021 cagcgtaacc gccatccaat ccttagactt ggacttggaa aaaggcgaag tcatcgtact
    34081 gctgggcccg tccggctgcg gcaaatccac cctcctgcgc tgcgtcaacg gtttggagcc
    34141 gcaccaaggc ggcagcatcg tgatggacgg tgtcggcgaa ttcggcaaag acgtttcctg
    34201 gcaaaccgcc cggcaaaaag tcggtatggt ctttcaaagc tacgaactgt ttgcccacat
    34261 gaccgtcatc gaaaacatcc tcttaggccc ggtaaaggta caaaaccgcg accgtgccga
    34321 agcagaggcg caagccggca aactgttgga acgcgtcgga ctgctagacc gcaaaaacgc
    34381 ctatccgcgc gaactttccg gcggtcagaa acagcgcatc gccattgtcc gcgccctgtg
    34441 cctgaatccg gaagtcatcc tgctggacga aatcaccgcc gcacttgacc ccgaaatggt
    34501 gcgcgaagtc ttggaagtgg ttttggaact cgcccgcgaa gggatgagta tgctcatcgt
    34561 aacccacgaa atggggttcg cacgcaaagt tgccgaccgc atcgtcttta tggacaaagg
    34621 cggcatcgtc gaatcgtccg accccgaaac ctttttttcc gcaccaaaaa gcgaacgcgc
    34681 ccgccaattt ctggcaggta tggactactg atttaccgca aaccgtccga acccttttca
    34741 gacggcattt catagggcag tgctttatgt gtcgtcctgt gtgttgaaac atcccaagca
    34801 aacattcaaa aaggagattt ccaatgaaat tgaacgccaa actcaaagcc cttttggctt
    34861 ccgccgccat cgccgtcggt ctgaccgcct gcgggggcgg ctccggcgat gcccaatctt
    34921 cacaaagcag cggtgcggca accgttgccg ccatcaaaga aaaaggcgtt atccgcatcg
    34981 gcgtattcgg cgacaaaccc ccgttcggct atgttgacgc aaacggcaaa aaccaaggct
    35041 ttgacgttga aatcgccaaa gacctggcca aagacctgct cggcagcccc gacaaagtcg
    35101 aattcgtcct gaccgaggcc gcaaaccgcg tcgaatacgt ccgttccggc aaagtcgacc
    35161 tcatcctcgc caactttacc caaacccccg aacgcgccga agccgtcgat ttcgccgatc
    35221 cttacatgaa agtggcgttg ggcgtggttt cccccaaaaa caaaccgatt accgacatgg
    35281 cgcaattgaa agaccaaacc ctgctggtca acaaaggcac caccgccgac gctttcttca
    35341 ccaaaagcca tcccgaagtc aagctgttga aattcgacca aaacactgaa accttcgacg
    35401 cgctgaaaga cggtcgcggc gtagcactcg cgcacgacaa cgccctgctg tgggcgtggg
    35461 cgaaagaaaa cccgaacttt gaagtcgcca tcggcaatct cggtcctgcc gaatttatcg
    35521 cccccgccgt tcaaaaaggc aacgccgacc tcctgaactg ggtcaacggc gaaatcgccg
    35581 ccatgaaaaa agacggccgt ctgaaagccg cctatgaaaa aaccctcttg cccgtgtatg
    35641 gcgagaaagt caaaccggaa gcattgttgg ccgaataagt ttgtttgaca agaaaatccg
    35701 tatccgcatt tgaaggcggg tgcggatttt tttcagccgc cgaaaatatt gcaaattcaa
    35761 aggaaaaatc aactatctca tcgttttatt tattttatta ttcttgcgcg tgcggaaatc
    35821 aagttcgtcc ggttttgcct gtttttgaat ttcgggaaac ttccaaatcg tcattccaga
    35881 taaaacgctt cagccttatg tttccggatg accgttttcg cgggatgacg gcggagcgtt
    35941 tctgttattc ggataaattc ctacggtatt gaaacggcga taaggtgttt tcctttatta
    36001 tataatttca atgcaaaatg atattttttt gaacaagcgt gccgcaaagc cggaaaaaat
    36061 gttcaagccg catcggcggc agacgggacg aaaaatgacg tttgacgaat ggttgggctt
    36121 gtcaaaactg cctaaaattg aagcaaggat gctgctgcaa tatgtttcgg aatatacgcg
    36181 cgtgcagttg ttgacgcggg gcggggaaga aatgccggac gaaatccgac agcgggcgga
    36241 caggctggcg caacgccgtc tgaacggcga gccggttgcc tatattttag gtgtgcgcga
    36301 attttatggc agacgcttta cagtcaaccc gaacgtgctg attccgcgcc ccgaaaccga
    36361 acacttggtc gaagccgtat tggcgcgcct gcccgaaaac gggcgcgtgt gggatttggg
    36421 gacgggcagc ggcgcagttg ccgtaaccgt cgcgctcgaa cgccccgatg cgtttgtgcg
    36481 cgcatccgac atcagcacgc ccgcccttga aacggcgcgg aaaaatgcgg cggatttggg
    36541 cgcgcgggtc gaatttgcac acggttcgtg gttcgacacc gatatgccgt ctgaaaggca
    36601 atgggacatc atcgtgtcca acccgcccta tatcgaaaac ggcgataaac atttgtcgca
    36661 aggcgatttg cggtttgagc cgcaaatcgc gctgaccgac ttttcagacg gtctaagctg
    36721 catccgcacc ttggcgcaag gcgcgcccga ccgtttggcg gaaggcggtt ttttgttgtt
    36781 ggaacacggt ttcgatcagg gcgcggcggt gcgcggcgtg ttggcggaga atggtttttc
    36841 gggagtggaa atcctgccgg atttggccgg tttggacagg gttacgctgg ggaagtatat
    36901 gaagcatttg aaataattgt ttgcaaaatt ggcggatttt ccgttattgg gttatgatgt
    36961 gttttgatat gtaaacattt ataggtttgg agcgataaac aatgaatgca gtcgtagttg
    37021 ccgtaatcgt tatgctggtg ctgtcgctgt cgcgcgtgca cgtggtattg agcctgacgg
    37081 tcggcgcgtt tgtcggcggc gcggtggcgg gtatgccgct gcaaaacatt gccgatgcgg
    37141 cgggacaggt cagtcaggcg gggattatcc ccgtgttcaa caaaggtttg gaaggcggtg
    37201 cgaagattgc gctttcttat gcgatgcttg gtgcgtttgc gatggcgatt acccattccg
    37261 gcctgccgca gcagcttgcc ggcgcggtcg tccgcaagct gaaccggggc ggtatgcccg
    37321 acagcgtgcg ttcgggcgag ggcgcggtca aatggctgct gctttccatc atccttgtga
    37381 tgggcattat gagtcagaac gtcataccca tccacatcgc ctttatcccg atgattgttc
    37441 cgccgctgct tttggtgttt aaccgcctga aaatcgaccg ccgcctgatt gcgtgcgtca
    37501 tcactttcgg gctggttacg acttatatgt tcctgcctta cggcttcggc gcgatttttt
    37561 tgaacgaaat cctgttgggc aacatccatt ccgccgcgcc gcagcttgat gtgaaaaaca
    37621 tcaacgtgat ggcggcaatg gcgattcccg cattgggaat gctggccgga ctcctgctgg
    37681 cgtttgtcca ctaccgcaaa ccgcgactgt accaaagcaa caatgccgat acggcgggca
    37741 acgccgatgc ggcaaaccgt ccgcagccgt ccgcctaccg cagcctggtt gccgccgccg
    37801 ccatcgccgt atgctttgcc atacagttga tgtatgaaga ttcgctggtg ttgggcgcga
    37861 tgctcggttt cgccgtattt atgatgttgg gggtcattaa ccgcgacaag gcaaacgacg
    37921 tattcggcga aggcatcaag atgatggcga tggtcggctt cattatgatt gccgcgcagg
    37981 gttttgccgc cgtgatgaat gcgaccgggc atattcagcc gctggtggaa agcagtatgg
    38041 cgatattcgg caacagcaaa ggtatggcgg cgttggcgat gctggtggtg gggcttttgg
    38101 taacgatggg catcggttcg tccttttcca ctttgccgat tattgccgcg atttatgtgc
    38161 cgctgtgtac cggtttgggc ttttcgccgc ttgccaccgc cgccattgtc ggcacggcgg
    38221 gggcgttggg cgatgccggt tcgcccgcgt ccgattccac gctgggcccg acgatggggc
    38281 tgaacgccga cgggcggcac gaccatatcc gcgattccgt catcccgacc ttcatccact
    38341 acaacatccc gcttctgatt gccggctgga ttgccgcgat ggtgctgtaa atggacgcgg
    38401 ttcaagagtt ggaacgccgt attgtcgaac tggaaatcca aaccgcgctt caggaggacg
    38461 taatctccgg tctgaacgcg atggtggcgg aattgcggca gacgttggat ttgcagcagg
    38521 ctcagttgag gctgctgtat caaaaaatgc aggacaggaa tcccgacgcg caagagccgt
    38581 attccctgcg cgacgagatt ccgccgcatt attgatgcgc cgccgtatcc ggatttcctt
    38641 aaataaaggc tgtgtttgaa tattccgccg gtgcaaccct aagatgtaac cttaatcagt
    38701 cggacaaaat gccttttatc cgatgaaacc ggttcttgtc cgaacggcga tgagcggctt
    38761 cccccgctgc aaacagataa aaccaaccgg gtattcaaac acagccaaaa aaagccgtcc
    38821 gaacccaaag gggacagacg gccaaacaca ttacggggaa aacgtcttac tcaatgagtc
    38881 tgcgaaacag acattgctaa aacaactgca attataaggt aagtttattt ttttgcaaag
    38941 aaatgtaaat ttttaaattt ttaaaaccgt tccgccgcat ccgttcggat gccgtctgaa
    39001 acggcggcag ggttttaccg tgccgcgccg caaaccgggc attcggggtt gcgcggcagg
    39061 tcgaaatatt gccagccccc ttccaaggca cggtaaaccg ccagcctgcc gtgcgacggt
    39121 tcgcccgctt ccagcaggat tttcagagcc tccgccgcct gtgtgcagcc gacgatgccg
    39181 accagcggcg agagcacgcc gaagagggaa cagatgccgt ctgaagccga tccgccgtcg
    39241 aacaggcagg cgtaacacgg cgagtcgggc aagtcgggac ggtatgcggc aagctgtcct
    39301 tcaaaacgta ccgccgcccc tgaaaccagc ggtgttttcg tttgcacgca ggcacggttg
    39361 accgcctgcc gcgtggcgta gttgtcgcag cagtctaaaa cgatgtcggc ggtttgaacc
    39421 aaaccggtca ggcggcagcc gtcgagtttt tcgttgacgg cgcggacatc gacagtatgg
    39481 ttgatgcgtt tcaggcggcc tgccaaggtt tcggctttag gcttgccgac atcgccctcg
    39541 tcaaatgcga cttggcgttg caggttgtgc agctcgaccg tgtcggaatc ggctatggtc
    39601 agcgtgccga cgcccgaggc ggcgagatag ggcagggcgg cggcgcccaa tccgccgcag
    39661 ccgacgacca aaatatgcgc ggcggaaagc ttctgctgcc cttcgatgcc gatttcgtcc
    39721 aagaggatgt ggcggctgta ccgcagcagg agtgcgtcgt cgttgtcgtg ttcggtcgcg
    39781 gtcatgatga tgttcggaaa aaacagttgc gggcgattgt aacgccgccg tcgggcggcg
    39841 ttcaacttca gacggcattt cgggacatga gtggttaaag tgtgaacggt ttggcacgga
    39901 tgcggcattt gaggtacatt tacagtattt acggcaatga gaggaaaatc atgcaactgc
    39961 atatcctgaa caatccgaag gacgcggctt tggcggcgga cgcggaattt ttgaaacagt
    40021 ccctgttcaa cctcctgcac gaagaagcct cgccgttggt tgtcgaaacg gtcaaactct
    40081 tgtccacttc cgacgacagc gcggcattga ttgaaaaagt attgccgcaa ttggacgaac
    40141 ggcagaccta cgatctgacg cttgcctgcg ggctgttcgc ccagattctg aatatcgccg
    40201 aagacgtgca ccacgaacgc cgccgccaaa tccacgaaga tgccggccac aacgcagcag
    40261 aaggcagcct gacggaaacc gtccgcaggc tcaaagcggg gaaagccgac ggcaaatcgg
    40321 tgcagcggca gttggacaat acgtccgtta ccgccgtttt gaccgcgcac ccgaccgaag
    40381 tgcaacgcca aaccgttttg aatttcaacc gccgcatccg cgcgctgctg ccgcaacgcg
    40441 aacgctgcac caatgccgac gcgctggcac ggctgcgccg cgaaatcgac accgtgcttc
    40501 tgggcttgtg gcagaccagc gaaacgcgcc gccacaaact cagcgtcaac gacgaaatca
    40561 acaacggcgt gtccatcttc ccgatgagct tttttgaagc cctgcccaaa ctctaccgca
    40621 agatggaaca cgactttcag acggcctatc ccgatgtccg cgttccgaac atccttaaaa
    40681 tcggcggctg gatcggcggc gaccgcgacg gcaatccgtt tgtttctggc gaaaccctgc
    40741 gctttgcctt ccgccgccac gccgatgccg tgttccgttt ttaccgcagc gaactcgaca
    40801 aactttaccg cgaactgccg ctttccatcc gccgcgtcaa agtcaacgac gacgtaatgg
    40861 cactggcagc cctctcgcct gacgaagaaa tcgcccgcac cgaagaacct taccgccgcg
    40921 ccattgccta cattatggcg cgcgctatgg gcaaagcgcg ctcgctcggt ttgggcatgg
    40981 gctgcaaatt cggctttctc gagccttatg cttcggccca aaaatttctc gatgatttga
    41041 aaaaattgca acgttccctt atcgacaacg gcagccgcct gcttgccgaa ggccgtttgg
    41101 cagacctcat ccgttccgtg tccgtgttcg gcttccacat gatgccgctc gacctgcgcc
    41161 aacacgcagg caaacacgcc gatgtggttg ccgagctttt ccaacacgca ggcttggaag
    41221 actacaacag cctgaacgaa gagcaaaaac aagccgcctt gttgcgcgaa ttgagtcacc
    41281 aacgtccgct gtacagcccg ttcatcacat acagcgacca tacccgccgc gaattggcga
    41341 ttttcaacga agcgcgcaaa atcaaagacg aattcggcga agatgcctta acacaaagca
    41401 ttatttccaa ctgcgaacaa cccggcgacc tgctcgcctt ggcattgctg ctgaaagaaa
    41461 gcggcctgtt ggcggtggaa aacggcaaac cgcacagccg catcaacatc gtgcctctgt
    41521 ttgaaaccat cgaagcgctc gaaaatgcct gtccggtcat ggaaaccatg ttccgcctcg
    41581 actggtatga tgccctgctc gaaagccgcg gcaacatcca agaaatcatg ctcggctatt
    41641 ccgactccaa caaagacggc ggctacgtta ccagctcatg gtgtctgcat caggcggaat
    41701 tgggcttggt cgaactcttc aaaaaatacg atgtccgtat gcgcctgttc cacggtcgag
    41761 gcggcagcgt cggacgcggc ggcggcccgt cttaccaagc catcttggcg caacccgcag
    41821 gcagcgttgc cggacaaatc cgcattaccg agcaaggcga agtcatcacc gccaaatacg
    41881 ccgaccccgg caacgcccaa cgcaacttgg aaaccttggt tgccgcgact ttggaagcca
    41941 gcatcctgcc ggataaaaaa gaccctgatg cgaaactgat gcaggcattg tcggacgtat
    42001 cgttcaaata ctaccgcgaa ctgattaccc accccgactt catcgactac ttcctgcaaa
    42061 ccagcccgat tcaggaaatc gccaccctca acctcggcag ccgtcccgcc agccgcaaaa
    42121 ccctggcgcg aattcaggat ttacgcgcga ttccgtgggt attctcctgg atgcaaaacc
    42181 gcctcatgct gccggcttgg tacggtttcg gcagcgcggt ggaaaccttg tgcgaaggca
    42241 gccccgaaac cctcgccgcc ctgcgcggac acgcccaaaa caaccccttc ttccaagcga
    42301 tgctctccaa tatggaacaa gtgatggcga aaaccgacat caccttggcg gaaaactacg
    42361 ccggtttgag cgaatcaccc gaaaaggcaa aagtcatctt cggcatgatt aaggaagaat
    42421 accgacgcag ccgcaaagcc ctgctcgacc tgctgcaaac cgaagaactt ttgcgcgaca
    42481 accgcagcct cgcccgttcg ctcgctttga ggattccata cctgaacgcg ctcaacggtt
    42541 tgcaagtcgc catgctcaaa cgcctgcgca aagaacccga caatccgcac gccctgctga
    42601 tggtgcacct gaccatcaac ggcgtggcgc aaggtttgcg caataccggc tgatagtgcc
    42661 gcatcggggt aaaatgccgt ctgaacgcct ttcagacggc atttacctga ccgcacttgc
    42721 agagaaacac cgattgtttt aaagtgaacg gcagtgatat gttgaaagac gaccaatgaa
    42781 aattaccgtt atcggcgcag gttcgtgggg tacggcgctc gccctgcatt tttcccaaca
    42841 cggcaaccgc gtatccctgt ggacgcgcaa cgcagaccaa gtccgccaaa tgcaggaagc
    42901 gcgtgaaaac aaacgcggac tgcccggctt ttcctttccc gaaaccttgg aagtgtgtgc
    42961 ggatttggca gaggcgctca aagacagcgg acttgtcctt atcgtaacct ccgttgccgg
    43021 attgagaagc agcgcagagc tgctcaaaca gtacggcgcg ggacacctcc ccgtcctcgc
    43081 cgcctgcaaa ggcttcgagc aggatacagg gctgctgacc ttccaagtct tgaaagaagt
    43141 attgcccgac aataagaaaa tcggcgtact ttccggtccg agttttgcac aggaactcgc
    43201 caaacaactg ccctgcgccg tcgtcctcgc ctccgaaaac caagagtgga ttgaagaact
    43261 cgtaccgcag ctcaacacga ccgtcatgag gctttacggc agtaccgacg ttatcggcgt
    43321 ggcagtaggc ggttcagtca aaaacgtcat ggcaatcgct accggactct ccgacggact
    43381 cgaatacgga ctcaacgccc gtgccgcact ggttacgcgc ggactggccg aaatcacccg
    43441 ccttgcctcc gcaatgggcg cgcagcccaa aaccatgatg gggcttgccg gcatcggcga
    43501 tctcatcttg acctgtaccg gtgcactttc gcgcaaccgc cgcgtcggct tgggtttggc
    43561 agaaggcaag gaactgcatc aggtgctggt cgaaatcggg cacgtttccg aaggggtcag
    43621 caccatagaa gaagtcttca ataccgcctg caaataccaa atcgatatgc cgattaccca
    43681 aaccctgctg caactcatcc gcaaagaaat gacccctcag caggtcgtcg aaaggctgat
    43741 ggaacgcagc gcgcgtttcg aataaacaac aaacagatgc cgtctgaacc cccagacggc
    43801 atacagacag gtaaggtcat gaaacaaaat atcgaaaaac tcgaaagcag cgtttatacg
    43861 ttggtacaaa aattcgaaac cctcgtcagc gaaaaccgcc gcctcaaaga aaccgtcgcc
    43921 gaactcgaac gggcgcacga gcggcaaaaa ctcgaacacg agaccgccgt cgacgaactc
    43981 agcgaagccc tgctcgtcca agtcggcaaa ctcaaagaag acctgcaaaa caaaatcgac
    44041 agcctgacgg aagaaaatgc acgataccgc gccctgctcg aacagagcag ggagaaaatc
    44101 agcgcactgg cagcgcgcct cccccaacgg caggaaacgc agcaataagg attaaaggat
    44161 gaacatcgaa caagtctaca tcgaagtcat gcacgcccgg ctgaccgtca acacgccggc
    44221 agaagaaaaa gacacactgt tgcaggcagt cggaatgctc aacggcaaag ccgaagccat
    44281 ccgcgaaggc ggccgcgtcg cggacagcga aaaaatcgtc attatggccg cgctcaacgt
    44341 cgtccacgac ctattgaaaa cctccctgaa cggcggcgat ttggcaatcg gcgattttgc
    44401 gcgtaaaata accgatatgg acaacgcctg ccaaaaggca ctatcccgct tggggcagga
    44461 ataacccctt tttccctgcg gtgtccgcga aggcatatat tcctttgaac caataagttc
    44521 gcttaaggtt gccgggagca gtagtgggcg cgagcgtcct tttgcggacg cacccgaaac
    44581 tacccgaggc agccgccttg tcagcaaggt tcaagcggat tcggccgaaa cggctctcgc
    44641 ggggattccc attcaaaacc gcatcgcaac gatgcggttt ttcacattgg ccgccgcccc
    44701 gcccgaaaaa gcagtatgca gtctgccgaa atccggaaaa tccttccgta acgcggtaat
    44761 gcgattgaca aaatcaaatc ctgtctttaa aatccataac tttctatatc tatctggatt
    44821 tccactatga aaaccttttc agcgaaacct cacgaggtga agcgcgaatg gttcgtcatc
    44881 gatgcccaag acaaagtctt gggtcgcgtt gcaaccgaag tcgccagccg tctgcgtggc
    44941 aaacacaaac ctgaatacac cccccacgtc gataccggcg attacatcat cgtcatcaat
    45001 gcggacaaac tgcgtgtaac cggtgccaaa ttcgaagata aaaaatactt ccgccattcc
    45061 ggttttccag gcggcatcta cgagcgcact ttccgcgaaa tgcaagatca attcccgggc
    45121 cgcgctttgg agcaggctgt aaaaggtatg ctgcccaaag gtccgctggg ttacgccatg
    45181 attaaaaaac tgaaagtgta cgctggtgcg gagcatgccc atgctgcgca acaacccaaa
    45241 gttttggaac tgaaataata aggacacgac atgaacggta aatactacta cggcacaggc
    45301 cgccgcaaaa gttcagtggc tcgtgtattc ctgactaaag gtacgggtca aatcatcgta
    45361 aacggccgtc ccgttgacga attcttcgca cgcgaaacca gccgtatggt tgtccgccaa
    45421 cctctggttc tgactgaaaa cgccgaatct ctcgacatca aagtaaatgt tgtcggcggc
    45481 ggcgaaaccg gccagtccgg cgcaatccgc cacggtatta cccgtgccct gattgatttt
    45541 gacgctgctt tgaaacccgc cttgtctcaa gccggtttcg ttacccgcga tgcgcgcgaa
    45601 gtcgaacgta aaaaaccggg tctgcgcaaa gcacgccgtg caaaacaatt ctccaaacgt
    45661 tgattttgga aattcaaaaa accctgctta tcgcagggtt ttttatttgt aacaggcggt
    45721 ttcccattgg taatctaaag attacggatt gggcaaaaat caaaaacagc accagtatcg
    45781 actacacgga cgaactgacc gtcag
//
LOCUS       DAXFWM010000008        45215 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000008 DAXFWM010000000
VERSION     DAXFWM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 45215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45215
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.008"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(136..763)
                     /locus_tag="ACFI8B_000407"
                     /pseudo
     CDS             complement(136..763)
                     /locus_tag="ACFI8B_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            complement(1027..1242)
                     /gene="rpmE"
                     /locus_tag="ACFI8B_000408"
     CDS             complement(1027..1242)
                     /gene="rpmE"
                     /locus_tag="ACFI8B_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC3833707.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            1377..1787
                     /locus_tag="ACFI8B_000409"
     CDS             1377..1787
                     /locus_tag="ACFI8B_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC3833708.1"
                     /translation="MESKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLL
                     YSTNHKFDREKNALRATRKSVRLRWRTMSGRGAG"
     gene            1793..1921
                     /locus_tag="ACFI8B_000410"
     CDS             1793..1921
                     /locus_tag="ACFI8B_000410"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC3833709.1"
                     /translation="MAGVTVGRGSVVGAGAVITKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(2002..2487)
                     /locus_tag="ACFI8B_000411"
     CDS             complement(2002..2487)
                     /locus_tag="ACFI8B_000411"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC3833710.1"
                     /translation="MKKILTAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNAD
                     LQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVKD
                     YGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTALA
                     Q"
     gene            complement(2484..2867)
                     /locus_tag="ACFI8B_000412"
     CDS             complement(2484..2867)
                     /locus_tag="ACFI8B_000412"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC3833711.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            complement(2867..3229)
                     /locus_tag="ACFI8B_000413"
     CDS             complement(2867..3229)
                     /locus_tag="ACFI8B_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC3833712.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            complement(3354..4181)
                     /locus_tag="ACFI8B_000414"
     CDS             complement(3354..4181)
                     /locus_tag="ACFI8B_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC3833713.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            complement(4178..4456)
                     /locus_tag="ACFI8B_000415"
     CDS             complement(4178..4456)
                     /locus_tag="ACFI8B_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC3833714.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            complement(4507..5097)
                     /locus_tag="ACFI8B_000416"
     CDS             complement(4507..5097)
                     /locus_tag="ACFI8B_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC3833715.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            complement(5128..5622)
                     /gene="mlaD"
                     /locus_tag="ACFI8B_000417"
     CDS             complement(5128..5622)
                     /gene="mlaD"
                     /locus_tag="ACFI8B_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC3833716.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            complement(5673..6449)
                     /gene="mlaE"
                     /locus_tag="ACFI8B_000418"
     CDS             complement(5673..6449)
                     /gene="mlaE"
                     /locus_tag="ACFI8B_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC3833717.1"
                     /translation="MNFIRSVGAKTLGLIQPFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            complement(6497..7297)
                     /locus_tag="ACFI8B_000419"
     CDS             complement(6497..7297)
                     /locus_tag="ACFI8B_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3833718.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            7470..8385
                     /locus_tag="ACFI8B_000420"
                     /pseudo
     CDS             7470..8385
                     /locus_tag="ACFI8B_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8493..9924
                     /gene="aldA"
                     /locus_tag="ACFI8B_000421"
                     /pseudo
     CDS             8493..9924
                     /gene="aldA"
                     /locus_tag="ACFI8B_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(10006..10936)
                     /gene="ppk2"
                     /locus_tag="ACFI8B_000422"
                     /pseudo
     CDS             complement(10006..10936)
                     /gene="ppk2"
                     /locus_tag="ACFI8B_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
     gene            11104..12978
                     /locus_tag="ACFI8B_000423"
     CDS             11104..12978
                     /locus_tag="ACFI8B_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC3833719.1"
                     /translation="MPYFALFDDAVSGRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSSGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            complement(13039..14574)
                     /locus_tag="ACFI8B_000424"
     CDS             complement(13039..14574)
                     /locus_tag="ACFI8B_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3833720.1"
                     /translation="MMRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCV
                     WGAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRP
                     QLLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARY
                     RALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTE
                     GLTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTA
                     LADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSG
                     SDGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLARRLGGSATVFGG
                     WQFARFVPKRETVGGAVNNAAYRRNAVHIGWAQEWGGTGGLNSRIAASYAHRNYKGIA
                     AFSTEAQRNREWNVSLALSHDKLSYKGIVPTLNYRFGKTESNVPYAKRRNSEVFVSAD
                     WRF"
     gene            14817..15886
                     /gene="hupA"
                     /locus_tag="ACFI8B_000425"
                     /pseudo
     CDS             14817..15886
                     /gene="hupA"
                     /locus_tag="ACFI8B_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            15914..18343
                     /gene="hupB"
                     /locus_tag="ACFI8B_000426"
     CDS             15914..18343
                     /gene="hupB"
                     /locus_tag="ACFI8B_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC3833721.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLANDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKTYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
     gene            complement(19217..20563)
                     /gene="mnmE"
                     /locus_tag="ACFI8B_000427"
     CDS             complement(19217..20563)
                     /gene="mnmE"
                     /locus_tag="ACFI8B_000427"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC3833722.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            21001..25395
                     /gene="app"
                     /locus_tag="ACFI8B_000428"
     CDS             21001..25395
                     /gene="app"
                     /locus_tag="ACFI8B_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC3833723.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQD
                     ELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFS
                     HNRTGNTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGKIRRR
                     VLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKA
                     DYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGF
                     TLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            25533..25643
                     /locus_tag="ACFI8B_000429"
                     /pseudo
     CDS             25533..25643
                     /locus_tag="ACFI8B_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(25635..26330)
                     /locus_tag="ACFI8B_000430"
     CDS             complement(25635..26330)
                     /locus_tag="ACFI8B_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC3833724.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(26892..29684)
                     /gene="polA"
                     /locus_tag="ACFI8B_000431"
     CDS             complement(26892..29684)
                     /gene="polA"
                     /locus_tag="ACFI8B_000431"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC3833725.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            complement(29829..30335)
                     /gene="luxS"
                     /locus_tag="ACFI8B_000432"
     CDS             complement(29829..30335)
                     /gene="luxS"
                     /locus_tag="ACFI8B_000432"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC3833726.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            complement(30357..30776)
                     /locus_tag="ACFI8B_000433"
     CDS             complement(30357..30776)
                     /locus_tag="ACFI8B_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC3833727.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            complement(30809..31132)
                     /gene="cyaY"
                     /locus_tag="ACFI8B_000434"
     CDS             complement(30809..31132)
                     /gene="cyaY"
                     /locus_tag="ACFI8B_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC3833728.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            31203..31373
                     /locus_tag="ACFI8B_000435"
     CDS             31203..31373
                     /locus_tag="ACFI8B_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC3833729.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            31384..32604
                     /gene="lysA"
                     /locus_tag="ACFI8B_000436"
     CDS             31384..32604
                     /gene="lysA"
                     /locus_tag="ACFI8B_000436"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC3833730.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            32798..33220
                     /locus_tag="ACFI8B_000437"
     CDS             32798..33220
                     /locus_tag="ACFI8B_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC3833731.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRDGHIVRAQTLDASGKVEREWQP"
     gene            complement(33272..33364)
                     /locus_tag="ACFI8B_000438"
     CDS             complement(33272..33364)
                     /locus_tag="ACFI8B_000438"
                     /inference="COORDINATES: protein motif:HMM:NF028261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC3833732.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(33361..34896)
                     /locus_tag="ACFI8B_000439"
     CDS             complement(33361..34896)
                     /locus_tag="ACFI8B_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3833733.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            complement(35601..37235)
                     /gene="groL"
                     /locus_tag="ACFI8B_000440"
     CDS             complement(35601..37235)
                     /gene="groL"
                     /locus_tag="ACFI8B_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC3833734.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            complement(37328..37618)
                     /locus_tag="ACFI8B_000441"
     CDS             complement(37328..37618)
                     /locus_tag="ACFI8B_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC3833735.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            38124..40289
                     /gene="fetA"
                     /locus_tag="ACFI8B_000442"
                     /gene_synonym="frpB"
     CDS             38124..40289
                     /gene="fetA"
                     /locus_tag="ACFI8B_000442"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC3833736.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFKISDKEKGPNGTEVD
                     VDVAQKAKNRANEKIVYAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSSLNPSFGVIWQPREHWNFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNTGYIKNH
                     GYELGASYRTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            40441..41406
                     /locus_tag="ACFI8B_000443"
     CDS             40441..41406
                     /locus_tag="ACFI8B_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3833737.1"
                     /translation="MLRLTALTLCTALALGACSPQNSDPAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            41424..41615
                     /locus_tag="ACFI8B_000444"
     CDS             41424..41615
                     /locus_tag="ACFI8B_000444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833738.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            41634..42602
                     /locus_tag="ACFI8B_000445"
     CDS             41634..42602
                     /locus_tag="ACFI8B_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3833739.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGIADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFVAY
                     EFEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            42592..43566
                     /locus_tag="ACFI8B_000446"
     CDS             42592..43566
                     /locus_tag="ACFI8B_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC3833740.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFDFELVIMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWHERYRSDVHLLRRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            43611..44237
                     /locus_tag="ACFI8B_000447"
     CDS             43611..44237
                     /locus_tag="ACFI8B_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC3833741.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDSGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            44321..45079
                     /locus_tag="ACFI8B_000448"
     CDS             44321..45079
                     /locus_tag="ACFI8B_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3833742.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(45114..45215)
                     /locus_tag="ACFI8B_000449"
                     /pseudo
     CDS             complement(45114..45215)
                     /locus_tag="ACFI8B_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT        10811 a        12810 c        11750 g         9844 t
ORIGIN      
        1 agaaatggac ggattgccaa gtgtagcaaa tattcgcaca aaggtcgtgc agagactgct
       61 tcagacggca tcagacacaa aaagaccggc aacaaaaaag actgcacggg gcagtctttg
      121 cagatactat ctttttcata atattttttc ctggcccgac acagtccata gcatatcaaa
      181 actgttgttt tcaatcagga tatatatccc caatcctaaa taaacaacgg caacaaacca
      241 tctgctatat ttttccaaag tttctccgac agaagggact tgtgccaatt tttgggcaga
      301 aaaaaccaag agataaatca taactagaaa ggtaagtaaa gccactatca aattcgctaa
      361 atttaaggta gtaaaatatg ggacaaagac accaatattg tcagcgccac aacttgcaaa
      421 agtaatcata gcgactagaa aaatcaggtt tttattatct ttgcgcaaac cctctttggc
      481 aatagactct ccatcggaat ctcctaacag caaaactttg aggcctagga aaatcggaat
      541 caagccgagc aaacctaaaa tctctttact aggaatataa tctaagacaa atgcaaaaag
      601 caaacttagc aatatcaggc taacagagcc tagaaattgt cctaaataga tgttaatgat
      661 gtcttttctg cttttttttg gcaaaaaata acattaggat aataagtaag tctacggctg
      721 tcccagaata caggattatt gaagtaacaa cattttgaat cataaaacat cccattcaaa
      781 tatatttttt aatgtattca aacattaaac tttgtagata tcaacttcaa ccccgtcaaa
      841 atatagtgga ttaacaaaac cggtacggcg ttgcctcgcc ttgccgtact atttgtactg
      901 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatagat aagaagtcag
      961 tgtgccaaat attaaaaagc cctgccatcg aaatgatggc agggcttaat tcttgcaaag
     1021 cggcaatcag cgtttgaaca ggttgccgaa tttgttgttg aatttgtcca cgcggccggt
     1081 ggtatcgacg attttttggg tgccggtata gaacgggtgg cacagggagc aaacctcgat
     1141 gttaaagttt tctttttcca ttgcggattt ggttacgaat ttgttgccgc aagagcaggt
     1201 aacgttaact tcgcggtagt tcgggtgaat accttgtttc atttgatttc ctttcaaaaa
     1261 agcgggcata ggggatgtac ctatgcctca gacaagctcg gcattctcgc tattttctgt
     1321 tgtttcgtca agaggatatt cgataaaatg taaaaagttc ttttgaggaa ggtttgatgg
     1381 aatcaaaatt ctttttcctg ctgctgcgtt ttgccggttc ggtgctgccg ccgtcgcata
     1441 tgcgcggcat cggcatcatc ggcagacggg tgctcggttt tctggcgcgg cggatttcgc
     1501 cgcatatcgg acgcggggcg tatgtgtttc cggatacggt tttgggcgac ggctcgggac
     1561 gcggggcgta tgtgtttccg gatacggttt tgggcgacgg ctcgggcatc ggggcaaact
     1621 gtgaaatctg ccgtgggctg gtggtcggca aaaatgtgat gatggggccg gaatgcctgc
     1681 tctattcgac caaccacaag tttgaccgtg aaaaaaacgc tttgagggct acacggaaat
     1741 ccgtccgatt acgttggagg acgatgtctg gccggggcgc agggtgattg tgatggcggg
     1801 cgtaaccgtc ggacgcggct cggtcgtggg cgcaggcgcg gtaattacaa aagacattcc
     1861 gccctactct ttggcggcag gcaatcctgc ggtggtaaaa aagaatctgc cggaaggttg
     1921 aatgccgcct gaacgtgtcg gggcggatga tctgaaaaaa caggaacatc gtttctgttt
     1981 tttgcgcttc agacggcatc gctattgcgc cagcgcggta tcgatttctt ggtagagttt
     2041 gccgaaatcg ggttctccga cgtaggtttt gagaatttcg ccttttttgc cgataaggac
     2101 ggaagtcgga taaacctgtg tgccgaatgc ctgtccgacg gctttgtccg catcataaat
     2161 gacggtaaac ggcagtccgt agtctttgac gtattggcgg acgctttcta tcggatcgat
     2221 gggctgggca acggcgagga cttggaaatc tttatttttg tagtcgtttg ccgttttgat
     2281 gactttgggc atttcgctca cacaacccgg acaggaggga aaccaaaaat taatcagggt
     2341 gactttgcct tgcaggtcgg cgttggaaac ggtttttccg tgcaggtcgg gcagggagaa
     2401 ggcgggcgcg gttttactgt cggggatgag gacggtggca aggaggatgc cgatcagtgc
     2461 gacggcggcg gtgagtattt ttttcattcg gacaaggctt ccagtgcgcg ggcaagggtg
     2521 gcgggcaggc tgacggtgcg ttgtgtggcg gcgtggacgg gcatcagggt gatgtcggct
     2581 tctgcggcgg ttttgccgtt tggcagtgta atcgtctggg tcagcacaat gcggcgcatg
     2641 ccgggggttt tcaggcggca tgaaaactgc aatacgtcgc cttcgacggc gggacggctg
     2701 tatcggatgt cgatgcgggc gacaatcagt atgaggcctg ccaactcgtg catcagtccg
     2761 cgtttttcaa aaaacgccca gcgcgcttcc tcgagaaatt cgaggtagcg tgcattgttg
     2821 acatgaccgt agccgtcgag atggtaattg cggacggtca gcttcatcag ttcaggttga
     2881 tgggttggaa ggcttcgcgg gcaagcggtt cgtgttcgag gtcggtgatg acggtagaaa
     2941 gctggatgtc gaaccattcg ttgaaaatgt cggcatcgag tgcgggccac tcgcgttcgt
     3001 cttcgcacca gtcggcaagt tcggcggcga aaatgtcttc gaaacgggct tcgatttcgt
     3061 cccatacttc gtcggcggtt tcgcacgggc ggacaaggta ggaattggca tcggcttgga
     3121 tgtcttcgag ggtcagtccg tcgaggtggt tgcccggcag ggtttgcagc cagttccaaa
     3181 aaggttctaa agggatgagg acgaatacgc tgcggttgac ttcgtacatg gtttttcctt
     3241 tgctgtcgcg cggtatgcgc aaaaaaggga ttatagccca atctgtagtg tcggactgtc
     3301 cgttccgacg gaagggaatg ccgtccgaac acggattttc agacggcatg gctttagggt
     3361 tgtgttccag gttgcgtttc ggcttcccct gctgcttctg cctgtgtttc ggatacggaa
     3421 tcttcgtgaa cggcgggctc tgccgcgccg gtttcggcac tttcgaccaa ttcgtcgatg
     3481 tcgatgttat cttccgtacc ttcggcaggt gttgcaccgg tctgccgtgc gcggactttc
     3541 atatagaggt cgcgcgtgta gctgtatttg tcgatggcgg cttcgtccag actgtcggtc
     3601 aaatcgagca ggccttcgcg cgtactgacg gcggcagcgg cagtcgtgcc ccagcgtccg
     3661 gcaggggtat ggaaaacgat attcttgggc ggataaacag aggtaatgcc cgtgccgagc
     3721 gcgtcgcgga cggtggacgg gcctaagacg ggcaacacga aataattgct gtttttccag
     3781 ccccacgagg caaacgtgtc gcccaaagtg tttttattgt cgggaacgcc gcccgcgccg
     3841 gcaatatcaa tgagcccgcc caaaccgaag gtggtattga tgccgacgcg gacgaggtct
     3901 tcgcttgcgc gtttgatgtc caaacgcaag atattgctgc cgaaactgac cacgtcgcgc
     3961 aggttgttaa aaaaattgga cacgccggcg cggacgggtt tcggcgtaac tttgcggtag
     4021 ccgcgcgcgg caggggcgaa aatgtagcgg tcggcttggt cgttgaattt ggaaacggcg
     4081 cggttgtagc cttcataagg gtcggcgggg cgggtttctg caaatgcagg ggcggaagcg
     4141 aacccgatca gcaggaggat ggcgtaggcg gtttttttca tgatttcagc cagtctttga
     4201 tttcgtacag ttcggacagc gcgcgcacgg attcgggaat gccggtcagc ctgacgctgc
     4261 ctttgcagcc gcgcagcact tcgagcagca gcgacacgca ggcggaatcg gcacgtttga
     4321 cacctccgaa atcgacggca atggtttctt tcagacggca ttgttgccgg aaacgcccaa
     4381 atgcgtcccc ggtcagggtt ttgacggtga tgtcgccgcc gatgtgcagt gttccgtttt
     4441 tgagttctgt atgcatagcg tttgttcgaa aaacccatac cgccctcgga cggtatggtt
     4501 tgtcggttat ttgccgccgt ttttggcttt caactcggca atcagcccgt cgatgccttt
     4561 ggctttgatg atttcgccga attggttgcg gtacacggta accaggctcg cgccttcgat
     4621 ggcgacgttg taggtacggt atttgccgcc gctttggtag gtggtaaagt ccatattgac
     4681 gggcttctga ccggggatgc cgacttcggc acggacgacg atttccttgc cgcccttatt
     4741 gacgatggga ttgtctttga cgttgacggt cgcgtttttg aatttcagca tcgtgccgga
     4801 ataggtgcgg atcagcaggg tttgaaattc tttggccaac gcttgttttt gcgcgtcgga
     4861 cgcggtacgc caagggttgc cgaccgccaa tgcggtcata cgttggaaat cgaaataggg
     4921 aaccgcatag gcttcggctt ttgggcgtgc agaagccgcg tcgccgcttt tgaggatggt
     4981 caaaacctgt gtggcgtttt ggcggatttg tcccactgcg tcggccgggg aggcaaatgc
     5041 catgccgatg ctcaaaatac cgatgcccaa tgcgctgatg aaggaggatt ttttcatgat
     5101 gtctttcctg aaaatggatg tgtatgttta ttctgcggct ttttccgcat tgccgccctc
     5161 agcgtttttc tcggcgaagc tggtcatgaa tttaccgatc aggttttcca gaaccattgc
     5221 agaactggtt acggagatgg tgtcgccggc agcaaggttt tccgtatcgc cgccctgctg
     5281 cagcccgatg tactgttcgc ccaaaagtcc cgaagtcagg atttgcgcgg aaacgtcgct
     5341 gctgaactga tacttgccgt ccaaatcaag gcgcaccctc gcctgatagg atttcgggtc
     5401 aagcccgata gcgccgacgc gcccgaccaa tacgcctgcg gatttgacgg gggcattgac
     5461 cttcaaaccg ccgatgtcgc cgaaatcggc ataaacggcg taagttttgt ccgaaccgcc
     5521 gaacgccgcg ccgcccgcca cgcggaaagc gagaaaggca accgccgccg cgccgatcag
     5581 gacgaacagt ccgacccaaa attccaatat gttctttttc attaaagttc cttgaatatc
     5641 cgatgttccg cgtttcgtct tcagacagtc tgtcaatctg taaacatcca cgcggtcaat
     5701 ataaaatcga ccgccaaaat cgtcagggcg gacgaaacca ccgtgcgcgt gctggcgcgc
     5761 aaaatgcctt ccgaagtcgg gatgcagtgg aagccctgat gcacggcaat cagcgttacc
     5821 gccacgccga acgcggcgga tttgatcaaa ccgttgatta catcgtaatg tatcgtaatg
     5881 ttgttctgca tctgcggcca gaaaataccg ctgtccaaac ccagccagct cacgccgacc
     5941 aaatacgcgc cgaaaatgcc cgcgacgttg aaaatcgaag ccaaaagcgg catagaaaac
     6001 acgcccgccc aaaaacgcgg ggcaaccacg cgggcgacgg ggttgaccgc catcacgttc
     6061 atcgcttcga gctgtccggt cgttttcatc aaaccgattt cgctggtcat cgcaccgccc
     6121 gcgctgctgg caaacagaat cgccgccaac acgggaccca gttcgcgcaa cagagaagcc
     6181 gcgaccatat agcccaaaat atcggcggat ttgaatttcg acaactgcgt atagccctgc
     6241 aaacccaaaa ccataccgac gaacagcccc gaaacggcaa caatcagcac cgacagcacg
     6301 ccggcaaaat acacttggcg cacgctcagg cgcggacggg cgaaagccgt gccggatttc
     6361 gccaaaatgt tcagcagaaa cagcgtgata ctgccgaagg gttgaataag gccgagggtt
     6421 ttcgccccga cggaacggat aaaattcata aatttctata tgtaaggttc aacggtttca
     6481 gacggcacct gctcatttac ccgagcaaat cctgctgcaa cgacgtttgc gccggatagc
     6541 ggtatgccac ggggccgtct gccagcccgc cgacaaactg gcgcacccaa ggcgaatcca
     6601 gttcgcgcat ttcctgcggc gagccggaga acataatttc gccgtgcgcc aagaaaatta
     6661 cctgatcgac gatttccaaa gatttttcaa tgtcgtgcgt taccataatg ctggtcgaac
     6721 gcaaggcctt gttgacgcgg ctgatcaagt gggcaatcac gcccaaggaa atcgggtcga
     6781 ggccggtaaa cggttcgtcg tacaacataa tttcggggtc gagcgcgatt gtgcgtgcca
     6841 gcgcgacgcg gcgcgacatc ccgccggaca actcggacgg catcaggttt tccacgccgc
     6901 gcagaccgac cgcgttcaat ttcaacaaaa ccaaatcgcg aataaccgct tccggaagtt
     6961 gcgtcagttc gcgcatcgga aaagcgatat tgtcgaatac cgacaaatcg gtaaacagcg
     7021 caccgtgttg gaacaatacg cccatacggc ggcggtgttc gtagagttcg tcagccgaaa
     7081 aacccgccaa atcccgtcct tcaatcaaaa cctgcccgga ctgcggacga atctgtcctg
     7141 taatcagcct catcagcgtg gttttgccgc tgcccgaacc gcccatcacg gcggcaaaat
     7201 tgccttgcgg aatgctgaaa ttgatgtcgt tcagaatcgg gcggtcgcca tacgcgaagg
     7261 cgacgtcttt catttcgata aagggggatg ggctcatgta cgggcggacg gtaggtttga
     7321 cggcgtgtat tttaaggctt atcgggaaga cgggcaattt tcagacggca tacggacggc
     7381 aagtgttgtg aaaatgcctt tgtcggcggc ggattgtttg ctgtggcgaa aaatgttatc
     7441 tttcaaatga taacctttat cagaaaacta tggaaaaagc agaacatttg aacagcagcc
     7501 ggttcgtcaa tctggtcaaa agcggcggcg gcttatgtgg agggcagcta ccgtttcgat
     7561 actttgtcca acggcatttc catccacggc ggtacggtaa cggcacggtg cgatttttgc
     7621 agcagccgcc tcgccgaacc ttatgtgtcg ttcgtgctgt tgctggaagg gcgtttggac
     7681 ttcggcatca accgccgccg cttccgcatc gatgcggacg gcggcaagat tgtcctaatt
     7741 gccgtcgggg aagaagtcct gttcagccgc tatctttatc gcggcggcaa aacggtcaaa
     7801 atgaccatta aaggtatgga acaatggctg ccgcgtccgg aatacgcgcg tttcgcaccc
     7861 ctgctttacc gcgaaccggt caggatatgg gatttgcccc caaacctgcg cggcttggcg
     7921 gcatcctgcc tgcaaaccgt cccaaagggg catttgggcg aaacattgcg ccgcgaggcg
     7981 gacgtgttgc ggctgctgtc ggatttgtgg gacacggttt cagacggcat cgagccggcg
     8041 gcggggcaaa cggcggaagc agacgctatg ccgtctgaag acttcagccg caccctgaat
     8101 gccgcgttcg acggcggcgc acaccaagtc aaccggctga cagccgcgct gaacatcagc
     8161 gaaaggacgc tgcaacgccg tatacgcgac catttcggca ttacggcaag cgagtggctg
     8221 caccacaaac aaatgcagca cgcgctctat ctgttgcaaa acgggggcaa aaacgtgggc
     8281 gaaaccgcgt atttgtgcgg ctagcgccac gtttccagct ttattcaggc attcaggcaa
     8341 tatttcggca gcacgcctgc ggaaactaaa aaagaaaacc ggtaagccgt atttgacttg
     8401 aaacccaaaa tccgcgtgta aagaaacttg tacggtaatt tatttgggaa taaaccaaaa
     8461 ctacatccga ctacaaaact ggagggcccg aaatgaaaca attggccatg tacatcaacg
     8521 gacgctttga aaacgatttc aacggcgaat ggcgcgacgt attgaacccg tccaccgaag
     8581 aaaccatcgc ccgcgaaccc aaaggcggca gggcggacgt tgaccgcggc gcgcgcggcg
     8641 caaccggctt gggagcgtct gcctgcggtc gaacgcggcg cgtatttgcg taaaatcgcc
     8701 caaggcatac gcggacgcgc cgacgagctg accgacacca tcgttgccga aggcggcaaa
     8761 accaaagact tggcgcgtgt ggaagtgatg ttcactgccg actatctcga ttaccaagcc
     8821 gaatgggcgc gccgttacga aggcgaaatc atccaaagcg accgcccgcg cgaaaacatt
     8881 ttatcgttca aacgtccgct gggcgtgatt gccggcattt tgccgcggaa cttccccttc
     8941 ttcctgatcg cccgcaaaat gggcccggat ctggttacgg gcaacaccat cgtcgtcaaa
     9001 cccagcagcg taaccccgat caactgccac atcttcgccg aaatcatcga tgcggtcgga
     9061 ctgcccgcag gcgtgttcaa cgtggtggac ggtccgggtg cggaaatcgg caacgccttg
     9121 tccgcccatc cgcaagtcga tatggtcagc ctgaccggct cggttgaagc gggtcgccaa
     9181 gtgatggaag ccgcttccgc caacatcacc aaagtctcgc tggaactcgg cggcaaagcc
     9241 cccgccatcg tgttgaaaga cgcggatttg gatttagcgg tgaaatccat cttggcttcg
     9301 cgcgtcggca acaccggtca aatctgcaac tgcgccgagc gcgtctatgt ccacagcagc
     9361 ctgaaagacg cgttcattga aaaaatgacc gccgcgatga aaggcgtgcg ctacggcaac
     9421 cctgccgaag ccgaagcagg cgcgccggaa atgggcacgc tgattgaaga gcgcgccgtc
     9481 aaagccgttg ccgaaaaagt ggaacgggcc gtcaaacaag gtgcgaaatt ggtttgcggc
     9541 ggcaaacgca ccgaaggacg cggctatttc ttcgagccta ccctgctgac cgacaccgac
     9601 aacagtatgg acattatgaa agaagaaact ttcggcctcg tgctgcccgt ttccgctttc
     9661 gacacgctcg accaggtcat cgccctggca aacgactgcg aattcggtct gaccagctcc
     9721 gtgtacacca ccaacctgaa cgaagccttc tacgttaccc gccgcctgca attcggcgaa
     9781 acctacatca accgcgaaaa cttcgaagcc atgcagggct tccacgcggg ctgggaaaaa
     9841 tccggcatcg gcggcgcgga cggcaaacac ggtttggaag aatacctgca aacccaagtc
     9901 atttatctgg aaaccgacat ctgatgccgg tttgacacac ggatagaaaa tgccgtctga
     9961 acctgaaatc gggttcagac ggcattttta ttacttcacc ggcaatcagt catgaccgac
    10021 atcgatggtt ttctctttgt aacgcgtatc gggaacaaga acgattttat cgtccacttt
    10081 gccgatggct ttcacgtctt tacccgggta gtccaactgg tgcaggaagt agcggataca
    10141 gttcaaacgg gcgcgttttt tgtcgtccga acggatgatg acccaaggcg cgtcgccggt
    10201 gtgggtgtgg aagaacatcg cgtttttggc ttcggtgtag tcgtcccagc ggtcgagcga
    10261 ctggatgtcc acgggggaaa gtttccagtg tttcagggcg tcgtcgcggc gggagatgaa
    10321 gcggcgcagt tgttcttcgc gggatacgga gaaccagaat ttgaacaggt ggatgccgct
    10381 ggcgacaagc atacgttcca actcaggggc ttggcgcata aagagcatat acttcgttgg
    10441 gttcgcagaa gcccgtcacg cgttccacgc cggcgcggtt gtaccacgag cggtcgaaga
    10501 ataccatttc gcccgcagtc ggcaggtttt ggatgtagcg ttggaaatac cattgtccgc
    10561 gttcggtgtc agtcggtttc tccaaagcga ccacgcgcgc gccgcgcggg tttaaatgtt
    10621 ccataaagcg tttgatggcg ccgcccttgc ctgccgcgtc gcggccttca aacaggctga
    10681 cgatgcgctg gccggaatct ttgacccagc tttgcacttt cagcaattcg atttgcagtt
    10741 tggctttttc ggcttcgtag gcggcgcgga gcatacgttt gcggcagggg tagttttcgg
    10801 gcagcggggc cgtgccggaa tcttcggcag aacctttgcc ttcatgttcc aaaacagctt
    10861 tttcaaatac ctggagctgg tcttgctttt cgcctaattc tacgttttca aacggttgca
    10921 actggcggtc tgccataaag gtctcctatt gtatttcagg ttggaaatga aagtccgttt
    10981 tcacaatttt acactttcgc caccgctttc tctacataaa attacatttt gcccgaattt
    11041 gccccgccgt ctggaaatcc gtgtaaaagg ggcgtataat caaaacattt gccccgaatt
    11101 gccatgcctt atttcgccct gtttgacgat gccgtaagcg gccgcgcaaa actctatcaa
    11161 aatcatgtgg aaagccgttt tttccgtccc gaagaactcg atgctttaga cggcgcgctg
    11221 caatcgggat ggcaaaaagg gctgcattcg gtgttgtttg cagactacga attcggtttg
    11281 ccgctgatgg ggatggaatc cgaacgcggc ggcaacattg ccctgcactg gtttgccgac
    11341 tgcgccgaca ccgatgccga aagctggctt gcccgacatt cagacggcat ccccgccggt
    11401 atttccacgc cgcaatcctc cgtatccgaa accgattacc tcgaccgcat ccgccaaatc
    11461 cacgaagcca tccggcgcgg cgacacctat caaatcaact acaccacccg cctgcacctg
    11521 caagcctacg gcaaccccgt cagcctctac cgccgcctgc gccagcccgt cccctatgcc
    11581 gtattgtccc acctgcccga tgaagccggg caatccgcgt ggacgctgtg tttctcgccc
    11641 gaactcttcc tcaacatcgc ttcagacggc accatcagca ccgaaccgat gaaaggcacc
    11701 gcgccgattt tgggcgacgg acaagacgaa cgccgcgccg ccgagttaca aaccgacccg
    11761 aaaaaccgcg ccgaaaacgt gatgattgtc gatttgctgc gcaacgacct cggcaaaatc
    11821 gcccaaaccg gtaaagtatg cgtacccgag ccgtttaaag tatcgcgttt cggcagcgtc
    11881 tggcagatga ccagcaccat ccgggcgcaa gccctgccgg acacctcgtt cgccgacacc
    11941 ctccgcgccg ccttcccctg cggcagcatc accggcgcgc ccaaaaaaat gagtatgcag
    12001 attatcgaat cgctcgaaac cgaagcgcgc gggctttata cgggcagcat cggctatttg
    12061 aacccgtgtt ccagcggctt ggggtttgaa ggcgcgttca acgtcgtcat ccgcaccctg
    12121 tcgctcaaac ccgtttcaga cggcattgtt tcaggcatcg gcggcacgga ttcaaacgcg
    12181 caagcccgaa cggcaggaca aggcggggaa acgccgcatc cgtttgaagc caatccgccg
    12241 tatcggggcg tgtacggtgt cggttccggc atcgtcatcg acagcgaccc cgccgccgaa
    12301 tatcgcgaat gcggctggaa agcccgattc ctcaacgaac tgcgccccga tttcggcatt
    12361 tttgaaaccc tgcgcgcgga aaacaggcaa tgcgccctgc tcgaccgcca cctatgccgt
    12421 ctgaaaacct ccgcccaagc cctcaacctg cccctgcccg acggctgcga aaatcaaatc
    12481 aaacaataca ttgccgactt gcccgacggc gcgttccgca tcaaagccct gctcgcttca
    12541 gacggcatca gcctgtccca cgccgtttta aaccgtctga ccgacaaaca gcgcgtcatc
    12601 atttcgccga ccatcctgcc cgcacaaaac tacctgcgcc gcttcaaaac cacacaccgc
    12661 gccatcttcg accaagcgtg gcaaaccgcc gaaacgcaag gcgcgttcga cagcctgttt
    12721 ttcaattcgg acggcatcct actcgaaggc ggcaggagca acgtgttcgt caaacatcgc
    12781 ggacaatggc tcacgccctc tttagattta gacattttaa acggcataat gcgccaagcc
    12841 gtgtcggacg aaccgcaaaa atatttgcac acaaatcaag tgattgaaac acacatcaca
    12901 caaaaaacac tgcaaggagc cgaagaaatc cgcctctcca acgccttgcg cggcgtattt
    12961 gccgccgtcc ttgcctgaac gcgcaaaaat gccgtccgaa cctgctgccg caaagttcgg
    13021 acggcattat cccaccattc aaaaccgcca atccgccgac acaaacacct cgctgttgcg
    13081 gcgttttgca tacggcacat tgctttccgt cttaccgaag cggtagttca acgtcggcac
    13141 gatgcctttg tacgacaact tgtcgtggct caaagccagc gagacattcc attcgcggtt
    13201 acgctgcgcc tccgtcgaaa atgccgcaat gcctttgtag ttgcggtggg cgtaagacgc
    13261 ggcaatccgg ctgttcaaac cgcccgtccc gccccactcc tgcgcccaac cgatatggac
    13321 ggcgttgcgg cggtaggcgg cattgttgac cgcgccgccc accgtttcgc gtttcggcac
    13381 aaaccgcgca aactgccagc cgccgaacac ggttgccgag ccgcccaacc gccttgccag
    13441 cgacaccgaa agctgcgacc ggcggctcag gcggtaagaa tgcgccaact gcacacccgc
    13501 cccgtgcgcc agcatatacg gcgcaagccg gcggtcggca ggcggtttcg ttttcgcatc
    13561 aaagccgtcg ctacccgaca attgcgcctg ataaaacggc aaaaccccca ccgtctgccg
    13621 cgcattttta tactgccagc ccaaataagc cctgccgaaa gcatcgtcat aagccgattt
    13681 gcggctgaaa taatagcttg tgcccccgac attggcgcgg aacagcagat aatggttgtc
    13741 cgccagcgca gtcagctttt ccgccccggc ctcataatcc aaccctgccg cacggacagg
    13801 tccggtaacg ctgcatatcc ggctgccgcc gccctgcagg cagtattgcg gcgcggcatt
    13861 gttggcgttt ttgttcacag aagggctgat gccgcccgaa aaatgccagc ccgtcagccc
    13921 ctccgttttt ttccggaaac gccccacatt ttccaaaacc ggcgccggca aatccaattt
    13981 tgccgcctcc gcaaaatgca gccctgccga cttcagccgg aaatcgtcaa actccgccgc
    14041 cgccaaatcc agcaaaaccc gctcgtctgc cgcattatcc ctgtgcaacg cccgatagcg
    14101 cgccaccgcc tccgccggcc ttcccgccaa tttcgccagc aaagcccgcg ccctgccgta
    14161 caacaccgca tcataatccg gcagcttggc atacaaatcc gccaacgaag cgatcaaatc
    14221 cgcctgattg ccgttgagcg cgtcgcgcaa aacgtgttcc aacaactgcg ggcgcgacag
    14281 caggaaatcc ccgtcgatta cgcgcgaacc gccgtttgcg gcagaatccc cttccggcgg
    14341 cattcccgcc caaccctccg cagccgcacg ctgggcgcgc aacgccgccg catcgtcccg
    14401 aacgtcgggc aagccggcgg catacgcgcc gaccgcaccc caaacacaca gcgcggcaca
    14461 cagccggcat acccgataca cactatcctt caatttgcct tccttaaaaa acaccgccgg
    14521 acggcggcgg cagaaacgcc gcaagcccca cgcgcggcgg acgcggcgca tcatatagtg
    14581 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg
    14641 cgaggcaacg ccgtaccggt ttttgttaat ccgctataaa aaacaaaggt agaaacgcgg
    14701 cagacaaacg caggcacgtc cgatttccgc gccgccccat aaaacagttg acatcatatg
    14761 acatttaaat tgataatcaa aactattatt aatacatcat caagattgag tatgcgatga
    14821 aatacaaagc cctgccctta ctgccgcttg ccgcctgtgc cggggggggg tagccgaacc
    14881 gcacgtcccc gtgtccatcc ccaccgccac gccgctgccc gccggcgagg taacgttatc
    14941 aagcgataac ggcaatatcg aaaacatcaa caccgccggc gccggaagcg catccgacgc
    15001 gccgagccgc agcagacgct cgctcgatgc cgccccgcaa aacacatccg gcatttccat
    15061 tcggcagcgg gaagtagaaa aagactattt cggctataaa tcaaaagaaa catcattcat
    15121 cttcaaaaca cccggcggcg cgcaatacgc cctttcatcc tacgccgacc ccataaccgt
    15181 cagctactcc tcccccgatt tcaaaattcc cgaccgccac gcagggcagc ggctcgccga
    15241 cggcagccgc atctttatct gctgcagcga ctccggggct acttcgtatg cggaaattac
    15301 caaacaagat tatatgaaat tcggggcgtg gataggtccc aacggcgaaa tcgacctctt
    15361 cgccggcggc ttccccgtcg gcaaaacgcc gcctcctgcg ttctcctacg gcagcagcac
    15421 gcccgaaacc gcattgtcaa agggtaaaat cacttatcag gtttggggca tccgcgtcag
    15481 aaacgggcaa tttgttactt cctcttatac gccgccgaaa agcggttctt attatgggac
    15541 acttgccaat acccccgtcc tttcctttat caccgccaat ttcaacagca acacactggc
    15601 aggcaaaatc ctcggcaaca gcgattacgg gccggatgtg gatattcaaa acgcaacgat
    15661 taccggtccg accttttccg gcgatgccac gtccggcggg aaaagcggca aattggaagg
    15721 caagtttttt ggcaagttcg ccagcacccg cagcagcgaa gtcagcatag gcggcaaaat
    15781 cacctttgac ggcgaccgct ccctcgatac cgtattcggc ggtgtgagtt atgaaaaaaa
    15841 acttgatgac accagtcaag ataccaacca ccttactaaa caataaacca cctcatccct
    15901 gcaaaggttt cttatgccca ttcccttcaa acccgtattg gctgccgccg ccatcgccca
    15961 agcgtttccc gcctttgcgg cagaccccgc gccgcagtcc gcccaaacgc tgaacgaaat
    16021 caccgttacc ggcacgcaca aaacccaaaa actcggcgaa gaaaaaatcc gccgcaaaac
    16081 cttagacaag ctcttggcca acgacgaaca cgacctggtg cgctacgacc ccggcatttc
    16141 cgtcgtcgaa ggcggcaggg cgggttctaa cggctttacc atacgcggcg tggacaaaga
    16201 ccgcgtcgcc atcaacgttg acgggctggc gcaggcggaa agccgctctt ccgaagcctt
    16261 ccaagaattg ttcggcgcgt acggcaactt caacgccaac cgcaacactt ccgagccgga
    16321 aaacttttcc gaagtaacca tcaccaaagg cgcggactcg ctcaaatccg gcagcggcgc
    16381 attgggcggc gcagtcaatt accaaaccaa atccgcaagc gactatgttt ccgaagacaa
    16441 gccctaccat ttggggataa agggcggcag cgtcggcaaa aacagccaaa aattcagcag
    16501 cattaccgcc gccggcaggc tctttggttt ggatgcctta ttggtttata cccgccgctt
    16561 cggcaaagaa accaaaaacc gctcgaccga gggcgatgtc gaaattaaaa acgacggata
    16621 tgtctttgac ccggccaacc cctcgcccag cagatacctt acctacaaag ccacaggggt
    16681 tgctcgctcc caacccgacc cgcaagaatg ggtaaacaaa agcaccctgt tcaagctggg
    16741 ctacaatttc aacgaccgca accgcatcgg ctggattttt gaagactcgc gcaccgaccg
    16801 ttttaccaac gagctgtcta atttgtggac gggtacgacc acgtctgccg caacgggcga
    16861 ctaccgccac cgccaagacg tgagctaccg ccgccgcacc ggcgtcgaat acaaaaacga
    16921 attggaacac ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat
    16981 gaacacttgg acttgggaca tcccgaaaaa ttacgataca aacggcatca acggcgaggt
    17041 ttaccattcg ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa
    17101 acaactcgac ttttccaaag ccgtttgggc ggcgcaatac ggcttaggcg gcggcagagg
    17161 ggacaatgcc aactcggatt acagttattt cgcaaaactg tacgacccca aaatcctcgc
    17221 ttccaaccaa gccaaaatca cgatgctgat cgaaaaccgg tcgaaatata aatttgccta
    17281 ttggaacaat gtgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg
    17341 ctacgacaaa aacagcagca gcgcgaaaga cgatccgaaa tacaccaccg ccatccgggg
    17401 gcagattccc catttgggtt cggaacgcgc gcacgcgggc ttcagctacg gcacagggtt
    17461 cgactggcgg tttaccaagc atctgcactt gttggcaaaa tacagcaccg gcttccgcgc
    17521 accgacttcg gacgaaacct ggctgctgtt cccacacccc gatttctacc tgaaaaccaa
    17581 tcccgaactg aaagcggaaa aagccaaaaa ctgggaattg ggcctggcgg gcagcggcaa
    17641 agctggcagc ttcaagcttt cgggcttcaa aaccaaatac cgcgacttta tcgaattgac
    17701 gtatatgggc gtttcgtcag acgataaaaa caaccccaga tacgccccgc tttcagacgg
    17761 cacggcattg gtcagctcgc ccgtttggca aaaccaaaac cgcaccgccg cctgggtgaa
    17821 aggcatagag tttaacggca cttggaacct cgacagcatc gggctgccca aagggctgca
    17881 caccggcctc aacgtcagct acatcaaggg caaggcaacg caaaacaacg gcaaagaaac
    17941 gcccatcaac gcgctttcgc cgtggacggc ggtttacagc ctgggctatg acgcgccttc
    18001 caaacgctgg ggcgtcaacg cctacgccgc gcgcaccgcc gccaaaaagc cgtccgacac
    18061 cgtccacagc aacgacgact tgaacaaacc gtggccttat gccaaacaca gcaagaccta
    18121 tacgctgttc gacctttccg cctacctcaa catcggcaaa caggttacgc tccgcgccgc
    18181 cgcgtacaac attaccaaca agcagtacta cacttgggaa tctttacgca gcgtccgcga
    18241 gttcggcacg gtcaaccgcg tcaacaacaa aacccacgcc ggcatccaac gctttacctc
    18301 gccgggcagg agctacaatt tcaccatcga agcgaagttc taagctcctg atgtgccaat
    18361 gccgtctgaa acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc
    18421 ccctttcagg cttgtaccaa gcccaatccc gcaactctcc gtcattccca gggaagtggg
    18481 aatctagaaa cgcaaagcca caggaactta tccgaaacaa cagcaaccta aaattcgtca
    18541 ttcccacgaa agtgggaatc cagttcgttg agtttcagtt atttaaaata aattttgaaa
    18601 ctttaatccc atcattcccg cgaaagcggg aatccggaac gtaaaatcta aagaaaccgt
    18661 tttatccgat aagtttccgt accgacgggg ctggattccc gcctgcgcgg gaatgacggc
    18721 ggagcggttt ctgttttttc cgataaattc ctaaaactta aaatttcatc attcccacaa
    18781 ggacagaaaa ccaaaaacag aaacctaaaa ttcgtcattc ccgcaaaagc gggaatccag
    18841 ttcgttgagt ttcagttatt tagaataaat tttgaaactt taatcccgtc attcccgcga
    18901 aagcgggaat ccggaacgta aaatctgaag aaaccgtttt cccgataagt ttccgtgccg
    18961 acggggctgg attcccgcct gcgcgggaat gacgatattt cagttttctg tttttgattt
    19021 ttttgttttt acaggaatga cggggctttc agtattgaaa aaagttgccg taccgcgccg
    19081 ataatttccg cctgcgcgga atgaagattt aagcgttgcc cgaaattcaa aaaaccaaaa
    19141 ccgaacggat cggattcccg tttttatggg ataacggaat gttcagccgg acaccgccac
    19201 aatgcctccg gcccgcttat tttccgatgc aaaacctcga aaaaatcacg ccgagcaggt
    19261 cgtccgccgt aaattccccc gtaatttcgc cgcacgcgac ctgagccagc cgcaagtgtt
    19321 cggcaaacag ctcgatttga tggttgccgc acaaagcggc cagcgacaat tcttcctgcg
    19381 ccgccttgag tgcgttgacg tgccgcgtcc gcgccaaaaa cagcccttcg ctttcgccct
    19441 gccaaccggc ctcgcacaac agcgtccgtt tcagcgcgtc caagccgtcg ccggttttcg
    19501 ccgacagcgc gatgacggtt tccgcgcccg taccgaaccc gcctgccgcg tgtgcgtgca
    19561 aatcggattt gctgtggatt tcaatgcgtt tcaaatccga cggcaacgta tccaaaatca
    19621 tccgcgtctt ttcattcaaa ccctcgcgcg gatcgaccaa caccagcgcg acatcggctt
    19681 cggatacggc tttgcggctg cgttcgatgc cgatgcgttc gaccacgtcg tccgtctcgc
    19741 gcaaacctgc cgtatcgaca atatgcaccg gcacgccgtc aatcaggata cgttccctga
    19801 ccgcgtcgcg cgtcgttccg gcaatatcgg taacaatcgc cacttcgtcg cccgccagcg
    19861 cgttcaacag gctggacttg ccgacattcg gcgcaccgac caatacgaca ttcagacctt
    19921 cgcgtaaaat cgcgccctgc tgcgcgttgg caagcacatc atccacggcg cggcgcaagc
    19981 cgtccagttt gccgcgtgcg tccgccgctt cgagaaaatc gatgtcttcc tcgggaaaat
    20041 ccagcgccgc ttcgaccagc atccgcaagg taatcaagcc ttcgaccaaa ccgtgtatcc
    20101 gccgcgaaaa atcgcccttg agcgagcgca acgccagacg cgccgccgaa cggccggatg
    20161 cgtcaatcaa atccgccacg ccttccgcct gtgccaagtc cagtttgtcg tttaaaaacg
    20221 cacgtttggt aaactcgccc ggctcggcaa ggcgcgcgcc cagttccaaa cagcggttca
    20281 gcagcatctc catcactacc ggcccgccgt gtccctgaag ctcgatgaca tcttcgcccg
    20341 taaaacttgc cggcgcggca aaaaacagta gcagcccgct atcgattgcc tgtccgtccg
    20401 cgtccgtaaa atcagcatag gttgccacgc gcggctcggg cgttttccca cacaaagcct
    20461 gcgccatcgg caacaggttt ttccccgata tgcgtatcac gcccacgccg ccgcgtccgg
    20521 gcgcggtcgc gactgccgca atcgttggaa cgttatccga cataaaaccc ccgaaaattc
    20581 aaaacagccg cgattatagc aaatgccgtc tgaagtccga cggttcggct ttcagatggc
    20641 attgccggct tgtgtaaaac gatattccga caccgcacgg cacaaaaccc tgccccgcat
    20701 tccgcctccg gtcggttttt accgccgaaa acggcggggt tccgaccgaa aaggaacggg
    20761 gataaagtaa agtgtgatat gatttcgtat tcaaaatgat gcctttatgt gcaacccatc
    20821 gattgctccg gcgagttgat aaaaatcccc tttttttcct tcccgccctt ccctgcacca
    20881 tctccgaaca gaagcggcaa aatacgccga accgcacata tagccacatc ccgcgcgccg
    20941 cccggaagcg gaagccgcgc cctcccaaac aaaccgaatc ccgtcagata aggaaaaata
    21001 atgaaaacaa ccgacaaacg gacaaccgaa acacaccgca aagcccctaa aaccggccgc
    21061 atccgcttct cgcccgctta cttagccata tgcctgtcgt tcggcattct gccccaagcc
    21121 cgggcgggac acacttattt cggcatcaac taccaatact atcgcgactt tgccgaaaat
    21181 aaaggcaagt ttgcagtcgg ggcgaaagat attgaggttt acaacaaaaa aggggagttg
    21241 gtcggcaaat cgatgacgaa agccccgatg attgattttt ctgtggtatc gcgtaacggc
    21301 gtggcggcat tggcgggcga tcaatatatt gtgagcgtgg cacataacgg cggctataac
    21361 aatgttgatt ttggtgcgga gggaagcaat cccgatcagc accgcttttc ttaccaaatt
    21421 gtgaaaagaa ataattataa agcagggact aacggccatc cttatggcgg cgattatcat
    21481 atgccgcgtt tgcacaaatt tgtcacagat gcagaacctg ttgagatgac cagttatatg
    21541 gatgggtgga aatacgctga tttaaataaa taccctgatc gtgttcgaat cggagcaggc
    21601 agacaatatt ggcggtctga tgaagacgaa cccaataacc gcgaaagttc atatcatatt
    21661 gcaagcgcat attcttggct cgtcggtggc aatacctttg cacaaaatgg atcaggtggt
    21721 ggcacagtca acttaggtag cgaaaaaatt aaacatagcc catatggttt tttaccaaca
    21781 ggaggctcat ttggcgacag tggctcacca atgtttatct atgatgccca aaagcaaaag
    21841 tggttaatta atggggtatt gcaaacaggc aacccctata taggaaaaag caatggcttc
    21901 cagctagttc gtaaagattg gttctatgat gaaatctttg ctggagatac ccattcagta
    21961 ttctacgaac cacatcaaaa tgggaaatac ttttttaacg acaataataa tggcgcagga
    22021 aaaatcgatg ccaaacataa acactattct ctaccttata gattaaaaac acgaaccgtt
    22081 caattgttta atgtttcttt atccgagaca gcaagagaac ctgtttatca tgctgcaggt
    22141 ggggtcaaca gttatcgacc cagactgaat aatggagaaa atatttcctt tattgacaaa
    22201 ggaaaaggtg aattgatact taccagcaac atcaaccaag gcgcgggcgg tttgtatttt
    22261 gagggtaatt ttacggtctc gcctaaaaac aacgaaacgt ggcaaggcgc gggcgttcat
    22321 atcagtgatg gcagtaccgt tacttggaaa gtaaacggcg tggcaaacga ccgcctgtcc
    22381 aaaatcggca aaggcacgct gctggttcaa gccaaagggg aaaaccaagg ctcggtcagc
    22441 gtgggcgacg gtaaagtcat cttagatcag caggcggacg atcaaggcaa aaaacaagcc
    22501 tttagtgaaa tcggcttggt cagcggcagg gggacggtgc aactgaatgc cgataatcag
    22561 ttcaaccccg acaaactcta tttcggcttt cgcggcggac gtttggattt gaacgggcat
    22621 tcgctttcgt tccaccgcat tcaaaatacc gatgaagggg cgatgattgt caaccacaat
    22681 caagacaaag aatccaccgt taccattaca ggcaataaag atattactac aaccggcaat
    22741 aacaacaact tggatagcaa aaaagaaatt gcctacaacg gttggtttgg cgagaaagat
    22801 gcaaccaaaa cgaacgggcg gctcaatctg aattaccaac cggaagaagc ggatcgcact
    22861 ttactgcttt ccggcggaac aaatttaaac ggcaatatca cgcaaacaaa cggcaaactg
    22921 tttttcagcg gcagaccgac accgcacgcc tacaatcatt taggaagcgg gtggtcaaaa
    22981 atggaaggta tcccacaagg agaaatcgtg tgggacaacg attggatcga ccgcacattt
    23041 aaagcggaaa acttccatat tcagggcgga caagcggtgg tttcccgcaa tgttgccaaa
    23101 gtggaaggcg attggcattt aagcaatcac gcccaagcag ttttcggtgt cgcaccgcat
    23161 caaagccaca caatctgtac acgttcggac tggacgggtc tgacaagttg taccgaaaaa
    23221 accattaccg acgataaagt gattgcttca ttgagcaaga ccgacatcag aggcaatgtc
    23281 agccttgccg atcacgctca tttaaatctc acaggacttg ccacactcaa cggcaatctt
    23341 agtgcaggcg gagacacgca ctatacggtt acgcgcaacg ccacccaaaa cggcaacctc
    23401 agcctcgtgg gcaatgccca agcaacattt aatcaagcca cattaaacgg caacacatcg
    23461 gcttcggaca atgcttcatt taatctaagc aacaacgccg tacaaaacgg cagtctgacg
    23521 ctttccgaca acgctaaggc aaacgtaagc cattccgcac tcaacggcaa tgtctcccta
    23581 gccgataagg cagtattcca ttttgaaaac agccgcttta ccggaaaaat cagcggcggc
    23641 aaggatacgg cattacactt aaaagacagc gaatggacgc tgccgtcggg cacggaatta
    23701 ggcaatttaa accttgacaa cgccaccatt acactcaatt ccgcctatcg acacgatgcg
    23761 gcaggcgcgc aaaccggcag tgcggcagat gcgccgcgcc gccgttcgcg ccgttcccta
    23821 ttatccgtta cgccgccaac ttcggcagaa tcccgtttca acacgctgac ggtaaacggc
    23881 aaattgaacg gtcagggaac attccgcttt atgtcggaac tcttcggcta ccgcagcggc
    23941 aaattgaagc tggcggaaag ttccgaaggc acttacacct tggctgtcaa caataccggc
    24001 aacgaacccg taagtctcga gcaattgacg gtagtggaag gaaaagacaa cacaccgctg
    24061 tccgaaaatc ttaatttcac cctgcaaaac gaacacgtcg atgccggcgc atggcgttat
    24121 cagcttatcc gcaaagacgg cgagttccgc ctgcataatc cggtcaaaga acaagagctt
    24181 tccgacaaac tcggcaaggc gggagaaaca gaggccgcct tgacggcaaa acaggcacaa
    24241 cttgccgcca aacaacaggc ggaaaaagac aacgcgcaaa gccttgacgc gctgattgcg
    24301 gccgggcgcg atgccaccga aaaggcagaa agcgttgccg aaccggcccg gcaggcaggc
    24361 ggggaaaatg ccggcattat gcaggcggag gaagagaaaa aacgggtgca ggcggataaa
    24421 gacaccgcct tggcgaaaca gcgcgaagcg gaaacccggc cggctaccac cgccttcccc
    24481 cgcgcccgcc gcgcccgccg ggatttgccg caaccgcaac cccaaccgca gcgcgacctg
    24541 atcagccgtt atgccaatag cggtttgagt gaattttccg ccacgctcaa cagcgttttc
    24601 gccgtacagg acgaattgga ccgcgtgttt gccgaagacc gccgcaacgc cgtttggaca
    24661 agcggcatcc gggacaccaa acactaccgt tcgcaagatt tccgcgccta ccgccaacaa
    24721 accgacctgc gccaaatcgg tatgcagaaa aacctcggca gcgggcgcgt cggcatcctg
    24781 ttttcgcaca accggaccgg aaacaccttc gacgacggca tcggcaactc ggcacggctt
    24841 gcccacggtg ccgttttcgg gcaatacggc atcggcaggt tcgacatcgg catcagcgcg
    24901 ggcgcgggtt ttagtagcgg cagcctttca gacggcatca gaggcaaaat ccgccgccgc
    24961 gtgctgcatt acggcattca ggcaagatac cgcgcaggtt tcggcggatt cggcatcgaa
    25021 ccgcacatcg gcgcaacgcg ctatttcgtc caaaaagcgg attaccgata cgaaaacgtc
    25081 aatatcgcca ccccgggcct tgcattcaac cgctaccgcg cgggcattaa ggcagattat
    25141 tcattcaaac cggcgcaaca catttccatc acgccttatt tgagcctgtc ctataccgat
    25201 gccgcttccg gcaaagtccg aacgcgcgtc aataccgccg tattggcgca ggatttcggc
    25261 aaaacccgca gtgcggaatg gggcgtaaac gccgaaatca aaggtttcac gctgtccctc
    25321 cacgctgccg ccgccaaggg gccgcaattg gaagcgcagc acagcgcggg catcaaatta
    25381 ggctaccgct ggtaaccgcc ggatatgccg aaaggggttt gacgatgccg ccgtgcactg
    25441 tcaaacccct ttttctgcca ccttgtgcct actccatcgt ctgataaccg tcaaattgat
    25501 ttttagccca tgttcggcga gtcggggaaa tctatagtgg attaaattta aatcaggaca
    25561 aggcggcgaa gccgcagaca gtaaaaatag tacggcaagg cgaggcaacg ccgtaccggt
    25621 ttaaatttaa tccactatat atcttcgtcc gcgccgaaat agtctatgcc cgatatacaa
    25681 ttttgataca caaacttgga aatatcggta tcgtcgccgg tgcgatagaa tgcggatagt
    25741 ttttcattga atcgcggcat ctgcatttcg gagatttcca ggataccgca gccccccgcc
    25801 atcatcaggc cgttggcaaa tagggttgcc gtccgtttgt tgccgtccca aaaaacctgc
    25861 tgccgcatac aatagagcat gaaacgaacg gctgcttccg tcgtcgaacc gctttgcagt
    25921 ccgatatttt gcaacacgcg ggccacttca atttccttca ccggattcgg ggcatgacgg
    25981 gaaccgtcca atagcgttac gccgaccgaa ccggtacgga aatccacggg taccaaagaa
    26041 tcgtccttgg caacaatgtt gttgattctt ttaaggagtg aaatattgac cggttcgccg
    26101 tttgaaatat gcgaaatcac atattgatag gcacgtttca ggttcaagat ggtttggatg
    26161 tcttccagtg acgcggaggc tacatttttg ccattgataa tttgctcggt ctgcaacaag
    26221 gtcgtctgac aattctcaaa acggctgagg ttgtgaattt gagcaaccaa tactttcttt
    26281 gccagaaaaa tattttcttc caaactcaac ttgtatttgt cgggaaacac gataattcct
    26341 taaataatta aagcagtacg ttaaatcatc atcttatcat cactttcggg gtttatgttg
    26401 cgtgcgtccg ttttctaata cggtttccca tcccgcgaaa cgcggcggaa gcctgtgcaa
    26461 aaaacctgtt cacgaaattt tccgcataaa gatacgctga aaatgcgcta aaaatggctg
    26521 tgtttgaata tcggttgatt tcatccgttt gtgtaacctc gcgccggttt tgtcatgatt
    26581 ttgcattatt tttgttatgc ggatatttca cgggatgaca aaacgggcgc aaaaaagccc
    26641 gattggaaaa tccgaatcag gcttttttgt gatgttctgt tcgatttgtg cgctaactgc
    26701 cgaccggagc gtccgcgccc ggtttggcag gtcggattct cgaatccgac tgctatttga
    26761 gatggcaggc ttttgtcgga tacaagtatc cgacctacgg cttgctattg atttccaatc
    26821 attcgaatcg ggtattttac ttccttaaat tttgataaat caaaacgtaa cagttcaata
    26881 cttgtgcatt ctcaatgtgc ctcttcccaa ttctccccta cgccaacctc agccaccagc
    26941 ggtacatcca ataatccgcc gtccactttc gccataatct gcggcagttt ttctttgaca
    27001 aaatccagtt cggtttcaac gacttccagc accagttcgt catgcacctg catgaccagt
    27061 ttgctgccga tgccgtctga aagccagttt cgcacgttta tcatggcgcg tttgatgagg
    27121 tcggaagcgg tgccttgcat gggggcgttg atggcggcac gctcggctcc tgcgcgggcg
    27181 ttggcgtttt tgttgcggat gtcgggcaaa tagagccttc tgccgaacag ggtttcgacg
    27241 tagccttggg cggcggcttg ttctttggtg cgctgcatgt attcggcgac gccgggataa
    27301 cgggcgaagt agcggtcgat aaagtttttg gcggaaaggt tgtcgatgcc caatgatttt
    27361 gccaaaccgt attgccccat accgtagatt aagccgaagt tgatggtttt ggcgtagcgg
    27421 cgttgctcgg gcgagacgtt ttcgggcgca gtgccgaaca cttcggcggc ggtgcggcgg
    27481 tgtacgtctt cgccgctttg aaatgcggca atgagggttt tgtcaccgga gaggtgcgcc
    27541 ataatgcgca gctcgatttg ggaatagtcg gcggaaacga tgacgctgcc cggcggtgca
    27601 gtgaaggcgc ggcgcacgcg gcggccttcg gcggtgcgaa tggggatgtt ttgcaggttg
    27661 gggttgttgc tggcgaggcg gccggtaatg gcgacggctt gggcataggt ggtatgcacg
    27721 cggttatccc ggggcgaaat catttcgggc agtttgtcgg tgtaggtgga tttgagtttc
    27781 gccaggctgc ggttttgcag gatgattttg ggcagggggt agtcgggcgc gagctgttcg
    27841 agcacggctt cgttggtgga aatgccgcct ttggcggttt ttttcagccc ttttgtcggg
    27901 atgcccattt tgtcgaacag gatttcttgc agctgtttgg gcgagttgag gttaaacggt
    27961 tgacctgcag cggcgtaggc ttcctgttcg agcttcatca gctccgcgcc gagttccgcg
    28021 ctttggcggg cgagttcggc gcggtcgatt tgcacgccgt tgcattccat ttcaaacaat
    28081 acctgcgcga cgggaagctc cattttttca tacatttcaa gttgtttgtc gtccatttgc
    28141 gcgcgcaggc gcgcttcgag gcgcagggcg aaatcggcat cttgggcggc gtattcggtc
    28201 gcctgcccga tggcgacatc ggcaaaactg atttgcttcg cgcctttgcc gcacagcgat
    28261 tcgtaggtaa tggtttctaa accgagccag cgtccggaca attcgtccaa gccgtgtccg
    28321 agatggctct cgatgatgta ggaagcgagc atcgcgtcgc cggcaatgtt gtttaacgcg
    28381 atgccgtagt tggcgaaaac gtgttggtcg tatttgaggt tttgaccgat ttttttcagg
    28441 gcggggtttt ccaaatgcgg cttcagacgg cctaatacgt cttgcaaatc aagctgttcg
    28501 ggcgcggcag tcaggctgtg gcctacgggg atgtaaaccg cttcgcctgc ttggaacgca
    28561 atgctgatgc cgaccagctc ggcattcatc gcatctaatg acgtggtttc cgtatcaatg
    28621 ccgattgtgt ccgccttcgc tagtttgtcc aacaaggcgg caaactgagc ttcggtggta
    28681 acggcttgat aatccagttt ttcaggggcg gtggcttttt cagctcgttt ttcagacggt
    28741 atttccgcat tcaaagccgc ctgctcgccg atgctgtcgc taccgaacaa atcatcggtc
    28801 gagccggtat tcatgtttga ttccgcttct ttcagccaag tacggaagcc ccagcgtttg
    28861 aaatcaaccg caagctgcgc ccatttcggc gcggtacggc gcaggctttc gatgccgtct
    28921 gaaagctcgg tgtgcaagtc cacatcggtt ttaatcgtga ccaaatcata cgacagcggc
    28981 agttggggca gcgcggcttg caggttttcg ccgaccttgc ctttgatttc cggagcgtgt
    29041 tccatcacgc cttgcaacga accgtaggct tccagccatt tcaccgccgt tttcgggccg
    29101 catttttcca cgcccggtac gttgtccacc ttgtcgccga tcagcgcgag ataatcgcgg
    29161 atttggtcgg ggcgaacgcc gaattttgcc ttcacgcctt caatatccag cgtttctccg
    29221 ctcatcgtgt tcaccagcgt aacgcgctca tccaccaact gcgccatatc cttatcgccc
    29281 gtcgaaacaa tgactcgcaa accatgttcc gccccctgtt tcgccagcgt gccgatcaca
    29341 tcgtccgcct ccacttgccc aatcaccaat accggccagc ccgtcaggcg caccaaatcc
    29401 ggcagggctt ccgcctgcgg gcgcaaatcg tccggcatcg gcgggcgcgt cgccttgtat
    29461 tcttcaaaca tttgatggcg gaaatttttg cctttcgcat caaaaaccac cgcgcaataa
    29521 tcgtgcggat attccgaccg caaacggcgc aacatattca atacgccata cagcgcaccc
    29581 gtcggcgcac cgtcaggcgc agtcaggttt tgccccatcg cgtgatacgc gcggtagaga
    29641 taggaagagc cgtcaacgag gaggagtgta ggtctgttgg acataaaaaa cccgcttgaa
    29701 accgataagg gacagaaaaa atagggaagg acgatgccgg cttaaaaccc gcccccgtac
    29761 aaccccggat tataacggaa aaggcaaatg ccgtctgaaa cccttgttca gacagcattt
    29821 gcggcggatt aagcgttcag caggctttca tccaaagcca aatcgccgtt tcggttaacc
    29881 gccactttgc gcgccaacac gttttgcgcg atttgttgcg cttcggcgag cgagtgcatc
    29941 agataagtgc cgcattggta ttcgttcaac tcggggattt tactttggtc tttgacattc
    30001 aaaacatcct gcatcgaagc caaccacgca tcggcaacct gctgttcgga aggcgtgccg
    30061 ataaggctca tataaaaacc ggtgcggcag cccatcgggg aaatgtcgat gatttccacg
    30121 ccggcgccgt tcaagtggtc gcgcataaag ccggcaaaca aatgctccag cgtgtggatg
    30181 cctttttcgg gcaggatttc tttattgggg atgcaaaagc gcaggtcgaa tacagtaatg
    30241 gtgtcgcctt tgggcgtggt catggttttc gccacgcgta cggcaggggc gtgcatacgg
    30301 gtgtgatcga ctttgaaact gtctaacagg ggcatttggg tatccttttg tgcgggttat
    30361 gtaggttttc gggataggat tttgtccagc aattcatcgt ccaagacgcg gttttcgact
    30421 tttacccatt cgccgcctgc ttcagggaag ccgttgcggc aatatcctgc ctgttcgaca
    30481 aaatcataga cggcatggat gtagtcgttg atacacaatg cggctatggt gcaggcgcca
    30541 tcccataaaa tcaagacatg attggggata tgtttgtcgg atacatcttc gacattgtag
    30601 atgtgcaagg catccaaaat cccctcttgc gaagcggcat agaagtagcc cgtgttgcca
    30661 tcatcttcaa agacaacacc ataagggata tgtttggaaa atgattctaa aactttaggc
    30721 gtgccgacag taaaatttcc gatttcggca gtccgatata gaggtaattg tgccattggg
    30781 taacaatctt tttatatcga aaattgaatc acaattcggc aatctcaacc gcttcgcccg
    30841 aagccgcgct cagggcttcg tttaaaacgt cgtaaaaatc gcggctgtcg cgcgttgcca
    30901 gccatttgcc gttttgttcg gcgaaatggt agccgccgct ttttgcggca atccacaatt
    30961 cttggttggg cgtgtggcgg ttgacgatga tttgcgtgcc gtctccggct tcgatggtca
    31021 ggacgtttcc ggcaaaccgg cagtcgaaat cccagccgtt ttcgtcgatt tggtcttcga
    31081 tgtgttcaaa taatgcttcg ctcgcgcgga taaactcgct ttcggtcatc atagcttttt
    31141 gcgtgttttt tgtttaagat gccgaatctt gccacattcg cgcttatgaa ggaagtttac
    31201 cgatgaaata cggcgtattt tttgcggcgg caaccgccct cctgctttcc gcctgcggtt
    31261 acaaaggcgg cctctacctg cccaaagaag gcgatacggc gcgtttcggc gcaatccaaa
    31321 ccggtttgca gcttcaaggc aaaccgcaat ccgccccacc aacccaaaaa tgaaaacgaa
    31381 aatatgaccc tgttttgcga acaagtcgcc tacccccgcc ttgccgaaga attcggcacg
    31441 ccgctttatg tgtacagcca atccgcgctg accggagcat ttgaaaacta tcaaaccgcc
    31501 tttgccgctt tgaacccgct tgtctgctac gccgtcaagg caaacggcaa cctgagcatt
    31561 atcaaacact ttgcttcttt gggcagcggt tttgacattg tgtcgggcgg cgaattggca
    31621 cgcgttttgg cggcaggcgg cgatgcggcg aaaacgattt tttccggcgt aggcaaaagc
    31681 gaggcggaaa tcgagttcgc gctgaatgcc ggcgtaaaat gcttcaatat ggaaagcatc
    31741 cccgaaatcg accgcattca gaaaattgcc gcgcgtttgg gcaaaaccgc gcccgtctcc
    31801 ctgcgcgtca atcccgatgt cgatgcaaaa acccatccct acatctccac aggtctgaaa
    31861 gccaacaaat tcggcatcgc ctacgccgac gcgctcgaag cctaccgcca tgccgcacaa
    31921 cagcccaatt tgaaaatcat cggcatcgac tgccacatcg gttcgcaact gaccgactta
    31981 agcccactgg tcgaagcctg cgaacgcatt ttgattttgg ttgacgctct tgccgccgaa
    32041 ggcattgttt tggaacattt ggacttaggc ggcggcgtcg gcattgttta caaagacgaa
    32101 ggcgtccccg atttgggtgc gtatgcccga gcggttcaaa aactgatggg gacacgccgt
    32161 ctgaaactca ttcttgagcc aggccgcagc ttggtcggca acgcaggtgc attgctgacg
    32221 cgcgtcgaat ttgtcaaaca cggtgaagag aaaaactttg tgatggtcga tgcggcgatg
    32281 aacgatttga tgcgcccagc cctatacgat gcctaccacc acatcgaagc ggttgaaacc
    32341 aaaaacattg agcctctgac cgccaacatc gtcggcccga tttgtgaaac cggcgacttc
    32401 ctcggcaaag accgcaccat cgcctgcgaa gaaggcgatt tgctgcttat ccgcagcgcg
    32461 ggcgcatacg gggccagtat ggctagcaat tacaacacgc gcaaccgtgc ggcggaggtg
    32521 ttggttgacg gcggcggata caaactcatc cgccggcgcg aaaccttgga acagcaaatg
    32581 gcaaacgaac tcgcctgcct ataagccgaa catcaaaatg ccgtctgaag ccttcagacg
    32641 gcattgtccg tttaaagtga catcttggcg gaatattcaa acacagcctg ttttcaaatg
    32701 acgacgcaac aaatccccgt ctcgcccgac tgacgaaggg aattgttgaa aaaaattacg
    32761 cgcccctttt atggggtgca atatctaagg aattgaaatg aagcacgctc cgattatcct
    32821 cctgcttacc gcttcgtccg ccgccctact ttcggcgggc tgcaccgccc ttccctctga
    32881 ccggccgccg cttcgcaccc tgccgaaaag cgcgcccatc caacccgcca acccatacag
    32941 ccggccgcct tcaggcacgc cggacggcgc attcagcacc cgcgcctcaa acgggcgcgt
    33001 cttgaaaagc atcgtcaaaa acggcgtgtt cgaccgcttt gtcgatattt accatccaaa
    33061 cggcaaactg cattcgcaca cgccggttga aaacggcgtg gcgcaaggct ataccgaaca
    33121 gggcatattg cggacgcgca tcctgtaccg ggacgggcat atcgtccgag cgcaaacttt
    33181 ggatgcgtcg ggcaaggtgg aacgcgaatg gcagccgtaa aacaaatgcc gtctgaagcg
    33241 gttcagacgg catttttaac gttctaaagg cttactcttc cggcagcctt aacagggaaa
    33301 gcagcagccc gccccaaatc accgcgatgg cggcaaccat catcacaatg gcggaagtac
    33361 tcatttttct cctccttgtt catgttcgtg ttcgtctttg acattaaaat cctgaccgtg
    33421 tttccaaggc agcagcgaca gcagcagccc gaacatgatt aacgccgccg acatccccca
    33481 gccgaaaata ttgaggaaac catccggata atcgccgtag tttttttcca acaatccggc
    33541 ggtgtcctga tacagcatat agccgagcat cacgacggta aacacgacgc aggccgtcca
    33601 caagccgccg acgcggatgg aggacaaggc gttcaggtgc ctgcgtaatt ccggcaacct
    33661 gccgccgatg atgatggcgg caacataaac aaagccggcg gcaacaatgc cgtaggtgtt
    33721 gacgaatttg tccatcacgt ccaaaacagg cagccccgtc gccgtaccga acagcagcgt
    33781 ggaaacaatg cccatcggaa tgcagaccag cagcgtggcg ttgacgcgcc cgatgttcag
    33841 cttgtcctga atcgccgcca caatcacttc aaggatggaa atcatcgacg taacgccggc
    33901 gaacaccagc gaaccgaaaa acaatatgcc gatcagccag cccatcggcg cctggttgat
    33961 aatggtcgga aaggcgataa acgccaaacc gatgccgccg gaggcaacct cgttgaccgc
    34021 cttgccgccc gcctgcgcca taaagcccaa tgcggcaaac acgccgatgc ccgcgagcag
    34081 ttcgaagctg ctgttggcaa aaccgaccac cagccccgtt ccgcccaagt cggttttttt
    34141 cttcaaataa gaagaatagg taaccataat gccgaagcag atggaaagcg aaaagaaaat
    34201 ctgcccgtat gccgccaccc agaccttgga atcggcgagt ttcgaccagt cgggcgtaaa
    34261 caatgcgtcc aagccctttg ccgcacccgg cagcgtcagc gaaatgccga ccataatcaa
    34321 aaacatcacc aaaagcagcg gcataaagaa cgacgaggcg cgcgccacgc ccttttgcac
    34381 gcccaaagcc ataatggcgg cggtaaaaac ccacacgccc gccaaagggc cggcgacttt
    34441 gccgacaaaa tccaaaccca aggcttccgg gcccgccatt tgcaggaagt ccttaaagaa
    34501 aaaaccctgc ggatccgcac cccaggcggc gttgaccgaa taataggtat agcttgccgc
    34561 ccaaccgata atcactgcgt aatagatgca gatgacgata ttggtcatca cgttccacca
    34621 gccgaccggc tcgaaccagc gtccgaggcg gcggaaagcc aagggcgcag aaccccggta
    34681 acggtggccg atggcataat cgagcagcag cagcgggatg cccgccgtca gaagcgcgac
    34741 cagatagggc aggatgaacg cgccgccgcc gttttcaaaa gcaatatagg ggaaacgcca
    34801 aatattgccc aagccgacgg cggacccgat ggcggcaatc ataaacgcgc ggcgcgtgcc
    34861 gaatgtggcg cgttctttcg ttttggaatc agacacgttg atacctcttg aattatttat
    34921 taaaaacaag ccgttatagg caaacatata tagaaacagc gtctgacagg gataatgtct
    34981 tctgaaaaaa caaaccggct tgtggccgat tgtttacaat ctatgtgctt atcgtaaaaa
    35041 attttaacgc cgatggcaag cggtgaaggc ttgaaaacga aaaatttttt atccaaatac
    35101 aaaaaccact ccataagaaa caaccgccaa cttccccgtc attcccgcaa aaacagaaaa
    35161 acaaaaacag aaacctaaag tcccgtcatt cccgcgcagg cgggaatcca gacccccaac
    35221 gcggcaggaa tctatcggaa ataaccgaaa ccggacgaac ctagattccc gctttcgcgg
    35281 gaatgacggc ggaaaggttg ctgtttttcc gataaattcc tgccgctctt cgtttttggg
    35341 atggcgggaa atatagcgga ttaacaaaaa tcaggacaag gcggccagcc gcaggcagta
    35401 caaatggtac ggaaccggtt cgcccggtgc ttgggcgcct tagggaaccg ttccctttga
    35461 gccggggcgg ggcaacgccg taccggtttt tgttaatccg ctataatttt aaggtttgcg
    35521 ccttagccgc acttattcgc aaaaaaaccg cacggcgttg accgtgcggc ttgttgtctg
    35581 aaggtttcag acggcattgc ttacatcatg ccgcccatac cgcccattcc ccccatatcg
    35641 ggcacagccg gtttttcttc agggatttcg gcaatcatgc agtcggtcgt cagcatcaga
    35701 ccggcgatag acgcggcgtg ttgcagcgcg gaacgggtta ctttggcagg gtcgagtacg
    35761 cccattccga tcatgtcgcc gtattcgccg gagcctgcgt tgtaaccgta gttgcctttg
    35821 ccttccaaca ctttgttcac caccacgctg ggttctccgc ctgcgttggc aacgatttgg
    35881 cgcagcggag actcaacggc gcgcaatacg atttgtacgc ctgcgtcttg gtcggcattg
    35941 ccggtgtgca ggttttccaa agcggcacgg gcgcgcaaca gggctacgcc gccgcctgca
    36001 accacgcctt cttcaacggc tgcgcgggta gcgtgcagcg cgtcttccac gcggtctttt
    36061 ttctctttca tttcgacttc ggtcgccgcg ccgactttga tcactgccac gcctcctgcc
    36121 agtttggcaa cgcgctcttg cagtttttct ttgtcgtaat cgctggtcgc ggtttcgatt
    36181 tgttggcgga tttcggcaac acgcgcttcg atttgggctg cgtcgccgaa gccgtcgatg
    36241 acggtagtgt tttctttacc gatttcgatg cgtttggctt gacccaagtc gtccaaagtc
    36301 gctttttcca aagacaggcc gacttcttcg gaaatcacta cgccgccggt caggatggcg
    36361 atgtcttgca gcatcgcttt gcggcggtcg ccgaagccgg gggctttgac ggcaacggtt
    36421 ttcaggatgc cgcggatgtt gttcacgacc aaagtcgcca aggcttcgcc ttctacgtct
    36481 tcagcgataa tcaacagcgg acggctggct ttcgccactt gttccaacac gggcagcagg
    36541 tcgcggatgt tgctgatttt tttgtcgaac agcaaaacaa acggattgtc cagaccggcg
    36601 atttgttttt ccgcgtcgtt gatgaagtaa ggggacaggt agccgcggtc gaactgcata
    36661 ccttcaacca cgtccagctc gttttccaaa gatttgccgt cttcaacggt aatcacgcct
    36721 tctttgccga ctttttccat cgcttcggcg ataatcgcgc cgacttgttc gtcggagttg
    36781 gcggaaatcg agccgacttg ggcgatttct ttggaagtat cgcaaggttt ggcgatgttt
    36841 ttcagctcgt caaccaaagc ggcaacggct ttgtcgatgc cgcgtttcag atcggtcggg
    36901 ttcatgccgg cggtaacgta tttcatgcct tcggcaacga tggattgtgc caatacggtg
    36961 gcggtagtcg taccgtcgcc ggctacgtcg ttggttttgg acgcgacttc tttcaccatt
    37021 tgcgcgccca tattttcaaa cttgtctttc agttcgattt ctttggcgac ggttacgccg
    37081 tctttggtga tgtgcgggcc gccgaaagcg cggtcaacca ccacgttgcg gcctttgggg
    37141 cccaaggtta cgcgcacggc gtttgccaaa atattcacgc cgtttaccat tttttggcgg
    37201 acttcattgc cgaattgtac gtcttttgct gccatttcaa ttctccaaaa atcattaaaa
    37261 ctgtctgata aaaccgttta tgccgtctga aggcggtttg ccgtttcaga cggcatcgtg
    37321 tccgtattta tttttcaacg atgccgaaaa tatcttcttc gcgcattacc aacagctctt
    37381 cgccgtcggc ttttacggtt tggccgctgt atttgccgaa gatgattttg tcgccgactt
    37441 tgacatccag cggacggcgc gcgccgtctt tgccgatttt gcccgcgccc acggcgatga
    37501 cttcgcccat atcgggtttt tcggcggccg cacccggcag gacgatgccc gatgcggttt
    37561 tttcttcagc ttccaagcgt ttgacgacga cgcggtcgtg taaaggacgg atggtcatat
    37621 ttatgctccg ataaatggtt tgaaaacaat catctgcccg aacggttcgg acaaattgaa
    37681 gtggaaaccg ggctgccgcc taacggcctg cccgtacaag tcggcaaaat tagggtgtgt
    37741 gcgggaaaat tcaagtgagg cggaaaaaaa tttatttccg ccgtttttta tagtggacta
    37801 aatttaaacc ggtacggcgt tgcctcgcct tagctcaaag agaacgattc cctaaggtgc
    37861 tgaagcacca agtgaatcgg ttccgtacta tctgtactgc ctgcggcttc gccgccttgt
    37921 cctgattttt gttaatccac tatacatttc cgacaaaacc tgtcaacaaa aaacaacgct
    37981 tcgcaaataa aaacgataat cagctttaca caaccccccc ccccgctaat ataaacaaaa
    38041 ataattatta ttattttttc ttatcctgcc aaaccttaac ggtttggctt aacttccctt
    38101 catacactca aaaggacgaa caaatgaacg ccccgttttt ccgcctcagc ctgctctcgc
    38161 tcacacttgc cgccggcttt gcccacgcgg cagaaaataa tgccaatgtc gcattggata
    38221 ccgttaccgt aaaaggcgac cgccaaggca gcaaaatccg taccaacatc gttacgcttc
    38281 aacaaaaaga cgaaagcacc gcaaccgata tgcgcgaact cttaaaagaa gagccctcca
    38341 tcgatttcgg cggcggcaac ggcacgtccc aattcctgac gctgcgcggt atgggtcaga
    38401 actctgtcga catcaaggtg gacaacgcct attccgacag ccaaatcctt taccaccaag
    38461 gcagatttat tgtcgatccc gctttggtta aagtcgtttc cgtacagaaa ggcgcgggtt
    38521 ccgcctctgc cggtatcggc gcgaccaacg gcgcgatcat cgccaaaacc gtcgatgccc
    38581 aagacctgct caaaggcttg gataaaaact ggggcgtgcg cctcaacagc ggctttgccg
    38641 gcaacaacgg cgtaagctac ggcgcaagcg tattcggaaa agagggcaac ttcgacggtt
    38701 tgttctctta caaccgcaac gatgaaaaag attacgaagc cggcaaaggt ttccgcaatg
    38761 acaacggcgg caaaaccgta ccgtacagcg cgctggacaa acgcagctac ctcgccaaaa
    38821 tcggaacaac cttcggcgac ggcgaccacc gcatcgtatt gagccacatg aaagaccaac
    38881 accggggcat ccgcactgtg cgtgaagagt ttgccgtcag cgaaaaaaat tcacggataa
    38941 ctattaaacg ccaagcccca tcctaccgcg aaaccactca atccaacacc aacttggcgt
    39001 acaccggcaa agatttgggc tttgtcgaaa aactggatgc caacgcctat gtgttggaaa
    39061 agaaacgcta ttccgccgat gacaaagata acggctacgc aggcaatgta aaaggcccca
    39121 accatacccg aattaccact cggggtatga acttcaactt cgacagccgc cttgccgaac
    39181 aaaccctgtt gaaatacggc atcaactacc gccatcagga aatcaaaccg caagcgtttt
    39241 tgaacggcga atttaagatc tccgataagg aaaaaggacc caacggaaca gaagtcgacg
    39301 tagatgtagc acaaaaagca aaaaaccgtg caaatgaaaa aattgtttac gcctacaaac
    39361 tttccaaccc gaccaaaacc gataccggcg cgtatatcga agccattcac gagattgacg
    39421 gctttaccct gaccggcggg ctgcgttacg accgcttcaa ggtaaaaacc cacgacggca
    39481 aaaccgtttc aagcagcagc ctcaacccga gtttcggcgt gatttggcag ccgcgcgaac
    39541 actggaactt cagcgcgagc cacaactacg ccagccgcag cccgcgcctg tatgacgcgc
    39601 tgcaaaccca cggcaagcgc ggcatcatct cgattgccga cggcaccaaa gccgaacgcg
    39661 cgcgcaatac cgaaatcggc ttcaactaca acgacggcac gtttgccgca aacggcagct
    39721 acttccggca gaccatcaaa gacgcgcttg ccaatccgca aaaccgccac gactccgtcg
    39781 ccgtccgcga agccgtcaac accggctaca tcaaaaacca cggttacgaa ttgggcgcgt
    39841 cctaccgcac cggcggcctg accgccaaag tcggcgtaag ccgcagcaaa ccgcgctttt
    39901 acgataccca tcctaaaaaa ctgttgagcg cgaaccccga gtttggcgca caaaccggcc
    39961 gcacttggac ggcctccctt gcctaccgct tcaaaaaccc gaatctggaa atcggctggc
    40021 gcggacgcta tgttcaaaaa gctacgggtt cgatattggc ggcagggcaa aaagaccgcg
    40081 acggcaaatt ggaaaacgtt gtacgccaag gtttcggtgt gaacgacgtc ttcgccaact
    40141 ggaaaccatt gggcaaagac acgctcaatg tcaatctttc ggttaacaac gtgttcgaca
    40201 agttctacta tccgcacagc caacgctgga ccaataccct gccgggcgtg ggacgtgatg
    40261 tacgcctggg cgtgaactac aagttctaaa acgcacatcc cgaaaaaatg ccgtctgaaa
    40321 gcctttcaga cggcatctgt cctgataatt tgatatagat tatcatttat cctttctaaa
    40381 gccgttccgg tttgtccgac cggcggcttt gccccaatat ccccattttg gagacaccct
    40441 atgttgcgtt tgaccgcatt gaccctctgc accgccctcg ccttgggcgc gtgttcgccg
    40501 caaaattccg accctgcccc acaagccaaa gaacaggcgg tttccgccgc acaatccgaa
    40561 agcgcgtccg ttaccgtcaa aaccgcgcgc ggcgacgttc aaataccgca aaaccccgaa
    40621 cgcatcgccg tttacgattt gggtatgctc gacaccttga gcaaactggg cgtgaaaacc
    40681 ggtttgtccg tcgataaaaa ccgcctgccg tatttagagg aatatttcaa aacgaccaaa
    40741 cctgccggaa ctttgttcga gccggattac gaaacgctca atgcctacaa gccgcagctc
    40801 atcatcatcg gcagccgcgc agccaaggcg tttgacaaat tgaacgaaat cgcgccgacc
    40861 atcgagatga ccgccgatac cgccaatctc aaagaaagtg ccaaagagcg tatcgacgcg
    40921 ctggcgcaaa tcttcggcaa acaggcggaa gccgacaagc tgaaggcgga aatcgacgcg
    40981 tcttttgaag cggcgaaaac cgccgcgcaa ggcaaaggca aaggtttggt ggttttggtc
    41041 aacggcggca agatgtccgc cttcggcccg tcttcacgac tgggcggctg gctgcacaaa
    41101 gacatcggcg ttcccgctgt cgatgaatca attaaagaag gcagccacgg ccagcctatc
    41161 agcttcgaat acttgaaaga gaaaaatccc gactggctgt tcgtcctcga ccgcagcgcg
    41221 gccatcggcg aagagggtca ggcggcgaaa gacgtgttga acaatccgct ggttgccgaa
    41281 acgaccgcat ggaaaaaagg acaggtcgtt tacctcgttc ccgaaactta tttggcagcc
    41341 ggcggcgcgc aagagctgct gaacgcgagc aaacaggttg cggatgcttt taacgccgcg
    41401 aaataatgaa acggcggcat tcgatgccgt ctgaaacacg gatgcaaacc gcttcctgtg
    41461 tttcagacgg cattgcccga tacgggcgtt atcgcgggaa tggcggcaag cgttttgatt
    41521 ggaacttgcc ccatcctctg cccgtccgga ggtttcaaac aaggctttcc gctccgacgg
    41581 ttcggactgc cttgtttgaa tctcctacgc cgtaacgctt ttcccttctg tttatgactg
    41641 ccaaaccttt ttccctcaac ctggccaacc tcctgctgcc ggcggtgttg tttgccgtca
    41701 gcctgtcggt cggcattgcc gatttccgct ggtcggatgt gttttcgctg tccgacagcc
    41761 aacaggtcat gttcatcagc cgcctgccgc gcacgtttgc gattgtgttg acgggcgcgt
    41821 cgatggcggt ggcggggatg attatgcaga ttctgatgcg caaccgtttt gtcgagcctt
    41881 ctatggcggg tgcgggccaa agtgcggctt tgggtttgct tctgatgtcc ctgctgctgc
    41941 ctgccgcgcc gctgccggtc aaaatgtcgg ttgccgccgt tgccgcgctg atcgggatgt
    42001 tggtctttat gctgctgatc cgccgcctgc cgccgacggc gcagctgatg gtgccgctgg
    42061 tggggattat tttcggcggc gtggttgagg cggtggcgac gtttgtcgcg tatgagtttg
    42121 agatgctgca aatgttgggc gtgtggcagc agggcgactt ttcaagcgtg ctgctggggc
    42181 ggtacgagct gctttggatt acgggcggtt tggcggtgtt tgcctacctg attgccgacc
    42241 ggctgacgat tttggggctg ggcgagacgg tcagcgtgaa tttgggtttg aaccggacgg
    42301 cggtgttgtg gtcgggtttg attattgtgg cactgattac atcgctggtc attgtaacgg
    42361 tcggcaatat tccgtttatc gggctggtcg tgccgaatat cgtcagccgc ctgatgggcg
    42421 acaggctgcg ccaaagcctg cctgcggtcg ccctcttggg cgcgtctttg gttttattgt
    42481 gcgacattat cggacgcgtg attgtgtttc cgtttgaaat tccggtctct acggtttttg
    42541 gtgtgttggg tacggctttg tttttgtggc ttttattaag gaaacctgcc catgccgtct
    42601 gaaaaaaata tcggttttat ggcaggaagc agccgtccgt tgcgggtcgc ctttgcgctg
    42661 ttgctggttt cctgcatcct gtttatgacg ctcaacgtca aaggcgattg ggactttgtc
    42721 ttgcacctgc gcctgaccaa gcttgccgcg ctgctgatgg tcgcctatgc ggtcggcgtg
    42781 tccactcaac tcttccaaac gctgaccaac aacccgattc tgaccccttc gattttgggt
    42841 ttcgattcgc tgtatgtgtt tttgcagacc ttgctggtgt ttacgttcgg cggcgtgggc
    42901 tatacatccc tgccgttgac gggcaaattc gacttcgaac tggtcatcat gatgggcggc
    42961 tcgctgctgc tgttttacac gctcatccgt cagggcgggc gcgatttgcc gcgcatgatt
    43021 ttaatcggcg tgattttcgg gattttgttc cgcagccttt cctcgctgct ttcgcgcatg
    43081 atagaccccg aagaatttac cgccgcgcag gcgaatatgt ttgccggatt caataccgtc
    43141 cgcagcgagc ttttgggcat aggcgcgctg gtcctgctcg tcagcgcggc ggtcgtttgg
    43201 cacgaacgct accgctcgga cgtacacctt ttgaggcgcg accaagccgt caatttgggc
    43261 atcagctaca cgcgcaacac cttatggata ctgctttgga ttgccgcatt ggtggcgacg
    43321 gcgaccgccg ttgtcggccc ggtgagcttt ttcgggcttc tcgccgcctc gcttgccaac
    43381 cacttttccc cgtccgtgcg ccattccgtc cgcctgccga tgacggtttg cgtcggcggc
    43441 atcctcttgg tcggcggaca aaccgtattc gaacacttct tgggcatgaa ggcggtatta
    43501 agcgtggtgg tcgaatttgc gggcggactc gttttcctct atctcgtttt aaaacacaaa
    43561 aaatgacgga tgccgtctga acggcagccc gccccgaaag gacaaaccat atgacgcaag
    43621 aacgtttacc cgaatttttc gaccgcgccc cgacgctgac cgtacaagac ccgcttgccg
    43681 cattcctcgg cgcggccgaa aacggcatcc tcacttaccg ctacgccgat gccgtgcgcc
    43741 tgtgcggaca ttcctgcccg accgtcgcgg gcgcgtacct gatggttatc aaaggcctga
    43801 aagcacttta cggcgaagag ctgcccgaac gcggcggcat cgaagccgcc atgcagggaa
    43861 cgcgcgacga aggcacggtc ggcgtaaccg cgtccgtcgt ccaactcctt accggcgcag
    43921 cccccgaaac cggcttcgga ggcatcggaa tgcagggacg cttcgcccgc cgcaacctct
    43981 tatcctttgg tgcaggcgaa atcaacggca cactgaccct gcgccgcaaa gacagcggca
    44041 aaaccgtcgc cgtcagcctc aacgccgccc tgcaaccctt tgcacctcaa atgcgcgaca
    44101 tcatgcccaa agccgtcggc ggcagcgcaa gcgcaaaaga actcgaacgc ttcggacaac
    44161 tttggcaggc acgcgttaaa gcatttttga ccgaatcggc ggacgacccg cagttcgtca
    44221 tcgtccgcga actgtgaacg ttcagacggc attccgaatt tcaaatgccg tccgaacccc
    44281 cgccaaacaa caacaaacct acgcccgaca agcatccgcc atgattacca tccgcaacgt
    44341 cagctaccgc atcggcacac accccatcct cgacaacgtc agcctcgaca tccccgaagg
    44401 cggcattacc gcgctaatcg gccccaacgg tgcgggcaaa tccaccctgt tttcctttat
    44461 ggcgcggctg cgcccgcttg aaagcggcag catcgcctac cgaggcaaaa accttgccgg
    44521 cacccccacc gccgaactcg cccgcacgct ctccatcctc acccaagaaa acagcatcat
    44581 gagccgcatc accgtgcgcg acctgctgat gttcggccgt tacccctacc atcaaggcag
    44641 accgactgcc gaatgccgcc gtatcgttaa cggtgcaatc gaagaattcc acctgcagga
    44701 cctctccgac cgctacctga ccgaactctc cggcggacaa cgccaacgcg ccatgattgc
    44761 gatggtgttc tgccaaagca ccgactacgt ccttttggac gaaccgctga acaacctcga
    44821 tatgtatcac gcccgctcgc tcatgcagat tctgcaaaag ctcacgcacg aacacaaacg
    44881 caccaccgtc gccgtattgc acgacatcaa ccaggccgcc gcctacgccg accacgtcgt
    44941 cgccatgaaa aacggcaaag ccgccctgac cggcacaccc gaagaagtgt tcaccgtaca
    45001 caacatcaaa gaactgttcg acatggacgt ggacgtactc gactacgaag gtaaaaaact
    45061 catcgtccac cacatctgaa gctcaaaaag aaaggggctg tactagatta tccctaaatt
    45121 ccacaccgat cccgcaggat ttttagctgc cgggacggtg tgccggagtt aaatcgaaat
    45181 tctcattccc tcaacaacgg cgggaaaggt ttacg
//
LOCUS       DAXFWM010000009        43627 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000009 DAXFWM010000000
VERSION     DAXFWM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43627
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.009"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..466
                     /locus_tag="ACFI8B_000450"
     CDS             <1..466
                     /locus_tag="ACFI8B_000450"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3833743.1"
                     /translation="DMRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQ
                     SIALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWIL
                     ANSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            436..957
                     /locus_tag="ACFI8B_000451"
     CDS             436..957
                     /locus_tag="ACFI8B_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC3833744.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            1013..1309
                     /gene="traA"
                     /locus_tag="ACFI8B_000452"
     CDS             1013..1309
                     /gene="traA"
                     /locus_tag="ACFI8B_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HFC3833745.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            1331..1612
                     /gene="traL"
                     /locus_tag="ACFI8B_000453"
     CDS             1331..1612
                     /gene="traL"
                     /locus_tag="ACFI8B_000453"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFC3833746.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            1609..2274
                     /locus_tag="ACFI8B_000454"
     CDS             1609..2274
                     /locus_tag="ACFI8B_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFC3833747.1"
                     /translation="MKTEDMLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTI
                     VQTTLTPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSP
                     DYKDALERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQK
                     DRELLISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGN
                     RGN"
     gene            2276..3010
                     /locus_tag="ACFI8B_000455"
     CDS             2276..3010
                     /locus_tag="ACFI8B_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFC3833748.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMTSMMRGTTGD
                     MGINHSHEYQTIPLWKDTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            3012..4325
                     /locus_tag="ACFI8B_000456"
     CDS             3012..4325
                     /locus_tag="ACFI8B_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC3833749.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRSIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            4335..5054
                     /locus_tag="ACFI8B_000457"
     CDS             4335..5054
                     /locus_tag="ACFI8B_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC3833750.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            5051..5632
                     /locus_tag="ACFI8B_000458"
     CDS             5051..5632
                     /locus_tag="ACFI8B_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFC3833751.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            5642..8218
                     /gene="traC"
                     /locus_tag="ACFI8B_000459"
     CDS             5642..8218
                     /gene="traC"
                     /locus_tag="ACFI8B_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFC3833752.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            8246..8794
                     /locus_tag="ACFI8B_000460"
     CDS             8246..8794
                     /locus_tag="ACFI8B_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833753.1"
                     /translation="MTEAKEKKENTANTAPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            8794..9339
                     /locus_tag="ACFI8B_000461"
     CDS             8794..9339
                     /locus_tag="ACFI8B_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFC3833754.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            9348..10004
                     /locus_tag="ACFI8B_000462"
     CDS             9348..10004
                     /locus_tag="ACFI8B_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFC3833755.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPPVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            10001..11119
                     /locus_tag="ACFI8B_000463"
     CDS             10001..11119
                     /locus_tag="ACFI8B_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFC3833756.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTAPIPNKLTCQGKM
                     LNPINDICWSCMFPIKIGGKINLNMDNQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADTAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCMEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAAAGFPFNKLYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMA
                     ARIQTKMHRDLVAWRTSGRDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            11134..11886
                     /gene="trbC"
                     /locus_tag="ACFI8B_000464"
     CDS             11134..11886
                     /gene="trbC"
                     /locus_tag="ACFI8B_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFC3833757.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            11883..13127
                     /locus_tag="ACFI8B_000465"
     CDS             11883..13127
                     /locus_tag="ACFI8B_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833758.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTLLGSAERGESWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            13166..14707
                     /gene="traN"
                     /locus_tag="ACFI8B_000466"
     CDS             13166..14707
                     /gene="traN"
                     /locus_tag="ACFI8B_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFC3833759.1"
                     /translation="MLITSASAAALRESAACTRTSSVCVDGPSTKNINGVDVTKDCWE
                     YKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLRYTHKYSCDVDLRTL
                     HQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEPGSTKTINGVPVTRD
                     CWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTCQAREKQFECTEKGE
                     TTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQCSIKLDGALDSVFG
                     GDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFVSSAAQAMGTALSAA
                     GGITGTSQIGALGFSAAGQQGMGVIVGFNPAVFAAAIAVIAIQQWLKCPQSEILVAMK
                     RKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGKEQLKRGWGTPENPK
                     CEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKIREASVNGKNLEVKN
                     YYEYQ"
     gene            14694..15119
                     /locus_tag="ACFI8B_000467"
     CDS             14694..15119
                     /locus_tag="ACFI8B_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833760.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            15131..15907
                     /locus_tag="ACFI8B_000468"
     CDS             15131..15907
                     /locus_tag="ACFI8B_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFC3833761.1"
                     /translation="MAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWYPDAKK
                     PKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEVVMNKS
                     AAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGENGIMFV
                     FKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLNITTVP
                     AMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            16079..17590
                     /locus_tag="ACFI8B_000469"
     CDS             16079..17590
                     /locus_tag="ACFI8B_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFC3833762.1"
                     /translation="MKFHIFRFPKPAKVSAVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGTWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            17597..20599
                     /locus_tag="ACFI8B_000470"
     CDS             17597..20599
                     /locus_tag="ACFI8B_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFC3833763.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMHQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALAT
                     LGNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGDSRAQMRNSSFGVSSQSQISQSESYSKAADR
                     AETSAQQWQASFRESVSQGNANRLAFATNYGMDRSASEGYGMGLDAAESRKLSYVIDQ
                     GKKIAQKFGVTNTSAVISAIAAGLGISGNTGSSTKAQNIKNALGAKGDLGWSGKSQNT
                     DQMVAEIGESIEAGKRQGISFDSNFTDKVSQSEAYKVALNSGNSLAQTAEANFSKARE
                     AAHMSAESYQEAQSYREMAARTYGNGITIAQDNTNALVQDNPELTMSELNAGQGGTYN
                     AAIRAGVNSQDRLKAAAENSGWRPNDLSPEDRPQNNTQAAYKHFSDKVSSGAGAAFGD
                     VQTQAHQAGIIPDGINAAINARGENLKQEYANKSGKVEGDINQGRNSHRQEVGRLATH
                     AKNAQDETNGVTMRINTHALPWNSFGERYDNAMKDVREGKGYIGSGSDQAAAHLGNPA
                     LASEFDLPIPQRPGNSKSSGSAVFRPKE"
     gene            20605..21186
                     /locus_tag="ACFI8B_000471"
     CDS             20605..21186
                     /locus_tag="ACFI8B_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFC3833764.1"
                     /translation="MRNATSYGGNGVMWRGISSGRHVRGNYVLKASTAHNQAELERYV
                     HNPQVRAFLDFIAASEGVKHGYQTAIGNYRITDFSRHPGNSVRRSFTRTDGSRSTSDA
                     SGRYQFMGYTWKGLAKQYGFTDFSPATQDKAAVALILAKKGAMEAILNGDYEQAVMKL
                     GGIWASFPTAPNEYRQHKRSWGFVHNFFRQRGF"
     gene            21190..21348
                     /locus_tag="ACFI8B_000472"
     CDS             21190..21348
                     /locus_tag="ACFI8B_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833765.1"
                     /translation="MSIAQIVVQIEEVDEGNYQVRMSYSGGNDLVSEEGKSAFEQIIT
                     SIGKVLET"
     gene            complement(21405..21728)
                     /locus_tag="ACFI8B_000473"
     CDS             complement(21405..21728)
                     /locus_tag="ACFI8B_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833766.1"
                     /translation="MKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEVTLNLFVEGFW
                     IIGLLQAAVFTFVVDCSSSDLGSLSQWQIGNDWDRQMDPFYAHEIGNIWHGTHLDSSS
                     EEYWK"
     gene            complement(21844..22422)
                     /locus_tag="ACFI8B_000474"
     CDS             complement(21844..22422)
                     /locus_tag="ACFI8B_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC3833767.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVSNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNKLFRNMKPGSDADVVT
                     NNDWLNDFWEDRRRHCPSYSHHPSNIWHLSED"
     gene            complement(22626..22976)
                     /gene="exp2"
                     /locus_tag="ACFI8B_000475"
     CDS             complement(22626..22976)
                     /gene="exp2"
                     /locus_tag="ACFI8B_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Exp2"
                     /protein_id="HFC3833768.1"
                     /translation="MKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEATLNLFVEGFW
                     IIGLLQALAFTTIVDYCTESKHTNMLNNPACNDEDWDNDMLNNPACNDEDWDNDMLNN
                     PAYSWSMFNIHHSD"
     gene            complement(23043..23657)
                     /locus_tag="ACFI8B_000476"
     CDS             complement(23043..23657)
                     /locus_tag="ACFI8B_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833769.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQAYRSKVTDVQP
                     GKKISTSYGPALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            complement(23710..24795)
                     /locus_tag="ACFI8B_000477"
     CDS             complement(23710..24795)
                     /locus_tag="ACFI8B_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833770.1"
                     /translation="MSVYEPDKSKKGGGEKIALWRQTGYIGFYCCLILMMLFFPKDFN
                     RLKIIAYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYS
                     EADSGGASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSE
                     YRIDIDGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALS
                     KIGIPVVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRN
                     PLSADFQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQ
                     NFQAHSGKREIPKKHGILQRMASWFKS"
     gene            complement(24900..25349)
                     /locus_tag="ACFI8B_000478"
     CDS             complement(24900..25349)
                     /locus_tag="ACFI8B_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFC3833771.1"
                     /translation="MPDSPMQINGYTIYEHECFTRQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            complement(25351..25662)
                     /locus_tag="ACFI8B_000479"
     CDS             complement(25351..25662)
                     /locus_tag="ACFI8B_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFC3833772.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            complement(26066..26530)
                     /locus_tag="ACFI8B_000480"
     CDS             complement(26066..26530)
                     /locus_tag="ACFI8B_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833773.1"
                     /translation="MVENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLYTAVRLLNENQGILHGISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESKLDACLK"
     gene            complement(26619..26870)
                     /locus_tag="ACFI8B_000481"
     CDS             complement(26619..26870)
                     /locus_tag="ACFI8B_000481"
                     /inference="COORDINATES: protein motif:HMM:NF037233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833774.1"
                     /translation="MTVGESGIRISCDTAWGPPEGIYRELAKRFPDVEFEAKYLEEGM
                     WFAGTYEGHEGALFDYPCTDDGVRDFATEHFGCEYDDED"
     gene            complement(26915..27397)
                     /locus_tag="ACFI8B_000482"
     CDS             complement(26915..27397)
                     /locus_tag="ACFI8B_000482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833775.1"
                     /translation="MPNWCSNSLHFQGNPQRIAEVAEQILIKTDKGYQFSFQHIVPMP
                     EELILPEVPDSYMQQTWFQIAQQPEKKLINLEREMLEQRDKSFWSVQYRLPDELFSEL
                     KLYFSEKFDWDNLTVGEVFLHFEANSEVASRFRYDGPFAKRIAENIRHTVIRIGMTGG
                     "
     gene            complement(27710..27949)
                     /locus_tag="ACFI8B_000483"
     CDS             complement(27710..27949)
                     /locus_tag="ACFI8B_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833776.1"
                     /translation="MPDNMKSKTKVPTAVLASWAAILDDIAKAAIIGIGVVVWSNGYS
                     EEMKNLAMAGLVLLSFEMLYVSFLIRIHISKLEDK"
     gene            complement(28100..28615)
                     /locus_tag="ACFI8B_000484"
     CDS             complement(28100..28615)
                     /locus_tag="ACFI8B_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833777.1"
                     /translation="MSIKVDNILLIEGTKESVAEAAKLILFRSPVHPEHWAFDFNRIL
                     PLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIEICFLTGLAPPFGIYRKLAE
                     IFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMADIRKFSIAEFDIWYEGLSD
                     RLESDGYLVED"
     gene            complement(28712..28921)
                     /locus_tag="ACFI8B_000485"
     CDS             complement(28712..28921)
                     /locus_tag="ACFI8B_000485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833778.1"
                     /translation="MQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGFSLSDVLTD
                     GCRHVLREVVTGWLNREILPELYNV"
     gene            complement(28902..29282)
                     /locus_tag="ACFI8B_000486"
     CDS             complement(28902..29282)
                     /locus_tag="ACFI8B_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833779.1"
                     /translation="MQNFNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKRFGLG
                     ADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGKGVSAVADMRYLMP
                     AYRQYFNRPDKMVIFQGGIAVFIR"
     gene            complement(29518..29859)
                     /locus_tag="ACFI8B_000487"
     CDS             complement(29518..29859)
                     /locus_tag="ACFI8B_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833780.1"
                     /translation="MEQLDSAAPAACDTPSLFDLDRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            complement(29938..31059)
                     /locus_tag="ACFI8B_000488"
     CDS             complement(29938..31059)
                     /locus_tag="ACFI8B_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HFC3833781.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTNGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            complement(31908..32165)
                     /locus_tag="ACFI8B_000489"
     CDS             complement(31908..32165)
                     /locus_tag="ACFI8B_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833782.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKFLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            complement(32413..34896)
                     /locus_tag="ACFI8B_000490"
     CDS             complement(32413..34896)
                     /locus_tag="ACFI8B_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFC3833783.1"
                     /translation="MYNPVSKIDELLPDFAGLDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALDRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEGSVEVQLAKRFVDRQD"
     gene            complement(34992..35483)
                     /locus_tag="ACFI8B_000491"
     CDS             complement(34992..35483)
                     /locus_tag="ACFI8B_000491"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3833784.1"
                     /translation="MPLPDFRIRLLDAADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRISKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            complement(35485..35769)
                     /locus_tag="ACFI8B_000492"
     CDS             complement(35485..35769)
                     /locus_tag="ACFI8B_000492"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFC3833785.1"
                     /translation="MFMTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAR
                     EAAQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            complement(36002..36343)
                     /locus_tag="ACFI8B_000493"
     CDS             complement(36002..36343)
                     /locus_tag="ACFI8B_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833786.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARISSFEKSVDARFSAIDTKINAIYWFIGIT
                     TTVAVAAAKFL"
     gene            complement(36569..37150)
                     /locus_tag="ACFI8B_000494"
     CDS             complement(36569..37150)
                     /locus_tag="ACFI8B_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833787.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            complement(37160..37675)
                     /locus_tag="ACFI8B_000495"
     CDS             complement(37160..37675)
                     /locus_tag="ACFI8B_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFC3833788.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKNPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            38516..38839
                     /locus_tag="ACFI8B_000496"
     CDS             38516..38839
                     /locus_tag="ACFI8B_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833789.1"
                     /translation="MTYGREGHSGKLPKGQPSNSCFKSNKNKKFGLLKNCIFLLMLTI
                     DMSIDKKTILLKAKSLRGLTLCFTIIQELLGKLNRRFDRGLIFQAMSWKTKNGEARKL
                     PTILK"
     gene            complement(38860..39309)
                     /locus_tag="ACFI8B_000497"
     CDS             complement(38860..39309)
                     /locus_tag="ACFI8B_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFC3833790.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            complement(39573..40145)
                     /locus_tag="ACFI8B_000498"
     CDS             complement(39573..40145)
                     /locus_tag="ACFI8B_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC3833791.1"
                     /translation="MDRRNFLKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDR
                     TINCKRADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYA
                     MQEWARQSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELE
                     QMAKYYNVGGIGIYNSFVHLDTGRVRHWRG"
     gene            complement(40485..40787)
                     /locus_tag="ACFI8B_000499"
     CDS             complement(40485..40787)
                     /locus_tag="ACFI8B_000499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833792.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGSCFYCG"
     gene            complement(40802..42841)
                     /locus_tag="ACFI8B_000500"
     CDS             complement(40802..42841)
                     /locus_tag="ACFI8B_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC3833793.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            complement(43033..43464)
                     /locus_tag="ACFI8B_000501"
     CDS             complement(43033..43464)
                     /locus_tag="ACFI8B_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3833794.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
BASE COUNT        12421 a         9721 c        10041 g        11444 t
ORIGIN      
        1 agacatgaga ttaagggcca tacttttaca gttcgtcgta ccgggtgttt tggtactcac
       61 atctgcaaga gcagttgctt tttgttataa ggaagccggc agtaaatatg gcatcgatcc
      121 cgccatgttg caatcgatcg cattaaccga gagcgctttc aggccgaata ttgaaagtca
      181 tacagctgat attggcttga tgggtatcaa ccgttcatgg ctacccgtac tccacaagaa
      241 atttggttta acaggggcgg atgtatggaa tccatgcaca aatgtacata ttggtgcatg
      301 gatattggca aatagttatc ggcaacatgg gaaatcatgg gaagcagttg gtgcttataa
      361 cgccgcatgt accaaactta aaggccgggc gtgctatcgc gcaagaatga cttacgcaaa
      421 taaggtatat caaaattgga aacgattaaa aacacgctct tcgtagcctc cgcaatgatt
      481 ctgacagcct gtgccgcccc aaaacaggaa gtggccgggc tgcctcaaga cgcaacttat
      541 acagtggaac aaaatcagct gggcgaattt gtcttctgcc gcaatgaaga gtgttcggga
      601 tttgagcgca aggttttggc ggaacaaccg gtttatgctc aaacacaaac caatcttcaa
      661 aaagatgaga tcagggtgtt tttcaattta ggcagttcgc acctcggtgc ggagggcgtg
      721 agaacgctca aacgtgttct gcctaaattg aaaaatactc agacggttta tttacgcgga
      781 tgggccgaca gtatcggagg tcgaaatact gcaattaata gacgtttggc taaacaacgg
      841 gcagcagtaa taggtaaatg gctgaggaaa cataatgtat ccgcaaaaat aagaacagca
      901 agttccccgg cctgctgcaa tagtgatgat acgagaaccg ttgtaatcac ttggtaaaga
      961 atgatcggac gcaaaagctt ggcgatataa gctctgttta atgaggttca taatgaatat
     1021 caaacgcaat aaattagtaa agtccgctag tgcggttgca ttggcattgt atgctaactt
     1081 ggcaatggcc gctacaacag gtgccgaatt caaaggcttg gctgacatgg taacaggctg
     1141 gtctgaaggt tatctgggta tggctttggg cttgactgca ttcttgatcg gtttggcggt
     1201 aggcctgatg aaacaaacca tcatgccatg tatcgtcggt ttgggtgttg gcttggccgc
     1261 aacattaggc ccgggcatca ttaaaggtat gtttaccttg attatttaat caaactagat
     1321 aagagaagtg atgagagatt atcatgtgcc ggtatcgata gacgatccca aaaaggcatt
     1381 tatttgggac ttggacgttt ttatattatt tatgtccggc ttcggtatag gtattgtctc
     1441 tcgtcacttt ttcatttcca tgtcaattag ccttttcatt gcatggaggt ggggcaagct
     1501 taagtctggc aagcatcaat ggtttttttt gcatgttctt tactggtact tacccattaa
     1561 tgaaaaaaac aaacggatac ctgaaacgaa taaacgggag tttctaaatt gaagactgaa
     1621 gatatgttga gtgaattggc gataagaacc ggcataaagg tctttttttg gatacttttg
     1681 ggaatgtcta tagctgtcaa tctatttttg tccgcgaccc tgctcttcaa aggtacgatt
     1741 gttcaaacga cgctgacccc gccggaaatc cggcggtcaa tgactgtatc aaacatcgct
     1801 ttttctaaag aatatcttga agaaatggct ccctatgctg cctatcttct cttaaatgta
     1861 acccctaaaa cagtcgatta tcagaatagt caattattga agattgtttc acctgattat
     1921 aaagatgccc tagagaggga gctgtcttta aatgccttat gggttaagaa aaataatgtc
     1981 ggcaccactt ttagcgcgac agaagcaacg gcggacattt cagacaatac agtttccatt
     2041 cgtggggttt ttgaggttaa acgcaacaat gccctggtag agcaaaaaga ccgcgagcta
     2101 cttatttctt tcaaaaataa ttatggaaca ctgcaattat tatcgattaa agaagttcaa
     2161 aagcgtgtaa ctaaagcaga acccgcaggt caggaaaaat ccgaagttga aaccgaggat
     2221 gtagaaatca gtaaaacggc aacagaattt aaaggcggta atcgaggtaa ttgatatgtg
     2281 ttcactgtta aaaaaaattg tcttttctgc cttgttggca gccggacccg cgactagttt
     2341 tgcagcgcaa cgagtgccgg caacagaaaa tacacctcac gttgtttcaa taagtaaacg
     2401 gaacttgagc agaatcgcca ttgaaggcgg tcgaatttca tcatggaaat ttatggaagg
     2461 tgatcttgag cttcagaaag atacaacaac aggccaactg tttgtccgta gtttgacctc
     2521 caatccgaca aatcttttcg ttataagcga agaaggtaag acctatttgc tggttcttaa
     2581 acctacatca aaacagggtg acaacatcgt aatcgatgtg gctggagcta atcgccgtga
     2641 agcagcagta ttggcttcgc aaaccggtcc aatgcctgta acgatgaata gtacagaata
     2701 tgtacgggcc atcaaaaaaa tgatgacctc tatgatgcgt ggcactaccg gcgatatggg
     2761 catcaatcat agtcatgaat atcaaaccat cccattatgg aaagacaccc tctttattca
     2821 aaacggaagt tacaccgccg ctgatatgca aggcctttct tttacattaa ctaatttagg
     2881 tactaaacaa cttgaaatta gagagcagga attttaccga caaggagttt tggcggtagc
     2941 ggttcggaaa cagatcctac aacccgggga aattaccgac gtatttatta tcagccgtcc
     3001 gggaggttga gatgagggtg aaagtaaaca aatttaaact ttcagcagcg cgaaaacgcc
     3061 agttagccat cgcgggcatg gttgcgggca gcatggcggc gattgtgggc agtgcaattt
     3121 atgtcattga aggcaataaa aagaaatctg ccgaagtcac acaggttcaa aacaaacggc
     3181 agattctcag aactgatttt acaagccccc aagcaggcat tacggacaat tccttatgga
     3241 tgaacacaga aagttcgaag attgaatatg caaatcgaaa aatcagcgaa ttggaaacca
     3301 tggttcagga gttaaaagaa aaagagaaca gcagtaatcc tgatacaagt aaggggctgg
     3361 ggcctgatgg attaggcaaa ccgcctgcaa ttagcggtat cggggataac ggccggcttc
     3421 ctccggcccc tccggcaggc acattgccga acggggcgcc tccggcagat aggtccattg
     3481 aacggaaaat cgtttcagga agtatgagtg aagcagagct gcaacctgga acgaccggat
     3541 cggtaaatac cggaaatccg aatgaaaatg tacgggtttc cccgcaatta aaagaagcgg
     3601 agtgggttaa atcaaaaacc ccgcgtatga atatagaagt agttgaagac ggcggtaagg
     3661 ttgtcgtctc gcaaggcaag tttcgggcgc gtgactcgta tatacctagc ggtacttttt
     3721 tccgatccgt actgttaggt ggggttgacg caccgaccgg gggtgaggca caaaatgcca
     3781 gcccccaccc tgttttaatg cgcgtaacgg attttgcgca acttcccaac cggtttaaat
     3841 acaatttcag agaatgtttc gtaaccgggc aggcttacgg cgatatatct tcagaacgcg
     3901 cttacatcag actgcagaat ctcagctgtg taggtaccga tggccgagcc atcgacatgc
     3961 cggtaaaagg ttacgttgcc ggtgaagacg gcaaaacagg cgtgagaggt aatcttgtaa
     4021 cgaaacaagg ccaactcttg gcaaatgcac tgatgtccgg cgtgattagc ggtatgggta
     4081 agggcgtatc ggaagcattc aaggtgacga ataataccgc atttggcagc acaacctcta
     4141 ttcggggcag tgatcaatat cgtgcaggga ttgcatctgg tatcggtggt gcagctgaca
     4201 ggcttgctga gtattacata aaactggccg ataaggtctt cccggtagtt gaagtcaatg
     4261 caggccgtca agtcgatgtc gtactgactc aaggtatcga aattgatacc ggcgaaacca
     4321 aataggatcc atctatgaaa aagaaaatct tggccactat tgcagcaatc acagcttttc
     4381 agacggcctt tgccgatgtc aaaactgttg gaagcactct gcacaagttg tatccgaata
     4441 ccacgttttc ttcagtaaag gctaccccca tggcttcaat ttatgaagtg acaatgggtg
     4501 acaacattgc ctatgtacaa gaaaacggtc gctatttcat ttttggcgcg ttgtacgata
     4561 tgcaagagca gaaagacttg accgagatgg cccgttcagc ggtaacacaa aagagttatt
     4621 cccgactgcc attcaaaaac gccatcaaaa tcgttaaagg taatggcggc aaaggcaagc
     4681 gagaatttgc acttttttcc gacccggatt gcccgttctg tcgtcgtttg gaagaaacat
     4741 tggccggcat gacagactat accgcatacg ttttcatgtt cccgattaaa tccctccacc
     4801 ctgatgcaat ttcaaaagca gaacatatct ggtgcagtaa agatcgtgaa aaagcatgga
     4861 ataactacat gctgatggat aaagaaccgg cggccggtaa ctgtaaaaac cctgtatcag
     4921 aaaatattgc actcgcagag cagcttaaag ttcgcggtac tccaagcatg attcataaag
     4981 atggtcggcg tacatccggt gccatgccgc gtgccgaatt ggaaaaatgg ttaaatggag
     5041 cgggagcaga atgatgcgtt tgaaagcctt atcgattact gcggcaatat tgattcttag
     5101 cggatgttca accttaacca tgtccggtat cggcggtagt gaaaaattcc gatgccctaa
     5161 tgcccggaac agtgacgatc cttattgtga atctataagc agtaactata aagcatctgt
     5221 tgcaggtgtt ttaaaagacg gtatgaaaag acaggttgga caaccttaca ctgatcaaag
     5281 tgttcgcaca ttaatgttaa cgcaagctta ttcctccggt acgcccgttc gctcgcaaag
     5341 tgaaattgct cgcatttggg tcgctcccta tcttgatacg gacggcgatt taaatgacca
     5401 aagtttcaca tatgtcgttc tgaatcaggg tgattggtta attgcccaca acgggcaaca
     5461 gattataaat gaatatcgtc ccatccgctt attgggggat ggggccaaaa cttcaggcag
     5521 ttcggttgag acttctccaa ccaattcaaa caacacaaat gaccgcatcc ccgatgttgg
     5581 cgtgaatctt catctgcagg aaacatcggg tattcccggt accgttcgat agaggtatgt
     5641 aatgggcgtc ctgtcaaaat ttaaaaattg gttggccggt tcggatgatg tcgatcaacc
     5701 gatggaagcc ttgcacaaca accctgtcga tcctgacttt gaccatttga gcgactatct
     5761 caattacggt gcatacgata ccaaaacaga gttgtttacg ctgatgtccg ttaaaggcga
     5821 caaaccgttg ggcatgggct ttattctgga acttaaccca tttttaggcg gcagtgacga
     5881 cctgatcggt aagtggggca gcctgtatgc gctgcttcct gaaaagacgc cgatacaggt
     5941 tcaaattttc ggctcccccg atttaagaaa ttacttcaag gaatatgagc aagtacaagc
     6001 atcacgacct atagacgatc cgatgcgaag cactttcgaa actttggcca aaaaacgtac
     6061 tgaattttgg aataagggta cacagaaggt cttggtagaa aatacggcca ttcgtttacg
     6121 aaatctgcgc tgtatcctca gcgtaaattt gaatattcca cctgataatg cagtggaagt
     6181 cagcaaggcg gtttcgctcc ttgcccgcat gcgcgattca ctccgctcca tgcagattta
     6241 cggccgaact tggaatgcag acgatttatt ggcttggaca acgctcctac tcaaccctaa
     6301 taggatgttt accggacaac aagatgaaat cgatcctgtt tgggatgaaa ccaagttctt
     6361 gagtgaacaa atgattgaga cccgcacatc tatcaaggtt cttgatagcg gctcggggct
     6421 gcgttttggc aaccgtgccg cgggggattg cgtgattgct caatgctatt ctgccaatcg
     6481 ctaccctgaa gagtttcatc tgtccaatat gggcgcattg attggtgatg tcattgaggc
     6541 aaacatgaat tacacatcac catttctgat ttccatggcg atgttcaagc gtgattatga
     6601 cacttccagc aataccgtaa aactgaaagc ggcccgcgcc aaacaaacgg cggaatccaa
     6661 gatggccgca gttatgccgg aggcagcaaa aatcaaacgt gattacgaca tatgcctaga
     6721 agcgttcggc aaaggcggcg gcggtctggt gagtttgttg caccaagttg tcatttggga
     6781 gcgtcccgaa aacattaact tggctgaaag ccaggcggta tcgatttggc aggctcaggg
     6841 cttcggtctt tatcgtgacc aatatcttca actggggtca tatttgactg ccctcccaat
     6901 ggctatcgac aaagaggttg agaaatattt ggacagtaag aaaagatggt caaccaaaac
     6961 catgactaat gctgtctgta tgtctcccgt tatcggcgag tggcacggtt taggaagacc
     7021 tgtcatcggt ttgttcggaa aacgcggtca ggccatggga atcgacttat ttgccaaccc
     7081 ggcaggtaac tataactttg ccattattgg cgcttccggt tccggcaaat cattttttgc
     7141 caatgaaatt gtgcgcaact acatggggct ggggactcag gtatggatta tcgacgttgg
     7201 ccgttcgtat gaaaaattct gccaaatgat tggcgggcag tatattgaat ttacctatga
     7261 agcaggcctg tgcttcccgc cattccaaat ggtaacggac atcaacgaag atattgagat
     7321 gttgaaacta atcttctcat tgatggcctc tccgtcagac tccctaacgg aacagcagga
     7381 agccaagctt caacggataa tcgaagaagt atggtacgaa ttcggacaag aaggcacggt
     7441 cgatcatgta agggaacgct gtctcaagtg tttgattacc ggcagtaatg aaggaggaaa
     7501 gcccggtgaa cgagatttgt ctatggtggc cgttgccgac caattgtaca catactgttc
     7561 ggaaggtatc tacggcagtt acttcaatgg aaaattgaat gtcgagttta aaaatgacct
     7621 ggtcgtactg gaacttgaag agttaaaaac caaaccggat ctgcaacaga tcatcatgca
     7681 gctgattatg taccgcatta tgcagggcat gtacctatca cgaagccagt acaaaattat
     7741 gatgattgac gaggcttggg ctttgttggg cgacacgggt acagccgctc gttttattga
     7801 ggaaggctac cgccgtgtcc gtaagtacaa aggtgcatgc ggcactctga cgcaaggtgc
     7861 aaatgactac tacaagacta aggcggctga ggcggcatta gagaatgccg actttgtttt
     7921 ccaattgcgt accggggcta aatcgctcaa aaccattcaa gacaaagaac cttttgaagt
     7981 caatgacggg attatgcaga ttctgcgaac attgagcaaa actgactact acagtgaaat
     8041 atttgtctct accccgatag gttatggagt aggtaggttg tactcggacc cattcaacgt
     8101 tcttgcctca agctcaaaag cggaagacgt tgaagccgtg aaccattaca gaaatcaggg
     8161 attgaataca gccgaggcca ttgaagcagt tttaagggac cgtgccgcaa cccgctaatt
     8221 aggcagagga taggatatta ataagatgac tgaagcaaaa gaaaagaaag aaaacacggc
     8281 aaataccgct ccggaaaaga atccggagga taacggcgta gaggtttcat tgggcagcct
     8341 tgtgatcggc gcaatttcta ttgccgcatt ggcattaggc gtatggaatt tcatgaaaac
     8401 ccatgagaaa ccggtccata ttgtagattt ggccggtatc tccatgactt accgggaaca
     8461 ggcgaagaat caaggactgc agaatggaat ttccgaccgg caacgagccg cgatcctcca
     8521 aaactaccaa gcaaagatga ataccttgca acaggttatc gaagaaaagg taattcggtg
     8581 cggctgtaac atctttgtca aatctgcatt ggtcggacgg aacacagagg tggttgatgt
     8641 tactgctgac attatgaaag agttggataa gcgtgtcccg gccacagctg tagtggcggc
     8701 accggtacat caacaagttg aaaagacaat tccgaaaccg atggccgaag agggtaaagg
     8761 caccccggtt actgaagaag aaatggatcg ttaatgtttg aggcaatgat aaaaatatca
     8821 caaaaagaaa gcaacaaagc agtgatgaaa cgtctttata gggagttttg tcagaaatat
     8881 gcaacccctt ttttagcagt tgcggtctgc ttgtcggcat tcaatcaaca ttatgctttg
     8941 gcatttaatc tttcccgttc cttgcctcac catctttact tcatcaaaaa agatgcgaac
     9001 aagctaagcg atctgaagca gggcgattat gtggcctttg catggcaagg tggtttttac
     9061 cctattggta cgcaggtcgt caaagaggtt gccggacttc ccggaaatca tgtaacgaag
     9121 gcgaatcgta cttttttcat agatgggaaa gaggtaggta ctgcaaaaga atactccctg
     9181 gatggcatga agctggccat caacccattc gaaggggaaa ttccacaacg gttcatgtgg
     9241 gttaaaacag ggcacaaaga cagcttggac agtcgatatg aattatccgg cttaatccat
     9301 tcagggcaaa ttattggtaa ggcggtcccc attttttagg agtgagtatg tcccacaaga
     9361 aaaaaatagc ttcagtcgca tcgctagtaa tacttgcaac cggaatttcc tcatgggtag
     9421 ccgcttcgac accccctgtc gagaaggttg gcgacaaaat cggcaccaca tacccaatag
     9481 cggaaaaaca tgcaatccgc gaaattgaag cgcgtttgaa agctaaacaa gacagcggcc
     9541 aattggcggc aatgcagaaa gaggtccaag atagaataaa tcgctccgcc ttaaatctac
     9601 agcccttgga aggactggcg aaggcaacta aaggctctgt tcgatacttc gacccaagtt
     9661 ataccttaac cgaaacaatt tatgaccaag acggtcaaat tattgctcct gccggtactg
     9721 ttgtcaaacc tttggaagca gccccaatcc cattcaaaat gtttttcttt gacggccggg
     9781 aacctgacca aatcgaactg gccaagaaac ttgcagcaga atatggcgaa tcattcatgc
     9841 ctattctgac tgcaggcaat tggtatgagt tatcagtaga aatggatcag gcggtttatt
     9901 acgaccaaga agggcggatg tccaaaagtt ttcagctgaa tgaagttcca agtttggtct
     9961 cccaagaagg gggccggctc cgtgtagagg tcatgaaacc gtgaaaatat ttgtcacacc
    10021 gctgaaatta ctgattccgt tattactact cccattattt tctatatctc acgccgctga
    10081 agctgttcct actgctccga taccgaataa attaacctgc caggggaaga tgcttaatcc
    10141 gataaatgat atttgttgga gctgtatgtt cccaatcaaa atcggcggca aaatcaatct
    10201 gaatatggat aatcaagagg ataacggctc ttctccaaac aattggtttt gttcgtgtac
    10261 gcacccgagc cgttcaggcg tgacggtatc attttgggaa cccacacact tggtagaagt
    10321 cgtacgcacc cccttctgta tggttagcct aggcggtatt tcacttaaaa atcaaaaagc
    10381 agatacggcg gaaacaacag gtaaagcaga cgtatccaat aagattcaaa atctttttgg
    10441 cggctcgcat aagcgtggtc gtaacgaaca acatgatcag cataatgcct tttatcaagt
    10501 tcattggtat aaaaatccgg tcgtattttg gttggaagcc ttattggaca acgattgtat
    10561 ggaaaaaggt actttggata tcggttggat gacggaactt gatcccactt ggaaatatcc
    10621 gtcattggcc gtttatcaag cccctgattc tgcgctcttt gccaatgtgg ttgcacaagc
    10681 agcctgtgcg gctgactgtg ttaaagcggc cgccggcttt ccattcaata aattgtattg
    10741 gtgtgcaggt tgccacggaa cgatttaccc tttgtcagga tggatgtccg gccacttctc
    10801 cgaagtgcag gccgctaaat tgatggcggc tagaattcag acaaaaatgc acagggactt
    10861 agtagcttgg aggacatcag gcagggatgc tttatgcggg tacaaactta atcccgtaat
    10921 ggataaacag aactataaaa tggctatgac ctatccggtg gccgcccgaa aattcagagg
    10981 ccgttgctgt gagcctattg gacgctcaac attagtcaga gacagcggca aagcatttcc
    11041 atacaaaggt aatcagtttg ctttccagct tttccgtaag cgcgactgct gcggtgcata
    11101 taaatttaag actaattgac aggagatgga gtgatggaca agtctttacg caatgtcatc
    11161 gtagccggag gcgtcttcct cattcttttg ggcggtttgg ccggagtagg cagtgcttac
    11221 gccgatgcgg atgtagaggc ggcgcgaaac cgtctgcgta caaccatggg taaagttacc
    11281 cgagttaaaa acccaaatgt gggtgacaat cggcagcaac ctgaaattcc ggatttgaaa
    11341 ggtatcaagc accgtgtgcc cgatgttcgt gattacggaa atgcaaaaat caaggctgat
    11401 ccgatgtcta tcgcacaaaa ataccgcact gcttcacaag aagcccgaca ggctgcggaa
    11461 aaagcggatt taatggtatt cgtatcattt tcaatgcccg aagccagctt gaaacgcatt
    11521 gcccgtgaaa cggcaaaggc aggcggggta atggttatcc gcgggtttaa agacgactca
    11581 ctgaaagcca cggttaaggc ggccgaagaa cttgcggctt tgcaaggcga tatgctgatt
    11641 catcccgaac tcttcgacca ttacggcatc accgaagtcc ctgttacggt aatcgcccga
    11701 aacagcgaag atggtttaga cggttgcgct gtaaattcag aattggggat gtgtacggaa
    11761 catgttgccg taaagggtga tgtgtccttg cattcggctt tggaacactt gatccgtgag
    11821 tctgaagaca aaaaactttc cgatattgca aatgcgaaat tagccgttct ggaaggcaga
    11881 aaatgaaaag acatgccatt tatctaactg tatttggcct catgcagacg gcctttgctg
    11941 cagattacgg tagttacgtc ggcgaactgg gaaaattggg ggccgggact gcaacaaaca
    12001 tgatgcagcc tgcccaatat accccaagat atacagacaa cgtaccaatg gctgaacagt
    12061 attacggtgg cggagtttct cttccgactc agtttggtga cgccaaattc aacgactgta
    12121 aaaatctgcc tgccaatccg gatctctatc ttcgtcaaga atgcgaaggt gtaaacctga
    12181 ttgcaggaag ccgtaccaag cggcccgatg taaccatatc ttcaaatgaa aagttggtta
    12241 aaggcacgca gaaaattgca ggcgatccgg cagaaacact ggaaaaatac aaatggaaat
    12301 atcccgtcaa tgaagacggc agcgtcggca cagttccctc gtcggcatgt cctacggaaa
    12361 ccattaccgt tcctgcggta aaaaccgtca aacgttgcaa tgaataccat ggcgcagaga
    12421 tgttcttgtg cgaggcggcc cttaaggtca atgtcgatcc cggctggaac tattcctgcc
    12481 tggagagcaa atatcgaaac cagcagtatc aatgcggcaa gaaattaaag gtggtatgcg
    12541 agaaatcagg accaaactgt acccgttcgg gtatagaagc aggcagcctg cagggtgata
    12601 tgacggtcag gctgacggct tcgggaagcg cacacaggct gatgttcggt actattggcg
    12661 acaactattg gcgtgacggg caatatgatc gggagatgac gctcaatgta aaaaatccgg
    12721 aaaaactgac gctgtttact ttgagccggg ttgagtacga tgattggctg attgtcaaag
    12781 tcaacggcaa aattgtgtac agcagctata acaatcagat gtttgcggca gattatactg
    12841 caaaagatga agatggattt cctataggaa gaaggtttgc accggtacgt aatgaggaag
    12901 gtactctgct gggtagcgcg gaaaggggcg aatcctggag gaaaaacctg aacatcgaca
    12961 tacggccgta tctgcatcag ggcaaaaata ccatttggac ccgcaccgtt gtcggaggcg
    13021 gcggagaaaa tgctatcttc tttaatgtac accaatactg cgagcccgtt tgtcacgata
    13081 aatgggaaaa cagttgttcg gagtatgaga agagggtaaa acaatgaggg ctataacgtc
    13141 tttgctggtt atgatctgcc actttatgtt aatcacttca gcgtctgctg ccgcactcag
    13201 ggaaagtgca gcgtgtaccc ggacgtcttc cgtttgtgtt gatggcccgt cgaccaaaaa
    13261 catcaacggg gtcgacgtaa ctaaagactg ttgggaatac aaagaagagt atcagtgcct
    13321 tgagaaggat tctgccgatt attgcgcacc gttaaaagac ccttcggcaa aatgtgaagt
    13381 acagggacaa acctgtttgg agcagtcgaa tgagggggag tgtttgcgtt acacgcacaa
    13441 gtattcttgc gatgtggatt tgagaacgtt acatcaagga aggcttccga ccaaagtcga
    13501 agaaatggag catacccatc tgattagttc gcaatgggat gagtcgtcat gtcaggtaca
    13561 aggaaaaaaa tgtaaagcag ttgcaacaga gtgtttggaa cccggcagta ccaagaccat
    13621 caacggcgta cccgttacac gtgattgctg gaaagaaagg cgcaccgtgc aatgtacgga
    13681 cggaagcgat tccgaaacat gcagcgctta tacatcctca gaccaatgcc gtctgattgg
    13741 ggacaagtgt acgcatcagc tgccggacgg tacctgtcaa gccagagaaa aacagtttga
    13801 atgtaccgaa aaaggagaga caaccaaaga ggtatccggt tgtcaggatc gggattttgc
    13861 caaaactatg acgacgatgg aattcgcccg tgagacgcaa cggttttatg atcccgaaaa
    13921 acagcgtttt ttcaatggag aagccgggca atgctctatc aagctggatg gtgctttaga
    13981 cagcgtattc ggcggagact gctgtcgaac aaaagcagat ccgggcaaat ttgttgactt
    14041 cgcggtacag acagggacaa ctatggcaac aacgtatttt atggccagcg ttgccagcca
    14101 ttatacgttt accactatgt ttgtcagctc cgcagcacag gcaatgggta cggccctgag
    14161 tgcggccggc ggtattaccg gaacatctca gattggtgcg ttgggtttct ctgctgcggg
    14221 acaacagggt atgggggtaa ttgtcggctt caacccggcc gtatttgcgg ctgctattgc
    14281 cgtaatcgcc atccaacagt ggttgaagtg cccgcaaagt gaaattttag ttgccatgaa
    14341 gcgtaaagca gatttgtgcc attatgtcgg ttcttattgc ggcagcaaaa tattgggggc
    14401 atgtgtaaca atgatagaga gccagtgctg tttcatttcc aaattagcca aaatagtcaa
    14461 tgtcggcggc aaagagcaat tgaaacgggg ttggggtacg ccggagaacc cgaaatgcga
    14521 aggatttacc gcacaggaat tggaacagtt agacttttcc aaacttgatc tgtccgggtt
    14581 ttatgaagaa atctatgcca acatggacaa cgtggccaag caggggcaaa aggtttcaca
    14641 aaaaatcagg gaggcatccg ttaatgggaa aaatttggaa gtgaagaact attatgaata
    14701 tcaataattg gttggctgcg tcggcgggta ttgtatcggg tatgtttttt acaggtatgg
    14761 cacttgccgc atcccttcct tataccggcg gtatcgcttt ggaaaaaaca gaagctctag
    14821 caaggacttc catcccgctt cctgacggca agccggatgc cctgcttgcg ctggcaatgc
    14881 gtcacggttc ggcaaaaggg tatttcagcg gccgtgctgc ggctgccgtc aaacagaaat
    14941 tccggcagga catacctatt tttgtcgagg cggtcagaat ggaagaaatt aaaaacaaac
    15001 ccggatgcaa cagggttcgc ttgacattcc atacttcgcc cgcactgtcg caaacctttc
    15061 cgtctgaaat tatggatatc ggggcgtgcc cgaacagaaa gaacggtggt aaaaaatgaa
    15121 aagagtgcgg atggccgtca tgctgatatt tagcctgcac gcctccaatc tgcgagctgc
    15181 ggatgatggc atgggatggt atgaccaaga tatctggaaa aacgaaaacc gtgctttctt
    15241 atggtatccg gatgctaaaa agcccaaaca agccggacaa aaccggcctc aaaatactcc
    15301 ggttcgtaat gaacttgcag aatttgaaat gctgcaaaga aatttggatg agtcgcgtaa
    15361 aattgccgtc atgaatccca cacctcaaaa tttgaagcga tatattgaac ttcaagaggt
    15421 tgtgatgaac aaatctgcgg catttgccga tcaatggcaa agggtaattt ggcaaaaccc
    15481 aaatttggat tacagccagc gcggtcgtcc gaacaaccaa ctggcccagc aacaatatga
    15541 tcataaccgt cagcaacaaa aggccggggc aattagaaaa cttgccggag agaacggcat
    15601 tatgtttgtt ttcaaatcgg attgccctta ctgtcatacc atggcgccga ttttgaaaaa
    15661 ctttgccgac caatacggta ttaaggttat gccggtcagt ctcgatggcg gaggtattaa
    15721 tcacttcccg cgtgctttgc cgaacaacgg tattgcccaa aggttgaata ttactactgt
    15781 acctgcaatg tacgtcatgg atacaaaaac caagcaattt acacccattg gatttggggt
    15841 gatggcgcaa accacgcttg aggatcgttt cttagcgtat tcccgccctg tcgggacatt
    15901 gtattaatca aatcaaaggc cctatacggg gccttcaata tttatatttc tatgtattaa
    15961 ccggttgaca acgcggatat ttcaggtaaa ataagattcc cttaatctca agtctttact
    16021 gtaacttatc ttggttatag taaggatttc cctgctataa ccgcttcatg gagcggttat
    16081 gaaatttcac atctttcgat ttccgaagcc ggccaaagtg tcggcagttt gtgcttcctc
    16141 tgcaaaatat ctatgcttgg cggcatcgct agcccttccg atcaatagta gtgccggcat
    16201 agagaagaac atggctgaca tgtttaactc aatgggagcg gaagccaatt atactgaagc
    16261 aggtgcgttc cacggtcaaa gcggcagcct atatacggga ggcggtatga gcgttcgctt
    16321 tccggtgtcg gatatcagcc ttgggaatat tcaattacct tcaatcagtg ccggttgcgg
    16381 cggtatagat ttctttgccg gctctttctc gtttgtcaat aaagaacaat tcatccaatt
    16441 tacgcgaaac ctgggcaata acgcagcagg tgtagccttt gacctggcac ttaaggccct
    16501 tgatccgatg atccaagatg caattggcgg tattcgtgat ttggtcaatt atgttaatca
    16561 aaacagttta aacagttgca aactgagtca ggatattgtc ggcggcgcaa tgggaaaact
    16621 gggttcgttg gccagttcaa attgtcgcgc cgcatcagtt gattcgggtt cgtcaaatga
    16681 cccggcagaa ggtacttggt actgtcaggc cgctaaaaac cttgttcatg aagccgataa
    16741 ggtgcgaaac accggaaaac cccaagatac gattagcttt accggaggta atttgactta
    16801 tgaggccatg aagaactata cgggtcaacg ctcgaaactg ggtatcgaca tggacttcta
    16861 ttacagcatg ctgggaacaa ttgtgttcac tccattaaca caacatgaag gggataagga
    16921 caaatacaca gtaggtattc aaagtttcga accaaaaatc ctcagcatcc aagatctttt
    16981 aaacggaaaa aacaatgccg ggggagaaat ccgggagaag gttgtattgg atatgtggac
    17041 gtgcaaagac ggttctaaga aattaatgga gaactgcact gagaaaaatg atgtagaaat
    17101 accttctttg cggtacatgg ttcacaaacg cattgaaacg cttccgactg caataaggaa
    17161 taaccaaggt tggaatttat ctacgcgcaa gcagattgct gccattgtca ataacagtaa
    17221 attgcccata ctgaaaatgg ctgtatccga cgcatttctt ggcacgggct atctgaaaaa
    17281 aacagcggtt ttagatgcga ttgccgttga tattatggcc aatattttgg accaatcgga
    17341 acgccaggta cgctcgacct tgggtatgta taacaagaca gacgatgcca gcggggaagc
    17401 ggttcgaaaa ttgtttgcca accttggcga attgcgaagc agaatttacc aggagcgcat
    17461 tgactcaatg aagaaaatcg agatcgaaga aagcatgatg agggctatgg agcagtttga
    17521 aacacaatgg cgctcagcct ttgtcgatac caacaactcc atgacttttg attcgatgaa
    17581 ccgtttctaa ggagagatga tggctgtcga atactttact ttccctggta acggagaagc
    17641 tgtacgggcg gttttcaatg gcgttgccac aatatcagga tctacgatga tgggtggcgc
    17701 aatgcaggct gctgccttat ttggattcct ggtcactctt gctgttgcag tcttcaaatt
    17761 ggatttgaag gataacttca gttatttgtt tgtcgtgtta ttttgttgga tgggtatgat
    17821 ggtccctaaa accactgtac tcattactga atcaggtggg tatggatata caggccgtca
    17881 atatactgtc ggcaatgtac cagtcggcct tgcttatatg ggctactttg tcagttcttt
    17941 tggccaaagt attacccgca aagccgagca ggttaaccac ctgccggacg acctcaatta
    18001 ctcacgcacc ggcatgctgt tcggaacgcg cctgatggaa aatatccgtg aagcacgaat
    18061 cccggacgcg caactgactc aagactgggc actgttcatg catcagtgtt cattctttga
    18121 tatgaacctg tatcacttct ataacgtgca ggatctggca cagagtgccg atattctggc
    18181 tacattgacc cataccaacc aagccttgtt taccaatgta tcgcagatcg cctcaagaag
    18241 aggcaacatg ttgcagtata acggcaaatc aaagaccatg acctgcaatg aggcggcaaa
    18301 agaactaaaa gaccgtaccc ggttttatag caaaaattac ttgcccggct acattgccga
    18361 tcgtgttttt gcaggtttgg gtacaaattc ggcaggaata aaccgggtaa atgccctggc
    18421 cacgttaggc aatagctcat tccaatatct gctggataat gcccgcttcg atacattgaa
    18481 aaatatcgaa caggcggcga tggttgaggt tatccgtcaa gcaggcatta tcaatggcca
    18541 acgcaaccga aatccggctg ccgtacagca ggcatttgct caagttcaag ctcgtaatca
    18601 gtatattgca gcccaaaaaa ccggctcatc aatggcaagt tggaatcttc ccttgattcg
    18661 ttctgctgct gaggccattc tgattggttt attccccttt gtaatgattc tcgccatgtt
    18721 gggtggcatc atggcattcc ggttattaag cttctacatg atgagtatgc tatggattca
    18781 gctttgggca cccgtagcca gtatcatcaa tatgattatg actatgaatg cgaagcgtct
    18841 gtttagcgtg gaagccgcca acggcgttat tacaccggga acaggtgata cgttattgat
    18901 ggcagcagcc gatgcacaag cagctgctgg ggctgcaatg tggctgattc cggtcatcgc
    18961 gggtgctctt gccatgggcg gacgcagcct gatgaacggc atgatgggca tgacttcatc
    19021 ggccaaatca accggtgaag cagcgggttc ccaagtaggg tcggggaatt actctgccgg
    19081 caatatgaac tacaacaata gcaatgccaa caaacatagc ttaaaccctg tatataccga
    19141 tcctcaaatg atgactgctc aatctgcagc cggaacatca tggcggaata tggcaactgg
    19201 agatagtaga gcgcagatga ggaatagtag ttttggtgtt tcctctcaat cacagattag
    19261 ccaatcagag tcatattcga aggctgcaga ccgagcagaa acatctgccc aacaatggca
    19321 agcttccttt cgagagtccg tatctcaagg caatgcaaac cgccttgcct ttgcaaccaa
    19381 ttatggtatg gatagaagtg cctctgaagg ttacggaatg ggtttggatg cagctgaaag
    19441 tagaaaatta tcttacgtta tagatcaagg caagaagatt gcccaaaaat ttggagttac
    19501 taatacatca gcagttatat ctgccatcgc agctgggtta ggcattagtg gcaatacggg
    19561 cagtagtact aaagcccaaa atattaaaaa tgctttagga gcaaaaggtg atttaggttg
    19621 gagtggtaaa tcacaaaata cggaccaaat ggtagcagaa attggggaaa gtattgaagc
    19681 aggtaaacgc caaggtatta gctttgatag taattttaca gacaaagttt cccaatcaga
    19741 agcatataaa gttgcactca attctggtaa tagtttggca caaactgccg aagcaaactt
    19801 tagcaaagca agagaagccg cgcatatgtc agcagaaagc taccaagaag ctcaatctta
    19861 tcgagagatg gcagctcgca catatggtaa tggcattacc attgcccaag ataacacaaa
    19921 tgcattggta caagacaacc ccgaattgac tatgagtgaa ttgaatgcag ggcagggagg
    19981 aacctacaat gctgcaattc gtgcaggtgt aaatagtcaa gatcgtttaa aagctgctgc
    20041 ggaaaattca ggctggagac ctaatgacct ttcccctgaa gatagacctc aaaacaatac
    20101 gcaagccgct tataaacact tctctgataa agtttcatcc ggagctgggg cggcatttgg
    20161 agatgttcaa acccaagctc atcaggccgg tataattccg gatggaatta atgctgctat
    20221 caacgctagg ggtgagaatc tgaaacagga atatgcaaat aaatccggaa aggttgaagg
    20281 cgatattaat caaggtagaa atagccaccg ccaagaagtt ggtagattgg caacgcatgc
    20341 taaaaatgct caagatgaaa cgaatggggt aaccatgaga attaatactc acgccttacc
    20401 ctggaactct ttcggtgaaa gatacgataa tgcaatgaaa gacgttcgtg aaggaaaagg
    20461 atacataggt tctgggtcgg atcaagcagc agcccatttg ggcaacccgg ctttagcatc
    20521 cgaatttgac ctaccaattc ctcaacgtcc agggaattcg aagtctagcg gatccgctgt
    20581 attccgccca aaggaataac cccgttgcgt aatgccacct catatggagg caacggggtt
    20641 atgtggcgtg gaatatcaag tggaagacat gtgagaggaa actatgtatt aaaagcttca
    20701 actgctcata atcaagccga attggagcga tatgttcata atccgcaagt gcgcgccttt
    20761 ttagatttta ttgcggcatc agaaggtgta aaacatggtt atcagacggc aataggtaat
    20821 tatagaatta cggacttctc ccgtcacccg ggtaactctg ttagacgtag ttttactcga
    20881 accgatggta gtagaagcac atctgatgcc tccggccgct atcaattcat gggatacaca
    20941 tggaaaggac tggcaaaaca atatggcttc acggatttta gtcccgccac acaagacaaa
    21001 gctgctgtcg ccttaatttt agctaaaaaa ggtgcaatgg aagctatttt aaatggcgat
    21061 tacgagcagg cagtcatgaa attaggtggt atctgggcca gcttcccaac agctcctaat
    21121 gaataccggc aacacaaacg aagttggggt tttgtacata atttctttag gcagagagga
    21181 ttttaaaaga tgagtattgc acaaattgtt gttcagattg aagaggttga tgaagggaat
    21241 tatcaagtta gaatgagtta tagtggaggt aacgatctag tatccgaaga aggcaaatca
    21301 gctttcgaac aaatcatcac ttcaattggt aaggtactag aaacatagat aatttaacaa
    21361 tcttttggaa ataaaaaaac aggagtttat aaatctcctg ttttttattt ccaatactcc
    21421 tcagaactcg agtctaagtg cgttccatgc caaatgttac caatttcatg agcgtaaaat
    21481 ggatccattt gtctgtccca gtcatttcca atttgccatt gacttaatga acctaaatca
    21541 ctacttgagc agtctaccac aaacgtaaat actgctgctt ggagaaggcc aattatccaa
    21601 aaaccctcaa caaaaagatt taatgtcact tcgcctgtgc tgaagtacat taccgcaggt
    21661 gtagagatgg agaatgccgc tacaaataag ataacaagcc tcaaaagttt tttagatttt
    21721 gttttcaatt tgcactccta tgattggcag catttcagct accaatcctc tttcttatat
    21781 atcgtcattt gatcgatgaa atatattgca attgagattg gaaaaagctg ggttataaat
    21841 taatcaatcc tcagataaat gccaaatatt gctcggatga tgtgaatagg aaggacagtg
    21901 ccgacgccta tcctcccaga aatcgtttaa ccaatcatta ttggtgacaa catcagcatc
    21961 agaccccggt ttcatattcc tgaataactt attcagaaag aatgtcagca cggaaaaagt
    22021 taccgcaggg aagacaaaca gcaaaactat gttagtctcc ctatcagtat caaaaaagcc
    22081 ggctaagatg tagaaaaagc ccagtgttat gaacatagtc aaacacagcg ataatatagc
    22141 tgccgaaagc aagttgtatt ttttagacat gggttgcttt tcaactttct gaaagtcttt
    22201 gcataaggtt ttcaattttt ggatctgttg aggatcagga ttatgtatat tactaacctt
    22261 tcgcgctcgc agcccacgac tatgctcaaa tggttcgaaa ttcactatct gtcctggtat
    22321 atctgaaccg gcggatttac ggaaaaagta ttcatgacca tcttcaccaa agataaagcc
    22381 aaaatctttc tcatagctat accattttac gaatccgatc atgatacttc tcctttattt
    22441 ataggttact tgaatactat cacttgtaat ccaacgttca ttgcctctgc ttggctgaat
    22501 tttaattaag ccattctcac ctttttctaa gaccaaggca ggcccatagg aagtggagat
    22561 ttttttaccg ggttgtacat ccgtaacttt gctgcggaaa gcaggagtat aaggctcctg
    22621 cttttttaat ctgaatggtg aatattgaac attgaccaag agtacgctgg attattgagc
    22681 atatcattat cccaatcttc atcgttgcac gctggattat tgagcatatc attatcccaa
    22741 tcttcatcgt tgcacgctgg attattgagc atatttgtat gtttagattc agtgcaatag
    22801 tctacaatgg tggtaaaagc gagtgcttga agtaatccaa ttatccaaaa cccctcaaca
    22861 aaaagattta atgtcgcttc gcctgtgctg aagtacatta ccgcaggtgt agagatggag
    22921 aatgccgcta caaataagat aacaagcctc aaaagttttt tagattttgt tttcaatttg
    22981 cactccatgt gattggcagc atttcagcta ccaatcctct ttcttatatg atacacatcc
    23041 cattatttat aagttacttg gatgctgtca cttgtaatcc aacgttcgct gcctctgttc
    23101 ggctgaattt taattaagcc attctcacct ttttctaaga ccaaggcagg cccataggaa
    23161 gtggagattt ttttaccggg ttgtacatcc gtaactttgc tgcggtaggc ttgttctcct
    23221 gtatatccat cagagaatgc aaaattcgta tagttttctc tgtaccacca tcccatccac
    23281 tggacttgtc tgccgttgat acagaaaata tcggaagtca gggtttcaac cgtcccctga
    23341 tgatgttcgc aatatgcttt gaaagttcct actgcatcct caaccgtttt ttcatgttcg
    23401 gattcggatc ttgcatagaa ttttcgttca atagttcccg tactttcaaa aatcttgcct
    23461 tggatgatac ggccaagtcc cgccaattta ctgtctttca tccaatcttg tacaaacttg
    23521 ggatagggtt tgtctaaaga agcttccaat accccgtcaa ttttggccgg ctttgaagca
    23581 agattcaaga aattaccttc agcttgggca tttgaaatgg ccatagctgc taataatccg
    23641 aacataattt ttttcattgc aatattttcc tcatcttatt gaaacttagg gtttgttcta
    23701 aacagtggtt tatgatttaa accaactggc catgcgctgc agaatcccat gctttttcgg
    23761 aatttccctt ttcccggagt gagcttggaa attctgatgt tctaataagg ctgcgtattg
    23821 ggcggccaaa gccgaatatt cagcttccaa taaaacatgt ttattcctca gcgcctcaaa
    23881 tttttcctgt atttgctctg cttgttgttc atcggccttt atttgattat gcaacatcaa
    23941 ggccgtctga aaatccgcac tcaaaggatt acggagtagg gtgcagagaa tttgtttccg
    24001 aaaggtgttc cgaagtttct ctacatggcg atttatgcgg tttttgattt cttcattatc
    24061 ataattgagc agctcgtctt catcaatata caaaaatgaa cccttatctg cccgtatctg
    24121 aaccaccggg atacctattt tcgataaggc taaacattta ggcgcattgg aagccaaccg
    24181 tgatgtatgc caaatctcaa ccgccaattt tctctgccac cttatctcgt gcctgagcag
    24241 ggaagtgtta cccggctgat caaattcaag caaaacatca atataaaaag ttttgccatc
    24301 tatatctatc cgatattcgg ataatgcacg gttcagccta atagtgacag gcggttgttg
    24361 ctcggcgtgt tctgtgcaat ttagaacagt cgtcaggccg ccgggtaagt tggatagttc
    24421 gttcagcact tgtttgcaaa gcaagtgggt tagcgattca ggggcggaag caccaccgga
    24481 gtcggcttca ctgtaatagc gaaaaaaatt ggtcgccttc ctcctggtca aaaccatttt
    24541 ctttcttgta ccgggacgat gacggctgta gtagatcatt ccttcccggt gttttagctt
    24601 tgcttccagc gcagttaccc attcatcacc atccggactc gtataagcaa taattttcaa
    24661 acgattaaaa tcctttggga agaaaagcat catcagaata agacaacaat aaaatcctat
    24721 atatcctgtt tgccgccaaa gggcaatttt ttctcccccc ccctttttag atttgtccgg
    24781 ctcatatacg gacatgggca acattataga taacagcaat tattattaca atgttgtaac
    24841 attcagtcta atactgtatt ttgaccatga gaatcttcta agggctgttt tcttttgctc
    24901 tattcctttg atactacttc aagcagtgca gtccaatctg aaggaggatt tccgttcccc
    24961 agcatttttt tgaacacggt atatgcatca tttttgccgc cataagttct tttcgtaccc
    25021 gagtcgttta cccatgcgag aatgatgatt ttactgtttg agtcgtaacg gaaaaacaat
    25081 cggtatctac ccataaactt ggcacgtcgc caaaatttgt attccgggcc gagtgtcttg
    25141 ccaagccgat attccgggct atccggatta agggcaatat tttctatttc gcctaagatt
    25201 cgttccagca gtttggcctc ttcggatcta ctggattttt cggatcgttt ctggctttct
    25261 atcgtagtgg ccagtctgtc taattgccgc gtaaagcatt cgtgttcgta aatggtatat
    25321 ccgtttatct gcatagggga atcaggcata ttagtctttc atatctgttt cccaagtcag
    25381 ttggcgaagg cggttaaagc gtgattgaga tgtgggtttg atggaattcg gattttttgt
    25441 catagaattt tccaaaaaat gcaggaattt tccaacaact ggatcatgct caaattcttc
    25501 ttcctcagta ttgttttttt ctagcaatac ctgtccgccg cctaagacgg aaaaacggat
    25561 tttgtcctgt ttttccagcc ccaacgcctt gcgtactgac tcgggaatgg ttgtttggtt
    25621 tttgctggtc atagttgaca ctgcatcaaa aataatcttc atggtctctt tgccctgcct
    25681 tcattcaaat ttactgttgc tacttgcgac ttcaatgtcc tagttacgca catcttctac
    25741 ttaacggctt gggaaattat tgatattgtt aaaaactgct taaattaaag aattctgttt
    25801 attgtaagga aatttcctta cagggtcaat acccaacccc ttcgacctgc aagggcgttt
    25861 atgaataccc atttactgac tggcagcgat gacacgctta agtgagggag caggcgtagg
    25921 ccggaggtaa ttcctgttcc cgagctgtcg gcggcgagac cgggggattg ggggcagaga
    25981 ccccaaaaat atcagagtat gggcacaaaa aaacggccgc attagcggcc gttggttttg
    26041 aaaaagatta tttgaggcac aaatgctact tcaaacaggc atcgagcttg gattcaggat
    26101 cacactctcc gaaaaactca tccaggaacg ctgataagtc cagttcatac aacgtccccg
    26161 tccagtcgtt ataaaactcc aaataataag agaacgattt gatagggaaa ggctttacat
    26221 ccaatctttc gtatctcaac tttccttcga aaccgaacga atacgcactg cattttgtgc
    26281 tgatgccgtg caaaatgccc tggttttcgt tcaaaagtct aacagcagtg taaagggatt
    26341 caacatcgat gctgacttct aagtcgggta cggtaaagtc atactcgtcc gtctgccaaa
    26401 tggtatttaa ttcgtcacta ccttcgggca ggttaaggta aaggaaacgt ttgccgtttt
    26461 cctgttttag gatgaatcgg ccttcgcatg aagcctgagt gatctctttg tcaatctgat
    26521 tttcaaccat ttctaatctc cacaaaaaag cgggggacaa ccctgcgggt tgtccccgca
    26581 gggtgagttg gcaatacgtc taacgaaatc taagttcttt aatcttcgtc atcgtattcg
    26641 catccaaagt gctcggtagc gaagtcacgg actccatcat ctgtgcacgg ataatcgaac
    26701 aatgctcctt catgaccttc ataggttcct gcaaaccaca ttccttcttc caggtatttg
    26761 gcttcaaatt ctacgtcagg aaaacgtttg gcgagttctc tataaatccc ttccggagga
    26821 ccccaggcag tgtcacagct gatccggatg ccgctttccc caactgtcac aaaagtgcag
    26881 tcggggttta cgtcccattt gcagccccag ttagctaacc gccagtcata ccaattcgga
    26941 tgaccgtatg gcggatgttt tccgcaatac gcttggcaaa tggtccatcg taacggaaac
    27001 gagatgcgac ttcagagtta gcttcaaaat ggagaaatac ttcaccaaca gtcagattgt
    27061 cccaatcaaa tttttccgaa aaatatagct ttaattcaga gaaaagttca tccggcaatc
    27121 tatattgaac ggaccaaaag gatttatcgc gctgttccag catttcccgt tccaaattga
    27181 tcaacttctt ttcaggttgt tgagcaattt gaaaccaggt ctgctgcatg taactatcag
    27241 gcacttcagg aagaatcagc tcctccggca tgggtacaat atgctgaaag ctgaattgat
    27301 accccttgtc ggttttgatc agaatttgct ccgccacttc ggcgatgcgt tgaggattgc
    27361 cttgaaaatg aaggctgttg ctgcaccagt tgggcatagt agtctcctga gcaggaatcc
    27421 ccatccctgc ggataaggaa acccgcaggt tgatgggctt gcgcccgggt tgataaaaaa
    27481 gccgccaccg gaaaccggcg cacatttcag acgacaggtg ctttagataa aaggcaaaga
    27541 aaccgaaacg gtaactttgc ctttgggaag cggaacgacg gttccgccgg cttgatgaaa
    27601 aatgtgctta agcacggtta ttttttgggg gttttaagat agattctcgc aacaataaca
    27661 agaaccaggc cgactgcggc catgacccaa gttccggtaa cgaagtccat tatttgtcct
    27721 ccagtttaga gatgtgaatg cggataagga atgaaacata cagcatttca aacgacagga
    27781 gcaccaatcc tgccatggca agattcttca tctcctcact atatccgtta ctccagacca
    27841 ccacaccgat accgataatc gccgctttgg caatatcgtc cagtatggcg gcccatgaag
    27901 ccagtaccgc agtgggtact tttgttttcg atttcatatt gtcaggcata tacacctcca
    27961 taaaaaaagg agatgtacgc ctgacgggga atacatcccc ttcagggttg ataaaagagc
    28021 cgtgcatccg aaacggatgt gcatttggtt aacggcggta tgcgggcggt ttgacggact
    28081 gcctaccgtg taacatagtc taatcttcta ccaaataccc gtcgctttca agtctatcgg
    28141 acaagccttc gtaccaaata tcgaattcgg cgatactgaa tttacggatg tcggccatct
    28201 cgcaaagaca gtggtaaagc acgctgttac ggtattcgta gcgtccgccg aaacttccgt
    28261 actggttgat gtatttcgct gtaaaacata catccaagtt ggcgaagatt tccgcgagtt
    28321 tgcgataaat accgaaaggc ggtgccaatc cggttaaaaa gcagatttca ataaaaccct
    28381 ctctttgtgt aacaaaggtt tcggtgggat atggggaaaa actgctgccc catgcctcga
    28441 ttctctgttt ggcaaggtca gacgtgatat tttcgggtaa aggcaaaatc cgattgaaat
    28501 caaaagccca atgctcagga tgaacggggg agcgaaataa aatcagtttc gccgcttcgg
    28561 caaccgattc tttggttcct tctattagaa ggatgttgtc aactttgata gacataacta
    28621 tctccagtag agtctctgcc cctgcggcca aaagaccgca ggtaatggaa tgggcattta
    28681 cccagttgga aaagaagcac ggcggttatg cctacacatt gtacagctcg ggcaggattt
    28741 cgcggttcag ccagccggtg acgacttcgc gcagcacatg gcggcagcca tcagtcagaa
    28801 catcagatag actgaaaccg aactcgtaca gccccagccc ttcattcgac tgccactcga
    28861 tgatggcatt gatgtggcgg cggaaaatcc aggcaatgtc ttcatctgat aaatactgca
    28921 atgcccccct gaaaaataac cattttgtca ggacggttga aatactgccg ataggcaggc
    28981 atcagatagc gcatgtcggc aacggcggat acgcctttac cggcgtatcc gtcctcaagg
    29041 taagtgacgg ctgcaacggc atcaccattg ctactgctga tgcaaaccat gtcggcatta
    29101 gcctgtttgg caaactcttc acaggtttgg gcaaagacgg ccaaatcagc ccctaatcca
    29161 aatcttttca gttcacgtct aatagcggcc atgacaccat ccgtgtgagc ggtcaaaact
    29221 gcaccgttca atttagctgc cgcagctgcc gattcttccc gatcgggatt aaagttttgc
    29281 attgtgttgc tccttaaaaa gaaaacggag aacacagtcc gtagggattg tgttccccac
    29341 ggggttgata aaagaaaatg ccgtacatcc ctaaggatgc gtgtcatgtt taacggcagg
    29401 acacaagaca gataaggtct gtcagcctgt aaagacaggt attttcagac ggccttacta
    29461 aagccgtctg aaaatacctg cccgaaggca ggtgagatat ataaaaagat tacaaggtca
    29521 gtaatcttct gggaacagga gtgtggtaag gcttctgtcc cattctgtaa caacccaaat
    29581 tttttggtcc ccaatcttgt aagaagacat tattctgcct tcttcacctg gcttgagtgc
    29641 cagctgattc ggcttggcat cctcaggagg aatatcaccc catatgccat ggcggtgggc
    29701 ctcaagcaat atttgggcat aaagactgtt ctcatacagt tgtgcaggca ggtgctcttt
    29761 tgcccggtaa tctactgttg ccgtcataga cactcggtcc aggtcaaaca gagagggggt
    29821 atcacatgcg gccggagctg ccgaatcaag ttgttccatt aggcgtatcc ttaaaaaaaa
    29881 accggataca cccctgcggg aagtacccgg cagggtttaa aaattgaata ggccaatcta
    29941 tttgattgtc aggactcgtc caaaatccga cgatccttcg gtcaaatcaa ttgcgcgtat
    30001 gcttggagaa aaccggtcag ttgaagtaat cgtgaccaca agcctgtcct tcttgccgta
    30061 ctcttccgat ttggtttcca ctttggtttt atgggttgaa cctttaacca gcagctttcg
    30121 gccattggtt gaggtgacaa cgccattaac ttgccctgcg gctaatgcta aagcagcatg
    30181 ccactgaccc agagcacata aaggccggcg tttttcggct ataacggcac tggtgccgaa
    30241 atattgcgaa aatgatggcc ataacgtgcg ggattgctgc tgtcccagtt cacgttccaa
    30301 tatatcctgc tccaatttaa agcttctggg gcgaaagacc ttattgggtg caataggcac
    30361 acagtatgca ggcataccgg ccggcaaagt ctcaggggca ttttcaaaat caagcaggag
    30421 cctttgttgg gcatccgcct gttttttgct gatagaggct ttgttcttcg gtcggatgcc
    30481 gaatataacc aactgtttgt aggtatccgc cgcagccctt atcatacgga catccgtaca
    30541 gtgggtagcg atttcgccgg ttagatgctc tgtcagggcg gcttccggaa caatcattac
    30601 caacactccg ccgggctgca aagtaccgaa ggtacgtgcg aagaaaagtt cttcaagccg
    30661 tttggttctt tgattagaca gattgtcttt ggcggcaaag ccgtaaggag gattcagaaa
    30721 caggaggccg actgccgaac cctgcatggt gcagttttct acatcggatt tgatgacact
    30781 ggtcagtcgg ctcattgcct cttgagcacg gtctttctcc agttcaatcc cgtaagtttg
    30841 acaacttatt ttttccgatt gcaaagtaga agctaaagaa gccagggctt ctcctgttcc
    30901 gcagcaagga tcaaatatgc gtaacaaacc tccggcagat gccggaatat caaggcaagc
    30961 ggctatacgc gacaatgtgg cctggtcggt ggggaagtat ccgttgtcac ggtggttatg
    31021 cgcgacacgg gggtgcatca gatggttttg agtttccata ttggtctcca ttcaaataaa
    31081 aaacggagac atgccgtgcg ggacagtccc cgcagggtta aatcaaagtt taatcgaagg
    31141 taccgccacg ttctatatga cgtaccattg ttttaaaatt cagcaatcca cgcggatcct
    31201 gtcccggcga gccgggtatc ggaatttttc gatcggtcgg taggtgcttc aatgcgctgt
    31261 gcttacgacc gtttttgggg aggaacatcc attctccccc ccgataccag ttggtgtata
    31321 aaaaattgat aatctttgtt tttgctatat ctcatttctt ctccgataca aaaataggtt
    31381 ttcctcctgc aaatggttaa aaatacacgc atacgattaa aaaaaacgta accgcatgac
    31441 ttccaagaaa aatcccactg ccgtacagac gtacggaaca gcgggattgc ggcttcaagc
    31501 cggatttatc taaaaaggaa cgtatcagcg ttctatttaa aatgctttgg tattaaatag
    31561 caacaaagca tttaaaagat gggaatacca attaacttga ccggtcatgt gtattagtgt
    31621 cttgctgaga ctcgccaaac tttaccagtt taaatatgcc aaacaacatg acggcaatca
    31681 aaaatagtaa gctgtaggtc atgatcatgg tttttccctt tccttgggtt cctgtttgac
    31741 aatctttgat tcatatttat ccaagtaaaa gataaatact tgagctacat agaatgaacc
    31801 gcgttaacgg tttttgtgtt cttccgacgt acgcgaagtt tcaagctgct tggtaatcca
    31861 atagccgaaa gcaagcacca ggaagagaat aaatagcaga ccgcctatca gcatgacgta
    31921 tcctttctgt ttaatgaatc acggtatttc cgtagtttgt cagcatatac ttgcacaaag
    31981 taggcaacca aagccaatac ggctacgccg gaagcacgtt cggtcaaaga gtatgaattg
    32041 agccaaaaca acggcagcat tgccaacaag gctgttttac ctatatcttc cagaaatttg
    32101 atccaagatt caatgctttc cttgtgaaaa ggaaggtcgg gtttgatgaa tataaaaaat
    32161 ttcacatcgg tatctccatg aaaaagggag atataccgat gccctgtggg gatacggtat
    32221 atccccacag ggttaaagaa agttgaaaaa gagccgtccg tcccgaagga cgtgcgcaat
    32281 gggtaacggc agtgcgcggg cagataaggt ctgccagcct gtgaaattcc accttacggg
    32341 ctttttcaga agacctcaac acgaggtcgt ctgaaaaaac ccactaaacg cgggttagat
    32401 gcaaaaattg catcaatctt gtcggtcaac gaagcgtttg gccaactgaa cttcaactga
    32461 accttcagcc tgattcagaa tgtccagacc cgaatcgggc atatcaccgg aggttgactg
    32521 agtaacggca actttttgcc ccatcagttc aaggcaaatt tgctgcgctg tatcgctgta
    32581 accggcgaaa tacacttcca cgtcttcctt ctgaccaata cgccaagaac ggcgggcagc
    32641 ctgcatcagg gtataaacgt tatatcctgt ttgcaaaaag tagatggttg gaaatgccaa
    32701 caagtccaaa ccggttttga ccagctccgg attacagata acaacctgta cgcctgaagc
    32761 caattggtct tcaacccagt cttcgcgttg atcggctttg accgtcgctt tcattaccgc
    32821 cgccttaata ccgtgccgtt tgaaaatctc tttcagacgg cctgtcgtat cacgggtatc
    32881 ggtataaaca gtatagacca gacatttacg tcctttagcc agactgtctt taacgacttc
    32941 cagcatgtca gcttctttcg gtgagatttt ttcttcatcg aactggggtt cggtatggaa
    33001 aagaatcttg ttttgtctgc gccagtaaac atcttgaggt ttgtaacaac aatccggcca
    33061 tgacagcaag gcgctcataa caacgccggt aagggtattg tcacgattcc ttaatgccac
    33121 cttcaaatgc tgcctcaaaa cactctccat atttttatag accgcctgtt gatcttcagt
    33181 catttcaacc ggccggaaaa cttctttata gccaggtaaa acgccttctc ccaaatcacg
    33241 cagcttaaga aatacggtaa taggcaaaac ataacgcatg atgcccaaag gggaaaatcc
    33301 cggcgccttg gcggtgcgaa cgctgttacg ctctgctttt gagctactga atgcgccatt
    33361 actgtattca gttccaagat gtcggacaat atccttcaaa acgccatgct tacgcataaa
    33421 cgccatgctt gccgaaccca aggtattccc tttgttgtaa ctgaaaccgt cttcaatcat
    33481 catttgcggc caaagccgcc acaatagaaa gaacaggtct tccgcataac cacccatcag
    33541 cgtaccggtt aagcacagga tcttattaca gcaacgtgcc aaaacgccca tggcttgacc
    33601 ttgagccgta ccgtagttct tgtactcgtg accttcatct actaccaaca gcccgaaaaa
    33661 gttcttcggt aaatagcgtt tgacgaactc ggtcggctga tacccgcctc gtcccaggct
    33721 gaattctgct ttgcctaagt ctcggtcgat acgcgccgcc tgacggtcgt cgaataaaaa
    33781 ttctccgttt tcatccatca aattggaaaa cgcctgaatg ttgttttcca agatttcagc
    33841 cagatactgc tcaccataca tcccgatgat ttttgctgcg gttttaggac cgatgccggg
    33901 tagggtggtc atggttttaa gtacgcgatc gtacactgtg cttacaaccc cgtcttcact
    33961 ctgttccttg cggcacaagg tccagagcgg ttccttacac ggtttgccat gatgattgcc
    34021 tttgcaaaaa cggcggtttt tagccagggc gtttttcaca ccaccttcag tgttataagg
    34081 cttgttttcc tcatccaaga tttcacagcc acatttagga cagtaatact cggaaacggc
    34141 aaaaccctca gaagcacctt cagaaggtat ccggcggcgg cgaacggcat acgaaggcct
    34201 ccaatggtag cccatgcgca tccgaacacg ccccatcaca aaaaactccg gaacttcggg
    34261 tttgcggtca agggcacgga tttgcaacag ttttgccaat gtatcggctc cgttaagaat
    34321 ccatacgcgg gcattgggta ccgttttgag gatctctcgt ctccacttgt acaccaaatg
    34381 aggaggactc aataccaagg tgcggtgcaa acctgcttga tgcgcggcag cggctgctgc
    34441 aaccgacatc atggtcttac ctgtgcccat ttcggcatta ataatggctg cgggtttgtc
    34501 ctctgccacc aattgctgta atacggcgcg cacaacttcg cgctgcgccg caaacaaagg
    34561 acgttccagg ttatccagca cttcttcaag gagaggataa gtttgtccct gatagaccgg
    34621 ttggttttgc tcagtaactg cgttaaagag actatcccca aactcggcaa gaaactgtgt
    34681 caggggtatg tcggaatccg gcagttcagt ttccggagat tcctccggca atacggcagc
    34741 ttccgtactt ttttgggcag tctcagccac ttggcggatg ataaagttga aatcagtagt
    34801 tatttgattc tgacgctcct gcatctcggt cacagtttga tccagccctg caaaatccgg
    34861 gaggagttcg tctatttttg atacggggtt atacatcgta tgctcctaaa aaaaagacgg
    34921 agcaaacccc ggcgacgggg gattgccccg tcagggttag aaaccgctac cgtatggtgc
    34981 ggaaaaaggg gttagcgaag attcgccaaa ggaaaaaata gatgcttggt ggcatcttcg
    35041 aaagaagtaa agccgaaatg cctgtaaaag ccgatggcag cttcgtcttt tgcctccaca
    35101 atcaacgcat acgcggcgat ttctgcttta ctgatacgtt tgagtgcatc agccaaaagt
    35161 aaagaaccca gtccaaaccc gccatatttt ccatctaccg ccaaacggcc catcagcact
    35221 gcgggaaccg tagggtatct gggcagcttc ttacgaaaac cggcgggtat attacttagg
    35281 gcaactcctg ttgcagccaa tgtatagtag ccaagaattt gaccgtcgga tgtaaccgcg
    35341 acaaaacaag tagcgacttt ccgttttacg tcttgcgaaa cctgtttttg cagatagcgg
    35401 tttaaaggtt cggaagggcg ctcaaatgca ttgcggtcat cggcagcatc aaggaggcgg
    35461 attcggaagt ccggtaacgg cattttatct cccgaacaat tcgtcatgtt tttccaatgc
    35521 tctgctcaat gccccattcg gtttggacgg attgagcagg gcttgggcaa actgaagttg
    35581 gtcacgttcg gacagcatca gaatctccgc atcggcaact gctttttggg cagcctcgcg
    35641 ggcagcactg atgacaaatt cggacatact gcgtccttga agttccgcag cgtgtttaag
    35701 taaagcacgg gtatctgaag tgattcttgc ttcaaaacgg caatcggcat atgtggcagt
    35761 catgaacata ctcctaaatt atttctagat tgtacggctg attaccgtac aaaacaagat
    35821 atgaaagggt attttgtaga aaacatcaca tgctccaaag taacggagat atacgatctc
    35881 cataggtatg tatatcccca tagggttgag tgagaagtga taaaaatccc accggatgcc
    35941 ggaacatggt gggattcaag gttacagata aaaagctccg tttaacggaa cgggtttaat
    36001 gttacaggaa cttcgcagca gcaacggcaa cggtagtcgt gataccgata aaccaataaa
    36061 ttgcattgat tttagtatcg attgcgctaa accgtgcatc tacacttttt tcaaatgagc
    36121 tgatacgggc atctacactt ttttcaaatg agctgatgcg ggcatctaca cttttttcaa
    36181 aagcgtccag tcgggcatcg aagttctggg tcatgaaatc cagtttgttc aaaacctttt
    36241 ccatgtcgtc ctccgttttc aaatcgatta aggctactat agcagctgca tcctgacggt
    36301 tcaagccgga cagtacctca tgcttttcag atagagtagg cataaaatcc tcctaagttt
    36361 gggaatatgc ctccccatgg gacgtatatc cccacagggt tggtaaaatt gaaaaatgcc
    36421 gcaaccatac gaatacggcc ggataacctc agcagcaata tccatcaacg gaatatacag
    36481 tccgtcatct gtgtaaaact cttatgtaac agactttttc agagagcctt tgcaaggccg
    36541 tctgaaaaag cccgctaggg gcttcagatt aagtatgtaa gcggccgctt tgtagcatag
    36601 cggtcaaacg cacatcaaaa tcttccggga tatcaactcg taccgcttga actccaacta
    36661 ctgcgtactc ttcatctata cgaggagtaa accgcatgat ggatttatca gcctcaagta
    36721 ccgataaaac agcattctgc caatgttcca ataacggtat atcggagagt tgccgcacca
    36781 gttgccaagc tgccttgtcc aagtctgcgg taacatcatc caacataacc caacctgttt
    36841 tactatcgaa gttaggttga atcagaatat ccgaatacaa aaacatgtgc gtgaccgaac
    36901 cataattaag agtctgatat ttcgtcatgt atttgctcag attcaagcaa tgctcggcag
    36961 tcgtcaggcg cggacagctt aaaacctcct ccggccgtct aaaaccaagc cgttcattac
    37021 ttacttgcag ttgagcttgg aaggacaaca tgtctgcatc gcggccgtgc agagaagcaa
    37081 acatcaactg tcggttccaa atgaagaagg catcggccat caaatcattg cgggaagtca
    37141 ggtaaagcat tccaacctcc tattgcagac accaagtctg cgactggggc cgataggaat
    37201 accctgcctc cttcatcatt gaacgggcta tgttcagctc gtcacggtct gaaaggatat
    37261 actcacccaa actgatatgc tcccggtttt gggcgatcat ttcgccgacc tgatccgcag
    37321 tttccgcgta ttcaggcaac cggatgccgt tttcaggaac cggatcgaac ggatcggatg
    37381 gatcatcttg cggagaatcc gacccggaag aaaattcctg cgcaaattcc gccagatgtt
    37441 gccagtcaag agctgcatct aagtaagagg tggcgccacc accccactca taagccttaa
    37501 ctttgagctg ttcaataatg aagtttcctt ggtattcacc aggctcaaac tgtttcaata
    37561 ttgggttttt tacatcaaat gttccaattg atgtagctaa tttagctgtt ttgaaagggc
    37621 ctcggcgacc ctgtatgact tgcaccgtca aggtgccgga aagattgatg gacatggtat
    37681 cactccaaaa gaaaagggat accggccgcg ggcagatatc ccgtggttgg gaaaaaacaa
    37741 attagcgctt aatagcactg taaatcttat ccaaatatac tttttgggac atacgcagtc
    37801 ccaatcttgt aaaaatacgt ttgtcgttat ccaatgcccg aatagccata ttcaacgcta
    37861 cggaagcgtt tttatggcta tgaacatcgt tgacgctcct acaataccga ttgaccgccc
    37921 tttgggttcg tattttcgaa atacggccat ccgcatcaac aagtaacaaa tgatgttctt
    37981 gtatgcatga gacgacaact ttccgatgag tatgtcggtg ggtgaggatg tcaccgatac
    38041 gtattctttc cggtagtaaa tttgggggaa ggatggcacc tgaacggtga aaagatagat
    38101 ttagattcat ggtaatactc caaaataaag ggtacaccat gcccattcgg ggaagtgtac
    38161 cccgatgggt taaaagaaat ctgcttcaga aaaaagcagc tgtaccgtac ctgaaacagg
    38221 cttgcaatca acggctatgc aatgacagaa aaaacagtct gtcagctgta taaaactctt
    38281 gaaatacaga ttctctgagc cgttctgcaa atatcaaaac gtcttagaga acctgcaaag
    38341 caggttcaaa tggggattac cctggctaat gattacctag tccgcaccga cgaagtaatc
    38401 agataatggt ctggatagta ataaaaccgt aacaattacg aataattgcg cgcactggtt
    38461 atacggctat acgcagacag aagaaacggt ctgtcaaccg gggaagctta cctgaatgac
    38521 ctatggtagg gaggggcatt caggtaagct gcccaaaggg cagccttcaa acagttgctt
    38581 caaaagcaac aaaaataaaa aattcggatt gttaaagaac tgtatttttt tactgatgct
    38641 cacaatagat atgagcatcg ataaaaaaac aatattgctg aaagccaagt ctttgagggg
    38701 acttaccctt tgcttcacta taattcagga gttgcttgga aaattaaacc gccgtttcga
    38761 cagaggctta attttccaag caatgagctg gaaaactaag aacggggaag cccgaaagct
    38821 tcctaccatt cttaaataat caaaatattc aatagctttc taagaagcat caggattatt
    38881 ttgggtttca tcttgagagg atgattgata ccacatatcg ccatccactt taataaattt
    38941 gaatttcaac aaccgaccgt cgatacttaa agtttctaaa acttcgcccg tctcacgatt
    39001 gttagtttca aattttaaag gttgaatatc accggcacgg aaaccgacca cgacagtcgg
    39061 gcgttcacgg taatttttag acagcaattg cggaaattct tccaaaaggc gagcaaagag
    39121 ctctttcgct tgaccaccaa cgatacgaag atcgaaacgg gttttctccc cattatcacc
    39181 tcgagaggct tggatcgtac aagcataata gttggaagat tttctctttc cttgaacttc
    39241 acgtggccgg tttaaaaaac cgataccatc tgcagtcagg ttaaagtagt tttgattaga
    39301 ttgagtcatg atatatctcc aaaaaaacag gggatatatc aatcccaaag gggacagtat
    39361 atccccaaag ggttaagttg cggctttcac ggtagccgaa ctgacttaga agccttaaag
    39421 gctggctgaa aaagaagtac cgtaaattcg acgaatcgaa tctcattcat gaacggctaa
    39481 atgacgagac ttttaagaaa agtcacaaac tacaaaaaca attacaatat attcccgata
    39541 atttcgtatg tcaatatttt ttcgtaaaat ctttaaccgc gccaatgccg aaccctgccg
    39601 gtatccaaat ggacaaaact attatagata ccaataccgc ccacattgta gtatttagcc
    39661 atttgctcaa gctcgccaat gcccaccccc ctcatagtaa agtcaactgc tttaccacgc
    39721 atatgaagtg agtttctagc agcaccctca atagtcgcat ttcgtcttgg cgttctatat
    39781 gcactattga tcgtaatcac agggtttgac cgtcctgatt gccgtgccca ctcctgcatt
    39841 gcatacatca ggtttaaaag ccctacgtca atttgaacca tggcatttcc atctttcgca
    39901 tcacgcatca gccaacaggc attgcggtaa gcatcaagat catatccatt ttgttgctga
    39961 aaaaaacgga tttcatgttt ctcgccagta tctgcccgtt tgcaattgat tgtacggtct
    40021 cgtgaccaaa aatcagccat actggttgcc gctactacat ctttaggcaa taacaaaccg
    40081 gcagcaccgg tcgtatatag ggcgccaacg ccgacaaact ttttcaaaaa atttcttcta
    40141 tccatctgaa attccaaacc aatgactcca atcaaatttt aagcaacact cagtattgat
    40201 atgcaaactt cccagtatgc tggtagaggg caacatcttt gagcttcctc aatctctgac
    40261 agccacaata tccatcgagc acgttttgtt actctgtata ttcttagaaa gttctttatt
    40321 aaaaattgaa aaaattgaat cttggttaaa aaccaattta acaaaagtgc caaagaatga
    40381 tatacaataa aacagacgaa aaagtccgga tattgctttt gcaaaatgtg ccggatcctg
    40441 taacatcatg aatactcgat gaaacgcttc aggattgtcg gtgttcaacc acagtaaaaa
    40501 caagacccca gcggcagggc agtaagcgca attattgcgg gtttcaataa aaatcctagt
    40561 atagatttaa agatactcca ccagaggcta ctagaataaa acactctata ttgcaattct
    40621 tgcagttcgc caggtgaaat actccctact tgaatgccat tgatgtaaat gaataaatct
    40681 ttattagcgt tgtattgctc cttgtaatta tggttttcat ctagcacata ctctatacaa
    40741 gtatttccga atattgaatc ggataatttt tttaccagct tcttcatttc aaagtccttt
    40801 tttatttgac ccattcgaag tgtttacatt cgggaaacct actacacccc caataccgct
    40861 tatcagggtt ttccttgaat cctctctgtc tcagacggcc ttccttacag gtcggacaga
    40921 catcgtcttc atttcgagcg accgcctgtt tttttgtacc agctgaatcc gtctgttttg
    40981 gcggacgagc ttttcctaca gcatcctttt tcgcctgccc aaccagctct gtgatcactt
    41041 tctgctgata agccataaat tcagctaaag ttaatttgcc ggcttctatc ccagccaaag
    41101 ccagctccca tttggctgtc gttatcggat cggcaatcat agtgggaata cgggcaatca
    41161 gattacggcc tttttctgtc gcatagattt tgctccgttt cttttccata taacccattt
    41221 caaacaaacg atcgataatg gtagcgcgag tggcttccgt tcccaagcct tccgtacttt
    41281 tgagaatttt tctgatctgt tcgtcttcta cattcttcaa aaagtccttc aaagtctgca
    41341 tctcatcgag cagagaagcc tctgtaaagc gcggtggcgg cgtggtcttt ttagtttcgg
    41401 gattgaagga aagcgtgtcg acctcatcgt tgactttcag atgtggtaga gcaggacctt
    41461 catcgatttg atcgccttca aacacgtcct tgcccaaaag aaccttccaa ccggaaataa
    41521 ccggtgattg gctggaggct ttaaacaaat gtgaacatgc ctcaacctca actacagtag
    41581 agtcatattc aaaatctgga taaaactgtg cgatataacg gtctcgtatc attcggtaca
    41641 ccgttaattc attcttatcc aggactgaca aatcaaaatt actggccttg gtcggaataa
    41701 tcgcatgatg ggaatgtttg tttacctggt tatcgttcca tacccttgat tgacgttcgg
    41761 gatcagcttt ttcaatcact gaaagcatgg atttatcgat agtctgtatc gcggcaaata
    41821 catctttcac ttcactcatc tgcgattttg gcaagtatct gcaaggggtg cggggatatg
    41881 acgtgatctt gtatttttcg tagagggttt gtgcaatttt gagcactttg gaagccgata
    41941 ggccaagttt tttaccggct tctttttgca aagccgaaag gctgtacggt aacgggggcg
    42001 gggtgctttt acgctcttta tttgaggtaa taaccttgcc tgtagatttc tcaagctgtt
    42061 tgacaagtgc ctttacagcc tcaaggttgg tacagtatcc gtcagtatta cgccagtttt
    42121 caggaacctg ccacagtgta gtaaacactg acttgtctgc tgtctcaaaa tctactgaaa
    42181 gggtgtagta gttgtaaggc acgaaagtat cgatctcttt atccctttgg actacaagct
    42241 ttaaagtcgg tgtttgaatc cgtccgatcg acaaaatatg ttctcggcca tatccggcag
    42301 aataagcagc tgtgtaggca cgactcaggt tcatacctac cagccagtcg gcgcggccgc
    42361 gcgccaaacc ggcctgatat aggttttctt tatccttacc cggcattatt gaagataagg
    42421 ctttttttac cgattgggta tctaggccac tcgtccaaaa tcgggaaatc tttttacggt
    42481 agccacagta gtccaatatt tcacgggcaa taacttcccc ttcacggtct gcatcagtag
    42541 caatcactac ttcatctgct ttggccaaca agcttttaat gactttaaac tgcttcgata
    42601 ccttcggcgg tacctgcatc tgccaaactt cagggagaat gggcaatgcg gagatatttc
    42661 caaagtcagc atattgttcg ccataagcat ctggcgcggc ctgtgtcagg atatgaccaa
    42721 atgcccaggt aacaatagtg tctccattct gaaaataacc atcatgcctc ccgccaagaa
    42781 caccaagcac tttaccgatg tccttagctt gagatggttt ttcacataaa aacaaacgca
    42841 ttttaaaatc ccaagctttg cttcagctgc aaaacaagcc aagatgcgat agtcaaaaaa
    42901 gcccaaattg ccaaagcaat taagccacca taaataaggg taacaatgaa catatcctta
    42961 aagtcagccc ataatgcagc aagccaacag ttcatttcac gaaaaaagga agtattcttc
    43021 gtggccatat atttacaatg ggatgtcatc atcagcgtct tcaacgtgat tattgtcctg
    43081 agactgctca cgtttaggac gcaacttgat agcttctaca cgctcagttg gaacaatgaa
    43141 tacatcttga acacgcaatt tacgaacgcg aacttcaata ccgtcacgat tggtataaga
    43201 ctcgtaacgt tcttcaccgg taacgaaaac cggcataccg acctgtaata ttttatggag
    43261 atgggcagca tcacgattcc agtattcgca agaaatccac tccgttgaaa ccacttctga
    43321 tttaccttcg gcattgcgtt tgaaattagg tgaggcaact gaaaaattca gtacgattga
    43381 ggcttcatcg tttttagggc ttgctacagg acgcagatta aattcagcac cgagattgcc
    43441 acgaacaaaa agttgaactg acatattttt ctccaaaaaa aggatttaaa aatatgacag
    43501 aaaagacagg gtaaaataac ccaatatgag attaagggtt ctattatcat aactaaataa
    43561 attcctcttg tcaactagca actattacaa attaaacctt atgctatatc tagctaaaat
    43621 gcccacg
//
LOCUS       DAXFWM010000010        39881 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000010 DAXFWM010000000
VERSION     DAXFWM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39881
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.010"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            104..490
                     /locus_tag="ACFI8B_000502"
                     /pseudo
     CDS             104..490
                     /locus_tag="ACFI8B_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(873..1763)
                     /gene="sucD"
                     /locus_tag="ACFI8B_000503"
     CDS             complement(873..1763)
                     /gene="sucD"
                     /locus_tag="ACFI8B_000503"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC3833795.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTNLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1774..2994)
                     /gene="sucC"
                     /locus_tag="ACFI8B_000504"
     CDS             complement(1774..2994)
                     /gene="sucC"
                     /locus_tag="ACFI8B_000504"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC3833796.1"
                     /translation="MGLAAGRFLKPTHLKEKSMNLHEYQAKELLASYGLPVQGGILAH
                     NGEEAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTY
                     QTDANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETP
                     EKIFKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVN
                     PLAVRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEG
                     NIGCMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVL
                     INIFGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADG
                     LNDAAEKIVAAVNA"
     gene            complement(3012..3299)
                     /locus_tag="ACFI8B_000505"
     CDS             complement(3012..3299)
                     /locus_tag="ACFI8B_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833797.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(3419..4852)
                     /gene="lpdA"
                     /locus_tag="ACFI8B_000506"
     CDS             complement(3419..4852)
                     /gene="lpdA"
                     /locus_tag="ACFI8B_000506"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3833798.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(5113..6294)
                     /gene="odhB"
                     /locus_tag="ACFI8B_000507"
     CDS             complement(5113..6294)
                     /gene="odhB"
                     /locus_tag="ACFI8B_000507"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC3833799.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(6394..9222)
                     /locus_tag="ACFI8B_000508"
     CDS             complement(6394..9222)
                     /locus_tag="ACFI8B_000508"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC3833800.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYVGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(9427..10710)
                     /gene="gltA"
                     /locus_tag="ACFI8B_000509"
     CDS             complement(9427..10710)
                     /gene="gltA"
                     /locus_tag="ACFI8B_000509"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC3833801.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10822..11070)
                     /locus_tag="ACFI8B_000510"
     CDS             complement(10822..11070)
                     /locus_tag="ACFI8B_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC3833802.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(11074..11781)
                     /locus_tag="ACFI8B_000511"
     CDS             complement(11074..11781)
                     /locus_tag="ACFI8B_000511"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC3833803.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11901..13664)
                     /gene="sdhA"
                     /locus_tag="ACFI8B_000512"
     CDS             complement(11901..13664)
                     /gene="sdhA"
                     /locus_tag="ACFI8B_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC3833804.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(13667..14008)
                     /gene="sdhD"
                     /locus_tag="ACFI8B_000513"
     CDS             complement(13667..14008)
                     /gene="sdhD"
                     /locus_tag="ACFI8B_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC3833805.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(14002..14379)
                     /gene="sdhC"
                     /locus_tag="ACFI8B_000514"
     CDS             complement(14002..14379)
                     /gene="sdhC"
                     /locus_tag="ACFI8B_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC3833806.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(14666..16069)
                     /locus_tag="ACFI8B_000515"
     CDS             complement(14666..16069)
                     /locus_tag="ACFI8B_000515"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3833807.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(16318..17055)
                     /locus_tag="ACFI8B_000516"
     CDS             complement(16318..17055)
                     /locus_tag="ACFI8B_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC3833808.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(17334..19610)
                     /gene="metE"
                     /locus_tag="ACFI8B_000517"
     CDS             complement(17334..19610)
                     /gene="metE"
                     /locus_tag="ACFI8B_000517"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC3833809.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNAGQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19746..20627)
                     /gene="metF"
                     /locus_tag="ACFI8B_000518"
     CDS             complement(19746..20627)
                     /gene="metF"
                     /locus_tag="ACFI8B_000518"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC3833810.1"
                     /translation="MQNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSI
                     HRLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIA
                     KDYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKS
                     AQADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQ
                     LGKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTL
                     NRSELTYAICHILGARP"
     gene            20818..21093
                     /locus_tag="ACFI8B_000519"
     CDS             20818..21093
                     /locus_tag="ACFI8B_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC3833811.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            21093..21218
                     /gene="ykgO"
                     /locus_tag="ACFI8B_000520"
     CDS             21093..21218
                     /gene="ykgO"
                     /locus_tag="ACFI8B_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC3833812.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(21304..21522)
                     /locus_tag="ACFI8B_000521"
     CDS             complement(21304..21522)
                     /locus_tag="ACFI8B_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833813.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            21665..22804
                     /locus_tag="ACFI8B_000522"
     CDS             21665..22804
                     /locus_tag="ACFI8B_000522"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC3833814.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22801..23382
                     /gene="metW"
                     /locus_tag="ACFI8B_000523"
     CDS             22801..23382
                     /gene="metW"
                     /locus_tag="ACFI8B_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC3833815.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            23499..24302
                     /locus_tag="ACFI8B_000524"
                     /pseudo
     CDS             23499..24302
                     /locus_tag="ACFI8B_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            24294..24401
                     /locus_tag="ACFI8B_000525"
                     /pseudo
     CDS             24294..24401
                     /locus_tag="ACFI8B_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(24477..25037)
                     /gene="efp"
                     /locus_tag="ACFI8B_000526"
     CDS             complement(24477..25037)
                     /gene="efp"
                     /locus_tag="ACFI8B_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC3833816.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(25080..26231)
                     /gene="earP"
                     /locus_tag="ACFI8B_000527"
     CDS             complement(25080..26231)
                     /gene="earP"
                     /locus_tag="ACFI8B_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC3833817.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(26250..26528)
                     /locus_tag="ACFI8B_000528"
     CDS             complement(26250..26528)
                     /locus_tag="ACFI8B_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833818.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26879..27820
                     /gene="miaA"
                     /locus_tag="ACFI8B_000529"
     CDS             26879..27820
                     /gene="miaA"
                     /locus_tag="ACFI8B_000529"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC3833819.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEIYYLTGRPMSAHLNGQPEHTLPFELYTAALIPEDRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27824..28543
                     /gene="tadA"
                     /locus_tag="ACFI8B_000530"
     CDS             27824..28543
                     /gene="tadA"
                     /locus_tag="ACFI8B_000530"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC3833820.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMPQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            28548..29036
                     /locus_tag="ACFI8B_000531"
     CDS             28548..29036
                     /locus_tag="ACFI8B_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC3833821.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            29102..29854
                     /gene="rlmB"
                     /locus_tag="ACFI8B_000532"
     CDS             29102..29854
                     /gene="rlmB"
                     /locus_tag="ACFI8B_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC3833822.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEGILENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCNLPDSTAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29916..31291)
                     /locus_tag="ACFI8B_000533"
                     /pseudo
     CDS             complement(29916..31291)
                     /locus_tag="ACFI8B_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            31671..32546
                     /gene="dapA"
                     /locus_tag="ACFI8B_000534"
     CDS             31671..32546
                     /gene="dapA"
                     /locus_tag="ACFI8B_000534"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC3833823.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            32555..33751
                     /gene="bamC"
                     /locus_tag="ACFI8B_000535"
     CDS             32555..33751
                     /gene="bamC"
                     /locus_tag="ACFI8B_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC3833824.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERRAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33808..34740)
                     /gene="pip"
                     /locus_tag="ACFI8B_000536"
     CDS             complement(33808..34740)
                     /gene="pip"
                     /locus_tag="ACFI8B_000536"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC3833825.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
     gene            complement(34733..34996)
                     /locus_tag="ACFI8B_000537"
     CDS             complement(34733..34996)
                     /locus_tag="ACFI8B_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833826.1"
                     /translation="MNQALPRRLEGRGLTVRKQWQSGLFLSDIVWVFFSMIYFRLFMQ
                     YFPIDGFYRDFSAFAFRGGLCFVRDVACRDVRIVRSNMGEDDV"
     gene            35252..35941
                     /locus_tag="ACFI8B_000538"
     CDS             35252..35941
                     /locus_tag="ACFI8B_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3833827.1"
                     /translation="MQADLAYAAERITHDYPEQTAPKKAQLSTVSDYFRNIRTHSIHP
                     RVSVGYDFGGWRIAADYARYRKWNNSKYSVSIKELGRNDNSTSNSSHLNIKTQKTEHQ
                     ENGTFHATSSLGLSAIYDFKLNDKFKPYIGVRVAYGHVKHQVRSVESETTTVTTHNGA
                     PVPQGPTPKPAYHKSRSISSLGFGAVAGVGIDITPKLTLDAGYRYHNWGRLENTRFKT
                     HEASLGVRYRF"
     gene            35955..36161
                     /locus_tag="ACFI8B_000539"
     CDS             35955..36161
                     /locus_tag="ACFI8B_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833828.1"
                     /translation="MPSEPSDGIFDAPAVYGRGAGAGKYPYRHCRYTVTLKPAIPDNT
                     VISKPVIPAQAGIRTPDAAGIYRK"
     gene            36570..39338
                     /gene="tdfH"
                     /locus_tag="ACFI8B_000540"
     CDS             36570..39338
                     /gene="tdfH"
                     /locus_tag="ACFI8B_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC3833829.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEGHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
     gene            39369..39584
                     /locus_tag="ACFI8B_000541"
     CDS             39369..39584
                     /locus_tag="ACFI8B_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833830.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
BASE COUNT         9980 a        10747 c         9870 g         9284 t
ORIGIN      
        1 ccctttgggc cggggcgggg caacgccgta ccggtttttg ttaattcact atatatagaa
       61 ggtatatatc ctagtaggcg taatggcagc tattacaaaa tgtctgcatc aggttcacga
      121 aacgcaagtc gaaagcgtca aacaactgat tgcccgtttt gaccggctga ttgacgaatc
      181 ggacaaacaa atcgacaacc acacccgcac gcattttgac ggcaaagccc aagtggcgga
      241 gcaaatcaaa ggcatcggtt cgataacgac ggctacgctg atggcgatgc tgcccgaatt
      301 ggggcggctg tcgcacaaac ggatagcggg tttggtcggc attgccccgc acccgaggga
      361 gagcggggaa accaaattca aaagccgctg cttcggcgga aggtctgcgg tgcgtaaggc
      421 gctgtatatg gctgccgcgg cagcgacacg ttttgaaccg cttattcggg atttccacca
      481 acgcctgctg tatagcgaat taacaaaaac cggtacggcg ttgccccgcc ccggcccaaa
      541 gggaacgatt ccctaaggtg atggagcacc gggcggatcg gttccgtacc atttgtaccg
      601 tctgcggccc gccgccttgt cctgattttt gttaatccgc tatggtttaa agctttgttt
      661 cttaagtccg cagagtatgc catggttaaa ccttcaacgt cgagtgttgt actattttgt
      721 ttttaatcca ctatattctg actgcctcaa agggcaaaaa taatgtgagt atgttgaggc
      781 tgcctctttt tgaaatgaca aacaaaatgc cgtctgaaac ccgatttccg gtttcagacg
      841 gcatttgatg ttcaatcagt tgtcaaacct gattatgcca aaccttttgc tttcaacact
      901 tccagcatcg tcgtgcccaa ctcggcaggg ctgcgggtgt aggcgatacc ggctttttca
      961 aacgcggcga atttttcttc ggcagtacct ttaccgccgg agataatcgc accggcgtgg
     1021 cccatgcgtt tgcctttagg agcggtaaca ccggcgatgt aaccgacaac aggtttggtt
     1081 acgttggatt ggatgtattc ggctgcttct tcttccgcag taccaccgat ttcaccaatc
     1141 atgatgatgg catcggtatc tgggtcttct tggaagagtt tcagtgcgtc gatttggttc
     1201 ataccaggaa tcgggtcgcc gccgataccg atacaggttg attgacccaa gcccaggttg
     1261 gtggtttgtg ccacggcttc gtaagtcaat gtaccggaac gggaaataat accgatgcgg
     1321 cccggagtgt ggatgtggcc cggcataatg ccgattttgc actcgcccgg agtaatcacg
     1381 cccgggcagt tagggccgac caagcgggta ccgttgccgt tggtttccaa gtaacgtttg
     1441 gctttgagca tgtccagagt tggaacgcct tcggtaatca caacgaccaa gcctacgcct
     1501 gaatcaacgg cttcaacgat agaatccaac acaaacggag cgggaacgta aatcacggat
     1561 gcgtcagcgc cggtttcttt aacggcttct ttcatggtgt tgaatacagg caggtctagg
     1621 tgggtttgac cgcctttgcc tggggtaacg ccgccgacaa ctttagtgcc gtaagccaga
     1681 gcttgttcgg agtggaaagt accgttttta ccggtgaaac cttgaaccaa tactttagtg
     1741 tctttattaa tcaatacgct cattcttttc tccttaggcg tttacggctg caacaatttt
     1801 ttcggctgcg tcattcagac cgtctgcaga agtcagtttc agacctgatt cgttcaggat
     1861 tttcgcgccg agttcggcgt tgttgccttc caaacgaaca acgacaggaa cgttgacgtt
     1921 gatttcttta acggctgcca cgatggcttc cgcaatcatg tcgcaacgta cgataccgcc
     1981 gaagatgttg atcaatacgc ctttaacgga tttgtcttcc aaaatcagtt tgaacgcttc
     2041 aaccacgcgg tctttggttg cgccgccgcc aacgtccaag aagttggcag gttggccgcc
     2101 tttgagtttg atgatgtcca tggtggccat cgccaaaccg gcaccgttaa ccatacagcc
     2161 gatattacct tccagggcaa catagttcag gtcgaattca gaagctttca gttcacgttc
     2221 gttttcttga gatttgtcgc gcaattcagc gatttttggc aggcggtaga gcgcgttgct
     2281 gtcgatgccg attttgccgt ccacgcaagc cagtgcgccg ttttcgcgaa ctgccagcgg
     2341 gttgacttca aacagggcga agtcgttttc gacaaacgct ttgtacgcgc cggtcatcag
     2401 tttggcgaac tcgttgattt gtttgtcttt caagcccagt tggaaggcaa cttcgcgagc
     2461 ttggcaaggt tgcaggccga ccagcggatc aacggttact ttgaagattt tttctggagt
     2521 ttcagcagca actttttcga tttccacgcc gccttcggta gaagccatga atgtaacgcg
     2581 gcgggtagaa cggtcaacca ctgcgcccaa gtacagctcg gtttgaacag ggtacatgtc
     2641 ttcgcaaacc aaaacgctgt tgacaggttg gccgttggca tcggtttggt aagttaccaa
     2701 gttggtgcca atcaggcttt cagccacttc tttggctttt tcgcggcttt taacgacttt
     2761 tacgccgccc gctttaccgc ggccgccggc gtgtacctgt gctttgacaa cagcgaattt
     2821 gccgcccaat ttgtcgtaag ctgcagcggc ttcttcgccg ttgtgcgcca aaataccgcc
     2881 ttgtacgggc aaaccgtagc tagccagcag ttctttagcc tgatactcgt gtaaattcat
     2941 ggatttctcc ttaaggtgag tgggttttag gaatcgccct gcggccaatc ccatattttc
     3001 agacggtcct actatttact gaagggcaac cacatccgcg ccagcagata catgactgca
     3061 ccgcctcccc cgagaataaa aaagacgatg ccttttttac gcgaattcgg acagagcaga
     3121 taaacggcaa ggctgaaact gataaacaac aatgccacgc gccaaaccaa ttctttatct
     3181 ttgaaacggt tgagcgtata agtttcggtc atcatcacat acatctgcca caatgcagcc
     3241 atacacatgc tgatgatgcc catcggtata aagaaaatac cgactatttc tttgttcata
     3301 atgaatgtaa atgctgtttg tctgacggaa ttataaatca gaaaacgaag ctctcggaac
     3361 attcaatttg ccggaggcct taaggccgtc tgaaaatttc agacggcctt aatgtctatc
     3421 aaccgtgcaa agcgcgtttg tcggccgcca atgcagcttc gtgaaccact tcggacaagg
     3481 ttgggtgggc atggataata cgggcgatat cttcgctgct ggcgaagaat tcgagcgcag
     3541 tcacgccttc ggtaaccaat tcgctgacaa ccgggccgat catgtgtacg cccaagatac
     3601 ggtcggtttt ggcatctgcc aacactttaa ccgtaccttt ggctttgccc attgccaatg
     3661 cacggccgtt tgcaccgaaa cctgaagtac cttttttgta ctccacgcct tcggctttga
     3721 gctgctcttc ggttttaccc acccaagcga tttcagggtc ggtgtaaatc acaaacggta
     3781 cgttgttgaa gtcgatatgc ggtttttgac cggcaatgcg ctcggcaacg gcaacgcctt
     3841 cgtcgctggc tttgtgtgcc aacatcgggc cgcgaaccac gtcgccgatt gcccatacgt
     3901 taggcaggtt ggtacggcat tcgccatcca ctttgataaa gccgcgctcg tctttttcca
     3961 agcctacggc ttcagcgttc aggcctttgg tgtttggaat acggccgatg gcaacgatca
     4021 gtttgtcgaa tacttcggtt ttggcttcgc cggcagcagt ttcgtaagca acggaaacac
     4081 ctttgccttc agacttgatg tcgccgattt tcacacccaa ttcgatgctc agaccttgct
     4141 ctttggtgaa gtatttgaag gcttctttgg cgatttgttg gtcggcggca gccaggaagg
     4201 tcggcgcggc ttcaagaatg gtaacttccg cacccacgcg gttccatacg gaacccattt
     4261 ccaaaccaat cacaccggaa ccgattacgc cgagtttggc aggtacttcg gtcaggttca
     4321 atgcgccttc gttgtccaat acgttcacgt tgtcgatagc gacttgcggc aacggacgcg
     4381 gtacggaacc ggttgctacg atgacgtgtt tggcttcgat aacggttttc tcgcctttgt
     4441 tatcgacttc aatttggtaa gcatcgccat ttttaccggc aaaggaagca gtaccgaaca
     4501 ggctggttac tttgtttttt tggaacagga atttcacgcc gccggtcagt ttggtcacga
     4561 tggcatcttt gcgctcaatc attttggcca cgtcaaattt tacgtcgccg acagtgatac
     4621 cgtgttcggc aaaatcgtgt tgcgcagcgt ggaaatgttc gctggattgc aacagggctt
     4681 tagaagggat acagcctacg ttcaagcaag taccgcccaa tgcaggggca ttgcctgctt
     4741 tgttaacgcc tgcatcgaca caggcagttt tgaaacccag ttgtgcggca cggatggcgg
     4801 caacgtatcc gcccggacct gcaccaatca ctactacatc atattgagac atcgtgtcat
     4861 cctttgcttt tagcagtatc tcaaaataaa aacgatactt ttcgtttctt tttttatttt
     4921 gaatgactgc gttttgatgg ttttaagaaa aagccgtctg aaagtccata tctgccgatt
     4981 cattcgaatc aaatgtttca gacggcctca atcgttcgtt gagcctattg tagcacgata
     5041 ctacattacg ctacatctaa ataaaaattc agacggcctt ttcattaaca aataaaaagc
     5101 cgtctgaaac gattacagat ccaacaacag gcggaccggg tcttccaacg cgtctttaat
     5161 ggctaccaag gtcaatacag cttcgcggcc gtcaatgata cggtggtcgt aagacagagc
     5221 cagatacatc attggacgga caacaacttg gccgttttca accacagcgc gctctttagt
     5281 ggcatgcata cccaaaatcg cagattgagg cgggttgatg atcggggtgg acatcataga
     5341 accgaaagta ccgccgttgg taatactgaa tgtaccgccg gtcaggtctt cgatagcgat
     5401 tttgccgtct ttggcttttt tcgcgtaatc aacaattgct tgttcgatgt cggcaatgct
     5461 catttggtcg gcatcgcgca ggattggtac aaccaaaccg cgtgggctgc caattgcgat
     5521 accgatgtcg aagtagccgt ggtacacaat gtctttgccg tcaacagaag cattcacaac
     5581 cgggtatttt ttcagggcgg caacagcggc tttaacgaag aaggacataa agcccagttt
     5641 tacgccgtgt tctttctcga atttttcttt gtacttcgca cgcaagtcca tgatcggttt
     5701 catgttgact tcgttgaatg tagtcagaat ggcgttttct tgttgagaag ccaagaggcg
     5761 ttctgcaaca cgggcacgca ggcggctcat tggtacgcgt tcttcaggac gtgcgccggc
     5821 aggaagtgca acggcagggg ccacggctgc ggcaggtttg gcagcggcat tttgtacgtc
     5881 ttctttcaat acgcgaccgt cacggccgga accttgcaat acgttcacgt caacaccggt
     5941 ctcggcagcc agttttgcag cggcaggcat ggcggcgttg ttttgtgcag cagcaggaac
     6001 ggcagccggg gcagcttcgg caggagcggc tgcaggtgct tcagcagcga cagtagcagc
     6061 tgtatcgata cgggccaaaa cttggtcggc aacaacggtt tcaccgtctt gcgctacgat
     6121 ttcaaccaat acgccggctt gtggagaagg tacttccaaa accactttgt ccgtttcgat
     6181 atcgatcagg atttcgtcac gggcaactgc ttcgccaact tttttcttcc attccaagag
     6241 tgtgccttca gatacgcttt cagacagcat aggtactttt acatcaataa tcattttgtg
     6301 tctccaatgg ccctttcaga cggcctgttg tgtgttttta attctgcata tgggcattcc
     6361 gcctgagcag actcagacgg aatgctactt ggtttataac gccaaagcgt cttcaaccaa
     6421 ttgtttcaat tgagcaatgt gtttgcttga gtagcccact gcaggcgatg cgctgcttgg
     6481 acggccgaca taagacagtt tttgctcttc gctgataacg tcttcgatgc ggtggcggat
     6541 ttggtagaac gcgccttggt ttttcggctc ttcttgcgcc caaaccacag attttgcgtt
     6601 cggatatttc gccagttcgg ctttaacctc gtcgtatggg aacggataca gctgctcaac
     6661 gcggacgata gcgacatcat cttccagttt acgttcggct cgaccggctt ccaagtcata
     6721 gtaaacctga ccggcacaca ataccacgcg tttcacgctg tcgttgcttg cgcgttcggc
     6781 ggtatcacca ataaccggac ggaaagtcga accttcggtg aagttttcca gcgggctcat
     6841 tgcacctttg aagcgcaaca ggcgtttgga catgaaaatc accagcggtt tgcggtatga
     6901 acccaagact tggcgttgca agagatggaa catttgcgac gcttcagacg gcatgatgac
     6961 ttgcatattg ttctcagaac acagttgcaa ccaacgttct acgcgtgcag aagagtgttc
     7021 agggccttga ccgtcgtagc cgtgcggcag gatggtggtc agaccgcaca agcgacccca
     7081 cttggtttcg cctgaagaca ggaattgatc aatagttact tgcgcgccgt tggcgaagtc
     7141 accgaattga gcttcccaaa tggtcagctt gtcaggagcg gagcaggcaa agccgtactc
     7201 gaacgccatc acggcttctt cgttcaagat agagtcgata accaggaact cgcccaagcc
     7261 ttcgcccatg ttgcgaagag gaacataagt accgtcgtcc catttttcgc gtttttgatc
     7321 gtgcagaacg gcatgacggt gtgagaatgt accgcggccg gagtcctcac cggagatacg
     7381 cacgccatga cctttggtca gcaggcttgc gtatgcgagg gtttcggcca taccccagtc
     7441 gatggcttgt ttgccggatg ccatggcttt acgcgcttcg atcacacgtt ttgcagtcgg
     7501 atgcagggca aagccttccg gcacagcggt gaatttctca gtaagacgct caatgtcggc
     7561 ggcaggcaaa ccggtttcga ttttttcgcg ccaatcttta ccttggtatt tgctccagtc
     7621 gatttgtgtg cgttggaagt tgctcaacgt tgtttgctca acatgttcgc ctttgtccaa
     7681 agcatcacgg taggcttgga tgtaaccgtc ggcctcaact tgagttacca cgccttcggc
     7741 aatcagttgc tcggtgtaca aagcacgcgc acccggatgt tgcgatactt ttttgtacat
     7801 catcggttgg gtcaaagtcg gatcatcgcc ctcgttgtga ccccatttac ggtagcagac
     7861 aacgtcgatt acgatgtctt tatggaattt tttgcggtaa tccaaagcgg cttgaatggc
     7921 aaagcaaacg cgttcgggat catcgccgtt cacatggata accggagcgg aaaccatttt
     7981 tgcgatatcg gtacagtgta cggttgaacg ggtatcgcgg atatcggaag tggtaaagcc
     8041 gatttggttg ttgatgacga tatggaccgt accgcctgtg gtataaccgc gcgttttaga
     8101 caggttgaat gttgcttggt tgacacccaa accgataaat gcggaatcgc cgtgaatcaa
     8161 taccggcaag actttatcgc ggccgttttc gcccaaacgt ttttgtttgg cgcgtgcaga
     8221 accttccact accggattga cgatttccaa gtgtgacggg ttgaacgcca aagaaacgtg
     8281 catcgggccg tgaggggtgg caatatcgga gctgaagccc atatggtatt tcacgtcgcc
     8341 actaggcagt ttgatttcgg cacggccttc aaattcggca aacaaatcgc cgggtttttt
     8401 gcccaaaatg ttcaccaaaa cattcagacg gccacggtgc gccataccga tgatgacttc
     8461 ttccacgccg tccttaccgg cgttttgaat caggtagttc aaaccggcaa tcgcgctttc
     8521 gccgccttcg acaccgaaac gtttctgacc gacatatttg gtatgcagat aacgttccaa
     8581 agtctcggca gcggtcatct ctttcaagat acggcgtttt tgatcggcat tgtaatgcgg
     8641 tgtggacaat acgctttcaa aatagttgcg tacccaacgg cgctcttcgg tattgggaat
     8701 atagatatat tccaatgcga tgtggccgca gtaggtttgt ttgaggttgc tgatgatttg
     8761 ggacaagggc agtttgccgc gattggcaaa atcaccctcg cccatattga atcgaagcgc
     8821 catatcggca tctgacagac cgtggaattt cggatcgagg gcttcaatat cgcgcggagg
     8881 gatacgtttg agcggatcaa gttgggccgc acccacgcct tggatacgat aggcagaaat
     8941 cagccgtaaa acgctgactt gctttttcag cattgcctca tccgcaccgc ccgcaacggc
     9001 agatgcaatt ttctttttcg ccaaagtaac aaatgattcg cgaatcggtg tgtgtgcgac
     9061 atcgacagca accgtccccg gctgtttgct caaatcggta aaatactgct tccatttttc
     9121 atcaaccgca tcggggtttt ccaaaaaagc ctcgtacaat tcctcaatgt agggtgcgtt
     9181 cgaaccgaac agataagaga aattgagttt ttcgtccatc atggcgtgcg ctcttttctt
     9241 taaaatataa acaaagggta gaagtccgcg tcctgacggc tgaactgatg ccgtctgaaa
     9301 agacatttta ttctacccta ttttaaaccg gattgcttgt cttgatttca tccgactaca
     9361 atcagacgga ttcaggaaac aatccggttc tctgatattg cctgtttgac aatatattaa
     9421 tgtatgttac ctctcgcctg ccggcacata atcgcggcgt tccgaaccgg tataaagctg
     9481 gcgcgggcgg ccgattttca gcgaaggatc gctaatcatc tcgtgccagt gcgaaatcca
     9541 gcccacgctg cgcgacaggg cgaagatgac ggtaaacatt tcggtcggga tgcccagcgc
     9601 ggacaggacg atgccggaat agaaatcgac attgggatac agtttgcgtt cgataaagaa
     9661 cgggtctttc agcgcaatct gttccaattc catcgccagt ttgaatttcg gactgtcttc
     9721 caagcccaat tccttcaaaa cttcatagca ggtttcgcgc ataatgctgg cacgcggatc
     9781 catattgcgg tatacccggt gtccgaagcc catcaggcgg tatttgcgct gtttcacgcc
     9841 ttccatgtat gcggcaacat tggacacatc gccgatttca tccagcattt tcaacacggc
     9901 ttcgttcgca ccgccgtgcg aagcacccca caggcaggcg ataccggcag caatacaggc
     9961 aaacgggttc gcacccgaag agcctgccag acggacggtt gaagttgagg cgttttgctc
    10021 gtggtcggca tgcaaaataa agatgcggtc gagcgcgcgt gccaaaacgg gattaggttt
    10081 gtagtcttca cacggtgtgg caaacatcat atgaaggaag ttttctgaat aagaaagatt
    10141 attcttcgga taattgaacg gcagaccgtt tgaatagcgg taacacattg ccgcaatggt
    10201 cgggattttg gaaataaggc ggtaaatcgc gattttgcgg tgttcgggat tagtaatgtc
    10261 caagctgtct tggtagaacg cagacagcgc gccgaccacg ccgaccatca tcgccatcgg
    10321 atgcgcgtcg cggcggaaac cccggaagaa ccaagtcagc tgttcatgca ccatcgtgtg
    10381 gcggcggact gtattgtcaa attctgcctt ttgctcggga gtcggcagtt cgccgtaaat
    10441 caacaggtag cagacttcca aataatcgga cttttcggcc agctgctcga tggggtatcc
    10501 gcgataataa agcaagcctt gatcgccgtc gatgtaagta attttagact cacagcttgc
    10561 ggttgaaaca aatccggggt cgaaggcaaa caaacctgta ttttttgtca gcccccgaat
    10621 gtcaaccaca tcatgcccga tgctggcttc caataccggc agctccaaac ctgcccgacc
    10681 cggtacgttg agtttgattg atttggacat attatcgctc ctttgcaagt tataacccac
    10741 gttactgcat ccgcagcacg ttctttgctt tcagacggcc tttgcatttt gaaatctgcc
    10801 ctcagacaaa tttcagatgc ctcatgcccg tctgattttt tcaagcatcg ggataaggtg
    10861 ccctttgtcc gtttccgaat gcccgtgaat caaggcaagc aattcttgat cttgaaattc
    10921 aaggatttcg gaaaactcgg acagctcttt atcgctcaaa tgctcgaatt ctttttccat
    10981 aaacctgccg aagattaaat ctagttccaa caatccccgg cgggtttgaa aacggatttt
    11041 ccgtttggca atgtcgtcaa aaaccatcat ttcttaaaca acccgtttca acataatctc
    11101 tttaatctta ccgatggccc gggtcggatt caagtgttta ggacatacgt ccacgcagtt
    11161 cataatggtg tggcagcgga acaaacggta cggatcgttc aaattatcca aacgctcatt
    11221 agtgatggta tctcggctgt ccgcaatgaa gcggtaggcg ttcagcaagc cggacggacc
    11281 gacgaatttg tcgggattcc accagaatga cggacaggcg gtcgaacagc aggcgcacaa
    11341 aatacattcg tacaaaccat ccaactcttt gcgctcttcc tgagtttgca gacgctcttt
    11401 gtccgcatca atcggattgt cgttaacaac ataaggtttg acggaatggt attgtttgaa
    11461 gaactgggtc atatccacaa tcaggtcgcg gatgacgggc agaccgggca gcgggcggat
    11521 tttgacaggc tgtttcaagc tgcgtaaatc ggtcagacac gccaagccgt ttttgccgtt
    11581 gatgttcata ccgtccgatc cgcaaatccc ttcgcggcag gagcggcgga aagacaaggt
    11641 atcgtcttgt gctttcaggc gtaccaaagc gtccaaaagt ttcacgtcgg tcggttccaa
    11701 ttccaactcg taacgctgca tataaggctt ggcatcaacg tccgggttgt aacggtaaat
    11761 ttcaaaactc attttttcca tgagaagtgt tccttttctc atcaactgcc caatgatttt
    11821 gtattataaa ttcaagcagt tcaatgtggg tatggttagg tttcagacga cctttgaggc
    11881 gaagaccgtc tgaaaacgca tcaataaacg cgtttggccg gtttgatgta ttccacgctc
    11941 aaaggcttgg tgtgtaccgg tttgtaagac aaggtattgg tgtccgaatg atacagggtg
    12001 tgtttcatcc aattttcgtc atcgcgctca ggatggtcgt ctgaagcgtg cgcaccgcgt
    12061 gattctttac gtgcttcggc agacaccaaa gtcgctttcg ccacttcaat caggttatcc
    12121 aattccaaag cttcgatacg cgcggtattc cacactttgc tcttgtcttt gatttcggta
    12181 cgtttcacac gctcggcaat cgccatgatt tcttgaacgc ctttgctcag aatctcatcg
    12241 gtacggaaca cgccggcgtg cagttgtacg gagcgttgca gttcgcgacg caatgcatca
    12301 acgttttcac catcggtttg actgtccaaa cgctcgatac gttggcgggt caactcaccg
    12361 gcattagcag gcagaggttt ccaatcgctt tgctctttga tgaatttaat catgctgtca
    12421 ccggcagctt tgccgaacac caccaagtcc agcagggagt tcgtacccaa acggttcgca
    12481 ccgtgtacgg aagcacaggc gcactcacct gcggcataca ggccttttac aggtacttcg
    12541 tactcgtcgc cttgcggaac aacaacttca ccgtgataat tggtcggaat gccgcccatc
    12601 atatagtggg tagtcggcac aaccggaatc gggtctttaa tcggatcgat accggcaaac
    12661 tgaatggaaa tctcgcggat gcccggcagt ttttccataa ttttttctgc accgatatgg
    12721 tcgattttca gtaagacgtg gtctttgttt ttaccacagc cgcgaccttc atagatttcc
    12781 atcgccatcg cgcgtgaaac cacgtcgcga gaagccaagt cttttacggt cggcgcatag
    12841 cgttccataa agcgttcgcc gtcggcgttc aacagaatac cgccctcgcc gcgtacgcct
    12901 tcggtaatca acacgcccgc acccgccacg ccggtcgggt ggaattgcca gaattccata
    12961 tcttccaacg gaatgcccgc acgggcgcaa atgcccaaac cgtcaccggt attcatataa
    13021 gcattggtag aagaagcata aatacgaccg ccgccaccgg tagcaaacat cacggccttg
    13081 gcgtggaaaa tataaacttc gcccgtttcc atttccatgg cggttacgcc gacgacatcg
    13141 ccgttttcat cacgaatcaa atcttgcgcc gtccattcca caaagaattg tgtattggca
    13201 cggacgtttt gttggtacaa agtatgcaac atcgcatgac cggtacggtc ggcaaccgca
    13261 catgcacgtt ctaccgcacg tttaccatgt tcggcagtat gtccgccgaa aggacgctga
    13321 taaattttgc cgctttcaac gcggtcaaaa ggcataccca tgtgttccaa ctcaatcacc
    13381 gcttcaggcg cagcgcgaca cataaactca atcgcatctt ggtcgcccag ccagtcggaa
    13441 cctttcacgg tatcgtacat gtgccagtcc caacggtcct cctgcacatt acccagagag
    13501 gcggaaatac cgccctgcgc cgctacggta tgcgagcggg tcgggaacac tttagacaaa
    13561 acggcacaat tcaaaccgga tttggataat tggagggctg cacgtaaacc tgcaccgcca
    13621 ccgccgacaa tcacggcatc aaacttgcga acaggaaaac ccatacttac ccccaaatca
    13681 ctttaactga atacacgagg cagccgacca gccagacaat ggtggcaacc tgcaaaaaca
    13741 aacgcacgcc gaagggtttg atatagtcca tccacaaatc gcggataccc acccaagcgt
    13801 gcaagaatac ggcgataaag ctcacttggg taaatacttt tacccaagct tgactaaaaa
    13861 atgcctgcca tgccggatat tctttaggca gggcaaatag aaccactaaa agtgcaacgg
    13921 tataaatcaa cataataacc gcagtcgcac gctgcattac ccaatcgcgc aaaccgtaat
    13981 gggcaccggt caatttacgt tctaccataa caacgctccc aaaacgacag tcaaaaccaa
    14041 tgcggaagca aatacggctt tagcggtatt gcgcgcagta ttcagctcaa ggcctttgtg
    14101 cgcatccaaa aataaaaagc ggataccggc gagagaatgg tgcagataag cccacaatat
    14161 accgattaaa accagcttga ccaaaggatg ggaaacaatg gcacggtaag tttcaaatgc
    14221 agactcttga ctcagggtac cggacagaaa atacagcagg aaaggcagca taataaacag
    14281 cccgaccccg ctaatgcggt gaaggatgga aactatcccg ggtatcggca gacggatgtt
    14341 cggcaaatcc agataaacag gacgcagttt gacagacata gtcaattcct ctgtagttat
    14401 atatcgactc aaaaaccgta aacccattgc tacactgggt tacatcaacg ttaatttacc
    14461 tgttttatcc cattttgaac agtgtatttt aaaaacaaac tgatagattt tgacaaacac
    14521 gcgtggaaac agcagattaa aagaaagatt tttagttttg ccgctccctt cccgacacgg
    14581 tatcagcatc cgacccccaa ccccatcccc aaaaataaaa tgccgtctga aaaaaatttc
    14641 aggcggcatt gttttgccat atcggtcagg ctttcaattt cgcatcggca tcgcgcaacg
    14701 cggtacgcag accttcctcg ataacaggat ggtagaacgg catatccagc atttgcggaa
    14761 cggtcatctt catttgatgc gcccatgcca acaagtgagc caaatgttcg gcggcagggc
    14821 ctacgatttc cgcgccgata aagcggccgg tggctttttc ggcatacagg cgcatatggc
    14881 ctttgtttac cagcatcacg cggctgcgac cttgattttt gaacgatact tcgccgatga
    14941 caaattcgtc ggcttggtat tgcgcggcaa cctgcgcgta tttcaaaccg acaaagccga
    15001 tttgcggact ggtaaacacc acgccaatgg tgctgcggcg caaaccgctg ccgatattcg
    15061 ggtagcggcc cgcgttatcg ccggcaatct taccttggtc ggcggcttca tgcagcaggg
    15121 gcagttggtt ggacgcgtcg cccgcaataa agatatgcgg aatgctggtc tgcatggtca
    15181 gcggatcggc aacgggtacg ccgcgcgcgt ctttgtcgat attgatgttt tccaaaccga
    15241 tattgtcaac gttcggacgg cgacctacgg ctgccaacat atattcggca acaaatacgc
    15301 ctttttcgcc atcctgctcc caatggactt ctacattgcc gtctgcgtcg agtttgacct
    15361 cggttttagc atccagatgc agtttcaatt cttctccgaa cacggctttc gcctcgtctg
    15421 aaacaacggg gtcggaaatg ccgccgatga ttccgcccaa accgaaaatt tcaactttca
    15481 cacccaaacg gtgcaatgcc tgacccagtt ccaaaccgat aacgcccggc ccgaacacgg
    15541 caacgcgctt aggcagcgta tcccacgaga aaacgtcgtc gttgataatc aaacggtcgc
    15601 ccaaagactg ccactgcggc aggatgacgg gacgcgaacc ggtagcaatc acgaaacttt
    15661 ttgccgcaat ttggatatgg tcgtcgattt ggacggtatg ctcgtcgatg aatttagccg
    15721 agcccatgat gcgcttgtcg gcaggccatt cttccacatc ggtaacgaca aagccgacaa
    15781 aacggtcgcg ctcggatttg acgcgccgca taacctcttc tccgttgacg acgatgctgt
    15841 ctttgtccaa atgcacgccg aaggggtcgg tatgcaatgc gtggtgacgc gcttctgcgg
    15901 cggcaatcaa gagtttggaa ggcatacagc ccacgcgcgc gcaggttgtg ccgaacacat
    15961 tgttttcaat caggtaaaca ttatccgaat gtaaacgggc attgcgaaac gcacccatac
    16021 cggcagtacc gccgccgatt acgacgatat ccgcttgaat ttttttcata atctttgtcc
    16081 ttccgttttc gtctgaaaca ttgccgacac agaacatatc ggtttcagac agcatttaaa
    16141 tatcttccca atcaaaaaca ttattcagat aaataaaaac aaaatttcga ttgagtgcga
    16201 caatatccaa atcttcttgt aaatatagag ttattcggag cggcagtata gaaccgcccg
    16261 aataactcta tccggtatat cagacagttt aatcaaactg ccttaagcaa acagctatca
    16321 gtttttagcc aagtaagctt ccaaatcttc gctgccgccg atgtatttgc cgccgatgaa
    16381 gacttgaggg gcagtcatct tgccggtaat agcgcgaacg gaagtaacgg ttgcatcttt
    16441 gcccaatacg atttcttcgt aagacaaacc tttgtcttgc aaagcttgtt tgactttggc
    16501 acagaattgg caacctggtt tagtgaaaat tgccacagac tcttgagcct tccaatcggg
    16561 agcaacgaat ttcagcatag tatctgcatc ggatactttg aaaggatcgc ccggttcttc
    16621 aggttcgatg aacatttttt caaccacgcc gtcgttaacc agcatggagt aacgccaaga
    16681 gcgtttgccg aagcccaagt cttctttacc gaccagcata cccatacctt cggtaaattc
    16741 gccgttgccg tcaggaatca tgtagatgtt gtctgattct tcttcggcag cccaagcgtt
    16801 cattacgaaa gtatcgttta cagatacgca gcagattgcg tcaacgccgt tttctttgaa
    16861 tgcgccgaac aattcgttgt aacgcggcag gtgtgaagaa gaacaagtcg gggtaaatgc
    16921 accgggcagg gagaatacga ctactttttt gcctttgaac aaatcatcgg tagacacatc
    16981 tttccaagtg tcgccgacgc gggtgcggaa tactacggaa ggtacttttt gaccggtacg
    17041 atcttgcaaa gccattttga gctccttaat agattggttt gagaaataat tgtgaatcgc
    17101 cgcgcattct accccatgca aaatgatttg tataatttat agtttaaata atataaatta
    17161 tttaaaacta tcaaatacaa ctccgatttg cctgttccca acatataaag ccgaattaga
    17221 gaaaaacaag ctgcaatgaa aagattttcc ggcaactgct cccctgcgcc gcaactcagg
    17281 acaaaatgcc gtctgaaagg ctttcagacg gcatttgttc atacggtctc ggcttatttc
    17341 gccaattcgg cacgcagttt gcgggttacg ttcatcatta cttgaagctg ttccagagtt
    17401 tctttccagc cgcgtgtttt caggccgcag tccgggttaa cccacagacg ctcaaccgga
    17461 acaacctcaa tggctttgcg caacaggtgt tccacttcgg cttctgtcgg tacgcgcggg
    17521 ctgtggatgt cgtaaacgcc cgggccgatg tcgttcgggt atttgaattc gccgaacgca
    17581 gccaagagtt ccatgtcgga acgtgaagtc tcaatggtga tcacgtccgc atccattgca
    17641 gcaatcgcag gcaggatgtc gttgaactca gagtagcaca tatgagtgtg gatttgagtg
    17701 ctgtcttcgc aacctgcaga ggataggcgg aaggattcgc ccgcccagtt caggtaggca
    17761 tcccaatcgg cacgtttcaa aggaagacct tcgcggatgg caggttcgtc aatttggatg
    17821 actttaatgc cggctttttc cagatccaat acttcgtcgt tcagagccag tgcgatttgt
    17881 ttgcacacgg tagcgcgcgg aatatcgttg cggacgaaag accattgcag aatggttaca
    17941 ggaccggtca acataccttt catcgggcgt ttggtcaggt tttgtgcgta agtagaccaa
    18001 gcgacagtca tagcttcagg acggcttacg tcaccgaaga tgataggtgg tttaacgcag
    18061 cgtgagccgt agctttgcac ccagccgtat tgggtgaatg caaaaccgct caacagttcg
    18121 ccgaagtatt cgaccatgtc gttacgctcg gcttcgccgt gcaccagtac gtccaagtcc
    18181 agtttttctt gctcttcaac caccaaggcg atttcttttt tcattgcggc ttcgtaatcg
    18241 gaggcagaca gttcgccttt tttgaaggct gcgcgtgctt ggcggatttc ggtagtttgc
    18301 gggaaagaac caatgttggt cgtcggcagc agaggcaggt tcaaccatgc ttgttgcgct
    18361 ttgatacggt cggcaaacgg tgatttgcgt tggcctgcgt tgacaggcag gtcggccaag
    18421 cgtttggcaa cgtctgcacg gtggatttca ctgctgttgg cacgggagtc ggcagcagct
    18481 tggctggcgg ccagttcttc ggcaacagaa tcacggcctt cattcaatgc ggctttcaga
    18541 acacgcaatt cttgggtttt ttgcagagtg aatgccaacc aagagtacag gtcgggtttg
    18601 ttggctttca gtttttcttc gactgacaag tcaaacggag tgtgcagcag cgagcaagag
    18661 ctggaaatcc acaaacgctc gcccagtttg gcttgcagag gcccgacggt ttccaaaact
    18721 ttgttcaggt tggcgcgcca aatgttgcgg ccgtcaatca cgccggcaga caggactttg
    18781 tcgtagccgg cgaacgcgtc cagttgctcg ggtgcgcgta ccaagtcgat gtgcaggccg
    18841 tcaacaggca gggatttcaa caatgcggcg tgttcggcaa cagaaccgaa gtaagtgccc
    18901 aacaggattt tggcgcttac tttgttcaaa gtagcgtaaa cgtctttgta tgcttctacc
    18961 cattctttag gcagatcgac agccaaagca ggctcgtcga tttgaatcca ctcggcaccg
    19021 gcttcaacca aagcagtcag gatttcaacg taaacaggca acagtttagg caacaggctc
    19081 agacggtcga attcaacgga gcctttttct ttacccaccc acaggaaagt caaaggaccg
    19141 acaacggtcg gtttggcttt caagcccaaa gtttgggctt cttgcagttg ttgaacgtag
    19201 tgtttggcgt tggctttgaa ttcggtatcg gcatggaatt caggcaccag gtagtggtag
    19261 ttggtgtcga accatttggt catttcgatg gcgaattggt ctttgttgcc gcgcgccagt
    19321 tggaagaatt gttccaaagg caggttttgg ctatcgaagc cgaaacgggc gggaatcgcg
    19381 ccggtggcaa cttgcaggtc gaggatgtga tcgtagaaag tgaaatcacc tacagcaacg
    19441 taatcagcgt tggcagcagc ttggtgtttc cagtttttct cgcgcaggtc tttggcaacg
    19501 tcgagcagct cttgttcact gatctctttg cgccagtatt tttcttgggc gaatttcaat
    19561 tcacggaagg cgccgacacg cgggaagcct gagaaatgta atgttgtcat gttgaactct
    19621 ccagttggat tttgtaagga ccggttcaat ccgaatcggc cggtattgta aatcagaatg
    19681 gctcggatag ggcggcgttt tgcttttcag acggcctcgg atgctgtctg aagccgtttt
    19741 cggctttaag ggcgcgcgcc taaaatatgg cagatggcgt aagtcagctc gctgcggttg
    19801 agcgtgtaga agtggaaatc tttcacgcct tcgcgggaca ggactttaac catatcgatg
    19861 gcgatactgg cggcgacgag gttgcgcgtg ccttggtcgt cgtccaagcc ttcatacatt
    19921 tgcgacagcc atttcgggat tttgacgttg gttacttgtg ccattttgcc gagttgcttg
    19981 aagttggtaa caggcaggat gccgggaacg atttcgacgt cgatgcccaa catcacgcag
    20041 cggtcgcgga agcgcaggta gcgttccaca tcgaagaaga attgggtgat gacgtggttc
    20101 gcgcccgcat cgattttgcg cttcagatta atcaggtcgg cttgtgcgga tttcgcttcg
    20161 ggatgtactt cggggtatgc tgctacagag atgtcgaagt cggcgacgga gcgtaatagc
    20221 ttaaccaagt cttcggcgta aaacggtttt ttctcataac cggccggctc gtcgccacgc
    20281 agggcaacga tgcggcggat accgctgtcc caatagtctt tggcgatttg gcgcaattcg
    20341 tcgtgggatg catcgatgcc ggtcaggtgc ggagctgctt ccaaaccggt ttcctgtttg
    20401 atgcgtttga cgatgccgtg cgtgcggtcg cgttcgccgg agtttgcgcc gtaagttacg
    20461 gatacgaact tgggatgcag ggtttgcaga cggtggatgg aatcccacag catcgtttcc
    20521 atttgttcgt ttttcggcgg gaaaaattca aacgaaacgt tgatgttgcc tttcaaatcg
    20581 gaaaggctgt tgtttaacgc attgatttct tttgcataat tctgcatacc ttcgttatct
    20641 tttacatgat gtttcaggcg gtatgataag gttgcaccga atatgagtca atttcaaaat
    20701 tttcaggttt atgatgaaaa tttttaatat ttagcaaaaa ctatacgctt ttcttgtaca
    20761 atccttctaa attatgataa agtttgttat gttataacac ttcaatcaag gcaatatatg
    20821 aaaccgaata ttcatcctga caattatcga accgtccttt ttttcgacag cagtgccaat
    20881 gaaggctggc tgattcgttc ttgcgccgga acgcacggaa aaacaatggt ttggacagac
    20941 ggcaaagaat atccgctctt ttccttagat acttcctctt cctcacatcc cgtctatacc
    21001 ggcaaacagc gtaacgtcaa taccgaaggc cgtgccagca agttcaacca acgtttccaa
    21061 tccgtcatgt catcgtttag aaaggacaaa taatgcaggt tctgtcttcg ctcaaaaccg
    21121 ccaaacaacg ccaccgcgac tgccaaatcg tcagacgcag gggcaaagtg tacgtcatct
    21181 gcaaaagcaa tccgcgtttt aaatcgcgcc aacgctgacc ccctgccctt cctgcttcgg
    21241 ttgtaaagcc cctcctaccg caagcccttt tcaaatatgc cgtctgaaac agaaaaatgc
    21301 gtttcagacg gcatttttcc gtcgatacga atccggaatg tgtttgaaat acagcataaa
    21361 cagataccaa aacagcgtcg cggcggctgc accgagcagt acccatgcgc tgatttcggc
    21421 gacttcgcgc aggacacgtt tctgcctgcg gctaaagagg cggttgataa tcagcgcggc
    21481 aagtgcgaaa aagaaaaaaa tacggaaaag tctgcctatc atgtcgggaa gcgaaaagta
    21541 tggttcgggc gcggcggcat gggaaaggat gtttcagcgc gtcaggctgc ttgtccgacc
    21601 gtgtcaaaaa gtcatgccgt ttgcgatata ataacgcttt tcgccgcatt tgttggaaca
    21661 ggatatgagt caaaatacct cggtgggcat tgtaacgccc caaaaaattc cgtttgaaat
    21721 gccgctggtt ttggaaaacg gtaaaacttt gccgcgtttc gatctgatga ttgaaaccta
    21781 cggcgagctg aatgctgaaa aaaacaatgc ggttttaatc tgccacgcgc tgtcgggcaa
    21841 ccatcacgtt gcgggcaggc attcggcgga ggataaatat acgggctggt gggacaatat
    21901 ggtcggtccc ggaaaaccga ttgatacgga acgttttttc gtggtcgggt tgaacaatct
    21961 gggcggctgc gacggcagca gcgggccttt gtcgatcaat cctgaaacgg gcagggaata
    22021 cggcgcggat tttccgatgg ttacggtgaa ggactgggta aaatcacaag ccgcgcttgc
    22081 cgattatctc ggcatcgaac aatgggcggc ggttgtcggc ggcagcttgg gcggcatgca
    22141 ggctttgcag tgggcgattt cctatcccga acgtgtgcgc cacgccttgg tgattgcgtc
    22201 tgcgccgaaa ctgtccacgc aaaatatcgc gtttaatgat gtagcacgtc aggcgatttt
    22261 gaccgacccc gatttcaatg aaggacatta ccgcagccac aacaccgttc ccgcgcgcgg
    22321 tttgcggatt gcccgtatga tgggacacat tacgtatctt gccgaagacg gtttgggcaa
    22381 aaaattcgga cgcgatttgc gttccaacgg ctatcaatac ggctatagcg ttgaatttga
    22441 agtagaatcc tatctccgct atcaaggcga caaattcgtc gggcggtttg atgctaatac
    22501 atatttgctg atgaccaaag ctttggacta tttcgatccg gcggcggatt tcggcaacag
    22561 cctgacccgc gccgtgcagg atgtgcaggc aaaattcttt gtcgccagct tcagcaccga
    22621 ctggcgtttc gcgcccgaac gttcgcacga actggtcaag gcactgattg ccgcccaaaa
    22681 atccgtgcag tatatcgaag tcaagtccgc acacgggcac gatgcctttt taatggaaga
    22741 cgaagcctat atgcgcgccg taacggctta tatgaacaat gttgacaagg attgccgatt
    22801 atgaacctgc gcgatgattt gcaactgatt tacgaccgga tacccgaagg cagccgcgtc
    22861 ttagacttgg gctgcggcga cggcgaattg ctggcggctt tggcggaaca caaaaaatgc
    22921 agcggctacg gcatcgaaat cgacaccgac aacgtgattg ctgcgatgtc gcgcggcgta
    22981 aatgttatcc aagccgattt ggaagaaggg ttgaccgcat tcaacgatca aagttttgat
    23041 gtgattgttt tgagccaaac catccaagcg atgcagaata ccgaaaaaat cctgcgctgc
    23101 ctgatgcgcc tcgccaaaca ggcaatcgtc agtttcccga atttcggcta ctggcgcaac
    23161 cgcgtccaaa tcgcactcgg cggacatatg ccggtttccg aacgtatgcc ctaccattgg
    23221 tacgacacgc ccaatatcca ttggtgcacg ctcaaagact ttgatttgtt gtgcgccaaa
    23281 aacaatatcc gcgtcctcga acgcgccgtg atgacgggca acagacaggt caaacatttc
    23341 cccaaccttt tgggcagcct tgcgttttac cgcgtcgggt aagacaggtt gaaaaccttg
    23401 tcggcaataa cgggtttctc ggttctatta acaacagtcc gtctgaaaaa tgaaacggca
    23461 ggttttcaga cggccttttc aatattggaa acaatagaat gaaaaacaaa acgtcatcac
    23521 ttcccttatg gcttgccgca atcatgctgg ccgcgcgttc cccgagcaaa gaagataaaa
    23581 cgaaagaaaa cggcgcatcc gccgcttcgt cttccgcgtc ccaatccgat ttgcaaccgg
    23641 ccgcatccgc ccctgataac gtcaagcagg cagaaagcgc gccactgtga aattgcaccg
    23701 gcctgcaccc cgccgccggc attggcgatc tcatacagca aatcgccgaa cacatcgact
    23761 cggactgtct gtttgccctt tcccatcacg aactggaaac ccgtttcggc ttacccggcg
    23821 gcggctatga caacatacag cggctgctgt ttcccgacat ccgccctgaa gatcccgact
    23881 accatcagaa aatcatgctg gcaatcgaag acttgcgtta cggaacgcgc accatcagcc
    23941 ggcaggcaca agatgccata atggaacagg aacgccgcct ccgagaagcg acgctgctgc
    24001 tgacacaagg cagtcaagaa acccgcggac aaggcgagga accgaaacgc gcacgttatt
    24061 ttgaagtttc ggcaacatct gcctatttga accggcacaa caacggactc ggcggcaatt
    24121 tccaagacat cggccaattg cccggctatc tgaaaatgca cggagaaatg cttgaaaacc
    24181 aatcactctt ccggctgtcc aaccgtgaac gcaatcccga caaaccgttt ttagacatcc
    24241 attttgacga aaatggcaaa atcacgcgta ttgtcgttta cgaaaaaaat atctatagtg
    24301 gattaaattt aaatcaggac aaggcgacga agccgaagac agtacagata gtacggcaag
    24361 gcgaggcaac gctgtactgg tttaaattta atccactata cttcaatcca aacacggggc
    24421 gaatataaaa aaatgccgtc tgaaagcctt tcagacggca tttttcaagt aggatatcag
    24481 gcgcgtttgc ggaattcgcc ggtacgggta tcgatttcga ctttgtcgcc gttttcgatg
    24541 taagacatca cttggatttc ggtgccgccg accagacgcg cggttttcat cactttgccg
    24601 gaagtatcgc ctttgacggc aggctcggtg tagtcgactt cgcgcacgat gatggtgggc
    24661 agttcgacag aaatgggatt gccttcatag aaggtaactt cgcattggtc ttccataccg
    24721 tcaacgatga atttcaacgc gtcgccgatg ttgtcggctt cgatttcgta ttggttgaat
    24781 tcttcgtcca taaagacgta catcgggtcg gcaaaatagc tgtacgtaca gtttttgcgg
    24841 gacaggatga ccacgtcgaa tttgtcgtcg gctttgtaaa tggtttcgga agcagcgccg
    24901 gtcagcaggt ttttcagttt catgctgact ttggcggaag agcggccgcc tttgatgtat
    24961 tcggtttttt gaacgaccat aggatcgttg ccgaccataa atacattgcc ggcgcgcagt
    25021 tcttgtgctg ttttcattgt gattttccaa tcaaatcggt tgtcgtaaaa cgcgctattc
    25081 tagcgtattt tttgatgctt tgaaacaaag gcggcgagtt tttcggatgc ggaaggctgc
    25141 ccgaaaagat aacggctcca atcctccgcg ccttgccgcc agccgttttg atgttgttgc
    25201 agggtttgcc aacattcgag gcgttgtgtt gcggataaag cctctccgcc gttgaggtcg
    25261 tccgaaagga ggcggtgcac cgatgcggtt tcgggcgtgt agaagccgta tgccttatcc
    25321 caaaaggcgt ggagtttgtc gagatggaca ttctcgtctt gcgggtagat gtgccaaaaa
    25381 aagggttttc cggcaagctg ggtacgcacg aaactgtctt cgccgcgtat cacggcgcag
    25441 tcggcgaggt gcagcaattt gtcgaagtcc tgttgcggca cgaacgggat tttgacaagg
    25501 cggacggatg ccgtctgaaa gacaccgccg tcattttgca gggcgttttg cggaataacg
    25561 ccgctttgtt tgaggctgtc gataatttgc gcccccgcca gcagtagggt catcaggctg
    25621 cctgcctgtt gccacatgtc cagccacttt gcccaaacat cgccccgata gccgaaaagc
    25681 agccattcgg gggcgttttt ttcgggcagc accagccgcc ggcgcagggc ttcggtatcg
    25741 aaacggacgg cttcgcggta atcgcgttcg cgtatcaacc cgccgctttt ttcgctgaaa
    25801 cccataaacc aaaaatattt ttgaacgccc tcctgcggcg aaggcatcag gtgcagcctt
    25861 tcattgcttt cctccgcgct caaatattcc caattcagcc aaagcggttt gtgtcggcgg
    25921 atgatgttca gcacattttc gggcaggtcg caggcaaaag tttcgataac ggcatcggga
    25981 acgggcgcgg tatcaatgtc tgccgcatcg gaatgccaag tgcggacatg aatatcctga
    26041 tgaacgaagg gaacatcggg caaatcggga caaagcgcgc gcaaggcgga cacgtcgtcc
    26101 gtccacaaat gcacctgcca accgagttcg cggtgcaaaa cacgggcgag ccgccacgaa
    26161 acgccgatgt cgccgaaatt gtcgatgacc ttgcaaaaaa tccaacagac aggaaaagcg
    26221 tatgtattca taaccatcgg gaaaacaagc taaaacgcgc cgaaatagtg gaacaaatac
    26281 acaaccagca aaacaatcgg aataaacaga aacatggttt gacgtttcaa atcaaaccct
    26341 tgtttaagat gatgcttgat ggtttcaaag aggacgaatt taaacaaaaa cgtcgcttcg
    26401 caggcaagga tgatgccgtt gatcagatgc tggggtttgt cttcgggcgg attggcggcg
    26461 aaaacggcaa gcgtcgccaa ggggatgagg cagacggcga gccagcggac aagcaggttt
    26521 cgggacattg caaggctttc aaaaaagcaa aacgccatta tcgcctcaag aacgcacctt
    26581 gtctaacgga cgcgccggag atgccgtctg aagccttgcc gccggaaaac gccgcaccac
    26641 cgaaccttta aatatacccg ggacgggcgt tccccgagaa atcggacgct gccgccgatg
    26701 cctgcggaca aggcggcatc gggcgcgggc atattgaaag cccgacgcat cggtttgaac
    26761 gcattttata aaggcgtacg gcaaacccgc cccttgccgt cggcgtgctt ccgcaaagca
    26821 aaatataagg gcggcgcgct ttccgctata atccgcattt ttccaaacct gaaacattat
    26881 gccgaccccg aaagccttta ccctgctcgg gccgaccgcc tgcggcaaaa ccgcgctcgc
    26941 cctcaaaatt gccgaaaccc ttccggtcga aatcatcagc ctcgattctg cactgctcta
    27001 taccggtatg gacatcggca cggcgaaacc gtccgcctcc gaacgcgcct ttgtcccgca
    27061 ccaccttatc gacatcatta cgcccgttca gacatacagt gccgcccgtt ttgtcgaaga
    27121 ctgcacgcgt ctgaccggcg aaatcacggc gcgtgggaaa tgtccgctga ttgtcggcgg
    27181 cacaatgatg tatttccgcg ccctgaccca aggtttgaac gatttgcccg aagccgatgc
    27241 ctgcctgcgt gccgacttgg acgaacaaaa acagatgtac ggtttggatt tcctctaccg
    27301 caccctgcaa aaagtcgacc ctgaaacagc atgccgtctg aaaccgaacg acagccagcg
    27361 catcggacgc gcccttgaaa tctattacct gaccggcagg ccgatgagcg cgcacctgaa
    27421 cggacagccc gaacacaccc tgcccttcga actctacacc gccgccctga ttcccgaaga
    27481 ccgcgcccga ctgcacgaaa acatcgccct gcgtttccac ctgatgcttg aacagggctt
    27541 tatcggcgaa gtagaaaacc tgcgccgccg ctatcccggc ctgaccgccg actcccccgc
    27601 catccgctgc gtcggctacc gtcaggcgtg ggaacacctc gacggtgcaa ccgacagaca
    27661 aaccttcatc gaaaaaggca ttgccgccac gcgccaactt gccaaacgcc aactaacttg
    27721 gttacgcaaa acacctttag actgcgttgc cgacccgttt tcagacggca cttcaggcac
    27781 gcgcctgatt gaggcggcaa aacggttttt cggcgaataa accatgctgg ccacgccgcc
    27841 gctcgccccc aaaaccgtcg ccgccctgca caggctcggt atccgtacgc tcgaagaact
    27901 gcggcaaaac ggttcggtca aagcctttct gctgctcaaa gcatcgggtt tgaccctcac
    27961 gaaaagcacg ctgtggcaac tcgagtcgct gctgaacggc acgccgccgc aagaaatgcc
    28021 ccaagcgcat aaagactgcc tgcttgccga gttgaaaaac catccgcccg tcgccgcctt
    28081 tccgccgcag gaagaaatgg gatacttcat gcgcgaagca ctgcgccaag cagaacaatc
    28141 cgccgccgac ggcgaaatac ccgtcggggc agtcatcgtt tcagacggca aaatcatcgc
    28201 atcggcacac aacacctgca ttgccgactg caatgtcagc cgacacgccg aaatcaacgc
    28261 cttggcacag gcaggcagcg aaatgcaaaa ctaccgcctt gacggatgcg acatctacat
    28321 caccctcgaa ccctgcgcca tgtgcgcgtc cgcactgata caggcacgaa tcaggcgcgt
    28381 gatctacggt gcggcagaac ccaaaaccgg cgcggcaggc agcatcgtca acctgtttgc
    28441 cgacaaacgc ctcaatacgc acaccgccat acggggcgga atcctgcaag aagaatgccg
    28501 cgccgtatta agccgttttt tccaaaacaa aagaaaaggt tgaacccatg accgccctac
    28561 tcgtcatcct cgccctcgcc ctgatagccg tcggcacggc aggcatcgtc tatcccgccc
    28621 tgcccggctt ggcattgatg tttgccggaa catggctgct tgcctatgcc ggcggctatc
    28681 aaatctacgg cgcaggcatc ttgtggacgg tcggactcat cagccttggc ggcatactgg
    28741 cggactatat ggcaggcatg ttgggggtaa aatacactgg ggcaggcaaa ctcgccgtcc
    28801 gaggtgcatt ggccggcagc atcatcggca tatttttctc cctgcccggg ctgatactcg
    28861 gcccctttat cggcgcggcg gcaggcgaac tgatcgatcg gcgcaatatg cttcaggcag
    28921 gtaaagcggg cttgggtacg ctgttggggc ttgtcgtcgg cacggcgttc aaaatcggct
    28981 gcgccgtatc catcttgttt atcctgttgg tgaaatacat cgcctacctg ttttaacccc
    29041 ttcagacggc ataccggcaa attgctataa tgccgtctga aatttccgac aaagcaccat
    29101 tatggcaaac caaagaccca tctacggctt ccacgccgtc aacgcccgat tgtggcaaaa
    29161 ccccaaatcg attaccgaac tctatatcca agaaggcaaa tccgacgcac gcacgcgcga
    29221 agtgctggaa aaggcggcaa acgaaaacat ccgcgtatat tttgccgatg ccgaccgcct
    29281 caacgccatc agcaaaggcg cgcgccatca gggcgtggtc ggatttatcg atgcctccaa
    29341 aaaccacgtc cacctcgaag gcatattgga aaacttgagc gaaccgccgc tgctgctgat
    29401 actcgacggc atcaccgatc cgcacaacct cggcgcgtgc ctgcgtaccg ccgacgcaat
    29461 gggcgtacac gccgtcatcg cgccgaaaga caaaagcgcg ggactgaacg ccaccgtcag
    29521 caaagtcgcc tgcggcgcgg cggaaaccgt cccctacatc accgtaacca acctcgcccg
    29581 caccctgcgc gaattgaaag aatacggcat ttggattatc ggcaccgaca tgggcggcga
    29641 cgccgacctt taccattgca atctgcccga cagcacggcg tgggtgatgg gcaacgaagg
    29701 cgacggtatg cgccgcctca cgcgcgaaca ttgcgacatg ctggtgtcca tacccatgtt
    29761 cggcacggtc gaaagcatga acgtctccgt gtccgcagga atggtattaa gcgaaacccg
    29821 ccggcaacgc gtattgaaaa acaaaaaagc gtaagcctca aaaacaatgc cgtctgaaac
    29881 agggattctg tttcagacgg cattcatttt caaaatcagt gttccaaatg gtcggtatca
    29941 aacttgaccg ccgcctcggt tttatcttcc ggcaagacca aattcagcaa gactgccgta
    30001 atgccgccgc cggaaataga gttttggaac aagacgggca ggtttttaaa cacttccggc
    30061 tcaaacccca cgcccaagcc caaaccgacc aacgttgccg caatcaccgc ctcgcgcctg
    30121 cggatgccgt ggccgaccaa aatccgcacg cccgcaatcg caatcaggcc gaacatcaaa
    30181 accatcgcgc cgcccaacac cggactcgga atcgtcgtaa acgcgcgtcc gacaacgggg
    30241 aacagaccca acagcaccaa aatcacggca atatatttgc ccacgtggcg cgaagccact
    30301 ccggtcatct gaatcacgcc gttgttttgc gcaaacgtcg tcagcggcag cgaacccaaa
    30361 gccgtcgcaa tcaccgatac caagccgtca gccaacacgc cgccgcgcag gcgtttggtg
    30421 tattcttcgc cttcaatcgg ctggtcggac accattgccg ttgccgtcaa atcgccgacc
    30481 gcctcaaaca cgcccaacaa gaaaatcgca cccgccgcaa taaatgcgtg ccagtcaaaa
    30541 gcaaaaccat atttaaacgg tacgggcagc gtaaccggcg gcaggttttg cagtgcggaa
    30601 aaatccacct tgcccaaaaa cagcgcgaca atagtagccg gcaatcagac cgaccgcaat
    30661 gccgctcatc cgcagcagcg gatttttcat gcagttgaac accagcacaa tcagcagcac
    30721 cagcgaagcc agccccaagt tttccatcga gccgaacgtg ccgtccgcct tcgcgccgaa
    30781 gccgccgccg aaatcggcaa taccgacgtg taccaaactc agaccaatca gcatcacgac
    30841 cacgccgctg accgtcggcg taatcacttt tttcaaatac ggcaaaagcc acgccgagaa
    30901 acacaccaaa aacgcaccgg caaacgatac gcccaagagc gtcgaaacca tcgccccctc
    30961 actcaaaccg ccctctttca tccccgcgcc gagcgcaatc atgacggtaa cgctggatgg
    31021 acagcatccc cgagccgacc ggcccgaagc ggttgacctg caaataagtg ccgatgccgg
    31081 aagccaccat cgccatcgac acgagatacg ccgtcatttc caccggtagt tccagcgcgc
    31141 cgcccacaat cagcgcgggc gtaatcatcg gcacgaaaat cgccaaaaga tgggtaaccg
    31201 cgctcaagag cgcattaccg aacggcggcc tgtcttccaa accgtacacc aaatcaggcg
    31261 aatccgcctg ttttttcatt gtttcagcca ttggagtctt tctatccgtt gccaaacgtt
    31321 aaaaaatttt aagaacgaaa ttgtaacgaa tcaaagattc cttatcaatt caaatcctca
    31381 tgtgcaaaaa caccaccggt tgtccgaatt caaatttctt tgaatttcca caccggacag
    31441 tccttcattt ccagagagcc ttcatgcgct taacacattt taatcctcaa cggcgcatcc
    31501 actgcttaaa cccaataatt tcatgattat aaaaaaaata ttatttatta agatatgatg
    31561 ggacttaagc cttggaacca accgtgcggc cgatatgttt cacacaaaca acaaagtaag
    31621 ctacaatccg aagcataatc gacctcactc aatgaataaa aggtttccgt atgttacaag
    31681 gtagcttggt tgccctgatt accccgatga atcaagacgg cagcatccat tacgaccaac
    31741 tccgccaatt aatcgactgg cacatcgaaa acggcacgga cggcatcgtc gctgtcggca
    31801 cgacaggcga atccgccacc ctctccgtcg aagaacattt aagcgtaatc gaagaaacgg
    31861 tcaaacacgt tgccaaacgc gtccccgtca tcgccggcac aggggcgaac aacaccgtcg
    31921 aagccatcgc cctttcccaa gccgccgaaa aagccggcgc ggactacacc ctctccgtcg
    31981 tcccctatta caacaaacct tcccaagaag gcatgtaccg ccatttcaaa gccgttgccg
    32041 aagccgccgc gattccgatg atactctaca acgtgcccgg cagaaccgtc gtcagcatga
    32101 acaacgaaac catcctgcgc cttgccgaaa tccccaacat cgtcggcgtt aaagaagcaa
    32161 gcggcaacgt cggcagcaac atcgaactca tcaaccgcgc gcccgaaggc ttcgtcgtcc
    32221 tttccggcga cgaccacacc gccctgccgt tcatgctctg cggcggacac ggcgttatta
    32281 ccgtagccgc caacgccgcc ccgaaactct ttgccgatat gtgccgtgcc gcgctgcaag
    32341 gcgacattgc ccttgcacgc gaactcaacg accggctgat tccgatttac gacaccatgt
    32401 tctgcgaacc cagtcccgcc gcaccgaaat gggcggtatc cgccttgggc agatgcgaac
    32461 cgcacgtccg cctgccgctc gttccgctga cggaaggcgg acaggcaaaa gtccgcgccg
    32521 ccctgaaagc ctcaggacaa ctttaaatat agccatgccg tccgaaccgt tcggacggca
    32581 taacatgaca aacactttaa tatccatcac acaggatgac acgatgaccc atatcaaacc
    32641 cgtcattgcc gcgctcgcac tcatcgggct tgccgcctgc tccggcagca aaaccgaaca
    32701 gcccaagctc gactaccaaa gccggtcgca ccgcctgatc aaactcgaag tcccgcctga
    32761 tttgaacaac cccgaccaag gcaacctcta ccgcctgcct gccggttcgg gagccgtccg
    32821 cgccagcgat ttggaaaaac gccgcacacc cgccgtccaa cagccagccg atgccgaagt
    32881 attgaaaagc gtcaaaggcg tccgcctcga gcgcgacggc agccaacgct ggcttgtcgt
    32941 tgacggcaaa tcccccgccg aaatctggcc gcttctgaaa gccttttggc aggaaaacgg
    33001 cttcgacatc gaatccgaag aacccgccat cggacaaatg gaaaccgagt gggcggaaaa
    33061 ccgtgccaaa atcccccaag acagcttgcg ccgcctattc gacacagtcg gtttgggcgg
    33121 catctactcc accggcgagc gcgacaaatt catcgtccgt atcgaacagg gcaaaaacgg
    33181 cgtttccgac atcttcttcg cccacaaagc gatgaaagaa gtgtatggcg acaaaaacaa
    33241 agacacgacc atgtggcagc cttccgcttc cgaccccaac cttgaggccg ctttcctgac
    33301 gcgctttatg caatatttgg gcgttgacgg acggcaggcg gaaaacgcat tggcaaaaaa
    33361 accgaccctt cccgccgcca acgaaatggc gcgtatcgaa ggcaaaagcc tgattgtctt
    33421 tggcgactac ggcagaaact ggcggcgcac cggccttgcc ctcgaccgca tcggactgac
    33481 cgtcgtcggt caaaacaccg aacgccgcgc cttcctggtt caaaaagccc cgaacgaaag
    33541 caatgcagtt accgaacaaa aaccggggct gttcaaacgc ctactgggca aaggcaaagc
    33601 ggagaaacct gccgaacagc cggaactgat tgtctatgcc gagcctgtcg ccgacggttc
    33661 gcgcatcgtc ctgctcaaca aagacggcag cgcatatgcc ggcaaagacg catccgcact
    33721 gttaggcaaa ctccattccg aactgcgtta aaccgttcaa aacaaaagca gcttccaaac
    33781 agaagctgct tttttgtgcg gaacttttta caacaaacgg ggcaaaatat cctcaaccgc
    33841 ctgaaccaac gcatccgcca aaggcggatc gaacgcacaa tgccccgcct ggaccaccct
    33901 caattccgct tcgggaaagg ctttcgacag ctcccacgca ctctgcatag gcgtacacaa
    33961 atcataccgc ccctgcacga taacggtcgg gatatgccgt attttgccga tattgttcaa
    34021 aatcgcctta tcaccctgca accaaccgcc gttgacaaaa taatggtttt ccaaacgcgc
    34081 gattgccagt gaggcataag catcttcatc cactccctcc ggctcgaaac gaatcagata
    34141 gctttcccaa tccgcccaag ccttcgcggc agacaggcac acttcttcat cttgatgaaa
    34201 cagcaacccg tgatacgcct caatcagccg gttccgccga ttttcagcaa tcggcgcgac
    34261 aaatttttgc cattgttccg gataaatccg gctcacgccg cccgcctcgt tcagccacgc
    34321 cgtttcagac ggcctgcaca aaaatatccc gcgcaacacc aatcccttta cccgttcggg
    34381 atgggtttgg gcataagcca gcgacaaagt gctgccccac gaaccgccga acaccagcca
    34441 tttcccgata cccagcattt cacggacttt ttcaatatcc gccaccaaat cccaagtcgt
    34501 attgtcttcc gcacaagcat acgggtgcga acgtccgcaa ccgcgctggt cgatgatgac
    34561 gatgcggaac acatcaggat tgaaaaaacc ccgacattca ggcgaagccc ccgcgcccgg
    34621 cccgccgtgt aaaaaaatga ccggcacacc gtcgggattg cccgactctt cccaataaat
    34681 ttgatgaatt tcagacacct gcaagtatcc gctatgaaaa ggctgtttta tttcatacat
    34741 catcttctcc catattgctt ctgacaatcc gaacatcccg acacgcgaca tcccgcacga
    34801 aacacaagcc gccccgaaag gcaaatgccg aaaaatcgcg ataaaaacca tcaatcggaa
    34861 aatattgcat aaacaggcga aaatatatca ttgaaaagaa aacccataca atgtctgaca
    34921 gaaacaagcc gctttgccat tgtttcctaa cagttaaacc ccgcccttca aggcggcggg
    34981 gcagggcttg attcaaaatg gcgcaagccc ctgccctcaa atccaacacg caggattaaa
    35041 ccataatagc ggctttctta ttatttctta ttgaaacacc gcccggaacc cgatataatc
    35101 cgcctttgaa gcatcagtga aaatcttttt tttaaccggt taaaccgaat aaggagccga
    35161 aaatgaatcc agcccgcaaa aaaccttctc ttctcttctc ttctcttccg ccgcgcaggc
    35221 ggcaagtgaa gggaatggcc gcggtccgta tgtgcaggcg gatttagcct acgccgccga
    35281 acgcattacc cacgattatc cggaacaaac cgctccaaaa aaagcacaat taagcacggt
    35341 aagcgattat ttcagaaaca tccgtacgca ttccatccac cccagggtgt cggtcggcta
    35401 cgacttcggc ggctggagga tagcggcaga ttatgcccgt tacagaaagt ggaacaacag
    35461 taaatattcc gtcagcataa aagagttggg aagaaacgat aatagcactt ctaacagcag
    35521 ccatcttaac ataaaaaccc aaaagacgga acatcaagaa aacggcacat tccacgccac
    35581 ttcttctctc ggcttatcag ccatttacga tttcaaactc aacgataaat tcaaacccta
    35641 tatcggcgtg cgcgtcgcct acggacacgt taaacatcag gttcgttcag tggaaagcga
    35701 aaccacgact gttaccactc acaatggagc ccctgtccca caaggtccga cccccaaacc
    35761 tgcctatcac aaaagccgca gcatcagcag cttgggcttc ggggcagtgg caggcgtagg
    35821 catcgacatc acgcccaagc tgaccctgga cgccggctac cgctaccaca actggggacg
    35881 cttggaaaac acccgcttca aaacccacga agcctcgttg ggcgtgcgct accgcttctg
    35941 attcgccgat tccgatgccg tctgaacctt cagacggcat ttttgacgcg cccgccgttt
    36001 acgggcgcgg ggcaggtgcg gggaaatacc cgtaccgcca ttgccgatac accgtaaccc
    36061 taaaacccgc cattcccgac aacaccgtaa tctcgaaacc cgtcattccc gcgcaggcgg
    36121 gaatccggac ccccgacgcg gcgggaatct atcggaaatg actgaaaccc cgcgtcctag
    36181 attcccactt ccgtgggaat gacggttcgg ttgctacggc ccgctgattc cccgacaccg
    36241 atgccgtctg aaccttcaga cggcattttt gatgcgcccg ccgtttacag gcgcggggcg
    36301 ggcgcagtaa aatacccgaa ccgtcattcc cgacaacacc gcaatcgcga aacccgtcat
    36361 tcccgcgcag gcggaaatcc ggacctgtcc gcacggaaac ttatcggata aaacggttgc
    36421 ccaaaccccg cgtcctagat tcccacttcc gtgggaatga cggttcggtt gcgtagggcc
    36481 gggtggtcga aagggcggat tcgatggatt cgatggattc gatgaaaacg gtagacatgt
    36541 tggattgatt gtttattgtt gaggaaaata taagatcttc tttccggttg aagccgattt
    36601 gtttttatct tatgggtgtt atgctatatc atcatagtta tgcagaagat gcagggcgcg
    36661 cgggcagcga ggcgcagata caggttttgg aagacgtgca cgtcaaggcg aagcgcgtac
    36721 cgaaagacaa aaaagtgttt accgatgcgc gtgccgtatc gacccgtcag gatatattca
    36781 aatccggcga aaacctcgac aacatcgtac gtagcatacc cggtgcgttt acacagcaag
    36841 ataaaagctc gggcattgtg tctttgaata ttcgcggcga cagcgggttc gggcgggtca
    36901 atacgatggt ggacggcatc acgcagacct tttattcgac ttctaccgat gcgggcaggg
    36961 caggcggttc atctcaattc ggtgcatctg tcgacagcaa ttttattgcc ggactggatg
    37021 tcgtcaaagg cagcttcagc ggctcggcag gcatcaacag ccttgccggt tcggcgaatc
    37081 tgcggacttt aggcgtggat gacgtcgttc agggcaataa tacctacggc ctgctgctaa
    37141 aaggtctgac cggcaccaat tcaaccaaag gcaatgcgat ggcggcgata ggtgcgcgca
    37201 aatggctgga aagcggagca tctgtcggtg tgctttacgg gcacagcagg cgcggcgtgg
    37261 cgcaaaatta ccgcgtgggc ggcggcgggc agcacatcgg aaattttggt gaagaatatc
    37321 tggaacggcg caaacagcaa tattttgtac aagagggtgg tttgaaattc aatgccggca
    37381 gcggaaaatg ggaacgggat ttgcaaaggc aatactggaa aacaaagtgg tataaaaaat
    37441 acgaagaccc ccaagaactg caaaaataca tcgaaggtca tgacaaaagc tggcgggaaa
    37501 acctggcgcc gcaatacgac atcaccccca tcgatccgtc cggcctgaag cagcagtcgg
    37561 caggtaatct gtttaaattg gaatacgacg gcgtattcaa taaatacacg gcgcaatttc
    37621 gcgatttaaa caccaaaatc ggcagccgca aaatcatcaa ccgcaattat caattcaatt
    37681 acggtttgtc tttgaacccg tataccaacc tcaatctgac cgcagcctac aattcgggca
    37741 ggcagaaata tccgaaaggg tcgaagttta caggctgggg gcttttaaag gattttgaaa
    37801 cctacaacaa cgcaaaaatc ctcgacctca acaacaccgc caccttccgg ctgccccgcg
    37861 aaaccgagtt gcaaaccact ttgggcttca attatttcca caacgaatac ggcaaaaacc
    37921 gctttcctga agaattgggg ctgtttttcg acggtccgga tcaggacaac gggctttatt
    37981 cctatttggg gcggtttaag ggcgataaag ggctgctgcc ccaaaaatca accatcgtcc
    38041 aaccggccgg cagtcaatat ttcaacacgt tctacttcga tgccgcgctc aaaaaagaca
    38101 tttaccgctt aaactacagc accaatgcaa tcaactaccg tttcggcggc gaatatacgg
    38161 gctattacgg ctcggaaaac gaatttaagc gggcattcgg agaaaactcg ccggcataca
    38221 aggaacattg cgacccgagc tgcgggcttt atgaacccgt attgaaaaaa tacggcaaaa
    38281 agcgcgccaa caaccattcg gtcagcatta gtgcggactt cggcgattat ttcatgccgt
    38341 tcgccggcta ttcgcgcaca caccgtatgc ccaacatcca agaaatgtat ttttcccaaa
    38401 tcggcgactc cggcgttcac accgccttaa aaccagagcg cgcaaacact tggcaatttg
    38461 gcttcaatac ctataaaaaa ggattgttaa aacaagatga tatattagga ttgaaactgg
    38521 tcggctaccg cagccgcatt gacaactaca tccacaacgt ttacgggaaa tggtgggatt
    38581 tgaacgggga tattccgagc tgggtcggca gcaccgggct tgcctacacc atccgacacc
    38641 gcaatttcaa agacaaagtg cacaaacacg gttttgagct ggagctgaat tacgattatg
    38701 ggcgtttttt caccaacctt tcttacgcct atcaaaaaag cacgcaaccg accaatttca
    38761 gcgatgcgag cgaatcgccc aacaatgcct ccaaagaaga ccaactcaaa caaggttatg
    38821 ggctgagcag ggtttccgcc ctgccgcgag attacggacg tttggaagtc ggtacgcgct
    38881 ggttgggcaa caaactgact ttgggcggcg cgatgcgcta tttcggcaag agcatccgcg
    38941 cgacggctga agaacgctat atcgacggca ccaacggggg aaataccagc aatgtccggc
    39001 aactgggcaa gcgttccatc aaacaaaccg aaacccttgc ccgacagcct ttgatttttg
    39061 atttttacgc cgcttacgag ccgaagaaaa accttatttt ccgcgccgaa gtcaaaaacc
    39121 tgttcgacag gcgttatatc gatccgctcg atgcgggcaa tgatgcggca acgcagcgtt
    39181 attacagctc gttcgacccg aaagacaagg acgaagacgt aacgtgtaat gctgataaaa
    39241 cgttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt attgaccaat ttcgcacgcg
    39301 gacgcacctt cttgatgacg atgagctaca agttttaaag gcagcccgcg ttttgtggaa
    39361 aaactgcaat gccgtctgaa agcccttcag acggcatttg tttccccaaa cgcatcatcc
    39421 tgccgcaagc ctatgccaat ccgttttatc gcatcggcaa ctcaaagaaa aatccatttc
    39481 attcccacgc agggaagccg gtttttgatt tcggttattt ttggttgttt cgggtaattt
    39541 atgagtcgtc attcccgcaa aagcgggaat cagttttttt atagtggatt aacaaaaaca
    39601 ggtacggcgt tgcctcgcct tgccgtacta tttgtactgt ctgcagcttc gtcgccttgt
    39661 cctgattttt gttaatccac tataaatttc agccatttcc gataaattcc tgtggcttta
    39721 gctttccgga ttcccacttt cgtgagaatg gcggcggaga ggtttctgtt tttccgataa
    39781 atcccacaac ctaaaatctc atcattaccg cgcagatgat atgttgcccg tcaacaccaa
    39841 ataaaaataa aaccgtaata aattgattta tgttgttatt t
//
LOCUS       DAXFWM010000011        39039 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000011 DAXFWM010000000
VERSION     DAXFWM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39039
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.011"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(126..1043)
                     /locus_tag="ACFI8B_000542"
     CDS             complement(126..1043)
                     /locus_tag="ACFI8B_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC3833831.1"
                     /translation="MNNSNLGLENIGNLSELLGNAEVSSGTPLRLPLASIKEDPDQPR
                     KEFDEGKIQELANSIQERGVKTPISVRQSSEEGVYVINHGARRYRASLLAGLSEIPAY
                     IDQDYSLIDQAVENIQRDNLTPREIADVIGRLLAEGMKKGQIAEKMGKSPAFVTQHAN
                     LLDLPEPLANAFNSGKVSDLTVINDLNALYKKEPEAVEDLLSNEDEITRSSVRNLKEF
                     LNSDPKNSADADHEKPSKAKVKEKKPEDPDKLKKAIVQVMYQGQLARLVLNKRGLSGD
                     EVWIKFEESGETESVPCKEISGIVAVIEG"
     gene            complement(1049..1804)
                     /locus_tag="ACFI8B_000543"
     CDS             complement(1049..1804)
                     /locus_tag="ACFI8B_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3833832.1"
                     /translation="MKILVVANQKGGVGKTAMSLHLAWHMETVGLRVLVIDLDTQGNA
                     SYSLRDKTCLFGSGRLFGNMEDADLHMPGNVSLALSPATNDLANVQNMTLQNAVQSFT
                     QNIDKLKAGGQFDVCIIDTPPSLGNTLAAALAAGDYVLCPIELETYSLQGIKQMAATI
                     GNIRKVNSKLAFLGILPSKVDMRNPRHKRHLEEIKMQYNEIVIPHIIGLRSSIADALS
                     SSLPVWKIKKTAARKAAQEMKAVSNYVLNKMQG"
     gene            complement(1814..2059)
                     /locus_tag="ACFI8B_000544"
     CDS             complement(1814..2059)
                     /locus_tag="ACFI8B_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833833.1"
                     /translation="MDRIKSKAGKKMFEVVYMVLAIAYLLFPVRWFAWMYSFYVLCQM
                     LYHWNMADSWIYIGKFILTFGIVMGWLTFVIAFRNKI"
     gene            complement(2140..2487)
                     /locus_tag="ACFI8B_000545"
     CDS             complement(2140..2487)
                     /locus_tag="ACFI8B_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833834.1"
                     /translation="MTDLISTTVLLTPYQAFQAKKWHLQNIEEHNLIQKKDLNIRPEC
                     FHRFPQWAVPTADEVKELWLATGMTQKQVSRYLGLKDTNGRAFRRFISGESSITYAYW
                     VLLSLYVGIEKFW"
     gene            complement(3105..3983)
                     /gene="trfA"
                     /locus_tag="ACFI8B_000546"
     CDS             complement(3105..3983)
                     /gene="trfA"
                     /locus_tag="ACFI8B_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HFC3833835.1"
                     /translation="MKNVQEVLPYLNLVAERRDEAQREEYLKSKGIPTDDNLIIEDLF
                     GNTIFGSKHRAMPNDFARSCLFTARNHRVPRKTFSREKLFHISEKVEILYTGTELRSV
                     DDELIWMQLVDYCRRFPLGNYVEFDIRQLIKDIGWDTSGAYYKRVRESLSRMKATEIY
                     IKNGNTYGVSGGISLIDNYVGIENIKGEPTRYKVSIDKKLIVLFAGNTFSNIPWSQYK
                     KLSPMARRLTDYVFSHKHPNPIPIPAFLAMCGSDQINSPAKTQNQNAKKICAELTEAQ
                     LVKTAFVLSGKIIVER"
     gene            complement(3987..4370)
                     /locus_tag="ACFI8B_000547"
     CDS             complement(3987..4370)
                     /locus_tag="ACFI8B_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3833836.1"
                     /translation="MNKFIISGRTTKDLTLEQTATGVFTTSIPLAENYFYTKDGKKVE
                     ATNYFWIDVYGEQAKNAVRYCGKGSLILIEGRISNNNYEKDGQKIYKDQMIASRIEYS
                     NLKPPAGREESSDPSFIYDPEQDLG"
     gene            4430..4795
                     /locus_tag="ACFI8B_000548"
     CDS             4430..4795
                     /locus_tag="ACFI8B_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3833837.1"
                     /translation="MSYSKIFFTNVLRLASEKNLSHQDLAERANLSPSSMSGITRGQG
                     NPTLETMSAIAYALDVPLSYLLEHHDLDPESLSLLDGSQSTLPPNYEKITVILPRYKA
                     FQVKKWNDDALKILAKETK"
     gene            4924..5922
                     /gene="trbB"
                     /locus_tag="ACFI8B_000549"
     CDS             4924..5922
                     /gene="trbB"
                     /locus_tag="ACFI8B_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HFC3833838.1"
                     /translation="MMETTETADSRKERALNKLKRDLGNEFLEKFMNPNLVEVMFNPK
                     QTGNGVSVYWEEQGKEMVYDERLSDWFKTSRVEAVIKTVAGYHGKVITEDLPILESQF
                     PIDGSRFTAILPPVTASPSFTIRKKPARVFTLDEYVSAGIMTESQRQTIRNAVSEHGN
                     ILVIGGTGSGKTTLINAILYDMSEQNPNERLYISEDTGEIQCHSQNKVQVYTTGSIDL
                     TKLVKTALRYRPDRIIVGEVRGAEALDLLDIWNTGHPGGIATLHANNAALGLERLESL
                     ITRNPAHPAAIRPLVAQVIHYIVSIGKDEKTGRKIREIIKIKGFDEKSGNYVFDYL"
     gene            6024..6407
                     /gene="trbC"
                     /locus_tag="ACFI8B_000550"
     CDS             6024..6407
                     /gene="trbC"
                     /locus_tag="ACFI8B_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer system pilin TrbC"
                     /protein_id="HFC3833839.1"
                     /translation="MTLSQSQQKWLFFIGLAVFLLLMSSPALASQGQGGGLPYESWLN
                     NLRQSVTGPVAFALSIIGIVVAGGVLIFGGELNAFFRTLIFLVLVMALLVGAQNMMSS
                     FFGGAEVTAAVQAAQHYLLPLASKG"
     gene            6412..6729
                     /gene="trbD"
                     /locus_tag="ACFI8B_000551"
     CDS             6412..6729
                     /gene="trbD"
                     /locus_tag="ACFI8B_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFC3833840.1"
                     /translation="MRAIRTIPIRRVANRPNLFWGGDREMVQVSLLITFVLVVAAQNT
                     YAFVIGLVFWFFALYALRKLAKYDPLLRFVGLRSITKYKRFYPPHSTPFRNNTSTQGN
                     RYK"
     gene            6726..9290
                     /locus_tag="ACFI8B_000552"
     CDS             6726..9290
                     /locus_tag="ACFI8B_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HFC3833841.1"
                     /translation="MIIGIISAISVLGLVLCTGLFLKIRAVTNATKLKQHRSKEEGLA
                     DLLNYAAVIDDGVILGKNGSLMAAWIYRGKDMGSSTVAEREDVSLRINQAFVGLGSGW
                     MVHVDAARRPAPNYTEAGRNYFDSYVTEAIDNERRQAFEAIGTLYEGFFVIVATYFPP
                     LLAEKKVAELMFDDDNAKEGKRAQQDRTLSHFKQACANLESNLSISLELHRLCAHKVV
                     LEDGMEITQDDFLRWLHFCITGKNHPINLPAAPMYIDSLIGGQELYTGVVPMIGDKFI
                     QVVALDGFPMESYPGILTRLGEMPIEYRWSNRFIFMDQHQAVADLEKYRKKWKQKVRG
                     FFDQVFNTHSGSIDQDAQAMVDDAEAALAETKSGLVAQGYYTSVVILMHEDRAFLESM
                     ARQVEKEINTLGFNARVETVNTMEAWLGSLPGHGIENVRRPLLNTLNFADMIPTSSIW
                     TGLEENPCQFYPPNSPPLMQVVTSGSSPFRLGLHVGDVGHTLVFGPTGAGKSTLLATL
                     AAQADRYRNMRVFSFDKGRSLETLTRAVGGSHYNIGDEDNTASGLSFAPMQLIQNTSD
                     RAWALSWVEAVMELNGLKVTPQQRNEIETAIKNNAAEQSYSLSDLSNTLQDVEMREVL
                     KAYTIDGSMGYLLDAEQDSLSFSRFTCFEIEELMNLPEKYALPIMLYLFRRIEKALTG
                     DPAVLFLDEAWIMLGHPVFREKIREWLKVLRKKNCLVLLATQSLSDAANSGILDVLNE
                     SCPTKIYLPNAHAREEDASELYRRMGLNARQIEILATAIPKRQYYYTSPYGRRLFELA
                     LDPLTLAFVAVSDPDTLNAMRQTADQYGEDWPIRWLQNRGINIHNYVDVDKYRRKH"
     gene            9290..9997
                     /locus_tag="ACFI8B_000553"
     CDS             9290..9997
                     /locus_tag="ACFI8B_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFC3833842.1"
                     /translation="MGLKDIFRVREKADREMTENPYLNARKQWNFIMGDMVSSRLLWQ
                     WAGMTGLLIGLGGVGGMIYLGSQSKFIPYIVEVNKLGETVAVARADQPQATDERIVRA
                     MLGSFIGNARTVTTDIALQRKYVLNTYAMLNPKDAAAAKMNEWLNGTPESSPFKRAEK
                     ELVSVEIKSVLRQSDNSWQVDWVETTRAHDGKLLKPPQNMRALLNIYYVPPTTEQQII
                     VNPIGMFIRDFSWSNEL"
     gene            10010..10876
                     /gene="trbG"
                     /locus_tag="ACFI8B_000554"
     CDS             10010..10876
                     /gene="trbG"
                     /locus_tag="ACFI8B_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HFC3833843.1"
                     /translation="MKRQILSLALITALSTSAFADSYADKYFEGSHNPKLNRHEQKGL
                     GIAKNYSSDGRMAVPPVPGDNGRITFAFGSDPSVVCAVMQVCDIALQPGEMINDLHAG
                     DTARWNITPAISGSNGNERMHILVKPLDVGLQTTLFIATDRRTYHIRLKSHRTRYMPA
                     VGFSYPEDTAARWRAIRANQARQAEAQVLPTGENIQNLDFNYTISGHNPSWKPLRVYN
                     DGTRTIIQMPPTLSQGEAPALLVVRNGENTLVNYRLHEDRYIVDSLFDEAIMITGVGR
                     HQTKVVIRRSRS"
     gene            10873..11283
                     /locus_tag="ACFI8B_000555"
     CDS             10873..11283
                     /locus_tag="ACFI8B_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HFC3833844.1"
                     /translation="MKSNVLFVALALAVGGCATGQYGSFVRSDAVSASVTQDVVEKLQ
                     EEFPAANTVFFLNRSKSDTFGTQLDQALREKGFATTAQQGENEKSLNYVLDQVDSDIY
                     RVMIYIDGTPYSRAYRQTENGAVVPTGSWTKRGD"
     gene            11287..12726
                     /locus_tag="ACFI8B_000556"
     CDS             11287..12726
                     /locus_tag="ACFI8B_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC3833845.1"
                     /translation="MSQSTEDLLNASRSPSIAPDTSDRRPGVRRLNNIPIYIVGGVVL
                     GFALLIALVAATKGQQSAGQATQGDGKDATRMADSLLNELPQEGGLITAKASEPAMPA
                     LPDGMASAPDLPASAPTSLPMVPDMANLPATPPEVDQEAEQIRQAKAQMFQAALAAPT
                     KVDVVASTSRGSSGLYTAALSSSGSGTPNRSEMLAQIAEVQRMSENAGSNPAEVERVY
                     NQQLANAQQMAAAGGQDGVQGAGGMPGLNTPPTPGNIPRQTAQNGGNNWRLESQVERP
                     NAYMIRSGAVIPATLISGINSDLPGMVQAQISQNVYDTATGRHLLLPQGSRLVGTYSS
                     GINLGQRRLFIAWNRIIFPDGKALDIGDMPGASGAGYAGFSDRINNHYGRLWGNALML
                     SLVGASITYGVDKNSNNNNNGNNNGNNSTVRGALSESLGQTFGQAVSQSIQKNMNVSP
                     TLEIRPGYRFNVILTKDIDFSKPYRSFDY"
     gene            12739..13515
                     /gene="trbJ"
                     /locus_tag="ACFI8B_000557"
     CDS             12739..13515
                     /gene="trbJ"
                     /locus_tag="ACFI8B_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFC3833846.1"
                     /translation="MQKRLLSKTKTVALSLAVSVSLGLSAPAYAGIPVTDGGNIAQSS
                     VSAIQNVAQTLKQIEEYRIQLKQYETQLRNSLAPAAYVWDQANKTMANMKAAVARLRA
                     YKQQLGNMEEYLSKFQAVDFYKSSPCFTAKGCSAADRAKLEQNYAQLSRMQKLSNDGL
                     LRSINNEIDSISRDANTLTQLQRNAQSATGQLEALQYANQFHSAQAHQLLQLRNMINT
                     EMQANAAYRQSQLDREARYEAARKQVRTSDILKHPYRRGQ"
     gene            13750..15357
                     /gene="trbL"
                     /locus_tag="ACFI8B_000558"
     CDS             13750..15357
                     /gene="trbL"
                     /locus_tag="ACFI8B_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HFC3833847.1"
                     /translation="MKKKYIYYFIFFITLSMPIISHAAGQGGVLDEALSRYEGKIGAW
                     EAAFYKAGLFIFWSLSSISIVMTGAQLIFQRDNISSFFAEFTRLILFLGFFLWIITNG
                     IKITTSIKDGLMKLAGQASRNGEQITPSSIVDIGFNLFDKIVENSSIWDPIDSATLIV
                     IGLVILLILAAVGINMLLMLITTYIVIYSGIIILGFGGGRWTSDMAVGYLKQVLNLSL
                     QLAAMILIIGIGQSIVQDTINTIGTPGFRELAVVLVQTALLVGLVVKIPPMIGSLAGG
                     AGSGGIGSFGVGNAIAAAAMLAGAAAGAGAALKSLAVEAGGLKKAFEAAKAGLGGSNG
                     SSDSGSGKMDSAVDAVAGANAGDKSNQDGKPLTAESSGNSENGSEGSTTGNSGGNPSG
                     SSEENTISSGSSSSSGEANSTNTSASSGAEGSAEGEATSSETSQSASDSPSNNSGEAT
                     QTADTTPKTTNTGGGSPLATAMGGNKVSNWQAAKNVAVGLVSSKWNNAVNNSAGGRLV
                     KHWEDEKKKTEDPTKNPQKNPEGDDNA"
     gene            15350..15922
                     /locus_tag="ACFI8B_000559"
     CDS             15350..15922
                     /locus_tag="ACFI8B_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFC3833848.1"
                     /translation="MLKKTLICILLVGSVHFAMAESLNDALNRSRSGNSNNNRPQVED
                     LFTGDVKLACEATLCLSTGNRPSECQPAIHRYFSIRHKRMGDTIRARHNFLRLCPASK
                     EQGMLELVNAIANGAGRCDARELNRINRSTYIKRVWVGSRRHGDWQEVKVPYIRNAKP
                     SYCSAYFENGWTQLGTRYVGEEKNGGKWVD"
     gene            15922..16521
                     /locus_tag="ACFI8B_000560"
     CDS             15922..16521
                     /locus_tag="ACFI8B_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HFC3833849.1"
                     /translation="MVYVADLPPFMQERVTCSIAAAEKYHLPPHYLLAVAERENGRPG
                     QQVRNTNGTYDVGAMQINTAYIRTLSRYGVTQSAVAQPGCYAYYLAAWRIKRHIVYDR
                     KGDIWTKIANYHSYTPKYNAIYRAAIIRLAEKWERWLAGRQGNNAKGNVPTQAVPAVR
                     TIRSQPLPKQYVSPTTVYVPRTIRVVERSPEPEPETVQN"
     gene            16630..16914
                     /locus_tag="ACFI8B_000561"
     CDS             16630..16914
                     /locus_tag="ACFI8B_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFC3833850.1"
                     /translation="MAYAIAFDLDTTVLQELYPNSSWQNAYGDVKRTLVGLGFNHQQG
                     SVYFGNSEITAVSCVLAAQKLSQTYAWFKPAVSDIRMLRIEEMNDLQPAL"
     gene            complement(17024..17578)
                     /locus_tag="ACFI8B_000562"
     CDS             complement(17024..17578)
                     /locus_tag="ACFI8B_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC3833851.1"
                     /translation="MIIGYLRVSTKEQNVAAQRQVIQDKYQIERWYEDQAASGAVKAL
                     NRPEFRKMAEFVRQGDTVVVVAIDRLGRNTIDVLETVQFLQKKGVAVTSLRENFDLAS
                     PLGTAMLAIVSAFAELERSYIKERQMAGIRNAREEGKKLGRKVKVDPEEIRRWRQSEQ
                     TSISRTAAHFNVSESTVKRACKAK"
     gene            complement(17630..18061)
                     /locus_tag="ACFI8B_000563"
     CDS             complement(17630..18061)
                     /locus_tag="ACFI8B_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC3833852.1"
                     /translation="MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLA
                     DILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVRLSPFLNGCPHDLSLGAESLGKG
                     ESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ"
     gene            complement(18076..19281)
                     /locus_tag="ACFI8B_000564"
     CDS             complement(18076..19281)
                     /locus_tag="ACFI8B_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC3833853.1"
                     /translation="MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQH
                     ADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHNSAAVLSSTQFDEVLKRDEPQKK
                     EVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
                     VSMRAERASDNTYKRFNEYGRGASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQD
                     RNSEFIDKFDDVIKMLSLGSQEQILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESV
                     FAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGIYLLGQAKKAETGQT
                     YSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLE
                     YSAKKSISR"
     gene            complement(19288..19473)
                     /locus_tag="ACFI8B_000565"
     CDS             complement(19288..19473)
                     /locus_tag="ACFI8B_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833854.1"
                     /translation="MNKVEPQESNAIKMIKEACEKNRRMMTDEAFRKEVEKRLYAGPS
                     PELLAKLRVLWAANKEQ"
     gene            complement(19507..20715)
                     /locus_tag="ACFI8B_000566"
     CDS             complement(19507..20715)
                     /locus_tag="ACFI8B_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC3833855.1"
                     /translation="MSDYQKLSDAGRAEIVAEYMSALLEITQAVDVPQIALVAAQPGA
                     GKSKAADIVKEEFASKGGHIHVDADIMRQKIPVPPGVVYSSQQTQEDAGKLAVGVRKS
                     ALENSRNVLEEGTFRNAEAVGMSIKAAREAGLKIEMLAVATAPEESLAGIFKRYEDQY
                     LTKNIQPRFVDEDFHNKAFEGFKNTVATHEAEFDRIRVTNRPGEILYDSLNKQQNKQA
                     SAKDAMEFYQQITPERLKQVAQVWDVIQLQADRRSQDPVPNYFDKVKQHREEIYQRVE
                     EIYRQERVVANSEGATLQRKSGDTWQDIEKAEAKGMKAGIHMLGTAKPAESGKEYSGE
                     IVHKDEASVFQKTDQGLIRHKAVQGMSEGKFSSLSEQVEIGQKVSIKREGNGLSVKAS
                     DASVKKTMKR"
     gene            complement(20712..20960)
                     /locus_tag="ACFI8B_000567"
     CDS             complement(20712..20960)
                     /locus_tag="ACFI8B_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833856.1"
                     /translation="MKEVIPGINYEVALEVLGNAAQPFIQAREEERKKSHPNEAFIQY
                     CSDSIKAITVFRHNLRATDEDVIARILDPNDTIVGGSV"
     gene            21445..21918
                     /locus_tag="ACFI8B_000568"
     CDS             21445..21918
                     /locus_tag="ACFI8B_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833857.1"
                     /translation="MPHEQMIDAFSAIIQNSLSLYEEQTPLYYYLTICGSWVLTALGI
                     LSLASVFFTIKQKKPYRFIVPILFLWLGYESIHMPRPNLAVFYGYNLAQLSYPANENP
                     NSFLHLADGSNPICCFIGEDEYLQAKRLGFKEYADAKAKALVDLVEMEKKSKQSD"
     gene            complement(22070..22966)
                     /locus_tag="ACFI8B_000569"
     CDS             complement(22070..22966)
                     /locus_tag="ACFI8B_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine modification methylase"
                     /protein_id="HFC3833858.1"
                     /translation="MFTHSVCSYPQRGNYGKSGYRGNCTGHIIRDFVESYLAPEGLFA
                     DPSIGSGTSSDVAEEMGIRFKGTDLHQGYNLLTQDFAAFLGEQANLIWWHPPYWNMIR
                     YSGNQWGDAANPWDLSQMDLEQFQEAMLLAMMNIHDATEQGGHYGILMGNMRKDGRYY
                     NLSSMVERMAAGRLVDEIIKIQHNCVSDSRQYGGKLVRIAHEKLLVFKKEIQTALYFL
                     AKAMNRVEAVTAVTWKAAVRRVLQAADGRNMGLTEIYGEMEPYAAARAENRNWQAKVR
                     QVLQDERFFLRVGKGIYALKTE"
     gene            complement(22970..23311)
                     /locus_tag="ACFI8B_000570"
     CDS             complement(22970..23311)
                     /locus_tag="ACFI8B_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833859.1"
                     /translation="MLYWDDVSDSFDGWMNLLRQLGGDSFINFEQDIWETARTYETVP
                     HFGNLRQHHLLERLQDTIRNRWPFLRTGFLINALDTHFYVNDEPVETMRDLDAVLGCH
                     YRENEDDLKEE"
     gene            complement(23325..23639)
                     /locus_tag="ACFI8B_000571"
     CDS             complement(23325..23639)
                     /locus_tag="ACFI8B_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833860.1"
                     /translation="MASYTNRLTGHPNVFVEQNIWSNGELMGFSPINVMWDGRNAPTL
                     LCRYTFDGGQYYSLQVSEAAELETRGYQIVCDDLQCLKLKTAKARRSGILALILADAG
                     IE"
     gene            complement(24006..24365)
                     /locus_tag="ACFI8B_000572"
     CDS             complement(24006..24365)
                     /locus_tag="ACFI8B_000572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833861.1"
                     /translation="MSLSAENRTFYLVETGEIGFGSSALFISQSLEEAEAVVADFFKG
                     DEKLHYETDGNQPEISLGMGEPQEEEYIPFLPREPFFWTKGGFGMMGTEYVTIRKTIN
                     GYLEGAGSFRLIKSQQD"
     gene            complement(24362..24796)
                     /locus_tag="ACFI8B_000573"
     CDS             complement(24362..24796)
                     /locus_tag="ACFI8B_000573"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC3833862.1"
                     /translation="MSIYRPASRERKNIKFFADGQYIQEGYKALCWMMRDVVDNHQMH
                     AININLINLLFAQQQYLRDLGRPNPELVLHSGFRTRRHNDSLEGAAKNSQHLSGNAGD
                     FHIERASLSELAALARRFRVGGIGIYPTFIHNDIGVYREWRK"
     gene            complement(24869..24922)
                     /locus_tag="ACFI8B_000574"
                     /pseudo
     CDS             complement(24869..24922)
                     /locus_tag="ACFI8B_000574"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
     gene            complement(24919..25413)
                     /locus_tag="ACFI8B_000575"
     CDS             complement(24919..25413)
                     /locus_tag="ACFI8B_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HFC3833863.1"
                     /translation="MDKDKLYRVAFFLTYHKQAREGWFWRVFGMAFDGRLRQTLQNIA
                     VDYRKTSQNQPLAFDDYLEMLQRHEEEKMGVSAERNQSEQAAVKAFQKQYGLNHRVDT
                     HADTKTLSITITATDEDFIPAVEMGLEQVLGSLAAGETANEGGNEYGRYAFMVSGNVK
                     GRER"
     gene            complement(25422..28421)
                     /locus_tag="ACFI8B_000576"
     CDS             complement(25422..28421)
                     /locus_tag="ACFI8B_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFC3833864.1"
                     /translation="MQNKKPFYEQVAEKLIEQLRQGTAPWQKPWDGGGLPDFMPHNAI
                     TGRRYRGINNLWLQAQGRKDPRWITYKQASSIGAQVRRGEKGTVVEYWQFTEEVPKTD
                     EKGQEIIGQDGKPEKETVRLERPKVFHAVVFNGEQIDGLPPLKLRPLSWNPDERAENI
                     LKNSGAVIDHRHGDRAFYSPSRDSITLPLREQFESPGAYYETALHELGHWTGHADRLN
                     RDLSHPFGSEGYAREELRAEIASLMLSQELGVSFNPGQHASYVASWIMVLQDDPMEIM
                     RAASDAEKIQGYVMAFDQVREIEQQNEAAVSISDRQNIDEASLAEEKNLINAVDKNDV
                     AAIINFMAEEKMHEGLKRFSLVISEMDDATFQNAFWQRHQKPENGESFMENLNAAKEA
                     LDEAIEQSSYRTVEEQIRQFLPEQDKESVRVVSDERVYVQIPYADREQAKMLGAKWDK
                     EEKSWYIPPGVNQEPFRKWLDSATNQAKTVTDNTPAIAKTLIDVPFREKNEARALGAK
                     WDKDQKSWYIPEGVNTALFARWMQPKQEALAEEAVPDDTPQEEAVECQVAAIQQEYQA
                     AKTEEAPAEENASRVYLAVPYEEKEEAKALGAKWDKANKSWFMESSNANLPAASKWLP
                     EKQQTQSMPAMSPVEEFADKLREMGCVVANGHPFMDGQKHRIETVGDKKGERSGFYVG
                     HLDGHPAGYIKNNRTGAEVKWKAKGYSLSPEEKAKLAAEAAQKLQMRSEQKKQAQEAA
                     VGRIKYYMESYLPVQTGTPYLNAKGIKPQPGIFTDREGQKTFIPVYNAAGELRSMQYI
                     QADGTKRFAKDSEQEGCMHVVGQQDLAKAKTIILSEGYATAASIKEATDDTVASVAAF
                     NSGNLPLVAKVLSAKYPQAQFLVAGDDDLAVEAKQGNNPGKEKALEAAKILNCRAVFP
                     VFAPGEQSSEHKAFTDFNDLAQKSKFGREGLAKQINEAIHARPANELQTLKTRGLQEE
                     ASQDRPVEQTKRQQRATTRKTASRGSR"
     gene            complement(28424..28588)
                     /locus_tag="ACFI8B_000577"
     CDS             complement(28424..28588)
                     /locus_tag="ACFI8B_000577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833865.1"
                     /translation="MIAEKVADLNNPGMEVDFDPAEAEALGAFEETAMDAETARDSVA
                     DLSREVAEGA"
     gene            complement(28610..30805)
                     /locus_tag="ACFI8B_000578"
     CDS             complement(28610..30805)
                     /locus_tag="ACFI8B_000578"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC3833866.1"
                     /translation="MSRLYIAEKPEVARAIAAALGVGIERRNGYFRCSNGDNITWCYG
                     HMLALCDPEDYDSKYGNWKFEDLPIAHIPWRKKPLSDKKEQIGVIRDLLAECTEIVHA
                     GDPDDEGQLLVDELLEFLNCAKPVKRVLINDNNERSVKRALSAIEDNAKFRGLSQSAE
                     ARSVADQLYGYNMTRAYTLKAAEQGYQGVLSVGRVQTPILGLVVRRDRENQSHVKTHY
                     YHVYADLDVDGVRFTARYVPNKDQDPIDDKGRLSDPLFARQIAEAASGQPVKILAAKT
                     SAKRQSPPLPYNLLKLQADCSRKFGLKPDQVKDIGQSLKDKHRLITYHRSDCQYLNDE
                     HFDDAADVLDAVGKTASVFKNAIASADTKIKSRAFNPDKVSAHHAIIPTVKTANMDDL
                     TEDEKRVYLLIARAYIAQFWPDHEYNQTDIEAEIAGKKFVCRSNVTVKPGWMALYKND
                     QGNAETATDPEDLSVDLQMLKPGNSGRCLTAEETQFETKPRPLYTMDTLLNDLTRVAK
                     YVQNPKLRELLIEKDKGKEGEHGGIGTPATRDEILRNLLDKGFLLEKGKNIVSSEIGQ
                     QLYDALPDQAKFPDMTALWHEQQQHIVQGGLDAQRFVNSLMEYISGEVERVGREGISL
                     NVKKYPCPECGKPLRRMRGSKGFFWGCTGYKDGCKVSFSDKAGRPVLAPKKAEPSSKF
                     KCMACTHPLVRRPGKQKGTHFWGCSNYPECKQYYPDAGGKPDYSKHTKG"
     gene            complement(30808..31344)
                     /gene="traF"
                     /locus_tag="ACFI8B_000579"
     CDS             complement(30808..31344)
                     /gene="traF"
                     /locus_tag="ACFI8B_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HFC3833867.1"
                     /translation="MRRLFDRAMLAMCLTAALLVTGYGLLYAAGFRINDTRSVAKGVY
                     RISGEAVRRGSYITFCPPDTEVFRMARRRGYIRAGFCPENYSGLMKFVAGIPGDNYRF
                     TDEGLLINGRLLGGTRPLWQDGLGQPLPVFEGEGRLKDDEYILIGNAVANSFDARYYG
                     IVRREHIRDVVKPIWIEE"
     gene            complement(31341..33230)
                     /locus_tag="ACFI8B_000580"
     CDS             complement(31341..33230)
                     /locus_tag="ACFI8B_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFC3833868.1"
                     /translation="MFRSKNKRNNAVGHQNQAQATGSGRLVNFICLVLVLLAGMGAAT
                     QYFAHQFQYHSQLGYGIGKFYMPWSILIWYGKWHGRYEQQFMTAFSIMLMVVAVGMIG
                     LLVWNIVRKNTNKAHESLHGSARWADRKDIEAAGLLAKKGEDSDGVYVGGWVDKKGTL
                     HYLRHSGPEHVLTYAPTRSGKGLGLVLPTLLSWPHSTFITDLKGELWALTAGWRKKYA
                     HNKVIRFEPASAQGSAAWNPLDEIRVGTEYEIGDVQNLATMIVDPDGKGLNSHWDKTA
                     FALLTGVILHALYKARDDGGVATLPSVDRMLSDPEKPVGDLWVEMATYPHADGRPLPV
                     ISSAGQDMLDRPEEEAGSVLSTAKSFLALYRDNVVAANVSRSDFKIKDLMNDEDPVSL
                     YIITQPNDKARLRPLVRILINMIIRLSADKMDFENGRPKVHYKHKMLAMLDEFPSLGK
                     LEIMQESLAFIAGYGIKCYLICQDLNQLKSRETGYGPDETITSSCHVQNAYPPNRIET
                     AEHLSKLTGITTVVKEQITTSGKRVSAMLGQVSRTTQEVQRPLLTADECMRMPGPVKD
                     ARGLITQAGDMVIYVAGYPAIYGRQTLYFKDPVFEARSKVPPPRHTDRTQKKTANTPS
                     AFDTL"
     gene            complement(33247..35619)
                     /gene="traI"
                     /locus_tag="ACFI8B_000581"
     CDS             complement(33247..35619)
                     /gene="traI"
                     /locus_tag="ACFI8B_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="HFC3833869.1"
                     /translation="MRKANLSSFEGLVTYLAGSQGVANRVGKVEIHNYESSDLRWAVQ
                     ETLAVQDQNKRSAADKTYHLMVSLAGGEFLTDEQLSRIEQSMIEALGYAEHQRISVVH
                     RDTDNLHFHVAINKVHPEKKTIHEPYNDFKILGRECRKFEEEYGLQRVQRGGRGNKKI
                     DMEEVAGIESLIGWIRRECGEQIKEAKTWEQLYHVLSDHGLEIKLRGNGFVISDGEHH
                     VKASSVDREFSKSALEKRLGAFDQKTGVKAKPSKTYREKPLSKANVDDLFRQYKEEKE
                     LQKSRRQDFFLHLAQNRDMEIGRIKEKYAFRRSLIRHSSSGIARRILYRMLFRQMKDE
                     IRSVRTDYARRRAVFFEQNKGFAWADWLKHRAAEGDVRALDALRRRGKDAGYKGNRFV
                     GKESGGFAGIKVDTVTKKGTVIYAEDGMTIRDTGSVLKLAAIPDDKAVSRALEIAIGR
                     YGKVLTIGGTRHFQDKVARIAAAKGLDVSFTDGRLNHLYQQYVKEYEYVRERTDRYRQ
                     ADGTGRADGRAGQQRGGAGYAGGKPRRYRWHGYDASRNRGFHAGISGYPIPKLSDSGG
                     IGAGRGQSASAGSDSLRGMPERSLVRIQGRTQVLLPRDVSSHLEQQGSANYQAVRRTI
                     HRTGGVAEIRQMQAVVGLVAKPGKKPPGFRRGSLRGMEGLAEIKISSAAVVEIPKNPE
                     PAQENERLTHSLAPIDKYIAERQEKIDKKFDILNHRKYNPSDSGVMLFRGIRNIDGQA
                     LALFQPKEVEEILVMSVTPYVSRRLSSVKLGSEVKVTPTGQIRPVKGRHR"
     gene            complement(35642..36019)
                     /locus_tag="ACFI8B_000582"
     CDS             complement(35642..36019)
                     /locus_tag="ACFI8B_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFC3833870.1"
                     /translation="MAGARKRPKYISVYVSEEEWKTIESLAKSVGLSQSAYLKLLGLG
                     YKPASVYDGEAVKALAKINADQGRLGGLIKMWLANDERLMMFDHDQLAKAIFGVLEQI
                     GEMQESMLEIVRSRRILAAKKIG"
     gene            36069..36788
                     /locus_tag="ACFI8B_000583"
     CDS             36069..36788
                     /locus_tag="ACFI8B_000583"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HFC3833871.1"
                     /translation="MASLAEQDNPLSLPANKDCDVHVHHISCPNSGVHPNSGVQYAKD
                     YTPNNSIYRTYFDDKPPKNALSQTKNDLLYRIIVFKTILLGSFGKALSKNWQSPIEMI
                     QMGKIEKLVLAELAAMQKKGSSAGNKARFLAMQEDIKILLVDRNIPVISVWRILSEKK
                     YVPYSYETFNRYCKMYIFKSDTTAADQTDTAQSTQEKPDSIRTDKEPPSPASEPIIAG
                     GNTKSSSGFTFNNNPDGKDLF"
     gene            36789..37514
                     /locus_tag="ACFI8B_000584"
     CDS             36789..37514
                     /locus_tag="ACFI8B_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFC3833872.1"
                     /translation="MAIIHISLQAKGGVGKSFLALMLSMFYRHIERPVKIFDTDPANA
                     TLFGFPALEAEKFEIMKKDDIDKRLFDRLFSQILSATDDVIIDVGANAFIPLCSYIIS
                     NNLITMLTQEGHQVIIHTSIVGGQNLKHTVNGFDALCSQFPESTGFVIWLNPFWGEIE
                     FEGKSFEKLKVYEKHKSRIRSIIKIPEWDASLHGRDLAAMLEKQKTFDEALASEDFSI
                     FERQRLNIIKNEMFQTIETAQIV"
     gene            37522..38004
                     /locus_tag="ACFI8B_000585"
     CDS             37522..38004
                     /locus_tag="ACFI8B_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833873.1"
                     /translation="MDEKVKAILATIVEKNGIALSPKDPIMVVFTALEYMLSEQHAEQ
                     KALTVLFKAQVEEATGNWIQSVHQQTEELVSQQLNTTKEQAKEMSKLLLHETDRLLEQ
                     RITDAVKDFEGKLYGINDNAMNAVELRIKETRNTAILNLTAALIVLLAVIILAFKFTF
                     "
     gene            complement(38061..38597)
                     /locus_tag="ACFI8B_000586"
     CDS             complement(38061..38597)
                     /locus_tag="ACFI8B_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncP plasmid survival protein KfrC family
                     protein"
                     /protein_id="HFC3833874.1"
                     /translation="MNEDKGLVAKAVAAETEQEELFESRQVIEETFAGVLNTYVLEKT
                     EQVERIEEKLEQLISGQMAKLQALQNARPGMLSMPSKKQTWSANIAVAQSRLNRLHDR
                     LEDVREIRDGMALGVPKIQELAARKLRMEEPDLAKKFDDVQTAVRVHNLHEKQRKEKE
                     QQKRQAGLSLSLGQKIVR"
     gene            complement(38634..>39039)
                     /locus_tag="ACFI8B_000587"
     CDS             complement(38634..>39039)
                     /locus_tag="ACFI8B_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KfrB domain-containing protein"
                     /protein_id="HFC3833875.1"
                     /translation="TVLLELFTSFLKIFSRMEAEKQRVLVLNKQHLFETEKDGKWITQ
                     AVKPAPANMKPGIYLLHTAKSPQDSVRYEGQIVFQNDSYVYQKTRQSIVKYPCSLFSS
                     VPSIGEVVSISVKDKAATVEKAGIGEKKTLTR"
BASE COUNT         9609 a        10394 c         8607 g        10429 t
ORIGIN      
        1 gattttaaaa tgatttaatt taatgaattc tgatcaacct gctgattcgg tattttgcac
       61 ataccggcag cagtaaaact gggcagcccg ttaaaacggg ggggtattat aataaattcc
      121 cagtattatc cttcaataac tgcaacaatc ccggaaattt ctttacaagg aacagattct
      181 gtttcgccgg actcctcaaa cttaatccat acctcatcgc cagacaatcc gcgcttgttc
      241 aaaaccagcc ttgccagttg gccttgatac attacctgca caatagcttt tttcagtttg
      301 tcaggatctt ccggcttttt ctctttcact tttgccttgg atggcttttc atgatcggca
      361 tcagcagaat ttttaggatc cgagtttaaa aactctttca aattccttac cgaactacgg
      421 gtaatctcgt cttcgttaga cagcaaatct tccaccgctt ccggttcttt tttatacaat
      481 gcgttcaggt cattgattac cgtcaaatcg gatactttcc ccgagttgaa tgcgttggcc
      541 agaggttcgg gtaagtccag caggtttgca tgctgggtaa cgaaagcagg ggatttgccc
      601 attttttcgg caatctgtcc ctttttcatc ccctctgcca gcaatcggcc aattacgtcg
      661 gcgatttccc ttggcgttag attatcgcgc tggatatttt caacagcctg atcgatcagg
      721 ctgtaatctt gatcgatata tgcgggaatc tcgctcagcc ctgccagcaa tgaagccctg
      781 taacgtcgtg cgccgtggtt gatgacatag accccttctt cggaactttg acgcaccgat
      841 atgggtgttt tcacgcctct ctcctgaatg ctgtttgcta gttcctgaat ttttccttcg
      901 tcaaattctt ttcttggttg atcggggtct tctttgattg aagcaagggg caggcgcagc
      961 ggcgtccctg atgagacctc tgcattaccc aaaagttcgc tgagattgcc gatgttttcc
     1021 aaacccaaat tactgttatt cataaatttt acccttgcat tttgtttaaa acatagttag
     1081 aaacggcttt catttcctgc gccgcctttc ttgctgccgt tttcttgatt ttccaaaccg
     1141 gcaggctgga agacaacgca tctgcgatac tgctcctcaa tccgataata tgcggtatta
     1201 caatttcatt atactgcatt ttaatttctt ccaaatgcct tttatggcgc ggattcctca
     1261 tatcgacctt acttggcaat atacccaaaa aagccaattt gctgttcact tttctgatat
     1321 tgcctattgt tgcagccatc tgtttaattc cttgtaggct gtatgtttcc aactcgatcg
     1381 gacataacac ataatccccg gccgccagtg cagccgccaa cgtgttacct aaactgggcg
     1441 gagtatcaat aatacagaca tcgaattgcc cgcccgcttt taatttgtcg atgttttgcg
     1501 taaaagactg aacagcattt tgcaaagtca tattctgcac gttagccaaa tcgtttgtcg
     1561 ccggggaaag agccagtgaa acgttgccgg gcatatgtaa atccgcatct tccatatttc
     1621 caaacaaacg gccggaaccg aaaagacacg ttttgtctcg aagagagtaa ctcgcattcc
     1681 cctgcgtatc caaatcaatt actaaaaccc tcagtccaac tgtttccata tgccaggcaa
     1741 gatgaagcga catagccgtt tttcctaccc cgcccttttg gtttgccacc actaaaatct
     1801 tcattcctac acctcatatt ttatttctga acgcgataac aaaagtgagc catcccatta
     1861 cgataccgaa cgtcaagata aatttgccga tataaatcca tgaatcagcc atattccagt
     1921 ggtacagcat ttggcacaaa acatagaaac tgtacatcca agcaaaccat cggacaggga
     1981 aaagcaaata ggcgatagct aacaccatat aaaccacttc aaacattttt tttccagctt
     2041 tggatttaat tctgtccata ggagcctccg tccgatatag tttgtattat aggataaatt
     2101 atcctatttg tcaagcaatt ttagcggcta aaattttatt taccaaaatt tctcaatacc
     2161 cacatacaag ctcaataaaa cccaataggc atatgtgatg gatgattccc cactgatgaa
     2221 tctcctgaaa gctctaccat tggtgtcttt caaccctaaa tacctgctaa cttgtttttg
     2281 agtcattccc gttgctaacc acaattcttt gacctcatca gccgtaggga ctgcccattg
     2341 cggaaaccgg tggaaacatt ctggacggat attcaggtct tttttttgta tcaagttatg
     2401 ctcttcaata ttttgaagat gccatttctt tgcttgaaat gcttggtaag gagtaagtaa
     2461 tacggtggta gaaattaaat cggtcatttg aaagttctcg gcaatttacc cttggagtaa
     2521 aatgaaaaag atctaaatcg cccgtagggc gacagacgaa tttatgaaat tatctaaaat
     2581 cgccgtaggc gacagcaaat tgataaaacc ggcttttcgg tcgcgaaata tggttataaa
     2641 tggttagata atggttaggg caaaaaaaag cggttttttt caattaatat tcagtatgtt
     2701 gcaaacatgg cggcatgtgt aaatccgcga tttagcatgt gtaaatccgc gatttagcat
     2761 gtgtaaatcc gcaagggaat taggatattt tgtcctaaaa gcatgtgtaa atccgtaaat
     2821 tttgctcata ttttaggcga taccgaatgt tatgcacagg ttatccacag ttttgatttt
     2881 taaaataggt tttttatatt tattttgtct gtttgttttt taatttttat cgcatatctt
     2941 gacctgcata ttttggtatt tttgtcaaac cggccatcgg catgtgtaaa tccgcaaatg
     3001 gcatgtgtaa gtccgcaaac agtgaaaatt tgctgttttt tggcatgtgt aaatccgcaa
     3061 atggcctgtt tggggcatgt gtaaatccgt gcttgtggat aattttaacg ctcgacgata
     3121 atcttcccgg ataatacaaa ggcagttttt accaactgcg cctcggtgag ttcggcacaa
     3181 atctttttag cattttgatt ttgcgttttc gctggggagt tgatttggtc gctgccgcac
     3241 attgctaaaa atgcaggaat aggtataggg ttgggatgtt tgtggctaaa gacataatcg
     3301 gtgagccgcc tagccattgg agaaagtttt ttgtattgac tccaagggat gttggaaaat
     3361 gtgtttccgg caaaaagcac gattagtttt ttatcgatgc tgactttgta acgtgtcggt
     3421 tcgcctttga tgttttcaat tccgacataa ttgtcaataa gggaaatacc tccgctgacg
     3481 ccgtaggtat ttccgttttt gatatagatc tctgtcgctt tcattctgct caaagattcc
     3541 cggacacgtt tataataggc tccgctggta tcccatccga tgtccttaat aagctgcctg
     3601 atgtcaaatt cgacgtaatt ccccagaggg aaacgacggc agtaatctac aagctgcatc
     3661 catatcagct cgtcatccac tgaacggagc tcggtacctg tgtaaaggat ctctactttt
     3721 tctgagatat ggaaaagttt ttcacgggag aaagtttttc tcggcacccg atgatttctt
     3781 gctgtaaaca aacatgatcg ggcgaaatcg ttcggcatcg ctctatgctt tgaaccaaag
     3841 atagtatttc caaacaaatc ctctataata aggttgtcgt ctgtcggaat gccttttgat
     3901 ttcaagtatt cttccctttg ggcttcatcc ctgcgttcgg cgactaaatt caaataagga
     3961 agcacttcct gaacattttt cataatttat cctaagtctt gttcggggtc gtagataaat
     4021 gaggggtctg atgattcttc ccgtccggca gggggtttga ggttgctgta ttcaatacgg
     4081 ctggcaatca tctggtcttt atagattttt tgcccatctt tttcatagtt gttattgctt
     4141 atccttcctt ctatcaggat aagggaacct ttgccgcaat acctgacggc gttcttggcc
     4201 tgttctccat atacatctat ccaaaaatag tttgtggcct caactttctt cccatccttg
     4261 gtgtaaaaat agttttctgc caaaggaata ctggttgtaa aaactcctgt tgcagtttgc
     4321 tccaaagtca aatctttggt tgttctgcct gaaatgatga atttattcat tctgcctcct
     4381 aggttacttt gctatactta cggttaaaaa ttttagcggc taaaattcca tgagttattc
     4441 gaaaatattc ttcactaatg ttctgcgtct tgcatccgaa aagaatctgt cccaccaaga
     4501 tttggcagaa agggcgaatt tgtccccgtc ttcaatgtcg ggaattacaa gagggcaggg
     4561 caatccgaca ttggaaacca tgtcggccat tgcttacgcc cttgatgtcc cgttgagcta
     4621 tttgctcgaa caccacgatc ttgatccgga gagcctttcc cttctagatg gaagtcaatc
     4681 aacactcccg cctaattacg agaagattac ggtcattttg ccgcgttata aggcattcca
     4741 agttaaaaag tggaacgatg atgccttaaa gattttggca aaagaaacca aatagcaaca
     4801 ttcattaggt tattttatcc catatctgcc agatatgggc tttttttttg gctcttgaac
     4861 aattagactt tacattttat tgaattcaat atattgtatc aactctattg tatggttctc
     4921 ttaatgatgg aaacgacgga aacagcagac tcccgcaaag aacgggcatt gaacaaacta
     4981 aagcgtgatt tgggaaatga gtttttggaa aagttcatga atccaaactt ggttgaagtg
     5041 atgttcaatc caaaacagac cggaaacggc gtgtctgttt actgggaaga gcaaggcaag
     5101 gagatggtct atgacgagag gttgagcgat tggttcaaaa cctcccgcgt ggaagcggtt
     5161 attaaaaccg ttgccggtta tcacggcaaa gtaatcaccg aagaccttcc tatcttggag
     5221 tcccaatttc ccatcgatgg gagtcgtttt acggccatct tgccgccggt aaccgcatca
     5281 ccctctttta ccatcaggaa aaaacctgcc cgtgtattca ctttggatga gtatgtctca
     5341 gcaggaatca tgacggaaag ccagcggcag accatccgca atgcagtcag tgagcacgga
     5401 aacatcttgg ttatcggcgg aacaggttca ggtaaaacca cgttgattaa cgccatcctc
     5461 tatgacatgt cggagcagaa tcccaatgag cgtctctaca tctctgagga taccggggaa
     5521 atccaatgcc attcgcaaaa caaagtgcag gtttacacca cgggaagcat cgatctaacc
     5581 aaacttgtga aaaccgcgct ccgctaccgt cccgacagaa tcatcgtagg cgaagtaagg
     5641 ggggcggaag ccttggatct tttggatatt tggaacacgg gacacccggg cggcatcgcc
     5701 acactgcatg caaacaatgc cgccttggga ctggaacgct tggaaagcct gattacccgc
     5761 aatcctgccc atccggcagc gatacggccg ctggtggcgc aagtgatcca ctacatcgta
     5821 tccatcggca aagacgagaa aaccggccgc aaaatacgtg agatcatcaa aattaagggg
     5881 tttgacgaaa agtcaggcaa ttatgtgttt gattaccttt aagaggaaca gggaggcaat
     5941 ttaaacagcg ggattgaata ctgatgtttt cataagcaac gaacgatttt ttaacaacca
     6001 cgttatatct gtgaggtaaa tatatgactc tgagtcaatc gcaacaaaaa tggctgttct
     6061 tcatcggact ggccgtcttc ttactgttaa tgtcctcgcc cgcgctcgcg tcgcaagggc
     6121 agggtggcgg tctgccctat gaaagctggc tgaacaatct ccgccagtcc gttaccggcc
     6181 cggtggcttt cgccctgtcc atcatcggca ttgtcgttgc cggtggcgta ctgatcttcg
     6241 gcggcgaact gaacgcgttt ttccgcactt tgattttcct tgtgttggta atggctttgc
     6301 tggtaggagc gcaaaacatg atgtcctctt tcttcggcgg cgcggaagtt accgctgccg
     6361 tgcaggccgc acagcattac cttcttccct tggcgtcgaa aggataaaac catgagagcc
     6421 atccgcacga tacccatccg cagagtggca aaccgtccca acctcttttg gggtggagat
     6481 cgcgagatgg ttcaggtatc ccttttgatt acttttgttt tagtggtggc ggcgcaaaac
     6541 acctatgctt tcgtaatcgg cttggtattt tggttttttg cgctttacgc actccggaaa
     6601 ctggctaaat acgatccttt gctgcgtttt gtcggcctgc ggtcgattac caagtacaaa
     6661 cgtttttatc cgccccactc cacaccgttt agaaacaata cttcaaccca aggaaaccgt
     6721 tacaaatgat cattgggatt atctcggcca tatcggtttt gggcttggtt ctgtgtacgg
     6781 gtctgttcct gaaaatccgc gccgttacca atgccaccaa actcaaacag caccgctcga
     6841 aagaagaagg gctggccgat ttgctgaact atgcggccgt aatcgatgac ggcgtaatct
     6901 tagggaaaaa tggctccctg atggctgcat ggatttatcg cggcaaagac atgggcagca
     6961 gcacggtagc ggagcgcgaa gacgtatctt tacgcatcaa tcaggcgttt gtcggcttgg
     7021 gcagcggctg gatggtgcac gtcgatgccg cacgccgtcc cgcaccaaac tataccgagg
     7081 caggcaggaa ctactttgac agttacgtta cagaggccat cgataacgaa agacggcagg
     7141 catttgaggc catcggcacc ctgtatgaag gtttcttcgt cattgtcgct acctatttcc
     7201 cgcccttact ggcagaaaaa aaggtcgccg aactgatgtt tgatgacgac aacgccaagg
     7261 aaggtaaacg cgcccagcaa gaccgcacac tctctcattt caaacaggct tgtgcgaatc
     7321 tggaatccaa cctgagcatt tccttggagt tgcaccgtct ttgcgcccat aaagtcgttt
     7381 tggaagacgg catggaaatt acccaagacg atttcctgcg ctggctgcat ttctgcatta
     7441 ccggcaaaaa ccatccgatc aaccttccgg ccgcgcccat gtacattgac agcctgattg
     7501 gtgggcagga actctacacc ggcgtcgtgc cgatgatcgg cgataagttt atccaagtcg
     7561 tcgcgctgga cggcttcccg atggagtcct acccgggcat tttgacgcgg ctcggagaaa
     7621 tgcctatcga gtaccggtgg tcgaaccgct tcatcttcat ggatcagcat caggcggttg
     7681 ccgatttgga gaaataccgc aaaaaatgga aacagaaagt acgcggtttc ttcgatcagg
     7741 tgttcaacac ccattccggc tccatagacc aagacgctca ggcaatggtg gatgacgcgg
     7801 aagccgcctt ggccgaaacc aaatccggct tggtggcaca gggttactac acaagcgttg
     7861 tcattttgat gcacgaagac cgggctttct tggaaagcat ggcaaggcag gtggaaaaag
     7921 agatcaacac tttgggcttc aatgcccgcg ttgagacggt caatacgatg gaagcatggt
     7981 tgggcagcct gcccggccac ggaatcgaaa acgtccgccg tcctttactg aatacgctga
     8041 acttcgccga catgattccg accagcagca tttggacggg cttggaggaa aacccctgcc
     8101 agttctatcc gcccaattcg ccgccgctga tgcaggttgt taccagtggc tccagcccgt
     8161 tccgtctcgg tctgcacgtc ggcgacgtcg gccacacttt ggttttcggg ccgactggcg
     8221 caggtaaatc taccctgttg gccactttgg ccgctcaggc cgaccgttac cggaacatgc
     8281 gggtattttc tttcgacaaa ggccgttctt tggaaacgct gacccgcgcc gtcggaggat
     8341 cacactacaa cattggcgat gaagacaaca cggcttccgg tttgtctttc gcgcccatgc
     8401 agcttatcca aaacacgtcc gaccgcgctt gggctttgtc ttgggtggaa gcggttatgg
     8461 aattgaacgg cctcaaggtt acgccccaac agcgtaacga aattgaaacc gccatcaaaa
     8521 acaatgcagc cgaacagtcc tattcgcttt ccgatctttc aaacaccctg caagacgtag
     8581 agatgcgcga agtattgaaa gcctacacca tcgacggctc gatgggttat ctgctcgatg
     8641 ccgaacagga cagcttgagc ttctcccgct tcacctgctt tgagatcgaa gagttaatga
     8701 acctgccgga aaaatatgcg cttccgatta tgctgtatct gttccgcaga atcgaaaaag
     8761 ccctgaccgg cgatcccgca gtcctgttcc ttgacgaagc atggatcatg ttggggcatc
     8821 ctgtcttccg ggaaaaaatc cgcgaatggc tgaaagtgct gcgtaagaaa aactgccttg
     8881 ttctgctcgc gacccaaagc ctcagtgatg cggccaactc cggcatcctc gatgtcctga
     8941 acgaatcctg tccgaccaaa atctacctgc ccaacgccca cgctagggaa gaggacgctt
     9001 cggaactgta caggcgcatg ggcttgaatg cgcggcagat tgagattttg gccaccgcca
     9061 ttcccaagcg gcagtattac tacacctcgc cttacggccg caggctgttt gaactggctc
     9121 ttgacccgct gaccttggcc tttgtcgccg tatccgaccc cgacaccctg aatgcaatgc
     9181 ggcagacggc cgaccaatac ggggaagact ggccgatccg ctggctgcaa aaccggggaa
     9241 tcaatatcca taactatgtc gatgtggaca aatacagaag gaaacactga tgggactgaa
     9301 agacattttt cgcgtcaggg aaaaagccga ccgcgaaatg accgaaaacc cctatttgaa
     9361 cgccaggaaa caatggaact tcatcatggg cgacatggtt tcgtcgcgcc tgctgtggca
     9421 atgggcgggc atgaccggcc tgctgattgg tttgggcggg gtagggggca tgatttacct
     9481 cggcagccag tcgaaattta tcccttatat cgtcgaagtg aacaaattgg gcgaaaccgt
     9541 ggccgtggcc agagccgacc agcctcaggc aaccgatgag cggattgtcc gcgccatgct
     9601 cggcagcttc atcggcaatg cccgtaccgt aaccaccgat atagccctgc aacgcaaata
     9661 cgttctcaat acctatgcca tgctgaatcc gaaagacgcg gccgcagcca aaatgaacga
     9721 atggctgaac ggaacgccgg agtcttcgcc gttcaaacgc gcggaaaaag aactggtcag
     9781 cgtggaaatc aagtcggtgc tgagacaaag cgacaactct tggcaggtgg actgggtgga
     9841 aaccactcgc gcccatgacg gcaaattact caaaccgcct cagaatatgc gtgcgctgtt
     9901 gaatatctac tacgttccgc cgacaacaga gcagcagatt attgtgaacc ccatcggcat
     9961 gtttatccgt gatttctctt ggtctaacga actgtaacaa ggtacagcaa tgaaaagaca
    10021 gattttatcc ttggcgttaa tcaccgcttt gtccacttcc gcctttgccg acagctacgc
    10081 cgacaaatat tttgaaggca gccacaaccc gaagctgaac cgccacgaac aaaaaggctt
    10141 gggcatcgcc aaaaactact cttctgacgg caggatggcc gtaccgcccg ttcccggcga
    10201 taacggccgc atcacctttg ccttcggctc tgatccgtct gtcgtctgtg ccgtaatgca
    10261 ggtctgcgac attgccttac agcccggcga aatgattaac gatctgcacg ccggggatac
    10321 cgcacgctgg aatatcactc cggcaatttc cggcagcaac ggcaacgaaa gaatgcacat
    10381 cttggttaaa ccactggatg tcggtttgca aacgacgctt ttcatagcca ccgaccgcag
    10441 aacctatcat atccgcctca aatcacaccg tacccgttat atgcctgccg tcggtttttc
    10501 gtatcccgaa gacaccgccg cgcgttggcg ggcgatccgg gcaaaccaag cccggcaagc
    10561 cgaagctcag gttctgccca ccggagaaaa catccaaaac ttggatttca actacaccat
    10621 cagcggccac aatccgtcat ggaaaccttt gcgcgtttat aacgacggca cccgcaccat
    10681 catccaaatg ccgccgactt tatcccaagg ggaagctccg gccttgctgg tcgtccgtaa
    10741 cggcgaaaac accttggtca attaccggct tcacgaagac cgctacatcg ttgacagcct
    10801 gtttgacgaa gccatcatga tcaccggcgt tggccgccat caaaccaaag tcgttatcag
    10861 acggagccga tcatgaaatc aaatgtcctg ttcgtcgcgc tggctttggc cgttggcgga
    10921 tgcgccaccg ggcaatacgg ttctttcgtc cgttcagacg ccgtatccgc ctcagtaact
    10981 caagatgtag tggaaaaact ccaagaagaa tttccggcgg ccaacaccgt tttcttcctg
    11041 aaccgcagca aatccgatac cttcggtacg caactggatc aggcattgcg tgaaaaaggc
    11101 tttgccacaa ccgcccaaca gggcgaaaac gaaaaatcac tgaattacgt cttggatcag
    11161 gttgattccg atatttaccg ggtaatgatc tacattgacg gcacgcccta ttcccgtgcc
    11221 taccgccaaa ccgaaaacgg cgccgtggtt ccgaccggtt cttggactaa acgtggagac
    11281 tgaagcatga gccaaagcac cgaagatctg ctgaacgcat cccgttcccc ttcgatcgca
    11341 ccggatactt ccgaccgtcg tcccggtgtg cgccgcctga acaacattcc gatctatatc
    11401 gtcggcggtg ttgttctcgg attcgccctg ctgattgcac tggtggcagc aactaaaggc
    11461 caacagtctg ccgggcaggc aacgcaggga gacggtaagg atgcaacaag aatggccgat
    11521 tccctattga acgaactgcc tcaggaaggc ggtctgatta ccgccaaagc atccgaaccg
    11581 gccatgcccg ccctgcctga cggcatggcg tccgcgcctg atttaccggc atccgcgccc
    11641 acatctttac cgatggttcc cgatatggcc aaccttccgg ccacacctcc cgaagtggat
    11701 caggaagcgg aacaaatccg gcaggcaaaa gcgcagatgt tccaagcagc cctagctgcc
    11761 ccaaccaaag tggatgtcgt cgcatcgact tctcggggtt cgtccggcct atatactgct
    11821 gccttatctt cttccggctc aggtacaccc aaccgtagcg agatgctggc tcaaatcgcc
    11881 gaagtgcagc gcatgtccga gaatgccggt tccaatcccg ccgaagtgga gcgcgtttac
    11941 aaccaacaac tggccaatgc ccaacagatg gccgccgcag gtggtcagga cggcgtacaa
    12001 ggggcaggcg gcatgcccgg cctgaacaca cctccgactc ccggcaatat cccgagacaa
    12061 acggcacaaa acggtggaaa caactggcgg cttgagagcc aagtcgaacg tccgaacgcc
    12121 tacatgatcc gcagcggtgc ggttatccct gcaaccctga tcagcggcat caattccgat
    12181 ctgcccggca tggttcaggc acagatctca caaaacgtgt atgacaccgc caccggccgt
    12241 catctgcttt taccccaagg atcgcgcttg gtcggcactt acagcagcgg catcaatctt
    12301 ggccagcgtc gcctgtttat cgcgtggaac cgcattattt tccctgacgg taaagccttg
    12361 gacatcggcg atatgcccgg agccagcggc gcaggctacg ccggattcag cgaccgcatc
    12421 aacaaccatt acggccgtct gtggggcaat gccctgatgc tctccctagt cggagccagc
    12481 atcacttacg gcgtggacaa aaacagtaac aacaataaca acggcaataa caacggcaac
    12541 aactccaccg tgcgcggagc cttgtcggag tccttgggtc agaccttcgg acaggccgtg
    12601 tcccaaagta ttcagaaaaa catgaatgtt tccccgacgt tagagatccg cccggggtac
    12661 cgcttcaatg tcattcttac taaagacatt gatttctcta agccctatcg ttcctttgac
    12721 tattaacagg agagcattat gcaaaaacgt cttttaagca aaaccaagac cgtagccctg
    12781 tctttggccg tttccgtgtc cttggggctg tccgcaccag catacgcagg catcccggta
    12841 acggatggcg gcaacattgc ccaaagttcg gtctccgcca ttcaaaatgt ggcgcaaacc
    12901 ctgaagcaga ttgaggagta ccgaatccaa ctgaaacagt acgaaaccca gttgcggaac
    12961 tccttggctc cggcggctta tgtttgggat caggccaaca aaactatggc caacatgaag
    13021 gccgctgtcg cccgcttgcg agcctacaaa cagcaactgg gtaacatgga agagtacctg
    13081 agcaaattcc aagccgtgga cttctacaaa agctctccgt gctttaccgc caaaggttgc
    13141 tctgcggccg accgcgccaa actcgagcaa aactacgccc aattgtcgcg catgcagaaa
    13201 ttatctaatg acggcctatt aaggagcatc aataacgaga tcgactccat cagcagagac
    13261 gccaacacct taacccaact ccaacgaaac gcccaatcag ccaccggcca gttggaggca
    13321 ctacaatacg cgaaccagtt ccatagcgct caagcgcatc aactgcttca gttgcgtaat
    13381 atgattaata ctgagatgca ggcaaatgct gcttatcgtc aaagtcaact tgatagagag
    13441 gctagatatg aagctgctcg taaacaagtt aggacgtcgg atattttgaa acacccttat
    13501 agaagagggc aataaaaata gggtttctaa tgtgtaataa acattagaaa cccactctaa
    13561 atttgaaaga ttacctcatg agaactttat tttttgtatt gctaagtagt ttcatgcttg
    13621 ttgcgtgcca acaaaaggat gagaaccaac aacgcagaga agaactacgt aaagtagatc
    13681 aggatgtcct aaaacggcca tacaaaagga aagagtagtt attttcttat gcaatatata
    13741 acccaaatta tgaaaaaaaa atatatttat tattttatat tttttataac gctctctatg
    13801 ccaattatct ctcatgcagc aggtcaagga ggagttttag atgaggcatt atcacgctac
    13861 gaaggtaaaa tcggagcgtg ggaagcagca ttttataaag ctggattatt tatattttgg
    13921 tcattatctt caattagtat tgtaatgact ggagcccaac tgattttcca gagagataat
    13981 atcagcagtt tttttgctga atttactcgt ttaattttat tcttaggatt tttcctttgg
    14041 attatcacaa acggaattaa aattacgaca tcaattaaag atggtttaat gaaacttgca
    14101 ggacaagcta gtagaaatgg tgagcagatt acacctagta gtattgttga cataggtttt
    14161 aatttatttg acaaaattgt tgaaaatagc tcaatctggg atccaattga tagtgccact
    14221 cttattgtta ttggtttagt tattttacta atcttagctg ctgtcggaat taacatgtta
    14281 ttaatgttaa taacaaccta tattgtcatc tattcaggaa taattatctt gggattcggc
    14341 ggtggacgtt ggacatcaga tatggctgtc ggatatttaa aacaagtatt aaacttatct
    14401 ttacaattag cagctatgat tctaattatt ggaataggtc aatcgattgt tcaagacact
    14461 ataaacacta taggaacccc tggatttaga gaactagcag ttgttctagt tcaaacagct
    14521 ttacttgttg gtttagtcgt taaaattcct ccaatgatag gttccctagc aggaggagca
    14581 ggaagtggag gaattggctc ctttggtgta ggtaatgcta ttgctgctgc tgctatgttg
    14641 gcaggtgctg ctgccggagc aggtgcggca ttaaaaagct tagccgtaga agctggtggt
    14701 ttaaagaaag catttgaagc tgcaaaagca ggcttgggag gttcaaatgg ctcatcagat
    14761 tcaggtagtg ggaagatgga tagcgcagtt gatgccgttg caggtgcaaa tgccggagat
    14821 aaatctaacc aagatggaaa accactgact gctgagtcca gtggtaactc agaaaatggt
    14881 agtgaaggat ctactacagg aaattcggga ggtaatccat caggctcctc cgaagaaaat
    14941 acaatttcat ccggaagctc ctcaagttcg ggggaagcaa attcaacaaa tacatccgct
    15001 tcatcaggag cggaaggtag tgccgaaggg gaggctactt catcagagac atctcaatca
    15061 gcttctgatt cgccaagcaa taatagcgga gaggcgactc aaacggctga caccacacca
    15121 aaaacaacta ataccggtgg aggaagtcct cttgctactg cgatgggtgg taataaagta
    15181 tcaaattggc aggcggcaaa aaacgttgct gtcggccttg ttagcagcaa atggaacaat
    15241 gccgtcaata actctgccgg tggccgccta gtcaaacact gggaggacga aaagaaaaaa
    15301 acagaagacc ccacaaaaaa tccccaaaaa aaccctgaag gagatgacaa tgcttaaaaa
    15361 aacgcttatc tgcatattgc tggtcggttc cgtacacttt gctatggccg aaagcctcaa
    15421 cgatgcgctg aaccgcagcc gttcgggaaa ctcgaacaac aacaggccgc aggttgaaga
    15481 cttgttcacc ggtgatgtaa agctggcctg cgaagccacg ttatgcctgt ccacgggcaa
    15541 ccggccgagt gaatgccaac cggccatcca ccgttatttc tctatccgcc acaaacggat
    15601 gggagacaca attcgcgccc gacacaactt cctgcgttta tgcccggcat ctaaagagca
    15661 aggcatgctc gaactggtta atgccattgc caacggtgcc ggtcgatgtg atgcccggga
    15721 actgaaccgt atcaaccgtt ccacttatat caagcgtgtt tgggtcggat caagacggca
    15781 tggagactgg caagaggtca aagtccccta tatccgtaac gccaaacctt cgtactgctc
    15841 tgcctacttt gaaaacggct ggacacagct tggcacacgc tatgtaggtg aagagaaaaa
    15901 tggcgggaaa tgggttgact aatggtttat gtggccgacc tgcccccatt tatgcaggaa
    15961 cgtgttacct gctcgattgc tgcggctgaa aaataccatc tgccgccaca ttacctgttg
    16021 gccgtggccg aacgcgaaaa cggtcgtccc ggtcaacagg tacgcaatac caacggcacc
    16081 tatgatgtag gcgcgatgca gattaatacc gcctatatcc gcaccctatc ccgttacggt
    16141 gttactcagt cagccgttgc ccaacccgga tgctatgcct attacttggc cgcatggcgg
    16201 atcaagcggc acatcgttta tgatagaaag ggagatattt ggacaaagat cgcaaactac
    16261 cattcctata cgcctaaata caacgcaatc tatcgcgcag ccatcattcg gttggccgaa
    16321 aaatgggaac ggtggcttgc cggtaggcaa gggaataacg ccaagggaaa tgttcccaca
    16381 caagctgttc cagccgttcg gacaattcgg tcgcaaccat tgcccaagca atatgtttcc
    16441 ccgacgaccg tctatgttcc tagaacaatc cgtgtggtag agcgttcccc ggagccagag
    16501 ccggaaaccg tccaaaacta acagtaaaga ccatctgaaa aagagtttca gacggtcttt
    16561 tataatttcc ccttgacatg gatggtaaat tcatgtttat atcaattcaa ttatttagga
    16621 ggtatggaaa tggcttacgc aattgcattt gatttggata cgaccgtttt acaagaactt
    16681 taccctaata gtagctggca aaatgcttac ggtgatgtaa aaagaacctt ggtcggatta
    16741 ggttttaacc atcagcaagg cagtgtatat tttggaaact ctgagattac agccgttagc
    16801 tgtgtcttgg ccgcccaaaa actgagccag acctatgcat ggtttaagcc ggccgtttcg
    16861 gatattcgta tgttgcggat tgaggaaatg aacgatttgc agcccgcttt ataaagcgat
    16921 aggtatctta gaaaagggca gcgcggtgtc cccgccctgc cctttccctt tccttccagc
    16981 gttgccgagt aaaggctgcc tagtttctac taggcagcca aaactacttt gccttgcaag
    17041 cccgtttaac cgtactttcg ctaacattga aatgtgcggc cgtccgggaa atactggttt
    17101 gttcagactg ccgccatcgg cggatttctt cgggatcgac tttcactttc cgccccagtt
    17161 ttttcccttc ttccctagca ttgcggatac cggccatttg acgctctttg atgtaactgc
    17221 gttccaactc cgcaaatgcg ctgacaatag ccagcatcgc cgtacctaac ggactggcca
    17281 aatcgaagtt ttcccggagc gaggttacgg ccacgccctt tttctgcaaa aactgcaccg
    17341 tctccaaaac atcgatggta tttctgccca aacggtcaat ggctactacg actacggtat
    17401 ccccttggcg tacaaattca gccatcttac ggaactcagg ccggttaagg gcttttactg
    17461 cacccgaagc ggcctgatct tcataccacc gttcgatctg gtatttgtct tgaataacct
    17521 gtctttgtgc ggccacattc tgctctttgg tcgataccct caaatagccg ataatcataa
    17581 ttcctcaaac ggattaaaaa ctcttgcgcc caagtgtgaa aaatcctttt cattgcgcgt
    17641 taccaccatc atatgctcct gtccaacggt cgtcttcgat aagcacacgg tcaaactcaa
    17701 tgtctgcaat atctacctct tccggaggaa cggccaaggc atcagcgata ctctccccct
    17761 tacccaacga ttctgctcct aacgaaaggt catggggaca gccattcaaa aacggagaca
    17821 accgtacctt ataagccgga aaatcggtaa caaactttct cagtttttca tcaaccatcc
    17881 tttgaacaaa tggattgcct gtcgctgcct cgatgttgtg caaaatgtcg gccaaatctc
    17941 cgattatcgt caaattatcc agcgcagccg cataactctc aggccgatac ccgcgccggg
    18001 aataagacag ttccctgata atcagcgaaa tttccgtgat caaccgcatt ttttctttca
    18061 tttccgtatc cctgcttatc tgctgatgga ttttttggcg gaatactcca aagacttgac
    18121 aatcaatttg cccttattgc ttccgataga aacgttctgc cctatctcaa ccaatttggc
    18181 cagtctttct tcgttatgtg ttgcattgtg cctgacaagc ccgtttttgg ttttttgaaa
    18241 taccgcagcg gcatccttgt aaatgatttc tccggaataa gtttgaccag tctctgcttt
    18301 tttagcttga cctagtaaat agataccggc tttcatgcct gttacaggaa tttgttccac
    18361 cttagaccac aactcgtttg ctgactttgt ttctagggta acacccttgg agttagtaac
    18421 taccctttgc tgggaatact cttttttggc gaaaacactt tcaagatcca tactgccctt
    18481 ggcctgattg aagcattccc ggctgatttt tcctgagtcg aagtcgctct ggattttctc
    18541 ggccaacctg cccaatattt gctcttgcga ccccaaggaa agcattttga tgacatcatc
    18601 gaacttgtcg ataaattcac tgttcctgtc ttggttgata ccgacaattt ttaccgcatc
    18661 accaaactta tccctgattt gcttgagtcc gtcaggaaga ttggcctgaa tatcagccat
    18721 aatacctata ctggctcccc tgccgtattc gttaaaccgt ttgtaagtat tgtcggaagc
    18781 cctttcagcc ctcatgctga cggctacgat agtaacttca agattacgct ccagacactg
    18841 ctcaatcttg cgaaaagcag actgcggccg tgcaagctgt ccttcaaaaa gcagcttggt
    18901 ggtatcctgc atcatcatgt tttttactgt cgaagttttt cccgaacccg gaattcccgt
    18961 aacgaaaata acttctttct tctgtggctc gtcgcgtttc agtacctcgt caaactgggt
    19021 agaagataaa actgctgctg aattgtggat ggcattatta accgttgccc tgtcttcttt
    19081 gttctcaaaa gctggaaaca actctttaaa cgtatcagca caaacatatc taccgccttc
    19141 tgtatctgca tgttggctat ataaagacaa tatctcgtct gtttgttcgg tcgcaaaacg
    19201 gatggcctca tcctgaacgg aagacaaata ttcgttattg ccaaaatctc tcagattaat
    19261 atcgctagac agttttacca taacggatta ttgctcctta tttgccgccc acaacactct
    19321 taacttagct agaagctcag gagatgggcc ggcatacagg cgtttttcca cttctttgcg
    19381 gaaggcttcg tctgtcatca tcctgcggtt tttttcgcaa gcctctttta tcattttgat
    19441 agcattactt tcttggggct caactttatt cataataaaa tcctaatctt aattttatat
    19501 tactcattat cgcttcatcg ttttctttac ggaagcgtcc gatgctttga cggacaaccc
    19561 atttccttcc cgcttgatgg aaactttctg accaatttcc acctgttcgg acagactgga
    19621 gaatttcccc tccgacattc cctgaaccgc cttatggcgt atcagacctt ggtctgtctt
    19681 ctgaaaaact gatgcctcgt ctttatggac aatctctcca ctgtactctt tgccggattc
    19741 ggccggtttc gccgtgccaa gcatgtgaat accggctttc atccccttgg cttcagcctt
    19801 ctcaatatcc tgccatgtgt cgcctgattt gcgttgcagt gttgcccctt ctgaattggc
    19861 caccaccctt tcctgacgat aaatctcttc cacacgctga tagatctctt cccggtgttg
    19921 ctttaccttg tcaaaataat ttggaacagg atcttgggaa cgcctgtctg cctgaagctg
    19981 tatcacatcc cagacttgag caacctgctt caaacgttca ggagtaatct gctgataaaa
    20041 ctccatagca tctttggcac tcgcctgttt gttctgttgt ttattcaggc tgtcatacag
    20101 gatttccccg gggcgattgg ttacgcggat gcggtcgaat tccgcctcat gagttgcaac
    20161 tgtattttta aatccctcaa acgccttatt gtgaaaatct tcgtcaacga aacgcggttg
    20221 gatattctta gtaagatact gatcttcata ccgtttaaaa ataccggcca aactttcttc
    20281 cggagcagtc gcaacggcca acatttcgat tttcagtccc gcttctcgcg cggctttaat
    20341 gctcatgcct actgcttcag cattacggaa agttccttct tccaaaacgt tccgggagtt
    20401 ttccaatgcc gattttcgta ctcctacagc caacttgcct gcatcttctt gtgtctgctg
    20461 agacgaataa acgactccag gaggaactgg tattttttgc cgcataatgt cggcatcaac
    20521 atgtatatgc cctccctttg aagcgaactc ttcttttacg atgtcggcgg ccttactttt
    20581 tcccgctccc ggttgagccg ctaccaaggc gatctgcggc acatctaccg cctgtgtaat
    20641 ttccaaaaga gcagacatat attcggcaac aatttcggcg cgtcccgcgt cgcttaactt
    20701 ttggtaatca ctcatacaga acccccaaca atcgtatcat tgggatccaa aatccttgcg
    20761 atgacatctt cgtccgttgc tcttaagtta tggcggaata ctgtaatcgc tttaatacta
    20821 tccgaacaat attgaatgaa tgcttcattt ggatgagatt ttttccgctc ctcttctcta
    20881 gcctgaataa acggttgggc ggcattaccc aaaacctcta acgctacttc gtaattgata
    20941 cccggaatta cttctttcat tttttggttc cctccaaaaa ttatccacaa attttgttag
    21001 taagctgccc atatcgccag cctgccacac ataataaatt gaatcttcgt catcaaaccg
    21061 tttttgcctg ctttttaagc aacatggttc aaaacctatg tttgaataat aagccagttc
    21121 aaaataaata acaacgtttt tgacctatac ttccagacct taacgaaaca tagttcataa
    21181 agtacacttt ttgcacccac ttatctaggt tactcacctc ttactcaaga tttggccatt
    21241 gcccaccata ccccataatg gctttaattg actcctgtca agcccaaaga ctctctttca
    21301 tgtttataat tataggataa tttatcctac ttgtcaaata aattttagcc gctaaatttt
    21361 tactctcgct tatttattca ttataataaa ttaattgtta ttctgcttaa ctgtaaaaat
    21421 taactgtgag tgattggaga caccatgcca catgagcaaa tgattgatgc tttttcagca
    21481 atcattcaaa actccttaag cctatatgaa gagcaaactc cgctatacta ctacctgact
    21541 atctgcggtt cttgggttct gactgcactc ggcatcctct ccttggcgtc cgtattcttt
    21601 accatcaagc agaaaaaacc ataccgtttt atcgtcccga ttctgttttt atggctggga
    21661 tatgaaagca tccacatgcc gcgcccgaat cttgctgtat tctacggata caacttggcg
    21721 cagctttcct atccggcaaa cgaaaatccc aactcgttcc tacatctcgc agacggctca
    21781 aatccgattt gctgcttcat aggcgaagac gagtatttgc aagccaaacg gttaggtttc
    21841 aaagagtatg ccgatgcaaa agcaaaggct ttggttgatt tggttgaaat ggagaaaaaa
    21901 tccaaacaat ctgattgaat cttaaattca ataataacgc ggtagggggc tggtcaagcg
    21961 aaggttcagg ctctgactga aatttcgctt ggacaggggg tgtggggcgg agcgcagcga
    22021 agggctaccg cgtggcggcc gaagccgccc gacaaactcc agtttgtcgc tattccgttt
    22081 tcagtgcgta aatacctttc cccactctca gaaaaaaccg ctcgtcctgt aatacctgcc
    22141 tgactttggc ctgccagttc ctgttttctg cccgcgcagc ggcatacggt tccatttcgc
    22201 cataaatttc cgtcaagccc atattcctcc cgtctgccgc ctgcaatacc cgtcttacgg
    22261 cggctttcca tgtaacggcc gttaccgcct caaccctatt catcgcttta gccaaaaagt
    22321 acaaggccgt ctgaatctct tttttaaaga ccagcagttt ctcatgggca atccgtacca
    22381 gctttccgcc atattggcgg ctgtcgctga cgcagttgtg ctggattttg ataatctcat
    22441 ccaccagccg ccccgccgcc atccgttcaa ccatgctgct caaattgtag taacggccgt
    22501 ccttcctcat gttgcccatt agaatcccat aatgcccgcc ctgttcggtc gcatcatgga
    22561 tgttcatcat ggccagcagc atagcctctt gaaactgttc caagtccatt tggctcaaat
    22621 cccacggatt ggctgcatca ccccattggt tgccactgta tcgaatcatg ttccaataag
    22681 gcggatgcca ccaaattaaa ttggcctgtt ctcctaaaaa tgccgcaaaa tcctgagtca
    22741 gcaggttata tccctgatgc aaatccgttc ctttaaaacg aatgcccatt tcctccgcaa
    22801 catccgaact ggtaccgctg ccgatgctcg ggtcggcaaa cagtccttcc ggagcaagat
    22861 agctttccac aaagtcgcgg atgatatgcc ccgtgcaatt tccgcgataa ccgcttttac
    22921 cgtaatttcc gcgctgcgga taagaacaaa cagaatgcgt aaacatggtt cattcctcct
    22981 ttaaatcgtc ttcattttcg cgataatggc aacctaatac tgcatccaaa tcccgcatgg
    23041 tttccaccgg ctcgtcattg acataaaagt gtgtatccag cgcattgatt aaaaaacccg
    23101 tccgcaagaa cggccaccgg ttgcggatgg tatcctgcaa acgctccagc aaatgatgct
    23161 gccgaaggtt tccaaaatgc ggaacggttt catatgtccg tgccgtttcc caaatgtcct
    23221 gctcgaagtt gataaacgag tcgccgccga gttggcgcag cagattcatc caaccatcga
    23281 aactgtcgga cacatcatcc caataaagca taatttcctc cttatcactc aatccctgca
    23341 tcggccaaaa tcaaagccaa gatgccgctg cgccgtgctt tggcagtttt cagtttcaga
    23401 cactgcaaat catcgcaaac aatctgatac ccgcgcgtct ccagttcggc cgcttctgac
    23461 acttgcaggc tgtaatactg cccgccatcg aatgtgtatc ggcaaagcag tgtaggggca
    23521 tttcgtccgt cccacataac gttaatcgga ctgaatccca tcaactcccc attgctccaa
    23581 atattctgct cgacaaatac attcggatgc ccagttaatc tgttggtata gcttgccatc
    23641 tctgtctccc gtactttcct tggtgttaaa cggtttttga actgaaccag catcggcgtt
    23701 tccgccccgc tgtgatgacg ttaaaagaca aactgctttg agatacaagg ctgccgcagg
    23761 caccgcaggc tcggccttgt atctcaaagc agtttgtctt aggaatcacg caacagcggg
    23821 gcgggaatgc cggttgcgtc gaacggccaa aagccgtttg acaccaagga aaaccttctg
    23881 ccggccgcag ccggctcatt gcccctcacg gggaaggggt ggggttcgga gaggcggtgc
    23941 cgcagcacgg ccgatactta aaccaagagc ggtttgatgc cttagcatga aaccgctctt
    24001 tccgttcaat cctgctgact cttgattagc ctgaaactcc cagcaccttc aagataaccg
    24061 tttatagtct tgcgaatggt aacatattcc gtccccatca ttccgaatcc gcccttagtc
    24121 caaaaaaacg gctccctcgg caaaaaagga atatattcct cctcttgagg ctcgcccatg
    24181 ccaagactga tctcgggctg attcccatcc gtctcataat gcagcttttc gtcccctttg
    24241 aaaaaatcgg cgacgaccgc ttccgcctct tcaagagatt ggctgataaa taaagccgaa
    24301 ctaccaaagc cgatctcacc tgtctctaca aggtagaaag ttcgattttc agcggacaga
    24361 ctcattttct ccactcccga taaacgccta tgtcgttatg gataaaggtt ggatagatac
    24421 cgatcccacc taccctgaac cttcttgcca aagcggcaag ctcggaaagg gacgctcttt
    24481 cgatatgaaa gtcccccgca ttgccggaca aatgttggga gtttttggcc gccccctcta
    24541 acgagtcgtt atgtcggcgc gtgcggaatc cgctatgcaa aaccaactcc ggattcggtc
    24601 tgcccaaatc acgcaaatac tgctgctggg caaacagcag attgatcaaa ttgatgttga
    24661 tggcgtgcat ctgatggttg tccaccacat cccgcatcat ccaacacaga gccttatagc
    24721 cctcttggat atactggccg tcggcaaaga atttgatgtt tttccgttcc cgggaagcag
    24781 gccggtaaat ggacaatacc cggtttcgtt tccaaaaccg gctgtcatcc gccaaaacga
    24841 tttccggcaa cagtaaaagg ccgcctgatg aagcaagcgc ggccgctttg gccgacagct
    24901 ttaaaaaatc ccgacgattc aacgctccct ccctttcacg ttaccgctga ccataaaagc
    24961 ataccggccg tattcgttac ctccctcatt cgccgtttct ccggccgcaa gggatcccaa
    25021 aacctgctct aatcccattt cgaccgcagg tataaaatct tcatctgtcg ccgtaatggt
    25081 aatgctgagc gtcttggtat cggcatgcgt atctacccga tgattcagtc cgtactgctt
    25141 ctgaaatgcc ttgacggcgg cctgttcgga ctgattccgt tcggccgaca cgcccatttt
    25201 ttcctcttcg tgccgctgca acatttccaa ataatcgtca aacgccaagg gctggttctg
    25261 gctggttttc cggtaatcga cggcgatatt ttgcagcgtc tgccgcaggc ggccgtcaaa
    25321 ggccatgccg aaaacccgcc agaaccatcc ttccctggcc tgtttgtgat aagtcagaaa
    25381 aaaagccact ctgtataatt tgtccttatc cataacaccc cctatcggct gccgcgcgat
    25441 gcggttttcc gggttgttgc ccgctgctgc cgcttggtct gttcgacagg ccgatcctga
    25501 gacgcttctt cctgcaaacc gcgtgttttc agggtttgca attcgtttgc cggtcgggca
    25561 tgtatcgctt cgttgatttg cttggccaaa ccttcgcggc cgaacttgga tttctgagcc
    25621 aaatcgttga aatcggtaaa tgccttatgc tccgacgact gctctcccgg tgcaaacact
    25681 ggaaacactg cacggcagtt aaggattttt gccgcctcca acgctttttc tttgccggga
    25741 ttgttgccct gcttggcttc gacagccaaa tcatcgtctc cggcgaccaa aaactgagcc
    25801 tgagggtatt tcgcagacaa gactttggcc accaaaggca ggttgccgga attgaacgcg
    25861 gccacactgg ccaccgtatc gtcggttgct tctttaatcg aggcggccgt agcatagcct
    25921 tccgataaaa taatggtttt ggccttagcc agatcctgtt ggccgacaac gtgcatacaa
    25981 ccctcctgct cgctgtcttt ggcaaaacgt ttggtaccgt ccgcctgaat gtattgcatg
    26041 gagcgtaact ccccggctgc gttgtacacg ggaatgaaag ttttctgccc ctcgcggtcg
    26101 gtaaagatac ccggctgcgg cttgataccc ttggcattca gataaggcgt gcctgtttga
    26161 acgggcaggt agctttccat atagtatttg atacggccga cagcagcttc ctgagcctgt
    26221 tttttctgct cgctccgcat ttgcagcttc tgagcggcct cggcggccag cttggctttt
    26281 tcctccggag acaggctgta tccctttgcc ttccacttaa cctccgctcc cgtccggttg
    26341 tttttgatgt agcctgccgg gtggccgtcc aaatgaccaa cataaaagcc gctgcgctcg
    26401 ccttttttgt cgccgacggt ttcgattcgg tgtttttgtc cgtccataaa cggatgccca
    26461 ttagccacca cacaccccat ttcgcgcaac ttgtcggcaa actcttccac cggcgacatc
    26521 gcgggcatgc tttgagtctg ttgcttttcc ggcaaccatt ttgacgcggc cggtaaatta
    26581 gcgttactgc tttccataaa ccaagatttg ttggctttgt cccatttggc accaagggct
    26641 ttagcctctt ctttttcttc atacggaacg gcaagataaa cacgggatgc gttttcttct
    26701 gccggtgctt cttctgtttt ggcagcctga tactcttgct gtatggctgc gacctgacac
    26761 tcaactgctt cttcctgagg agtatcgtcc ggcacggcct cttctgccaa cgcttcctgc
    26821 ttcggctgca tccaccgtgc aaacagtgcg gtattcacgc cttcggggat ataccaagac
    26881 ttctgatcct tatcccattt cgcgcccaat gcccgcgctt cgtttttttc acgaaacggc
    26941 acatcaatta gcgttttcgc tatggccgga gtattgtccg ttaccgtttt tgcttggttg
    27001 gtggcactgt ccagccattt tctgaaaggc tcttgattga ccccgggagg gatgtaccac
    27061 gatttctcct ccttatccca tttcgcgccc agcatttttg cctgttccct gtcggcataa
    27121 ggaatttgaa cgtaaacgcg ctcatcggaa accacgcgga cgctttcctt gtcctgctcc
    27181 ggcagaaact ggcggatctg ttcttcaacc gtacgataag aggattgctc gatggcttcg
    27241 tccaaagcct ctttggccgc attcagattt tccataaatg actctccatt ctccggtttt
    27301 tgatgccgct gccaaaaagc gttctgaaac gtggcatcat ccatctccga aataaccaga
    27361 ctgaaacgct tcaagccctc gtgcattttc tcttcagcca tgaagttgat gatcgccgcc
    27421 acatcatttt tatcaaccgc attgataagg ttcttttctt ccgccaagct ggcttcatca
    27481 atattctgtc tatcagaaat actcactgcc gcctcgttct gctgttcgat ttcccgcacc
    27541 tgatcaaagg ccatgacata gccttggatt ttttcggcgt cagaagccgc acgcatgatt
    27601 tccatcggat cgtcctgcaa taccataatc cagcttgcta cataactggc atgttgcccg
    27661 ggattgaatg aaacgcccaa ttcctgactc agcatcaatg aagcgatttc ggcacgcaat
    27721 tcctcccgtg cgtaaccctc gctgccgaac gggtgggaaa ggtcgcggtt caaacggtca
    27781 gcatgccccg tccaatgccc caattcatgc agggctgttt cataatacgc tcccggactt
    27841 tcaaactgct cgcgcagcgg cagggtaata ctgtccctgc tcggagagta aaacgccctg
    27901 tcgccgtgcc ggtggtcgat gaccgcacca gaatttttca gaatgttttc tgcccgctca
    27961 tccggattcc aacttagcgg ccgcagcttc aacggcggca agccgtctat ctgttcgccg
    28021 ttaaatacca ccgcatgaaa gacctttgga cgctccaaac gaaccgtttc cttctccggt
    28081 ttgccgtcct ggccgataat ttcctgaccc ttttcatcgg ttttgggaac ttcctcggtg
    28141 aactgccaat attcgacaac agtacccttc tctccacggc gcacctgcgc cccgatggat
    28201 gatgcctgtt tataggttat ccagcgcgga tcctttctgc cttgtgcctg caaccacaga
    28261 ttgttaatgc cgcgataacg gcggccggta atggcattgt gcggcataaa atccggcaac
    28321 ccgccgccgt cccaaggttt ctgccacggc gcggttccct gccgcaactg ttcgatcagt
    28381 ttttccgcca cctgttcgta aaacggtttt ttattctgca tccctacgct ccttccgcca
    28441 cttcgcggga aaggtctgcc acggaatccc gcgcggtttc cgcatccatt gcggtctcct
    28501 caaacgcccc caaggcttct gcctctgccg gatcaaaatc cacttccatt cccgggttat
    28561 tcaaatccgc cactttttct gcgatcactt cgatgtcgat acttatacct tatcctttcg
    28621 tatgtttgga atagtccggc ttaccgccag catcggggta atactgcttg cactcggggt
    28681 aattgctgca tccccaaaaa tgcgttcctt tctgttttcc cggccgtctg accaagggat
    28741 gggtgcaggc catgcacttg aattttgacg acggctcggc ttttttcggt gccagtaccg
    28801 gccgaccggc cttgtcggaa aaagaaacct tgcatccgtc cttatagccg gtacagcccc
    28861 aaaaaaatcc tttcgatccg cgcatccgcc ttaaaggttt gccacattcg ggacacgggt
    28921 acttcttgac attgagactg atgccttccc gccctacccg ctccacctcg ccggatatgt
    28981 actccatcag actgttcaca aaacgttgcg cgtccaaacc gccttggaca atatgctgtt
    29041 gctgctcatg ccataaggct gtcatatcgg ggaacttcgc ctgatcgggc agcgcgtcat
    29101 aaagctgttg gccgatttcg gaactgacaa tgtttttgcc tttttccagc agaaaaccct
    29161 tgtccagcag attacgcaga atttcgtccc gagtggccgg ggtgccgatt ccgccgtgct
    29221 ctccttcctt acccttgtct ttctcaatca gcagttcgcg caatttcgga ttctgcacat
    29281 acttggctac ccgtgttaaa tcgttcaaca gcgtatccat cgtgtataac gggcgcggtt
    29341 tggtttcaaa ctgcgtctct tctgctgtca aacaccggcc gctgtttccc ggtttgagca
    29401 tctgcaaatc aacactcaaa tcctcgggat ctgtcgcggt ttccgcattg ccctgatcgt
    29461 tcttgtatag agccatccaa ccgggcttta cggttacgtt gctgcggcag acaaattttt
    29521 taccggcaat ttctgcttca atgtcggttt ggttatattc gtgatccggc caaaactggg
    29581 cgatataggc gcgggcaatc agcagataga cccgtttttc gtcttcggtc aggtcgtcca
    29641 tattggccgt tttgacggta ggaataatgg catggtgcgc cgaaacttta tccgggttga
    29701 acgccctgct tttgattttc gtgtcggccg atgcaatggc atttttaaag actgatgcgg
    29761 tttttccgac agcatccagc acgtctgccg catcatcgaa atgctcgtca ttgagatact
    29821 ggcagtcgct gcggtggtag gtaatcaaac ggtgtttgtc tttcaatgac tgtccgatgt
    29881 cttttacctg atcgggtttc aaaccgaatt tgcgcgaaca atccgcctgt agtttgagca
    29941 gattgtatgg cagcggcgga ctttgccgct tggcagaagt tttagccgct aaaattttta
    30001 ccggctgacc actggctgct tcggctatct gccgtgcaaa cagcggatcg gacaaacgtc
    30061 ccttatcgtc tatcggatct tgatccttgt ttggaacgta acgggcagta aaccggacac
    30121 catccacatc caaatcggca tacacatggt aataatgggt tttgacatgg gactggtttt
    30181 cccgatcgcg ccgcaccact aaacccaaaa tcggagtctg taccctgccg acggacaaca
    30241 ctccttgata accctgttcc gcagccttca gcgtataggc tcgggtcatg ttgtatccgt
    30301 ataactggtc tgccaccgat cgcgcttccg ccgattggga caatcccctg aatttggcgt
    30361 tgtcttctat tgccgataac gctctcttta cagatcgctc gttattgtcg ttaatcagta
    30421 cccgtttgac cggtttggca caattcaaaa actccaacag ttcgtcaacc aacaactgtc
    30481 cttcgtcatc gggatctccg gcgtgaacaa tctcggtaca ttccgccagc aaatcacgaa
    30541 taaccccgat ctgttctttt ttgtcggaca agggtttttt acgccaaggg atatgcgcga
    30601 tcggtaaatc ctcaaacttc cagttaccgt atttgctgtc gtaatcttcc ggatcgcaca
    30661 aagcaagcat atggccgtaa caccaagtga tgttgtctcc gttactgcaa cggaaatacc
    30721 cgttgcgcct ctcgatacca actcccaaag cggcggcaat ggctctggcc acttcgggtt
    30781 tttctgcaat ataaagtcgg gacataccta ctcctctatc catatcggtt ttacgacatc
    30841 acggatatgc tctctccgga ctatgccgta ataacgcgcg tcaaacgaat tggccaccgc
    30901 attaccaatc aaaatgtact catcgtcttt cagacggcct tccccttcaa ataccggtaa
    30961 gggctgccct agcccgtctt gccataaagg ccgggtgccg ccaagcagcc gtccgttaat
    31021 caacaaccct tcatcggtaa aacggtaatt gtcgccggga ataccggcaa caaacttcat
    31081 caacccggaa taattttccg gacaaaaacc cgctctgata taccccctcc ttcttgccat
    31141 tcggaaaact tcggtatcgg gagggcaaaa tgtaatgtag cttccgcgcc gtaccgcttc
    31201 gccggatatg cggtaaaccc ctttggccac actgcgggta tcgttgatac ggaaaccggc
    31261 tgcatataac agcccgtagc ctgttaccag caatgcggcg gtcagacaca tggcaagcat
    31321 ggcacggtca aacagccttc tcatagagtg tcaaacgcgc tgggcgtatt ggcagtcttt
    31381 ttctgagtcc ggtcggtatg gcgcggaggc gggactttgg agcgcgcctc aaataccgga
    31441 tctttgaaat acagggtctg ccgcccgtaa atcgccggat acccggcaac ataaatgacc
    31501 atatcgcctg cctgagtgat caaacccctt gcgtccttga ccggcccggg catacgcata
    31561 cattcgtcgg ctgtcagcaa cggccgctgg acttcctgcg tagtccggga aacctgcccc
    31621 aacatcgcac ttacccgttt gccgctggtt gtgatctgct ctttgacaac cgttgtgatg
    31681 ccggtcagct tggacaggtg ttccgccgtc tcgatccggt taggcggata agcgttctgc
    31741 acatggcagc tggacgtaat ggtttcatcc gggccatacc ccgtttcccg gcttttcagt
    31801 tggttcaagt cttggcagat caaataacat ttgatgccgt aaccggcgat aaacgccaag
    31861 gattcctgca tgatttccaa cttgcccaaa ctcgggaact cgtccagcat ggccagcatc
    31921 ttgtgtttgt agtggacttt cggcctcccg ttttcaaaat ccatcttgtc ggccgacaga
    31981 cggataatca tattgatcag aatccgaacc aaaggccgca accgcgcctt atcgttgggc
    32041 tgggtaatga tgtacagact gaccggatct tcgtcgttca tcagatcctt gattttgaaa
    32101 tcggaacgac tgacattggc ggcgaccaca ttgtcgcggt acaaagcgag gaaagatttg
    32161 gctgtggaaa gaaccgatcc ggcctcctct tccggtctgt ccagcatatc ctgtccggca
    32221 gaagaaatga ccggcagagg acggccgtct gcatggggat acgtcgccat ttccacccat
    32281 aaatcgccta ccggtttctc cggatcagac agcatgcggt caacgcttgg taaggtggca
    32341 accccgccat catccctagc tttgtaaagc gcatgcagaa tgacgccggt cagcaaagcg
    32401 aaagctgttt tgtcccagtg gctgttcagc cccttgccgt ccggatcgac aatcattgtg
    32461 gccagattct gaacatcgcc tatctcatac tccgtcccca ccctgatctc atccaaagga
    32521 ttccatgccg cgctgccttg cgcggaagcc ggttcaaagc ggattacctt gttgtgcgcg
    32581 tatttcttcc gccacccggc cgtcaatgcc cataattcgc ccttcaaatc ggtaatgaac
    32641 gtactgtgcg gccatgacag caaagtcggc aataccaaac ccaaaccctt accggatcgg
    32701 gttggggcat aagtcaggac atgctccggc ccggaatggc gcagataatg cagggtaccc
    32761 tttttatcga cccagccacc gacatataca ccgtcgctgt cctctccttt tttggccagc
    32821 agaccggccg cctcgatgtc tttcctgtcc gcccaacgcg ccgacccgtg cagagactca
    32881 tgcgccttat tggtattctt ccgcacaata ttccatacca acagccctat catgccgacg
    32941 gctaccacca tcaacatgat gctgaatgcc gtcataaact gctgctcgta ccgtccgtgc
    33001 catttaccgt accaaatcag aatgctccac ggcatataga acttgccgat gccgtatccc
    33061 aactgggaat ggtattggaa ctgatgggca aaatattgtg tggctgcccc cataccagcc
    33121 aacagcacca ataccagaca aataaagttt accaaacggc cgcttcctgt cgcttgggct
    33181 tggttctgat ggccgaccgc attgttgcgt ttgttcttgc tgcgaaacat gtttatcctt
    33241 tgaaatttaa cgatgccttc ccttaacagg tctgatctgc cccgtcggag taactttcac
    33301 ttcactgccc agtttcacgc tcgaaaggcg tctggacaca tagggtgtaa cggacatgac
    33361 cagtatttcc tcgacctctt tcggctggaa taaagccagt gcctgcccgt ctatgttgcg
    33421 gatgcccctg aacaacatca caccggaatc acttggatta tacttcctat gattcaatat
    33481 atcgaatttt ttgtcaattt tctcctgccg ctcggcaata tatttgtcga taggtgccaa
    33541 tgaatgagta agtctctcgt tttcttgtgc aggctcagga tttttcggaa tctctactac
    33601 cgcagcggat gaaattttga tttcagccaa cccttccatc ccccgcaaac tgccccgcct
    33661 gaatcccggc ggtttttttc ccggtttggc aaccaaaccg acaactgcct gcatctgcct
    33721 gatctcagct actcctcctg tccgatgtat tgtccgtcgc acagcctgat agtttgcgga
    33781 tccttgctgc tccaagtgac tagaaacatc acgcggcaat agcacttgag ttcgtccttg
    33841 gatgcgaacc agactgcgtt cgggcattcc tcgcagactg tcgcttcctg cggaagcaga
    33901 ctgccctctt cctgctccaa tgcctccaga gtcggagagc ttcgggattg ggtatcctga
    33961 tatgcctgca tggaagcctc gatttcggct tgcgtcatac ccatgccatc gatagcgtct
    34021 aggctttcct cctgcatatc ccgcgcctcc tcgttgctgc cctgcccgcc catctgcgcg
    34081 gccagttcca tcagcttgtc tgtatcgatc ggtacgttcc ctgacatatt cgtattcctt
    34141 cacatactgt tgataaagat ggttcagacg gccgtctgta aacgacacat ccaaaccctt
    34201 tgcggcggca attctggcca ccttatcctg aaaatgccgc gttccgccga tagtcaggac
    34261 ttttccgtat cgccctattg cgatttccaa cgcccttgat accgccttat cgtcaggaat
    34321 cgcggccagt ttgagcacac tgcccgtatc acggatggtc atgccgtcct cggcataaat
    34381 aaccgtcccc tttttcgtta ccgtatcaac cttgatcccg gcaaatccgc cactttcttt
    34441 gccgacaaag cggttgccct tgtaacctgc atccttcccc ctccttctca acgcatctaa
    34501 cgcccggaca tcgccttcgg cagcacggtg tttcagccaa tctgcccaag caaaaccctt
    34561 gttctgctca aaaaaaaccg cacgtcggcg ggcataatcg gttcttaccg atctgatttc
    34621 atccttcatt tgcctaaaca gcatccgata cagaatgcgc cttgcaatac cactgctgct
    34681 atgcctaatc aggctgcggc gaaaagcgta tttttctttg atgcggccga tctccatatc
    34741 ccggttttgt gccaaatgca ggaaaaaatc ctgccgcctg cttttctgaa gctctttttc
    34801 ctccttgtat tgccggaaca gatcgtccac gttggctttg ctcaacggtt tctcacggta
    34861 cgttttggat ggtttcgcct ttactcctgt tttctgatca aatgcaccaa gccttttttc
    34921 caacgccgac ttggaaaact ccctatctac cgaacttgcc ttgacatgat gctcgccgtc
    34981 gctgataaca aaaccgttac ccctcagctt gatttccaat ccgtgatcac tcagtacatg
    35041 gtacagttgc tcccatgtct tagcctcttt aatctgctca ccgcactctc tcctgatcca
    35101 tcctatcagg ctctcgatac ccgccacttc ctccatatcg atttttttat tgccgcgccc
    35161 gccacgctgc accctctgca aaccgtactc ttcttcaaac ttcctgcact cccgccctaa
    35221 aattttgaag tcgttatacg gctcgtggat ggtctttttt tctgggtgca ctttgttaat
    35281 cgctacatga aaatgcagat tgtcggtatc tctgtgaaca acagagatcc gctgatgttc
    35341 ggcatacccc aaagcctcaa tcatgctctg ctcaatacgg ctcaactgtt cgtccgtcaa
    35401 aaattccccg cccgcaaggg ataccatcag atgataggtt ttatccgccg cactgcgctt
    35461 attctgatcc tgaactgcca aagtctcctg aaccgcccac ctcaaatccg atgattcata
    35521 gttatgaatc tccacctttc ccactcggtt tgcgacacct tgagaaccgg ccagataggt
    35581 aactaagcct tcaaaactgc tcaggttggc tttacgcatc ggaatttttt tgcaatcatg
    35641 ttcagcctat ttttttagcc gctaaaattc tgcggctccg cacaatctcc aacatacttt
    35701 cctgcatttc tccaatttgc tccaacactc cgaagatcgc cttagccaac tgatcgtgat
    35761 cgaacatcat cagccgctcg tcgttggcca accacatctt gatcaagccg cccaatcggc
    35821 cttggtcggc attgattttg gccaaggctt taaccgcttc accgtcataa acggatgcag
    35881 gcttgtatcc caagccaagt aatttcaaat aagccgattg gctcaatccg acagatttag
    35941 ccaaactctc tatggttttc cattcttctt ccgaaacgta aacgctgatg tattttggcc
    36001 gttttcgtgc gcctgccatt cttggagtcc ttaatttgtg ccgcaaggca cacaacagcc
    36061 aacggtagtt ggccagcctc gcagagcaag ataatccgtt gagcctaccg gcgaataagg
    36121 attgtgatgt acatgtacat cacatatctt gccccaattc tggggttcac cccaattctg
    36181 gggttcaata cgccaaggat tatacgccaa ataattcaat atatcgaaca tattttgatg
    36241 acaaaccgcc taaaaatgct ttatcacaaa caaaaaatga tttactatat cgaattattg
    36301 tttttaaaac gattttgctt ggcagttttg gcaaggcttt gtcaaaaaat tggcaaagcc
    36361 ctatcgaaat gatccaaatg ggaaaaatag aaaaactggt tttggccgaa cttgccgcca
    36421 tgcagaaaaa aggctcctca gccggaaaca aggcaaggtt cttggccatg caggaagaca
    36481 ttaagatact gctggtggac aggaatatcc ccgttatcag tgtttggcgg attttgtcgg
    36541 aaaaaaaata tgtcccatat tcctatgaga catttaaccg ctactgcaag atgtatatct
    36601 tcaaatccga cactacggcc gctgaccaaa cagacaccgc ccagtccaca caggaaaaac
    36661 ctgattccat ccgcacggac aaagaaccgc cgtctcccgc ctccgaaccc attatcgcgg
    36721 gaggcaacac caaaagctct tcaggcttta ctttcaacaa caacccagac ggaaaggact
    36781 tattttaaat ggccattatc catatcagcc tccaagcaaa agggggcgtc ggtaaaagtt
    36841 tcttggcttt gatgctgtct atgttttacc gccatatcga aagaccggta aaaattttcg
    36901 ataccgatcc ggccaacgca accctgttcg gttttcccgc actggaggcg gaaaaattcg
    36961 agatcatgaa aaaggatgat attgacaaac ggttgtttga ccggcttttc tcacaaatcc
    37021 tgtctgcaac cgatgatgtc atcatcgatg ttggcgccaa tgcgtttatt ccgctttgtt
    37081 cgtatatcat ttccaacaat ctgattacca tgctgacgca ggaaggccat caggtcataa
    37141 tccacacttc cattgtcggc ggccagaacc tgaagcatac ggtcaacgga tttgatgccc
    37201 tatgctcgca atttccggaa tcaaccggtt tcgtcatttg gcttaatccg ttttgggggg
    37261 aaattgagtt tgaaggtaag tcgtttgaga aattgaaagt ctatgagaaa cacaaatccc
    37321 ggatacgttc catcattaag attccggaat gggacgcttc tctgcacggc cgcgaccttg
    37381 ccgccatgct ggaaaagcaa aaaacattcg acgaagcctt agcgtccgaa gatttcagca
    37441 tctttgaacg ccaacgtttg aacattatca aaaacgaaat gttccaaacg atagaaaccg
    37501 cccaaatcgt ctgagtacat catggatgaa aaagtaaaag ccatattagc caccattgtt
    37561 gaaaaaaacg gcatcgcctt atctcccaaa gacccgataa tggttgtatt cactgcctta
    37621 gaatatatgc tctccgaaca gcacgcagag caaaaggctc taaccgtctt attcaaagct
    37681 caggtagaag aggccacggg aaactggata cagtccgttc accaacagac tgaggaactt
    37741 gtcagccagc aactcaatac gaccaaagaa caggcaaagg aaatgtccaa attactgctt
    37801 catgagacag acaggctgct tgagcagaga attactgatg ccgtaaaaga ctttgaagga
    37861 aagctatacg gcatcaacga caacgccatg aatgcagtag aattgagaat aaaggaaact
    37921 cggaacacgg caattctcaa tctgacggcc gccctgattg tcttgttagc agtcattatc
    37981 ttggcgttca aatttacctt ctgacaaaaa acccggcaat gccgggtttt ttgtctccat
    38041 tctttacgcc aagagaaaaa tcagcgtact attttttgac ctagggacag gcttaatcca
    38101 gcttgccgtt tctgttgttc tttttctttg cgctgttttt catgcagatt gtgaaccctg
    38161 acagccgttt gaacatcatc aaattttttt gccagatccg gttcctccat ccttaacttc
    38221 ctagcagcca actcctgtat tttcggaaca cccagagcca tcccgtcgcg aatctcgcga
    38281 acatcttcca acctgtcatg cagacggttc agccttgatt gtgccactgc aatgtttgca
    38341 ctccacgtct gttttttgga cggcatgctc aacattcccg gccgggcgtt ttgcaatgcc
    38401 tgcaacttgg ccatttgccc ggaaatcaat tgctccaatt tttcttctat gcgctcaacc
    38461 tgttctgtct tttccaagac ataagtattt aacacaccgg cgaaagtttc ctcaataacc
    38521 tgcctgcttt caaacaactc ttcttgttcg gtttcagccg caacagcttt agctacaagc
    38581 cctttatctt cattcatggt gttatcctaa aattttagcg gctaaaattt catttagcgc
    38641 gttaaagttt ttttctctcc gatacccgcc ttttctactg ttgccgcttt gtccttgaca
    38701 ctgattgaga ccacttctcc gatcgacgga acggaagaaa atagagaaca cgggtatttg
    38761 actatagact gtcgtgtttt ctgataaaca tagctgtcat tttgaaaaac gatttgccct
    38821 tcgtacctga cagaatcctg aggagatttg gccgtatgca gcaaataaat tcccggcttc
    38881 atattggcag gagcaggctt aaccgcctgc gtaatccatt tgccgtcttt ttctgtctcg
    38941 aacaaatgct gtttgttcaa taccagcact ctttgttttt ctgcttccat tctgctaaaa
    39001 attttcaaaa aagatgtgaa taattctaac agaacagtc
//
LOCUS       DAXFWM010000012        38911 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000012 DAXFWM010000000
VERSION     DAXFWM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38911
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.012"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            183..2189
                     /locus_tag="ACFI8B_000588"
     CDS             183..2189
                     /locus_tag="ACFI8B_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC3833876.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2312..3403)
                     /gene="mltB"
                     /locus_tag="ACFI8B_000589"
     CDS             complement(2312..3403)
                     /gene="mltB"
                     /locus_tag="ACFI8B_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC3833877.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3551..4978)
                     /locus_tag="ACFI8B_000590"
     CDS             complement(3551..4978)
                     /locus_tag="ACFI8B_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC3833878.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5163..8867
                     /gene="mfd"
                     /locus_tag="ACFI8B_000591"
     CDS             5163..8867
                     /gene="mfd"
                     /locus_tag="ACFI8B_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC3833879.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDGIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDDPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQKINAIHEELVDRFGLTEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9093..9395
                     /locus_tag="ACFI8B_000592"
     CDS             9093..9395
                     /locus_tag="ACFI8B_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFC3833880.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9557..9940
                     /gene="panD"
                     /locus_tag="ACFI8B_000593"
     CDS             9557..9940
                     /gene="panD"
                     /locus_tag="ACFI8B_000593"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC3833881.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9962..10804
                     /gene="kdsA"
                     /locus_tag="ACFI8B_000594"
     CDS             9962..10804
                     /gene="kdsA"
                     /locus_tag="ACFI8B_000594"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC3833882.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10819..11259
                     /locus_tag="ACFI8B_000595"
     CDS             10819..11259
                     /locus_tag="ACFI8B_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC3833883.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11306..12592
                     /gene="eno"
                     /locus_tag="ACFI8B_000596"
     CDS             11306..12592
                     /gene="eno"
                     /locus_tag="ACFI8B_000596"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC3833884.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEVAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12760..13038
                     /gene="ftsB"
                     /locus_tag="ACFI8B_000597"
     CDS             12760..13038
                     /gene="ftsB"
                     /locus_tag="ACFI8B_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC3833885.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13079..13369)
                     /gene="yfaE"
                     /locus_tag="ACFI8B_000598"
     CDS             complement(13079..13369)
                     /gene="yfaE"
                     /locus_tag="ACFI8B_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC3833886.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13490..14623)
                     /gene="nrdB"
                     /locus_tag="ACFI8B_000599"
     CDS             complement(13490..14623)
                     /gene="nrdB"
                     /locus_tag="ACFI8B_000599"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC3833887.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(14936..17215)
                     /gene="nrdA"
                     /locus_tag="ACFI8B_000600"
     CDS             complement(14936..17215)
                     /gene="nrdA"
                     /locus_tag="ACFI8B_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC3833888.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17746..18057
                     /locus_tag="ACFI8B_000601"
     CDS             17746..18057
                     /locus_tag="ACFI8B_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC3833889.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(18107..18217)
                     /locus_tag="ACFI8B_000602"
                     /pseudo
     CDS             complement(18107..18217)
                     /locus_tag="ACFI8B_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18320..19087)
                     /locus_tag="ACFI8B_000603"
     CDS             complement(18320..19087)
                     /locus_tag="ACFI8B_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC3833890.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(19175..20002)
                     /gene="mutM"
                     /locus_tag="ACFI8B_000604"
     CDS             complement(19175..20002)
                     /gene="mutM"
                     /locus_tag="ACFI8B_000604"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC3833891.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(20055..20720)
                     /locus_tag="ACFI8B_000605"
     CDS             complement(20055..20720)
                     /locus_tag="ACFI8B_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC3833892.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            20899..22875
                     /locus_tag="ACFI8B_000606"
     CDS             20899..22875
                     /locus_tag="ACFI8B_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC3833893.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            22943..23635
                     /locus_tag="ACFI8B_000607"
     CDS             22943..23635
                     /locus_tag="ACFI8B_000607"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC3833894.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            24109..25479
                     /locus_tag="ACFI8B_000608"
     CDS             24109..25479
                     /locus_tag="ACFI8B_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3833895.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            25712..26368
                     /gene="cmk"
                     /locus_tag="ACFI8B_000609"
     CDS             25712..26368
                     /gene="cmk"
                     /locus_tag="ACFI8B_000609"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC3833896.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQSDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            26521..28206
                     /gene="rpsA"
                     /locus_tag="ACFI8B_000610"
     CDS             26521..28206
                     /gene="rpsA"
                     /locus_tag="ACFI8B_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC3833897.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            28217..28531
                     /locus_tag="ACFI8B_000611"
     CDS             28217..28531
                     /locus_tag="ACFI8B_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC3833898.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(28727..29134)
                     /locus_tag="ACFI8B_000612"
     CDS             complement(28727..29134)
                     /locus_tag="ACFI8B_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC3833899.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            29284..30394
                     /locus_tag="ACFI8B_000613"
                     /pseudo
     CDS             29284..30394
                     /locus_tag="ACFI8B_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            30403..31230
                     /gene="fghA"
                     /locus_tag="ACFI8B_000614"
     CDS             30403..31230
                     /gene="fghA"
                     /locus_tag="ACFI8B_000614"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC3833900.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNPENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            31760..32911
                     /gene="zapE"
                     /locus_tag="ACFI8B_000615"
     CDS             31760..32911
                     /gene="zapE"
                     /locus_tag="ACFI8B_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC3833901.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            32970..33395
                     /gene="ndk"
                     /locus_tag="ACFI8B_000616"
     CDS             32970..33395
                     /gene="ndk"
                     /locus_tag="ACFI8B_000616"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC3833902.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            33539..34633
                     /gene="rlmN"
                     /locus_tag="ACFI8B_000617"
     CDS             33539..34633
                     /gene="rlmN"
                     /locus_tag="ACFI8B_000617"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC3833903.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            34636..35397
                     /gene="pilW"
                     /locus_tag="ACFI8B_000618"
     CDS             34636..35397
                     /gene="pilW"
                     /locus_tag="ACFI8B_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC3833904.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            35413..36678
                     /gene="ispG"
                     /locus_tag="ACFI8B_000619"
                     /gene_synonym="gcpE"
     CDS             35413..36678
                     /gene="ispG"
                     /locus_tag="ACFI8B_000619"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC3833905.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKCNKGKVIPIQSL"
     gene            complement(36732..37346)
                     /gene="clpP"
                     /locus_tag="ACFI8B_000620"
     CDS             complement(36732..37346)
                     /gene="clpP"
                     /locus_tag="ACFI8B_000620"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC3833906.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(37442..38752)
                     /gene="tig"
                     /locus_tag="ACFI8B_000621"
     CDS             complement(37442..38752)
                     /gene="tig"
                     /locus_tag="ACFI8B_000621"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC3833907.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         9839 a        10740 c         9273 g         9059 t
ORIGIN      
        1 ccgaaattgt tataatcgaa aaaattaaaa atcaatacgg attattctat cagattgatt
       61 tatcgatatt tattatatca ttaatatagt ttgatttcaa accggcggaa aatgcgccgc
      121 ccgccgcaga tgcgccctcc ccgccatcgg ggcgtttaac gccggattgc cgatgcggta
      181 caatggcaga tatgaagaaa attacccctc aaaacctgcg tcccctgctt tcggaaagct
      241 tgggacatac cgattttgtc aacgtcctca acgcattgat taaatttttg cgccgtggcg
      301 gcaaaaaatg tgcgggggag cgtttcgacc tgattatcga cacattcaaa caagacaggg
      361 aattactgtc ccgcttcagc cggtgttttt acatctggct cgcgcaaata cacatttatc
      421 cggcactcat caaactcggc attttctcgc gccacagctt tgcccgggaa atgggcatac
      481 gcatatacga acgcttcaac ccgtcatata aagattttgc caacttgggc gaagtcttcc
      541 tttatctttt ccattccgaa aacgacgaca aatggctgca aacgctcaat atccgccaat
      601 ggctggtttt atacgaactc atccgtagcc acgccgagcc gtccaaattg cagacggcgg
      661 gcatccgcct tgccgatgcg cgtttgcgcg ccatcgaaat gctgtctgtc tggacggcat
      721 ccgaagccat cgaacccgat ctcatccgta tcgccccgcg cctgctggaa gccgattctt
      781 ccttcgtcgc cctccaacgc gaaaccgcca aactggtcga acactaccgc aacggcaccg
      841 cgccttacga caccgcccac ctcgaagtga tgttcgacca atgtttcagc cagattgact
      901 atttgcgtcg caaagggacg ggcgccggct ccggttcgtc ggtcaaagtc gcccacctgc
      961 tcgaacgact ccggcagacc gtaggccgtc tgaagctgct cacagacata caaaccggtg
     1021 ccggcaacag caaccgcctg accatcgccc tgatgaactc cctcatctac gcggcggtcg
     1081 aacaatacag cacccgccac ctgcgccgca gcagcatccg tatgctcgcc cgcagcatta
     1141 ccgaaaacaa aagccaccac ggcgaacact acatcacccg caaccgcaaa gaatatttca
     1201 aaatgttcta ctcggcagca ggcggcggcg gcatcatcgc cctgatggcg ctgctcaaaa
     1261 tccgcatcgg cacactcgac ctcagtccct tcctcgcttc cttgtcggct gggttcaact
     1321 acggcatcgg ctttatgatc atccatatgc tgcactgcac cgtcgccacc aagcagcccg
     1381 cgatgactgc cgccagcttt gccgaacagg tcgatctcaa cgaaggcggc aaagcggtgg
     1441 acaacaaact ctccaagctc ctcatcgacg tatgccgctc ccaaagcgtc gccgtcttcg
     1501 gcaacgtttc catcgccatc cttttggcgt gcgccatatc gttcggctat gcccatctgt
     1561 accggctgcc catactcgat gcccacaccg ccgcctacca gttcaaatcc atagacatca
     1621 tcaatcatcc taccctatgg tatgccgcta ttgcaggctt gtggctgttc tgctccggca
     1681 tcatcgcagg ttttttcgac aaccgcgccg actacctcaa cctgcgccaa cgcctgccct
     1741 tcaatccctt gctgcgtaaa atcatgcgcc ccgggccccg ccgcgtcctc gccgcctaca
     1801 tccacaaaca ctacggctcg ctggtcggca acttcatctt cggaatgctc ttgggtatga
     1861 ccggctattt cggacacctc ctcgggctgc cgctggacat ccgccacgtc gccttttcct
     1921 ccgccaacct cggctatgcc gccgtcagcg gcaacgtcgg ctttggtaca ttcgtactcg
     1981 gcattttcag cgtcctcgcc atcggcttgg tcaacctctg cgtcagcttc agcctcgccc
     2041 tcttcgtcgc cctgcgctcg cgcggcacaa aaatcggcag catccgcaac ctgatcaaaa
     2101 gtttttggaa tcagattaaa agcaatcctt gcatactttt cctcccgccc gccaaaggac
     2161 agggacatcc tccttcggac aagccttgac cggcaatgcc gtctgaaacg ggattcgccc
     2221 cgaataccgc cctgatgcgg gaaatcccca taaaaggatg caaaaatgcc gtctgaaccg
     2281 aaacgtggtt cagacagcat tttaaaaaac attacaatcc cgggccgccg agcgaattgg
     2341 caatgtccct gaccgccgtt acatacatcc ggctgtggtt gtactgccat accgtataaa
     2401 aattgttcaa gcccaaataa tattcaaaca cgccgggtgc ggtttccagt ttgaacaaaa
     2461 ccgccttttc atcatctgcg agcgtttccc cggggatgat gccgtacgcc ttcaaatccg
     2521 ccaccgtccg cgtcagggcg gttttttcgc caatgattgc ctgaacatcc gcacccggcg
     2581 ccaacgtcgc cgacaccaac attttaccgc ccgtgcgcca accgtgctgc ttcatataat
     2641 tggcaaccga tgccgcgaca tcaccgacgt tgccccatat atcccgatgt ccgtccccgt
     2701 cataatccac cgcccatttc cggtagctcg aaggcataaa ttgcggcata cccattgcgc
     2761 ccgcatagct gcccttaaag gcgaaaacat cgccgccttc ttcttttgcc agctttaaaa
     2821 gctcgaccaa ttctttttgg aaaaacccgg cgcggcgggg ataatcaaag cctaaggtcg
     2881 ccaatgcgtc cgccacacgg aagctgcccg tatttttgcc gtaattcgtt tcaatcccga
     2941 taatcgccac gataagctcg gcaggcacgc cgtatttttg cgccacatca tcgataaccg
     3001 cgcggttttc cgcataaaac ctgcgcgcgc cgtgaaattt cgccctgccc gaatttcccg
     3061 tgcggaacac ataccacgga cgcgatgtgg agggtcggtg cataatcttg acgatgtccg
     3121 ccttgtaagc cgctttgtca aaaaaatcct gccattccgc ctgggaaaaa tcccctttcc
     3181 cgacttcatc gtccacaaaa cggcggacat ttgcattggc ggcaaacccg ctgtcggata
     3241 ccggtacggc tgccgcgtca aacgcggggc ggctttcttt tttcatttca tccgcgcggg
     3301 gggcttgggc ttcatttgcc cggggtgtgc gggcctccat cgccgtacag gcagacaaag
     3361 ccgccaaaca aattgccagc ggcagtattt ttctcttttc cataaacaca ttccgaacaa
     3421 atagggtaag tgggaaagcg gcacaagggt gaggcgcgaa atgcggtatc cgccgcaatc
     3481 ggcggttttg cagaatgccg cgcgttgcct tttgcgccgc ccgaaatctg cctgccgccg
     3541 tatttgcccg ctatccgcaa tatcggcagt ccaatatatc tttgcggatg tcgttcagca
     3601 ggaaggctgt ggtgtcttcg tgttcctcct gtacgaagac aaagagtcgg gcgatgattt
     3661 tgccgatgaa gactttttgc agggcgcagg cgtcggtcag ggtgtgttcc tgcaggaagc
     3721 tgcggatgtc ttcgggcaaa gcgtagtcgc gggcaacggc ggcaaagcgg acatcggttt
     3781 gcaggcggtc gagcagggtt tggtttttgt ccaacttaat gcctgcttct ttttcttcgg
     3841 cggtggcgcg gacgaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa
     3901 aaatcgtgaa ggggcggata tcgatggcga tattgccggc tggatgtccg cgttcaaaac
     3961 ccttcgcgcc caagagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga
     4021 gggttttgac gatgttcgcc tccatcaatt gatgggcgac gggcgcgacg ggcgaaacgg
     4081 aatggcagac gtagcggtaa aggatttcgg aaacctgatg gcggcgttgg atttcgcggc
     4141 gttcgtaatc gacgaatctg atatcgttgc ggacatatcg gttcaggttg tcaaggatgt
     4201 attccataat gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt
     4261 ggaacgcgcg caaaccggca gcgtcgcccc gggagagttt catcacggcg gttgcaggca
     4321 tttcggcatc gatgcggttg acggcgtaac ggacggcgcg caggccttcg gatgcgaggg
     4381 tttcgcagcg gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc
     4441 cgtttttgcg ctctttggcg gcaacgagga ggaagtcgct ttgcgaattg ccctgccagt
     4501 atttcgcggc gttgacgtaa atggtttgtt cgtcggtata ttcgtagcag gactgcattt
     4561 cgcgtgcaat cgccgcgccg gaggtttcgg gttcggtaac gcccaaaccg ccgccttcgc
     4621 ctttgaaaat catgtccaaa ccttgtgcga cttgcgcttc gccgccgaac tcttgcagag
     4681 gctgcaacac cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc
     4741 ccgcaatcct taggacttct tggatttcaa actgactgcc cttgcgcccg ccgtgttttt
     4801 tgtcgaggaa gggcaacagc aaacccgcct gcttcaaggc aagccatttg ccttcgggca
     4861 ggtatcgcat caggtcgatg ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct
     4921 ttaaaaaagc agccgtgtcc atggttgacg gctgcgcgct cggttcggtg tgtatcatcg
     4981 gcttcctctg tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag
     5041 ttaattttct aaactttacc acaaagtgca ccgggcaaca atccgccggc ctttcagacg
     5101 gcatcgcgca ccctcacgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca
     5161 ctatgacctg ccccatcccc aaaccccgtg aaaaatcccg ttggttcaat ctttcgcaag
     5221 gctcgctgcc cttggctttg gcgcgttatt tgccgcacaa gcggctcaaa gccgtgctga
     5281 cccaagatgc ggaacaggcg ttgcgccttc agacggcatg gcggtttttc cgtccgcacg
     5341 acacggcggt gttcctgccg gactgggaaa cgctgcctta cgagcgtttt tctccgcatc
     5401 aggatttggt gtcggagcgg ctgtcggcgt tgtggcagat taaaagcggc gcggcggacg
     5461 tgttgttcgt accggttgcc acggcgatgc agaagctgcc gcccgtgccg tttttggcgg
     5521 ggcgcacgtt ttggctgaaa acggggcaga ctttggatat aggccgcctg aaaaccgatt
     5581 tggtggatgc gggctacaat catgtttccc acgttgtcgc ggcgggcgag tttgccgtgc
     5641 gcggcggcat tgtcgatttg ttcccgatgg gcagcgaaac gccgtaccgc atcgatttgt
     5701 ttgacgatga aatcgacggc attaaaacct tcgataccga cacgcagcgc accatttccc
     5761 ccgtttccga aatccgcctg ctgccggcgc acgagttccc caccgacagc gaggcgcaaa
     5821 aaatcttccg cagccgcttc cgcgaggaag tcgacggtaa tccgaacgat gccgccgtgt
     5881 acaaagccgt cagcaacggc cacttcggcg cgggcgtgga atattatctg ccgctgtttt
     5941 ttgaaaacga gctggaaacg ctgtttgact atatcggcga agatgcgctg tttgtctctt
     6001 tgggcgatgt tcatgccgag gcaaaccgct tttggaacga cgttaaatca cgttacgcca
     6061 tggcgcaggg cgacgaaacc tatccgcctt tgcttccaca gcatttgtat ctctctgccg
     6121 atgtgttcgc aggccgtctg aaaaactacg gacaagttct gcctgatgtt tccggcaagg
     6181 cacactccct gcccgacctt gccgtcaacc gccaatccga cgatcctttg caggcattga
     6241 aggattttca gacggccttt gacgggcgga ttttgctgtg cgccgaaagt ttgggacggc
     6301 gcgaaaccat gctcggtttc ttgcagcaaa acggtttgaa agccaaacct gtgtccgact
     6361 ggcagggctt cttgtcggcg cacgagccgc tgatgattac ggtcgcgccg ttggcttacg
     6421 gattcaaact gggcggactg caatcttcaa gccaacagca aactgttccc gcctccgagg
     6481 gagaaggcaa agcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc
     6541 ccagccctct cccgcaggag agggaacaaa gtgccgccgc cgtttcagac ggcctgaaag
     6601 cagccgccgt ttcaaccgaa agcagcctgt atctcgtcgc aagtgatctg cacgggcaaa
     6661 cccgacagca atctgccccc tcccccgtgg gggagggttg gggagagggc aaagcggttg
     6721 ccgctcaaag tgccatcgcc gtcatcaccg aatccgatct ttaccaatac gtcgcccgtt
     6781 cgcgcgtcca caaccgccgt aagaaacacg ccgccgtttc agacggcctg ttgcgcgacc
     6841 ttgccgaaat caatatcggc gatcccgtcg tgcacgaaga acacggcatc gggcggtata
     6901 cgggcttggt aacgatggat ttaggcggcg aaaccaacga aatgatgctg ctcgaatacg
     6961 cgggcgaagc gcagctttat gtgcctgttt cgcaactgca tttaatcagc cgctactccg
     7021 gtcaggcgca tgaaagcgtc gccctgcaca agctcggcag cggcgcgtgg aacaaggcga
     7081 agcgcaaagc cgccgaaaaa gcgcgcgata ccgccgccga gttgctcaac ctctacgccc
     7141 aacgcgccgc ccaatcggga cacaagtttg aaatcaacga gtcggactat caggcgtttg
     7201 ccgacggctt cggctacgag gaaaccgaag accaggccgc cgccatcgcc gccgtgatta
     7261 aagatttgac gcaggcgaag ccgatggacc gacttgtgtg cggcgatgtc ggtttcggca
     7321 aaaccgaagt cgccctgcgc gccgcgtttg tggcggtgat gggcggcaaa caggtcgccg
     7381 tactcgcccc gaccacgctt ctggtcgagc agcacgcgca aaacttcgcc gaccgtttcg
     7441 ccgatttccc cgtaaaagtc gccagccttt cacgtttcaa caacagcaaa gccaccaaag
     7501 ccacgctcga aggcatggcg gacggtactg tcgatatcgt tatcggtacg cacaaattgg
     7561 tgcaggacga cataaaattc aaaaacttag gtttattgat tatcgatgaa gaacaccgct
     7621 tcggcgtgcg acagaaagag cagctcaaac gcctgcgcgc caatgtcgac atcctaacca
     7681 tgaccgccac gccgattccg cgcaccctca gcatggcgct cgaaggtctg cgcgacttct
     7741 cgctgattac cactgcaccg agccgccgcc tcgccgtgaa aacctttgtc aaaccgttca
     7801 gcgaaggcag cgtgcgcgaa gccgtgttgc gcgaactcaa acgcggagga caggtatttt
     7861 tcctgcacaa tgaagtagat acgattgaga atatgcgcga gcggctggaa accctgctgc
     7921 ccgaagcccg tatcggcgtg gcgcacggac aactgcgcga gcgcgagctg gaacaagtca
     7981 tgcgcgactt tttgcagcaa agattcaatg tgttgctctg ttccaccatc atcgaaaccg
     8041 gcatcgacat ccccaacgcc aacaccatca tcatcaaccg cgccgacaaa ttcgggctgg
     8101 cccaactgca ccagcttcgc ggacgcgtcg gccgcagcca ccaccaagcc tacgcctacc
     8161 tgctcacgcc cgaatacatt accaaagacg cagaaaaacg cctcgacgcc atcgcggcgg
     8221 cagacgaact cggcgcaggt ttcaccctcg ccatgcagga tttggaaatc cgaggcgcag
     8281 gcgaaatcct cggcgaagga caatccggcg aaatgatgca ggtcggcttc acgctctaca
     8341 ccgaaatgct caaacaggcc gtgcgcgacc tcaaaaaagg ccgccaaccc gacctcgacg
     8401 caccgttggg cattaccacc gaaatcaaac tgcacagccc ggcattactg cctgaagact
     8461 actgccccga catccacgag cgcctcgtcc tctacaaacg cctcgccgta tgcgaaaccg
     8521 tgcagaaaat caacgccata cacgaagaac tcgtcgaccg cttcggcctg accgaacaac
     8581 ccgtcaaaac cctcattgaa agccaccacc tgcggcttgc ggcaaaagaa ctgggtattg
     8641 atgccatcga tgccacaagt gaagccgtaa ccgttacctt cggcaaacac cactgtatcg
     8701 acccgacggg gattatcctg ctgattcaga cggataaaaa ataccggctg gcaggggcag
     8761 acaaattgag gtttgcagcg gaaatggaaa acatcgaggt caggatcaat accgtcaaaa
     8821 ccgtactaaa aaccctgcaa ggcaaacgcc tgcccaaagg aaattgatgt aacgatgccg
     8881 tctgaagaac accgttcaga cggcatacaa tcccccaatc ctcaccaaaa ggaacaatca
     8941 tgtcgatttt tcacccctat ttccaacaac tgtccaccga aggttttgac ggagaagaag
     9001 atgtcctctg ggaagacgag ctgaccgtca aaggcaatct tgtcgaggta ttcctgtagg
     9061 cagaaaatga aaacagcctg ccaaccgaaa cattggatgc atttgccgcc ttcctgtccg
     9121 aattggaaaa agccgacgat gctgcacgcg ccgcactgac ggaatatttg aaacgggaca
     9181 gccgctatat cgactttcac accgaagata cccaaacaag ccgtgatgcg gcaaaatttg
     9241 tccgcactat gcagttgatc tatatcagct tatgggcaaa aaatccggct tttgccgtta
     9301 tggattatat gcccgccaat atggaaagcg acgaaatcct tgccgtcaaa ctgcatttgg
     9361 acggcagcat tttctcaatc gattgggaga gttgagccgg attgccaatc ggcaaaaatg
     9421 aaatgccgtc tgaaaacagc ttccctgccc gaagccttgc cattatgaat ttgaaggaaa
     9481 ctccactata atacggcatt cagattttca gacggcatca cgcccgtcag accgcacaca
     9541 aaccaaaagg aaatacatgt tccgtactat acttggcgga aaaatccacc gcgccaccgt
     9601 aaccgaagcc gatttaaact acgtcggcag cattaccgtc gatcaagacc tgttagacgc
     9661 ggcaggcatc tgccccaacg aaaaagtcgc catcgtcaac aacaacaacg gcgaacgttt
     9721 tgaaacctat accattgcag ggaaacgcgg cagcggcgtg atttgcctga acggtgctgc
     9781 agccaggctg gtacagaaag gcgacatcgt catcattatg tcttatatcc aactttccga
     9841 accggaaatc gccgcacacg aacccaaagt cgtcttagtg gacggaaaca ataaaatccg
     9901 cgacatcatc tcctacgagc cgccgcacac cgtactgtaa tcccgcaaac ggacatcgat
     9961 tatggatatt aaaatcaacg acatcaccct cggcaacaat tcgcctttcg tcctattcgg
    10021 cggcatcaac gttttagaag atttggattc caccctccaa acctgtgcgc attacgtcga
    10081 agttacccgc aaactgggca tcccctatat ctttaaagcc tctttcgaca aggcaaaccg
    10141 ctcgtctatc cattcctatc gcggcgtagg cttggaagaa ggcttaaaga tttttgaaaa
    10201 agtcaaagca gagttcggca tccccgtcat taccgacgta cacgaacccc atcaatgcca
    10261 acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgcc tttcttgcgc ggcagaccga
    10321 tttggtggcc gcaatggcgg aaacgggcaa tgttatcaac atcaaaaaac cccagttcct
    10381 cagcccttcg caaatgaaaa acatcgtgga aaaattccgc gaagccggca acgggaagct
    10441 gattttatgc gaacgcggca gcagcttcgg ctacgacaac ctcgttgtcg atatgctcgg
    10501 tttcggcgtg atgaaacaaa cctgcggcaa cctgccggtt attttcgacg ttacccattc
    10561 cctgcaaacc cgcgatgccg gttctgccgc atccggcggt cgtcgcgcac aggctttgga
    10621 tttggcactt gcaggcatgg caacccgcct tgccggcctg ttcctcgaat cgcaccccga
    10681 tccgaaactg gcaaaatgcg acggccccag cgcgctgccg ctacaccttt tagaaaattt
    10741 tttaatccgc atcaaagcat tggacgattt aatcaaatca caaccgattt taacaatcga
    10801 gtaacacggt ttcgccttat gatgcagact ttccgaaaaa tcagcctgta tgccgcaacc
    10861 ttgtggctcg gtatgcagat tatggcaggt tatatcgccg caccggtgct gttcaaaatg
    10921 ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt tcgacatcct ctcttggagc
    10981 gggcttgccg tttggggcac ggtactggct gccgcctttg ccgccctaac ccggcggcaa
    11041 accgccctgc tgcttttttt attgtccgcc cttgccgcca accaattttt ggttacaccc
    11101 gttatcgagg cactgaaata cgggcatgaa aattggctgt tgtcggttgc aggcggatcc
    11161 ttcggaatgt ggcacggtat ttccagcatg actttcatgg caaccgccct actttcagca
    11221 gttttaagtt ggcggctttc cggcaaagag gccgtctgaa gccctcccat tttttacctc
    11281 ccttcacttc acttggagaa cattcatgag cgcaatcgtt gatattttcg cccgcgaaat
    11341 tttggactcg cgcggcaacc ccacagtcga gtgtgatgta ttgctcgaat ccggcgtaat
    11401 gggacgtgcg gccgtaccga gcggcgcatc caccggtcag aaagaagctt tggaacttcg
    11461 cgacggcgac aaatcccgct attccggcaa aggcgtattg aaggcggtcg aacacgtcaa
    11521 caaccaaatc gcccaagccc tcatcggtat cgatgccaac gagcaatctt atatcgacca
    11581 aatcatgatc gaattggacg gtactgaaaa caaaggcaat ttgggtgcga atgcgacttt
    11641 ggcggtctct atggcggttg cacgcgccgc tgccgaagac tcaggcctgc cgctttaccg
    11701 ctacttgggg ggcgcaggtc cgatgtccct gcccgtaccg atgatgaacg tcatcaacgg
    11761 cggcgaacac gccaacaaca gcctgaacat ccaagagttt atgattatgc ccgtcggcgc
    11821 aaaatctttc cgcgaagcgt tgcgctgcgg tgcggaaatt ttccacgcct tgaaaaaact
    11881 gtgcgacagt aaaggcttcc cgaccacagt cggcgacgaa ggcggtttcg cccccaacct
    11941 gaacagccac aaagaagccc tgcaactgat ggtcgaagcg gccgaagtcg ccggctacaa
    12001 ggcgggcgaa gacgtattat tcgcattgga ctgcgcgtcc agcgagttct acaaagacgg
    12061 caaataccac ttggaagccg aaggccgctc ctacaccaac gcggaatttg ccgaatactt
    12121 ggaaggcttg gttaacgaat tcccgattat ttccattgaa gacgggatgg acgaaaacga
    12181 ctgggaaggc tggaaactgc tgaccgaaaa attgggcaaa aaagttcaat tggtcggcga
    12241 cgacttgttc gtaaccaatc cgaaaattct tgccgaaggc atcgaaaaag gcgtagcaaa
    12301 cgcattgctg gtcaaagtca accaaatcgg tactttaagc gaaaccctga aagccgtcga
    12361 tctggcaaaa tgcaaccgct acgccagcgt gatgagccac cgctccggcg aaaccgaaga
    12421 cagtaccatt gccgacttgg cagtcgccac caactgtatg cagattaaaa ccggttcttt
    12481 gagccgttcc gaccgcatgg cgaaatacaa ccaactgctg cgtatcgagg aagaattggc
    12541 ggaagccgcc tactaccccg gcaaagccgc attctaccaa ctgggcaaat aaataaaaaa
    12601 gtggatgtat gtttcaacac acagggcaac acatgaagcg tcgccctgtg tgttgacctg
    12661 atttggaaga ggttacacct ctcccaaata aagtctaatt ttaccgccac gaaggacgga
    12721 catccgagtg gtggggtttc aaccagtaag gaaacgttga tgaagtgggt aactgtcgtt
    12781 ttatccttcg cacttgtctg ttgccaatac agcctgtggt tcggcaaagg cagcatcgga
    12841 cgcaacagca gtctgagaga acagattgcc gttcaagaag aaaaaaacca gacactcgcc
    12901 ctacgcaatc attcccttgc cgccgaagtc tatgatttgg aaaacggtca agaagccatt
    12961 tcggaaatcg cccgggtaga actgggttat atccaagacg gcgaaacctt ttaccgactc
    13021 atcaggcata accggtaata ccgtcaaaaa gccgtccgaa ccaatgttcg gacggctttt
    13081 atttcaacaa actgtcagac agcccctcat cctcccccgc caaaccgcaa tccagcctga
    13141 catccccctc gacgcaacag cagcacggca gtatctcgtc ccgccccaaa aaagccaaag
    13201 gcggctcccg ataagtaacg cttccctcca aaatctttac ccggcacgat ccgcaatatc
    13261 cgcttcggca ctgatattcc accatatgcc ccgtccgttc caagccttcc aacagggtct
    13321 caccctccaa gagctcaaaa aggcccttat tcgtaccaat gcgcgccatt tccgaccaat
    13381 caaaaataaa aggaacaata accgatccta cctgctgttt ttcccatcat acaacacaca
    13441 aatgccgtct gaaacatccg gcttcagacg gcattttttc aaaaaacatt tacaactcaa
    13501 aatcgcccaa gtcatccgta ttcacttcag aatcgatttg accaatcaaa taagaggata
    13561 tttccacttc ctgcggcgcg acctgtacgt tgtcggacga cagccacgca ttaatccacg
    13621 gaatcgggtt ttgatttgcg ccttcaaatc cggccggcag ccccaccgcc tgcatacgca
    13681 gattggtaat atattcgacg tattgggata agatttcttt gttcaaaccg atcatcgaac
    13741 cgtctttaaa caaatatgcc gcccattctt tttcctgttc cgccgctttt ttgaagagtt
    13801 ggaaacattc gtcctgcaac tcggcggcaa tttccgccat ttccggatca tcaacacccg
    13861 aacgcatcag attaagcata tgctgcgtgc cggtcaggtg cagggcttca tcgcgggcaa
    13921 tcagtttgat gattttggcg ttgccttcca tcaactcacg ctcagcaaaa gcaaacgagc
    13981 aggcaaatga aacgtagaaa cggatggctt ccaacacatt gacgcacatc aggcagagat
    14041 agagtttttt cttcaacccg cgcaaagaca cagtaacggg ttttccgccg acattgtgca
    14101 ccccttcgcc caacaggttg taatattggg tgtattcgat taaatcatca taatagcagg
    14161 caatgtcttc ggcgcgggcg gtaatgtatt cgttttcgac aatatcatca aacacgaccg
    14221 acggatcatt cacaatattg cggatgatgt gggtatagct gcgcgagtgg atggtttcgc
    14281 tgaagctcca cgtttcaacc caagtttcca gctcgggaat cgaaaccaaa ggcagcaagg
    14341 caacattcgg actgcgccct tggatggaat cgagcagtgt ttgatatttc agattgctga
    14401 tgaaaatatg tttttcgtgt tcgggcaggt tggcgtagtc gatacggtcg cgcgacacgt
    14461 cgatttcttc cggccgccaa aaaaacgaca attgtttttc aatcagtttt tcaaatacct
    14521 cgtatttctg ctggtcataa cgggcgacat ttaccggctg accgaaaaac atcggctctt
    14581 tcagcgcgtc gtttttggtt ttgggaaagg tgctgtatga catgactagg tgttcgcagg
    14641 acataacgtc tctcttttta aaaattagtc gacttttcta ttaattttta attcaccata
    14701 taaaacatgt tgaattctat caataaagtg ccatcctaaa atagcaaata ctaaaagaac
    14761 actaataaat atataaaatt tatattcaac taagaatgac cgaatgacat ccatactttt
    14821 ccttttctgg aaaataacaa cttagcctca tatagtagta ggcactaatt taagaaaatc
    14881 cttgaagcct cctactatct agaagccttg gatatatttc aaaaattaac ctgttttaaa
    14941 tcttacaagc cccaccagcg cagccgtcat cttgaatatc ggtctgcgtg tcgtccgcgc
    15001 cgtcgcgggt gttgtggtag tacagggttt tgacgccgta tttgtaggca gtcagcaggt
    15061 ctttgagcat ttgtttcata gaaactttgt taccttcgaa tttgcccggg tcgtaagcag
    15121 tgttggcgga aatcgcctga tcgacgaatt tttgcattac gccgacaagt ttcaggtagc
    15181 cttcgttgcc cggaagctgc cacagggttt cgtaggcatt tttcaaggtt tcaaactccg
    15241 gcacgacttg tttcaaaatg ccgtctttcg atgctttgac cgttaccaat ccgcgcggcg
    15301 gctcgatgcc gttggttgcg ttggcgattt gcgagctggt ttcagacggc atgagcgcgg
    15361 tcagagtaga gttgcgcagg ccgtatttga cgatgtcggc acgcaggctt tcccagtcgt
    15421 aatgcaaagg ctcgccgcag acggcatcca aatctttttt gtaggtgtcg atgggcagtt
    15481 tgccttgcga ataaacggtt tggttaaaga gcgtgcacgc tccgtattct ttggcaaggt
    15541 ttgccgatgc tttgagcagg taatactgta tggcttcaaa ggtacggtgg gtcagaccga
    15601 gcgcggaacc gtcgctgtag cggacaccgt ttttcgccag atagtaggcg tagttgatga
    15661 caccgatgcc gagcgaacgc cggtccatag tagaggtacg cgcggcttct accggatatc
    15721 cctgataatc taaaagtgca tcgagtgcgc gcacggtcaa atcggcaagc ccttccaatt
    15781 cgtccaagct gtttaatgca cccaagttga aggcggacag tgtacacagg gctatttcgc
    15841 cgtcagggtc gttgatgttg tccagcggtt tggtcggcag ggcgatttcc atacacaagt
    15901 tggactgatg aacaggcgca acgcgcggat cgaacgggct gtgcgtattg cagtgatcga
    15961 cgttttgaat gtagatgcgc ccggtcccgg cacgctcctg catcagcgtg gaaaacaggt
    16021 cggcagccgg aatgatacgc ttgcgtatgt tcgggtcttg ctcgtatttc gtatagagcc
    16081 gctcaaattc gtcttggtcg gcaaaaaacg cttcgtacaa ccccgaaacc tcgttgggcg
    16141 aaaacagcgt aatgttgccg cccttaatca ggcgggtgta cagcaggcgg ttgatttgca
    16201 cgccataatc aagctggcgg atacggttgt cttccacgcc ccggttgttt ttcaacacca
    16261 gcaggctttc ggcttcaata tgccacaagg ggtagaacaa ggttgccgcg ccgccgcgca
    16321 cgccaccttg cgaacaagat ttgaccgccg cctgaaacat cttaaagaag ggaatgcagc
    16381 cggtatgccg ggcttcaccg ccccggattt cgctgtccag cccgcggata cgtccggcat
    16441 tgatgccgat gcccgcgcgt tgggaaacgt atttcacaat cgcgctggta gtggcattga
    16501 tggaatccaa actatcgtcg cattcaatca gcacacagct tgagaactgg cgcgtaggcg
    16561 tacgcacgcc gctcataatc ggcgtgggca acgatacttt gaatgtggaa acggcatcgt
    16621 aaaaccgttt gacgtaaccc aagcgcgcct ctttcgggta tttgctgaaa aggcacatcg
    16681 ccaccaaaac atataaaaac tgcggcgttt cgtaaatctg gcgggtaacg cggttctgta
    16741 ccagatattt gccttcgagc tgtttgacgg cggcatagga aaaggacata tcgcgttcgt
    16801 ggtcgatata ggcgttcagt tcgtcaaatt cttcgcggct gtaatcctca aggatatgcc
    16861 tgtcgtattt tccggcatcg gtgagctttt taacatggtt gtaaaggtgc ggcggctcgt
    16921 attcgccgta ggctatttta cgaagatgga aaatcgccaa acgtgcggca aggtattggt
    16981 agtccggggt atcttccgaa attaaatctg cagcggcttt gatgatggtt tcgtggatgt
    17041 cgtcggtgcg gatgccgttg tagaactgga tgtgcgattt caactcgacc tgcgacacgg
    17101 aaacattttc caatccttcc gccgcccaag taacgacacg gtgaatctta tccaaatcaa
    17161 tggcttccaa tcttccgtct cgtttggtta cttttaaatc agtcggtgta ttcatcgctt
    17221 cctcttccac tcttgatatt caagacacgg tcttttcaaa taaattaagg cagacaatat
    17281 agtggatttt tagcattttc tccagtcttg acaacgattg catttttcag tttcaggcaa
    17341 tgcgcgataa aacccgcctg tcgtattttt cctataacat ttgttttatc tgataattct
    17401 ttaccgataa aaaacgggta aattttttgc cttttgaccg gctccggcta caaggcgatg
    17461 aaataaggat tttccgacga aaaaggaaaa cttccggttt tccgcccctg cgaattgtta
    17521 aattttgcaa agtatgattt ttgccacgcc gccgccgaca aattccattt tcttaccgat
    17581 tggaatttat tattgagatt aatatgttat ttgaatttgc atatcaaacg gcaagttttg
    17641 tcggctgaat gagtctgaaa ctgccgacag tttgcccgtt ccatccttcc atcctatact
    17701 ggaaggaatt acaaactgaa aggatcgtca tgtccgtcaa taaagatgct tgccatactg
    17761 ttgtctgcaa tactgggact ggtatcaaca actgccgctg ccggtacgtc agaacccgcc
    17821 caccgacata ccaaacatat cagcaaggca aacaagcaga tgctgcaccc cgaatgcagg
    17881 aaatatttgg aacgccgtgc cgcgtggtac cgatcgcaag gcaacgtgca ggaattgcgc
    17941 gaaaacaaaa aggcgcgcaa agcattccgc accctgcctt atgcggaaca gaaaatccaa
    18001 tgccgggcgg cttatgaggc tttcgatgat ttcgacggcg gcaggttccg ccgttaatcc
    18061 catataaaat atgctgtctg aacacaagtt aagatggcat tttccataga tatagtggat
    18121 taaatttaga ccggtacggc gttgcctcgc cttgccgtgc tatttgtact gtctgcggct
    18181 tcgccgcctt gtcctgattt aaatttaatc cactatacaa caaactatcc ttacggcatt
    18241 tcttttgagg tactcccgtt accgcagcct taaggcatcc gcaaaagcga atcataccgc
    18301 cacattccga caaccgcttt cagacggcaa catccgcatc gcctgcgaca acggcacgga
    18361 tgctttcttc gattttatct tttaaagcat aacggtcttc gctttccgcc gcgtccgcca
    18421 cgcaaacgaa atcgactttg atagtcagtt ttttcataga cacgattcgc cacaggcaag
    18481 tcggcaaacc gacatcggca tacgacggac gggccgtcct ttttcccgtt tcgtcataat
    18541 aacgcagtgc gaccgccaaa acctttgccc ccgcatcgat ggcggattgg aacagcgcag
    18601 ccttgaacgg caggagcccc agtccggagg aagttcgcgc ctcagggaaa aaactgacgt
    18661 tttgaccgcg ttgcaaggtt tcgcagacgg cgcggttaat cggttcgatg tcgcgccgcg
    18721 aattgcggtt gatgaacacc gttcccgcgt tctgccccat cttgcccaat accggccagc
    18781 ttttgatttc ctgcttggcg ataaagctac tcggataaac cgcgctcatc gcgaaaatat
    18841 ccagccagga cacgtggttg gcggcaacca agacaccgtt cggatgttcg ggtgcgggtc
    18901 tgcccacctc caatccgata tccaaagccg ccaaagcgcc cttgcccaac gctataactg
    18961 cccgattgcg cgacttgggg cagccgccgt caataccgcg caggtttttg ccggttttga
    19021 acagccagac cgtcaagcgg cacaagcggc gcagacgtgt aaaaaatgaa gctttatttg
    19081 aagacatggg caattctttc ccgataatcg gtgtttgttg ttgaaacgtt cggacggcat
    19141 accgataaaa tgccgtcaga aaccgttttc cgccctattt ctgacagttc gtgcaataaa
    19201 acgtgccgcg ctgccccaaa gtttctttca caaccaaacc gccgcaccgg aggcacggct
    19261 gattgtgccg cccgtacacc gtatattcct gttgaaagta gccgcttttg ccgtcgctgt
    19321 ccacaaaatc cctcagcgta ctgccgcccg tttcaatggc gcgctgcaac accgctttga
    19381 cggtttcaac caaaaccgcg cactctttct tttttaggcg gttggcagga cggtggggcg
    19441 aaatgcccgc cctgaacaga ctctcgttgg cataaatatt gcccacgccg accacgacgg
    19501 tattgtccat cagagcgagt ttgaccgcgc gtttctgcgc cttcagcccc gcatacagat
    19561 aatccgtgca aaatgcctcc gacaaaggct ccggccccag tttttccaac agcggatgac
    19621 gttcttcgat tccctcatac caaagtatcg cgccgaactt tctcggatcg cggtaacgca
    19681 tgaccgtgcc gtctgaaaac acaatatcga cgtgatcgtg tctgtccggc ctgccgatac
    19741 gtccgtccga cggcgtaaag atccgcaagc tgcccgacat ccccaagtga atcagcaaaa
    19801 tccccgtttg gaaacggaca atcagatatt tggcacgcct gccgcaggac aaaacctgcc
    19861 ggccggacaa aatctccccc aaatcgggat tgatctgcca gcgcagcttc agttggcgca
    19921 atatcacggc ttccaccgtt tttccttcaa tatgcggcgc gatgccgcgc aacgtcgttt
    19981 ccacttccgg caattccggc ataacccctc ccgacatttc ttctgacaga tgccgtctga
    20041 aagacggccg tcccttaatc cgcaaccctt gccgcacccg ccgcaagggc tttgccgccc
    20101 caatacccgt cccacgcggg caggggcgtt acgcccgccg cccttttgac caaaaccaaa
    20161 ccgtacactg cggcacattg cggccaccaa cggtcgcccg ccttttccat aaaccgccaa
    20221 aagcgtattt gcccgagcga cgaaacggga ggcagataca ccatgaattt cccaaattca
    20281 atatcgaaac cggcatcggc aagctgcctt ttcaactcgg gcagcggcag acaaaaccgt
    20341 ttttccggca gacggacacc gtcaaaccaa cggcagaatc cccaaagcga atacggattg
    20401 aaacccgtca gcatcaaacg tccggacggt ttcaatatcc ggtgcgcttc cgacaggatt
    20461 tgcgaaggta cgccgcattc cagcgtatgc ggcaaaagca gcatatccgc aaaaacatcc
    20521 gccaaagcca tattctccgc cgacatcgac atatctcgcg gcacacagac aatgtcttca
    20581 gacaggctca gccactgacc gcccacctga accgcacaca ttcccgaaaa acggtatgac
    20641 gccacatacc gattaaagaa gtcctgttcc aattttgcaa cataccgccc catcgccgta
    20701 tcttcaaacc atgcatccat attgcgtccg tttcaaacag attgcctgcc gatattttat
    20761 tccaaacagg atttctgtca aaaaacacat cggccgccca tttccgaatc cgcataaagt
    20821 tcctgtaaaa cttgacgctt tttcagtcaa acagtaccat cggacgataa aatatgttta
    20881 ttccgccgag tataaatcat gtccaaactc aaaaccatcg ccctgaccgc atcaggtctg
    20941 tccgtttgtc cgggtttcct atatgcccaa aacacctcat cacaccaagt cggtttagcg
    21001 attatgcggt taaactcttc aatactcgac ctgccaccga caaaacaata tttccaatcc
    21061 ggcagcctgt gggacgagct gcgccaaggc ttccggatgg gcgaagtcaa tcccgaactg
    21121 gtacgccgcc acgaaagcaa attcatcgca agccgcagct atttcgacag ggtcgtcaac
    21181 cggagccgac cctatatgta ccatatcgcc aacgaagtca aaaaacgcaa tatgcccgcc
    21241 gaagccgccc tgcttccctt catcgaaagc gcgttcgtca ccaaagccaa atcacacgtc
    21301 ggcgcatcgg gcctgtggca gttcatgccc gctaccggca ggcattacgg cttggaaaaa
    21361 acaccggttt acgacggcag gcacgacgtt tacgcagcca ccgatgccgc actcaactat
    21421 ctgcaatatc tctatggact gttcggcgac tggccgctcg cctttgccgc ctacaactgg
    21481 ggtgaaggca acgtcggacg cgccgtcaac cgcgcccgcg accaagggct cgaaccgacc
    21541 tacgaaaacc tgcgtatgcc caacgaaacg cgcaactatg tccccaagct gctcgccgtg
    21601 cgcaacatta ttgccacccc ccaatctttc ggcatgaata tcagcgacat agacaacaaa
    21661 ccctattttc aggcagtcga accgggccgt ccgctcgaca acgaagccat cgcccggctt
    21721 gccggcatca cgcaaagcga gctgctcgcc ctgaatcctg cattcaacgt ccccgcgttc
    21781 atccccaaaa acaaacgcaa actgctgctt cctgtcgcgt ccgtccaaac cttccaaagc
    21841 aactacctca acgccgcacc cgacagcctg ttttcatggg aagtctatac gcctgccgcc
    21901 aaaaccagcc tgtccgacat ctcgacggca accggcatga gcattgccga catcaaacgc
    21961 ctcaacaacc tgaacggcaa ccttgtcaac gcaggacgca gcatccttgt cgccaagaac
    22021 ggcaagaccc ttcatacggc atcggaatcc gtcgtttcca tcgacatcga caatacgccc
    22081 gacacctacc gttccaatat gccggcaggc acggtgaacg tcagcattgc ccgaatccaa
    22141 cccgccgccg cacagacagc ggacattacc gtcgcacctt tgccgcaaga aaccgtccgt
    22201 acggaacccg atccccttgt ccgcattgcc gaacccgccc ttgcgacagc cgcagcgcaa
    22261 cctcaaaccg aaaaacagac tgccatgccg tctgaaaccc aaaccgcaac actcgcgcag
    22321 gtcgtccccc caaacgacat gcaggcggca gacgaactca tgcagcttgt tgcccgaaac
    22381 aacctgcgcc ggcaggctga agaaaccatc tccgccgtca tcggcacgcc tgacacagtt
    22441 gccgaacaca acatttcctc atctccgcaa cataccgttg ccgccgacgg caaacgccgg
    22501 gcacgtttgg aaacgcgcgt agccaaagct gccgacggcg aagccgaaac ctccccgctc
    22561 catgccagca tccaccgcgt tgtagaaggc gacaccctgt tcaacattgc caaacgctac
    22621 aacgtcagcg tagccgacct gattgtcgcc aacaacatca aaggcaacac catccaaaaa
    22681 ggacaggtac tccgcctggc gcaggcagcc cctgcccaaa cccgtattga aaaagtatcc
    22741 tacaccgcgc gcaaaggcga caccttcaaa agtatcgccg cgcgcttcaa tatccatatc
    22801 gacgacatcc gccggctcaa tcccaacctg aacaccatca atccgggaca gagggtcaaa
    22861 ctgattggaa gctgattcgg atacggcaca tgacaggact ttctcagtcc tgtctttttc
    22921 atcccacatt tcccatacca tcatgaaact catcaaatac ctgcaatatc aaggcatagg
    22981 aagccgcaag cagtgccaat ggctgattag gggcggctac gtctccatca acggaacctg
    23041 catggacgac accgatgcag acatcgattc ctcatacgtc gaaacgttgg atattgacgg
    23101 ggaagcagta accgtcgttc ccgaacccta tttctacatc ctgctcaaca agcctgaaga
    23161 ttacgaaact tcgcacaaac cgaaacacta ccgcagcgta ttcagtctgt tccccgacaa
    23221 tatgcggaac atcgatatgc aggcggtcgg caggctggat gcggatacga ccggcgtatt
    23281 gctaattacc aacgacggca aactgaacca caacctgact tcgccgagca gaaaaattcc
    23341 caagctatac gaagtaacgc tcaaacaccc tacaggagaa acgctctgcg aaactttgaa
    23401 aaacggcgtg ccgctccacg acgaaaacga aaccgtttgt gccgccgatg ccgttttgga
    23461 aaatccgacc accctgctgc tgaccattac cgaaggaaaa taccaccaag tcaaacgcat
    23521 ggttgccgcc gccggcaacc gcgtgcaaca ccttcaccgc cggcgattcg cacatttgga
    23581 aacagaaaac ctcaaacccg gggaatggaa atttatcgaa tgtccgaaat tctgaaacaa
    23641 aattccataa tttccctata ttcaaccata tgcttattaa ataagcagtt ttacaaaaat
    23701 taactcaaaa aacagttagt ttacaatata atccgcttgt ctaagtaacg cctgttcaag
    23761 caggattatg agcaagacgt tttccccgta atgtgttttg ccatctatac ttcccccctt
    23821 gtatagatgg ttttctattt caaattccct ctctttcatc atcattgaaa tgctatcatt
    23881 aatatgatta acaaaatttc acttccgtac accgatgtag aaaaataaca gattaatctg
    23941 ttcataaact ccgattcttc ctcatcggtt tatactcatc agaaatattc actgaaatat
    24001 atgcatttta catggaaaat cactaggcag ttgaaaaaca ttcagtcaat ttctgttaag
    24061 aatcacgtgt aaaatttccg ctttatctca atcggacacg gtttcaatat gtcttcccct
    24121 tcaaatacca accgtcaaac ctggtccagc cgtttgactt atatccttac cgttgccggc
    24181 gcgactgtcg gtttcggcgc gacgtggcgt ttcccgtatt tggtcggtga aaacggcggc
    24241 ggcgcgtatg tgtttttatt ctgtatcgcg atgctggtta tcggcatccc gatgattttg
    24301 gtggaaaacg tcatcggacg gcgcaaaggc gtgaacgcgc tggatgcgtt cggcggcccg
    24361 atgaacggca aacctgttgc caaaatttgg aaactggtcg gccggatggg gctgctcggc
    24421 gcgttcggca tcatggctta ttatatggta ctcggtggct gggtaatcag ctatatcgtt
    24481 aatattattg gaggaaattt gaatatttcc agccccgtcg acggtgtggt tacaaaaggc
    24541 ttctttaccg aacacattga aaacagccct tgggaaattg cgttttatac gctgcttttt
    24601 gtcgccgtga accaatggat tttggtcaaa ggcgttatcg gcggcattga aaaaacggcg
    24661 aaatacctga tgcctctgct gtttttgttc ctaatcgcga tggtcgtccg caacgttacc
    24721 cttccgggcg caatggaagg ggttgctttc tatctgaaac ctgatttcag caagattacc
    24781 gccgaactgt tcgtcttcgt tttgggacag gtattttttg ccctgagcct gggtttcggc
    24841 gtgatgatta ccttatccag ctatctggat aaaaacgaaa atctggttca gacggcagtt
    24901 atcacggcaa ttaccaatac catcatcgct gtacttgcgg gctttatgat tttcccgtcg
    24961 ctcttcagct tcggcgtcgc gcccaattcc ggcccgactt tggtgttcca aagtttaccg
    25021 attgtgttct cacatatgtg ggcaggacct gtgttcgccg tcattttctt ctctctgctc
    25081 ctgattgccg cgttgacaac ttcgctgacg atttatgaag tgttgattac gaccattcag
    25141 gaaaaaacca aaatccgccg taccgccgca atcacgattg tattgtccgt catcttcgtt
    25201 ttcggcaaca tcccgtctat tttgagctat ggcccgtgga aagacgtttc cgtgttcggc
    25261 aaaaatattt tcgatgcctt cgactacatc agcggcaaca tcttgtttat gctgaccgcg
    25321 ctcggttccg cattgtttgt cggttttgtg atgaaggacg aagcgaagga agaattgctt
    25381 tataaaggca accatacgac ggtcaatatt tggtttgcct atgtgaaata ccttgtgccg
    25441 ctggtgattc tgctgatttt cgtcagcaac ctgttctaat ccgcagcaat caatgccgtc
    25501 tgaaggtcat acctctttca gacggcattg cattgatgcc cgccacgccg gaatccgaac
    25561 cggcttgccg gcttctgtcg gacgctttcc gggcaggcgg cgttccgtct gcttagacaa
    25621 tcgtccttta aaacaggtag aatccgcccc aacgggaaac acacccttca gacggcaaaa
    25681 cccatacccc aaaccatcag gaatccccct tatgaacaga caaaaagtca tcgccatcga
    25741 cggtccgggc gcatcgggca aaggcacggt cgccgcccgc gttgccgccg cattgggata
    25801 cgattatctc gataccggcg cgctctaccg cctgaccgcc ctatatgcac aaaaacaagg
    25861 cgtggaatgg cacgatgaag aaaacgtttc cgcacttgcc aaaaaactgc ccgccgtatt
    25921 ttcaggcaac cgcatcctac ttgatggcga agacgtttca gacggcatcc ggacagaagc
    25981 catcggtatg ggcgcatccg cagttgcgca atggcccaaa gtccgcgccg cactgctgca
    26041 acgccaacgc gattttctga ccgaaaaagg actggttgcc gacggacggg acaccggatc
    26101 ggtcgtcttc ccccaagccg aactcaaaat ttttctgaca gccgaatcta aaatccgtgc
    26161 cgaacgccgc gccaaacaaa tcggcattcc ctgcgaaggt ttcacattcg agcgcatcct
    26221 gtccgacatc gagaccagag acgaagcaga ccgaaaccgc aaggttgccc ccctgaaaca
    26281 acagtccgat gccctgcttt tggatacgag ccgcctaact atagaagaaa ctgtaaaaaa
    26341 agtgcttgat tggtatcgta aagtttaaat tttcaggtat aatcgcccgc attgcgcttc
    26401 agacagcatg aatttccatg tgccgtctga aaccttattt acccgcctgc cgtgccaagg
    26461 actgcagaca cccaccaacc caacccgcac cccttggcgg tgtaccgaaa agagttatat
    26521 atgtctatgg aaaattttgc tcagctgttg gaagaaagct ttaccctgca agaaatgaac
    26581 ccgggtgagg tgattaccgc tgaagtagtg gcaatcgacc aaaacttcgt taccgtaaac
    26641 gcaggtctga aatcagaatc cctgatcgat gtagctgaat tcaaaaacgc tcaaggcgaa
    26701 attgaagtta aagtcggcga cttcgttacc gttaccatcg aatccgtcga aaacggcttc
    26761 ggcgaaacca aactgtcccg cgaaaaagcc aaacgcgcag ccgattggat cgctttggaa
    26821 gaagccatgg aaaacggcaa catcctgtcc ggcatcatca acggtaaagt caaaggcggc
    26881 ctgaccgtta tgatcagcag catccgcgca ttcctgccgg gttctttggt cgacgtacgt
    26941 cccgttaaag acacttccca ttttgaaggc aaagagatcg aattcaaagt gatcaaactg
    27001 gacaaaaaac gcaacaacgt cgttgtttcc cgccgcgccg ttttggaagc cactttgggt
    27061 gaagaacgca aagccctgct ggaaaacctg caagaaggct ccgtcatcaa aggcatcgtc
    27121 aaaaatatca ccgactacgg cgcattcgtt gacctgggcg gcatcgacgg cctgctgcac
    27181 atcaccgatt tggcatggcg tcgcgtgaaa cacccgagcg aagtcttgga agtcggtcag
    27241 gaagttgaag ccaaagtatt gaaattcgac caagaaaaac aacgtgtttc cttgggtatg
    27301 aaacaactgg gcgaagatcc ttggagcggt ctgacccgcc gttatccgca agccacccgc
    27361 ctgttcggca aagtatccaa cctgaccgac tacggcgcat tcgtcgaaat cgaacaaggc
    27421 atcgaaggtt tggtacacgt ctccgaaatg gactggacca acaaaaacgt acacccgagc
    27481 aaagtcgtac aactgggtga cgaagtcgaa gtcatgattt tggaaatcga cgaaggccgc
    27541 cgccgtatct ctttgggtat gaaacaatgc caagccaatc cttgggaaga atttgccgcc
    27601 aaccacaaca aaggagacaa aatctccggt gcggttaaat ccattaccga tttcggcgta
    27661 ttcgtcggcc tgcccggcgg catcgacggt ctggttcacc tgtccgacct gtcttggacc
    27721 gaatccggcg aagaagccgt acgcaaatac aaaaaaggag aagaagtcga agccgtcgta
    27781 ttggcaatcg atgtggaaaa agaacgcatc tccttgggta tcaaacaact ggaaggcgat
    27841 cctttcggca acttcatcag cgtgaacgac aaaggttctt tggttaaagg ttccgtgaaa
    27901 tctgttgatg ccaaaggtgc tgttgttgcc ctgtctgacg aagtagaagg ctacctgcct
    27961 gcttccgaat ttgcagccga ccgcgttgaa gacttgacca ccaaactgaa agaaggcgat
    28021 gaagttgaag ccgtcatcgt taccgttgac cgcaaaaacc gcagcatcaa actttccgtt
    28081 aaagccaaag atgccaaaga aagccgcgaa gcactgaact ccgtcaatgc cgccgccaat
    28141 gcgaatgccg gtaccaccag cttgggcgac ctgctgaaag ccaaactctc cggcgaacaa
    28201 gaataaggtt gcagacatga caaagtctga gttaatggtt cgcttggcag aagtatttgc
    28261 cgccaaaaac ggcacgcatc ttctggcaaa agacgtagag tacagcgtaa aagtcttggt
    28321 tgacaccatg acccgatcgc ttgcccgagg tcaacgcatc gaaatccgcg gtttcggcag
    28381 cttcgatttg aaccatcgtc ctgcccgcat cggtcgcaat cccaaaaccg gcgagcgcgt
    28441 ggaagtacct gaaaaacatg taccccactt caagcctggt aaagaattgc gcgagcgggt
    28501 cgacttggct ttaaaagaaa atgccaatta aaccttagca tcaaaacgcc gctggtacgc
    28561 ggcgtttttt ctttggttta acttcatccg ctgcttcaat accttaagcc aagcaagcaa
    28621 cggattagag cgtggatttt ttaccattgc ttacaatccg ctttttaaac aacaaattgt
    28681 tgatttctat tacgaacagg acaaaatcct gcttattgca ctaaaactaa gccgtttcag
    28741 gaatttgcgg caaatttaca gcttttaccg agcctaatgc tttcgctttt tggtaaaacg
    28801 ccaatttgta ttcaagcaaa tctaaataac gttttaattc ggcaatttga cacttcacat
    28861 tttctatttg attttcaaac aaggaaaggc gttcttcaat ggtatcgtcg ccaatgacgg
    28921 tacattccgc aaagcgtttg atgtctttta agctcattcc cgtatttttc aagcattgca
    28981 ataagtacaa ccattgcaaa tcgttatcgg taaaacaacg gttaccgtat tcatcacgtc
    29041 cgatattagg caacaagcct tctttgtcgt aaaagcgtaa agtgtgggcg gagatgccta
    29101 ttttttcggc agctttggca gtggtataag tcattttccc tccctctaaa caaaaaacgg
    29161 taaaaaaaca cttgccttag agtgaactct aaaatgtaaa ctgttgctat gttgctcagg
    29221 caaggctatt ttgttaatga attaagagga aagacaatgg aaatgaaaca aaccgattca
    29281 accatcaaat ctcgtgcggc ggtggcattc gcccctaacc aacccttaca aattgtggaa
    29341 atcgacgtgg aaatgccgcg taaagacgag gtgttaattc gtaacaccca cactggcgtg
    29401 tgccatactg atgcatttac cttatcagga agcgatcctg aaggcgtatt ccctgtggtg
    29461 cttggacacg aaggtgcggg tgtggtcgtt gctgtgggcg agggtgtgtc aagcgtaaaa
    29521 ccgggtaatc acgtcattcc gctttacacc gcagaatgtg gcgaatgtgg gttttgcctc
    29581 tcgggtaaaa ccaatttatg cgtctcagtg cgtgatacac aaggtaaagg cttaatgcca
    29641 gacggcacga cgcgtttttc ttatcaaggt cagccgattt atcactatat gggctgttcg
    29701 actttcagtg aatattctgt tgttgccgaa gtttcactgg cgaaaatcaa cccgaaagcc
    29761 aaccacgaac aagtatgttt gctcggctgc ggcgttacca ctggtattgg tgcggtacat
    29821 aacacggcaa aagtacaaga aggcgattct gttgccgtgt ttggcttggg agcgattggt
    29881 ttggctgtgg tgcaaggtgc gcgtcaagcc aaagccggtc gcattatcgc cattgatacc
    29941 aatcctgcaa aattcgagtt ggcaaaacag tgttggtgca actgactgtt tgagcccgaa
    30001 agattacgat aaaccaatca aagatgtgtt gttagacatc aacaaatggg gcattgacca
    30061 cacctttgaa tgtatcggca atgtaaacgt aatgcgtcag gcattagaaa gtgcacaccg
    30121 aggttgggga caatccatca tcatcggcgt agcggatgca ggacaagaaa tttcaacgcg
    30181 tccgttccaa tcggtaacag accgtgtttg gaaaggctcg gcatttggtg gtgttaaagg
    30241 ccgctccgag cttccgaaaa tggtggagga ttcaatgaaa ggcgatatcc aattagaacc
    30301 atttgtgacc catgcaatga cccttgatca aatcaatgaa gcctttgagt taatgcacga
    30361 aggtaaatcg atccgcgctg ttattcatta ctaaggtcca cgatgaaact gattgaacaa
    30421 catcaaattt ttggtggttc gcaacaagtt tgggcgcatc atgcccaaac gcttcaatgc
    30481 gaaatgaaat ttgccgtcta tttgccggat aatccggaaa atcaaccgct tggtgtgatt
    30541 tattggcttt ccggcttgac gtgtacggaa caaaatttca ttaccaaatc gagcttccgg
    30601 cgttatgcgg cagaacatca agtggttgtg gtcgcccccg ataccggccc tcgcggagag
    30661 caagtgccga acggtgccgc ttatgattta ggacaggggg caggctttta tttgaatgcg
    30721 accgaacagc cttgggcggc gaattatcaa atgtatgatt acattttgaa cgagttgccc
    30781 cgtctgattg aggagcattt tcctaccaac ggcaaacgtt ccattatggg gcattcgatg
    30841 gacggacacg gcgcattggt attggcacta cggaatcggg aacattatca aagtgtttct
    30901 gccttttcgc ctattttatc gccaagcctc gtaccgtggg gagaaaaagc ctttactgct
    30961 tatttaggga aagaccgtga aaaatggcag caatatgatg ctaactcact cattcaacaa
    31021 ggctataaag tgcaaggtat gcgtatcgat cagggcttgg aagatgagtt tttgccgaca
    31081 caattgcgta ccaaagattt tatcgaaacc tgtcgtgcgg caaatcagcc aatcgatgtg
    31141 cgtttccata aaggatacga tcacagctat tacttcatcg ccagttttat tggtgagcat
    31201 atcgcttatc acgccgcatt tttgaagtaa gtcaaagagc ggtcagtttt caaagcagtt
    31261 tggaatagcc ggcacgaggg cggtaagaag tgccggcata aacgtatgcc gcctgaaccg
    31321 gaaggagccg gccctacgga ttaccaaacc ctgcccttga tttgattagt atgattttta
    31381 agcttaaacc ctgattgatc aaacattaaa atcgcccgta tttcctactt cactttcggg
    31441 cttcgtatta ttcgttgccg aatttgcata agcaacctgt tcctgttgtg ttttcgcccc
    31501 ctccaagctg acggtggcgt aacagcttcg cgattcacca tctgtttcat ttgtcctcct
    31561 tcaatattaa aacgcagcct gaaataaaag gcattttggc tcatatctgc accatattaa
    31621 aacgccgcct ttgcttatac cccctttgtg cgcgtcatta ttcttttcca cgggaaacgc
    31681 caagtttgaa ggaaatcatt tataatacca gcggtaagca ttttctttct tagccgcaag
    31741 aagtataaca aggttaaata tgaataatag agaccaactt tttaaagccc cgccgtttga
    31801 aaaccacagc ccgctgacct ggtatcaggc tgcctcacaa ctgcccaact tcatccgcga
    31861 cgacgcacag gcggccgcca tcgaacacct tgaccggctt tggaccgaat tgatgatgtt
    31921 caaacgcaaa agaaaccgtt ttttaggcag gagtttgcgt tccccgcaag ttcccaaagg
    31981 gctttatttc tatggcggag tcggacgcgg caagagcttt ctgatggacg cttttttcgg
    32041 ctgcctccct taccgccgca aacgccgcgt ccactttcat gcctttatgg cggaaatcca
    32101 ccggcggctg aaagccctga aaagcgaaag caacccgttg aaatccgttg ccgccgagat
    32161 taccaaagaa acccgagtat tgtgttttga cgaatttcat gtcagcgata ttgcggatgc
    32221 aatgatttta ggccgtctgc tggaaaacct gcttaacgag ggcgttgttt tggtggcgac
    32281 ttcaaactac gcgccttccg aactctaccc gcaaggtcaa aaccggagcg gttttcttcc
    32341 cacaatcgcg ctcatcgagt ccagtctgac cgtcttaaac gttgacggag gggaagacta
    32401 ccggctgcgt accctccgcc ccgccgagat tttctttacg cctgccaatg aagaaaatga
    32461 ggcaaaactg gcaaaactgt tcaaagaaat gacaggcatt accgatttga accccggcat
    32521 cagcaccatc cacggccggg aaattcccca caaagccgag tccggtcgta ccatatggtt
    32581 tgatttccgc gcactgtgct tcagtccccg ctcacagtcc gactatctgt atttggccga
    32641 acattatgaa atggttttta tttcaggttt ggaacaactc tcaccgcaag aaaaggcgga
    32701 ggcgcgacgg ctgacttggc tgattgacgt actctacgat ttccgggtca aactgtgtgc
    32761 caccggcgcg gtagatgtca accatatcta cacggaaggc gattttgccg aagaatttac
    32821 ccgcaccgcc agccggatgg tcgaaatgca gtccgaagtt tatttggaac agccgcacct
    32881 gaccttatct cccaaggctt caggcggata agttattttt ttggtagaat accgatttga
    32941 ttcttcttaa gtaaaaataa ggatatagca tggcgattga acgtaccata tccatcatca
    33001 aacccgatgc cgtcggcaaa aacgttatcg gcaaaatata cagccgcttt gaggagaacg
    33061 gtctgaaaat cgttgccgcc aaaatgaagc agcttaccct caaagaagcc caagaatttt
    33121 atgcggttca taaagaccgc cccttctatg ccggattggt tgaatttatg accggcggtc
    33181 cggttatgat tcaagtactg gaaggcgaaa acgccgttct gaaaaaccgc gaactgatgg
    33241 gggcgaccaa tcccaccgaa gccgcagaag gcacgatacg cgcggacttt gccacttcgg
    33301 tcagcattaa cgccgtacac ggttccgaca gtgtggaaaa tgccgctttg gaaattgcct
    33361 actttttcag ccaaaccgaa atctgccccc gttgatacaa tacaccgccc aattcctatt
    33421 cagacggcat aaaatatatc catgccgtct gaaaactctg ttgcaaaaga cttcaaatca
    33481 aacttgcctg ccctgcaatt ttttatttga agccttaatt taagaaaaac acaaatacat
    33541 gaaaaccaat ctgctcaact acgaccttca agggctaacc cgacattttg ccgatatggg
    33601 tgaaaaacca ttccgcgcca aacaggttat gcgttggatg caccaatccg gcgcgcaaaa
    33661 ttttgacgaa atgaccgatt tggcaaaatc gttgcgccat aaactgaacg aacaggcaag
    33721 catcgaaatt cccaagctga tgatgtccca agaatcttca gacggcactc gaaaatggct
    33781 tttggatgtc ggtacgggga acggcgtgga aaccgtcttc atccccgaat cggatcgcgg
    33841 cacgctctgc atttcctcgc aagtcggctg cgctttggaa tgtacatttt gttcgaccgg
    33901 ccggcagggc ttcaaccgca atttaaccgc tgccgaaatc atcggacagt tgtggtgggc
    33961 aaacaaagcg atgggcgtta caccgaaaaa cgagcgtgtg atttccaacg tcgtcatgat
    34021 gggcatgggc gagccgatgg cgaacttcga caatgtcgtt accgccttga gcatcatgct
    34081 ggacgaccac ggctacggtt tgagccgccg ccgcgtaacc gtttccactt cgggtatggt
    34141 tccccaaatg gacaggttgc gcgatgtcat gccggtggcc ttggcggttt ccctccacgc
    34201 ttccaatgac gaagtccgca accaaatcgt accgttgaac aaaaaatatc ccttgaaaga
    34261 attgatggcc gcatgccaac gctatctggt caaagcgccc agggatttca tcactttcga
    34321 gtatgtgatg ctggacggtg tcaacgataa ggcacaacat gcatacgaac ttattgaact
    34381 ggtcaaagat gttccctgca aattcaattt aatcccgttt aatcctttcc ctaattcggg
    34441 atatgaacgt tcaagtaacg aaaacatccg tattttcaga gatatcctgc aacaagccga
    34501 atttgtcgtt accgttcgaa aaactcgtgg cgacgatatc gatgccgcct gcggacagtt
    34561 ggcaggtcag gttcaggata aaacgcgccg ccaacaaaaa tggcagcaga ttttaatcgg
    34621 acaacagggg taattatgcc ttttaagcca tccaaacgaa tctctttatt acttgttctt
    34681 gccttgggcg cgtgcagcac ttcctaccgc ccctcgcggg cagaaaaagc caatcaggtt
    34741 tccaatatca aaacccagtt ggcgatggaa tatatgcgcg gtcaggacta ccgtcaggca
    34801 acggcaagta ttgaagatgc cttgaaatcg aaccctaaaa acgagcttgc ctggctggtc
    34861 cgtgccgaaa tctatcaata cctgaaagtt aacgacaagg cgcaggaaag tttccggcaa
    34921 gccctctcca tcaaacccga cagtgccgaa atcaacaaca actacggctg gttcctgtgc
    34981 ggcaggctca accgccctgc cgaatctatg gcatatttcg acaaagccct ggccgacccc
    35041 acctacccga ccccttatat tgccaacctg aataaaggta tatgcagcgc aaaacagggg
    35101 caattcggat tggcggaagc ctatttgaaa cgttccctcg ccgcccagcc gcagttccca
    35161 cccgcattta aagaactggc gcgcaccaaa atgctggccg ggcagttggg cgatgccgat
    35221 tactacttta aaaaatacca aagcagggta gaagtccttc aggccgatga tttgctgcta
    35281 ggctggaaaa ttgccaaagc cctcggcaac gtgcaggcgg catacgaata tgaagcacaa
    35341 ttgcaggcaa atttccccta ctcggaagaa ttgcaaaccg tcctcaccgg tcaataaaca
    35401 gattcaaact atatgaacac actccaacgc cgcaagacgc atcaagtcct catcgatcat
    35461 attaccgtcg gttcagaagc acccgtcgtt atccaatcta tgaccaacac cgacactgcc
    35521 gatgcaaaag ccaccgcatt gcagattaag gaattgagcg atgccggctc ggaaatggtg
    35581 cggattaccg tcaacagccc cgaagccgcg tccaaagttg ccgaaatccg ccgccgcttg
    35641 gacgatatgg gctatgccac accactgatt ggcgatttcc attttaacgg cgaacgcctg
    35701 ttggcggaat ttcccgaatg cggcaaagca ttgtccaaat accgcatcaa tcccggcaat
    35761 gtcggcaaag gcgtaaaagg cgatgaaaaa tttgccttta tgattcggac tgctgctgaa
    35821 aacgataaag ccgtccgtat cggcgtaaac tggggttctt tggatcagag cctcgccaaa
    35881 cgtatgatgg atgccaacct cgtttcttcc gcgccgaaac cgcccgaaga agtgatgaag
    35941 gaagcactga ttgtctccgc tttggaatct gccgaaaaag ccgttctatt gggactgccc
    36001 gaagacaaaa tcatcctgtc gtgcaaagtc agcgcggttc atgatttgat tcaggtttac
    36061 cgcgaactgg gcagccgttg cgtctatccg ctgcatttgg gtttgaccga agccggtatg
    36121 ggcagcaaag gcattgtcgc atcaacggcg gcattatctg ttttgcttca agaaggaatt
    36181 ggcgacacca tccgcatttc actcactccg gaaccgggca gcccgcgtac tcaggaggtc
    36241 gtcgtcgggc aagagatttt acagactatg ggtttgcgct cgtttacgcc gatggttacc
    36301 gcctgcccgg gatgcgggcg taccaccagt accgtatttc aagagctggc acaagatgtt
    36361 caaaattacc tgcgccaaaa aatgtctata tggcgtaccc tttatcccgg ggttgaatcc
    36421 ctgaacgttg ccgtaatggg ctgcgttgtc aatggccccg gagaaagcaa attggccgac
    36481 atcggaatca gccttcccgg tacgggggaa acacccgtcg cacctgttta tgtagatggg
    36541 gagcgcaaag tcacactgaa aggtaataat attgcaagcg aattcctagc tattgtagag
    36601 gaatatgtta aaaccaatta tggcaaaaac agttctaaat gcaataaagg aaaagtcatc
    36661 ccgatacagt ctctataaaa caaatgccgt ctgaacgttt cagacggcat ttgtttctct
    36721 cagatttctt attaaagccg caaagaagcg cggttttcca aaacttggtc gatcaaacca
    36781 tattcttttg cttcttcggc agacatgaaa ttatcacggt cggtgtcgcg ctccaaatct
    36841 gccaaatcgc ggccgcaatg tttcgccatc aggcggttga gtttttcttt gattttcaac
    36901 aactcgcgtg cgtggatttc aatgtcggat gcctgaccgc ccaagccgcc gctgattaaa
    36961 ggctggtgaa tcataatccg gctgttgggc agggcgaaac gtttgccttt ctcgcctgcc
    37021 gacaataaga acgcgcccat acttgccgcc tgccccaagc acaaagtcga tacatcgggc
    37081 ttgatgaaat tcatcgtgtc gtaaatcgac ataccggccg ttaccgagcc gccgggggaa
    37141 ttgatgtaga agaaaatatc cttatccgga ttctcacttt ccaaaaacaa cagttgggcg
    37201 accaccagat tagcagactc atcggttacc gggccaacca gaaatacgat gcgctctttc
    37261 aaaagccggg aatagatatc gaatgcacgc tcaccgcgac cgctctgctc gataacggta
    37321 gggacaagat ggttatcaaa agacatttcg tctcctttca tgatggaaaa gcaccaaagc
    37381 gggctttaaa aacggcttcg gtgctttcaa aaactgcctt cagacagcat tttcagaaca
    37441 atcaggcttg cgcgcccatc acttcgtcaa aagacaaagc ttttttgttt actttggctt
    37501 tgcccaaaac gaaatcaaca acgttgcttt ctactgccaa agaagtcggg gcttgcaggc
    37561 gggaagtatc tgcgtagtac cagtcaatca cttcttgagg atcttcgtag ctttctgcga
    37621 agttggcgac aacggctttg atttgctctt cagtcggttc cagtttgttt tcgtcaacca
    37681 gtttggccaa aatcagaccc aaagatacgc gacgttcggc ttgttctttg aacatatcca
    37741 aaggcaaatc caagttcgcg gcatcggtca taccttggtt aacgaagttt tgtttcattt
    37801 cgtttgccag gcgggcggct tcttcattga ccaaagcaac cggaactttc aactctacgg
    37861 ctttaatcag cgcgttcatt acggattctt tggtttgttc gttcacgcgg cgttccactt
    37921 cgcggcttac gtttttcttc acttcttcac gcattttggc aacatcgcca tccgcaatac
    37981 ccaaggcttt tgcaaaatct gcatcgactt caggcagagt gggctcggaa acattgttca
    38041 gcgtaatggt gaacacggca gttttaccgg caacatcttt gccgtggtat tcttcaggga
    38101 agttgacggt aacgtcttta ctttcgcccg ctttcatgcc gactacgccg gcttcaaatt
    38161 caggcagcat ttgacctgcg cccaatacga aggcgtagtt tttggatgcg ccgccggcaa
    38221 aaggttcgcc gtcgattttg ccttcaaagt cgatgatgac gcggtcgccg tttcgggctt
    38281 cgcggtcaac atggttgaag cgggtacgtt gtttacgcag gatttctaca gtttggtcga
    38341 cttcggcatc gccgacggaa gcggttactt tttcgacctc ttgtgcagac aaatcgccga
    38401 taacgacttc ggggaacact tcaaaaatgg cggcaacttt gaaagactct ttatcgtctt
    38461 gttcttcaac gccttcaaaa cgtggatagc ctgccacttt caactcttgg gcaacggcaa
    38521 catcgtagaa gcggcgttgc accagctcgt tgatcacgtc gttttgcgcg ctcgcaccgt
    38581 acatttgggc aatcattttt aaaggtgctt tacccggacg gaaaccgtcg atttttgcac
    38641 ggcgttgggt ttgtttcagt tttttatcgg tttctgcgtt gatttcggac caaggcaggg
    38701 acaacactac tttgcgttcc agattttcta aaatttcaac agttacgctc atcataagcc
    38761 cttaaatttg ttgtgttgat aaaatgataa accttcttcc ctacatgggg aagcaaacag
    38821 cgcaacggta cgatatttga accgcattgc cgcaaagggg aaattttagc tggcaagtat
    38881 atcacaatgt ttcgcctgaa acataatatg c
//
LOCUS       DAXFWM010000013        38158 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000013 DAXFWM010000000
VERSION     DAXFWM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38158
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.013"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(170..661)
                     /gene="dtd"
                     /locus_tag="ACFI8B_000622"
     CDS             complement(170..661)
                     /gene="dtd"
                     /locus_tag="ACFI8B_000622"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC3833908.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(735..1493)
                     /locus_tag="ACFI8B_000623"
     CDS             complement(735..1493)
                     /locus_tag="ACFI8B_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3833909.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(1563..2429)
                     /locus_tag="ACFI8B_000624"
     CDS             complement(1563..2429)
                     /locus_tag="ACFI8B_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3833910.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(2478..2756)
                     /locus_tag="ACFI8B_000625"
     CDS             complement(2478..2756)
                     /locus_tag="ACFI8B_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC3833911.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(2756..3046)
                     /locus_tag="ACFI8B_000626"
     CDS             complement(2756..3046)
                     /locus_tag="ACFI8B_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC3833912.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(3049..3579)
                     /locus_tag="ACFI8B_000627"
     CDS             complement(3049..3579)
                     /locus_tag="ACFI8B_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC3833913.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            3887..6202
                     /gene="tspA"
                     /locus_tag="ACFI8B_000628"
     CDS             3887..6202
                     /gene="tspA"
                     /locus_tag="ACFI8B_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC3833914.1"
                     /translation="MKLIAASIAVAASFQAHARLGGLNIRSNLDEPFSGSITVTGEEA
                     KALLGGGSVTVSEKGLTAKVHKLDGKAVIAVSSAQAVRDPVLVFRIGAGAQVREYTAI
                     LDPVGYSPKTKSALSDGKTHRKTAPKAESQENQNAKALRKTDKKDSANAAVKPAHNGK
                     THTVRKGETLKQIAAAIRPKHLTLEQVADVLLKANPNVSAHGRLRAGSVLHIPNLNRI
                     KAAAPKKIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPIEKPVEKPVEKPEAKVAA
                     PEAKAEKPAVRPEPKPAVSETPASATERQPGPVPAANTAASETAAESAPQEAAASAID
                     TPTDETGNTVSEPVAETASEVAAEPVAEPVEQVSAEEETESGLFDGLFGGSYTLLLAG
                     GGTALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIKITFAEVETPATPEPA
                     PKNDVNDTLALGGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQD
                     EETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTD
                     FYNNLFSNNHIGTEETASAKPAAPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLK
                     VYETGAVAETAPETPDFNAAADDLSALLQPAEAPAVEENAAEITLETPDSNTSEADAL
                     PDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSDAPSEAKYDLAEMYLEIGDR
                     DAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(6375..6791)
                     /gene="gloA"
                     /locus_tag="ACFI8B_000629"
     CDS             complement(6375..6791)
                     /gene="gloA"
                     /locus_tag="ACFI8B_000629"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC3833915.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(6816..7985)
                     /gene="lapB"
                     /locus_tag="ACFI8B_000630"
     CDS             complement(6816..7985)
                     /gene="lapB"
                     /locus_tag="ACFI8B_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC3833916.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(7996..8310)
                     /locus_tag="ACFI8B_000631"
     CDS             complement(7996..8310)
                     /locus_tag="ACFI8B_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC3833917.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            8620..9618
                     /gene="ilvE"
                     /locus_tag="ACFI8B_000632"
     CDS             8620..9618
                     /gene="ilvE"
                     /locus_tag="ACFI8B_000632"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC3833918.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(9863..10648)
                     /gene="fabI"
                     /locus_tag="ACFI8B_000633"
     CDS             complement(9863..10648)
                     /gene="fabI"
                     /locus_tag="ACFI8B_000633"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC3833919.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(10803..11624)
                     /gene="dapD"
                     /locus_tag="ACFI8B_000634"
     CDS             complement(10803..11624)
                     /gene="dapD"
                     /locus_tag="ACFI8B_000634"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC3833920.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(11834..13477)
                     /gene="pgi"
                     /locus_tag="ACFI8B_000635"
     CDS             complement(11834..13477)
                     /gene="pgi"
                     /locus_tag="ACFI8B_000635"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC3833921.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            13987..15219
                     /gene="pilG"
                     /locus_tag="ACFI8B_000636"
     CDS             13987..15219
                     /gene="pilG"
                     /locus_tag="ACFI8B_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC3833922.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            15293..16153
                     /locus_tag="ACFI8B_000637"
     CDS             15293..16153
                     /locus_tag="ACFI8B_000637"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC3833923.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            16150..16782
                     /gene="coaE"
                     /locus_tag="ACFI8B_000638"
     CDS             16150..16782
                     /gene="coaE"
                     /locus_tag="ACFI8B_000638"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC3833924.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            16775..16984
                     /locus_tag="ACFI8B_000639"
     CDS             16775..16984
                     /locus_tag="ACFI8B_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC3833925.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(17070..18746)
                     /gene="pilB"
                     /locus_tag="ACFI8B_000640"
     CDS             complement(17070..18746)
                     /gene="pilB"
                     /locus_tag="ACFI8B_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC3833926.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(18992..19063)
                     /locus_tag="ACFI8B_000641"
     tRNA            complement(18992..19063)
                     /locus_tag="ACFI8B_000641"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19028..19030),aa:Arg,seq:cct)
     gene            complement(19084..19530)
                     /locus_tag="ACFI8B_000642"
     CDS             complement(19084..19530)
                     /locus_tag="ACFI8B_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC3833927.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(19534..20508)
                     /locus_tag="ACFI8B_000643"
     CDS             complement(19534..20508)
                     /locus_tag="ACFI8B_000643"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC3833928.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            20732..21040
                     /gene="rplU"
                     /locus_tag="ACFI8B_000644"
     CDS             20732..21040
                     /gene="rplU"
                     /locus_tag="ACFI8B_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC3833929.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            21065..21337
                     /gene="rpmA"
                     /locus_tag="ACFI8B_000645"
     CDS             21065..21337
                     /gene="rpmA"
                     /locus_tag="ACFI8B_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC3833930.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            21572..22756
                     /gene="ubiM"
                     /locus_tag="ACFI8B_000646"
     CDS             21572..22756
                     /gene="ubiM"
                     /locus_tag="ACFI8B_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC3833931.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(22945..23100)
                     /gene="rpmG"
                     /locus_tag="ACFI8B_000647"
     CDS             complement(22945..23100)
                     /gene="rpmG"
                     /locus_tag="ACFI8B_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC3833932.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(23132..23365)
                     /gene="rpmB"
                     /locus_tag="ACFI8B_000648"
     CDS             complement(23132..23365)
                     /gene="rpmB"
                     /locus_tag="ACFI8B_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC3833933.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(23615..25138)
                     /gene="farB"
                     /locus_tag="ACFI8B_000649"
     CDS             complement(23615..25138)
                     /gene="farB"
                     /locus_tag="ACFI8B_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC3833934.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGITALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(25162..26316)
                     /gene="farA"
                     /locus_tag="ACFI8B_000650"
     CDS             complement(25162..26316)
                     /gene="farA"
                     /locus_tag="ACFI8B_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC3833935.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGGVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      26557..26601
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            26605..27078
                     /gene="queF"
                     /locus_tag="ACFI8B_000651"
     CDS             26605..27078
                     /gene="queF"
                     /locus_tag="ACFI8B_000651"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC3833936.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            27153..27839
                     /locus_tag="ACFI8B_000652"
     CDS             27153..27839
                     /locus_tag="ACFI8B_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC3833937.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(28184..29476)
                     /locus_tag="ACFI8B_000653"
     CDS             complement(28184..29476)
                     /locus_tag="ACFI8B_000653"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC3833938.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGGTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            29553..30521
                     /locus_tag="ACFI8B_000654"
     CDS             29553..30521
                     /locus_tag="ACFI8B_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833939.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWGERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(30722..32191)
                     /locus_tag="ACFI8B_000655"
     CDS             complement(30722..32191)
                     /locus_tag="ACFI8B_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3833940.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGLSVTREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRAYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(32433..32690)
                     /locus_tag="ACFI8B_000656"
     CDS             complement(32433..32690)
                     /locus_tag="ACFI8B_000656"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3833941.1"
                     /translation="MRAKTRFALRNGVNLQPFAAFNVLHRSKSFGMEMDGEKQTLAGR
                     TALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSVKWLF"
     gene            32616..32777
                     /locus_tag="ACFI8B_000657"
     CDS             32616..32777
                     /locus_tag="ACFI8B_000657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833942.1"
                     /translation="MQNIKSGKRLKIDTVTQSKTGFCPNAGADLPLTAAEQPDRRPSL
                     SVKPPFTPK"
     gene            complement(32874..33287)
                     /locus_tag="ACFI8B_000658"
     CDS             complement(32874..33287)
                     /locus_tag="ACFI8B_000658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833943.1"
                     /translation="MSFDCFGAFSYQPGSDTRPTNVGDTADFSFSDKPANGVFHYFSS
                     GKTDQKSSEALYDEINITDNYRTDLKQAVKKQLQDLYKTRPEAWEENKKRVREEIIKQ
                     FGISEDVFNKLQQKMPQKINSLIEEQVLTPYILRP"
     gene            complement(33814..34059)
                     /locus_tag="ACFI8B_000659"
     CDS             complement(33814..34059)
                     /locus_tag="ACFI8B_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833944.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            34171..34599
                     /locus_tag="ACFI8B_000660"
     CDS             34171..34599
                     /locus_tag="ACFI8B_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC3833945.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            34611..35087
                     /locus_tag="ACFI8B_000661"
     CDS             34611..35087
                     /locus_tag="ACFI8B_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC3833946.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(35117..35605)
                     /locus_tag="ACFI8B_000662"
     CDS             complement(35117..35605)
                     /locus_tag="ACFI8B_000662"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC3833947.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEVALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWREAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(35673..36728)
                     /gene="aroG"
                     /locus_tag="ACFI8B_000663"
     CDS             complement(35673..36728)
                     /gene="aroG"
                     /locus_tag="ACFI8B_000663"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC3833948.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(36890..37117)
                     /locus_tag="ACFI8B_000664"
     CDS             complement(36890..37117)
                     /locus_tag="ACFI8B_000664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833949.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            37345..37641
                     /locus_tag="ACFI8B_000665"
     CDS             37345..37641
                     /locus_tag="ACFI8B_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833950.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            37638..37877
                     /locus_tag="ACFI8B_000666"
     CDS             37638..37877
                     /locus_tag="ACFI8B_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833951.1"
                     /translation="MSGVAVYLRNKNRIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVSNTTKTKTDSEKFNE"
BASE COUNT         9112 a        10861 c         9413 g         8772 t
ORIGIN      
        1 tttgcacctt atttgcacct tttttataaa tataattcaa atatttgatt tatatattaa
       61 tttaattaaa tattacatga ccctgcatag gggcgacgga aaggcggcgc gggggaaggg
      121 tgtgtgtatt gtcgttcatt gtcgatattt catcattgcg gactcaattt caatccggaa
      181 caaccttcat ttttggggaa atccgcgtca tgaaagagtc cagcagtatg gttaccggcc
      241 cgtcgttgca gagcgacacc tgcatatgcg tgcggaaacg ccctgtttcg acatgaatcc
      301 cgtgtccgcg taacagctcc gccgttcgca ggtaaagccg ttgcgcctgt tctgccggcg
      361 cggcttggga aaacgaaggc cgccgcccgc ttgccgcgtc ggcataaagc gtaaactgcg
      421 acaccagcag caccgcgccg ccgacatctt tcaaagacag gttcagcttg cccgcttcgt
      481 cttcaaacac gcgcaaatgt gcgattttgt cggcgatata gcgtgcatct ttttctgtgt
      541 cgctatgcgt tacaccgagt aacacgacaa acccgccgtc gattttgccg caggtttccg
      601 tgccggcttc ggacacgaca tccacctttg cacctaccgt tttctgtatg accgcacgca
      661 taagccgtcc tccttacctg aacgtgcaat gccgtctgaa aaggcttcag acagcatcgg
      721 tatcggaaat gccattacgg tttgacaccg ttttcttcca aaagggcatc gattttcaag
      781 cgggcttttt cctgcctcaa accttgttcc aactggtttc tgaccaactc gaaaggctgc
      841 gcgtcggggt ttttcccgac cgcgccgagt ttgaacaggt aatagcgttc gcccaatttg
      901 accggattgc gggtaacgtc gccacggttc ataccggcaa actgcgaagc cagcggctcg
      961 ggaagctgct gcgccataat gaaaccgtcg aacgcctgct cgtcgttcgg ataacgcttc
     1021 atcagccctt caaaagacag ccctttgagc aggagctgct gcgcctgacg cgcctcctct
     1081 tcggttgcga agctgacctg ctgcaatttg atcatgcgga tttgccgctc ataaaactga
     1141 cgcagtgcgc tttcggaaac cgtttccgaa cgttccagaa aacggacgta ctcctcggca
     1201 taaaaagacg cttcggcgat tttaaagcgg ttttggacat ccttatcctt atccaaacct
     1261 tccttcaatg ccctgttttt caaaacttcc aaagtttgca gccggcggac ggcatcgttt
     1321 cggattgcct gcccgtccgg tctttgggac tgctccgcat gccggtctgc ctgctgcatg
     1381 atctgcgcca ccagcgtatc caccaaagcc gggtcgattt cgggcgcttt ggctgccgca
     1441 aaacctgcca acattgcagc aataactgcg gcagcggtct ttttctgttt catggtattc
     1501 cctgtcctgc gcggcggcaa aacgcctgta tgaacgtctt gccgccgtat cccgttttca
     1561 gattattttg caggtttgat gtttgcctta cccaacagcg caccgacggc acggtcaatc
     1621 cgttccgcct gaaggttgcc ggcaatctgt cctttcattt cgtcaaaaga aggcactttc
     1681 acctcgcggc tgtcgttgac ataataaacg ccgtagaaat cgccgttttt cagcggcgtt
     1741 gccgtaaatt cgcctttttt caagtcctta attgcctgat aaagcggcgg aacaccctgt
     1801 tccaaatctt tcagcggcac atatccgtcc ggcgcgccgg tccgtttggt gcggtcgttg
     1861 agcgagtatt gtttcaaaac ggcatcaaaa ccttttttcg ccttcaaatc ggcaaccgct
     1921 tttttcgcat tttcttcctt gtcggtcagg atttcgccca actggacttc ctgcgtgcct
     1981 ttataaaaac cgctgatatt gtcgtaaacg gcttttactt cctgctcgga aaccggttgg
     2041 gttttggcga tatgcagtgc gtatgcctcg ccgttcaagc catattttac cgcctgccaa
     2101 acggttttga aggacggttt cttgtcgtcg cccgactttt tcgcttcggc acgcaatttg
     2161 gcaagcgcat ctttaaactc tgccgaccgg tcgagtttca ggcgtttcac ttcctgtgcg
     2221 accacggtgt tgaccacttc gttttccagc agggattggc gcagttgcgg cgtgtcttcg
     2281 gcacggctgt tttccgcacg gaatgcggca acctgcgcat cgatgacgga actgtcgatt
     2341 ttctgaccgt taacggttgc cagcgtttgg gcaaacaggc tgccggaaca ggcaagcagc
     2401 gcaacggaag tcaggatttt tgctttcata atcttctctc tttaaatttt aaattgaaat
     2461 attcaggcag acttcattca gtccgccgta tggaaatact cgtcaggggt agccgcccgg
     2521 atgccgagcg cgtgaatcat gccgcctgaa aataaatctt tgagcagcga tttgaccgtt
     2581 ttctgacggt tcagacggct taaaccttca aaacggccgc tgacgaccaa aacggcataa
     2641 tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat ggctctcatc gccgatttcc
     2701 aacaccagag gatcgagcgt ctgcaggcgt tcgcggatca aatcgacagc cggcattatt
     2761 tgaacaccgc tttaaacggc ttgatcagca cttcgctgta cacgccggca tgaacatagg
     2821 gatcgtcttc agcccaagcc tgcgccgcat ccaaagactc gaactgtgcc acaatcaagc
     2881 tgcccgaaac acgttcggga ttgtccggca gcaggtttgg gcctgccgtc agcagacggc
     2941 cttccgattt cagcgtttcc agccgtttaa agtgttcggg acgtgccgcc atacgcgctt
     3001 catgcacatc ctccccgtct gttgccagca acataaaata ttccacagtc aatcctcctt
     3061 tttcagatag gtactcagat aaataccctg aataataaag aaaaaaagca tcagcgcagt
     3121 cgaaccgaac atcttatagt taacccattg cgcttcaaac ctagtaaaca caaaccagtt
     3181 ggcaataccc ataaaaatca gaaaaccgac ccacatatat gtcaattttc cccatacggc
     3241 atccggaagc tgaatctccc tgccgatact cgctttcaag ccgtttttac ccgcaaggtg
     3301 gctgcccagc aggaataacg ccccgcacca gaacaatact gtcggcttcc acataatgaa
     3361 gcggctgtcg cccaaaacaa tggttgcgcc gccgaatacg acaatcagca ccagtccgac
     3421 ccactgcatc gtatccagcc ttttatgctt ccaatacagg aaagccgcct gaaccacgcc
     3481 tgcaaccaag gcaaccgccg cagcggcaat catatttttg gtaacggtat aagtagcaaa
     3541 aaacaagatg acggacaaaa ggtcgctgac aaatttcata tccaaatgag aaagcaaatc
     3601 aaataaacgc taatcataca ggcagaaccc tatctttgca cctgcaaaac caacaaattt
     3661 tgttttgatt aatgcaaata tgcacagatg tttttgaaaa aagatggaaa tatgtcataa
     3721 tttataaaat tggctttaaa atacagcttc ggccggaacc tctgcctatc cgcaccctat
     3781 cgtccgtatt gcctatgcct gatgacgaaa tttacagact gtacgcggtc aaaccgtatt
     3841 cagccgccaa cccacagggg atacatcttg caaaaacaac agacaaatca aactgattgc
     3901 cgcctccatc gcagttgccg catcctttca ggcacatgcg cgactgggcg gactgaatat
     3961 ccggtccaac cttgacgaac ccttttccgg cagcattacc gtaaccggcg aagaagccaa
     4021 agccctgcta ggcggcggca gcgttaccgt ttccgaaaaa ggcctgaccg ccaaagtcca
     4081 caagttggac ggcaaagccg tcattgccgt ttcttccgca caggcagtcc gcgatcccgt
     4141 cttggtgttc cgcatcggtg caggcgcgca ggtacgcgaa tacaccgcca tcctcgatcc
     4201 tgtcggctac tcgcccaaaa ccaaatctgc actttcagac ggcaagacac accgcaaaac
     4261 cgctccgaaa gcagagtccc aagaaaatca aaacgccaaa gccctccgca aaaccgataa
     4321 aaaagacagc gcaaacgcag ccgtcaaacc ggcgcataac ggcaaaaccc ataccgtccg
     4381 caaaggcgaa acgctcaaac agattgccgc cgccatccgt ccgaaacacc tgacgctcga
     4441 acaggttgcc gatgtgctgc tgaaggcaaa cccaaatgtt tccgcacacg gcagactgcg
     4501 tgcgggcagc gtgcttcaca ttccgaatct gaacaggatc aaagcggcag cacccaaaaa
     4561 aatcaaagcg gaacaaccca aaccgcaaac ggcgaaaccc aaagccgaaa ctgcatccat
     4621 gccgtctgaa ccgtccaaac aggcaacggt agagaaaccg atagagaaac ctgtcgaaaa
     4681 accggttgaa aaacctgaag caaaagttgc cgcgcccgaa gcaaaagcgg aaaaaccggc
     4741 cgttcgaccc gaacccaaac ccgcagtgtc cgaaactccg gcttcggcga cagaacgcca
     4801 acccggacct gtacccgctg caaatactgc cgcatcggaa accgctgccg aatccgcccc
     4861 ccaagaagcc gccgcttctg ccatcgacac gccgaccgac gaaaccggta acaccgtttc
     4921 cgaacctgtt gcggaaacag ccagtgaagt tgccgccgaa cccgttgccg aacctgtcga
     4981 acaggtttct gccgaagaag aaaccgaaag cggactgttt gacggtctgt tcggcggttc
     5041 gtacaccttg ctgcttgccg gcggaggcac ggcattgatc gccctgctgc tgcttttacg
     5101 ccttgcccaa tccaaacgcg cgcgccgtac cgaagaatcc gtccctgaag aagagcctga
     5161 ccttgacgac gcggcagacg acggcataaa aatcaccttt gccgaagtcg aaactccggc
     5221 aacgcccgaa cccgctccga aaaacgatgt aaacgacaca cttgccttag gtggggaatc
     5281 tgaagaagag ttatcggcaa aacaaacgtt cgatgtcgaa accgatacgc cttccaaccg
     5341 catcgacttg gatttcgaca gcctggcagc cgcgcaaaac ggcattttgt ccggcgcact
     5401 tacgcaggat gaagaaaccc aaaaacgcgc ggatgccgat tggaacgcca tcgaatccac
     5461 agacagcgtg tacgagcctg agaccttcaa cccgtacaac cctgtcgaaa tcgtcatcga
     5521 cacgcccgaa ccggaatctg tcgcccaaac tgccgaaaac aaaccggaaa ccgtcgatac
     5581 cgatttctac aacaacctgt tctcaaacaa ccatatcggc acagaagaaa cagcttccgc
     5641 aaaacctgcc gcaccctccg gactggcagg cttcctgaag gcttcctcgc ccgaaaccat
     5701 cttggaaaaa acagttgccg aagtccaaac accggaagag ttgcacgatt tcctgaaagt
     5761 gtacgaaacc ggtgccgtcg cggaaactgc gcctgaaacg cccgatttca acgccgccgc
     5821 agacgatttg tccgcattgc ttcaacctgc cgaagcaccg gccgttgagg aaaatgcagc
     5881 ggaaatcact ttggaaacgc ctgattccaa cacctctgag gcagacgctt tgcccgactt
     5941 cctgaaagac ggcgaggagg aaacggtaga ttggagcatc tacctctcgg aagaaaatat
     6001 cccaaataat gcagataccg gtttcccttc ggaatctgta ggttctgacg cgccttccga
     6061 agcgaaatac gaccttgccg aaatgtatct cgaaatcggc gaccgcgatg ccgctgccga
     6121 gacagtgcag aaattgctgg aagaagcgga aggcgacgta ctcaaacgtg cccaagcatt
     6181 ggcgcaggaa ttgggtattt gattcccaac tgccctttcg cagatcaagg atgccgtttc
     6241 agacggcatc tttttttgcc ttatcggtgt aacggataaa gtttgaaccg gcacaggctc
     6301 aaacagcagg ccgacggcaa caaaatgccg tccgaaaccc ctaaaggctt cagacggcat
     6361 tggcggcggc ggtatcaggt attcgcatag gcaaccgaat cgtcgccgct tttcttttga
     6421 acgaactcga ttttgcatcc gtcggggtct tcgacgaagg ctatcacggt tgtgccgtgt
     6481 ttcatcagac cggcttcgcg gacgacgttt ccgccctgcc gcttcacgcg ttcgcaggct
     6541 tcgtaggcat cgtccacttc aacagcgatg tgtccgtagg cgttgcccaa gtcgtatcgt
     6601 tccgtatccc agttgtgcgt cagttccaaa accgtgctgt cggtttcgtc gccgtaaccg
     6661 acgaaggcaa gggtaaatct gccttcggga tagtcttttc ggcggagcag tttcataccc
     6721 aaaacgtttt ggtaggaatc gagggatttt tcgagattgc ccacgcggag catagtatgg
     6781 agtaagcgca tttttcgtgt tcctctcggt ggtggttaaa cttcgatttt attcggcgta
     6841 aacgtctgcc atttgttgca ggcgggacag tgccagaaaa agacttggga tttgaagtgg
     6901 cagttgcggc aacggtacat cacgctgcgc tggagctgcc gtccgataac cgaacgcatc
     6961 atgtcggcat cggctttcca agccggatcc aaatcgctga gtttcaaacc gagcaggcgg
     7021 tacacgccgt taaggtcggg cttgcggcgg acaagctcga cggcggtttg cgcggcttct
     7081 ttctcgccct taagcagcag ggatttctcg tacacgacat tgatcaggtc aagttcggga
     7141 aacgtctgca tatatcctgt cagacggttc aagccttctt caggttttcc ctgcgcggca
     7201 taggcttcgt aaagcttctc gccgaccatg ctcaagtatg catggttttg ctgctcgatg
     7261 gcggcatagg cttcgacggc ggcagggaaa ttgccttgtc ggtgttcaat gtcgcccaaa
     7321 atcatgttgg cgcgggtgca ttttttgttg gcttcgagtg ccttgccgac attgaaacgc
     7381 gcggcatcga aattggactt gaacagcgcg gcttgggcaa gttcgcaata aaactgtgca
     7441 atctcaaact gatatgtctg ttcgtcgtga ctaagaagtt gggcggtttc aaccgctttt
     7501 tcccaatccc tgtcctgctg gtagatattg agcaggtgct gtctggcttc acgcgccatt
     7561 tcaccgtctt gcagccccaa aaaaatctgt tcggcacgat cgaccaaacc cgcgctttgg
     7621 tagttttgcg ccaattcaaa caggacgcgc gcgcgctttt cgccgaccgt atcgggagaa
     7681 tcgagcattg tccggtgtat gttgatggct ttgtcgtttt cgccgcgctg acggtaaagt
     7741 ttgccgaggg tgaggttcaa atcatacgat tgcggccggc cgtcgacgac ttccgccaac
     7801 tcccttgccg cgcgcccgct gttgcggtcg accaaagcgt ccaggctttt ataaaatccc
     7861 gaagggatgc tttttgcctg cttcaatacg gttttcatat ccacgcgggc ggcaaaccag
     7921 cccatcgtga agaagacggg caaaaggata atcggcagca ggataatcca caattcgttg
     7981 tccatatcgg ctttcttaag gctgtttggc agattcggca gcattttgta tcggcggtgc
     8041 agtcaatttc tgtccgctca agcgcgcact tttcttcact tccgcacgca ggcggctgtt
     8101 ttcgccgcgc aaggacagca gccgcccgaa cagggcaaac attccgaaca cgatgccgac
     8161 gacaaacgcg ccgaacaata cgacaatcag cggcagattg acactctgcc ccggaagata
     8221 ggaaaaggta acggcatcca tattaatgac ggcaagcagc aggaagagca gcaggataat
     8281 gattttgatg acggtataga taagtttcat gtttgctccg taaataaaaa cgccgcacgg
     8341 acagcttcgc actctgaacc aagattgcgt tagtttaaac gatttgcacg gtttcggata
     8401 gaatgcggca gcgtgttcgg acggcatacg gagtatgcga ttgccgacaa ttttaccaaa
     8461 tacaccgctc ctttccattt gaaaaataac ggattggaca ccgcatcgac agaaaaaccc
     8521 gccgcgcact tgtcaaaacc ctgtttgcag gcgtatcttt acaaccttca aattcaaacc
     8581 gttcattgaa acatatcaga ataagaaagg ctttacatca tgagcagacc cgtacccgcc
     8641 gtattcggca gcgtttttca cagtcaaatg cccgtcctcg cctaccgcga aggcaaatgg
     8701 cagccgaccg aatggcaatc ttcccaagac cttacccttg cacccggcgc gcatgccctg
     8761 cattacggca gcgaatgctt tgaaggtttg aaggccttcc gtcaggcaga cggtaaaatc
     8821 gtgctgttcc gtccgaccgc caatatcgcg cgtatgcggc aaagtgcgga cattttgcac
     8881 ctgccgcgcc ccgaaaccca agcttatctc gatgctttgg tcgaactggt caaacgtgcc
     8941 gccgacgaaa ttcccgatgc gcctgccgcg ctgtacctgc gcccgacctt aatcggtacc
     9001 gatcccgtta tcggcaaggc cggttctcct tccgaaaccg ccctgctgta tattttggct
     9061 tcccccgtcg gcgactattt caaagtcggc tcgcccgtga aaatcttggt ggaaaccgaa
     9121 cacatccgct gcgccccgca tatgggccgc gtcaaatgcg gcggcaacta cgcttccgcc
     9181 atgcactggt tgctgaaggc gaaagccgaa tacggcgcaa atcaagtcct gttctgcccg
     9241 aacggcgacg ttcaggaaac cggcgcatcc aactttatcc tgattaacgg cgatgaaatc
     9301 attaccaaac cgttgacaga cgaattcctg cacggcgtta cccgcgattc cgtgctgact
     9361 gtcgccaaag atttgggcta taccgtcagc gaacgtaatt tcacggttga cgaactgaaa
     9421 gctgctgtgg aaaacggtgc ggaagcgatt ttgaccggta cggccgccgt catctcgccc
     9481 gttacttcct tcgtcatcgg cggcaaagaa atcgaagtga aaaaccaaga acgcggctat
     9541 gccatccgta aggcgattac cgacatccag tacggtttgg cggaagacaa atacggctgg
     9601 ctggtcgaag tgtgctgatg ctttaaataa aaaaatgccg tctgaaaccc gtttggcgtt
     9661 tcggacggca ttttcgcgtt cggaccgttt ccgccgcacc tgcagcaagt cggtacaaag
     9721 gcaatcggtt aaaacaagcg tccgcatttc ccatcccgcc tgccgcaagt cgggcatttc
     9781 cctggaaata cgcttcaaac ggcaaaccgc cgcccgaaac cgatactcgg cacggacggc
     9841 gcacggattt gaaaacggcg gattatccct cggtgctcaa ggcattgatg ctgtaaccgc
     9901 cgtcaacata agtgatttcg ccggtaatgc cggacgacag gtcggacagc aggaaggcgg
     9961 cagtattccc gacttcttcg atggtaacgt tgcggcgcag cgggttgtgg gcggcgacgt
    10021 gtcccaagag tttgccgaaa tcggcgatgc cggaggcggc gagcgttttg atcgggccgg
    10081 cggaaatgcc gttgcagcgg atgccctctt tacccagaca ggcggcggta aagcggatgc
    10141 ctgcctcaag gctggctttt gccataccca tcacgttgta attcggaatc gcgcgtaccg
    10201 cgcccaagta gctaagggcg acgatggcgg agtttctgcc gcgcatcatc ggacgggcgg
    10261 cttttgccaa tgcgggcagg ctgtatgcgg aaatttcgtg tgcggtgttg aacgcttcgc
    10321 ggctgatgct gtcgaggaag tcgccgctca aggcttcttt cggcgcgaag ccgatggaat
    10381 gcaccaaacc gtccaagcca tcccaatgtt tgcctaagtc ggcgaacact tggttgattt
    10441 cgtcgtcgct ggcgacatcg cagcggaata cgagttcgga atctaattcc gccgccattt
    10501 tgcggacgcg ctcttccagt ttgtccacaa cgtaggtaaa cgccagttcc gcgccctgtt
    10561 cgcggcaggc tttggcgatg ccgtaagcga tggaacgctc ggaaatcatg ccggtaatca
    10621 gaattttttt gccttgcaga aaacccattt tgtatccttc aaatagtgtt cggttcgttc
    10681 taaaccgttg attatagcaa attgtccctg tttctgtgtt ttcacgttgc agcgtgcgga
    10741 cggcaatgcc gtccgaagcg gatttcagac ggcattggac gtttcaaata cggtttaagg
    10801 catcagatgc cgcgcaagag ttcgttcacg ctggttttgg cgcgggtttg cgcgtccacg
    10861 cgtttgacga tgacggcaca gtaaaggctg tggctgccgt ctttggaagg caggctgccg
    10921 gacacgacga ccgaacctgc cggtacgcgg ccttggtaga tttcgcctgt ggtgcggtcg
    10981 aagattttgg tggattgacc gatgaacacg cccatcgaaa tcacgctgcc ttcttcgaca
    11041 atcgcgcctt cgacgatttc ggaacgcgcg ccgatgaagc agttgtcttc aatgatggtg
    11101 ggtgcggcct gcaggggctc gagtacgccg ccgatgccga caccgccgct caagtggacg
    11161 tttttgccga tttgcgcgca agagccgacg gtcgcccaag tatcgaccat cgcgccttcg
    11221 tcgacgtatg cgccgatgtt gacgtaagac ggcatcagca cggcgttttt ggcaacaaag
    11281 ctgccgcgtc gggcaaccgc acccggaact gcgcggaagc ctgcgttttt gaactcgtct
    11341 tcagaccagt cggcaaattt ggtcggcact ttgtcgaagt atttgttcac gccgtcgttg
    11401 aggacttcgt tgtcttggat gcggaaggac agcaacacgg cttttttcgc ccattcgttg
    11461 actttccact cgcccacgcc caagcgttcg gcgacgcgca gtttgcctga atcgagttgg
    11521 cggatggttt ccaacacggc ttctttgact tcgggggcaa cggtggtcgg agtgatgtcc
    11581 gcgcggtttt caaaggcggt ttcgataatg ttttgcaaag acataatgtt tccttggttc
    11641 gagatatttc gggacgcttg ccggtattgt tcagacggca tggcggttgg aacaaacgtg
    11701 ctattgtgcc atacaaacgc ccgcccgtca gccgcgccgc aggcttgccg gatgccggcg
    11761 tttcggaatc gggcgggcgg caggcatatg tctttttccg cgcccgaatc aatacagaaa
    11821 ggcggcggta cttttatgcc gcgccgccgc ctttcagacg gcattcgcgg taaaacgcca
    11881 tcagcccttc ggtcgaggca tcgtgtacgg acgtgccgcc ttccagttcg ccgatgatgg
    11941 tttttgccaa ctgtttgccg tactcaaccc cccactgatc gaaggggttg acgttccata
    12001 tcgcgccttg gacgaaggtt ttgtgttcgt aagccgccat cagcataccc agattgtagg
    12061 gcgtgaggcg gtcgagcagg atgctgttgc tggggcggtt gccggggaac tctttgtgcg
    12121 gcgcgaggcg ttcgcgttcc gcttcgggca aatctgccag ttcggcgcgt gcttcgtcta
    12181 aggttttacc cttcatcagg gcttccgctt gggcaaaggc gttggcaacg gtaaaacggc
    12241 tgcgtccgtc ctccgcgccc tgcgccgtca tcgggacgat aaagtcgcag ggaatcaggc
    12301 gcgtgccttg gtggagcagt tggaaatagg cgtgctggca gttgacccct tcaccgccga
    12361 acacgatgcc gcccgttttg cacgcggcgg gactgccgtc tgaagcgcgg cttttgccca
    12421 aactctccat atcgagctgg ttcagccacg ccggcagcag gcgcaggttg tggctgtacg
    12481 gaacggcggt ctgcccgtcc gcgtgctgga aattgttata ccacacggca atcagtgcca
    12541 tcagaacggg aatgttgcga cgcggcggcg tgtggaaaaa atggctgtcc atcgcgtgcg
    12601 cccccgctag cagttcgcgg aaacgcgccc cgccaaccgc aaccatcacg ggcaaaccga
    12661 cgggcgacca gacggaatag cgtccgccca cccagtcgta catcgcaaac acgcgttccg
    12721 ccgcaatgcc gaaactttgc gccgcttccg tatcggcaga caccgcgcaa aaatggcgcg
    12781 ccgtttcgga ttccgagaac cccgcaccgc gataccacgc cttgaccgcc tctgcattga
    12841 gcagggtttc cggtgtttta aaggacttgc tggcaacgca aaacattgtt gtttcggggt
    12901 tcagacggca taagacctca tccaggcagg cagggtcggc attagaaaca aaatggacgg
    12961 aaatctgccg tctgaacggc tcgagcgcct gcacgcacat tgccggcccg aggtcggatc
    13021 cgcctatgcc gatgtggaca aaatccgcaa tccgttttcc ggttatcccc tgatataaac
    13081 cgtcgtccaa actgtgtgca aacttcaacg cacgatttaa ctcgcggcgg atttcgggca
    13141 acacgtccct gccgtccgca taaacggcat ccgcaccgtc gggcaggcgc aaagccgtat
    13201 gcagcgcggc acgcccctcg ctgccgttga ctttcgcacc cgtccgcaaa gcacgcattt
    13261 tcccttccaa atccgccgtc tccgcaagac ggcagagcag ttgcagcgta tcttcgccaa
    13321 aacggttttt gctgtaatcg aacaacatcc cgtccaaacg ctcatgcata cgctcaaaac
    13381 ggtcgggttc ggcagcaaag cggtcgcgca aaaggatgtg gcacgtatcc tgataatggc
    13441 gttcgagcgc ataccatgca cgggtaaagg catccatctg ttttccttga tttttcagaa
    13501 ccggattaaa atatagcaga atgtagtcta acaaacggca gacactttag cgaatctcag
    13561 gaacaccgcc cgccgcgtcc aataacacct tatttccata tggcgaaaat atagtgaatt
    13621 aacaaaaatc aggacaaggc ggcgggccgc aggcagtacg gatagtacgg aaccggttcg
    13681 cttggtgctt catcacctta gggaatcgtc ctctttgagc cggggcgggc caacgccgta
    13741 ccggtttttg ttaattcact ataccctccg gccggcattt gacccgcaac aatatcctgc
    13801 aagatttatt gtgtacgcat aaatgccgga cagccgccta aattaaggta taatcgcatc
    13861 cgattctgtc ccgtttgccg cccgcaggcg ggatgtcggc ggttttcatc ttaccgggaa
    13921 cacgccgata ctgtttttta acgatgtttc ttacatttta ttccaattac tttacggggc
    13981 tagaatatgg ctaaaaatgg gggattttct ttgttcgcaa agaaagaaaa acgctttatc
    14041 tttgaaggca ggcattctgc ctccgacaaa ctggtcaacg gcgaagtatc cgcgtttacc
    14101 gaagaagagg cacgcaaaaa actggcaaaa cgcggcatcc gcccgttgca gattacccgt
    14161 gtgaaaacaa gctccaagcg caaaatcaca caagaagaca ttaccgtttt cacccgccag
    14221 ctttccacga tgattaaagc gggcctgccg ctgatgcagg catttgaaat cgtggcgcgc
    14281 ggacacggca acccgtctat gacggaaatg ctgatggaaa tccgaggcca agtggaacag
    14341 ggcagctcgt tgagccgcgc attctcaaac cacccgaaat atttcgaccg cttctactgc
    14401 aacctggttg cggcgggcga aacgggcggt gtattggaaa gcctgctgga caaattggca
    14461 atttacaagg aaaaaaccca ggccatccgc aaaaaggtaa aaaccgcgct aacctatccg
    14521 gtatccgtca tcgccgtcgc catcggtttg gtattcgtga tgatgatttt cgtactgccc
    14581 gcctttaaag aagtttacgc caatatgggc gcggagcttc ccccactgac ccaaacagtg
    14641 atggatatgt ccgacttttt cgtctcatac ggctggatgg tgctgatcgc actgggcttt
    14701 gccatatacg gcttccttaa attgaaggcg cgttcgatta aaatccaacg gcgtatggat
    14761 gccatactgc tgcgtatgcc gattttcgga gacattgtcc gcaaagggac gattgcccgc
    14821 tggggcagga cgacggcaac gctgtttgcc gccggcgtgc ctttggtcga tgtattggac
    14881 tccactgccg gcgcggcggg caatttaatc tatgaagaag ccacccggga aatccgtacg
    14941 cgcgtcatcc aaggtctgtc tatgacttcg gggatgcgtg cgacggaact gttccccaat
    15001 atgatgttgc agatgtcctc catcggcgag gaatcgggtt ctttggacga tatgctcaac
    15061 aaagccgccg aattttacga agacgaggtg gacaatgcgg tcggcaggct gtccgctatg
    15121 atggagccga ttattattgt gattttgggc ttggtcatcg gtacgcttct ggtcgctatg
    15181 tatctgccgc tgttcaactt gggcaacgtg gtcgcctgat ttgccgcaca gatccggcgc
    15241 ggattggttc tgcgccggtt tgtttttgct ttgaatatat caaggacaaa atatgtctga
    15301 tttgtctgta ttgtcgccgt ttgccgtgcc tttggcagca gttttggggc tgctggtcgg
    15361 cagcttcctg aatgtcgtca tttaccgcgt acccgttatg atggaacgcg gctggacggt
    15421 atttgccaaa gaacatttaa acctgccgct gaccgacgat gaaagccgta ccttcaacct
    15481 gatgaagccg gattcctgct gtcccaaatg ccgtgtgccg atacgcgcgt ggcagaacat
    15541 cccgattgtc agttacctgc tcctgcgcgg caaatgcgct tcctgccaaa ccaaaatcag
    15601 catacgttat cccttaatcg agctgctgac cggcgtattg ttcgggctgg tcgcctggca
    15661 atacggctgg tcttggatta cgctgggcgg tttgatactg accgcgtttc tgatttccct
    15721 gacctttatc gatgcggaca cccaatacct gcccgactcg atgacattac ccttgatctg
    15781 gctggggctg atatttaatt tggacggcgg cttcgtgcct ttgcagtctg ccgttttagg
    15841 tgcggttgcc ggctatagtt cattatggct cttatgtgca gtgtataaac tgctcacagg
    15901 aaaaaccggt atgggcaacg gagatttcaa actgattgcc gcattgggcg cgtggctcgg
    15961 catatccgca ttgcccgtgc tgatttttgt ttcctctctg atcggtttgg tcgcggcaat
    16021 cgttatgcgc gtcgccaagg ggcggcattt tgccttcggc cccgcactga cagtttcggg
    16081 ctggataatt tttacggcaa acgattccgt atggcgggcg gtcaactggt ggctgaccca
    16141 tccggtgaga tgacagcatg ggtcggactg accggcggaa tcggcagcgg caaatcggca
    16201 gccgcgcaat attttgccga tttgggcgtg ccgcgcatcg atgcggacgc ggcggcgcac
    16261 tcgctgacgg cttcagacgg catcgccctg ccggaaatca ggcggctgtt cggcgacacc
    16321 gttttcgaca cacagggttt gttgcggcgc gacatattgc gtaaagaaat ctttgcctcc
    16381 ccatcgcgca aagccttgct cgaatccgtg atgttgccgc tgattttctc agaaatcaaa
    16441 aaacagcaag aaacctttac cgatgcggtt tacggcattg tcgaaattcc gctgctgacg
    16501 gagaagcgtc aatttatcag cctgatacgg cgtgtcctga ccataagcgc acctttggaa
    16561 aaacgtatcg gcagggtaat ggcacgcagc gggctgacgc gcggcgaggt cgcggacatc
    16621 atcagccatc aggcatccga atccgaacgc ctgctgcttg cagacgatgt actgctcaat
    16681 gacggcagcc tcaaaagcct acgtgagaaa acaatgctcc tgcacgcgtt ttattcaggg
    16741 attttcgcct caaaaccaac acaaggaaaa cacaatggct gaatcgcggc aaacacgcct
    16801 tcaagtcaaa tgtccgacct gtcaaacggc agtagtatgg aaaccggaaa acgcattccg
    16861 cccgttctgt tcgcaacgct gcaaactgat cgacttgggc ggatgggcag acgggaaata
    16921 tacggtttcc ggccaaacgg aaagtttgcc ggaaatatcc gaacccgacg gggcataccg
    16981 ctgaccgccc cgccttcccg gcaaacaccc tgaaaggtca aatgccgtct gaaacaaaca
    17041 cgcttcagac ggcattttca ttctcaaacc taatcgttgg tatttgccgt tacctcttcc
    17101 aatgaagtaa tgccctgcat aattttcaaa ataccggccc ggcgcaaatc caccataccc
    17161 tccttatagg caacgtccaa aatacccact tccgtaccgt tgttcataat cacacgctgc
    17221 atttcttcgc tgatgggcat aacctcatac acgcccgcac gccccttata accctgcccc
    17281 cggcaacggt cgcaaccgac ggcgcggtaa agtttccaat cttttgcaag atcctcatcg
    17341 gtgaaaccga cttccttcaa agcagaggca gacgggcgtt ccacttcctg tttgcagctc
    17401 gaacacagcc tgcgtaaaag acgctgcgcc ataatcaggc tgaccgaact ggcaatatta
    17461 aacggcgcga cacccatatt cagcatacgc gacaacgtcg ccggcgcatt attcgtgtgc
    17521 agtgtggaaa acaccatatg ccctgtttgt gccgccttaa tcgcaatatc ggcagtttcc
    17581 aaatcacgaa tctcaccgac cataatgatg tccgggtcct gacgcaggaa agacttcaaa
    17641 gcagcggcaa aagtcagacc ctgcttatca ttgacgttaa cctgattgat gcccggcagg
    17701 ttaatctcgg cagggtcttc cgccgttgca atatttaccg actccgtatt caaaatattc
    17761 aaacaggtat agagcgacac cgtcttaccc gaacccgtcg ggccggttac cagcaccatc
    17821 ccgtaaggac ggtgaatcgc ttccaacaac aattttttct ggaacggctc aaaaccgagc
    17881 tggtcgatgt tcaaagacgc ggcatcggaa ttcaaaatcc gcatcacgac cttttcgcca
    17941 aacagcgtcg gcaatgtgct gacacggaaa tcgacaggct tgccgccctt ttgaaaggtc
    18001 agctgcatcc taccgtcctg cggtatccgt ttttcggaaa tgtccaaacg cgacattacc
    18061 ttaatccggg aagcaagctg cccccttacc gcaatgggcg gctgaaccac ctcgcggagc
    18121 tgcccgtcca cacggaaacg gatacgcgca ttgtgttcgt aaaactcgaa atggatgtcg
    18181 gatgccccgc tacgcaaggc atccgacaaa gttttatgga taaacctcgg aacagggccg
    18241 tcttctgcct cctcgttgtc gatatacagg gtgtggcttt cctcttcctc ttgcccctcc
    18301 ccaagctcct gaagcagcga tgtcgaacgc gaacccaccc aatcgagcaa acccgccaac
    18361 tggtcatcct cgacaatgac caactcaacc gcaatccctg cggcagaaac ggttttctga
    18421 atttgcggca tctgggtcgg atcggaaacc gcaaaaaata ctttgtcgcc ccgacggaaa
    18481 accggcacac agtggaactc caccatctgc tcctccgtca acacccccat cagcaccctg
    18541 tggcgcggat aatgacgcaa atcaagaatc gaataactga acaccctcgc aatcaatgcc
    18601 gcaagcgact tgggcgaaat gacaccgtct gaaaacagca tcggcaacac ttccttaccc
    18661 gcctgcgact cattgtagta atgctcggcc tgctcaacag taaccacctg gttttgaacc
    18721 agaatcctca gcaaacctac gctcatacga ccttatcccc aaatttattc attgttatac
    18781 ctgtacagat tttatcataa aacaacagat ttccaagcct gaacatcttt tacgaagcct
    18841 gaaaatcatt tcacgattct gccgtcctaa aggtcggttt ttcaagcagg aagaaaaagt
    18901 tttcagatgg caaaaaagcc ctccagcact gaaaggcctt atatcggaaa cttcccgcaa
    18961 cacgggaaac agacaaatgc aaaccgtcaa acctcgccaa caggaatcga acctgtattt
    19021 tacgcttagg aggcatacgt tctatccgtt gaactatggc gagccgaaat gaaaagggag
    19081 attttaaacc tttccggcaa caaaagacaa gatgtcggcc gcaatcagcg gcccgacagg
    19141 gataccgccc ataaatgcca ccccgatgac cgtaccgatc agcaagcccg ttaccaatac
    19201 cggctgccgt cccatcaaag gcacgccgca tcccgccagc caagccacga aaataccgat
    19261 aaaaacggcg gatatcattt taaaattcaa aaattcggca acaggaggaa cctgcgcctt
    19321 tcctgaaacc aacggactca aaacccctat ggtcaaaaga atgataccga ggttcaaccc
    19381 gtgcttctcg accaacggga caaactgtac caatgccgtc tgctgcatca gcagcaatat
    19441 ggttgccgag acggtaatcg aattgttgtt gctgaccacc cccaacagaa tcagcgtaac
    19501 cagaaacagg ggaacaaaac tgaaattcat cggttacgac accctgacca aagattcctt
    19561 cgccagctga atcatagcat ccttcacttc gctgtcggac agggcatcca acgcggcaac
    19621 ggcacaatcg actgctttgc gcgcctcgcc tatcgaatat gccaaagcat ccgaacggac
    19681 gacataatcg cggatttttt caaaatagcc gcgatctgca ttttccaaag cagtacgcac
    19741 atcgttcgca acctgttcgg aaccctgacg catcagataa atcaaaggca gggtcggttt
    19801 tccttccgcc aaatcgtcgc cgacgttttt acctgtttct tcggtttccc ccgaataatc
    19861 cagcacatcg tcaataatct ggaatgccgt accgacgtac ataccgtagt ctttcaaggc
    19921 ctgttcgtgc ccgggggaag ctttgcccaa aattgcgccg acttgagcgg cagcttcaaa
    19981 caattttgcc gttttatatt ggatgactcg gatatattgt tcttcggtaa tgtccgtatt
    20041 gccgatgttc atcagctgca tgacctctcc ctcggcaatg atgttggtcg catccgccat
    20101 cacttccaaa acgcgcatac tgcccgaggc aaccatcagt tgaaatgcac gggtatataa
    20161 aaagtcgcca accaacactg ccgccgcatt gccgaacaga ttgtttgccg ttgcccgccc
    20221 acggcgcaaa tcgctttcat cgacgacatc gtcgtgcagg agtgtggaag tgtggataaa
    20281 ctcgaccatc gctgccagcg aatacagttt ctcgtcatca taaccgaccg ccttacccgc
    20341 caaaatcgtc ataatcggac gcaggcgttt gccgcccgcg ctgatgatat atgtaccgat
    20401 ttgcgaaatc agtgcgacat cggattgcac cgcacggttg atgacttcat tgactttggc
    20461 aaggtcttca ggcagatgtc gctggaaata gggcagattc tcgagcatgg cagactctca
    20521 ggtttacgtt tccgtcaaac ggcagatacc gcttgaaagc tgattcaaat agcgggcaat
    20581 tatatcagca aagccatcag ggtacatctt ttcagacggc atatgattgc ccgaaccgcg
    20641 caaactttga caaaacaggc aaataacaat acaatgccca atttcgcaca atggtgtgcg
    20701 gatattaacc ataaccctta tggagttgag tatgtacgcg gtcgtaaaaa ccggcggcaa
    20761 acagtataaa gtttccgtcg gcgaaaaatt gaaagtagaa cagataccag cccaactcga
    20821 cagccaaatc gaactgaccg aagttttgat gattgctgac ggcgaatctg taaaagttgg
    20881 cgcacccttt atcgaaggtg caaaagtaac ggctaaagta gtggcacacg gtcgtggcga
    20941 aaaagtccgc atcttcaaaa tgcgccgccg caaacactac caaaaacgcc aaggccaccg
    21001 ccaaaatttc acccaaatcg aaatcgtggc aatcgcctaa ccttaaaact taggagcatt
    21061 acaaatggca agtaaaaaag caggcggcag cacccgcaac ggtcgcgatt cagaagccaa
    21121 acgcttgggc gttaaagcct acggcaacga gctgattccc gcaggttcca tcatcgtacg
    21181 ccaacgcggt accaaattcc acgcaggcga caacgtaggt atgggcaaag accacacttt
    21241 gttcgccaaa attgacggtt atgtcgaatt caaaaccaaa ggcgcgctga accgtaaaac
    21301 tgtcagcatc cgtccttaca ccggttctga agaataattc aaccgatacc tgaagccgca
    21361 tcttttcacg atgcggcttt ttacataccc catacccgat ttgatacaca ttaagctgaa
    21421 agtaaaaatc cgcatacacc ctcccctgca tatttcttca acaacgggtt ttgatataat
    21481 cgcctatctg ttacagatag ttcaaacggc attcaaaccc ttacaaatgc cgtctgaaat
    21541 catcggcatc cgcctatgca aaggatattt tatgagttta cacagcgaca tcctcgtcgt
    21601 cggcgcaggc ccggcgggtt taagttttgc agccgaactt gccggcagcg gtttgaaagt
    21661 tactctgatc gaacgcagcc cactgactgt tctgcaaaat cctccctatg acggacgcga
    21721 aatcgccctg acccattttt cccgcgaaat catgcagcgt ttgggtatgt gggacaaaat
    21781 tcccgaaaac gaaatctacc ccttgcgcga tgccaaagtg ctgaacggac gttccgacta
    21841 ccagctccac tttccccaac cgactgaagc gcgcggcgag cctgccgact gtttgggcta
    21901 tctgatttcc aaccacaata tccgccgcgc cgcctatgaa gtcgtatcac aactcgacaa
    21961 tgtcagcatt ctgaccgata cggccgttaa agaagtcaaa acatctgata atgaagcgca
    22021 agtctttttg gaaaatggag acattctaac cgcgcgtctc cttttggcag cagacagccg
    22081 tttctcacaa acccgccgac aactcggtat ttcttcagat atgcacgact acagccgaac
    22141 catgttcgtc tgccgcatga aacataccct ttccaaccaa cataccgctt acgaatgctt
    22201 ccattacgga cgcaccatcg cactgctgcc actggaaaag cgcctgacca ataccgtcat
    22261 tacagtcgat accgataaaa tcaacagcgt tcaaaacctt tcgcccgaag aattggcggc
    22321 aagcgtaaaa gaacagctca agggcaggct cggcgacatg gaactggtca gcagcatcca
    22381 ccattacccc cttgtcggca tgattgccaa acgtttctac ggcaaacgca gcgcgctgat
    22441 cggcgatgcg gctgtcggta tgcaccccgt aacggcacac ggcttcaacc tcgggctttc
    22501 cagcgcggat attctggcaa aactgatact cgaagccgaa cagcgcggtc aagacatcgg
    22561 cgcggcttcc ttgttggaaa aatacagcag caaacatatg ctgcacgccc atccgcttta
    22621 tcacggcacc aatatgatgc tgaaactgtt taccaacgaa accgcgccgg caaaactgtt
    22681 gcgcggtttg gtattacgag ccggcaacaa cttcccgccg ctgaaaaagc tgattaccaa
    22741 acaactgacc ggttgaaaac tgccgaaatg ccgtctgaaa cgtttcagac ggcatttttg
    22801 tattatgaac cgttttgcgg tttccatatc cttggcagac ggtttgcagc agattcgcat
    22861 acggcagatg tttcaagcag aaaggaaaca caataaaaat aaaaaagcct ccgaacagtc
    22921 ggaggctttg ctttcaaact gaaattattt cagtttggtt tctttgtaca ctacgtgttt
    22981 gcgggcaacc ggatcgaatt ttttgatttc cagtttgccg ggcatagtgc gtttattttt
    23041 ggtagtggtg tagaagtggc cagtacctgc accggattcc agtttgattt tatcgcgcat
    23101 tgcagtaatc cttaattaaa tagtgtttaa attaagcttc gccgcgagca cgcaaatcag
    23161 ccaatacgac atcaatgcct actttgtcga tggtacgcaa tgcagcgttg gaaacgcgca
    23221 ggcgaaccca gcggttttca ctttctaccc aaaaacgacg tgattgcaag ttgggcaaaa
    23281 aacggcgttt ggttttgttg ttggcgtgcg atacgttgtt gccggacatc gggcgtttac
    23341 cggtcacttt gcaaactcgt gccataatca gtctccaaac ttctaaaata gtaaaacgcg
    23401 atttatacca cgaaaaacaa tgccgtttca agtgttttaa agaaaaacag atgttttgcg
    23461 cgggtaagcc gcccgagtgc cgcatctgcg gaacgtttgc aggtgcgaaa cagatggggc
    23521 gcgattatag cggatttcgg gagcttctgc attcaaaatg ccgtccgaac atatttccag
    23581 tattttcaga cggcatttca agttttcaaa tccctcaatg tccaccgccg ccgctgtgga
    23641 acggcggttt cgccagccag acaatgggaa tcaggacgat aaacaaaatg ctgccggcga
    23701 ggaatatttc gttcgatccg atgatgaagc cctgttgcgt aatggtattg ttgatgatgc
    23761 ctagagtttg accgtcggaa atgccttgtt gggacaaatg cgccgccgtt tcgtgcaatg
    23821 ttgcggaata gggcgtgatg tgttcggcaa aacgcgtgtg gtgcaacgct tcgcgccgtt
    23881 cccacagggt gctgacgacg gatacgccga caccgcccat cagcacgcgc aggaaattcg
    23941 acaggctgcc ggcggcggca atctgtccgc ccttcatgtg cgacagtgtg atggtggtca
    24001 gcggcaggaa gaacatggca acgcctaccc cctgccaaaa ctgcggccag atgacgttgc
    24061 cgatatccat gtcggcataa aaatcagtgc gccaataaaa agtgaaggca aaggtcagga
    24121 agctggcggt tacgagcagg cgcatatcga ttttattgcc gaacctgccg attaacggag
    24181 ataggaagac ggggaggatg ccgacgggcg cggcagcaag ccctgcccac gcggaggtat
    24241 agcccaagtt ggtctgcaac actaacggca gcagggtcag cgtccccata tacaccatga
    24301 aacccaacga ggtggcaatt acgccgacgg taaaattccg atctttaaac agcgataaat
    24361 cgacaatcgg atatttttct cccaattccc aaacaataaa atacgacaag cacaccagcg
    24421 cggttatgcc caaggtaatg atttctccag aggcgaacca gtcaagttcc ttacccctgt
    24481 ccagcatcat ctgtaacgcg ccgataccga ccatcatcag cgtcagcccg acatagtcgg
    24541 tcggcgttct gacggttgcc gtttcgcggt gtttcaaatg tttccatgta atccatgccg
    24601 atatgatccc gatggggata ttaatgaaga aaatccaacc ccaatgccag tttccggaaa
    24661 tccaaccgcc gagtatcggc ccgaggacag gggcgacaac gacggtcatt gcccacaatg
    24721 ccagtgccag catccgtttt gcgggcggat aggatgccat taacaggctt tgcgacaggg
    24781 gaatcaacgg cccggcgata aagccctgca aaatgcggaa aacaaccagc gactgaaggt
    24841 tgggcgcgat gccgcacagc cacgatgcga tgacgaaacc ggcggcggcg gcggtaaaca
    24901 atttgacctc gccgatacgt tttgccaaaa agcccgtcag cggcacggaa acggcgtttg
    24961 ccacggaaaa agaagtgatg acccacgtcc cctgcgtggt tgccgcaccg aggttgccgg
    25021 caatgacggg gacggcgacg ttggcgatgg tcgtatctaa aacctccata aatacggcaa
    25081 gccccaaaga cagcgtaacc cacgccaatg ccgcaccctt aagcggtgga taatccattg
    25141 tgtcctccgt ttcagacggc atcagggcgc ggattgcccg aggatttcat cgaccgtccg
    25201 atcgacttcc gaccagtcgg tactttccat ttccggcaat gccgcacccg gcgtttttga
    25261 aacaggcgcg cctgcggcgg aagtatccac tttaaccgtc atcgacaaac cgatacgcaa
    25321 cggatgcctg tccacatctt cgcggttcag cacgatacgg acggggacgc gctgcaccac
    25381 tttaatccag ttgcccgttg cgttttgcgc cggaatcagc gaaaacgcgc tgcccgtacc
    25441 tgccgaaaac cctgccaccc tgccgcgata aacaatttgt ttgccgtaca aatcggacac
    25501 cagctcggca ggctgtccga ttttcatatg ccgcaactgc gtctctttaa aattggcatc
    25561 cacccacaca tccgacagcg gcaccaccgc catcagcggc gcgcctgccg ccacctgctg
    25621 cccgacctgc accgaacgct tcgccacctg accgtccgcc ggcgcgcgga cttgcgtccg
    25681 ccgaaggttc agccacgcat ctttcaacct gccgattgcc gtctgaacct ccggctgttc
    25741 gcgcaaagaa acacctccgc ccaaagccgc gcgtgccgaa gattcttccg ccaaagccgc
    25801 cttaaccgcc gcctgcgcct gagacactgc cgtacgggcg tgtgccagct cttcggcgga
    25861 caccgcaccc gattccgcca aagcggaccg gcggcgcaaa tcatcctgcg cgcgcgccaa
    25921 atccgcacgg cgcaaggcaa cctgcgcccc cgcctgcgaa gtggcggcat tttgccggcg
    25981 gttttggcgc accgcctgaa ccaactcgtt ttttgcccgc tcgtaagcca gcacatcatt
    26041 atcgtcatcc aacaccgcca gcacgtcgcc ttttttcacg gcatccgtat cgtcgtgcaa
    26101 aaccttccgc accgtgccgc ccttttgcgg cgtaacctga accacgcgtc cggcaacata
    26161 agcgtcttcc gtttcctctt cgtgctgcca ccataaaaaa aacgccgatc cggcggctgc
    26221 ggcggaaagc gcgaacagca gcgtcaatgc cgtcaggcgg cgtttgcgtt tgacttgcgt
    26281 gttttgaagt tttgtttcgt ctgtgtgcgt ttccataaga ttgggttccg aatttcctga
    26341 tttcatataa acaatgttct ttcaatagga aatagatttt atatccgcgc aataacgcaa
    26401 tcaagtcagt attttagatg caatattcaa aaaacaacgc ttcaggtggc aaaactgaaa
    26461 atatatttcg tttatatatg acatggcaaa caccgccgcc cgcaccatgc cgtctgaaga
    26521 aaactacaca aataccgccg cttatattac aatcgccgcc ccgtggttcg aaaacctccc
    26581 acactaaaaa actaaggaaa ccctatgtcc cgcaacaacg aagagctgca aggcatctct
    26641 cttttgggta atcaaaaaac ccaatatccg accggctacg cgcctgaaat tctcgaagca
    26701 ttcgacaaca aacatcccga caacgactat ttcgtcaaat tcgtctgccc cgaattcacc
    26761 agcctctgcc cgatgaccgg gcagcccgac ttcgccacca tcgtcatccg ctatatcccg
    26821 cacatcaaaa tggtggaaag caaatccctg aaactctacc tcttcagctt ccgcaaccac
    26881 ggcgattttc atgaagactg cgtcaacatc atcatgaaag acctcatcgc cctgatggat
    26941 ccgaaataca tcgaagtgtt cggcgagttc acaccgcgcg gcggcatcgc cgttcatccg
    27001 tttgccaact acggcaaagc aggcacagag tttgaagcat tggcacgcaa acgcctgttc
    27061 gagcacgacg cacaataaac catccttccc atatttcaga cggcattcct tcgatacggt
    27121 atgccgtctg aacgcctttc cgaaaggttt ttatgtacgc attgaccgcc gcacagcaac
    27181 agaaggcact cttccggctg gtgcttttcc atatcctcat catcgccgcc agcaactatc
    27241 tggtgcagtt ccccttccgg attttcggca tccacaccac ttggggcgcg ttttcctttc
    27301 ccttcatctt cctcgccacc gacctgaccg tccgcatttt cggttcgcac ttggcgcggc
    27361 ggattatctt ttgggtgatg ttccccgccc ttttgctttc ctacgtcttt tccgttttgt
    27421 tccacaacgg cagttggacg ggcttgggcg cgctgtccca attcaacacc tttgtcggac
    27481 gcatcgcgct ggcaagtttt gccgcctacg cgctcggaca aatccttgat attttcgtat
    27541 tcgacaaatt acgccgtctg aaagcgtggt ggattgcccc ggccgcatca accgtcatcg
    27601 gcaatgcact ggacacgtta gtattttttg ccgttgcctt ttacgcaagc agcgatgaat
    27661 ttatggcggc aaactggcag ggcatcgctt ttgtcgatta cctgttcaaa cttaccgtct
    27721 gcaccctctt cttcctgccc gcctacggcg tgatactgaa tctgctgacg aaaaaactga
    27781 cggccctgca aaccaaacag gcgcaagacc gccccgtgcc ctcgctgcaa aatccgtaaa
    27841 acagctctgt agggaatcgg gacggttggg ttttctttta tttgcaaaca ctgccggaac
    27901 ggataaatcc ggattcccac ccacttcgtc attcccgcga aagcgggaat ccggtgcgtt
    27961 ggggttcagt catttccgat aaactcctgc cgcattgtat ttctagattc ccgctttcgc
    28021 gggaatgacg gcggatgggt ttccgtgccg tggtgtccgg attcccgccg gtacgggaat
    28081 gacggcagcg cttccttggc ttagttttaa tccactataa atgccgtctg aaaacctgtt
    28141 tgcagatttt cagacggcat tgttttcgtc gtcggttcaa acttcaatcc gattgcgaca
    28201 cggtaaccgg tatgccgcgc aagcgcgcaa gcagcgcgtc cgccttctgt ttctgcgcgg
    28261 caaacgccgc cttgtccgcc tgcgtcaatt ccggtgtcgg caatgcgacc gaaacaggat
    28321 tgacgggctg cccgttgatg cgcgcctcgt aatgcaggtg cggcccggtc gaacgccctg
    28381 tcgaaccgac aaaaccgatg acctcgccgc cgcgcacatt gccttgtgcc tgcgaaaacg
    28441 cgctcaagtg cgcgtacagc gtttccacac cgttggcgtg gcgtatcatc accgcgttgc
    28501 cgtatccgcc cttccggcct ttaaaggtaa tcacgccgtc ggcggaagcc ctgaccggcg
    28561 ttccctgcgg tgcggcataa tcgatgcccg tgtgcagccg ccatgtgtgc aggatggggt
    28621 gcatacggta gccgaacggc gaagaaatgc gcgtatagac cagcggctcg atgttgaagc
    28681 cgcctttttc ctgcaacacc ctgccgtctt catcgtaata attgccgccc cctccgcctt
    28741 ccttgtccga acggtaatag aacgcctgat gggttgtgcc gcccttgaca acttccgccg
    28801 ccaaaatatc gcccgccgcc acctgctgcc cgtggaaata caggctgtcg taaagcaggc
    28861 gcacggcatc gccttccttc aaaccgtcaa ggctgaagcg gccggcaaaa atcccgctta
    28921 aggattcgcg gatttcgacg ggcacttccg cccgcgccag cgaaccgcgc gccgacgttt
    28981 tgaccacgac cgaacgcagc gtcggcaaaa ccttcatatc cgcatcagaa gccgaccgcc
    29041 gccatatgcc gccttttttt tccaaagcga ccagattgcg ctcgccgtct tcgtcggtaa
    29101 aaaactgcac ttcgcgcgca ctgccgtcgc cgccgaccaa aacatgaacc gactggtcgg
    29161 cacgcaaatg ccgcaaatcg gcttcgccgc catatttttc cgtgattcgg gcaatctcgt
    29221 cccgcgccat acccgaacgc gccagcacgt ccgccagcga gtcgcccggc tgcaccgcct
    29281 cctgcaccca atatgccgtc tgaacgccgc tgccgcccca agacagcggc ggcagttttt
    29341 gttccacgcg ctgcggtctg acgcgctccg tcccctctgt cgaagcaatg tatgccgccg
    29401 acaccagaat aatcgaaacg gcaagcgcgc gcagcgcgta tttccgatgt tttgccgaaa
    29461 gtgggaagac agccatattg ttcctttttg acggcgtttg cgccgaatcc tctataatat
    29521 ccgcccgatc ctcatttgtc aggcatcaaa cgatgaaact caccctcgcc ctcccctcgc
    29581 tcaatttgga tgaagacgaa atccgaatac ctctatgcct gcccgccttc aacaaaatcc
    29641 tgcaatacgg ctcgccgcac cgccaaagct gcacggcttc ggcattttac gcacgttatt
    29701 tgtggtgcgg aagactggca caacgcccgg cgcaaagcct gaatatgccg tctgaaaccg
    29761 tcgcgctcgc cacgcccgtc tggcaaaaaa tggggctgca tcaggcaaat gtgctgacgg
    29821 cggaatattt ggatgtcggt acggatgaag cggaacggct ctgccgcgat ctgtcggcgt
    29881 tttacggcga cattccgtgg cgtttcgttc ccgtcctgcc cgaattatgg ctggtttccc
    29941 ttccccgcgc gtaccgctgg ggcgcgaagc ccgttttgga cttgggcgga ctgttgggcg
    30001 cggacgatca gccggacggg gaagacgcgt tggaatggct gcgcgtccaa accgaaatcc
    30061 aaatgtggct gaacgctcac cccgtcaacc acaaccgcaa aaaacgcggg ctgcccgaat
    30121 tgaacggctt atggctttgg gacagtctgc acggaagcgc acaaggcggc acgcttttcg
    30181 ccgatacggt ttggagccgt tttcacccga accgccgagc attgcccgac agcttccgag
    30241 cttatgcgga aacggcggca cacctgcccg atacgcacca catcctgttt atggacgatt
    30301 tgcgcctgac cgccctgaca ggggacaggg agcggtatgc ggcaatattg cagcagtggg
    30361 gagaacgctg gttcgcaccg ctttacgaag ccgtccgcac cggcaaaatc aaacggctgg
    30421 acatcgccac ggacggacaa cacggcggta cgctgacctt caaacccaca gacaggcgga
    30481 agttttggcg ctgcacgaaa acctttgacg gaatctggta aacccgcccg ccgcaaccgc
    30541 gacaatgccg tctgaagccc gaatcgggtt tcagacggca ttttaccccg catcgccgtt
    30601 atgccggaaa aaccggaata cgtttccgca cacgcggcgg cacgcaaaac accgcccgaa
    30661 agccgccgat gcccgccgtg acaccgccgc cggcagggtt tccgccgaaa aggaacagca
    30721 atcagaacgt tttgttaaac tcgacaaacg ccctgttttt ctcgtattcg ttaaacacat
    30781 cgttgctcca cgtttcgcgg tgcgacagcg tcaggcgcgg cgtgatgcct ttgaaatgca
    30841 atgcccggtg ccaaaggctc aaggatgtgt ccgattcttt atccctgcgc ctttcccctt
    30901 taaaactgct gaagaagccg ggtttttcat aatgccgttt cgccacgccg aggcggaaca
    30961 gcgaagacag gccgctgccg ccccattcct gcccccaggc aaagcgcagg ccgtaacggt
    31021 tgaaattgtc gccacggtcg gcggggttgc gctcgcggta aaaatccaaa ccgcccgtcc
    31081 aatattggcg cgcattccgg taaaacacca gcgaattgga aatttgcaaa tgggtattgt
    31141 cggaacgcgc ccggcgcgta ttcttcaaac gcccccactc cgccgaagac agcgtttgcc
    31201 atctcggggt ttgccaacgg ttgaaataaa ggcgtgcgcc gttggcgtaa gaataagcgt
    31261 cgttgccgta ggtgcggcgt tcgtggaaca ctgccagccc gacatcttta cgccggtcgg
    31321 caaaaccgat gccgccggaa acacctgccg tcatatcgtt gaatttctta ttccccggat
    31381 aagccctgcc ggacacgtcg ccgcccgccg tcgtgtacca gccgtttttc agcgaccatt
    31441 ttttctccgc gccgaaccgg taattgaccg ccgtgccgtc cacctgtttc gggaaagtcc
    31501 aattgccgta ctgctgctgt ttcggggctt ggttgatatt gtgttcgcgg gtaacgctga
    31561 gaccgccgtt taccttccac gcatcgcgtt cgcgcaatgc cttgcggtac agctcgacct
    31621 gctccataag ctgcggcggc agatcttctg ttttcaggcg gtcgaactgg tctgccgccg
    31681 cctcgttctg cctgtcttca aacaatgccg ccgccaaacg catacggacg gcgggcgcgt
    31741 cgggttgggc ggcaatcaat tcccggtaat gggaaaccgc ctccttcacc ctgccctctg
    31801 cctgcgccaa aatcccttgt gcataaagtg ccaacatctt atcctgccgc gcctgttgta
    31861 ggtaaatcgg caaaatcacg cggataccgg caatattgtt tgagaccact gcggaataca
    31921 tggcacgcga caacaattcg ggatttttca gcagggtttc gccgtcaacc tgcaaaactt
    31981 ttcctttttc ccgcacctgc cccggtacct tctccctgtc gatcggtttg acctccgctt
    32041 catgaagcct gaactcggga cggcttctca aatccggttc gcgcggtgtt tcttcggcgt
    32101 atgccgcgct tgccaataaa ggcagcagca tccatctgtt tcgtgcaggc ataaatgtct
    32161 tcccacaaaa ataaaaataa aaaataacca ttatcaaccg gcggattgta tcagtttttt
    32221 atgccgctgt tccaaaccct gccgcccgat gccgcgacaa tgccgtctga agccagaatc
    32281 gggtttcaga cggcggcaaa aaaccggcgg ttttccgccg gtttcatcat tatgccccgc
    32341 ccttctgggc ggcagggttc ggatcggttt gcggggcggc gcacggggcc gtgcaggtgt
    32401 gccgcctgtg tgtcaaaaca tttcccggcg catcaaaaca accatttgac cgacaatgcg
    32461 gcttctttgt cgccgtccgt ccttttgccg tatccgatgc gtgcggacat atggcctttc
    32521 caaccggctt caatgccaaa ccgcccttcg agcgccgtcc tgcctgccag cgtctgtttt
    32581 tcgccgtcca tttccatgcc gaaagatttt gacctgtgca aaacattaaa agcggcaaaa
    32641 ggctgaagat tgacaccgtt acgcaaagca aaacgggttt ttgcccgaat gccggcgcgg
    32701 atttgccact gaccgctgcc gagcagcccg accgccgccc ctcgctgtcg gtaaagccgc
    32761 cgtttacgcc caagtaggta aactgcgcct gcggttgcag gtaaaactgc atattactgc
    32821 cttatatatt gcgtccctaa gaaggggcga ttaacaaaaa ttaacgcccc ttcttaggga
    32881 cgcaatatat aaggagtgag tacctgttct tctattaaag aattaatctt ttgaggcatt
    32941 ttctgttgaa gtttattaaa cacatcttca gaaattccaa actgctttat tatctcttcc
    33001 ctaacccgtt ttttattttc ttcccaagcc tcgggtcttg ttttgtataa atcctgtaat
    33061 tgctttttaa ctgcctgctt taaatcagtt cggtaattat ctgtaatatt gatttcgtca
    33121 tacaaggctt cagatgattt ttgatcggtt ttaccgctgg agaaataatg gaaaacaccg
    33181 tttgccggct tgtctgaaaa tgaaaaatcg gcagtatcgc caacatttgt aggacgagta
    33241 tccgaaccgg gctgataact gaaagcaccg aagcaatcaa aagacacact gcacgggccg
    33301 cgccaaacgt gggaatttct gtcttccgcc gcttctaaaa taaatttgtt tttaatctcg
    33361 gcttctttga cgggatcgta cgcatccgaa ccccaagcat atggcgtgca aaacagcgat
    33421 aaaacagata atgttaatag attaacccga ccgcttgctt gctttcagtg tcatgagaaa
    33481 ttttcctttg tcaagtgtta aagttataat gattatatac tatatcacac tacatcgcaa
    33541 tgaaaaatcg ggaaaaacaa aaaacccctc cgccgtcatt cccgcgaaag cgggaatcta
    33601 gaattttaat gcggcaagaa tttatcgggg atggccgggg tttaaaggtc tgggttcccg
    33661 cctgcgcggg aatgacggtt tagaaatcac ccgaaacgag aaaaagaacc gaaaccggac
    33721 aagtcggatt cccgcgcggg cgggaatgac gataagcgta atttcaatcc gccatattgt
    33781 cttttcactt gcctgaaaac agatttcagc cgcctatccc tgcatccggc ggctgatgta
    33841 gtcggcaatc aaatcccgct tttcccaaaa aaacttctgc attttttgtt cggaatacag
    33901 tttgcggacc agagtgggca gaccgctgtg gacgatggcg gcgatttctt tgttgccttc
    33961 ttcttcctgc attgccgtct gaaaatccaa acccctgcct ttcagatatg ctgcaaggcg
    34021 gttgcggatg tgtgaattga gttcgatgga tttgagcatt ttgcctccgt tttgcggtaa
    34081 ggatttattc gatttgttta tccgcctgat tttatcacgt ttcccaagcg gtttgcctgt
    34141 acaataccgc ttttcggaag ggtgtgggcg atgatgtttt cttggttcaa gctgtttcac
    34201 ttgttttttg tcatttcgtg gtttgcaggg ctgttttacc tgccgaggat tttcgtcaat
    34261 atggcgatga ttgatgcgcc gcgcggcaat cccgagtatg tgcgcctgtc ggggatggcg
    34321 gtgcggttgt accgttttat gtcgcctttg ggtttcggcg cggtcgtgtt cggcgcggcg
    34381 ataccgtttg ccgccggccg gtggggcagc ggctgggttc acgtcaaact gtgtttgggc
    34441 ttgatgctct tggcttatca gttgtattgc ggcgtgctgc tgcgccgttt tcaggattac
    34501 agcaatgctt tttcacaccg ctggtaccgc gtgttcaacg aaatccccgt gctgctgatg
    34561 gttgccgcgc tgtatctggt cgtgttcaaa ccgttttgac ggagcatctt atgccgatag
    34621 aaatcgaacg ccgttttttg attgaaaacg acaaatggcg gcaatacgcc gatgagccgc
    34681 tactgctgaa acagggctac ctgtctgttg aaaaagaacg caccatccgc atccgcattg
    34741 ccggaaaacg ggcgtggctg acgctgaaag gctatatttc ggaaatcagc cgcagcgagt
    34801 tcgaatacga aatcccgctt gccgatgcgg aaaagatgat ggaaacgatg tgcccgttta
    34861 agatggaaaa acggcgttat ccggtcagat ggggcggcag cctgtttgaa gtcgatgttt
    34921 tccttggcga aaactcgcct ttggtcgtcg cggaaatcga gttgcccgcc gaaaatgccg
    34981 attttgaccg tccggactgg ctggggcgcg aaatcacttc agacggtatg tttaccaatg
    35041 cctatttgag caaacatccg ttctcaagct ggaaaaatgc cgtctgaagc ggcttcagac
    35101 ggcatctgcc ttgcggttat cccttgaggt aatgcacgaa tgtatatgca acgcctttgc
    35161 tgctgacacg gcgttccgtc cgctctgctt ctctccaatg cgtccggtct atttctggga
    35221 aaaatgcatc tccttccaca gacaaatcca cttcggttat ccgcaaatcg gtcgctaatg
    35281 gcatcgcttg tccgtatatc tgcgcgccgc ccataatgac ggcttcttcc gcgccggcgc
    35341 acaatgccaa tgccacctcc aaacttgcca ccgtttccgc gcctgccgcg caataatccg
    35401 cctgccggct gatgacgatg ttcctccgtc cgggcagggg tttgacgggc agggattccc
    35461 acgttttccg ccccataatg acgggtttgc ccaaggtata ggctttgaaa aatgcgaaat
    35521 cttcggggat gtgccaaggc atagcattgc ccgccccgat gcacaggttt tccgcacacg
    35581 ccgcaattat ggttattttg agcatatttt ctccacaaaa atgccgtctg aacgcttcag
    35641 acggcatttt atgacattct gcgtcaaaaa tctcaacctg cgcgcgccat acgttttgtg
    35701 ttcgcacccg ccaacaatgc caacaattct tcagtcgcgc cccaaccgat acacgcatcg
    35761 gtaatgctct tgccgtacac ttccggcttg tcctgcctgc cttcgaccaa atggctttcc
    35821 accatcacgc ccatgatatt gccgccgtcc tgttccaatt ggtcggcaat gtcttgtgcc
    35881 acttccatct gccgggtgta atccttgcgg ctgttggcgt ggctgcaatc gatcatcagc
    35941 ttgtcggtta cccctgccgc acgcagttgt tccaccgcct cgctgacgtg tcccgcatca
    36001 taattcggct cttttccgcc gcgcaaaatg acatgacagt cgggattgcc gccggtatgg
    36061 acaatggcgg aatgcccggc cttggttaca gacaagaaat gatgcgaatg gctcgccgca
    36121 ccgattgcgt cgatcgcaat cttcaaattg ccgtccgtac cgtttttaaa gccgacgggg
    36181 caggacagac cgcttgccaa ttcgcggtgg acttggcttt cggtcgtccg cgcgccgatt
    36241 gccccccaag aaatcaggtc ggcataatac tggggtgtaa tcatatccaa aaattcggta
    36301 gaggcaggca tgcccatatt attcagggac aacaacagac tgcgcgcctg acgcaaaccg
    36361 aaattgatgt cgaacgtacc gtccaaatgc gggtcgttaa tcaaaccttt ccaacccacc
    36421 gtcgttctcg gcttctcgaa ataaacgcgc atcacgatca aaagctcgtt ttcataccgc
    36481 ttgcggagtt tcaacagacg ttccgcatat tccaacgccg ctttcggatc gtgaatcgaa
    36541 cacggcccga taatgaccaa cagccgcctg tccctgccgt gaaccaaatc ggaaatttcc
    36601 tgacgggtgc ggtgaaccaa gcccgaagcc tctttggaaa tcggcagctc gtaaagatgg
    36661 gcaatcggcg gcaacaactc tttaacttct ttaatcttaa tatcgtctgt ggggtaatgg
    36721 tgtgtcatct tgcatctttc ctgatgggtt tttatacgct gcttaggtta catcgtttac
    36781 cttgcacaaa caagtgcaac atattactac aaagctattt tatttcaaaa taactgatat
    36841 atcttttttc atagatttta aatttcatat ttttaatatt ttaggaattt tatttcaaag
    36901 aaaaagaaac agattgataa aaacaatacc cgccattcat tcgggcaaaa ccgcacggaa
    36961 catccgtttt cccgtttcca cgacaatccg gtacaggaca ccccgctgcc gattccgcct
    37021 gcacatccgg caaaatttca tacgcaagga aaatgttgaa aacaaaaaga actaattgct
    37081 tgaaaaaaga tttaattgga atgcgggtat ttcccacact ttctccattt atttatcctc
    37141 attataaaaa catgaaaaaa aacccctgct tgcactctca cttgcagccg tgtccgccgt
    37201 tacggtagcc gatattcccg agtctaacgg caaaggcgaa tacaaagtct ttaccgctta
    37261 tgttaaaggc aaagccactg ccggcaattt gaaagaaaaa taacgcacac cgtccatcgg
    37321 ttttcgttca acgaacgcca cgatatgcgt tttaaggcag agtacgcccg aagcaaaaac
    37381 gatgcagacg acaaaagagg gaatgcacac atcgctaacg gcgcaatagg caaaatggct
    37441 gccgtgtatg ccggttatac ctacacccaa ccgggcggcg aatccacacg tttccgtggc
    37501 ggtttggagc gtgtccaagt taaacgttat ctgggagaaa aatgctgcgg ctacgcctgt
    37561 ccaaaaagcg aacgcttcac cctgcaagca gatggtgaaa tccgtcaaaa ccttggcgaa
    37621 ggctggtttc tgaagctatg agcggggtgg ctgtctatct ccgcaacaaa aacagaatag
    37681 caattgcctc ccaagatgcc aacctaaact ccaaaggccg gttcgtcagc agcggtctga
    37741 atgtgggcaa acaactgacc ggcagtctcg gcgtagagtt tgacccatac taccgccacc
    37801 gcgcaatccg caagtctgcc gaatttgtct cgaacactac caaaaccaaa acagacagcg
    37861 aaaagtttaa cgagtagggc ttccgcgtag ccgcaacgtt ctaattccct tacgtataaa
    37921 aagcagcata aaaatgctgc cttttcccat ttccaaggga agtattccgt agtgcagctt
    37981 acctcattgt ttcacacaaa ccgaaaatgc cgtcttaaaa ccaattttca gacggcattt
    38041 gtcccttaaa acacgttttt tcaagcgcga ccataccaaa cacccacagg ttgccttcca
    38101 cccttcgtac aacctttgct ccatcaaaca tccacttcct aaatactgat gtatactg
//
LOCUS       DAXFWM010000014        35731 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000014 DAXFWM010000000
VERSION     DAXFWM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35731
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.014"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(30..593)
                     /gene="orn"
                     /locus_tag="ACFI8B_000667"
     CDS             complement(30..593)
                     /gene="orn"
                     /locus_tag="ACFI8B_000667"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC3833952.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
     gene            complement(611..1498)
                     /gene="prmA"
                     /locus_tag="ACFI8B_000668"
     CDS             complement(611..1498)
                     /gene="prmA"
                     /locus_tag="ACFI8B_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC3833953.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRSGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLNGVKR"
     gene            complement(1602..2963)
                     /gene="accC"
                     /locus_tag="ACFI8B_000669"
     CDS             complement(1602..2963)
                     /gene="accC"
                     /locus_tag="ACFI8B_000669"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC3833954.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            complement(3075..3494)
                     /gene="accB"
                     /locus_tag="ACFI8B_000670"
     CDS             complement(3075..3494)
                     /gene="accB"
                     /locus_tag="ACFI8B_000670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC3833955.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEVGQQVKAGDTLCIIE
                     AMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(3476..3733)
                     /locus_tag="ACFI8B_000671"
     CDS             complement(3476..3733)
                     /locus_tag="ACFI8B_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC3833956.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            3850..4890
                     /gene="queA"
                     /locus_tag="ACFI8B_000672"
     CDS             3850..4890
                     /gene="queA"
                     /locus_tag="ACFI8B_000672"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC3833957.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            complement(5091..8306)
                     /gene="carB"
                     /locus_tag="ACFI8B_000673"
     CDS             complement(5091..8306)
                     /gene="carB"
                     /locus_tag="ACFI8B_000673"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC3833958.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFDPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(8313..8861)
                     /locus_tag="ACFI8B_000674"
     CDS             complement(8313..8861)
                     /locus_tag="ACFI8B_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833959.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(8864..9427)
                     /locus_tag="ACFI8B_000675"
     CDS             complement(8864..9427)
                     /locus_tag="ACFI8B_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC3833960.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(10607..10996)
                     /locus_tag="ACFI8B_000676"
     CDS             complement(10607..10996)
                     /locus_tag="ACFI8B_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC3833961.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(11159..11538)
                     /locus_tag="ACFI8B_000677"
                     /pseudo
     CDS             complement(11159..11538)
                     /locus_tag="ACFI8B_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(11592..12725)
                     /gene="carA"
                     /locus_tag="ACFI8B_000678"
     CDS             complement(11592..12725)
                     /gene="carA"
                     /locus_tag="ACFI8B_000678"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC3833962.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
     gene            complement(13723..16855)
                     /gene="pilC"
                     /locus_tag="ACFI8B_000679"
                     /pseudo
     CDS             complement(13723..16855)
                     /gene="pilC"
                     /locus_tag="ACFI8B_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
     gene            complement(18097..19176)
                     /gene="apbC"
                     /locus_tag="ACFI8B_000680"
     CDS             complement(18097..19176)
                     /gene="apbC"
                     /locus_tag="ACFI8B_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC3833963.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLGIDAEIGTHKVRPGVTTIKGVKNIIAVA
                     SGKGGVGKSTATANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPARLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            19395..19470
                     /locus_tag="ACFI8B_000681"
     tRNA            19395..19470
                     /locus_tag="ACFI8B_000681"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19428..19430,aa:Lys,seq:ttt)
     gene            19543..20052
                     /locus_tag="ACFI8B_000682"
     CDS             19543..20052
                     /locus_tag="ACFI8B_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC3833964.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            20393..20833
                     /gene="nadR"
                     /locus_tag="ACFI8B_000683"
     CDS             20393..20833
                     /gene="nadR"
                     /locus_tag="ACFI8B_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC3833965.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            20948..21448
                     /gene="hpaC"
                     /locus_tag="ACFI8B_000684"
     CDS             20948..21448
                     /gene="hpaC"
                     /locus_tag="ACFI8B_000684"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC3833966.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            21464..22159
                     /gene="murU"
                     /locus_tag="ACFI8B_000685"
     CDS             21464..22159
                     /gene="murU"
                     /locus_tag="ACFI8B_000685"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC3833967.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(22193..22624)
                     /locus_tag="ACFI8B_000686"
                     /pseudo
     CDS             complement(22193..22624)
                     /locus_tag="ACFI8B_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            22799..24475
                     /locus_tag="ACFI8B_000687"
     CDS             22799..24475
                     /locus_tag="ACFI8B_000687"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC3833968.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            24589..25680
                     /gene="ychF"
                     /locus_tag="ACFI8B_000688"
     CDS             24589..25680
                     /gene="ychF"
                     /locus_tag="ACFI8B_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC3833969.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            25718..25957
                     /locus_tag="ACFI8B_000689"
     CDS             25718..25957
                     /locus_tag="ACFI8B_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833970.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            25954..27828
                     /locus_tag="ACFI8B_000690"
     CDS             25954..27828
                     /locus_tag="ACFI8B_000690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC3833971.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLVGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYALPMLVLGAGSFFAMRLPFMAQYDRLGLTRSNTSCHN
                     NTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYA
                     SRVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRML
                     HKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGCRQTLRPDDESTLKANARIRELA
                     AKYPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH
                     "
     gene            27890..29185
                     /gene="tyrS"
                     /locus_tag="ACFI8B_000691"
     CDS             27890..29185
                     /gene="tyrS"
                     /locus_tag="ACFI8B_000691"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC3833972.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
     gene            complement(29987..30801)
                     /locus_tag="ACFI8B_000692"
                     /pseudo
     CDS             complement(29987..30801)
                     /locus_tag="ACFI8B_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
     gene            31209..32129
                     /gene="ribF"
                     /locus_tag="ACFI8B_000693"
     CDS             31209..32129
                     /gene="ribF"
                     /locus_tag="ACFI8B_000693"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC3833973.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            32271..35060
                     /gene="ileS"
                     /locus_tag="ACFI8B_000694"
     CDS             32271..35060
                     /gene="ileS"
                     /locus_tag="ACFI8B_000694"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC3833974.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKAHSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(35477..>35731)
                     /locus_tag="ACFI8B_000695"
     CDS             complement(35477..>35731)
                     /locus_tag="ACFI8B_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3833975.1"
                     /translation="IDILTVPHGKNIKSTTYNISPQSQNAHHQSDSIRRVGLGVIAGV
                     GFDITPKLTLDAGYRYHNWGRLENTRFKTHEASLGVRYRF"
BASE COUNT         7711 a         9478 c         9928 g         8614 t
ORIGIN      
        1 atagcgtttc agacggcatt gtttgtttct tattgcgctt cggctttcgg ggcggaaatc
       61 aggaagtgtt cgcggtagtg gcgcatttct tcgatgctct ccaaaatgtc gtccaatgcc
      121 ttgtgcgaac cgcgtttgac gacgcttttg gcaatgggcg gattccagcg tttggcgagt
      181 tctttcagcg tggaaacgtc gaggttgcgg tagtggaagt agttttccag tttcggcata
      241 tatttgacca taaaacgccg gtcttggtgg atggagttgc cgcacatcgg tgtggcgcgt
      301 ccgggtatcc attccgacat aaagtccagc agtttctgtt cgacttcggc ttcggtatgc
      361 gacgattcgc gcacgcgctg cgtcagcccc gtcctgccgt gtgtggcggt gttccactcg
      421 tccatattgt cgagcagatc gtcgctttgg tggatggcgt aaacttcgga ttgcgccaac
      481 acattcaaat ccgagtcggt aataatcatc gcgacttcga taatgcggtc ggtttcggga
      541 ttcagccccg tcatttccat atcgagccag cagaggttgt ttttgtcctg cacggtgttt
      601 cctttccgtt tcagcgtttt acgccgttca agcgcgccca gccttcgtca gtttccgccg
      661 ggtcgagatc gaaccattgg ctgtaaatgc cgccgagttc ttcggcctgt tcatccaaca
      721 aaccggacaa cacgatgcgt ccgccctgtt tggtgcgggc tgcaagcatt tcgccgagca
      781 tacgcaaagg gttggcgagg atgttggcga caactacgtc gaattgccct tgaggcagac
      841 tgtcgggcag gaagaattgt gcatcgacgt tgttctgttc ggcgttgtcc ctgccggagc
      901 ggacggcctg ttcgtcaata tccacgccga cggcggaacc tgcaccgagt ttgagggcgg
      961 cgatggtcag gatgcccgaa ccgcagccgt agtcgaggac gctttcgccg tttttgagtt
     1021 gcgtatccag ccatttgagg cagaggcgcg tggtcgggtg gctgccggtg ccgaaggcga
     1081 gtccgggatc gaggcggagg ttgacggcgc agccttcggg ggcttcgtgc caagaggggg
     1141 taatccacag gcggtcggaa attcggatgg ggtcgaattg cgattgcgtg aggcgcaccc
     1201 agtcttggtc ttcgatggtt tcgccggtgt atgccaagtc ttttaacccg cattcttgtg
     1261 cggcggcatc gatgacggcg gcggcttcgt cgtgttcgcc gaacagggcg atgactttgc
     1321 tctgctgcca gatttgttcg gtgggcatac cgggttcgcc gaaaatcgcc tgttcgtttt
     1381 gcgtgccggc gcaggcatct tcgatggcgg cggagagtgc accgtgttcc atcagcgcgt
     1441 cggcgaggcg ttcggcgacg gcatcgttga cgttgacggt gatttgttgg tagggcataa
     1501 cgggctttct tggaaatatc gaaatcggtt tcagacggcg gggcaaggga aatgccgtct
     1561 gaacgcggaa gggcggcttc agacggcata tcggcggcgg cttatttgtc ctgtttggtt
     1621 ttgcgcgctt ccagccagtg ctccaggtaa tggatgctca ctccgccttc ttggaaaccc
     1681 gcatcggcga acaggtcgcg gtgcaacggc gtattggttt tgatgccggt taccgccagc
     1741 tcggcgagtg cgacgcgcat tttcgccatt gcctgttcac gcgtttttcc gtgtacgcag
     1801 attttgccga tcaggctgtc gtagtacggc gggatgcggt agccttggta gatgtggctg
     1861 tccacgcgga tgccgaagcc gccgggcagg tggcagcttt caatcgggcc cgggctgggg
     1921 atgaagttgt acgggtcttc ggcattgatg cggcactcga aggcgtggcc ttcgattttg
     1981 atgtctttct gtttgtattg cagcggcagg ccggatgcga tgcggagttg ctcttggacg
     2041 atgtccacgc cggtgatgag ttcggtaacc ggatgctcga cctgaacgcg cgtgttcatc
     2101 tcgataaaga aaaattcgcc gtcttcgtat aaaaattcaa acgtgcccgc gccccggtag
     2161 ccgatgcgtt tgcacgcgtc ggtacaggct ttgccgattt ttttgcgtgc ctcttcatcg
     2221 ataaacgggg cgggtgcttc ctcgatgact ttctggtggc ggcgttgcag cgaacagtcg
     2281 cgctcggcaa gatagacggc gttgccgtgt tcgtcggcaa gcacttggat ttcgacgtgg
     2341 cgcgggcgtt gcaggtagcg ttccatataa accatcgggt tgccgaacgc cgcgcccgct
     2401 tcagccttgg tcatttcgac agactggagg aggtcttctt ttttctcgac cacgcgcata
     2461 ccgcgcccgc cgccgccgcc cgatgctttg ataatcacgg gataaccgac tttgtcggcg
     2521 attttgagga tttcggcatc atcgtcgggc aatgcgccgt cagagccggg gacgcagggc
     2581 acgcctgccg ctatcatcgc gtgtttggcg gagactttgt cgcccatcag gcggatggtg
     2641 tcgggtttcg ggccgatgaa ggtaaagccg gactgttcga cctgttcggc gaaatcggcg
     2701 ttttcggcaa ggaagccgta gcccgggtgg acagcgtccg cgcaggttac ttcggcggcg
     2761 gcaatgatgg cgggaatgtt cagataactt tgcgcagaag cggcaggacc gatgcacacg
     2821 gattcgtcgg cgagtttgac gtgcaggctg cctttgtcgg cttcggaatg cacggcaacg
     2881 gtggcaatgc ccatttcgcg gcaggcacgg agtacgcgca aggcgatttc gccccggttg
     2941 gcgattaaaa cttttttcag catgatgacc tttcctgcgg ttccggcaac ccgtccgtaa
     3001 aaaagggtgc gggaacgctg aagggggaaa gcatttcaga cggcatcgga agtttatgcc
     3061 gtctgaaaac aggattatcc gatgatgaag agcggttcgc cgaattcgac gggcgtaccg
     3121 ttttcgacca gaatttcttt gaccgtgccg gatttttcgg cttcgatttc gttcatcagc
     3181 ttcatcgctt cgatgatgca cagcgtgtcg ccggctttga cctgttgacc gacttcgaca
     3241 aatgcggcgg cattcgggcc gggcgcgcgg tagaacgtgc cgaccatagg cgatttttga
     3301 gcgtcggaca aatcgcgggc ggccggcgtt acggccggcg cggcggcagg tacgggcgcg
     3361 gcgtaaacgg gagctgccgc agctgcaatg gtacgggtaa tgcggacttt ttcttcgcct
     3421 tcggttactt cgatttcggc gatacccgat tcttcaacca aatcaatcag tttttttaat
     3481 ttgcgcaaat ccatttctgt tcctttaaag gctgccggtg ttccggcggg ttgttgcgtt
     3541 ggtttttcag aaaacagccg gcttgcggca acggcgcaaa ccgggcggaa tggtgttcgg
     3601 atacggtttt cctgtggtgg gaaacgaccg tgaaattgtg ttcattttcc cgaagttgtc
     3661 ggtaaatgtc cagtaaaata tcaaaaaacg gcggtttttg ggggaaatgt gcaggaagtt
     3721 tgattttgcg cacaagatgc cccgagtcaa gagtgtttat tctaatctgt tggtttttcg
     3781 ggcaaagatg ccgtctgaaa aagggctaaa gtgcgtataa tggcggcttg cccgaacgag
     3841 agtgtaaaaa tggatatttc agattttgac tttaccctgc ccgaacacct gattgcccag
     3901 catccgcccg aggtgcgcgg cagcagccgg cttttggtcg cgctgcccga tatgccgctg
     3961 caagaccggg tgtttggcga tttgcccgat tatgtcgagg caggcgacgt tttggtattc
     4021 aacaacacca aagtcatgaa ggcgcggctg tttgggcaga aagacagcgg cggcaggatc
     4081 gaagccctga ttgagcgtgt gttggacaac cataccgcat tggcgcacat ccgttcgtcc
     4141 aagtccccca agcccggtat ggggctggtg tttgaaggcg gtatccgtgc cgtgatggtc
     4201 gggcgtgagg gcgaactgtt ctgcctgcgt tttgaaggcg gtcaaaccgt ttacgaactt
     4261 ttggaacaga acggacacct gcccctgccg ccttatatcg aacgtgccgc cgatgcggac
     4321 gacgacagcc gttatcaaac tgtttatgcc aaatatcagg gcgcggtcgc cgcgccgacg
     4381 gcgggcctgc attttacgga agaacttttg cgccgtctga aagacaaagg cgcggtaacc
     4441 gcagaagtaa ccctgcacgt cggtgcgggg acattccaac ccgtgcgcgt cgataaaatc
     4501 gaagaacaca aaatgcacag cgaatggttt gaagtgccgt ctgaaaccgt cgccgccgtt
     4561 gaggcggcaa aagcccgggg gaacaaagcc tgggcggtcg gcacgacttc catgcgcgcc
     4621 ctcgagtctg ccgcgcgcgc aacgggatat ttgaaagacg gacagggcga caccgatatt
     4681 ttcatcacgc cgggctaccg ttttaatgtt gtcgacaggc tggttaccaa ttttcatctg
     4741 ccgaaatcga cgctgctgat gttggtcggc gcgttttcgg gtatgggtca tatccgcgcc
     4801 gtgtaccgtc atgcgattga acgtgaatac cgtttcttca gctacggaga tgcgatggtt
     4861 ttggggcgga acgaaggggg cgggctttaa actgctgctg tccgttgcaa ggcagatgcc
     4921 gtctgaaccg tggttcgggc ggtattttta tggatgtccg gcagttggat aatccaccgc
     4981 cccaaattag ggtgcttaaa ggtcaaaaga aagtgaaggc tatgtgcaac aaaatgccgt
     5041 ctgaaaccgc aaaacggctt cagacggcat tgttcaacct gattcaggca tcagttgcgg
     5101 tttttcaaac gaccgtgcag ctcttgaacg ctgtacacac ccagataatc ccggcttttc
     5161 gcgccttcgc tcatcgcttc gccgccggcg gtggtggtgt attgcggcac gcgttgctgc
     5221 aatgcgcttt ggcggatgat gtggctgtcg gacacggatt gcggatcgct ggaaacggta
     5281 tttacgacca gtgcgatttc gccgtttttc agcgcgtcgc cgatgtgcgg gcggccttcg
     5341 gggactttgt tgatggtctg cacaatcagc ccgtgttcgg tcaggtattg cgccgtgccg
     5401 cgcgtggcgc agatgccgta gcctaaggct tggaagtttt tggcggtttt aatgacgcgt
     5461 tctttgtctt cttcgcgcac ggagaggaag attttgccgg tcgggttgag gcgttcgccc
     5521 gcgccgagtt gggctttgta gtaggcttcg ccgaaacttg cgcccacgcc catcacttcg
     5581 ccggtggagc gcatttccgg actcaaaatc gtatccacgc ccgggaattt gatgaatggg
     5641 aacacggctt ctttaacggc atagaaatcg gggacgactt ctttttccac gccttgttct
     5701 ttcagggaaa tgcctgccat gcagcgcgcg ccgactttgg cgagcggcac gccggtggct
     5761 ttggagacaa aggggacggt acggctggcg cgcgggttca cttccaacac gaacaccacg
     5821 ccgtcctgca cggcaaactg cacgttcatc agtccgacca cgcccagcgc gtacgccatc
     5881 gctttggttt ggcggcggat ttcgtcttgg atttcttcgc ttaaggagta tggcggcagc
     5941 gagcagccgg agtcgccgga gtggatgccc gcctgttcga cgtgctgcat gatgccgccg
     6001 ataaccacat ctttgccgtc tgaaacgcag tccacatcga cttcaatcgc gttgttgagg
     6061 aagaaatcga gcagcacggg gctgtcttcg gaaacctgca cggcttcgcg catgtatttt
     6121 tgcaaggctt cggcggagtg gacaatctgc atcgcgcgtc cgcccaaaac ataagacggg
     6181 cgcacgacca gcggatagcc gatttcttcg gctttgacga gtgcttcttc ttcgttgtgg
     6241 gcgatgcggt tgggcggttg gcgcaggcct aagtcgttca acactttttg gaagcgttcg
     6301 cggtcttcgg cggcatcgat gctgtcggcg gatgtgccga tgatgttcac gccgttttca
     6361 accaatgcgt tggcgagttt cagcggggtt tgaccgccgt aatgaacaat cacgccccac
     6421 gggttttcgg tgcggacgat ttccaacacg tcttccaatg tcagcggatc gaaatagagg
     6481 cggtcgctgg tgtcgaagtc ggtggacacg gtttcggggt tgcagttgac cataatcgtt
     6541 tcaaagcccg attcgcgcag ggcgagtgcg gcgtgaacgc agcagtagtc aaactcgatg
     6601 ccctgaccga tgcggttcgg gccgccgccg agaatcatca cttttttacg gtcggaagga
     6661 cgggattcgc attcttcttc gtaagtggag taaagatagg cggtttcggt ggcgaactcg
     6721 gcggcgcagg tatcgacgcg tttgtaaacc ggatgcagct tcagcgcgta gcggtgttcg
     6781 cgaacttctt tttcgcttac gttcaacaat tgtgccaaac gtttgtcgga gaagcctttg
     6841 cgtttcagac ggcgtagggc ggcgtaatcc aaatcttgca actggccgtc tgaaaccgat
     6901 ttttcttcct tcatcaagtc ttcgatttgc gccaagaacc aagggtcgat ggcgcagatc
     6961 tcgtggattt cttccggcgt gaagcccgcg cggaacgcgt ctgccacaaa cagcatacgt
     7021 tcggggccgg ggttggccag ttcgcggcgg atttccgctt tgtcttcgct gcgcggattg
     7081 aaaccgcaca agccggtttc caaaccgcgc aaggcttttt ggaagctttc ctgaatggta
     7141 cggcccatcg ccattacttc gcccacagat ttcatctgcg tggtcaggcg gtcgtctgcg
     7201 gcggggaatt tttcaaacgc gaaacgcggg attttggtta ccacatagtc gatggaaggc
     7261 tcgaacgacg cgggcgtgcg gccgccggtg atgtcgttgc gcaactcgtc cagcgtaaag
     7321 ccgaccgcca gcttcgccgc caccttcgca atcgggaagc ccgttgcttt ggaagccagc
     7381 gcggacgaac ggctcacgcg cgggttcatc tcgatcacaa tcatctcgcc gttttcaggg
     7441 ttcaccgcaa actgcacgtt cgagccgccc gtgtccacgc cgatttcgcg caataccgcc
     7501 aacgaagcgt tgcgcatgat ttggtattcc ttgtccgtca gcgtttgcgc cggcgcaacc
     7561 gtaatcgagt cgcccgtatg aacgcccatc gggtcgaagt tttcaatcga acagatgatg
     7621 atgcagttgt ccgccttatc gcgcaccact tccatctcgt actctttcca gccgagcaca
     7681 gactgctcaa tcagcagctc atgcgtaggc gacgcatcga aaccgcgttc gcaaatcgcc
     7741 aaaaactcat ccttattgta ggcaatgccg ccgcccgaac cgcccatcgt gaaagacgga
     7801 cgaatcagcg tcggaaagcc gacctgttct tgcgccgcca aggcttcgtt catggtgtgg
     7861 cagacaaaag atttcgggca agagaggccg attttttcca tcgcttcttt aaagcggccg
     7921 cggtcttccg ccttgtcgat cgcgtcttcc gttgcgccga ttaactcgac attgtatttc
     7981 gccagcacgc cgttacgcgc caaatccagc gcacagttca gcgcggtctg accgcccatc
     8041 gtgggcagaa tcgcatcggg ccgctccttg gcgataatct tctccaccgt ctgccacata
     8101 atcggttcga tgtaggtaac atccgccatt tcagggtcgg tcatgatcgt ggcggggttg
     8161 gaattcacca gaatgacttt atagccttct tcacgcaaag ccttgcaggc ctgtgcgccc
     8221 gaatagtcaa attcgcaggc ctgaccgata acgatagggc cggcgccgat gataaggatg
     8281 gattttaggt cggtacgttt gggcatgggt ggttactctt gaattaaaga aggttgtttt
     8341 gttacatagc ttttaagaaa agtatttccc ttttctaaaa ttgaagctaa aacatcatct
     8401 tctatgttag ataaaatatt ctctaaatct gcgcttgatt taattttaaa gaattctttt
     8461 gtttgagaat ggtatacaag agaaggttta tttagctgta aaagttctgc tatattattt
     8521 aatgaaccaa tactcttacc atcccataaa ataaagccat aatcagcaat ttctgccatt
     8581 ttcttatctt ttgctgtata aaataccctt ccagttcctt tgctgtctac ttggacaaac
     8641 tgccaatttc ccacattatt tcggtaaatt ttgccagaaa agtaaatatg cacattagca
     8701 taatcttgtt cttgtagaaa ttcttggata gctttatcag ccccattagc atctccaata
     8761 acaatatcaa aattattgct aagaatattg ttaattcttt ctcgtatttg tggatttagt
     8821 cgtgaaatgc tgcgtgaacc agaaaagaaa atagtgcgca tttttatcct ctagtctttg
     8881 tcattgctaa aacaattaca gatctagcat tttttgaaaa caatttttca gttgatattt
     8941 ccaatgttgc tccgctatca aataaatcat cgattactaa aatattctta ttggatagat
     9001 ctatattgtc aattgtgatg gaattattca gtatttctag tttttctgat ttttcttcaa
     9061 tatttttgag tggtgtgtgt gagctagatt ttcttaaaat aggagaatat ggaatattca
     9121 agcgatcact tagttctttg gcaattaatt gaactggttg attaatacgc tctgttgtaa
     9181 aaggggcggg aacaattaaa tttatagatt ctaaaccact aaatttttgt aaaatatagt
     9241 ctaccagcaa agaaacattt tgggtttgat tacgatattt taattgataa acccattctc
     9301 caatcacact tctcttgcta tcaaagtgag gatgccctaa ttcatcataa cctaacaaaa
     9361 tacttttttg catatgatgg tctaaagcaa atccttttgt ccagttacca tttagttcta
     9421 tcgccatttc attgactcct tgtcaaaatt aagtataacg agcaatccta gcaagccgta
     9481 gcccgcatgt agggtgtgcg gtacgcacgc acgcgttctt cattttccct gtaaccccga
     9541 cccaccgcat gcgcgccttg cggcacacac tctaccgatg agttcaaaga ttgtctgaaa
     9601 aaacgctcgc ggcgtttgtt ccatctgctt gacaataaag gctacctgaa aaattcccgc
     9661 tgttgatatt tcgtgcatcc tttttcagac gacctgaaat caacggttgc aaatttaagt
     9721 tatttttcaa accaccggca accgaaaaat ctgccgaaac ccactccctc ccccgtgggg
     9781 gagggctggg gggagggcat tctccgaatg gtggcaatct ttcctaatac cttcgccgcc
     9841 caaatacaag ccttgcggct tgttgccctc tcgctagctc tctcccacag ggagagagga
     9901 cgggtcggct gttggggttg aggtttgctg caaggaaaac aggttgtgca ggctgctttt
     9961 tatttttcag acgacctgaa acaacagctg caaatctaag ttgcctttca aatcgcctgc
    10021 aaccgaaaaa tctgccgaaa cccactccct cccccgtggg ggagggccgg ggaggcggca
    10081 ttctccaagt tgcggcaacc tttcccaaca gcttaaccgc cccaatacaa gccttgcggc
    10141 ttgttgccct ctctccagcc ctctcccacg gggagagagg acggggaggc tgttggaatt
    10201 aagggttttg taaactaatc aggttgtgca ggctgctttt tgcttttcag acgacctgaa
    10261 acaacagctg caaatctaag ttgcctttca aatcgcctgc aaccgaaaaa tctgccgaaa
    10321 cccactccct cccccgtggg ggagggctgg ggagagggca ttctccgaat ggtggcaatc
    10381 tttcctaata ccttcgccgc ccaaatacaa gccttgcggc ttgttgccct ctctccagct
    10441 ctctcccaca gggagagagg acgggtcggc tgttggggtt gaggtttgct gcaaggaaaa
    10501 caggttgtgc aggttgcttt tttgcttttc agacgacctt tttaaacgct caattcagtt
    10561 aattttacct tattattttc aaaatctaat caaaatcaac cgtttgctac cgtgctggct
    10621 gcttttccaa ttcctgcaat acgcgcagga tttccgccag tacatcgttt gtctgctgca
    10681 aaatttcgtg attccaaaaa cgcagcacgg taaagcccaa gctgttgaga tataccgtcc
    10741 gcgcgtggtc gtatacggct tgttccgcgt gctgcccgcc gtctgcttcg acaatcagct
    10801 tgggcgttac gcacataaaa tcaacaatat aattccccat cggctgctgt cggcggaatt
    10861 tatagccgtt cagacggcct gcccgcaggt gttgccacaa ttttgcttcc gcctcgctca
    10921 tttcttgacg catggctttg gcgcgttggc gtagggcggg gttttcggcg gtcaatagtt
    10981 tctcgggcgg gttcattttt gttatcgggt tgctgtttgt gtaagcctta cagcttgttg
    11041 ccctctctct aactctttcc cacagggaga gaggacgggt cggctgttgg ggttaaaggg
    11101 gctgcaagga aaacagcttg tgcaggctgt tttttatttt gcataatccg tctgaaaatt
    11161 aataactgct gatttccagc aggttgccat cgggatcgcg caggtaaacc gattggattt
    11221 tgcccattgc gcctgtgcgc gctacgatgc cgcttaaagg tttgatgccg tgtgcggata
    11281 attcctgtaa aaccgtttcc agtggggcgt atcggtcagc aggcataaat ccgctgtgcc
    11341 gcaggcggcg tgttgcgcgt taggctgaat ttccgcaccg cgcccgtgta ggttgatttt
    11401 ctgactgcca aacaacaaag ctttacggtt gttgccaaat gaaacttctt ccatgcccaa
    11461 aacttgtgtg taaaacgcga tggttcggtc aatgtcggca acagtcagta ctagatggtc
    11521 gagtgcgcta attttcattt ccagctttct ttttcagacg gcagcagcat actgttgccg
    11581 tctgaaaatc attatgcttg ttttgcagcc ttcatattgc caatgaattt gtcgaacaaa
    11641 tagccgacat cttgcggacc cgggctggct tcggggtgtc cttggaaaca gaacacgggt
    11701 ttgtcggtca gctcgatgcc ttgcaaggta ttgtcgaaca aggatttgtg ggtaatgcgt
    11761 gcgttggcgg gcagggtgtc ggcatcgacg gcgaaaccgt ggttttggct ggtaatgacg
    11821 actttgccgc tgtccaaatc ttgcacagga tggttcgcac cgtggtggct gaagcgcatt
    11881 ttcagggttt tcgcgccgat ggcgaggctg atgagctggt gtcccaagca gatgccgaaa
    11941 atcggtttgc cgctttccat cagtttttgc acggcttcga tggcgtaggt gcaaggctcg
    12001 gggtcgccgg ggccgttgga caggaacacg ccgtcgggat tgagtgccaa cacgtcttcc
    12061 gcgctcgttt gtgcggggac gacggtcagg cggcagccgc gcgaggcgag catacgcagg
    12121 atgttggttt tcacgccgaa atcgtaggcg acgacgtggt aaggctgttt gtcaggggta
    12181 acgaaacctt tgcccaattc ccattcgcct tccgtccatt cgtaagtttc cgtgcaggaa
    12241 acttcttttg ccaaatcttt gccgaccatg ctgccgaatg cggcgatgag ttcttgcgct
    12301 ttttcaacgg tggcatccgc acctgtcaga atcgcgccgc cttgcgcgcc tttttcgcgc
    12361 aacagcatgg tcaggcggcg ggtgtcgatg tcggcgatgg cgacggtttc gttgcgtacc
    12421 aaatagtcgt gcaggctttc ggaggcgcgg aagctgctgt gcaagagcgg caggtcgcgg
    12481 ataatcaggc cggcggcata aacgctgcgg ctttcttcat cttcggcgtt ggtgccggtg
    12541 ttgccgatgt gggggtaggt gagggtaacg atttgtttgc agtaggacgg atcggtcagg
    12601 atttcctgat agccggtcat cgaagtattg aacacgactt cgccggaagc cgaaccttcg
    12661 taaccgattg atgtgccgtg gaatacgctg ccgtcagcga ggacgaggag ggcgggggtg
    12721 ctcatgatgg gaatcctgtt tttaataaaa ttcttgacaa tgccgtctga agggacttcg
    12781 gcagggggca tccggtcgga aaaatccggt caaaaaaaaa cacgccgcag aacaaaaccg
    12841 cgtaacgtgc ttttcgggca agtgcctata ccctgaaagc agcatatttt aagacgaaac
    12901 atacccgatt tcaagcgcaa acgcggcagg cgggctgata tgttcgcgcg aaaacggaaa
    12961 tccggggcat aaaaccgccc gctccgcgcc ccctatgccc gttacgcatt tccccacgtc
    13021 cgcccggcga aactatggga atacccgaac cgtcattccc gcgcaggcgg gaatccggac
    13081 ctgtcggcac ggaaacttat cgggtacaac ggtttcttga gattttacgt cctagattcc
    13141 cgcctgcgcg ggaatgacgg catcggtttg acggtattta attgaattgt ggaaattgac
    13201 ggattcagtg agattggcgg gatgaagcct accctgtagc ccgcctttta tagcggatta
    13261 acaaaaaccg gtacgggttg ccccgccccg gctcaaaggg aacggttccc taaggcgccc
    13321 aagcaccggg cgaaccggtt ccgtaccatt cgtactgcct gcggcccgcc gccttgtcct
    13381 gatttttgtt aatccgctat acggacccgc cccctcccga aaaacgcaaa aaatgccgtc
    13441 cgaaaacttt tcggacggca ttttcgcgtg aaccgtcatt cccacgaaaa cagaaaacca
    13501 aaatcagaaa cctaaaattc gtcattccca cgaaagtggg aatccaggac tcaggacctg
    13561 agaaaccttt ttacccgata agttcccgta ccgaccggtc cggattcccg cctgcgcggg
    13621 aatgacgcgt ttcatttgcc gccccctccc gaaaaacgca aaaaaatgcc gtccgaaggc
    13681 ctttcggacg gcatttgcgg aaaaaccggc cgcgcaggcg ggtcagaaga agacttcgcg
    13741 ccagcttaag cgtttgatac cgcacatcgg gccggtaata tccaagctgt ccaaatcgtt
    13801 catcagcagg gtgcgcacac cttttccgga gaagcaccgg ttattgcggg cgggtttttt
    13861 accctctttg aatgttccgc tgccgcctgc atcaccgtct gccgtgacgg ggaaatcgtc
    13921 tgttttcttt tcatccagat aacgcacatt aaccggtttg tcgtaaacat atccgttcgg
    13981 gcagacggtt ttaccgcctt tttccataca acctataggg atggatttgc ctttggaggt
    14041 tgtcttatgg ccggaatatt gtgcgacagc cgtattggct tccggcacaa tcgggcgcgc
    14101 gcttctcgga gtcaatgcgc cgccgtcggc ggtattgatg cccaaaatgg cggtttccgc
    14161 gccgcagccg ccgtcgttat atttgcggat ggttacgaag gcggtacgca ataccacggt
    14221 cggtttgacg gtaacgcgtt ctccttccct caatttcact acccaccctt tatcggccga
    14281 tccgtcggat ctcttgttca ggaataatgt tttattttcc tcgctaagca cttgctcgag
    14341 cagcccgcct cccgtgccgt ttgttacctt tacattaacc gtcggcttat cgtcgtcaaa
    14401 gataccgtaa atatattgtt ctttcgtatc gactacatcc ccttcactca aatcgctgcc
    14461 cgtgccgaag ataaccacgc gtttgtctgc cagtcgggaa acggcgggcg cggaggtaat
    14521 cggttttgtg ccttcgaaaa tagcctttgc agaccattta ttaggatcgg aattgctcaa
    14581 atcaaagcgg tacatattgc cgccccggtc gccggcatag gcgatatcga ctgtgccgtc
    14641 caaatcttta tccaccagcg tgggggacga aagcccgccc ttgccgccgg atacttggat
    14701 tgttttaatc agtttaccac cggtgttttc caaatcatac acatacagcg cggttttatt
    14761 ttcttggctg gcaatatctt tagccgcata accggaggcg aggaaggcgg cgtatttgcc
    14821 gctgcgggtt ttgccgattt gcggcgtgcc gacggtgtag cctaatttca cgcgattatt
    14881 gttatttttg tcgttttgga catcaaacat ggaaacgccg accgggttgc tgtcgatttt
    14941 ggtcaaatcc aaggcgtatg cgcctctgcc gccaaggccc attgcgccga acataaagaa
    15001 atgtttttgc ctgtcctggt catctgtaat gcggcgcaag acaaagccgc cgtccacgcc
    15061 gtagcggtcg cccacatagc ctttttcggc aaaggtgcgc agctcttggg cgagggtgga
    15121 gtctttgaga gcggaagtgt cgttatcaaa atattggcgc ggcatcgtgc cggggatgta
    15181 gctgagcttc agattgtagc tgcgttcatc actgccgccg ttttttttga agatatgcac
    15241 catcccgtcg ttggcagaag ttgccaaata cccgtcgacc gcgacaatcg ggctgttgac
    15301 gatgtcgccc aaatcgcggg gtttgtcatt gtcatcttct tttgtgcgga tgcggtattt
    15361 ttggctgtat tgttttttgt cgctttgttt tttgttgttt ggttggttga atgttttaaa
    15421 tatatcgtca ttaccgtaat accgggccgt ccaaggcagc agcacttcgc tccactcgtc
    15481 ggccacaggt gtgacgagcc tgtccttgaa gatgccgaaa gtgtcgtttt tgccgtcatt
    15541 gccattaata cccgcgaccc ggccgccgtt cttgcccgag ttgatcttgt acacgccgcc
    15601 cggcaatcgg atgacggtct gccttgagtt gaaattcggc tcgcgggatt tgatatcctg
    15661 cgtattcaaa gcggcgaggg aatgccggcc gggcctgccg gggtcggttt tagttttcag
    15721 ctcttggagg aagatgcggc tgctcgagct gccggggtag gtagaaaccg aagcggaata
    15781 catctgcacc ttgccatctt gcccgggtcc gaaccacagc gcgggggcgg tcagtgccgg
    15841 cgaaggggct ttggtattat tattggggtt cggtttgttg atgcagcggc ctgctttgac
    15901 ttgcggcagg ccgagcttga ccggaatctg cccggtatct ttaatgtccc acgtagccca
    15961 aaaagataga atgccgctaa gccccgtatt tcctttccgc acatcgatgc tcggcttcaa
    16021 gacacccaac cgcactgttt tgctggtgat atctttaatc ccttttttgt ccagcaggtg
    16081 cagtttggcg ttcagataat aggccatggc ggtactttcg cgctgccagt cgcccaagga
    16141 aatattgtgg ccttccgtgg tatagacgag gttttgatta ctattgtttt catcaaaaga
    16201 agataccaat ttgttggtta ccttaccatc tttggcggta ccctcggcgc ccaaattgaa
    16261 agacacgccc aaccatggat aatccttcaa tttgtaaatg ggcgaattgt cgcggcttgg
    16321 ctctgtgtgc ctattcagcc tgcctcctgt ttttgccaag tcgggattat cgaaggaaaa
    16381 tcgggtttta tagacaagtt tgggacagcc tgtggtgctg ttgcatacga caccggcgta
    16441 gctgtagcct gccagcgcga tgcgggtggt acgaatccaa tcaacggcat tggcgcgccc
    16501 tttcagacta tcggtatcaa aaccggaaac cttgccgtag ggcggcaggt aggtggctgt
    16561 gccaaaaacg gcagtaccgc tttgttgaga aacaagctca tcgttattgt tgaatgagac
    16621 agagcttcct cctcgtgggt acttatgatg agtatattcg cgcttcctgt ctttttccct
    16681 taaggttgaa tatgaaccct cccactttac ctccggctga tttcgttcgt tcatgataat
    16741 agcgtattca cgggtttgcg cctgcgcccc cccccccgcc ggtatgggaa aacatcaaga
    16801 tggcggcata aagcgcggta tggcggaaaa cccgcctttt taaagtttta ttcatcgtat
    16861 ttccttttcg gtcaaacccc cccgcccttt ggggcggcag gatcaggctt tgtccgggag
    16921 gggtgcaagc ccttccgaat cagggcggcg ccttatgtgc cgccctgcgt gttggaacat
    16981 attttatccc ttaaatcttg cttcgcggga taccgtcggc ggcaggcgcg ccgcaaacgg
    17041 cgggattggg caaaccgccc gatcccgcgc ccgccggatt gccggttgca aagccccctt
    17101 cgcccggctg ccaaaggggg atgttcgcaa aggtaacgcg cccctctgac ggggtgcaat
    17161 gtatagggtt gccgcatcat tctatccttg cgcgcttatc catggtacag cggtttttgg
    17221 caatccgcaa ccgtttgccc ggcccgccat tgccgcgaaa gtgggaatcc ggaacgtaaa
    17281 atctcaaaaa accgttttat ccggcaagtt tgcgcaccgg caggcccgcc attgccgcct
    17341 gcgcggcaat ggcgggccgg ggaggcggca ttctccaagt tgcggcaacc tttcccaaca
    17401 gcttaaccgc cccaatacaa gccttgcggc ttgttgccct ctctccagcc ctctcccacg
    17461 gggagagagg acggggaggc tgttggaatt aagggttttg taaactaatc aggttgtgca
    17521 ggctgctttt tgcttttcag acgacctgca tcaacggttg taaatccaat ctgtttttca
    17581 aaccgcctgc aaccgcaaaa cctgccgcaa tccgctccct cccctgtggg ggagggccgg
    17641 ggagagggca ttctccaagt tgcggcaacc tttcccaaca actcaaccgc cccaatacaa
    17701 gccttgcggc ttgttgccct ctctccagcc ctctcccacg gggagagagg acggggaggc
    17761 tgttggggtt aagggttttg taaactaatc aggttgtgca ggctgctttt tgcttttcag
    17821 acgacctgaa atcaacggtt gtaaatccaa tctgtttttc aaaccgcctg caaccgcaaa
    17881 atctgccgca atccgctccc tcccccgtgg gggagggccg gggaggcggc attctccaag
    17941 ttgcggcaac ctttcccaac aacttaaccg cccaaataca agccttgcgg cttgctgccc
    18001 tctctccagc cctctcccac gggagagagg actatgaagc ccgtcggcag gcgcggggct
    18061 tgggacggca ttgctgttgc ggtttcgggc gcggctttat tcgacgacaa ttttagggaa
    18121 ccggctgctg aagtctttgc ctttgtcggc aatgcccagg gcgatttgga atgcggcatc
    18181 ggtgtagatt cgggcgatgg cggggtgttc gtcgaacagt cgcgccggtg tgccgccgtc
    18241 catggcttcg cgcacgggca ggcttagggg aagctgtccg agcaggggga cgttgaggcg
    18301 ggccgccaaa tctttgccgc cgtccgtgcc gaacagtgct tcgctatgcc cgcagttgga
    18361 gcagatgtgg acggacatgt tttccaatac gcctaaaatg ggaatgttga ccttgcggaa
    18421 catatccaca gccttgcgcg cgtctatcag cgcgatgtcc tgcggcgtgg ttacgatgac
    18481 ggacccggtt acggggatgc gttgggacag cgtgagttgg atgtcgcccg tgccgggggg
    18541 caggtcgatg aaaagatagt ccacttcgtc ccactcgctt tggaacatca gctgctgcaa
    18601 ggcttggctg accatcggcc cgcgccagac gacggcttgg ccggtatcga ctagaaagcc
    18661 gatggacata acctgtatgc cgtctgaaga ttcgacggga atcagttttt ggtttttctg
    18721 atcgggtttg cggtcgtgta cgcccagcat cgtgggctgg ctcgggccgt aaaggtcggc
    18781 atcgagcacg ccgacgcgcg cgcccatgcg cgccattgcg gcggcaaggt tggcggtggc
    18841 ggttgatttg cccacgccgc cctttcccga tgcgacggca atgatgtttt tcacaccttt
    18901 gatagtagta acgccgggcc ggactttgtg tgtgccgatt tcggcgtcta tgcccaagtg
    18961 gatgtgcgcg ccgcctgttt cgggcatcag ggtttcttgt acggcatcgg cgagggcggc
    19021 ggcgatgtgc gcgacaggga agccgaaatg cagggcgatg tggatgccgt ctgaacgctg
    19081 tccgaccgaa cggacggcct tttcgccgcc gagcgtgcgt gccgtattcg gaacggcgac
    19141 ggtgtcgagg agggtgcgga tgttttgtat attcatgatg tgtgcctctg ttcgcaagtc
    19201 gtgtgagggg aagatagacg gaaagtgtac atgattttgg gcagtttgct tttttcttgt
    19261 gcgaactttt acacggtatg cggcggattg tgaaaatccg ttgccaaaat atcccgtgca
    19321 aacagcagtc tgcaattaag cctgtaaaat ttgcttgacg gcggggaacg gggctgtata
    19381 atgcacggct tatcgggtcg ttagctcagc cggtagagca gcggactttt aatccgttgg
    19441 tcgagcgttc gaatcgctca cgacccacca gataaccgga agccaagttt cgagacttgg
    19501 cttttttgtt tgcctgccgg ggcgtaccgt ttgcgggggt gtatgaaaaa gaaactgctt
    19561 tcgggcatca aatttgccgt tcggacggca ttggtgtttt tgctggtgtc gctgtttttg
    19621 gattgggtac gcaagcccga cgaacctgcc ggggcggcag ggcggccttt gaccctgctg
    19681 tcggggcagc ggctgacttt gggacagttt agccgggata ggacggtgct ggtgtatttt
    19741 tggggaagct ggtgcggcgt gtgccgttat cagtcgccga taatcgatga tttggcggcg
    19801 gacggcgtgc cggtcgtcgg tgtggcggta cgttccggca gcgcgtccga agtggcggca
    19861 tatatggcaa aacggggctt gggctttccg actgtcagcg atgaggacgg gggtttggca
    19921 aggtcttggc ggattgccgc aacgcctgcc gtcgttttgg tcaaaaatgg gaaaatggtc
    19981 cgctatacga cgggaatcag cagttattgg ggcttgcgcg cacgaatttt tcaggcggat
    20041 tttttcggtt aactttggtt tttgtcaaac tttccgctat gtagggaatc aaacggcata
    20101 tgccttgcgc ggcggatgcg gcggcttttg ttttttggga aagagccggc gcgcctgccg
    20161 atgtaatccc ttgttgtgat tgtgggaaaa atagattaaa atataactat taaaatattt
    20221 tcagatagga ttatcggaat taaagtcttt tatacccggt cgtccgatga ggtttatagc
    20281 gtattgttgc tatatgttcg ttttgttata taacggttgc atcaaaaatt acgcccacag
    20341 gctttcccga cggtttgaaa gtttgatttt cgataacttg gagacttaaa caatgcctac
    20401 ccaatcaaaa catgcgtcta tcaatatcgg tctgatacag gcaagggaag ccctgatgac
    20461 ccaattcagg cctattctga atcaggcgaa tattaccgat cagcaatggc ggattatccg
    20521 tcttttggcg gaaaacggca cattggattt tcaggatttg gcgaatcagg cgtgcatttt
    20581 gcgccccagc ctgaccggta tcctgacccg ccttgaaaaa gcggggttgg tcgtccgcct
    20641 gaaaccttcc aacgaccaac ggcgcgttta tctgaagctg acttccgagg gcgagaagct
    20701 gtatgaggaa atcggcgaag aagtggacga gcgttacgac gctatcgagg aagtgctggg
    20761 ccgcgagaaa atgctgctgc ttaaagacct gttggcagaa cttgccaaaa tcgaggatgc
    20821 gttgaactcg taatacgccg taatgcgcgg aaacgtccga ccgacggctt tttgaatcag
    20881 aactgctgca catgggggat gccttgtgtg cagcattctt atatagggga cggtttaaag
    20941 gggaaaaatg gcggatttgc agaaaacttt tcaaacttcg ttccgtgatg cgatggcatc
    21001 ctgtgcggca ggcgttcatg tcatcacgac agacggtgcg gcagggcgtt acggcattac
    21061 aatgacggcg gttgcgccgg ttaccgacga gccgccgacc gtgatgctgt gcataaaccg
    21121 gagtgcgcga atcattccga tcctgtcgga aaacggcagc ctctgcatca atatgctggc
    21181 ggacgaacat caggatgttg ccgaacattt tgccgggctg accggcctgt cgcccgaaga
    21241 gcggtttgcc taccatattt ggcatcgcgg caaaacggga caacttgaaa tagaaggcgc
    21301 gttggcgcac ctgcacgggc atattgtcgg caaacatgaa atcggcacgc attttgtgtt
    21361 ttacgtcagg ctcgacgaaa tcaaaaactg cgggtgcaaa cgccccgcgc tgctgtattt
    21421 cagacggcag tttaggcctt tagactgata ttcggacaga tatatgaaag cgatgatact
    21481 ggcggcagga cgcggcgagc gtatgcgccc tttgaccgac accactccga agccgctgct
    21541 cgatgtggcg ggtaagcctc taatcggttg gcacttgtgc cgtctgaagc aggcggggtt
    21601 taccgaaatc gtcatcaacc acgcttggct gggtcggcag atagaaaatg ctttgggcga
    21661 cggctcggct tatggcgtga acatcgccta ttcgcccgaa cccgcaggcg gtttggaaac
    21721 ggcaggcggc atcgcgcagg cattgccgct gttgggtggg cagccgtttt tggtggccaa
    21781 cggcgacgtg ctgaccgaca tcgattttac cgccgcgttt cagacggcat cgtccctgcc
    21841 cgggcacatt tccgcacatt tgtggctggt gggaaatccc ccgcacaacc ccgacggcga
    21901 tttttccctg ctgcccgacg gcagcgtgcg gccggaagta tccggcggca acggactgac
    21961 attcagcggc gtgggtattt accgtcctga aatgtttgac ggaatcgaag cgggcagtgt
    22021 ggcaaaactc gcgcccgtat tgctgaacga aatgcggcaa aaccgcgtga gcggtcagaa
    22081 gcatacgggc ctgtggctgg atgtcggcac ggtatgccgt ctgaaggaag cgcaagccct
    22141 tgcagcggct tggaagtgaa aactcggttt caggcggtat ggcggattcg gtttaacgtt
    22201 tcagtgccaa cgtcaacacg ccgccgtaac cagccccaag tctatccatt cctgcgtgtt
    22261 cgggcgttcg tctaagaaaa tcaccgccat cagcgcgacc aagaccaggc tgaatttgtc
    22321 gacgggcgcg acttgcgggg ctttgcccag ttgcagggct ttgaagtagg cgagccaaga
    22381 tgcgccggtg gcgagtccgg acaaaatcca gaaacgtccg gttgcgcccc gtgaagccgt
    22441 ttacgccctg ccatttgccg gtgtaggtca aaaacaatac caaggcggcg aggatgacca
    22501 aggtgcggat aaaggcggcg aaatccgaat ctatgccctg caagcccatt ttggcgaaaa
    22561 cggcggtcaa tgaggcgaag cctgccgatg ccaatgccca aaacagccat gcgttgctgc
    22621 ccatgttttc tcctttgatt gtgaacaata tgaacggtat ttttgttgct gcgtcaaaaa
    22681 tttcactgcg ggtttggtgc ggataacgtt ataatatgcc tgatattatt ttcaatccac
    22741 ctgtttgtcg cctgatgctt tcagacggca tgtccctcct catttctaaa ggaaaatcat
    22801 gagcttcaaa accgatgccg aaaccgccca atcctccacc atgcgcccga ttggcgaaat
    22861 tgccgccaag ctgggtttga acgttgacaa cattgagcct tacggtcatt acaaagccaa
    22921 aatcaatcct gccgaagcgt tcaagctgcc gcaaaaacaa ggcaggctga ttttggttac
    22981 cgccatcaac ccgactccgg cgggcgaagg caaaaccacc gtaaccatcg gtttggcgga
    23041 cgcattgcgc catatcggca aagactctgt gattgctttg cgcgagcctt ctttgggtcc
    23101 ggtgttcggc gtgaaaggcg gcgcggcagg cggcggctac gcgcaagttt tgccgatgga
    23161 agacatcaac ctgcacttca ccggcgactt ccacgccatc ggtgcggcga ataacctcct
    23221 cgccgccatg ctcgacaacc atatctacca aggtaacgag ttgaacatcg accccaaacg
    23281 cgtgctgtgg cggcgcgtgg tcgatatgaa cgaccgccag ttgcgcaaca tcatcgacgg
    23341 tatgggcaag cctgttgacg gcgtgatgcg tcccgacggc ttcgacatca ccgtcgcctc
    23401 cgaagtgatg gcggtattct gccttgccaa agacatcagc gatttgaaag agcgttttgg
    23461 caatattctc gtcgcctacg ccaaagacgg cagccccgtt tacgccaaag atttgaaggc
    23521 acacggcgcg atggcggcat tgctaaaaga tgcgattaag cccaatttgg tgcaaaccat
    23581 cgaaggcact ccggcctttg tacacggcgg cccgttcgcc aacatcgccc acggctgcaa
    23641 ctccgttacc gcaacccgtc tggcgaaaca ccttgccgat tacgccgtaa ccgaagcagg
    23701 cttcggcgcg gacttgggtg cggaaaaatt ctgcgacatc aaatgccgcc ttgccggttt
    23761 gaaacctgat gcggcagtcg tcgtggcgac tgtccgcgcc ctgaaataca acggcggcgt
    23821 ggaacgcgcc aaccttggtg aagaaaacct cgaagccttg gcaaaaggtt tgcccaacct
    23881 gttgaaacac atttccaacc tgaaaaacgt attcggactg cccgtcgtcg ttgcgctcaa
    23941 ccgcttcgtg tccgactccg atgccgagtt ggcgatgatt gaaaaagcct gtgccgaaca
    24001 cggcgttgaa gtttccctga ccgaagtgtg gggcaaaggc ggcgcgggcg gcgcggattt
    24061 ggcgcgcaaa gtcgtcaatg ccatcgacaa ccaacctaat aacttcggtt tcgcctacga
    24121 tgtcgagttg ggcatcaaag acaaaatccg tgcgattgcc caaaaagtgt acggcgcgga
    24181 agatgtcgat ttcagcgcgg aagcgtctgc cgaaatcgcc tcgctggaaa aactgggctt
    24241 ggacaaaatg ccgatctgca tggcgaaaac ccaatattca ttgagcgaca acgccaaact
    24301 cttgggctgc cccgaaggct tccgcatcac cgtacgcggt atcactgttt ccgccggcgc
    24361 gggcttcatc gttgcgttgt gcggcaatat gatgaaaatg ccgggcctgc cgaaagttcc
    24421 ggctgccgag aaaatcgatg tggacgaaca cggcgtgatt cacggcttgt tctgaacggt
    24481 ttttgaaacc ggatgccgtc tgaagccgtt tcagacggca ttttttcgga acgcgggcgg
    24541 cggtatgcta taatccgccg ttaaatttct ctattttcag gaaaaaccat gagtttgaaa
    24601 tgcggcatcg tcggtttgcc caacgtcggc aaatccaccc tttttaacgc gctgacccaa
    24661 tcgggcatcg aagcggcaaa ctatcccttc tgcaccatcg aacccaacgt cggcatcgtc
    24721 gaagtgcccg acccgcgtat ggcggagctg gcgaaaatcg tcaatccgca aaaaatgcag
    24781 cccgccatcg tcgagtttgt cgatattgcc ggcttggttg cgggcgcgag caaaggcgaa
    24841 ggcttgggca accggttcct tgccaacatc cgtgaaaccg atgccatcgt caacgtcgtg
    24901 cgctgctttg acgacgacaa catcgtccac gtttccggca aagtcgatcc gattgccgac
    24961 atcgaaacca tcggcaccga attggcgctt gccgacttgg caagtgtgga aaaagccatc
    25021 gtccgcgaag aaaaacgcgc ccgatcaggc gacaaagacg cgcaaaaact agtcgatttg
    25081 tgcaaaaaac tgctgccgca tctggacgaa ggcaaacccg tgcgttcctt cggtttggac
    25141 gcggaagaac gcgcgctgct gaagccgctg ttcctgctga ccgccaaacc tgcgatgtat
    25201 gtgggaaacg ttgccgaaga cggttttgaa aacaacccgc acctcgaccg cctgaaagaa
    25261 ttggcggcaa aagaaaacgc ccccgtcgtt gccgtctgcg ccgcgatgga gagcgaaatc
    25321 gccgaattgg aagacggtga aaaagccgaa tttctcgccg aaatgggctt ggaagaaccg
    25381 ggcttgaacc gcctcatccg cgcgggttac gaccttttgg gactgcaaac ctacttcacc
    25441 gccggcgtga aagaagtccg cgcgtggacg atacacaaag gcgacaccgc gccgcaagcc
    25501 gccggcgtga tccatacgga ttttgaacgc ggcttcatcc gcgcccaagt gattgcctac
    25561 gatgattttg tctcgctcgg cggcgaagcc aaagccaaag aagccggcaa aatgcgcgtg
    25621 gaaggcaagg aatacgtcgt gcaggacggc gacgtgatgc actttttgtt taacgtgtaa
    25681 cccaaatgcg gcaggttttc agacggcttt gccggaaatg ccgtctgaag ccggttttgg
    25741 tggttttcga cgttcccata ccgccggaat gcagccgcat caaaataaaa tcccgcccgc
    25801 atttccgatt tgccctcccc gattcctgca aaacaaaccg cctgccctgc cgttacggga
    25861 agccgtccgg tattccgaat atcccgaacc ccgatacaaa atgacctttc agacggcatt
    25921 tgcacagccc gccgcgtttc aagtaaaaac attatgagcc aagccttacc ctaccgcccg
    25981 gacatcgaca cattgcgcgc cgccgccgtc ttgtccgtca tcgtgttcca tatcgaaaag
    26041 gattggctgc cgggcgggtt tctcggtgtc gatatattct ttgtgatttc aggctttttg
    26101 atgacggcga tcctccttcg cgaaatgtcc ggggggcgtt tcttcctcaa gacattttat
    26161 atccgccgca tcaaacggat tttgcccgca tttttcgccg tattggcggc aacgctggca
    26221 ggcggcttct ttttattcac caaagatgat ttctttcttt tgtggaaatc cgcgctgacc
    26281 gccttgggtt tcgcctccaa cctgtatttt gcaaggggga aggattattt cgatcccgcg
    26341 caggaagaaa agcccctgct gcacatctgg tctttgtcgg tcgaagaaca attttacttt
    26401 gtctttccga tattgctgtt gcttgtcgcc cgcaaaagcc tgcgcgtaca gttcggcttc
    26461 ctcgccgcat tgtgcgcctt aagccttgcc gcttccttta tgccttccgc gctcgataaa
    26521 tattacctgc cccacctgcg cgcctgcgaa atgctggtcg gatcgctgac cgccgtgcgg
    26581 atgcggtacc ggcaacagcg gaatcccgcc gtcgggaaac ggtatgccgc cgtcggcgca
    26641 ttgttttccg cgtgcatact gtccgcctgc ctgtttgcct attcggaaca aaccgcctat
    26701 ttcccgggcc ccgccgcttt gattccctgt ctggctgttg ccgcgctgat ttatttcaac
    26761 cattacgaac acccgcttaa aaaatttttc caatggaaaa tcaccgttgc cgccggtttg
    26821 atttcctatt cgctttatct gtggcattgg ccgatattgg cctttatgcg ctatatcggc
    26881 ccggacaacc tgccgcctta ttcgccggcg gcagcgatcg tcctgaccct ggcgttttcc
    26941 ctgatttctt atcactgcat cgaaaagccg tttaaaaaat ggaaaggctc gttcgcacaa
    27001 tccgttttat ggatttatgc cttgcctatg ctcgttttgg gcgcgggctc gtttttcgcg
    27061 atgaggctgc cgtttatggc gcaatacgac cgcttggggc tgacgcgttc caacacctcc
    27121 tgccacaaca ataccggcaa acaatgcctg tggggggata cggaaaaaca gccggaactg
    27181 ctggttttgg gcgactccca cgccgaccat tacaaaacat tcttcgatgc cgtgggcaaa
    27241 aaagaaaaat ggtccgccac tatggtttcc gccgacgcct gcgcctatgt ggaaggctac
    27301 gcgtcccgtg tgttccaaaa ctgggccgcc tgccgcgccg tttaccgcta tgccgaagaa
    27361 cacctgcccc ggtatccgaa agtggttttg gcgatgcgct ggggcagcca gatgcccgaa
    27421 aacagccgct cccttgccta cgatgccggt tttttccaaa aattcgaccg tatgctgcac
    27481 aaactctcat ccgaaaaaca agccgtttac ctgatggcgg acaacttggc ttcgtcttac
    27541 aacgtccagc gcgcctatat cttgtcttca cgcataccgg gttgccgcca aacactgcgc
    27601 ccggacgacg aaagcaccct gaaagccaat gcccgcatca gggaattggc agccaaatac
    27661 cccaacgtct atattattga tgccgccgcc tatatccccg cagatttcca aatcggcgga
    27721 ttgccggttt actcggacaa agaccacatc aacccttacg gcggcacaga attggcgaag
    27781 cgtttttccg aaaaacaaag gtttctcgat acgcgccata accattgatt cgcttaaatt
    27841 tgttacaatc ggcggtttgc aaaaccgcta atttttttga aagagaccga tgagcgtcat
    27901 ccaagacctg caatcgcgcg gccttatcgc gcaaaccacc gacatcgaag ccttagacgc
    27961 tttgctgaac gaacaaaaaa tcgcccttta ctgcggtttc gacccgacag ccgacagcct
    28021 gcacatcgga cacctgctgc ccgtattggc attgcgccgc ttccaacagg cggggcatac
    28081 gccgattgca ctggtgggcg gcgcgaccgg tatgatcggc gaccccagct tcaaagccgc
    28141 cgaacgcagc ttgaattccg ccgaaaccgt tgccggctgg gtaggaagca tacgcagcca
    28201 attaacccct ttcttgagct ttgaaggcgg aaacgccgcc attatggcga acaatgccga
    28261 ctggttcggc agcatgaact gcctcgactt cctgcgcgac atcggcaagc atttctccgt
    28321 caacgccatg ctgaacaaag aatccgtcaa acagcgcatc gaccgcgacg gcgcaggcat
    28381 ttccttcacc gagttcgcct attccctgct gcaaggctac gacttcgccg agttgaacaa
    28441 acgccacggc gcggttttgg aaatcggcgg ttccgaccag tggggcaaca tcaccgccgg
    28501 tatcgacctg acccgccgcc tgaaccaaaa acaagtgttc ggtctgaccc tgcctttggt
    28561 taccaaatcc gacggtacca aattcggcaa aaccgaaggc ggcgcggtgt ggctgaacgc
    28621 gaaaaaaacc tcgccgtacc agttctacca gttctggctg aaagtcgccg atgccgatgt
    28681 gtataaattc ctgaaatact ttaccttcct gtccatcgaa gaaatcggtg tcatcgaagc
    28741 caaagacaag gcaagcggca gcaagcccga agcgcaacgc atcctcgccg aagaaatgac
    28801 ccgcctgatt cacggcgaag aagcccttgc cgccgcgcaa cgcatttccg aaagcctgtt
    28861 tgccgaagac caaagccggc ttaccgaaag cgacttcgag cagctcgccc tcgacggcct
    28921 gcctgcattt gaagtttcag acggcatcaa cgccgtcgaa gccttggtca aaaccggctt
    28981 ggcagcgtcc aacaaagaag cgcgcggctt tgtcaacgcc aaagcggttc tgctcaacgg
    29041 caaaccggct gaagccaaca accccaacca cgccgccgaa cgccccgacg atgcctatct
    29101 gttgataggc gaatacaaac gtttcggcaa atacaccatc ctccggcgcg gcaaacgcaa
    29161 ccacgcgctt ttggtttgga aataatccga ttgccgcaga aatgccgtct gaagctttca
    29221 gacggcattt ttatcaaatg caaaacaccc tgcgcctgcc gatatgtcgt catttccatg
    29281 caggcgggaa ttcaaacttg tccgcacgga aacttatcgg gcaaaacggt ttcttcagtt
    29341 ctacgttcta gatgcccgct ttcgtgggaa tgacggttca gttgctacgg ttactgtcag
    29401 gtttcggtta tgttggaatt tcgggaaact tatgaattgt cattcccgcg aaagtgggaa
    29461 tccaggactc aaaatctcaa gaaaccgttt tgcccgataa gttcctgcac tgacagatct
    29521 agattcccgc ctgcgcggga atgacgggtt tcgagattgc ggtgttgtcg ggaatgatgg
    29581 aaaatggcgg gaattatgta aaaaatgccg tctgaaaccg ttggaagcat cgtaaacgtt
    29641 ggagtcgatg aatcggtggg cttcagtccg ccattcccat caatccaaca tttctaccgt
    29701 tttcatcgaa tccatcgaat ccgccccttt cgacaatctg accctatgca accgaaccgt
    29761 cattcccacg gaagtgggaa tctagaatct cggggtttca gtcatttccg atagattccc
    29821 gccgcgtcgg gggtccggat tcccgcctgc gcgggaatga cgaatttcga gattgcggtg
    29881 ttgtcgggaa tgacggttcg ggtatttcca cgcccgcccc gcgcctgtaa acggcgggcg
    29941 attaaaaatg ccgtctgaag gttcagacgg cattggaatc gggggatcag aagcggtagc
    30001 gcacgcccaa tgaggcttcg tgggttttga agcgggtgtt ttccaagcgt ccccagttgt
    30061 ggtagcggta gccggcgtcc aaggtcaggt tgggcgtgat gtcgatgcct acgcctgcca
    30121 ctgcgccgaa gcccaagctg ctgatgctgc ggctttcgtg atgggcaggt tttttggtcg
    30181 tagctcctgg gataggactt ggctgaccac tctgtgggta agtggtaaca gtcgtggttt
    30241 ctttttccat tgaatgaacc tgatgtctga cgtgtccgta ggcgacgcgc gcgccgatat
    30301 agggtttgaa tttatcgttt atttggaaat cgtaaatagc ggataagccg agtgaggaga
    30361 cggcgtggaa cgtaccgttt tcctgatttt ccgtcttccg gtctatcctg ttgccattgt
    30421 cgtgtttttt cacattttct atgttaacgg aatatttatt gttgttccac tttctgtaac
    30481 gggcataatc tgccgctatc ctccagccgc cgaagtcgta gccgaccgac acccgggggt
    30541 ggatggaatg cgtacggatg tttctgaaat aatcgcttac cgtgcttatt ttgtttttgc
    30601 ctgtaccggt tggtttcgga taatcgtggg taatgcgttc ggcggcgtag gctaaatccg
    30661 cctgcacata cgggccgcgg ccattgcctt cacttgccgc ctgcgctgcg gaagagaaga
    30721 gaagagaaga gaagagaaga gaagagaaga gaagagaaga gaagagaaga gaagagaagg
    30781 ttttttgcgg gctggattca ttttcggctc cttattcggt ttgaccggtt aaaaaaaaga
    30841 ttttcactga tgttgaaggg cggattatat cgggttccgg gcggtgtttc aatacatagc
    30901 accgcgcctg ctgcgcgttt tatgcgtttg gcgcgttcgg cggcgggaaa tttgcctact
    30961 tttcccgcgt cgggcgggcg gaacgggcgg cacactgtct ataaaccgca ataccgttta
    31021 caatgaccgc ctgtttcacc acatacccga acgcaacaat gtttcaacac acgggacggc
    31081 acatcaagca ccgccctatg tgtcgtcctg atttggaagg ggttgcaccc ctcccgaata
    31141 aagtctgatc ctgccgcccc gaaggacgga tgtccgagtg gcggggtttc aaccgaaaag
    31201 gaaatacaat gaaaatcagg ccggggcggc acaacgcgcc cgactttccg cacggggccg
    31261 ccgtaaccat aggcaatttc gacggcgtgc acctcggaca caaacacatc ctccaaaaac
    31321 tccgcctcga agccgacgca cgcggattgc ccgtcgttgc cgtcattttc gagccgcaac
    31381 ccaaagaatt ttttgccgcc cgcacaggca aaaccccgcc gtgccgtatc agccccctgc
    31441 gtaccaaact cgggctgctc gaaaaaaccg gttgcgtcga tgccgcctgg gttttgcgtt
    31501 tcgatcggaa tttttccgaa atatccgcgc aagcatttat cgaccgcctg ctgcgtcaaa
    31561 ccttgaatac gcgctatttg ctcgtcggcg atgatttccg tttcggtgcg gggcgggaag
    31621 gctgttttga acttttggca caacagcccg atatgcaaac cgagcgcacg ccttccgtca
    31681 tcgtcgaaga catccgcacc agcagtaccg ccgtccgcca agccctttca gacggcaacc
    31741 ttgcctatgc gaagaaactt ttggggcacg actacgtttt ggggggcagg gtggtgcacg
    31801 gcagaaaact cggacgcacc ttaaacgccc cgaccgccaa tatccgcctg cccggccacc
    31861 gttatgcact cggcggcgtg ttcgtcgtcg aggcggacgg cgcattcggc acgcggcgcg
    31921 gcgtggcgag cttcggcttc aatcccaccg ttgatggcgg ctgttctcaa aagcttgaag
    31981 tccacctgtt cgactttcaa ggcgatttgt acggacaacg gttgaacgtc cgcttcctgc
    32041 acaaactgcg cgacgaggaa aagtttgacg gtatggaaga actgaaaagg cggattgaag
    32101 ccgatatgga agccgcaaag tgttggtaga aaaaccttat acaaaccatc cgattgggct
    32161 acaatcagcc ttttaactgt tcagacggca caggggtttc ccgttgtgaa atactgtttg
    32221 aggcgcaatg ccgtctgaaa ccgaaatatt gtaacaatag agattaaaaa atgaccgatt
    32281 acagtaaaac cgtcaacctg ctcgaaagcc cgtttccgat gcgcggcaat cttgccaagc
    32341 gcgagcctgc gtggctgaaa agctggtacg agcaaaaacg ttaccaaaaa ctgcgcgaaa
    32401 tcgccaaagg ccgtccgaaa ttcatcctgc acgacggccc gccgtatgcc aacggcgaca
    32461 tccatatcgg tcatgccgtc aataaaatcc ttaaagacat tattatccgc agcaaaaccc
    32521 aagccggttt tgacgcgcct tatgtaccgg gttgggactg ccacggcctg cccatcgaag
    32581 tgatggtgga aaaactgcac ggcaaagata tgcctaaagc ccgtttccgc gaattgtgcc
    32641 gcgaatatgc cgccgaacag attgcccgtc agaaaaaaga ctttatccgc ttgggcgtgt
    32701 tgggcgattg ggatcatcct tacctgacta tggatttcaa aaccgaagcc gataccgtgc
    32761 gtatgctcgg cgaaatctac aaatccggct atctctaccg tggtgcgaaa ccggttcagt
    32821 tttgcttgga ttgcggatct tcgctggcgg aagcggaagt ggaatacaaa gacaaagtat
    32881 cgcctgcgat tgacgttgcc tatccgttta aagacactgc cgcgcttgcc gccgcattcg
    32941 gcttggcagg tatcgaaggc aaagcgtttg ccgtcatttg gacgaccacg ccttggactc
    33001 tgcctgcgag ccaggccgtg tctgccggcg cggacgtggt gtatcaatta atcgatacgc
    33061 ccaaaggcaa attggtgctg gcgaaagatt tggcggaagg cgctttgaaa cgctacggct
    33121 tttcagacgg catcgccatc cttgccgaaa ccaccggcga caagctggaa aacctgcaca
    33181 tgaatcatcc gttcctcgaa cgcgatattc ccatgctcaa cggcgaacac gttaccaccg
    33241 atgccggtac cggcttggtg catactgcgc ctgcgcacgg tttggaagac tacgccgtct
    33301 gcaataaata cggcatcgag ctttacaacc ctgtcaacgc cgaaggcaaa tacataagcg
    33361 aaacgcctcg tgtcgcaggc atgagcgttt gggaggcgaa tcccgtcatc ctgcaatggc
    33421 cggaagaaac cggcaacctc ttggcaagca gcaaaatcga acacagctac gcccactgct
    33481 ggcgccacaa aaccccgctg atttaccgag cgacaggtca gtggtttgtc ggcatggaca
    33541 aagccggcag cgacggtaaa accctgcgcg acaaagccat caaagccgtg gacgacaccg
    33601 aattcttccc gccatggggt cgtgcgcgtt tggaatccat gattgaaggc cgtcctgact
    33661 gggtggtttc acgccaacgc tattggggca cgccgatgac tttctttgtt cacaaagaaa
    33721 cgggcgagct gcatccgaac tctgccgaac ttttggaaaa agtcgcgcaa cgcatcgaag
    33781 aaaaaggcat cgaggcttgg ttctccctcg ataaaagcga attattaagc gcggaagatt
    33841 gcgaacatta cgacaaactc cccgatacca tggacgtatg gttcgactca ggctcgacgc
    33901 attattccgt tgtaaaacaa cgcgaagaat tggaatggcc ggctgacttg tacctcgaag
    33961 gcagcgacca acaccgcggc tggttccaat cctctatgct gaccggttgc gcctcatcca
    34021 tgggacgcgc accgtataaa cagctgctga cccacggttt cgtggttgac caaaacggcc
    34081 gcaaaatgtc gaaatccatc ggcaacgtcg tcgcgccgca ggaagtctat aacgaattcg
    34141 gcgcggacat cctgcgcctg tgggcggcat ccaccgatta cagcggcgaa ttggcgattt
    34201 ccaaagaaat cctcaaacgc gtaaccgaaa gctaccgccg catccgcaat accttgagct
    34261 tcctgtttgc caacttgagc gatttcaacc cgattgaaga tgccgtgcaa caggcggata
    34321 tggtggaaat cgaccgctac gccttggtat tggcgcggcg gctgcaagag cgtttggcag
    34381 gcggttacta tccgcgctat gccttccact tcgccgtgaa agacattgtt tctttctgct
    34441 cggaagactt gggcgcgttc tacctcgaca tcctgaaaga ccgcctctac accaccaaag
    34501 cccacagccg cgcccgccgc agcgcgcaaa ccgccctgta ccacatcacg cgcagcctgg
    34561 ttctcttgat tgcaccgatt ttgtgcttca ccggcgaaga agcgtgggac atcatcggcg
    34621 gcggcgaaga agacagcgtc ctcttccata cttggcacga gttcccggcc atcaacgaaa
    34681 aagccgaagc cgaactggtg aaaaaatgga cggcaatccg cgaagcacgc gaagcagtaa
    34741 ccgccgccat cgagcctttg cgcgccgaca aaaccgtcgg ttcgtccttg caggcggaag
    34801 ccgaaatcac cgcgccggaa gaaatggccg gctatctgaa tgctttgggc gaagaattgc
    34861 gctttgccct gctggtgtct aaagcagaag tgaaagtcgg tgatgaactt gccgttgccg
    34921 ccaaagccag cgacggcgaa aaatgcgaac gctgctggca ttacacccgc gatgtgggcg
    34981 cggttgcagg ttatgaaacc gtctgcaaac gctgtgcgga gaatgtcggc ggagaaggcg
    35041 aaacgcgcca ttacgcctga taaagtttga gcaaatgccg tctgaaaccg ccaaacagta
    35101 tttcagacgg cattttttgt gccgcgattt gtcttcataa tggcggaggg gttttaagat
    35161 tacggtattg tcgggaatga cggttcagtt gctacggtta ctgtcaggtt tcggttatgt
    35221 tggaatttcg ggaaacttat gaattgtcat tcccgcgaaa gtgggaatct agaatctcgg
    35281 ggtttcagtc atttccgata gattcccgcc gcgtcggggg tccggattcc cgcctgcgcg
    35341 ggaatgacgg gtttcgagat tgcggtgttg tcgggaatga cggttcgggt attttactgc
    35401 gcccgccccg cgcctgtaaa cggcgggcgc atcaaaaatg ccgtctgaag gttcagacgg
    35461 catcggtgtc ggggaatcag aagcggtagc gcacgcccaa cgaggcttcg tgggttttga
    35521 agcgggtgtt ttccaagcgt ccccagttgt ggtagcggta gccggcgtcc aaggtcagct
    35581 tgggcgtgat gtcgaaaccg acaccggcga tgacgccgag acctacgcgg cggatgctgt
    35641 cgctttggtg atgggcgttt tgcgactgtg gactaatatt ataagttgta gattttatgt
    35701 tcttaccatg ggggacggta agaatatcta t
//
LOCUS       DAXFWM010000015        31363 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000015 DAXFWM010000000
VERSION     DAXFWM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31363
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.015"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..823)
                     /locus_tag="ACFI8B_000696"
     CDS             complement(<1..823)
                     /locus_tag="ACFI8B_000696"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3833976.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVEQSQDFICGFTNDEKSILNPQ"
     gene            complement(873..1103)
                     /locus_tag="ACFI8B_000697"
                     /pseudo
     CDS             complement(873..1103)
                     /locus_tag="ACFI8B_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(1153..4157)
                     /locus_tag="ACFI8B_000698"
                     /pseudo
     CDS             complement(1153..4157)
                     /locus_tag="ACFI8B_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(4277..5587)
                     /locus_tag="ACFI8B_000699"
     CDS             complement(4277..5587)
                     /locus_tag="ACFI8B_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC3833977.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            5784..6085
                     /locus_tag="ACFI8B_000700"
                     /pseudo
     CDS             5784..6085
                     /locus_tag="ACFI8B_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714068.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6765..9509
                     /locus_tag="ACFI8B_000701"
     CDS             6765..9509
                     /locus_tag="ACFI8B_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3833978.1"
                     /translation="MPGKKNTPIIRQNHTGWGVQSTIDSDVVLSAANNIVEVGGSPMN
                     QEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTGARPGTRDDGNKIILEA
                     GGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAYGDKSLVKLIGENNIVK
                     SEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIWGLYSNNSSISLKGKNN
                     VISNPKYNVFAYKKAKVDLTVENKNTLSDAEFGVYALNTSMVNLSSKDNNEVKSTQVG
                     LYSQDGGSINVDRKDNIIEGDAVALVGKGGSQNIRASRTNLISSKSLGIHAEQAAKIA
                     ITGASNTIHASNAAIRSLDKSEVKIDGQITIDSNVANLARQDGSIHLNYKDDTRITGA
                     TVSDKGLVAIKPLNNTNIVADTIHYKGDVLAVNKGKVELDFTPNIRLVGRLDNFSGLT
                     DSKHKNLFENYVANLDSKSAGEINFNLAKDALWTMTGQSWLDKLEGQGTIDFNNDAKT
                     SGRALHIGELAGANKFLMHLNKDGIHSDMLYVKKGTSTPQEVVVKNLSEVLDSMNYGE
                     RLRFATVTNSKNEFVNGKKYIDDTHLMEDALTVEYSAHNGDKNNKDDYNKSFNGSEMT
                     AEKAGDDYVNKTYTDNRQNVYLVKQATGNPSRNVKNINDMFDSTAHYAFTLDTYAKRE
                     GERAFSTLDKKEGDWIRLTHTRVIQSNAFRFHNNDFEIGYDRFSLNEQEKKRKWGISL
                     DYGHGRTSLWNTFGKDKIRKYELALYNTTQYIDKEGDETGYIDNVLKIGKLRNRVIAR
                     NHMGQLWGKGKYSNTLFSISTEYGRRKFLDDDKLWRITPQVQLQYSYLRGTGYRIDNG
                     INVNLSHANSLIGRLGLDVVRKFDGGKKLFYIKGNIFHEFLGSRSFKAFEGKSHYAQK
                     "
     gene            9822..10016
                     /locus_tag="ACFI8B_000702"
                     /pseudo
     CDS             9822..10016
                     /locus_tag="ACFI8B_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063514830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(10037..10953)
                     /locus_tag="ACFI8B_000703"
                     /pseudo
     CDS             complement(10037..10953)
                     /locus_tag="ACFI8B_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11208..12672
                     /locus_tag="ACFI8B_000704"
                     /pseudo
     CDS             11208..12672
                     /locus_tag="ACFI8B_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            12923..13291
                     /locus_tag="ACFI8B_000705"
     CDS             12923..13291
                     /locus_tag="ACFI8B_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833979.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGRIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            13364..14773
                     /gene="thrC"
                     /locus_tag="ACFI8B_000706"
     CDS             13364..14773
                     /gene="thrC"
                     /locus_tag="ACFI8B_000706"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC3833980.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            14820..15614
                     /locus_tag="ACFI8B_000707"
     CDS             14820..15614
                     /locus_tag="ACFI8B_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833981.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLALRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            15802..16578
                     /locus_tag="ACFI8B_000708"
     CDS             15802..16578
                     /locus_tag="ACFI8B_000708"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC3833982.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(17234..19705)
                     /locus_tag="ACFI8B_000709"
     CDS             complement(17234..19705)
                     /locus_tag="ACFI8B_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC3833983.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALHDLKNRRVGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQVIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKGSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(19702..20880)
                     /gene="hflX"
                     /locus_tag="ACFI8B_000710"
     CDS             complement(19702..20880)
                     /gene="hflX"
                     /locus_tag="ACFI8B_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC3833984.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(20886..22166)
                     /locus_tag="ACFI8B_000711"
     CDS             complement(20886..22166)
                     /locus_tag="ACFI8B_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC3833985.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            complement(22318..23043)
                     /locus_tag="ACFI8B_000712"
     CDS             complement(22318..23043)
                     /locus_tag="ACFI8B_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC3833986.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            complement(23116..23793)
                     /gene="radC"
                     /locus_tag="ACFI8B_000713"
     CDS             complement(23116..23793)
                     /gene="radC"
                     /locus_tag="ACFI8B_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC3833987.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            23820..23942
                     /locus_tag="ACFI8B_000714"
     CDS             23820..23942
                     /locus_tag="ACFI8B_000714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833988.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(23916..25265)
                     /gene="gshA"
                     /locus_tag="ACFI8B_000715"
     CDS             complement(23916..25265)
                     /gene="gshA"
                     /locus_tag="ACFI8B_000715"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC3833989.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            25517..26926
                     /gene="leuC"
                     /locus_tag="ACFI8B_000716"
     CDS             25517..26926
                     /gene="leuC"
                     /locus_tag="ACFI8B_000716"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC3833990.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAVAAAVTGHFTDIRTMA"
     gene            27021..27275
                     /locus_tag="ACFI8B_000717"
     CDS             27021..27275
                     /locus_tag="ACFI8B_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3833991.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            27337..27978
                     /gene="leuD"
                     /locus_tag="ACFI8B_000718"
     CDS             27337..27978
                     /gene="leuD"
                     /locus_tag="ACFI8B_000718"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC3833992.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            28145..29416
                     /locus_tag="ACFI8B_000719"
     CDS             28145..29416
                     /locus_tag="ACFI8B_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3833993.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            29432..30163
                     /locus_tag="ACFI8B_000720"
     CDS             29432..30163
                     /locus_tag="ACFI8B_000720"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC3833994.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKGRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            30197..31267
                     /gene="leuB"
                     /locus_tag="ACFI8B_000721"
     CDS             30197..31267
                     /gene="leuB"
                     /locus_tag="ACFI8B_000721"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC3833995.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNTAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            complement(31295..>31363)
                     /locus_tag="ACFI8B_000722"
     CDS             complement(31295..>31363)
                     /locus_tag="ACFI8B_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3833996.1"
                     /translation="EFRFNFGTPSRQLKILRDRCGI"
BASE COUNT         7761 a         7751 c         8125 g         7726 t
ORIGIN      
        1 cttgtgggtt taatatagat ttttcatcat tggtaaatcc acaaataaaa tcttgacttt
       61 gctcaacaac aggatatttt ccaaaatcct taatatcttt atttaatatt tttgtagtat
      121 ttgggatttt tgctaagcaa gattgtaatt ctatacttcc ccaccccttc ggaatttccc
      181 gtttcaaggt ttcgtcaaac accatgtcgc cgcctgagga tttgtagggc ttgccgttgg
      241 cgtcggggaa atcgaactgc acaaaccagt agtcgtacag ggttttcgcc atctcttcca
      301 ggcgcgcgtt gatttgtttg ttcagggcga tttttttgtc caaagcggat aggacggcgg
      361 cgatggattg ttggatttga ggttctggaa taggcaactc cagtgttttt aatgcatttt
      421 cattgacacg ttgcctgccg gaagttcctt ccatacattc aatcgctcgt tttctaaaat
      481 cgggactgat tgcaaaataa tagagaaatt cgggattggt ttcatttttt gctcttaaca
      541 cgataaattc ggtagaaccg aaagccactt ctccatcatc taaaatatct acaaaagcgg
      601 ttttcccatt ttccaaacac ggggtaatct tagctaataa ggtatcacca ttgcgaaact
      661 tagctccacc gttaaatgcc ttgatttcgt aacccgtaat ttgtcgttga aattctttca
      721 acattgccat cggaacactt tttgccaatg ctcccttggc tagttgctct ctcggattaa
      781 aatcagcaat attttgaatt tgttgtttct taaaatgatt catgatgttc cgttacaaaa
      841 ctcttcaaag gccgtctgaa aaaaacattt catcggtagt tcttgccgtc tgcggcagtt
      901 gtaaactgaa agtttacaac tgaatcgtcc agttcttgtt ctgcaaatat ggctttgatg
      961 tgtttaccga tgttttgttt gctggtttgg tagagttcgg caatttcttt ttgggtaagc
     1021 catagttcgc cgtcaagctc gcgcagggta aattcggata gtccgtcttc cgtggtgtag
     1081 atgatgatgt cgttgttgtg catagctttc ctgtatttat aaatggttaa ggctacctaa
     1141 aaaacctctt tctcaaatcc gcccgctttc ttttaaatat tcccgcacca acaggcggtt
     1201 gatggcttgg atgtctgcag gctgaacagg aaattgctgt tccgttctaa agcgttttaa
     1261 tacgatgcct tgcatttgtt tttcaaagta attttggttg tccaaaatct gctccatatc
     1321 cagcactttt tggtcggcat cggtttttac accgcttagc gcgttaaaga ctttgagttt
     1381 gtctccgtag agggcggcgt tttccatcag ccgtttgtgg gtgcgggcgt atttggcatc
     1441 gccgccgtat ttgtgccgca agaggtcgtt ttgtcggttc agctctttga ttttggtata
     1501 gacggtttgg agtgtggcga tgtttgcctg catttcttcc tgcccgactt cggcgaggtt
     1561 ctttttcttg aagatgcgtt ccagctcttc gcgcaggctg atgtattcgg ggtcgtcttg
     1621 gtcaatattg cttgccagcc cttcgcggac tttgcgcatg atgtctttca aatcgtcggc
     1681 aagtttgagt tccgcctcgc caattttgac gaattgaaag taaacgtctt ccagcgcttc
     1741 attaagcaaa tgggcggtgt cgccttgctg tagtttttcg gcaaggttta aggtatccaa
     1801 acgggcggcg gtttcgcggt acaggagatt gagcttgtca aaatccagat gcgcgagaaa
     1861 ttcgtggcta ccttgcaggc gcaggatgtt gtacaactct ttggcggttt gcagggcttt
     1921 ttttagggcc agaagctgtt ttttgtcttc tatttggctg atttggctgc aaaattcttc
     1981 ggcgttttcg gtatcgaaat caaacagggc gtttttaatg tcagcaattt cctgttcgat
     2041 ttcttcggcg gttttgaata gctggctgta actgccgatt tcgtcgccca attcgttaga
     2101 aagttcgtcc caataggcgc ggttggtttt gtcaaattcg cgctcgatgt cggcaaaatc
     2161 gacgacatag ccgtaacggt aagatttgta ggtgcggttg acacgggtca gggtttgtaa
     2221 aaggttatgg gcttttatca gtctgcccaa atagagtttt ttcaggcgcg gcgcgtcaaa
     2281 gccggtcaaa agcatgttgt acacaaacaa gatgtcggtt ttgcccgctt tgaaatcttt
     2341 aacccattgg tcgcgttctt ccttagtgcc gacgtcgtgc aatatcaacg cggcggtgaa
     2401 attgtggtct gaggccgtct gaaaatgctc aaaaagctgg cgtgcctgtt ctgcgctgtc
     2461 gcaaacgacc atggcaccga ggctctcgtc ttggttggtt tttctgaatt tggcgaagtc
     2521 gtccaagatg tagtcaagca tcgggtggac gaagtgcgga tgggcgtaga tttctttgcg
     2581 gtcaaagctg cctttttcga tttcaagttg cgccagtgct tcttgtaatt gcgccttgta
     2641 tcggctgccg atttcttcgc gtatcaggcg cagggtgtag ccgtcggcaa tggaggcgtt
     2701 gtaatagtat ttgtggatgt agtcgccgaa gagttcgcgg gtgttgacgt tgcctgcggt
     2761 tacgccgata agcggcgtgc cggtcagccc gattttgacg gcgtttacgt cggactgatt
     2821 aaggttggca agaaatgagc ctttggggtt gtagctgcgg tgtacttcgt cgagaaaata
     2881 gacgcgctga atggcgaggt cgtagtcgtt acgggcgaca acacgggggc gtctttgaat
     2941 ttttggatgt tgacgacggt gatttccggt ttgcctgcgt ggttgtgcag ggtttgggcg
     3001 gatttgatgt cggcaacgaa ggcttcgcgg ctgtcgatgg tatggacgat taagtcgcgg
     3061 gcggtaaatt cccgctgcgc ctgtttcaat aaatcgagcc tgtccacgat gaaatagaat
     3121 ttcggcacga taccctgttt ggcgtaatag tgggtcaggt aacgggtgtt gtaataggca
     3181 ggcgcggttt tccccgatcc ttgggtatgc caaatcacgc cttttttacc gccgttcgat
     3241 aaatgttttt caatggcaag ggtggcaaac agttgcggat aacgcatgat gtgtttttgc
     3301 accgaacctt gacttgcttt gacgtaagtc agcccgtgtt gcaggagaaa ggcaagacgt
     3361 tcgcggcaaa gcagcgaagt caggatgcgg ttggtcggcg tatcgggcga tttattgctg
     3421 ataaattcgg ggctgtgttt gataacgggc agattattgt cttgtagaac gttgttttca
     3481 aggtcgtctg aaagcgtgtc caataattcg gtaaggttta atttatgctc ttcgcggaag
     3541 tagttgaata cgggcttgcc gcaagcagac gaggcgtaaa acgcgccttg tgccggctcg
     3601 gttgcgccgt cgtcgtattc catgttgttg gaaaaaatca tgaattgggt gatgttgata
     3661 aaacggcgga atttgggatt tttcgcacgt ttgcccatgc gctcccgctc ttcgccgatg
     3721 ccgcctttat tgttgggctt tttgacttcg ataaagacca aaggcatgcc gtttaccagc
     3781 agggcaatgt cggggcggaa tgcttcgtcg ccgttgatac aaggcaactc agttacaacg
     3841 tggaaactgt tgttgtcaaa attttggaaa tcgatcagct tcttaccgcc ggattgattg
     3901 gtgaggcgtt cgtaaaattt ttgccccaaa tcttcgttat ccagctctaa gcgtatgtcg
     3961 gcaagcaggc ggcgtgcatc atccggcggc aaatcgggat tgatgcggca gaggctgtct
     4021 acaaagattt cgggaaagat attggtttgt ctgtcccaat ttgcattttt tagcgaaaga
     4081 taatcatagc ccaagcgcat caggtgcaaa acggcgggga ttttgacgcg ggaattttcg
     4141 ttgtgggttt gggacataaa cgtaacgtaa tgggtcatca aaatttattg aaacggatta
     4201 taacacctga aaaaagccga tacgcctttt cggtgtaccg gctttgccat actgttctgc
     4261 ttcagacgac atcgcttcat tttgccttta atgcctcttc gtccagcgat ttcaaccatg
     4321 ccagcttttc gccgattttg atttccagcc cgcgcgggac gggttggtaa aagtccggtt
     4381 cgtccaagcc gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg
     4441 cgtagcggta ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcgttgcgca
     4501 ggtggacggg cacttcgtcg ctggcgtttt ctttgacgaa gcggcgcatt tggttgtatg
     4561 ccttgtagcc cgcgttggat ttggcggcgg cggcaaggta cagcacggct tgcgccagcg
     4621 cgagttcgcc ttcgggcgag cctaagcgtt caaaggtggc ggcggcatcg ttggcgattt
     4681 ggaaggcgcg cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg
     4741 cgagatagcg cgggtcggtg ccgccgtcga gcatacggca aaaccaatac agcgcggcgt
     4801 tcggatgcga accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc
     4861 cgcctttgtc gaaacggcgg atttgcgcgc ccaagctgtc ggcgagaaat tcggtggtca
     4921 ggattttcag acggcgcgtg tcggcggcac gtaaaagttg ttccaacaaa ttcaataatc
     4981 tgcgcgcatc accgtcggcg gtattgacga gcagctcttg cacatcggct tcaatcgtga
     5041 aatcccggta ttcgggcaaa gccaacactt tggcaatcag tttcttgagg tcgtctgaag
     5101 acaaggattg caaaacatat acctgcgcgc ggctcaacag cgcgggattg acttcaaacg
     5161 acggattttc cgtcgttgcg ccgataaagg tcagcaaacc gctttcgaca tgcggcaaaa
     5221 acgcgtcctg ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc
     5281 gtccctgctg caaagcgatt tcagccttat cgattgcccc gcggatgtcc ttcacgccgg
     5341 aaaacacggc ggaaacaggc aaaaactggg cattgaaact ctgcgccaaa atccgcgcca
     5401 acgttgtctt gcccacgccc ggcggccccc acagcaacat agaatgcggc ttcccgcctt
     5461 ccaccgccac gcgcagcggc ttgccttcgc cgattaaatg ctgctgcccg atgacatcat
     5521 caagcgtatg cgggcgcaga cgttcggcaa gcggtgcgtc gggttctcgg acaaacaaat
     5581 cggtcataac ggctccgtca acaggttttc agacaatatg attatacggc agggaaaggc
     5641 ggcgtgccgc atacggatac cgccccaccg tttgtactaa gccgatctta ggcgtacaat
     5701 tgggaaattt caggaaattt atacgctatg ccatccatgc agctttgcaa ccggacccgc
     5761 aaagaccagg tcccgtttta accatgcggg gaagttaaga ttacagtaaa caaaactatt
     5821 ttgccttatt ggaagaaagg ggaacagtta tgagaaattg gaacttatta ccaccgggga
     5881 aggggcagat ggagacttac tggtcaacgg ttcttacggc atccgttctt ttgcagacct
     5941 aaaattggaa tacaaacccg aattatcggt gagaagcaat catgacatta ttttcaacaa
     6001 tgattttcaa tctgttgacg tacaacccta ttccggaaat atccatttgg atgccaaagg
     6061 aaaaattcaa tttaatttga atgttgccga taaggtaaca gatccaacca cacatcaaga
     6121 aaagttagtg aacgataacg ctttagtgcg gtatgtcaac aatatcaccc cctccgatga
     6181 aaatgacaaa ggcagcatat ccttccattc aaaaacaggc aatttgttca atatgaacgt
     6241 taacgttcat aaaggcacac accatttcgg tattcacaac ctccgcaata ccaatgttaa
     6301 tctgactgcc gaagacggcg ataatgtggt tgcgattaaa gggaagcttg ccgagcctat
     6361 gccggcagga aagttgagag tacaagtttt tttaaggggt attgaaacgg cttttaaatc
     6421 caataccgta ttaacgggga ataacaataa aatatccatc accactgagg gcgacggctt
     6481 gggattgggc gtaaatggcc tccagtccaa ctccccctat tccggacaca cgacgggtac
     6541 tttcatcaat aatcaaccaa gcaatactac ccaaccttcc cttgacggca ccagcaccat
     6601 cgaactcaaa gccgtcaagg ggaataatga agttgattta aatgtaaaaa accacgcctc
     6661 cgtaaaaggc attatcacgt cgcattctgc aaaagcaacg cttgaagccg aggaggataa
     6721 tattgtccgg gttaaaaatc cggacaccaa gacggcttta atcaatgcct ggaaagaaaa
     6781 ataccccaat aattcgccag aaccatacag ggtggggggt tcaatccacg attgacagcg
     6841 atgtagtttt gagtgcggca aacaatatcg ttgaagtagg aggcagcccg atgaaccaag
     6901 aagggattac cgctcacgga aatgccacga ttaccctcaa ggcgaaagaa aacaataaaa
     6961 ttaccgtgga aaacgccgca tacagcagcg acggcatttc gactctgatt aacagaacgg
     7021 gggcaagacc cggaacaagg gatgatggaa ataaaatcat actggaagcc ggcggcgata
     7081 atattgttac catgaaatcc ggcgatgcgg atgcggatta tgtaaacaat tccaaagtat
     7141 taacggagac accatattat aaaagcaaac gaggttccaa cggcattttt gcctatggcg
     7201 acaaatcgct ggtcaaactg attggcgaga ataatatcgt taagagtgaa atcagtgaaa
     7261 aatctaaggc attaaatggg ggatttcgcc atatcggcat ttattcatgg caaaacgcga
     7321 aagtcgaatt gtctgcgaag agcgacaata tcgtacaagg cggaatttgg ggcttatact
     7381 ccaacaactc ctcaatttcc ctcaagggga aaaataatgt gatttcaaac ccgaaatata
     7441 atgttttcgc ctacaaaaag gcaaaggtgg atttgactgt cgaaaataaa aacacattat
     7501 ctgatgcgga atttggcgta tatgccttaa acacaagtat ggttaatttg tcttcaaaag
     7561 ataataacga ggtaaaaagc acccaagtgg gtctgtattc gcaagacggc ggttcaatca
     7621 atgtagatag gaaggataat attattgaag gcgacgcggt tgccttggtg ggaaaaggtg
     7681 gaagtcaaaa cattcgggca agccgtacaa acctgattag ttcaaaaagc ttaggaattc
     7741 atgctgaaca agctgcaaaa atagccataa ccggcgcaag caatacaatt catgcaagca
     7801 atgccgctat tcgttcatta gacaaaagcg aagttaagat tgacggtcaa attaccattg
     7861 actccaacgt tgccaatctt gcaaggcaag atggttcaat tcatttgaat tataaagacg
     7921 atacccgtat cacaggggca accgtatctg ataagggttt ggtagccatc aaacctttga
     7981 ataacacgaa tattgttgcc gacactattc actataaagg cgatgtcttg gcggtaaata
     8041 agggtaaagt ggaattagat ttcacgccga acatccgttt agtgggacgt ttggataatt
     8101 ttagcggctt aaccgattcc aaacataaaa atttattcga aaactatgtt gcaaatttag
     8161 acagcaaaag tgcgggcgaa attaacttta atttagccaa agacgcatta tggacgatga
     8221 caggtcaaag ctggctggat aaattggaag gacaaggcac tatcgatttt aataatgatg
     8281 ctaaaacaag tggacgcgcc ttacatatcg gcgaattggc gggtgccaat aaattcttga
     8341 tgcatctgaa taaagacggc attcacagcg atatgctcta tgtgaaaaaa ggcacttcga
     8401 caccgcaaga agtcgtcgtc aaaaatctgt ccgaagtgct cgacagtatg aattacggcg
     8461 aacgtttgcg tttcgctaca gtaacaaact caaaaaatga atttgtgaac ggtaaaaaat
     8521 atattgacga tacgcacctt atggaggatg ccctgactgt cgaatactcc gcacataacg
     8581 gcgataaaaa caacaaggat gactataata aatcctttaa cggctctgaa atgacggcgg
     8641 aaaaagctgg agacgattat gtcaataaaa cctataccga caacaggcaa aatgtctatt
     8701 tggtcaaaca ggctaccggc aatccgagcc gaaatgtcaa aaatatcaat gatatgttcg
     8761 attcaaccgc acattatgcg ttcactttgg atacttatgc caaacgcgaa ggggagcggg
     8821 ctttttcaac gttggataaa aaagaaggcg attggataag gctgacgcat acccgtgtga
     8881 ttcaatccaa tgcgtttagg tttcataaca acgattttga aatcggatat gaccgattca
     8941 gcctcaacga gcaggagaaa aaacgcaaat ggggcataag tctcgactac ggccacggca
     9001 ggacatcatt atggaatacg tttggcaagg acaaaatcag gaaatatgaa ttggctctgt
     9061 acaatactac ccaatacata gataaagaag gagacgaaac agggtatatc gacaatgtat
     9121 taaaaatagg aaaactgcgt aaccgtgtga ttgcacgaaa tcatatgggg caattatggg
     9181 gcaagggaaa atatagcaac accctattct ctatcagcac cgaatacggc cgccgtaaat
     9241 ttttggatga cgataaattg tggcggatta caccgcaagt acagttgcaa tattcctatt
     9301 tgagaggtac cggctatcgg atcgataacg gcataaacgt caatttaagc cacgcaaaca
     9361 gcctgatagg ccgcttgggt ttggatgtcg tgagaaaatt tgacggaggc aaaaaacttt
     9421 tctatatcaa aggcaatatc tttcatgaat ttttgggcag tcgttccttt aaggcatttg
     9481 agggcaaaag tcattatgct caaaaatgaa atacgagggg gacttggtat tccgccggat
     9541 tgggctacaa tgcgcggaca ggcaaaaaaa acgaatgtct ttgccgatgc ggaaaaaaga
     9601 gttttccggc gggaaaaaag gatcgtacaa catccgctta tccgtttccc atcagtttga
     9661 ctgaatgata tcggggccgc gccattattc ggcaggcccc attttattgc cacacattgc
     9721 ctgcccgatt cccgtttcaa cagcaaaccc ttccaatatt ttgttcaaac ttggatcgcg
     9781 gcagatgggg gcgtgcaagc cccgcaaggt ttttttggct tttacggaac agcacgcatt
     9841 tcgccaaacg gcaaaattgc attaacggaa ttgaaaactt ttggaattgt gcaaaacggc
     9901 atttgcgtca gtttaacggc atttccaaag ggcattttaa gccgtattcg agagaatgcg
     9961 gatggcgttt tcagtgtttt cagcgtgata aaattcaaat ttctattcta aaacaatcag
    10021 caaagcagga tttatttcag ttgtccgaga caacgggcgg ctgcaccgcc tgcactcccc
    10081 atactttttc aacgcactcg agcaggagtg agacgtgggg attatcctcg tattcttccg
    10141 cataaatgaa attgagcggc atactgtggc ggcacgattc gataatagcc acgcccgcgc
    10201 cttcttttgc cgccgcttcg gctttgtttc cctgcaccat tgccacacct ataccgcctg
    10261 caaccaaatc gataagggtt tgggggtagt cgcacaatat ctgttttttt gggtgagagc
    10321 cggttgctgc gccaaaactg gtgcaggtgc ttcctacttc cggacacgcc cgacatttct
    10381 atccatacgc attcttgcag gctctgcgga agggagcggg tcagccggga atattggctt
    10441 tgcgggcaaa tcagcgcgta ggtcaggttt tgcaggaata cgctgcggat gccgccttgg
    10501 gcggcgttgc cgagtataaa gccgccgtgc agggttttgt gttggatgcg cgagaggatt
    10561 tcgccgctca ttccgtattg gatgtgcagg cgcgttttgg ggggctgttt gaccgatatt
    10621 gtccgtcagc gcgacgagtt ttgccgaatc gatggggtgg ataatgccca aactggtctc
    10681 ttccgaataa tcgcctgcca gcgttttggc aaaatactcc aacttgtgtt tgtattgcag
    10741 cagggattcc gcttcgggca acagtatttc gcccgcccgc gtcaatacca tgcctttccc
    10801 cgtgctcctg aacagcggcg tgccgacata ttcttcaagg gctttaattt gggcggaaac
    10861 ggcgggctgg gaaaggaaaa gtcgtttggc ggtttgggta aggttgccct cgtgcgcgac
    10921 ggcgacaaat gattttaatt gtacggcatc catgtatccc tccttgtgcg gatgttttct
    10981 atatttgtgc aatcgaaatc ttttaggcgg attgttgctg aaaattaact ttttaatcaa
    11041 gtggtttgta aattgtatca gttttccgga tgatgattat taaaaaaaaa ttggttttat
    11101 tgcctgtttg ggctttaaat ggggttacgg cttccgaacg cagcccgtat caaaaagaaa
    11161 agtcatgcgc ccccttttac gaggcgcgat atataaggag gaaggttatg gaaaaacata
    11221 atgggactta tcgggattta caccgtccgg cttcggaatt tgcgacgcgg gacgaatatt
    11281 tggaacatga attgcagatt atgcaaccaa aacgctggcg gcccaacctg ccctttcgcg
    11341 attaccgctt cgagtgggag gatttgattc ctgcgatggc gggaacgatt ggaaaagtgg
    11401 tgatggtggg ggggcggcgg cgtttgccgc acctttgggg ctgcctgaca gctttgtact
    11461 ggaaaatgtg cgctatgagc ttttaatcgc cgccgcgttt atcttattgg tatcgggctt
    11521 ttttctgccc ggcgccaacc tgcccggtac gcacgggccg ctgattccga tgattcccat
    11581 cgttgtgtcg gcaggcgggc atcctttggc gttcggcatt tcgattgcgg ttttaggtct
    11641 gctgatggct ttatttcgcg gcggcagtat tatggcgaag ctgacaagca acggcgtatg
    11701 cggcggatta ttactctatt tgggctttat cggcacgacg gggcaggtaa aaaaattgtt
    11761 ttcgtgggca ggcggtttta atatgcccta catcgctttt accgtcatta ttgtaacgat
    11821 tgtgatgtac gctttgttgg agcattggaa aaaacgctgg ttggccgtgc ctttgggatg
    11881 cttgattgcc ggtgtggcgg catttgcatt gggtgcgccg tttgagtttc acaccgcccc
    11941 cggcctgcct ccaatgagtc ctgcttattg gtagggtgaa aacagcggct ggcatctggg
    12001 gttgccgacg gcagaaagtt ttttggttgt ctttccattt gcggtattgg ctgttgcaat
    12061 gtggtcgccc gattttttag gacatcaagt gttccaaaaa atcagctatc cggaaaaaac
    12121 cgataaggta ttgatgaata tagacgacac catgacaagt tgttctgtcc gtcaagcagt
    12181 gggttctatt ttagggggtg caaattttac ctcttcttgg ggaacttata tcgtgccggc
    12241 atcgattgcc aaacgcccca ttccgggcgg tgcggtttta acggcggttt tatgtattat
    12301 cgccgggttg tggggctatc cgatggactt ggcgatttgg cagccggtat tgagcgtagc
    12361 cttgatcgta ggcgtatgct taccgctttt ggaagcgggc atggaaatga cgcgcaaagg
    12421 caaaaccacc caatccgccg ccatcgtggt gttctcttcc gtctggtcaa tccggttttc
    12481 ggctgggcgt tgacgatgct gttggataat ttgggcttaa tcggctgcaa agaacgcagc
    12541 gcgcaattag gttttgtcgg acgagtattg atacccgcag taggtttctt aatcttgtgt
    12601 gtggcgatgg gtgcggtcgg gatgctgccc ggtatccctc cgtttttgga gcagttcaaa
    12661 tctttgggct aggctgaaat cgcaaatgcc gtctgaaccg gtttcagacg gcatttttgc
    12721 aaacaggcaa aatgacggcg gcgggatttt ttattttccc gattgaagta taatgttgcc
    12781 gggcttcaac cggatattca aacggtttgc tccaacactc ggaacggcgc ataaaacgcc
    12841 gcccttcgcg ttatcccgaa cggggcggct aatcagatcc tatcgccata aaaggcgggg
    12901 tttcaaccga aaaggaattg agatgaataa aaccttgtct attttgccgg cggcaatctt
    12961 actcggcggg tgcgccgccg gcggcaacac attcggcagc ttagacggcg gcacgggtat
    13021 gggtggcaga atcgtcaaaa tgacggtaga aagccaatgc cgtgcggaat tggacaggcg
    13081 cagcgaatgg cgtttgaccg cgctggcgat gagtgccgaa aaacaggcgg aatgggaaaa
    13141 caagatttgc ggctgcgcta ccgaagaagc acctaaccag ctgaccggca acgatgtgat
    13201 gcagatgctg aaccagtcca cgcgcaatca ggcacttgcc gccctgaccg tcaaaacggt
    13261 ttccgcctgc ttcaaacgcc tgtaccgcta accttcttca gacggcatga ggcagatgcc
    13321 gtctgaatcc gttttcctgc aatttcgaca aacgagaaaa atcatgagat acatcagcac
    13381 gcgcggcgaa accgcacaca aaccgttcag cgaagtttta ttgatggggc ttgcacccga
    13441 cggcggactg atgctgccgg aacattatcc gcaaatcggg cgcgaaacct tggacaaatg
    13501 gcgcggtttg gcttatcccg aattggcgtt tgaaatcatg tgcctgttcg ttaccgatat
    13561 tccggaggac gatttgcgcg acattctgaa ccgtacttac acggaagcgg cgttcggtac
    13621 taaggaaatc acccccgtcc gcacgctttc agacggcatc aaaatccaag ccttgtccaa
    13681 cggcccgacg ctggcgttca aagatatggc gatgcagttt ttgggcaatg cgtttgaata
    13741 tgttttaaac aaagaaggca aaaaactcaa tatcttgggc gcaaccagcg gcgatacggg
    13801 ttcggctgcg gaatatgcct tgcgcggcaa aaaaggcgtg aacgtattta tgttgtcgcc
    13861 cgacggtaaa atgagcgcgt tccaacgcgc gcagatgtac agcctgcaag acgggaatat
    13921 ccacaatatc gccgtgaagg ggatgtttga cgactgtcag gacattgtga aggcagtgca
    13981 gaacgatgcc gcgttcaagg aaaaatacca tatcggtacg gtcaattcga tcaactgggg
    14041 gcgcatcgtc gcgcaagtgg tttattactt tgcaggctat ttcaatgcca cttcaagcaa
    14101 tgacgaaacc gtcagctttt gcgtaccgag cggcaacttc ggcaacgttt gcgcgggaca
    14161 catcgccaaa caaatgggcc tgcctgtccg ctgcctgatt gtcgcgacca atgaaaacga
    14221 tgtgctggac gagtttttca aaaccggcgc ataccgcccg cgcaacagcg cgcatactta
    14281 tgtaacctcc agcccgtcta tggacatttc caaagcgtcc aacttcgagc gtttcgtgtt
    14341 cgacctgatg gatcgcgatc ctcaggaaat caatacgctg tgggcggaag tcgcggcagg
    14401 caaaggcttt gacctgcggt ttgccttgga caaagtcggc ggcaaatacg gctttacctc
    14461 cggcaaatct acccacgccg accgcctcgc caccatcaga caggtttacg agcaagacaa
    14521 agaactcatc gacccgcata ctgccaacgg cgtaaaagtc gcccgcgaag tgcgcgaagc
    14581 gggggaaacg gtggtttgtt tggaaaccgc gttggcggcg aaattcgatg cgaccatacg
    14641 cgaagccgtc ggcgatgtcg ccattccgcg ccccgccgcg ctggaaggtt tggaaaacct
    14701 gccgcagcgc gtgcaaaccg tgccgaacag tgcggatgcg gtaaaaggca tcatcgaaca
    14761 aacccttgcc tgatgttccg tatgggcggc attccatccg aacttaagat tgaaagaaaa
    14821 tgaccttacc tatgcaggaa acccgttttt ctatactgct ggacgaattg gcggcaaaac
    14881 aggaggcaac catcgccccc tacctgcttg ccgacgggac caaggtatgg gtacgcaaag
    14941 caggcaggca caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga
    15001 aactgggggt gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat
    15061 caaaacgcct gtacgaattg cgttcggcag ggatagccgt tcccgaattg ctcgccctcc
    15121 ggaaaaatgc ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc
    15181 aagaagccga agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg
    15241 tgcataaaaa acggcagttc ctcagtcagg cgtttgcacg aaatatgatg tgggacggaa
    15301 aaaacatcag ctttttggat tttgaagacg acccttccga ggtcctgacc atagcgcaat
    15361 gccaagcccg cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg
    15421 gactgctgga agcggctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa
    15481 tacaaaagct gattgccggc accgtcaaac cgattctccc gatacgcagg ctggaacacc
    15541 cccgctgggg acgggacgcg ctcaggctgg cagcctcgat ttccttaatt tccctcgccg
    15601 atatgccgcc gtaagccacc gcccgcccca cgatgccgtc cgaagccctt cagacggcat
    15661 ataaccaacg cgcatcaggc attgtcgcaa ccatccgttt cgtttagaat tttgacttta
    15721 tcccgaatcg ggcagacatt tccactctgc cgcacgtccc gaacaagcac tatccatttc
    15781 ttttactgac aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac
    15841 cactggacag acgcatattt tacctttacc tgcatccgcg acgaatcgtt gcgctttgaa
    15901 aacggacagt tcgtcatggt cgggctgatg gcggacggca agccgctgat gcgcgcatac
    15961 agcgtcgcct ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac
    16021 ggcccgctga ccagccgcct gcaacacctc aaagtcggcg acgaagtgtt aatcagcaaa
    16081 aaaccgaccg gaactctagt tgcctgcgat ctgaatccgg gcaaacacct ttacctgttg
    16141 agtaccggta ccggcatcgc ccctttcttg agcatcacca aagaccccga aatttacgaa
    16201 cagtttgaaa aaatcatcct tgtacacggc gtgcgctaca aaaaggattt ggcatactac
    16261 gaccgcttta ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa
    16321 ctgatttact acccgattgt ttcccgcgaa gaattcgaac accgcggccg cctgaccgac
    16381 ctgatggtaa gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa cccccaagac
    16441 gaccgtgcca tgctgtgcgg cagccctgcg atgctgaaag acacctgcaa agttttggac
    16501 gatttcggtc tgacagtctc cccgaaaacc ggcgtgcgcg gcgactacct gattgagcgc
    16561 gcatttgtgg atcaataagc cgcttgaaat gccgtctgaa cggcatcagc aatcaaaaac
    16621 cccgaatctt gccaatcagg attcggggtt tttatagtgg gtaaaaatta gaaatgcaca
    16681 tttccgtcat ttccgcgcag gcggaaatcc agacctatcc gttttattag tgtttggaaa
    16741 taaaagagaa accaagcata tggattccta cctacgcggg aatgacgacg gtgttttgcc
    16801 gtagccaaag cagaaaaaca tccctattgt cattttatca tgataggaca gcaaggatgt
    16861 taaaaattaa ttatcattat gtgattgata tataagaaaa attgtcacaa taagaaaagt
    16921 tatgcagaaa ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccaccgtc
    16981 atacacagag ccagtatttg gaaaaaaata atccatattg acatcattat tccatttatc
    17041 gcataaatcc actttttaat ttttttcctc attaatataa tctcttattt aataaaatca
    17101 aatacctagc aattcgatct caaattttaa acgccaacgt gtttgagcgg taacaatgca
    17161 tactttaagt ttgtaatcct tcatcgtctc atttttgttt taatcgacta tacgtctaaa
    17221 gcaacgccgc ccgtcattgt tcggacagta ttttttcaga cggcattttc ccccgtttgt
    17281 gaaggcgcaa ggcattgccc aaaaccgcca gcgaactgaa actcataccc agtgcggcta
    17341 tccacggttg gacatagccc aaaacggcaa gcggtacggc gatgatgttg tacgcgcccg
    17401 cccatatcag gttttgccgg ataatatggc gggtgcgccg cgcctgatcg agcaggtggg
    17461 cgacggtacg caaatcttcg ttcaataaca caatgtccgc gccgtccctc gcaatatccg
    17521 tcccgcccgc tgcggcggcg gatacgtctg cctgcgccaa aacgggcgcg tcgttgatgc
    17581 cgtcgcctat catcagcact tttttccctt ctttttgcaa ggctttgacg tattccagtt
    17641 tgtcctcggg catggcttgg gcgcggtagt gcgcgacacc cagggcgcgt gcggtttcgg
    17701 caacggcggt ttcgcggtcg ccgctgagga tgtgcagggt caggtttttg cctgccaact
    17761 gccgcactgc ctccgccgcg ctgcctttga gcgggtcttg caggtagaac acggcttgga
    17821 aaccgctttg actgccgagg taaaccgcgc tgccgccgcc ttctgtttgc ggttctttac
    17881 ctgaaatttc ggcaacatag gatgccctcc ccaatgccca aacctgtgtt tcgccgttga
    17941 cggtcagttg cgcacccacg ccttccccga tgcggttgag gcgttgtccg acttggattt
    18001 cggggacgct gccgcctgaa atgcggcagt tgagaatggc gcgcgcgagc gggtgttcgg
    18061 actgctgttc caaactttgc gccactgcca gcacttgggc ttcggtcatg ctgccgagca
    18121 gttcaatccg gcggacagcg ggattgcctt gggtcagcgt gccggttttg tcgaaaatga
    18181 tgtcggtggt ttgggagagg gtttcgatta cctgctttcc gccgattaaa ataccttcgc
    18241 gcgccagcgt accggtagaa gctgccagcg cggtcggcgt ggcaagcgac agggcgcagg
    18301 ggcaggtaat gaccagcagg gcgacggtaa tccacaatgc ggtgtgcgcg tcggcgtaca
    18361 gcgtccaacc gatgaagacc gggacggcaa gcaggagctc gccgaatatg aaagatgagg
    18421 cgtattgttc cgccaactcg gcagtgcgcg gtttttgcgc caaggcgcgg tcgagcaggc
    18481 ggacgatgtg cgacagtcgc gtgccgccgc cggtgcggtc ggtgcgtata atcagggggc
    18541 tttgcgtgtt gagtgtacct gcggttactt tttcagacgg cattttggcg acgggcaggc
    18601 tctcgccggt cagcatagat tcgttgacgg cactgtttcc ttccagcacc gtgccgtcaa
    18661 cggggatggt ttcgccgggt ttgaccatca caatatcgcc tgcttggagt ttgacgacgg
    18721 cggattcgcg gacatcctgt acggcaggat agccgggcat ccggtggcag aaggcgggta
    18781 tcagtttcac cagcctttcg gcggcatcgc ctgccttgcg tcgggcaatg tgttccataa
    18841 agcgtccgcc cagcaggaaa aacagcagca tcgcgatgga ttcgaaatac atcccctgcc
    18901 ctgcgttggt ggcgaggctg taaatgccgg cgatgaaggt catgatgatg gcggcggcaa
    18961 tcggcgtgtc cataccgacc cggcggtttt tcaggtcgtg caacgcgcct tgataaaacg
    19021 ggacggcgca ataaaatacg acgggcagca ccatcaggaa gccgccccaa tggaggatct
    19081 gcaggaaatc gggttcgatg tcgccgccgt aaaggtaggt cggcagcgcg aacatcatcg
    19141 tctgcatcat acccagtccg gcaacggcaa ggcggacgat gtattgtttg cgttcttttt
    19201 ggttggcggc ttcgattttt tgcgcgtcat agggtgcggc ggtgtagcct gtctgccgga
    19261 ttttcaacag aatgtcggag aggcggattt tgccgtcgtc ccagacgacg cggcagcggt
    19321 gcgtgctgta attgaggtcg atgcggacga tgccgtctgt acgcaaaagc tgctgttcga
    19381 tcagccagac gcaggcggcg caggtaatgc cgctgagcat cagcactgct tcgtgcgtgc
    19441 cattatgggt ttccacaaag tcggattgga cttcgggcag gtcgtacagg cggatttggt
    19501 cgaggatttc ttggggcggc agttcggttt ttttcgcgtc ggcggtgcgt cgtttgtaat
    19561 aactgcccaa gccggaatcg atgatgcttt gtgcgactgc ctgacagccg acgcagcagg
    19621 tttcgcggtc ttcgccttcg tagcggacgg tcagatgcag gttttcggga acgtccagcc
    19681 cgcagtggaa acaggttttt ttcatggcat ttcggtttcg tctgtgtttg gtgcggcggc
    19741 acaatactcg gcaatggctt cgcgcagtgc gtctataccg gtattttcag caacggaaat
    19801 gcggacggcg gcaatttttc ccgcagcgtc gcgccatatg cccgtgtttt gttcttcaga
    19861 cggcagcagg tcggttttgt tgtacacctt gatgcacgga atatcgtggg catggatttc
    19921 ttgcagtacg ttttccacgt cttcaatctg ctgcccgctg ttcggggcag cggcatcgac
    19981 gacgtgcagc agcacatcgg cttgcgcggt ttcttccagc gtggcggaaa atgccgaaat
    20041 cagtttgtgc ggcagatcgc tgacgaatcc gacggtatcg gtcaggataa tgctgcatgc
    20101 gggactgatg tacagccgcc gcgccgtcgt gtcgagagtg gcgaaaagct ggtctttcgc
    20161 atatatgccc gacttggtca gccggttgaa caggctggat ttgccgacat tggtatagcc
    20221 gaccagcgca aacgttttga ttctgcccga ctcgcgggac ttgcggcgca gggcgcgctg
    20281 ttttttgagg ttggcaagct gttttttcaa ggcgttgatc cgatgggcgg ttaatcggcg
    20341 gtcggtttcc agtttggttt cgcccggccc tttcatgccg ataccgccgc gctggctttg
    20401 caaatgtccg taaccgcgta tcaagcgtcc cgccaaatgg ctcaattgcg ccaactcgac
    20461 ttgcagcctg ccttcctgcg tgcgggcgcg gcgggcgaaa atcgccagaa tcagccccac
    20521 tctgtccaat acgcggcatt ggaggatttt ttccaaattg cgttcctgcg tgggagtaag
    20581 ttcgtggttg aatacgacca aatcaatgcc gtctgcggca actgcttccg acagctccgc
    20641 cgccttgccc gtgccgacaa acagcgcagt gtgcgggcgg tcgcgtttgg cagtctccac
    20701 gcgtacggaa tcgccgcccg ccgccttgac cagctcgacg gcttccgcca atgccgtctg
    20761 aaaaccgttc agacgggcgg cattgctgcc cgtatcatct ttatccaaca ttacgcccac
    20821 cagcatgacg cgttccggtt gcgcctgagt ggcactgttc cgtcctgttc tacctgacat
    20881 aactgtcaat cctgcaaata aagggttaca ccgcgttccg ggctgtcttc cggagtaagc
    20941 ctgatgcggc ttcccgccat tccgctgacg ctgaagacaa tgccgcccgc ttcgtgccgg
    21001 agtatggaca ggctgttgcc gaactgccgc cggtaggtat aacgggtaca cgcttccaga
    21061 caccagcgtc cgcgccagtg gatgaaatat ttttcgcctt ttctaaccgc aagtgtctcg
    21121 ccgccgttta cctcaaaata agggtaatcg ggttttcggg ttctaaactg ccatttcgcc
    21181 tgcgcgcgcc gtccgttgcg gacatagtcg aataccgccg tataaagcgt gccgtattcc
    21241 aaaggtttga gcgggaaaag ggcgaattgg tgcgcggtca gcctgccgtt agggtcgttg
    21301 ccggcggtta aaaccctgac ggggcggatt tcgtttttac cctgatacag cttgaaactt
    21361 ttcatcgcaa ttttgcctgc cgcctcggaa aaatcaatgc tggcaggatt gcctgtgatt
    21421 tcatattccg gcacggggtc gggacgttcc ccgtaaaaat aaggcagcgc gccgccgcca
    21481 accggatagg cggtataaag caattccgtt acgggcatgg cttcgtcagc ataaacggcc
    21541 gcaccgttgt ggcaagcgtt gcggtaatat ttccgtcctg cttccggctg ccgccttcct
    21601 tttgcacagg cgcgctcgaa gctgccgttg ccctgattga atacgaggac ggttttgccg
    21661 ttttcgcgca caaatgccgc acctgcttcg tcggtatggc ggtcaagcag cgaaaggcgg
    21721 tgatagacag cactcatcaa agcgtccact tggcgttgct gcgtgcggat gtcgctgtcg
    21781 gacgattcgg cggcttcctc ttccgtgctg atgttttcat gcacgccgtt gtagagatac
    21841 ccggcaaggc gtgtgcgttc ggtcagcttt tgtgcggtgt agtgcggatt gtcgggatgg
    21901 tgttccccgt gtccgtcttc gggattgagc gtgagatagc gtgcgtgcct gcgggcggaa
    21961 ttttccaaaa ccggcgcgtg tgccagcgcg tgcaaaccga tttgtgtgcg gatgcggttg
    22021 aggtagtgca gcgcatcgcc gccgtccctg atttgcggtg cggacggata gacaagttcg
    22081 cccgcgggca gggattggtt ttgggtatgg tagaaaacgc ctgccgccga gcccaataga
    22141 agcagccaaa taaaaaggga tttcataatg ccgccctccc gacgcggcaa cggggtgttt
    22201 tatcaaaacg gcaaacttgc caatgcggtt tccgcttgcc gattgccaaa ccggcaaacc
    22261 tttcccgtgc cggtgccgaa cccgatgccg tctgaacctt cagacggcat cgggtgttta
    22321 tttgtccact cggacgggtg caggttcgta ttgtgtcgat tcgtcgccgt aatacagcac
    22381 gccgagtttg acggggatgc gtccctgcga tttgcggtgg gcgttggaat cgcgcaagga
    22441 ataggcgcag ccgcagtatt cctgctggta gaagttttcg cgtttgctga tttcaatcat
    22501 acgcgcgccg ccgccgcctt tgcgccagtt gaagtcccaa taggccacat catcgtaagg
    22561 cgcggcggca cggtgtccgc agtcgttgat ttgcgccata tttttccagc gtgaaatgcc
    22621 cagcgaactg gtaaagacgg gaaaaccgtg ttcgtgcgcg tattgcgccg ctttttcaaa
    22681 acgcatatcg aaacacatgg tgcagcggat gccgcgttcc ggctcaaact ccatgccttt
    22741 ggctttggca aaccattctt tgcggtcgtt ttcgtagtcg tcgtctttat cgatgaaagg
    22801 gatgccgaac ttttccgcaa agcgcatgtt ttcctctttt cggagcatat actctttgtg
    22861 cggatggata ttgggattgt aaaaataaat ggtgtagtcg atgccggagg caagcatggc
    22921 ttccatcact tcgccgctgc acggggcgca gcaggaatgc agcaagactt ttttatgtcc
    22981 gccgggcggg acgagtatgg ggcggtcaat gtcggtaacg gtaggtttgt tttgtgtttc
    23041 cattaactta acaatatctg tcgaatacgg tcaaagcggc tattttacca gagccgccga
    23101 atgtaaaaca gggtgtcagg gcatcagccc gagctgcctg aacgaacgga cggtttgcga
    23161 ggtaacgata aaatggtcga gcagcgaaac atcaaccagc gacatggcct gtgccagccg
    23221 ccttgtgaac atgatgtctt cctgcgaagg ttcgggcgag ccgcccggat ggttgtgcgc
    23281 gataatcagg ctgtcggcat attcgtccaa tgccagtttg acgatttcgc ggatgtaaat
    23341 cgtgttttcc gcaaccgtac cgcgcgacag ctctctgacc gcaatcagtt ggttttggcg
    23401 gttcagcagc agcgcgacgc tgacttcgac tttttcctgc cccaaatgaa agcgtaaata
    23461 atcggcaacg gtatcgggat cggataaggt aatttcttcc tgcaattctt cctccaatat
    23521 ccgccgcccg atttccctga ccacggcgaa ctgtgtgaaa cttgccgtcc ccatcccttt
    23581 gtatgccgac agtttgccga cctccgcgct catcagcctc cccaagctgc cgaactcctg
    23641 caataggtag cgcgccaaat cgaccgcgct catcccgcgc gtcccgacgc gtaaaaggat
    23701 tgccaaaagt tcggcatcgc tcaaagccgc cgccccgcgt tccaacagct tttccctggg
    23761 cctttcgcct tccggccatt gcttgatgct catgttttcc cttttttaaa tagtctgaga
    23821 tgccgtctga aacagatgtt cccacacgcc aaatatccga aatgccgtct gaaccggatt
    23881 tccgcttcag acggcatctg tatttcaaaa cagtcttacg ccgtttcttc caactcgatt
    23941 gaagccgcca aaagcgacag acgtgccatt acgccgtaaa cgtagaggcg gttatgttcg
    24001 ttgtccacgt cgcagtcttt ttcagaacgc ggcataccga gcgaatccca ttgttcgaat
    24061 acgcgtttgc aggcttctgg agcttcttgc gaattatcgc cgttaccggt aggaatgctg
    24121 ttggacagcg gcacaaacac cataccgccg gcgttgaggt tttcgtccgc accgcgccct
    24181 tcgtgtacgc ggaaaaagcc gccgatgacg aaacggtcca tcatatagac gacgggttcg
    24241 cacaccgcgc cgttcaaggt ttcataggta taaacccctt cttggacaat cacttcgctg
    24301 acttccaagc cctctttcac cttcgccatt ttgttgcggt ttttgcggtt caagccgcgc
    24361 acttcgtcgg aggatttgac gctcatcaca cccatgccgt aagtgccggc atcggctttg
    24421 acgatgacga aaggtttgtc ggcaatgccc gattcgtcgt atttggcttg aattttcgcc
    24481 agcacgcgtt ctaccgcttc cgccagcgca tcttcacctt cgcgcccttg gaagtccaaa
    24541 ccgccgattt tttcaaaata cgggttgatc tgccattcat cgatgtcaat cagcttggca
    24601 aattcggcgg caacttggtt gtacgcgccg aaatgatttg ttttgcggcg cgtcgtccaa
    24661 ccgccgtgca gcggcggcaa aacggtttgg ccgatgcctt tgagaatgtc gggaatgccg
    24721 gcggacaaat cgttgttcaa caaaaccacg caaggcgaaa agccgtcggc aaggtggacg
    24781 cggtcgcggg ttcgcagcaa aggctcaagc aggattttgt cgcccaatgc agtttcaaat
    24841 tcggccggtt cggttacttc cggattcagg ctgcccaagc gcacttcaca ccctgccgaa
    24901 cggaaaatct cgccgagggc gtaaacgttt tgcaggtaaa acgtattgcg cgtgtggttt
    24961 tcgggaataa tcaatacgga tttctccgtt tcgcaggcgc gttgcaccgc atcttgcgcg
    25021 gcaaccgccg ccagcgggat gaaattcgga ttcagattat tgaagccgcc cggaaacaaa
    25081 ttcatatcga tagacgaaat tttgtaaccg gcgttgcgta tatcgaccga accgtaaaac
    25141 ggcgggcggt gcgcgttcca ctgcgtgcgg aaccacgcct cgattttggc gtgattggac
    25201 aggatttttg cctcaaacgc ctgaagttgc gccgaatgtt cgggcgacat aaccggtaat
    25261 ttcatctttc gtctgcctct gtgtgttttt tgacaaggta cagataataa tgcttttgaa
    25321 agaatgacga caagtattct ttcgtatcga atcattcttt agatataacg aaatgaacaa
    25381 tatgtctaat tttttcgtta aaattgttta caaaataaca ccgactcaaa aattagacaa
    25441 aatctgttgc gcagtataaa gaatacgtct aaaatccgcc caatccgcat caccatttat
    25501 ccaaaagaac aacatcatga cggcacaaac cctctacgac aaactctgga acagccacgt
    25561 cgtccgcgaa gaagaagacg gcaccgtcct gctttacatc gaccgccatc tggttcacga
    25621 agtaaccagc ccgcaggcgt ttgaaggtct gaaaatggcg gggcgcaagc tgtggcgcat
    25681 cgacagcgtg gtctccaccg ccgaccacaa caccccgacc ggcgattggg acaaaggcat
    25741 ccaagacccg atttccaagc tgcaagtcga tacgttggat caaaacatca aagagtttgg
    25801 cgcactcgcc tacttcccgt ttatggacaa aggccagggc atcgtacacg ttatggggcc
    25861 cgaacaaggc gcgaccctgc ccgggatgac cgtcgtctgc ggcgattcgc acacttccac
    25921 ccacggcgca ttcggcgcac tggcacacgg catcggcact tccgaagtcg aacacacgat
    25981 ggcgacccag tgcattaccg cgaaaaaatc caaatctatg ctgattgccg ttgacggcaa
    26041 attaaaagcg ggcgttaccg ccaaagacgt ggcgctctac atcatcgggc aaatcggcac
    26101 ggcgggtggc acgggttacg ccgtcgagtt cggcggcgaa gccatccgca gcctttcgat
    26161 ggaaggcagg atgaccctgt gcaatatggc gattgaggca ggcgcgcgtt ccggtatggt
    26221 tgccgtcgat caaaccacca tcgactatgt caaaggcaag cccttcgcac ccgaaggcga
    26281 agcttgggac aaagccgtcg agtactggcg cacgctggtg tctgacgaag gcgcggtttt
    26341 tgataaagaa taccgtttca acgccgaaga catcgaaccg caagttactt ggggcacgtc
    26401 gcccgaaatg gttttaaaca tcggcggcaa agtgccgaat cccgccgaag aaaccgatcc
    26461 ggtcaaacgc agcggcatag agcgcgcact cgaatatatg ggtttgaagg ccggtacgcc
    26521 gctgaacgaa atccctgtcg atatcgtctt tatcggctct tgcaccaaca gccgcatcga
    26581 agacttgcgc gaagccgctg ctatcgccaa aggccataaa aaagccggta acgtacagcg
    26641 cgtgttaatc gtccccggct ccggtttggt taaagaacaa gccgaaaaag aaggcttgga
    26701 caaaattttc atcgaagccg gttttgaatg gcgcgaaccg ggctgttcga tgtgccttgc
    26761 gatgaatgcc gaccgcctcg cgccgaggca acgctgcgcc tccacgtcca accgcaattt
    26821 tgaaggccgc caaggcaacg gcgggcgcac ccacctcgtc agccccgcta tggccgtcgc
    26881 cgccgccgtt accggacact ttaccgacat ccgcacgatg gcgtaagtat tttcaggcgg
    26941 tctttaagaa tttgacgagg ccgtctgaaa tatgtcccgt aaggcagaaa cacctaatat
    27001 tcaaaccaag gagtttaacc atgaacaaac ttttcgttac cgccctgtcc gccctcgcct
    27061 tgtccgcctg cgccggcact tggcagggcg cgaaacaaga caccgcccgc aaccttgaca
    27121 aaacacaggc cgccgccgaa cgcgccgccg aacaaacagg caacgccgtc gaaaaaggtt
    27181 gggacaaaac caaagaagcc gtcaaaaaag gcggcaatgc cgtcggacgc ggcatttccc
    27241 atctcggcaa aaaaatcgaa aacgccaccg aataaccgat ttgcgatgcc gtctgaaacc
    27301 gtttcagacg gcatcgtcag cacaagacgc aaacccatga aagcctttac caaaatcacc
    27361 gccatcgtcg ccccgctcga ccgcagcaac gtcgataccg atgccatcat ccccaaacaa
    27421 tttctgaaat ccatcaaacg cagcggcttc ggccccaacg cctttgacga atggcgttac
    27481 ctcgaccacg gcgaaccggg catggacaac ggcaaacgcc cgttgaaccc cgatttttcc
    27541 ctgaaccagc cgcgttacca aggcgcgcaa atcctgttga cgcgtaaaaa cttcggttgc
    27601 ggctcttcac gcgaacatgc cccttgggca ttggacgact acggcttccg cgccattatc
    27661 gcccccagct tcgccgacat cttctttaac aactgctaca aaaacggcct tttgcccatc
    27721 gtgttgaccg aagaacaagt cgaccggctt ttcaaagaag tcgaagccaa cgaaggctat
    27781 cggctctcca tcgaccttgc cgagcaaacc ctgaccaccc cgggcggcga aacattcaca
    27841 ttcgacatta ccgaacaccg caaacactgc ctcttaaacg gcttggacga aatcggactg
    27901 accctgcaac acgccgacaa aattaaagcc tttgaagaaa aacgccgcca aagccagcct
    27961 tggctgttta acggttaaaa cctatgccgt ctgaaggctg ttcagacggc attcctgata
    28021 acatataatt gctgcggcta atgtaaacag aggggcgata aattgttttc atcttttaag
    28081 cgggcttagc aacactctaa attaatgcaa ctctccattt caaataaatt tttagatttt
    28141 taatatgcaa caaattaaat ttattgactt attttccgga atgagtggca tcaggaaagg
    28201 attcgaacaa gcctgtcgga aacagtcggt tgcttgcgaa tgtgttttta cttcagaaat
    28261 caaaccggca gctttggaag tattgaagca aaactaccct gatgaagtgc cgtatggaga
    28321 tataacgaaa attgaaacag gggatattcc cgattttgac atcctgttgg caggcttccc
    28381 ttgtcaggct ttttctttcg ccggaaaaag attaggcttt gaagatacac ggggaacgct
    28441 tttctttgat gtggcaagga ttttaaaggc aaaaaaacca aaaggtttta ttttggaaaa
    28501 tgtggaagga ttggtgacac acgatagaaa agattcgaca caaaaaatag ggcgcaccct
    28561 gaccgttatt ttggaaacct tggaagcatt gggctactat gtttcttgga aagttttaaa
    28621 tgcaaaagat ttcggcattc cccaaaacag gaagcgcatt tatctgacag gcagtttgaa
    28681 atccaaaccc gacttatctt tcgaaacaag cccaagtccg aaattaaaaa atattttgga
    28741 atcgggactg cctactgaaa gcagcccttt catcaaaaaa ttgctaaaaa aatttccccc
    28801 gtccgaactg tacggaaaat cagtaaaaga caaacgggga gggaaaaaca atattcacag
    28861 ttgggatatt gaattaaaag gcgcagtaac cgaagaagag aagcaattgt taaatatcct
    28921 tctaaaagaa cggaggaaaa aaaaatgggc ttcagaaatc ggcatagatt ggatggatgg
    28981 gatgcctttg acaaaagcgc aaatttcaac tttctataaa caccccgatc tccaaaatat
    29041 tttggacagc ctgacagaca aaggctattt ggttttagag catccgaaac aaaaaattgg
    29101 cgggcaaaga atcaaagatg aatccctgcc caaaggctac aatattgttt caggtaaaaa
    29161 atcttttgaa atcaataaaa tattagatcc aaacgatgtt gcgccaacct tggttgcaat
    29221 ggatatggag caccttttcg tcgttgacaa cggcggtttg cgtacactta ccggaaaaga
    29281 agggttacgc ttattcggct atccggacga ttattcgttt gatattccca aaaaagacag
    29341 atgcgattta ttgggtaata ccgttgccgt ccctgtgatt aaggcggtat ctgaaagact
    29401 tctgcatact ttataaaccg acagggataa aatgataaaa cttactgcac aacaaatatt
    29461 tgataagttg ctggatgaag aaaaaatctt atcggccaat ggtcaaatta gatttttctt
    29521 aggagatgtg gatattatcg tcaaacaaaa agatgttgtc ggcaacatca ttcaggaatg
    29581 gctcggcgga tggttgagaa aaagggaaat tgaatttgat gtttcaacca atacccaaat
    29641 gcctcccgat ttctttttaa ataaaaaagg ccgcagcaga gaattgttgg aagtaaaggc
    29701 gttcaaccga aatgcctgcc cgggttttga tattgcagat tttaaaatgt attctgatga
    29761 aatcattcat aagccctata tgctggatgt agattattta atattcggtt acgatatgga
    29821 cgacaacggc aatgtaacca tcaaggattt atggcttaaa aaagtatggc aaattaccag
    29881 aagcatggat gggtgggcaa ttaatcttca agtcaaaaaa ggcgtggtgc ataaaatccg
    29941 cccgggtgtt tggtacagca taaataaaaa gaatatgcct atgtttgaat gcttggaaga
    30001 ttttgtttcc gcaattgaag aaaccgttta tcaaaacccg gcaacacggc ataacgcatc
    30061 cttatggaaa aagaaatttg aggaagctta taaaaaacac tacaaccgat cgatttccat
    30121 accccgttgg cacgaaattg cacacaaata caaaaagaaa taacccttca accatcaacc
    30181 cataaggaaa cttgaaatga ccaaacatat cgccatcctc cgaggcgacg gcatcggtcc
    30241 cgaaatcgtc gccgaaaccg tccgcgtact cgacaaattt atcgcccaag gcttggatgc
    30301 cgactacgaa tacgcgcctt tgggcggcga agcctacgac gaatacggtc atccttatcc
    30361 agaatttacg caaaacctct gccgcaaagc cgatgccgtc ctgcttggtg cagtcggttc
    30421 gccccaatac gacaatctcg accgtccgtt gcgccccgag cgcggcttgt tagcaattcg
    30481 taaggatttg aacctgtttg ccaatttgcg tcctgccgtt ttatatcccg aattagccaa
    30541 cgcctccacg ctcaagcctg aaatcgttgc cggtttggat attttgattg tgcgcgaact
    30601 taccggcgat atttatttcg gcgaaccgcg cggcatccgt gttttggaaa acggcgaaca
    30661 cgaaggctac aacaccatga aatacagcga aagcgaaatc cgccgcatcg cccacgtggc
    30721 cttccaatcc gcccaaaaac gcagcaaaaa agtctgctcc gtaggcaaag ccaacgtttt
    30781 ggaaaccacc gaactgtggc gcgaaatctt tgaagaaatc ggcaaacaat atcccgatgt
    30841 cgaactttcc catatgtatg tggacaatac cgccatgcaa ctcgtgcgcg cgcccaaaca
    30901 gtttgacgtg attgccaccg gcaacatctt cggcgacatc ctctccgacg aagcctccat
    30961 gctgaccggc tccatcggta tgctgccttc cgcttctttg gacgaaaacg gcaaaggcct
    31021 gtacgaaccg tctcacggct ccgcccccga catcgccgga caaaacaaag ccaacccgct
    31081 ggccaccatc ctctcgcttg ccatgctgct gcgttacagc ctgaacgacg aagcgcgcgc
    31141 gcaacaagtc gaaaacgccg tccaaaaagt gctgcaacaa ggcttgcgta ccggcgatat
    31201 ttacgaagaa ggcacaaaac tcgtttcctg ctccgaaatg ggcgacgcgg tactcgccgc
    31261 cttgtaaaaa acaaggggct gtactagatt atccctaaat tccacaccga tcccgcagga
    31321 tttttagctg ccgggacggt gtgccgaagt taaatcgaaa ttc
//
LOCUS       DAXFWM010000016        31149 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000016 DAXFWM010000000
VERSION     DAXFWM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31149
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.016"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            48..971
                     /locus_tag="ACFI8B_000723"
     CDS             48..971
                     /locus_tag="ACFI8B_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC3833997.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADDWRKRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILFVDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLRPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTKTDLPYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1050..1691)
                     /locus_tag="ACFI8B_000724"
     CDS             complement(1050..1691)
                     /locus_tag="ACFI8B_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC3833998.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(1746..2537)
                     /locus_tag="ACFI8B_000725"
     CDS             complement(1746..2537)
                     /locus_tag="ACFI8B_000725"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC3833999.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLKLPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            2703..2778
                     /locus_tag="ACFI8B_000726"
     tRNA            2703..2778
                     /locus_tag="ACFI8B_000726"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2736..2738,aa:Thr,seq:tgt)
     gene            2920..3711
                     /gene="panB"
                     /locus_tag="ACFI8B_000727"
     CDS             2920..3711
                     /gene="panB"
                     /locus_tag="ACFI8B_000727"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC3834000.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKVHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            3834..4670
                     /gene="panC"
                     /locus_tag="ACFI8B_000728"
     CDS             3834..4670
                     /gene="panC"
                     /locus_tag="ACFI8B_000728"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC3834001.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            4997..6835
                     /locus_tag="ACFI8B_000729"
     CDS             4997..6835
                     /locus_tag="ACFI8B_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC3834002.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            6975..7556
                     /locus_tag="ACFI8B_000730"
     CDS             6975..7556
                     /locus_tag="ACFI8B_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC3834003.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDRDGALAVDGKGNVYQAEGTE
                     DLSRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7566..8411
                     /gene="ispE"
                     /locus_tag="ACFI8B_000731"
     CDS             7566..8411
                     /gene="ispE"
                     /locus_tag="ACFI8B_000731"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC3834004.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8419..8494
                     /locus_tag="ACFI8B_000732"
     tRNA            8419..8494
                     /locus_tag="ACFI8B_000732"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8452..8454,aa:Gln,seq:ttg)
     gene            8507..8582
                     /locus_tag="ACFI8B_000733"
     tRNA            8507..8582
                     /locus_tag="ACFI8B_000733"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8540..8542,aa:Gln,seq:ttg)
     gene            8750..9733
                     /locus_tag="ACFI8B_000734"
     CDS             8750..9733
                     /locus_tag="ACFI8B_000734"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC3834005.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKGRGAEGVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            9800..10372
                     /locus_tag="ACFI8B_000735"
     CDS             9800..10372
                     /locus_tag="ACFI8B_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC3834006.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10498..11667)
                     /locus_tag="ACFI8B_000736"
     CDS             complement(10498..11667)
                     /locus_tag="ACFI8B_000736"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC3834007.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            11816..13342
                     /gene="ilvA"
                     /locus_tag="ACFI8B_000737"
     CDS             11816..13342
                     /gene="ilvA"
                     /locus_tag="ACFI8B_000737"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC3834008.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13398..14474)
                     /locus_tag="ACFI8B_000738"
     CDS             complement(13398..14474)
                     /locus_tag="ACFI8B_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC3834009.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGGGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(14471..15331)
                     /gene="cysW"
                     /locus_tag="ACFI8B_000739"
     CDS             complement(14471..15331)
                     /gene="cysW"
                     /locus_tag="ACFI8B_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC3834010.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAVLGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIQIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQTQGDSEEQAALVLGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(15520..16347)
                     /gene="cysT"
                     /locus_tag="ACFI8B_000740"
     CDS             complement(15520..16347)
                     /gene="cysT"
                     /locus_tag="ACFI8B_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC3834011.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAVEIGWDGF
                     RNTIAEPNVLAAVRLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDLPFA
                     LPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQPVL
                     EELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFIAGS
                     IPMISEILPLIITGKLEQFDAQGASAVALFMLLVSFVILFALNVLQWALGRRSGAKG"
     gene            16528..16860
                     /locus_tag="ACFI8B_000741"
     CDS             16528..16860
                     /locus_tag="ACFI8B_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834012.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            17187..17696
                     /locus_tag="ACFI8B_000742"
     CDS             17187..17696
                     /locus_tag="ACFI8B_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC3834013.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(17928..18509)
                     /locus_tag="ACFI8B_000743"
     CDS             complement(17928..18509)
                     /locus_tag="ACFI8B_000743"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC3834014.1"
                     /translation="MEHKLPQLPYGPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLALVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAA"
     gene            18672..20078
                     /gene="dnaB"
                     /locus_tag="ACFI8B_000744"
     CDS             18672..20078
                     /gene="dnaB"
                     /locus_tag="ACFI8B_000744"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC3834015.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSITKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYVPEEAKIED"
     gene            20233..20898
                     /locus_tag="ACFI8B_000745"
     CDS             20233..20898
                     /locus_tag="ACFI8B_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC3834016.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMATIALPNMSGWIASRRIASH
                     AEQIANLLRFSRGEAVRLNLPVYICPAQVKKDGTVDNQCDFGKKEQGMLAFGDKNDNK
                     AYDGDAADVFLRNVVLNDDIKDKRINYTFNHIAFGSSQPTADRVVWTFNQNGTFGYTK
                     DQHLASTSSFFYSDGYIQIVLTDARAVSDADRKFRSAVVLIDSSGRVEVCRKNDTRAV
                     CKH"
     gene            20930..21541
                     /gene="pilV"
                     /locus_tag="ACFI8B_000746"
     CDS             20930..21541
                     /gene="pilV"
                     /locus_tag="ACFI8B_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC3834017.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLWTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDLDSNKKNYSLYMGKQTLSAVDGKFMLDAE
                     KSKAQLAEEQLKRFSHELKNALPDAVAIHYAVCKDSSGDAPTLSDSGAFSSNCDNKAN
                     GDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            21538..22518
                     /locus_tag="ACFI8B_000747"
     CDS             21538..22518
                     /locus_tag="ACFI8B_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC3834018.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSIIVLIAVVSSYFT
                     SRELNDVANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTKDDIVDSSNQTQSN
                     LAKPGAKQENPLFSLKRSGMDKQLIPVAESIDIKYPGFIQRLNALVFQYGIDDLDASA
                     ETVVVSSCSKIAKPGKKISTLQEAKSALQITNDDKQNGNITRQKHVVNAYAVGRFGNN
                     EESLFRFQLDDKGKWGNPQLLVKKVKRMDVRYIYVSGCPEDEDAGKEEKFRYTNKFDK
                     SKNAVTPAGVEVLLDSGLNAKIAASSDNSIYAYRINATIRGGNVCANRTL"
     gene            22497..23105
                     /locus_tag="ACFI8B_000748"
     CDS             22497..23105
                     /locus_tag="ACFI8B_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC3834019.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYTADSKVTFSENCENGLCTAVNVRT
                     NDANEETFDNIVVKGKPTVEAVKRPCLAKSGKNSAGLCIDNKGMEYNKGVAGVSKMPR
                     YIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            23107..23580
                     /locus_tag="ACFI8B_000749"
     CDS             23107..23580
                     /locus_tag="ACFI8B_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC3834020.1"
                     /translation="MEQKGFTLIEMMIVVTILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQTIKSKLEIFVSGYKMNPKIAKKYSVSVKFVDAEKPRV
                     YRLVGVPNVGTGYTLSVWMNSVGDGYKCRDAASAKAYKEQLSGDGGCEALSNRKK"
     gene            complement(24192..24637)
                     /locus_tag="ACFI8B_000750"
                     /pseudo
     CDS             complement(24192..24637)
                     /locus_tag="ACFI8B_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            24803..25255
                     /gene="dut"
                     /locus_tag="ACFI8B_000751"
     CDS             24803..25255
                     /gene="dut"
                     /locus_tag="ACFI8B_000751"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC3834021.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     GTLLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            25333..26520
                     /gene="dapC"
                     /locus_tag="ACFI8B_000752"
     CDS             25333..26520
                     /gene="dapC"
                     /locus_tag="ACFI8B_000752"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC3834022.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDDLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPGNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQHASIAAWNDEQHVIDNRRMYQEKFERVIPILQQVFDVKLPDASFYIWLKVPN
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            26831..27610
                     /gene="yaaA"
                     /locus_tag="ACFI8B_000753"
     CDS             26831..27610
                     /gene="yaaA"
                     /locus_tag="ACFI8B_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC3834023.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQGHVRLLSGLYGLLRPLDLIQPYRLEMGTSFANLRGKNLYEFWGGIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            27693..27783
                     /locus_tag="ACFI8B_000754"
     tRNA            27693..27783
                     /locus_tag="ACFI8B_000754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27727..27729,aa:Ser,seq:tga)
     gene            27861..27947
                     /locus_tag="ACFI8B_000755"
     tRNA            27861..27947
                     /locus_tag="ACFI8B_000755"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27895..27897,aa:Ser,seq:cga)
     gene            28141..29334
                     /locus_tag="ACFI8B_000756"
     CDS             28141..29334
                     /locus_tag="ACFI8B_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3834024.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGLALWVYPTGGRSWKL
                     SFVQDGRQQTISLGRYPDFSLAEAREWREEVRRKRAHGENVVNKKVRADFAFEKVARD
                     WFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDTL
                     RKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERVKTKNMTALSPSELPRLIDFLEQ
                     RNEFAVYAGRVHIHPVTRFCIYWLLLTMTRIREAALMEWSELDGEVWRIPAERKKERR
                     GHDVPLSRAMRWVLDQARGLNVNGRFVFESVNFQGHINKESPRVAMRRSGLDTTAHGL
                     RSLARTYLREVLKVYNDVAEKLLAHSLGTRTQTAYNRSELWEERKDAPERWGNDVLRL
                     ADNGK"
     gene            complement(29690..29959)
                     /locus_tag="ACFI8B_000757"
     CDS             complement(29690..29959)
                     /locus_tag="ACFI8B_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834025.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(30154..30837)
                     /locus_tag="ACFI8B_000758"
     CDS             complement(30154..30837)
                     /locus_tag="ACFI8B_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFC3834026.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            30962..>31149
                     /locus_tag="ACFI8B_000759"
     CDS             30962..>31149
                     /locus_tag="ACFI8B_000759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834027.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
BASE COUNT         7463 a         7252 c         8610 g         7824 t
ORIGIN      
        1 tgatatgccg tctgaaaagt ttgtgaaaaa cggttacaat ccgccgcatg aaaaaacgca
       61 ataatcctct tccgctgttg aacggtgtca aacccagtta tttggtgctg ccgcatgaaa
      121 agcagtttta cgggctgccg ctgctgcatt ttctgtgcat ccgctttcct tttttgggag
      181 cggacgattg gcggaagcgg ttgaacagcg gttttgtggt cggttcggat ggtgcggcgt
      241 tggacgaaca ttctttgttc gagcccggta agacgatgtt ttattaccgt gaaaccggtt
      301 gtgagagcga gccgcgtatt ccgtttgaag aaaagatttt gtttgtcgat gaacatttga
      361 ttgtggtgga caagccgcat tttctgcccg tcatccccgg cgggcgtttt ttgcgggaaa
      421 cgctgctcac gcgtctgcgc ctgcgtcccg aattgcagca tttgaatgtt gaagacatta
      481 cgccgattca ccgcttggac aaggatacgg cgggtgtgat gctgctgtcg cacaatcctg
      541 ccacacgcgg ggcttatcag acgatgtttc aaaacaaaac ggtatggaaa acgtatgagg
      601 cgcttgcgcc gacaaagacg gatttgccgt atccgctcga tgtggtttcg cgtttggtaa
      661 ggggtgagaa attttttacg acgcaagagg ccgaaggcgt gccgaatgcg cacacgacgg
      721 tcgaattgat tgaaaacagg ggggaattca gcctttaccg ccttacgccg cataccggca
      781 agaaacacca gttacgcgtg catatgatgg gtttgggtat gccgctgttg aatgacgcgc
      841 tctatcccgt gccgtctgaa gcgggtagcg aggattatcg gaaacctttg aagcttttgg
      901 caaaaaagat tgcgtttgca gatcctttgt cgggtcggga aagggtgttt tgcagcgggt
      961 tttgcctgta atgagaggga acgcaaaccc gtatcggcag atgccgccgg atacgggttt
     1021 gatattatcg atgctgtctg aaaggggctt tatccgttca ggcatacggg tgcgaagcgg
     1081 aggatggtgt cgcgcaggac gatgagtttg ccgtgaaaga agtgcgtcga accggcgatg
     1141 gtaatgacgg gcaaatcttg cggttctgcc catttcaggg ctttttcgat ttcgacgacc
     1201 tcatcttccg cgccgtgtat catcagcgtt ttggcaacgt tgggaacggc ggacggctcg
     1261 gggcggtcgg tatagtggca gactgccgca ccgatgagca gcaataaatc gggtatgcgc
     1321 acttgtgcgg caaatgtggc gacatagccg ccgaaggaaa agccggataa ggcaaattcc
     1381 ggggcttcgg gatgttgggc gcgggcatag tcgatgacgg cgaggcagtc ttgcgtttcg
     1441 ccgcgtccgt aatcatgtgt gccttcgctg ccgcctacgc cgcgaaggtt gggaaggtag
     1501 cagtggaagc cgagcttgct caatgctttg gcggcggttt ggatgacttt gttggtgttc
     1561 gtcccgcctt ggagggggtt ggggtggttg atgacggcaa caccgcgtgc cggtacttgt
     1621 tcggacggga tatggatggt ttctaaaatg ccggcagggc cgggtatgtg tatggtttcg
     1681 ggtttcagca taatggtttc ctcttgattt tccgcatatt ccgatgcagc cgcgaagatg
     1741 gcggcttaaa ggtggaaatg cttgccgttg ttggggttgg acgctttcag gccggcgagc
     1801 atgcggtgga agtccaatcc gtatgcgttg ctcagtttgt cggcacgttt tttgagtttg
     1861 tctatgtttt gctctttggg gctgctttgg aaggccatta ccgcgacatt gccgtggctt
     1921 tcggcaggaa gtttcaatac gcgcccttca aaaacgctca acaaccgttc gatgaagcgt
     1981 tgatagcgtt tgtcgccgct ccaccagttg gtaacgaata tgccgtctga agagagtgcg
     2041 ttgcggcagt ctctgaaaaa cggttcttca accagcgtat cgatgatttg ttcgccgtcg
     2101 aatccgtcca ccaagatgat atcggtgttg tggcggaaga ctttgatgta ttccgcaccg
     2161 tctgcttcga taatttcaaa ttttccgccc tcgaaaggca actcaaacag gttgcgggca
     2221 atggcgatga cctgaggatt gatgtccacg gcggtttggc gcgtgtcggg caggtaggtg
     2281 tctgtccagc gtgcaaacga accgccgccc aagccgattt gggtgatatg ttgcggcagg
     2341 cgttccgcaa acagcagcca gcccatcatc gcgcggctgt aagagagcac cagctcggac
     2401 gggcggtcga ggttcatcga gctttgaacg gtgtcgctgc ccaagtgaag cgaacggata
     2461 ttgccttctt cggaaatacc gacttcggga aagccggatt ttgcaggacg caggcggcgg
     2521 tagggatgtc ttgccatcgg tgtgaacggt cggtttgaaa ggcggatatt ttatcagaat
     2581 gccgatgctt gttctgtttc aaattaattt tgttttaaat aagatactta gtgtattttt
     2641 atgagggggt cgaacaaatt tcttgccaaa gtgtgaagtt gcctgcataa ttcgccttct
     2701 ttgccggtat agctcagttg gtagagcacc tgacttgtaa tcagggggtc ccgagttcga
     2761 ctcttggtgc cggcaccaga tttgacagcc cgattgtgta aaacagtcgg gctgttttgc
     2821 atttgcggca cggtcggtgg cagggctgcg ggcatttcac tataatagcc cgtttcaaac
     2881 tgaaaaggcc gtctgaaaag ggcggggtaa caatatctga tgattactgt gaacacactg
     2941 caaaaaatga aggcggccgg cgagaaaatc gttatgctga ccgcttacga atccagtttt
     3001 gccgcgctga tggacgatgc cggcgtggat gtgctgttgg tcggggattc tttgggaatg
     3061 gcggttcagg ggcgacagtc gacgctgccg gtcagcctgc gcgatatgtg ctatcacacc
     3121 gaatgtgtag cacgcggtgc aaaaaatgcg atgattgtca gcgatttgcc gtttggtgca
     3181 tatcagcaga gtaaggagca ggcgtttgcc gccgccgccg aactgatggc tgccggcgcg
     3241 catatggtca aactcgaagg cggcgtgtgg atggcggaaa cgactgaatt tctgcaaatg
     3301 cgcggaattc cggtctgtgc ccacatcggt ctgaccccgc aatccgtgtt tgccttcggc
     3361 ggatataaag ttcaggggcg cggcggcaag gcgcaggcgt tgcttaacga tgccaaggtg
     3421 catgatgaag ccggagctgc cgtcgtactg atggaatgcg tgccggcgga actggcaaaa
     3481 aaggtaactg aaactgtttc ctgtccgacc atcggcatcg gggcgggcgc ggattgcgac
     3541 ggtcaggttt tggtgatgca cgatatgctc ggtattttcc cgggcaaaac ggcgaaattc
     3601 gtcaaaaact ttatgcgcgg gcaaagcagc atccaggctg cggttcgggc gtatgttgcc
     3661 gaagtcaaag ccaaaacctt ccctgctgcg gaacatattt ttgcagattg aacaaggtct
     3721 gctgccgatg ccgtctgaaa gccgtttcag acggcatttt gttttgccgt gcgcggcgcg
     3781 tataatcggc gcgttttgtc gggcaggaag cccgaaggat aaggattacc gtaatgcaaa
     3841 tcatacatac cattcgagaa ctgcgcacgt ggcgtgaaaa tataggaaag gtggcatttg
     3901 tgccgaccat gggcaatctg catgaagggc atcttgccct tgttcgcgaa gccagaaaac
     3961 gcgccgacaa cgttgttgtc agcatattcg tcaaccgctt gcaattcggt cagggcgagg
     4021 atttcgacaa atatccgcgt actttgcaac aggatgcgga caaacttgcc gccgaaggcg
     4081 ttgccgttgt tttcgcgccc gatgagaaag aactctatcc gaacgtggaa cagcgttaca
     4141 acgtcgaacc gccccatttg caaaacgaat tgtgcggcaa attccgcccc ggccattttc
     4201 gcggcgtggc gacggtggtg tccaagctgt tcaatatcgt tttgccggat gttgcctgtt
     4261 tcggcaaaaa agattaccag cagcttgccg tgattaaagg tttgaccgag gatttgaatt
     4321 tcgatatcga aattgtcccc gtcgatacgg ggcgggcggc ggacggtttg gcactctcca
     4381 gccgcaaccg gtacttgagc gttggggaac gcgccgaagc accgcgcctg taccgcgagc
     4441 tgcaggctgt tgccgaatcg ctgaagcagg gcggtttgga ttatgcgggt ttggagcggc
     4501 aggctgccga ccatttgacc gctgcgggct ggctggtcga ttatgtggaa atccgccgcg
     4561 ccgatacgct cgaaatggcg cgggcgggag ataagaaact ggtggtcttg gccgccgccc
     4621 gtctggggac gacgcgtctg attgacaatg tggaagtcgg cctgccttag cccgccggag
     4681 ccgggaatgc cgcctgaagc ggatttgcgt ttcagacggc attttgccgt cggctgtgct
     4741 tcggaataat gtcggaaaat agacggaatg aaataaattt ctttttgttc aacaaattaa
     4801 actgtcaaac caaataataa ggcaaggatt gtccggcatc gcatacaggc aaaccgacgc
     4861 aatctcaaaa cacagccctg ccttcgggcg ggttttgcaa atctacaaac cgtttggctt
     4921 gtgataaagt gacgccttgt ctagatactc aatatgccgt ctgaaggttc ggacggatgt
     4981 cggataaagg atgattatgt tacccgcccg tttcactatt ttatctgtcc tcgcagcagc
     5041 cctgcttgcc ggacaggcgt atgctgccgg cgcggcggat gtggagctgc cgaaggaagt
     5101 cggaaaggtt ttaaggaaac atcggcgtta cagcgaggaa gaaatcaaaa acgaacgcgc
     5161 acggcttgcg gcagtgggcg aacgggtcaa cagggtgttt acgctgttgg gcggtgaaac
     5221 ggctttgcag aaagggcagg cgggaacggc tctggcaacc tatatgctga tgttggaacg
     5281 cacaaaatcc cccgaagtcg ccgaacgcgc cttggaaatg gccgtgtcgc tgaacgcgtt
     5341 tgaacaggcg gaaatgattt atcagaaatg gcggcagatc gagcctatac cgggtgaggc
     5401 gcaaaaacgg gcggggtggc tgcggaacgt attgagggaa gggggaaatc agcatctgga
     5461 cgggttggaa gaggtgctgg cgcaatcgga cgatgtgcaa aaacgcagga tatttttgct
     5521 gctggtgcaa gccgccgtgc agcagggtgg ggtggctcaa aaagcatcga aagcggttcg
     5581 ccgtgcggcg ttgaagtatg aacatctgcc cgaagcggcg gttgccgatg cggtgttcgg
     5641 cgtacaggga cgcgaaaagg aaaaggcaat cgaagctttg cagcgtttgg cgaagctcga
     5701 tacggaaata ttgcccccca ctttaatgac gttgcgtctg actgcacgca aatatcccga
     5761 aatactcgac ggctttttcg agcagacaga cacccaaaac ctttcggccg tctggcagga
     5821 aatggaaatt atgaatctgg tttccctgcg taagccggat gatgcctatg cgcgtttgaa
     5881 cgtgctgttg gaacacaacc cgaatgcaaa cctgtatatt caggcggcaa tattggcggc
     5941 aaaccgaaaa gaaggtgcgt ccgttatcga cggctacgcc gaaaaggcat acggcagggg
     6001 gacgggggaa cagcggggca gggcggcaat gacggcggcg atgatatatg ccgaccgcag
     6061 ggattacgcc aaagtcaggc agtggttgaa aaaagtgtcc gcgccggaat acctgttcga
     6121 caaaggcgtg ctggcggctg cggcggctgc cgaattggac ggaggccggg cggctttgcg
     6181 gcagatcggc agggtgcgga aacttcccga acagcagggg cggtatttta cggcagacaa
     6241 tttgtccaaa atacagatgc tcgccctgtc gaagctgccc gacaaacggg aagccctgat
     6301 cgggctgaac aacatcatcg ccaaactttc ggcggcggga agcacggaac ctttggcgga
     6361 agcattggca cagcgttcca ttatttacga acagttcggc aaacggggaa aaatgattgc
     6421 cgaccttgaa accgcgctca aacttacgcc cgataatgca caaattatga ataatctggg
     6481 ctacagcctg ctttccgatt ccaaacgttt ggacgagggt ttcgccctgc ttcagacggc
     6541 ataccaaatc aacccggacg ataccgccgt taacgacagc ataggctggg cgtattacct
     6601 gaaaggcgac gcggaaagcg cgctgccgta tctgcggtat tcgtttgaaa acgaccccga
     6661 gcccgaagtt gccgcccatt tgggcgaagt gttgtgggca ttgggcgaac gcgatcaggc
     6721 ggttgacgta tggacgcagg cggcacacct tacgggagac aagaaaatat ggcgggagac
     6781 gctcaaacgc tacggaatcg ccttgcccga gccttcccga aaaccccgga aataatgcag
     6841 gcccatcctt tcagacggca taaggtttgc cgggaagccg gggcattcgg gcaaacggca
     6901 cgcagttcgc acgcgttttg cacggcacgc cgcacccgtc ggccggcagg atgacacccg
     6961 ttaaggaaac tctgatgaaa cacaccgtat ccgcatcggt catcctgctt ttgaccgctt
     7021 gcgcgcaatt acctcaaaat aacgaaaacc tgtggcagcc gtccgaacac atcagcagtt
     7081 ttgcagcgga agggcggttg gcagtcaaag cggaagggaa aggttcgtat gcaaatttcg
     7141 attggacata ccaaccgccc gtggaaacca tcaatatcaa cacccctttg ggcggcacgc
     7201 tcggacagtt gtgtcaagac agggacggcg cattggcagt ggacggcaaa ggaaatgtct
     7261 atcaggcaga gggtacggaa gacttgagca ggcagctggt cggtttcaaa ctgccaatcc
     7321 aatatctgca tatctgggcg gaaggcaggc gtgtggcggg cgcgccttac cgcatccgtt
     7381 cagacggcat attggaacaa tacggttgga caatcggcag aaccgccgac agtggggggc
     7441 aagtccgaac gttgcaactg aataacggaa atttgaacat caggctggtt ttcaccgaaa
     7501 ttggaatgcc gtctgaaacc gaaaccccgg aacgctgtgc ggcgcgcacg agataaggcg
     7561 gacagatgaa tattgcggac ggacggcagg cgtttcccgc acctgcaaaa ctgaatctcg
     7621 atttgaggat taccggcagg cgggaagacg gttatcacaa tatcgaaagc atattctgcc
     7681 tgatagattt gcaggatacc gtatatttga aaccgaggga cgacggcaaa atcatcctgc
     7741 acaatcctgt cggcggaata ccgcaggaag ccgatttgag ctaccgtgcc gcatcgttgc
     7801 tgcaaaaata tgcgcgcaac cttgccggcg tggaaatatg gctggacaaa aaaatcccga
     7861 ccggcgccgg cttgggaggg gggagttccg atgccgcaac ggttttgctg gtgttgaacc
     7921 gttggtggca gtgtggtttg acgcagtggc agctcattga tttgggtgcg gctttgggtg
     7981 cggacgtacc gttttttatt ttcggaaaaa atgcttttgc aagcgggata ggtgaaaaac
     8041 tgataggaat ggatattccg aaacagtggt atgttattgt caaacctccc gtccacgttt
     8101 ccaccgcaaa aattttcaca tacgaaggct tgacacggga ttctgcctca agcataatgc
     8161 cgactttcca aaacctgcaa ccgttcagaa acgatatgca ggcagtggta tttaaagaat
     8221 accctgaagt ttggaaagct tattccgagt tgtcgaaata cggttctgca atgatgaccg
     8281 gctcaggagc ctgtatattt gcagcatttc aagctaggaa tagcgcatac aatatatacc
     8341 gacaagtttc aggtttgtac gaggcatatt tggcagaggg tctttcaaaa catcctttgt
     8401 tgtccgtata aacattgttg gggagtcgtc aagcggttaa gacactggat tttgattcca
     8461 gcatgcgaag gttcgaatcc ttcctcccca gccaagccaa acaagttggg gagtcgtcaa
     8521 gcggttaaga cactggattt tgattccagc atgcgaaggt tcgaatcctt cctccccagc
     8581 cagacaaaag cgtgtaagcc tgcttacacg ctttttattt catagaaata aaaatattga
     8641 aacgcctttg tttgtgtcgg atgttgcagg tataatgccg ggcttggtac aagcggaggg
     8701 aagcattgtg ttttctgagc ggaagttaaa cataaaatca ggtgagaata tggctgcgta
     8761 cgacagtttg atggtattta caggcaatgc caatcccgaa ttggcacaac gtgttgtcag
     8821 gcatttggac atttctttgg gcaatgcttc cgtatccaag ttttcagacg gcgaagttgc
     8881 cgtcgaactg ttggaaaacg tacgcgggcg cgacgttttc atccttcagc cgacctgtgc
     8941 gccgaccaat gacaacctga tggaaatcct gacgatggcg gatgcactga agcgtgcttc
     9001 ggcaggtcgt attaccacag ccattccgta tttcggctat gcgcgccaag accgccgtcc
     9061 gcgttccgtc cgcgttccga tttctgccaa actggtggca aatatgctgt attcggcagg
     9121 gatcgaccgt gttttgactg tcgatttgca tgccgaccag attcaaggtt tcttcgatat
     9181 tccggtggac aatatttatg ccaccccgat tctgttgaac gacatcaagc aacagcggat
     9241 tgaaaatctg accgtcgtca gcccggacat cggcggtgtc gtccgcgccc gcgccgtggc
     9301 aaaatccttg aatgccgact tggcaatcat cgacaaacgc cgcccgaaag ccaatgtggc
     9361 ggaagttatg aacatcatcg gcgatattca aggcagaacc tgtctgattg tggacgatat
     9421 gatcgacact gcaaatacgc tgtgcaaagc cgccgtcgcc ctgaaagggc ggggggcgga
     9481 aggtgttcta gcctatgcca gccacgctgt attctccgga gaggcggtca gccgtattgc
     9541 ctcatccgaa atcgaccagg tggtcgtaac cgataccatt cctttgtctg aagcggctaa
     9601 aaactgcgac cgtatccgtc aggtaacgat tgcaggtttg ttggccgaaa ccgtccgccg
     9661 cattagcaat gaagaatccg tctcatatct tttcaatgaa gaagtgatga caggcagcat
     9721 gttgctgccg taaccccgat gccgtcttaa gctggtcgcg gccgatgacg acgattttac
     9781 ctaacttgga gtatttaaca tgacttatga aattcaagcc tctgttcgtg aagcacaagg
     9841 cactggtgcg agccgccgcc tgcgtcgcga aggccaaatc cccggtattc tgtacggtga
     9901 aggtcaagag cctgttgcca ttgccgtaga tcacaaaacc gtattttacg cattggaaaa
     9961 agaatctttc catactgcgt tgattaaatt gtctctgaac ggcgaaacca aagacgttat
    10021 cgtccgcgat ttccaaatgc acccgttccg ccgcgaagtc caacacatcg acttccaagc
    10081 tgtgaaagcc gatcaacttg tacgcatccg cgttcccctg cacattgtta acgctgaaaa
    10141 ttctcaagcc gttaaattgc aaggcggtcg cgtatctctg ctgaacacgg ctgttgaagt
    10201 gcttgcgttg cctgccaata tccctgcttt cttggatttg gattgtgccg aagtggttgc
    10261 cggcgacatt ttgcacttgt cagacatcaa actgcctgaa ggtgtagaaa gcgtttccct
    10321 gaaacgtaac gaaaatctgg ctgttgctac cgttaccggt aaaaagcgct aacttacttc
    10381 catcagcagg gcggcgcgta tgcaatgcgt accgccctgt tgttttatgc cgtctgaacc
    10441 gtgtttcaga cggcatttct ttatttgttg gaaaagcggg atatttgaaa cggcagatta
    10501 ctgccctgtc agacgcgtcc aaagcctttg ccaccggctt cttttttcta cgttttccag
    10561 tgcgacgatt tctttttcgg caatggtatg tccgttttgc ctgattttga tttttcctaa
    10621 aatctgccct ttttttaccg gggcgggaat cggctgtatg gtttccaaaa tctgttctgc
    10681 cattttcgct tctttatgtg gcagagtgat gtaggcttct ttgaggaagc ctgcgcggac
    10741 ggtttttttg ctgcctccgg aaatttggat ttgggcaacg gttttgcctt tcggatatat
    10801 tttgggcgta tcgaaggcct gcaatgcccg gttcagcagc ttgctgttgt ccgatgcgcg
    10861 ggtttccgcc gattccgaac ctagtgtgat gacaaggatg tgcctgccgt tgccggagta
    10921 tgacacggca aggttgtagc cgccgctttc tgtgtgcccg gctttcaggc cgtttacatt
    10981 gttgtcccta tataaaagga tattgcggtt gttttgttct atgttttcaa acttgaacga
    11041 tttgatggaa aacagcgggt aatattccgg aaagtcgcgc atcaatgctt cagacagcag
    11101 ggcgaggtct ttggcggtgg aaacctgtcc ttctctaccc aagcctgtcg ggtttttgaa
    11161 tacggtgttc ttcatgccca agcgtcgggc ttctttgttc atttgttgca caaaattttc
    11221 aatcgagccg ttgcccagcc ggtcggcaag ggttagggcg gcatcgtttg cgcatagggc
    11281 aatcatgcct tttaagagtt tgtcggtgct gaccgtatcg ccgggacgta caaacattct
    11341 gcttccttct gaagcccatg cggattcggg tatttttaag ttttcttcag attggatatt
    11401 tcccgatttc atgtttttga aaaccaaata tgcggtcatc agttgggtta gtgccgccgg
    11461 ttcgacaggg gtattggtgt ttttggcgga taaagtctgc ctgctttgaa ggtcgataac
    11521 gatgtgtgcc gccgtgaggg tttcgggtgt ttggagcgtg ggggcggcgt gtaccgtcgg
    11581 tctgttgggc gcgggcgatg cagccgttgc gtgagaaacg cctaagatga tgggaagaag
    11641 gacgggcagg attttatgtg ctgtcatgaa atattctaaa ttgtgtgagt gtttcagcct
    11701 gccgattata cgcttagggt gtctgatcgg gcggattttt cttgctttcg cgccgtcttg
    11761 ggcgtatggt tttgggtttt gcgattttaa taaaccgatt atcccgcatt gaattatgaa
    11821 cacgcccctt ccttattccg attacctcat ccgcatcctg acggcatctg tctatgatgt
    11881 agcggtcgaa acgcctttgg aaccggcacg cagcctttct gtacgtttga aaaacaacat
    11941 ccttttgaaa cgcgaagatt tgcagccggt tttttcgttc aaaatacgcg gcgcgtacaa
    12001 caaaatgtcc aagttgccga aagatgcgct cgcttgcggc gtgattgcgg caagcgcggg
    12061 caatcacgca caaggcgtgg cgttgtccgc acagcgtttg ggctgccgtg ccgttatcgt
    12121 catgccggag actacgccga aaattaaagt ggatgcggtt aaaagccgtg gcggcgaggt
    12181 ggttttgcgg ggtgtttcat acaacgatgc ctacgattat gcgatggagc tggcggaaca
    12241 ggaagggctg acctatatcg cgccgtttga cgatcccgat gtgattgcgg gacaggggac
    12301 ggtggggatg gaaattgtca gccagcatcc cgatccaatc cgcgccgtat tcgtaccgat
    12361 agggggcggc ggtttggcgg cgggcgtggc ggcatttatc aaacaggttc gtcccgaaat
    12421 taaagttatc ggcgtacaga ccaacgattc ctgctgtatg aagcagtcgg tcgaagcagg
    12481 cgaaatcgtc catttgaaag atgtcgggct gttttcagac ggtacggcgg tcaaagtcgt
    12541 cggaaacgga accttccgcc tctgcaaaga acttttggat gaaatcatta cagtcgatac
    12601 cgatgcggtt tgcggcgcgg tcaaggatat tttcgatgac acgcgcagca ttaccgagcc
    12661 ggccggcgcg ttggcgttgg cgggtctgaa agcctatatt gcccgagaag gtgcggaaaa
    12721 ccaaaccctg attgccgtta ccagcggtgc gaatatgaat tttcaccgtt tgcgccacgt
    12781 ttcggaacgg agcgaattgg gcgagggcaa cgaaggtatt tttgccgtta ccatccccga
    12841 agaacgcggc agcttcctta agtttgtcaa tatattggga aataggaata ttaccgagtt
    12901 caactaccgc tacggcgacg atgaaaaagc gcatatcttt gtcggacttc aagcggcagg
    12961 cccgcaggat ttggcggtta tcggcagccg gttggatgag gcgggattgc ccaatgtcga
    13021 tttgaccaat aatgagattg ccaaaatcca tatccgctat atggtcggag ggcggacgga
    13081 caaagtaggg cacgagcgtc tggtcagttt tgagtttccg gagcgtccgg gcgcgttggc
    13141 gcgctttttg aaccatatgc agggcggttg gaacattacg ctcttccatt accgcaacca
    13201 cggtgcggat tacgggcgga ttttggtcgg tatcgacgtg ccgccgcacg atgccgccgc
    13261 atttgacggt ttcttggaaa gtctgggata cagctatcac gaggaaacgc aaaatgccgc
    13321 gtacaagctg tttcttgcct gacgtttgac acgcaatgcc gtctgaaagc ctttcagacg
    13381 gcattgcgct ttcagggtta aatcgaatat tcaatcaact cgttttgaga aaaaacataa
    13441 acctgtttcg gaattaagtt taattttttg ccttcggcga ttgggtagcg cgcggcatcg
    13501 ctgccagcca gagtgatatg cacgtcttgt ttgccgtgtt tcaccaaaac gtgcgtcaat
    13561 gcgccgacgg cgtggatttt ttcgatttcg gcacggatca tcggggtttc gtgttcggcg
    13621 gcgatctgcc attcgtgcgg gcggatatag ccggtggcgg tttgctcctg ccatttgtat
    13681 tgcgcgtcca atttccacgc gaagccgttg taatgccaga tccctttttc gatgcgtcct
    13741 tcaaatgcgt cggcttcgcc gaggaactcg gtaacgaagg cgttttcggg tttgcggtaa
    13801 atggcttcgg cactgccggt ttgttcgatt ttgccatggt tcatcacgac gatttcgtcg
    13861 gaaacttcga gggcttcttc ttggtcgtga gtcaccagaa tgctggttac gccgaggttg
    13921 tgatgaatgt cgcgcagcca ggtgcgtaat tctttgcgca ctttggcatc cagcgcgccg
    13981 aagggttcgt ccaaaagcag gagtttcggt tcgaccgcaa gcgcgcgggc gagggcgatg
    14041 cgctggcgtt gcccgccgga gagttggtgc ggataggatt tggcgaggtg ggagagttgt
    14101 acgagtttga gcaattcttc gactttggcg cggatttgtc ctttggacgg gcgttcgggc
    14161 ttgggcaata cggtcaaacc gaaagcgacg ttgtcaaaca cattcatgtg gcggaagagg
    14221 gcgtagtgtt ggaacacgaa accgactttg cgctcgcgca catgcttggc ggttacgtct
    14281 tggccgtcaa agagaatttg gccgccgtcg gcgttttcca gtccggcgat aatgcgtaaa
    14341 agtgtggttt tgccgccgcc ggagggaccg agcagggaaa cgagtttgcc ggcggggacg
    14401 ttgaggttga tgtttttcag cgcgtgaaaa ttgccgaagc gttttttgtt taagttttga
    14461 atggtaatac tcatactgcg ttcctttcgg cggcggcgag ttttttgtct tgtaatttgg
    14521 tgatgatgtt ctgcaccgcc agcgtcgcca gtgctaaaag tgccaatacg ccggagaggg
    14581 agaatgcgcc ggtgaagttg tattcgttgt agaagatttc gaccaaaagc gggatagtgt
    14641 tggtttcgcc gcgtatgtgt cccgatacca cgctgaccgc gccgaactcg cccatcgcac
    14701 gggcgttggt gaggatgatg ccgtagagca gcgtccattt gatgttgggc agggtaacgc
    14761 gccaaaacat ctgccaaccg cttgcgccga gtaccaatgc cgcctgttct tcgctgtcgc
    14821 cctgtgtctg cattaaaggg atgatttcgc gtgcggcaaa gggaaaggta acgaacagcg
    14881 ccgtcaaaac aatgccggga atggcgaaga taatctgtat gccttgcgct tccagccggc
    14941 tgcccaacgc cgtgtgcgcg ccgaacaata agacgaacat caaaccggca accacgggcg
    15001 atacggaaaa cggcaaatcg agcagggtgg tcagcaactg cttgccgaga aagtcaaaac
    15061 gggtcaacag ccacgccatc gccacgccca agacggcatt gacgggaacg gcaatcagcg
    15121 cggtaatcag cgtcaatttg acggcagacc acgcttcggg atcgcttaag gatttcaggt
    15181 acaaatccca accgcctttt aaggcttcgt agaatacggc gacgagcggc acgaccagca
    15241 tcagcagcag aaagcccagc acggcggcaa tcagcaacat gcgcagccgg cgcggttcgg
    15301 tcaggttggg attggcggaa tagggtttca tggcggtggt gttctctggt ttgttgttta
    15361 aggtgttcgg atgccgtctg aaagggattg aggggtttca gctttcgtcg gtcggattct
    15421 tgaatccgac aaggttgctg aatcgcaaat gtttgtcgga tacaagtatc cgacctacga
    15481 attctgtgtt ttggggagcg gattcgtttt cagacgacct caacccttcg cacccgaacg
    15541 cctgcccaac gcccactgca gcacgttcag cgcaaacaga atcacaaacg aaaccagcag
    15601 cataaacaac gccaccgccg acgcgccctg cgcgtcgaat tgttccagct tgcccgtaat
    15661 aatcagcggc aggatttcgg aaatcatcgg aatgctgccc gcgataaaaa tcaccgaacc
    15721 gtattccccc gttgcccgcg cgaacatcat gcccgcgccg gtcaagagtg ccggcgtgat
    15781 ttccggcagc agcacgcggc ggaacgttgt gaaacggttt gcgcccaaag tcgccgccgc
    15841 ttcctcatat tcgcccgaca attcctccaa taccggctgc acggcgcgga cgataaaggg
    15901 caggctgacg acaaccagcg caatccaaat gccgacgggc gtaaacgcga ttttgatgcc
    15961 caaaggttcg aaaaaacggc ctatccaacc gttgggcgca tacagggccg ccaacgcgat
    16021 acccgtaacc gccgtcggca gcgcaaacgg caaatcaacc agcgcgttcg ccaggccctt
    16081 gcccggaaat tcatagcgca ccaacaccca agccaccagt gtgccgaaca cgacattggt
    16141 cagcatcgca taaaacgaca tccgcaaact cagccgcacc gccgccagca cgttcggctc
    16201 ggcaatcgtg ttccgaaagc cgtcccagcc gatttccacc gccatcatcg caaacggcaa
    16261 aaccaccaga agcgacaggc acaatacggt cagaccgagg ctgagtttga agccgggcag
    16321 tacgccgggt gttttgagcg gtaacatggt cggtaagggt ggaaaataaa ggcattactt
    16381 taacaatgcc gcctgaaaaa cggaaaaaat ggaaagtttg gtgcaaagac gaatttgtta
    16441 taaagcggtt ggcagttttt caagcgggcg cgatgttcta aaataacgct ttgttttgac
    16501 ggaaaaccat catataaagg aacacttatg cagattttat cttttcaggc ggacattgcg
    16561 gaacgtatgc tggaaggtac ggaaggcgag tcggtcaacg aaaacgcaca atttgtccgt
    16621 acggacaacg gctattggat tgcgtggcat gaaggcgtgg cggcactgct tgcgcccgat
    16681 acgccgccgg gcattccctg tttttgggtg gaaggggcgg aaagccttga agagttgtgc
    16741 gcgatggtgg aacgcggcga gtttgacgaa gtggaagagt ttgacggcga tgacgacgcg
    16801 tggctcgaag cggctaaaga ttgcggacac cacggcgacg cttgcgcctg cggacattaa
    16861 aggcatggca ggcttgccgc aaggggtgca aggctttgcc gttttttaaa taatcggcgg
    16921 attgcatctg tttgcaatgg gcggaacgct tccctttgta ttttattgaa taaaaattat
    16981 cttggtatta taataaggca gcatcgatta ttttgggact gcaacaaacg caaagcgttg
    17041 atttgcgccg attgcaaact tattatagca ggttgcggcg cggacttgat gggaattgtt
    17101 tatagagcat gatagtctga aacatattaa ttaaaaatac cattaatccg atttatattt
    17161 atttcaattt caatggaaaa acatcaatga caatgatttt aagcatttta agcctgtttt
    17221 ttatcattag actgttattt ttagccgtct ctattaaaca tgaaaaagcc ttgattgcca
    17281 aaggggcgaa acaatacgga aaaaccaatt ccacggtgct tgcggcagtt catacgcttt
    17341 attatttggc gtgttttgtt tgggtatggc tttctgacac tgcttttaat ggcatatcct
    17401 tgattggtac actgacggtg atggcttcat ttgtgatatt atcattgatt attaagcagt
    17461 tgggggagat ttggacggtt aaaatctata ttttgccaaa tcatcaaatt aatcgttcgt
    17521 ggttgtttaa aacattccgc cacccgaatt attttttaaa catcataccc gaactgattg
    17581 gcatcgcctt attatgtcaa gcgtggtatg ttttattgat tggcctgccc atttatttgc
    17641 tggtcttatt taagcgtatc cgacaagaag aacaggcgat ggcaacactt ttttaacccg
    17701 tttcatcaat taaaaatata tcgacaaaga gaaccgtcta tcaattaaat ccattcttat
    17761 cattggggca ggcttattgt cattgggcaa gccgtgacaa aagcccgaac ctgcaaaact
    17821 tatctataag gctgcatcag tgcgtattga agccccgcct tttttgacaa aaaaatgccg
    17881 tctgaaggct tcagacggca tttgttcggt ttggcaaagc cggcggctta tgcggcaaaa
    17941 cgtttggcga cttcgtccca gttgacgatt tcccaaaaac ctttcaggta gttggggcgg
    18001 ctgttgcggt agtcgatgta ataggcgtgt tcccacacat cgcaggtcag cagcggcgtg
    18061 ttttcagtgg tcagcggagt tgccgcgttg gaagtggaaa ccaaagccaa tccgccggca
    18121 ggggttttta ccagccacgc ccaaccggat ccgaaagttc cggccgcgca ggcattgaac
    18181 gcttcttgga atttttcgaa gctgccccat ttcgcgtcga tggcggcggc cagttcgccg
    18241 gcaggcttgc cttggccttt gggcgtgaaa cccagccagt agaaggtgtg gttccaagtt
    18301 tgagccgcgt tgttgaacac gccgcccgaa gattttttca caatctcttc caaaggcagg
    18361 ttttcaaatt cggtgccttt gatttgattg ttcaggttgg tgatgtaggt ttgatggtgt
    18421 ttgccgtagt ggaattccaa agtttctttg ctcagatgcg gggacaatgc gtccggccca
    18481 taaggcagtt gcggcagctt atgttccatt ttgtgctcct aatattgttt taaatgttgt
    18541 gttttggcag tgttgctgca aacgaatcgg cggctcacgc attctacctg ttttgagtga
    18601 tggaaaccaa ttaaacctgc tttgcgttat aatagaagat tgcaatttcg gcatgacaga
    18661 ttggatgtac catgaacgat tacgcagcta tgccgcctga aggccgtgag gtcggcgcat
    18721 tgtcgctgcc cccgcattcg atggaggcgg aacaatccgt tttgggcggt ttgatgctgg
    18781 aaaatccggc ttgggacagg attgccgatg tggtttcggg agaggatttc taccgccacg
    18841 aacaccgcct gattttccga tccattacca aattaatcaa tgagggccgt cccgcagatg
    18901 tgattacggt tcaggaagat ttgcagcgga acgaagaatt ggaagcggca ggcggatttg
    18961 aatatctgat tacactggcg caaaacaccc cgtctgccgc caacatccgc cgctacgccg
    19021 aaatcgtacg cgagcgttcc attatgcgcc aactcgccga agtggggacg gaaatcgccc
    19081 gcagcgcata caatccgcaa ggcagggacg cggggcggct tttggatgag gcggaaaaca
    19141 aagtgtttca aatcgccgaa agcaccgcca aatccaagca gggctttttg gagatgcccg
    19201 atttgctgaa agaagtcgta cagcgcatcg atatgcttta ttcgcgcgac aatcccgacg
    19261 aagtaaccgg cgtgccgaca gggttcatcg accttgataa aaaaacctcg ggtctgcagc
    19321 ccggcgacct gattatcgtc gccggccgcc cgtctatggg taaaaccgcg ttctccatca
    19381 atattgccga gtatgtcgcc attgaaaaac atttgcccgt cgccgttttc tcgatggaaa
    19441 tgggcggtgc gcagttggtc atgcgtatgc tcggctcggt cggacggctg gatcaaagcg
    19501 ttttgaaaac cggcaggctc gaagacgaac actggggccg tctgaacgaa gcagtcgtca
    19561 aactctcaga cgcgcccgtg tacatcgacg agaccccggg cctgactgcg ctcgaactgc
    19621 gcgcccgcgc ccgtcgtctc gcccgtcaat ttaacaataa gctgggatta atcgtcatcg
    19681 actacctgca actgatggcg gcatccggcc gttcggacaa ccgcgcttcc gagcttggcg
    19741 aaatttcccg ttccctcaaa gccctggcaa aagagttgca agtccccatc atcgccctgt
    19801 cgcaattgag ccgcactgtc gaacagcgca ccgacaaacg cccgatgatg tccgacctcc
    19861 gcgaatccgg tgctatcgag caggatgccg acctgattat gtttatgtac cgcgacgaat
    19921 actacaacca ggactcaccc atgaaaggcc ttgccgaatg tatcatcggc aaacaccgca
    19981 acggtcccgt cggtaaaatc ttcctcacat ggacgggaca attcaccaaa ttcgacaatg
    20041 ctgcctatgt tcccgaggag gcaaagatag aagattaaat ggctatataa aaatttatta
    20101 ggcggaatca ggcaaaatcg tttaaaatca tgcttagaga ttgccctaaa aaataaaacg
    20161 cggtcttgag gcatttttgc attcagtccg cagataattg aaaaatacat tacagttgac
    20221 gtcctattgc ttatgtgtac acgaaaacaa caaggtttca cgctaacaga gctgctcatc
    20281 gtgatggcca ttgcagccat tatggcgacg atagccctcc ccaatatgag tgggtggatt
    20341 gcatcacgcc gcattgccag tcacgcggag cagattgcca accttttgcg tttctccagg
    20401 ggcgaagccg tccggctcaa tctccctgtc tatatctgtc ctgcccaagt taaaaaagac
    20461 ggtacggtcg acaaccaatg cgacttcggt aagaaggaac aggggatgtt ggctttcggc
    20521 gacaaaaacg acaataaggc atatgacggc gatgctgcgg atgtttttct ccgtaacgtg
    20581 gtattgaatg atgatatcaa ggataagcgg attaattaca ccttcaatca tatcgctttc
    20641 ggttcgtctc agccgaccgc cgaccgtgta gtttggacat tcaatcaaaa cgggacgttc
    20701 ggttatacga aagaccagca tcttgcaagc acatccagct ttttttattc cgacggttat
    20761 atccaaatcg tgctgacaga tgcgagagca gtttcagatg ccgataggaa attccgttcg
    20821 gcggtggttt tgattgacag cagcggcagg gtcgaagttt gtcgtaaaaa cgatacgcgc
    20881 gccgtatgca aacattaatg actgttttag tcggtttggg aaataaaaaa tgaagaataa
    20941 tgattgcttg cgcctgaaaa atccccagtc cggtatggcg ttgatagaag tcttggtcgc
    21001 tatgctcgtt ctgaccatcg gtattttggc attgctgtcc gtacagttgt ggacagtcgc
    21061 ttccgtcagg gaggcggaaa cgcaaaccat cgtcagccaa atcacgcaaa acctgatgga
    21121 aggaatgttg atgaatccga ccattgattt ggacagcaac aagaaaaact atagtcttta
    21181 catgggaaaa cagacactat cagctgtgga tggtaagttt atgcttgatg ccgagaaaag
    21241 taaggcgcag ttggcagagg aacaattgaa gagatttagt catgagctga aaaatgcctt
    21301 gccggatgcg gtagctattc attacgccgt ctgcaaggat tcgtcgggtg acgcgccgac
    21361 attgtccgac agcggtgctt tttcttcaaa ttgcgacaat aaggcaaacg gggatacttt
    21421 gattaaagta ttgtgggtaa atgattcggc aggggattcg gatatttccc gtacgaatct
    21481 tgaagtgagc ggcgacaata tcgtatatac ctatcaggca agggtcggag gtcgtgaatg
    21541 aaacgtaaaa tgctaaacgt accaaagggc ggttatgatg gtatgaaggg ttttaccatt
    21601 gttgaatttc tggttgcggg cctgctcagt ataattgtcc tgatagcggt cgtatcgagt
    21661 tactttacat cccgggaatt aaatgatgtg gcaaacgagc gtcttgccat tcaacaggat
    21721 ttgcggaatg cggcaacatt aattgtccgc gatgcaagaa tggcggggag cttcggttgt
    21781 ttcaatatgt ccgagcatac taaagacgat attgttgatt caagtaatca aactcaatct
    21841 aaccttgcaa aacccggtgc caaacaagaa aatccccttt tttccttaaa aaggagcggc
    21901 atggataaac aactgattcc cgttgctgaa tccatagata ttaaatatcc gggttttatc
    21961 cagcgcctta acgcattggt tttccaatac ggtatcgatg atcttgatgc gagtgctgag
    22021 actgttgtag tcagcagctg ttccaaaata gcaaaaccgg gtaagaaaat atctaccttg
    22081 caagaagcaa agagtgcatt acagattact aatgatgata aacaaaatgg aaatatcacc
    22141 cgtcagaaac atgtggtcaa tgcctatgcg gtcggcaggt ttggcaataa tgaggaaagt
    22201 ttgttccgct tccaattgga tgataagggc aagtggggta atcctcagtt gctcgtgaaa
    22261 aaggttaaac gtatggatgt gcggtatatt tatgtttccg gttgtcctga agatgaagat
    22321 gccggcaaag aggaaaaatt cagatatacg aataaattcg acaaatccaa aaatgctgtt
    22381 acgcctgccg gggtggaggt tttattggat agcggcctta atgccaagat tgccgcttct
    22441 tcagacaata gtatttatgc ttaccgtatc aatgcgacaa tacgcggggg aaatgtatgc
    22501 gcaaacagaa cactttgaca ggaatcccga cttctgacgg acagaggggg tccgcactgt
    22561 ttatcgtgct gatggtgatg atagtcgtgg cctttttggt tgtaactgcc gcccagtcct
    22621 acaataccga acagaggatc agtgccaacg aatcagacag gaaattggct ttgtctttag
    22681 ccgaggcggc tttgagggaa ggcgaatttc aggttttgga tttggaatat actgcggata
    22741 gtaaggttac atttagcgaa aactgtgaaa acggcctgtg taccgcagtg aatgtacgga
    22801 caaatgatgc taatgaagag acttttgaca atatcgtggt gaaaggcaag cctaccgttg
    22861 aggccgtgaa gcgtccttgc cttgcaaagt ctggcaaaaa ttctgccggt ctgtgcattg
    22921 acaataaagg gatggaatat aataaaggcg tggcaggcgt cagcaaaatg ccgcgctata
    22981 ttatcgaata tttaggcgtg aagaacggac aaaatgttta tcgggttact gccaaggctt
    23041 ggggtaagaa tgccaatacc gtggtcgtcc ttcaatctta tgtaggcaat aatgatgagc
    23101 aataaaatgg aacaaaaagg gtttacattg attgagatga tgatagttgt cacgatactc
    23161 ggcatcatca gcgtcattgc cataccttct tatcagagtt atattgaaaa aggctatcag
    23221 tcccagcttt atacggagat ggtcggtatc aacaatgttc tcaaacagtt tattttgaaa
    23281 aatccccagg acaataatca gaccatcaag agcaaactgg aaatatttgt ctcaggctat
    23341 aagatgaatc cgaaaattgc caaaaaatat agtgtttcgg taaagtttgt cgatgcggaa
    23401 aaaccaaggg tatacaggtt ggtcggtgtt ccgaacgtgg ggacgggtta taccttgtcg
    23461 gtatggatga acagcgtggg cgacggatac aaatgccgtg atgccgcttc ggcaaaggcc
    23521 tataaagagc agttgtccgg agacggtggt tgtgaagcct tatccaaccg taagaaatag
    23581 aatgggtttg aagatgccgt ctgaaaattg gttttcagac ggcattttta tgggtatagt
    23641 ggattaacaa aaatcaggac aaggcggcga agccgcagac agtacaaaca gtacggaacc
    23701 gattcacttg gtgcttcagc accttaggga atcgttctct ttgagctaag gcgaggcaac
    23761 gccgtaccgg tttttgttaa tccactatac atcccgtcat tcccacgaaa gtgggaatct
    23821 agaaatttaa tgttgcggca ctagccaaaa aaaccgaaac cgaacggact agattcccgc
    23881 ctgcgcggga atgacggatt ttaggttggg gtcatttatt ggaaaaagca gaaaccgctc
    23941 cgccgtcatt cccacgaaag tgggaatccg gttttttgag tttcagtcat ttccgataaa
    24001 ttgccttagc attgaatgtc tagattcccg cctgagcggg aatgacgaat ccatccgtac
    24061 ggaaacctgc accacgtcat tcccgcgaaa gtgggaatcc aggatgcagg gttgggaaaa
    24121 ccgtgttatc cgataagttt ccgcaccgac aggtctagat tcccgcctgc gcgggaatga
    24181 cgattcacta taatgtagag gataaagcat gcgcccatat aagaaggcat attgaatgca
    24241 ggcaaaccgg ctgctttccg tttttggatt tcggagaatg ccatggcccg gctttcatca
    24301 cacataaaaa gcagcgcggg tacggttttt ttcagcggta tttctttcat gtgcgtggca
    24361 agcgccgccc ccatcaggat atgccgcgaa ttaatcataa aggttatggt ggcgataagc
    24421 agtatcggca gaggttccgc ccacaggttg accgtggcaa attcggagcc gccggcgaag
    24481 ttcatccccg tcatcagcag catttccagc cgggccatac ctttttgccc gcctgcattc
    24541 cgagtattaa tgcccaaggc aaaagcccaa tcagcatggg cgaactttct ttgatgccgc
    24601 gtatgaattc atcacgtgga gaggctgtgt gcgtcatgtt aatgtttata tatgaagaga
    24661 cggggcggta tcatacacag gcagaatgtt tttgacagtc gaggatggtt tcaaaacaaa
    24721 agcagtttgt tgtgtttcag cgtaaacacg gcttgtgtat aatctccaat ctttgaaacc
    24781 ggccgtatgc aggagcagga cgatgaatat tgaagtagaa atgaaagtgt tggacgagag
    24841 gatggcggat tttatccctg cctatgcgac ggagggttct gcaggtttgg atttgcgcgc
    24901 ctgtttggat gaggaagtcg ttttgcagcc gggcggaacg ttgcttgtgc cgacaggttt
    24961 ggcaatttat ttggcgaatc ccgcatatac cgccgttttg ctgccccgtt ccggcttggg
    25021 gcataaacac ggcattgttt tgggcaattt ggtcggctta atcgactccg attatcaggg
    25081 agaattgaag gtgtcgttat ggaacagaag cagcgaaccg tttactgtca agccgttcga
    25141 gcgtatcgca caaatggtta tcgtgccggt cgtacaggcg ggcttcaaac gtgtcgagga
    25201 atttgtcgga agcagccggg gtgagggcgg cttcggcagt acgggttctc actaaagata
    25261 tagaatgccg tctgaagggc acgtcagctt cagacggcat atcttccgat ataccgataa
    25321 ccggagaaaa tcatgaatac cttactcaag cagctcaaac catatccctt tgcccgactg
    25381 cgtgaagcga tgcagggcat ttccgcgcct gaaggcatgg aagccgtccc actgcatatc
    25441 ggcgaaccga aacacccgac accgaaagtc attacggatg cgctgaccgc ctcattgcgc
    25501 gagttggaaa aatatccgct gactgcgggt ctgcctgaac tgcgtcaggc gtgtgcgaac
    25561 tggttaaaac gccgttacga tgatttgaca gtggatgcgg ataatgaaat tttgccggtt
    25621 ttaggcagca gggaagccct gttttctttt gtccaaaccg tgttgaaccc tgtttcagac
    25681 ggcctaaaac ccgtaatcgt cagcccgaat cccttttatc agatttatga aggtgcgaca
    25741 cttttgggcg gcggtgaaat ccattttgcc aattgccccg cgccgtcttt caaccccgat
    25801 tggcgcagta tttccgaaga ggtttgggaa cgcaccaaac tggtgttcgt ctgctcgccc
    25861 ggcaatccca gcggcagcgt gccggatttg gacggatgga gagaagtttt tgatttacag
    25921 gataaatatg gtttcattat tgcctcggat gaatgctatt ccgaaatcta tttcgacggc
    25981 aacaaacctt tgggctgcct gcaagccgcc gcacagtcgg ggcgaagcag gcaaaaactg
    26041 cttatgttca ccagtttgtc caagcggtcc aatgttccgg gcctgcgttc cggttttgtc
    26101 gccggtgatg cggaactgct taaaaacttt ctgctttacc gaacctatca cggcagcgca
    26161 atgagcattc ccgtgcagca tgccagtatt gccgcctgga atgacgaaca acacgtaatc
    26221 gacaaccgcc gtatgtatca ggaaaaattt gagcgcgtta ttcccatttt gcaacaggtg
    26281 tttgacgtta aattaccgga tgcctcgttt tacatctggt tgaaagtccc caatggcgac
    26341 gatttggcat tcgcacgcaa tttatggcaa aaagccgcta tccaagtatt gcccggacgc
    26401 tttttggcgc gggataccga gcaggggaat cccggggaag gttatgtgcg tattgccttg
    26461 gttgccgatg tcgcaacttg tgtcaaagct gcggaaacca ttgtttccct atatcggtaa
    26521 agaataaaaa ataccgtctg aacttttgtt cagacggtat ttttcaatat tttacggttg
    26581 aatttgctat aacggtattt atagtggatt aacaaaaatc aggacaaggc ggcgagccga
    26641 agacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt
    26701 ctctttgagc taaggcgagg caacgctgta ctggtttaaa tttaatccac tatacttcac
    26761 ttttaatcgg cttgcccgca aacacgttta aacttaaaat ccccatgttt gacacaatac
    26821 cgagcggatt atgttttttg tcctttcccc tgcgaaaaac cttaacgaaa aagacccttg
    26881 ccctgtcagc gagtttaccc aacccgatct tttggcggaa tctgaaatcc tgatgcgcca
    26941 actgagggag cttgcccctc aacaaattgc agagttgatg cacgtttccg acaaaatcgc
    27001 acttctaaac gccgaacgaa atgcagcgtg gcacacgccg tttacgccgg aaaacgccaa
    27061 acaggcggtc tttatgttca acggcgatgt ttacgaaggt atggatgcaa acacattgaa
    27121 taccaatcaa atacaatatc tgcaagggca tgtacgcctg ctgtccggtt tgtacggtct
    27181 tcttcgcccg ttagacctga tacagcctta ccgtctggaa atgggaacgt ccttcgccaa
    27241 tttgcgcggc aagaacctgt atgagttttg gggcggcatc attaccaacc ttttaaatga
    27301 cacgcttgcc caagcaggca gcaatacgct tgtcaacctt gcctcacagg aatatttcaa
    27361 gtccgtcaac acgaaaaaac ttcgggcgcg gctgattacc ccgatattta aagacgaaaa
    27421 aaacggtaaa tataaaatca tcagtttcta tgccaaacgc gcgcgcggat tgatggtgcg
    27481 ctatgcggca gaacacaata ttaccgatcc tgaaatgctg aaaaatttta attacgaagg
    27541 ctacgcattc aatgacgcgg cttccaatga aagcgaatgg gtgtttatgc gttcggaaca
    27601 aataaagtga aaacaataaa ttaagtattt cccaaaaaaa gtgcttggca aaatgtataa
    27661 acttcattat tattcctact cttcaagaag acggaagcgt ggcagagcgg tttaatgcaa
    27721 cggtcttgaa aaccgtcgag ggttgatagc cctccgtgag ttcgaatctc accgcttccg
    27781 ccaatccttg aagtcaaaaa ccaaataaaa atgaagtagc ataaagcatc agcattttta
    27841 tttggtttta cttcattatc ggaaacgtgg cagagaggct gaatgcagcg gactcgaaat
    27901 ccgctgaggg tgcaaatcct ccgtgggttc gaatcccgcc gtttccgccg caaagcaaaa
    27961 ccgccctgat tcagggcggt tcttttttgt tcaggttgtg ttaattacta tataaaaatc
    28021 atcggtttac cctatcataa cgcatcaagg taaatcattt gcaatcttgc ggcatcttct
    28081 tattgcattt ttttatggta atgtatatgg taatttttgg ataagtggga aattaccata
    28141 atggcgaaaa tcattacgcc gctgtcggca aatcaggtta aaaatgcgaa gccgcgcgat
    28201 aagctgtata agttgtcgga cggggggctg gctttgtggg tctacccgac gggcggacgg
    28261 agttggaagc tgtcgtttgt gcaggatgga aggcagcaga caatttcgct ggggcggtat
    28321 cctgatttct cgctggctga ggcgcgggaa tggcgggagg aggtgcgccg aaaacgggca
    28381 cacggggaaa atgtcgtcaa taagaaggtg cgggcggatt ttgcttttga gaaggtggcg
    28441 cgtgattggt ttgtgcgttg gtcgaagggg cggtctgaaa agtatgccgg acaggttatg
    28501 cggaattttg aacggtgggt ttttccggct atcggcaatc ttgatatccg tcaagtcagg
    28561 acggcggacg tggtcggctg cctgcgtgtg atggaggcgc gcggtatcgt tgatacgttg
    28621 cgcaaaacga aaaacagtct gaagatggtg tttgcgtttg cggtcggttc gggaatgatg
    28681 gaaatcaacc ctgtcgcgca aatcggttcg ggtgtgtttg aacgggtgaa aaccaaaaac
    28741 atgacggcgt tgagtccgtc cgaattgccg cgcctgattg attttttgga gcagcgcaat
    28801 gaatttgcgg tttatgcggg cagggtgcat atccatcctg taacgcggtt ttgtatctat
    28861 tggctgctgt tgacgatgac gcggattcgg gaggcggcgt tgatggagtg gtcggagttg
    28921 gacggggagg tttggcgtat ccccgccgaa cggaaaaagg agcggcgggg gcatgatgtg
    28981 ccgctgtcgc gggcgatgcg gtgggtgttg gatcaggcgc gggggctgaa tgtgaacggg
    29041 cggtttgtgt ttgaaagtgt gaattttcaa gggcatatca ataaggaaag tccgcgcgtg
    29101 gcgatgcggc ggtcggggct ggatacgacg gcgcacggtt tgcgctcgct tgcgcgtact
    29161 tatttgcgcg aggttctgaa ggtgtataat gatgtggcgg aaaagctgct tgcccattcg
    29221 ttggggacga ggacgcaaac ggcttacaac cgctcggagc tttgggagga gcgtaaggat
    29281 gcgccggaac ggtgggggaa tgatgttttg aggcttgccg acaacggaaa atgatttttt
    29341 tgtgtgtttt gcgttatgtg gaaatgtaga taagaaagct gtttccgcat tatgtgaaaa
    29401 cgccctaatc gggcgttttt ttattgctgc aaccctaatg cttccaaaac ttcgcgggtg
    29461 tcccatacgg gggatgcgag gggggttcag acggcacggt atccgtccgt ctttttccat
    29521 acgcagcagt gtcgagtttg aaatggggtg gttgcggcag gttgcgtagg caatcagttc
    29581 gcggatggtc gggcggtcta tccttgcgcc cggtttggtg atgttcattt ttttactctc
    29641 ctgttcggtt taatcttgcg ccgtttttac ggctctgtcg tttgtctgcc tatttcttct
    29701 tcccgatttt tttatatcgc aacaagcttt ccggcgggaa gaatatgtca aagtctgttt
    29761 tcaaatcggg actgacgatg tcggcggcaa tcacgccgta gccggtggca aagctgtcgc
    29821 catcagcgtt atgaataact atatgcccgc ttttgattag aataacgcgg ccgaatccgg
    29881 atttccggat tccgcctttt ttcggggcgg ttgcaaatgc cagtgtttcc atccgccctt
    29941 gtacgtcgga agactccatt gcttcgccga ccgctttttt gacgggcgtt tattgaatac
    30001 ttcgtattcc atgtcttcaa tcctctttcc gttgattctg atttgtccgg gtttccgctt
    30061 ttttacggct tggcatgcgg caattgcgtc tttcatattc ggcatatcct ttccccaatc
    30121 cgccgaagtt tccgcatccg ctgccgtctc gattcatgtt tgccctccga tttgttttac
    30181 gaagcccgta ccgttcacgg catagtgtaa cttcacttgc ttacccgatt ccattcctcg
    30241 ggttaatgcc tcatattcca tttttgaccc ttgtaggatt gacgatttcg catctcttgt
    30301 ccttgcttcg gccatatccg gatattcttt ctttttgtat ccgtccataa tggctttttc
    30361 ttcatctgac atctcaaatt ttttgactgt ttcccaagcg ctcgcaatcc aaccgccgca
    30421 aaatcggtcg gcaagataag ttctgtgcga gggctttccc gcccggcagg ttttcaaaaa
    30481 tttgcggcgg tcggcggaaa tctgacgata gactacatca aaggcatagg ctgaggtttc
    30541 cgctcgattt ccaatgccgt aaaacatcat tgttttcccg cgttggtaag atttgcaccc
    30601 gaatatatcg gcaatcatat ttgcaacgcc ccattgccag cctgccaatt taaaagccat
    30661 tttccgatcg gctttctgtt cggagacttt tgataaggcg atatcctcgg catttacttt
    30721 gtacttttcc attaaagcct gcgcctgcct taacgcctgt gcggcttcat gttcatttgc
    30781 cgaccgaccc aaagccaaac atttcttgat tttgtctaag actttctctt tatccatatt
    30841 aaacccttta atttgtccgg tattttaatt tcgcaccctt gcccattcgt ctttcgcttt
    30901 gctttcgatt cggtcgggcc attcggctag ggctgatacg cttacgaact cggggctttt
    30961 tttgtttccg cctgccgaga ttaccggccg cggcagggta gttttgcaac aaggctttgc
    31021 aggcttcggg ctgtatttcc acggcgtgaa attcagacgg cttaatatac cgttcaagct
    31081 gcctgcttcc tgccgcgccg tcaaatacgc ccggatgttc gccgcagtat cggcggactt
    31141 tggcggcaa
//
LOCUS       DAXFWM010000017        30723 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000017 DAXFWM010000000
VERSION     DAXFWM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30723
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.017"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(2..466)
                     /locus_tag="ACFI8B_000760"
     CDS             complement(2..466)
                     /locus_tag="ACFI8B_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834028.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDGRLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="ACFI8B_000761"
     CDS             complement(543..3380)
                     /locus_tag="ACFI8B_000761"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC3834029.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="ACFI8B_000762"
     CDS             complement(3485..4744)
                     /locus_tag="ACFI8B_000762"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC3834030.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="ACFI8B_000763"
     CDS             complement(4823..6214)
                     /locus_tag="ACFI8B_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3834031.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVLLKGLQF
                     TMLGYALKQAFVPSKKHEDGEDHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVTNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGVEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            6420..6560
                     /locus_tag="ACFI8B_000764"
     CDS             6420..6560
                     /locus_tag="ACFI8B_000764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834032.1"
                     /translation="MWRAAEIINAADCHTSEIYSGLNLNRYSVASPCRTSCTVCGFVA
                     LS"
     gene            complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFI8B_000765"
     CDS             complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFI8B_000765"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC3834033.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7496..8176)
                     /locus_tag="ACFI8B_000766"
     CDS             complement(7496..8176)
                     /locus_tag="ACFI8B_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC3834034.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFI8B_000767"
     CDS             complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFI8B_000767"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC3834035.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFI8B_000768"
     CDS             complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFI8B_000768"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC3834036.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSEHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9944..10444)
                     /locus_tag="ACFI8B_000769"
     CDS             complement(9944..10444)
                     /locus_tag="ACFI8B_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC3834037.1"
                     /translation="MIRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFI8B_000770"
     CDS             complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFI8B_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC3834038.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYECQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFI8B_000771"
     CDS             complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFI8B_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC3834039.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFI8B_000772"
     CDS             complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFI8B_000772"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC3834040.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQILHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15560..16357)
                     /locus_tag="ACFI8B_000773"
     CDS             complement(15560..16357)
                     /locus_tag="ACFI8B_000773"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC3834041.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16360..17106)
                     /locus_tag="ACFI8B_000774"
     CDS             complement(16360..17106)
                     /locus_tag="ACFI8B_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC3834042.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFI8B_000775"
     CDS             complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFI8B_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC3834043.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17861..18853)
                     /locus_tag="ACFI8B_000776"
     CDS             complement(17861..18853)
                     /locus_tag="ACFI8B_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3834044.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18858..19694)
                     /locus_tag="ACFI8B_000777"
     CDS             complement(18858..19694)
                     /locus_tag="ACFI8B_000777"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC3834045.1"
                     /translation="MNIIDAIINLANNPVVGVESHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYIVGVVDKKKNLKHLAMVYGIDYCADAECYLK
                     IKNQIKEGIGNIGGIQFAETKELGRVNRIDPLNITYLRVRGMWGIENPWFVFNYIYQR
                     NMEKSFNFMAIINEDKWNSFNNTDKLLAIQDSKLAISDIKIKNPNNPARLRNAKLITY
                     HL"
     gene            complement(19803..20930)
                     /locus_tag="ACFI8B_000778"
     CDS             complement(19803..20930)
                     /locus_tag="ACFI8B_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC3834046.1"
                     /translation="MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTIGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(21000..21075)
                     /locus_tag="ACFI8B_000779"
     tRNA            complement(21000..21075)
                     /locus_tag="ACFI8B_000779"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21040..21042),aa:Thr,seq:cgt)
     gene            21182..21661
                     /locus_tag="ACFI8B_000780"
     CDS             21182..21661
                     /locus_tag="ACFI8B_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834047.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SDGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            21756..22379
                     /gene="rsmG"
                     /locus_tag="ACFI8B_000781"
     CDS             21756..22379
                     /gene="rsmG"
                     /locus_tag="ACFI8B_000781"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC3834048.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            22479..23252
                     /locus_tag="ACFI8B_000782"
     CDS             22479..23252
                     /locus_tag="ACFI8B_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3834049.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(23999..24583)
                     /gene="rnhB"
                     /locus_tag="ACFI8B_000783"
     CDS             complement(23999..24583)
                     /gene="rnhB"
                     /locus_tag="ACFI8B_000783"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC3834050.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(24651..26546)
                     /gene="mnmG"
                     /locus_tag="ACFI8B_000784"
     CDS             complement(24651..26546)
                     /gene="mnmG"
                     /locus_tag="ACFI8B_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC3834051.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(26719..28146)
                     /locus_tag="ACFI8B_000785"
     CDS             complement(26719..28146)
                     /locus_tag="ACFI8B_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3834052.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVNQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            28305..29297
                     /gene="pdxA"
                     /locus_tag="ACFI8B_000786"
     CDS             28305..29297
                     /gene="pdxA"
                     /locus_tag="ACFI8B_000786"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC3834053.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(29527..>30723)
                     /locus_tag="ACFI8B_000787"
     CDS             complement(29527..>30723)
                     /locus_tag="ACFI8B_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFC3834054.1"
                     /translation="RPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQ
                     AVPAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKIEVREAAGKTAGQKARADKAE
                     TRNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRN
                     ERGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERN
                     NQRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPL
                     IATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAAQEMPSETATFTAAA
                     EQARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQ
                     VETRQG"
BASE COUNT         6120 a         7832 c         9085 g         7686 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtccat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct tcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccatgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtt tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc tccgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttggcgtc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctggcggct ggcgcagccg
     1201 gtttggagct gcactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctgggga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcactgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgataccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggttacggt ttcggcgcgt
     2941 acgccgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc caagaatttt
     3061 tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggccgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcggt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtaaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaacacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgacaccgaa gctggtttgc actgcctgcg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcaaatgtg ccgaacagcg caaacgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggagttgg
     5821 tgacgcggta tttcaccgcc aacacgccct cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccaaaat accgcgcccg ggccaccggt taccaccgcg gtcgccacac
     5941 cggcgatatt gcccgtaccg atggtagccg acagcgcggt catcaacgct gcaaaatggg
     6001 aaatatcgcc ttcatggtct tcgccgtctt catgcttctt tgacggcaca aacgcctgtt
     6061 tcagtgcata acccaacatg gtgaactgca agcctttcag caaaacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccaaac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc acttttgtag tgtccggtaa tttcagcaca gaatattcaa taagacaata
     6301 tgttcttttg aaaaatactt ttggtttttt caccgaagac aggccggttc aagctgcgga
     6361 aattgtttgc aattatttaa aagcagaggc ggaggtcaca atgaaatgtc cgaatgggga
     6421 tgtggcgggc ggcagaaatc atcaatgctg ccgactgcca tacttctgaa atctatagtg
     6481 gtttaaattt aaaccggtac agcgttgcct cgccttgccg tacttcttgt actgtctgcg
     6541 gcttcgtcgc cttgtcctga tttaaattta aaccactata caaaatgatg catcgatcaa
     6601 acaacatacc gctttaaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
     6661 cattgaaagg gtgcggtcag cggatgatgc cgcgcgtcga ttgtgcgaag aagtcttgaa
     6721 atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtccgccttg gcttcttcaa
     6781 acggaatgcc gcgatggtag agggttttgt atacgtcttt gacggcggaa atctgttctg
     6841 cggtaaaacc gttgcggcgc ataccttcgc tgtttaggcc ggccggttcg gcgcggtagc
     6901 ccgatgccat aaagtagggc ggcacgtctt tgtgtacgcc tgcggcgaag gcggtcatgg
     6961 cgtagtcgcc gatgcggcag aattggaaga ccagcgtgta gccgcccaaa acgacgtagt
     7021 cgccgacggt aacgtgtccg gcaagcgagg cgttgttggc gaaaatggtg tggttgccga
     7081 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
     7141 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
     7201 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc ggaatttcgc
     7261 cgaggctggc aaattgaaaa atgcggttgt tttcgccgat ggtggtgtga ccgttgatga
     7321 cggcgtgcgg accgatttct gtattcgcac cgattcggac gttggggccg ataacggtgt
     7381 acgcgccgac tttgacgccg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
     7441 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaatcaca
     7501 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
     7561 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtaaata tttctagcgt
     7621 agccggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc aaacgattac
     7681 ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
     7741 gtgtagattt gggctttttt ggaaaaactt tcatcaatac ttttcctgca gcatcaaatt
     7801 gagcatcttt ccctcccgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
     7861 attggttgtg gtcaaacttt cctccgctat tattttgtat tttagataat gcaaccttga
     7921 ttaattcagc agattctttc gtaccatttc ttaaaaagca accttgatat ttcaatgcag
     7981 gattcgtttg tggttgtatg ctatcatata accggtttct gtctttaaac tcctgccgaa
     8041 agcaaatatc aattttgttt cttaaaacga tctcaaatat agaaacaaca tgaaaaagtt
     8101 ctcttgataa agataaattc aataaataca attcagctgc tttgtctttc ctaacggcat
     8161 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taattcacgc
     8221 taacccttga ttctttattt ttaaattgac gtttcaaact atatttgata tagttagata
     8281 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
     8341 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
     8401 gcacataatc acggcttcga cggcgacttg tccgtccact ttggcaacgg cgttgaattt
     8461 gccgatgccg cgccggctgg tcaggagttc gacttcaaag acgagttggt cgccggggat
     8521 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaaaaatt cgttttcctt
     8581 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcacgcc tgcgccatcg cttcgatgat
     8641 gagtacgccg ggcataacgg gcaggtcggg gaaatggcct tggaattggg gttcgtttat
     8701 ggttacgttt ttgatggcgg tcagggtttt catcggctcg aaggcggtga tgcggtcgag
     8761 ctggagaaac gggtagcggt gggggatgag tttttggatg tctttggctt cgatggggag
     8821 ttgtacgtcc atgtctgtcg tattccttga ataaagtcgg tttggattta ttgcgtgtct
     8881 ttgctgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
     8941 tggatgtaaa cggcgttgcg tgcccattct ttatgctcgg acatcgggaa gatgccggcg
     9001 atgtgtttgc cgctttcggt aatgctgtgg gtgacggacg tgccgccgcc gatggtggtt
     9061 ttgtcggcga tttcgatgtg tccgaccgta ccgacgccgc cgccgatgat gcagtagctg
     9121 cctatggtta cgctgcctga gatgccggtt ttggcggcga tgacggtgtg cgaaccgatt
     9181 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacggtggtg
     9241 tcgctcatcg cgccgcggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
     9301 gttaccgcgc cggtttgcgg gattttgaac cacgaatcgc cggcgaaggc gagtccgaaa
     9361 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgt gtctgccgag tgtgcagccg
     9421 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
     9481 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgttcgc gccgatgtag
     9541 gcgttcgcgc cgatttcaca gcttgcggga acggtcgcgc ccggttcgac gacggcggtc
     9601 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgcgcgac tttggcgaaa
     9661 tagagatagg gatcgtcggc gacaatcagg ttgcgccctt caaatccgtc tgctgctttg
     9721 gcggaaacga tgatcgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
     9781 gcgaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
     9841 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgcaggtg
     9901 gccggaatca tggttttcct gtttggtatg ccgtctgaaa gggttagcgg gcgttcattt
     9961 ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg tcctgcaaaa
    10021 tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg ttttgctgga
    10081 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    10141 tgcggaacgc ttcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtcct
    10201 taagtttgcc gccggcgagc tgcctttcca aatccaagcc ttcgcgttgc agtttttgca
    10261 attcgtcctg acgggcggaa aattcgccgt ccagcgtttt ttggatgttg cgcgcctgct
    10321 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    10381 gcgtgcctgt ggtgcagcat aaaccgatca gggtggcggc aaacgcgcgg gtcaaacgga
    10441 tcatggtaaa actccttcgg atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    10501 tttgcgggat tagaacgtcg tgccgagctg gaattggaag cgttggattt cgtcttccgg
    10561 ttttttcttc agcgggtagg cgtagctgaa tttcatcggt cccaaaggcg agagccaggt
    10621 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gcgcgttttc
    10681 cgagtaaacc gatttgttgt taccgttttc ggcggcggta taggttctgc cgtcccacac
    10741 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgt gcgtctttcg ccccgggcat
    10801 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    10861 gccgtattcg tcatacactt tcgggccgag cgtgccgctt tcgtagccgc gcaccgaacc
    10921 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    10981 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    11041 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    11101 atttacgccg gtcaggtagc cgcgcgtcgg ccataaggcg ctgtcggtct tgttgcgccc
    11161 ccagccgaca gtgcctttgt acagcaggcc tttgaagctg ccgtctgcgc cgtcggtttt
    11221 gccgtattgt ttgataaagt cggcatagcg tttgggtgct ttgttgtagg tgttgacggt
    11281 caggtgttcc gccgccagcc cgaaattgac gcggtcgtat tcggtaacgg ggatacccat
    11341 ccttacgccg ccgccggcgg tggtggtttt atattgtttg acgctggtcg atgctttgcg
    11401 cgggtcgaag gcttttccgt aaatatcgta gcccaggctg accccgtctg ccgtgaagta
    11461 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgcgagg cgcgcagggc
    11521 ggccgacttg cccgtaccga acaggttgtc ctgcgatacg ccggcggaca tgaccaagcc
    11581 ggtatcctga acccagcccg cgctcaagtc gagcgagccg gtggagcgtt cggtcaggct
    11641 catgttcaaa tcgactttgt cgggcgtacc ggcaagcggg acggcatcaa actgtacgtt
    11701 gtcgaagtag cccaaaagct cgacgcgctc tttggagcgt tgcagcttgg aggtgtcgta
    11761 aggcgcggat tccatttggc gcaattcgcg gcgcacgact tcgtcgcggg ttttgttgtt
    11821 gccggtgatg tggatttcgt tgacgtagat tttccggccc ggttcgatgt gcaggacgaa
    11881 atcgacggtt ttggttccgg cgttcggcag cggctgtacg ctgatttcgc tgtatgcgta
    11941 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcattc
    12001 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggccttgg ggacttcgtt
    12061 ggtgtcgcct tcaatcgaca ctttgcccca gcggaaacgt ccgccttcgt ggacggtgat
    12121 tttgatggtc tgcttggttt tgtcttcgtt ggtttggatg tcggtatcga ggatacggaa
    12181 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcgaattt
    12241 ctggcggtcg aaccggtcgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    12301 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    12361 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    12421 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    12481 caggccggcg actgcctggt tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    12541 gaacgattcg aggtttttct tgatggcgtc gttttgcagc attttggcgc cggtgatgtt
    12601 gagcgagccg atggtggggc gttcgataac ggtcagcaga agctgcccgt ccgcagtttc
    12661 gactcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    12721 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tgctcggctc
    12781 ggtacgctgc aagccttcga cacggatgtc ttggatggtg aagtcggcaa atgccaaagg
    12841 cgatatgccc aacatcatca gtgcggaggc aatctgtttc agtttcatcg taagttcctt
    12901 gtggtgcgga atgcggtttc agactgcatt ccgaaacgta aaatctaacc gatcagccgg
    12961 gtaacgtcgt tgaagaaggc ggccgccatc atcagcatca tcagggcgag cccgaagcgc
    13021 aaaccgatgt tttggacacg ttcgcccaaa ggtttgccgc gtatccattc gacagtataa
    13081 aacacgaggt gcccgccgtc caaaacgggg acgggcagca ggttcagcac gccgaggctg
    13141 atgctgacca acgctaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    13201 gcaatgtcgg caatggtcag cggcccggaa atatggctga cagaggcgtt gccgctgatt
    13261 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    13321 ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    13381 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcgggctg ttcgacagta
    13441 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggtcag ggtgattttt
    13501 ttgcccgggc tttggcgggt caggtttgcc cattcctgcc atgaggcgat gggtttgccg
    13561 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    13621 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    13681 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    13741 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    13801 gtttgcgcgc tgctccagtc ttggacggaa acgccgttga cggattgtat tttgtcgccg
    13861 ctttggaagc cggtgcgggc ggcaatggtg tcgggttcga ctgtgccgac atagggccgc
    13921 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    13981 gtcagcggac cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    14041 tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt gacgtagccg
    14101 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    14161 ttgccgaagc cgacggaaaa acgcacaacc ttgacgccgc acaacctggc gacgatgtag
    14221 tgtccgaatt cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtt
    14281 tgcaaatggt tttcctttga taacggtgtt cggacggtat cagcgcagtg tgccgataaa
    14341 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaac agtccttcta tatcgcccat
    14401 gccgtttgaa aagtcttgtg caagacagtg ggcgacggtt ttggcaatgt cggtaaactt
    14461 aatctgtccg tccaaaaagg cggcgacggc ggtttcgttg gcggcgttca atacgcaggg
    14521 cgcggctccg cctgcgttta tggtttcata ggcgaacttc aggcagggga agcggccgaa
    14581 gtcgggcttt tggaaggtca gcgcggacaa tgcgccgaaa tcgagtttgc cgacacccga
    14641 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    14701 cagttgcgcc agcacggagc cgtcgcggta gcgcaccata ctgtgtatca cggattgggg
    14761 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    14821 ttccaagccc ttgtttgcca tagtggctga atcgacggag attttgcgcc ccatacgcca
    14881 attggggtgt ttgaccgcct gctcgggcgt aatgctgtcg aacgtgctta aatcggttgt
    14941 taaaaacggg ccgccggaag cggtcaggat aatcgaatcg atgccgtgtt cgttcagacg
    15001 gtctgtgtaa tcgcgcggca aaacttggaa aatggcgttg tgttcgctgt cgacgggcaa
    15061 cactgccgcg ccgtttgcgc gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    15121 ttctttgttt gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    15181 ccccgccgcc ccgacgatgg cgcacatgac gccgctgact tcgtcggcag acgcaacgtc
    15241 aaccaatgcc tgcgcgccgt gtaaaatctg agtcgccgtg ccgtcgcgtt tcaacagggc
    15301 ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc ggaacgtttg
    15361 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    15421 ttcgggatgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    15481 tatggtcagg acttgtggtg tcataatgag gataacttta taccggatgc cgtctgaagc
    15541 gttttcagac ggcatagaat caatttaaaa ccgacattat cgccgcatag acgctgataa
    15601 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc aggttgctgc
    15661 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa caggtcgccg catacgctga
    15721 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    15781 agccggcact tcgtacggca gtcatgtaca ctgccacgca aaccgcaccg ccgattgcgc
    15841 cttcccagct cttgccgggg ctgattgccg gtgcgatttt gtgtttgccg agcgccttgc
    15901 cgctgaaata cgcgcaaacg tcggcaaccc agaccaaacc catcacggcg agcagcggca
    15961 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgagcaaaa
    16021 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccaca
    16081 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    16141 agccgcccgc ataggcaact acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    16201 cggttttgca caaaccggcc atacgggcat actcccacaa ggcggtcagg gcaatcagcc
    16261 cgcaaaatgc agcccacagc cattgcggcg cgtaaaacag catgcccagc atcagcggca
    16321 gcagccacat agcggttatt acccgttgtt tcagcatatt cagttccttt gctgtccgat
    16381 aggcagttgc tcggaagtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    16441 ggcatctaag gcggtttcgt cgaaatcggg ccacaggatg tcggtgaaat acagttctgc
    16501 atacgccatc tgccagagca ggaaattgct gatgcgcgtt tctccgcccg tgcggatgaa
    16561 caaatccggt tccggcgcgt cgcccagcat caagtgtttg gccagcgtgt cttccgtaat
    16621 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc agcggccgcc
    16681 gtaatccgca gcaatgctca gggtcagacc ggtattattt gccgtcaagg cttccgcctc
    16741 ttcgatacct tgcagaatct gccgattaaa gcgttcgcgg ctgcccaata ttttcaggcg
    16801 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    16861 caggaacgaa acttcgtctt cggggcgacg ccagttttcg gttgaaaagg caaacacggt
    16921 cagatattgc acgcccagtt tggcgcaatg cttcaccata ttttccaacg cgtccaagcc
    16981 gcgtttgtgt cccattatac gcgggagaaa acgttttttc gcccaacggc cgttgccgtc
    17041 cataattacg gcgatgtgcc tcgggatggc ggtgtgttcc aaaatggtct gcgtgctgct
    17101 cttcatatct gcctttcgcg gttcggcgtt caaatgccgt ctgaacgccg cgccgtgaga
    17161 tttaaaccgc catcaggtct tcttctttgg cagccaaaac cttatcggct tcggcaatgt
    17221 acttgtcggt cagtttttga accgcctctt cgccgcgacg tgcctcgtct tcggaaattt
    17281 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatag
    17341 agacgcgtcc ttcttccgcc tcgccgcgca cgactttaat caggtctttg cggcgttcct
    17401 cggtcagcat gggcatcggc acgcggatca aatcgccgac agctgccggg ttcagtccca
    17461 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    17521 cgccgatggt gcgcgcgtcc aaaagggtta cgttggcaac ctggctgacg gggaccatgc
    17581 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    17641 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    17701 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaggcggg cagccgttcg
    17761 aacgcgctcc aagccgttca gacggcataa agaccgttaa ctgcgaatag taccgttatt
    17821 cgggcatgac gacaaggcag atgcctgtag gaagggtgtt tcacctttct agggtttttt
    17881 taattgccgc tgcaatttcg taggcaaggt tgacggggac ggcgttgcca atcattttgt
    17941 atgcgtcgtt gacattttga tagatgaatt tgaagttgtc gggaaagcct tggattcttg
    18001 caacttcgcg taccgtcatc ctccgatata gtgtttcttt gccggcagca aaacggtagt
    18061 cgtttgcccc gtgcttttcc atctttgggg cttgcgggtg cagttggcac tgcctgcctg
    18121 aagcttggac ggtaaaaccc tgttcatccc acgctttaac tcggttccgg ctcataaaaa
    18181 taggggaaaa actgccggta aaatattcgt tgttgttgac tgcgtcgggg ttggtcttgt
    18241 tttgcggggc ggaaggtacg gctgtgtcct gcaaatccca aataacgtct ttcaatgtaa
    18301 tcttgtcttt gtcttcgacc gtcgaacctt ttggaaaaga aaattttatt tccaagtctt
    18361 tacggaaacc gatgtagaag acccttttgc gttcctgtgc tacaccgtag tctttggcgt
    18421 tggccatagt caaggttacg tcgtatccgc atccgtcaaa cattttcagc aggttttgta
    18481 cggctccgtt gtggcggttt gccagcattc cgctgacgtt ttccgctaaa aagaattttg
    18541 gctgtttgct tttcaaaata cggatgtagt cgaaaaacaa ctgtccgcgc gcatcgtcga
    18601 tgccgcgcaa agctcccgct tcagaccaag actggcaggg tgggccgccg ataatcccgt
    18661 cgatttcttc agggaaatct tcttctttaa tcttgcgtat atcgccttct atcaaatggg
    18721 tctttggatg gtttgccttg aaggttgccc aaatggtttt atcgtattcg ttggcggcgg
    18781 ggatttcaaa tcccgctttt tcgaaaccca aatccaaacc gccgcaaccg ctaaacaaac
    18841 taatgatttt cataggatta taagtggtag gtgatgagtt ttgcatttct taatcgcgcc
    18901 gggttgttgg gatttttaat tttaatgtcg gaaatggcca gtttggaatc ctgtatggca
    18961 agcagcttgt ctgtattgtt aaaactgttc catttgtctt cgttgataat ggccatgaaa
    19021 ttgaaacttt tttccatatt ccgttggtaa atatagttga agacaaacca tggattttcg
    19081 attccccaca taccccgtac tctcagataa gttatgttga gcggatcaat gcggttgacc
    19141 ctgcccagct ctttggtttc tgcaaactgt atgccgccga tgttgccgat tccttcctta
    19201 atttgatttt ttatcttcaa atagcattcg gcatcggcgc agtagtcgat tccgtaaacc
    19261 attgccaagt gtttgaggtt tttctttttg tcaacgacgc cgacaatata gataatgtct
    19321 ttctcttccc atttttccgc atctttgcag gcttttgtaa gcatgctgtc atcaacagag
    19381 agctttgatt ttgggtggct gctattcagc gccagtgcgg aatctttact ttcaattttc
    19441 ttaacttcga tggcatcccc gtttcgcaac atggcatcag gagggttgga gttgttgccc
    19501 aaatatgaga atacttttgc gtgtcgggct atgcgttggg tttcatttag gttgaaactg
    19561 ccggaaaaca agtcttttac atattcttcc aaagcatcac ctgcctgatt ggcacgattg
    19621 ttgctttgag aatgggattc gaccccgaca acagggttgt tagccaaatt gatgatggca
    19681 tcgataatgt tcatggtttt tccttggggt tttgcggcag attatagcaa aaccgtcagg
    19741 gtgtttatcg gaccgatgcc gtctgaagcg gcaggcgttt cagacggcat cgtgtccggc
    19801 cgtcagccgt gttcccgtgt ttcaagcagg ctttggcgca ggtgttggcg ttcgtgggca
    19861 tccagccatt tgcggcgggt gcgttgcagc aggatgacga gggcggaaat ttcctgacgc
    19921 atattggtgc tgagccagag gaagccctgc cattggtagt ggaggtgttc ggcgagggct
    19981 tccagttcgg ggttgatggc ggtgtcgatg cggatgcggc gggcgtgtct gccgttgatg
    20041 agggcggcgg tttgttgcag gtcggtttgg agcagtgtga agtggcggtc gagcagccgg
    20101 atttcgctgc cgttgagttt gggagattgc agcttggcgg cggtggtcag gagcagctcg
    20161 gtggtgttga cgattttgcg gtgggcgtgc tgcatggctt ccatcatgga ggggctgatg
    20221 cggctttcgc ccgatgtggc ggcgaggtgg ctgcggcttt tgaccatgcg tgcgttgatt
    20281 tggcgcattt tgaccatatt ctgctccaaa cgttcgcgcg tcatacgcct gccgttgctg
    20341 atttcggcaa tcattttgct gcagtcggcc aggttgtcgg caagcatgaa acgccacatc
    20401 agtgtggatt tcagcggcag cagtttggcg gcggcaatgg cgatggcggc gccgatgagg
    20461 acgttcatcg cgcgcatcag gccgctgtcg agccattcgc tgccgttgtc gccgatgagc
    20521 atgcacatcg tcagccccgc cagcataggg acgtagccgt ttttgccgac cgccgcccag
    20581 ccggccagtg cgcttgccgt gccgatggtc aggtagaaga ggaggttgcc gtggaaataa
    20641 tgctggttca gccataaaac gcccaaaccc gcgcccagcc cgatgaccgt accgagcata
    20701 cgttccaccg ccttggagta aatcgcgcct tggaactgga gcatgccgag gacgacgaag
    20761 acggtcatcc ctatccattc gccgtgttgg aggtggagta gccgggcgag tgcggtggcg
    20821 aacaggacgg ctccgccgag ccgcacggca tgtatgaggc ggcggtggcg gtagcgttcg
    20881 taggagttga gccagcggcc ggaaaggcgt ttgcgttgcg aggagttcat atcggatgcc
    20941 gtctgaagcg gaaatgtgaa aaagcacagg cttcccgagg gagggtctgt gcttggtatt
    21001 ggtgccggag aagggaatcg aacccccgac cttcgcgtta cgaatgcgct gctctaccga
    21061 ctgagctaca ccggcgtttt tcgttatgat atatataaac ggttgtttgt gcaacttttc
    21121 gggcgggcgg caaggcggtg cgcggtataa tgcggcctgc ttcggaagag gggggacggc
    21181 gatgtttgtg aacgagaaat atccttatgc gaccctgttt gcggggctgg tgtttttgac
    21241 gctgccgttt gcgttggcgg tgcatgatgc ctttgcatgt gcgttcggac gggcggggct
    21301 gctggtgtcg gtgtcggacg gcggattcgg ccggcgtggc ggttgggacg gcactgtttg
    21361 gtttgtgttc ggtgtgtttg cgtttttgaa tgtggttgtg tcggcgggcc tgacgaaact
    21421 ggcgtacaaa aagatgatgc ggcggcattc gcgttacgcg ctgtttctgt cgggcgtggc
    21481 ggcttgcgcg gcggtggctt ggattttcaa gctgttgttg ggcagtgccg ccttgggcgg
    21541 gctgagcggg gaggcggtgt cggaatatgc gtttgccgtg tggctggtgt ctatgctgac
    21601 gctgccgaaa cgcctgacgc gcgcgccggt gcagcctgtg gtgttccaca ggaaaaaata
    21661 ggacggaacg gaaatgccgt ctgaaacccg atacgcggtt tcagacggca tgtttttccg
    21721 ctaacattac gcctgaatat ggacaggaag cagatatgga acgtaaagaa cgcctgcgtg
    21781 caggcattgc cgcgatgggg ccggatattt cggaaacggc gcaggacagg cttttggcct
    21841 atgtggattt gttgaaaaag tggaacaaaa cctacaatct gaccgccctg cgcgacgagg
    21901 aaaaaatgat tgtccatcat cttttggaca gcctgacgct gctgccctat atcgaaggcg
    21961 cacagacgat gctggatgtc ggttcgggcg gcggccagcc cggcattccg gcggcggtgt
    22021 gccgtccgga tgtgcagata accttgttgg atgcgaatac gaagaaaacg gcttttttgc
    22081 ggcaggcggc tatcgagttg gggctggaca atgtgcgcgt ggtgtcgggg cgcgtggagg
    22141 cggtttcgga cgtgcgtgcc gacgtggtta ccagccgtgc gtttgcggaa ctggcggatt
    22201 ttgtgtcgtg gacggcgcac ctgttgaaag acggcggcta ctgggcggcg atgaagggcg
    22261 tatatccgca gggggaaatc ggccgcctgc cgcaggatgt gtgcgtcgaa aaagtccaaa
    22321 ggctcgacgt gccgggcttg gatgcggaac gccatatcgt catcctgagc aagcgttgag
    22381 cgcgcttcag acggcatgaa tacctttttt gtgcggataa aggtaaaatt ccgcactgtt
    22441 tttctttttt tcaacatcag acgggacacg ggcgggacat gagtgcgaac atccttgcca
    22501 tcgccaatca gaagggcggt gtgggcaaaa cgacgacgac ggtaaatttg gcggcttcgc
    22561 tggcatcgcg cggcaaacgc gtgctggtgg tcgatttgga tccgcagggc aatgcgacga
    22621 cgggcagcgg catcgacaag gcgggtttgc agtccggcgt ttatcaggtc ttattgggcg
    22681 atgcggacgt gcagtcggcg gcggtacgca gcaaagaggg cggatacgct gtgctgggtg
    22741 cgaaccgcgc gcttgccggc gcggaaatcg agctggtgca ggaaatcgcc cgggaagtgc
    22801 gtttgaaaaa cgcgctcaag gcagtggcgg aagattacga ctttatcctg atcgactgtc
    22861 cgccttcgct gacgctgttg acgcttaacg gcttggtggc ggcgggcggc gtgattgtgc
    22921 cgatgttgtg cgaatattac gcgctggaag ggatttccga tttgattgcg accgtgcgca
    22981 aaatccgtca ggcggtcaat cccgatttgg acatcacggg catcgtgcgc acgatgtacg
    23041 acagccgcag caggctggtt gccgaagtca gcgaacagtt gcgcagccat ttcggggatt
    23101 tgctttttga aaccgccatc ccgcgcaata tccgccttgc agaagcgccg agccacggta
    23161 tgccggtgat ggcttacgac gcgcaggcaa agggtgccaa ggcgtatctt gccttggcgg
    23221 acgagctggc ggcgagggtg tcggggaaat aggtcaatcc aaatcgggct gcccgtgcct
    23281 ttatgctgtt tggccgggtg cgttaatatg gcggattaaa ataaaaatac ttatatcgtc
    23341 atttatagcg gattaacaaa aaccggtacg gcgttgcccc gccttagctc aaagagaacg
    23401 gttccctaag gtgcccaagc accaggcgaa ccggttccgt actatctgta ctgtctgcgg
    23461 cccgccgcct tgtcctgatt tttgttaatc cgctatatcg tcattcccgc aaaaacaaaa
    23521 aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc aggcgggaat ctaggtctgt
    23581 cggtacggaa acttatcggg aaaaacggtt tttccaaccc tgagactccg gattcctgtt
    23641 ttcgcgggaa tccggttttt tgagtttcag tcatttttga taaattcttg cagctttgag
    23701 tttctagatt cccgcttttg cgggaatggc ggtttggaag ttacctgaaa ttcaaaaaaa
    23761 aaaaacggaa accggacgga ttggattccc gcctgcgcgg gaatgacgga ttttaggttt
    23821 ttttgatttt ctgtttttcg cgggaatgac ggttgggaag tttcccgaaa cttaaaaaca
    23881 actgaaaccc aaaaaactaa attaccgcct gcaggggaat gacggttcgg gttctttctc
    23941 tttgaagttg cgatgctgga aatgccgtct gaagttcaga cggcattttt gtgccggttt
    24001 aaaacaaggc ctgctgcgcg agcaggtttc tgacgggggc gaagtcgcgg cggtgttcgg
    24061 gcagcacgcc gtattgtttg agtgcttcca gatgctgctt tgtgccgtaa cctttgtgtt
    24121 tgtcgaaacc gtattgggga cggcgttgcg ccagtgcgta catttccgca tcgcgtgcgg
    24181 tctttgccaa aacggatgcg gcggagattt cgatgatttt gctgtcgcct ttgacgactg
    24241 cttcggcagg gatgcctaaa tgttcgggaa tgcggttgcc gtcgatgaat attttttcgg
    24301 gacgcgcagc caaaccgtaa acggcacgtt tcatggcgag catggtggcg tgcaggatgt
    24361 tgaggcttgc gatttcttcg ggcgtggagg cggcgacgtg ccacgcgacc gcctgttctt
    24421 taatcatttc ggcaagcgcg tcgcgttttt tctcgctgag ttttttggag tcggtcagtc
    24481 cgggcaggtc gaatgtttcc ggaaggatga cggcggcggc aaacacgctg ccgaccaaag
    24541 gtccgcgtcc tgcttcgtcc acgccggcgg tcagtatgtg catgatgttt cctgtcggga
    24601 tggtacgatg ccgtctgaaa atggtttcag acggcatcgt accgatgtgt ttatttcgcg
    24661 tctttaaacc cgcgcttcaa atgcaccatc agcagtgcga cggcggcagg cgtaacgccg
    24721 gaaatgcggc ttgcctgccc gacggtttcg ggtttgtgct ggttgagctt ttgttgcact
    24781 tcggccgaca agcctttgac tctgccgtaa tcgatgccgt cgggcagctt taaggtttcg
    24841 atgtcgcggc ggctgtcgat ttcttcgttt tggcggtcga tatagccttg gtatttgact
    24901 tggatttcga cttgctcgat gacttcggca gagaggtttt cagacggcat cgcgccttcg
    24961 agcgtcatca gcgcggcgta atcgaggttc gggcggcgta acaaatcgtg caggttggct
    25021 tcgcggctga gtttttgtcc gaacacgcgg atttgttcgt cttcggcgag tttttgcggc
    25081 gtgtaccacg ttgttttcaa acgttggatt tcgcgttcga cggcttcgcg tttttcgttg
    25141 aacatgcgcc attgcgcttc acccaccaag ccgattttgt agccgtcttc ggtcaggcgc
    25201 atatcggcgt tgtcttccct gagttgcagg cggtattcgg cgcggctggt aaacatccgg
    25261 taaggttcgt tcaggccttt ggtgatgagg tcgtccacca atacgccgag gtaggcttgt
    25321 tcgcggcgca gcaggagcgg atcttgtccg cggacgtatt gcacggcgtt cgcgcccgcc
    25381 agcaatcctt gcgcggcggc ttcttcatag cctgtcgtgc cgttgatttg cccggcgaaa
    25441 aacagtccct caatggtttt ggtttcgagg cttgctttga ggttgcgcgg atcgaagtag
    25501 tcgtattcga tggcgtagcc ggggcgcagg atatgggcgt tttcaagacc cttcatactg
    25561 cgcacgagcg cgatttggat gtcgaacggc aggctggtgg agataccgtt ggggtagtat
    25621 tcgtgcgtgg tcagaccttc gggttcgagg aaaatctggt ggctgtcttt gtcggcgaag
    25681 cggttgattt tgtcttcgat agacggacaa taacgcgggc ccacgccttc gattttgccg
    25741 gtaaacatcg ggctgcggtc gaagcctgag cggatgatgt cgtgggtttg cgtgttggta
    25801 tgcgtaatcc agcaggacac ttggcgcggg tgcatttcgg cgttgccgcg cacggacatg
    25861 acgggaacgg gcgtgtcgcc gggctgttcg gtaagttggg aaaagtcaat cgtgcgtccg
    25921 tcaatacgcg gcggcgtgcc ggttttcaga cggccttgcg gcagcttcaa ttcgcgcaaa
    25981 cgtccgccga gcgatttggc ggctgggtcg ccggcgcggc cgccttcgta gttttccaaa
    26041 ccgatgtgga ttttgccgga caagaacgtg cctgcggtca gcaccacggc gcgtgcttta
    26101 aactccacgc ccatcgcggt gatcacgccg ctgatgcgtt cgccctcgag tgttacgtct
    26161 tcgacggctt gttggaaaag gtcgaggttt tcttggtttt ccaacatttc gcggatggag
    26221 gctttataca ggatgcggtc tgcctgcgcg cgcgtggctc gtactgccgc gcctttgctg
    26281 gcgttcaggc ggcggaactg gatgccggat ttgtcggtcg ccaacgccat cgcgccgccc
    26341 aatgcgtcga gttcgcgtac caaatgccct ttgccgatgc cgccgataga ggggttgcac
    26401 gacatttgtc cgagcgtttc gatattgtgt gtgagcaaaa gcgtctgcgc gcccatacgg
    26461 gcggcggcaa gcgcggcttc cgtgccggcg tgtccgccgc cgacgacgat gacatcgtag
    26521 gttttggggt aaatcatgtg ggtcatgtgt gtattgccta tcggtttttc agacggcatt
    26581 ccaagcagga tgccgtctga aaaacgaaac ggattcaaaa gtaaagggtt gggattgtac
    26641 gcctgttcgc cctgttttta cagtgcgcgg aaagggaaaa gccgcttcgc ggggaagcgg
    26701 ctccggtaag ggcgggattt accaaacatc ggatttgatg cggcgtttca ggcccggatg
    26761 ttcggaaagt ttgaactcgg ggtctttgcc cattttcagc ttggcggtgt aatcgcgcag
    26821 cagcataaac gccaatggcg agagcagcag gatggcgacg aggttgatcc acgccatgat
    26881 gcccatcgcc atatccgcca tatcccagac caaaggcaca ttggcaaccg cgccgaaata
    26941 gacccacgcc aaaaccagca tacggaaaac ggcggtaatc agccaatggc ttttgatgaa
    27001 ttggacgttg gactcggcat aggcatagtt gccgataacg gtggaaaagg caaacataaa
    27061 caggatgacg gcgaggaaac ccgcgcccca ttgccccact tggttgacaa tcgccgcctg
    27121 cgtcagcgcc gcaccgctca aatcgccata aggctgttgg taaatcaaga tgatgaaggc
    27181 ggtgcaagaa caaacgatga tggtatcgac aaacacgccc agcatttgaa tcataccttg
    27241 cgaaacaggg tgtttcactt cggcggcggc ggcggcgttc ggcgcggaac ccatacccgc
    27301 ctcgttggaa tacaggccgc gtttgatgcc catcatcatc gtttgcgaaa tcagaccgcc
    27361 gagtaagccg cctgctgccg cgtcgaattt gaacgcgccc gaaaaaatct gaccgaacac
    27421 gtccggaatc atcggaatat tggtcaaaat gatgaaaagc gcgataaaga ggtacaaaac
    27481 cgccatcagg ggcacgacga tttccgccgc tttggatatg cgcctgatgc cgccgaagat
    27541 aatcggcgcg gttaaaatca ccagggcgac gccgacataa tgaggctccc agccccacgc
    27601 cgctttgacg gtatcggcga tggtattggt ctgaaccgct tcaaacacaa aaccgaaaca
    27661 gaaaatcagg ctcagggcga acaacacgcc cagccatttc tgccccagac cgtgcgtgat
    27721 gtagtaggca gggccgcccc ggaaatggtg gttgtcgcag tcgcggactt taaagagctg
    27781 cgccagcgaa gattcgacaa acgccgaact catgccgatt aaggcggtta cccacatcca
    27841 aaacaccgcg cccggcccgc cgactttgat ggcgattgcc acgcccgcga tattgcccac
    27901 gcccacgcgg ctggcaaggc cggttacaaa tgcctgaaac ggcgtgatgc cgtgagggtc
    27961 gtccccctgt ttgcggccgc cgagcatttc tttgatgctg cgcccgaaca ggcggaattg
    28021 gacaaagccc gtggttacgg tgaagaaaag ccccgtaccc aaaagcatat aaaccaagta
    28081 tgaccacatc ggatcgttga tggcgccgac ccagccgtgc agccattcgg taaagttctc
    28141 gttcatatcg cttccttaaa gttgaaaccc gcacatatgg cggtatgcaa gcagggttta
    28201 aattttgtaa acgcccattc tagcagattg tcaacaaaat cagaaaaatt tacatcgccg
    28261 cgcgcctgtg gcgttagaat cgcgttttgt ttggagcaaa cacgatgaaa cagcctgttt
    28321 ttgccgttac ttccggcgag cctgccggca tcggccccga tatttgtttg gacttggcgt
    28381 ttgcacgcct gccctgccgc tgcgcggtat tgggcgacaa acacctgttg cgcgcgcgcg
    28441 ccgaagcctt gggcaaaagc gtcgtcctgc gcgacttcga tccggaatca ggcggcgcgc
    28501 acggagaagg cgggctggaa gtgctgcaca tccctgccgc cgaagcggtt gaggcagggt
    28561 ggctcaatcc cgccaacgcc gcctatgtgc tgcaactttt ggacaccgcg ctcgcaggca
    28621 tttcagacgg cattttcgac ggcatcgtta ccgcgccgct gcacaaaggc atcatcaacg
    28681 ccgcgcgcgc aagcacaggt tttttcagcg gacacaccga atatctggcg gaaaaaagcg
    28741 gcacggggca ggtcgtgatg atgcttgccg gcaaaggcct gcgcgtcgcc ctcgtaacga
    28801 cccacctgcc gctgaaagac gttgccgccg ccatcacgca accgctgatt gaaagcgtcg
    28861 cacgcatttt gcatcacgac ttaaaacaca aattcggcat caaaaatccc aaaatcctag
    28921 tcgccgggct caatccccac gccggcgaag gcggacacct cggacacgaa gaaaccgaca
    28981 ccattatccc cgcgctcgaa aacctgcgcc gcgaagggat aaacctcgcc ggcccgtatc
    29041 cggcggacac attgttccaa ccgtttatgc tcgaaggtgc ggatgccgta ttggcgatgt
    29101 accacgacca agggctgccc gtgttgaaat accacagctt cgggcagggc gtgaacatca
    29161 cgctggggct gccctttatc cgcacctccg tcgatcacgg caccgcgctc gatttggcgg
    29221 caaccggcag ggcggactcc ggcagcctga taactgccgt ggagaccgcc gtcgagatgg
    29281 cgcgcggcag cctttaaaga tgataaaaga cccgtcattc ccgcgcaggc gggaatccgg
    29341 accttcgggc ggcggcaata ttcaaaggtt atccgaaagt ttgaggttct agattcccgt
    29401 tttcacggga atgacgaaag gtggcgggaa tcacgggaat ggcgaagttt caggcggtat
    29461 tgcaggtatc cgaaaccatg caaaaagagg ttctgcggaa cagaacctct ttttgccgcc
    29521 gtcggttcag ccttgccggg tttcgacttg gatcatttct tcggcaggga cggttgcggc
    29581 ttcagacggt tttggctgtt cggaacggcg caaaccgcgt ccggcttgga cttcgggttg
    29641 tgccgcccat gccttcaatg cggcagggtc ggtttcgatc aggacgagtc cgccggtttg
    29701 tgcggtttcc cgtgcctgtt ccgccgcagc cgtaaaggtt gcggtttcag acggcatttc
    29761 ctgtgctgcg gctttcggtg ccgcgccttc gggcaggatg tcggcggcgg cacggcggat
    29821 tttttccgcc gcatcataaa tcagtgcgtc gccgtttgaa acggcggaag atgccgtctg
    29881 aacgggtgtg gcgatgagcg gatcggcaat gctgacggta atcggcgcgt ttgcgtcggt
    29941 ttcgccgaaa acgtgcgcgg cggcggaacg gactttgtcg gcggtgtcgt gaatattcag
    30001 gtactgctcg attttggcgg cagacgggat attgcgtttt ttgccgtttt gacggcggtc
    30061 gcgctgattg ttgcgttcgc ggcgttcttt ggcatcgcgg ctgtcgcgtt cgcggcggtt
    30121 gcgttcggat ttgggcttgc tgcctttgtc ttctgcggta tacggttcgg gcggcgtgtt
    30181 ttccgctgtc tgaacggctg tttcggcaac ggcggcggtt tccggtgcgg tttggccacg
    30241 ttcgttgcgg ctgccgttgc ggcggcgttt tccggtttga ccttcggttt cgggcggtgc
    30301 ggcatctgca acgggtgcgg caggctgtac gttgcggctt tggatttccg cttcgttggc
    30361 gcgttcggtg gcacggtcgc cgcgttcgtt gcggcggcgg ttgccgttgt tgcgcgtttc
    30421 ggctttgtcg gcgcgcgctt tctgtccggc agttttgccc gccgcttcgc ggacttctat
    30481 tttgctgcct tcgcgtttgc tgcggcgcgg gttttggcgg cggttgttgg cgcggctgcc
    30541 gctgcggttt gccgtgctgc gtttttcgga ggtttcggca gcgggtacgg cttgggtttc
    30601 gctgccgccg aaaatgcgtt tgagccatgc tttgaagctg tcccaccagg aggttttttt
    30661 ctcgggggcg gcagtcgggg cggggctggt gtggcgcacg cctttgacgg cgggttcggg
    30721 gcg
//
LOCUS       DAXFWM010000018        29110 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000018 DAXFWM010000000
VERSION     DAXFWM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.018"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            156..1145
                     /locus_tag="ACFI8B_000788"
     CDS             156..1145
                     /locus_tag="ACFI8B_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3834055.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            1487..1966
                     /locus_tag="ACFI8B_000789"
     CDS             1487..1966
                     /locus_tag="ACFI8B_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC3834056.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(2026..5073)
                     /locus_tag="ACFI8B_000790"
     CDS             complement(2026..5073)
                     /locus_tag="ACFI8B_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFC3834057.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTSASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISEAAPILLDTIAEYMGKSRAEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLA
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYQIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGKNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVQESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(5103..5252)
                     /locus_tag="ACFI8B_000791"
     CDS             complement(5103..5252)
                     /locus_tag="ACFI8B_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834058.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            complement(5564..5737)
                     /locus_tag="ACFI8B_000792"
     CDS             complement(5564..5737)
                     /locus_tag="ACFI8B_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834059.1"
                     /translation="MTTAVKPPADLVRPCPKLPHLEGNTGADVLPWSLQVIGLYKDCK
                     ARHGALVRALGAD"
     gene            complement(5721..6065)
                     /locus_tag="ACFI8B_000793"
     CDS             complement(5721..6065)
                     /locus_tag="ACFI8B_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834060.1"
                     /translation="MIGALLKNWKPLLILSAIAFFAVSWQLDRAAQYRRGYGAAVSEV
                     SERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADG
                     VRELNAAVDDGG"
     gene            complement(6066..6605)
                     /locus_tag="ACFI8B_000794"
     CDS             complement(6066..6605)
                     /locus_tag="ACFI8B_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC3834061.1"
                     /translation="MKELKWIEEARKYLGAHEKVNGKSNPVLLAMLQEMGNFNQEQKA
                     WWKETDTPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKAVPSSPAE
                     GRYRLSDVAATAKQGAGEA"
     gene            complement(6647..6772)
                     /locus_tag="ACFI8B_000795"
     CDS             complement(6647..6772)
                     /locus_tag="ACFI8B_000795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834062.1"
                     /translation="MPPETDFAKRRRVTGVEMETERVKPDVSVLRKPAIKGLAGV"
     gene            6812..7048
                     /locus_tag="ACFI8B_000796"
     CDS             6812..7048
                     /locus_tag="ACFI8B_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC3834063.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            7048..7395
                     /locus_tag="ACFI8B_000797"
     CDS             7048..7395
                     /locus_tag="ACFI8B_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC3834064.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(7450..7590)
                     /locus_tag="ACFI8B_000798"
     CDS             complement(7450..7590)
                     /locus_tag="ACFI8B_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834065.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGVAVYWRPPEILSA
                     LK"
     gene            complement(7751..8083)
                     /locus_tag="ACFI8B_000799"
     CDS             complement(7751..8083)
                     /locus_tag="ACFI8B_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834066.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(8084..8554)
                     /locus_tag="ACFI8B_000800"
     CDS             complement(8084..8554)
                     /locus_tag="ACFI8B_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834067.1"
                     /translation="MLRVLLNRRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(8625..8930)
                     /locus_tag="ACFI8B_000801"
     CDS             complement(8625..8930)
                     /locus_tag="ACFI8B_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834068.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(9042..13187)
                     /locus_tag="ACFI8B_000802"
     CDS             complement(9042..13187)
                     /locus_tag="ACFI8B_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC3834069.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLIW
                     YGDFVTIEHKAVTRQGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLKQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTEQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQIYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            13427..13705
                     /locus_tag="ACFI8B_000803"
     CDS             13427..13705
                     /locus_tag="ACFI8B_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC3834070.1"
                     /translation="MESQTFASVFDALCDTPAEAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(13733..14164)
                     /locus_tag="ACFI8B_000804"
     CDS             complement(13733..14164)
                     /locus_tag="ACFI8B_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFC3834071.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(14166..15020)
                     /locus_tag="ACFI8B_000805"
     CDS             complement(14166..15020)
                     /locus_tag="ACFI8B_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFC3834072.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVRIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(15017..15616)
                     /locus_tag="ACFI8B_000806"
     CDS             complement(15017..15616)
                     /locus_tag="ACFI8B_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFC3834073.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(15616..15882)
                     /locus_tag="ACFI8B_000807"
     CDS             complement(15616..15882)
                     /locus_tag="ACFI8B_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834074.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGNELSEDEFDALLKAKGII"
     gene            complement(15894..16223)
                     /locus_tag="ACFI8B_000808"
     CDS             complement(15894..16223)
                     /locus_tag="ACFI8B_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834075.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRKEQEAAQA
                     GEARAAD"
     gene            complement(16284..17057)
                     /locus_tag="ACFI8B_000809"
     CDS             complement(16284..17057)
                     /locus_tag="ACFI8B_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834076.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDDDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(17083..17511)
                     /locus_tag="ACFI8B_000810"
     CDS             complement(17083..17511)
                     /locus_tag="ACFI8B_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834077.1"
                     /translation="MNREAVYSALWAKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(17508..17990)
                     /locus_tag="ACFI8B_000811"
     CDS             complement(17508..17990)
                     /locus_tag="ACFI8B_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFC3834078.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQSEVVL
                     NRLSGRVLGVRTGDLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(17990..18520)
                     /locus_tag="ACFI8B_000812"
     CDS             complement(17990..18520)
                     /locus_tag="ACFI8B_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFC3834079.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(18523..18885)
                     /locus_tag="ACFI8B_000813"
     CDS             complement(18523..18885)
                     /locus_tag="ACFI8B_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834080.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     AAEPAEAEAEPAEAEKAE"
     gene            complement(18892..20391)
                     /locus_tag="ACFI8B_000814"
     CDS             complement(18892..20391)
                     /locus_tag="ACFI8B_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834081.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKLGVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(20432..21589)
                     /locus_tag="ACFI8B_000815"
     CDS             complement(20432..21589)
                     /locus_tag="ACFI8B_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC3834082.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVELSESEIAALKAVLAKA
                     DKPKGGPAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
     gene            complement(21657..23804)
                     /locus_tag="ACFI8B_000816"
     CDS             complement(21657..23804)
                     /locus_tag="ACFI8B_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFC3834083.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVAGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPGHPNCRCAVVPVLAGDMPES"
     gene            complement(23801..25222)
                     /gene="terL"
                     /locus_tag="ACFI8B_000817"
     CDS             complement(23801..25222)
                     /gene="terL"
                     /locus_tag="ACFI8B_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC3834084.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(25284..25733)
                     /locus_tag="ACFI8B_000818"
     CDS             complement(25284..25733)
                     /locus_tag="ACFI8B_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834085.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKSELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(26026..26895)
                     /locus_tag="ACFI8B_000819"
     CDS             complement(26026..26895)
                     /locus_tag="ACFI8B_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3834086.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRRAVAALVGRKRIDYSSAYSMIHQRFNVESV
                     EGIPAGKLPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPISGNALYDLAVAVSYG
                     ARAIQMGRDVSLPLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKI
                     RPILPEIRNLSAV"
     gene            complement(27177..28337)
                     /locus_tag="ACFI8B_000820"
     CDS             complement(27177..28337)
                     /locus_tag="ACFI8B_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC3834087.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVVWLVIDSYRFCCDDENFKRKPK"
     gene            complement(28428..28835)
                     /locus_tag="ACFI8B_000821"
     CDS             complement(28428..28835)
                     /locus_tag="ACFI8B_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834088.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
BASE COUNT         6217 a         8915 c         7286 g         6692 t
ORIGIN      
        1 gcatctacgc ttattctacg tccgcacttc tttcgtcttt ttaaatctat ttatccctac
       61 atatcaaagc attaaattta atatagccct attttacccc gctttatatc tgttttgctc
      121 tattatattt gcacccgaat tgcacccgaa taaatatggc aaccatcact caacgtaacg
      181 gcaaatggcg cgtacaaatc cgtatgaaag gcgtttcccg ttccgccaca tttgagcggg
      241 catcagacgc gaaggcgtgg gcggcgcgaa tagagtcgca aattatggac ggcatccaag
      301 gcaatgcccc gcgaaatact atctttgccg accttatccg gcggtattta tccgaggtta
      361 caccatcaaa gcggggcgca cgggaagaat cataccgcat tggacgcgca ctaaaaacac
      421 ctttggcaaa ggtgcggctt gccgacctgc gccctcaaga cttcgccgat tggcgagatc
      481 aacggttgca ggaggtatcc cccaccagcg tcggacgcga attaaccact ttatccgccg
      541 tctgcgaaca cgccatgaaa gagtggggac ttcttcgcga aaaccctgta cgcaaaatca
      601 gcaagccgaa aaaaagccgg gcaaggacaa gacggccaac cgagcaggaa attgccgata
      661 tttgcgccgc cctcctatac cggcctaatg agaaaccgaa aatggcggtg caacgggttg
      721 ccgttgccgt cctatttgcc atcgaaaccg ccatgcgggc aggcgaaatc tgcggcctaa
      781 aatgggcaga tgtgaatatg cggcgtcgta ttgcccacct cccaataact aaaaacggcg
      841 acagccgtga cgtgccatta tccttgcgag ccgccgaact aatagagcaa ctgcgcggaa
      901 ttgatgacac atgggtgttt agcctggatg ccaaaagcct tgatgtatta ttcaggcgag
      961 cgcgggataa ttgcggcata caaggcctgc attttcacga cacccgccgc gaagccctca
     1021 cgcgcctatc aaaaaaagtg cctgtggagg tcttggcaaa aatcagcggg catcgggatt
     1081 taaggatttt actcaatgtt tactaccgcc ccgacatggc ggatattgca aaaatgttgg
     1141 attaaaatat ttttatttaa aaacataggg cacactgcgt attgaaaata aagaggaaaa
     1201 ttaattatct tcggtttcaa aggttcatta aaaaagaatc tataaaaaat gccgtccgaa
     1261 agcccttcgg acggcatttt tattgaaagg ctctcttcaa ccgctttaca cgaaggcggt
     1321 ttttttgtat agtccggtcc gcggggtgcg gaatcttgaa aatagtcagg caatgccgta
     1381 tattccgacg caaggattta ttctcaacat cagcttaagg ggatgacaat ggggcatatt
     1441 tattcggaaa gccgtgattt ccgcctttcg gacggctggg acggcaatga ctgacaacct
     1501 aatacagata gcaacgccga tattgactgt tatcggcgtt tttgttgccg cttacggcat
     1561 catgaggaat acggaaaacg ccaaaaagcg cgccaccatc gacatgatca tggccgaacg
     1621 taacaatgcc gcccttcaag aagccataac catagtaaac gggctggcaa aaacagacgg
     1681 atgcatactc gccacctata catcagatac cccggacaag aagaaagacc gtgaagccat
     1741 actgacagtt ttaaaccagc gcgaatttgt ctgtgcgggc gtattgggcg gggcgctgca
     1801 cgagaaaatg tataaagatt tcgaatactc catgctgtta cgcgactggg acaacctaag
     1861 cagcttcatt tttgaaatac gccgtatcag gagcgcgccg acggcctttc aagaatttga
     1921 agccgtagcc cggaaatgga agaaaaagcc tctgaaaacc aaatagctta atagcttgac
     1981 gtacgccgca acataggccg tccgaacctt tcggacggca ttttctcaga caaatttaaa
     2041 gtcccgtttc atctgcttca agagcttcgc caagtcattt ttatggatga agtcgccgcc
     2101 tgtcgaattg atgatgacgg tgctgccgcc tccctgctga tccgccattt cacggatggt
     2161 ttgcgcgtgt tcggcaggca ggaccatctc gttttcgtgc agttgggtca gcgggttgat
     2221 gcctgccggg atgtcccagc cgcctgccgc cgagggaatg cgcgtcgtgg ttgtggtcgt
     2281 ttgagaaccg ccgccgccgc ccatcagtcc cattacagcc gacatcatcg ccgccatagc
     2341 cgcaacggca agaatcgggc cgacatatgg gatatgcgcc tgggaagccg ccgcgcctga
     2401 agccgcttga acagcattac tactgacaac cgccgttgtc tcggttgcct tagttgccgc
     2461 cgtcttggct gccgccgctg tctccagcgt ttccttagtt ccgaaaatca tcttgtaaat
     2521 ggccgattcc tgaaccatgc gccgcatcaa gcccgtcaaa ggtttcgtaa ccatttcttg
     2581 aataaaggtt tgccccatac tcttgaaaaa gccgttcatc gcctgcctga aattctgcgc
     2641 cctcgccagc atggcggtga acgcctgccc catctgctgc tgtgcttcct gccaaacgtt
     2701 cttgccgccg tcttgcagca tttccatgac gttgggcgcg tctttgcggc gttggttttc
     2761 gcgtttgccc tcgttcttcg cctgcgtccg ttcgtgcccc cgccccaatt cgcccatttg
     2821 ggccttgagc ttgccgattg ccgcctgact gtatgccggg tcttgttcgg caagtgcgat
     2881 gcgttcctgc aatgcgtcat aggcgatttg gtaacggcgg ttttcaaact cgatttccaa
     2941 gtccaggcgt tcgagttgcg aaatacgtcc gtctgccagg gcttggtctg ccgcgtcttt
     3001 ctccatctcc agcttgtgct tgtccaattt ctcccattcg gccgcctgat tcattttggc
     3061 ttcggttgac tgcttcgata attggtcttc aagcgtcagg attttttcgc ggatttttac
     3121 gcctgttttt ccgttggcgt ccaccgttgc cagcttcgcc cgccaatatt ccgcctcacg
     3181 cgccaaatcc cattcttggt gtgcgagcgt ttcgcgctgc atttcgcggt gtgcgagttt
     3241 tcgggctttg atttcccctt cccaagcctg catcgggtct ttggccgcgc ccggaccgcc
     3301 cgaacgggat ttgccccctt tgccgccgcc ctgatttgcg cccggctttt tccgggcggt
     3361 tttggcgtgt cctccgccgc cgcgtccctt gagggcatcg gcttcgtgaa tatcggcggc
     3421 ccgttcgcgg acggcatttg ccattgcgcc ggcgcggtct ttcgtcaagc tgtcatgaat
     3481 acgctcgcca agcccgccgt cgtccatccg ccccatccga accttgttca gctttccaat
     3541 gcccgatatc ccggccatcg acgcggcttt gttggcaaag tcaatcatgc tgttaatcat
     3601 gcctaccgct ttgtttatca tccgttcgat cgcggaaata aacacgttgc ctatggcctt
     3661 gccgagattg gcgaagaatt gcggcatatc gtttgcggct tctttaatca gcatccagcc
     3721 ggttgcgaat atgttgatat aaacgttcac atacgcgccg attgcgcttg agatgacgct
     3781 catcacgcgc ccgaacaacg acggccagcc gcccgcgccc tcgttcagcc ggcctgtcag
     3841 cccgtcaaac cacgatttga cggttcccac cgcttccccg acggtttccg tgatgatttg
     3901 ccaaaccgcc cgaatcacat cggaaagatt cgaccagccg ccgccgaaaa catcgatttc
     3961 atcgccgaat ttcgcaatca gcccgacaac cgtgccgatt gcgacggcaa taatcccgaa
     4021 cgggtttgcc agcagtgcaa gattcaaagc cagtgtcggt gcaacggcgg cggcaacggc
     4081 aacggcaaaa cctgcgacga tagggacgac caaattcagg ttatcggcaa tcagtttaat
     4141 aacggcggca atccccgaca ttgtgccgct gtcgttcagc agttttgaaa ccatgctttg
     4201 ccagttgttc gagaacaccg tcaaagcctg ccccatcgtc acgggcattt tggccgcctg
     4261 ctccccgaac ttctccgacg cgccggaaat ggctttgaaa atcacatccg ccgtcaattt
     4321 cccttcgctg cccagctttt tgatttcagc gcgggatttg cccatatatt ccgcaatggt
     4381 atccagcaaa ataggtgcgg cttcggaaat ggatttaaat tcatcgccct gcaatacgcc
     4441 gctgcccaaa gcctgcgata actggaacag cgcggcggcc tgttgtcgcg cgccaacgcc
     4501 gccgatggtc atcgcgttgt tggttgcctc ggtgaatttt aaaatttcct gctgcgtgta
     4561 gccgtagtct ttcaacgcgc ggcttgtgga aacgtacagg tttgccgttg attcaagcga
     4621 tgcactggta cggtttgccg tatcgagaag ctgctgctgc acggccagat actccgtttc
     4681 agacgacgtg acttgtcgga cttggctgtt tatcgactgc atcgcatcgg cggtatcaag
     4741 caccgatttg gcgaagtctg cggttgctaa tcctgccagc agtttggtca gccccgataa
     4801 gcctgagccg attttaccca cttcggccct aaccttttca gacgcctgtt gcgcctgatt
     4861 gcccgccgct tcaaatcccg ctttcacatc ggcgtgcaaa ttgcgcatgg tctcgtgcat
     4921 actgtcgatt gcagctttaa agccctttgc ggcattatcg ccgctgctgc gcatcttttc
     4981 cgccgcctta tcagcatcgg cggcggccga atcaaaaccc tgcttcgtat ggttttctac
     5041 cgttatcctg actttggctt ccaaatcgct catttacagc cccaaataaa aaagccgtct
     5101 tttcagacgg cataacgttt caacatttcc cgataattcg ccaattcccg tccggcaaac
     5161 tcaaaggcct tcaaatcggc acgatcgccc gcatttcggc gcccgtccgg ccggcaggcg
     5221 gtttgttttt cacaaaatcc ttcggcgttc atctctgcac cctgtcaaaa taaaaggctt
     5281 ttttgcaacc gctttacacg aaggcggttt gacgcgcagg gaatacaaat gccgcctgaa
     5341 cctttcagag tgcgaagcct gcccataggg tacggcattt tcatttccgc ccattattgg
     5401 tgcgcctgat aattttgtat aacgccccct tcgcgcaaac aaaacggcaa aaatttgccg
     5461 cgtccgcccg atatgtaaaa aatcgggggg aatgccgacg cttcccccga tgcggccgtc
     5521 aaccgaaagt tgaccgttcg gttgcaaact tccggttgac aactcaatcc gcgcccaacg
     5581 cccgtaccag cgcgccgtgc cgcgccttgc agtccttgta caagccgatg acttgcaacg
     5641 accacggcag cacgtccgcg cccgtgttcc cttcaaggtg cggcagtttc gggcagggcc
     5701 gcaccaaatc ggcgggcggc ttaaccgccg tcgtcaacgg cggcgttgag ttcgcgcacg
     5761 ccgtcagcat caaaacaggc attgcggtac acaggttttt caatgatttt aagcgtttgc
     5821 acatagcgca ccctttcttt ttcctcgcgc gccgcctttt gcgcctgata cgcggcggac
     5881 gatttgcggg cgtgttcggc gtgttcgacc gcggcggctt tgaggcgttc cgaaacttcc
     5941 gacaccgccg caccgtatcc gcgacggtat tgcgccgccc tgtccagctg ccaagaaacg
     6001 gcgaagaacg cgattgcgga caaaataagc agcggcttcc aatttttcag caaagcccca
     6061 atcatttacg cctcgcccgc gccctgtttc gccgtggcgg caacgtccga caaccggtaa
     6121 cgcccttcgg cgggggacga aggcacggct ttgccgccaa tcagcttgga cggccagaag
     6181 tagccgtcaa tgtccgcagg gtcaaacggg atgatggata ccatattgcc ctgattgccg
     6241 cccaagccca agattctgcc ttccgcgtct ttgccgacaa cgaagaacac gtgtccgccg
     6301 ccccggcgcg gtttgaccgc gatgcagccg tatgcggggg cttcgagttt cgtcaaaccc
     6361 gacattgacc aggctttggc gcgataccag tccctgatga ccgcgcgtcc cgctttgccc
     6421 aggcagtgtc cgacaaacag gccgcaccac ggggtatctg tttctttcca ccacgctttc
     6481 tgctcctgat taaaatttcc catttcttgc agcatcgcta aaagcacagg gtttgacttg
     6541 ccattcactt tttcgtgagc gccaaggtat ttacgggctt cttcaatcca tttcaattct
     6601 ttcatttctt ttccttccga taaaagacga gatgtaaact ttcggtttac accccggcca
     6661 agcctttgat tgcaggtttc cgcaacaccg atacatccgg tttcacacgt tccgtttcca
     6721 tctccactcc tgttacacgc cgccgcttgg caaaatccgt ttcaggcggc ataatgtatc
     6781 gacaacgtat ctacaacacg aaagcaaaac catgttgcga ctgcaaaaat ggggcaacag
     6841 cgcggcagtc cgtctgcccg cccgcctcct gaccgccctc aacgccaaaa tcggcgacgc
     6901 gctggaaaca gaaatccgca acggcgagct gctcatccgc cccgtcaaac gctaccgcct
     6961 gtccgacctt ttggcggaaa cggaagccgc cccgccgcgc gcggaaggct gggaagaaat
     7021 gcccgatgtc ggacaggagg gcgtttgatg tatattcccg agcgaggcga cattttccat
     7081 cttgccttcg accccgccgc cggtacggaa atgaaaggcg gccattacgc catagccctg
     7141 tcgcccagag cttacaaccg cgcaaccggc ttggtttacg cctgcccgat ttcacaaggc
     7201 agggcggcgg cggcaagaag cggcggtatg atttccacac tgctcggcgc gggaacggca
     7261 acacaaggca atgtccactg ccaccggatg aaggcattgg attggaaaat ccgccgcgcc
     7321 gccttccgcg aaaccgtccc cgattacgtg attgaagacg tattggcacg catcggcgca
     7381 gtactgttcg actgacttca gagtgcgaag cctgtccgca gggggaagca tccgtttcca
     7441 tacccgcccc tatttcaaag ccgacaggat ttcaggcggc cgccaataaa ccgccacgcc
     7501 caaaaccgca taaaacatac ggcgcaacac cgcatactcg gcaaacagct tcaacatctt
     7561 gcgaaactcc ctgtttatgc taaacttcaa acagatttct ccttacctcg gataaggtta
     7621 aattcagaaa cgccgcaagg attccgcccc tcgcggcgtt tcgccgtttt caaacctgcc
     7681 gcgtaatgtc gtgcgtcagc cggacgcgcc cggaacacag cgtcttcacg cgccgcgata
     7741 ccgctcgaaa tcaatctttt ccatccgaag accctccgtc tttcacaccc gtaagctttt
     7801 ccgccaagcc gcccagataa ccgcgcaaaa ccttgggcgc gagcgcgcgc accgtatcga
     7861 gcgcgtgccc cgtcatcatc cccgcgaaca cccccgccgc cgcgcacgtc cacgcctgat
     7921 ccagcggcac gaaccgctcc gccgcggccg ccgccgcgat tgcagaaata aaagcctcca
     7981 gcacagcctg cacggggcgg ccgcgttcct gaaggcttgc ccacaccgcg cccagcacgc
     8041 cgccggccgc ggcaaacagc agtccgaact gcaaaaaccc cattcacgac ccctcatccg
     8101 ccaaaccccg cgacttttgc gccgaaaaca aaaacctcaa cgcattattc cccgccagcg
     8161 cgcagaaaaa cgccaacagc ggcggaacga ccatacccgt gttcaaaggc gggtacgccc
     8221 cccaaaacgc caccgaaacc aaaaaccaca caaagcccga aaacagcaaa aggtaggccg
     8281 aagccacaca agccctcacg ccccgcgcaa acagcaacgc cgtctgaagc agccccgccg
     8341 ccgccagcac ccccaccgtc cacgcctccg gaatatcctg gaatttgtaa taaatccgcc
     8401 acgcgtaaat ctcgtcgggc gcaagcgcga acaccgccgc gtaacccgac aagcccaaac
     8461 cgctgaccgc ctccagcacc cgcgtcgccg tgccgaacaa ccacgcctga aacccggcgg
     8521 gcaaaaaccg ccgattcaac aaaaccctca acatacccgt cacacacccc ccggcaaaac
     8581 accgcgaagc cgaagccccg cagtgtccga cgccgtctga aaacttattc cgcgtaaacc
     8641 ggttgcgcgt ctttggggat gcccgccgca ccttccaacg ccttgcggta cacccaaccg
     8701 gggtcgggcg cggttatgtc cgtttcctga aaatccaatg tgcgcgccaa caggtagcgt
     8761 ttgcccgatt taaacgcatt ttccgacaca tagccgttca acgtcgccgt aacataaccg
     8821 ttgcggtaat ccgcgccgat atgttcgacg acgtggtagg ccgcactcgc gccggtcata
     8881 ccgtcctcga ctgcaaaatt caaggcaaca atctttttat cttcaatcat ttcaaatctc
     8941 caaaagcccg ctaggggcgg taacgaaaat gccgtccgag gtcttcagag ggcattgccc
     9001 ggacaaaaca ggaaaaaccg cagagccggc atacgggggc gtcagccaac ctgccaaacc
     9061 tcgttttcgc gcgattccgg aatcagcacc ctgatattgc gcagctcgat atactgccac
     9121 cagtgttcca caaaaaacag caggttggat ttcggaacgg cagtattaaa ctcctcctga
     9181 aacagagaat actgttgggg cgcatgaccc cggtatttct taaccactac aaacttatcc
     9241 gacctgtcga acgcaagcat cgtactccag tcggcattgg taaaaggccg gtactcgaac
     9301 gatatgccgt agatatcgtc cggcaactga agcagcccgc cgtaagcatt ccaatgttta
     9361 tacatctgat tttggtaatc gtgtacacgc cggtcgccgc ccaaataaat ctgcttctta
     9421 ctgtcgtacc ggaaactttc cgcaaaggtg cgccaaacca tcctcccgag cagctgcttg
     9481 tattcgtagt cggattgcgc caaatacgaa accgacacga acggcgaaaa caccgattcg
     9541 tgggaagcga gcgacaactt caggctgacg ggtttgcccg cggggatgac ttgaatctgg
     9601 tactcgaggc gggttctgta ccttgagccg gattgttcgg tataatcccc gccccccctt
     9661 gcccgggggg gcaggtcttc gtacctcccg aaagttcggc cgcgccaaat ataattggta
     9721 tagttctcgg tttccctgac ttccctcggc gcaagcacac ccccgttcaa cagcagcttt
     9781 gcctgcaccc tgttgttgcc gaacgtttcc gaaaccacat aaaaattcgg aatcaaaacc
     9841 ctcggcagcg ggtctttatc caaatccaac acccaaacgt taccctccgt ccaacgcagc
     9901 ctgtgcgccc tcaacacgtc gccctcgacg gacgaagccc tgaccgtacc gttgaaataa
     9961 cccgaatcgg cctcaatccg gccgcgtatt accgcattgt cggccgtcag gatgccgtct
    10021 gaagacaccg taaaattacc cccgccgata ttcaggctgc cgcccgtgat gcttccgaga
    10081 ttggaagata ccgcgctcaa atcggcaacc tgaatcttat cggcagtcac cgcgtttgcc
    10141 ccgattttat cggcggtaac ggcggcagcc ccgatttccc gcgccgtaac gcttccggct
    10201 ttcaggcggt ttgcgtccag cgtgtccgcc gcgattttgc cgccgtggat atccccggcg
    10261 ttcaacttat cgacaaccgc cttgccgttg accaccaatt cgccgttgat gcccacgcgg
    10321 tttttctgcg tgtccaccac gaacgggaac acatccgcct tcccggccgc accgatgccg
    10381 aagcggtcgg cattcacgat aaacttgctt tcgggcgtgc cgtttttcgg cgtagtcgcc
    10441 aagccgaagc ccgccacttt gccgtttgca tcaaccttga ccgtgtattg cgcctccaag
    10501 ccgttgatgc ttcgggcgtg tgtctgaacc gccgccgcat tgccgtccgc cgtcgatcgg
    10561 actgcctcga cgcgctcgtt gacgctgccc gcgccgttgc cgtcaatcag gttgattttg
    10621 tcgcgcaaag ccttgttcag actgctttcc gacaggtcgg ttgccgatac gtcgtaaacg
    10681 gtaaacgata tgctgccgct gattttcagg ttatctttgc cgaaactgtc gtaacccgcc
    10741 gccctcaagt aataggtctt tcctttctcc agcggtttgc cgccgcattt ggcgatggtt
    10801 ataaacgttt ccgcgccgtc ataggctttg tttgcgtccg cggccgggca ggctgcgttt
    10861 tcgcaaaccc aaacgataat ccctgcaaag tcttcttcgt caggtttttg gcaggtaaaa
    10921 aacgcctgtt tcaaaccgct gtcgatggaa ataccttgta atgcctgcag ttgcggattt
    10981 tgcgccgcga tttgcgccca attgcccgtt ttaccggtaa cggcacgccc gcgaaccttg
    11041 aagacaatac cgcgtacctg gccgccgtcc gccttcatat ccgcctgagt gtaggtgtag
    11101 ctgttgtcaa cgatgccgtc aaccgcacgc agacggcgtc ggctgccgcc tgcgtagatt
    11161 tccacgtcgt aggtatccgc gccgtccaat ttatcccaag cgatgacggc ttctttgccg
    11221 tatgcccacg atgatgtcag gcgcaggttt tggatttgac cgagcggcgc gccttcgatg
    11281 gtgtaggaat acgccggcac ttccgccaaa tcctgaatgc cgccgccgaa aacgttgtac
    11341 gaaaccagtt tgacccaaac cgtgcggcca atccagttgc gcggcacggc gtatttgaac
    11401 aatgcttcgt cgatgcgcgc aaaccggctg cccgccgcgt gtgcgttgac ggcggagccg
    11461 tacgcgccgc gcgtcaggtt gcccaatgtg tagcgtccca cgcccttcag ttcggcgtcg
    11521 gcgtatgcca gaaattcgcc gtcgacgtag cacaatgtca gcaaatcgcg gctgtcctgc
    11581 tccgtgccgc ccgtcagctg ccccgccgaa atttccacgc ccaacgtatt tgtgcggtcg
    11641 aaaaccgcgc cgtcgggcaa atcggcggtc agcgcgccga aacgcgcctt gcggttgacc
    11701 gcgccgacgc gggtgtagct gtcgccgtcc gccgataccc acacttcggc gccgccccac
    11761 ataccgcccc cggcggttgc cagccagatt tgcggttcgc cgcccgtcag ttgcaacggc
    11821 gcttcgaaaa ttaccggcac atgggcgttg cccggcgaaa cgttgtagtc ggccgaataa
    11881 cccgaagacg gctgcgtcgg atattccgac acggtataaa caccggccgg gcagtcttcg
    11941 gccttgacgg ataaaacccc ttctccgtct tcttcgattt ccgtgattcg gacgggcgtt
    12001 ttattcaggc cgagccccgc gtcggtcagg gttacgatgt ccatcggctc aagcaggcag
    12061 tatttccaac ccagcttaaa ctcatattcg ttgcgcacgt acagggcgcg ttgcagcagt
    12121 tgttgcgcca ctttttgcgc caccttcgca tcgcaaatgc cgtgcatcct gaccgcgtct
    12181 ttcgggcgca atccgtactg ctcgatattc gcctggtctt tcgcttcggc gatggcgacg
    12241 ttgtagtcgt tgtccctgtc aaggtactcg acctgaatct gattaaacgc atcggcattg
    12301 gttttgcgcc cggcctttac agggtcttgc gcgcccgaaa cgatgaaatc gtcatcggtc
    12361 aggtcgtagg cggccttatt gtcggcaacg tacaccgcgc cgttccccga atggctgccg
    12421 tccccgtagg ggatgatttt caggcggcct tgcgaaaata ccgccgcgct gttggtctgc
    12481 tccagcagtt cggaaatatt ccgttgcgcc tccccctgtt ccgtgtaggc agggcttagg
    12541 aaaatcccga cggcgcggca ataattgctg taccggtcgg tatcgccgat gttttgggac
    12601 gggaaaccgc agccgtagcg ttggttcgtc agcaaatcca atacgatttc gcgcgggttt
    12661 gcatcgggga tgttgccgga atagcccgat ttcccgatga cctcgaaatt gtgctgatat
    12721 atctgcgccg atttcgtcag ttcgtagttc gggctgcaca aataagccgt gcccgaataa
    12781 ttcaaggctt ggccttggtg cttcgcctgt tgcaggtgcg tccacaacgg ctgctcgtcg
    12841 ccgccgcgca taagcgtcag gcgcaactgt gccggcgaat cgaatttctc cttgtcgcgc
    12901 caaacccgcc ccacgccctg aatctcgccc tcgcacaaag caagcatgac ggcggcttcg
    12961 taggtatagg aaataccgac ctgtttcaca ccgccgccgc ccttgccgcc ctgacgcgtc
    13021 acggccttat gctcgatggt gacgaaatcg ccgtaccaaa tcaaattgcc cgccacgcgc
    13081 gtcctgccgt agatgacggg cagggtcagc ccctgtgatg accgctgaac ctgtaacgat
    13141 aaaatccgct cttcggcaga tgtaacggtt gacgatttac cacccataaa accacctcga
    13201 aacttgcaaa aattatcaaa atcgatataa tttacttata tcaaaacaga tataaaatat
    13261 gaaaccgtta aatttttcag gcggttcatt ggattgcctg cggcaattcc ccgaaaacgc
    13321 caaacaggcg gcaggttatc aacttcaaga aaagccggaa aaccgcgggc gccgattcgg
    13381 aatcggcgga aaaacgttac aacaaattga ttcgggaaaa gaaataatgg aaagccaaac
    13441 tttcgcctcc gtatttgacg cactgtgcga cacgcctgcc gaagccgcca atatgcggtt
    13501 gcgcgccggc ctgatgatgc acatcgccga taccgtccgc gaaaacggtt ggacgcaaaa
    13561 acaggccgca gaacattgcg gcctgacccg gccgcgcatc aacgacctgc tgaacgggaa
    13621 aatcgacaaa ttttcattgg atgcgctcgt aaacatcaat gccggactcg ggcagtgcat
    13681 ttccttatcc ttcgcccccg cgtaaagccc gtcctttcag acggccgcaa aatcaaaatg
    13741 aaaatgtaaa aaaccgaacc ccgcgcccga taagttcggc ttggccgatg tcgtccgaca
    13801 ccacgccgcg cccgatgtag ctgtgaataa ccttgccgcc gcccgccaat atgccgccgt
    13861 gcgaaaacga ccgcccgaaa cgccatactg caatgtcgcc cgcctgcggc gattccgttt
    13921 cacggcaaaa ttgcgtgacg aaccccaaat aacgctcgca atcgcggtgc agatgccaat
    13981 cttgagggta agggcgcggg tcgaaccctt cgggaagcag tccgaccgcc ccgtagacgg
    14041 cgaccaggag catcgcgcaa tcaacacccg cgcccttgac cattgcatga tgatggtacg
    14101 gcgtgccaag ccacgaccgc gcctcttcga cgatccgcgc cctcaaatcc gtttcggacg
    14161 gcatatcaca ccaccgtatc tgccgaaggg atgaacggga agccgcgaaa atgcacgata
    14221 ttgtcaaact tatccttgca ggtatcgcgc cgcttgtcgc agcccggata aaccttgaac
    14281 gcatcgcccg cctgcggcgg gtagggcagg cgaagggcaa gttcgaacgt gttgccgccg
    14341 tgtgccttga ccgtcctgct caaacccgcg ttgcgcccgc ccgcaaactt aatcacgccc
    14401 tgcgaaaacc agccgtcagg ctgcgtcaga ttgtgcttca gcacggttcc cgtccggctg
    14461 ttttcggtta cgcgcccgtt taccgtgaat ttctcacggt tgaccttgca gccgtcgtca
    14521 tagagcgtcc tcatacagcc cgcctgataa atgttgcgcg ggctggaaac gttcaaaagc
    14581 tcgatgtcgg atttcacgtc aaccttcacg gacgacctgc cgcccgatac gtccgacacg
    14641 cgccctgaaa aaatattcac cgcaccgacg gggcgcagtc cggcatcaaa aaacacacgg
    14701 tctatcctga cccgcgcgcc gtccaatacg ccgcccaggg cggcctccgc ccattgcagg
    14761 ccctcaagcc tgtaatcggg cgcggctgaa atctgcaggg tgttggaatc cacttccaat
    14821 ccgacggcgg tacgggttgc gccgcgcttg ataaccagct cgtgcgcccc gtaggcctga
    14881 ccgtcccaaa cgacgggcat atccgcgccg gtatgccgca gcacccggcc gcccgaaagc
    14941 gtaatggtgt acaaatccgc catctgaaac tcgtcgccgc cgtgcagcaa atcaatcagt
    15001 tcttttgtcg ccgccttcat agtttcacac tcgtaaactc aatctttttg gccgcccaca
    15061 agctgcccaa aacgttttca aaatccaccg tgtcagacgt aaacctcacg cggaaataaa
    15121 agccgcccgt ccatgtaatc gggcggcccg gcggttgcgg cgtgttgaaa accaaaacgc
    15181 ccttgtcggt aacggtgtaa tcgcgcccgt acgccaacgc cgtgccgccg accttgacgg
    15241 cgggccgttc cttgaccgcc gacacaggct cgataaatcc gcccatcgaa cggacaagct
    15301 gataacgcgc aacgccctgc accgtgtttc cgacaggctg gtcggttacg gcgttgtcgg
    15361 ccgggtcttc gtaaaggaaa ctttcaaagc tgcctttgcg ggcgttgaag aatcccgcca
    15421 gtttttccaa ctcgtttacg gacgcttttg tccgcaatac ctcgaacgac agcgaaaacc
    15481 gccattgcgg gtaggtgtag taggcggttc ggaactcacg gccgcttgcc gatttctgcg
    15541 tccccgtact ccataccgcc gttttcttcc gcccccactt caagccgggg aactcgggga
    15601 aaaccgcatt gcccatcaga tgattccttt cgctttgagc agtgcgtcaa attcgtcttc
    15661 agacaattca ttaccgccaa gcataccgac ggcttcggct tcgtccgctt cgctttgcac
    15721 actgcccgac gacggcttga cgcccatata cgacgctgcc aagatatgca cgggcggatg
    15781 ttcgcgccaa tactcgttca ggtgtttgat acgcggcaaa tccaagttgt cggcgacgta
    15841 gtcccacgtc cagccggtag aggcgcaaac gtgggcaatc accgcgccga aacttaatcc
    15901 gccgcccggg cctcccccgc ttgtgcggct tcctgctcct tgcgtttcaa gcccgaaacg
    15961 tccatcacgg cggcaaacac ttcgttcatg ttgccgatat cgatcaaatc ggccgcttct
    16021 tcgcgcgtta aatccgggta attgcgcttc agggcggcgt gggcgcaatc gataacggta
    16081 gagatttgtc cggcatccgc cgcattgccg tcaaatgagc cgatgcgctc ctgcaactgc
    16141 tccaacgcgc ccagcgcaat aggcggaatc acataatccg cgccgttcag ttctacggtt
    16201 acgcctttaa tccgtacggt catttttatt tccttgactt tggttgaata aaaaaagccg
    16261 ccttttcaga cggcatataa acattactcc tgaatccaca gcgtaccgat tttaaagccc
    16321 gcctcgtcgg ttgaggcggt aaagtcgatt tcggggacgg agaagtcgtc gtttttagtc
    16381 gagaacaagc ccaatttgcc gctggttacg ctttccagtt ccaacagggc ttttttgcct
    16441 ttgaactgcg tcaggtacct catcttaaac gtcggcgtgt tgcccgtcgc catattggtc
    16501 agctcgattt tcttcgccgg gggcatcgtt tgcgtatagg taaagctcgg ataaacggtt
    16561 ttgcctttat ccgagctgtt aaaggcgtaa acgcccgatt ccgaaaccgt atattggcct
    16621 tgtttcgggg aggatgctac tttgatataa gccgtgccgt cctggcccat cacgcccgcg
    16681 tcctcagcga acttgccgcc gccgggcgcg gctgcctgaa ggtaagaata attgtcgtca
    16741 tcgaggactt tgcccgtagt ttccgcccaa agcgccttca tcgtgccggt tgcatattcc
    16801 gtaccgaaaa acagggtatt gagggtcagg ccgttaatca acgcgccttt cattttgccc
    16861 gacaccttga ccttgccttg cgccacagcc aaaggataac ggttttgacc gtagaactct
    16921 ttcaattccg ccgacaaatc gacggacatt tcctgcaagc ccataatccg cacgggcgtt
    16981 gcgttctgta cacggttgcc gtaagcatcc gtaatcattt gggcgaacac ttcgccgctg
    17041 ccaaacgtca actgcatgac atccctttca aaaaaagccg cattacgcgg cgcaaatcac
    17101 aatcggaata atacaaaccg cctgttcgcc aagcgtcccc tcgtcggttt ccaccgtacc
    17161 ctcgacgcgg caatactcga tatccgcgcc gtccgccgtt aaatccgtct tgcccgtaac
    17221 ggggtggacg gcgttcacgg cattgcacac cgcgtcaatc agcggattca tgacgggcgc
    17281 gggcggttgg cccgccgtct ggacgtacag gtaaacatcg gcgcgcaaaa tccacttggt
    17341 ctcccgcccc gtcaatgtca acgcctgcat atcgccctgc gccataaata acgcaggctg
    17401 atcgtagcgt ttcacatcgt tccagtgcac cagcttccgg ctcttggtta cgaagccgtc
    17461 caatgcgtcc agcttcgccc acagcgcgga ataaaccgcc tcacgattca tttcaacacc
    17521 tttccaatcg atttttgcag gtcgtccgcg aacttcggcg tcaaatcgcg caatgccgaa
    17581 cggaggaacg accgctccgg caacttcaca tcacgggtat gggcgcggac gcggacatat
    17641 cgcggcggtt tcagcggctt gccgaacgcc tgcctgacct ggcgcagcga agccttaacg
    17701 ttgacactgc ccgcgaaacc atactcatgc gcgatgccgt aacggacgtt cgtatcgact
    17761 tcgcccgaaa cgacattgcc ggaaacattc acgcgctggt gtacggagcg tcgcaggtcc
    17821 cccgtcctta cccccaacac ccggcccgat aaccggttca gcacaacttc gctttgcagg
    17881 cgcaatgccg acctgcccac cgatttgacg accgcatctt gaacatcggc ggcatatgct
    17941 ttaaaaaccg ccgccagaat accgccgccg ataaattcca tcttcagcat cacacgccct
    18001 ttcgtttgta ctcattgagt atcgcaaacg ccgacggcgg cataccgccg gaattaccga
    18061 acgtagaaaa agcgacggtt tcgcctgcaa gcgtcttgct ccgtacgccc ttgccctcga
    18121 tttcgttcac gcgctgcacc gcgataatca tcaccgcttc gcgtatgtcg gcgggcatgc
    18181 gctcataacc ggcgcggtac gatacctcga catttttcag cccgcgcgcg aaaccttcgg
    18241 ggcgcatcag cagccagttg tcaaactccc agccgccggc atccgcgccg ttgattttga
    18301 cggacgacac cgacaggacg ggataattgt ccaacacgat gcggtctttg ccgttgccgt
    18361 cgtaccgccc gacataatcg gcggccagga ggctgcgccc gatataggct tccgccgccg
    18421 ccgacacgcc gtcaatgacg ctgaggaaga aatcgtcccg ccggtcatgt tcaacgccga
    18481 tacgctgctt gaactcctca agcgataccg gggcggccat cgttattcag ccttttcggc
    18541 ttcggcaggt tcggcttctg cttcggcagg ttcggcagct tcggcttcgg cttcggcagg
    18601 ttcggcagct tcggcaggtt cggattgtcc tgccggctgc ccggttttag gtttacgccc
    18661 gcgcttggct ttttcaggcc cttcggcagg ctcggaggca acgttgccaa aaccgaactg
    18721 atacaggaat tccgccgcct ccgacggcac ttccacgata ccgttttcat ctgccgcgta
    18781 gctttggctg ccgaaggaaa cgtcggtaaa cccttcgggc gcttttaatt tgactgtcat
    18841 ctcaaaatct ccaaaagaaa aagccgtctg attttcagac ggcatatcgg cttaacccac
    18901 gttggtaatc ataccgaagg caggcatgaa cataccttgc agcacctcgt ccgcatagac
    18961 gccgtattcg tacatacggg tgcgcagcgg ccattcgatt tggtaatact cttggcgcgt
    19021 acgcacttgc agcagattgc cgacgccctg aacgtaggcg ggcaggcggc ttgagtagaa
    19081 caggtaagta ccggcaggca ggttcgggtg taccacgatg ttcaggtcgt cgcccgtgat
    19141 cttgttcata tacgaaccga caacgacacc ggcgcggatg ttcgccgtat tgttcacgtc
    19201 aacgttcaac ttaatcaacg gcgcgccgct gtttccgata atcagcttgg tcagcgccgc
    19261 caaatcgcgg gcgttgacgt agacggtatc gggggacagg cggtatcggg taaagaaatg
    19321 cgcgaacgcc tcttcaaatt catacacgcc gcccgcgttg tcggaagtca ggccgccgcc
    19381 tttgttgtcc gaccagaacg cgcccgaatc aggcagggcg atttgggtca acaggccgtc
    19441 aaattccaaa atagaagtgg aattgtcttc ggacggcagg gaagcggcgg tctgggtacc
    19501 ttcggcatca gccgaaattt ccactttggc ggcagtggta accgcgccca gtttttcaga
    19561 accggccgca ccccagtacc aagcataggc aaccgcgccg cgaacagccg gaatcacggc
    19621 ggttactttt ttgcctgcct caataccgga aacggaagcc gccgcagatt ttcgggcgga
    19681 accgccgccg aacgtatcgg ttgtaccatc cgcgtttcgg cgtgtgattt tggcagggac
    19741 ttgggcagtt ttaatgttcg ggctttgtcc ggttgcaccg ttgttcgcgc ctgccacgtc
    19801 ccaatacgcc tgcaagccca aagccacaca gattacagac aaggtgctga cgccgagttt
    19861 gcccgccgcg tcggcggaaa cgacggcggt cggtgtaggt gtaacgcctg ccttcaggct
    19921 ggtgttgccg cccagcaaaa tcatttcttc ggcaaccata gtagcctgaa gggtttgggc
    19981 gaccgccaac gctttcacgt cctcgaaacc gcgcgcggcg taatccgctt caaaggttac
    20041 ttggttttcc aagccgatgg cgcggaattg cgcgttgcgt tccaccgttt cgtgattgat
    20101 gacgccgccg cgcctgcctt cgccgatacc ggcgcgttgg ttgccgacgt tgatattcgt
    20161 gacggctttc cagttcgagc cgatggtgcg gccgccgccc acgcgcggga tacggttgcg
    20221 caacggggtc aataccgggt agagtttttg agacggcgcg gaaaggtcat aggtttgcag
    20281 gccggcggta aagccggccg gctgcgtaaa acctttgtcc ggcggcccgc cgcccgcttg
    20341 tgccgacttc atcagttcaa tcgtttcttg tgtgagttgg ttcacgttca tttatcgctc
    20401 ctgataataa aaaaaccgcc tgtaagcggt gttacagacg gcctgtttgt tttgccttaa
    20461 tgagtgtcgc cacgtcatcc agcgtgccgt cattctttac aatcggctga aaacctttta
    20521 acgggtcttc gccgttatct tctgccttac tgatagcttt cgtactgcct ttcggcggta
    20581 ctgcctgttt cttcaggctt tcgatttccg cctgtgcttt ggcaagggcg tcattcgatt
    20641 ttttcagcgc gtcttgtgcc tttgccagtt cgtcagccga ttccgctttg gcaagatcgc
    20701 ctgatttgcc ggctttggcc gccaaaccgt cggccggctt atcggcttcg cttgccgcca
    20761 acgctttcag cgattcggca aggccggccg ccgattcttt gatttgcgcg gtaacggctt
    20821 catcgatgtt gtcgtaggat gcgtcctcaa ccagccattt cagcgacatc aatacgtcag
    20881 ccggtgattt gacttggtac attgatttgg cggccggccc gcctttcggc ttgtcggctt
    20941 tagccaatac cgctttcaag gcggcgattt cagattcgga caattcgacg cttgccgatt
    21001 ttccggcttc gtcctttttg ccggcgtcct tatcgccttt atcttcggtt ttaggccctt
    21061 tatcgccgtc tttaggattt tcatcttcct cttcggttgg tttgtcagac ggcttgccgt
    21121 ctttatccgc cgcccctgcc tcatctttcg gtttgtccgc cttaaagcag gtaaacaccg
    21181 cgtcgggatt ggcagggcgg tcgacaaggc tgatttccgt cagcttcaag cccgtgattt
    21241 gcgacttgtt caaatcatcg cgggcggtaa ccctgccgcc gatggaaaag cctttgtaaa
    21301 cgcctgtctt gactttcgtc acggcaacag ggtcaacgat atgcgccccg aaaaatgtgc
    21361 gcccgtcgtc ttccacgttg atttcaatcg ccgttcccgc cgcgtttgag ccgtgcatct
    21421 cgcgcaccgc gccgaacttc atataatcgg gaatcgccgc cttcattgct tccgccgcga
    21481 cgacttcgcc gtccgaatcg accgcttcgc tcgaggcata cccccaaact ttgaccgtgc
    21541 cgtcgtcctg cgcctccgtc ttggcgattt gcgcgtataa cttcgtcatt ggtttgctcc
    21601 aaaaaaaagc cgccccgcta aggggcggca aaacacaccc gcctgacccg aaggaatcaa
    21661 gattcaggca tatcccctgc caaaacaggc acaaccgcgc accggcagtt cggatgaccg
    21721 ggtatcgtca acgcaccgtg ggagaaaggc tcgtgcaaac cgattacgcc catcccgccg
    21781 ttggcattgc agacatcgga caccttgtcg tcttttgcgg ttatccactg cttgccggca
    21841 accagccccg ttccttccca gcctatcagg ttgcccatac cgtccgccat cgccgtttcc
    21901 gttcgggcaa tggttcgggc gcgtgcgttg ccgaaagcat gggattcttt caggcggcct
    21961 gccaattcct gcacactgtc gccgtttcgc atggcttcgg ctacttgggc gcgtatcatt
    22021 tcgcgcgtcc cttcggtgat ttgccactcg gcggcaggat ttcggacaag cccgccgccc
    22081 gcccgcttca tgccgaccat ttcggcggcg cgttcatgcg cccacttgac ggcgcggctg
    22141 cgaatgttcg taaccatacc ggcggcaggt tcaggcatta cgcgcaacaa ggcggcaacc
    22201 gccccgtctt ccgcaacacg cctgattatc ggctcgacca catcggacaa tcccgaccaa
    22261 tcgccaaaat ccaagccgtt tgcaacaacc cctgccgcgc ggctcaattc cgccgccaaa
    22321 tccccggcct gccaatcgac ggccgcaccc tcaatcagcg ccgcgatttg ttcggccaag
    22381 ccgtcaatgc gtgtcagcaa ataagcctca ataagcgcgg cagattcgtc ttcgctcatc
    22441 gggctttccg actttcccaa cttttcagcc ccttggttcg gcggctcttc gggctttcgg
    22501 ccgtcttgct tatccggttc aggctgcccc tgccccggta acggctcctt gcccagttcg
    22561 gcgcggattt cgtcggcggt caagatgcct gcgtttttgt agatggcgta gatttcagcc
    22621 tgttctttcg ggttgagcga ttcctccccc ttccagacaa actcatacgc cgccatatcc
    22681 atgtaacggg caagcacgtc gtcaatcagg gcttttaccc agtttttcag gctgcccatg
    22741 ccgtctgaaa gcgactgctc gcggctcgtc tctgccacgc tgcggtttac ctgcgccacg
    22801 aacggcgtag gctcgacact aaacgcaaag cagacgacac gcgccagcca ttcgtcgtaa
    22861 acgtccttca acggcggctg cttcgtctcg cggaagtttc gggacaactc gcccggcacg
    22921 aaacgcattt tgcgccgctg cgccgtttcg cccgacagca gcaaatccca gtattcttga
    22981 aaccgcctga tgtcgtccgc cgaccacgtt tcaggcacgc cgactaaagc atcgggcacg
    23041 ctgcccgccg tgtagtattc cagcgcgtga acctgccgtt ttagggcgat attcacggtc
    23101 atgatgattt gctcgacggg cgaatagccg taaaccttgt aacttcggtt attccgcgaa
    23161 cggtaaatca actcgtcagc cgtgtaatcg accgccgcca tgccgtgcag gatttgctga
    23221 tacgccgtat cgggcggtaa cggcatacgc cccgtattgt ccaaaacgcg cttaatcgtc
    23281 gccccgtcca tcacttcgag ggcgtacaag ccgccgccca gtgttttgcg cgggtagatg
    23341 cacggcgcgt caataacaaa caggtcttcc agcaagatgc gcagccagtc cgcccacgta
    23401 tgttctttat cgggcgaccg gaagaacgca acggcttcat cgacctttcg gtctttccgt
    23461 tgcgattcgt cgtcttcggt tgattcgacg tcgcgctttt ggattgtcca cttaaggcac
    23521 tccatttggt ctttacgcgc ctcgatcacc aaacgcaata catcgtagtt gtcggcaagg
    23581 gcgcgtaatt gcgcaaagcc taccgcttca cgttcgcgcg gcttggaatg cccgacgttg
    23641 tagaacggct cgtaatcaaa ccgccgcccc tctgcctgct gcgcggcagg ggccggaggc
    23701 tcgcccgcgt cgaaccaccc gtccgcgttg ccggtaaagg cgtaacggac gccggcggca
    23761 acgcgggcaa taaagccttg cgataaaggt gtctttttac tcatttcaca gcctcgacct
    23821 gcgatcgcag gaaatcaatc atgcccgtcc gggtatccag cagctcgcca aacgcacggc
    23881 ccaaacagtc tatttggtcg tcatgccggc cgttcgggaa catgcgcatt tccgcaatca
    23941 gcgcgtccgt gtcccatgtg ccgtcatcca acaccatcac attgccgatg ttgacctgtg
    24001 ccgcgaacgg tccggcgcgt gtaaccttgt cgcccgattc ggggccggcg gatacggaaa
    24061 aacccgccaa ctggcgggtc aggtatagtg tttgcgattt gcctgcctgc ccggggtctt
    24121 gcgggatgga tattttcgtt ttcacgccgt ctttttgcgc cgtgttgcgt aatatcctgt
    24181 cccgctcgtc cgcgccgtac cggccgcgca cgacgttggc gatgatatac cgcccgtctt
    24241 ccgtaacgcc aagcctgccg cctgccgtgt agtcgccgcc gtttgcggtt gaagccaagt
    24301 cccacgcgcg tacccatctg atattcccgg caggcaacgc cttgacaaat tgcaggttgt
    24361 caggcttgaa cgtaccgccg tcaggcggcg caggtttttg caaatactgc ccggcaaaca
    24421 catacggcgc ggcctgctcc attcggcgca gtgtttcaat atcatgcttt tcaggccaca
    24481 acgccgtgcc gtcttcctga atggcaggca ggcacaaatg ctcccactct tcgccgttgc
    24541 cgccgtcaag cagccagccc gccaagtctt tctcgtgcag gcgttgcata atcaggataa
    24601 tcggcgtgtc agggctgttc ttccgggatt cgaccgtgtt ttgaaaccag tcgatgacgt
    24661 tctgccgcct gacctcgctt cgcgcttcat ctgctttgtg cgggtcgtca atgatgatgc
    24721 agccgccgaa tccctcccga tgcctgcccg caccgaaacc tgtaatcgta ccgcccgcac
    24781 ctgttgcgta catcacgccg cctgcggttg ttttccagtg atggccgctt tcgcctgcca
    24841 gtgccaaatc aggaaaaatc gcccgatact cttcgtgttg caccaagttc cgaatctgta
    24901 cggagttatt gaccgccagc gccgccgaat agctcgcgtg gataaactcg caatcaggca
    24961 cgcgccccat cgcccacgcg ataaagttca cgaccgcgat ttccgttttc gagtatcgcg
    25021 gcggaatatt gataatcagg cgtttcgttt cgccgttgaa aacacgctcg agcgcgttgc
    25081 agattggcgc atggtgattc gcccgctgcc aaacatagcc gcgcctttga taaaacatcc
    25141 ggcgcgtgaa catatacagg cttgcagaac ttaaactgcg aattactgat gtttcaacat
    25201 cgtcgaattg ccctagtgcc atttcattct aacttccttt ggagaaacgg ccatacagcc
    25261 cctcttatga ttagcgtttt atgctaaacc ctacgcaaaa catcttccgc tatcttgcga
    25321 tattcctcgg catcaagccg tacagccgcc ctcatgctcc catcgcccga tttaacgtca
    25381 agctccgatt tgtcgccgta tttcttcggc gcaatcttgg aagccgccca ctttcgggca
    25441 tctatctgca atttcgcctt tgaaaccgcc gcgctctctg cttgtgcact atcggcaatc
    25501 tcgatgattt cttcagcgaa atagtccgcc tgcttttctc tcgcgcgcgc gtattggtgg
    25561 cgaaattctt cattagcttc caaccaacgg taaatagttt tcattggcgg cacaccatct
    25621 tcggcgcata ttgaacgtag gcttctgcca tttgcgattt tttcgcatat cttatcggcc
    25681 atgtctttcg tgtaatctgt cggacggcct aatttgcgtt tcgtgccgct cataaaccct
    25741 cctcaaaaaa gaaaccgtct aactctgacc cctctcagaa ttagacggca aacacacact
    25801 cgccacatag gaaaaacgga acgccgctac ctgtacaggc agaagctcaa attcggacag
    25861 cctaaaacgc aaaaacccgc acattgttat gtacgggctt aaaaattcat atccttcggg
    25921 cgtgcgaaaa tccccgcaag ggtaacgatt tgaattatac acctattgcc ggaaaaaaca
    25981 acaggccgtc tgaagattca gacggcattt gtattcccta ctgcatcaaa ccgccgacag
    26041 gttgcggatt tcgggcagta tcgggcggat tttttctgcg tgttccgcgt cggcgtgtgc
    26101 gcttaaggct tcgagggcgt ttgcggcggc tttgaggcgg ctgcgcgttt ccgcccagac
    26161 cgtccacatc gtaaccgcct gcctgcagcc gagctgcttc agcggcaggg aaacgtctct
    26221 gcccatttgg atggcccgcg cgccgtagct gacggcgacg gcgaggtcgt acagggcgtt
    26281 gccgcttatg ggcagggcgg gttgcggcgc gggtaacggt tcgcggtcga ggacttcgcc
    26341 cgtcaagccc gtgtgcagtg tcaatgcgtg gacgtaggcg acggcttcgg gcagcttccc
    26401 ggcggggatg ccttcgacgg attcgacgtt gaagcgttgg tgtatcatac tgtacgctga
    26461 ggagtagtct atgcgtttgc gcccgacaag cgcggcgacg gcccggcgca atccggtacg
    26521 gtcgtcggcg gtggtttttt gtccgacttg gtagccgcct gttttgcgga tggtgggcag
    26581 gacttcggat gttacccatt tgcggaattt ccaagcggtt gagccttgtt ccattgcttt
    26641 acggctgcga agaatcaaaa tatataaacc gctttcgttg atgatgttca cgttaccgcc
    26701 gcctccgtga atttcagacc gccctatgtt aaacatagac cgctcgtcat catctaattt
    26761 ttcaagtgct tgcgttgggt tctggatttc taaagcctta catacatcgg cggcaacaaa
    26821 ccaagtcaag ccgtcttttt caaaggcacg aacgggagaa gtagtattga aattaaatga
    26881 ttgaattacg ttcataatga agtttcctag tgagttttct taatgcccgt tagggcggac
    26941 gcgtggttaa gaaccctcac tagatgggcg gacttattcc ccttacgggt attgtattcg
    27001 tcgcccacgc gtccataaga aacttcggtt gtgccatcga aacaaacaac acgaaaggaa
    27061 acttacagat atgaaaaaat cacattgacg gagtgattgc cgctagtgtg tggttcttac
    27121 gccacgaaca ggaatataaa acaaaccccc ctgcacatgc aaggggtttc gttaccttat
    27181 ttgggtttgc gtttaaaatt ctcgtcatca caacagaatc ggtaagaatc aataaccaac
    27241 caaacaacgc gaagcagatt ttcaggcgta tcaataataa cttgattgcc tgatacctca
    27301 agtccgcatc gttcgatatt cgaaatatct gactcttcca attcgatagg gaagattaca
    27361 gacggccttt gtttgttgtc aaaataccgt aatatccacc tgttactttt accatcaacc
    27421 aatacgccga aatagctttc cgtatctttt gcctcaatac tcgcatcatc aggaagaatt
    27481 gatttaacca aatcaaacaa ccttctttct gaataagtgg taacgatttt attgttttcg
    27541 gggtcgataa tcggcgcggt cgggtcttcc tgctccttct caacaggggc ggcctcctcc
    27601 tgcactttcg gcgcactcag tcccgataca accattgaac taaccgtatt ttgaacagcc
    27661 tgtttgacga tatgacggat gctttccaaa tatcgttggg taaactgccg ttgaatattc
    27721 gcccttcccg ccacataacg gacgaaatcc aaatccacct ctttcaagct ttctgtaatt
    27781 gattctgtaa atgccgtcag gtatatgctt tcttccgcaa ggcttcgcaa tgcatcaggt
    27841 tgaaacttgt cgtgcctgaa ctgatacagt tgcgccatat cattttcatt caacatcgta
    27901 acatcaacgg tcaaaaacgg ctccgaatcc ataatattct tgttggacaa atcggtaaaa
    27961 aatcgccatt cccggccgtt ggtaatcgcg caaatggcta tttcaggcgt ggcattgaag
    28021 taacgcgaca actgcggaca atgattggac aaattttcag tatatgattt tgcttcgata
    28081 aacataaccg gtgcgccgtt gcaaaacaat gcgtaatcga cccgctcccc tgattttgca
    28141 cctgggaaat ccgcctggta ttccgcccga accttattcg gatcgaatgc cgaaaaacct
    28201 agaatatcca ataaaggcaa aatcaaagcc tgttttgtcg tctcctcggt agtacagata
    28261 tgtgcaacct tcttgacatg ttccgcatgg gaagcaatcc gttctttaaa taccgcactt
    28321 acagccgccg cattcatgtt aatctccttg gttaaatttg attgggcaaa ttcccacccc
    28381 attaagaagt gttaattttc gcaatcatag cgcaaccgaa aagaaaatca aaccttctca
    28441 aacagcaaat caaaatcatt ccctgccgcc tgccgtatcg ccctatacca cgcggccaag
    28501 cccaaatccg tctgcaaatg caggggcccc tcgccgcgcc gcctgatttc agcctgcaaa
    28561 cgttcctcgt aggctgcctg cgacttcgca ccgatgccga acgaaatacg gacggcctct
    28621 tgttgcggcg catccacctt cgcccatgcc tgcaaagtaa gaaacatggc atcttcgccg
    28681 tatctcaagc cgatttcagg cttatgcggg caagcttcct cccccatata acggccttcg
    28741 atactcaaac atctatttag gctgcgcgta tcacggtaac gacgctcaaa agcacgcgcc
    28801 aagtcgttca taaattcaaa ttcttgctga ttcatagctt gattaaccct ttttcatgca
    28861 acaaaaccaa agtccgcatt acaccttccg cgaaggccgt ctgaatttcc ccttccgcac
    28921 aatccgccaa gcggttctat acatcaaaac tcccaaatga ttccaaactc taccgcagcc
    28981 cgcgcctgca ccgtttcgga tatggcggac ctcggcagga acgccgaacc gcccgcaatt
    29041 gcggcaaaca atacaaccta tatccgccac tttttgcaga tgtgattttt ccgcttcggt
    29101 tttacttttc
//
LOCUS       DAXFWM010000019        27773 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000019 DAXFWM010000000
VERSION     DAXFWM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27773
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.019"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            710..973
                     /locus_tag="ACFI8B_000822"
     CDS             710..973
                     /locus_tag="ACFI8B_000822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834089.1"
                     /translation="MSSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKI
                     KKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            1053..2501
                     /gene="gnd"
                     /locus_tag="ACFI8B_000823"
     CDS             1053..2501
                     /gene="gnd"
                     /locus_tag="ACFI8B_000823"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC3834090.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWGLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2564..3835
                     /gene="waaA"
                     /locus_tag="ACFI8B_000824"
     CDS             2564..3835
                     /gene="waaA"
                     /locus_tag="ACFI8B_000824"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC3834091.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWVGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3876..4325
                     /locus_tag="ACFI8B_000825"
     CDS             3876..4325
                     /locus_tag="ACFI8B_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC3834092.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4358..5332
                     /locus_tag="ACFI8B_000826"
     CDS             4358..5332
                     /locus_tag="ACFI8B_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC3834093.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5401..5476
                     /locus_tag="ACFI8B_000827"
     tRNA            5401..5476
                     /locus_tag="ACFI8B_000827"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5434..5436,aa:Lys,seq:ttt)
     gene            5696..6949
                     /gene="murA"
                     /locus_tag="ACFI8B_000828"
     CDS             5696..6949
                     /gene="murA"
                     /locus_tag="ACFI8B_000828"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC3834094.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            7030..8223
                     /locus_tag="ACFI8B_000829"
     CDS             7030..8223
                     /locus_tag="ACFI8B_000829"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC3834095.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGAAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMATKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(8275..8523)
                     /locus_tag="ACFI8B_000830"
     CDS             complement(8275..8523)
                     /locus_tag="ACFI8B_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC3834096.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8616..9533)
                     /gene="ftsX"
                     /locus_tag="ACFI8B_000831"
     CDS             complement(8616..9533)
                     /gene="ftsX"
                     /locus_tag="ACFI8B_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC3834097.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9530..10180)
                     /locus_tag="ACFI8B_000832"
     CDS             complement(9530..10180)
                     /locus_tag="ACFI8B_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC3834098.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10374..10856)
                     /locus_tag="ACFI8B_000833"
     CDS             complement(10374..10856)
                     /locus_tag="ACFI8B_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC3834099.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10980..11333
                     /gene="arsC"
                     /locus_tag="ACFI8B_000834"
     CDS             10980..11333
                     /gene="arsC"
                     /locus_tag="ACFI8B_000834"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC3834100.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11330..11986
                     /locus_tag="ACFI8B_000835"
     CDS             11330..11986
                     /locus_tag="ACFI8B_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC3834101.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12102..13496
                     /gene="gltX"
                     /locus_tag="ACFI8B_000836"
     CDS             12102..13496
                     /gene="gltX"
                     /locus_tag="ACFI8B_000836"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC3834102.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13517..13923
                     /locus_tag="ACFI8B_000837"
                     /pseudo
     CDS             13517..13923
                     /locus_tag="ACFI8B_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13927..14418
                     /locus_tag="ACFI8B_000838"
     CDS             13927..14418
                     /locus_tag="ACFI8B_000838"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3834103.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14694..17288)
                     /gene="mutS"
                     /locus_tag="ACFI8B_000839"
     CDS             complement(14694..17288)
                     /gene="mutS"
                     /locus_tag="ACFI8B_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC3834104.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17719..18723
                     /gene="gap"
                     /locus_tag="ACFI8B_000840"
     CDS             17719..18723
                     /gene="gap"
                     /locus_tag="ACFI8B_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC3834105.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18875..19555)
                     /gene="tsaA"
                     /locus_tag="ACFI8B_000841"
     CDS             complement(18875..19555)
                     /gene="tsaA"
                     /locus_tag="ACFI8B_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC3834106.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            20015..20650
                     /locus_tag="ACFI8B_000842"
     CDS             20015..20650
                     /locus_tag="ACFI8B_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC3834107.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVDNPVDMLKPTGLSDADLTWVAHAMVGTCGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNK"
     gene            20687..21655
                     /gene="waaC"
                     /locus_tag="ACFI8B_000843"
     CDS             20687..21655
                     /gene="waaC"
                     /locus_tag="ACFI8B_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC3834108.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21881..22630
                     /locus_tag="ACFI8B_000844"
     CDS             21881..22630
                     /locus_tag="ACFI8B_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC3834109.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22641..23576
                     /locus_tag="ACFI8B_000845"
     CDS             22641..23576
                     /locus_tag="ACFI8B_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC3834110.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23639..24250
                     /locus_tag="ACFI8B_000846"
     CDS             23639..24250
                     /locus_tag="ACFI8B_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC3834111.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCLLTVSLSLANYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(24302..24607)
                     /locus_tag="ACFI8B_000847"
     CDS             complement(24302..24607)
                     /locus_tag="ACFI8B_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC3834112.1"
                     /translation="MDEIQSKYREWCHLLLQLEENIRRWKYAVALIRDMDNFYTHEYQ
                     VRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSVDTLDRYTQEN"
     gene            24777..26048
                     /gene="purD"
                     /locus_tag="ACFI8B_000848"
     CDS             24777..26048
                     /gene="purD"
                     /locus_tag="ACFI8B_000848"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC3834113.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            26082..26684
                     /locus_tag="ACFI8B_000849"
     CDS             26082..26684
                     /locus_tag="ACFI8B_000849"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC3834114.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPFENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26699..27214
                     /locus_tag="ACFI8B_000850"
     CDS             26699..27214
                     /locus_tag="ACFI8B_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834115.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(27366..>27773)
                     /locus_tag="ACFI8B_000851"
     CDS             complement(27366..>27773)
                     /locus_tag="ACFI8B_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC3834116.1"
                     /translation="LSFVLLPILITACSTPDKSARWENIGTISNGNIHTYINKDSVRK
                     NGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSSLQLFDTKNTEI
                     STQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         6653 a         6370 c         7840 g         6910 t
ORIGIN      
        1 gccttttcaa agttttattc atattttatt ccttaagttt gccttcgcgg gacggcgcgc
       61 cgcgaacgcc ggggttcggt aaaccgcccg attccgcgcc cgccgaattg ctgattgaaa
      121 agcttacctc cccattttaa ctttgcgcac ttatcacggt aaagcgtttt ttagcaaacc
      181 gccgcagatg cccgaccggt ccggattccc gcctgcgcgg gaatgacggc atcggtttga
      241 cggtatttaa ttgaattgcg gaaattgacg gattcagtga gattggcggg atgaagccta
      301 ccctatagcc cgccttttat agcggattaa caaaaaccgg tacggcgttg ccccgccccg
      361 gctcaaaggg aacggttccc taaggcgccc aagcaccggg cggatcggtt ccgtaccatt
      421 tgtactgcct gcggcccgcc gccttgtcct gatttttgtt aatccgctat gcttatacac
      481 aaccagccaa aagccatctt attgaaatat cataagattt actcaaaaat cttattttct
      541 ccaccactta tgcacaaact cggatacaca ctctgccaat atgcagaaaa aaagacattc
      601 gcgccgacac cttttcatcc ccacaagttc caaacacatt tccggcttaa atatccacag
      661 cattcccgtt cttaaaggca ccggcagcca ttcggtaaag aaaagcggga tgtcgtccgc
      721 acaacaagca aactatccac aaaacctatt ccccacgaaa aacaatccac aggtatgcac
      781 aaaagggaat cctccttacc gacacccttt taaaacggca attcagatga ttttaaaaat
      841 aaaaaaaagt tacccacaga aaattcccca tatcaatata atcatcgttt tattatttaa
      901 accttatatt ttatttataa cccttcaggg aaacggcacg gcaaagcgta aaggcatctt
      961 ttcaaaaaat taaaaagtta aggattgtta aacaagtgta aatcaggtag agttgcagcc
     1021 gataaattaa atctataacg gagcttcctg caatgaaagg cgatattggc gtaatcggtt
     1081 tggcggttat gggtcaaaac ctgattttga atatgaacga ttgcggattt aaggttgtcg
     1141 cctacaaccg gacaatcggt aaagtggacg aatttttaaa cggtgcggca aaagagacgg
     1201 gcattatcgg cgcgtattcc ctgcaagact tggtcgataa gctggcaaaa ccgagaaaaa
     1261 tcatgatgat ggttcgcgca ggttcggtgg ttgacgactt tgtcgaacaa ctgcttccgc
     1321 tgttggaaga aggcgacatc attatcgacg gcggcaatgc caattacccc gacacgacac
     1381 ggcggacaca ttaccttgcc ggaaaaggga ttttgtttgt cggcgcgggc gtatccggcg
     1441 gcgaagaagg cgcgcggcgc gggccgtcca tcatgccggg cggggataag cgcgcttggg
     1501 aagcggtcaa gccgattttc caggcgattg ccgccaaaac gccgcagggc gaaccgtgtt
     1561 gcgactgggt cggcaaggat ggcgcgggac attttgtcaa aatggtgcac aacggcatcg
     1621 aatacggcga tatgcagctg atttgcgaag cgtaccagtt tatgaaagac ggtttggggc
     1681 tgtcctacga cgaaatgcac cgcgtgtttg ccgagtggaa caaaaccgag ctggattctt
     1741 atctgattga aatcacagcg gcaattttgg gctataagga cgaaggcggc gaaccgctgg
     1801 ccgagaaaat cctcgatacg gcagggcaaa aaggcacggg caaatggacg ggcatcaatg
     1861 ccttggattt gggcattccg ctgacgctga tttccgaagc cgtattcgcc cgctgtgttt
     1921 cgtcgttcaa agaacagcgc gtgcagaccg gcaagctgtt cgcccgaacc gccacgcccg
     1981 tcgaaggcgg caaacaagaa tgggtcgagg cgttgagaca ggctttgctt gcctccaaaa
     2041 tcatttccta cgcacaaggc tttatgttga tccgcgaagc gggcgaaagt tacggctggg
     2101 gtttggacta cggcaacact gcgctgctgt ggcgcgaagg ctgcatcatc cgcagcgcgt
     2161 ttttgggcaa tatccgcgac gcgtatgagg ccaatcccga tttggtgttc ttgggtgcgg
     2221 atccgtattt caaaaatatt ttggaaaact gcctgccggc atggcgcaag gtggttgcca
     2281 aggcggtcga atgcggcatc cctatgccct gcatggcttc ggcgattacg ttcttggacg
     2341 gctatacgac cgaacgcctg cctgccaacc tcttgcaggc acagcgcgac tacttcggcg
     2401 cgcacactta cgagcgcacc gacaaaccgc gcggcgaatt tttccatacc aactggacgg
     2461 gcaagggcgg agataccgct tcgacaacct acgatatttg attccgttgc cgaaatgccg
     2521 tccgaaccgt tcggacggca tttgttttgc aggaaataga aagatgttcc aatggcttta
     2581 tgatgtattg tggctgcttg cgccgatatg gatacggcgt tatttggaca aacgctccgg
     2641 aagtgccccg gcataccggg cgcataggga cgagcgtttc ggcaagccgc atccgaatcc
     2701 cgttaccggc gcggtttgga tacacgccgt ttcggtcgga gaaacgcgtg ccgcccagcc
     2761 cttgatacgc gagttgcggc ggcgttttcc cgatgcgccg ctgctgatga cgcagatgac
     2821 cccgacggga cgggaaaccg cgcaagttct gtttcccgat gcgcaatgcc gctatcttcc
     2881 gtatgacaaa aaaacgtggg tacggcagtt tttgcgcgaa caccgcccga tgttcggcat
     2941 tttgatggaa accgaaatct ggcccaatct gatgaaggaa tgccggcgag cgggtgtgcc
     3001 gctgtttttg gcgaatgcgc ggctgtcgga aaaatcgctg aacggttatc tgaaagtccg
     3061 ccgcctgatc cgtcccgcag ccgcttcgct gacgggctgt ctggcgcaga cggaggcgga
     3121 tgcggcgcgg ttggcgaaat tgggcgcggc atccgtgcag gtgtgcggca ataccaaata
     3181 cgacctgatg ccgtctgaag acatgaaaac gctggcgggg cagtttgaaa aacgcatcgg
     3241 aggccggccg gttgccgtgt gcggcagcac gcgcgtttat cggggcgaag acgaggcgga
     3301 aaaactgctg gcggcgtggc aacaatatcg cggcgatgcc ctgctggccg tcgtgccgcg
     3361 ccatcccgag cattttcaga cgacatttga aacggcgaaa cgcttcgggt ttaaggttca
     3421 gcggcgcagc gacggtttgc cggtcgaacc cgatacgcag gtgtgggtag gcgacagtat
     3481 gggcgagctg tatgcgtatt acctgtgcgc cgatgtcgct tttgtcggcg gcagtctggt
     3541 cggttcgggc tgtcagaaca tcatcgaacc gctttcctgc ggcgttacga cgatattcgg
     3601 cttttcgacc tacaattttt ccgaagcctg ccggcacgcc ttggcatcgg gtgcggcggt
     3661 tcaagtcgaa tcggcggacg cgtggcgtga agccgttgaa aaaaacttat ctggcgaggg
     3721 gggcggaatg cagatgcagg cgcgcgtgga cggctttatc gcacaacatc gcggagcggg
     3781 cgcgagaatc gccgaggcgg tgcgggaagc ggtatgcgga catcgggggc gatagtgata
     3841 caatccgtat ccgagttttc cggttggagc atcgtatgag tatttatgcc gtcgcgcaca
     3901 tcatccacct gtattgcgcc accgcctttg tcggcggcgt gttttttgaa gtgctggttt
     3961 tgtccgtcct gcatacggga cgggtgtcgc gcgaggcgcg gcgcgaagtg gaaaaggcaa
     4021 tgtcttaccg cgccgtcagg gtgatgccgt ttgcggtcgg actgctgttc gccagcggca
     4081 tcgtgatggc ggcaaaccgc taccttccta tatcgggcga accgtttgcc acttccttcg
     4141 gtacgatgct gacgctgaaa atcctgttgg cgttcagcgt gttggcgcac ttcgccatcg
     4201 ccgtcgtcaa aatggcgcgt tccacgctga cggtcggctg gtcgaaatac atacacgccg
     4261 tcgtctttac ccatatgctg ctgattgtct ttttggcaaa agcgatgttt tatatcagct
     4321 ggtaacacaa ctacatttca aatcaggaga ttgaaccatg acagaatatc ccggcatcgg
     4381 gtcgccgttg ttttacggcg ttttttttgc ggcagtgctg gtcatgattg ccttggatat
     4441 gttttcgctg aagaaaaacg gcagccataa agtcggcatc aaagaaacct tggcatggag
     4501 cggcttgtgg gttgccgtat cctgcctgtt cgcaggctgg ctgtatttcg aactcgccgg
     4561 caatcccggc tacggcgcgg cagccgcaaa agaaaaagta ttggaattct ttaccggcta
     4621 cattttggaa aaatcgttgg cagtcgacaa tattttcgtg ttcctgatga tattcggcta
     4681 cttcaaagtc gcaccgcagt ttcaacaccg cgtgctgctg tacggcgtat tgggcgcatt
     4741 ggtattgcgc accgtcatga ttttcgtcgg cgcggcactg gttcggcagt tcgagtggat
     4801 tctgtatctg ttcggcgcgt tcctgctcta taccggcata cacatgatga agcccgaagg
     4861 agatgaggaa gaggatttgg caaacagcag gctgctgaat gccgtcaaga aagtcgttcc
     4921 ggtcggcacg gcgtttcacg gcgagaaatt ttttaccgtc gagaatggca aaaaaatcgc
     4981 cacgccgctg tttttggtgc tgattatgat tgaattgagc gatgtcgttt ttgccgtgga
     5041 cagtatcccc gccgtctttg ccgttaccac cgatccgttt atcgtgctga cttccaatat
     5101 tttcgctatt ttgggtttgc gggcgatgta tttcctgctg gcggatgtgg cggaacgctt
     5161 tatcttcctg aaatacggct tggcattcgt gttgggtttt atcggtgtga aaatgctggt
     5221 gatgcattgg gtacacatcc cgatttccgt ttcgctgtcg gtcgtgttcg gcgcgttggg
     5281 tgcatcgata ctgacctcgt taatttatac gaaaaaacag cctgataaat aaaacgccgt
     5341 ctgaacgccg acaaaatggt tgacagcatc aggcttgatg tataaacttt caaatttttc
     5401 gggtcgttag ctcagtcggt agagcagcgg acttttaatc cgttggtcga gcgttcgaat
     5461 cgctcacgac ccaccaggta aagcaaaaaa gctgagtttt caaaactcgg ctttttttgt
     5521 tttccgataa tccttgttcg gaaagcgcgg catttcgtca aattctttgg tgcggcttcc
     5581 gtatttttcc cgaaaatccc ttataattcc gcctctgcaa ccttcatcgg gtgatgcttt
     5641 atgcattgtc ccgagtattg aaatattagc aagtacccac caggagggaa caaacgtgga
     5701 caaactgaaa atctccgcaa acggcccgct caacggggaa ataacggtct cgggcgcgaa
     5761 aaacgcggca ttgccgctga tgtgcgcggg tttgctgaca tcgggtacgt tgcgcctgaa
     5821 aaacgtccct atgctggcag atgtcgcaac cacgcaaaag ctgcttcagg gcatgggcgc
     5881 gcgcgttctg accgacaata tcagcgaatt tgaaatcaac ggcggtacgg tcaacaatac
     5941 ctgcgcccct tacgaattgg ttcggacgat gcgcgcttcg attttggtgc ttggcccgac
     6001 gctggcgcgt ttcggcgagg cgcaagtcag cctgccgggc ggctgcgcca tcggttcgcg
     6061 ccccgtcaat cagcatttga aaggcttgga agcgatgggg gcggaaatcg ccatcgaaca
     6121 cggttacgtc aaagccaaag gcaaactcaa aggtgcgcgc gtggcgatgg atgtcgttac
     6181 cgtcggcggt acggaaaacc tgctgatggc ggcgacgctg gcggaaggta cgacggtttt
     6241 ggaaaactgc gccatcgagc ctgaagtggt cgatttggcg gaatgcctgg tcaaaatggg
     6301 cgcgaaaatc agcggcatcg gaacatcgac catgattgtg gaaggtgcgg gcgagctgta
     6361 cggctgcgaa cacagcgtcg tccccgaccg gattgaagcg gggacgttcc tgtgtgcggt
     6421 ggcgataacc ggcggcaggg tggttttgcg gaatgccgcc ccgaaaacga tggaagtggt
     6481 gttggacaaa ctggtcgagg caggtgcggt gattgaagcg ggcgacgatt ggatcgccat
     6541 cgatatgcgg cagcgtccga aggcggtgga catccgcacg gtcgtccatc ccggcttccc
     6601 cacggatatg caggcgcagt ttatggcatt gaatgccgtg gcagagggaa gctgccgcgt
     6661 ggtggaaacg atttttgaaa accgctttat gcacgttccg gagttgaacc ggatgggggc
     6721 gaacattaca accgagggca atacggcatt tgtgcagggt gtggaacggc tttccggcgc
     6781 ggtcgtcaag gcgacggatt tgcgcgcgtc cgccagccta gttatcgccg gtttggcggc
     6841 gcgcggcgaa accgtggtcg aacggattta ccacttggat cgcggttatg aaaatattga
     6901 aaaaaaactc ggcagcgtcg gtgcgaacat cgagcgcgta tccggctgat cgggttgcga
     6961 tgccgtctga aggcttcagg cggcatcaat tgtttctgta tcgcaaacac tgattgaaaa
     7021 ggactgatta tggcattttt aaaactgacc gaacaaaacg tgcggggcaa aaccgtcctc
     7081 atccgcgccg atatgaacgt gccgttcaaa ggcggcaaaa tcagcgacga cacccgtatc
     7141 cgcgcctcgc tcgcgtccgt caaatactgc ttggacaacg gcgcgtccgt tattgtgatg
     7201 acccacttgg gccgcccgac cgaaggcgag ttccaccccg aagacgacgt tgcgcccgtt
     7261 gccgcgcact tgggcggcct gttgggcaaa gacgtgaaag tattgaacga ctggcgcgaa
     7321 aacaaaccgg ctttgaacgc gggcgatgtc gtgatgctgc aaaacgtgcg tatcaacaaa
     7381 ggcgagaaga aaaacgattt agaactgggc aaagcctacg ccgccttgtg cgacgtgttc
     7441 gtcaatgatg cgttcggcgc cgcacaccgc gcccaagctt cgaccgaagc cgttgcccaa
     7501 gccgcgcccg tcgcctgcgc cggcgtattg atggcgggcg aactcgacgc tttgggcaaa
     7561 gccctgaaac agcccgcgcg tccgatggtg gcgattgttg ccggcagcaa agtgtccacc
     7621 aagctgacca tcctcgaatc gttggcggac aaagtcgatc agcttatcgt cggcggcggc
     7681 atcgccaaca cattcctgtt ggcggaaggc aaagccatcg gtaaatcgct ggcggaacat
     7741 gatttggtgg aagaatccaa aaaaattatg gcgaaaatgg cgaccaaagg cggttctgtg
     7801 ccgctgccga ccgatgtcgt cgttgccaaa gcctttgccg ccgatgccga agcggtggta
     7861 aaagacatcg ccgacgttgc cgaagacgag atgattttgg acatcggccc gaaatccgcc
     7921 gccgcgcttg ccgatttgct taaagccgcc ggtacggtcg tttggaacgg gccggtcggc
     7981 gtgttcgagt tcgatcaatt cgcaggcggc acaaaagccc ttgccgaagc catcgcccaa
     8041 agcaaagcgt tctcgattgc gggcggcggc gacacgctgg cggcgattgc caaattcggc
     8101 gttaccgaac aaatcggcta catctccacc ggcggcggcg cgttccttga gttcttggaa
     8161 ggcaaagagc tgcctgccgt agccgctttg gaaaaaacgc ggcgagtaaa cggtttgata
     8221 taaaaaaatg ccgtctgaaa cgaaatggtt tcagacggca tttttgtgtg gcggttattg
     8281 tgctttggca gcccattccg ccggagtggc ggcaaccgaa atggaaagtg cgtgcagttc
     8341 gttgctttcc agttgggctt tgagtccgtc tttaatcagg cggtggcgcg cgaggcgtgc
     8401 cttgccttca aattctgatg aaacgatgac ggcgaaaaaa tggtgtccgt cgccttctac
     8461 ttcgatgtgt tcgcattttg ccacgcctgc aatcagggcc ttgacttgtt cgggtgtcag
     8521 catagtgggg agtccttatt ggggaaatgc gccattatac tggcaaatcg aaattccgtc
     8581 tgaaaacggc ttcagacgga atttttgacg gtgttttatt ttttggcttt gaagcagagc
     8641 aggtgttgtg tggtggcaag ccacgcgccg aatacgccca acgcgatgac gaagccgaac
     8701 accagcccga gttcgccgac gtagaagaaa cgccagccga tgttcaagcc gtagggtttg
     8761 aagatggcat cgaccaaggg gtgcacggca gagagcagcc aaccgcaaag ccctaagctg
     8821 acggcggcgg agaagatact ctgccacata gcctgataaa ggaaggggcg gcggataaac
     8881 gacgcgggcg cacccaggag tttggtgatt tcgatttctt ctttgcggct gaggatttgc
     8941 agtcggatgg tgttgtgtgc gacaaggacg aaagccatac ccagcgtcag ggaaagaaac
     9001 cataagattt tgcggatgaa ctcgttgatt tggtacagcg tttgcaccca ttcggtatcc
     9061 atagacgcgg attcgaccat aggcagcttg gtaatgtccc ggtagattgc ctgcatttgg
     9121 gcgggcgtgg ttgccgggtc gggggtaacg ataaagacat ccggcagggg gttgccgtca
     9181 agcatggaaa tcagattttg gtcgaggttg gactgtaatt ccgccaaacc gtcttccttg
     9241 ccgatgaagc ggatgttgtc gagccgttta tcgcgcgtca gcaggctgcg tacggtatcg
     9301 ctgtcgcttt gtgcggcggc ggtttccata tagacggtga tttgcggcga ctcgttgagt
     9361 ttgcccaaca cgctttgccc gctttggatg cccagataca taaacagcgg cagggtcatc
     9421 gcgacggcaa gcattatgag ggtaagcagt gtgccgaagg gttggcgcag aagctgtttg
     9481 agtgcggagc gcgcggattc gacgtgcagc gagaagtagt ggatgatgct catgcgagtc
     9541 gtcctttcga gaggcgcagg atgcggtgtc cgtagtccgc catcagggtt tcgtcatgtg
     9601 cggcgacgat gacggtggtt cccgcttcgt ggaaggtttt gaacaattcc ataatatcga
     9661 gcgcgtaggc acggtcgagg ttggcggagg gttcgtcggc aatcagcagg ccgggctggt
     9721 ggacgacggc gcgggcgatg cacaggcgtt gttgttcacc gcccgagagg gttacgggat
     9781 cgtccaattc tcgtcctttc aagccgactt tttcgatggc gatgcgggcg cgttcttcgg
     9841 ctttgcgcgg cggatagccg ataatccgca ggggtaggat gacgttttgc aggacattgc
     9901 ggtcgttgag gattttgtgg tcttggaaca cgatgccgat gtgttggcgc ataaagccga
     9961 tttggttgtc ggacaatgtg ccgaggtcct gcccgttaaa caggactttg cccatgctcg
    10021 gcttggtgat gcccgaaatc agcttgagga cggtggattt gcccgaaccg gagtgtcccg
    10081 cgataaaaat catctcgcct ttgttgattt ggaagctgac gtttttcagg gcttcaaaac
    10141 cgccgggata ggttttggaa acttgttcga aacggatcat aggaggtcct gtaaggaagg
    10201 gcggcatatc gtttcgggcg gttgcgtccg cagggatatg ccgtctgaac aataggtaag
    10261 acagtaccga ttataaagat tccgcctgtt tttagatagt ggataagttt ggcggatacc
    10321 gaaggccggg cgggaaaatg ccgtctgaag cggcttcaga cggcatccgg cgtttaacgg
    10381 cattttgaat gggcgagttt gacggcttcg gtcaggcttt tctcgttcac ctctcctgta
    10441 atggtctgcc tgtatccgca tttcggggct tcgacgacgg taaagggcag tacgccgaca
    10501 ttgtttccgt aggatttcat aaagtttcgg ctgttcgccc cggtgtaacg ccaaatcggg
    10561 tagctgaccg gggtctgctt gagaaagttg ccgatattgt cggatgtgtc gagcgcgatg
    10621 ccgaccatat cgacgctgcc ttttttctgc gctttgtacc atttggacat ggcgggcatc
    10681 tcttttcgac acggaccgca ccaagtcgcc cataggttgg cgatgcggac gggggctttg
    10741 agcgattgca ggttttgcgg ggtattgtct ttccatccgg ccagttcgtc tgccgaggcg
    10801 gtatgtgcgg caaatgtggc ggcaagggcg attgcggcga gattcaagcg tttcatcgtt
    10861 ttgtcctttg tatgtcgatt cgggtattgt aaaccgtttg gtacggttgg aggcggggta
    10921 ttgagtaaaa ccctgcgtaa gccgataata aggggattgt cgagaagtag ggaaatgtca
    10981 tgtctgaaat caaaattttc cacaatccgc gttgcagcaa atcgcgtgcc gccgtgtccc
    11041 tattggaaga acgcggcatt gctgccgaag cggtcaaata tttggatacg ccgcccgact
    11101 tgtccgaatt gaaggatatt ttcaacaaat tgggcttgga atcggcgcgc gggatgatgc
    11161 gcgtgaaaga tgatttgtac aaggaattgg gtttggacaa ccccgatttg gacaatgacg
    11221 cgctgctgcg tgccatcgcc gatcatcccg ccctgttgga gcgtccgatt gttttggcaa
    11281 acggtaaggc tgctgtcggc agaccgttgg aaaatatcga agcggtattg tgaacaaacg
    11341 gctgttttgc agccgcaacg gcttgcgcta ttacctgttg ggcggtttct gtttgagtgt
    11401 ttttccgctt ttgctggtgt ttgcctcatc cgtttgggcg gtttatcgga cgggcgggca
    11461 ggttttgccg ccgtatgtcc gggcggatgc cgcgctggtg ttgggcgcag ccgcgtggga
    11521 caaacgccct tccccggttt ttcgggaacg catcaaccac gccatcgcgc tttaccaaag
    11581 ccgcagggta ggcaaaatcg tgtttacggg agggcgaacc aaaaaaggct atatgaccga
    11641 agccgaggtc gggcggcggt acgcgctcaa acagggggtt ccggcgcgca acatcctgtt
    11701 tgaaaacact tcgcgcaaca cttatgaaaa cctgaacaac atccgtccgg ttttgcgtgc
    11761 caacggtatt gccagcgtgg tgattgtcag cgatccctac catttggcgc gggcggcgga
    11821 aatggcggag gatttgggtg tccgcgttta tatgtctgcc acgccgacca cgcggtttga
    11881 tgcgggaaat aagaaaaaaa tctttatgct gcaagagggg tatgcgttat ccctctaccg
    11941 tttggaaaaa tggggcagcc gctttttggg atggctgtct gattgaagcg gtagaaaatg
    12001 ccgtctgaaa cttatccgca ggcagggttc ggacggcatt tttgatgcct aagcacggta
    12061 aaaacagtaa aataaacgct ttcaaagatt tcggagccat tatgaccgtc aaaacccgtt
    12121 tcgcccccag ccccaccggt tacctgcaca tcggcggcgt tcgcaccgcc ttgttttcgt
    12181 gggcgtttgc ccgccatcat aaaggcgaat ttttattgcg tatcgaagac accgacttgg
    12241 cacgttccac cgccgaatcc gtcaacatca tccttgacgg catgaaatgg gtcggtctcg
    12301 attacgacaa cgccgacaac gtggtgtacc aaacccgccg tttcgaccgt tataaagaag
    12361 tcattgccga actcttggca aaaggcgatg cctactactg ctattgtagc aaagaagaat
    12421 tggaagcgat gcgcgagaaa gccgaaaaag aaggcacggc gacttacgac cgccgctggc
    12481 ggccggaagc aggcaaaacc ctgcccgaaa ttcctgcagg cgtgcaaccc gtcgtccgct
    12541 tcaaaacgcc tttggacggc gttaccaaat ggacagactt ggtcaaaggc gaaatctcca
    12601 tccccaacga agccctagac gacctgatta ttgcccgcgc cgacggtacg ccgacctaca
    12661 acttctgcgc cgtggtagac gactacgaca tgggcgttac ccacattatc cgcggggacg
    12721 accatgtgaa caacaccccg aaacaaatca acatcttgaa agccatcggc gcgaacctgc
    12781 ccgaatacgg gcatctgccg atgattctca acgaacaagg caaaaaaatc tccaaacgca
    12841 gcggcgatac cgtcgccatt accgatttcg gcgcaatggg cattctgccc gaagcgatgc
    12901 tcaactattt ggcgcgtttg ggctgggcgc acggcgacga tgagtttttc acgacggaac
    12961 aattcatcga atggtttgat ttgaaagacg tttccccgtc cccaagccgt atggatttga
    13021 aaaagctcta ttggatcaac ggcgaacaca tcaaaatcac acccgacggc aaacttaccg
    13081 aactcgtcaa accgcgcctt gcgctgcgcg atattcatga aaccgaaaaa cctgcgttgg
    13141 aagacgtgtt ggcactggtc aaagaccgcg cccaagattt gaacgccttg gcagacgaat
    13201 gcctgtattt ctacaaaaaa caagtccctg ccgaagccga tgttgccaaa cactgggacg
    13261 acgaagccgc cgcccgtatg ctgcgcttcg ccgaacgcct cgaagggctg gaagactgga
    13321 acgccaaagc cattcacgac ctcttcaaac ctttctgcga cgaagaaggc atcaaaatgg
    13381 gcaaactcgg tatgcccctg cgccttgctg tctgcggtac ggcaaaaacc ccgagcgtcg
    13441 atgccgtgtt ggcattaatc agcaaagagg aagtgttgaa acgcatccgt gcttaagtta
    13501 tccacaggtg cgggcaatgc cgtctgatgt cggaatgcgg cttcagacgg cattcagacg
    13561 gcattcagac ggaagttgaa catgaaaaaa atattgtatt ttctgatgtt tgttttttct
    13621 acaagcgtat gggcaggggg tgcggaagac aatttgctca gcatccaatc cggttaccgt
    13681 gctttactgc aaaagcaaaa caatctggac ggaaaaatca tcgggatgca gtcggatttg
    13741 gaagatgcgc gccggcgttt gcaggcggct caggcagaca tcgcccgttt ggaagcggaa
    13801 attcctgcag caatggccca aaaagcccgg caggctgaag agatgaggca aatcggagtg
    13861 cgtttggacc atgcttggaa tgcggtttac ggcgcagggg aacgaaagcg tcgggggaat
    13921 tgaccgatga attccctctt tgtggacaac actgttttta ttacacggct gaaagccggg
    13981 catatcggca ggttggttca ggcgttgttt gaggagtggc acggatttga accgtggtct
    14041 tctgtggata agattcgtgc ctattacggc agatgtttga aggatgacga actgccgctg
    14101 gcttttgcgg ctgtggatga ttccggaacc ctgttgggtt cggctgcagt caagcggcat
    14161 gatatggaaa attttccaca gtatgaatat tggttgggcg atgtctttgt tttacctgaa
    14221 tatcgcggaa aaggcattgg caggaggctg gtcgcccact gcataggcgc agcgcgttcg
    14281 ctggggataa agttcttgta tctttatacg cctgatgtgc aaatatttta tgaatcattc
    14341 ggctgggcgg ttgtcgggcg acatttccat aacggggaat gggttacggt tatgcgtttg
    14401 gatgtggata aggtttaagc cgaatgggca gatgccgtct gagctgttcg gacggcattt
    14461 tttatgtttg gaaatttcct gtcagatata gtggattaac aaaaaccggt acggcgttgc
    14521 cccgccccgg ctcaaaggga acgattccct aaggcgccca agcaccgggc gaaccggttc
    14581 cgtaccattt gtactgcctg cggcccgccg ccttgtcctg atttttgtta atccgccata
    14641 aagtttatcc acagattttt ccatatggtt tgatgctgct ccgccaacgg aaattaagat
    14701 acggatttgc acaaatcttt cagacggtac agttctgaca atgcttcgcg cggggtcagg
    14761 ctgtcgggat cgaggttttc cagggctgct gccaatatac cttcaaaatg tttttcctct
    14821 gctttatcca caaagcagtc cacattcggt tcatctcctt tttcagacgg catggtactg
    14881 aaaatatcca gttggggacg gtttgcggcg gcttggtttt ccagtccgtt caaatgcttt
    14941 tgggcggctt tcaatgcgcg cacaggcagg ccggcgagtt tggcgacggc gatgccgtag
    15001 ctttttccgg cgggaccggg ttggatttgg tgcaggaaca cgatgtcccg tccctgttcg
    15061 agcgcggaaa ggtgcatatt gacggcggcg gcgtgggctt cgggcaggta ggtcagctcg
    15121 aaatagtggg tggcaaacag gctgaaggat ttgttttttt gcagcaggtg ttcggcgatt
    15181 gcgtgcgcga gggcgaggcc gtcgaaagtg gaagtaccac gtccgacttc gtccattaaa
    15241 acaatgcttt gttcggtggc gtgatgcagg atgtaggcgg tttcgctcat ttcgaccatg
    15301 aaggtggagc ggttggaggc gaggtcgtcc gatgcgccga tgcgggtgaa gatttgatcg
    15361 acaggcccga ttgtggcggc atcggcaggc acgaaacagc cggtgtgtgc caataaaaca
    15421 atcagcgcga cttggcgcat ataggtggat ttgccgccca tattggggcc ggtgagcagc
    15481 atgaggcggt gtttgtggtc gaggtcggtg tggttggctg tgaagtggcg tacctgctgt
    15541 tcgacaacgg gatggcggcc gttttcgatg tggacaaccg gatagtcggc aaactcggga
    15601 cggacaaagt tccgctcttt tgccaaggct gaaaatgtgg acaacacgtc cagcgcggcg
    15661 gcggctttgg cggctttttg aagctgcggc aatgccgtcc gaaggttttt caatacgccg
    15721 tcaaagagtt gtttttctaa ggcgagggct tggtcttgag cagtcagcac tttgtcttca
    15781 aaggctttca gttccggcgt gatgaagcgt tcggcgtttt tgagggtttg ccggcgttgg
    15841 tagtcggcag gtgcttgttc ggcttgggtt ttggacaatt caatgtaaaa gccgtgaacg
    15901 cggttgaact cgactttgag tgtggacaaa ccggtacgtt cgcgttcctt ggcttccaaa
    15961 tccagcaaaa attcgtcgcc atggttttga atgcggcgca attcgtccag ttcgggatga
    16021 aaaccgtggt tgatgacatt gccgtctttc agccagacgg aaggttcggg catcacggcg
    16081 gctttgaggg tttcggcaac tggcagggtt tcggggaaaa cggctttaag ggtttccaac
    16141 aggctgctgc cggttgcgga caagtcgatt tgtgccaact cgaacagact gtcgcgtaaa
    16201 gatgccaaat cgcgcgggcg ggcattgccg acggcgatgc gggcggcgat acgttcgatg
    16261 tcggcaatgc tcttcagatg gcattggagg ggttcgtatt ggctttccag cgcggttacg
    16321 gcttcttggc gcgctcggat gtgggcgcgg ttgcgtaaag ggtggtgaag ccacaatgcc
    16381 aacaggcggc tgcccatgtg ggtggcgcag ccgtcgagta tggaaaacag ggtcggcgtt
    16441 tttttaccgg agagggtttg cgtgatttcg agattgcggc gcgtggcggc atccatgccg
    16501 atatattggc tgtcggtttc gagcgacagg ccgtccaaat gttgcggcat caggttttgc
    16561 gtcaggcgga tatagttcaa cagcgcacct gccgcgccaa tcgaaacggc gtgttctttg
    16621 ctgtccaaac cgaagccgcg caaatcttgg cagccgaaat attccgtcag cagtttttcc
    16681 cccgcgtcgg cggcaaactg ccacgcgttc aggcgtgtaa cacccgatgc cgtctgaagt
    16741 tgcggtgcgt ttttactgtc cggcagcaaa atttccgccg cctgcaggcg cgccagttcg
    16801 tcgttcaatt tatccgcagt tgtcagcttg gttttgaatt cgccgctttg cagcgatgcc
    16861 cacgccaaac cgatgtattt tttgtcggga gacacggcaa cgatgcggtt ggtttccttg
    16921 tcttccagta atgcggaatc ggttagcgtg ccgggcgtta cgatgcgcac gactttgcgc
    16981 tccaccggcc ctttgcccgc gccgacttcg ccgacctgtt cgcaaatcgc cacgcttttg
    17041 cccaacttga ccaggcgcgc cagatactgt tccgccgcgt gaaacggaac gcctgccatt
    17101 ttaatcggca cgccgtccat ctgcccgcgt gtggtcaggg tgatgtccaa caatttcgcc
    17161 gcttccaccg cgtcatccaa aaacagctcg taaaaatcac ccatacggta aaacactagt
    17221 ttgtcggtat gttgcgcttt tatgccgagg tattgctgca tcattgggga aacggcggat
    17281 ttggacatgg ggctgcctta ttattaggaa aacggtattg tagcaaaagg ttttgtcttc
    17341 gacggaatga gactgaccgt aagcctgtca ataaagttgt ggagacaaat aggaaaacgg
    17401 caaagttatc cataagaata ttattaagtc aaaaaacatc cacgcttatc tgtcttatac
    17461 ggcaagcctt aaactcaaat ttatttaaca aaaaggtaat gaaaatattg gatatttgct
    17521 acttatacac aagcaggtcg gcaatcatca aaatcatcat taaaatttta aaatcaaata
    17581 ttgaatgcgc gtttttgaaa tagcgaaatt cacctgcgtc aaacaaaacg caagtaattt
    17641 tatgtaatag tggaatgtag ttttatttca gaatacaata caggcatcgg actttatttc
    17701 acaaccggga gacaaaatat gagcatcaaa gtagcgatta acggtttcgg ccgcatcggc
    17761 cgcctcgcat tgcgtcaaat tgaaaaagcc cacggcatcg aagtcgccgc cgtcaacgac
    17821 ctgacccctg ccgaaatgct gctgcacctt ttcaaatacg acagcacaca aggccgtttc
    17881 caaggtactg ccgaattaaa agacgatgcc atcgtggtaa acggcagaga aatcaaagtt
    17941 ttcgccaatc cgaatcccga agaattgcct tggggcgagt tgggtgtgga tgtcgtcctc
    18001 gaatgtaccg gtttctttac caacaaaacc aaagccgaag cacatatccg tgccggcgcg
    18061 cgcaaagtcg tgatttccgc acccggcggc aatgatgtga aaaccgtcgt atatggcgta
    18121 aaccaagaca tcttggacgg tagcgaaact gtcatttccg ccgcttcctg taccaccaac
    18181 tgccttgccc cgatggcggc agtcctgcaa aaagagttcg gcgtagtcga aggcctgatg
    18241 accaccatcc acgcctacac cggcgaccaa aacacccttg acgcgccgca ccgcaaaggc
    18301 gatttgcgcc gcgcccgcgc cgccgcgctc aacatcgtgc ccaacagcac cggcgccgcc
    18361 aaagccatcg gtctggttat ccccgaattg aacggcaaac tcgacggctc cgcccaacgc
    18421 gtccctgttg ccaccggctc gctgaccgaa ttggtttccg tcctcgaacg ccctgcaacc
    18481 aaagaagaaa tcaacgccgc gatgaaagcc gcctccagcg aatcttacgg ctacaacgaa
    18541 gatcaaatcg tttcttccga cgttgtcggc atcgaatacg gctcgctctt tgacgcaacc
    18601 caaacccgcg tgatgaccgt gggcggcaaa caactggtga aaaccgttgc ctggtacgac
    18661 aacgaaatgt cctacacctg ccaactcgtc cgtactttgg aatacttcgc aggcaaaatc
    18721 taaggcttga acaaacctgt ggataagtaa atgccgtctg aacacattgc gttcagacgg
    18781 cattgtttat ctgtaacttg atattgcggc ttatggcgaa caaacaacag ccgtttgttc
    18841 aactgctatg cggcaaaacc ggattttgcc cgatttaaag cggggttggg gaaagattaa
    18901 taaccgtcgc gcggttttcc tcgatttgaa acctgacttc gtaatccgca atattcatca
    18961 cataaatccg ttcgggaata ttctgatagg cggggcgcgg atcttgggca atgctttggc
    19021 tgatgaggtt tttggtgttt gcagataaat tttccgcgcc gatgttttcc tgccaaacga
    19081 cttccaactc tacgggtttg ccgctgacga aaccggatgc ggcatcgggt ttggattcga
    19141 taaaggggat ataaggcttg atgtccacaa tcggcgtgcc gtccagcagg tctgagccgc
    19201 tgcaatagag gcggacgggt ttgccggttt cgatgcgttc gagtttaagg agagagagtc
    19261 cgagatggtt ggggcggtgg gggctgcgcg tggcgaacac gcccattttt tgtttgccgc
    19321 cgagccgtgg cgggcgcacc atttgcgccc aaccttcatc caatacgccg tggaagataa
    19381 aacttatcca cacataatcg aaatcttcca acccgcgcac gctgtctgcg gtaaattcgg
    19441 gattcagctc gatgcaggct tctgcggcgg agaccaaacc gggctggcgg gcgatgccga
    19501 atttctgttt gtagggcgag cgggcggtgc cgatgggggt aatggtataa gtcataagat
    19561 tgtggataag ttttttggaa aaaccttatc ttatcacagc cggttttacc gctttcgggt
    19621 agtaatttgt tataatacag ggcatttaaa tcgcgttgaa ataaagtagc acagaatcct
    19681 tgtgtccaag acgctcttaa aaaggcattg gagtattgat aaaaaagaag cataacggtt
    19741 cgttataacg aagagggaat cccgtatatt acacccaagt caggttgtta tctttccttt
    19801 tggatctcac ttacaggagg tgagatctga aattttgggt aataaaatcg gattacagct
    19861 tctagcaagg agtaccttag gtatagctaa aatggagggg gtggatatga catatcatat
    19921 ccacctagat ggattgtgct ccttaaataa agaaggtaag agttttgtta ttcgtaaaat
    19981 agagaatgac gatcttaagt gaagaagtaa tgagatgatt gtttccattg atgttgatgc
    20041 acaaaaaacg tttacgccac tgtgtcctga cgaactgccc gtgaacgagg ggcatttgat
    20101 tgtcgaggag ttaaatgccc aagccgcttt ggcggatttg cgtgtgatga cgaaagatgc
    20161 gcatcatatg gcggcgaaat ggcttgtgga taaccctgtt gatatgttga agccgacagg
    20221 tttgtccgat gcggatttga cttgggtggc tcatgcgatg gtcggtacgt gcggctatga
    20281 attgttggac ggtctgcctt ccgccaagga atacgattat tgcgtttgga aaggtgtcga
    20341 tcccgaattg catccttacg gcgcgtgttt tcacgatatt gaggaaaaac tgagcactgg
    20401 attgattgag tggctgcgtt gtcaaaatac gaatatggtc attgtcggcg gcttggctac
    20461 ggattattgt gttaaaacaa cggttttgca gttacttaaa ggcggtcgtt ggcaggttat
    20521 cgtgaatgaa gcggcttgtc ggggtattgc gcccgatacc attgaagcag cgtggcagga
    20581 aatgcgttct tcgggtgcga taattttgaa aaacgccgaa aaaattaaaa aatatattaa
    20641 taataaataa cttataattg tttcacgtga aacaccctgt ctacatatga aaattttact
    20701 tgtccgtttg tccagtatgg gtgatttaat tcacactttg cccgcaatcg aagatttggc
    20761 gcgacaatgc tctgatgtag aactgcattg gctatgtgaa gccgggtttg cgggtattgc
    20821 acggttgcat ccgtttgtaa aaaaggtcca tgtaatgaaa tggcggcaat ggcgcaaaca
    20881 tctctttcag gctgaaactt ggcgggagat gggtcgtttg aaacaggctt tgctgcagga
    20941 agcatttgat tttgtattgg acagtcaggg tctgattaaa agcgcgtgtt tcgccaaaat
    21001 ggcaaaatcc cctatttatg gtttggataa acacagtgcg cgtgaggggg tggctgcttt
    21061 ggcgtatgat aaaaagtatg tcgtaccgaa aggtagaaat gccgtttggc gcaaccgtga
    21121 tctgtttgcc caggtgtttg ggtatgcaat gcctgagacg caggtatttg gtttgtccgt
    21181 tcctgaagca agccgtctga aaaatttagc gcagccgtat tatgcggctt tgcacgcgac
    21241 cagtcgggac agtaagttat ggcctatgga aaattggcgg gagttgctgc agaaactgaa
    21301 tcaaaaacaa caatgcaatg tttatctgcc ttggggaaac gaggctgaaa aagtgcgtgc
    21361 ggaacagatt gcagacgggc tgccgtttac catagtgtgc gctaaaatga atttattaca
    21421 ggcggcgtat ctgttgaaac acgcggttgg aatagttggt gtagataccg gtttgctgca
    21481 tttggcaaat gccttggaaa aacctgtggt cggcatttat accgataccg atccgattaa
    21541 aacaggcgtt caagtttcgg ctattgcaaa aaatgtgggc aatatcgggc agattccgac
    21601 cgcagatttg gtttatcaaa cgctgatgga ttgtgtttca gcagataaag gctaaagggt
    21661 cgtctaaaac tgatatggcg gcatgatatt ttccaattta ttagatacag gaatgtatat
    21721 ccgccagcct gagtaaatac tttaaaaatg gtataatcgg taaaattatc cgaaagattg
    21781 ctaaataaat tcgtttttaa ggaaaacgga tttgatttta taaagaagaa ggtttggttt
    21841 cgctggatgg tgaaagcagc gttcttttta ggagaattca atgaaagcac tggtcgcagt
    21901 aaagcgcgta gtggactaca acgtcaaagt ccgtgtgaaa gccgatggtt cggatgtgga
    21961 catcggcaat gtcaaaatgt cgatgaaccc gtttgatgaa atcgctgtgg aagaagccgt
    22021 ccgtttgaaa gaagccggga aagtaagcga aattgtagcg gtttctttgg gtgaaaagaa
    22081 atgcgaagaa accttgcgta ccgctttggc aatgggtgcc gaccgtgcca ttcatattga
    22141 aaccgatgtg aaattagagc cgctggcagt tgccaaactg ctgaaagctg ttgcggacaa
    22201 agaaaatccg caaattttct ttttgggcaa acaagcgatt gatgacgatg ccaatcaagt
    22261 ggcgcaaatg ctggcagctt tgttgaatgc ggcgcaaggt acatttgttt caaaagtaca
    22321 aattgaaggt gatgaagtac agattgtgcg cgaaattgat ggcggcgaag aaacgattgc
    22381 gttgaaactg cctgcagtca tcagcgcaga tttgcgtttg aacgagccgc gctttgtcaa
    22441 actccccaat attatggcgg caaagaaaaa acctctggaa aaactggatt ctgccgattt
    22501 ggctaccgat atttcgcctc gtctgaaaac ggtgaaattt gccgaaccta aggcgcgtca
    22561 ggcaggcgta aaagtagcaa gcgttgccga attggttgaa aaattgaaaa acgaagccaa
    22621 agtgatttga ggaggctgaa atgagcgtat tgattattgc cgaacacgac aacaaacagt
    22681 tgaatcctgc cactttgcat gctgttgccg ctgccgccaa actgggcaag gtcgatttat
    22741 tggttgtcgg aaacggcgca tcgtccgtcg tggaattcgc gaagcaagta gcgggagtgg
    22801 aaaaagtttt ggttgcagat gctgctcatt atgctgaagg tttggctgaa gagttggctc
    22861 cgctggttgt caaattggcg gcggattacc gctatgttgc agcaacggca accacattcg
    22921 gtaaaaacct tttgccccgc gtagccgcct tattagacgt accgcaaatt tctgatttga
    22981 ccgagattgt agataacacg acttttgtac gtccgattta tgcaggtaat gcatttgaaa
    23041 ccgtgcaagc cgattcagaa aaattggtgc tgaccttccg tgtgacggct tttgacgcag
    23101 tggcggcgca aggtggtaat gctgaggtaa ttaatgttaa aacaacccct gcccaaaacc
    23161 tgagtcgttt tgttaaccgt caactttccc attccgatcg tcccgaactg actcaggcaa
    23221 aagtgattgt ttccggtggt cgtgcgttgg gtagtgtgga aaaattcagt gaagtgctga
    23281 caccgttggc ggatgtttta ggtgcggcaa tcggtgcatc ccgtgcggcg gttgatgccg
    23341 agtatgcacc aaacgatgct caagtcggac aaaccggtaa ggtggttgcg ccgcagctct
    23401 attttgcaat cggtatttca ggtgcgattc aacacgttgc cggtatgcag gacagtaagg
    23461 tgattgttgc aatcaacaaa gatgccgatg cgccgatttt caatgtagcc gattacggat
    23521 tggttggcga tttatttgaa attgttccac aattaataga attattgaaa aattgatgtt
    23581 tcacgtgaaa ctttcaggcg actttgttat taatgaggtc gtctaaaaac atttcagcat
    23641 gattacgact tataaaacca ttacttcccc gacgcaggct gagtttaaag ataaaggcag
    23701 ccgttttatt gcatttgcct atccgattcg gacattggct gatgtgaaaa aatacctcaa
    23761 tctgttaaag gaagagcatt ataaagcgcg gcattggtgc tatgcctatc gtttaggtgt
    23821 gaacgggatg cagtttcgcg gcaacgatga tggagagcca ccaggaagtg ctgggcggcc
    23881 aattttggga cagattgatt cggtgggtat cactgatgtt ttggtcgtgg tcgtccgcta
    23941 tttcggtggt gctttattgg gcgttcccgg cttgattcac gcatacaaag aggcaacggc
    24001 tcaggcgttg gcgatcgcgg aagtggttga aaagaatatt gaaaaaaccg tttggctgaa
    24061 atgtgagtat ccgtttttga atgaagcaat atgcattgcc aaacaatatc aggcggatat
    24121 attggagcag gatttgcagt tggattgcct attgacggta agtttgtcgt tggcaaatta
    24181 tgaggcctgt gttgcggctt ggaagaatac acggcaggtt gaagtaaata cggaaaagcc
    24241 ttttgaatga aaggttgtat gaaatgcttt gtaatggtat ttcaggcaac ctcttcgctg
    24301 attaattttc ttgtgtatat ctgtctaagg tatcgacgct ggagcataaa tatagccaag
    24361 ccaattgatg gaattcgccc ggcgcgttcc ataatgcatc ttcactcata atgctatatt
    24421 cgccttctgt ttgcaaattc agttctaccc cgtcttcaat agtctgatgg cgcacctgat
    24481 actcgtgggt atagaaattg tccatatcgc gaattaaagc gacggcatat ttccaacggc
    24541 ggatgttttc ttccagttgc agtagtaaat gacaccattc gcggtatttg ctttggattt
    24601 catcaatacg tttttgcgtg tttgcttgtt attttttaat ttgtgaataa gttgaatagg
    24661 ttgcagagtg gaatgaagtg gtcttttgtt tgattctact aaaatttctt cagatgggac
    24721 aaaagttcct ttgtatcgag ttttcgttaa aagtacatat ttgtcaagga gatggcatga
    24781 aactgctggt tatcggtaat ggcggtcgcg aacacgcgct ggcttggaag ttggcgcagt
    24841 cgcccaaagt ggaaacagta tttgttgcgc ccggtaatgc cggtacggcg attgaatcca
    24901 agctccaaaa cattgccttg accgcgtatc aggatttgat tgaattctgc cgtaaagaaa
    24961 atattgtttt taccgtcgtc ggccctgaag cgcctttggc ggcaggtatt gtggatgatt
    25021 ttcgtgccgc agggctgaaa atatttggtc cgacacaata tgcggcgcag ttggaaagtt
    25081 ctaaagattt cgccaaagca tttatggtga aatacaatat tccgaccgcg caataccaaa
    25141 cctttgaaaa tgccgatgcc gcacatgatt acgtcaatca gaaaggtgcg cctatcgtta
    25201 tcaaagccga tggtttggtg gcaggtaaag gcgtgattgt ggcaatgact ttggatgaag
    25261 cgcatgctgc gattgacgat atgttgttgg gcaataaaat gggcaatgcc ggcgaacgcg
    25321 ttgtgattga agattttctg caaggcgaag aagcgagctt tatcgttatg gttgatggca
    25381 atcatgtatt acctatggcg accagtcgag accacaaacg tcttttagac ggagataaag
    25441 gcccgaatac gggcggtatg ggtgcgtaca gtcccgcgcc tgtggtaacg cctgctgtgt
    25501 acgagcgtgc gatgaacgag attattttgc cgaccgtagc gggtatgaaa gcggaggggc
    25561 acgagttcac cggctttttg tacgcaggtt tgatgatcga tcaaagcggt gcaccctata
    25621 cgattgagtt taactgccgt ttcggcgatc ccgaaaccca gccgattatg agccgtctaa
    25681 acagcgattt ggcggatttg gttgaagcgg caatagacgg cagactggac agcgtcaaag
    25741 cggaatggaa tccgcaaact gccgtgggtg tggtactggc ggcgcaaaat taccccgaaa
    25801 cacccaagaa aggcgatgtt atttccggtt tggatgacgt caaccgaatc ggcaaagtat
    25861 tccatgccgg cacaacggta aacgagaaag gcgacgtgtt gaccaacggc gggcgcatat
    25921 tgtgtgttgt aggattgggc gacgatgtgg cgcaagcgaa agccaaggcg tatggcgcgt
    25981 tggaaaaaat cagcttcgac gggatgcaat accgtaaaga cattgccgat aaagcgatta
    26041 accgttaatt agaaagagga tgatgccgtc tgaagccaag tatgactgat gcgcttcaga
    26101 cggcatttta tattatgtta ttttcgagaa ttatcgcagc atcagcgcaa cgtaagttaa
    26161 gcgtttattt gaaaaaaggc ggtttggttg cttatccgac cgaatcttgt tatggcttgg
    26221 ggtgtcttcc gacattagcc aaagcactcg gtaagttggc gcatttgaaa aaaaggccgc
    26281 aacacaaggg tatgattgtc atcgggaatc aacttgaaca attgcagcct ttacttcaaa
    26341 tgccgtttga aaacattcaa accatgctta gaaacgaatg gcctgctcct aagactttcc
    26401 tattgttggc taaatctggc gtattacctg cgttgcgcgg gaagaggcgc agcaagcttg
    26461 cggtcagagt gcccgatcat acaggtgcaa ggcgtttgtg tcaggcattg ggtatgcctt
    26521 tggtttccac ttcatgtaat cgggcgggta aacgcgcgtg tcggacggag agggaagtca
    26581 gaaggcagtt cggacgcgat gtgtggattg tcggtgggag aatcggcagg cagaaatcgc
    26641 cgagtcagat tatcgatggg gaaacaggta aacgtttgcg ttagatatgg atgcttgagt
    26701 gaaaactttt ttgcatacac aaagaaaaaa ttttttggat ggtatttctc tacatgattg
    26761 ttatcgcacg gcgataaagg tagaaggacg caacgtctgc tttgattttg aggacggttt
    26821 tgtcgttctg gacaataatc cgaacaatca agcgggcaag catctgaaaa cagatttttc
    26881 ccgtgttgtt ttaacccatg aaaatagaaa cgccgaagac gactatttgg tcgatatttt
    26941 tgaagacatc gtctttttag ggaaacgttt gtttacagtc agaaaatttc ttgatttttc
    27001 tgaattgctt gaaatgatca atgcacaagg ctattttctt gaatttctgt atctgtatga
    27061 atgtatcggt gtcaaaacaa gcgattattt tttagaggct gtgttggtta cgggacggag
    27121 ccgggaatgt aaaaaatgct ttatcaaaat tatgagggca agcagttttg tgtatcaatg
    27181 gaacaattta cgtttgaata gtgagattgt atagcgggtt tatatttcgg atggaagccg
    27241 gtttaaaaaa ataatgtgga taatttcagg gaatggcaaa atgtcgtctg aaatgctgat
    27301 atttcagtat tccagacggc atttttataa taaggcttat ggataaattt gtgtataagt
    27361 tgaaatcaga gttttttccc gcatacggtt tcatattgtt tttcagttaa tgtcccggac
    27421 aggatgctca tcgggcggag ggaagaggct gtgtagtttt gtgtggaaat ttccgtgttt
    27481 tttgtatcaa ataactgtag cgaacttaag cggtatgttt tgttgttgca gtggatttcc
    27541 cactcggcaa tggcagtctt gtatgcgggg gtgttggcaa aacgttcttg tttcagattg
    27601 gtaacaactt ttttatcttg gaaaatcatc agatttccgt tttttctcac actgtcttta
    27661 ttaatatatg tatgaatatt gccgtttgag attgtgccga tattttccca tcgggcagac
    27721 ttgtccggtg tgctgcaggc ggttatcaaa atagggagta aaacgaaaga tag
//
LOCUS       DAXFWM010000020        27176 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000020 DAXFWM010000000
VERSION     DAXFWM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27176
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.020"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(119..982)
                     /gene="rfbD"
                     /locus_tag="ACFI8B_000852"
     CDS             complement(119..982)
                     /gene="rfbD"
                     /locus_tag="ACFI8B_000852"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC3834117.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPHQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            1175..2038
                     /locus_tag="ACFI8B_000853"
     CDS             1175..2038
                     /locus_tag="ACFI8B_000853"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC3834118.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            2272..4395
                     /gene="pnp"
                     /locus_tag="ACFI8B_000854"
     CDS             2272..4395
                     /gene="pnp"
                     /locus_tag="ACFI8B_000854"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC3834119.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(4468..5136)
                     /locus_tag="ACFI8B_000855"
     CDS             complement(4468..5136)
                     /locus_tag="ACFI8B_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC3834120.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(5133..5447)
                     /locus_tag="ACFI8B_000856"
     CDS             complement(5133..5447)
                     /locus_tag="ACFI8B_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834121.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            5584..6435
                     /gene="dapF"
                     /locus_tag="ACFI8B_000857"
     CDS             5584..6435
                     /gene="dapF"
                     /locus_tag="ACFI8B_000857"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC3834122.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVLIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            6432..7037
                     /locus_tag="ACFI8B_000858"
     CDS             6432..7037
                     /locus_tag="ACFI8B_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834123.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            7233..8165
                     /gene="cysK"
                     /locus_tag="ACFI8B_000859"
     CDS             7233..8165
                     /gene="cysK"
                     /locus_tag="ACFI8B_000859"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC3834124.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            8474..9382
                     /locus_tag="ACFI8B_000860"
                     /pseudo
     CDS             8474..9382
                     /locus_tag="ACFI8B_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            9428..9577
                     /locus_tag="ACFI8B_000861"
     CDS             9428..9577
                     /locus_tag="ACFI8B_000861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834125.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            9801..9908
                     /locus_tag="ACFI8B_000862"
                     /pseudo
     CDS             9801..9908
                     /locus_tag="ACFI8B_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10013..11032)
                     /gene="lepB"
                     /locus_tag="ACFI8B_000863"
     CDS             complement(10013..11032)
                     /gene="lepB"
                     /locus_tag="ACFI8B_000863"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC3834126.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRAPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(11178..12971)
                     /gene="lepA"
                     /locus_tag="ACFI8B_000864"
     CDS             complement(11178..12971)
                     /gene="lepA"
                     /locus_tag="ACFI8B_000864"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC3834127.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(13113..13814)
                     /locus_tag="ACFI8B_000865"
     CDS             complement(13113..13814)
                     /locus_tag="ACFI8B_000865"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC3834128.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RIVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            13982..15112
                     /locus_tag="ACFI8B_000866"
     CDS             13982..15112
                     /locus_tag="ACFI8B_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC3834129.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            15146..16123
                     /locus_tag="ACFI8B_000867"
     CDS             15146..16123
                     /locus_tag="ACFI8B_000867"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC3834130.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            16127..16477
                     /locus_tag="ACFI8B_000868"
     CDS             16127..16477
                     /locus_tag="ACFI8B_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC3834131.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            16482..17261
                     /locus_tag="ACFI8B_000869"
     CDS             16482..17261
                     /locus_tag="ACFI8B_000869"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC3834132.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFVVAEAHEHIYCTIGVHPDSKEAEEFSIAEMAEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            17310..17501
                     /locus_tag="ACFI8B_000870"
     CDS             17310..17501
                     /locus_tag="ACFI8B_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC3834133.1"
                     /translation="MSALLPAACQHVSDRHGKFRFQFCIAKGGRREVKKDESGGGYAL
                     CHLPDSRIVEEWEYCRSQH"
     gene            17602..17913
                     /gene="grxD"
                     /locus_tag="ACFI8B_000871"
     CDS             17602..17913
                     /gene="grxD"
                     /locus_tag="ACFI8B_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC3834134.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            18019..18645
                     /gene="upp"
                     /locus_tag="ACFI8B_000872"
     CDS             18019..18645
                     /gene="upp"
                     /locus_tag="ACFI8B_000872"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC3834135.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            18668..18988
                     /locus_tag="ACFI8B_000873"
     CDS             18668..18988
                     /locus_tag="ACFI8B_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC3834136.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            19157..19798
                     /locus_tag="ACFI8B_000874"
                     /pseudo
     CDS             19157..19798
                     /locus_tag="ACFI8B_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            19828..20652
                     /locus_tag="ACFI8B_000875"
     CDS             19828..20652
                     /locus_tag="ACFI8B_000875"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC3834137.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            20652..21620
                     /locus_tag="ACFI8B_000876"
     CDS             20652..21620
                     /locus_tag="ACFI8B_000876"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3834138.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKESLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            21902..22324
                     /locus_tag="ACFI8B_000877"
     CDS             21902..22324
                     /locus_tag="ACFI8B_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC3834139.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            22342..23106
                     /locus_tag="ACFI8B_000878"
     CDS             22342..23106
                     /locus_tag="ACFI8B_000878"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC3834140.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            23118..24452
                     /locus_tag="ACFI8B_000879"
     CDS             23118..24452
                     /locus_tag="ACFI8B_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC3834141.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            24449..25666
                     /locus_tag="ACFI8B_000880"
     CDS             24449..25666
                     /locus_tag="ACFI8B_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC3834142.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVFVVVGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPENMRRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            25741..26805
                     /gene="hemE"
                     /locus_tag="ACFI8B_000881"
     CDS             25741..26805
                     /gene="hemE"
                     /locus_tag="ACFI8B_000881"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC3834143.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(26868..>27176)
                     /locus_tag="ACFI8B_000882"
     CDS             complement(26868..>27176)
                     /locus_tag="ACFI8B_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3834144.1"
                     /translation="KLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGRKLSRA
                     TGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLVYQSAK
                     "
BASE COUNT         6746 a         6606 c         7301 g         6523 t
ORIGIN      
        1 ttttgctgtt gatggaagca tgattgtgta aagatgatat gtgtttgtgt aatcagtaga
       61 ttttataaga aaaccgccaa ggcggtgctt ggcggaggaa acggaggggc gggcggcatc
      121 agagcagctt ggaaacgacc tgtgcaaggg ctttttgcca gtctgacggt ttgatgccga
      181 agtcgttttc gattttgcgg cagtccaaaa tgctgtatgc gggcctgggg gcggcagccg
      241 cagagccttc atccgaaacg gctttcagtt cgggaacggg aaaagaggca tcctgttgca
      301 atgccgtctg aaagatgtgc cgggcaaatt cgtaccagga tacggatttg ccgccggcgt
      361 agtggtaaat gccgcgaacg ggattggact gctgcaacag gcggatgatg gcggcggaca
      421 agtcgccggc ataggtcggg cagccgattt ggttgtggac ggcggacagc ggggaacgtt
      481 cccgcgcaag gttcagcatc gtgcggacaa agttgtcccc gtattcgcta aacagccaag
      541 aagtccgcag gataaggctg tcgggattgg cagacagtgc gagcagttcg ccggcggttt
      601 tggattgtcc gtatacattg gaagggttgg taaagtcgct ttcctgatgg ggtattttcc
      661 cttttccgtc aaagacatag tcggttgaga tgtggatgaa tcgggcatgg gcgcgatgtg
      721 ctgccaaggc aaggttgtaa acggcggaag cattgacggc aaatgccgct gccgcatcgc
      781 cttccgcttt gtcgacggca gtataggcag ccgtgttgac aatagcgtcg ggttggaaac
      841 ttttgaccat gttgcagacg gcatcggcat cggtaatgtc tagggatgcg gaatccgtcg
      901 caatggtttc ccagtcttca ggaagacggt cgcgcaggca gtgtgccagt tggcttttcg
      961 agcctgtcaa taggattctc atgaggtatt tcctttggtg aaagtgtatt gtaggacttg
     1021 ctgtcggtat tatagtgcca aaactttgcc gacggttgac gggttggctt tttgtgccat
     1081 gggtattgtt ttgcgccgac ttcggctaga atatcggttt gcgattcaaa cctgtcgggt
     1141 cggtattttc aagatatttt caggggggtg cgaaatgagt gagattggtt tggtaaagat
     1201 ttattccgga aaagtgcgcg atttatatga aatcgacgat aaacgtatgc tgatggtcgc
     1261 ttccgaccgc ctgtccgcgt ttgatgtgat tttggacgcc ccgattccgg gtaagggcga
     1321 aatcctgacg cagatttcca atttttggtt taaaaaactg gcgcatatta tgcccaacca
     1381 tttcaccggc gataccgtgt acgatgtttt gcctgaaaac gaagccaaag ctttggaaaa
     1441 acgcgccgtt gtagctaaaa aactcactcc ggtgaaagtg gaggccattg tgcgcggtta
     1501 tctggcaggc agcggttgga aagattatca aaaaaccggc tcggtttgcg gtattcggct
     1561 gcccgaaggt atgcgggaag cgcaacaact acccgaagtg atttttacgc cctcaactaa
     1621 agccgcagtc ggcgatcacg atgaaaacat cagctttgaa gaatgcggac gcattatcgg
     1681 taaagaattg gcggaagaag tgcgcgacaa agcggttcgg ctttacaccg aagcggcgga
     1741 atatgccaaa tcgcgcggca tcatcatttg cgataccaaa tttgaattcg gtttggatga
     1801 agaaggtacg ctgacgctga tggatgaggt attgactccc gattcgagcc gcttctggcc
     1861 ggcggatcaa tatgaagtcg gcatcaatcc gccgtctttt gacaaacaat tcgtccgcga
     1921 ctggctggaa caaagcggtt ggaacaaaaa agcccctgcg cctaaagtgc ctgccgatgt
     1981 aattcggaaa acggtcgaga aatatcagga agcattgact ttactgacac aagattgatt
     2041 ttgaagactg aaggccgtct gaaaaggata tgtttcagac ggccttttta ttgtatcaat
     2101 actggatttt aaggatggtt gcctttataa tccgcaattg ttttcagcgt ccgaaatgcc
     2161 gtctgaaagc ttgtttacaa cctgccgcac ggtctgaaac cctaactatg cacattcgga
     2221 ttttagtgtg cattattagt gttttagcag tgcggtattt tgaaaggaac aatgatgttc
     2281 aataaatacg ttaagacctt ccaatacggt aatcaaaccg ttactttgga aaccggcgaa
     2341 attgcccgcc aagctgccgc tgccgttaaa gtatcgatgg gcgacactgt tgtttttgtt
     2401 gccgttacca ccaacaaaga ggtcaaagaa ggtcaggact tcttcccgtt ggccgtcgat
     2461 tatttggaac gcacttatgc cgcaggcaaa atccccggcg gcttcttcaa acgcgaaggc
     2521 aaacaaagcg aaaaagaaat cctgaccagc cgtctgatcg accgcccgat tcgtccgctg
     2581 ttccccgaag gtttctacca cgacatccaa atcgtagcga tggttgtgtc cgtcgatccc
     2641 gaaatcgatt ccgacattcc tgcaatgttg ggcgcgtccg ccgcgctggt gttgagcggc
     2701 gtaccgttcg ccggcccgat tggcgcggcg cgcgtgggtt atataaacgg cgtgtacgtt
     2761 ttgaacccga ctaaagccga gttggcgaaa tcacaattgg acttggtggt cgccggtact
     2821 tccaaagccg tgttgatggt ggaatccgaa gccaaaatcc tgcccgaaga cgtgatgttg
     2881 ggcgcggtgg tttacggcca cgatcaaatg caggttgcca tcaatgcaat caatgaattt
     2941 gccgacgaag tgaatccgga agtttgggat tggaaagcac ctgaaaccaa tgaagaactg
     3001 gttgccaaag tacgcgggat tgccggcgaa accattaaag aagcgttcaa aatccgtcaa
     3061 aaacaagcgc gttccgccaa attggacgag gcttggaatg cggtaaaaga agccttgatt
     3121 accgaagaaa ccgacacgct ggcagccaac gaaatcaaag gtattttcaa acacttggaa
     3181 gccgatgtcg tccgcagcca aattttggac ggacaaccgc gcatcgacgg ccgcgacacc
     3241 cgcaccgtcc gtccgctgaa catccaaacc ggcgtattgc cgcgcacgca cggttccgca
     3301 ctgtttaccc gtggcgaaac ccaagccctt gccgtagcga ctttgggtac ttcgcgcgac
     3361 gaacaaatca tcgacgcgtt gtccggcgaa tataccgacc gctttatgct gcactacaac
     3421 tttccgccgt actctaccgg cgaagtgggc cgcatgggcg cgccgaaacg ccgtgaaatc
     3481 ggtcacggcc gtttagctaa acgtgcgttg ttggccgtat tgccgaaacc tgaagatttc
     3541 agctacacca tgcgcgtggt ctccgaaatt accgaatcca acggctcttc ctctatggct
     3601 tctgtctgcg gcggctgttt gagcctgctg tctgccggcg tgcctttgaa agcacacgtt
     3661 gccggtatcg cgatgggtct gattttggaa ggcaataagt ttgccgtcct gaccgacatt
     3721 ttgggcgacg aagaccactt gggcgatatg gactttaaag tggccggtac gaccgaaggc
     3781 gttaccgcgc tgcaaatgga catcaaaatc caaggcatta ccaaagagat tatgcaaatt
     3841 gctttggcac aggccaaaga agcgcgtttg cacatcttgg atcagatgaa agctgccgtt
     3901 gcgggtccgc aagagctgtc cgcacacgcg ccgcgtttgt tcaccatgaa aatcagccaa
     3961 gacaaaatcc gtgatgtgat tggtaaaggc ggcgaaacca ttcgttcgat taccgctgaa
     4021 accggtactg aaatcaatat cgctgaagac ggtaccatca ctatcgctgc gactactcaa
     4081 gaagcgggcg atgcagcgaa aaaacgcatc gaagaaatca ctgccgaagt ggaagtgggc
     4141 aaagtgtacg aaggtactgt ggttaaaatc ctcgacaaca acgtcggcgc gattgtcagc
     4201 gtgatgccgg gcaaagacgg tttggtacac atcagccaaa tcgcacacga gcgcgtacgc
     4261 aatgtcggcg attacctgca agtcggtcag gtggtgaacg tgaaagcatt ggaagtggat
     4321 gacagaggcc gtgtccgtct gtccatcaaa gccctgctgg atgcgcctgt ccgtgaagaa
     4381 aatgccgccg agtaacgctt agggtgaaaa taccgtctga acaggtttca gacggtattt
     4441 tttacgggta tcgggaatga atggggctta cagccacagg acggcaagct tccataatgc
     4501 ccataatgat acggataatc ctgtacacag gcggatatat cggttttgca tgattttttt
     4561 cagttgcagg gaaaaaatgc cgattgccaa aagattgggc agcgtaccca gtgcaaaggc
     4621 aagcatatac agtccgccgg ttgtcgcact accgcttccc agcgcgtaaa gtgatgcgct
     4681 gtaaaccagt ccgcacggca gccagcccca taatattccg acagcaaggc aggcgggtat
     4741 ggattttatg ggcagcagcc ggttgagtat cgggttcagg ttgcgccata tcggtttgcc
     4801 gattttctcg atttttgccg ccaaggaaga aataccgctc aagtataagc ctaaaaagag
     4861 cagcaggagg ttggaggctg tgtataaaat attttgcagg acgcgggttt ggtcgagtga
     4921 aatgccgagt tgtccgatta atccgagcat cagaccgatt gccgtatagc tgcttatccg
     4981 tcctgtgtta agcagcagaa tcagccaaaa gcggttgata tgcgggggga gttggagcgc
     5041 aaacgcgctg cttaatccgc cgcacatacc gatgcagtga gttccgccga agaaaccgag
     5101 caggaacagg gtgaggaaag tgatgtcgtg gttcatggat ggctcatggt cagatgtttg
     5161 tcggggaaaa cggacaattt ccggcagtcc gaccaatagg gtttgacgag ggcattcccc
     5221 gtgctgttaa agtcttgaat aaggatgcgg tttgtgtcat gaatttcgac ggtcctgtaa
     5281 aatccgccct ttgctgtgct gtcggcgggt ttgccgtgtg cgtcaaaata caggacggtg
     5341 cggttgtgaa gatgcgcgca atttgaaacg gcggggcttg ccggtatgtt tcgggtgcag
     5401 gcggcaaggg ttgcccaagg gaaaagcagc aggaacaggc gggacattgt gtttcttgta
     5461 aggggtaaca aacagtataa tggctcattt taatcctcag gcggcgggag atggaagcat
     5521 ttcccttcgg gctcggggat ttcggattcg gaagcaacag acaatacggg atttcggaac
     5581 aatatgaaaa ctttgaaatt taccaaaatg cacggtttgg gcaacgattt tatggtgatt
     5641 gacgcggtca gtcaggattt tacccccgag gacgtgctga ttgcggcatg ggcggaccgc
     5701 ttccggggcg tgggcttcga ccagcttttg gtggtcgggt gttcggaaac cgaaggcgtg
     5761 gatttccgtt accgtatttt caatgccgac ggtagcgagg tcgggcaatg cggcaacgga
     5821 gcgcgttgtt ttgcccgttt tgttgcagac aagggtttga ccgataagaa agaaatttgt
     5881 gttgaaacgg ccaagggcat tatttttccg aaattgtccg ataacggtat ggttacggtc
     5941 aatatgggca aaccgaagtt tatgccgtct gaaataccgt ttgtccccga atcgggcgag
     6001 ggggatgatg cctgtattta cggggtgcat ctcgaatccg gcattcagcc tgtcagctgc
     6061 gtcaatatgg gcaatcctca tgctgtgatt gtagtcgatg acgtggaatg cgcgccggtg
     6121 cgcgaaaccg gttcgcgtat cgaaccgcac aggcagtttc ccgaacgcgt caacgtcggc
     6181 tttatgcagg ttgtcggacg gacggcaatc cgtttgcgcg tgttcgagcg cggcgtgggc
     6241 gaaacccaag cgtgcggtac gggcgcgtgt gcggctgtgg tggcgggtat ccgtctgggg
     6301 ctgttggatg aagggaaaac ggtagaggtg gttttgccgg gcgggacttt atatatcgaa
     6361 tgggcctgcg gcggcgatgt gatgatgacc ggccctgcgg aaaccgtgtt tgaaggcgag
     6421 ttggcgtatt catgattttg ctgcatttgg attttttgtc tgccttactg tatgcggcgg
     6481 tttttctgtt tctgatattc cgcgcaggaa tgttgcaatg gttttgggcg agtattgcgt
     6541 tgtggctcgg catctcggtt ttaggggtaa agctgatgcc ggggatgtgg ggaatgaccc
     6601 gcgccgcgcc tttgttcatc ccccattttt acctgacttt gggcagcata ttttttttca
     6661 tcgggtattg gaaccggaaa acagatggaa acggatggca ggcagacccc gaacatccgc
     6721 tgctcgggct ttttgccgtc agtaatgtat cgatgacgct tgcttttgtc ggaatatgtg
     6781 cgttggtgca ttattgcttt tcgggaacgg ttcaagtgtt tgtgtttgcg gcattgctca
     6841 aactttatgc gctgaagccg gtttattggt tcgtgttgca gtttgtattg atggcggttg
     6901 cctatgtcca ccgctgcggt atagaccggc agccgccgtc aacgttcggc ggttcgcagc
     6961 tgcgactcgg cgtgttggcg gcgatgttga tgcaggttgc ggtaacggcg atgctgcttg
     7021 ccgaaatcgg cagatgattg tgatgccgtc tgtatcggaa caaaaagtta taaagaaata
     7081 aattttgggt attggttttt taggcggcat aggtttagga taaagctata tccgaaattt
     7141 gtttatggtt cggcgcaaat cccctgcaat cggacaggat accgatgggg attgcgcctt
     7201 actgtcgaaa ccttattatt caggagcaga agatgaaaat tgcaaacagc atcaccgaat
     7261 tgatcggcaa cacgcctttg gtcaaactga accgtttgac caaaggtttg aaggcagagg
     7321 ttgccgtgaa actggaattt tttaatccgg gcagcagcgt caaagaccgc attgccgaag
     7381 caatgatcga ggccgccgaa aaagcgggaa aaatcaacaa aaacaccgtc attgtcgaag
     7441 caaccagcgg caatacgggt atcggtttgg caatggtatg tgccgcacgc ggctacaaac
     7501 tggcgattac catgccggaa agcatgagca aagagcgcaa aatgctgttg cgcacgtttg
     7561 gcgcggaact gattctaacc cccgccgccg aaggtatggc gggcgcgatt gccaaagcgc
     7621 aatccttggt ggacgctcat ccagacactt attttatgcc gcgccagttc gacaatgagg
     7681 caaatcccga agtccaccgc aaaacaaccg ccgaggaaat ttggaacgat accgacggta
     7741 aagtcgatgt cttcgttgcc ggcgtcggca cgggcggtac gattaccggc gtgggcgaag
     7801 tattgaaaaa atacaaaccc gaagttaaag tggttgccgt cgagcctgag gcttcccccg
     7861 tattgagcgg cggcgaaaaa ggtccgcacc cgattcaagg tatcggcgca ggttttattc
     7921 cgaccgtttt gaataccaaa atctacgaca gcattgccaa agtgccgaac gaagcggctt
     7981 ttgaaaccgc ccgtgcaatg gcggaaaaag aaggcatttt ggcgggcatt tcttccggtg
     8041 cggcggtttg gagcgcgttg cagcttgcca aacagcctga aaacgaaggc aagctgatag
     8101 tcgtgctgct gccttcttat ggcgaacgct atctctctac gccacttttt gcagatttgg
     8161 cataatgctt taatcggatt gtcgaaacat tcagacgcat ttttcggtat cggtgtaacg
     8221 ccgtgccgga aaatgcgttt ttgcatatat accgaaaacg ccggttgtgt tttaatcagg
     8281 tgttggcgcc gccgcattgc ttgagggaaa tattttttat tcgggtttta tccggcagga
     8341 cggattttgc cccaacggaa aatagccggc ctgcccgtaa agtcagccgt ttgtccgggc
     8401 gcagccgggg ctttgggctt cagacggcat attttcggaa tggcggcact tttgccggcg
     8461 gcgcggcagc catatgggga agggagggga tattgtggtc ggtaacggaa aaaaaatatg
     8521 ccgcaccatt gctggtgctg ggttgcgcgg tgttcggatt gggcagcctg attgtcagat
     8581 ccgtccccgt cggcccgtat gcaatcgcat tttggcggtt gctgatttcg gtattcgtat
     8641 tttggttttt ggcacggttt ttcggacaaa aattcccaaa aaacagaaaa accgtccgat
     8701 atgccctgac ggcgggcgtg tttctcgctt tcgatttggc actttggcac gaaagcatac
     8761 acgcggtcgg gccgggtatt tccaccctgc tcaacagcct gcaaatcttt ttcttgtcgg
     8821 caatcggtgt tttctttttc ggcgagcgtt tgggcaggct gcaggcggca agcttgatgt
     8881 cggcggttgt gggcgtggcg atgattgccg gtgcggaatt cggctacaac ggtaatgcgg
     8941 tttggggatt cgccagcggt ttggtgtccg ggctgatgct tgccctatct atggtgttcg
     9001 tccgcaagac tcatgaagtc gaacaggtgg cgcttttccc ttcaatgatg attttgagtt
     9061 tgggcggcgc ggcatcgccg attgttccgg cattgctgat ggacggcggc gcactttatc
     9121 cgaccacttg gaaagattcg ggtttggtgc ttgtgtacgg ggcgtggtga tgcagtgttt
     9181 tgcgtgggcg atggttgcct atgcgattcc gctgctttcc ctgtcgctga cggggctgtt
     9241 gcttttgtcc gaaccggtcg ccgccctgtt catcgattat ttcggattgg gcaaaacgat
     9301 tgaaggcgtg cagtgggcag gggtggtgct gacgctttcg gcgatttacc tcggttcgct
     9361 gaaacagccg tccgagcttt gactccggtc ggcatcagat tttctgccgt ggcaaacctg
     9421 cgtacgattg gactggcagg cagtcaaaac cggcaagacc aaacatgccg acaacggcgc
     9481 ggagcggaaa atgccgtctg aagccctttc agacggtatc ggggcgggca gaatcaaaaa
     9541 agccgcctcc gcaggaatgg caatgcaact aaaataatgt ccgtatttcc atatgccgcc
     9601 cacaccgtca gtcggacgaa atgcccttta ttcaatggga tgggtttcca cacaaacgga
     9661 aataaccggc ttttcacttg ccgacggata aaatcaaccg atgatttaaa cacaggcaaa
     9721 tcatacgccg cccgaacgca tcatttttcc aggcaatcca tatgtgccgc ccatatcgat
     9781 aatgccggca aggcggtgca tatagtggat taaattcaaa tcaggccaag gcgacgaagc
     9841 cgcggacagt acaaatagta cggcaaggcg aggccaggct gtactggttt agatttaatc
     9901 cactatatta tccccgaggc tgccggtagg gaaacaaggc gtttgatgat aaaaatgccg
     9961 tctgaaacag ggttcggacg gcatttttga tgttccggcg taattttccg gcttaacgga
    10021 ttgccgtgcc ggccctgccg aaatcgccga agttcatcaa aatgaacatt gccttgccga
    10081 caaccagctt gtcatccaca aagccccagt agcgcgaatc ggcactgttg tcgcggttgt
    10141 cgcccatagc gaaatagcgt ccttcgggaa ctttgcacac gaaaccgctg ccgtcgtcgg
    10201 catattggca atggttcaga ccgctttgat ctatggaata tccgttttca gacataatat
    10261 cggaggtata tttgcccaat acgggcaggg aaacggcagg ctgtccttct tttttcagaa
    10321 tattgaagga tttgccgtcc agaccgctgc ggaacatatc cgtgttgtgg atttcggatg
    10381 ggtcggtatc gtcaggataa cggtatgtgc cgtcaggaat gtcggaagcc ggttttccgt
    10441 ttaccgtcaa aactttatct cgatattcga caatgtcgcc cgggatgccg acaatacgct
    10501 tgatgtaggt catctccggc tgcaaaggat agttaaaaac aacgacatcg ccccgttcga
    10561 ttttgcccgt ggggatgaat acattgttta aaacgggtgc gcgcaggccg taggaaaatt
    10621 tgccgaccaa aatgaaatcg cccttgatta atcccgggcg catcgagctg gacgggattt
    10681 ggaacggttc ggcaataaac gaccggatga ggaacaatac caaaacggta gggaagaaac
    10741 tgccgaaata atcgccgaag tggctgcttt ccgagatttc gggatgagtc ttcaggcggt
    10801 atttatatac cccccaagcc gtaccgcaca atacaacgaa aatcaggaaa acggcggtaa
    10861 agctcataaa cagggacaaa gcggcaaaca caccgaccgc cgtcaggata taggcgtatt
    10921 cgaggccgga actccattca ccgttttcct gccgtttctt gtcgcttttg aaataaagga
    10981 tgatgccggc aagcagcgcg gcagccgcgc ccgacattag cattgtgttc attgttgttc
    11041 cttaacggtt aaaaacccgc ccggccgtgc aaccgtttta aggcggcaaa ttgcaaaatt
    11101 tgtttgcggg cgcgtgccgc tgaaatcagg gcggtttgag aggtgttccc gacgcgcccg
    11161 ccctatgtgc cgaaatatta tttgtcgctc acctgcaaaa tcgccaagaa cgcgctttgc
    11221 ggaatttcca cgttgcccac ttgtttcata cggcgtttgc ctgctttttg tttttcaagc
    11281 agttttttct tacgcgtaat atcgccgccg taacatttcg ccaaaacgtt tttacgcagg
    11341 gctttaacgt tttctcgggc gataatccgg ctgccgatgg cggcttggac ggcgatgtcg
    11401 aacatttggc gcggaatcag ttcgcgcatt ttcgatgcca gctcgcggcc tttgtgaacc
    11461 gcgctttgac ggtgtacaat caggcttaag gcatcgactt tttcgccgtt aaccataatg
    11521 tcgagcttaa tcaaatcaga cggttggaat tctttgaaat ggtagtccaa cgaagcataa
    11581 ccgcgcgaag tggatttgag tttgtcgaaa aagtccatta cgacttcgtt catcggcaag
    11641 tcgtaagtca gcatcacttg gcggcccata tactgcatat tgacctgcac gccgcgcttt
    11701 tggttacaca aagtcatgac gttgccgacg tattcctgcg gcacaaggat ggtcgcggta
    11761 ataatcggct cgagaatggt ttcgatgctg ccgatgtcgg gcagtttgga cgggttttca
    11821 acttcgattt tttcgccgct tttcaacacg acttcgtaaa ccaccgtcgg cgcggtggta
    11881 atcaaatcca tgtcgaactc gcgttccaag cgttcctgca cgatttctaa atgcagcagg
    11941 cctaagaagc cgcagcggaa gccgaagccc aatgcttgag aaacttcagg ctcgaatttc
    12001 aacgaagcat cgttcagctg caatttttcc aaagcgtcgc gcaaggcttc gtaatcgtgg
    12061 ctttccacag gatagagacc cgcaaatact tggctttgta cttcttggaa accgggcagc
    12121 ggctcagtgg cagggttggc aaccaaagta accgtatcgc cgactttcgc ctgtcccaat
    12181 tctttcacgc cggtaatcaa aaaacccact tcgccggctt ttagttcttg tttttgaacc
    12241 gatttcggtg tgaatacgcc cagctgctcg acctgcgttt ccgccttggt gctcataaag
    12301 cgcactttgt ctttcagttt gatggtgccg tttttcactc gaatcagcat gaccacgccg
    12361 acgtaattgt caaaccacga atcgacgata acggcttgca gcggcgcgtt ttcgtcgccg
    12421 gtcggcgcag gaattttggc aacgatttct tccaaaacgt cttccacgcc gatgccgctt
    12481 ttggctgaac actgcaccgc gccgacggca tcgatgccga tgatgtcttc gatttcctgt
    12541 tccacacgct cggggtcggc ggcgggcagg tcgattttgt tcaaaacggg cacgacttcc
    12601 acgcccaaat caatcgcggt atagcagttc gccacggttt gcgcttccac gccctgcgac
    12661 gcgtcaacga ccaaaagcgc gccttcgcaa gccgacagcg aacgggaaac ttcgtaagag
    12721 aagtcgacgt gtcccggcgt gtcaatcaaa ttaagctgat acacctgccc gtcgcgtgct
    12781 ttgtagttga gtgcggcggt ttgcgctttg atggtgatgc cgcgctcttt ttcgatgtcc
    12841 atggaatcga gcacctgcgt actcatttcg cgcaaatcca aaccgccgca atattggatg
    12901 aagcggtcgg caagcgtcga tttgccgtgg tcgatgtggg cgatgatgga gaaatttcgg
    12961 atatttttca ttgagttgtt tttaatgctg aacggtaggt tttagaaatg ccgtctgaac
    13021 aaacagcggt acgtccgaat atcgggcgca acgtggaaat agcccgatat tctaacggaa
    13081 aaccgctgtt ttggcatgat ttgataaagg ttttataaag atttgacgat ttctgccacc
    13141 atttttgcag aacttgccgc cgccgttttc aaaaactctt caaagctgat gtctgccttt
    13201 tcgtccgccg aatcggaaac agcgcggatg atgacgaacg gcgtttccaa ctgatgacag
    13261 gtttgggcaa tcgccgccgc ttccatttcc actgctttga cttcggggaa gtgcttgcgg
    13321 atttccgcca cgccttcgct gctgtggacg aagcggtcgc cgctgacaat caggccttgt
    13381 tctacctccg cgccttcaaa cgtccgcgcc gcccgttttg ccgtttcaat caatatgccg
    13441 tctgaagcga accttgccgg cagttgcggc acttgccccc gggcatagcc gaatgcggtt
    13501 acatcgacat cgtggtgctc ggtttccgtg ccgatcacca cgtcgccgac tttcaaaccc
    13561 ttgcccaaac cgcctgcgct gccggtgttg atgacgcagt ccggtgcgaa ttgacggata
    13621 agccaagccg ttgcaaccgc cgcgttgacc ttgccgatgc cgctcaatgc aagcactatg
    13681 cgttttcccg ccaattcgcc ttcataggcg gaaaatctgc cgaaagagac ggctttgaca
    13741 ttttccatca tctcgcgcaa aagctcgatt tcctgttcca tcgcgccgat aacggctact
    13801 gttttcaaag acatattgct aacctgctgt gaatttcgga tagaacgccg aattatacac
    13861 gctaacacgg caggattgag tggaggcggt ttgtccgtgc cgtctgaaac ggtttcagac
    13921 agcacggcgg atttttggta gaatgggaag gtacagattg tttgaagatt gggggacgag
    13981 gatgtttacc gatgaaaata tgactgcaaa ggaagaactg ttcgcatggc tgcgccatat
    14041 gaacaaaaac aaaggttccg acctgtttgt gacgacccat ttcccgcccg ctatgaagct
    14101 ggacggcaaa atcacccgca tcacggacga accgctgacg gcggaaaaat gtatggaaat
    14161 cgccttttcg attatgagtg cgaagcaggc ggaagaattt tcatcgacca acgagtgcaa
    14221 cttcgccatc agcctgccgg acaccagccg cttccgcgtc aatgcgatga tacagcgcgg
    14281 tgcgacggcg ttggtattcc gcgcgattac cagcaagatt cccaagtttg aaagcctgaa
    14341 cctgccgccg gccttgaagg atgttgcgct gaaaaaacgc gggctggtta tttttgtcgg
    14401 cggcaccggc tcgggcaaat cgacttcgct cgcctcgctt atcgactacc gcaatgaaaa
    14461 ttcgttcgga cacatcatca ccatcgaaga tccgatcgag tttgtccacg aacacaaaaa
    14521 ctgcatcatt acccagcgcg aggtcggcgt ggacacggaa aactggatgg cggcgttgaa
    14581 aaatacgctg cgtcaggcgc cggatgtgat ccttatcggc gaaatccgcg accgtgaaac
    14641 aatggactac gccatcgcct ttgccgaaac ggggcatttg tgtatggcga cgctgcacgc
    14701 caacagcacc aatcaggcgc tcgaccgcat catcaacttc ttccccgagg agcggcgcga
    14761 acaattgctg acggatttgt cgctcaacct tcaggcgttt atttcgcaac gcctcgttcc
    14821 gcgagacggc ggcaagggca gggtggcggc agtcgaggta ctactcaatt cgcccctgat
    14881 ttcggagttg attcacaacg gcaacatcca tgaaatcaaa gaagtgatga aaaaatccac
    14941 taccctgggt atgcagacct tcgaccaaca cctttaccaa ttgtatgaaa aaggcgagat
    15001 ttccttgcag gatgccttga aaaatgccga ttccgcacat gatttgcgtt tggcggtaca
    15061 gttgcgcagc cgcagggcac aaagttccga ccccgatttg gaactgctct gatggcggta
    15121 tggatttccg gacgggcagt ttgacatgat ttatccgtgg catcaggaac aatggcggca
    15181 gattgccgag cattggacga gccgtcctaa tgcgtggctg tttgttggca aaaaggggac
    15241 ggggaagact gcgtttgccc gctttgcggc gaaggcgctg ttgtgcgaaa cccctgcacc
    15301 gggctgcaaa ccctgtggcg aatgtatgtc ctgccatctg tttggacggg gaagccatcc
    15361 cgatttttac gaaatcacgc ctctggcgga cgaacccgaa aacggacgca aactgttgcg
    15421 gatcaaaatc gatgccgtca gggaaatcat cgataatgtg tacctgactt cggtacgggg
    15481 cggtttgcgc gtgatcctga ttcatcctgc ggaaagtatg aatgtccaag ccgccaacag
    15541 tttgttgaaa gtgttggaag agccgccgcc acaagtggtc tttttgctgg taagccacgc
    15601 ggcggacaag gttttaccga ccattaaaag ccgctgccgg aagaaggttt tgcccgctcc
    15661 ttcgcatggg gaagcattgg cgtatctgcg cgacaggggt gtggcggagc ctgaggaacg
    15721 tctggctttc cattcgggcg cgccgctgtt tcaggaggaa ggcgagcttc gggaattgag
    15781 ggcaaagctg ctggagatat tggcagagcc gagattgttg aagattttgg attacgccgc
    15841 gcttttcgat aaggaaaaac ttccgctcgc cgtatttgtc gggtggatgc agaaatggct
    15901 ggtcgattta ggcttgtgcc tgcaacacat gaaacccgtc tattatcccg cttatgaaga
    15961 caggctgctt cagacggcat cgggtttccg tccgcgcaat gtatttgcgg cggaggatat
    16021 gctcaaacag cttgcccctt acgggtttca tactttaaat gtcaaaatgc agatcgagca
    16081 tctgctcatc aactatttgg aattgaagaa agagaacagg tgaattatgt cagacggaca
    16141 aaatattccg gcaaaaatga tgtcgttgca gctgaaagac atgaatctgc tgtacagctc
    16201 ctatatgccg tttttggaac acggcggtct gtttgtgcag accgacgacg tattttccat
    16261 cggggacgat attctgcttg ccgtagaaat cctcaacttc cccaaactgt tcctgccgac
    16321 caaggtcgcc tggattaatc ctgcgagtac ttcctccaaa cccaaagggg tggggctggc
    16381 attcacaaaa cacgaaaact gcctgaaagt caaagaccag atcgaagtcg aactgggcag
    16441 cacaatcagc ggcagcagac ctacgtttac catgtaacgc tatgcatatc atcgattcgc
    16501 actgccacct caattttgaa ggtttgaaag aacgcctgcc cgaagttttg tccaacatgg
    16561 aagcaaacgg cgtggggcag gcactcgcca tcagcgtcag tagggaaagc ttctccgaag
    16621 tctttgttgt cgccgaagcg cacgaacaca tctattgcac cataggcgta catcccgaca
    16681 gcaaggaagc cgaagaattt tccattgcgg aaatggccga agccgccgcc catccgaaag
    16741 tggtcggcat cggcgagacg ggtttggatt attactggtg caaaggcgat ttgtcttggc
    16801 agcacaaacg ctttgcagac cacatcgaag cagccaatca aaccggactg cccgttatcg
    16861 tccatacgcg cgatgcggcg gcggacacct tggcgatttt aaaagaatgt aaaaccaatt
    16921 cgggtgtgat tcactgtttt tccgaggatg tcggttttgc ccgggctgcc atggatttgg
    16981 ggctttatat ttctttctcg ggaatcgtta cctttaaaaa cgcccccttg gttcaggagg
    17041 cggcgaaata cgttccggac gaccgcattt tagtggaaac cgatgcgccg ttccttgccc
    17101 ccgttcccaa acgaggcagg cagaacgaac cggcttttgt gcgtcatact gccgaacata
    17161 ttgccaaatt gcggaatcaa acattggaac aggttgcggc atatacgacg gaaaactttt
    17221 accggctgtt taaaaaagta cccgatatgc gggtcgtctg accctgtgcc gacgataagg
    17281 aaaaccatga aggcaattca tccgtatgca tgtccgcgct gctgccggct gcctgccaac
    17341 acgtttcgga caggcatggc aaattccgct tccaattctg cattgccaaa ggcggcagac
    17401 gggaagtaaa aaaagacgaa agcggcggcg gatatgccct gtgccatttg ccggacagca
    17461 ggattgtcga ggagtgggaa tattgccgtt cgcaacattg atactgcgcg atatacggca
    17521 aatattgtgg gaagtttccg cttttgcgta taatgcgccc tacctgacaa attttgtcaa
    17581 ctttatcaaa aggaataagc gatggcttcc atccacgacc aaattaaaga agtagtaacg
    17641 acacaccgcg tcgtattgtt tatgaaaggt acgaagcagt ttccgcaatg cggtttctct
    17701 tcccgcgccg tgcaaatcct gaacgcggca ggctgcaccg attacgttgc tgtcaacgta
    17761 ttggaaaatc ccgaagtacg ccaaggcatt aaggaataca gcgactggcc gaccatcccc
    17821 caactttatg tgaacggcga gtttgtcggc ggttcggaca tcctgatgga aatgtatgag
    17881 gcaggcgagc tgcaagagct gctgaaagcc tgatggattc ggcaatgccg tctgaacgtg
    17941 tttcagacgg cattttcttt tccggcaaat taaaaaaaag tataatgacg cgtctcaaaa
    18001 tcacactgga acaccgcgat gaacgttaac gttatcaacc atccgctcgt ccgccacaaa
    18061 ctgaccctga tgagggaggc ggattgcagc acctacaaat tccgtacgct gaccaccgag
    18121 ttggcgcgcc tgatggcata cgaggcaagc cgtgattttg aaattgaaaa ataccttatc
    18181 gacggatggt gcggacagat tgaaggcgac cgcatcaaag gcaaaaccct gaccgtcgtc
    18241 cccatcctgc gcgccggttt gggtatgctc gacggcgtgc tcgacctgat tccgactgcc
    18301 aaaatcagcg tggtcggttt gcagcgcgac gaggaaacgc tgaagcctat ttcctatttt
    18361 gagaaatttg tggacagtat ggacgagcgt ccggctttga ttatcgatcc tatgctggcg
    18421 acaggcggtt cgatggtggc caccatcgac ctgttgaaag aaaaaggttg ccgcaacatc
    18481 aaagccttag tcttggttgc cgcccctgag ggcgtgaagg ctgttaatga tgcgcatccc
    18541 gatgttacga tttataccgc cgcgctggat agccgtttga acgaaaacgg ctatattatt
    18601 cccggtctgg gcgatgcggg cgacaagatt ttcggcacgc gctaactgac tgattttcgg
    18661 atttgatatg aatttccaag actatctcgc cacatttcct tcaatcgacc atctgggcgg
    18721 tttggatgtt caggatgccg aaggcaaaac ggttcaccac attcccgccg ttcagggcaa
    18781 gctcggttcg ctcaagctgt acaatgcctt ggcggaacgt tttgatggaa aattgggtaa
    18841 agaagcggca gaacagggtt tgatatggtt tgccgaacat gttgccgacg cgcgcgccca
    18901 tccggggaag catccgaaca tcgatttgct ggaaaatgtc gtgcaaagcg gcgaaaccct
    18961 cctgctcaaa ccgcttgccg cgcaataatt ttcgaccatg ccgtctgaaa ttcgtttcag
    19021 acggcatttt gtcggaaaga agaccgtaaa acgggcattt tcttttctat ttggctgtgt
    19081 tttaacaatc cgttgatttt tgcactaatt tttgataaaa ttccttataa caaattgaac
    19141 tataagggat tttataatgt cgggcgtaac aaaagaatta gacattttaa aacaactttt
    19201 tgaaaatttg tccgatacgg ataaacaggc ttttttgact tctgtcagct caaaagaaca
    19261 ggttaaaaaa gtcattgaac caagaaaagt tacgaagtgc ccacattgcc aatctacaca
    19321 tttcgtcaaa aatggcaaag attgtggcaa ccaacgtttt ttgtgtcgtg attgtaaaaa
    19381 gtcttttgtt gagcagacag gcactattct ctacaacacg caaaaagaca ttgaagtttg
    19441 ggaaaaatac attcattgta tgattgaaaa atacccactt cgcaaatgtg ctgaaatttg
    19501 caaaatcaat cttgctactg cgtttacttg gcggcacaaa attttggacg cacttcaaaa
    19561 catgatgaat gaagttgagt tagacggcat tgtgcaagct gatgaaactt attcgacgat
    19621 ttcttacaaa gggcaccaca aaaattttaa tctgccacgt cctgctcaca aacgaggaac
    19681 gagagcaacg aaacgtggca tttccaaaga acaagtttgt gttccttgtg gtatcaattt
    19741 agacggtaaa tctgttgcta gaattagcaa tttgggtaag ccatctctaa aaaatatcaa
    19801 ctgattggca ttgaaaacaa cccaaaaatg tttgctcttg ccgccagtaa tatgatttta
    19861 cgcggagacg gtaaagccaa tttacaccaa agcagttgtt tcatgactga ttttcaggat
    19921 ttaatcaaaa atcccaaacc tgaaacaggc ttaaaacgcc caaatgttgg ctttctcaat
    19981 ccaccttacg cccaatccaa aagcgatgcc gaattgcatg aactttattt tgtcaaagaa
    20041 atgttggata tgttggcaga aggtggcaca ggcattgcca ttattccagt cagttgcgtg
    20101 attgcaccaa gcaaagccaa aagcgaaatt gtgaaatatc atcgcttaaa agccgtgatg
    20161 tctatgccga gcgaactgtt ttacccagtt ggcacggtaa cgtgcattgt cgtatttgaa
    20221 gcccataaac cgcattttca gacagtcgtg attgacccgg acacacaaga agaaatcagc
    20281 acgaaaaaag cctgtcgcaa aacgtggttt ggctactggc gtgatgacgg ttttgaaaaa
    20341 accaaacact tgggacgcat tgatttatac gaccgctggc agggcattaa agcgcgctgg
    20401 ttggaacatt atttaaacaa cgaagttcac acaggagaat cggtaacagc atttgtaact
    20461 gataacgatg aatgggttgc cgaagcctat ttggaaactg attattccaa aattacccga
    20521 gcagattttg agcaagtcgt gcgtgaattt gctttatttc aactactggg agcggaagta
    20581 gggccgactg aaaatttgga taatgaaagc tatgaagacg atgacaataa cgacttcgga
    20641 gacgatgaat aatggttgaa ttgcaagaga tttttgatgt gagttacggt tcaaaattag
    20701 atttgaataa aatgagcagc ttcaatccaa caatcaactt tgtaggcagg tcaggcaaaa
    20761 ataatggtgt aacagcatct gtggatttat tgaaaaatac aaaaccttat ccagcaggat
    20821 tattaactgt tgcattaggt ggttctgttt tatctacttt tttacaaaac aaaccatttt
    20881 atacagctca aaatgttgct gttttaaatc ccaaaacaga aatgacagaa caacaaaaac
    20941 tcttttattg tgctgctatt tttgccaatg cctaccgttt ttctgcttgc ggtagggaag
    21001 ctaaccgcac gttacgacaa ctgtttgtgc catctttgga tgagatccca agctgggttg
    21061 aaagcgtgaa tttaaatcca tctgcgggtg taaccgaacc gaaattaaaa gaatcgcttg
    21121 atttgcctgt ggttcgacaa tcaaaacgac ttgatgaaat ttttactatt caaaacggca
    21181 ttgccgcaac caaattaaaa gaatttgaac aacggcaaaa agacacggtt gtctatatcc
    21241 gtcctgccag tacgcaagca agaacgctac gaagttatat cgctcgtgat agcgttgatg
    21301 aaaaacatat ttttccatgc catacgcttt tcacttctac caatggcgaa ggtagccata
    21361 cttatagcta tgtttcaact tgtgaatttg tagcaaatag tgatgtcgcc gtattaacac
    21421 ccatacagtc cgatatgcca atagaagtaa aactgtatta cgctaaatgt attacagcaa
    21481 accgctatct gttttcttat ggacgtaaac ccaaaggcga aaaattaaaa agcattatgt
    21541 taccgtattt tgaccaacaa gaagattttg attatatctg ccgtttcatt cacaccttgc
    21601 tatttagtaa caatttgtag attaccaccc ttataaacaa aacaacccgc cgtaatagat
    21661 gaaatagaga actgtttttt agtgtgaatt catcttaagc aatcaacgga ttgttaaaac
    21721 acagccttct atttcaggat acgggcaatg atgtttcaac acacaggacg acacataaag
    21781 cgctgcccta tgtgttgccc taatttgaaa ggggttacac ccctttcaaa taaaatctga
    21841 tgctgctgcc acgaaggacg gatgtccgaa tggcggggtt tcaaccatta aggaaattat
    21901 gatgaaaaaa atgttccttt ctgccgcatt gcttctgtcg gctgccgccc aaaccgtgtg
    21961 ggcggatacg gtattttcct gtaaaacgga caacaacaaa tacatagaag ttcaaaaaat
    22021 caaccgcaat ctttacgaat attcgttcgg cagtgcggca aaaaaagaaa ttgccatacg
    22081 caacagcaaa gctgacctgt tggggcgttc cgacaggtgg caaggcatgg gcagcggacg
    22141 ccgggcaacg atgaaattcc aaaacggcga atttatgtac accgtatgga caggcttcga
    22201 ttccgtgact catacggaaa gcagcggtgt cgttgtggag cgtaggggca aggaagtcgc
    22261 acgggtaggc tgtacgccga aaaccgcgca ggcgaatttc aacgatgacg atttttcccg
    22321 gtaatcgggg cggataaggc gatggaaaca gcgaagcccg tcatgctgat tgtccgcccg
    22381 tcgggcaggg cgaaggacga tgtggaagtc tgccgtcgtg ccggatggca ggcggaagtg
    22441 ttgagtccga ttgaaatcga aaccgacgaa gcaagcctga aacggctgcc ggaaatgtat
    22501 gcccgtgcgg atgccgtgtt ttgggtcagt ccggccgccg ttgaaaccgc cgtcccgtac
    22561 cttaaccttt cagacggcat aaaggcgcac attgccgtag ggcaaggcag ccgccgtgcc
    22621 ttggcgcgtt atgcgggcgc gggcgtgttt gcacctgaag acggcaacga cagcgaggcg
    22681 gttttgcgcc tgccggtttg gaacagtctg cccgaaggtg cgcgcgtatt gtctgtgcgc
    22741 ggacacggcg ggcgggattt tctgatgaat gccttgcagg agaaaggttt tcggacggag
    22801 gtggcagaag tctatttcag acggcataaa cctttgaact ttcaaaattt ccaaaccgaa
    22861 aatattgccg ccgcctatat tacgtccaca gaacttgtgc agtcgctgtt tgcacagctt
    22921 ccgccgcaat tttcccgatt cttcaaatcc ttgctatact ttacccatca tccgcgcatt
    22981 gcggaggcat tgaagcgcga aggcgtgtgt tcggtcgaaa ccgtccctac gctggaagcc
    23041 gcgctttcct attcttccat ttccgtttca gacggcatgg tctttcccgg aacctcaaac
    23101 caataaggag caaaacggtg ggcgaacctg aaaacaaatc atccgaaccc gtacgcgaga
    23161 tacaggcatc aaaagaaatg ccgtctgaaa cctcttcccc acgcaaagaa aacgaaacag
    23221 aagtacacat tcccgccgct ccttttatcg tcaaacagtc cggcagcaac gctttggcgg
    23281 cctgcgcctt ggtattggcg gcattgggtt tgggtgcaag tggttttttg tttgtccaag
    23341 gacagaatgt cttgaaaaac caagagctgg cattcaacca gaaaatcgac aaagccgcct
    23401 tgggcgagtc ggaaaacgcc gccctgctca aagacaacct caaccgccaa agtgccatac
    23461 agtccgaact cgaccgtttg gacagcggtg tcaaagccaa cggcgaacaa atcctgatga
    23521 cgcaaaaagc ctatcgcgag ctgaccaaag ggcgtgccga ttggctggtg gacgaaaccg
    23581 agaccatact caatctggcg gcgcaacagc tggtgttgac cggcaatatc caaacggcag
    23641 tcggcgtgtt ggagcatatc gacagccgcc tgtcccgttt cgatcaggca gagcttctgc
    23701 cgatcaaaca ggcggtcagc agcgatttgg cggaactgaa aaaccgtccc tatgtcgata
    23761 tttccggcac ggcattgcgc ctcgaccggt tggaaaccgc cgtatccgga ctgccgctga
    23821 ttttggacgg cgtattgaaa ccgggcgtac aggcccgcaa cgaagccgtt tccgcttcat
    23881 ggtggcagaa cgtatgggaa aaatccctcg gcacattgaa ggggctggtc gaaatccgcc
    23941 gtttggaaaa caacgatgcc atgctgattt ctcccgaaca ggcatatttt gtgcgtgaaa
    24001 acctgcgtct gcgcctgttg gatgcgcgca ccgcattgat gcagcgcaac ggcgaagtct
    24061 atcagggcga tttgaacaat gccgaagccg ccgtcagaca gtatttcgat gccaagtcgc
    24121 ccgccacgca gtcgtggctg aaagaactgg cggaattgaa aacactggat gtacgcatga
    24181 cggcggatga cggcttgaag accagtttaa atgccgtccg cgcctatcgc gacggtacgc
    24241 gcatgacggc ggcggaaaat caagaagcgg aacaggcggc ttccgaaccg gcaaacgaaa
    24301 acacagcttc cgaaccggct gccgcatcgg atgtgaaggc catagaagca ccgtccctgc
    24361 cttcggaacg caaaccggaa cagcctgcaa aaaaacagcc cgcaccggaa aaggcagggc
    24421 gttcgccgtc cgctaaagga gaacgcgcat gaaaacggta gtctggattg tcgtcctgtt
    24481 tgccgccgcc gtcggactgg cgctggcttc gggcatttac accggcgacg tgttcgtcgt
    24541 cgtcgggcag accatgctca gaatcaacct gcacgccttt gtgttaggtt cgctgattgc
    24601 cgtcgtggtg tggtatttcc tgtttaaatt catcatcggc gtactcaata tccccgaaaa
    24661 tatgcggcgt tccggttctg cgcgtaaagg ccgcaaggcc gcgcttgcct tgaataaggc
    24721 aggtttggcg tatttcgaag ggcgttttga aaaggcggaa ctcgaagcct ctcgagtgtt
    24781 gggcaacaaa gaggccggag acaaccggac tttggcattg atgctgggcg cgcacgcggc
    24841 aggacagatg gaaaacatcg agctgcgcga ccgttatctt gcggaaatcg ccaaactgcc
    24901 ggaaaaacag cagctttccc gctacctgct gctggcggaa tcggcactga accggcgcga
    24961 ttacgaagcg gcggaagcca atcttcatgc ggcggcgaag atgaatgcca accttacgcg
    25021 cctcgtgcgt ctgcaacttc gttacgcctt cgatcggggc gatgcgttgc aggttctggc
    25081 aaaaaccgaa aaactttcca aggcgggcgc gttgggcaaa tcggaaatgg aacggtatca
    25141 aaattgggca taccgccgcc agatggcgga tgctgccgat gccgccgctt tgaaaacctg
    25201 cctgaagcgg attcccgaca gcctcaaaaa cggggaattg agcgtatcgg ttgcggaaaa
    25261 gtacgaacgt ttgggactgt atgccgatgc ggtcaaatgg gtcaaacagc attatccgca
    25321 caaccgccgc cccgagcttt tggaagcctt tgtcgaaagc gtgcgctttt tgggcgagcg
    25381 cgaacagcag aaagccatcg attttgccga ttcttggctg aaagaacagc ccgataacgc
    25441 gcttctgctg atgtatctcg gccggctcgc ctacggccgc aaactttggg gtaaggcaaa
    25501 aggctacctt gaagcgagta ttgcactgaa gccgagtatt ccggcgcgtt tggtgttggc
    25561 aaaggttttt gacgaaaccg cacagtcgca aaaagccgaa gcacagcgca acttggtttt
    25621 ggcaagcgtt gccggggaaa accgcccttc cgccgaaacc cgttgaaacc ttcagacggc
    25681 atcgttgaaa tgccgtctga aaaccatttc acttgacaaa cagattgaca gaaaacccgt
    25741 atgactcttt taaaaaacga taccttcctc cgcgccctgc tcaaacagcc cgtcgaatac
    25801 acaccgattt ggatgatgcg ccaggcgggg cgttatctgc ccgaatacaa agccacgcgc
    25861 gcgaaagcgg gcagcttcct cgatttgtgc aaaaacaccg gattggcgac cgaagtcacc
    25921 atccagcctt tggaacgctt cgatttggac gcggcgattt tgttttccga catcttgacc
    25981 gttcccgatg caatgggctt ggggctgtat tttgccgaag gcgaaggccc gaaattcaaa
    26041 cgcgccttgc aacacgaatc cgacatcgcc aagctgcacg ttcccgacat ggaaaaactg
    26101 caatacgttt tcgacgcggt aacttccatc cgtaaagcat tggacggccg cgtaccgctt
    26161 atcggcttct ccggcagccc gttcacgctc gcctgttata tggtcgaagg cggcggcagc
    26221 aaagaattcc gcaccatcaa aaccatgatg tactcgcgcc ccgatttgct gtacaaaatc
    26281 ctcgatacca acgcccaagc cgttaccgcc tacctcaacg cccaaatcga cgcgggcgcg
    26341 caggcggtgc agattttcga cacttggggc ggcgtgttga gcgacgcggc gtttaaagag
    26401 ttcagcctca aatacatccg ccaaatcgtt gccggactca aacgtgaaag cgaaggtcgc
    26461 cgcgtgcctg ttatcgtgtt tgccaaaggc ggcgggctgt ggctggaaag catggcccaa
    26521 atcggcgcag acgcattggg cttggactgg acgtgcaaca tcggcgaagc acgccgccgc
    26581 gtcggcaacc aagtcgccct gcaaggcaac ttcgacccgt ccgccctctt cggtacgccc
    26641 gaatccatcc gcaccgaggt cgcacgtatc ctgaccggct acggacacgg cagcggccat
    26701 gtcttcaacc tcggacacgg catcaaccaa cacgccgacc ccgaacacgc caaaatctta
    26761 gtcgacaccg tacacgagct gtcgcggcag tatcacggcg ggtaagccgg caggaaaccg
    26821 cccgatatgc cgtctgaaga atcggaagtt ttcagacggc atatgcttca ttttgcactt
    26881 tggtacacca agccattgtg tggtagcttg atattgggta accaaaaatc ttttccctgc
    26941 caacctttgt ctttatttcc tactacttga taaagcgtaa ggaataaatc attgggatat
    27001 ttttctccta atttacggtc gtcttccgaa agcattgtgc cggttgctcg agaaagttta
    27061 cgtccggttt tgatcaaaac ataggtttta aaacttggac gttgtgcttt taatgcttct
    27121 acaccagcaa taaatttttc cttattccag tcatctggaa cataacgacc caattt
//
LOCUS       DAXFWM010000021        26208 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000021 DAXFWM010000000
VERSION     DAXFWM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26208
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.021"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            113..2140
                     /locus_tag="ACFI8B_000883"
     CDS             113..2140
                     /locus_tag="ACFI8B_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC3834145.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2229..3782)
                     /locus_tag="ACFI8B_000884"
     CDS             complement(2229..3782)
                     /locus_tag="ACFI8B_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC3834146.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            3833..3961
                     /locus_tag="ACFI8B_000885"
     CDS             3833..3961
                     /locus_tag="ACFI8B_000885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834147.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAACRRFAPVLPMSDLNAV"
     gene            complement(4033..5226)
                     /locus_tag="ACFI8B_000886"
     CDS             complement(4033..5226)
                     /locus_tag="ACFI8B_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC3834148.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLNSRSAAADFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5223..6206)
                     /gene="patB"
                     /locus_tag="ACFI8B_000887"
     CDS             complement(5223..6206)
                     /gene="patB"
                     /locus_tag="ACFI8B_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC3834149.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6217..7653)
                     /locus_tag="ACFI8B_000888"
     CDS             complement(6217..7653)
                     /locus_tag="ACFI8B_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC3834150.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGTVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7783..8948)
                     /locus_tag="ACFI8B_000889"
                     /pseudo
     CDS             complement(7783..8948)
                     /locus_tag="ACFI8B_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9389..9601
                     /locus_tag="ACFI8B_000890"
     CDS             9389..9601
                     /locus_tag="ACFI8B_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC3834151.1"
                     /translation="METLLLDIVGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9580..9741)
                     /locus_tag="ACFI8B_000891"
     CDS             complement(9580..9741)
                     /locus_tag="ACFI8B_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834152.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9749..11327
                     /locus_tag="ACFI8B_000892"
                     /pseudo
     CDS             9749..11327
                     /locus_tag="ACFI8B_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11409..12506
                     /locus_tag="ACFI8B_000893"
     CDS             11409..12506
                     /locus_tag="ACFI8B_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC3834153.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12620..13735
                     /locus_tag="ACFI8B_000894"
     CDS             12620..13735
                     /locus_tag="ACFI8B_000894"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC3834154.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTAGNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNPASVSIENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVPPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGKGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSFAEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            13803..14315
                     /locus_tag="ACFI8B_000895"
     CDS             13803..14315
                     /locus_tag="ACFI8B_000895"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC3834155.1"
                     /translation="MPSERVSASDGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            14392..14868
                     /locus_tag="ACFI8B_000896"
     CDS             14392..14868
                     /locus_tag="ACFI8B_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC3834156.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14856..15782
                     /locus_tag="ACFI8B_000897"
     CDS             14856..15782
                     /locus_tag="ACFI8B_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3834157.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDGLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15847..16404
                     /locus_tag="ACFI8B_000898"
     CDS             15847..16404
                     /locus_tag="ACFI8B_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3834158.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            16491..18548
                     /locus_tag="ACFI8B_000899"
     CDS             16491..18548
                     /locus_tag="ACFI8B_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC3834159.1"
                     /translation="MDKFKPRILKCCQPNPTQPNPSPDLSDTAGANTEAIYTSDGITA
                     NSTQLEQLKKLFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNL
                     PPETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYNTGS
                     DGFVYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYIAREL
                     LKDDGVIFISIDDNEAAQLKLLCDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWI
                     FVFAKSCQFIAATKGKERRYYETDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTG
                     KKYPANPNATWRVTKDTFQDYYNKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDF
                     GKVAVSSRLPENVGTLADAVAEYLAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPK
                     PSQLIKFLVSISSKSNDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAE
                     KSEARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFS
                     PEQPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLLNK
                     GFTSADLLAFIKKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYTNKKEIELNVI
                     IRV"
     gene            18638..21577
                     /locus_tag="ACFI8B_000900"
     CDS             18638..21577
                     /locus_tag="ACFI8B_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC3834160.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(21671..22630)
                     /gene="ttcA"
                     /locus_tag="ACFI8B_000901"
     CDS             complement(21671..22630)
                     /gene="ttcA"
                     /locus_tag="ACFI8B_000901"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC3834161.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGP"
     gene            complement(22722..23585)
                     /locus_tag="ACFI8B_000902"
     CDS             complement(22722..23585)
                     /locus_tag="ACFI8B_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC3834162.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQAGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAE"
     gene            complement(23619..23981)
                     /locus_tag="ACFI8B_000903"
     CDS             complement(23619..23981)
                     /locus_tag="ACFI8B_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC3834163.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            24056..24535
                     /gene="ybaK"
                     /locus_tag="ACFI8B_000904"
     CDS             24056..24535
                     /gene="ybaK"
                     /locus_tag="ACFI8B_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC3834164.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            24557..25249
                     /locus_tag="ACFI8B_000905"
     CDS             24557..25249
                     /locus_tag="ACFI8B_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC3834165.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIIQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLIIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            25253..26128
                     /locus_tag="ACFI8B_000906"
     CDS             25253..26128
                     /locus_tag="ACFI8B_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC3834166.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
BASE COUNT         6409 a         6510 c         7226 g         6063 t
ORIGIN      
        1 tttcggccgg tttgtgcatt tcgtcatccg gaagccggta ttttgccggc aggttttgac
       61 tactttgccc gatgaccggg tttgtctgtt tttaacaaag gagtgagaag gtttgtccct
      121 gtctgaattt atagaacgcc gaacgtcatt taatccgatg gttattttga cgactttgtt
      181 ttttgtgtgt gttttggtgg tattggtttt aaccgtgccg gatcaggtgc agatgtggct
      241 cgaccgggca aaagaagtca tttttaccga gttcagctgg ttttatgttt taacgttttc
      301 catttttctg ggttttctgc tgatactctc ggtcagcggt ttgggaaaca tcaggctagg
      361 acgggatgaa gatgtgccgg aattcggctt cctgtcgtgg ctggcgatgc tgtttgcggc
      421 cgggatgggc gtgggcctga tgtttttcgg cgtggcagag ccgttgatgc attatttttc
      481 ggacattacg gtcggcgcgc cggaacacag gcagcagcag gcattgctgc acacggtgtt
      541 ccattggggc gttcacgcct ggtcggtgta cggtacgatt gcattggctt tggcttattt
      601 cggtttccgc tacaaactgc cgcttgccct gcgttcttgt ttttaccccc tgttgaaaga
      661 aaaaatttcc ggaaggttcg gcgatgccat tgatattatg gcgttgcttg ctactttttt
      721 cggcatcatc accacattgg ggttcggggc ttcgcaactg ggcgccggat tgcaggaaat
      781 gggctggatt gccgaaaaca gcttcggcgt gcaggtcttg attatcgccg ccgtaatgtc
      841 cctcgccgtc gtttcggcaa tatccggcgt ggggaagggc gtgaaggtgt tgagcgagtt
      901 gaacctgggc cttgcgtttt tgctgctgtt ttttgttttg gcggcgggcc ccactgttta
      961 cctgttgtcg gcattcggcg acaacatagg gaactacctc ggaaatctgg tgcgcctcag
     1021 tttgaaaact tatgcgtacg aacgggaaca caagccgtgg tttgaatctt ggacggtgct
     1081 ttattgggcg tggtggtgtt cttgggcgcc gtttgtgggt ttgtttatcg cgcgcatttc
     1141 aaaggggcgc accatccgcg agtttgtctt cggggttttg ctcatccccg gcctgttcgg
     1201 cgttttgtgg tttaccgtct tcggcaatac ggcgatttgg ctgaatgacg gggttgcggg
     1261 gggaatgctc gaaaagatga cctcctctcc ggaaacgctg ctttttaaat tctttaatta
     1321 cctccccctg cccgaactga cgagcatcgt cagcctgctg gtcatttccc tgttttttgt
     1381 aacttctgcc gactccggga tttatgtcct gaacaatatt acctctcggg acaaaggctt
     1441 gagcgcgcca cggtggcagg cggttatgtg gggcgtgctg atgtctgccg ttgccgtttt
     1501 gctgatgcgc tcgggcggac tcggcaacct gcagtctatg accctgattg tttccctgcc
     1561 gtttgccctg ctgatgctga taatgtgttt cagcctgtgg aaaggcttga gtgcggataa
     1621 gaaatatttt gagacccggg tcaaccctac cagtgtattt tggacgggcg gcaagtggaa
     1681 agaacggctg gtgcggataa tgagccagac gcaggagcag gatattttaa aattcctcaa
     1741 acataccgca tcgcccgcta tgcacgagtt gcaacgggag ctttcggaag aatacggctt
     1801 gagcgtccgg gtcgataaga tgtttcatca ggacgagccc gcaatcgagt tcgtcattcg
     1861 gaaagagacg atgcgcgatt ttatgtacgg gattaagtct gtcgggcagg atgtatccga
     1921 ccagttgatt aacgacggca agctgccgca tatccggcac cagacaactt acaaacccta
     1981 cgcttatttt ttcgacgggc gcgtcgggta cgatgtgcag tatatgaaca aggacgagct
     2041 gattgccgac attttgaaaa actacgaacg ttatttgatg ttgttggatg atgtcggtca
     2101 ggaactgatg gcgcacgagc aggtggaatt ggcagagtaa gcgccttccc tccggcgttt
     2161 gccaaaaaat gcggtgaatc agattcaccg catttttttg ccggacgggg cttcggacgg
     2221 cacggcgttc atttgtttcc gtcaaactgc tccttcagca cccgtttcaa tactttgccc
     2281 gtagcgttgc gcggcagccc gtctttaaag tggatctgtt tggggatttt gaaatttgcc
     2341 agcacggtac gcaggtggcg gcggatttcg tcctcgccca aatccatacc ttccttcaat
     2401 tggacgaagg cgacgatttc ctcgtcggca taacggtctt tcacgccgat gacggcggcg
     2461 gcttcgacgg catcgagttt gtagatttct tcttcgatct cgcgcggata gacgttttga
     2521 cctttggaaa taatcaaatc ttttttgcgg tcgacgataa agataaagcc gtcctcgtct
     2581 atggtaacga aatcgcccgt tttcaaccag ccgttgacga tggtttcatc ggtggcggca
     2641 ggcatattga ggtagccccg catcaccgaa ccgcccctga cgatcagttc gcccacttcg
     2701 ccgcgcggca cttcgaccaa ttcttcatcg acggctttgg cttccaaacc gggcaggggg
     2761 atgccgacgc tgcgggcttt ttgccgttcg ggcgtattga cggcgacgac gggcgaggct
     2821 tcgctcagtc cgtagccttc cagcaatttg gcgcggggga acttggcttt aaaatcgagg
     2881 atggtttgtt ccgccaaagg cgcgccgccg ctgataaaca ggcggatgcg gttgaaccat
     2941 ctgaaatacc aagggatttt cgccttgctc atcgcggtgt aaatcgcggg tacgcccaaa
     3001 aacacggttg cgcgtttgag cagggcctgt ttcaaaacgt tggaaaaggg gaaaacggat
     3061 ttgaccaaaa taatcgaaca cgccatataa atcggcagca gtaccatagc cgtcagcgtg
     3121 aagctgtgga acatcggcag gaaaacgata aagcggtcgc gtttggaaat tttaaagatg
     3181 cgttcgatgc cgttcaggtt ggcgaacagg ttggcgtaac tgattagcgc gcctttggga
     3241 tgccccgtcg tgccggaggt gtagatgatg tgtgccaaat catttatccg gggttggcgg
     3301 tccaagtcgg gtttttcggg gaagcggcgc acgttttcaa aaaaggcatc gccttccgcc
     3361 gtttcgccgg ccggccggct tttgtccgtc caaatgattt tttcgacggg cgtttgcgcc
     3421 ttcaggcccg ccaattcttt tgacaggccg gccgaggcga acaggaagcg cgccttgcag
     3481 tcattcagga tatacgcgta ttcgctgttt ttcaaaaatg tgttcatcgg tacggcgacc
     3541 gcgccgatgg cggagacggc gaaataggcg gtaataaatt ctgtggaatt ggaaaccgcc
     3601 agcgcgaccg tgtcgccgaa cttcacgccg atattttgca gatacgccgc gacggcttcg
     3661 gcttcctgct tgagcgcgcg gtaggcggtt ttttccttgc cgtcgaacac tgccgtgccg
     3721 ttgccgtttt tgcggcaggc ggcggcgagc atttcgtaga aattggcata agtccggttc
     3781 atgaaacttc ctttatggtt gcggacggcg aacgccctca tttgaaaaca atatgccgtc
     3841 tgaaaacgga aggcggcagt ttatcatctt tgccttcaga cggcatatca aacgccgcct
     3901 ttgcgccgca tgccggaggt tcgcccccgt ccttcctatg tctgacctga atgccgtctg
     3961 aatcaatatg aagatatacg tttcagacgg catccccctg ttgtggaaat accgtccgcc
     4021 ccccctgtcc gattattgcc tgattgcggc ggcgcggacg agttcttcga ggctgtcggc
     4081 aagcatttcc gccgcgcgcc ggtagccttg ggcggagaag tgtacgccgt ctttggcggc
     4141 ccatccttgg ttgagccagt ttttcatgct gcatatgccg cccattgcgt tttgccaaga
     4201 ccaaaacatc gtctgcccct gacgggcgac gcgccgctgc atctgttgga cttcggtcag
     4261 gaggacgggg cgcgtgccgc atacgccgag cgtgtttttc agggattcgg gcgcgccgat
     4321 gatgaggatg ccggcggcgg gcaggctgtc gcggatttgg cggacggtat ccagccattt
     4381 ttgttcggta tcggcaatgt cgatgttgtt gttgaaggct tcgttggtgc cgtaggaaag
     4441 gataaccaaa tcggcgccgg tttgggcaag gtcgttcata cggtcggcac gccatttcga
     4501 ccactgggtc aattgtgcgc cgttgatgcc catcgcggaa acggtaatgc cgccggcggg
     4561 attttcgatg ttgatgaagc cgatgtccca cggcatttcg gtctgtatgg ccaggggcag
     4621 tgccgcgccc gtatccagta cctgccagcc gccgccgttg gcggagacgg tgttgccgtt
     4681 gacggtcagg gtttgttcgg cgagcagcgg tttggcaaac agggaaacgc gctgtttgcc
     4741 ggttttgccg tcagacgcgg tcagggtcat gccgccgccg ctgccggttt gggcgaggat
     4801 gccgccgagg gggaagtctg ctgcggcact tcggctgttg aggctttgcc agttgccgtt
     4861 atagcggacg gccgccatgc gctgcccttt gacattggcg gggtaaaccc agcctatgcc
     4921 gccgtcgccc catgtttttt gcaggcgttt gcgcagggcg tcggtaaaga agtcgccggc
     4981 ggtatgcgag tcgccgattt gcaggatgcg gaaggtctcg ccgctgcctt gtgcgacgga
     5041 tcggagtttt ttcatccaag gcgaggcggc ggcgttgccg tagtcggtca gcagcccgtt
     5101 tgtatcggtg tagggggcgg acggggaaac ggtaacggtg tcggggctga cggaggcgac
     5161 gggcagggct tctgcctgcg tggcggcgaa cagggcggaa aatgcgatga agtgtttggg
     5221 gttcatggct gtgtacttga tggttgcgta ctcggttcaa aaacgatttt ttccattatt
     5281 ttttccgcca gcagtttttg tccttcggcg gtaaagtgta tgccgtcctt actgcggtag
     5341 cggacgggtt tgccgttgac gttgacggaa tcggtgtagc ggcctttccc gccgctcagt
     5401 gtttgcgcgg tgggaatcag gatgattttg cctttcaagt gttccgaaag cagtttgtcg
     5461 aggtagcgca tctgaccgtc gagcttgact tttttcatgt aggggatgcc gagccagacg
     5521 acttggacgc ggtgcgtgtg tgcggcttca aggatgcggt cgacgcgttt caggtattct
     5581 tgcgcccatt cgtcggaagc gaatttgagg tagcgtttgc cgacggggaa atcccacggg
     5641 tcgttcgggc cgaggaagac ggcgagtacg ctgatttcgg gatgtttttt caaggtttct
     5701 tcaatcgttt tcggccagtc gaagaatgag ggataggaaa gccccgtgct ttgtttgctg
     5761 aggttggcgg attcgatgcc gtattgctgt ttcaggcttt tttgcacgaa aggcgcaacg
     5821 ccctgcatca gcgaatctcc ggcgaaaaag actttgtcgc cgctgcggac ggcggcggct
     5881 tcggtgcctt gtttccattc tgttccgccg gccggcgggg cggcttcgga tgcggcggct
     5941 tcaggcggca tatctgccga accgccgtct tgagccgtcg gcggcgtttc gccggacagg
     6001 aaggttttga tgccgtctga aagggcgtag gcgttttctt gcaacgccgc tccgctccgc
     6061 caccatccgt aggcggcaag cggttcgagc gggctgttgc ggtggtaggt ctgctgccag
     6121 taggcgttga tggggttttg gctgaaccac acggcaatca gggcagacat cagtatggag
     6181 gcgaaaaggg aaagaaagtt tttcatgtcc aaacccttaa aaattggcat aaataaagcc
     6241 gggtatcccc gaaggggcga ggacgataat cagcagcagg atgatggaaa tctggataaa
     6301 ccacagccac atcgggattt tttccaaacc tttgaccgtg ccgtcgaaag cgcgttgcag
     6361 gtaagggtag agcagcatca aggatgcaaa cgaggcaagc agcagcatat ccgcccgttg
     6421 cggcgcgttc cagccgccgg cattggcaaa gagggcactg aaaactgcgc ctgcatcgtc
     6481 ggggtttgcg gtattgaaga cgacaaagct aaggcagacg aaatggaagg taacaaacca
     6541 cgagagcggc gcaaggtatt tcagacggca tagcgcgtcg cgtccgaaat accggtcgcc
     6601 cgtgttgagc agcgccagtg ccgtgccgtg cagcgcgccc caaatgagga agttccagcc
     6661 gtagccgtgc cagatgcctg agagtaccat tgccgccatc aggttgagct gtgtccgcaa
     6721 aaagcctttt ttgctgccgc ccaaggggat gtagatgtag tcgcgtatcc aggtggaaag
     6781 gctgatgtgc catttgtccc aaaatgcgcg gatattcgcg gcacgaagcg gtgcggagaa
     6841 gtttttgggc agccggaagc ccagcagcat cgccataccg ataaccaaat cggaatatcc
     6901 ggaaaagtct aaaaagagtt ggaaggtata gccgtacacg cctgccaaca cgccccagcc
     6961 gtcgaattgg gcgggatttt caaatacggg cgacacccag ttttccgcca gcatccccgc
     7021 cagccaccat tttttggcaa tgcccagtaa aatcagggaa acggcgaggg cggggcggac
     7081 gggcgaacgc ggtcggcggg tgcggatttg cgccagtgca cccgcctgtt cgccgtccgt
     7141 gcttttgaat gcggcggcgc ggataatcgg gccggaggta acggtgggga aaaaactcag
     7201 gtgcagcagc agctcgtgcc acccaaaacg cgcggcgtgc ggggcgcgga agcagtaaac
     7261 cagataggcg acagactgga aggtataata ggaaagcccc agcggcatca ggatgtcgat
     7321 tgcgccgcct tttccggcat attgtgcaat cagcggacgg aaaaaatcga aatatttgaa
     7381 aaagcccaaa acggtaatcg aggcggcaat gccgcacccc agccaaaagc ggcgcgtact
     7441 ttcgcgatcg gaacggagca gttcgcccaa aaggtacacg caggaggaat aaaggacgat
     7501 gattgccgca aatacggggc taatatggta gagccagccc ataccggcag ccaaaagcag
     7561 caggttttgg acggacgggt atttcgcaaa gccccagtaa atcggcagga agacgataaa
     7621 aaacagtgcg aactcgacag acagcagcgg cataaaccat ccttgggaca aaaaaatagg
     7681 cgttaaaaca cggcaaagcc cgttatttta agcaggggcg gatattcctt caatcaaaat
     7741 gccgtctgaa gggcgggagg ggcttcagac ggcatcgctc ggtcaggttc ggatatggtg
     7801 gttcaaattc aggcagaccc gcaaacctgc cgccgccgtc agcatacccg caacgccgat
     7861 gcaggaaatg cccgagtatt gcgtaaccta atgtcccagc agtgcgccgc cgcctatacc
     7921 gacgttgtaa agctccgaat aaatcgagtt ggcaaggtct gcggcatcgg aggcgaaatc
     7981 caaaactttg gcaaccatcc ccaagctgac gaccacaatc gccgtccccc atacgaacac
     8041 caacgcgtaa atggcggcag gaaaatgcgc caaaggcagc aaaagccccg aagagagggc
     8101 gataagcgac accgcgcccg ccaaaaatgc gcgcggatgt ttggcaaacc atttgccgaa
     8161 caggtaagac gcggcaaaac ccgccaggcc gtacagcccc ggaaccaccg taacctgccg
     8221 cgcggagaaa ccgccgattt ggatgacgaa gggctcgatg tagctgtatg cggtaaagtg
     8281 cgccgtaatc accggcaccg ttaccgcata cagcagcatc agttttttcc gtttcaaaag
     8341 caggggcagg ctgcttaaag aaccggtatt cacgctcggc aggcgcggca ggcttttggc
     8401 aagcactgcc atcaccgccg ccgaacacag gccgatcagc agaaaactcg cctgccagcc
     8461 caaatattgt ccgaccatac gcccgagcgg aatccccgcc gccatcgcca tcaccgtccc
     8521 cgtgctgagc agccccaatg cctgattccc ttttccgaca agggcgatgc gtaccgccag
     8581 cgaagccgta atggaccaga acaccgcgtg cgtcagcgca atccccacgc ggcttgcgag
     8641 taggatttca aaacgccacg aggcaaacga caggatgtgg ctgacagtaa tacagcgcaa
     8701 acaggaccag cagcaggccg cgccgttcca taattgcgcg tcagcagcat cagcggcagc
     8761 ggcagcgaag tcagcgcgac aatccacgca taaaccgtaa tcatcacacc cgtttcggtc
     8821 gccgccatgc cgaagcttcg tccgatgtcg ctcaaaagcg cgatggggat gtattcgacc
     8881 gtgttgaaaa taaatgcgcc gattgccaag gcaacgatgc tcaaccaagg tttgtgcgaa
     8941 gcgttcatct caattcctta tatgtcgcac cccgccaaaa gggggcgcat tatggtgaat
     9001 taacaaaaac cggtacggcg ttgccccgcc ccggctcaaa gggaacggtt ccctaaggtg
     9061 atgaagcacc gggcgaaccg gttccgtacc atttgtactg cctgcggccc gccgccttgt
     9121 cctgattttt gttaattcac tatacttttc ttgtctgccg gacggcaaaa tccaaatcag
     9181 ggggaagcag ccgcctcccg ttcggagaaa cactcgggac gctgctttga aaatcgttcc
     9241 atatgttgaa acattgccgc ctcccgattg ggcaaggttg ccattatgtc gcaagcatcc
     9301 gtgccgcacc gtttccgcgt ttgcccaagt tcacgctccg acttacaata acgcccgtca
     9361 gattgcagta ccaacgaaag gaaaaatcgt ggaaaccctc ctcctcgaca tcgtcggcat
     9421 gagctgcggc ggctgcgtca aaagcgttac ccggatatta gaaagcgtaa aaggcgtggc
     9481 aagcgtcgaa gtcagccttg aaaacaaaag tgcgaccgtc ggatacgacc ccgcgcaaac
     9541 cgcagcggaa gcactaatcg aagccgtaga agacggcggc tatgatgcgg cgttgaaata
     9601 aagcggcaaa aatgccgtct gaagcctttg cgtttccccg atgccgtctg aagcacgctc
     9661 cgcgccttcg ggcggcattt gatttagttg aacgggcagg atgaattggt taaacttgcg
     9721 ggcaatagtg ccttcagaca aacaaaatat gccgaacaaa aagaaaaaag ccgcctttgc
     9781 gcgctgcaac ggagaacggt gtgccgggcg tgtccgacat caaaaattca tatgatgatt
     9841 tgatgtacga atccaaagca ttttcccaaa ccgccatcaa cgccttggag gcatgcgccc
     9901 gcatgatggg tttgaagcct acggcagccg caaaagccaa ggtcttggag ctgggctgtt
     9961 cgatgggcag aaacatcatc acgcaggcac tttattaccc ggatgcggaa tttgtcggta
    10021 tcgacttgtc cggcaggcag gttgcgcagg gcaacgccat cattgaaaaa atgggcttgg
    10081 aaaatgtgcg tctggaagaa aaagatattt tgaccgtcga tgaatcattc gggaagttcg
    10141 actatatcat cgtccacggc atttggtcgc gggtgcccga cgcagttaaa gacaaaatct
    10201 tttcgatttg ccggaacaac ctgaccgaat acggcattgc ctatatttca tacaatgttt
    10261 accccggttg gaagcggcag gagcagttgc gcgatattat gcagtttgcc ggcagggatg
    10321 cgcttggaga acccttggaa gcaaggacgc ggaaaggctt ggatgcgatc aaggcgctgg
    10381 cggaaatttt ggaaaacgac aagggcttgg gcggcggcaa acttccggcg attcaaaaaa
    10441 tattgaatca caatacttat tatgtcgcac acgaatatat ggaaatattc aacaacccga
    10501 tttacgttaa cggtttcatt gaatgggcaa accgccaccg gctggcgtat atcggggata
    10561 ccgatttgca tgtgtctttt gtttcttgga tggcggagca tacgcgggag cggattctgg
    10621 cattggcagg gggtgattat attgccaaag aattttacag cgatatttta tccgaccgcc
    10681 aattccaccg ttcgctttta tgccgtgagg aagtcgggga tactgtcagg cgtgatgagt
    10741 cggttgccgt cgaagtgata gaaagtttga atttccgccc ggcaagaggg gaaacaatca
    10801 actttgatga aaacgatacc ctgctttcgg gcatacgcga tgtgatgaaa accggagagg
    10861 cgtttaaaac ggaagatgtt gcggaaaatc ttgcccgccg atttcccggt ctggaatttg
    10921 accgcatgaa aatcaattcc cagcttttat tgcaaaccat tctcgggcgt ttttctgttt
    10981 catcagacaa tgcgggcaaa ccattttttg aagaccataa aacctatgtg ccggcgcgct
    11041 ttacaaacta tgccgccgcc tttgtggaac acggtgcggg agcgtttgtc cggcctgcca
    11101 accgttacaa cgaaagcacg ccctcattcg gatacgggca tttgtacatt atgcggcaat
    11161 tgtcgcggcc gacgagcaaa caggcattga ttgaaacggt tgccgaaaac ttgaacatcg
    11221 tcagcgccac gcccgacggt ttgacattcc atccgcctgc cgaagtgtat gtggaagaaa
    11281 tattggcaga cttggcagac aggcattttc tcgtttcggc ggattgacgg gcaagtcccg
    11341 acggcaatcc gtccgatata ttttcagaca ggaaagacac acactgcgaa aaacggtaca
    11401 cgacacatat gaaccagaca tttaccttgc ccgatacgcg cccgtatccc caaaatccga
    11461 ttaaaaacca cctgctgctc aatgcctacc agttggcgca caattcgtca caggcttcgc
    11521 gcaaactctc gtccgggcag cttcaaaccg aaatcagggg gatgcttgag caaaaccact
    11581 atatcaacct ttccctcgcg ctgacgatgt cgcccgatgc cggaacttat gccgcgctgc
    11641 tttccagtgt gaacgcggta ctcgattgcg agaaagaagg cgaagtgcag tggttcgccc
    11701 tgccggtcgt gctggtgtcc ggctgcaaga aagaacgggc aatcgagatg aagctgccga
    11761 cggaggcatt gtttgcctgc ctgcaaaact atccgcacct gcgcgcgttg acgcaagaga
    11821 cacaatggct gccttatctt gtgcattctt ccgatttgag tgcggtcgcg ccggatgagt
    11881 ggtggcgtgc caaacaaaat accgaagcgg cggcgcaaca cttacgccgt ttcgccccgc
    11941 gccctttgct gttgcccgaa gggcagtccg tccacgtcgt ttacgtgctg ggtttcggca
    12001 gcggcaaagt tcaggcggca ttggggcaga accttcttca ggcaggcttg cccttaatgc
    12061 aggtatggca ggaaaatctt gcatcggaag gcattacgct gtttgccaat ccgctttccc
    12121 ccgatacccc ggcgcgcgcg ctttcagacg gcagccacac gcgccaacgt atggcgatgg
    12181 atgtttttgc ggcaaacgcg atacgcgccg tccggatgca gggtccgcgc gtcggcgtgg
    12241 tcgctgcggc aaaggcgggc ggacagattc tattcggatt taatgcgacc gacggcgcgt
    12301 ttgaagtcgt gccgcaggtg ttttgctggc agctttcctt taccgataac attgccgtca
    12361 tccagcagaa tttcctcgac ctgatggcag aatgccgtgt agaacacgtt tacctgctgc
    12421 acaatccctt gggcgaacag gaaaacatcc cgagctatgc ggaagcgttg aaacgggaag
    12481 ggcgcaatcc gtttttcagc gcatagtgat ttgtagtagt gataggcttt ctcatttcta
    12541 taataaatca aaataaagag cacaacactt tttcattctg tgttgtgcct tgagtgaaac
    12601 gaaaggatga attataaata tgaagattgt aattgcaccg gattcgttta aggaaagctt
    12661 gacagctcaa caggtagctg aagcaataaa aagaggcttc caacaatcga tagcagatgt
    12721 ggaatgtctc ctctgtcctg tcggtgatgg gggagaaggc actgtagatg ctatccgaca
    12781 ttctcttgac ctagaagaaa aatgtctcca agtgacagga ccttttggac aaaaagaagt
    12841 catgcgctat tttcaaaaag aacaactagc cctattcgaa gttgctgatt tggttggtct
    12901 tggaaaaatc ccgctagaga aacgaaatcc attacaaatc caaactcgtg gtattggaga
    12961 attgattcgc cacctcatta gtcaagaaat taaagaaatc tatattggcg ttggcggtac
    13021 ggccggtaat gatggaggta ttgggattgc tgctggttta ggttatcaat tttatgatga
    13081 ggatggaaat gccttacccg cttgcggtca atccttacta aacccagctt ctgtttcaat
    13141 agaaaatcgc tatgaaattc ctgaagatgt tcacattcgt attttagcag atgttgtgag
    13201 tcccttatgt ggacaccaag gtgcgaccta tacgtttggc aagcaaaaag ggctggattc
    13261 tactatgttt gaggccgtag atcaggcaat acaagatttt tatgaaaaag tcccccctgc
    13321 aacattaaaa cttaaaggag caggagctgg tggaggcatc gctggtggtt tgtgtgcctt
    13381 tgctcaggca agtatcgtat ctggaattga cacctgcctg gacttgattg actttgataa
    13441 gaaagtgtca gatgttgact tggttatcgt tggtaaagga agactagatc gtcaaagttt
    13501 agcagggaaa gcgcctattg gtgtagcaaa aagaacccct gtcggagttc ctgttgtcgc
    13561 tatttgcggc agtttcgctg aagatttgcc ttccctacca tttgaaaata tccaagctgc
    13621 cttttctatt ttggagaaaa gtgaaccttt agaagatagt ttgaaaaatg ccagtcttta
    13681 tttggagcat acggcttcta atattggaca tttattaaat atgcctaaga tttagccaag
    13741 ccatttcttc cagatggatg ttttggcagg ttcttcctta tgcggttacg attaaccggc
    13801 cgatgccgtc tgaaagggtt tccgcttcag acggcatcgc gttggaacag ggatgggata
    13861 tgaaaaaacc gaaaatcctt tttgtctgcc tcggcaacat ctgccgttcg ccgatggcgg
    13921 aatacatttt gcgccgccgt gccgccgaag cgggcattcc cctcgaaacg gacagcgcag
    13981 ggacatcggg ctggcacgac ggcgaggata tgcaccgtga aacggcaaag atattgaaaa
    14041 aatacggcat tgacgcttca ggctttacca gccgcaaaat ccgccaaagc gatgcgacgg
    14101 cgtttgacta catcatcgct atggacggca agaatttgtc cgaattggaa aaaactttcg
    14161 gcaggcggcc ggaaaaaata ttcaagctga ccgacctgat acccgaaagc ggttacgacc
    14221 atgtccccga tccgtggtac atgggtgatt ttgaagaaac atacaggctt gcggatgcag
    14281 gctgtcgggc attattggaa aagatttcca aataaagcaa ttgaatacaa acacaaatat
    14341 aaaaccgcct cctgtcgtat cggtcgttcg gacggcataa acagggaatt tatgaaaaca
    14401 aatttcaaac agaaaattat cgaacaggca cgcagagagg acttgcaggt aaccgctttg
    14461 cgcgagcagg tattagatat tgtcttgcag caaagcggcg tgattaaagc ctacaatgtt
    14521 ttgtcacaga tgcagcagca gagcgaaggc gtgcttgcac cgcctaccgc ctaccgtgcc
    14581 cttgattttt gggcggatca gggcgttttg cacaaagtgg cggcggtcaa cggctatatt
    14641 ttgtgcagcc acgcgcagca cgagtgcaac gaccattgcc acgaccacga agaagccgaa
    14701 gcgcaccaca gcgcgtttat tttggtctgc accgaatgcg gcgcggcgga cgagcaaacc
    14761 ctcagccacg agtgggcggc actgcgtgca ggcgttgccg aaagcggttt tgcgctgaaa
    14821 gaagaacacg ttgttttaac tggaatctgt aaaaaatgtc agaagtgaaa aaaaccaaag
    14881 tccacctgat ttcaggtttt ctgggaacag gcaaaaccac cgcgctcaaa agcctgatgg
    14941 cgcaaaaaga cccgaacgaa aaatgggtca tcatcgtcaa cgagttcggc gaaatcggca
    15001 ttgacggcgc ggtattgagc gacaacggca tccctgtggc agaaatcgcc ggcggctgtt
    15061 tatgctgcac cgccggcccg caaatgggcg taaccgtgca gaaaatgctg cgcgacgcca
    15121 aacccgaccg cctgatgatt gaggcaagcg gactggcgca cgccgccagc gtcatcgacg
    15181 aattgaaagc caaacctctg gacggtcttt tggaaatcgg cgccgtcttt accgtcgtcg
    15241 atccgcgcca gttcatcaat cccgattacg cgcaacaggc gttgtataaa gaccaaatcg
    15301 gcatttgcga cgtattggcc gccggcaaaa ccgatttatg cacccccgaa cagcttgccg
    15361 aatttcatga caaagcggca aaactgttcc cacccaaagc caaagtggtc gaagtccaaa
    15421 acgcacaact cgatatccaa tggctcgata ttcccgtagt tgaaaaatca cgctaccgcc
    15481 tcaaagccct gccggacaac accatgggct tccagtcgca aggtttcaca ttccccgccg
    15541 gacgcgattt cgacggcgaa aaattgacca acttcttcaa tgatttgccc aaaatgaccg
    15601 aaggactcgt ccgcgccaaa ggcgtgttcc aagtattggg aacgtgggtg tggcttaact
    15661 gggtggacgg gcagtggggc gcgaaccaag tgtcttggcg gcgcgattcg cgtttcgagc
    15721 tgattgccaa atcgtttgac gcggatttaa tcgaacaaaa acttaaagac gcattggaat
    15781 agcgttcacg cgctttcaga cggcatatga aatgaaaatg ccgtctgaag gctttaggag
    15841 gacgggatga aacccaaatt caaaaccgtt ttaaccgcgc tgcttttggc ggtttccctg
    15901 ccgtctatgg cggcaacccg cgtcctgatg gaaaccgata tgggcaatat ccgtttggtt
    15961 ttggacgaat ccaaagcctc caaaaccgtt gccaatttcg tgcgctatgc ccgaaaaggc
    16021 ttttacgaca acacgatttt ccaccgcgtc atcggcggct tcgtcatcca aggcggcgga
    16081 ttgaccgagg acttggtgca aaaggcaacc gataaggccg ttgccaacga atccggcaac
    16141 ggcttgaaaa acaccgtcgg caccatcgca atggcgcgga cggcagaccc cgattccgcc
    16201 gccgcccaat tctttatcaa tctggcggac aacggttcgc tcgactacaa aaacggacaa
    16261 tacggctaca ccgttttcgg cagggtcgaa agcggaatgg acaccgtttc caaaatcgcc
    16321 cgcgtcaaaa ccgccacgcg cggcttttat caaaacgtac ccgtacagcc cgtcaaaatc
    16381 cgtcgcgttg ttgtcgggca gtaacacgca gacagacgtt cagacggcgt cgcccgtttc
    16441 ccaaaaaacg ccgtttaaag taaaaaaata ttttaaaaca gacagttgat attgacaaat
    16501 tcaaaccgag gattttaaaa tgctgccaac ccaacccaac ccaacccaac ccaagccctg
    16561 atctaagcga cacagccggg gcgaacactg aggcaatcta cacttcagac ggcattaccg
    16621 ccaactctac ccaactcgaa cagctcaaaa aactgtttcc cgcctgtttt gacgcagacg
    16681 gaaatttcct tatcgacaga ttacaagccg aaattgcgcc acagaccgac atcggacgcg
    16741 aattttacga aatgaactgg ctgggcaaat catatgcccg cctgcttcgc aacctgccgc
    16801 ccgaaaccct gataagtgaa gacaaaacgc acaacgccaa gcccgaaaat gcaggcagcc
    16861 aaaatctgct gattcgcggc gataatctgg aagtgttgaa acacttaaaa aacgcctacg
    16921 caaacagcgt gaagatgatt tacatcgacc cgccctacaa caccggatcg gacggctttg
    16981 tctatcagga cgaccgcaaa ttcacacccg ccgaacttgc cctcctggcg aatattgacg
    17041 aagacgaagc cgcgcggatt ttagatttca ccgacaaagg ctcaaactcg cacagcgcgt
    17101 ggctgacctt tatgtatccg cgcctgtata tcgcccgcga actgttaaag gacgacggcg
    17161 tgatttttat ctcgattgac gataacgaag cggcgcagtt gaaattgttg tgtgatgaag
    17221 tgtttgggga agggaatttt gttgcacaat tgccttggcg aaaaagaaca gctaaatcag
    17281 atgtgccttt tggtatttcg caggattatg aatggatatt tgtattcgca aaatcttgcc
    17341 aatttattgc agcaactaaa ggcaaggaac gacgctatta tgagactgat gatttccccg
    17401 atcgtccttg gcgtacccac gacttaacga aacaaacaac tgcggcggaa aggccaaata
    17461 gttttttcac aatggttgat cccaagacag gaaaaaaata tccagcaaat ccaaatgcaa
    17521 cttggcgtgt aaccaaagat acatttcaag attattataa taaaggaaaa atcgttttcc
    17581 ctgatgatta tgatttctta aatatcagca atccagttat gcgttacttt aaagatgacg
    17641 acatgaaaaa agctggcgag gattttggca aagtagctgt aagcagtaga ttacctgaaa
    17701 atgttggtac attagccgat gcagtagccg aatatttggc tatttttagt aggacgctac
    17761 ctgaaaatat cggaatgact aaagaaggca caaaagaaat cacagattta tttggtagca
    17821 aaatatttac cttccccaag cctagtcaat tgattaaatt tttagtttca ataagttcaa
    17881 agagtaatga cctaatccta gacttcttcg caggcagcgg cacaaccgcc cacgccgtga
    17941 tgcagcttaa cgccgaagga caaaacggta accgccgcta tatctgtgta cagcttcccg
    18001 aaaaaaccgc tgaaaaatcc gaagcccgta aagcaggcta cccgaccatc ttcgacatca
    18061 ccaaagcccg catagaaaaa gccgccgcca aaatccgcgt cgaacatccc gattacacgg
    18121 gcgattcggg cttcaaaatc tttcaaacgg cagacaattt caggcagcat ccagacaagg
    18181 atttttcgcc cgaacaaccg gatttgccgc ttaacgatga attaagcgaa gaacagctgc
    18241 aaacgcttct gaccacctgg acgctgtatg acggggcggc actgaccacg ccggttgagc
    18301 ctgtgcggtt gggcagttat acggcgtatc tgtgcgaaaa acggctgtat ctgcttaaca
    18361 agggttttac ttccgccgat ttgctggcgt ttatcaagaa gctggacgac gatgcggatt
    18421 tcaatcccaa ccgtgtgatt gtattcggca gcaatatggc aagcgccatg cagcacgaac
    18481 tcgaccaggc ggttcgcggt tataccaata aaaaagagat tgagttgaat gtgattatca
    18541 gggtgtaacc ggaatccgaa agtgcaggct gcttttcggc ttggattccc cttgcggggg
    18601 aaggacgata caacaatgac ttcatggaga aacattcatg agcggtttta attacgagaa
    18661 aaaccagccg caccaaatgc gggcggtttc ggcggttttg ggcgtgtttg acggggcaac
    18721 gcccaaatat cggacggcag acgaaaatcc cgaacttttg tttgctgcaa aacaatacgc
    18781 aaacaatatc ctgaaagtgc aaagccaaaa cggtatagac ggccgattcc ccgaccgttc
    18841 ggacgaccaa aatatccttg atatttccat ggaaacgggc acgggcaaaa cctataccta
    18901 cacacaaacc atgttcgagc tgcaccgttg gctgggcgtg ttcaaattta tcgtggtcgt
    18961 gccgactttg tccattaagg cgggaacaca gcagtttttg caaagcaagg ctttggcaga
    19021 gcattttgaa caggatttcg gcggcgatta tgaaggcgta cgcctgaaaa cctatgtggt
    19081 ggaaagcgcg aaaaagaata agggcaaaaa gtccaatgcg cccataacga ttgagcaatt
    19141 tgtcaaagcg gaaaacaaaa aggaaattca tgtgctgctg attaacgcgg gcatggttaa
    19201 ttcgtcgtcc atgaacgata cgggcgacaa ggcattgaag gatttgtttg acaatcccgt
    19261 tgatgcattg gctgccgtgc gcccgtttat gattgtggac gaaccgcata aattcccgac
    19321 ccgagatagc gcgaaaacgt ggggcaatat caaacgctta aaaccgcaat atattttgcg
    19381 ctacggtgca acatttaacg atgaatatta caacttgctt taccgtttga cggcagtaga
    19441 cgcgtttaac gacgggctgg tcaaaggcgt gcgcgtgttt caggaagaaa tgcagggcgg
    19501 catggatgcg gcggtaaaac tggtgtcgtc ggacggcaaa gaagcgaaat ttgaattaaa
    19561 cgaaaaggac aaaaagcaga cgttcaaact ggcaaaaggc gaagatttgg cgcaaatcca
    19621 tccggctatt tcggatttga aaatcgacaa aatgaataaa accgtggtgg tgttaagcaa
    19681 cggcttggag ttgaaaacgg gtgccgtcat caacccttat tcctattcgc aaacggtgca
    19741 ggatgcgatg atgcagcggg cggttgccga acatttcaag ctggaacgcg cgcttttggc
    19801 agaacgcgcg ccacagccca aaatcaagcc gctgacgctg ttttttattg acgatatcgc
    19861 gggctaccgc agcggcaacg agctttcagg cagcctgaaa gataaatttg aaagctggat
    19921 tcgcgcggaa gcggcacgcc gtctgaaaac ggaaagcgac ccgttttacc gcgattacct
    19981 gcaaaagacg ttggacgatg tatccgcctg ccacggcggc tatttttcca aagacaatac
    20041 agacagcgac gatagaatcg agcaggaaat caatgaaatc ctgcacgata aggaaaaact
    20101 gctgtctttg gacaacccgc gccgctttat tttttccaaa tggacgctgc gcgaaggctg
    20161 ggacaatccc aacgttttcc agatttgcaa actgcgttcc agcggcagca cgacttccaa
    20221 gctgcaagaa gtcggacgcg gcctgcgcct gccggtaaac gagctgatgg cgcgggtgcg
    20281 cgatgtaccg tacaaactga attattttgt cgatagcagc gaaaaagact ttgtgaagca
    20341 gcttgtcggc gaaatcaacg acaattcttt tcaggaagaa atctccaaaa agtttaccga
    20401 agagctgaaa caaaaaatat tgcaaaaata ccccgatatc aaaccgctgg tattggtaaa
    20461 ccaactgttt tcagacggca tcattgacga caatgaaaac tttgccgaag acggctatga
    20521 caaattaaaa gccgcctatc ccgaagcctt ccccaaaggt ttggacaaag gcaaagtcag
    20581 caacgccaaa gacgagggca aagacaccat catcatgcgc gaaggcaaat atgaagaact
    20641 caaagccttg tgggagctga ttcaccataa agccgttttg cagtacaaaa tcaaagatga
    20701 agccgaattt gtcgatttat ttaccgccta tttgcgtgaa aacgccgcca aattcccgca
    20761 ggcaggcata tgcacggcgg taaacgaagc ttatatcaac aacgggctta tgctttcccg
    20821 ccgcatagac agtattgaag atgaagattt tatccgtttc aacacaatga cttaccgtga
    20881 gtttctggaa aaactggcac aaacggcaaa aatccagatg cagactttgc atcaggcgtt
    20941 ttaccgcgtc cgcgacgaac tgaacattgg cgattttttg aatatgcaga ccatcgccca
    21001 aatcaaaaac ggcttcaacc ggtttttgct tcatcattcc ttccataaat tcgaactgga
    21061 ttaccggctt gtcggcagca aaatccatcc gaccaaattt accaataaag acggcaaacc
    21121 gcgcgcggtg aaaaaagcag atttgggcag atttgaagat acggagcacc ggcctgccgc
    21181 cggctatctc ttcggcgaga ttttctacga ttcggatata gaacatgaaa atgtcgccaa
    21241 caaccaaatt gaaggcgtaa tcgtatttac caaaataccg agaaactcca tcaaaatccc
    21301 tgttgccggc ggcggcacgt attcgcccga ctttgcctat atcgtgaaaa ccaaaagcgg
    21361 cgagattctg aactttgtga ttgaagccaa agggactgac ggggcggaag atttgcgaaa
    21421 aagcgaagag cggaaaatca aacatgccga aaagctgttt gccgagattt ccaaagaaat
    21481 caaggtggtg ttcaaaacgc agtttgacgg cgagaggata gccgaactga tcgggcaaaa
    21541 tatgccagca ggcgggcatt ctgaaaacgg acactgagaa acaaatgttt ggaagccgtc
    21601 tgcttcggga ttttaaaatg ccgtctgaaa cgccaaacgg gcttcagacg gcatttttga
    21661 cggcggaggt ctatgggccg caggttttcg gcttgttcgc cagaatgttg atgactttgc
    21721 gttcggcttt ttgcggctcg attttgattt cgctctcatc ttcttcgctg ccgtctgaaa
    21781 agcgttcggg cattttttcg ctgtcaaaag ccaaatcgcc gccgtgtttc agattttgac
    21841 cgcgttccaa tccggcaaag tcaaagagtt cggtatcggc aaggtgggaa gggacgacgt
    21901 tttgcagggc ggagaacatc gattcgatac ggccggggaa gcgtttgtcc caatcgcgca
    21961 gcatatcgcc gatgacttgg cgttgtaggt tgggctgcga gccgcagagg ttgcacggga
    22021 tgatggggaa ctgtttcaat tcggcgtatt tgattaagtc tttttctttc acatacgcca
    22081 gcgggcggat gacgatgtgt tcgccgttgt cgctcaccag cttgggcggc atggctttga
    22141 gtttgccgcc gtaaaacata tttaaaaaca tggtggcgag gatgtcgtcg cggtggtgtc
    22201 ccaaggcgat tttggtgcag cccaattctt ttgcggtgcg gtagaggatg ccgcgccgca
    22261 ggcggctgca cagcgaacaa gtcgttttgc cttcgtccaa cacgcgtttg acggtggagt
    22321 aagtgtcttc ttcaacgatt ttgtagggaa cgccgatgct ttcgagatag gtcggcaata
    22381 cttcttcggg gaagcccggc tgtttttggt cgagattgac ggcgaccagt tcaaaatcaa
    22441 tcggcgcgct ggcttggagc cggcgcagga tgtctaacag ggcgtagctg tctttgccgc
    22501 cggagaggca gaccatgatt ttgtcgcccg gctcgatcat gttgaaatcg ttaatcgtgt
    22561 cgccgacggc gtggcgcagg cgtttgctga gtttgttgtt ttcgagttct tgcttggttt
    22621 ttttggacat ggcggtggtt tgaaaaatta gggatgcggc attgtaaccg aaaacagggg
    22681 cggcaatgcc gtctgaaggg cttcagacgg cattagaggg cttattctgc atttttggtt
    22741 ttaaagaaga gatgaaccgc tttgaagata ccgccgtttg ggacggtgag ttctttttgc
    22801 gcggcggaga atttaatcac ggcaagggcg gtaaagtttt ccgaatcttg aacgctgtcg
    22861 agtacgatgc cggcttcttc gccgtccgcc gtcagcagga tgcccgcttc ggctgccgaa
    22921 tttcccgaca ataccgccaa gccgcgtttg acctgccccc gatactgggc gcgcgcgatg
    22981 atttcctgtc ccgggtagca gccttttttg aagtgtacgc cgccgatgat gtgctggttg
    23041 agcatttggg cgacggcggt ttctttggta gccgcgcata tccacggata accgctgcgg
    23101 atttcgtgca gccgccacgc gttttcggcg gcagcgtcat aagggggtag ggcggtttcg
    23161 ggggcgatgt gcaaaatgcc ccgatggtgc aggatgacgg aacagatgcc gtctgagacg
    23221 cattcggagg taaaggcgag actgggttct tgcgcggcaa gcggttcggc ggatgctgcc
    23281 aattccgcgc cgacggcgta atcttcaagg atttcaaaaa cggctttggc gcgcaacaca
    23341 aacatccgca ggcgtttgac cgttgcttca agcaaatctt gcgccataat cagcagcaaa
    23401 tcgtctccac ggttgacgac aatcatattg gcgatgacgc ggcctttggg cgtgttgtaa
    23461 gtcgcgtaac acgcctgccc ggcctgaaga ttattgatgt cgttggaaag ctgtccgtgc
    23521 aggaaggttt ggcggtcttc gccgctgacg cgcgccacgc cgaaaaaggg cagtaaggtt
    23581 ttcatcattt gcgtactctg aaatataagg aaatctgttt atgcagttgc cgcgtctctc
    23641 ttcacggcgg ttattttgat ttcgacgcgc cactccggac gggcgagccg cgcttccacg
    23701 caggcgcggg cgggcgttct gccggcggca acccaagcgt cccacacgcc gttcatttcc
    23761 gcatagtcgc ccatatcacg caggtagatg accgcatcca aaacgtgtgc cttgtccgag
    23821 ccgcattccg ccagccagcg gtcggtttgg gcaagcacgt cggcagtctg ttcggcagcc
    23881 gtttcaccgt tttcgggaac catgccggag aggaaaatca agccgcccgc gcaaaccgct
    23941 tcggaatagc ggggcgttgt gccgaaatac cggatatcca tatcggtttc cttcgataaa
    24001 ggggatatat ggtaacattg cgcttgaccg atttccatgt tttgcatgac gaaaaatgag
    24061 taaacacact tatccgataa cacctgccgt acgcgttttg cgtgaaaacg gcatcgaatt
    24121 tgaacctttt acctatgcct atgaggaaca cggcggcacg gcgcagtttg cccgactgtt
    24181 cggcaaagac gaacacttgg tcattaaaac cattgttttg caagatgaaa acggtaaggg
    24241 gccgattgtc ttgatgcacg gcgacaaaca gatttcaacc cgcaatctgg cgcggcattt
    24301 gggtgcgaaa cacatcgaac ccgccgcgcc tgcgcaggca aacaagtgga cgggctatct
    24361 ggtcggcggc acaacgccgt tcggcatccg gacaaaattg gatatttacg tcgaacagtc
    24421 ggtgatggat ttggaaacca tctatatcaa tggcggaaaa cgcgggttca ttatcggtat
    24481 ccgccccgat gatttaaata ttttgaaccc gaaaacaata caggcagcgg tttgaccggg
    24541 aagtataagg agcaatatgg acaaagattt gtatgccgta ttgggcgtgt cgccgcaggc
    24601 gggggcggac gaaatcaaac gcgcctaccg caagctggcg atgaaatacc atcccgaccg
    24661 caatccgggc aatctgcagg cggaagaaaa gttcaaagaa atccaacggg cttacgatac
    24721 gctttccgac ctgtcaaaac ggacgcaata cgacgcgtcc ttcagacggc atgaggaacg
    24781 cgggcggcag gaagaggcat tccgccgcga acaggcgcgc agggagcagt tttacggcga
    24841 acagatgcgc cgcgaacagg cgttcagacg ggagtttgaa cggcaggctt cacgttcgcg
    24901 ccatgcttac gaaccgtccg gcagcggaag cgggcgcaac tatatcctcg ccgcctacat
    24961 cctgttcggt ttgggtgcaa tcatacagtt catgcccata gtcggcgtga ttctcgccta
    25021 tatgaaacgg aacagtttgg acagcattgt ctatgccgca cataccgaat acctgattaa
    25081 aaccttttgg cgcacatttt ggctttatct tttgggcgcg ctgactgccc gtttcggcat
    25141 cggcgtgctg attattattg caacgaccgt ctggtatttc taccgcatca tcgccggctt
    25201 tatccgcttc aacgacggca gggcggttgc gcccgagaaa tggatataga gcatggccta
    25261 cctgttaatc agcatcgtgt tcagcgtgtc ggtttccatt ttgctgaaaa tggcaaggaa
    25321 gaaaaaaatc gacatcgcgc aggcggtcgc cgtcaattat gtggtcgcgg tcatactgac
    25381 cctgtcggta ttgaagccgg atgtcggcaa tatcggtgca tttttgccga cgtggccgct
    25441 gtttgccgct ttgggcgtgc tgctgccgtc cgtatttgtc attatgggca aatccgtaga
    25501 atccgccggt atcgtcaaat ccgacgcggc gcagcgtttg tcgctgtttt taccgattgt
    25561 tgccgctttg acgctgtttg gcgaaaaact cagtgaaggc aaactgatcg gactgtgcct
    25621 cgcctttgcc gcactgttct gcctgctttg gaaaagcgac agcggcaaaa aatcaggcag
    25681 cgcgtggcgg caggcgttat tgctgctcgg tgtgtgggcg ggttacggca ttatcgacat
    25741 cctgttcaaa cagattgcca aaagcggcac ggcatttgcg ggcaacctgc tggttgcatt
    25801 tgcgctggcg ggtgtgctga tgtttgcctg cctgtttgcc aaatcggtca gatggcgcgt
    25861 tgagagcgtg gtcggcggca tactcttggg cggtttgaat tttatgaaca tcgtaaccta
    25921 catcactgcg caccaaatga tgaaggacag tcccacgctg gtttttgccg gtatgaatat
    25981 cggtgtgatt gttttaggga cgctttcggg cgcattgttc tttaaggaaa aaatcaacac
    26041 aatcaatacg gcaggcattg tactgtcatt atgctccatt gcctgcctgt tctattggac
    26101 agaagttaag gcactgttcg gcatataatc cggctgattg atataacaaa atgccgtctg
    26161 aaacaggaaa atgctgcttc agacggcatt ttgttatacg ggcagatt
//
LOCUS       DAXFWM010000022        24925 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000022 DAXFWM010000000
VERSION     DAXFWM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24925
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.022"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(59..253)
                     /locus_tag="ACFI8B_000907"
     CDS             complement(59..253)
                     /locus_tag="ACFI8B_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC3834167.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="ACFI8B_000908"
     CDS             complement(243..2324)
                     /locus_tag="ACFI8B_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC3834168.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSAFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGILMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2847..3227
                     /locus_tag="ACFI8B_000909"
     CDS             2847..3227
                     /locus_tag="ACFI8B_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC3834169.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3363..3851)
                     /locus_tag="ACFI8B_000910"
                     /pseudo
     CDS             complement(3363..3851)
                     /locus_tag="ACFI8B_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4183..5616
                     /locus_tag="ACFI8B_000911"
     CDS             4183..5616
                     /locus_tag="ACFI8B_000911"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC3834170.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEISAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNP
                     TVRKISFTGSTEVGAKIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYVQSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCIDAG"
     gene            5727..6512
                     /locus_tag="ACFI8B_000912"
     CDS             5727..6512
                     /locus_tag="ACFI8B_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC3834171.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6880..8436
                     /locus_tag="ACFI8B_000913"
     CDS             6880..8436
                     /locus_tag="ACFI8B_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC3834172.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARITGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFAVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8453..9199
                     /gene="surE"
                     /locus_tag="ACFI8B_000914"
     CDS             8453..9199
                     /gene="surE"
                     /locus_tag="ACFI8B_000914"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC3834173.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFYYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9301..10314
                     /locus_tag="ACFI8B_000915"
     CDS             9301..10314
                     /locus_tag="ACFI8B_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC3834174.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQIPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10712..11194)
                     /locus_tag="ACFI8B_000916"
     CDS             complement(10712..11194)
                     /locus_tag="ACFI8B_000916"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC3834175.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11347..11508)
                     /locus_tag="ACFI8B_000917"
     CDS             complement(11347..11508)
                     /locus_tag="ACFI8B_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834176.1"
                     /translation="MSEQPEKHHASPIEDERKNPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11582..12043)
                     /gene="recX"
                     /locus_tag="ACFI8B_000918"
     CDS             complement(11582..12043)
                     /gene="recX"
                     /locus_tag="ACFI8B_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC3834177.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12116..12826)
                     /locus_tag="ACFI8B_000919"
     CDS             complement(12116..12826)
                     /locus_tag="ACFI8B_000919"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC3834178.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLQPQKNKDEE"
     gene            13221..14440
                     /locus_tag="ACFI8B_000920"
                     /pseudo
     CDS             13221..14440
                     /locus_tag="ACFI8B_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14500..15306)
                     /locus_tag="ACFI8B_000921"
     CDS             complement(14500..15306)
                     /locus_tag="ACFI8B_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC3834179.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLITAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15498..15707
                     /locus_tag="ACFI8B_000922"
     CDS             15498..15707
                     /locus_tag="ACFI8B_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC3834180.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15796..17010
                     /locus_tag="ACFI8B_000923"
     CDS             15796..17010
                     /locus_tag="ACFI8B_000923"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC3834181.1"
                     /translation="MDKFPKSAKLDHVCYDIRGTVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17082..19655)
                     /gene="clpB"
                     /locus_tag="ACFI8B_000924"
     CDS             complement(17082..19655)
                     /gene="clpB"
                     /locus_tag="ACFI8B_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC3834182.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPTLARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRIKMELDSKPEQMDKLDRRIIQLKMEKMHVAKESDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAASSSTADIKKQMDDIKVKIEQAKRQGDFARASELEYGELPK
                     LGAQLQAAESNPDGKKQNKLFRTEVGADEVAEIVSRMTGIPVSKMMEGERDKLLKMEE
                     VLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAGFL
                     FDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPHSVILLDEVE
                     KAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAVKE
                     VVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTVSD
                     AALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRLKF
                     A"
     gene            19915..20925
                     /gene="trpS"
                     /locus_tag="ACFI8B_000925"
     CDS             19915..20925
                     /gene="trpS"
                     /locus_tag="ACFI8B_000925"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC3834183.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQVLADGLAWGEAK
                     KLLAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            21006..21551
                     /locus_tag="ACFI8B_000926"
     CDS             21006..21551
                     /locus_tag="ACFI8B_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834184.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21868..22212
                     /locus_tag="ACFI8B_000927"
     CDS             21868..22212
                     /locus_tag="ACFI8B_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834185.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAAEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(22422..23930)
                     /locus_tag="ACFI8B_000928"
     CDS             complement(22422..23930)
                     /locus_tag="ACFI8B_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC3834186.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24093..24860)
                     /locus_tag="ACFI8B_000929"
     CDS             complement(24093..24860)
                     /locus_tag="ACFI8B_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3834187.1"
                     /translation="MPQKAHGLRYGLGVSREINAGGHHFLYGGISRGVHYWDNKDFSE
                     QSLRLSFGHQNRSVTRSFGTVPFVGQNLLGGSRYNFAGGFNADFSRRLGERWRLTLNA
                     GNMWKHYQEDRTAARYGSHMPLAGATLMYSAPKDRLLYGGADWPHDMTKEAEQASVRK
                     GLRVGAVKTFDGGLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSLRHDKISWK
                     GFTPQLNFRYLKIDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         5677 a         6272 c         6953 g         6023 t
ORIGIN      
        1 ccttattata aaaatgccgt ttgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caatataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggcgg agagatagtc gcgcggggtc
     1501 agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgccgaa
     2521 tagaatttta acgcaaatta gcacactgat agcggttttt acttggaatt tggaaaaatt
     2581 tacattcctc cgggcgggca ggcaggttca gacggcatcg tcaggcaaaa ggcggtgtcg
     2641 gaagacgggt aaagaagggg cgcgcaatcc ggattattga tttatcgtgt ttccttttcg
     2701 gttgaacctc agccctttgg ggcggtaaaa tcagacttta tttgggaggg gcgcaacccc
     2761 ttccgaatca ggacggcaca tagggcggtg ctttaatgtg tcgtcctgtg tgttgaaaca
     2821 tattttcttt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtatcgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aagcgtatga tggcggcgga cgcgggtata
     2941 tgccgcctgt tcaaaaccaa gccggcccgg acgattttcg agcgttttca tgcgagaacg
     3001 gtttgtctgt gcgcgtccgc aatttggacg gcggcaaaat cgcgttgcgg ctggacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggcga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacggaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttatgc cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gtttcgttct gggtttatcc
     3361 tgtcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccggctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccccgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata ggttggcaac ccaatcctca
     3601 tagcgttgcc gaaatttttc ggtatcaaaa aaatcttggt cttccgaact gtcataaacg
     3661 aaagtcctgc tgtttgccaa cgcttgcaag accgtcgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaacccc aaacctgaac
     3781 atgtttgaac aatcagagct tcttcattag cataaaaaac tgcccaataa ttgatcctgt
     3841 ttaaaaatca ttatttaatc tccgtaattt tgactgtaat gttttgactt ttgccatact
     3901 ctaccacgcg ttgtaactgc aatctttgct tcttatatat tgtaccctat caaagggctg
     3961 cattactttt ctttctaaag attactatcg cataaaaagt atttttctta aacgacaggg
     4021 atgcgcccgt gccatattca aacaccgccc ggatgttgcg ctgcccgatc ggatgcttca
     4081 gacggcatcg gaagggtttg cagttttgga atatgaagat gataatgtcc gcgagattga
     4141 cggcatttga aagcggttat cccgtatacc ggaggacacg aaatgaacga atattcccaa
     4201 ttaatcaagc atcccgatat ttccctttcc ccgatttcag acggcatcgg ggtcggcaat
     4261 ccggcgacgg gcgaaatttc ggcgtatgtc cgcaacacgg gttcggacaa gctgaaaaac
     4321 ctgattcaaa aggcggcggc agcgcaaaaa ttatgggcgg cgaaaaccgc cttggaacgc
     4381 gccgacatat tgtggcgttg gtatttttta gttaaagaaa acaaagaagc actggcgcgt
     4441 ctcatgacga tggagcaggg caaaagcctg accgaggcgc gcggcgaaat cgattatgcg
     4501 gcttcgtttg tgcgctggtt tgccgaagag gcgcggcgga ttgacggcga tgtgctgacg
     4561 agtgtgaaag cgtcgcaaaa actggttgtg ttgaaacagc ccgtcggcgt taccgctgcg
     4621 attacgccgt ggaatttccc gtccgcgatg attgcgcgca aggccgcgcc tgctttggcg
     4681 gtgggctgcg cgatgatagt caaacctgca tcgctcacgc ctttgagtgc gtatgcgttg
     4741 gctgtactgg cctacgaagc gggcgtgccg caggatttgc ttcccgtcgt cagcggccgc
     4801 gcttcggaaa tcagccatga atttgccacg aacccgaccg tgcgcaaaat cagcttcacc
     4861 ggctcgaccg aagtcggcgc gaaaattttt gccggcagcg cggcggacat taaaaaactc
     4921 agtttggaac tgggcggcaa cgcgccgttt atcgtgtttg acgatgccga tttggacaaa
     4981 gccgtcgaag gcgcgctggc gagcaagttc cgcaacagcg gtcagacctg cgtctgcacc
     5041 aaccgcgttt acgtccaatc gggcatttac gacgtatttt gccgcaaatt gagtgaaaag
     5101 gcagccgcgc tcaaattggg cgacggcttg gacgaaggcg tgaaccaagg accgctgatt
     5161 gaggaaaaag cggtagaaaa agtcgaacag cacatcgccg acgcactctc caaaggcgcg
     5221 gtctgtctga ccggcggcaa acgcagcgcg ttgggcggaa cgttttttga gccgactgtc
     5281 ttaagcggcg ttacggcgca aatggcggta gctcgcgagg aaaccttcgg gcctttgtgt
     5341 ccggtgttcc gttttgaaac cgaagccgaa gtcatcgagg ctgcgaacaa tacggaatac
     5401 ggtttggcag cttacctttt cacctccgac accgcccgtc aatggcgcgt cggcgaagtc
     5461 ttggaatacg gcatggtcgg catcaatacg ggcttaatca gtaatgaagc ggcaccgttc
     5521 ggcggggtga aacgcagcgg tttgggacgt gaaggcagca aatacggtgc ggacgaatat
     5581 ctagaattga aatatttgtg cattgatgcc gggtgacgga tgccgtctga atggcagggt
     5641 ttcagacggt gtcgcatttt aagcagtctc tatctgttgt acaatgcgcc ctgtttttac
     5701 ggttattttt gatttgaatg taagatatga tggaaaacgg aaaaacattc ccgagatggc
     5761 gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag cctattggtt gcagggctgg
     5821 cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg acgggcggtc
     5881 tgtcgcttta tcagctggtg gtggcggtcg atatcgtatg cggcccgctg ctgactttaa
     5941 ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc atggtcggca
     6001 tcatccagct ggcggctttg gcgtacggtt tgcacagcgt ttcgctggcg cgtcccgtgg
     6061 tggaagcgtt tgaacaggac cgcatgacca ttgttacggc ggcggaagtc gtggtcgaag
     6121 atttgcacaa agcccccgaa gggctgcaaa gcctgtcgtg gttcggcatc cgccgcattg
     6181 cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg tccctgaaag
     6241 gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta ttccgacaag gaagcagaaa
     6301 aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga tgcgagaaaa acgacggttg
     6361 cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac ctgccgttta
     6421 ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac accaaaggct
     6481 acgcgccgat agacggcttc atcatccctt aaccattggg attccgcccg cactcgaaca
     6541 tccgttcttc gcggcggtag aatcagactg tatttgagag gggcgcaacc cctcaatcag
     6601 ggcaacacat agggagacgc tttatgtgcc gtccgtgtat tgaaacattt caaaataagc
     6661 ccatgccgtc cgaacatcct ttcagacggc atggcgtttt gccattgccc cgataagctg
     6721 ttaaactgtt taaattattt cgaccgaagg tacacgcaca tgaaagaccc cgagcagagc
     6781 agtaagcccg cccgccgttt tttgtgcgta ccgtctgttc aggcagcttg agcgtttcag
     6841 gctgcctttt tgcgtttgaa cttgaggaac acgaaaatca tggatttcag ttggttggca
     6901 gaaccgcata cctggatagg ttttgccacg cttttggtgt tggaagtcgt attggggata
     6961 gacaaccttg tctttgtggc gattttggca aacaaggtcc agcccgcacg gcgcgaccgc
     7021 gcacggatta ccgggctggg gctggcagtc gtcatccgca tcattatgct tgcttttatg
     7081 gcgcatatca tcacgctgac cgaaccgctg ttccaaatcg gcggcctcgc cgtttccggc
     7141 aaggacatga ttatgctcgc gggcggtatt ttcctgcttt acaaagccac taccgaactg
     7201 cacgaacgcc tcgaagggca taaccggttt gccgttgccg acaaccaaaa aaaacacgcg
     7261 ccgttttggg gcgtggtcgc gcaaatcctg atactggatg ccgtgttttc catcgattcg
     7321 gtcatcaccg ccgtggcgat ggtcgatcac atcgtcgtgg cgatgggcgc ggtcgtcgtc
     7381 gcaatggctg tcatgatttc cgccagcaaa ctcttgaccg aatttgtgga caggcatccc
     7441 accgtcgtga tgctctgcct tggttttttg ttgatgatcg gtttcagcct gattgcggaa
     7501 gccttccatt tccacattcc caaaggctac ctctacgccg ccatcggctt ctcgatttta
     7561 atcgaactgt ttaaccagat ttcgcaacgc aacagccgca aaaacgacta catcggcagc
     7621 tcgtggcgca agcgcaccgc cgaaaacgtt ttgggtatga tgggcatacg cgaaagcgtg
     7681 cttgctgacg cgggcggcga atccgtggat gacgcgcatt ttgaagaaaa cgaaaaatcg
     7741 atgatacgca gcgtgctgac gcttgccgaa cgcccgatta tgggggtgat gattccacgc
     7801 cgcgacatcg aacggctgga catttcccaa agccgcgaag aacagtatgc ccaactgcaa
     7861 aacacgcctt acagccgcct gctcgttgtc ggaaaggcgg gcgtggacga accgttgggc
     7921 tacatcaaca aaaaagacct gctgtcccaa ctgctggaaa caggcggtct cgacattcag
     7981 acggcattgc gccagccgct cgtcctgccc gacggcacga ccgccttggg cgcgctcgaa
     8041 ctcttccgcc aaagcagcgc ggattacgct ttggtggtgg acgagttcgg cgcggtgttg
     8101 ggtatggtga cgatgaaaga cctgttggaa gccatcgcag gcgagttccc cgaagaattc
     8161 gagcgcgaag aagaacccgc cgttcagggg aatcccgatg aaagcctgac ggtggaaggc
     8221 gcgttggaat atgtggagct ggcatcccaa ctcaacctgc cgcagcagga ggaagatacc
     8281 gatttccata ccgttgccgg gctgattatg gaagaattgc aaaccatccc cgatgtcggc
     8341 gattttgccg attttcacgg ctggcggttt gaagtggtcg aaaaagaagg gcagcgcatc
     8401 gagcgggtca aaatcaccaa attgcccgaa gaataagcat ccaggataaa aaatgaacgt
     8461 tttgatttcc aacgacgacg gctatctggc agaaggcatt gccattctgg cgcgggttgc
     8521 gtcggaattt gccaatgtca gggtggtcgc gcccgagcgg gacaggagcg gggtcagcaa
     8581 ttcgctgacg ctcgaccgtc ctttgcagct gaaacaggcg caaaacggct tctactacgt
     8641 caacggcacg ccgaccgact gcatccatgt cggccagttt gccctgcccg attttaagcc
     8701 cgatgtcgtg ttttcaggta tcaaccgtgg cgcaaatatg ggcgacgata cgctttattc
     8761 ggggacggtt gcggcggcga ccgaagccta tctgatgggt atgcctgctg tggcgttttc
     8821 tttaaacgat gcttccggac gctattgggc aaccgcagaa aaggcactgt ggacattgtt
     8881 ggcgcatttt tttaaaaaac cgccgtccgc gcccgtcttg tggaatgtca acatccctgc
     8941 tgtcgcaccg gaagatgtca ggggaatcaa gattacccgt ttgggcagac ggcatcatga
     9001 gcagaacatc gtcccgtccc gcaatccgcg cggcgaacag atttattgga tagggccggt
     9061 cggcgaagtt tccgatcggg aagaggggac ggattttggt gaatgcggcg caggttttat
     9121 taccgtaacg ccgctgcaaa tcgatttgac cgcctatccg gacatggcgg agacggcggc
     9181 cttttggcat acggactgac cgtttttcaa ataccgtcgg gttttgcgct tgagaagaac
     9241 gcgcattttt attatgatat accgcattca ggctatatat cggaacatga aggttatctt
     9301 atgttgaaac aaacgacact tttggcagct tgtaccgccg ttgccgctct gttgggcggt
     9361 tgcgccaccc aacagcctgc tcctgtcatt gcaggcaatt caggtatgca gaccgtatcg
     9421 tctgcgccgg tttacaatcc ttatggcgca acgccgtaca atgccgctcc tgccgccaac
     9481 gatgcgccgt atgtgccgcc cgtgcaaact gcgccggttt attcgcctcc tgcttatgtt
     9541 ccgccgtctg cacctgccgt ttcgggtaca tatgttcctt cttacgcacc cgtcgacatc
     9601 aacgcggcga cgcatactat tgtgcgtggc gacacggtgt acaacatttc caaacgctac
     9661 catatctctc aagacgattt ccgtgcgtgg aacggcatga ccgacaatac gttgagcatc
     9721 ggtcagattg ttaaagtcaa accggcagga tatgccgcac cgaaaaccgc agccgtagaa
     9781 agcaggcccg ccgtaccggc tgccgcgcaa atccctgtga aacccgccgc gcaaccgccc
     9841 gttcagtccg cgccgcaacc tgccgcgccc gctgcggaaa ataaagcggt tcccgccccc
     9901 gcgcccgccc cgcaatctcc tgccgcttcg ccttccggca cgcgttcggt cggcggcatt
     9961 gtttggcagc gtccgaccca aggtaaagtg gttgccgatt tcggcggcgg caacaagggt
    10021 gtcgatattg ccggcaatgc cggacaaccc gttttggcgg cggctgacgg caaagtggtt
    10081 tatgccggtt caggtttgag gggatacgga aacttggtca tcatccagca caattcctct
    10141 ttcctgaccg cgtacgggca caaccaaaaa ttgctggtcg gcgaaggtca gcaggtcaaa
    10201 cgcggtcagc aggttgcttt gatgggcaat accgatgctt ccagaacgca gcttcatttc
    10261 gaggtgcgtc aaaacggcaa accggttaac ccgaacagct atatcgcgtt ctgactttcc
    10321 gtttgcaatc aggttaaaaa tgccgtctga aaggtcttca gacggcattt ttgttttggc
    10381 ggctgtgttt tcaaatcatt ccgaagggac ggggcgtata aatttgtttg actgtgtttg
    10441 ggtatttgct tgatttcatc cgtttgcaat ggaaaagccg cttatttccg ttcggagggc
    10501 ggttttatcg gataaaaggc attttgtccg actgattagg gctgcatcaa gggaatatga
    10561 aaacacaacc gcttagtctc acggctgtgt tccaaaatgg cgttgaaaaa cggatgagcc
    10621 agaatcagcc gattttactt caataaatca aaccattaca ccgaatgtcg gaacaatgct
    10681 tgtcctgtct ttcagacggc ataggccgcc gtcagcagcc gcaggacacg tctccggagg
    10741 cttggaggct gatttctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
    10801 tcaggatttc cagcggaggg acggggacgg gtttgccgtc tttgaccgca accatcgtga
    10861 agtagcagct gttggtatgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
    10921 tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag gtaaccaaat
    10981 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgaaagggta acgcaataat
    11041 tgccgctgta acggctggcg caggaatagg caacctggtc gagcaggagc aggagttcgc
    11101 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
    11161 gttcgtggga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt
    11221 tttgaaatgg gtgcgggaaa attttaggga atggggaagt ggaattcaag ctaaaaaaag
    11281 ctatggaggg atagtctgcc gaaatgggcc gccgatgccg tctgaagcct tcagacggca
    11341 ttttgttcag gaaagccaaa aacggaaaac caagaaaaac acgagtgcca gcacgcccgc
    11401 ccaaacggcg agcatgaatc cggcaaccgc ccgtcccata cggtaaaccg ggtttttgcg
    11461 ttcgtcttcg atgggggagg cgtggtgttt ttcgggttgt tcggacattt tgttctcaat
    11521 cggaaaagtt ggaaacgggc gtaaggttac gctgccgcgc aaaaagattt aagaaggaag
    11581 gtcagcagct gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
    11641 cggcatcaaa accgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttgaggttgg
    11701 cggcgggatg tttgaatttt ttacgcaaca cggctatggc ggcttgcttt tcgcttgagc
    11761 ggtcgggaag caggttgcgg ctggtttttt catctatgcc ctgttgcgcc aaagcctgtt
    11821 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcgtagcgca
    11881 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
    11941 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct tggcgcgaga
    12001 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcac
    12061 ggaaagattc agacggtatg ggaaacggat tccgatgccg tctgaacaga ttgttttatt
    12121 cttcgtcttt gtttttctgc ggttgcaggt tttcgtaaat ttcgggcagc gcgccgataa
    12181 tccggtcggg gcgggtcgta tcgtcttgcg agagcagcgt catatcgccg taaccgaagg
    12241 taacgccgac actcaggcag ccggcggctt tggcggcgat gatgtcgttg cgcgagtcgc
    12301 cgaccataag catattggcg gcatcgatac ccaaaacttc ggcggcgtgc cgcagcggca
    12361 gcgggctggg ttttttttcg ggcaggctgt ctccgccgag tatcaggctg aagtagtcgg
    12421 cgagtcccag ttgttttaga agctcggcgg caaggatttc gtttttgttg gtaatgatta
    12481 ccagcggaat gcccaaggat ttgagcaatg ccagcccggc ttcggtttcg ggataggggc
    12541 gggtgaagac gctcaaatgg tcgcggtagt atttcatata ggacacgaaa cctttttccc
    12601 acagttcgga atcggcttcg cggtcgcggt cgttggtgag gacgcggtga accagtttgc
    12661 cgatgccgtc gccgacgtag ctttcgacca ctttggcggg cagcggtttc ataccgagtt
    12721 gttccaacat cgcttctgcg gcggcggcaa ggtcggggac ggaatcgcac agtgtgccgt
    12781 ccaaatcgaa ggcgacggct tggacgtgtt cgatggctgc attcataatc cggtttcctg
    12841 tcgggttgaa agggtttatt ttaacatcgt ttgcacaggg cttctaatgt atagattatt
    12901 aacaatcgca acaggttttt cgggcggtgt tttcggattt tatggcgggg tctggcaagg
    12961 gtttgaaatc ccggcgatgt tccaattaaa tttttaaaaa ataaaatgat gactatatct
    13021 ttatttaatt ggacatttgc ttttcggatg cgcgctgctt ttttcagacg gatgctgtgc
    13081 ggcttgaaaa ataaaatcat atatagatta ataaaatcgg gcttatgtcg actacatgct
    13141 attacgtttt gtcgggaaat tggttatagt aatgagcgaa atgcacgccc ctgaaaatct
    13201 ttttgcgaaa aggaagcaaa atgtctgatg ccgttgcaaa agaaaccctc aatccgtttg
    13261 aaatcgcgcg aaaacaggtc aaaaccgcct gcgaccggct gaaaaccgat ccggccgttt
    13321 atgaaatcct gaagagtccg acccgcgtgt tggaagtcaa actcgacgac ggcacggtca
    13381 aaacctttac cggttaccgt tcgcgtgcgt tcccatcccg gtgtgaatct ggacgaagtc
    13441 aaggcactgt cgatttggat gaccatcaaa tgctgcgttg caggcattcc ttacggcggc
    13501 ggcaaaggcg gcgttacctt ggatccgcgc gattattccg aggcggaact ggaacgcatc
    13561 gcccgagctt atgccgaagc gattgccccg ctgatcggcg aaaaaaatcg atattcccgc
    13621 ccccgatgtg aacaccaacg gcaaaattat gtcgtggatg gtggatgcct atgaaaacgt
    13681 ggtgaaacat tccgcgccgg gcgtatttac cggcaaaccg gttgagttcg gcggctcttt
    13741 ggcgcgtacc gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ctttggaaaa
    13801 actgggcaaa gatgtgaaag gcgcaaccta cgccattcaa ggtttcggca atgtgggcta
    13861 ccacaccggc tattacgcgc atcaatccgg cgcgaaagtc gttaccgtat ctactgttga
    13921 cgttgctatc tacaatgaaa acggcttgga tatggaagcg ctgtttaaag agttccaaga
    13981 aaaaggcttc atcaccaaca aagccggtta cggcaaagaa attaccaacg ccgaactttt
    14041 ggctttggat atggacgtac tcgccccttg cgcattggaa aaccagctga cttccgaaaa
    14101 cgccggtaaa gtccgcgcga aaatcgtggt tgaaggcgca aacggcccga ctacgcccga
    14161 agccgacgtt atcctgcatc aaaacggcgt attggtcgtg cccgatattt tggcaaactg
    14221 cggcggcgtg gtcgtttcct atttcgagtg ggtgcaaaac ctgcaaggct attattggga
    14281 gtttgacgaa gttcaggaaa aagaaatcgt cgtcctgcgc cgcgcgttcc gcgatatttg
    14341 gaacttggcg caagagtatg atgtcgattt gcgtaccgcg tcttatatga tgagtatccg
    14401 ccgtgttgaa aaagcgatga agctgcgcgg ctggtattga tgtttgaaca aaggatgccg
    14461 tctgaaacca tttcagacgg catttttgtt tggacggaat taatggtgag aatggccgat
    14521 ttggaaggtt aaagttgtgt agttcgcctg tttttggcac aggctttgat cggggaaatc
    14581 ggctttgtat tcgacactcg ctttccaaaa gccttggcgc aaggggatga tgtccacttc
    14641 gcctttgccg tcggtggtgt cggagaaggc ttgggcttcg gttttgtgcg ttttgctgcg
    14701 gtcgctggtg tcgaagccgt caaatgtagc ggtaacggtg gcattgggca gcggttcgcc
    14761 acggaacaga acgcggactt tgaagcgttc gcctacgtga atgttggcgg gattgtccag
    14821 cgggacgatt tccaagtttt gtccgaccgg tttggtgatg atggcggtgt ccgcgctttc
    14881 gtgtcccacg ttgacaatgt ttttaccgaa catacgggtt tgttcgcaat agcttgcgtc
    14941 aggcatttct ttgatgccag cctgtttcca gcctgctttg ttttttgacc ggaaagtagg
    15001 ctgatattcg gcggtaatga ggtagctgcc gtctttgacg ggacggttgc tgcggtattg
    15061 gtagttgtat gtgccgcgtt gaatcatgtt ttccttacct ttttcggtaa ccagctgcat
    15121 cggtttgctg aagatgtgca ggcggtcttt ggcgatgggt tcgagttcgg ggaattcgcc
    15181 gtagcccaag tcggctttaa ggtattcgcc gccgtgcgtg tgggcggttt cgacccagac
    15241 gcggtgggcg tgtgcggatg tggcaaatag tgcggaaacg gcgagcagtg tcaatgtttt
    15301 cttcatggtg cagcctttat atcgtgcggg ttaaaaattt tgttatgcta taacaaaata
    15361 tgtatcgtgt gaagacggat ttgccgcctg aaaactgtct ttttgtatcg atttgaattt
    15421 ttcagaaaat gaagcggacg tttgggcgga ggcaacttgt ttgataagat agcaatattt
    15481 caaaacggag aaatatcatg ccttatgtca atattaaagt aaccggcggc aaggaagcac
    15541 cgactgccgc gcaaaaagcg gaactgatcg gcggcgtaat cgaattgctg gcacgcgtgc
    15601 tgggtaaaaa tcccgaaaca acggttgtcg tgattgacga agtggatacc gataactggg
    15661 atataggcgg caaaagcgtc agcgaacggc gcaaagaggg caggtaaaaa gcctgaaaac
    15721 cccgccctga tgctttaaat ttcgcgtgaa aaagagtaca ttcccaccca ttgcccaaaa
    15781 tttacggaac acattatgga taaatttccc aagtctgcaa agctcgacca cgtctgctac
    15841 gacatacgcg ggacggttca caaaaaagcc cttcagttgg aagaggaggg caacaaaatc
    15901 ctcaagctca atatcggcaa ccctgcgccg ttcggctttg aagcacctga tgaaatctta
    15961 gtcgatgtca tccgcaacct gccgacttcg caaggctatt gcgattcgaa ggggttgtat
    16021 tccgcacgca aagccattgt tcactactat cagaacaaag gtttgcgcga tattacggta
    16081 aacgatgtct atatcggcaa cggcgtgtcc gagctgattg cgatgtctat gcaggcgttg
    16141 ctcaacgacg gcgacgaaat cctgattccc gcgcccgact accctttgtg gacggcggcg
    16201 gcaacgcttg cgggcggtac ggtacgccat tatctgtgcg acgaagaaaa cggctggttt
    16261 cccaaccttg ccgatatgga agccaaaatc acgtccaaaa ccaaagccat cgtcgtcatc
    16321 aaccccaata acccgacagg agcggtgtac agcaaggaaa tcctgctgga aattgccgaa
    16381 ctggcgcgca agcatggttt gattattttc gccgacgaga tttacgacaa aatcctttat
    16441 gatggcgcgg ttcactgcca catcgccgcg ctcgcccccg accttttgac ggtaacgttc
    16501 aacggtttgt ccaaagccta ccgtgtagcc gggttccgcc aaggctggat ggtgctaaac
    16561 gggccgaaac atcacgcaaa aggttatatc gagggtttgg atatgctctc gtccatgcgc
    16621 ttgtgtgcca atacgccgat gcagcacgcg attcagacgg cattgggcgg ttatcagagc
    16681 attaacgaat tcatcttgcc cggcggacgg cttttggaac agcgcaacag agcgtgggaa
    16741 ctggtcaacc agattcccgg cgtgtcctgc gtcaaaccga tgggcgcgat gtatatgttc
    16801 ccgaaaatcg ataccgaaat gtaccgcatc cgcgatgata tgaaattcgt ttacgatttg
    16861 ctggtgcgcg aaaaagtcct gctggtacag ggaacggggt ttaattggat caaacccgac
    16921 catttccgca ttgttacgct gccttacgtc catcagattg aagaggcgat gggcaggttg
    16981 gcaagattcc tgcaaaccta ccatcagtag gattctgata aaaaatgccg cctggaacgg
    17041 agattcccgt ttcagacggc attttcaaca gcaggaacaa atcaggcaaa tttcagtctg
    17101 tcgccgtcgg cttctacctt gatttcgctt tcgggcgcat agtttccggc aagcagggct
    17161 ttggccagcg ggttttcgat ttccgattgg atcgcgcgtt tgagcggacg tgcgccgtaa
    17221 atcgggtcga aaccggcttt ggcgatgatg tccagagcgg catcggaaac agtcaggcgc
    17281 aggttttgtt tttccaaacg tttttccaaa cctttgagct ggattttcgc aatactgcgg
    17341 atattggcct gatccaagcc atggaacacg accacttcgt cgatgcggtt gatcatttcc
    17401 ggacggaaat gttctttcac atcctccatc acaacttctt tcaccgcttc gtagtcctgc
    17461 gtgcccattt gttggatgtg ctggctgccg atgttggaag tcatcacaat cacggtattt
    17521 ttaaaatcta ccgtcctgcc ttgtccgtcg gtcaaacgac cgtcatccaa tacttgcagc
    17581 aggatgttga atacgtcggg atgggctttt tccacttcgt ccaaaagaat cacgctgtgc
    17641 ggtttgcggc ggacttgttc ggtcaggtag ccgccctctt cgtatccgac ataacccgga
    17701 ggcgcgccga ttaagcgggc aacggcgtgt ttttccatgt attcggacat gtcgatgcgg
    17761 atgaggtggt cttcgctgtc gaacaggaag cctgccaaag ctttgcacaa ctcggtctta
    17821 cccacaccgg tcgggcctaa gaacaggaag ctgccgtaag gtttgttcgg atcggcaagg
    17881 ccggagcggc tgcggcggat ggcatcggac acggcgcgca cggcttcgtt ttgaccgact
    17941 acgcggcggt gcaacacttc ttccattttc aacaatttat cgcgttcgcc ttccatcatt
    18001 ttggatacgg gaatgccggt catacgggaa acgatttcgg ccacttcatc cgcgccgact
    18061 tcggtacgga aaagtttgtt ttgttttttg ccgtcggggt tgctttccgc tgcctgcaac
    18121 tgtgcgccca atttcggcaa ctcgccgtat tcgagttcgg acgcgcgggc gaagtcgcct
    18181 tgacgtttgg cttgttcgat tttgactttg atgtcgtcca tttgtttctt gatgtcggca
    18241 gtgctggaag acgcggcttt ttcggctttc cagatttcgt ccaaatcggc gtattctttt
    18301 tgcaggccgt cgatttcttc gtctatcagt tccaaacgtt ttttgctggc atcatcgctt
    18361 tctttggcaa cgtgcatttt ttccattttg agctggatga tgcggcggtc gagtttgtcc
    18421 atctgttcgg gtttgctgtc cagctccatt ttgatgcggc tggcagcttc gtcaatcaaa
    18481 tcaatcgcct tgtcgggcag gaagcggtcg gtaatgtagc ggtcgctcaa ctccgctgcg
    18541 gcaacgatag cagggtcggt aatatcgatg ccgtggtgga tttcgtaacg ttcctgcaaa
    18601 ccgcgcagga tagcgatggt gtcttccacg ctgggttcgc cgaccaatac tttttggaaa
    18661 cggcgttcga gtgccgcgtc tttttcgatg tattggcggt attcgtccaa agtagtcgca
    18721 ccgatacagt gcagttcgcc ccgcgccaag gtcggtttca acatattgcc cgcgtccatc
    18781 gcgccgtcgg ttttgcccgc gccgaccaaa gtatggattt catcgatgaa aatcagggtg
    18841 ttgccgtcgt ctttcgccaa atcgttcaat acgcctttca agcgttcttc aaattcgcca
    18901 cggtatttcg caccggcaat caaagccgcc aaatccaaaa ccagcaggcg tttgttacgc
    18961 agggattcag gtacttcgcc gttgacgatg cgttgcgcca aaccttcgac gatggcggtt
    19021 ttacccacac cgggttcgcc aatcagcacg gggttgtttt tggtgcggcg ttgcagcacc
    19081 tgaatcgcgc ggcggatttc atcatcacga ccgataacgg ggtcgagctt accgtctcgg
    19141 gcgcgttggg tcagatcgag cgtgtatttt ttcaaagcat cgcgttggtc ttcggcattc
    19201 gcatcgttca cgttttgtcc gcctcgtacc gcatcgatcg cggcattgat attttgttcg
    19261 gtcgcaccgg cttctttcaa gattttgccg gccgcatcgt tctgctgcac caaggcgagc
    19321 aggaaaagtt cgctggcgat ataggcatcg ccgcgtttgg ttgccgcttt gtccatcagg
    19381 ttcaacaccg cctgcaattc gcggctgggc agaatctcac caccttgtcc ggacactttc
    19441 ggcaggctgt tcaaatgctg ctgcaaacgc tgtttaacct gcggcacgtt cacgcccgca
    19501 tgagccaaga gcgcggcagc tccgctgttt tggtcgtcaa gcagggcttt cagtacgaaa
    19561 cctgcttcca gatagctgct gtccgcagcc aacgccaaac tctgagcttc tgcaagggct
    19621 tgttggaatt tggcggttaa tttgtcgtat cgcattttcg tttccttttc aaaatgtccg
    19681 ctgtcgaagc ctatatgtgc atgactgtgg ataactcaag tgttgttttc tgtttttcta
    19741 tatttaattc gatatattgt tgaatttaaa gtatatagaa atgtataata atatgtataa
    19801 ctatatcttc ttaatatgga aaagtctgtt gtcggctgga tgtatgtggc aaatcgggta
    19861 taatcggcgc atcttttccc tttcagacgg cattgctgcc gtaaggacat ttttatgagc
    19921 aaaaaacgag ttctgaccgg cgtaaccacc accggcaccc cgcatctggg caactacgtc
    19981 ggcgccatcc gccccgccgt ccgcgcggcg caaaaccccg ataccgaatc cttcctcttc
    20041 ctcgccgatt accacggcat catcaaatgc cacgagcagg agatgattca ccaatccacc
    20101 caagccgttg ccgccacttg gcttgcctgc ggactcgacc ccgagcgcac caccttctac
    20161 cgccaaagcg acattcccga agtgatggag ttgaactgga ttctgacctg catcaccgcc
    20221 aagggtttga tgaaccgcgc ccacgcctac aaagccgccg ttcaagccaa tgcggaaaac
    20281 gggcaggaag accccgattt cggtgtggaa atgggtttgt tcagttatcc gattctgatg
    20341 actgccgaca ttttgatgtt caacgccaac gaagtccccg tcgggcgcga ccaaatccaa
    20401 cacgtcgaaa tggcgcgcga cattgccggc cgcttcaacc accgtttcca agaactcttc
    20461 accctgcccg aagtgaaaat cgacgaaaac gtcgaactct tggtcggttt ggacggacgc
    20521 aaaatgtcca aatcctacgg caataccatt ccgctttggg aaaacgacaa aaaaacccaa
    20581 aaatcggtca acaaaatcat caccaacatg aaagagccgg gcgagccgaa acagcccgac
    20641 gaaagccctt tgtttgaaat ctataaagcc ttctccacgc cgtctgaaac ggcggaattt
    20701 acgcaagtgc ttgccgacgg cttggcttgg ggcgaggcga aaaaactttt ggcggcgaaa
    20761 atcaacgccg aactggcaga actgcgcgaa cgctacaacg cattgacctc taatccttcg
    20821 caaatcgaag agattttgca ggcaggcgcg caaaaagcgc gcaaagaagc gcgcgaattg
    20881 ttggataaag tacgcgatgc ggtcggcatc cgcccgttga aataaaccca atgccgtccg
    20941 aacctccgcc tgccacgttt cagacggcat ttcagacggc attttgaaac catcaggagt
    21001 gcggaatgct gaaaatacct tttgccgtgt tgggcggctg cctgctgctt gccgcctgcg
    21061 gcaaatccga aaatacggcg gaacagccgc aaaatgcggc acaaagcgcg ccgaaaccgg
    21121 ttttcaaagt caaatacatc gacaatacgg cgattgccgg tttggctttg ggacaaagta
    21181 gcgaaggcaa aaccaacgac ggcaaaaaac aaatcagtta tccgattaaa ggcttgccgg
    21241 aacaaaacgc cgtccggctg accggaaagc atcccaacga cttggaagcc gtcgtcggca
    21301 aatgtatgga aaccgacgga aaggacgcgc cttcgggctg ggcggaaaac ggcgtgtgcc
    21361 ataccttgtt tgccaaactg gtgggcaata tcgccgaaga cggcggcaaa ctgacggatt
    21421 acctgatttc gcattccgcc ctgcaaccct atcaggcagg caaaagcggc tatgccgccg
    21481 tgcagaacgg acgctatgtg ctggaaatcg acagtgaggg ggcgttttat ttccgccgcc
    21541 gccattattg agttattcgg acatcacgga atatatttgg atttttcaaa ccttgcggaa
    21601 aaagtccgcg ccgccgggaa actcaaaagg aggggatgtg tgttacaatt ttccgaactg
    21661 ttttcataaa atagttttcg gacgtgtttc aatatggcat tgatgccgcc ttgttgttcg
    21721 agaaaaaacc ctttatattt aatataatgg gttttaacta aacgggaaac cgttttctct
    21781 ccggccgatg ggcaaaatca gccgattgat ggaacacggt ccggttttta agcaaaacct
    21841 taatcaacaa ctcaagaagg ataagacatg aaaaaattat tgattgccgc aatgatggcg
    21901 gctgccttgg cagcttgttc gcaagaagcc aaacaggagg ttaaagaagc ggcccaagcc
    21961 gttgagtccg atgttaaaga cactgcggct tctgccgccg agtctgccgc ttctgccgcc
    22021 gaagaagcga aaggccaagt caaagatgct gcggctgatg caaaggcaag tgccgaggaa
    22081 gctgtaactg aagccaaaga cgcggcagcc gaaaccaaag aagcggtaag cgaagcggct
    22141 aaagacactt tgaacaaagc tgccgacgcg gctcaggaag cggcagacaa aatgaaagac
    22201 gccgccaaat aatttgttgc cttggcaaaa atgacgagat gccgcctgcc ggcaacccaa
    22261 atgaaaccgc ctgaagattt tcaggcggtt tttgagttgt atgccccttg ttttttacgc
    22321 ctgcatgacc gtgcagggag gatgtgccgc gcatatcggg atattgcgga cagggaaatg
    22381 ccgtctgaac atcatttaga cggcatttgg tttgtcaagg ttcagacggc ttcaactttg
    22441 aacggcatat tgattttttg ccattggacg ctttcatatt ccaatgcgtc ggcaatcagg
    22501 gggtggcgtt ccagccattt cctgtcaata cgcaggatga agccgcagct ttccgtatcc
    22561 gtacgcaact gcatattttt cgggaaagac aggtcttggc gcgaacggca gaacaaggcg
    22621 gcaaggcgca gggacaaaac ggcataccac aacatttcgt tgccgccgat aatgccgccc
    22681 atttttttca tatcgccgcg atgaccgatg accagttggg caagtatggt ctgttccttg
    22741 cgtgaaaaac ccggcatatc ggcgttttcg aggatgtagg cggaatgctt gtgatagccg
    22801 gtatgggcga tgtccaaacc gatttcatgc agcgcggcgg cgcgtccgag atactgttgc
    22861 cacaaggcaa gttcttgaac cgtaacgttt ttggcatgac agaggctgtc cataaaggtt
    22921 tgcgcggtct cggcggtgcg tttcgcctga ttgaggctga cgtggtagcg gtgttggaac
    22981 tcggcaaccg tttgtccgcg catatcttcg tttaagccgc gcccgattaa atcgtaaaac
    23041 acgccgtcgc gcagggcggc ttcggttacg gtcatcctgt cgagtttcat ttcctcaaac
    23101 gccgccatca tcacggcaag tccgccggca aaaacttcga tgcgttccgg tttcaggttt
    23161 tcaaatttgg cttttttgac cgaaccggct tcgatgatgc gttcggcgag ggcgcgcatt
    23221 cccttgtagg taatgtccgc ctcttggggc atttcggcgg caagcacgtc gcggatggat
    23281 tttgccgaac ccgatgtgcc gacggcgaaa tcccaacctt cgcgcctcat atttttgctg
    23341 atgcgctgga tttcgttgcg ggcggcggaa atggcggctt ggaagtcttt ggcggtgatt
    23401 ttgttttgga agaagcgcag gctgtaggtt acgcagccca agggcaggct ttcggtaatg
    23461 tcgggattca gcgtcgaacc gatgacaaat tctgtcgaac tgccgccgat gtcgataacc
    23521 agcattttgc ccccgcaggg ggggagggtg tggatcacgc cggtataaat cagccgcgcc
    23581 tcttcgcgcc cggcgatgat ttcgatgggg aaacccagtg ccgcttcggc cttgggaagg
    23641 aaatctgcga tgtttttggc gacgcggaat gtattggttg ccacggcgcg cacctgctcg
    23701 gggcggaagc cgcgcaggcg ttcgccgaat tttgccaggc agtccaaagc ctgttcctgc
    23761 gaggatgtgc tgaggttttt ctgttcgtcc agaccggcgg caaaacggac catctgtttg
    23821 aaggaatcga tgacttttaa ttgtccgttg ttgttttcgc aaatctggag gcggaaactg
    23881 ttggaaccca aatcgacgga ggcgaggacg tttgcgggat tggtggtcat ggcggatacc
    23941 ggtgggggaa aaacgcaatg ttactctgac ggcgcaggcg ttgacaataa atgatgcggc
    24001 tgtttttgat tctgctcacg gatgttgccg acggtatgtt tccgacagca aaaaatgccg
    24061 tctgaaaaac gttcggacgg cattttttgc gcttatttga aatccttttc cacgctcatg
    24121 aaaatctgcg tatttttgcg tgtgtaaaaa cttttcatat tgctgtcgat tttcagatag
    24181 cggaaattga gttgcggcgt aaagcccttc caagagattt tgtcgtgccg caacgacagg
    24241 tttgcctgat attcgtggtc tttgcgcggg aagcggtaca caattgtccc gggtgcatca
    24301 aaagtcctgc gggtatagcg caggtttgcc cgcagcccca agccgccgtc gaacgttttg
    24361 accgcgccga cacgcaaacc cttgcggacg gaagcctgtt ccgcctcttt cgtcatgtcg
    24421 tgcggccagt ccgcaccgcc gtaaagcagc cggtctttcg gcgcggaata catcagcgtc
    24481 gcgcccgcca gcggcatatg gctgccgtat cgggcggcgg tgcggtcttc ctgataatgc
    24541 ttccacatat tgcccgcgtt cagcgtcagc cgccagcgtt cgcccaagcg tcgggagaaa
    24601 tcggcattga agccgccggc gaaattgtat cggctgccgc ccaagaggtt ttgcccgaca
    24661 aacggcacgg tgccgaacga gcgcgttacc gaacggtttt gatggccgaa cgacaggcgc
    24721 aggctctgtt cgctgaaatc tttgttatcc caataatgca cgccgcggct gatgccgccg
    24781 tagaggaaat gatgcccgcc cgcattgatt tcgcgcgaca cgcccaagcc gtagcgcaaa
    24841 ccgtgtgcct tttgcggcag gctgtcggcg gttttcgtcc agcttctgcc cgcaaattca
    24901 atcgtttttt cggacgaagc gttgt
//
LOCUS       DAXFWM010000023        24487 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000023 DAXFWM010000000
VERSION     DAXFWM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24487
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.023"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(428..513)
                     /locus_tag="ACFI8B_000930"
     tRNA            complement(428..513)
                     /locus_tag="ACFI8B_000930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Leu,seq:gag)
     gene            complement(527..877)
                     /gene="secG"
                     /locus_tag="ACFI8B_000931"
     CDS             complement(527..877)
                     /gene="secG"
                     /locus_tag="ACFI8B_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC3834188.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(884..1657)
                     /gene="tpiA"
                     /locus_tag="ACFI8B_000932"
     CDS             complement(884..1657)
                     /gene="tpiA"
                     /locus_tag="ACFI8B_000932"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC3834189.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            1816..2343
                     /locus_tag="ACFI8B_000933"
     CDS             1816..2343
                     /locus_tag="ACFI8B_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC3834190.1"
                     /translation="MYANMGDMMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWLWVGFYFVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGEL
                     AKILERRFEASRQAA"
     gene            2446..3102
                     /locus_tag="ACFI8B_000934"
     CDS             2446..3102
                     /locus_tag="ACFI8B_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC3834191.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            3183..3506
                     /locus_tag="ACFI8B_000935"
     CDS             3183..3506
                     /locus_tag="ACFI8B_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC3834192.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(3769..5906)
                     /gene="tdfF"
                     /locus_tag="ACFI8B_000936"
                     /pseudo
     CDS             complement(3769..5906)
                     /gene="tdfF"
                     /locus_tag="ACFI8B_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
     gene            complement(6169..6492)
                     /locus_tag="ACFI8B_000937"
     CDS             complement(6169..6492)
                     /locus_tag="ACFI8B_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC3834193.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
     gene            complement(6525..7496)
                     /locus_tag="ACFI8B_000938"
     CDS             complement(6525..7496)
                     /locus_tag="ACFI8B_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3834194.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            7527..7688
                     /locus_tag="ACFI8B_000939"
     CDS             7527..7688
                     /locus_tag="ACFI8B_000939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834195.1"
                     /translation="MFIIKQTTCRNHAAKRKIPDTAKQIPDTAKQIPDTAKQIPDTAK
                     QIPETEKVV"
     gene            7795..8751
                     /locus_tag="ACFI8B_000940"
     CDS             7795..8751
                     /locus_tag="ACFI8B_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3834196.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            complement(8864..10879)
                     /locus_tag="ACFI8B_000941"
     CDS             complement(8864..10879)
                     /locus_tag="ACFI8B_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC3834197.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(10975..12285)
                     /gene="argA"
                     /locus_tag="ACFI8B_000942"
     CDS             complement(10975..12285)
                     /gene="argA"
                     /locus_tag="ACFI8B_000942"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC3834198.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            complement(12282..12710)
                     /locus_tag="ACFI8B_000943"
     CDS             complement(12282..12710)
                     /locus_tag="ACFI8B_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC3834199.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            complement(12778..13419)
                     /gene="pyrE"
                     /locus_tag="ACFI8B_000944"
     CDS             complement(12778..13419)
                     /gene="pyrE"
                     /locus_tag="ACFI8B_000944"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC3834200.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GKLSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            complement(13481..14218)
                     /locus_tag="ACFI8B_000945"
     CDS             complement(13481..14218)
                     /locus_tag="ACFI8B_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC3834201.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            complement(14212..14652)
                     /gene="rimI"
                     /locus_tag="ACFI8B_000946"
     CDS             complement(14212..14652)
                     /gene="rimI"
                     /locus_tag="ACFI8B_000946"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC3834202.1"
                     /translation="MNIRPASPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            complement(14649..15323)
                     /gene="tsaB"
                     /locus_tag="ACFI8B_000947"
     CDS             complement(14649..15323)
                     /gene="tsaB"
                     /locus_tag="ACFI8B_000947"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC3834203.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGYPATGAAHAELLYVRNKIALTAKEQA
                     ERKART"
     gene            complement(15462..16319)
                     /locus_tag="ACFI8B_000948"
     CDS             complement(15462..16319)
                     /locus_tag="ACFI8B_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC3834204.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGDRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            complement(16458..17522)
                     /gene="fba"
                     /locus_tag="ACFI8B_000949"
     CDS             complement(16458..17522)
                     /gene="fba"
                     /locus_tag="ACFI8B_000949"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC3834205.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            17716..18633
                     /gene="xerC"
                     /locus_tag="ACFI8B_000950"
     CDS             17716..18633
                     /gene="xerC"
                     /locus_tag="ACFI8B_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC3834206.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            18718..20631
                     /gene="dxs"
                     /locus_tag="ACFI8B_000951"
     CDS             18718..20631
                     /gene="dxs"
                     /locus_tag="ACFI8B_000951"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC3834207.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(20696..20860)
                     /locus_tag="ACFI8B_000952"
     CDS             complement(20696..20860)
                     /locus_tag="ACFI8B_000952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834208.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(20878..22206)
                     /gene="miaB"
                     /locus_tag="ACFI8B_000953"
     CDS             complement(20878..22206)
                     /gene="miaB"
                     /locus_tag="ACFI8B_000953"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC3834209.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            22409..22489
                     /locus_tag="ACFI8B_000954"
                     /pseudo
     CDS             22409..22489
                     /locus_tag="ACFI8B_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            22543..22926
                     /locus_tag="ACFI8B_000955"
     CDS             22543..22926
                     /locus_tag="ACFI8B_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC3834210.1"
                     /translation="MYCLRLRRLVLIFVNPLYQTEEGIAQTVVKGKTTRAEVEARFGK
                     RNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCADYDGEGVVRDYRFTHKEEKD
                     ERSVIRDTVGVIRKEAGKSLSQPEK"
     gene            22990..24270
                     /gene="hemL"
                     /locus_tag="ACFI8B_000956"
     CDS             22990..24270
                     /gene="hemL"
                     /locus_tag="ACFI8B_000956"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC3834211.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
BASE COUNT         4742 a         6408 c         7641 g         5696 t
ORIGIN      
        1 gtcctgattt aaatttaatt cactatattt catatactta tttatctaac ttttccccgg
       61 taacaaggta acaactaagg gtatcgcttc ctagtatagg ttttttccaa aaacccaaaa
      121 aaaccgctct gcatcttgga cggtttcccc ctcgttccct acggttcgtc agtattcccc
      181 gcaagctctt ttccgcttgg cattcctgat ttggtggtcg ttcagcttta tttttgcggt
      241 atttttcgga tatcgtaaaa aatcaggtta gttcaattag ggcggattgg acgggattgc
      301 acagtcagga aggggacggc aggataacaa tcagcctgaa atccttgtat tgattgggat
      361 tggttgatgg catcagacag gattaaacga aaaaaaacgc ctagaatttc taagcgtttt
      421 tgtgtgttgg tgccgacagc gagatttgaa ctcgcacagc ctacggccac taccccctca
      481 agatagcgtg tctaccaatt tcaccatgtc ggcatttgaa aaactgttat ttctgctgct
      541 gaggaacagg ggcagaaggt tcggtattgc ttacgggttt gggtgcttgc tgagtctgtc
      601 gtatgttgct gaagtccaaa ccgtgttttg tcgtgtgggt gtgaatatac accatagcca
      661 tgcaggttgc aaagaaaaat gttgctgcaa cggcggtcga gcggctgagg aagttggcgt
      721 tgccggcaga gccgaatacg ccttgcgcgc tgccgcttcc cgatccgaag gtcgcgccgg
      781 catccgcgcc tttgccgtgt tggagcaata ctaacacgat gacggccaaa gcggaaataa
      841 tattaataat ccaaattagg gttttgaagg cttccatatt tttctacgca ttttgtgcgg
      901 cactgatgat ggcggtaaag gagtcgtacg acaatgacgc gccgccgacg agtgcgccgt
      961 ccacataagg tactgcgaag atgtcggccg cgttgtccgc tttcacactt ccgccgtaaa
     1021 ggacgcggat tttaacatcg cttccgcaca aagacaagat ttctttgtag atgaatgcgt
     1081 gcatatcggc aatctgttcg acggtggcga ctttgcctgt gccgattgcc cagacgggtt
     1141 cgtaggcgac ggcgatgttt ttggtatcca gcccttgcag gatggaaagc tgatgggcga
     1201 tgacttcgtg ttctttgccg gtttcgcgct cttcgaggct ttcgccgacg cacaataatg
     1261 ggatgagtcc gacgttgagg acgttttcca ttttgcggcg ttggatttcg tttttttcgc
     1321 cgaaataaag gctgcgttcg gagtgtccga tgaggacgat gtctgtgccg gtgtcggcga
     1381 gcatttcggc ggacacttcg ccggtgtacg cgccgttgtc ggggaagcgg ctcacgtctt
     1441 gggcgcaggt gaggatgcgg ttgtttaaga cgatttgcat ggcgttgtgc agttgcagca
     1501 ggtaaacggt cggggcggcg agtccgatga ggacgcgttc ggcggtgggg aggatgcgga
     1561 agcggtgcat cagtgcgttg ttgttttgga gccggccgtt cattttccag ttgccgatga
     1621 cccatttttg atcccacatt ccgatttggc gatacatctt ttttgctccg tgtcgtgttt
     1681 ttctgtctgc cgcgtgtggc gcgttgcaat gtgaagttta gtggatatgc ggcgggttcg
     1741 caacttgggg cgggcggctg cgggggcggt ttggaatgtt gtttcgggca ggatgtttta
     1801 taatggctgc ctgatatgta tgcaaatatg ggagatatga tgcacgcgct tcatttttcg
     1861 gcttcggaca aggccgcgct ttatcgtgag gtgttgccgc agattgagtc tgtggtggcg
     1921 gacgaggcgg attgggtggc gaatttggcg aacacggcgg cggttttgaa ggaggcgttc
     1981 ggctggttgt gggtgggttt ttattttgtc gatacgcgtt cggacgaatt ggttttggca
     2041 ccgtttcagg gacctttggc gtgtacgcgg attccgttcg ggcgcggggt gtgcggtcag
     2101 gcttgggcga agggtgaaac ggtggttgtt aaggatgtga acgcgcatcc cgaccatatt
     2161 gcctgttcgt ctttgtcgcg ttcggagatt gtcgtgccgc tgttttcaga cggccgctgt
     2221 atcggcgtgt tggacgcgga cagcgaacat ttggcgcagt ttgatgaggc agatgctttg
     2281 tatttgggcg aactggcgaa gattttggag aggcggtttg aggcttcgcg tcaggcggct
     2341 tgagactggc aaaacgggcg ggcttcgcgt gccgaagttg gcgcggcggt agtgtggttt
     2401 tataatgcct gccattgata aaacaattat ttgacggagc actaaatgga ttttgaaaaa
     2461 gcgcggttca atatggtcga acagcagatc cgtccttggg atgtattgga ttttgacgtg
     2521 ttggacgctt tggaggagat tccgcgcgag cttttcgcgg atgagtcttt gcagggtttg
     2581 gcgtatgcgg atatggagct gccgcttgcc aacggtcata agatgctcga gccgaaagtc
     2641 gtggcgcggc tggcgcaggg cttgaagctg acgaaaaacg atacggtttt ggaaatcggc
     2701 acgggttcgg gctatgcgac ggctctgttg gcaaaactgg ctggccgtgt ggtttcggac
     2761 gacatcgatg ccgaacggca aaagcgcgcc aaagcggttt tggacggctt gtctttggaa
     2821 aacatcgatt atgtgcaaaa taacgggttg accgaacttt ccgcaggtgc gccgtttgat
     2881 gcggtttacg tcggcggcgc ggtaaccctt gtgcctgaag tgttaaagga acagttgaag
     2941 gacggcgggc gtatggcggt tattgtggga cgcaggccgg tgcagcgcgc gcttttgatt
     3001 acgcgcaggg gcgatgtgtt tgaagagaag gtgctgttcg atactttggt ggcgcatttg
     3061 gacgacaagg atgcccatcc tttcgacagt tttaattttt gatgttcgga ttgtgatgcc
     3121 gtctgaaagc gggtttgggg cttcagacgg cattttgctt ggtttttttc ggggggtttg
     3181 tgatggatat tgtgcaactt ccgtcggcgg cattgaaggc gtggatggac gaagggcgga
     3241 tgttttgttt gttggacgtg cgtacggatg aagaagcggc ggtttgttcg ctgccaaatg
     3301 cgctgcatat cccgatgaat ctgattccgc tgcggcaaaa cgagttgccg gacgatgtgc
     3361 cgcttgtggt gtattgccat cacggtatcc gcagcctgca tacggcgatg tatttggcgg
     3421 aggcgggttt tgaaaacctg tacaaccttc agggcggcat cgacgcgtgg gcggttgagg
     3481 ttgacgcgga aatggcgcgg tattgaaggc ggcttcagac ggcattcctt aaatgcgtgt
     3541 acctctggtg ttccataaag gtcgtctgaa agtgcagctt ctgcgaagct aaagcgcagt
     3601 atcaacgaag ttaaaatttg cctgaacctt aaaggcagcc tgcaccccaa ttccctcgcc
     3661 ccgtgggaga aggctaggga gagggcggca aactgcaggt ttgcttgggc ggcattttca
     3721 atgtgcaggc tgcttttagc ttcgcagaaa ctccgttttc agacgacctt atttaaaccg
     3781 ataggtaaac gccgcgttca ctgtccgcag tgcgccgtag ctgtggcggt cgggctgggt
     3841 gcggtagtgt ttgttgaaca ggttgtccac gttcagcgac agttcggtgc gcggattgaa
     3901 gcggtaacgc gccatgatgt cggcgacggc gcgggctttg gcggcggggt tggggatgcg
     3961 gagcgcggct gggtcggtgt gggtttcgcc ctgccggcgc acacccgcac cgatggtccg
     4021 gccgctgggg gcttcggggg ctaagtggta ggcggtgaag aatttgaagc tgcgttcggg
     4081 tacgctgtcg gggttcaggc ggctgccgtc ttggtcgcgg ggtttgcttt ggctgtagcc
     4141 tgcctgtatc tgccattcgg gcgtgatgcg gccgccgact tcgatttccc agccgtgggt
     4201 tttggcttgg ttggcggcgc gatagtaggt gttgccgctc tggtcgcgtc ctgctgcggt
     4261 ggcgaggttg tttttacggg cgcggtacac ggcggcggat gcgttcagac gcccttcaag
     4321 ccattcgcct ttgatgtcgg cttccagatt gttgccggtt acgggtttca ggtagctgcc
     4381 gtgttcgtct ttttgcaatt gcgggacgaa caggctgctg tacgagccgt aaagcgacag
     4441 gttgccggtc agatcgaaca cgatgcctgt gtagggggtg aaacggttgg cggacacata
     4501 ggtcatgcct tgtgtgcggc tgttgtagct gcctgcgcgg tagcggctgt atctgccgcc
     4561 gagtatcagc gaaaggttgt cggcggcgcg gaaacgggtg gcgagatagc cgccgatttg
     4621 ccgcctggtg tcgtattgcg ggatggtttg ggcaaacgat gatggctgcg gataggcgcc
     4681 cgtgcgggaa aattcgtagg cgttgggaat ggcgttggga atgatgctgc gttcgccgta
     4741 tttgttgctg gcgtatttgt agccgttgat acccgcgatt aaatcgtgct cgcggccgaa
     4801 caggcggtat ttgccggtca atgacatgct ggcgctgtgg gtgcgcggat cggcgtgcca
     4861 ataaccggga atcaggtcgg tggcggcagt gctgtggtcg atggaaagta cgcctgccac
     4921 accgtagggc tggcggaagc ggctacgggt gtagtcgtat tcggctttga gtttccagtc
     4981 ttggttgaag cggtgttcta tgccggcgaa caggttgagc gcacggttgc ggctgttcga
     5041 ccaatttgtg gcggggttgt cttttgggcc gaaggcggtg gcataacctt ggctgtcgta
     5101 cacggcgtag ctgagcggcg cgtctgcggt ttctttcgcc tgctggtagt ccatgcctgc
     5161 gtggacgcgg gtttgcggtg cgatgtcgta ttccaaaatg ccgtagagtt cggcatcgcg
     5221 gctgcgttcg agctgccgcc acgagtcgcc gcgtccgaag gtggaaacca ggcggccgcg
     5281 cagcgtgcct tcggcgttca ggctgcccga tacgtccgcg cccagcccga aatgtttgcg
     5341 gttgccggct tcggcgcgga cttcaaacaa tggcttgcgg gtcgggtgtt tgcgtaccag
     5401 attgacggtg gcagaaggct cgcccgtgcc gtccggcagc cccgccacgc cgcgcacgac
     5461 ttccacgcgc tcataggcgg cggtgttggc attgcccgta tcggccagcg cgtcggcaac
     5521 ggggatgccg ttgatttggt agttggcgat gcggctgccg cgcgcgaaca ggtagttgta
     5581 gcccgcgcgg tcggagccgg gctggtgccg gtcgcctgca acagggcgcg gtcgagcgtt
     5641 ttgatgtttt ggtcgcgcat ttgttgcgat gtgatgacgc tgacgctctg cgggatttcg
     5701 cgcagggtca tgggcagccc gaacggggtg tgcgtgccgg aaacggtgta gccgtcgttg
     5761 gaactcgcgg tgcggtcggc ggtaacggtg atggtcggca attcggtgct ttcctgcggt
     5821 ttggggtcgt ctgaaacaga aacatcggcc tgcgcgtaca cgggtagcag ggcggcaaaa
     5881 agcagggagt atttgaagcg tgtcatgggg tttcctttgt gtgttggtgt gcggtggggt
     5941 tgtatagcgg attaacttta aaccggtacg gcgttgtccc gccccggctc aaagggaacg
     6001 attccctaag gcgcccaagc accgggcgaa ccggttccgt accatttgta ctgcctgcgg
     6061 cccgccgcct tgtcctgatt tttgttaatc ctctatgctt ttggcatatt gaaggtgtag
     6121 gctgctttta gcttcgcaga aactcagctt ccttcggaaa cttcgttttc aggcggcctc
     6181 ggacgggtcg ggcagatcaa tcagcaccac cgagcagccg ttttccgaac gcgggcggtg
     6241 cgacaccgac ttcggcacga ccgccatctc gccctcgcgt atcgtcatgt tgccgccgtc
     6301 ggcgaagtcc accgccatgt cgccctccac ggcaaacagc actttgtcgc tgtatccgtg
     6361 cgtgtgccag ccgtaatcgc ccaaaagttt gaccaggcgg aattggaacc cgtggcggtt
     6421 gatgatttcg ggctgccagt attctttgat ggcggcaagg tgctgtttca ggttgatggt
     6481 ttcgttttgc atggggttct ccttgtggtt tgttttgaat aaaactactg cgccgcaacg
     6541 ggttctgcct tttcaaacgc cgccttcaac tgttccgccg cctgtatcaa ctgccgcgcg
     6601 ccgcccgcga caatgtagtt cgcggcaggc atgacgatga tttgcttgcg cttccaagcg
     6661 ttcgtgccgc ataccagcgc gttatccaac acttccacgg cagccggccc ttcctgcccg
     6721 atggcggcgg tgcggtcgat gatgaaaatc cagccggggt ttttctcttt gatgtattcg
     6781 aaggaaacgg gctgcccgtg cccttcgttg cgtaaagatt cgtccacggg cggcaggccg
     6841 atgtcgccgt gtatccaact tgccaaccgc gattgcgtgc cgaaggcgga caccttgttg
     6901 cctgtaaccg acagcaccag cccgcgtcct ttgcctttgg cggcttcgcg cttttgggcg
     6961 aacagcgcgt caatctgcgc attcaattcc gccacgcgcg cttccttacc gaaaatccgc
     7021 gacagggtct ccatctgctt ctcgccgctg gtgcggatat tgccgttgtc caccgtcaaa
     7081 tctatggtgg tcgcgttttt cgccaactgt tcatacgctt ccgcacccgg cccgccggta
     7141 atgacaaact gcggattgtg gcggtgcagg gattcgcaat cgggctcaaa cagcgtcccc
     7201 accgttgccg ccttgtcaaa tgcaggctgc aaatagtcca cgcgcaccgg cgcggtggtt
     7261 gcgcccacat tcacgcccgg ctcggtcagc gtatccaacg ccgcccagtc gtacacggcg
     7321 acgcgttcgg gattcttcgg cacaacggca tcgccccgcg cggtcggcac ggtcagcgtg
     7381 gcgacaggtg tggatgcggc ttgggatgcg gcggatacag ttttttcggc ggcaggttcg
     7441 ggcgaacagg cggccggcag gacggcgcag gctgcccaat aaaaacgcgg tttcacggtg
     7501 tgcggttcct ttgcatacga ataagaatgt ttattattaa gcaaacaacc tgcagaaacc
     7561 acgccgcaaa acgcaaaatt cccgatacgg caaaacaaat tcccgatacg gcaaaacaaa
     7621 ttcccgatac ggcaaaacaa attcccgata cggcaaaaca aattcccgaa acggaaaagg
     7681 tcgtctgaaa caccctttca gacgaccttt tccgttccaa ccaatccctg cgcttgatta
     7741 ttggtaataa ttcctattta attcatttgt tagacaactc gttcctatcc aatcatgaac
     7801 accgccgcca tctaccgcca gtaccaaacc tatgtccgct ccgataaatc cggctgggcg
     7861 ttggacggct gttccgacag cgcactcatt gcgcaggcaa aacagcccgg tttgcatctg
     7921 aaaatgtgca tcaaccgctt cgactcgggc atcaccttat cacggatgcg cggcggcgga
     7981 acgggtgcgt ttcccaccga aatccacaat ttcagccaca actgcgcctt gttcgtcatg
     8041 gtatcggggc agaaccggtt acaaatgggc ggcagggaat accgcccgtc tgccggcgaa
     8101 atctggctgg tacgcggcga actggcggac gtatccgaaa ccctgctgcc agacagcggc
     8161 ggcatgtgtg cgctgcattt ggatttttcg ctggaaaaac tgcgccgctg gcacgacgaa
     8221 ggtttactgg acgaacgcct gttttcgccg caaacgatag gccgattcgc cctgcaacgg
     8281 ctggcgcaaa acgcggggac gctgacggcg gcggcctgcc ccctgctgca acgcccgttc
     8341 gaatcggacg gcttcggcct gctcgccgac gaagccgacg cgctggaact ctccgcccga
     8401 ttattacgct tcaccttccg ccgccacgac aacggctacc gccgccggcg catagacgaa
     8461 gccgccgata ttttgaacag cgaattcgcc cgcccgctga ccatcgccga aatcgcccgc
     8521 cgcgtcgggc tgaacgaatg ctacctgaaa cgctatttca aagcgcaaac cggcgaaacc
     8581 gtcgccggac gcctgcgccg cctgcggctg gaacacgcgc tcgccctcat cgaatcgggc
     8641 agcaccatcc aagccgcgat gcacttttgc ggctaccgcc acgcgggacg gttcaacgag
     8701 gcgttcaggc ggcattacgg atttttgcct tcggatgtga aaaagtgttg atgggccgac
     8761 ggcatagata ttttgatttg aaacaaccat taaaaaaggt cgtctgaaaa agcaatttca
     8821 gacgaccttt tttcattcgc ggcggcagtg tcgccccctg cccttatccc aaaaattctt
     8881 tcaaaaacag caacacgcag gcgagtttgt cggcggattc gcgttgggtg ccgttgccgg
     8941 tatggccgcc gccgtcaggc gagtagagcc aagattgcgg cgaggtttcg cgcagtttgg
     9001 cgtagaattt gagcgcgtgg gcgggatgga cgcggtcgtc gctgaggctg gtggtaatga
     9061 gtgcgggcgg ataatcgatg ccgtctgaaa gattgtgata cggcgacaat tcgcccagcc
     9121 ggcgtttgca ggcttcgtat ttctgcggat tgccgtattc gtccgtccaa cttgaaccgg
     9181 cggacagcag cggataacgg atcatatcgg tcagcggtac ttcgcacacc agcgcaccga
     9241 tgctttgcgg ttcgcgcacg aaggcggcgg cggtaatcag gccgccgttg ctgccgccct
     9301 gcaagccgat gtgtttgggc gaactcatgc cgcgttcgga caaatcacgc acgactgcca
     9361 acaaatcatc aacgcttttg tgtttgctga ttccctgcgc cgcctgatgc cagcgcgggc
     9421 cgaattctcc gccgccgcgg atgtttgcca atacaaaggc attgccctct tccagccaat
     9481 atttgccgac gctgcccaga taatgcggca attcaggaat gccgaaacct ccgtaagcat
     9541 agactaaggt cggcgtgtcg ggcgcggcgt ttttgccgac gtggaaataa ggaatgcgtt
     9601 cgccgtcgga cgacaccgcc caaaactgcc gcacttcgat gccgtctgaa acaaactgct
     9661 gcggctggag gcgcatgacg gtcagttcca tcacgttcaa atccagcgca aacagcgtca
     9721 gcggcgtggt gaaatcgctg gcggcaagat aaaccacgtc gccgccccac ggttggtcgg
     9781 tcatttccaa cgcgcccgag ggcaggtgcg gcaactcggc ttcctgccat ttgctgtcgg
     9841 caaaccgcca cgctttcaga cggccttgta cattctccag caggcttgcc accacaaaac
     9901 gcttggtcgt ttccacgctt tccaatgcct gcgtttcatc gggcgcaaac aaaagctgcg
     9961 ccgccccgag ttcgccccga ttcagtttca ccgccaccaa cgcgccactc ggatagcttt
    10021 ggttcgcgcg gtgccagtcc ttgcgcagcg tcagcaaaag atgtcccgcc agatagccga
    10081 ccacatcgca atcattaggc aggttcaacg gtttcgcccc gccttcggac gacacctgca
    10141 aatacgtctt ggtgtaaaaa ccgtccgacg cttcaatcaa atcaatcggc gaaccctgcg
    10201 gatcgaggta acgccacgcg tttaccatca tcgcgccttt atcgatttgg tacgccggca
    10261 ggctttcctc gaaactcttg ccgcgttcca ccagccacac ttcgcgcgga tagcccgatt
    10321 cggtcaactg gcgttcgtcc caagccggac acacccacac gctgttttca tcgcgccacg
    10381 acacatggtt tttgcctgcc ggaaagtgaa aaccgccctc taccaattcc cctgcttcca
    10441 aatccacttc cagcgtatac gccgtatcgc cgcccgattt gttcaaagtc agcagcgcgc
    10501 ggttgggctg ctccaccaag tgcgacacgc cgcccaaata cacatcgtcg ccgagcaact
    10561 catcgaaatc cgccaccgaa aacaggattt tccactcggg atagccggaa cggtaggtcg
    10621 ccgccgtaca cacgcggtac acgcccttcg gatattccgc attctgatgg aaatggtaca
    10681 tccgcgcgcg gtgttcctga caaaacggaa tctgccgcgt gtcctgcatt tgattcaaaa
    10741 tgccgtctga aagtgcgcgc gccttgtcgt tgtttaaaaa acgcgcgcgc gtttcggcat
    10801 tcgcttcagc agcgaagttt tgcgtttcgg cggaatcgag gttttcaaaa tggcggtagg
    10861 ggtcggggta ggatttcatc ggggtccttg agggtcgggc aggtctttat aatcggggaa
    10921 atgccgtctg aaacggggtt caggcggcat ttctgcggcg gctttccgtt gcggtcagcg
    10981 gtgcaggcga cgcaccagaa tatgcgggtt tcgtccgttg ctgcggtagt ctttgcgccg
    11041 cgtttcgggc agctcgtctt ccgatgccgt ctgaaagccg cgttcggcaa accattcgcc
    11101 ggtatttgtg gacagtgcga acagcctgct tatgcctatg ccgcgcgcct tatcgataat
    11161 gtgggcaagc aggcgttcgc cgtagccgcc gtcctgtgcc tgcggcgaga cggcaaggca
    11221 ggcgatttcg ccgcaatcgg cttcggcaaa ggttttgagt gcggcacagc cgtacaggtt
    11281 gccgtcgtgt tcgaggatgg aaaattcgga aatgtggttt tcgaggtatt cgcggctgcg
    11341 gtgcaatagg atgccctgtt cttccagcgg gcggatgagg gcggcgatgt gcgggatgtc
    11401 gccgctgtgc gcctgccgga tggagacgaa ggcttctttg gcaatggacg tgccgatgcc
    11461 gttgcgggtg aagagttctt gcagcaggct gccgtcggcg gccccgttga ggatttggac
    11521 gcgatgcacg ccgccttcga gcgcggcaac ggcggacgaa atcagtcgtc gggtttcgct
    11581 ggcggcgtgt tccgccagcg attgcgcttc ctgtgccgag agggtttcgg cgagcgtgcc
    11641 gtcggggcgg gaaatgccgt ctgaaagggt caggtaaacg agtttttcgg cctgaagcga
    11701 gacggcgacg gaagcggcgg cctgcaccat atcgagattg aaggttttgc cgccgtagga
    11761 atgcccgagc ggcggcatcc agacgatatt gcccgcgtcg agttggaaac ggagggcggc
    11821 ggtgtcggtt ttgcggataa cgcccgcgta ttccatatcg gttccgtcaa tcacgcctat
    11881 cggacgggcg gtcaggaagt tgcccgatac gagcgggacg gaaggcgcgc gcgcgaatcc
    11941 tgaaacgctg ccgcacaatg cggcttcaaa acggctgcgg acggtgccgg caaactgctg
    12001 cgcctgtccg agcgaggttt cgtcggtaac gcgcaaaccc cggcaataat gcggcgtgcg
    12061 gccttgcgcg gcggcgaggc ggtcgaggaa gtggtacgcg ccgtggatga ggacgagtcg
    12121 gatgcccagt tgcgacaaca gcccgatgtc ggcggcgagc ttatttaagg tgccgccttc
    12181 gagcaggcgg ccgtctatgc cggcgaccag tgtcgtgccg cgcatttggc ggatgtaggg
    12241 ggcggcttcg cggaagtggg cgacaaagct gtcgggcgcg ttcataggat gaagaggtag
    12301 gaaagctgca taatgaggac gataagtgcg aacagggttg caatagtcca gttgagccct
    12361 tcctgacggg cgggcggtac ggaaacctgc ggtgcggcgg caggtgcggc agcggcggtt
    12421 gcgggcggcg tatcgtgcag ggttgtaccg ccgttgagga tatcggcgat ttcgtcgcgg
    12481 gaaatctgtt tcttgctaat ggcgtgcgtg ccgatgcggt ggacgagttt gacatccgaa
    12541 acagcttcgg gcaaatcgtt gaatataggt tctttcgtgc ttgccagatg gtctttggct
    12601 ttaaacagcc cttcgcattt ttggcagacg acgaagcctt gggcgacgtt gagctgggtt
    12661 tctttgaccc agaggcgggt tttgcagtgc ggacagaaac aggcgggcat gatgttttct
    12721 cggtgtgtgt cggtttgatg ccgtctgaag cggcaggtgg ttcggacggc atatgcttta
    12781 ttctacgccg tactgccggc ggtaggtgcg gacgggttcg aggaactgtc cgaattcggg
    12841 gttgttttgc aacaggataa acaaatcgtt caggctggcg atgggggcga cgggcaggcc
    12901 gtattgtttt tccacttcct gaacggcgga caatttaccc gtgccttttt ccatgcggtc
    12961 gagcgcgatg gcgacaccgg cgggggttgc accctccgct tcaatcagtt tgattgattc
    13021 gcgtacggat gtgccggcgg aaatcacgtc gtcgataatc agcacgcgcc ctttaagcgg
    13081 cgcgccgacc aacacgccgc cttcgccgcg gtctttggct tctttgcggt tgtaggcaaa
    13141 cgggacgttc acgccttttt ccgccagcat catcgcggtt gccgccgcca aaataatgcc
    13201 tttgtaggcg gggccgaaca gcatatcgaa tcggatgccg ctttcaatga tggattgtgc
    13261 atagaatttt gccagttgca gcgtggacgc gccgtcgttg aagaggccgg cattgaagaa
    13321 atagggcgac cgccgtccgg ctttggtggt aaattcgccg aatttcaaaa cattttgggc
    13381 gagggagaat ttgaggaaat cttggcggaa atcagtcatt ttgtgctttc tgtcagatat
    13441 tgggacgcag ttgcgattct accgccccgt gcggcgcgct tcaactgccg ccgccttgcc
    13501 gcaaggcgcg tttcaactgt ttcaacacct gccagtttcg ggctttgagt tcgggttggc
    13561 gcaacagccg ggcgggatgg tcgatgatga agaagggacg gctgccgcac aaagtttcaa
    13621 tcatcgcctg ccgttccagg ttgacaaacg cctgtccgag gaataggacg gcgggggcgc
    13681 ggcagccgtc gagttccccg gcgatttgac ccagcgcatc cgcgacggcc tgttcagacg
    13741 gcatcgggtt accgacggcg gcggttttca cccaacaggt tttgtggaca taggcggcat
    13801 ccagtcctgc ggctttgagg atgttgtcga gcaggatgcc cgctttgccg tggaacagtt
    13861 gcccgtaaac cgcatcctcg atcggcggac acaggctgac gacggcaagc ttggtaatgc
    13921 ccgaagcggc gggaacgggg gcgatgccgt ctgaaaggcc gggcgggggc gtttcggttt
    13981 caggcgcggg tttgcgcgta tgtacggcgg cggtttccaa cgctttcatc gtttcgagcc
    14041 gcgcctgaac gttatggggt tgggaagggc ggatcgtggc ggcgcggacg gtttggggac
    14101 gtgcctgtgc cggggttgcg ggtgtgtttt tgggcggcag gacggcggcg gcctgtttca
    14161 gccacatcgg acccaagccc aaagcctcgt gcaggtggag gtagcgcgcg cttaacatat
    14221 tttctccatt aagacggcat cttcggtttg cccgtcggcg gcgcagtaat agtttttccg
    14281 cctgcccgca atgctgaagc cgtgtttggt atacagtgcc tgtgcggcgg cgttgcctgc
    14341 gcggacttcg agcagcaggc gttgcgtgcc ttcgggcaga tgtgcgtacc aatattcgag
    14401 cagggcggac gcaacgcccc gtcggcggca ttcgggcgcg gtggcaatca ggtgcagttc
    14461 ggattcgtcg ggcaggttct gccaaacgat aaaggcggca atcctgccgt ctttttccgc
    14521 aaggaaaacc tgttcggacg gcgaaacaag cgcggactca aattggcgtt gcgtccacgc
    14581 ggacgggttg cagacggtat cgagcgcggc cagtgcggcg cagtcggacg gtgaggctgg
    14641 gcggatgttc atgttcgtgc cttccgttcc gcctgttctt tggcagtcag ggcgattttg
    14701 ttgcggacgt agagcagttc ggcgtgtgcc gcgccagttg cgggatagcc gccgagggcg
    14761 agcgcgagaa aatcggcggc ggtcggcata tcgggtttgc ctgagaaggg cggacggttt
    14821 tccagtgcga acgcactgcc gatgccgtct gaaaagacgt acccctcggg gagggcaatg
    14881 tctgccgccc taccgacttg ataatcgctc aaacggcggc ggttcagcgt gtcgaaccac
    14941 gcataaaaca cttcgcccat acgcgcgtcc gcagcggcga gtatgcagct ttgcggcggc
    15001 ggcagcgagg cggcggcatc gagcgtgggg atgccgatta aaggcgtgtc gaacggcgtt
    15061 gccaaacctt gcgccacgcc gatgccgata cgcagtccgg taaacgcgcc ggggcctttc
    15121 gcataaacaa tcgcccccaa atcggcggcg gtaatgcccg catttcggaa tagggtgcgg
    15181 atttccggca ggatcagttc ggactggcgg atgccgactt cctgatggaa cagacggatt
    15241 tcgccgtcgg cgcgcagcgc gagcgacaaa taggaagttc cggtatcgac ggcgaggacg
    15301 gggcggttga aatcggcttg catggtgtgg ttctcgttgg ttcagacggc attatatagt
    15361 gaaatcggct tgcctgccgt gccgtcgtgt cctagggcgg tatggcgcaa aaatgccgtc
    15421 cgaacggtaa attatcgtgt tcggacggca tttttcaaat actactgttt gtcggcgatg
    15481 ccgatttcgt gaaccttttc ccctatcttc acggttgccg agccagcgat ttcttgggcg
    15541 cggtcgccga aaagggcgag gcggtaagtg cctttctctt cgccgccgta gcgcgtgtcg
    15601 cccaaaatga cggcgtgtga tttttcatct gctttgagtt cggcggaggc aagctcaaca
    15661 ttctgctcgg gtgttttcag gtgttcgatt ttgccgtgtc cctgtttggc ggcgaaatct
    15721 atggtatagg tcagttttcc gccggcatcg tcggagctga atgctttgcc gtgatactcg
    15781 gctttgccgt caggcagttg gttgaaggcg gtatgttctc cgcccaaatc gctgacaagg
    15841 aaggagcgtt ggtttatcag gctgtcgatt ttgtcggggt tgttgatttt ttcaatccgt
    15901 agggcaacga cggcggagtg atcctgtttg tatatttgaa attcgccgct tgccagtgtg
    15961 atggtttgtc cgtccacttc gattttttgc acgaagtcga agcggctgat tttgtcgttc
    16021 ttcagtttgc ccgtgttgag gctgttgtct ttgccgccgg ctttgaaagt tttttccgca
    16081 ccttgtgccg acagggtcag tgttccgttt tggggaatgg aggcttccaa tgttagggat
    16141 ttcaaacctt tgtctttatg gtcgagcggc gcggttaatg catcggcaag ccccgtgccg
    16201 atgtcggcgg cgacaccacc gcctccgccc ctccgctgct gcaggcggtc agaatcaggg
    16261 ccggcggtca aagaaaggca gcagaaggta gttcggttca caggtttact cctagtcata
    16321 cacagaatag ataatatata aacgttttgg ttatggtatc tttttttgca tactgcatca
    16381 atgaggcagg tcagagaagc aaaaatcaaa tgccgtccga acggcgttca gacggcattt
    16441 tgtttacagg caacctgtta tttgacgatt tggttcaatt cgcccttggc ataacggctt
    16501 gccatttttt ccaacgaaac aggtttgatt ttgcctgcct gaccttcgca accgaacgca
    16561 agataacggt cgaggcagat ttgcttcatc gcttcaatgg ttttgcccaa gtatttgcgc
    16621 ggatcaaagt cggacgggtt ttcggcaagg tagcggcgta ccgcgccggt ggaagcgagg
    16681 cgcaggtcgg tatcgatgtt gactttgcgc acgccgtgtt tgatgccttc gacgatttct
    16741 tcaaccggca cgccgtaggt ttcgccgata ttgccgccgt attcgttgat gactttcagc
    16801 cattcttgcg gaacggagct ggagccgtgc atcacgatgt gtgtattggg cagggcttgg
    16861 tggatttcct tgatgcggtc gatacgcaat acgtcgcctg tgggcggacg ggtgaatttg
    16921 tatgcgccgt ggctggtgcc gacggcaatc gccaatgcgt caacgccggt atctttaacg
    16981 aaacgcacgg catcttcaac gctggtgagc atttggtcgt gtgagagttt gcctgccgcg
    17041 cccactccgt cttcttcgcc tgcttcgccg gtttcgaggt tgcccaatac gccgatttcg
    17101 ccttcgacgg acacgccgca ggcgtgggag aagttgacga cggtacgggt ggcgttgacg
    17161 ttgtattcgt aagaagaagg ggttttgccg tcttcgagca aagagccgtc catcatcacg
    17221 gaggagaagc ccagttggat ggagcgttgg cacacgtcgg gcgatgcgcc gtggtcttgg
    17281 tgcatcacga cggggatgtg cggaaattct tcgactgccg ccagaatcag gtggcgcaaa
    17341 aacggcgcgc ccgcgtattt gcgcgcacct gcgctcgcct gtacgatgac gggcgcgttg
    17401 acttggtcgg cggcttccat aatggcgcgc atttgttcga ggttgttgac gttgaacgcg
    17461 ggcaggccgt agctgttttc ggcggcgtgg tcaagcagtt ggcgcatgga tacgagtgcc
    17521 atttgtgtct ccttgggcaa taggtaaata aggcggatta taatgttttt tacggcaaaa
    17581 aaccataaac ggctcattga ttttatatta acgataacgg cgggcagatg gggcggattt
    17641 tgggcggttc gggattttgg tgtgttgttt atgataatgt tttcactggt tttttgatat
    17701 ttgtgtggga cggttatggt tttggacggg tttgcggcgt attttgacgc ttatttggaa
    17761 aacatcgtgc gcgagggcaa gtcggagcac acggttgcgg cataccggcg cgatttggaa
    17821 gaactgtttg cactgttggc acaaatgccg tctgaagatg caggcggcgt gccgcaggac
    17881 ttgtcgcggc gcgattttac ggcggcgttg cggcggctgt cgcagcgcgg tttggacggt
    17941 cggacgctgg cgcgcaagct gtcggcgtgg cggcagtatt gcgcctggct ggtcaaacgc
    18001 gggctgatgc gcgccgaccc gaccgccgac atcaaaccgc cgaagcagcc cgagcgcgta
    18061 cccaaagccc tgccgcagga atggctgaac cggatgttgg atttgcccgt ggacggcggc
    18121 gacccgctgg cggtgcgcga ccacgcgctg ttcgagctga tgtacggcag cggtttgcgc
    18181 gtgagcgaga tacacggctt gaatgcagat gatgtatatt tggacgaagc gtgggtacac
    18241 gttaccggca aagggcgcaa gcagcgtcag gttccgctga ccggcaaaag cgtggaagcc
    18301 ttgaaaaact atctgccgct gcgtcagacg gcatcggacg gcaaagccct gtttaccggc
    18361 aggaacggca cgcgcctgag ccaacgccaa atccaaaaac gcctcgaatc gtgggcggcg
    18421 caatacggcg acggcaggca cgtttcgccg catatgatgc gccacagcta cgccggccac
    18481 ctgttgcagg cttcgcgcga catcagggcg gtgcaggagc tgctcggaca cagcagcctt
    18541 tcgaccacgc agatttatac caagctcgat ttcgaccaca tcgcccgcct ctatgacgaa
    18601 gcccacccgc gcgccaagcg gcaggacgaa tgacatacgg caaaatcagc cgtcagccgc
    18661 acgctcttga tatataattg accgttgcac ccggacgaca cataaaaaag acacaccatg
    18721 aacccaagcc ccctactcga cctgattgac agcccgcaag atttgcgccg cctggacaaa
    18781 aaacagctgc cgcgccttgc cggcgagttg cgcgcctttc tgctggaatc tgtcgggcag
    18841 accggcgggc atttcgccag caatctgggt gcggtcgaac tgaccatcgc cctgcactat
    18901 gtgtacgaca cgcccgaaga caagctggtg tgggatgtcg gacaccaaag ctacccgcac
    18961 aaaatcctga caggcaggaa aaaccagatg cacaccatgc gccaatacgg cggtttggcg
    19021 ggttttccga aacgttgcga gtccgagtac gacgcgttcg gcgtggggca ttcctccacc
    19081 tccatcggcg cggctttggg catggcggcg gcggacaaac tcttgggcgg cgaccgccgc
    19141 agcgtcgcca tcatcggcga cggcgcgatg acggcggggc aggcgtttga agccttgaat
    19201 tgcgcgggcg atatggatgt ggatttgctg gtcgtcctca acgacaacga aatgtcgatt
    19261 tcccccaacg tcggcgcgtt gcccaaatat cttgccagca acgtcgtgcg cgatatgcac
    19321 ggactgttga gtaccgtcaa agcgcaaacg ggcaaggtat tagacaaaat acccggcgcg
    19381 atggagtttg cccaaaaagt cgaacacaaa atcaaaaccc ttgccgaaga agccgaacac
    19441 gccaaacagt cgctgtcgct gtttgaaaat ttcggcttcc gctacaccgg ccccgtggac
    19501 ggacacaacg tcgagaatct ggtggacgta ttgaaagact tgcgcagccg caaaggccct
    19561 cagttgctgc acgtcatcac caaaaagggc aacggctaca aactcgccga aaacgacccc
    19621 gtcaaatacc acgccgtcgc caacctgcct aaagaaggcg gggcgcaaat gccgtctgaa
    19681 aaagaaccca agcccgccgc caaaccgacc tatacccaag tattcggcaa atggctgtgc
    19741 gaccgggcgg cggcagattc ccgactggtt gcgattaccc ccgccatgcg cgagggcagc
    19801 ggactggtgg agtttgaaca acgattcccc gaccgctatt tcgatgtcgg catcgccgag
    19861 cagcacgccg ttacctttgc cggcggtttg gcgtgcgaag gcatgaagcc cgtcgtggcg
    19921 atttattcca cctttttaca acgcgcctac gaccaactgg tgcacgacat cgccctgcaa
    19981 aacctgcccg ttttgtttgc cgtcgaccgt gcgggcatcg tcggcgcgga cggtccgacc
    20041 catgccggct tgtacgattt gagcttcttg cgctgtgtgc cgaacatgat tgttgccgcg
    20101 ccgagcgatg aaaacgaatg ccgcctgctg ctttcgacct gctatcaggc ggatgcgccc
    20161 gccgccgtcc gctatccgcg cggcacgggt acgggcgcgc cggtttcaga cggcatggaa
    20221 accgtggaaa tcggcaaggg cattatccgc cgcgaaggtg agaaaaccgc cttcattgcc
    20281 ttcggcagta tggtcgcccc cgcattggcg gttgccgaaa aactgaacgc caccgtcgcc
    20341 gatatgcgct tcgtcaaacc gatagacgaa gagttgattg tccgccttgc ccgaagccac
    20401 gaccgcatcg ttacccttga agaaaacgcc gaacagggcg gcgcaggcgg cgcggtcttg
    20461 gaagtgttgg cgaaacacgg catctgcaaa cccgttttgc ttttgggcgt tgccgatacc
    20521 gtaaccgaac acggcgatcc gaaaaaactt ttggacgatt tgggtttgag tgccgaagcg
    20581 gtggaacgcc gggtgcgcga gtggctgccg gaccgtgatg cggcaaatta aaccgcttga
    20641 ccgcgccgtc gttatcgggc ggcgttttta aacggcgttt gtttctgcgg tttttttatt
    20701 gaaaccccgc aggcggcagg aagggttcgg gcggcggctt tcgggcggtg cttggtgtgc
    20761 cgttgcgcgt ttggaaattt attccgcttg tccgtataac ggcggcggtg ccgtctgccg
    20821 atacaaggca aaatgccgtc tgaaacgctt caggcggcat ttttcggcgt gagggtttta
    20881 ggcttcgaca attttgccgc gcagggaaaa ggtgtaggct tcggtgattt ccaaatcgat
    20941 catttggttg atcatgtcgg gcgtgccggt aaagttgacg acgcggttgt tggcggtacg
    21001 ggcttggagc tggtcggggt cttttttgga gatgccttcg accaggcagc gttgaaccgt
    21061 gccgatcatg gtttggttga tgcgggcggt ttcggcttcg atgacttcgt tcaaggcttc
    21121 gaggcggcgc actttttctt cgtgcggcgt gtcgtccggc aggttggcgg caggcgtgcc
    21181 ggggcgcggg ctgtaaataa acacgaagct caagtcgaag gcaatgtctt tcaccagttt
    21241 caaggtttgc tcgaactcgc gttcggtctc gccggggaaa ccgacgatga agtcgctgct
    21301 caggcacaaa tcaggacgga tggcgcgcag tttgcggatg atggatttgt attccaaagc
    21361 ggtgtagccg cgtttcatcg cgctcaatac gcggtcggaa ccgctttgaa tcggcaggtg
    21421 caggtgggaa accagtttgg gcaggtcgcg gtagcactcg ataatcgagt cggtaaactc
    21481 gcgcgggtgg ctggtggtga agcgcatacg ttcgatgccg gggatttcgt ggacgatacg
    21541 cagcagggtg gcgaagtcgc agatttcgcc gtcgtccatt tcgccgcgat aggcattgac
    21601 gttttgtccc aagaggttga tttctttcac gccttgctgg gcaaggttgg cgatttcggt
    21661 caatacgtcg ttgagcgggc gggagaattc ttcgccgcgc gtgtagggga cgacgcagaa
    21721 ggagcagtat ttggaacagc cttccataat cgacacaaat gccgcgccgc cttcgacgcg
    21781 ggcgggcggc aggtggtcga atttttcgat ttcggggaag gaaatatcga cttgcgacag
    21841 cccgctggtt tctttgtcca caatcatttt gggcaggcgg tgcagcgttt gcggaccgaa
    21901 aaccacgtca acataaggcg cgcgtttgat gatgttttcg ccttcttgcg aggcgacgca
    21961 gccggcaacg ccgatgatga ggccggggtt tttttctttg agcggacgga cgcgccccaa
    22021 gtcggagaac actttttcct gtgctttttc gcgcacggaa caggtgttga acaagatgat
    22081 gtcggcttcg tcggcttggg taacctgttc gatgccgccg tgttcttcgg caaggacgga
    22141 cagcattttt tcgctgtcgt actcgttcat ctggcagccg aaggtgcgga taaatacttt
    22201 tttcatggtt tgtgtctttc tcaggcagcc gtaatcgcgg ggctgatggt tgttggaatg
    22261 aaaaaatttc agacggcacg acgatgccgt ctgaaaatcg gtgcggatta tagcacgatg
    22321 tgggtttggg aggcaaaata ttgttttaaa atatgaattt aatcggtcgg aacggctgta
    22381 taatgtttgg ctttaatggg aggtgtgtat gaaaccggtt gtatgggcgg cattgctgct
    22441 gtgtgcgtgt accagcaatt tcggcgacag ggaacatcag ttcctgcgtt atagtggatt
    22501 aaatttaaac cagtacagcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
    22561 cgtcgccttg tcctgatttt tgttaatcca ctatatcaga cggaagaggg aatcgcacaa
    22621 acggttgtta aaggcaaaac gacccgggcg gaggtagagg cacgtttcgg gaagcgcaac
    22681 cctttcggct gttatgccta tcacgaggtc agcctgccga tttataattt tttgccgacc
    22741 aatttcatct atatgaaatc ggagcggcgg cattgggaat ggtgcgcgga ttacgacggg
    22801 gagggagtcg tccgggacta ccgctttaca cataaagagg aaaaagacga gcgttccgtc
    22861 atccgggata cggttggcgt aatccgcaaa gaagcgggca aatccttgtc gcaacctgaa
    22921 aaatgataaa atggggcttt ctgcttccaa gcccgaaacc tgccgttcag acggcatttg
    22981 aggataaata tgaaccgtaa cgaaatttta ttcgaccgcg ccaaagccat catccccggc
    23041 ggcgtgaatt cgcccgtgcg cgcattcggc agcgtcggcg gcgtgccgcg cttcatcaaa
    23101 aaagccgaag gcgcgtatgt ttgggacgaa aacggcacgc gctacaccga ttatgtcggc
    23161 tcttgggggc ctgcgattgt cggacacgcg catcccgaag tcgtcgaagc cgtgcgcgaa
    23221 gctgcgttgg gcggtttgtc gttcggcgcg cccaccgaag gcgaaatcgc cattgccgaa
    23281 caaattgccg aaattatgcc gtctgtcgaa cggctgcgcc tcgtcagctc cggcacggaa
    23341 gcgacgatga ctgccatccg tctggcacgc ggttttaccg gccgcgacaa aatcatcaaa
    23401 tttgaaggct gctaccacgg ccattccgac agcctgttgg tgaaagcagg cagcggtctg
    23461 cttaccttcg gcaatccttc ttccgccggt gtgcctgccg actttaccaa acatactttg
    23521 gtactcgaat acaacaacat cgcccaactc gaagaagcct ttgcccaaag cggcgacgaa
    23581 atcgcctgcg tgattgtcga acccttcgtc ggcaatatga acctcgtccg cccgaccgaa
    23641 gcctttgtca aagccttgcg cggattgacc gaaaaacacg gcgcggtgtt gatttacgac
    23701 gaagtgatga ccggtttccg cgtcgcgctc ggcggcgcgc agtcgctgca cggcatcacg
    23761 cccgacctga ccacgatggg caaagtcatc ggcggcggta tgccgcttgc cgcgttcggc
    23821 ggacgcaaag acatcatgga atgtatttcc ccgttgggcg gcgtgtatca ggcaggtaca
    23881 ttatcaggca acccgattgc cgtcgccgcc ggcttgaaaa cgctggaaat catccagcgc
    23941 gaaggcttct atgaaaacct gaccgccttg acacaacgcc ttgccaacgg tattgccgcc
    24001 gccaaagcgc acggtatcga gtttgccgcc gacagcgtgg gcggtatgtt cggtctgtat
    24061 ttcgccgcac acgtgccgcg aaactatgcc gatatggcgc gctccaatat cgacgctttc
    24121 aaacgcttct tccacggcat gctcgaccgc ggcattgcct tcggcccgtc cgcttatgaa
    24181 gcaggtttcg tttccgccgc gcatacgccc gagctgattg acgaaacggt tgcggttgcg
    24241 gttgaagtgt tcaaggcgat ggctgcatga tgttttgacg gacagagttt ctctgttcga
    24301 tttgtttggc agattgaagt aagaatgcac accgccgtca tttccgcgca ggcgggaatc
    24361 cggacctttc agtttctgta atgattgaaa ataacggcaa gcccgacctt ccggattccc
    24421 gcctgcgcgg gaatgacggg cgtgtacatt tttgatttca atctactgta aaaatgccgt
    24481 ctgaaat
//
LOCUS       DAXFWM010000024        23928 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000024 DAXFWM010000000
VERSION     DAXFWM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23928
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.024"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..181)
                     /locus_tag="ACFI8B_000957"
     CDS             complement(<1..181)
                     /locus_tag="ACFI8B_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834212.1"
                     /translation="MPSERLSDGIFPCILNRVYETPDAFGGNVGCCAMFGGDQRTKCA
                     GKGLMPSEGSDGIGIG"
     gene            complement(199..756)
                     /locus_tag="ACFI8B_000958"
     CDS             complement(199..756)
                     /locus_tag="ACFI8B_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3834213.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(831..2465)
                     /locus_tag="ACFI8B_000959"
     CDS             complement(831..2465)
                     /locus_tag="ACFI8B_000959"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC3834214.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2528..2866)
                     /locus_tag="ACFI8B_000960"
     CDS             complement(2528..2866)
                     /locus_tag="ACFI8B_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834215.1"
                     /translation="MIFKCRPNAFQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVML
                     FVPDCPVGGAAGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLVF
                     LPCFAPLPYN"
     gene            complement(2863..3969)
                     /gene="serC"
                     /locus_tag="ACFI8B_000961"
     CDS             complement(2863..3969)
                     /gene="serC"
                     /locus_tag="ACFI8B_000961"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC3834216.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKIPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4335..4766
                     /gene="rimP"
                     /locus_tag="ACFI8B_000962"
     CDS             4335..4766
                     /gene="rimP"
                     /locus_tag="ACFI8B_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC3834217.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4794..6296
                     /gene="nusA"
                     /locus_tag="ACFI8B_000963"
     CDS             4794..6296
                     /gene="nusA"
                     /locus_tag="ACFI8B_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC3834218.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6308..9139
                     /gene="infB"
                     /locus_tag="ACFI8B_000964"
     CDS             6308..9139
                     /gene="infB"
                     /locus_tag="ACFI8B_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC3834219.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVGGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9299..10639)
                     /locus_tag="ACFI8B_000965"
     CDS             complement(9299..10639)
                     /locus_tag="ACFI8B_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC3834220.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10713..12053)
                     /locus_tag="ACFI8B_000966"
     CDS             complement(10713..12053)
                     /locus_tag="ACFI8B_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC3834221.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12196..12822
                     /locus_tag="ACFI8B_000967"
     CDS             12196..12822
                     /locus_tag="ACFI8B_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC3834222.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12887..14305)
                     /locus_tag="ACFI8B_000968"
     CDS             complement(12887..14305)
                     /locus_tag="ACFI8B_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3834223.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     MRKKAGQDLSYQYEFHEFHDKQ"
     gene            14632..15360
                     /locus_tag="ACFI8B_000969"
     CDS             14632..15360
                     /locus_tag="ACFI8B_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC3834224.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15419..15907)
                     /locus_tag="ACFI8B_000970"
     CDS             complement(15419..15907)
                     /locus_tag="ACFI8B_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC3834225.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGAAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16929..17393)
                     /locus_tag="ACFI8B_000971"
     CDS             complement(16929..17393)
                     /locus_tag="ACFI8B_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC3834226.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            17615..18673
                     /gene="alr"
                     /locus_tag="ACFI8B_000972"
     CDS             17615..18673
                     /gene="alr"
                     /locus_tag="ACFI8B_000972"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC3834227.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(19215..20570)
                     /locus_tag="ACFI8B_000973"
     CDS             complement(19215..20570)
                     /locus_tag="ACFI8B_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC3834228.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(20585..20857)
                     /locus_tag="ACFI8B_000974"
     CDS             complement(20585..20857)
                     /locus_tag="ACFI8B_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC3834229.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(20982..21719)
                     /locus_tag="ACFI8B_000975"
     CDS             complement(20982..21719)
                     /locus_tag="ACFI8B_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC3834230.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(21857..22756)
                     /gene="prmB"
                     /locus_tag="ACFI8B_000976"
     CDS             complement(21857..22756)
                     /gene="prmB"
                     /locus_tag="ACFI8B_000976"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC3834231.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            complement(22855..23052)
                     /locus_tag="ACFI8B_000977"
     CDS             complement(22855..23052)
                     /locus_tag="ACFI8B_000977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834232.1"
                     /translation="MGERLVQTDYAHPAVGIADIGHLVVFCGYPLLALNKPRFFPVYQ
                     VAHTVRHRRFRSGNGLRAAFM"
     gene            23088..23573
                     /locus_tag="ACFI8B_000978"
     CDS             23088..23573
                     /locus_tag="ACFI8B_000978"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834233.1"
                     /translation="MRGCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELGEFAET
                     KELRKAAMQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVALDKVL
                     MNAKYEGSFPNKLGRDHIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDHFILKD
                     K"
     gene            23713..>23928
                     /locus_tag="ACFI8B_000979"
     CDS             23713..>23928
                     /locus_tag="ACFI8B_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3834234.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVTNHHLALAADEVFEGPAGPGGS"
BASE COUNT         6319 a         6518 c         5896 g         5195 t
ORIGIN      
        1 ccccgatacc gatgccgtct gaaccttcag acggcatgag gcctttgcct gcgcacttgg
       61 tgcgctggtc gcctccgaac atggcgcaac acccgacatt tccgccgaac gcatcgggcg
      121 tttcataaac ccggtttaaa atgcatggaa aaatgccgtc tgaaagcctt tcagacggca
      181 ttttgtttga gattccgttt accaatggct gacaaacgct tccaaatcgg tattcttggg
      241 cttatgcact tcctctgtcg gcgtgccgac catcatcagc ccgatgattt tatccttatc
      301 ctcgcaaccg aaagcctccc gcaacaatgg gctgttgacc cacatccccg taatccagac
      361 attgtcgaat ccctgagccg ttgccgccag ttgcagcgca tacgccgcac aacccgccgt
      421 cagcatctgc tcccattccg gtttcggctt aggcacatcg cggttcggcg caaacgttac
      481 cccgatgacc atcggcgcca tattgcccac tttttccgcc tttttcatcg catcgtcgcc
      541 gaaattcagt tcggcaaccg tttgcttcaa cacatcgcga aaacgttgca atcctacctc
      601 gccttgaatc acggtaaaac ggaaggggcg catgttgccg tgatcggaaa cttgggttgc
      661 cgcctgcaat atttgttcca actccgccgc atcgggggcg gggtgcttca gctttttgga
      721 agatcggcgg ttcgtcaata attttaaagc atccatatcg ttatactccg gtcatcggtc
      781 gggtgtttcg gacacatatg ccgtgaaggc ttcagacggc atatccggca tcagcgcgga
      841 cggcggcagg ctgccaatat atccatttcc ttccgatagg tttggctgtt ggaaatgtcc
      901 atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat attcgttttc cgccgctttt
      961 tgtttgaggc attggaaatc tatgcccccg tgttggcgga aggctttgga aaaccacata
     1021 accatcggga tatgcgtctg tccggaaggc gcgatggcgt aaggcgcggc gtgcaggtac
     1081 atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat aatacaccgc gctttccaaa
     1141 tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa actgatccac atacaaaacc
     1201 gtattgtcgt aagtgttgac caacgtggcg cgggcgcatt tgtcgatttc gttggtatcg
     1261 catgtcggcg tgaatttgcg ctcggcttcg gtatagcgtt cgtaatacgt cggcccgtgg
     1321 ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt ttttgttgag ggcttcgtcg
     1381 aacttggtca acaggatatt gtcgaggcac tcgccgttgc ggcagtattc gggcaggttg
     1441 agcgaggtaa cgtcggtatt cggcactttg ccgcacacgc ccttgcagcc ggaatcgttt
     1501 tccaaccaag taacttccac gccggcgcgc tgtacgatgt ccagcaggtt gtcttggtgt
     1561 tcggctttga tttcgtcata atccgtgcgg tcgaaggttg agaacataca cggcagggag
     1621 tgcgcggtcg atgtgccgca gcttctgacc tgcgggaaat tgacaatttc atcgccgcgc
     1681 gcggcaagca gcggcgtagt ttggcggctg taaccgttca aaccccagtt ggcggcacgc
     1741 gtagtctcgc ccacgaccag caccacgaaa cggcgcaggc tgccggccgg ccggttttgc
     1801 acgaccgcca tatccaattg cgtataagga atattggaac gcttccaatc tttgtatttc
     1861 gacacgcccg cgccgatgaa attagacggc acaatcagat gggttactga tttattgttg
     1921 cggaaaaacg aggcgtaatc ctgatattgc aacatcgcga tgcccaacgc gcacaaaaag
     1981 gaaacggcgg caagcacaag gcgcgtcaaa agctccttat accaaacgcg gtatttgacc
     2041 ttgacggcga tatatgccag cgcgggcaat acgcccaaac atacaatcca cagcacatag
     2101 cccggcgtaa tcaggcgcgc gctttccgcc gccgtggttt gcaagacatt attcaacatc
     2161 gacttgttga aatagatatt gaaaaatatt tcttggtaag acaccgccgc actgataacc
     2221 aatatcaatg gaatcaatac tttatgcaca aaaggcaggg caatgacgtg aaaaacgaaa
     2281 ttacttaaaa aaaacagcac caccggcatc gtatagagga agatgtccgc cccggtgtcg
     2341 ttaaaagggc gaagttcgac aactttggca aaaaaggcgt aattcaatac cagcgaagaa
     2401 tacagggaaa gaaaggcaat cagcgcggaa gagccgagct tcggcctcag gttcggtttt
     2461 atcatttgga atgtgtcgga taagggttgg aaaaggcatc cggaatccgg attattgaaa
     2521 aagattctta attataaggc aacggagcaa agcagggcaa gaaaacgagt aggcggcgcg
     2581 ggggtttccg cccgccattc aaacgtccgg cagacataaa aacatcgtaa gcaaaattcg
     2641 aaccggtctg caaccgcccc tgccggaaaa gcgggcaaag catttcatat tggaaaaacc
     2701 cggccgcgcc gccgacggga cagtccggca caaacagcat cacgcccgga ttgaaaagga
     2761 cggatagccg ccggcagcac aatcttacca cctccaaaac gccgccggag aacgccgaaa
     2821 cagccgatgc cgtctgaaac gcattcgggc ggcatttgaa aatcaaccgt aacgccgttg
     2881 gaaatcgcgc ataaaatctg ccaaagcccg cacgccttca agcggcatcg cattataaat
     2941 gctggcgcgc atcccgccga cagacttata gcccttcaac aggcacaagc cctgcaactc
     3001 ggcttccaac acaaaacggc ggtcaagctc ctcatccccc gtttggaaca cgacattcat
     3061 tttagaacgc gcattcggac ggatacggtt gatatagaaa ccgccgctgc catctatcgt
     3121 ctcatacaag gtttgcgcct tcagccgatt gaccgcttca atttttttca caccgccctg
     3181 cgcctgcagc cagcggaaca ccagccccga catataaatc gcgtaagttg acggcgtgtt
     3241 gtacataccg tcgcggttca aatgagaacg gtagttgaac acatcgggaa tatcgttcgg
     3301 acaacgctcg agcaaatcct cacgcacaat caccaccgta actcctgccg gcccgatgtt
     3361 tttctgtgcg cctgcgtaaa tcagtccgta gtcggcaaca tcaaactcac gcgacagaat
     3421 ctcgctggac atatcgcaca ccaacggcgg catgccgtct gaaaggcgcg gcacttcacg
     3481 gtattgcagc ccgttgacag tttcattgac ggcaaaatgg acaaacgccg aatcgggtgc
     3541 aacatcccac gtttccacag gcggcaggtc gagatagtcg aactgctcgc ccccgtgcgc
     3601 cgccaaacgg atttccgtat cggtcaaacg gctcatctgt tcataggcga tacggctcca
     3661 gttacccgtt accaccgcgt cggcagtgcg gaaaccgtgt gccagattca tggctgccat
     3721 attaaattgg gttgttgctc cgccctgaag aaacaatatc ttatagttgt cagggatttt
     3781 caaaagctgc ctcaaatcct gttccgcatg atgcaggatg cttaaaaaca tttccgaacg
     3841 gtggctcatc gccatcacag gaaaacccgt accgttgtag tccgacattt cctgctgcgc
     3901 cgtcctcaac acagcttcag gcaacacggc agggccggcg gaaaaattgt aaatcggata
     3961 aagagacatg atgcagccct ggttctgaac aataacccgc ccgattttag gcttgcagca
     4021 ggctttgtgc aaggatggga aatacctgcc ctccgcccga ttccatgccg cccgaacacg
     4081 gaaaataata tcaatatatt gatttacaaa cataaaaatc atgctagcga caaatagata
     4141 catttgtttt gtcaacaata ttcacgattt cccattacaa acctcccgta cacccgcttt
     4201 tttccgtccc aaaaacacaa aataaatcaa cactttcatt tctccgcaaa agcggttata
     4261 atcacgccga tttttcaact tttgacgaaa aatggccacg gggccatttt tttattatgt
     4321 acatagggag cagcatggat attcaaacca tcctcgaaaa aaccctgccc ggcctgggat
     4381 acgaactggt cgatttcgaa ctggccgcgc aaggaacatt gcgcgtgttc atcgacaaag
     4441 aaggcggcat taccgtcgaa gactgcgcca ccgtcagcaa ccacttgagc cgcgtcttca
     4501 tggttgaaga catcggctac aaaaacctgg aaatttccag ccccggactc gaccgcccct
     4561 tgaaaaaagc cgccgacttc gtgcgctttg ccggtcagaa tgccaaaatc aaaacccgcc
     4621 tgccgatagg cggtcagaaa aactttatcg gtaaaatcga aggctgcgaa aacgacactg
     4681 ttaccgtttc cttcgacggc aaaaccgtgc aaatcgaatt gggcaacatc gacaaagccc
     4741 gcctgcgccc cgaattcaaa ttctaaaaca cagcaatatt ggagatgttc aaaatgagtc
     4801 gtgaaatgtt acagctggca gaagcactgg caagcgaaaa aaacgttgat gcggaagtcg
     4861 tcttccaagc actggaattc gcactctcta ccgccgccaa gaaaaaggca gaccgcgaac
     4921 acatggacgt gcgcgtccaa atcaaccgcg ataccggcga ataccaaacc ttccgccgct
     4981 ggctgattgt ggccgatgaa gactatacct atcccgatgt cgaaaaaacc atcgaggaaa
     5041 tccaagagga aatccccgat acgaccatcc aaatcggcga atactacgaa gagcagctgc
     5101 ccaacgaagg cttcggccgc caagcggcgc aaactgccaa acaaatcatc ctgcaacgca
     5161 tccgcgatgc cgagcgcgag cagaatctga acgagttcct cgccgtcaaa gaagacatcg
     5221 tgtccggcac ggtcaaacgc gtcgaacgcc acggcatcat cgtcgaagtc gttgccggca
     5281 aactggacgc gctgattccg cgcgaccaaa tgattccgcg cgaaaacttc cgcagcggcg
     5341 accgcatccg cgccctcttc ctgcgcgtcg aagaaatcgg caacaccggc cgcaaacaag
     5401 tcatcctgag ccgtacttcc ggcgatttcc tcgtcaaact gtacgccaat gaagtacctg
     5461 aaattgcagg cggcatgctt gaaatccgcg ctgtcgcccg cgacccgggc cagcgtgcca
     5521 aagtcgccgt caaagccaac gaccagcgca tcgatccgca aggcacctgt atcggcgttc
     5581 gcggttcgcg tgtcaatgcc gtcagcaatg aattgtccgg cgagcgcatc gacgttgtcc
     5641 tttggtcgcc cgagcctgcc caattcgtca tgagcgcgct ctcacccgcc gaagtcagcc
     5701 gcatcgtcat cgacgaagac aaacacgccg tcgatgtcat cgttgccgag gaccggctcg
     5761 cgctcgccat cggacgcggc ggtcaaaacg tgcgccttgc ttccgacctg accggctggc
     5821 agctcaacat catgacttcc gccgaagcag acgaacgcaa tgcggcagaa gatgccgcca
     5881 tccgccgcct gtttatgaat cacttgaatg tagatgaaga aaccgccgac gtactggttc
     5941 aagaaggttt tgcaaccttg gaagaagtcg cctatgttcc tgccgccgaa ctgcttgcca
     6001 ttgaaggatt tgacgaagaa atcgtcgata tgctccgcaa ccgtgcccgc gatgccatcc
     6061 tgaccatggc gattgctgcc gaagaaaaac tgggcgaagt gtccgacgat atgcgtaacc
     6121 tcgaaggcgt agatgccgat atgctcctca gccttgccga agcaggcatt accacccgcg
     6181 acgacttggc agagcttgcc gtggacgaac tgattgaaat caccggtgta aacgaagaaa
     6241 ctgcaaaagc cgtcatcctg accgcacgcg aacactggtt taccgaagac aaataaaggg
     6301 ggtacagatg agtaacacaa ccgtagaaca atttgccgcc gagctgaaac gccccgtcga
     6361 agacctgttg aaacagttga aagaagccgg cgtcagcaaa aacagcggca gcgattccct
     6421 gacgctggac gacaaacagc ttctgaacgc ctacctgacc aagaaaaacg gcagcaacgg
     6481 cggcaccatc agcatccgcc gcaccaaaac cgaagtcagc accgttggcg gcgtaaaagt
     6541 cgaaacacgc aaacgcggac gcactgtcaa cattccttct gccgaagaat tggcagcaca
     6601 agtaaaagcc gcccaaaccc aagccgcacc tgttcagccg gagcagacgg cagaagacgc
     6661 ggtaaaagcc cgagccgaag ctgccgcacg cgcagaagcc cgtgccaagg cagaagcgga
     6721 agcggcaaaa ctgaaagcgg caaaagcagg caacaaagcc aaacctgccg cgcagaaacc
     6781 taccgaagca aaagccgaaa ccgcacccgt tgcggcggaa accaaacccg ccgaacccaa
     6841 agaaaaagcc gtcaagccga aacacgagcg aaacggcaaa ggcaaagatg ccaaaaaacc
     6901 ggcgaaacct gccgcacctg ccgtgccgca acccgtagtc agcgcggaag aacaggcgca
     6961 acgcgacgaa gaggcgcgcc gcgccgccgc attgcgcgcc caccaggaag ccctgttgaa
     7021 agagaaacag gaacgccagg cacgtcgcga agccatgaaa caacaggcag aacaacaggc
     7081 aaaagccgca caggaagcca aaaccggcag acagcgtccc gccaaacctg ccgagaaacc
     7141 gcaggcagcc gcaccggccg tcgaaaacaa acctgtcaat ccggcaaaag cgaaaaaaga
     7201 agaccgccgc aaccgcgatg acgaaggtca aggccgcaac gccaaaggca aaggtgcaaa
     7261 aggcgggcgt gaccgcaaca atgcccgcaa cggcggcgac gagcgcgtac gcggcggcaa
     7321 gaaaggcaaa aaactcaaac tcgagccgaa ccaacacgcc ttccaagcac cgaccgaacc
     7381 cgtcgtacac gaggttttgg ttcccgaaac cattaccgtt gccgatttgg cgcacaaaat
     7441 ggcggtcaaa ggcgtggaag tggtcaaagc cctgatgaag atgggcatga tggttaccat
     7501 caaccaatcc atcgaccaag acaccgccct gattgtcgtc gaagaactcg gacacatcgg
     7561 caaacccgcc gccgccgacg atcccgaagc cttcttgggc gagggcgcgg aagcggtgga
     7621 agccgaagca ttgccgcgtc cgcccgtcgt taccgtcatg ggccacgtcg accacggcaa
     7681 aacctcgctg ctggactaca tccgccgcgc caaagtggta cagggcgaag cgggcggcat
     7741 tacgcagcac atcggcgcgt accacgtcaa aaccccgcgc ggcgtgatta ccttcttgga
     7801 caccccgggc cacgaagcct ttaccgctat gcgcgcgcgc ggcgcgaaag caaccgatat
     7861 cgtgattctt gtggtcgccg ccgacgacgg cgtgatgccg caaaccatcg aggcaattgc
     7921 ccacgctaaa gcggcaggcg ttccgattgt ggttgccgtc aataaaatcg ataaagacac
     7981 tgccaacccc gaacgcatcc gtcaggaact gacccaacac gaagtcatcc ccgacgattg
     8041 gggcggcacg gttcaattca tcgacgtttc cgcgaaaaaa ggaacgaaca tcgacgcgct
     8101 gctcgaagcc gtattgctcg aagccgaagt attggaactg accgcacctg tcgatgcgcc
     8161 cgccaaaggc atcatcgtcg aggcgcgttt ggacaaagga cgcggcgcgg ttgccacatt
     8221 gctggttcaa aacggtacgc tgaaaaaagg cgacatgctg ctggccggta cggcattcgg
     8281 caaaatccgc gcgatggtcg atgaaaacgg caaatccatt accgaagccg gcccgtccat
     8341 ccccgtcgaa atcctcggct tgtccgacgt accgaatgcg ggtgaagacg cgatggtatt
     8401 ggcggacgag aaaaaagcgc gcgaaatcgc cctcttccgc caaggcaaat accgcgacgt
     8461 gcgccttgcc aaacagcagg cggcgaagct ggaaaatatg ttcaacaata tgggcgaaac
     8521 ccaggcccaa tctttgtcgg tcatcatcaa ggcagacgtt cagggctctt acgaggcttt
     8581 ggcgggcagc ctgaaaaaac tgtccgccga cgaggtgaaa gtgaacgtgt tgcacagcgg
     8641 cgtgggcggc attaccgaat cggatgtcaa ccttgccatc gcttcgggcg cgttcattat
     8701 cggctttaac gtgcgtgcag atgcctcttc gcgcaaactt gccgaaaatg aaaacgtgga
     8761 aatccgctac tacaacatca tctacgatgc catcgacgac gtgaaggcgg cgatgagcgg
     8821 catgctttct cccgaaaaga aagaacaggt taccggtacg gtcgaaatcc gtcaggtcat
     8881 ctccgtttcc aaggtcggca acattgcagg ctgtatggtc accgacggcg tggtcaaacg
     8941 cgattcccat atccgcctca tccgcaacaa cgtggtcatc cacacgggcg aactggcttc
     9001 gttgaaacgc tataaagacg acgtaaaaga agtccgcatg ggcttcgagt gcggtctgat
     9061 gctcaaaggc tacaacgaaa tcatggaagg cgaccaactg gaatgcttcg acatcgtcga
     9121 agttgcccgc accctgtaat tcctttgcaa ataaaatgcc gtctgaagcg ttcagacggc
     9181 aacgaaacgg gttctgtatc atacagaacc cgttttttgt cgcaaatcgg cttcagcgac
     9241 cctcctgcct tatcccgatt tgaatctgac ttgccataca aacaggcttc agacggcatt
     9301 atttgcccgc caaacgtatc ccgatcttct ccgcatattc cctgcgttcg gcgcggctgg
     9361 tttccgggcg gtgcgtattg agcgacgacc atttccaatg actgcgggct ttgttgagtt
     9421 cgggcgggag tttggcggca tcccacgggg ctttgcggct gtgcagctcg atatccgact
     9481 gtgccgcgtg tccgcgcgtt tgcaggacgt ggagcagatc gagggcgcgg gcggcgagca
     9541 gggtcagggt ttcagggtcg gtgtgcaggg tttgacggcc ggcgagtttg tcggaaatgg
     9601 tgcgggtatt gggcaggatg ccgcccaaaa agccgccgat tgccgtaccc aatccgagcg
     9661 agccgccgag tgtggcgatg tccagcccca agccgatgag cgcgccggtt gccgcgcccg
     9721 tgccggtgcg gatgccgtat tgtttgagca attcgctgtc gaacgggtct tggcggaagg
     9781 cttgcggcat ccagtcgccg ccgtcgattt cgctgtggta gaaacggtaa agggcaaaca
     9841 gccgctgctg catctgccgt tcgagttggc gtatttccgc ctgcatggtt tgcagcacgg
     9901 tggcggtatc ctcgttttcg tccacttcct gcctgaaggc ggcggcatcg agcaggaaat
     9961 cggcgatttc gcggcgcgct tcgccgtcca gccgctgcca ttcgcgccgg cgcatggctg
    10021 tcaggcggtc aagtgtgctg cgttcgggca acatggtggc gaggttttcc cacaggcgca
    10081 attcgccttc aaaatcaaag gcgacggtgt cgaaccctgc gaaaacgtgc aggtttctcc
    10141 tcgccagcat ggttgtccac gattcgggaa gctgtccgcc ggtaaagttg aacacgggca
    10201 taaccggttt ggcacaccat gaaaggatgg tcaattcatc ccgatatttg tcgaggacgg
    10261 gttcgcgcgc gtcgatgacg tacattgcca tatcgctttg caaaacctgc cgcagcactt
    10321 tggcttcttg gttgaaatcg tgatgcgcgc cgtggctgcc gagaaactgt tgcagccgct
    10381 cgatgccgtc tgaacgattg tccgtatggt tttccagcca ttccaaaaca ccgcccgcgt
    10441 cttcgagtcc gggcgtgtca tacaggaaaa ccaacatgtc cgcgccgtcg ctgatggcgg
    10501 cttcttcgac atggcgtgtg gtggacggtg cgtttctgac ttcgccgaag ccgctgtcgc
    10561 gcaacagggt gcgcaggagc gaggttttgc cggtgttggt gtgtcccacg acggcgaggg
    10621 aaaggggttg tttgttcatg atgtttttga agaatggatt ttcagacggt cttttttcag
    10681 aatggcggct taacagaaca tttcaagtga gtttattggt ctttcaaacg gccttcctgc
    10741 gccaccctgt caggctcaag ccacgccgcg ccgcattcgg tcagcgcgtt acgccaatgt
    10801 tccagctttt ccgaaaggtc gtctgaaagc ccctgttccg ccaaaagctg caccaccgcg
    10861 ccgccctgcg ccgcttccga aagccgcaca atctgccgca gcacgccccg gtccggcaca
    10921 gtttgggcgc gtacgccgat aagcagttgc gccggtttct gcttcagctc tgtctccagc
    10981 gcggcaacct gttcccgatt ggcggcaacg cccttatcca gccattcctg cgccagcctg
    11041 ccctcgaacc attggccgtc ctgccactcg gtctccagca tgagcgccca tttcggcgca
    11101 tcgttcaaga cgattttcgg cgaaacggcg gacacggttt cccgacgcgt atccgcatcg
    11161 gtgattttgt tctgccagcg gcggatgacc gcctgataat aggttttttc caaatccaat
    11221 ccgttttcgc ttgttttcaa aaggatttta cacactaccc aagccaagag gcgcggcagg
    11281 atgccgtagc agacgatact gccgaccagc agccccgacc aagcccgcgc atcggcaata
    11341 ttgccgttca gacgaccttc gatgaccgcc cgcgcatcgg ggacagggaa accgagtttc
    11401 gacggcagcc atgccaacat ttccaccgcg cgtaccgaag cggcattgct caacagcgtg
    11461 ctttcccagt tgaacgtata ttgccgcacc aaaagcagca gcaataccga caccagcatt
    11521 ccgagcagcg tgcagagcca caagctgtgc gccgttgcgc ctattttcca tcgtaccgaa
    11581 ggttgccgcc actggtccgc atacagccgc aacaccgcct gatttacagg gcctttgccc
    11641 cgaaaccacg tcgccggact gctgaaaaac cgccccactt tcacgcgcag gaacaacgtt
    11701 gccaaccata ctgccagcat cagcgtattc atgcccaaca ctcccgccaa aactaaaaag
    11761 aaattcagcc cctgattgtc catcagaaga taagtgcctg aaaatccggc ggtaaacatc
    11821 atcgatgccg ccaccaccca taaccagaac gaccccgcac gcacacgttc caacgtgtcc
    11881 cgcagcatac ggtccctgtc gatcatctcc gcccgacgga tgattttttc ctccgtactg
    11941 ccgtccacgc ggcgcaaagc ctccgtcgcc tgcacgggat cgccgctgaa aataaaaccg
    12001 ccttcatcca aaatacggac cagctcaacc agttttcggg atggattcaa cataaaatgc
    12061 cgtctgaaaa taaaaaacaa attttaacac acgcattttc aagaatattc acagtgtagg
    12121 caaagagtaa atcccacaca gaggcaaaag tatcggcgta aactgactgc ctctactttc
    12181 ccgaaagatt gtgcgatgta tgcaggcgaa cgcttcaata cttacagcca tttgagcggt
    12241 ttgattctgg cggcggcagg tttgatgctg atgctgctga aaaccatagg acacggggac
    12301 ggctaccgta tcttcagcgt atcggtttac ggcatcagcc ttcttctgct ctatttgagt
    12361 tcctcgctgt accacggaat tgcagccgga aaactgaaaa gcattttgaa aaaaaccgac
    12421 cactgcatga tttatgtgct gattgccgga agctacacac cgtttgcact ggtttctttg
    12481 agaaacgggc cgggctggac ggtattttca ctgtcctggc tgctggcggc tgcaggaatc
    12541 gcacaagaac tcaccatcgg acggaaaagc gaaaaacgtc tgctgtctat tgcgatttat
    12601 atcgtaatgg gctggatggt cttggcggta atgaaatccc tgacagcctc actcccgccg
    12661 gcaggactgg cttggctggc ggcaggcggt atgctgtaca gcgtcggcat ttactggttt
    12721 gtaaacgatg aaaaaatccg acacgggcac ggaatctggc atctgttcgt attgggcggc
    12781 agcataaccc aatttgtcag cgtgtacggt tatgtaatct gaatgccgtc tgaaacacaa
    12841 aaacctccca aacctgaagg cagggaggtt ttctctttgc cgggcattat tgtttatcgt
    12901 ggaactcgtg gaattcatac tgataggata aatcctgtcc cgcctttttc cgcatcaggt
    12961 agtcatcata aatggcgcgg atttccttac gcagcaagaa taaggcaatc aggttgggga
    13021 taaccataaa gccgttgaac atatcggaca gattccaaac caaatcgact ttgccgagcg
    13081 tacccaaaac gatagcaagc agaaccaatg cgcgatagat gcccaagtgt cttcccctga
    13141 aaagaaaacg gatgttggac tcgccgaaat aataccagcc gataatggtc gtaaaggcga
    13201 agaaggtcag acacacggca agcaattgcg aaccgaagcc cggaaatgcc ttgttaaagg
    13261 caaattgagt aaccgccgcg ccctgttcgc ccgaaaggtt ggcatcggtc agcaggataa
    13321 tcaatgccgt agccgtacat accaaaatcg tatcgataaa tacaccgaca aatgccgtca
    13381 taccttgctg aacaggatgc ttcacatccg cagtcgcgtg ggcgtgcgga gtcgaaccca
    13441 tacctgcttc gttggaaaac agaccgcgcg ccacgccgaa acgtatcgct tcacgcatac
    13501 cgatacccgc agcaccgccc aaaacggcct cgggattgaa ggcggcggta aagatgtggt
    13561 tgaacatcgg cacaatatgg tcggaaaatt caaacaggat aacgacggcg cacaaaatat
    13621 aaacaaccgc cataaacggc acgacaaacc gggcaatatt ggcaatacgg ttcacgccgc
    13681 caatcacaac catgcccgca aggacggcaa acacaatacc gactgccaaa gaaggcacat
    13741 caaatgcaat ggtaacggca gaagcgatgg aatttgcctg agtcgcattg ccgataaagc
    13801 ccaacgcgac aatcagcgcg atggagaaaa agcccgacag gaaacgcgcc gcgcccctgc
    13861 cgattttcgg agtcagaccg tgcgtaatat aaaacgccgg cccgccgatg tatttgccgt
    13921 ggctgacgac gcggtatttc tgcgccagca gtgcttccgc aaaaatcgtg gacatcccca
    13981 aaacggcaga aagccacatc caaaaaatcg cacccggccc gcctgcggta atggcggtcg
    14041 ccacaccggc gacgttgccc gtaccgattt gcgcggatat agcaaccgcc aacgcctgaa
    14101 actgcgataa ggacttatcg tctttatcac ctttggcaaa caagccgccg aatacggatt
    14161 tgaatcccgc gcccagcttg gtaatctgcg gcgcaccaag atacagcgta aaaaacaggc
    14221 cgatacccaa aagcgcataa atcagcaggt agccccaaag aaaccgattg actgtaccca
    14281 ccagaacaga caatatattt tccataaaat aaaccttatc ttacgattaa aatgactgtt
    14341 ttccaaaaga cattccaata aggaaacacg gcgagcagac cgtatttgcc gcaacagatg
    14401 ccttaaattg tcaataatcc gagagaagct gtgccagcat accgtaaaat atcgttaatt
    14461 aaaatatttc tttattttta agcggaaagc ggaggaaatc gttttcagac ggtatcgaca
    14521 acggcacggc ataaaacagg atattttggg tacttgcaac ttatgttaaa atgccgaccg
    14581 taaaaaatct gacaaaaaca gattaattat ttgaaataag aaaggaaatt tatggcaggc
    14641 catagcaagt gggcgaatat ccagcataaa aaagcccgtc aggatgccaa acgcggcaaa
    14701 atcttcaccc gtttaatcaa agaaatcacc gttgcggcgc gtatgggcgg cggcgatccc
    14761 ggcgcaaatc cgcgcctgcg tctggctttg gaaaaagcag ccgaaaacaa tatgcccaaa
    14821 gacaatgtgc aacgcgccat cgacaaaggt acgggtaact tggaaggcgt ggaatacatc
    14881 gagttgcgct acgaaggcta cggcatcggc ggcgcagctt tgatggtgga ctgcctgacc
    14941 gacaacaaaa cccgcaccgt tgcggacgta cgccacgcat ttaccaaaaa cggcggcaac
    15001 ttgggtaccg acggctgcgt ggcgttcaac ttcgtgcatc agggctattt ggtattcgaa
    15061 cccggcgttg acgaagacga gctgatggaa gcggctttgg aagccggtgc ggaagacgtg
    15121 gttaccaacg acgacggttc catcgaagtc attaccgcgc caaatgattg ggcgggcgta
    15181 aaatccgctt tggaggcggc aggttacaaa tccgttgacg gcgacgttac gatgcgcgcc
    15241 caaaacgaaa ccgaactctc cggcgacgat gccgtcaaaa tgcaaaaact gattgacgcg
    15301 ctggaagact tggacgacgt gcaagacgtt tacacttccg ccgtattgaa tctggactga
    15361 tacgcagcac agcagacata caacaaaatg ccgtctgaac ctttcagacg gcattgattt
    15421 atttagccct tgcccacgcc caccaaaccg tcaggacaac cgccaaaaaa tacgccgcgc
    15481 tccaaacagg caaagcaacc cctaacagat aatccggttc ggcacaattt ccgaacccgc
    15541 gcacaacagg ctcgaaccaa tcaaacaaag gccagccctt caatcgaaac gtccacggcg
    15601 cgccgcacga aggagccgca cccggcggca gcgactgcaa ccacaactga tatgccgcaa
    15661 cagaaatacc cgtaacggcg ggaatactga taaacactgc gcctgacagt ccgcccaccc
    15721 tgcccttggg cttacacgcc agcacaactg ccgcacacaa tgcggttgcc aaaacacata
    15781 accgctgact gatgcacaaa acgcaaggct ccatacccaa aacatactgt gccgccaaag
    15841 aaccggcaaa tgcacagacc gaaacggcaa acagcagcca aacggctttt ctaaataacg
    15901 gggtcatttt ctcaacacac caatcaaaat accgatatgc cgattttgct ggatatatcc
    15961 cgagatggca agggacgaat cgacaccggc acaggtgcgg acgatttcgg ttttcccgat
    16021 atgcacaaaa cctgacaaag gacaaaacgg cgatttgtcc gcacaatgcc cgaaaaaata
    16081 catccggagg atttgaattt gaatttcggc aaatttagcg aatcaaaagc aacagccgat
    16141 aaaaaaatca aaagcttatt tcaatgtttc aacacacagg acgacacata aagcaccgcc
    16201 ctatgtgccg tcctgatttg gaaggggtta cacccctccc aaatatagtg gattaacaaa
    16261 aaccggtacg gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgccgcc
    16321 ttgtcctgat ttttgttaat ccactataaa gtctgattct accgccctaa agggcagggt
    16381 ttcaaccgaa aaggaaacac gatgaaagca tatgattttt tgaataatcg gattgacggg
    16441 tttttgaggg aatttgttac cggatagcca tcgggcaagt tttttgcaaa atttgaatcg
    16501 gtcgggtaaa ttttcaaaaa ataattgaca gcagataaga aacggcggat aattaccgcc
    16561 gttgagttgc ttgatgcagc ttgatttttc tcctctactt ctcctttgta gacttggcac
    16621 acattcaacc ggatgtgtgc attttttatc tccgcttttt ttgaaattta attactttta
    16681 ttgtttgaaa ttctattctt taagaaattt aacaagaaaa acgcttgctt tttgaccgaa
    16741 aaagctgcat aattcgttcc gttagctggt ttgagtagtc agttaatagt ttctcctcta
    16801 tttctccttt gtagacttgg cacacattct attgaatgtg tgcatttttt atctgaagca
    16861 acaagcctct gtgcgtgatg ttgttatgtt tcatttagat gtcaaaccgc atacccggtc
    16921 tgaaatattc aatccaaatc caaaaccgga ttttctttga cttcctccat cacaacataa
    16981 ctcctactct ccgaagcggc aggcagttgc aataggatat tgccgagcat atcccgatag
    17041 gcagacatat cgggcaaacg tactttaatc aggtagtcgt attcgcccga caccagatgg
    17101 cactccataa tttgcggaat tttcagcact tcttttttga aatcttcgaa aatattgccc
    17161 gatttggatt gcagcttcag ctcgacaaaa accaatggcg gttttcccaa cagatgggga
    17221 ttgagatggg cgtgatagcc ggaaatataa tgttcccgct ccaaacggca taccctctct
    17281 gtaacgggcg tgctggacaa ccctaccttc tcggcaagct ccgtcatcgg gatacgggca
    17341 ttttgctgaa gaattttgag gatgcggaaa tcgattttat ctagttcttt catttggatt
    17401 accttgtatt tattattgat tttaacaaat agagtataaa tttgggaaag caattatatc
    17461 aggaaaagca aaccgccttc ctacctgaaa actgctgctc tggcttgaag acacaaggtt
    17521 ctttaatatt ttaaaagcct tgccgttgga ttataattcc ccaattgatt tcttaatttt
    17581 gctaataaac acttgtttgg taaggaatga atttatgcgg cctttgaacg tgcagatcag
    17641 gttgggcaac cttaggcaca attatcggat tttgaaggaa atgcacggag gcaagctgtt
    17701 ggcggtagtg aaggccgacg catacggaca cggtgcggtc agatgtgctt tcgcgctggc
    17761 agacttggca gacggctttg ccgtggcgac aatcgacgag ggaatcaggc tgcgggagag
    17821 cggcattacc catccgattg tccttttaga aggcgtattt gaagcatcag agtacgaagc
    17881 ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa tggcagcttg aggctttgct
    17941 ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa atggattcgg gaatgcaccg
    18001 taccggattt ttccctcatg attacgcttc ggcatatgcg gcattgaaac agtcggaata
    18061 tgtggacagt attgtcaagt tctcgcattt ctcctgtgcg gacgaacctg aaagcggtat
    18121 gacggaaata cagatggaag cattcgattt gggtacgaaa gggctggaag gcgaagaaag
    18181 ccttgccaat tcggcagcta ttttgaatat ccccgaagca cgcagggatt gggggcgcgc
    18241 gggcttggcg ttatacggca tttccccgtt cggaggcagc gatgacaggc tgaaacccgt
    18301 gatgaggctt tcaacccgta ttttcggcga acgcgtttta cagccgcact cccctatcgg
    18361 ttatggcgca acattttata ccagtaaatc tacgcgcgtc ggtctgattg cctgcggtta
    18421 tgcggacggt tatccgcgcc gcgctccaag caattccccc gtcgccgtcg acggtaaatt
    18481 gacccgggtc atcggcagga tctctatgga tatgatgact atcgagctgg acgcttcgca
    18541 agaaggtttg ggacacgagg tcgaactgtg gggcgatacg gtcaatatca ataccgttgc
    18601 cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat atcaaacgtg caaaattcac
    18661 ttatatcgag taatcgagtc caaacgaaaa tcccgtctga agcttttcag acggcataaa
    18721 tgcgttttga caaaatcagt aaaagacatt gccaaataga aaatactgat aatgcccaca
    18781 atctgaaacc ccatccttcc cgcaaaaacg gaaaatcaaa atcagaaacc taaaatttcg
    18841 tcattcccgc gcaggcggga atccggtttt ttgagtttcc gtcatttctg ataaattctc
    18901 gtcgtttttc atttctagat tcccgcatgc gcgggaatga caaaattaaa gtttcagaat
    18961 ttatttgaac aacaaactag attcccgctt tcgtggggat gacggcggat aggttttgtt
    19021 gtttcggata aattcctgtt gttttttggt ttccagattc ccacctttaa aagaatgacg
    19081 taagaaagca gaaataagaa cacggggatt ttttaaattt gcaatattct tttctaaaga
    19141 cattttaccc aatgcccata aacaaaaatg ccgcctgaag cctttcagac ggcattttcc
    19201 catcaaaacc gcaatcagtt tttcatcgat tgaaccggtg cgggaatcct gccgccccgg
    19261 ttgacgaaca cttcgcacga accttctttt gccggcatta caggcgcgta gcccaacaga
    19321 ccgccgaact cgacgctgtc gccgacggtt ttgcccgtta ccggaataat gcgcacggcg
    19381 gtggttttgc tgttgatcat gccgatggcg gcttcgtcgg cgatgatgcc ggaaatggtg
    19441 tgcgcgggcg tgtcgccggg aacggcaatc atgtccaaac caacggagca gacggcggtc
    19501 atggcttcga gtttgtccag cgtcaacacg cctgcctcgg cggcggcaat cataccttcg
    19561 tcttcggaaa cggggataaa cgcgccgctc aaaccgccga ccgcgctgga agccatcatg
    19621 ccgccctttt tcacggcatc gttcagcaat gccaaagctg ctgttgtgcc gtgcgtaccg
    19681 cagacgctca agcccatttc ttcaagaatg cgcgccaccg agtcgccgac ggcgggggtc
    19741 ggtgccagcg acaaatcgag aatgccgaac gggatattca gcatttttga ggcttcgcgg
    19801 ccgatgagtt cgcccacgcg ggtgattttg aaagcggttt tcttcacgac ttcggcgacc
    19861 tcggtcaggc tgaccgcgtc cgaattttcc agcgcggctt tgaccacgcc tggaccggat
    19921 acgccgacat taatcacagc atccgcttcg cccgagccgt ggaacgcacc cgccataaac
    19981 ggattgtctt ccaccgcgtt gcagaacacg acgattttgg cgcagccgaa accttcgggc
    20041 gtgatttcag ccgtgcgttt gatggtttcg cctgccagct tgaccgcatc catattgata
    20101 ccggcacgcg tgctgccgat attgatggag ctgcacacga tatcggtagt tttcatcgct
    20161 tcgggaacgg aacggatcaa cacctcatcc gaaggcgaca tacctttttg caccagcgcg
    20221 gaaaagccgc cgataaagga cacgccgatg gctttggctg ccttgtccaa agtctgcgcc
    20281 acgctgacat aactgtcggc tttggtcgcc gccgcgattt gggcaatcgg cgtaacggaa
    20341 atgcgctgat tcacaatcgg cacgccgtat ttggcggaaa ggtgtttcgc cgttgccacc
    20401 aagtctttgc cgaccgtggt gattttgttg taaatgtttt ggtttaacac gtcgatgtcg
    20461 gtgctgatgc agtcgtgcaa atcaatgccg atggtaatgg tgcggacatc aaaattctgg
    20521 tcggcaacca ttttgacggt ttctaaaatt tcgccggatt ggatactcat cacattcctc
    20581 cgactcaaat gcggtgcatc gcttggaaga tttcttcgtt ttgcatacgg atgtcaagcg
    20641 cgagtttttt gctctcttcc gcaaacaaat ccaaaatctc ctgccgcgat ttagggcatt
    20701 tcgaggtatc caccaagata atcatagtaa aaaaatcgtc catcagctgt tggctgatat
    20761 tgaggatgtt gattcggttt tccgccaaaa ttttggaaac atcgtacacg atgccgacgc
    20821 ggtctttacc gatgacggtg atgactgaat tgttcacagg cttactcctt gcagatatcc
    20881 gttaaaatcc gaaattatac caccgtcgga ttttgaagaa tattgtcaac aatatataca
    20941 tacaaaatgc cgtctgaaac tatttcagac agcatcaaga ttcagggttc gattaaataa
    21001 ccatccttat cccattgggt tttcctgacc aacttgtcat cctgataaac agcctcgctc
    21061 tttttagaac catcttcata ccactccaaa accaccccgt tgcgtttatg gtggcggata
    21121 gacagttccg aaagtaatcg gccgctttca tcccaagtca gaattttggc aggctcatcg
    21181 ttgaccataa ccatttccgt cttgatactt ccgtcggcat accattgctt ccatacgccg
    21241 ttcgccttat tttgcttaaa ctggatttcg ctttccttgc cgccgttacg gtaataacgg
    21301 tatcccgtac cctcactcaa gccattttta taaggcataa ccgcagattt tttaccgttc
    21361 ggataccagt tgacccattc cccgtccggc ttacccttgc tgaagccccc cgccattttt
    21421 ttctgaccat taaaatgcca caaaatcaac ataccgtttt gcagggtagg cacaaaagat
    21481 ttgatttgcg ttgaagcaac gatataaggt tcggaatatt tcttcatcga cggataataa
    21541 aaatcctgcg cgtgtgcgat acccgccgcc acactatatt gcctgatata agcggcagaa
    21601 gacatcgtcg ccgtcagttt cccgttctga ttaaaataaa cagaataggt ctgcgccggc
    21661 aaagcggccg aaaaacccaa cagtacgatt gaaaatacaa tccgagataa tttcttcatt
    21721 atgatagcga tataaaaaac aattaactta taaacaatat ggtaaggatt tctcaaccaa
    21781 gcgtcaaacc cgaaacaatg tatcgtaaaa atgccgtctg aaaacaaatc gtcttcagac
    21841 ggcatttccc tccaactcac tcttcgccca ataactgctc gcgcgtcaag aggaaaacaa
    21901 aaccgtcgcc cccgctggtt tccaaccaag taaaaggcaa ctccggatac gctgcttcca
    21961 atacatccct gttatgcccg atttccacca gcaatacacc tttaggattc agaaactttg
    22021 ccgcattcag aagaatctgc ctggtggcat ccaacccgtc cgccccgctg cccaacgcca
    22081 attccggttc gtgcaaatat tcctcaggca aagccccaac cgactctgca tccacataag
    22141 gcggattgga aacaatcaaa tcataagtgc cttccagtcc ctcaaacaaa tcggtatgaa
    22201 taagctgaat acgctcttcc agaccataat cttcaatatt gatccctgca acttccaaag
    22261 catccaaact cacatcaacc gcatcaattt gggcatcagg ataatggtgc gccatctgaa
    22321 tggcaaggca accgcttccg gtgcaaagat ccaaagcatt atgcaccagc tcatcgtatt
    22381 ctatccaagg tcgaagtccg tcacccagca attcataaat aaaagagcgc ggtacaatca
    22441 cgcgttcatc tacataaaaa tcaaactccc cctgccaggc ctggtgtgtc aaataagcgg
    22501 caggaatgtg ttcgacagca cgacgctcaa taaccgccag cacttcctct ttttcagctt
    22561 ccaagagttt tgcatcaaga tatggggcaa gcatatccaa aggcaaattc aaagtatgca
    22621 gaatcaaata aaccgcttca tcatgcgcat tatctgttcc atgaccaaaa aagagccctg
    22681 cctcattaaa acggctgact gcaaaacgta aaacatcacg gatagtcgtc aattcttgtg
    22741 cggcctgatt aaacataata tgaaccattc tgcgtataga tgcttttaat tataacagaa
    22801 acaacaagca aaccttttca tatcgccaaa taaccactca atctacccat acaactacat
    22861 aaatgccgcg cgaagaccat tgcccgaacg gaaacggcga tggcggacgg tatgggcaac
    22921 ctgatagact gggaagaaac ggggcttgtt gagggcaagc agtggataac cgcaaaagac
    22981 gacaaggtgt ccgatgtctg caatgccaac ggcgggatgg gcgtaatcgg tttgcacgag
    23041 cctttctccc acggtgcgtt gacgataccg gagcatccaa atggcaggtg cgcggttgtt
    23101 cccgttgtcg ggcggacagg caagctggcg aaaggcagcc gcgcgatgga aacgggcgat
    23161 ggacggcagg tgaggttgtt tccgtatcgg gcggcgaatt gggggaattt gctgaaacaa
    23221 aggagcttcg taaagcggct atgcagtatg cgcgtgataa ctttgtcggc aaaagctatg
    23281 tcaatgaaag cagcgggcat gaactgaagg taacttggca aggtgtgaaa cacgctgcgt
    23341 caaaatcgaa cagcatcgaa ctttccctta tggtcgcgct tgataaagtt ttgatgaatg
    23401 caaaatatga aggaagtttt cctaacaaat tagggaggga tcatattatt gcggtacaca
    23461 aatactcaac cggtgtcaga tttggcaata aagtcttaaa aatcaggata atagcccgtg
    23521 agaaatttga tgggataaaa cattacgatc attttatttt gaaagacaaa tgaaaaaacc
    23581 ctgtccagta tatctgggat agcttatgga agcatttaac ccagcctcgg ataaggcttt
    23641 aaatccatca tgccatttat acggtaaaaa caagggtctg tactagatta gcagatatgt
    23701 taccctcgaa atatgaagat aacacactgc aaattaaaga aagaagtaca aaaagaaccg
    23761 ctccgttctt ttgtaccgga agttaccgcc cgttctgccg ccgatatttt gggtatccat
    23821 cccgattcgg cggcactgtt ttaccgtaaa atccgcacgg ttaccaacca tcatttggcc
    23881 ttggctgccg atgaggtttt cgagggcccc gccgggccgg gcggaagc
//
LOCUS       DAXFWM010000025        23785 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000025 DAXFWM010000000
VERSION     DAXFWM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23785
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.025"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(148..327)
                     /locus_tag="ACFI8B_000980"
     CDS             complement(148..327)
                     /locus_tag="ACFI8B_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC3834235.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(333..920)
                     /locus_tag="ACFI8B_000981"
     CDS             complement(333..920)
                     /locus_tag="ACFI8B_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC3834236.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(942..1688)
                     /locus_tag="ACFI8B_000982"
     CDS             complement(942..1688)
                     /locus_tag="ACFI8B_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC3834237.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSPPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(1678..2520)
                     /locus_tag="ACFI8B_000983"
     CDS             complement(1678..2520)
                     /locus_tag="ACFI8B_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC3834238.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(2638..2946)
                     /locus_tag="ACFI8B_000984"
     CDS             complement(2638..2946)
                     /locus_tag="ACFI8B_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834239.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(3020..3469)
                     /locus_tag="ACFI8B_000985"
     CDS             complement(3020..3469)
                     /locus_tag="ACFI8B_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC3834240.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKLFCPKCRLKQD"
     gene            3810..4703
                     /locus_tag="ACFI8B_000986"
     CDS             3810..4703
                     /locus_tag="ACFI8B_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC3834241.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            4806..5909
                     /gene="prfB"
                     /locus_tag="ACFI8B_000987"
     CDS             4806..5909
                     /gene="prfB"
                     /locus_tag="ACFI8B_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC3834242.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            5988..6347
                     /gene="rnpB"
                     /locus_tag="ACFI8B_000988"
     ncRNA           5988..6347
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFI8B_000988"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6678..8135)
                     /locus_tag="ACFI8B_000989"
     CDS             complement(6678..8135)
                     /locus_tag="ACFI8B_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3834243.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGVWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(8917..13086)
                     /locus_tag="ACFI8B_000990"
     CDS             complement(8917..13086)
                     /locus_tag="ACFI8B_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC3834244.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(13141..14988)
                     /locus_tag="ACFI8B_000991"
     CDS             complement(13141..14988)
                     /locus_tag="ACFI8B_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC3834245.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            15360..16589
                     /gene="sstT"
                     /locus_tag="ACFI8B_000992"
     CDS             15360..16589
                     /gene="sstT"
                     /locus_tag="ACFI8B_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC3834246.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            16740..18020
                     /locus_tag="ACFI8B_000993"
     CDS             16740..18020
                     /locus_tag="ACFI8B_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3834247.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPYP
                     SGGRFAAKVDFGSKSVDGIIDSSDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            18020..18223
                     /locus_tag="ACFI8B_000994"
     CDS             18020..18223
                     /locus_tag="ACFI8B_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC3834248.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            18283..18531
                     /locus_tag="ACFI8B_000995"
     CDS             18283..18531
                     /locus_tag="ACFI8B_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834249.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(18524..18739)
                     /locus_tag="ACFI8B_000996"
     CDS             complement(18524..18739)
                     /locus_tag="ACFI8B_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834250.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(18732..18947)
                     /locus_tag="ACFI8B_000997"
     CDS             complement(18732..18947)
                     /locus_tag="ACFI8B_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC3834251.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(19150..20493)
                     /gene="argG"
                     /locus_tag="ACFI8B_000998"
     CDS             complement(19150..20493)
                     /gene="argG"
                     /locus_tag="ACFI8B_000998"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC3834252.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            20618..21418
                     /locus_tag="ACFI8B_000999"
     CDS             20618..21418
                     /locus_tag="ACFI8B_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC3834253.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            21458..22444
                     /locus_tag="ACFI8B_001000"
     CDS             21458..22444
                     /locus_tag="ACFI8B_001000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC3834254.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            23105..23356
                     /locus_tag="ACFI8B_001001"
                     /pseudo
     CDS             23105..23356
                     /locus_tag="ACFI8B_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23354..23550)
                     /locus_tag="ACFI8B_001002"
                     /pseudo
     CDS             complement(23354..23550)
                     /locus_tag="ACFI8B_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            23652..23785
                     /locus_tag="ACFI8B_001003"
                     /pseudo
     CDS             23652..23785
                     /locus_tag="ACFI8B_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         5326 a         5904 c         6936 g         5619 t
ORIGIN      
        1 agccttcgtg ttttacgtat ttatatattc cgtatggagg aatatgctgc ttttgaagag
       61 tgtattatgg atgaactgcc gataacagta aatcggtttg atttgtttcc gtcagcttat
      121 gccgtctgaa gccctttcag acggcattta ttttgaagtt ctggttgttt ttgccagtga
      181 tattttgatg gtttgaagct gtcttttata ttcccggcaa tgcgggcaga acagcaggtg
      241 catatatatg gaaatcttct cgcccggggt ggtttcccgg tcctgatgtt tggaaagaag
      301 cagggcaata tcgcggcatt ttttcataac gtttacttcg ggttttcttg gttgaaccat
      361 ttgatttgca ggcattggcg caatgattct cgggcgcggt gcataatggt gtggtagttg
      421 gacgtgctga taccgcacat ttgttgtatt tcgttggatg aaaaaccgag tatttccttc
      481 agggtgaata cccgtgcggt gttttcaggc aggttgtata ggcagctttg cagaattttt
      541 tggaattcgt tgttatttaa tgatttttcc ggagtgttcc aatgttgcgg ctgcccttcc
      601 ggcgtccaat gcccgttttg ggaaaaatgg ctttcaaatg cttcatccag cagctcgtca
      661 tccagtgcgg taaagacttt cctctgcctc ccgatttgac gtaatgcgtc aataattttg
      721 tttttcaata tggcaaaaag ccagctgttg acaagtgccc tgccttgaaa actgtcgcct
      781 gcgctgtatg cggacagcaa tgtttcctgc actaaatctt cagccaaatc agggtggtcg
      841 ggcaactgga gtcgcgcaaa atgcagaagc agtttgcgcg actccattaa ttcggcatcg
      901 gtcaggtcgg gtagcggcat agtatcggga acaaggtaat tttatgcgga tagatggttt
      961 tggctcgggg aattgccttc catattttcg gatatggcgg acaaggaggg gatgataatc
     1021 ctttgttcaa tccagcctga ccatttccca agcaaaatat ttttccaatt atcggcttta
     1081 ggcataaatt cgacaagggt ttgggcgagg gtgtcaaacg aaagcgcgga ggaacccatt
     1141 atctccagca gcatcatatc gaagccgtcc aatgtttggt acatcacatc atcttcggcg
     1201 tttcgccata ttaacaaggc tgtttccgct tcctgcaaat catgggtaac atcgtatcga
     1261 tattgccgga taaaggccgc aggggagggc gtgtatttgc tgtcatttga ataatgaatg
     1321 tcgggaatat ccggaatttg tgcaacttct gccagcaatt gggtatattc aaaatccatc
     1381 agcgccaaaa tgccgtctga aagcggcggg ctttggcaat attgtaggaa ctcgccgggg
     1441 atttcttgaa aatagggcgt ttgggcacgc gcgtcgcgga cgaaaccttc tttcagacgg
     1501 ctccattctt tgctgtcaaa gtattgccgc gtttcggtat agcagcggtc gataaagctg
     1561 tggatattgt tgcgtatcag gcggatatag acgttcaatc ggtcttgcgg cagaccgtct
     1621 gcggcttcgc ccccgcgtat ggcttgggag aaacggtgct ggtattgggc ggaggtttca
     1681 ggctgcacgg cggtattcct ttccggcacg cgtttgataa tcggcaattt tggcaacttc
     1741 ggcttcgagt tcggcaaaag gtgggaaatt gaaatcgcgt tccaacaggg tgggagggat
     1801 ggtgggaagc ttggtgtagg cgagttcgag caagtcccaa acggtcggta aaaccgccgc
     1861 gccgtgggta tcaatcaaaa gctcgggggt ttcggcatcg tgtccggcga tgtggatgta
     1921 gcacacgcgc cctgcgtcca cattctctaa aaaggcctca ggcgacagca ggccgtgatt
     1981 gacggcgttg acgtaaatat tgttcacatc caaatgaata ccgcagtcgg cttcacgggc
     2041 aaccgcgttg aggaactcga cctcgttcat ctcggcaagc ggagaatgca ggtagtagga
     2101 cgtgttttcc acggcgatgc ggcagcccaa acggtcttgc acttcgcgga tacgccgcgc
     2161 cgtatgatgc accatttctt cagtgaaagg cagcggcaac aaatcgtaaa gatgaccgcc
     2221 gtcgtggcag tagctcaaat gatcggagaa aaacgtgcag tcataacggc acatcatttc
     2281 tttgatgccg tctatcaaat cggtatccag cggggcttgt ccgcccagcg acatagataa
     2341 tccgtgcaac gccagcggca gccgttccgc cacacggtca aactgtttgc gcgccctgcc
     2401 gcccattttc agccagtttt ccggtgcggc ttcgataaag catatcgggc tgttttcgga
     2461 aagcgagaga aagtcttccg ccaaatcgcg gcggtagccc aagcctgcgt gttgaatcat
     2521 atttttaccc ggttcggatg gcacaaaaga atgatgtgct tgttaaaaaa aatctttgtt
     2581 tgccgttaaa aaagccgcat tttactgaaa aacgcggctt ggtttgaagg ggcgggatta
     2641 tttagaaccg catttgcctt cgccgcattt gccttcggca gatttggctt tggctttaga
     2701 tgctttggtg tgtttgtggg ctttttttac agttgcaccg catttgccct cgccgcattt
     2761 gccttcgcct gctttagaag cagccgcgcc gcacgaacct tcggcagatt tggatgcgcc
     2821 gcaagagcct tgggcggatt tttgaacgcc tgttgcgttg cttgccggtt tgtgggcggc
     2881 aacggcgccg gcggccagag acagggataa agcaccggcg agtgcggcag caatattttt
     2941 gttcatttct atttcctttt cagaatatca agttaacgag ataaggttgg aatgtttaaa
     3001 gtcggattcc acgactgaat caatcttgtt tcagacggca tttggggcaa aacagttttt
     3061 tcagcagcgt atccagcgac catccgcccg caccctggaa aagcagcggg attaatacca
     3121 cgatataaat taaagccatt ttataaccgt tgtcgcagac attgtaaccc gaaccggcgt
     3181 gaaccgcagc ccaagcgacg gcggtaacga ccatcagccc caatgccgac aggcgcgttg
     3241 ccaaacccaa aagcaacagt acgggcagca aaagctccgc atacatggcg aaattccagt
     3301 ttaacgcgtc cggcagcaag ttgaacggga atggaaactg atcgttgatt tcggaaaacc
     3361 aattctcccc gttccatttt tgcaaacccg attccaaaaa ttcatatgcg gcaaacagac
     3421 gcaaaaccag catagcggat gccgtctgcc aagttgttcc gattttcata agtgttcctg
     3481 tttttgttcc tgttgatacc ccgttagacg gattttgtcg gaaaaactga cacccgtcag
     3541 ctaataattt aacaagttaa tgaatataaa aagaaagtat tttgacggaa agaaagggaa
     3601 aaaggcggga tatttggata acggcggtgt tgtgagataa ttcccgcctt ggacggtatg
     3661 ccttcaaata caatttccgt aaaggaaaaa aagaaaatat taattttgat gatgacttaa
     3721 caaagcccct ttcgcttggc tgtcaaagga gattgttaag aaaagcaatc tccctttgac
     3781 ggggtgcgat atgtaaagga atggaaaata tggtaacgtt ttcaaaaatc agatcatttt
     3841 tggcaatcgc cgccgccgcg ttgcttgccg cctgcggtac ggcgggaaac aatgccgccc
     3901 gcaagccggt gcaaaccgcc aaacccgccg cagtggtcgc tttggcactc ggtggcggcg
     3961 catctaaagg atttgcccat ataggaattg ttaaggtttt gaaagaaaac ggtattcctg
     4021 tgaaggtggt taccggcaca tcggcaggtt cgatagtcgg cagccttttg gcatcgggta
     4081 tgtcgcccga ccgcctcgaa ttggaagccg agattttagg taaaaccgat ttagtcgatt
     4141 taaccttgtc caccagtggt tttatcaaag gcgaaaagct gcaaaattac atcaaccgaa
     4201 aagtcggcgg caggcagatt cagcagtttc ccatcaaatt tgccgccgtt gccactgatt
     4261 ttgaaaccgg caaggccgtc gctttcaatc aagggaatgc cgggcaggcg gttcgtgctt
     4321 ccgccgccat tcccaatgtg ttccagccag tcatcatcgg caggcacaaa tatgttgacg
     4381 gcggtctgtc gcagcccgtg cccgtcagtg ccgctcggcg gcagggggcg aatttcgtga
     4441 ttgccgtcga tatttccgca cgtccgagca aaaatgtcgg tcaaggtttc ttctcttatc
     4501 tcgatcagac gctgaacgtg atgagcgttt ccgtgttgca aaacgagttg gggcaggcgg
     4561 atgtggttat caaaccgcag gttttggatt tgggtgcagt cggcggattt gatcagaaaa
     4621 agcgcgccat ccggttgggc gaggaggcag cacgtgccgc attgcctgaa atcaaacgca
     4681 aactggcggc ataccgttat tgacgctatg ccgtccgaac gtatttccag aaggcttcat
     4741 ccatagaaaa aaatacccga atcccgtaca atacgcccct aaaattttga aagaacgaaa
     4801 gaatcatgga agccgaagta atcaaccagc tcaacaacac cctgaatgat ttggagaagc
     4861 gtagcgaaga catccgcgtt tatatggatt atcagggcaa gaaagaccga ttggaagaag
     4921 tcatcgggct ttccgaagac cccgagcttt ggaacgaccc gaaacgtgcc caagaaatcg
     4981 gcaaagagag caaaatcctt gaaggcatcg tgctgacgct cgacaacatc gcttcgggta
     5041 tcgaagacaa ccgtatgctg attgaaatgg ccgtcgaaga aaacgacgaa gaaggttttg
     5101 ccgccgtgaa ggaagacgtg gcggggctgg aaaaacagat ggcggatttg gagttcaaac
     5161 ggatgttcaa ccagcccgcc gacccgaaca actgctttat cgacatcacc gccggcgcgg
     5221 gcggtaccga agcggaagac tgggcgggga tgttgttccg tatgtacagc cgctatgccg
     5281 agcgcaaagg cttcaaaatc gaaatccttg aggaagacga cggcgaaatc gcaggcatca
     5341 accgcgccac catccgagtg gaaggcgaat acgcttatgg tttgctgcgt accgaaaccg
     5401 gcgttcaccg cttggttcgc tactcgccgt ttgattcgaa caacaaacgc catacctcgt
     5461 ttgcctccgt gttcgtttac cctgaaatcg acgattccat cgaaatcgaa atcaaccccg
     5521 ccgatttgcg catcgacacc tatcgcgcat cgggtgcggg cggtcagcac atcaacaaaa
     5581 ccgactccgc cgtgcgtatt acccacgagc cgacggggat tgtggtgcaa tgtcaaaacg
     5641 accgttcgca acacgccaac aaagcggccg cgatggaaat gttgaaatcc aaactgtatg
     5701 aattggaaat gcgcaaacgc aacgaagaga aacaggcatt ggaagaaggc aagtcggatg
     5761 tgggttgggg cagccaaatc cgttcgtatg ttttggattc ctcacgtatc aaagacttgc
     5821 gtacaggcta cgaagtcggc aacaccaaag ccgtattgga cggcgacttg gacggcttta
     5881 tcgaagccag cctgaaacag ggtgtgtagt ttaggctgta tgccgtctga aacctgtacc
     5941 gtttcagacg gcatttttgc gtttcgtgtt ataatccgcg ccgcacacgg gacgggcaga
     6001 cagtcgccgc gtatcgcgta aggcatacgg ggaggaaagt ccgggctccg cagggtagaa
     6061 tgccggttaa cggccgggcg cggtaacgcg acggaaagtg gaacagaaag caaaaccgcc
     6121 gatggctgct ttggcagcac aggcaagggt gaaaaggtgc ggtaagggcg caccgcgcat
     6181 ttggtaacaa tatgcggcag gccaaacccc attcggagca agaccaaaca gaacgcaatg
     6241 acgctgcccg ccgagcgttc gggtaggttg cttgagcata ccggcaacgg tatgcctaga
     6301 ggaatgactg tccgagacag aacccggctt accgcctgtc cggtgtgcat ccgacacgca
     6361 aatgccgtct gaaacctgaa atacggttcg gacggcattt ttatttaggc tgtgttttta
     6421 tattccgttg atgcagccct gatgagtcgg acaaaatgcc ttttactcaa taggaccggt
     6481 ttccgcccaa gcggaaataa acggctattt cccttgcaaa cagacggaat caggcgggta
     6541 tttaaatgac aatcggcagg ctgccttccg gttggggggg ggaaagcctg tcctgtcggg
     6601 tggaaacggt ttggtttcat tatgcggaaa aatgccgtct gaagggttca gacggtatcg
     6661 gcgcatcggg gcaggactta gtctgtgcct tcgagcattt tgttcaattt ggggacgagc
     6721 aggagcagca ggaagcctgt ggctgcgccg atgtagaaca gcagccggta gaagccgatt
     6781 tcgtcgcccg cctgatagcc tttttcaaac aatacgccgc ccaaagtgaa gcctaatgaa
     6841 aaggcaagga aattaagggc gaccatttgg gttttgaata aaggcggtgc gattttggtg
     6901 gagatggaca gcgcaatcgg ggaaatcatc agttcgccta tcgtgatggc gaggacgatc
     6961 agtgcgaaaa ccgcaatagg catcggcgtt ccggaggaaa taaagggaac gaatcccaaa
     7021 aacgacgcgc cggtaacaaa taccgccata gcgaatttca gcggggtttt gggttgtttg
     7081 cgccccattt ttgtccacat tgccgccatg agtccggaaa acaggatgac ccacaggctt
     7141 tgcatagaat ctttccaggc gacgggtaca gtaaacgaac cgatggtgcg gttgacggtt
     7201 tcgtcgaaat agacggttgc cacggtgtaa atctgaaacc agacggccca aaacatacag
     7261 atggtcagga aaagcgggat gtaggcgatg atgtgccgtt tgttgtcgga actgacgcgg
     7321 gggttggtca gcaggcgggc gaaataggcg atgacggcaa ggatgacggt agataagagg
     7381 atgccggaga aattgtcgag gttgacaagc ccggttttga tggcggttgc aagtgcggcg
     7441 atgagggtga tgccgacggc ggccgcagtt ttgccctgtc cttttgaaag cggatggggg
     7501 acggtggggt ggggcaggtt tttacgtccc aaggaataac gccacaagcc gaatgccata
     7561 ccgaccgccg ccgcgccgaa accataatgg aaaccgatgt tttcttgaag cagtccggtc
     7621 aataaggggc ctaagaagcc gccgatattg atggcgatgt agaaaatgga aaatcccgca
     7681 tcgcgcagcg ggcgcatttc gtcctgttcg tataatgcgc ccaccataga actggccgta
     7741 gatttcacgc cgccgctgcc caatgcgatg aatatcagcc cgattagaag gccgtacagg
     7801 cccggtgcgg cggcaaggac gatgtgcccg agcattacga cgatgcccga gaggaagagg
     7861 gttttttccg caccccatac gcggtcggca aaccacacgc ccaaaatggt ggacaggtac
     7921 acgctgccgc tgtatgcgcc gacaatgccg cctgcgaggg ttttgtctat gcccaagccg
     7981 cctttgtcgg cggtgtagta gaggtaaatc agcaggatgc cctgcattcc gtaaaatgaa
     8041 aaacgttccc acaattcgat atggaagagg gtggaaagct ggaaggggtg gccgaaaaat
     8101 gttttttctc cggtgcgggc gggatgccgg gacataaagg ttctcctaaa agattttctc
     8161 tcaataccgt ctgaaacgga tgcggacggc ggcggtgttt ttttgtatga aaatatcggt
     8221 ttttaaccga tattttcatt ctttgtcaaa cgacgcgctg ccgtttttcg cgggcggatg
     8281 tttttatatt tgtttcaatt caatggattg tattttagag gacgtgttcc gatacggcgc
     8341 gggtaaatcc ttttctgtca atggcttatc cgatagggcg gtttttactt agatggaaaa
     8401 tcacttcctt atatatccgc cacgccgaag ggcgcattat ggtggattaa ctttaaaccg
     8461 gtacggcgtt gccccgcccc ggctcaaagg gaacggttcc ctaaggcgcc caagcaccgg
     8521 gcgaaccggt tccgtaccat ttgtactgcc tgcggcccgc cgccttgtcc tgatttttgt
     8581 taatccgcta tatttttctt aaccatccct tccaatagcc gtgcgaaggt ttttttatat
     8641 cagtgggaaa tgcaatattc tattgttttt attgtagaat tttaaaaaca gattgttgtg
     8701 ttccgcgttt ttgccggttt ggaagcggtg gggcgcatca gccttttata aaggcatatc
     8761 ggaagcctgt ataaggtttt tgaacatatc gatcctgttc ccttgcaggc ggacggcagc
     8821 ggcggatggc gaaaggcggg ttttcaaatg tccggtttgg cggcgcgtct ttcagacggc
     8881 atggcggcgg agcgtgccgc gccgtctgaa aaccgtttat ttccctttgc cgtttccggc
     8941 ggagtctttt ttgtccgaac cgaagaggcg gtcgaaacgt atggtgtatg tcagctcgcc
     9001 gcccgacgaa cggctgccga tacgggcaac cgcctgtatg gcgcgggtca gccggtaaat
     9061 cagtttgacg gactgttccg cgctggagat gccgtattcg tagccgatgt agagtttgcc
     9121 ggtcagttgt ttgccgacgg tcagcacctg ttcggcgggg ttgagttcgc cggtttgcgc
     9181 gttgcggctg cgcttgctgg taaagcccaa atcatccacc agcccgatgc ggtcgttgat
     9241 ttgcccggca agcagcgcgc ctgcggctgc ggacagggcg gcattgtcgc cgctgctgcc
     9301 gctgccggcg cggttgagga tgagccagga gagcttgtct ttttcactca tcggttcgtt
     9361 tgccgtcagc gtaatgcgcg ggctgttgag gctgcccaat atttccacgc ccgcaccgac
     9421 gggggaaagg cggcgttcgg cgcggatgtt gaggttgggg tcgttgagcg ggccgacaaa
     9481 ggagactgtg cctttggtaa tgtctaaatc ctgcccgtat gctttgtaac gccctttgat
     9541 gacgcggacc gtgcccaccc cacgcacatt tccgcccggt tgcgcggtca gggtcagttt
     9601 gccgcctatg gtaacgtccg cgccgtagcc ggagaagcgg atgccgtcat tgaggtctaa
     9661 agtcaggttc atattgacgg ggagcgatgc cgccgcctct ttcttgactt cgcccaatac
     9721 gacgacatcg tcgccgacgg acggcatcga ggatttttgc gaaccgaaca gcccctgatc
     9781 agttttaatc ataccggtaa cggatatgcc tttttgcggc gaatagcgca ggcgggtgtt
     9841 gccggaaacc gtcaggcggc ggttggggcg ggacaggatg cggtatttgt cgaacaccgc
     9901 gccgatatcg acatcgggca cgctgttttc catgctgacc gtgccggaga gttccgccgt
     9961 cccttcgtgc cggaatttca ggctgtcgat tacccatttc ctgcctgcaa tatgcgaacg
    10021 cagcgagccg ttgtccaaga tgatgccttg ggtttggttg cggtaataga gcttgtcgcc
    10081 gttgatactg ccgcccaagt gcgggtcggc gatgctgccg ccgagggtta cggcggcatt
    10141 caggctgcct ttgacggttt gtccgaccgg taggaagttg cggaatactt cggcatcggc
    10201 aacggtcagg ttgagcctgc cgcccaaagg tgcggtatcg aaggagcggc tttgcccgac
    10261 ggtgatattg ccgttgattt tcccgtagtt gctgctaccg ttgacggcgg cattgacgga
    10321 cggagagctt acccgtccgc cgatttgcgc ggaggcattc aggctgccgg taatgttttg
    10381 cgcggcggcg ggcagaaagg gcttcaatgc gcccaagtcg ggaagggagg ctgtaatcct
    10441 gccgccgagc ggtgtatttg ccatattgcc gccgaaggcg ttgccgatgc ccaaatcggc
    10501 gttaatccgt ccgaaacgcg cgccgccgtc aagcaggatt ccgatgcggt cgttttgaaa
    10561 gcgcgttttc agggaaaatg cgttcaaacc caaagcctgc ccgccgggca atacggcatc
    10621 gccgctttgc cggctgatat tgaggtagcc gcgcgcgttg tgcccgtagg cgacatccca
    10681 gtcgccgttt aaaaccagat tgtgttcgaa gggcggtttg aagaaattgt gcaactcggc
    10741 gatgtgcagg ccgcgtgcgc cgccttttgc cgatatgccg gttttcctgt cccaagaaaa
    10801 gtgttgcagg ttgaggctgc cgcccattgc ctgccaattt gccgcacttg ccgccacgtg
    10861 ttccgcaccg gcttcgagcg tcatacggtt ttgcagcttg aggttgaatg cgccgccgat
    10921 gtcgaggatg ccgatgctgc ctttccatcg ggtaagttcc ctgttgatgc cgcctgaagc
    10981 gtccaaatcg agtttgaacg gtttgccgtc cagcgtcatg gcggcgtgtg tgcggatgcg
    11041 gtgctgcgcg cccgtacctt ccagcgtcag gccggcggta tcgacaaccg ccgcgccgcc
    11101 cgacagggaa aggcggccgc ccttgatatc ggcgcgcatc gggcggcttg tgtcgggcga
    11161 gcctttgagg gtaaaatcga gcgaacggat gtctgccgct ttgccgatgt gtaagttgcg
    11221 cgccgtgccg gaaaggtcgg tttcaaaggt tcggatgccg ccgtccaaat cgccggaaag
    11281 gtgtccgcgt acatttaaag accccgcgag tccgaaaccg aaacgggata aatcgggtgc
    11341 ggtgatattg aggttaagcc ggtcgccttt tttgccgaag ccgccgtctg ttttgacgat
    11401 gttccgcccc aaccgcaaat cgacggcggc acgcggaagg tggcgggact cgtaaacaat
    11461 gtcggcactg ccggcaatcg gcacgccgtt gaacgtaccg ggcaaaaaac gcattttgcc
    11521 cgtaaatttc tcttttgcca gttcaccggc aagatgaatc gaaccgttga tattgcctgc
    11581 cggaaattgc ggatcgatgc gtgaagggtc gaatgcgcgg gaacggatgt ccagcttgag
    11641 caggcggtct ttaaacagct cgagatagcc ttgcgcggtc aggctgcctt ccccggcgga
    11701 gatgttgacg gtgtcgaaca ccagtttccg ctgttcgttt gcggggtcgc ttgcgatggc
    11761 gaggctgccg tccgtgcgtg ccgtgccggt gccgagttgc caagagattt tgggcgaggc
    11821 ggtcgtgccg ccgatgccga tgctgccgtc caacctgcct ttgaacgcgg tttgcagcac
    11881 gtcttccgcg ccgacggagt tgatgcctat atttaaatca aggatgtctt tttcggtgtc
    11941 gattttgccc gacagcctga tgccgccccg tccgagcagg gcggcggacg tattgccgat
    12001 atgcaccgtg ccgtcctgcc ggatgacaaa gccgcccaaa acctgacgga cggggatgcc
    12061 gttgcggtcg gcaaagccgg ctttggtgtt ttccaaatcg agcgagcctt ccagcgcgat
    12121 gccgtctgaa aacgacggga tggcggtcag gtcgaaattc agcccggcat cgggcaggga
    12181 aggcacgaag gcggacggat tgatgttgaa tcctttgacc agtacttctt ccaatgtttt
    12241 atccaatgat tcggcaaacg ggtggatgac ggattttccc gagaggcgga tattgccgcc
    12301 gtcgatcgtc agttcggcgc gcacatcctt caggctgccg ctcagccgcg ccgtactgtg
    12361 tatggtttcg ccttcgaatc cgcctttggt gtaaatggcg gtatcgaggg caaacggttt
    12421 tttcaagccg accgaggctg accccgacga actgctccac ggcgtgtcgg cggccttcag
    12481 gtcgaggcgg tgccctttac ggtcgtaacg gtatgccgcg ttgaggcgtt cgagatagac
    12541 agtttgtttg tcaaaggttt tgcccatgct gattttgccc gtctcgaagc ggtcgagata
    12601 gacggtggcg ggcaggtcta tgctgtcggg caggccttga ggcgggcgtt cttctttagg
    12661 cggagtcggt ttggttacga tggcgatgtc gccggcggag atgtcggtga tgtgcaggct
    12721 gcggcgcatc agttcggacg gtttccacgc gaagcggaag cggctgattt taaggtctgc
    12781 cccctcggtt tctatcgacc agttgtcgcc gtcgaagccg tcgagcagtg tgcctttgag
    12841 gttttgggag gaaatgttta cgccgaacca ggacgggatt tggtacagcc cgaagcgcaa
    12901 acctgcttcc gtaccggcga tccagccgag gaaacatact gccaaaatca ggacagacag
    12961 cagtgccgcc gacagcttca gcagcgggcg gcgttttttt gccggcgggg cggggcggtg
    13021 ttcagacggc attttgcgcg tgccgttttc ggtcggatcg gtatctgtcg gtgcggtatc
    13081 ggtcataatg tctgtttcaa aatggtttgc tgcaatgccg tctgaagcgg ccgtatcggt
    13141 ttagaaacgc gttcccaagc tgatgtgcca gcggattttt ttgtcgctgt gtccgtaggc
    13201 gatgtcgaag gaaaacggcg cgagcgggct gaaccagcgc acgcccagtc ccgaaccgtg
    13261 tttcagcttc atacgtttga aattggcggc ggcatcgccc atatcgtgga ataccgcgcc
    13321 ggacagggtg cgcgtaaacg gcagttggta ttccaagctg cccaccaaga gggcgcgttc
    13381 gggcaggacc gatccgttcg gaccggcaag cccgatgctg tcaagttcgt aaccgcgcac
    13441 ggaagacgcg ccgccgctgc ggaacatcag ccccgagggg acatcggcat tgtcgcgtgc
    13501 aacggtgtaa cccgcttgtc cgcgtatgat gaacgtgccg agttttttgt tttcgggcgt
    13561 gaagaaataa cctgcgcggg cagaggtgcg gattagcgcg gtggaggaca ggaatgtgcc
    13621 caaagtcgtc ccgattttgc cgtcgaggta atggccgttt tcggggtgca gcacgttgtt
    13681 gagcagctgg cgtttccaag aggcggtcag catcgtggcg tggctgttgc ccaaatcgac
    13741 atccgagccg gggattttcc ggccttctgc gagaaattcc gcccccagcc tggcatcgat
    13801 gcccgcgcgg tcgcgcacat accagatgcc gccggagaag gcgcgttttt caaggttttg
    13861 ggtggtcgaa cggttgtagg aaacgttgct tgtccagtag ttgccctgat agttgcgcgg
    13921 ctggctgatg ccggcggcaa gcgtggtttc gtatttgtcc atatcccaga cgaccgagcc
    13981 gatatagcct ttgttgaaga ggttgtaata gtcgtaggcg attttgccgc ccaaaccgta
    14041 ttccgaatcg aggcggatgc cggtttcgag tttgtggcgt ttgacctcgg ttacgctgac
    14101 tttgacgggg acgcggtcgc cttggaggcg gtcgaagtcg gcttgtacgg acgcgccgga
    14161 ataatgcccg ttttgttcga gcgcctgttg gaagtcgagc agcaggtcga ggtcgtaggg
    14221 cgtgcccggt tggaagcgcg ccaggccgga gacggtttgt tcggggtaac gctgtgtgcc
    14281 ggtgatttca aagtcgccga aggcgatggg gcggccgctg tccacgacga cgttcaaatc
    14341 ggcggtggcg gtatcggggt tgacggccgc ccgggtgttg ccgagcttgg caagcgggta
    14401 gcctttgcgc gttaccgcgc cgaggacgga agttttgctg ttttcccaac tgtcctgatc
    14461 gaaatcgctg cctaccggct gctgccagtt ttccagcgcg ttgcggtagt attcggcgag
    14521 gttgccgtct gaaaggatgt cgccgaggat ggcgacgccg acgttggcga ttttggtgcg
    14581 cgggcccggt gtgatgtgca ccgtataagc tccgtctttt tccgtcaggc tgaccttgct
    14641 gctgaaatag cctttgctgc ggagcatggt tttgacgttg tccggtgctt cttcggcaag
    14701 gaatcccgtc tgttccttat ccaaaacctc ttcctgctgc tgcgtgatga gcggcaggtg
    14761 ttcttcgacc atatctttga tttcactgtc ctgcgtgtcg atgcggacgg ggaatttggg
    14821 ttttaatttg actgattcgg tgtcggggct tttgcttttg aacaatgcga aacccgccgc
    14881 cttgttttcg gaaaggtcgg cggcaggcgc gtatgcgtgc ggaaagaaaa ataaagccgg
    14941 caggagcagg gcggtcggtt tgatcatcat ggtacgggtg tcgtgcatcg gtgtggcgga
    15001 tttcgattct aacactgttc ggacggggcg ggacgcttca gacggcatac tgccgacggt
    15061 atttcggagg ttgcggcccg ccggaaatgt atctgcttgt tttaaggcat tttgtttcca
    15121 atccgcatat ggaaaacagg ctgcgtttcg gaatagtgtc gaaaaatagg caggattaaa
    15181 taaatttatt ctcgttcaat aaattaaacc gttgcgccga atgataaaac aaagcctgtc
    15241 cctgcatagg gcaaatcaac gcacctcaaa acacagcttg aaaaataaaa aaaacacaga
    15301 gaatgagttt tcttttaaaa tacaacattt tttaacatat cacaacataa ggaagaatta
    15361 tggcgttcgg caaatcctta tttcatgcga tcggccgcgt cagcctggtc aggcagattg
    15421 ccgccggttt ggcgttgggc atcgtaatcg gttcggtttc accgcaactg ggcttggcgg
    15481 caggcttgtt cggcagcctg tttgtcggcg cgctcaaagc ggtcgcgccg gttttggtat
    15541 ttattttggt ggcggccaca atcgcgcagc accaaaaagg caacaaggcg catatcaggc
    15601 cgattatcgt cctttacctc atcggcacgt tttccgcagc cctgaccgcc gtcatcgccg
    15661 gtatggtttt cccgacgcac attgttttgg cgggcgcggg cgatgtgtcc gccgcgccgc
    15721 cttccggcat tgtggaagtg ttgaaatcgc tgctgatgaa cctggtcgcc aacccgatta
    15781 acgcgattgc caatgccaac tatatcggca ttttggcttg ggctttggtt ttgggcgcgg
    15841 cgttgcggaa tcacggttcg gacgttacgc ggcaggtcgt tgccgatttg gcggaagcgg
    15901 tttccaccgt cgtgaaatgg attatccgtt ttgccccttt gggcattttc gggctggtgt
    15961 cgtccacaat cgcggagacg ggtttcggcg cgctggcggg ttatgcgaag ctgcttgcgg
    16021 tgctgttggg ctgtatggcg tttatcgcgc tggcggtcaa tcccgccatc gtgtggtgga
    16081 aaatccgccg caacccttat ccgctggtgt ttacctgcct gcgcgaaagc ggcgtgtatg
    16141 ccttctttac ccgttcttcc gccgccaata tccccgtgaa tatggctttg gcgaaaaagc
    16201 tggggctgca cgaagacact tattcgattt ccatcccgtt gggggcgacc gtcaatatgg
    16261 gcggcgcggc gattacgatt accgttttgg ctatggcggc ggcgcacacc caaggcatac
    16321 aggttgattt tgccaccgcg ctgctgttga gcctggtggc gacggtcagc gcgtgcggcg
    16381 cgtccggcgt ggccggcggt tcgctgctgc tgattccgct ggcgtgcagc ctgttcggca
    16441 tcagcaacga tgtcgccatg caggtggtcg ccgtcggctt catcatcggc gtgattcagg
    16501 attcggcgga aacggcgttg aattcttcaa ccgacgtttt gtttaccgct gccgccgatt
    16561 tgggccgaca gagaaaccgt gcggaataaa ccaagctatg ccgtctgaag cccgtttggc
    16621 gttcagacgg catattttta caattttatg ggcatatggc ggaaacaata ccggctgcct
    16681 tgaatatgtt tcaacacaca ggtcgataca taaagcgtcg ccctatgaag gagatgatga
    16741 tgtttaaacg cagtgtgatt gcaatggctt gtatttttcc cctttcagcc tgtgggggcg
    16801 gcggtggcgg atcgcccgat gtcaagtcgg cggacacgcc gtcaaaaccg gccgcccccg
    16861 ttgttgctga aaatgccggg gaaggggtgc tgccgaaaga aaagaaagat gaggaggcag
    16921 cgggcggtgc gccgcaagcc gatacgcagg acgcaaccgc cggagaaggc agccaagata
    16981 tggcggcagt ttcggcagaa aatacaggca atggcggtgc ggcaacaacg gacaacccca
    17041 aaaatgaaga cgcgggggcg caaaatgata tgccgcaaaa tgccgccgaa tccgcaaatc
    17101 aaacagggaa caaccaaccc gccggttctt cagattccgc ccccgcgtca aaccctgccc
    17161 ctgcgaatgg cggtagcgat tttggaagga cgaacgtggg caattctgtt gtgattgacg
    17221 gaccgtcgca aaatataacg ttgacccact gtaaaggcga tccttgtaat ggtgataatt
    17281 tattggatga agaagcaccg ccaaaatcag aatttgaatc gttaagtgat gaagagaaaa
    17341 ttaagaaata taaaaaagat ggagaaaagt ttactggttt ggttgctata aaagttgaaa
    17401 ataatgggtt gaataaatat accattattt atcaagctca gcctactcgt tctgcacggt
    17461 cgaggaggtc gcttccggcc gagattccgc tgattcccgt caatcaggcc gatacgctga
    17521 ttgtggatgg ggaagcggtc agcctgacgg ggcattccgg caatatcttc gcgcccgaag
    17581 ggaattaccg gtatctgact tacggggcgg aaaaattgcc cggcggatcg tatgccctcc
    17641 gtgtgcaagg cgaaccggca aaaggcgaaa tgcttgcggg cacggccgtg tacaacggcg
    17701 aagtgctgca tttccatatg gaaaacggcc gtccgtaccc gtccggaggc aggtttgccg
    17761 caaaagtcga tttcggcagc aaatctgtgg acggcattat cgacagcagc gatgatttgc
    17821 atatgggtac gcaaaaattc aaagccgcca tcgatggaaa cggctttaag gggacttgga
    17881 cggaaaatgg cggcggggat gtttccggaa ggttttacgg cccggccggc gaggaagtgg
    17941 cgggaaaata cagctatcgc ccgacagatg ctgaaaaggg cggattcggc gtgtttgccg
    18001 gcaaaaaaga tcgggattga tgttgatgcc gcccgaagcg caggtattca ttacggcggg
    18061 acgacgcgtt caaatcatca aacaagcccc cgaaaaaggc ggcatcccaa aatccggcac
    18121 tgaaattgcc gcgcgtatcg gcggcattcc gcagcctgcc ggggaaaccg tcggcagcag
    18181 ccggaatgtc gggtatttcc gcccgccttt ttcgggctgt tgaaatatga aaataaagca
    18241 taggccgtct gaatgttcag acggcctgtt tttttaacgg ctgtgtttca acactccgcc
    18301 ggttccaacc ggcagcagca gggagataat gccttgaacc ccgctcttcg gggcggcagg
    18361 gttcgggccg gtttggggaa aggcatcgcc tttccgaatc ggggcggtgc aacggggtgc
    18421 gccttatgtg ccgaaagggt ttgcggcaac ggtatgccgt ctgaagcggg tgcgttcaga
    18481 cggcattgtg atgtttgggg cgcgggtccg gttcaaatcc gcatcagttg ataaacccgt
    18541 tgtctttcat tttcttttca gaaaaatact ggccgcatac cagcagggcg gcgaggatga
    18601 tgacaaacag ccctgccata gtcagtgtga ttttgaagaa caattcggct tcgagaaaag
    18661 aaaaccaaag ttgcacaatc agcagcagca cccagccggc aaacagtccg agacaggcat
    18721 aaacgccgag cttaaacacg gtcccgctcc aaaatgacga cgtaggcttc gcgtttgaaa
    18781 aacagcagca tacggcgcaa atctttttcc atcgttacga cccgccagcc ttcgggggtt
    18841 tgtttgttga ggaacgcgct gaaattgacg gggttgacct ttgccgcgcc gaaaaacagg
    18901 ctggacaaca ggctttcctg ataaatgacg actttgtatt ctttcatcgt atttcctttt
    18961 cggttgaaac cccgcccttt ggggcggtag gatcagactt tatttgggat ggaacccctt
    19021 ccaaatcagg acgatacata gggcggtgct ttatgtatcg tcctgtgtgt tgaaacatag
    19081 gattccttta atcaagggat tgccttttca ggcaatccct taaattgcag cgatgcaatg
    19141 cggcgaaatt tattgcttat tgcccaactg cggcaatacc gagccttcgc ccagcgagag
    19201 caaaccgctt tgcgaataaa tacccagttt ggcgcgggtg tcggtgatgt cgaggtttcg
    19261 catcgtgagc tgtccgatgc ggtcgagcgg tgtgaacgcc gcaccttcga ctttttccat
    19321 actcaggcgt tcgggttggt aggtcaggtt gggcgattcg gtgttcagaa tcgagtagtc
    19381 gttgccgcgc cgcagttcga gggtaacttc gccggtaacg gctttggcga cccagcgttg
    19441 ggcggtttcg cgcaacatca aggcttggct gtcgaaccag cggccttggt agagcaaacg
    19501 tccgaggcgc aggccgttga tgcggtattg ttcgatggtg tcttcgttgt ggatgccggt
    19561 aaccaagcgt tcgtaggcga tgtggaacaa tgccatgccc ggggcttcgt agatgccgcg
    19621 cgatttggct tcgatgatgc ggttttcgat ttggtcgctc atacccaagc cgtggcggcc
    19681 gccgatgcgg ttggcttcga ggaagagttc gacggggtcg gcgtattctt tgccgttcaa
    19741 tgcaaccggc acgccttctt caaagcgcac gctgacttct tcgggttcaa tttcgacgtt
    19801 ttcgtcccag aaggcaacgc ccatgatggg cttaacgatt ttgatgcccg agttcaaaaa
    19861 ttccaagtct ttggcttcgt gggtcgcgcc gagcatattg gaatcggtgg agtaggcttt
    19921 ttcaaccgac attttgtagt tgaagccgtt ggcaatcaga aattcgctca tttcgtgacg
    19981 gccgccgagt tcgtcgataa attgctggtc gagccagggt ttgtagattt tcagcgcggg
    20041 attggtgagc aagccgtagc ggtagaaacg ctcgatgtcg ttgcctttgt aggtgctgcc
    20101 gtcgccccag atattcacat cgtcttcttt catggcggaa acgagcatgg tgccggtcac
    20161 ggcgcggccc agaggcgtgg tgttgaaata ggcgatgccg ccggtggaaa cgtggaacgc
    20221 gccgcattgg atggcggcga tgccttcgtg tgccagttgt gcgcggcagt cgatcaggcg
    20281 ggcgttttcc gcaccgtatt ccattgcttt tttgggaatg gcgttgtaat cgtcttcgtc
    20341 gggctggcca aggttggcag tgtaggcata aggcagcgcg cctttgagtt tcatccacaa
    20401 cagcgcggca gaggtatcaa gaccgccgga gaaggcgatg ccgactttct gaccgacggg
    20461 gaggttttgt aaaatagtat ggttttggtt catgaaaaaa tcctcgatag cttgaaaaat
    20521 caaagccatt atcttataaa tagaaaatat tgtcagccat catgccgtct gaacgggtat
    20581 tccgccgttt caggcggtat ttaaaaccga aaacattatg aacgcgttca acctcatcat
    20641 catcggagac gaaatcttac acggcagccg ccaagacaag catttcgcct tttttaaatc
    20701 cctgctcgaa tccaaagggc tgaagctcaa tcaggtgcaa tatctgcccg acgaacccga
    20761 tttgctggtc aggcaactgc gccgcagctt ttcagacggc ataccgacgt tcgttaccgg
    20821 cggcatcggc gccacgcccg acgaccgcac gcgccaaacc gcagcggctg ctttggattt
    20881 gcccgtcgtc cgccatcctg aagccgccaa gtttatcgaa ggcataaccc ggaaacgcgg
    20941 caagccgctc gattcgcccg aacacgccca acgcctgaaa atggcggatt tccccgaggg
    21001 cgcagagctt gtgcccaacc cgttcaacaa catcgccgga ttttccatcc gcgagcatta
    21061 tttcttcccc ggctttcctg tgatggcgca cccgatggcg gaatgggttt tggaaactta
    21121 ttatgccggc cgcttcaacc aaaccgaacg cggcagccgc agcgtgtatg tgtttgagca
    21181 gcccgaatcg cgcattacgc cgattataga gcatatcgag caaacttatc ccggcgtgcg
    21241 ttcgtacagc ctgcccagcg tcggttggac gcattcagac ggcacgcagg tcaaaccgca
    21301 cattgagttc ggcatcaaag ccgaaggaga agccgtcaac cttttggacg cggcgtgggc
    21361 ggaagtgttg cacagcttgg acggattggg agcagagctg aaaaatcggg taaactgaaa
    21421 actgtttgcc caaatcggac gatatttaag gaaaaccatg caataccgaa tcagaagaga
    21481 aaacgaagca cccgaagcaa aaaatgcggg ggaaacctta tgggagcgcg acattatgcg
    21541 cgaagtgctg ctgtccgcct atcgggacag gcgcagggag cggatgtgga aaaacatctg
    21601 gcgcgccgtc agcaccctga ttctggttgc cctgattgca ggcattttcc gaaaagacga
    21661 agccgcattg cagttggcgg gcaacacgcc gcataccgca gtcgtcaacc tgtacggcaa
    21721 aatcggcaac ggcgtagaag accaggtcaa aaaactcaaa gacgggatgg aggccgccta
    21781 caaaaacccg caggcaaaag ccatcgtcat ccgcgccaac agccccggcg gttcgcccgt
    21841 cgtctccaac accgcttttg aagaaatacg ccgtctgaag gcgcagcacc ccggcatccc
    21901 cgtttatctc gtggcagagg atatgtgcgc gtccggctgc tactacatcg cggcggcggc
    21961 ggacaaaatc tatgccgacc cgtccagcat cgtcggcagc atcggcgtta tcggcagcag
    22021 tttcgacgcg accggcctga tggaaaaaat cggcgtaaaa cgcagggtta aaattgcggg
    22081 cagcaacaaa ggcatgggcg atccgttttc gcccgaaacg cccgaacagt cgaaaatctg
    22141 ggaagaaatg ctgaccggca tacacggcga atttatcaaa gccgtcaaaa ccggacgcgg
    22201 cggccgcctc aaattccgac aatatcccga cgtgttcagc ggtcgcgtgt acacaggcgc
    22261 ggacgcgctg aaagtcgggc tggtggacgg actcggcaat atttacagcg tggcgcgaga
    22321 cgtggtcaaa gctcccgatg tggtggatta cactccgaag gacgatttcg gcagaatctt
    22381 gggcagacgc ttcggagcag aactcaaagc aagcgtttcg gaagctctgc aggccgtccg
    22441 gtagcgttgt ttacagtatt tctaagggaa taccagaata aaaatataca caaaaacact
    22501 cacctcccca gtatgctgcc actaagtcag ccgcgttact atttaccgtg cacttaaagc
    22561 cgcaaaaggc aggttgtccc cgggaggttt tgtgcatcct gataccgatc ggttgctgtt
    22621 gctgttgctg ttgctcaaag gacggaaggc cgccgataaa cgagattacc tgtttgtcgc
    22681 tattgacgat ttttatactc tgccattttg ccggacaaaa ccgcagacag tgctgccaag
    22741 tttctgaccg aacatctggc tgacccctgc ttgtacctgg ttgagtacgt ttactctgac
    22801 aatgataggt aatataaaga gccgtccaac atgctttcgg tgcagcttgt tatgataatg
    22861 ggattggttg gaggcttgcc cgatttgctt gtccgcagac caacggtaag gcggagcggg
    22921 ttatccgtac cttgatggag atgtggcatg aggaacagtc gtttgacagt ccggaacatc
    22981 agcaaaagga gttgtgctgc tttattaatt taggcgctgt cctagataac tagggaaatt
    23041 caaattaagt tagaattatc cctatgagaa aagccgtcta agccggtata aacaaaataa
    23101 actcattggg ctatttgtcg caggcgtaac tgcaagaaca gcggcagagt tggtaggcgt
    23161 taataaaaat accgcagcct atgattttca tcgtttacga tgactgattt atcaaaacgg
    23221 tccgcattta gaaatgtttg atggcgaagt agaagcagat gaaagttatt ttggcggaca
    23281 acgcaaaggt aaacgcggtc gcggtgctgc cggaaaagtc gcagtattca ggcttctcaa
    23341 acgaaatggc aaggtttcct ttgccgtccg ttatgtacgt ttcgtaacat tcgaagactt
    23401 cctgaaccct gcccgccgtt tcggtttctg tacccccctg ttagataagg gggggggaag
    23461 attcgaagcg gttgccggct tcctgccgtc cgccggcgcg tccgtcatca cgccggcaac
    23521 cgcctttgtc atcccttgct tatcttccat gtgcgaatcc tcaaaaacgg gcaaaaaaaa
    23581 agccctgtta cttgtagaaa gtaaagggcg ttaatttttg ttaatcgccc cttcttaggg
    23641 acgcaatata taaggtttat acggttactg tagcgaatac tcaaaccgct actttatttc
    23701 ctattatccg cgaacaagtg aaacctgaca gcattgttta tacggattgt tatcgtagct
    23761 atgatgtatt ggatgcgggc gaatt
//
LOCUS       DAXFWM010000026        23159 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000026 DAXFWM010000000
VERSION     DAXFWM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23159
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.026"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(300..1235)
                     /gene="hemC"
                     /locus_tag="ACFI8B_001004"
     CDS             complement(300..1235)
                     /gene="hemC"
                     /locus_tag="ACFI8B_001004"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC3834255.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1354..1869
                     /gene="ybeY"
                     /locus_tag="ACFI8B_001005"
     CDS             1354..1869
                     /gene="ybeY"
                     /locus_tag="ACFI8B_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC3834256.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1871..2695
                     /locus_tag="ACFI8B_001006"
     CDS             1871..2695
                     /locus_tag="ACFI8B_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC3834257.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2838..4217)
                     /gene="nhaC"
                     /locus_tag="ACFI8B_001007"
     CDS             complement(2838..4217)
                     /gene="nhaC"
                     /locus_tag="ACFI8B_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC3834258.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4507..5730)
                     /locus_tag="ACFI8B_001008"
     CDS             complement(4507..5730)
                     /locus_tag="ACFI8B_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC3834259.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6167..6580
                     /locus_tag="ACFI8B_001009"
     CDS             6167..6580
                     /locus_tag="ACFI8B_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC3834260.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6626..7255
                     /gene="nth"
                     /locus_tag="ACFI8B_001010"
     CDS             6626..7255
                     /gene="nth"
                     /locus_tag="ACFI8B_001010"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC3834261.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7393..8892
                     /locus_tag="ACFI8B_001011"
     CDS             7393..8892
                     /locus_tag="ACFI8B_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC3834262.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9095..10615
                     /locus_tag="ACFI8B_001012"
     CDS             9095..10615
                     /locus_tag="ACFI8B_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC3834263.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRADV"
     gene            10672..11757
                     /gene="nagZ"
                     /locus_tag="ACFI8B_001013"
     CDS             10672..11757
                     /gene="nagZ"
                     /locus_tag="ACFI8B_001013"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC3834264.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRTMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11838..11914
                     /locus_tag="ACFI8B_001014"
     tRNA            11838..11914
                     /locus_tag="ACFI8B_001014"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11872..11874,aa:Pro,seq:cgg)
     gene            complement(12250..12888)
                     /locus_tag="ACFI8B_001015"
     CDS             complement(12250..12888)
                     /locus_tag="ACFI8B_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC3834265.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CIETNGLNPVPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVGFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12885..13253)
                     /locus_tag="ACFI8B_001016"
     CDS             complement(12885..13253)
                     /locus_tag="ACFI8B_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC3834266.1"
                     /translation="MKFPSTLLVLLVAAEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QKQVQTLFGNQGLYNGFLGIGLVWSRFAVPDHAVYGATIPFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
     gene            complement(13519..13941)
                     /gene="queD"
                     /locus_tag="ACFI8B_001017"
     CDS             complement(13519..13941)
                     /gene="queD"
                     /locus_tag="ACFI8B_001017"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC3834267.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(14029..14544)
                     /locus_tag="ACFI8B_001018"
     CDS             complement(14029..14544)
                     /locus_tag="ACFI8B_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC3834268.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(14572..15231)
                     /gene="queC"
                     /locus_tag="ACFI8B_001019"
     CDS             complement(14572..15231)
                     /gene="queC"
                     /locus_tag="ACFI8B_001019"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC3834269.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVKQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIQTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(15416..15621)
                     /locus_tag="ACFI8B_001020"
                     /pseudo
     CDS             complement(15416..15621)
                     /locus_tag="ACFI8B_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            15720..16946
                     /locus_tag="ACFI8B_001021"
     CDS             15720..16946
                     /locus_tag="ACFI8B_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC3834270.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVPEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(16967..17248)
                     /locus_tag="ACFI8B_001022"
     CDS             complement(16967..17248)
                     /locus_tag="ACFI8B_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3834271.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            17327..17443
                     /locus_tag="ACFI8B_001023"
                     /pseudo
     CDS             17327..17443
                     /locus_tag="ACFI8B_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17444..19162)
                     /locus_tag="ACFI8B_001024"
                     /pseudo
     CDS             complement(17444..19162)
                     /locus_tag="ACFI8B_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(19264..19479)
                     /locus_tag="ACFI8B_001025"
     CDS             complement(19264..19479)
                     /locus_tag="ACFI8B_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834272.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(19493..19840)
                     /locus_tag="ACFI8B_001026"
     CDS             complement(19493..19840)
                     /locus_tag="ACFI8B_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834273.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(20026..21300)
                     /locus_tag="ACFI8B_001027"
     CDS             complement(20026..21300)
                     /locus_tag="ACFI8B_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC3834274.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQETMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLLLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(21361..21633)
                     /locus_tag="ACFI8B_001028"
     CDS             complement(21361..21633)
                     /locus_tag="ACFI8B_001028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834275.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(21708..21935)
                     /locus_tag="ACFI8B_001029"
     CDS             complement(21708..21935)
                     /locus_tag="ACFI8B_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834276.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(21944..23098)
                     /locus_tag="ACFI8B_001030"
     CDS             complement(21944..23098)
                     /locus_tag="ACFI8B_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC3834277.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
BASE COUNT         5713 a         6281 c         5735 g         5430 t
ORIGIN      
        1 ggtgtggaat ttagggataa tctagtacag ccacaataaa aaatacgaat atcatcattc
       61 tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct ttccgccgtc
      121 attcccgcgc aggcgggaat ctggaaaccc aatgctgcaa gaattgatta aaaacaactg
      181 aaaccgagca aactggattc ccgcctgcgc gggaatgacg aaataaggtg agtaaaactt
      241 attaaataaa ttaattaaaa acaataactt atattatttt ctttaaattt tcgataaaat
      301 caattttccg tattcaatac tgctccaatc aattcctgcg caccgtcgtc cgccagtttt
      361 ttggcaaccg cacgtccaag cgcatcggca tatccggcag gggcttgcgc gtccgcctgc
      421 aacacaatcg atccgtcggg atgcccgacc aatccgcgca agatcagcag cccgttttct
      481 tcagtgcaat acgcggccag cggcacttgg cagcttccgc ccaaagcgcg cgccaaagcg
      541 cgttcggcgg taacgcaggc gtgtgtggta tcgtggttta acggcttcaa gacttcgtac
      601 aaatcttcgc gatgcgtggc aatttcaata cccaatgcgc cttgtccggc ggcaggcagg
      661 ctgtcggatt ccgacaaaat catgcggatg cgttcatcca attccagacg ctgcaacccg
      721 gcggcagcca agataattgc atcgtattcg ccgttgtcga gtttggacaa acgggtttgt
      781 acattgccgc gcaggggttt aatcaccaaa tgcggatagc gcgcgcgcaa ctgggcttcg
      841 cggcgcaggc tggatgtgcc gacaaccgcg ccttcgggca tttcttccaa acgcgcgtat
      901 cggttggaca caaacgcgtc aaacggattg gcgcgttcgc tgatggcggc aagggcgaag
      961 ccttcgggca aatccatcgg cacgtcctta atcgaatgca ccgccaaatc ggcgcgcccg
     1021 tcttgaaggg attgttccaa ctctttgaca aacaagcctt tcccgccgac ttttgacaaa
     1081 gttctgtcca aaatccggtc gccgcgcgtg gtcatgccca aaatttcgac ttcgcaatcg
     1141 ggatacagcg ccttcagacg gccttggata tgtttcgcct gccacatggc aagcaggctt
     1201 tcgcggctgg cgataacaag ttttttcggg ttcatgttca atgcgttcaa agaatggaaa
     1261 cggcgtggag tctatcatat tttgaacgtt tcttcagacg gcaccgtcag gtctatgtta
     1321 tatttacgcc cacaaaacac gaaacccgcc acaatgaaac gcgccaaaaa ataccctttc
     1381 ttaacgttgc agcggcaacg tttccatttg aactttgaaa acgcctcttc cgccgccggc
     1441 atccccgccg aacgcgattt ttaccgctgg gcgtggtctg ccttgaaaaa cgaataccgc
     1501 cgcgccgaca tcggtttgat tcttttggac gaagaagaag cccgcgccta caaccgcgac
     1561 taccggggca aagattatgc caccaatgta ttgagtttcg cgctcaacga aggcgaaatc
     1621 ctgccctgcc aagtttcgga aagactgtac ggcgatttga ttatctgccc gcaagtcgtt
     1681 ttaaaagaag ccgccgaaca gggcaaaacg cctgaacggc attttgccca cttaaccata
     1741 cacggcacgc tgcacctgat gggatacgac cacatcaaag acgatgaggc cgaaataatg
     1801 gaagccgaag aaatccgcct gatgcgggcg gcaggctatc ccaaccccta ccgagaggac
     1861 ggacattaaa atggacggcg cacaaccgaa aacaaatttt tttgaacgcc tgattgcccg
     1921 actcgcccgc gaacccgatt ccgccgaaga cgtattaaac ctgcttcggc aggcgcacga
     1981 acaggaagtt tttgatgccg acacactgac ccggctggaa aaagtattgg actttgccga
     2041 gctggaagtg cgcgatgcga tgattacgcg cagccgcatg aacgtattga aagaaaacga
     2101 cagcatcgaa cgcatcaccg cctacgtcat cgataccgcc cattcgcgct tccccgtcat
     2161 cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc tcaaatatat
     2221 gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt tcgtgcccga
     2281 aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc atatggcaat
     2341 cgtcatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca tcatcgagca
     2401 aatcgtcggt gacatcgaag acgagtttga cgaagacgaa agcgccgaca acatccactc
     2461 cgtttccgcc gaacgctggc gcatccacgc ggctaccgaa atcgaagaca tcaacgcctt
     2521 tttcggtacg gaatacggca gcgaagaagc cgacaccatc ggcggcttgg tcattcagga
     2581 attgggacac ctgcccgtgc gcggcgaaaa agtccttatc ggcggtttgc agttcaccgt
     2641 cgcccgcgcc gacaaccgcc gcctgcacac gctgatggcg acccgcgtga agtaagcaga
     2701 gcctgcccgc accgccgttt ctgcacagtt taggatgacg gtacggtcgt tttctgtttc
     2761 aatccgcccc atccgccaaa cataaaaatg ccgtctgaaa ccggaaaaca ggttcagacg
     2821 gcatttcgct tatccgctta ttttttgctc aaagtcagcc ccgtccagcc gaataacagg
     2881 gttaaagcca aactcaaata gcagaaaaag gcataaggca gatattccca aacgggtacg
     2941 ccaagggcgt ggctgataaa tacgccgcac acgctccacg gcacgagcgg gttaatcacc
     3001 gtccccgcat cttccagagt ccgcgacagg ttgcgcgaat gcaggccgag tttgtcgtaa
     3061 acgggtttga acgtttctcc cgaaagcagg atgctcaaat attgctctcc aatcaggaaa
     3121 ttgaccccga ccgaagtcat ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag
     3181 gtacggacgg cctccagcag ggaaggaatc acaccgagcg caaacagcag cccgcccaaa
     3241 ctcataccga ggataacgat ggtctgcgta aagaacatac tctccaagcc gccgcgcgaa
     3301 atcagtttgg caatgtcttt aaacgcttcg ccttcgagtt tgtagccgcc ataaaaccac
     3361 gcgccgagct gacgcagatc gggcgtgctg tgcagatacg ttacggcaac ggcggcaatg
     3421 acggtaaaga gcatggcgac cacggcattg acgcgcatca atgccaaaac gaccaacagt
     3481 gcaaacggaa tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg gctgcggaag
     3541 gattcgacgc tgttcaaatc ctgcgcggcg acgctgggaa gaagccaaag catcagtgcc
     3601 gcgctgataa gccacgcggg gatggtggtg tacatcatgt ttttgatgtg ttcaaacagg
     3661 tcgataccga cgatggacgc ggaaatgccc gtggtgtcgg aaagcgggga cattttatcg
     3721 ccgaaaaaca caccggaaac aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc
     3781 gccgccatcc ccataaaggc aacgccgaca gtggcgcagg cggtcaggct gctgccgatg
     3841 gacacgccga tgacggaaca cagcgcgaag gcggaaaaat aaaaataagt cggggaaatc
     3901 agcccgaaac cgtaatacat cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc
     3961 atcagcccga tgaagaaaaa caggtaaacc gcgcccatac cctgattcaa cgcgcctatc
     4021 atccctgcct gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg
     4081 acgatggcgg caataatgga catatgcggc agccactcca atgaaatgat ggtatagccc
     4141 attgcggcaa tcagagcgac gacgacggca agtgcctcac cgcgcggcat atcgagtaag
     4201 gatttgaaag caaacatgat tctttgcccg atttgatata aagttaacgg attctaacac
     4261 accatgccga atctgcccga cgttttcaga cggcatttta gcgttttaca aaagccgggc
     4321 agaaacaaaa cggcaatttt ctcgatttaa attaattttt ttgaaatata atttttattt
     4381 ttttatttgt tggcatataa gacttttatg gattgaagta ttaaaacctc ggcacacacg
     4441 ccgcaaacac cggtacgcaa aaatgccgtc tgaaagatgt tcagacggca tttctcaaga
     4501 aaaagattat ttgtccgccc tatatccgta caccgcaaaa aacacgatat acaggtaaca
     4561 gataacggaa acgacaaacg aagacatcag ggtgtaagta tccgccgccc aaccttgaat
     4621 taccggcacg accgcaccgc cgacaatcgc ggtacacagc acaccggaag ccgcgttggt
     4681 gaatttgccc aaccctttgg ttgccaaaga gaaaatcgtc gggaacataa tcgagttgaa
     4741 aaaaccgatg gcaagcagcg accacatcgc cacatccgca ttgccgctgc ccgtcgccat
     4801 cgcgacggca agcagcacga ccgcagccga tgcgttaaac gccaaataac ggttgggcgc
     4861 gaatttcgcc attaccgccg aaccgaggaa acgtccgacc atcgctccgc cccaataaaa
     4921 cgacaggtaa tgcgcggcag aagcatgatc cagccctttc agataaccca acacattgac
     4981 catcagcgaa ccgatagaca cctccgcacc gacgtagcag aaaatgcctg ccgtaccgaa
     5041 cacgagatgt ttgtattgcc atacgccggt tttgccgtcg tggttgtgcg cgctttcctc
     5101 ggcggcaatt ttgcgcgcgt cgggcagccg gatcattttc acgaaaacgg caaggataat
     5161 cagcagcccc gccagtccca aatagggaat ctgtacggaa gaaatctgtt ccgccttgct
     5221 gacggtttgg gttgcgtccg ccaaaatcag gaacgcgccg atttgaggcg caatggtcgt
     5281 acccaaagcg ttaaacgcct gaaccagcgt cagcgttgcc gattccttgc cgggtttcgc
     5341 cagcagggta acataaggat taccggcgac ctgaagcagc gttacgccgg aagccaaaat
     5401 aaacaacgcg cccaaaaata ccgcgtaaga atggctgccc gcagcaggat aaaacagcag
     5461 gcacccgacc gccgtcagca gaaagccgcc gataacgccg tttttgtagc cgactttgcc
     5521 gacaaaagcc cccatcggga tggacattac cgcataggcg gtaaaaaagc aaaattgaat
     5581 cagcatcgcc tgaacgtaag agaggtcgaa aatttctttc aaatgaggga taaggatgtc
     5641 gttcatgcag gtaataaaac ccatcatgaa gaacagcgtg gtcaagacga ccaatgggga
     5701 cgtatggttg ttttgtgatt gcgcagacat gatttgcctt tcgtggtgtc ggaatattgc
     5761 ccgtttcaag aaaatagaaa ttgacatttc ttaacggcaa tgcaaatcat actgatattt
     5821 gaacattttg caaactttat cggatgcagg caatgcgccg atctgaaatt caagcagcag
     5881 aaattaatat tttacagttg tttgcatagg ttttaataga ttttaaaaaa ttccggcggg
     5941 cttgcaaaaa ccgttcccaa taaaatgaaa atcctccgcc gtatccggaa aattcggaaa
     6001 tgccgcatcg gggcatcaaa accggagcgg aaagaatgtc gtatgcccga caaataaagg
     6061 ggcatacggc tgtgccgtat ttttgcaccg tcctccgccc ttcggcgggg gcgtgaaaat
     6121 accgtataat ccgtcgccga tttttacctg tttgggactg tttgcaatga taccctccta
     6181 cacctacgcc gccctcgctt tttccgcctt cacttccgcc accctcctgc ccggcacgtc
     6241 cgaagccgcc ttcgccctgt tcgtccgcaa cttccccaaa cacgcatacg gcgcgttgct
     6301 gtgcgccggc cttgccaacg gattgggcag tatggtttcc tattggatgg gacgtttgct
     6361 gccttcccga aaaatgccgt ctgaaaaaac actgaatctg atacggcgtt tcggtatttg
     6421 gctgcttgcg tttgcgtggc tgcccgtcgt cggcgacgca ctgccgctta ccgccggctg
     6481 gctgcggttg aatccgtgga caagcgggct gatgctggtt atcggcaaaa cggcgcgtta
     6541 cgccttcatt ctgtggggaa tgcaatatta cgccgcctga accgtgcgtt ataatgccga
     6601 ccgcacaaac cgactgcaca aaaaaatgaa cagacaaatc cgccaagaaa tcttcgagcg
     6661 tttccgcgcc gccaaccccc atccgaccac cgagctgaat ttcaactccc ctttcgagct
     6721 tttaatcgcc gttctgcttt cggcgcaggc gaccgatgtc ggcgtaaaca aggcgacggc
     6781 gaagctgttt ccggttgccg atacgccgca ggcgatgctg gatttgggct tggacggcgt
     6841 gatggaatac acgaaaacca tcgggctgta taaaaccaag tccaaacaca ttatgcaaac
     6901 ctgccgcatc ctgctggaaa aatacaacgg cgaagtgccg gaagaccgcg aggctttgga
     6961 atcgttgcct ggcgtggggc gcaaaacggc aaacgtggta ttgaacacag cgttcggaca
     7021 ccccgttatg gcggtcgata cgcatatttt ccgcgtgtcc aaccgaacca aaatcgcacc
     7081 aggaaaagat gtacgcgaag tcgaagacaa actgatgcgc ttcattccta aagaatttct
     7141 gatggacgcg caccactggc tgattttgca cggacgctac acctgcaagg ctttaaagcc
     7201 gcaatgccaa acctgcatca tcaacgattt gtgcgaatat cccgccaaat cctgaccgcc
     7261 gccgcccttc aaaatgaaac gcttgaaaat tcggacggac gttgtagaat ttcaacctat
     7321 gccgtctgaa gccgacaaaa tgcgcttcag acggcattaa agccggtacc ggcatccgac
     7381 aaaggacaaa acgtgttcaa aaaataccaa tacttcgctt tggcggcact gtgtgccgcc
     7441 ttgctggcag gctgcgaaaa ggcaggcagc tttttcggtg cggacaaaaa agaagcatcc
     7501 ttcgtagaac gcatcgaaca caccaaagac gacggcagtg tcagtatgct gctgcccgac
     7561 tttgcccaac tggttcaaag cgaaggcccg gcagtcgtca atattcaggc agcccccgcc
     7621 ccgcgcaccc aaaacggcag cggcaatgcc gaaaccgatt ccgacccgct tgccgacagc
     7681 gacccgttct acgaattttt caaacgcctc gtcccgaaca tgcccgaaat cccccaagaa
     7741 gaagcagatg acggcggatt gaacttcggt tcgggcttca tcatcagcaa aaacggctac
     7801 atcctgacca atacccacgt cgttgccggt atgggcagta tcaaagtcct gctcaacgac
     7861 aagcgcgaat ataccgccaa actcatcggt tcggatgtcc aatccgatgt cgcccttctg
     7921 aaaatcgacg caacggaaga gctacccgtc gtcaaaatcg gcaatcccaa aaatttgaaa
     7981 ccgggcgaat gggtcgctgc catcggcgcg cccttcggct ttgacaacag cgtgaccgcc
     8041 ggcatcgtgt ccgccaaagg cagaagcctg cccaacgaaa gctacacacc cttcatccaa
     8101 accgacgttg ccatcaatcc gggcaattcc ggcggcccgc tgttcaactt aaaaggacag
     8161 gtcgtcggca tcaattcgca aatatacagc cgcagcggcg gattcatggg catctccttt
     8221 gccatcccga ttgacgttgc catgaatgtc gccgaacagc tgaaaaacac cggcaaagtc
     8281 caacgcggac aactgggcgt gattattcag gaagtatcct acggtttggc acagtcgttc
     8341 ggtctggata aagccagcgg cgcattgatt gccaaaatcc ttcccggcag ccccgcagaa
     8401 cgtgccggcc tgcaggcggg cgacatcgtc ctcagcctcg acggcggaga aatacgttct
     8461 tccggcgacc ttcccgtcat ggtcggcgcc attacgccgg gaaaagaagt cagcctcggc
     8521 gtatggcgca aaggcgaaga aatcacaatc aaagccaagc tgggcaacgc cgccgagcat
     8581 accggcgcat catccaaaac agatgaagcc ccctacaccg aacagcaatc cggtacgttc
     8641 tcggtcgaat ccgcaggcat tacccttcag acacataccg acagcagcgg caaacacctc
     8701 gtcgtcgtac gggtttccga cgcggcagaa cgcgcaggct taaggcgcgg cgacgaaatc
     8761 ctcgccgtcg ggcaagtccc cgtcaatgac gaagccggtt tccgcaaagc tatggacaag
     8821 gcaggcaaaa acgtccccct gctggtcatg cgccgtggca acacgctgtt catcgcatta
     8881 aacctgcaat aacgcacata atttaaccaa aatattttta aatgataaaa tgcccgtcaa
     8941 taccgattaa cgggcatttt acaaaaatca gttggttgat tttaaacaaa tattttttaa
     9001 gacaaaaaat atttccaccc attttgccaa caaaacccaa accggaggcg gacagatacc
     9061 cacccatccg gacgcttaac accccccata aaccatgcag ctgattgact attcacattc
     9121 atttttctcg gttgtgccac cctttttggc actggcactt gccgtcatta cccgccgcgt
     9181 actgctgtct ttaggcatcg gtattttggt cggcgttgcc tttttggtcg gcggcaaccc
     9241 cgtcgacggt ctgacacacc tgaaagacat ggtcgtcggc ttggcttggg cagacggcga
     9301 ttggtcgctg ggcaaaccaa aaatcttggt tttcctgata cttttgggca ttttcacttc
     9361 actgctgacc tactccggca gcaatcaggc gtttgccgac tgggcaaaac ggcacattaa
     9421 aaaccggcgc ggcgcgaaaa tgctgaccgc ctgcctcgtg ttcgtaacct ttatcgacga
     9481 ctatttccac agcctcgccg tcggtgcgat tgcccgcccc gttaccgaca agtttaaagt
     9541 ttcccgcgcc aaactcgcct acatcctcga ctccactgcc tcgcccatgt gcgtgctgat
     9601 gcccgtttca agctggggcg cgtcgattat cgccacgctt gccggattgc tcgttaccta
     9661 caaaattacc gaatacacgc cgatggggac gtttgtcgcc atgagcctga tgaactatta
     9721 cgcgctgttt gccctgatta tggtattcgt cgtcgcatgg ttctccttcg acatcggctc
     9781 gatggcgcgt ttcgaacagg ctgcgttgaa cgaagcccac gacgaaaccg ccgcttcaga
     9841 cgctaccaaa ggtcgtgttt acgcattgat tattcccgtt ttggccttaa tcgcctcaac
     9901 ggtttccgcc atgatctaca ccggcgcgca ggcaagcgaa accttcagca ttttgggggc
     9961 atttgaaaac acggacgtaa acacttcgct ggtattcggc ggcacttgcg gcgtgcttgc
    10021 cgtcgtcctc tgcacgttcg gcacgattaa aaccgccgat tatcccaaag ccgtgtggca
    10081 gggtgcgaaa tccatgttcg gcgcaatcgc cattttaatc cttgcctggc tcatcagtac
    10141 ggttgtcggc gaaatgcaca cgggcgacta cctctccacg ctggttgcgg gcaacatcca
    10201 tcccggcttc ctgcccgtca tcctcttcct gctcgccagc gtgatggcgt ttgccacagg
    10261 cacaagctgg gggacgttcg gcattatgct gccgattgcc gccgccatgg cggtcaaagt
    10321 cgaacccgcg ctgattatcc cgtgtatgtc cgcagtaatg gcgggggcgg tatgcggcga
    10381 ccactgttcg cccatctccg acacgaccat cctgtcgtcc accggcgcgc gctgcaacca
    10441 catcgaccac gttacctcgc aactgcctta tgccctgacg gttgccgccg ccgccgcatc
    10501 gggctacctc gcattgggtc tgacaaaatc cgcgctgttg ggctttggca cgaccggtat
    10561 tgtattggcg gtgctgattt ttctgttgaa agataaaaaa cgcgccgacg tttgaccttc
    10621 ccccctgttc agacggcata tgccgtctga agttttttgg agcaaaccgc aatgaccgtc
    10681 ccccatattc cgcgcggccc cgtcatggca gacattgccg ccttccgcct gaccgaagag
    10741 gaaaagcaac gcctgctcga tcccgcaatc ggcggcatca tcctcttccg ccgcaacttc
    10801 caaaacatcg aacaactcaa aacactcacc gccgaaatca aagccctgcg cacacccgaa
    10861 ctcattatcg ccgtcgatca cgaaggcggc agggtgcaac ggtttatcga aggttttacc
    10921 cgcctgcccg caatgaacgt tttagggcaa atttgggaca aagacggcgc gtccgccgcc
    10981 gaaaccgctg ccggacaagt cggccgggtt ttggcaaccg agctttccgc ctgcggcatc
    11041 gatttgtcct tcacgcccgt cttggatttg gactggggaa actgcgccgt catcggcaac
    11101 cgcagcttcc accgcaatcc cgaagcggtc gcccgcctcg ccctcgccct tcaaaaaggt
    11161 ttggcaaaag gcggtatgaa atcatgcggc aaacatttcc ccggacacgg atttgtcgaa
    11221 ggggacagcc atttggtgct acccgaagac gggcgcagcc tcgacgaact cgaagccgcc
    11281 gacctcgccc ccttccgcac tatgagccgc gaaggtatgg cggcggtcat gcccgcccac
    11341 gtcgtttatc cgcaagtgga cacaaagccc gcagggtttt ccgaaatctg gctcaaacaa
    11401 attttgcgcc gcgatatcgg attcaaaggc gtgatttttt ctgacgattt gaccatggag
    11461 ggcgcgtgcg gggcaggcgg catcaaagaa cgcgcccgca tttcctttga ggcaggttgc
    11521 gacatcgtcc tcgtctgcaa ccgtcccgac ttagtcgatg aattgcgcga cggtttcacc
    11581 attcccgaca accaggattt ggccggacgt tggcaataca tggaaaactc gctcggtcat
    11641 gaggcggtac aagccgttat acagacaacg ggtttccaag cggcgcaagc ctttgttgca
    11701 ggccttgcct cgccgcagga cacggcgggc ggcgtaaaag tcggagaggc cttttaaatc
    11761 cgcccgacac gcggcaaaaa ggcaaaacgg tataggcacg ccgtattttt tgcgctataa
    11821 tcacacctta attaattcgg aatgtagcgc agcccggtag cgcacttcgt tcgggacgaa
    11881 ggggtcggag gttcgaatcc tctcattccg accatattca gaccgcctcg acatcgattg
    11941 ccggggcggt ttttcccttt tggcggcaac aaaaaaatgc cgtctgaaac agaatgctgt
    12001 ttcagacggc atttcgtttt ccccaatcct gccatttgcg cgcactgaca aagggtatcg
    12061 gcaaggttca aaagaccatc ggaatctagg aagcctggcg tgtacccaac cgcggcatct
    12121 tattcattaa ggcttcgtcc tgattgggcg gcggaaatgt cggcagtttc acgcggaagc
    12181 ggcacacttt ccccgtatgt caaacatcgg caaaacggta cgccggccct ttgagttaca
    12241 ctgcttaaac tactctattc ccgcccattt gtgcgtctgc acgctcaact gccaatgcac
    12301 gggcgcgtcg gggcggctgt tcagaatacc gatttggcgg atggtgtcgt aaatgttcat
    12361 tgcgccgtct tgttcgcagg gcgaaaggta gtaatgatgc gcgcggattt tacgttccat
    12421 attttcgcag aatccgacga catcgccgtc ggcaacaatc cgcacttcgt cggctgtttc
    12481 gatacagctt ttttcgtatt tggcggcata acaggctttg gggctggtgg cgacatagtc
    12541 gatttgcggc ggcacgggat tgagtccgtt ggtttcgata cagaggaaat agccttccgc
    12601 cttgagcgtg tccagcagca tatcgagatg cggctggatg gtcggctctc cgccggtgat
    12661 gatgatgttg cgggcggcat aagttttcag acagcctaag atgtcggaca ggctcatcat
    12721 accaaatgtc aaataatcgg tatcacacca gccgcaggcg aggttgcatt tgcccaaacg
    12781 gacgaaaacg gcaggcatac ccgtgtttcg gccctcacct tgcaggcttt caaagatttc
    12841 gacgatgcgg tactggggat tttcgggggc gacgctgatt tttttcatac cgccaacacc
    12901 gccgccgcca aaaatgcggg caaaccttgt ttgacgagta tgcctttgtt gccggaagag
    12961 aacgcgcccc acgcggcggc aatcaatacg aaaccgagaa acggaatcgt tgcgccgtac
    13021 acggcatgat ccggcacggc aaaccgcgac cacaccagcc cgatgccgag aaagccgtta
    13081 tacagccctt ggttgccgaa cagggtctgc acttgctttt gttccataaa ttcataaggc
    13141 agcttgaacg tttccgccgc tttttcgctg ggaatctgcg tcatttccag ccaggcgatg
    13201 tagaaatgtt cggcggcgac gaggaggact aagagggtgg aggggaattt catgggtgtt
    13261 ccttttaatg ggtgtgtcgg tatggaatgc cgtctgaaga acggagaatc cgcacaggag
    13321 aaaaaccgct ttgcggtaaa ttcaaataag gacgctccac actgtcattc ccgcgcaggc
    13381 gggaatccag agatttggaa ttgctgcaat ttttaaatat tcctgataaa tcaaggtctg
    13441 gattcccgcc tgcgcgggaa tgacgcggtt gggcatgtct tatttgaatt tactatatcg
    13501 atacgttcaa gattttccct actccccttc atactccgcg cacgatgtcg gcgtttccca
    13561 caatttgacg cggcacacgt tcagccccgc gtttttcaga cggcagtaca tttcgaccgc
    13621 catattttcg gcagttgtgc ggcagggcag gcgcagggtt ttcatattcc agccttccaa
    13681 aagcgcggcg atttgagatt cgcgcccgtt gccgccgtgg tagatgaagg cgtggtcgaa
    13741 atggtcggta atgtgttgtt tcacaatggc tttcaagtcg gtaaagtcca tcaccatacc
    13801 gtctttcgct ccgcctttga tggtgccgtc tgaaacggtg atttcgagtt tgtaggtatg
    13861 cccgtgcagg ttttggcatt tgccgtcatg cccgtcgagc atatgcgagg agtcgaaggt
    13921 aaatatcttg gtaattttca tcccgattcc ttttcagtga aaaccccgcc gctttccgcc
    13981 cggagggtgg cttgatgtgt cgttttgcgt ggaaacatat ccgctgcttc aaatgggcaa
    14041 atagccttga aagccgattt cggcgggttt gccgtgcgga ttcggggtca ggcggatatg
    14101 gaaattgccg atgtgctcga gcgcaagcag gttgtccacg tctttcaagt tgtctttatg
    14161 ctgccgaaca tagtcggtca acagggtttg cagggctttt tgtttggctt cggcaggcgt
    14221 ggcggcggcg aacagcccga acgcgtgcgc ctcggcaagc tgccccgcgc gatagccgcc
    14281 gacattggca aaatacaggc gcggagcgtt ttcaggcggc attttcgttt tgggcgtttc
    14341 ggatacggcg atgtcgtaac cgtccgccca ttcgacaacc tgccaaccgt cgatgtggat
    14401 tttgtccgta tcgccgaacc acgcggcttt gagtgcgggg acggcttcgc ggtagtcgtc
    14461 gcacacggca aattggatgt cgtgcacttc gatattcgac cggccggcat tgccgccgag
    14521 gtaaaacata tggagcttgg gcatatcgta ttccttgtgc tttgcgcgcg ttcagacggc
    14581 ctttttactt tccagacatt ccgccagccc gcgttcgcgc aagatacagc tcgggcattc
    14641 gcggcagccg ccgacgatac ccttgtagca ggtgtgggtc tgttcgcgga tgtagtccaa
    14701 cacgcccatt tcgtccgcca acgcccacgt ttgcgccttg gtcaggtaca tcagcggcgt
    14761 ctggatttga aaatcgtagt ccatcgccag attaagggta acgttcatgg atttgacgaa
    14821 cacgccgcgg cagtcgggat agcccgaaaa atcggtttcg cacacgcccg cgatgatgtg
    14881 ccggataccc tgccctttgg caaaaatggc ggcgtaaagc aggaaaagcg cgttacgccc
    14941 gtccacaaag gtattgggaa cgccgttgtc ggcggtttcg atggcggcgg tttcgtccat
    15001 cagggcgttg tgcgtaatct gccgcatcag gctcaagtcg agtacggttt gtttgacgcc
    15061 caaatcctgc gcgatccact ctgcgcgttc cagctcgacg gcatggcgtt gcccgtatcg
    15121 gaaggtaatg gcttggacgt tttcgcgccc gtaggtttgg attgcctgaa tcaggcaggt
    15181 ggtcgaatcc tgaccgcccg agaagatgac caaggctttt tggtttgaca tatcgaatcc
    15241 tagttttggt aacgcgggag gtttgcgaac cgcgaagttg gacagtaaag acgcggatta
    15301 tacaggcaaa cgcgttcaga cggcatctga acgccatgcc gtctgaacac gcggcgtatg
    15361 ccgacttgac ggcgagttcc gccaagcgtc tgccttgtgc gaaggcgata ttgttttctt
    15421 cggcggtcag gacgggagcc gctgtcggga atatctttat ttgacggctt cgcagacggc
    15481 ggacactttg gggacggtgc gcaatacggc ttcgcaacct tcaacgctgt cgatgccgcg
    15541 agtgattcgg cgtgcgggat tgcgggtgct gccgttttgg gaatagtaga ggacgaggat
    15601 tttgagggga tttgggttca tgggcggatt ccgttaaaat agtttggtgt tcagacggca
    15661 taacggttgt ccagatgccg tctgaaacag tttgaaaaca tgaaacctgg taagagatta
    15721 tgaccttttt acaacgttgg caaggtttgg cggacaataa aatctgtgca tttgcatggt
    15781 tcgtcatccg ccgtttcagt gaagagcgcg taccgcaggc agcggcgagc atgacgttta
    15841 cgacactgct ggcactcgtc cccgtactga ccgtaatggt cgcggtcgct tcgattttcc
    15901 ccgtgttcga ccgctggtcg gattcgttcg tctccttcgt caaccaaacc attgtgccgc
    15961 agggcgcgga tatggtgttc gactatatcg acgcattccg cgatcaggca aaccggctga
    16021 ccgccatcgg cagcgtgatg ctggtcgtaa cctcgctgat gctgattcgg acgatagaca
    16081 atgcgttcaa ccgcatctgg cgggttaaca cgcaacgccc ctggatgatg cagttcctcg
    16141 tttattgggc gttgctgact ttcgggcctt tgtctttggg tgtgggcatt tcctttatgg
    16201 tcgggtcggt tcaagactcc gtactctcct ccggagcgca acaatgggcg gacgcgttga
    16261 agacggcggc aaggctggct ttcatgacgc ttttgctgtg ggggctgtac cgcttcgtgc
    16321 ccaaccgctt cgtgcccgcc cggcaggcgt ttgtcggagc tttgattacg gcattctgcc
    16381 tggagacggc acgtttcctg ttcacctggt atatgggcaa tttcgacggc taccgctcga
    16441 tttacggcgc atttgccgcc gtgccgtttt tcctgctgtg gttaaacctg ctgtggacgc
    16501 tggtcttggg cggggcggtg ctgacttcgt cgctgtctta ttggcagggc gaggccttcc
    16561 gcaggggatt cgactcgcgc ggacggttcg acgacgtgtt gaaaatcctg ctgcttctgg
    16621 atgcggcgca aaaagaaggc aaagccctgc ccgtacccga gttcagacgg catatcaaca
    16681 tgggctacga cgagttgggc gagcttttgg aaaagctggc gcggcacggc tacatctatt
    16741 ccggcagaca gggctgggtt ttgaaaacgg gggcggattc gattgagttg agcgaactct
    16801 tcaagctctt cgtgtaccgc ccgttgcctg tggaaaggga tcatgtgaac caagctgtcg
    16861 atgcggtaat gacgccgtgt ttgcagactt tgaacatgac gctggcggag tttgacgctc
    16921 aggcgaaaaa acagcagcag tcttgagtat ttttgaaact gtatttttat cctaaagaca
    16981 ttttcctatc tgctgattcc accgtttcct ttctgtgtgc cgccataatt tttataatag
    17041 acaagccatt caagtttgca ttgactgctt tttcattggc aatatccaaa tgactggccg
    17101 cttcatctaa aaataggatt ttcggttcgc aatataaggc ccgcgccaat acgatacgtt
    17161 gtttttgtcc gcctgacagt gcgctgccca tatcgccgat caaggtttca tagcccatag
    17221 gcatggcgga gatttcttcg cgaatcatag caaggcaggc gcaatgttcg atttttcctc
    17281 tatccggttc tgtatcgaaa aaacagatat tctctgcaat tgagccagca aatgcaaata
    17341 tagtggatta aatttaaatc aggacaaggc ggcgaagccg cagacagtac agatagtacg
    17401 gcaaggcgag gcaacgccgt accggtttaa atttaatcca ctataaaaca tcgtcctgcc
    17461 tgaccatcgc actcaaattg cggataaaac gtggcggtaa gctgtaaatg tcgtgcccat
    17521 ttatcatgac tttgcctgat tcgggaggta gattgccgct taaaatatcc aaaaatgtcg
    17581 atttaccgca gcccgatcgt ccgattaacg caagcgattc accacgtccg atcttgaagc
    17641 tgacgttttg taaaacatat ggctcgttat cggcataacg gaaagaaaca ttttccactg
    17701 agatttcgat gtcgtctgaa atatcaggaa tgcctacatc cggcgtagct tccgattctg
    17761 tttcattcaa tacggtgtca gctaaccgtt ccgcatgaag tgacagcatt ttaatttgca
    17821 caaactgatc gaccagcgag ccaatacgtc cttcaaactg tcccttataa gccaaaaaag
    17881 ccattagtac gccgacggta aagacgccgt ccaataccat gcccgcagct aagtaaataa
    17941 tcaaaatatt tgccaaaccg aaaagcagct tgtttgccaa gccgaaccaa attcccagtt
    18001 tctgcaccgt cagtcccgta ttaaccgtat ccaccagcag ccccatccat gcagaatatc
    18061 gttgaggacc tttaccaaat tgcttgactg ctcggatacc gcgtaccgtt tccataaaat
    18121 agctgctttg tttggcagca tggatgatgt tttcctccgt cgcccgtcgc aacggataat
    18181 aggctaccca acggactgcg atataaacta ccagtgtcgc tgcaaccaaa cctgtcagta
    18241 tcgggctgta cactatcatt agcacaaaag taaaaacagc cgttaggctg tttaaaacca
    18301 acacgaaaaa cgttgaagtc agagttccct gaatactatc caccgccccg aagcgcgata
    18361 ccacatcgcc caaatgccgt ttagcgaaat aatctgtagg caaatctatc aaacgtttga
    18421 aaacgtttga tttccactga acatttaacg tcgttgaaaa atgcatgccc aaccagccct
    18481 gcatgacaga caccgtattt tgtaccagaa gtagcaaggt aaagcccaaa gccagcgtcg
    18541 ctaacagatt acgatcgccc gttaccacta catggtcgat gacccattgc ataaaaaacg
    18601 gcgacaccaa agcaaacact tccattgctg ctgccaaagc caaaagctga aacaatgtcc
    18661 gccccagccc agaaatcccg cgcaacatgg gtaggatgcg gatttcctgc ttttcttccc
    18721 ctgcctcgaa acgcgtgttt ggccacagtt ccaaagcaat tcccgtaaac ttgcgcgata
    18781 tttcctccgt cttgactttg cgtcgtcccg aagccggatc catgacggca gccccgtccg
    18841 aagataccga ttccagcacc acaaaatgat tcaaatccca atgtagaata cagggcaggc
    18901 gcaaactgcc caattcgtct aaatccagcc tcaacgcccg tcccgtcagc cccatatcat
    18961 cagcaaaacg aacaatatct gccaaattct cacccttaag tgacagacag tattttgaac
    19021 gcagtgcgcg caaatccgta tagaaaccat aaaatccggc cacagccgct agacatgcca
    19081 agccacactc cgctacttct gtttgcagta taacgggcag cttttttgtc aagcccaaag
    19141 acaggttttg cagataatcc atagatttcc ttgttgaatc agaaaagaga gatgggaaga
    19201 cgggaatggt ttggaaaggc cgtctgaaaa ggtttcagac ggcctgcagc cccatattgg
    19261 ctttcaatgc gaccggtttt cctcgcgttt caaatacagc agatacgcaa gaaaagacaa
    19321 agcccctaca ggtcctgcga cggtaaccga aaaaaacctg cctttgtaaa acacaccgtc
    19381 gaagtttaaa aaatagcaaa ggatgtcggt caggatgctg atggcaagat aaatcagcac
    19441 agcggctttc cagtgtttca ggtaatgtgc gaaatccatt tcaaactccc gatcaacagt
    19501 tgcacgggtc gcaatggcag tcttctccgc attggcagtt gcgcctgtcg atcatttttc
    19561 cccattttgc accggcaaaa ccgccgatat gcccacccaa tgcagaaccg ccctcagctc
    19621 ctacccatcc gccgcctgcg ccgccgatgg caattccggc aggatttccg cctgttacca
    19681 atgcgccgcc cgccattccc gtgaatgctc cggcaaacga ccctaaagcg ccgccgacct
    19741 tgcttccgac ccattctccg gagactctgc ccctgtgccc ccaagtacct tctgtgccgc
    19801 cgaaaacagc ggtcatttcg ttcatggtca attctctcat ttcaaaactc cttttatcgt
    19861 caatcgaaaa attgaaaggg cagataccct atccttgctt ttacagacag agtatctgta
    19921 aaagggggta gtttcaggca gcttcagtgt ttatgttttt atgtagtgtt ttacaggtcg
    19981 tctgaagagg tttcagacaa cttctttatc tttcagcctc aagccttaca acctgcccga
    20041 catactataa atcggctcca atacccattc gtacagccgc cgtttctcgt gtaggatgtc
    20101 tgcttccaac gtcatgccga tttgcagcgg tttttcttca ccgtatgcag tgatggttgg
    20161 tttgtcgggt tttattttca cgagataaac aggttcgttg ctcttcgcca aatcggagga
    20221 taccatgccc aatcccgaca attcctgtct gcccagtgcc gtttttgcca ctgatacgac
    20281 actgccggaa gcaagcccga atttctgata gggatatgcc tgataacgta gcacaacctt
    20341 gtctttcggc ttgataaagc ctgctgcact gctggggata tatagatggg catataactc
    20401 ggtacgttcg ggaacaatgc tcaagagcag tttggaagga tcaacctgcc gtccgacttc
    20461 gacgttcggt attgctatat aacccgaccg tcctgcacgg atgatttgtt cagagcgcat
    20521 ttcaaaatcc aaaacttctt gagaaatatc ggcaatggtg cgttcaagct ggctttgttc
    20581 tgtctcatgc cgtttgggga ggctggccaa tgtcagattc tgcgtgcgga tttcctgaag
    20641 cagcccggct tcttctcggc ggtaggcatc aagtttggct ttctgctcta aaagctctgc
    20701 ctcgacattc atcgtttctt gttttgacac tgcatcattg gcggatagga aacgatattt
    20761 ccgcaacatt tcttccgcaa gtctaatgcg ccttttctgg ccgtctatct gttgcgaaat
    20821 atggagtttc tggttttcca aacgttcgac agttgcttta aggctgcgcg tttcattctc
    20881 gtgtatcagc ttcagacgac ccagttcctg ttctgccaac gttttcttca aaaccgcttc
    20941 cgttttcaac tgctgctgca cgctacctcc tgcgccgaaa cgtgaggtcg aaagcgcaaa
    21001 tagcttgtcg ccagccttga ccttttctcc atcttccacg aatttcgctg taattgtccc
    21061 cgtatccggt gcatacaccc tgattacgcc cgatgcaggt aaaatttgtc cctccattgt
    21121 tgtctttcgc gtatagttac cgaatatcaa aaacaggata atcagtaacg cagatatcga
    21181 tgcaaatgtc gtccacaggg aaaatgacaa cggtcgtgtc agaatcactt tacccgtcag
    21241 gctggtttgg cgggcaacag tcacttcagg gcggaagaaa ggttgcttgg gtctattcat
    21301 aaaattgaag ttaagaaagt ttcagacgac ccctagagat cgtctgaaca atgagaaata
    21361 tcagcagtaa tctgtaccgt cagtgtagcc gtttcctgat ttatctgctt ttgttgcggg
    21421 agcagttaat ccatgttcaa tctcaaagat tggtcttccg ttataaggag gtgcattaac
    21481 ggcatcattt acccaatcac gagtcagatt gtatacacca tttgcaccat atagcaccga
    21541 aaaaattttt cggcaggaaa tgaactgtcg ctgcagcagc aggtattgca acaaaatccc
    21601 cccatgtggg acctcctttg gttgtggcag cattagctac atttccagct atattgtctg
    21661 ttacaggacc tccccctgaa accagcttca attcatgaag ttgaagttca atcattttta
    21721 tactcctttt cttggttggt attcctaaaa attcggctaa caaaaacata tggcagatat
    21781 attgaaaaaa aattcaaagt accctgaata aaattcaaat tccaactata tttgttaatg
    21841 tagtcgagaa gaaacatatc tgataaagaa tatagcaatt gataacaagc tattactaat
    21901 attacgaaaa atataaactg cttccagttt ttcatagaat ccctcacaaa atttccagaa
    21961 aatctaactc tatcaactga tagatcaact tcctaacttc ttcatgtttt gcctgattga
    22021 agttaaccag tagatttttc aacaataacg gttcattctt accgatgtgt tctaacactt
    22081 ttttccccaa ctcatctacg cttatcttca tcccattccc aatcaaatat cccttttcca
    22141 acgtatccaa attattggca tttaatctca acctgacgtc gtctgaaagc ggagtagcgt
    22201 tgggattcgc gaactgttcg agatgaaaag cggtatcggt acgttctttg ccgagaaagt
    22261 cttcactgaa ggcttcgtaa ttgacggggt cggcaatcat ggcggcaatt tgtgcggcag
    22321 tatcgttgat acgcgtccta tcttgctccc agtctgagaa actgtggcgc agactttcta
    22381 tggtgggaaa tttcttcatt agccactcga ggtaattata gccgtttggt gggaaggtac
    22441 cgacagcgaa gtggaaggtt tcacagccga gcgggatagg tctgtgccac caaccgcgtg
    22501 ggatgtagag gacatcacct gcttcaagga taatatccat atcgatatgt tcaggaatgg
    22561 aaatatcagt atctttagtc tgttgcatat acaatggcat agggaaatca ggggcagtaa
    22621 gttgccaacg tttcttgccg aaaagctgga cggcatacat atctcgggcg tcccaatggt
    22681 ttttataaga ttcgtcgctg ccaaaagcaa gatatccact aacaatagta tgtgcgccgg
    22741 caaagcgggc gacttgacgg gcgatatggt ctgaaaacgg ctcgttgtta atatggttat
    22801 agactaacga cgcaccgttc ttcatatgtt cgtagataac ggatttaata aaacggtagc
    22861 gagttttgcc caaatcgtcg aaactttcga cgtattcttc tttaggaacg attgcgcctt
    22921 ttttacgcag atgaaacagc ggtgcggttg ggtctgctcg ttggtatatc tcgttgatat
    22981 atttacaaga tgcggcttcg agattccgaa ccgctccttt aaagagcttg ggcttttgat
    23041 acagataagt ctgtcggaac gttttaggac taatgccgaa gtcgagatgg atgcccatta
    23101 cttcccctta ctcagaaaat atttaaaatt tataatgtta catatagtta caaatatta
//
LOCUS       DAXFWM010000027        23084 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000027 DAXFWM010000000
VERSION     DAXFWM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23084
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.027"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            156..2398
                     /locus_tag="ACFI8B_001031"
                     /pseudo
     CDS             156..2398
                     /locus_tag="ACFI8B_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2523..4073
                     /locus_tag="ACFI8B_001032"
     CDS             2523..4073
                     /locus_tag="ACFI8B_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC3834278.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4497..6281)
                     /gene="lpdA"
                     /locus_tag="ACFI8B_001033"
     CDS             complement(4497..6281)
                     /gene="lpdA"
                     /locus_tag="ACFI8B_001033"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3834279.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPKPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6299..6733)
                     /locus_tag="ACFI8B_001034"
     CDS             complement(6299..6733)
                     /locus_tag="ACFI8B_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834280.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6834..8423)
                     /gene="aceF"
                     /locus_tag="ACFI8B_001035"
     CDS             complement(6834..8423)
                     /gene="aceF"
                     /locus_tag="ACFI8B_001035"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC3834281.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     SLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSAAPAAVPTSASPAAAK
                     IDEAAFAKAHAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKP
                     AAAGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEE
                     ADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNY
                     FNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTIS
                     SLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGA
                     AGMRFTVFLANLLKDFRRITL"
     gene            complement(8573..11236)
                     /gene="aceE"
                     /locus_tag="ACFI8B_001036"
     CDS             complement(8573..11236)
                     /gene="aceE"
                     /locus_tag="ACFI8B_001036"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC3834282.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRLFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11933..13645
                     /locus_tag="ACFI8B_001037"
     CDS             11933..13645
                     /locus_tag="ACFI8B_001037"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC3834283.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13708..14364
                     /locus_tag="ACFI8B_001038"
     CDS             13708..14364
                     /locus_tag="ACFI8B_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC3834284.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14374..14829)
                     /gene="ruvX"
                     /locus_tag="ACFI8B_001039"
     CDS             complement(14374..14829)
                     /gene="ruvX"
                     /locus_tag="ACFI8B_001039"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC3834285.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14822..15370)
                     /locus_tag="ACFI8B_001040"
     CDS             complement(14822..15370)
                     /locus_tag="ACFI8B_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC3834286.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15372..15932)
                     /locus_tag="ACFI8B_001041"
     CDS             complement(15372..15932)
                     /locus_tag="ACFI8B_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC3834287.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGASFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            16258..18060
                     /locus_tag="ACFI8B_001042"
     CDS             16258..18060
                     /locus_tag="ACFI8B_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC3834288.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKA
                     QISRFVSGNYKNSRPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKG
                     TEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEKEAAQQKAEARRAE
                     MSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVF
                     YSTAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAG
                     SKIGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            18187..19662
                     /locus_tag="ACFI8B_001043"
     CDS             18187..19662
                     /locus_tag="ACFI8B_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC3834289.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            19992..22019
                     /gene="uvrB"
                     /locus_tag="ACFI8B_001044"
     CDS             19992..22019
                     /gene="uvrB"
                     /locus_tag="ACFI8B_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC3834290.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLIEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(22197..22955)
                     /gene="cah"
                     /locus_tag="ACFI8B_001045"
     CDS             complement(22197..22955)
                     /gene="cah"
                     /locus_tag="ACFI8B_001045"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFC3834291.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
BASE COUNT         5737 a         5769 c         6216 g         5362 t
ORIGIN      
        1 caaaaataaa ccaatattta acagtcggat agctccgcct gattaagttt ttcggtaata
       61 gttgtgatta tcaaatataa ttgccgcccg aaatatccga taagtttgtt tgttttatct
      121 ttaattcccc aatacttaaa aaagagagcg ttttcatgaa caaaaaactt gccttaatgc
      181 cgctgttgat tttgagcgcg ttttcatctg ccgccgacaa tactccgcag catggcgaac
      241 tcggacaggt tcatgtccgc gccgatgcca agcgcgtcaa agccgcgcat tcttactcca
      301 tcgccagcga tggcgacttg cgcgaccgcg taaatttggg cgtattgggt aaagccaacg
      361 cgtttaccgc gccgattacc gtcgtcaact acgacgaaca agccttcaac aataccgagg
      421 cgcgtacttt agtggatgcc gtagccaaaa aagacgcttc cgtttgagag tttggcggcg
      481 aaagcaacac gctgaccggt ctgtatttcc gtggttatca gcttgatgcg cgccaattca
      541 gcgtaaacgg cttggcagga atgtatggca cgcaaggcac ggcgagcgtg caagtcggct
      601 tcgcacaact gattaaaggc gcgtccaccg ccgtaaacgg cataccctga aggcgcggta
      661 tccggttccg tcaacatcga aaccaaaaaa gccgccgacg aaggcaaccg caaaatcggt
      721 ttggcccggt tcggcaacaa ccgcgcccaa ggtaggttcg acttagggca acgcttcggc
      781 gaaaacaaag cattcggcgt gcgtgccaac ggcaaactgc gccacggcga caccccgcgc
      841 cacggttaca gagaagacaa caaagaattt gccttaaatg ccgattatcg cggcgaaaaa
      901 ctgcgcgtga cgttcgactc catctacgcg aaacgcaaaa tcaacggcgg ccgcgcgcgt
      961 atgcaggata tccaaaacgc cggcggacgc ttgttcgacg cgcccgacgg caaaatcaac
     1021 ctgctgcctt cttggaactg gcaaaatacc gtcggcgaaa ccaatatgct gacgtttgaa
     1081 tgggatgcgt ttgacaatac ctaaattacc ggcggtatcg gctacaacaa agcgcgttat
     1141 tacggtacgc tgatttcccc aaccgtttgc ggtacaagcg gtgcgagcag ccaaaccaaa
     1201 acctgcatgg ctgccaacca ataccacacc ggcacggcgc gcctgaccga ccaatatttc
     1261 cgtactttga gtatgaacct gaccgcacgc ggcgaatttg aaaccggccc ggtcacgcat
     1321 aactggagta cggctttcga ccgcgttatc cgccagcgga aaaccataaa cggcagcggg
     1381 aacggcaaca gcaagataga agtgaaggca aacgaaaata ttgcgaatca attggcatcg
     1441 ttcaaagccg actatcccaa ttcttgggcg aactcggcca acttggatgc caatatcaaa
     1501 gtcaacagct tggctttgtc cgatactttg ggattcgttg ataacaaata ccctctgact
     1561 ttgggcggac gtttccaagc ggttgaatac actgataaga agaaatcaca gagcggcgat
     1621 gccaaacgtt tcagcccgat gctgatggcg gcgtgggtgc cgcaacctga tttggtcgtg
     1681 tgcggcaact atatggaaga cttggagcgt gccgatatca aaaccgatga ttccggcgaa
     1741 acgacaatgg cgaagccgcg cgtcagccgc cagtttgaaa tcggcgtgcg taaaaactgg
     1801 ggcgattttg taaccacgtt gaacgcgttc caaatcaaac gcccgggcta ctggcgcggc
     1861 aataccaaaa aaggtactga ttttgcagca tataaagcag caggaggcgc agccggcgac
     1921 gagcaaggta tggaacgcaa tcgcggtatt gagttcaatg cctatgccaa cctgttgaac
     1981 aaaaccctgc gcccgacttt cggcgtgatg tatctgcaat cgtccgtgaa gaattatccg
     2041 aatgcgcgcg atatgctggt taacggcgta caagtcgcca acccgcgcgt gattgtcaaa
     2101 acaggcgttg aacgggatac accgtttgcc aaaggtttga gcttgaacgg caatgtttcg
     2161 tatttcggca agtcttatca agacacgcaa aaacaatacg ccttcccgtc ctataccttg
     2221 gttgacgtag gcgcgctaca aaaccaagct gggcaaaaac accctgaccg tcagcagctc
     2281 ggtagaaaac ctgttcaaca aaaactactg gcaggtacag cgcggacaat ttgaccgtag
     2341 tttcgccgtc gtcggtctgc cgcgtactta ctggctgaag gcggaattgg atttctaagc
     2401 tgcctccgtt tccgaaaatg ccgtccgaac aggtttcaga cggcatttct tttaaagaaa
     2461 tatggcggga tatgcttaaa taatgctatt ctagtacctt tgaaacctgc ttgtttgaag
     2521 tgatgaaaaa acctttgatt tcagttgcgg cagtattgct cggcgttgct ttgggtacac
     2581 cttattattt gggtgtcaaa gcagaagaaa gtctgacgca gcagcaaaaa atattgcaga
     2641 aaacgggctt tttgaccgtc gaatcgcacc agtatgatcg aggctggttt acctctacgg
     2701 aaacgacggt catccgtctg aaacccgagt tgctgcataa tgcgcagaaa tacctgccgg
     2761 ataacttgaa aatagtgttg gaacagccgg ttacgctggt aaaccatatc acgcacggcc
     2821 ctttcgccgg cggattcggc acgcaggcgc acattgaaac cgagttcaaa tacgcgcctg
     2881 aaacggaaaa agttttggaa cgcttttttg ggaaacaagt tccggtttcc cttgccaata
     2941 ccgtttattt caacggcagc ggtaaaatgg aagtcagtgt tcccgctttc gattatgaag
     3001 aactgtcggg catcaggctg cactgggaag gcctgacggg ggaaacggtt tatcaaaaag
     3061 gtttcaaaag ctaccgcaac agctatgatg cgcccttgtt caaaatcaag ctggcagaca
     3121 aaggcgatgc cgcgtttgaa aaagcgcatt tcgattcgga aacttcagac ggcatcaatc
     3181 cgcttgcttt gggcagcagc aatctgactt tggaaaaatt ttcgctcgaa tggaaagagg
     3241 gtgtcgatta caacgtcaaa ttgaacgaac tggtcaacct cgttaccgat ttgcagatcg
     3301 gcgcgtttat caatcccaac ggcagcatcg caccttccaa aatcgaagtc ggcaagctgg
     3361 ctttttcaac caagaccggg gaatcgggcg cgtttatcga cagcgaaggg cggttccgtt
     3421 tcgatacgtt ggtgtacggc gatgaaaaat acggcccgct ggacatccat atcgctgccg
     3481 aacacctcga tgcttctgcc ttaaccgtat tgaaacgcaa gtttgcacaa atttctgcca
     3541 aaaaaatgac tgaggaacaa atccgcaatg atttgattgc ggcagtcaaa ggcgatgctt
     3601 ccggattatt tacccatgac ccggtactaa atatcaaaat tttccgtttc accctgcctc
     3661 agggaaaaat tgatgtgggc ggaaaaatca tgtttaaagg catgaagaag gaagatttga
     3721 accaattggg actgatgtta aagaaaaccg aggcaaacat cagaatgagt attcctcaaa
     3781 aaatgttgga agatttggcg gtaagtcagg ctggaaatat tttcagtgta aatgccgaag
     3841 atgaggcgga agccagagca agcattgccg atattaatga aacattgcgc ctgatggtgg
     3901 acagtacggt ccaaagtatg gcaagggaaa aatatcttac tttagacggt aatcagattg
     3961 atacggtcat ttcccttaaa aacaacgccc tgaagttaaa cgggaaaacg ctgcaaaatg
     4021 aacccgatcc tgattttgac gagggagata tggtttccgg ccagccgcat taatgccgtt
     4081 tatgccaaat gccgtctgaa atgttcagac ggcatttttg tcggtctcga aataggatgg
     4141 attttcatgt ttcaaaaaca attgattgtc tctaatccga tatcaggaca cgcttccaat
     4201 gtttagatat ggaattcgga cgggataggg gaactattga aatccagttg aaatctgtta
     4261 ttggaaagca tggaggaaaa ataaaaggct gtattttaat atagtattga taggcatgct
     4321 tagcttcaag gtattcaata gtgtaggtgt ctgcctaact ggaaaataat cgtcttttcc
     4381 cttgcaacca gttaaaattg acagatggtt aaaacacagc caaataaaaa tgccgcctga
     4441 agggttcaga cggcatttgt tcggataaac cttatcggct gcttagagca tcaggtttat
     4501 tttttctttt gaggaggcag gtcggtacat acacccaaag ccacttccgc agccataccg
     4561 atggattcgc ccaaggtcgg gtgcgggtgg atggttttgc cgatgtctgc cgcgtcgcag
     4621 cccatttcga tggcaaggca gacttcgccg atcatgtcgc caccgttcgg accgacaatg
     4681 ccgccgccga tgatgcggcc ggtttcggca tcgaaaatca gcttggtaaa gccgttgtcg
     4741 caaccgttgg caatcgcacg accggaagcc gcccatggga agttggcttt ggtgattttg
     4801 cggcctgatg ctttggcaga caattcggtt tcaccgaccc atgccacttc gggggaagtg
     4861 taggcaacgc cgggaataac gcgtgcgtcg aagtaggctt tgtggccggc acagttttca
     4921 gcggcaacgt ggccttcgtg aacggctttg tgtgccaaca tcggttgacc gacgatatcg
     4981 ccgatagcgt agatgtgcgg tacgttggtg cgcatttgtt tgtcaacttc gatgaagcca
     5041 cgatcggtaa cggcaacacc ggctttttct gcgctgatga gtttgccgtt aggcgcacgg
     5101 cctgccgcga ccagtacggc atcgtaacgt tgcggctctt taggtgcatt tgcaccttca
     5161 aaggtaacgt aaacgccgtc ttctttcggc tcgactgcaa cggttttggt gttgaccata
     5221 atgttgtcaa aacggtattc gttttgtttt tgccatactt taaccaagtc gcggtctgcg
     5281 ccttgcatca ggccgtccat catttcaacc acatccaggc gagaacccag cgtgctgtaa
     5341 accgtaccca tctcgaggcc gataatgccg ccgccgataa tcagcagttt gcccggtact
     5401 tctttcagtg ccagtgcgcc gctggaatcg atgatgcgcg gatcttcagg aatgaaaggc
     5461 agtttggtta cgcggctgcc tgctgcaatg atacagtttt tgaaggcaac gatttttttc
     5521 tcgccggtag gggctgcctg ttcgtacgcg tcgccggcag tcagcgacac ttccaagtgg
     5581 tgaggatcca agaattgccc gtcgccttgg ataacgtcca ctttacggct tttcgccata
     5641 cctgccaaac cacccgtgag gcgggaaacc acgccgtctt tgtaggcgcg aagcatatcg
     5701 atgtcgagtt ccggcttggg gtatttgata ccgttggcag ccaagtggcg cacttcgtcg
     5761 ataacggcgg cattgtgcaa caaggctttg gaagggatac agccgacgtt caggcaaacg
     5821 ccgcccaaag ttttgtaacg ctcgacgatg gcgactttca agccttcatc ggcagcggca
     5881 aatgcagcgg agtaaccgcc gggaccgccg cccaatacga ccacgtcgta ctcggcatcg
     5941 gcagcaccgc cgaattgcgc ggcttgcgga gcaggagcgg cagctttggg tgcttcttgc
     6001 gccggggcgg cagccgcttc ggctttaggg gcggcagccg caccttcggc ttcgacgacg
     6061 acaatcaaac cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgatcacg
     6121 cccgcaactt cggcaggcac gtccatcgtc gctttgtcgg tttccaaggt aatcagggtg
     6181 tcgtcaacgg cgatggtgtc gccaacgttc acttcaaccg cgataatatc tacattttcg
     6241 tgtccgccaa tgtcgggcac tttcaattca actaaagcca ttatattgac ctttcaactt
     6301 agttaatttc tatcaactct ttagcaataa tcacttcttc aggaatacag ccacaatctt
     6361 cagctctgat tgttacttcc ttctcatctg ggttttctgg atgttcaacc tcatattcaa
     6421 aaatagaata ttgaccaaac aaatctctat ttaaaacata tatatagcca ttttcgatgc
     6481 cggagcttgt tgcaaatttc ttggcaattt ccttgtctgt tgtcgtagat atataacatc
     6541 cgtcatatag atctgtttca atttgatggg cgtaaactgc attcttcaca cttggaccat
     6601 gtgtagcttt accatcatat ttaaacttac catcataacg aattgcaact tcagctttat
     6661 tacctttagg ttttaactgt ccattatttt gctcatcttg ttggcaacta atgcctctat
     6721 ataaataaga aatttcccat aagttattaa tttaaagttt gaatttaaca ccatcactca
     6781 tcaatctcaa gcaatccaaa cattatcaga ctgcttgaga gggatgtttt attttataag
     6841 gtaatgcggc ggaagtcttt caacaggttc gccaggaata cggtgaagcg cataccggcg
     6901 gcaccgtcga tgacgcggtg gtcgaaggac aggctcaacg ggcacatcag gcgcggggca
     6961 aactctttgc cgttccaaac cggtttgatt tgggatttgc acacgcccaa gatagcgact
     7021 tcgggagcgt tcacaatcgg cgtgaaacct gtgccgccga tgccgcccaa gctggaaatg
     7081 gtaaagcacg cgccttgcat ttcttgcggt ttgagcttgc cttcgcgggc ttttttggac
     7141 aattcggtca attcttggct gatttgtttc aagccttttt gatccacgtc tttgatgacg
     7201 ggaacaacca agccgttcgg cgtatcggct gcgaaaccga tgttgaagta gtttttcagc
     7261 accaggttgt cgccgtccaa agaagcgttg aattcgggga aggctttcag cgcggaaacg
     7321 gaggctttga tgatgaacgc caacggggac agtttcacgc cttcgcgttc ccattctttg
     7381 ttcagctgtt tgcggaattc ttccagttcg gtcatatccg cttcttcgtg tacggtaacg
     7441 tgaggaatca ccacccagtt gcgggacagg ttttgaccgg agattttctt aatgcgggac
     7501 aattctttaa cttcgacatt gccgaatttg gagaagtcca ctttaggcca cggcagcaag
     7561 tccagaccgc cgcccaaaga tgcgccggct gcggcaggtt ttgccgcgcc gccctgcatt
     7621 acggatttca caaaggcttt gatgtcgtct cccatgatac ggcctttcaa gccgctacct
     7681 ttgacttggc ccaaatccac gcccaattcg cgcgccagtt tgcgcgcgga aggaccggcg
     7741 tgtgctttgg cgaaagcggc ttcatcgatt ttggcagcgg caggcgaggc agatgtaggc
     7801 acggcggcgg gtgcggcaga tgtaggcacg gcggcgggtg cggcagcttg agcggcaggc
     7861 gcaggagctg ctgcggcaga gccggcggtt tccacttcga taatggcaga gccttcggac
     7921 actttgtcgc cgacttttaa ggatacggct ttaacgacac cggcagcggt acaaggtacg
     7981 tccattgtcg ctttatcggt ttccaaagta attagcgtgt cgtcttcggc aacggtgtcg
     8041 ccaactttga tttcaacggc gattacatct acatcggtat ggccgccgat atcgggaacg
     8101 gctacttgaa cggttgcacc gcctgcggca gcgggtgcag gttgtgcttc ggcggcagca
     8161 gccggggcgg cttcggcagc ggcggcaccg gtttcaacgg tcaaaattac gccgccttcg
     8221 gagattttgt cgccgacttt gacttttact tctttcacga caccggccgc atcggcaggc
     8281 acatccatcg tggctttgtc ggtttccagt gtaatcaggg tgtcgtcaac ggcgatggtg
     8341 tcgcccgctt taacttctac ggcgatgatg tcgacgcttt cgtgaccgcc gatatcgggg
     8401 actttgattt ctacgatact catttgagtt cctttaatct ttttcagttg gatgccgtct
     8461 gaaagctgct tcaggcggca tcggtacggt ttgtctgttc aaaccattcg ggcattcggt
     8521 tttcagacgg cctgatgttg gcatgaaaca aacaggccgt ctgaaacatc aatcagcgtt
     8581 tccagctagg ggctgaatcg gctttgatgc cgtatttctc aatggcttgt tgaacggttt
     8641 ctttgctgac tttgccttgt tccgccaatg cggccaatgc tgccacggca acgttgtagc
     8701 ggtccacttc aaagaggcgg cgcaggttgg cgcggctgtc ggaacggccg aaaccgtcag
     8761 tgcccaagac atggtagtcg ttcgggatgt acgcgcggat acggtcggca tagctgcgga
     8821 tatagtcggt agcggcgata accggaccgt catgaccttg cagttgggaa gtaacgaaag
     8881 gcactttttc agcttccagc ggatgcaggc ggttgaagcg ttcggtttcg atggcgtcgc
     8941 ggtgcagcag gttgaaggac gggcaagacc agatgtctgc ttccacgccg aagtcggctt
     9001 tcagcagctc ggcaccggca atcacttctt gcagaatcgt accggaaccc atcaattgga
     9061 ctttcttgtc gcctttgccg ccggctttca gcaggtacat acctttgagg atgtcttgtt
     9121 ccgcaccttc cggcatatcg ggatgggtgt agttctcgtt catcagggtg atgtagtaga
     9181 acacgtcttc attattggca tacatacggc gcagaccgtc ttgtacgatg acggccactt
     9241 cgtattggaa ggtcgggtca taagatacgc agttcggaat caggtcggcc tggatgtggc
     9301 tgtggccgtc ttcgtgttgc agaccttcgc cgttcagcgt cgtacggccg gcagtaccgc
     9361 ccagcaggaa gccgcgcgcg tgcatatcgc ccgccgccca agccaagtcg ccgatacgtt
     9421 ggaaaccgaa catggagtag tagatgtaga acggaatcat cgcgaagtcg ctgttggcgt
     9481 agctggtcgc agccgcaatc cagtcggcca tcgcgcccgg ttcgttaata ccttcttgca
     9541 agatttgacc gtcgacggat tctttgtaga acatcagttg gtctttatct tgaggcgtgt
     9601 attgttggcc tttgggattc caaataccgt attggcggaa cataccttcc atgccgaaag
     9661 tacggctttc gtcgggaacg ataggtacga cgcgtttgcc gatttttttg tctttcagca
     9721 aagtggacag gatgcggacg aatgccatcg tggtcgagaa ctcgcgttca ccgctggatt
     9781 tgagttgtgc gtcgaatgcc gacagctcgg gcacttccaa tacttcctgc gtcggtttgc
     9841 gttgcggcag gtagccgccc aaagcatcgc ggcgtgcgtg caggtatttg tattcttccg
     9901 tatcgggggc aaaagtcagg taaggcagat cgccgctttc gatttgttcg tcggtaaccg
     9961 gaatgtcaaa gcggtcgcgg aattgtttca gggacgcttt gtccattttt ttggcttggt
    10021 gggcaacgtt ctgaccttcg ccggatgcgc ccataccgta acctttaatg gttttcgcca
    10081 agataacggt aggtttgccg tcggcatggt tcgctgcgcg gtcgtaggcg ttgtacactt
    10141 tttgaggatc gtggccgccg cggttcaatg cccagagttg ctcatcggtc atatcggcaa
    10201 ccaatgcttt cagttcgggc gtattgaaga agtgttcgcg cacatacgcg ccgtctttgg
    10261 atttgtaagt ttggtagtcg ccgtccaaac attcttccat acgttggcgc aggataccgt
    10321 ctttgtcttt cgccaagagg cggtcccaac ggcggcccca aatgactttg acgacattcc
    10381 agccggcacc ggcaaagttg ccttccaatt cttggatgat tttgccgttg ccgcgcaccg
    10441 gaccgtccaa gcgttgcagg ttgcagttga tgacgaaaat caggttgtcc aagccttcgc
    10501 gtgcagccag cgcgattgca ccttgggatt cgggttcgtc catttcgccg tcgccgcaga
    10561 aacaccatac tttacggcct ttggtttttg ccaagccgcg tgattccaag tatttcagga
    10621 aacgcgcctg ataaatcgcc atgatggggc ccaagcccat ggatacggtc gggaactgcc
    10681 agaagtcggg caagaggtgg gggtgtggat aggaaggcag accgtgtccg tccacttctt
    10741 ggcggaagtt gttcagctgg tcttcggtca gacggccttc gacgaatgcg cgtgcataga
    10801 tgcccggggc aacgtgacct tggaagaaga ccaaatcgcc ttcttcacct tcacctttgg
    10861 ctttccagaa gtggttgaaa ccgacttcgt acatggtggc ggcagattgg aaagatgcga
    10921 tgtgcccacc cagttccaaa tctttcttgc cggcgcgcaa tacgatggcg gcggcgttcc
    10981 agcgtacgaa tgcgcgaatg cggtgttcga tgttttggtc gcccggaatg cctttttcgt
    11041 tttcaaccga aacggtattc aaatacgggg tggtcgtgcc gtggggcata cgtacgcctt
    11101 tgtcgcggca gtatttgagc aggttttcca agaggtattg cgcgcgttcg ccgccttcat
    11161 attcgaggac ggagcttaac gcgtccagcc actcttgggt ttcgataggg tcaacatcgt
    11221 gtaattgggt ggacatagta tctatccttt atgttgagtg tttaatcgga agggagggtg
    11281 tagccaaccc ctttattttc gtttgtgaaa aataataagc aaaaacgaaa atttctaaat
    11341 caaaccgaaa caaaataatt taagtttaat gttttcaaaa aattataaaa gtcttggaag
    11401 caagggaaaa cttgaaaaaa aatgttcggt ctgctccgca cgaaatccta tcagcaatcg
    11461 ggtctgctgg caaataacca tgcttttaaa agcaggtttt atactgaagt tggcgtttgg
    11521 taaggacagg tctttttgat tgcgaaaaca ttctgtttaa ttttggttat attttttcgg
    11581 aattgtggga tatcgaatcg ttatgtctct gtcggcaatt ttcctattgc agaaaatcta
    11641 tacacaagct ttgtggacaa ccgtgttgat aagccacggc ttgtgttaaa aaagtcttac
    11701 gcaacaaatg tacgcttatt ttgattaaaa attaggcaaa tttaaatcta taaaaatcat
    11761 gtaattatgt tcaatatgat gtttctggtc atgtttttgc cgtctgttat ggagtttgtc
    11821 ccgcgtttgg ggcagggggt gaattttgga ggttggtgca aaacctgctt ttttcgcgtt
    11881 acaatctgca cccgatttac ttttgaatct gcctgtttgg aaaaatattt ttatgaaagc
    11941 cagccaattc tttatctcta ctttaaaaga agcccctgcc gaagccgcgt ttgccagcca
    12001 caagctgatg attcgcgccg gtctgattaa agccaacgcg tccggtcttt atacttggat
    12061 gccgatgggg ctgcgcgtgt tacgcaaagt cgaaaacgtc gtgcgcgagg aaatggcgcg
    12121 cgcgggcagc gtggagctgc tgatgccggt ggtgcagcct gccgaactgt ggcaggaatc
    12181 cggccgctgg gagttttacg gtaaagaact gctgcgcctg aaagaccgcc acgaacgcga
    12241 tttctgcatg ggcccgacct gcgaggaagt catcgccgac atcgtgcgca aagaaatcaa
    12301 cagctacaaa caactgccga aaaattttta ccacatccaa accaaattcc gcgacgaagt
    12361 gcgcccgcgt ttcggcgtga tgcgcgcgcg cgaatttgtg atgaaagatg cttattcctt
    12421 ccacgccgac tacgcctcgc ttcaggcgac ctatgatgcc atgtatgacg cttactgccg
    12481 catctttacc cgtctgggtt tggcgttccg ccccgtcgcc gcagacaccg gcagcatcgg
    12541 cggtacgggt tcgcacgagt ttcaagtgtt ggcggaaagc ggcgaagatg tgattgcata
    12601 cagcgacact tccgattacg ccgccaatat cgagttggca ccgaccttgc cgcttaaagg
    12661 tgaacgtgcc gccgctcagg ctgtgttgac caaagtacat acaccaaacg tcaaaaccat
    12721 tgagtctttg gttgaattcc tgaatattcc ggttgaacaa accctcaaat ccatcgtggt
    12781 tgaaggcgaa aacgaaggcg aactcgtcct actgctgttg cgtggcgacc atgagtttaa
    12841 cgacatcaag gcagaaaaac tggcgggcgt aaaatcgcca ctgactatgg caagccctgc
    12901 cgcgattgtt gaacaattcg gcgcaaacgg cggttcgctc ggccccgtcg gcttcacagg
    12961 caaagtctat gccgatttcg ctaccgaaaa aggcgcggac tgggttatcg gcgcaaacga
    13021 agacgactac cactataccg gcttcaactt cggccgcgat gccgccgagc ctgaattcgt
    13081 cgatttgcgt aatgtggtcg aaggcgacga aagccctgac gggcaaggcc gtctgaaact
    13141 ggcgcgcggc atcgaagtcg gacatgtctt ccagttgcgc ggcaaatata cccaagctat
    13201 gaacgtcagc ttcctcgaca ataacggcaa atcgcaaatc atggaaatgg gctgctacgg
    13261 catcggcatc acccgcgtcg ttgccgccgc catcgagcag aataacgacg aaaaaggcat
    13321 catttggacc aaagcgatgg cgccgtttga agtcgtcatc gtgccgatga actacaaaaa
    13381 atccgacact gtgcgtgaag ccgccgacag aatctatgcc gaattgctgg cggcaggcgc
    13441 ggatgtgctg ctggacgacc gcgacgaacg cgcaggcgta ttgctgaacg attccgagct
    13501 tctcggtatc ccgcaccgca tcgtcatcgg cgaccgcgcc ttgaaagaag gcaatgtcga
    13561 atacgccgaa cgccgcgaca acgaagcgca ggcagttgca atcggagaaa ttgttgcgcg
    13621 tgtaacagct tcattaaatg cgtaagtctg gtcaaatgcc gtctgaaaat aatttcagac
    13681 ggcattttta ttttggggga aagtgaagtg gcttcagtat ttttagaagg agaagcagtc
    13741 gaggtcggca atattttctg tatcggcagg aactatgccg cacatgtcga agagttaaaa
    13801 aacgaaatac cttcagaacc tgtggtgttt atgaagccgt caggcagcat tttgaacagc
    13861 ggcggtacaa tcttgctgcc cgagttcagt agggatgtgc agtttgagtg cgaactagtg
    13921 ttgctgattg gccgggattc agacggcacg ggcgagggtg aagatatttt gggatgcgtg
    13981 gcggggtacg gcgtgggttt ggatttgacc gcgcgggaca tccaatgccg tctgaaggcg
    14041 caggggctgc cctggctgaa ggcaaaaggg ttcaggcatt ctgcgtgcgt gtcggatttt
    14101 gcggcggcag acagaatcgg taatcaagat aaggtgttgt tttcattaaa acaaaacggc
    14161 gtattgaaac agcgcgggga gactggtttg atgatttacc cgattcggga aattttacac
    14221 aaactggcgg cggattacgg tttggggaaa ggggatttgg tgtttaccgg aacgccctcc
    14281 ggcgtgggcg caatcggtgc gggggacaat ttggcattgg agctggacgg cttggtgtgc
    14341 gcctctttca cgatagggtg ttaaccgcgc cgcttaacct tcacgcccgt tgaaacattc
    14401 cgccgggccg ccctcgagaa aaccgtgcag gatggcttgc gccgccactt ggtcgagcac
    14461 cgatttgcgt tttttgccca agacctgtgc ttccgaaagc aggctttcgg catagacgga
    14521 cgacagccgt tcgtcaaccc aatagacggg gagattgaac ctgccgttca gcctgcgtcc
    14581 aaacttgcgc gacaggtgcg tcatttcatg ttccgtgccg tcggcatgca cgggcagtcc
    14641 gacgacaaaa taacgcggct gccattcttg aaccagcttg gcgattgccg cgaatttttc
    14701 atcgttgctg ccgccggtaa cggtggccag cggatgggac agccccaatt ccgcatcgcc
    14761 ctgcgccacg ccgatgcgcg cttcgccgaa gtcgaatgcc agtgccgttc cttttggaat
    14821 tttatgcatg gccggctccc gaaaacaggg cgagcgggtc gatgccgagt ttggcgaatg
    14881 cggcggcgta acggtgttcg taggggatgt cgaacaggat gtgttcgtcg gcgggaacgg
    14941 tcagccacgc attgtcggca agttcgcgtt cgagctgccc tttgctccag ctcgaatagc
    15001 ctatgctgat caaggctttg tcaaccgcgc cttcgcgtga aatgttttca agcacatcgc
    15061 gggaagaggt cagcgcgatg ccgtctgaaa cgccgatgct gctttgccag ttgccgatcg
    15121 gagtgtggac gacgtaaccg cgctcgacct gcaccggccc gcccatcatc acgctgtcgt
    15181 gctgcatccg catggggatg tttttgccgg tggcggaaaa aatcatgtcc atcgtaatcg
    15241 gagagggttt gttgatggcg ataccgagtg cgccgtcttc atcgtgtttg cagatataga
    15301 cgaccgattg tgaaaaaaac gcgtcttcca tatcgggcat ggcgacgaga aagtggttgg
    15361 acaggttcat atcagcgttt gtcgggattt tttatcgggt tggaaataat gcaggcgccg
    15421 gcaagcagtt tgccggaagt atcggcttgc accccgtcgg tttgcggtat gccgctgccg
    15481 aaacaggaaa ccgcgcttaa ggaatttttc ggcgatcctt ggacgatttt atcgctgtaa
    15541 accaaatagg cgaaggcttt gcgtttgggg tcgtaataac ggacaatctg ccggctcttg
    15601 aacgcgaagc tcgcgccgcg cttgaaaact tctttcggtt tgcgcacggc ggtttcgtca
    15661 aaagaaatcg aagatgccgt ctgaacgcac gaaaccgatg cgtcggacgc gtcctcttcc
    15721 aaattgacca tttccttcaa gccgcctttt tttgcatacg aaatataaca ggcaacccct
    15781 tgaacgtcgg gatcgtcgaa tccttccact tcgatacggt cgtttttgcc caacatgttg
    15841 aaaacggtac ttgcccgtcc gattttatcg gtttcgccgc tgccgcaggc agtcggcagg
    15901 acggcggcag acagcagtag cagtctgttc atcaaaattt ccttatcggt tgaatccccg
    15961 ttttccggaa ggcggcaggg tttgctttgt ttggaggggc gcacgctccc aaatcgggca
    16021 acatacggta cgcagcttac atgtgctacc ctgtgtgtcg gaacacaata tccgttgcag
    16081 cattgaaaat atgccgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa
    16141 gaaggtgcaa gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa
    16201 cgtccggctg ctttcgtgca gccttcagta ttatccaact tttccgaagg tcgaaccatg
    16261 cgctacaaac cccttctgct tgccctgatg ctcgtttttt ccacgcccgc cgttgccgcc
    16321 cacgacgcgg cacacaaccg ttccgccgaa gtgaaaaaac aggcgaagaa caaaaaagaa
    16381 cagcccgaag cggcggaagg caaaaaagaa aaaggcaaaa atgccgcagt gaaagataaa
    16441 aaaacaggcg gcaaagaggc ggcaaaagag ttcaaaaaaa ccgccaaaaa ccgcaaagaa
    16501 gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataaggaa
    16561 tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa agaaaaaaac
    16621 gcaaaaacac agcctgaaaa caaacaaggc aaaaaagggg caaaaggaca gggcaatccg
    16681 cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagc ccgttccgac
    16741 aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa tgccaaaacc
    16801 gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa
    16861 gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct caaacagctt
    16921 caggaagagc gtatccgcca agaacgtatc cgccaagagc gtatccgtca ggcgcgcggc
    16981 aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg acaaattcca aaaactcaat
    17041 accgagctga accgtttgaa aacggaagtc gccgctacga aagcgcagat ttcccgtttc
    17101 gtatcgggga actataaaaa cagccggccg aatgcggttg ccctgttcct gaaaaacgcc
    17161 gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt acgtaaacgc ctccaatcgg
    17221 gaagttgtca aggatttgga aaaacagcag aaggctttgg cggtacaaga gcagaaaatc
    17281 aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct gctgaaaaaa
    17341 cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca gacagaatgc caaaatcgcc
    17401 aaagatgccc gaaaactgct ggaacagaaa gggaccgagc agcaactgaa caagctcttg
    17461 agcaatttgg agaagaaaaa ggccgaacac cgcattcagg atgcggaagc aaaaagaaaa
    17521 ttggctgaag ccagactggc ggctgccgaa aaagccagaa aagaaaaaga agcggcgcag
    17581 cagaaggctg aagcgcgacg tgcggaaatg tccaacctga ccgccgaaga caggaacatc
    17641 caagcgcctt cggttatggg tatcggcagt gccgacggtt tcagccgcat gcagggacgt
    17701 ttgaaaaaac cggttgacgg tgtgccgacc gggcttttcg ggcagaaccg gagcggcggc
    17761 gatgtttgga aaggcgtgtt ctattccact gcgcctgcaa cggttgaaag cattgcgccg
    17821 ggaacggtaa gctatgcgga cgagttggac ggctacggca aagtggtcgt gatcgatcac
    17881 ggcgagaact acatcagcat ctatgccggt ttgagcgaaa tttccgccgg caagggttat
    17941 acggtcgcgg caggaagcaa aatcggcacg agcgggtcgc tgccggacgg ggaagagggg
    18001 ctttacctgc aaatacgtta tcaaggtcag gtgttgaacc cttcgggctg gatacgttga
    18061 tcggattgcc gaaacggcag gctttatgca aatcgtgccg atatggtgcg ctgttccgtg
    18121 aagtttgccg tttgccgtac ctgtgttttc cggtttttgg aaatcaagag agaaagttaa
    18181 agaagaatgt cgaaacctgt ttttaagaaa atcgcacttt atactttggg tgcaatcagc
    18241 ggcgtggccg taagtctggc ggtgcagggt tttgccgccg agaaggacgg gcgggataac
    18301 gaagtcctgc cggtgcaatc catccgtacg atggcggagg tttacggtca gattaaggca
    18361 aactactatc atgacaaacc cgatgccgat ttgtttgaag gtgcgatgaa gggtatggtg
    18421 gccggtttgg atccgcattc cgaatatatg gataaaaaag gttatgccga gataaaggag
    18481 tccaccagcg gcgaatttgg cggcttgggg atggaaatcg ggcaggaaga cggttttgtc
    18541 aaagtggttt cgccgattga ggacacgcct gccgaacggg cggaggtgaa aagcggcgat
    18601 ttcattgtga aaatcgataa tgtttcgacg cgcggtatga cggtcagcga agcggtgaaa
    18661 aaaatgcggg gcaagccggg tacgaagatt actttgacgc tgtcgcgcaa aaatgccgac
    18721 aagccgatag tcgtcaacct gacccgtgcc attattaaag tgaaaagcgt ccgccatcac
    18781 ctgatcgaac ccgattacgg ctatatccgc gtgtcgcagt tccaagagcg gacggtcgaa
    18841 agcgtcaata ccgccgcaaa agagctggta aaggaaaata aaggaaaacc gctcaagggg
    18901 ctggtgttgg atttgcgcga cgaccccggc gggcttttga ccggcgcggt cggcgtgtcg
    18961 gcggcgtttc tgccgtctga agcggtcgtc gtcagcacca agggacgcga cggcaaagac
    19021 ggcatggtac tgaaagccgt tcccgaggat tatgtgtacg gtatgggcgg cgaccctttg
    19081 gcgggtattc ctgccgagtt gaaaacgatt ccgatgacgg tattggtcaa ttccggttcg
    19141 gcttccgcgt cggagattgt cgccggcgca ttgcaggacc acaaacgcgc ggtcatcgtc
    19201 ggtacgcaga gcttcggtaa aggttcggtt cagactttga ttcctttgtc caacggcagc
    19261 gcggtcaagt tgacgaccgc cctgtattac acgccgaacg accgttccat tcaggcacag
    19321 gggattgttc ccgatgtcga agtaaaagat aaggaacgta cttttgaaag ccgcgaggcg
    19381 gacctggtcg gacacatcgg caatcccttg ggcggcgagg atgtgaacag tgaaaccctt
    19441 gccgtaccgc ttgaaaaaga tgcggataag cccgctgcaa aagaaaaagg taaaaagaaa
    19501 aaggacgagg atttgtcttc aaggcggatt ccgaaccctg cgaaagacga tcagttgcgt
    19561 aaggctttgg atttggtcaa gtcgcccgag cagtggcaga agtctttggg gctggcggcg
    19621 aaaaagccgg tttcaaataa agataaaaaa gataagaagt aggttttgac gatgccgtct
    19681 gaaagggttt cagacggcat attatgtttg aaaggacagg ttatgagcga ttttttaccg
    19741 gaatttgaac cgtgcggcat ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
    19801 cgcagacagt acaaatagta cggcaaggcg aggtaacgcc ggaccggttt aaatttaatc
    19861 cactatactt gggtagcgat tgggtttcag acggcataca ttcaggattt caccgcctta
    19921 taccgtctga aagacgtttc tgacccatcc ctactacacg ctttgtccgc aaacgtatca
    19981 caggcaagat tatggaagtt atccgatacc caaattcccc cttcaaactc caccaaccct
    20041 tcccgcccgc aggcgaccag cccaccgcca ttgcgggcct gatcgaaggg ctttcagacg
    20101 gccttgccta ccaaaccctg ctcggcgtaa ccggttcggg caaaacctac acgatggcga
    20161 acgtcatcgc gcaaagcggc agacccgcca tcattatggc gcacaacaaa acccttgccg
    20221 cccagcttta tgccgaaatg cgcgagtttt tccccgaaaa cgcagtggaa tatttcgtgt
    20281 cgtattacga ctattaccag cccgaagcct atgtgcccag ccgcgatttg ttcatcgaaa
    20341 aagacagcgc gatcaacgaa cacatcgagc agatgcgcct ttccgccacc aaaaacctga
    20401 tgacgcgcga cgacgtgatt atcgtcgcca ccgtgtccgc catttacggt atcggcgacc
    20461 cgaccgagta tcaacaaatg gtgctgtccg taaaagaagg cgacaccatc gagcagcgcg
    20521 acatcatcgc cacgctcgtt tccatgcagt acgaacgcgg cgatttggac ttcaaacgcg
    20581 gcagcttccg cgtgcgcggc gacgtgattg acgtgtaccc cgccgaaagc tccgaaaacg
    20641 ccttgcgtat cagcctgttt gatgacgaaa tcgaccgcct cgatatgttc gacccgcttt
    20701 caggcagcct gcaccagcgt gtcggacgct acaccgtctt cccgtccagc cactacgtta
    20761 ccccgcgcga caccgtattg cgcgcctgcg aatccatcaa agaagaattg cgcgaacgca
    20821 tcgaattttt cgctcgcgaa caacgccccg tcgagcaaca acgcatcgaa cagcgcaccc
    20881 gtttcgacct cgaaatgctc tacgaaatgg gcttctgcaa aggcattgaa aactactccc
    20941 gccacttctc cggtaaaaaa gaaggcgaac cgccgcccac gctgatggac tacctgcccg
    21001 acaacgccat catgttcatc gacgaaagcc acgttaccgt tacccaaatc ggcggcatgt
    21061 acaaaggcga cgcatcgcgc aagcaaaacc tcgtggacta cggcttccgc ctgccttccg
    21121 cccgcgacaa ccgcccgctc aaattccacg aatttgaaaa agtcatgccg caaaccgtct
    21181 tcgtttccgc cacccccgcg aaatacgaag aagaacacgc cggacaagtc gtcgaacaag
    21241 tcgtccgccc cacagggctg gtcgaccccc aaatcatcat ccgccccgtc gccacccaag
    21301 tcgacgattt aatgagcgaa atcaacgacc gtattcaaaa aggcgaacgt gtgctcgtta
    21361 ccaccctcac caaacgcatg gcggagcaac tcaccgacta ttacagcgaa ctcggcatca
    21421 aagtgcgcta cctgcacagc gacatcgaca ccgtcgagcg cgttgaaatc attagagatt
    21481 tgcggctcgg cctgtttgac gtactcgttg gcatcaacct gttgcgcgaa ggcctcgaca
    21541 tccccgaagt gtccctcgtc gccatcctcg atgccgacaa agaaggcttc ctgcgctccc
    21601 accgcagcct gattcaaacc ataggccgcg ccgcgcgcaa cgtgaacggc gtcgccatcc
    21661 tgtacgccga caaaattacc gactccatga aagccgccgt cgacgaaacc gaacgccgcc
    21721 gcgaaaaaca gattaaattc aacgaagaac acggtatcgt gccgcagcag attaaaaaac
    21781 aggtcaaaga catcatcgac ggcgtgtacc acgaagaaga cagtggcaaa ggccgtagac
    21841 aaggcaaaaa caaggtcaaa gtcggcgaaa tccacaacga agaagacgcg attaaagaaa
    21901 tcgccaaact ggaaaaagcc atgcagcagg cggctaggga tttacagttt gaagaagctg
    21961 ctgtgttgag ggataggatt aggaatatta aagagaattt gttgtttggg gcggagtgat
    22021 tttgaaatag taatcgcagc ctgaatacat agcgtgtgcg ccactggcgc gcgctcattg
    22081 tgttatcagg aatccttgaa atcatgtacg cagcttgcat cacacatcct agctatacat
    22141 aaacaacaag gccgtctgaa ttttcagacg gccttttccg tttacaactt tactacttat
    22201 tcaataacta cacgtgcatt cagaggctgt acggggcggt tgttttccga accgacggcg
    22261 cgggtgaatt tttccgcttg cgcttggtcg atgtggtcat aagttttcaa caccaaccat
    22321 gatacgccct ctgtgcacgg cggcgtggtc agcgaaccgg caaagcggta gtatttcaac
    22381 cgtttcggca gtagggtgga tgcgtcgaac ggttggttga gtttcacttt tcctgcggtc
    22441 atcggcatga cgttccagat ggaagacagg cggccgttgg ttttgccggc ttcatacagc
    22501 acggctaata ctaaaggctg tttgttttcg tctaagtgga cgaagtgagc ttccatcggg
    22561 aaagtgcggc ctttgatttg gttttcgctc ggcacgtgga agtggaactg tttcagggta
    22621 taggtgcggc cgttcacggt cagggtattg ccgccttcgg gataattgac ctgaatggtg
    22681 tggccgttgt tttccacgtc aaccatactc ggtttgtaat tgactttgat ggcgggcagt
    22741 ttgccggaaa cggtttcggt aatgtttacc ggagattggt ttttgccggt ggagcacaaa
    22801 cggaattctt ctgacagatt gccccagctt tcgggagagt cgtgtccggt atagccccaa
    22861 tgggtgtgat tgccgtgtgc ggcggcggag aaagcggtac aggccaacag caggacagcg
    22921 gtcaggcggg gtaaagtgcg ggggaaacga ggcattgtaa gatcctttct attaaggagt
    22981 agaaaaaagg ttgcagtgtg tatgtaaata ttttattttt tattaaatga tatctatatt
    23041 aagtgcttaa gtcaaacttc ccggtctgaa aaccattaga gcaa
//
LOCUS       DAXFWM010000028        23022 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000028 DAXFWM010000000
VERSION     DAXFWM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23022
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.028"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..129
                     /locus_tag="ACFI8B_001046"
     CDS             <1..129
                     /locus_tag="ACFI8B_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3834292.1"
                     /translation="GVGIDVAPGLTLDAGYRYHYWGRLENTRFKTHEASLGMRYRF"
     gene            493..983
                     /locus_tag="ACFI8B_001047"
                     /pseudo
     CDS             493..983
                     /locus_tag="ACFI8B_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1020..1286
                     /locus_tag="ACFI8B_001048"
                     /pseudo
     CDS             1020..1286
                     /locus_tag="ACFI8B_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1788..2411
                     /locus_tag="ACFI8B_001049"
     CDS             1788..2411
                     /locus_tag="ACFI8B_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3834293.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            2523..3905
                     /locus_tag="ACFI8B_001050"
     CDS             2523..3905
                     /locus_tag="ACFI8B_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3834294.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            3909..4433
                     /gene="ssb"
                     /locus_tag="ACFI8B_001051"
     CDS             3909..4433
                     /gene="ssb"
                     /locus_tag="ACFI8B_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3834295.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQNQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            4806..5729
                     /locus_tag="ACFI8B_001052"
     CDS             4806..5729
                     /locus_tag="ACFI8B_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC3834296.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLTLGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRPFKRR"
     gene            complement(5794..7182)
                     /gene="fumC"
                     /locus_tag="ACFI8B_001053"
     CDS             complement(5794..7182)
                     /gene="fumC"
                     /locus_tag="ACFI8B_001053"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC3834297.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7326..9305)
                     /gene="tkt"
                     /locus_tag="ACFI8B_001054"
     CDS             complement(7326..9305)
                     /gene="tkt"
                     /locus_tag="ACFI8B_001054"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC3834298.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            9832..11328
                     /gene="ccoG"
                     /locus_tag="ACFI8B_001055"
     CDS             9832..11328
                     /gene="ccoG"
                     /locus_tag="ACFI8B_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC3834299.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
     gene            11331..11879
                     /locus_tag="ACFI8B_001056"
     CDS             11331..11879
                     /locus_tag="ACFI8B_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC3834300.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(12221..13396)
                     /locus_tag="ACFI8B_001057"
     CDS             complement(12221..13396)
                     /locus_tag="ACFI8B_001057"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC3834301.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEITEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(13554..13670)
                     /locus_tag="ACFI8B_001058"
                     /pseudo
     CDS             complement(13554..13670)
                     /locus_tag="ACFI8B_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(13833..13907)
                     /locus_tag="ACFI8B_001059"
     tRNA            complement(13833..13907)
                     /locus_tag="ACFI8B_001059"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13872..13874),aa:Glu,seq:ttc)
     gene            complement(13925..14001)
                     /locus_tag="ACFI8B_001060"
     tRNA            complement(13925..14001)
                     /locus_tag="ACFI8B_001060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13965..13967),aa:Arg,seq:acg)
     gene            14119..15504
                     /locus_tag="ACFI8B_001061"
                     /pseudo
     CDS             14119..15504
                     /locus_tag="ACFI8B_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
     gene            15614..15802
                     /locus_tag="ACFI8B_001062"
     CDS             15614..15802
                     /locus_tag="ACFI8B_001062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834302.1"
                     /translation="MPSEFFQTAFLPTRFACRVGLPVRPEASDKQGGFYSGLNLNRYG
                     VASPCRTICTVCGFAALS"
     gene            complement(15843..17609)
                     /locus_tag="ACFI8B_001063"
     CDS             complement(15843..17609)
                     /locus_tag="ACFI8B_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFC3834303.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYCLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGQKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(17602..18475)
                     /locus_tag="ACFI8B_001064"
                     /pseudo
     CDS             complement(17602..18475)
                     /locus_tag="ACFI8B_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18512..18796)
                     /locus_tag="ACFI8B_001065"
     CDS             complement(18512..18796)
                     /locus_tag="ACFI8B_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFC3834304.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(18783..19013)
                     /locus_tag="ACFI8B_001066"
     CDS             complement(18783..19013)
                     /locus_tag="ACFI8B_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC3834305.1"
                     /translation="MEKPLNFKPIPYLQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKLGLKEDADGDE"
     gene            19285..19665
                     /locus_tag="ACFI8B_001067"
     CDS             19285..19665
                     /locus_tag="ACFI8B_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3834306.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            19899..20939
                     /locus_tag="ACFI8B_001068"
     CDS             19899..20939
                     /locus_tag="ACFI8B_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFC3834307.1"
                     /translation="MKKIITHRIYPKNENRERVESKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            20936..21310
                     /locus_tag="ACFI8B_001069"
     CDS             20936..21310
                     /locus_tag="ACFI8B_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834308.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            21307..21945
                     /locus_tag="ACFI8B_001070"
     CDS             21307..21945
                     /locus_tag="ACFI8B_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834309.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAECEVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            22065..22352
                     /locus_tag="ACFI8B_001071"
     CDS             22065..22352
                     /locus_tag="ACFI8B_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834310.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            22426..22647
                     /locus_tag="ACFI8B_001072"
     CDS             22426..22647
                     /locus_tag="ACFI8B_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834311.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            22640..22849
                     /locus_tag="ACFI8B_001073"
     CDS             22640..22849
                     /locus_tag="ACFI8B_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFC3834312.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCHEYIQLKTK"
BASE COUNT         5241 a         5682 c         6233 g         5866 t
ORIGIN      
        1 ggcgtgggca tagacgtcgc gcccggtctg accctggacg ccggctaccg ctaccactat
       61 tggggacgcc tggaaaacac ccgcttcaaa acccacgaag cctcgttggg catgcgctac
      121 cgcttctgat tccccgatac cgatgccgtc tgaaccttca gacggcattt ttaatcgccc
      181 gccgtttaca ggcgcggggc gggcgcagta aaatacccga accgccattg ccgatacacc
      241 gtaaccctaa aacccgccat tcccgcgcag gcgggaatcc ggacccccga cgcggcggga
      301 atctatcgga aatgactgaa accccgagat tctagattcc cacttccgtg ggaatgacgg
      361 ttcagcaagc gtaggtcgga tacttgtatc cgacaaaacc tttaacattc ccatcattgc
      421 aatccattgc agcaatgtcc aaaatgtcga attcaagaat ccggcctaca aaatcattcc
      481 gagcataata ctatgaaata ccgtcgtttt taccgcaatg gcggcactta cttttttacg
      541 gttgtaacca ataaacggca gaagattttg accgatgatg cggtgcgttt ggctttacgg
      601 caggcggtaa tggcggtgcg cgaacggtat ccgtttgaaa ttttggcatg ggtgttgatg
      661 cccgatcatc tgcataccat atggcggctg ccggacaatg attctgctta ttcggaacgc
      721 tggcggcaaa tcaagcggca cagccaatat ttaatcggcg gcaatctcag gctttggaaa
      781 aacgcttttg ggaacatact atccgcggtg aggccgattt tgcctgccat tttgattatc
      841 tgcatttcaa tccggtcaaa catggctatg taggacaaat ttccgattgg cggttttcta
      901 cgtttcaccg ttatgtcaaa cagggtattt atccgcataa ttggggtggc ggcaatgcgg
      961 acttttctat tgaatacgat tgaagtaatg tcggattcga gaatccgacc tacggaaaac
     1021 tgaaagagca tcggctgctg gacggcatta tgcgcaaagc ccgccgcaac cgcccgctgc
     1081 cggaggcgca aaccaaacgc aaccgatatt tgtcgaagac ccgttatgtg gtcgaacaaa
     1141 gcttcggtac gctgcaccgt aaattccgct atgcccgggc ggcctatttc ggactgatta
     1201 aagtgagtgc gcaaagccac ctgaaggcga tgtgtttgaa cctgttgaaa gcggccaaca
     1261 ggctaagtgc gcccgctgcc gcctaaaagg cggcccggat gcctgattat cgggtattca
     1321 agggggatta agggggtatt tgggtagagt taggagtgat tggtggcgga aacagccgaa
     1381 aatctgtgtc agggtttcag ctgtcgggag ggaagggaat tttgcaaagg tctcaagata
     1441 tagtggatta aatttaaacc ggtacggcaa ggcgaggcaa cgccgtaccg gtttaaattt
     1501 aatccactat atttccgcct gctgccgccc gaaaagcgta atgcgctgat ggtgctgctg
     1561 cacaatccgg acgacggata accggtgaaa acgggaaatg cccgggcggt gtcgtctgtg
     1621 cctgtttgga caagcgttct tgagggcggt aaaatcaagg tttgcccgaa aagggcaggg
     1681 cgatatgccg cgcttggatt ttgcgcgcgg tttttcgcgt tggaagcaat catgtctgtt
     1741 tgaatattta atgccgtctg aaacgtccgg acgcaccccc gttccttatg aaaaaaccga
     1801 ccgataccct acccgtcaat ctgcaacgcc gccgcctgct gtgtgccgcc ggcgcgctgt
     1861 tgatcagccc gctggcgcac gccggcgcgc aacgtgaaga aacgcttgcc gacgatgtgg
     1921 cttccgtgat gaggagttct gtcggcagcg tcaatccgcc gaggctggtg ttcgacaatc
     1981 cgaaagaggg cgaacgttgg ttgtccgcga tgtcggcacg tttggcaaga ttcgtccccg
     2041 acgaggggga gcggcgcagg ctgctggtca atatccaata cgaaagcagc cgggccggtt
     2101 tggatacgca gattgtgttg gggctgattg aagtggaaag cgcgttccgg cagtatgcga
     2161 taagcggggt cggcgcgcgc ggcctgatgc aggttatgcc gttttggaaa aactacatcg
     2221 gcaaaccggc gcacaacctg ttcgatatcc gtaccaacct gcgttacggc tgcaccatcc
     2281 tgcgccatta ccgaaacctt gaaaagggcg acatcgtccg cgcgcttgcc cgcttcaacg
     2341 gcagcctcgg cagcaataaa tatccgaacg ccgttttggg cgcgtggcgc aaccgctggc
     2401 agtggcgttg attttgaacc cgtgccgcaa ccgaaatacg gcgaatcctg tataatccga
     2461 aaatctgttc actggaagtt cagacggcat tgcaactgtt gatgccgtct gaaaaattat
     2521 ttatggcaag agacaaccgc atccaaatgt ttccgcacga atggcgcgcc agtacgacgc
     2581 tttccggcgt gtacgcgctg cgtatgctgg gtatgttcct cgtgctgccc gttttggcaa
     2641 tatatgccgc ctcgctgccc ggtgcggaag acaataaaac gctggtcggg ctggcaatgg
     2701 gcatttacgg gctgacgcag gcattgctgc aactgccttt gggtatcgct tccgacaagt
     2761 tcgggcgcaa gaaaaccatt tatgtcgggc tggtcgtgtt tgccgccggc agcttccttg
     2821 ccgccgccgc cgatacgctg cccatgctgg ttgccgcgcg cgccatacag ggcgcggggg
     2881 cggtcagtgc tgccgtaacc gcgctgcttg ccgacttgac gcgcgacggc gtgcgtaccc
     2941 gcgcgatggc gatgatcggt ttgagtatcg gtctgacgtt ttcggtcagc ctggtggttg
     3001 cgccgatgat tgccgatgtg gccggcgttc gcggactgtt tatgctgacc ggcattctga
     3061 ccgtcatcag catcggcgta gtggcgtgga tgactcctga tcccgaagtt tccaagctgc
     3121 atgaggacac gcaggcgcag ccttcgcgca taggagaagt tttgaaaaac cgtaggctgc
     3181 tgacgctcga tttcggcatt ttcgccctgc acgccgcgca aatggcattg tttaccgcgc
     3241 tgcctttcgc gatgacccgg ctcggtttgg aaaaaatcca gcattggaaa gtatatctgc
     3301 cctcgaccat tacgggcttg gtggtgatgg ttccgctgat tatcgtcggc gaaacgcgca
     3361 acaagctcaa acaggttttt gttttgggca tcgtctgcat cgcggcggcg cagcttggtt
     3421 tgctgtccgg aatgcactcg atttggctga ttaccgctta tctggttgtt tactttatcg
     3481 gttttaatgt gttggaagcg agcctgccgt ctatggtttc caaaatcgcg ccgtccgacc
     3541 tgaagggtac ggcgatgggc gtgtacaaca cgatgcagtc gctcggactg tttgccggcg
     3601 gcgcggcagg aggcttgctg tttcaaaaat acggctttgc cggcgtgttt gccttttgca
     3661 gcatattgat gctgctgtgg ctggtgattg ccgttttatc gcccgcgccc aagcccgtca
     3721 aaaacctcag ttaccctgtc ggcggcgtgt ggcagggcaa tcgggacggt ttgcaacgcg
     3781 ccctgttgca gttggaaggc gtggaagaca tcggtttcag tttcgacggg cagaccgtct
     3841 atcttaaagt gttgcagaag ggtttcgatc aggctgccgc tgaaaaaatc attacaggag
     3901 tttaaaaaat gtcattgaac aaagtcatcc ttatcggccg cctcggacgc gatcccgaag
     3961 tgcgctatat gcccaacggc gaggcagtgt gtaatttcag cgtcgccacc agcgaaactt
     4021 ggaacgaccg caacggccag cgcgtagagc gcaccgaatg gcacaacatc acgatgtacc
     4081 gcaaactggc ggaaattgcc gggcaatacc tcaaaaaagg cgggctggtt tatctggaag
     4141 gcagaatcca aagccgcaaa taccaaggca aagacggcat cgaacgcacc gcttacgata
     4201 ttgtcgccaa tgaaatgaaa atgttgggcg ggcgcaatga aaacagcggc ggcgcgcctt
     4261 acgatgaagg ttacggtcag aatcaggagg cttaccaacg ccccgctcag caaagtcggc
     4321 agcccgctcc cgacgcgccg tcccatcccc aagaagcacc cgccgcgccg cgccgccaac
     4381 ccgtgccggc cgccgccccg gtcgaggaca ttgacgacga catcccgttc tgaattttac
     4441 ggccggacat cctcgcggag ggaagtcata aaggacggaa aaaccttaaa ccttacgggg
     4501 caaggtttct cttttttgca tttgggccgg cttgttgccg ccccctgcgt tgggcgtaac
     4561 gttgcgattg cgccgttcgg gcgttttgag gcaagcgatg cctgccgcac tcccccgctg
     4621 ttttcagacg gcattttaaa attgaggcgg tgttttcaaa tagtgcgaag caatagtcgg
     4681 gcttcagcca gtttgtttga aatcaaaggg ttggaatcaa acgccaaacg cttgaaccat
     4741 cattgcccga tgcggcaaac cggcatcgtc cggcaatacg gttgtttaaa aatagggaaa
     4801 tcaggatgga aaaccaaagg ccgctcctag gcttcgcgtt ggcacttttg gcggcgatga
     4861 cgtgggggat gctgccgatt gccgtgcggc aggtattgaa gtttgtcgat gcgccgacgc
     4921 tggtgtgggt gcgttttaca gtggcggcgg cggtattgtt tgttttgctg acattgggcg
     4981 ggcggctgcc gaagcggcgg gatttttctt ggcattcatt caggctgctg ctgctcggcg
     5041 tgacgggcat ttcggcaaac tttgtgctga ttgcccaagg gctgcattat atttcgccga
     5101 ccacgacgca ggttttgtgg cagatttcgc cgtttacgat gattgttgtc ggcgtgttgg
     5161 tgtttaaaga ccggatgact gccgcgcaga aaatcggttt ggttttgctg cttgtcggtt
     5221 tgcttatgtt ttttaacgac aaattcggcg agttgtcggg tttgggcgcg tatgcgaagg
     5281 gcgtgttgct gtgtgcggca ggcagtatgg cctgggtgtg ttatgccgtg gcgcaaaagc
     5341 tgctgtcggc gcaattcggt ccgcaacaga ttctgctgtt gatttatgcg gcaagtgccg
     5401 ccgtgttcct gccgtttgcc gaaccggcac acatcggaag tttggacggt acgttggcgt
     5461 gggtttgttt tgtgtattgc tgcttgaata cgttaatcgg ttacggctcg ttcggcgagg
     5521 cgttgaaaca ttgggaggct tccaaagtca gcgcggtaac aaccttgctc cccgtgttta
     5581 ccgtaatatt ttctttgctc gggcattatg tgatgcctga tacttttgcc gcgccggata
     5641 tgaacggttt gggttatgtc ggcgcactgg tcgtggtcgg gggtgcggtt acggcggcgg
     5701 tgggggacag gccgttcaaa cgccgctagt tcgcaggcaa cggaaaatgc cgtctgaacg
     5761 aggcttcaga cggcatttta tttgagggaa ggattagcgc ggatgaacca tatcggcagg
     5821 gacgaccagt tcgtcaaact cttcgcccgt cagcaagccc aacccaacgg cagtttcgcg
     5881 cagcgattta tcgtttttgt aggcggtttt ggcgactttg gcggcgtttt cgtagccgat
     5941 tttgcggttt aacgcagtaa ccagcatcag ggaatggtgc aggaaatagt cgattttttc
     6001 cggtacgggt tcgatgccgg cggcgcagtt ttcgttgaag ctgttgcacg cgtcgcccaa
     6061 gaggcggatg gattgcaaga ggttgtaggc gatgacgggc atatagacgt tcagctcgaa
     6121 attgcccgac gcgcccgcca taccgatggt aacgtcgttg ccgaacactt ggcagcacac
     6181 catcgtcatc gcttcgcatt gggtcgggtt gactttgccc ggcataatgg acgaacccgg
     6241 ttcgttttcg gggattttga tttcgcccaa gccgcagcgc gggccgcttg ccagccagcg
     6301 gatgtcgttg gcgattttgt tcaggcttgc cgccagcgtt ttcaatgcgc ccgaagcggc
     6361 aacggcggca tcgcgtccgc ccaaggcttc aaatttgttc ggcgcgctga caaacggcag
     6421 gccggacagt tcggcgagtt tggcggcggc tatttcagcg tattcgggat ggctgttcaa
     6481 acccgtaccg accgccgttc cacccaaggc gagttcgtac aaacctttga gcgcgtcgtt
     6541 cagacggcct aagctgtggt cgagttggga aacgtagccg gaaaattctt gtcccaaagt
     6601 cagcggcgtc gcatcctgca agtgggtacg gccgattttg acgatggggg cgaaggcttg
     6661 ggcttttttg tccaatgtat cgcgcaaggc ttttaccgcc ggaataaggt ggcggttgat
     6721 ttcaatcgcg gcggcgacgt ggatggcggt cgggaacgcg tcgttggtcg attgcgcgtg
     6781 gttcacatgg tcgttggggt ggacgggccg gtacgccgcc aaacccgtac ctgcaatttc
     6841 gttggcgcgg tttgccagca cttcgttcat gttcatattg gactgcgtac cggagccggt
     6901 ctgccaaacc actaagggga actgcccgtc gagcctgccg ttcagtacat catccgccgc
     6961 ctgcgtaatc aaatccgcct gttcgggctt aatcctgccg agggaaacat tggtcgcggc
     7021 ggcggctttt ttcaccaacg ccaaagcgta aatcagcggc tgcggcaggg tttcgccgcc
     7081 gattttgaaa ttgttgcggc tgcgctgggt ctgcgcgccc caataggctt cggacgggac
     7141 ttcgacattg cccatcgtgt cgtgttcggt gcgggtgttc atacgtttct ccttttgaaa
     7201 tgtgaataag agtgattcgc aaatattata atggagattg gcggaatgag gaagcatcaa
     7261 ggcgggaggg caaaaaaatg ccgtctgaaa agcattcaga cggcatttga tgcggcggct
     7321 gaggtttaca gcacggattt caccgtatca accacattgt ccacggtaaa gccgaatgct
     7381 ttgaacagca gatcggcagg ggcggattcg ccgaagcggt tgatgccgac gactgcgccg
     7441 ttcagtccga catatttgta ccagccgttg gtgtgtccgg cttctacggc gatgcgcggc
     7501 aggccttccg gtaggacggc ggcttgatag gcggcgtctt ggcggtcgaa gacgctggtg
     7561 gacggcatgg aaacgacgcg cacggcaata ccttgtcctg ccaatacttt ttgcgcttct
     7621 acagccaatc ctacctcgga accggtggca atgatgacgg cttgggcgtt gccttgggct
     7681 tcactgatta cgtaagcacc gcgtttgatg tcgttcagtt gttgctcgct gcgtgcttgg
     7741 aatttcaggt tttgacggct gaaaatcagg caggacgggt ggtcttcggc ttttgccgct
     7801 tctgcccaag cgaccagaga ttctgcggtg tcgcacggcc gccatacgtc catattcgga
     7861 atcaggcgca gggtggcggt ttgctcgata ggctggtggg ttgggccgtc ctcgcccaaa
     7921 ccgatggaat cgtgggtaaa tacgaagacg gggttgattt tcatcagtgc cgccatgcgt
     7981 aaggcgttgc gttcgtattc gctgaacatc aggaaagtcg cgccgaaggg ttttacaccg
     8041 ccgtgcaata ccaaaccgtt cataatcgca cccataccga actcgcgcac gccgtagtgg
     8101 atgtagttgc cgcctttgtc gcgggtaacg gagacgctgt ttgaccagtc ggtcaggttg
     8161 gacggggtca ggtcggcaga accgcccacc agttcgggca gctcttttgc caagatttcg
     8221 atgctgtttt ggctggcttt gcgggtggcg acggtttccg ctttggcgca cacttctttc
     8281 aatgccgtct gaacgtactc gtcaaagttt tccggcagct ttttatccat gcggcgcacg
     8341 aattctgcgg cttcggcagg atatttggct tgatattgcg cgaacagttc gttccatccg
     8401 gcttccagtt tcgcgccttt ttctttggca ttccacgcat cgtaaatttc ttgcgggatt
     8461 tcaaacgcag gataagccca acccaaatgt ttgcgcgtgg cttcgatttc gtccgcaccc
     8521 aaaggcgcgc cgtgggtttt gtggctgcct tctttgttgg cgctgccttt gccgattaag
     8581 gttttgcagc agatgatgga cggcttgccg gtttcggcgc gggcggcttc gatggccgtc
     8641 tgaatggcgg cggtgtcgtg gccgtttacg ttgggaacga cgtgccagcc gtagctttca
     8701 aagcgttgcg ggatgttttc ggtaaaccag ccgtccactt tgccgtcgat ggagatattg
     8761 ttatcgtcat acaaaacaat cagtttgccc aagcccaagg tgccggcgag tgagcaggct
     8821 tcgtgcgata cgccttccat caggcagccg tcgcccataa agacgtaagt gtagtgatcg
     8881 acgatgttca aaccgtcttt attaaattcg gcggcaagga ttttttctgc caatgccatg
     8941 cccactgcgt tggcaatacc ttgccccaac gggccggtcg tggtttccac gccgtcggtg
     9001 tagccgtatt cgggatggcc gggggttttg ctgtgcagct ggcggaagtt tttcagatct
     9061 tcaatgctca ggttgtagcc ggtcaggtgc agcaggctgt acaacagcat agacgcatga
     9121 ccgttggaga ggacgaagcg gtcgcggttg tagaatttgg gattggcggg attgtgattg
     9181 aggaatttcg tccacaatgt ttccgccatt tccgccatac ccataggcgc gccggggtgg
     9241 ccggaattgg ctttttgaac ggcatcggcc gagaggaagc ggattacgtt tgccagttga
     9301 gacattttgt attttccttg ctggtgtttc ggataagtgg ataatcggaa ggcgttgatt
     9361 atcgcccgat tcgcttatgc tttcaagaaa agggcggacg cgggggaagg cggcggcagg
     9421 gggtggggaa ggattttcga tttgcgggcg aagcctgcca ttattccttt tgaaataaaa
     9481 cgttatagat tgtgtgccgg attgttgata gcgtttgttt atgagcttgc gccggcggtt
     9541 ctgccgataa gggggtgtct gtttttttag tctttgtttt ttaagagtat tttaattttg
     9601 ttcgggcggt gcggtaaaat cagggcgttt tgatgcggat gggaaatgta ttcgcccaat
     9661 acatccggac ggcgcataaa gttgtacaat agcgggaata tattttggga aaagcggatt
     9721 ttctcaaaac ttgaaacaca atgccttaaa aaaacaagaa aaatgacgtc tgaaaagcaa
     9781 aacggcggcg gtaacgggca aaccatcaat caaaaatcta aggaatgcag aatgaccacg
     9841 gaaaaccaag ccggcagtcc ggcatccgga atcggcacgt ccgaacaaac aaaagccgcg
     9901 ccgaaagtga aaaaaacgtt cgatccgcgt gccagcgtga ttcaaatcca tcccgaaggc
     9961 gaacgcatcc atcccaaaaa ggcggaagga cggttcgcca aactgcgtat cgccgccgta
    10021 ttggcgaccc agtttgtgtt ttacgtcatt ccgtggttca actggagcgg aaggcaggcc
    10081 gtcgttttca atatccccga acggcatttc ttcattttcg gattgtcgtt gggggtgggc
    10141 gatttgattt accttgccct gctgctgatg atttgcgcct tcgggctgtt ttggtggacg
    10201 acgattgccg gccgtttgtg gtgcggctat tcctgcccgc aaacggttta caccgaaatt
    10261 atgctgtgga tcgacaacct ggtcgaaggc gatagaaaca aacgcctgaa gctggaaaaa
    10321 tcgccgtgga atttcaccaa aatccgcatc aaagccacca aatacctgtt gattttcctt
    10381 gtctgcgcgt ggacgggcat cacgtttgcc ggctggtttg tccccatccg ccagtttgtt
    10441 cccgatttat tcaccggagc agcaggcggc ggcgcgatgt ttgccgcagc gttttacggc
    10501 tttatgacct tcttcttcgc ccatattatg cgcgaaaaag tgtgcctgca tatgtgtccg
    10561 tacgcacgtt tccaaagcgc gatgttcgat aaggacacgc tgattgtttc ttatgacgcg
    10621 gagcgcggcg aaccgcgcgg cgcccgcaag aaaacggtca acaaggaaga ggcgggtttg
    10681 ggcgactgca tcaactgtgc gatgtgcgtc caagtctgcc ccgtcggcat cgacatccgc
    10741 aacggcctgc aataccaatg tatcggctgc gccgcctgta tcgacgcgtg cgatgagatt
    10801 atggacaaaa tgggttatcc gagcggtttg atacgctata cgaccgaaag cgcgctggaa
    10861 cacgaatatg ccgaaaaaga cattaaaaaa cggctgctca gaccgcgcgt ggcaggttac
    10921 ggcgcggtgt tggcagtggt tgtcgccgcc ttcctggtcg gtttgtccac gcgcaaaatg
    10981 gtcgaagtcg atattctgaa agaccgtggc gtaatggtgc gcgaaaacgc caaaggttgg
    11041 ctggaaaacg catatagcct gcgtatcatc aacaacagcg aaaaagaaca gctgattacc
    11101 gcaagtgtca aaggatttga cgaaatcgcc ctgactgggc tgcctgaagg cggtataaaa
    11161 gtggctccgc gcgaaacaat aacccttccc gtccaagtgt ccaccattcc ggaatacgcg
    11221 gacaaaggca gtcatcctat cgagtttatc ttccaatacc gggaaagcgg tgcatcagac
    11281 ggcaagccgg ttgttttgga agaagacgca acctttatcg gagaataacc gtgtctcaaa
    11341 acaatccaat caagccttgg tacaaacacg tctggccgtg ggtcttgatg gcggggccga
    11401 tttttgtcgt catcgccagc gtcgctatgt tttttgtcgc gcagcagcac gcgacagatt
    11461 tggttacgga cgattattat aaggatggca agcatatcga catccagctt catcgggatg
    11521 aagaagccgt cagacggcat atcggggtgc aggtcctcat ttctcccgat atgaatgcgg
    11581 caaaagtgtt tgtcggcggc gagtttgacg gcaaacagcc tttgaacctg ctgctgatgc
    11641 acccgacccg caaggcggac gatcaaaccg tcgccctcaa gcccgtcggc agcgcgcaga
    11701 acggcagggc ggaatatgag gcggtgttca aaacccttcc gccggccaac cactggtatg
    11761 tgcgcgtgga ggacgcggca ggcgtgtggc gcgtcgagaa caaatggatt accagccagg
    11821 gcaatgcggt cgatttgacc ccgatggaca aacttttcaa taatgcagga agcaaataaa
    11881 aaagccgtgt tttcattgca ttggtttgtt atatgcaatg tcgggcaggc attgtttgat
    11941 tattcggcgt gatggtctga tttgtcggac gaaaataaat gtatctcatc ccgtttattt
    12001 tttaacatca ttccgaaagg cagcctgaaa aatgccgtct gaaaggcttc agacggcatt
    12061 tgtgttgtat gtccgacata atgcggaagg cattttgttc atataaaaac gtttttattt
    12121 ctctccgttt tttcgacact gttccggaac acaacctgaa aaacgccgtc tgaaagccct
    12181 tcggacggca ttttgtttgc aaatcaaatc ctacctgatg tcagcgtccg agcgttgcaa
    12241 caggttcgct ccccgtcagc agatattgta tcgtttcgcg gacggtgcgg atggcattac
    12301 cgaagggaag ctggtctttt cccctgaaaa acaacccctt atcaacttct ccacggaatg
    12361 cggcagcaag ctggatatca atacagaatt gccctgcttt ggaaagcccg tcgcgcagac
    12421 cgcagctggt caggcagttt aaaccttggg tacagcggcg cgggtcggct ttggcgtttg
    12481 cctgaagttt gccttcgcgc ctgatatagc tgtctaggaa tttggtgcgg ataccgcgcg
    12541 ccggcaaacc ggcaacagac ataaattcga ctactttttc agtttccgca ctggcgagcg
    12601 tttttttaaa gttgaggtgt gcatctcctt cttcggtaac ggcaaaagcc gtaccgattt
    12661 gaacggcgga ggctccccag ttctttaggg cggttttgac tttttcaaaa tttgccatgc
    12721 ctcccgcaag aataagcggg attttttctc cctccagccc caaattttta aacacttcaa
    12781 acgtttcctc aatcacgcgt ttgaagtcga atttggcatc gtttacgcct tcaacggttg
    12841 atgcacccaa atgtccggcc gcatgggcag gatgttcgac tacaatcgca tcgggcaata
    12901 tgcctttttt catccaacgt ttcaagacga tattaatacc gcgcgattcg gacagaatcg
    12961 gcagcagcgc gacatcctta tgatagccct cggttatttc cggcaggtct aaaggcaggc
    13021 cggcacccat cacgaccgca tccgcccctg attcgcaagc ctggcggaca tacgcggcgt
    13081 ggtctttgac cgccttcatc acgttgaccg caatcagtcc ttttccctct gaagcacttt
    13141 tggctttttg gatttccctg tctaatgcgg tacagttcaa agacgtatat ttctcttcac
    13201 tcggattgat ttgggattcg gcgagcaggt cttcgtgaag gtggcgcaaa tctacactgg
    13261 caatcgttcc gatgccgttt tcacgcgcca ccgcgctgga taaacccgat gcggaaacac
    13321 cgacccccat accgccttgc acgatgggaa taagggattt tccacgaata atcaaagggt
    13381 caaaaatatt ctgcatcagt ttctccgaat atacgaatca gtcaggctca tgaaaaatgg
    13441 ttttaattgt tttagaacca ttactcaaat ttggtattat actctaaata ttgcagtggt
    13501 aaacagaaaa gcagaaggag ctttggaata tgctgttata tctgtaccaa tcggacaaaa
    13561 tatagtggat taaatttaaa ccggtacggc gttgcctcgc cttgccgtac tatttgtact
    13621 gtctggggct tcgtcgcatt gtcctgattt aaatttaatc cactatattg aacggacacc
    13681 gcatccggtt atgggatatc ggtttgcatg gcaaaagttt gaatatgatt cgattacgtc
    13741 tgagattctg atgaagtggg taggtaaaga tatggtaacg aattgccgag gcaataaaaa
    13801 tccccgaggg attatttcgg ggatttggaa tctggcacgc ccacggggaa tcgaaccccg
    13861 gttaccgccg tgaaagggcg atgtcctaac cgctagacga tgggcgcgta ttgtattttc
    13921 ttactggcgc acccggagcg attcgaacgc ccgaccctct ggttcgtagc cagatactct
    13981 atccaactga gctacgggtg cgctgcaata aatttttgaa ttttatggta atcatcggat
    14041 tctgtcaatt attttgttgg atgaaatgcc ttaaaagtgt aaaatatcgg tttgttgttt
    14101 gaatttaaag agaataaaat gattgttgct tttcgctggc ccttgctgga aaaggcgttt
    14161 ttacggtttg gaatgcctgt tgccgtggtt gatttggaat cgacgggcgg caatctgtat
    14221 gaagacaggg taaccgaagt ggctttggtc aagtttgggc agggaagggc ggagaggtat
    14281 gaatggctgg tcaatcctca aaaaccgatt ccgcagtttg tggcggaact gacggggatt
    14341 tcagacggca tggttgccga cgcgccggtg tttgctgaaa ttgccggcgg gttgttttcg
    14401 gtattgaagg ggtgcgtgct ggtggcgcac aacagccgtt tcgactatac gtttttaaag
    14461 catgagtttc atcgtgcggg tatcggattt tcatcgcctg ctttgtgcag tgtgcagctt
    14521 tcgcgtcggc tgtatccgca gttttacaag cacagcctgg acggtatcat cgaaaggttg
    14581 gggattgttg tggaagacag gcatcgtgcg atggcggatg tatcggcgtt gtgtgattat
    14641 ttggaataca gcctgtcggc acacgggctt gaggagtgga gcaggcagtg tttccgtttg
    14701 acgaatccga aacttcttcc tgccgcgctt cccgaacggt tgagggaaca gctgtacggt
    14761 ttgcccgacg gtacaggtgt gcttgcctgt ttcgacggcg ggggaaaagt aaattatatc
    14821 ggtacgtttg aacgggcata tggcgaggtt gcggctttgt tggattccgg aaaagccccc
    14881 gtttgattgg tgcaatacgg aggaagtccg tttttttccc gcattgggca gcctgcatgc
    14941 atataagatt aaagcggaat tggtcggacg ttatcattcg ggttgttatg tatctgccaa
    15001 aaatctgctt aaaacattta cgaccgtcag gtttgaaaaa ggttcagacg gcatgttgaa
    15061 tgcgaaaaca gcggctttga aaaacggtgt gacggataat ccgcctaccg gattatttgc
    15121 caataaaaag gcggcgagac gggctttgtc gtcgtgggcg gaaacatacg gattgtgtcc
    15181 tgcctcagcc ggtatccttc cggacggtta tgcagaggat gagccttgtc ccgtatatgt
    15241 ttcaggccga tgcgataagg cgtgcggccg ttttgacgaa caagttttgg cgtttgcaca
    15301 caagttgcct gttttggatt ggggaaaaat gcatgaggtg gaaattaccg aaaccgatcc
    15361 tttaacgggg gaaaagtcgt tctgcatgga atgggcggcg cattggaaat ggatgacggg
    15421 ctttggtatt tcgataaaga tttaccggat gtgtttaagg cgaagtttaa aacggacagg
    15481 aaaaatatta aggaaatcgg ttaaatcgat accggttggt gaaagctggt aagtatgtaa
    15541 acagccgttc gtattgattg cctgatcggt ctggattgga atgtgttgcc gtttgcaggt
    15601 aatgtgtctg aagatgccgt ctgaattttt tcagacggca tttttgccga cgcggtttgc
    15661 gtgccgtgtc ggtttgcccg tccgtcccga ggcatcggac aagcagggtg gtttttatag
    15721 tggattaaat ttaaaccggt acggcgttgc ctcgccttgc cgtactattt gtactgtctg
    15781 cggcttcgcc gccttgtcct gatttttgtt aatccactat aaaagctgtt ttcggttgaa
    15841 acctaaccgg ttttcaaaag tttcagacgg catcttgtcc gggtgcgttc aaacgcctct
    15901 tccagcctgc cctcggtcac accttccggg tagtgtttga ggatgaccga cactgcctgt
    15961 ttccagtcgc cgccgtctgc ctcgacgcgg ggttttaagc gtttggcgat ttcgaccttg
    16021 ctcaatacct gctcggcgac ctccatcttg ttgtaatcca tctgttgccc ctgtttgggc
    16081 ataaagagcg tattgcttgc ggcaagcgta tcttcttgat gtttgtacgg gtcgattttg
    16141 ccgccgaacg ggactgcctt gcctttgcgt ttggcggctg ccgcctccag cgtttccgca
    16201 cccatcgcca gcttgtccag ctctttgcga tgccgttgcg cgtctgtatc ggccggggct
    16261 ttgtattccg ccccgatgac cgcggcatca gccttgaagc ccatctcgtc aaaaactact
    16321 tcgggtacgg atacccaaac ctcgttaccc tccgcgtcat aagtggcgac ccgcgccccg
    16381 tttgcctccc aaggattctt accgaccaaa actttctgac cgaccaaaat ccccttgatg
    16441 ccttttacgc tatatacccg tccgccaaag cggatttcca aatccgccga gactttcgcc
    16501 tctttcggcg cgctgacggc aagctctcgg caatattcgg caggcggcgg caggatgagc
    16561 tgctcgggtt tgattttgtt ccacgcctga tagcgggtca tgccgtggcg gctgtgtttt
    16621 tgcgtaccgt tgtagtaacg catccagcgt tccgataaag cattgagctg gtcgatgtcg
    16681 tgtacctcgg taaagcgcaa cccgctctca aatgccgttt ccacaatatc gttggctttt
    16741 tccacttgcc ctttggcacg cggattgccc ggcttgttga tttgcacatg cacatccaag
    16801 ggcttgcaca aatttttaaa cgccgccgaa gtattcgcgc tgcccgggtc aagcatgacc
    16861 atgcgcggta cggtccggaa cgggtctttg ccgatgtctt ttttcgcctg catcatgtag
    16921 atgaaaaaat cacagaggtt cgcgctggtt tcgccgccga agtaataacg caccgaaatc
    16981 gtgccggagg catggtctgt ccccgtgtac cgccaaacgc ggtcgttttc gattttgacg
    17041 acgtttttcg gcttgttttt ataaaactcc tcttccttca tcacccgcag ccccgtatcc
    17101 ttgccctgac gcggcaggca atacaaaacg cacaaactcg ggtcgatttg ccaacaatgg
    17161 ttcgggtgtt ccgatttcat gcggctgacg gggtcgggct gcaaaagctg gtcggaatgc
    17221 agcttgtatt cccgtaaagc ccgggtaatg gtgttttcag aaagggggat gacttccccg
    17281 gtttccccgt caatccgcgc cgcctcgatt ttcccgttgg cgcgcagcat ttccaccgcc
    17341 tgccgcaccg gcatcaaccg cttgccgttg cgcctcatcg cctccgccaa aaccgccgaa
    17401 atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg ccgtttgcgc
    17461 gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct gatgcccaat
    17521 tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gccccattgc ttccgcctga
    17581 ttttcgactg ctttaagacg ctcaatcatt gccggattca tcgccttctc ccgtttcacc
    17641 gcccaaccat tccggcacat tgtctgccgg tgcttcggtc ggtagggcat agctttcgcg
    17701 cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatcttcg cgcggtggtc
    17761 aaatccatgc gcctcgccgt gcgccgccat ctgctcgaac atatcgcgca atcggctgat
    17821 ttgcgagcgg ataccgactt ccaagctgcc aagctgcatc gtcagctcga tgcccacatc
    17881 cgcaggttta ggctctttga cacccgtctg ctttttcgac agcttttccg ccagctcatc
    17941 gaccttttta tttttatcgg caatcacctt atctttcgct tccgccgttt cacggctttc
    18001 gcgcagggcg acgcgcagct ccttaatagt catacggtcg acatcgtcaa aagtgttgcc
    18061 gttgacctca ccgccttcgg caagctccaa caaagtgtcg tcgtcttcga ccagcagctc
    18121 gagcagtttg gatttgccca acgtcatcag cttaggctgc gcttgtttca ttttcgggtc
    18181 gataaaacgg agggtggcat tcatcagtct ttgggattcc cgccgtccga ggccaactct
    18241 ttttcagcga tttcggcaaa ccgcccatgc ggcgtatgct ctttgatgat gatcagcgcg
    18301 cgtcccggct caaacatccc ttccatcgtt tggcgtccgc aaatcgtccg cgctcaatcc
    18361 ataccgtctc gttgtaggcc tcgccgcctg aaaacttgtc cataacctcc atactgtgga
    18421 tagccaattc gtttgccgtt acgccgaccg tatgtcctaa aatttctgtt gccatttctt
    18481 ttgctcctca aatgcgacga cgtcgtcgca tttagtaaat tcggttttcc aattcttgca
    18541 agcgcgcatt cagccgctct tgctgttttc tgaaccgctc tgcgatttgt agggttttga
    18601 tgccgtaggc gtagttgccg ttttcaagct tgatgaccaa tcccgatgca accaaatcat
    18661 caatatccct gctgacttgt gatggcgtca gccccaggcc gtctgaaagc tccttattgc
    18721 tgatgccgat taccggatgc gcgtccatcg ccaaaaacac ccgcaacagc cgctgcgcct
    18781 ttttactcgt cgccatccgc atcctccttc agcccaagtt ttaccgccgc ttcgtggctt
    18841 ttgccgaaat tgcctttcag cttgccgcgc aaggggtgtt ccaccgcggt gcgctccaaa
    18901 ttgaaatgtt tcgcccggtg cgtcttgcac acgccgttgc gcttaaacca cacggccgtg
    18961 ctgccgcgcg tttgcagata gggaataggc ttgaaattta aaggtttttc aatattattt
    19021 atgcctttcg tgtggtataa tgtgtttagt tttaggcctc tttgcacgat aaacatccgg
    19081 tctgttgtgc agcagcaggg tcttggcttg ttttaaaata aacaagcttt ttcccgtagc
    19141 cgggtgtatg tgatccatca tgagctgtcg cgcatgagcc ttcaaagtgt tcattttttc
    19201 gtcctttttc gtgatgattt agggtgtttc atgtcttgat gtgtgaatta tgagaaaaat
    19261 atttctcttt tcaaggaaat ttaagtggaa aaaacttctc tttttggtaa gcgattgaaa
    19321 gaagaaagaa ttaaattagg cttgaaccaa gcagaagctg ctgaaaaatg tggtttttct
    19381 cgtgagatgt ggggaaagtg ggagcgtggt gaaaatcgcc catcaagtga aaaattattc
    19441 tctttttcta aaatcggcat cgatattgat tacgtcatgc acggcagacg cggcgaaaca
    19501 gccgccatgc cgtctgaatc cttgagtgct gaagaaaaag aactgcttgc cctgttccgt
    19561 cagctcggca gcggcagccg caaagagctt gctgactacg ccgcctttaa gctggtggta
    19621 gagaaaaaag cccaaactgc gcttggtaaa gtgagcaacg gataaaaaat gccgtctgaa
    19681 gatttcagac ggcatttggg acactctgac agtcgggctg ctccggtgtt ctgaaacaga
    19741 tattaagcaa taggagcggc cgtgcatttt aaagcgtatt aaaaaccacc ccgatattca
    19801 ggtcatcggc caagtggttc agatttcaaa agaccttaat taacttacaa taggaaaaat
    19861 gatgacagaa acgaattgct cttttgaagg gctggtaatt gaaaaaaatt attacccatc
    19921 gtatttatcc gaaaaatgaa aatagagagc gtgtagaatc taaaatcagt acacatttga
    19981 ttgatttgcc tattacggca agaagaacat tggaaaccag gctgaccaaa gctttgggta
    20041 ataaatctca tggcattgaa atgtctgttg tcaatacggc tgaaaatagt ttttttcaga
    20101 tagcggcagc catacaactc aaagaggaag cagagtttat tgaggattcc gcacaatttg
    20161 cccacatgtt gaccgatgca caactgaata caaatgcacc gggtggtatt ttgttggttt
    20221 taaaaggtag ggttggagat accggtaagc cgtttttatg tgtaattaag gctgaacctc
    20281 aagatgggtt ccgaaccaaa gaagaggatg actttatcac gattgaattc ttagaagaat
    20341 tattactgac cgattcagca agattattca agataggttt tttggtggct gaaacagtaa
    20401 ggccgctaga gcaaatacaa tctgggaatt atcgagcttt tttgtatgac catctgatga
    20461 cacaaacgga aactagaccg gcagcttcct atttctatca agtattcttg ggtatgagta
    20521 tagctgcttc ttcccgtaaa ttgacgcagg atttttttga gtggacacgc aattttatcg
    20581 ataactctga tttaagtgat gatgcaaaat tagatgcgca tgaagcattg cgcgttacat
    20641 tgaaaagtgc ggaagcaacc attagtgtaa ataattttgc ccaaaatcat ttacctcaag
    20701 aaaaacgaac aacttataca gaatttatgg tggaaaagga ttttcctcaa aatgccgtaa
    20761 gtaaagatat tgaatatatt aaaactcgtt tacgcaaacg gaggtcttac ggatttagta
    20821 acggtgtagt tatcttgact cctcccgagc atactcagga ctatatggaa attgcgccaa
    20881 cggaagatgg ggaatatact gttgtcctaa ttaaaggaca gttacaacaa caaaaatgag
    20941 tgatattgat gatttcaaaa gctatttaga aagccaccaa gcggcatttt ccgcttgggg
    21001 tagatttgtg gctgaagaaa ttcaaaatca attatccaat gtcatttctc ctgttccggt
    21061 tgccaatttt ctaaaaattg aagcaaagcc tggagtcaaa gagatttctt cagctctagc
    21121 caagattggc cgaaaaaatt acacttcacc tcaaactcaa atgactgact tggtcggcgt
    21181 acgttttgtg gcattgcttg cggaacatat tcaaatagtt tgtgaaatca ttgaatcctc
    21241 atctcagtgg aatgctaaag tttcgaaaga ttttgcagat gaaattcagc aaaatcccaa
    21301 agcaatttga ctatacaatc caaacattac gagattcgtc ctaaacaagc attcattaca
    21361 cccgaaaatg tcagcatccc tgctgatttg tgctgtgaag ttcaggtgcg ttctttactc
    21421 caacatgctt atgcagagct ggttcatgac aatatataca agcctgacgg caatgttcca
    21481 aagcaagcag agtgcgaagt cgcgaagagc atggctttga tggaaactac agatgactta
    21541 tttagtcgta ctctggcaat tttgaaagaa gcaaatcaac cacaagaaga gcttcttccc
    21601 caattatcac agctttatca aaaggaaata gggcttgttc ctgaagttga taaaaaaacc
    21661 aatatgattt ttctagaaac atttcaatcc agcatttccc aatcaagtat tctttctgac
    21721 atccgttcac ttttgaatga gaagaaatat attgccaagc ggataaaaga aaatgctgaa
    21781 gaaatgtatt ttttcagtca gccagcagct ttgttggtct attggctgat tgagaaagtt
    21841 ggtgcagatg aagtatggaa aaaatggcct ttgcctgcat acaacaaaaa cctgaaattc
    21901 atttgtactg atttggataa gcaaccatcc catgaactct tttaaccccc tttaaaagcc
    21961 cattcagacg gcctttccta attatgcctt aatcaaacgc ggcatagcgg cagttccgca
    22021 gctggcggaa atcaaaaaat ccgcaaatca ggaagggggc ggcaatgatt gaatttgtcc
    22081 gagccaaaaa acggctgctt tgggcatttg tacttttgct tgcgtgggcg tgcggctacc
    22141 ggtacgctgc cgacaaggcc gaagcggaac aaaccgccct gattgccgcc tatcggcatt
    22201 cttctatggt tgcggcggaa caatacgcct tgcagcttaa aaaagcgcag gacgaaaggc
    22261 agcggtggta cgacttttcc caaaaacaaa gcacggattt ggcggccgcc ctgagcgaat
    22321 tggacaaaac gcgcaacacc ttacaggagt aaacccgtga agcggcagac aaagacggca
    22381 accgttttaa cggccttggc acgaacagcc tgcacctcta caaccgtgcc ttcggatacc
    22441 ccgattaaaa ccgttgccgt cgcggaaatt ccgcccgttc cttccggact gctggtcgaa
    22501 tacgaacgcc ccgagcgtcc ggccggcggc tctcccgaac aacttttaaa ccacgccgtc
    22561 cgctatggcg gatactaccg aaagctcgaa atccaaattg agggctggca gaactggcac
    22621 acgaaaggcc gtctgaaaca tgactgattt tgccgaccgc gcatccgaac gcgaagccga
    22681 atttctggtg gaagccttgg cgaaacacca accgccgtct gaaaacaccg ccggcttcag
    22741 ccattgcgaa gactgcggcg acccgatacc ggaagcaaag cgaaaagccg tccgaggctg
    22801 tacgcgctgt gttgtctgcc acgaatatat tcaattaaaa acaaaatagt acaacactcg
    22861 acgttgaagg tttaaccatg gcatactctg cggacttaag aaacaaagct ttaaaccata
    22921 gcggattaac aaaaatcagg acaaggcggc gggccgcagg cagtacgaat ggtacggaac
    22981 cgatccgccc ggcgctccat caccttaggg aatcgtcctc tt
//
LOCUS       DAXFWM010000029        22962 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000029 DAXFWM010000000
VERSION     DAXFWM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22962
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.029"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..114
                     /locus_tag="ACFI8B_001074"
                     /pseudo
     CDS             1..114
                     /locus_tag="ACFI8B_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153708080.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(156..326)
                     /locus_tag="ACFI8B_001075"
     CDS             complement(156..326)
                     /locus_tag="ACFI8B_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC3834313.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(459..1541)
                     /locus_tag="ACFI8B_001076"
     CDS             complement(459..1541)
                     /locus_tag="ACFI8B_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC3834314.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRVVKEKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVEGGYTVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1677..2225)
                     /locus_tag="ACFI8B_001077"
     CDS             complement(1677..2225)
                     /locus_tag="ACFI8B_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC3834315.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVLKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2365..2736)
                     /locus_tag="ACFI8B_001078"
     CDS             complement(2365..2736)
                     /locus_tag="ACFI8B_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC3834316.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2921..4612)
                     /gene="dld"
                     /locus_tag="ACFI8B_001079"
     CDS             complement(2921..4612)
                     /gene="dld"
                     /locus_tag="ACFI8B_001079"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC3834317.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDMPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4709..5128)
                     /locus_tag="ACFI8B_001080"
     CDS             complement(4709..5128)
                     /locus_tag="ACFI8B_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834318.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5351..5447)
                     /gene="ffs"
                     /locus_tag="ACFI8B_001081"
     ncRNA           complement(5351..5447)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFI8B_001081"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5708..9541
                     /locus_tag="ACFI8B_001082"
     CDS             5708..9541
                     /locus_tag="ACFI8B_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC3834319.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKGVELFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9618..10619
                     /locus_tag="ACFI8B_001083"
     CDS             9618..10619
                     /locus_tag="ACFI8B_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC3834320.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(10680..10862)
                     /locus_tag="ACFI8B_001084"
     CDS             complement(10680..10862)
                     /locus_tag="ACFI8B_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834321.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(11035..11166)
                     /locus_tag="ACFI8B_001085"
     CDS             complement(11035..11166)
                     /locus_tag="ACFI8B_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834322.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
     gene            complement(11425..12569)
                     /locus_tag="ACFI8B_001086"
                     /pseudo
     CDS             complement(11425..12569)
                     /locus_tag="ACFI8B_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12894..13370)
                     /gene="greA"
                     /locus_tag="ACFI8B_001087"
     CDS             complement(12894..13370)
                     /gene="greA"
                     /locus_tag="ACFI8B_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC3834323.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYGIIEVRYI"
     gene            complement(13635..13763)
                     /locus_tag="ACFI8B_001088"
     CDS             complement(13635..13763)
                     /locus_tag="ACFI8B_001088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834324.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            13762..15063
                     /gene="aroA"
                     /locus_tag="ACFI8B_001089"
     CDS             13762..15063
                     /gene="aroA"
                     /locus_tag="ACFI8B_001089"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC3834325.1"
                     /translation="MTESVRLPAASPKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHVGEREDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(15141..15674)
                     /locus_tag="ACFI8B_001090"
     CDS             complement(15141..15674)
                     /locus_tag="ACFI8B_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC3834326.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(15731..17257)
                     /locus_tag="ACFI8B_001091"
     CDS             complement(15731..17257)
                     /locus_tag="ACFI8B_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC3834327.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(17369..18910)
                     /locus_tag="ACFI8B_001092"
     CDS             complement(17369..18910)
                     /locus_tag="ACFI8B_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFC3834328.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFVLGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            19058..19837
                     /locus_tag="ACFI8B_001093"
     CDS             19058..19837
                     /locus_tag="ACFI8B_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC3834329.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            19834..20535
                     /locus_tag="ACFI8B_001094"
     CDS             19834..20535
                     /locus_tag="ACFI8B_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC3834330.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            20532..21986
                     /locus_tag="ACFI8B_001095"
     CDS             20532..21986
                     /locus_tag="ACFI8B_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC3834331.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIVITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(22135..22554)
                     /gene="fitB"
                     /locus_tag="ACFI8B_001096"
     CDS             complement(22135..22554)
                     /gene="fitB"
                     /locus_tag="ACFI8B_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFC3834332.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(22554..22790)
                     /gene="fitA"
                     /locus_tag="ACFI8B_001097"
     CDS             complement(22554..22790)
                     /gene="fitA"
                     /locus_tag="ACFI8B_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFC3834333.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         5308 a         6141 c         6249 g         5264 t
ORIGIN      
        1 gtttgtccgg atgaaacagg attcgaccgc cgcctgttcc gcccctatgc ccgcagcctg
       61 aaagggcaaa tggcgaaagc gcggataagt gggaaaagat accggcgctt atctgcggaa
      121 cacgccgagg gattttccaa ggggtgcggc tgccgctata tcacgaaatc caacaggtag
      181 aaatcttctt tgcccacgcc gcattcgggg catttccagt cgtcggggat gtcttcaaac
      241 tttgtcccgg gggcgatgcc gtgttccggg tcgccgagtt cttcatcgta aatccagccg
      301 caggggccgc acatatattg agccatttgt gtttccttgt ttgtaccgtg cttttttgca
      361 gcgggccgcc ggggtttggt tcggatgccg tctgaagcgt gccgccgcat ctgtcccgcc
      421 ttgggcggtt tgtgcttcag acggcatttt ccgcctgttt attttgcggc ttcgattgct
      481 gctatttctt tgcagaggtg tttgattgcg ggggttacga tggcgacaaa catcgcttcg
      541 ccgacgcgcc tttgggcggg actccgcatc aggtagcctt tcgcgccgga atgcagggcg
      601 gcggattgcg cagcggcaag ggccagtacg gcggcggctt cgcgcagctt gagggtggcg
      661 aggttgtcgg gcgtgttgtc ccacgccaat ccggcgagcc gttcggtttc tgcccacgcg
      721 ccgtccagcc ttgctttgag gctgtcgtag ccgtcgtcga gatagctgtt gacttctgca
      781 ttgacgatgt tggcgaggcg gatgatgccg aggctgccgt ctatcacgcc cgcgccgatg
      841 ccgatttgca ggaggataaa gcctgctttg atgctttgga tgtagccggc aaactgttcg
      901 ggcgcggcga tgatgtcttc gtcggggata aatacgtctt tgaagttcag gctgaaggtg
      961 cgcgtgcctt cgagggcgca aaattcgggg cagttttgca ggcttacgcc ttccctttgc
     1021 ccgcctgtga tgaacatgac gtagccgccg ccgatttggg cggtattcgc ccaaatgtgg
     1081 tcttcgccga tgttggacac ccacggcagc gcaccgttga ctgtataacc gccctccacg
     1141 cactcggctt gaaggttgtg tttttcaatg ccggcaaggt gtttgacggt attggacatt
     1201 ccggtgcccg ccaatacttt gccttgcagg atgtcggcga ggtatttttc tttgacgacc
     1261 cggttgggcg tttggtgcag ataccacgcg caagccgcct gacaccacgc gctgaaggag
     1321 gtcgcgccgc attctttgcc gatttcgcgc aagacggcga ttcgtgttgc caagcctaag
     1381 ccgttgccgc cttcggcttc tataccgact gcgccgattg cgccgagttc gcgcataaat
     1441 gcttcggggt agtatccttt gcggtcgatg tcgtcgacga ggggtttgag cttggttttg
     1501 acgagttcgg caacgttggc aagcagggtt tgggcgttca tctttgttcc ttaaggttta
     1561 cgggggaatc gggggcgcgc ctgatgcgcg gctgcttttc ccgccgcttg ggcggcttgt
     1621 ttttgtttga gggggtgttt ttgaaaaacc gcctgcattc aggcggtttt gtttgcttag
     1681 gcgtatgcct gcaatttggg gttgatttcg gtttgggcga ggttgttggc gtagttgcac
     1741 aaagttgcca aggctacgcc cattacgact tcgactgcct gctgccggtt gtagcccgca
     1801 tcgaaaaatg ctttgagttc gtcgtcggat actgcgcctt ttttcgccat tacggcttgg
     1861 gtaaaggcgg caagcgcgcc gagtttggca tcgtcaaatt cacctgccgc caaagcgcgg
     1921 gcggcattga gggattgctc ggacaggagt tttttcaggg ttgcgagttt ggtgtgccct
     1981 gccacgcaga agccgcattg gttggtgcgt gcggcaatga tccggatcac ttcgacttcg
     2041 ccggcggtca ggctgttggc ggcgttgagc ttgccgactt cttggtaaaa cgccaaggct
     2101 tcgggggcgt ttgccaatac gccgatgagg ttggggataa agccgttgtt tttgagtacg
     2161 gcctctacgc gcggtttggc ggcttcgggg gcggtttcga gggtgtgtac ggttaaacgt
     2221 gccattttct ttccttgttc gcaaatattg gggacgggcg catggtacgc atttcggaac
     2281 ggaataggaa agactgtgcc acaaacaaaa agttataaga aatgccgcct gaagcctttc
     2341 agacggcatt gccgggaaac acgcctaccc gcccccgcgt ccgaaatatt gcgccaatgc
     2401 ggcttggggg ttggcggctt cgactgtttc gggcaattcg gtatagccgc gcgataaaag
     2461 gtggcgtgca taaaggctgt tgccctttgc gccgaaccag acggaaaccg ccaagcccaa
     2521 tacgttaaac gccgagtctg tggcgggcgt gtcgccgtaa accagttggg caaacaccgc
     2581 caacacgaac atcgcgcccg tcatccccag ccccaaaccc cacattcttt tgaaacacgc
     2641 ccacagcgtg ccgaacaaaa gacccggcca cgaccagcct tgtttgacgg cttggggcgg
     2701 cagggcggga tgggtgtaga ttttgtatgg tttcatcgtg tttccttttc ggttgaaacc
     2761 ctgccctttg ggaaggtagg atcagacttt atttgtgaga cgcgcgcccg tgtgcagaca
     2821 tacggcttga acatgcccgt cggggaagac agatccgaca atgccgtctg aacggtgttt
     2881 tgccttcaga cggcattgcc tgccttcaaa gcggacgcgc ttattccgcc cagtttttct
     2941 ttttgctggt tttgccgata cccgggttga agctgttggt cgggtcgagt ttgcggtaaa
     3001 actgtttgag cgcgggcttg gcttcataca aatggccgac gttgtgttcg gcgggatatt
     3061 gcgcgccgcg ttggtctaag agatgcagca tttcgtgttc caacgccata cagccgttgc
     3121 cttttttgat gatgtaatcc tgatggaaaa cgtggcacat aaaatgcccg taatacaatt
     3181 tatggatgat tttattgtcg atttccggcg gcagtttttc aaaccagtcg cggtcgtcgc
     3241 ggcgcagggc gatatccagc gcgaccaagt cctccacttc gtcgtcgtgc acggaacggt
     3301 agcggatggc ggcggaggcg acggcgaaac ggtgcagcat ggcggcttgg gtttcttcgg
     3361 cgttgcattc gaaaaacgcg ccgccgtggt gtgaaaaata ttcttttaag aacgcgcgcg
     3421 cctcatccac gccttttccg cccattttca gaatcaggtg gtgttcgtat ttgtcgcggt
     3481 aatcgcgcat ggatttgggc aggtggtcgg gcaggaattt gctgacgaac tgcattgcct
     3541 tgtcggaaaa atgtttgggc aggaagctga cttttttgcc gaacctgtcc acgcgcgcct
     3601 tcaagtcaaa taatttcggc agttggtgcg taccgaattt tttgatgacg tagaacgtgt
     3661 ctttgccgta cacgtcggca atgtcgaaag cgtggcggtg gatgtattcg ccggaaacgg
     3721 gcaggctttc aaattcgccc aaggcggcgc ggcggatgtc ggtcagctcg ttgatgtcgt
     3781 tcgtgccgat gtagaacacg gcggtttgtt tttcttgcgg gaaggtgtcc aaacggacgg
     3841 caaaaaccat cagcttgccc gcgcagcccg aggcttcgta atggcgggcg gggtcggcat
     3901 tgaaacgcgc ggcggtcggt tcgtccactt ggcggacgtg ttcgcaatag gcgtggtcat
     3961 gtcctttgcc cgcgtcttgt ttgatgtctt ttttctgata atgatgacct tgcaggttgg
     4021 tcaggatttc ttcgggcatg tcgcccaagt ctatgcccaa gtggttgacc agttccaacc
     4081 tgccctcttc gttgatttgg gcgaacaacg ccatttcggt gtaggccggg ccgcgctgta
     4141 ccaacgcgcc gccggagttg ttgcacacgc cgcctaaaac ggacgcgccg atgcaagaag
     4201 agccgatgac cgaatgcggt tctcgtccca aaggtttcag cagcagttca agctggttca
     4261 gggtcgagcc gggcaggcag acgacttgtt cgttgttgtt gatggtttgg atgatgttca
     4321 tccgcatggt gttcacaatc acgatgtcgc ggtcgtaatc gttgccgtcg ggggtcgagc
     4381 cgccggtcag tccggtattc gccgcctgcg taatcacaat cacgtccgct tcgacgcagg
     4441 cctgcagaat tttccacatt tccaaaatgc ttcccgggcg caccaccgcc aacgccttgc
     4501 cttcgccgaa acggtagcct tggcggtatt gttcggtttt cgcggggtcg gtgatgatgt
     4561 atttttcgcc tacggtttgg gtcaggctgc tgattaattg cgaggcgttc ataacactct
     4621 cctatcggga taagcggcgg cgcaaatccc ggtacgccgc cgtatgttca atttggatat
     4681 tttggtgcgg caagttgcac gctgcaaatt atttttggtt aaagaaataa tgtaatttaa
     4741 tatttttggc atttgccgcg ttttccaaat cggccttgtc gctttcgcta tcgcccgtca
     4801 tatagacact gacattattt ccgactttct ttgcatggtt taaggaataa agtgcggtcg
     4861 ccttattaaa catttggctg cctcccgcca cgccgacgtt gatattgtcc gcgccgagca
     4921 tttcaatcat atttgatgcc gaaggcgatc cggatgtttt aatcattccg acagccaccg
     4981 catcggaaag cgggttattc gtcgcgggaa cctgcacgac aacagagcgt tctccctgcg
     5041 gcatttcaac catcaaccgg ccgacattga cacctgttga acaagctgca agcataccgg
     5101 tgagggttat cattagaagt tttttcattt gcctactcct ttagtcttta atcaaaccgc
     5161 cctgttcgtc atataacttg aaattgaacc atacttccat ttttatgaag ccggttcttc
     5221 attggcctgt atggatacat tgtaatgatg cgttatggca aagtaaagga aatgccgtct
     5281 gaacggcttt cagacggcat cggtgcgata cgggaacgcc ggaacatcga agctccggcg
     5341 tttcaaatag ggcggcggac caaacccccg gcactggcgc atcggagtgg gctgctggct
     5401 tccgcccctg acccggtgtt ccgatttgcc atgcggggag acccgcctcg gagaaacggc
     5461 attataacgg gttttctgaa aaactcaacc gttttgatac ggtcatacgc cggaaacacc
     5521 gcctaaaatt tatatttgat aatattgtca acaatttctc aaagcgttat tttgtttcta
     5581 taagggtatt gcctgtttcg gcattgaaaa atatcaaaaa ttgaactaca ttatcgcctt
     5641 ttcaaacccg gctgaaaccg acttttcaaa cggcattcaa ataaaaactg ccaaacacgg
     5701 acacactatg accacgacta ccgccccgca gcgtattcgg gaaatcccct acaactatac
     5761 ttcctacacc gaccgcgaaa tcgtcatccg cctgctgggc gacgaggcgt ggcacattct
     5821 acaggacttg cgcggacagc gcaaaaccgg gcgttcggca cggatgctgt ttgaagtgtt
     5881 gggcgatatt tgggtggtcg tacgcaatcc gtatctggtc gatgatttgc tggagcatcc
     5941 gaaacgccgc gccgcgctgg tgcgggaaat gcgccacagg ttgaacgaaa tccgcaaacg
     6001 ccgcgacgat aatcagcaag cggatgtgtt gattgcggcg gcggaaaagg gagtggagct
     6061 ttttgacggc agctttgacg aaacgcgcca aaagcggcgg cagattttgg agcgtttgag
     6121 caaaatcacc aagccgcaca atattatgtt tgacgggctg gcgcgggtaa cacacgttac
     6181 cgatgcgacc gactggcgcg tcgaatatcc gtttgtcgtc gtcaatcccg acacggaggc
     6241 ggaagtcgcg cctttggtgc gcgccttaat cgagctggac ttagttatta tcccgcgcgg
     6301 cggcggtacg ggttataccg gcggcgcggt acctttggac gcaaacagcg cagtcatcaa
     6361 taccgaaaaa ctcgacaagc atcgcagcgt cgaatacgtt gagctggcag gcttgtacgg
     6421 cagacatccg attatccggt gcggcgcggg cgtggttacg cggcgggtgg aagaaaccgc
     6481 gcatcaggcg ggcttggtgt tcgccgtcga tccgacttcc gccgacgcgt cctgcgtggg
     6541 tggtaatgtg gcgatgaatg cgggcggtaa aaaagccgta ttgtggggta ctgccttgga
     6601 caacctcgcc tactggaaca tggttaaccc tcaaggcgaa tggctgcgta tcgaacgcgt
     6661 gcgccacaat ttcggcaaaa tccacgacga agaaactgcc gtgttcgacg tgcacacgct
     6721 ggattcagac ggcatcaaca tcgttaaaac cgaacgcttg gaaatccccg gacacaaatt
     6781 ccgcaaagtc ggtttgggca aagacgttac cgacaaattt ttgagcggac tgcccggcgt
     6841 gcagaaagaa ggtacggacg gcatcattac cggcgttgcc ttcgtattgc acaaaatgcc
     6901 gaaatacacg cgcaccgtgt gtatggagtt tttcggcacg gccgccaccg ccacgccttc
     6961 tatcgtcgaa atccgcgatt ttctgctcgc acacgacagc gtgcggctgg cgggtttgga
     7021 gcatttggac tggcgttatg tccgcgccgt cggctacgcc accaaagcgg cgggcaaagg
     7081 acgaccgaaa atggtcttgc tggcggacgt ggtttcagac gacgaagccg ccgtagaggc
     7141 agccgccgaa cacatctgcg aacttgcccg cgcccgtgac ggcgaaggct ttatcgccgt
     7201 atcgcccgaa gcgcgcaaaa ccttttggct cgaccgcagc cgcaccgccg ccatcgccaa
     7261 acacaccaac gcctttaaaa tcaacgaaga cgtggtgatt ccgctggaga ggctgggcga
     7321 gtattcggac ggcatcgaac gcatcaacat cgagctttcc atccaaaaca aactcaaact
     7381 ctgtgccgct ttggagcaat atctttcggg caaactcccc atcgacaaaa tgggcaccga
     7441 cctgccgacc gccgaactgt tgggcgaacg cggcaaacac gccctcgccc acgttgccgc
     7501 cgtcaaagcg cgttgggact ggctgctcgc ccatttggat gcgccgcttg ccgactacaa
     7561 atcacgctac ggtacagccg tccacgccgc gcccgaagcc aaagacgatg aaagctgctt
     7621 caccgccttc cgcgatttcc gcctgcgcgt gtctgtcaaa gcagacgtaa tgaagccgct
     7681 tgccgaaatc ttcagcggca aaaccgatac caaaattatc caaggcttgg gcaaaatcca
     7741 cgccaaaacc gtacgcagcc gcgtctttgt cgccctgcat atgcatgccg gcgacggtaa
     7801 cgtccacacc aatattccgg tcaactcaga cgatgccgaa atgcttcaga cggcctaccg
     7861 ttctgtggaa cgcattatga aaatcgcccg ttcgctcggc ggcgtgattt ccggcgaaca
     7921 cggcatcggc atcaccaagc tcgaattcct caccgatgaa gatttgcaac cgttttggaa
     7981 ctacaaaaac caagtcgacc ccaagcacac cttcaaccgc cacaaactga tgaaaggttc
     8041 ggacttacgc aacgcctaca cgccgtcttt cgagctgttg ggcgcggaat cgctgattat
     8101 ggaaaaatca gacctcggca cgattgccga ttccgtcaaa gactgcctgc gctgcggcaa
     8161 atgtaaaccc gtctgctcca cccacgttcc gcgcgccaac ctgctgtaca gcccgcgcaa
     8221 caaaatcctc ggcgtgggtt tgctgaccga ggccttctta tacgaagaac aaacccggcg
     8281 cggcgtttcc gtcaaacatt ttgaagaact gatggacatc ggcgaccact gcaccgtgtg
     8341 ccaccgctgc gtcaaaccct gccccgtcaa catcgacttc ggcgacgtta ccgtagccgt
     8401 ccgcaactac cttgccgatt ccggccacaa acgctttgcg cctgcggctt caatgggtat
     8461 ggcgtttttg aacgccaccg gtccgaaaac catcaaagcc ctgcgcgccg cgatgataca
     8521 gaccggcttc cccgcgcaga actttgccta caaaatcggc aagcttcttc cgatcggcac
     8581 gaaaaagcaa aaagccgaac ccaaggcaac cgtcggcaaa gccccgatta aagaacaggt
     8641 tatccatttc atcaaccgcc cactgcccaa aagcgtaccc gccaaaacac cgcgctcctt
     8701 attgggcatc gaagacggca aaagcatccc catcatccac aaccccgccg cgcccgaaga
     8761 tgccgaagcc gtgttctact tccccggctg cggttcggag cgtttgttca gccaaatcgg
     8821 actcgccgtc caagccatgc tctggcacgt cggcgtacaa accgtcctgc cgcccggcta
     8881 tatgtgttgc ggctatccgc aagacgctgg cggcaacaag gcaaaagccg aagaaatgag
     8941 caccaacaac cgcgtcgcct tccaccgtat ggcgaacacc ctcaactacc tcgacatcaa
     9001 aaccgtcgtc gtcagttgcg gcacttgtta cgaccagctt gaaaaatacc gcttcgagga
     9061 aatcttcccc ggctgccgca tcatcgacat ccacgaatac ctgctcgaaa aaggcgtgaa
     9121 actcgacggc gtgaaaggtc agcaatacct ctaccacgac ccctgccaca ccccgattaa
     9181 aaccatgaac gccacccaaa tggccagcag cctgatgggg cagaaagtcg ttttaagcga
     9241 ccgctgctgc ggcgaatccg gtatgttcgc cgtcaaacgc cccgacatcg ccacacaggt
     9301 caagttccgc aaacaagagg aaatcgagaa aaacctcaaa gagctgccgc agggcgaacc
     9361 cgtcaaaatg ctgacctcct gccccgcctg cctgcaagga ctgagccgct attccgacga
     9421 caacaatatg cctgccgact acatcgtcat tgaaatggcg aaacacatcc tcggcgaaaa
     9481 ctggctggat gagtttgtaa aaaaagccaa caacggcggc gtagagaaag tgttgctata
     9541 acaacggaca cggaaatgcc gtctgaacgc cgaaagcctt cagacggcat tgtttgaatc
     9601 aaatacctac ccgaatcatg attgacggac aaacaaccga acccaaacaa aaaaccagaa
     9661 tcataccagc ccccatgcgg gggctggtag acgatgtgat gcgcgacctg ctgacacgta
     9721 tcggcggcta cgacgaatgc gtcagcgaat ttgtacgcat tacccatacc gtgcattcac
     9781 gatccatatg gttaaaatat gtccccgaaa tcgccaacgg aaacaaaacg ttttccggca
     9841 cgccctgcac cgtccaactt ctgggcagcg atgcggacaa tatggcggca aacgcattgg
     9901 aagccgtccg attcggcgcg gacaaaatcg atttgaactt cggctgcccc gcgcccaccg
     9961 tcaacaaaca caaaggcggc gcaattcttc tgaaagagcc ggaactgata ttccacatcg
    10021 tcaaaacgct gcgcggacgt ttgcctgcac atattccgct gacggggaaa atgcggctag
    10081 gctatgaaga caaaagccct gctttggaat gcgcctgtgc gattgccgaa gggggcgcgt
    10141 gcggactgac cgtgcacgcg cgcaccaaag ccgagggcta cgaacccccg gcgcattggg
    10201 aatggataag gaaaatccac gataccgtca atattcccgt taccgccaac ggcgacgttt
    10261 tcagcctgca ggactatatc ggcatcaaaa caatcagcgg ctgcaacagc gtgatgctcg
    10321 gtcgcggtgc ggtcatccgc cccgatttgg cgcgccaaat caagcaatac gagaacggcg
    10381 gaccggtcaa agacacggat tttgccgaag tttccacatg gatagggcag tttttcgagc
    10441 tgtgcctgac aaaagaggca aacaacaaat acccgatcgc ccgtcttaag caatggctgg
    10501 gcatgatgaa aaaaacgttc gacccggcgc aaacgctgtt cgaccgcgtc cgaacggtta
    10561 aagatgcgga tgaagtgcgc cgaatcctga acgcatttga gcatgagatg gatgtttgat
    10621 gtctcccgcg cttcaaagca aatgccgtct gaaaaccttt cagacggcat ttgttatctt
    10681 tattgccgtt tttcccctgc tttcggtgta tttttgtttt tcagtttcgc ccccaaaccc
    10741 aaacgttttt cataatccga ttgcggggta tcgtcttcct gcataccgaa cgcgccggca
    10801 ttgacccaca gcgacagcag cgcgacgaca aaggcgcaaa agccgattac ataccaaaac
    10861 attgcccctc caaatttgtt aaaatcatat caaatacagt gccgaattta tcacaagcgc
    10921 acgagcaaat aaatcttctg accgcagtat ttcattcatt taccttgaaa acaaaaaagg
    10981 gcacctgaaa attttgcgaa acttttcaga tgccctgctt tgccgaaaaa cgtatcagcc
    11041 gcagcaaccg ccttcgccgt gtccgccttt tttgtcgtta tcgacaatgg gcagttcgac
    11101 tttttcgttt ggtttttctg cattttcggg ccggtcgttg agtttgattt tcgcggtttc
    11161 gctcatttcc ttacctcatt tggaaaacgg tttgaaagat tcataaatat agcatgttta
    11221 atccgaatct gaaaagggaa gccgtatgcg aaatcccgtc tgaagcccaa tcagggcttc
    11281 agacgggatt tgctatcttt attgccgctt ttcttccgta tccggatttt tgtttggggc
    11341 tgaagcagat tggcagtcag attgcaatcg aagaatgaag gcaagccatc aaaaacaaag
    11401 ctatccgctt caccgccccg atatttagaa tttgtggcgc agaccaacgg aggcggcatt
    11461 aatttgagtg tagttgccga tgccggtatt tcgtttcagc caagcggcag acatgatggc
    11521 ggaagtgcgc ttggaaaaat cgtaatcgac accggcgatg atttgatcat agctggtatg
    11581 ttcgcgtttc tgactgcgtt cgacaaagtc gaaaccatgg gcatagctga tgcgcgggac
    11641 tgtattaccg aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt
    11701 gtcggcattt tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcccc
    11761 atagccgccc gtcaggcggt gtacctgatg gtttttcaat ggatcggtat cactcgctct
    11821 gccgagcaag aacaaaaaga aagcatcatg cccctcattg gcgtgtttcg catatttaag
    11881 ggcataattt ccgaaaaagc cgccattttt gtaattcaga ccggcataat acacatccga
    11941 ccccggcttg ccgacaacag ccggaactgg tttcatctga ttactttcca gcgtgaaagt
    12001 agcaggcgta taggcggact tgctgttttg actcggaaca aattgaatgc tgccgctgaa
    12061 accggaaaat ccgggggaat cgtaacgcac ggaaaccggc ataccgtcgt ggcgtttgaa
    12121 aatacccaat tgcgaagcca cattattatt gctgtcccaa ggatcaatgg ctttgctggc
    12181 atcgccaaac ggattcgcaa cgcgaccggc gagcgccgtg ccgaattcgc ctgccaagcc
    12241 gataaaggat tccctgttac cccaacgggt cgcgccgccg ccggcaacgg atacgtcttg
    12301 ctcgagctgc caaacagcct tcagcccgcc gcccaaatcc ccaccccctt aaagccgata
    12361 aacgagccga aatcactgac tttcgtcctg atgcggcttt tggccttagt aactgtattg
    12421 cccgtttgac cttctgaggg tggctcggtc aactgcagcc ggatgttcct gccttccaca
    12481 ccagctttga cttcgccgta caggctgaca tcggcaactg ccgcaaacgg cagtgcggac
    12541 aatacgaggg cggtaagttt ttttcgcata tcggcttcct tttgtaaatt tgctaaaaac
    12601 atcgggcaaa cacccaatca gtctccaatt accccccgaa aaaaaaacca tttttcggaa
    12661 caaatttcgg gtaaattttg gaatttttat tttgaaaaat aatatttcaa tataaaacaa
    12721 taaattaact tttttgaaaa tgttgctttc ccgcaacatg atttaaatac agaccttgtc
    12781 aaagccgatg cggcgcagac ccggatgccg tctcgttttt taaaacacca taaaaaaaca
    12841 cacgcggtgc tttaatttcc tgcgtgtgtc gagtgtatcg aaatcaagcc gaatcaaata
    12901 taccggactt cgataatgcc gtattcgcgt acgccgcccg gtgcctgaac ttccgccgta
    12961 tccccttctt ccttgccgat cagggcgcgg gcaatcggag agccgacata gattttgccc
    13021 tgtttgatgt cggcttcgtc ttcgccgaca atttgatagg taacgtgttc ttccgtttcc
    13081 aaatcttcca gcgtaaccgt cgtaccgaac acgattttgc cttcggcgtg gatttcggcc
    13141 ggattgatga tgtgggcaac ggaaagtttg tgttccaact cggaaatgcg gccctcgata
    13201 aaaccttggc gttctttggc ggcttcgtat tcggcgtttt cggacaaatc gccgtgcgat
    13261 ctggcttcgg caatcgcttc gatcacttcg ggacgcgcca cgcttttgag ctgctgcaat
    13321 tcctgtttca gcaattccgc accgcgtacg gtcaggggga ttttttgcat cggtcttttt
    13381 ctccatattc cggcacaccg gtttgcggcg gcaagcatac cgcgtaccgt cttgttttgt
    13441 gcgtccggat attaaaataa aaatacaagc cgcccggaaa atcggcggct tgtctgtcgt
    13501 tgaacagcgg ctattctacc aaattctatg aaattggcaa tcgtgccgtg ccgccggcaa
    13561 acgcgctatg tccgcaacaa aagccgaaaa tatgccggca aaaaaatttt agaaacaaaa
    13621 aatttaaaaa aaaatcaatt ttcggcataa aaaaccaaat tcacgtactt taaaaccgaa
    13681 aatgccaaac ctgagatttt ccagacggca tttgcaccag tataatgcag gctgttttta
    13741 tctttatatt tacgttttgc catgaccgaa tccgtccgcc tccccgccgc ctcgcccaaa
    13801 ccttccaccg tcgccctgcc cggctccaaa agcatcagca accgcaccct gctgctcgcc
    13861 gccctgtccg acaatgtttg cgaaatccat tccctgctca aatccgacga taccgaccgt
    13921 atgctcgaag cactcgataa actcggcgtt caaatcgaac atcttgccga aggccgtctg
    13981 aaagtacacg gcacgggcgg acgcttcccc aaccgctctg ccgatttgtt tttgggcaac
    14041 gcgggcacgg cgttccgtcc gctgaccgcc gcgctggccg ttttgggcgg cgattatcat
    14101 ctgcacggcg tgcctcgtat gcacgaacgc cccatcggcg atttggtcga tgcgttgcgg
    14161 attgccggcg ccgatgtcga atatctcggc aacgaacact atccgccgct tcatgtcggc
    14221 gaacgtgaag actgcggcga gcgcgtgatt ccgattaaag gcaatgtgtc cagccagttt
    14281 ctgaccgccc ttttgatggc gttgccgctg accgggcagg cgtttgaaat ccgtatggtc
    14341 ggcgagttga tttccaagcc ctatatcgac attactttga aactgatggc gcaattcggc
    14401 gtacaggttg ccaatgaagg ctaccgcgtt ttcaaaatcc ctgccgatgc gcactatcac
    14461 gcacccgaac acttgcacgt cgaaggcgat gcctccggcg cgtcctactt cctcgcagcc
    14521 ggtttgattg ccgccacgcc cgtccgcgtt accggcatcg gcgcaaacag catacagggc
    14581 gatgtcgcct ttgcccgcga actggaaaaa atcggtgcgg acgtggtttg gggcgaaaac
    14641 ttcgtcgaag tctcgcgccc gaaaggacgc gccgtccaag cctttgattt ggatgcgaac
    14701 catatccccg atgccgcgat gaccctcgcc atcgtcgcgc ttgccacaag gcaaacctgc
    14761 acgcttcgca acatcggttc gtggcgcgtt aaggaaaccg accgcatcgc cgcaatggcg
    14821 aacgagttgc gcaaactcgg cgcaaaagtc gtcgaagaag ccgaagcgat tcacattacc
    14881 ccgcccgaaa cgccgacacc cgatgccgtc atcgacactt acgacgacca ccgcatggcg
    14941 atgtgtttct cgctgatttc gctgttggga gtacccgtcg tcatcaacga cccgaaatgc
    15001 acccataaaa ccttcccgac ttattttgaa gtgttctcat cactgaccga aacagcggaa
    15061 taaggcggca aaatgccgtc tgaaacccga aaacagggct tcagacggca ttttgccgcg
    15121 attccggcgc ggcgggcggc tcattctgta aaaaacgtat gcgctccaag gtagtttttg
    15181 gcgtaaaacg gtgtggagag tttttcggtt ttgatggttt tgccgctgcc gggggcatgg
    15241 atgaattcgc cgttgccgat gtagagtccg acgtgtgagt agcggtgtgc gccgccggtg
    15301 ttgaagaata cgaggtcgcc ggccttgagg cggctgtcgg ggattttgcg gcttgccgcc
    15361 gccatgtcgc gggcggtgcg cggcagcttg acgttgaggg cgtttttgta aacgaattga
    15421 atcatgccgc tgcagtcgaa gccggttgcg gtgctgctgc cgccccattt gtagggcgtg
    15481 ccgatgagtc cgaggctgtg gagcatgagt tcctgcgagc cttgtgtgcg gccgatgtgg
    15541 ctgatgcgga cggcttggat ttgccggact gtctgtttgg gtttcggttg gcggtgtttg
    15601 ccggaggtcg tgccgcatga ggcgaggagc agtgcgccga gacagaggaa aagggttttg
    15661 tcgggggaaa acatggtttt ttcctttgcg ggttcggata tgccgtctga aggtgtttca
    15721 gacggcattt ctataataaa ccttcgatgg gcagcaggga taggattttt gcggcgatgc
    15781 gtttccaaag tttggcttcg ggttcgttcg ggtaggtttt tcgggtggcg ggatcgtgcc
    15841 attgcaggcg gttgtgtttg tcgagggtaa cgcggtaggc gtattcgggt gtggtatcgg
    15901 cgagggtgcg ctccatctgt tctgcgattt tggggctttc gatgacgacg cccatttcgg
    15961 tattgagccg tgcggaacgg gggtcgaggt tgaatgagcc gatgaagatg cgtttgccgt
    16021 ccacaatgaa ggttttggca tgcaggctgg ttacggagct gccggtcagg cctttgtctt
    16081 ttgtggcggg gacggcatgg ttgggttgca gctcgtagag tttgatgccg gctttgagca
    16141 gcggttttcg gtatttgacg tagccggaat ggacggcggc aacgtcggtc gcctgtagcg
    16201 agttggtcag gacggtaacg tctatgccgt cctgcaccag ttttgccagt gcgtctgtgc
    16261 cggattttgt agggacgaaa tagggtgaaa ccagatagac gcttttttcg ggctgtttga
    16321 gcgcgtcttg cagcctcccg gcaatcggcg gtttgcggcg gtcgcggtcg agtccttttg
    16381 cagggttgtc gctgatcagg cgggtttgga cgctctgcca gtcgatgcgt cccgtctgta
    16441 ttttttggta gaggggcgac tgttcgacgg tttcgcggta gcgcaggagc gcgtgtctgg
    16501 atgtttcgtc gttgtatccg agtgcttgaa gacccttgcc gatgttgccg ctgcggatga
    16561 tgcgcgtggc gttgtgggcg gaatggcttg cccagtagcg gtcgaagtcg tgcgatactt
    16621 cgccgacgac gctgccggtg gcgaggatgt ccaggtcggc gaaaacggtg tcctcaccga
    16681 ctttgaagta ttcgtcgccg atattgcgtc cgccgagtat ggtggcgcgg ttgtcggcgg
    16741 taaaggattt gttgtgcatg cggcggttga ggcgggggaa gtcggtcagg tagccgagtg
    16801 cgcgccattt gcgtaggacg aaggggttga acaggcgcac ttcgatattg ggatggctgt
    16861 cgagggcgag caggagatcg tccaacccgc gcgtgttgtt gtcgtccaac agcaggcgta
    16921 cgcgcacgcc gcgttctgcg gcaaggtaca tgaggttgaa cagcagcctg ccggaaatgt
    16981 cgttgcgcca aatgtagtat tgcaaatcga ggctgtgttc ggcagattcg ataagggcgg
    17041 cgcgggcggc aaaggcttcg tgggggtcgt cgagcaggta gatgtcggat agcccgttgt
    17101 tatgaggggt gtgccggatt tgcaggatgt tgtccaggag gacaggtttg gaagtattga
    17161 aatgacggct ttccgtccgt tcttccagtg ggggcaacca tgaagaacat gaacagagaa
    17221 ggaggcataa aagggaaatg aggctgcgtg ttttcatcgg ggatatagtt tcagacggca
    17281 ttgcctgtat tttggggtct gtatgcattg aaatgaagca tcataatcta aaggttggaa
    17341 tgggtaaaag ttttgtcggg atgcggggtt aaggccgcgt gcctcttgtc aggtttgcac
    17401 cgtctgaaac gtgcagacgc ttaaaaacca acgacgaggc aagggtgatg ccgccgatac
    17461 tgagcagggt catacggaag gcgaaatgca ggcctgaaga agccggtatc agaaatgtcc
    17521 agtttttaag gattaatgcg ccggcaacaa tgcccacgct gatggcaagc tgttggttga
    17581 ccgccatcag gctgttgccg ctgccggtct gttgcgggcg caaatcggcg agggtcagtg
    17641 tgttcatggc agaaaactgt agggagttgc atgcgccgat cgccagcgag aggaaaatcc
    17701 aaatccacag cggcgagttt ccgtcaggca gggcgagcag catgatgaag gcggcaagca
    17761 gcttggtgtt ccaaagcagt accgtgcggt agccgaaacg tttcatgagc ggtgcaatca
    17821 gcggtttgac cagcagcgaa gacagggcga cgggtgcgac cagccagccc gacaggcttg
    17881 cgccgaagcc gaaagcgact tgaaacatca ggggcatcag aaaaggaatc gagctgatgc
    17941 cgagacggct gaacagattg cccgccagtc ccaaacggaa ggtgcgtatc agaaacaggt
    18001 cggcggaata aatcggtttg gacgcggttt tcatatgtcg gaaataacgg cgtgcaaaca
    18061 gcagtccgcc gcacagcggt aacagtgcaa aatacggagg cagcgcgtgc gacaggcttt
    18121 ctgccgcaag cagcagcagg caggcggcaa cggaaaaggt cagatagcct ttgaaatcca
    18181 gagcggtatc gttgcctttg acatcgggca tgatgttgcg tcccaatacg aaacccagca
    18241 gaccgatggg caggttgagc aggaaaatcc agtgccacga agcgtattcg accagataac
    18301 cgcccgccag aggtcccaac gcagggccga tcagtgcagg catgaccgca taattgatgg
    18361 cgttgagcag cttcgatttt tcatatacac gcaagatggt cagacgcggt atcggaacca
    18421 gcatcgaacc gccgatcccc tgaacgacac gggaaagcgt caattcaaac agcgaacccg
    18481 atgcggcgca caatgccgat ccgagcataa aaacggcaat cgaaccgaaa aagacttttt
    18541 tcgttccgaa cttgtccgcc aaataactgc tcaaaggaat cagcagggca accgtcagcg
    18601 tataggcaac gactgcctgt tgcatatccg gaggcgattc gttcaggtcg gcggcaattt
    18661 cgggcagtgc ggtatttaaa atggtcgcat ccaacatctg cataaaaatg gcaattgcca
    18721 gcagaagcgg cagccaaggg gatggtgcgc gggcggacag ggtgtttttt tccatagggt
    18781 gattgtaccc catccttgtg ccgttattgt tttcagatgc tgtctgaatg ccggcagagt
    18841 cggcatcctg aatgttcaca agcaaacgaa tcggcattgc attgtaatga taattattat
    18901 cgaaaatcat cagagtaagg tacagtaagc gttatggggc agtttgtaag aaaaaccgga
    18961 ttatttttta aaattagact tgacccgcaa cggtcaatta cttaaagtaa acgcttacct
    19021 ttctacagaa aaaacgggtc tcccgttatc aaaaaatatg agcgcaacca ttcccccaaa
    19081 aatcatccga tacgacagca atccgacaga tgtctatttt ttcggcactt gcgtccttga
    19141 tctttttatg cccgaagcag gcatggatgc cattgcccta atcgagcagc agggcatacg
    19201 cgtccatttc ccgatggcgc aaagctgctg cggtcagcct gcctattcat ccggccatcc
    19261 gaccgaagcc ttcgatgtcg ccaaagcgca actcgacctt ttccccgaaa actggccgat
    19321 tgtcgtaccg tccggctcat gcggcggcat gatgaaacat cactggccta ccctgtttaa
    19381 aaatacagaa tacgaatcca aagcggttga ttgtgccaac cgtatcattg agtttactca
    19441 tttcttgctt gccatcggct acaagcctga agacaaaggc gagccagtca aagtcgccgt
    19501 tcacacttcc tgtgccgccc gccgagaaat gaatgtccat ctttcaggct ggcagttgat
    19561 tgacggtatg gaaaacgtcg aacgcatcgt ccacgaccac gaaagcgagt gttgcggctt
    19621 cggcggcaca ttctccgtca aacaagccga tatttccggc gcgatggtaa ccgacaaagt
    19681 tgccgcgctg aaagaaaccg gcgcaaccga aatcatcagc gcggactgcg gctgtatgat
    19741 gaacatcgga ggcaaaatcg ccaaggacga gccggatatg ccgcgtccga agcatatcgc
    19801 atccttcttg ttggaacgca ccggaggcaa agtatgagcg cgcgcgaaaa tattttggca
    19861 aaactgaaaa aagccggcgc attgccgatg gaagagcctg cggtttttga ttattaccgc
    19921 gaaaagggcg tttcttggga cagcgaagcc gagcgtctga aacattgggc tgccgccatg
    19981 cgcgcggtca aaaccgaaat ctattgggtg acgaaaagca attggatgca ggttttccgc
    20041 gaagcggcag aaggcaaggg tttaaaaaac atcctgctgc ccttggcaac cgaacacgga
    20101 caaattgccc gtgccgcatt ggcgggcagc aatatcgacc cgattgcctt cgagcgcgaa
    20161 atcgatactt ggaaaaccga gtttttcacg aacatcgatg caggcttcag cggcgcgcaa
    20221 tgcggcatcg cccgcaccgg cacgctgatg ttgttttcca gccctgaaga accgcgtact
    20281 ttgagcctcg ttccgcccgt gcatttctgc ctgttcgata cgtccaagat gtacaacgag
    20341 tttcacaacg ccgtcgaagg cgaaaaactg gtggaaaacg gtatgccgac caatgtattc
    20401 ctgatttccg gcccgtccaa aaccgcagac atccagttga cgctcgctta cggtgcgcac
    20461 ggtccgcgtg atttggtcat cctcgccatt ttgcccgacc acatttcccc tgccgatttg
    20521 gaggaaaacg catgactacg caaaccatca agttccacat gaagccggaa actttcaaac
    20581 aaaacgccgc aatttccctt caagacaagc ccttgcgtaa aagcctgcgt accgcgatgg
    20641 atatgctgat gaccaagcgc aaagccgttt tgaccgacga agaagagttg caaagcctgc
    20701 gcgatttgtg cgaacacatc cgtcagcgtt ccctctccaa attacccgcg ctgctggagc
    20761 agttggaaga aaacctgact aagttgggcg tgaaagtaca ctgggcggaa acccctgccg
    20821 aagcctgcca aattatccac gacatcatca cagccaaaaa cggcaagctg atggtcaaag
    20881 gcaaatccat ggtcagcgaa gaaatcgagc tgaaccatta tcttgaagca aaaggcatta
    20941 aggcggtgga aagcgacttg ggcgagttta tcgtccaaat ggcaggcgaa aaaccgaccc
    21001 atatcgtgat gcccgcaatc cataaaacca aagaacaggt cagcgaactg ttccaccaaa
    21061 acctcggcac accgcttaca gacgacgtgg accaactgac cggattcgcc cgcaaagccc
    21121 tgcgcgatat ttacagcact gccgatgtcg gcttgagcgg cgtaaacttc gccgttgccg
    21181 aaacgggcac gctgtgtctg gtcgaaaacg aaggcaacgg ccgcttgagt actaccgttc
    21241 cgcccgtaca tatcgtcatt acgggtattg aaaaagttgt cgccaagctg tctgatgttc
    21301 cgcctttgta cagtctgctg ccgcgttccg ccatcggtca gaacattacc acttatttca
    21361 atatgattac cggcccgcgc cgcagtgaag aattagacgg tccgcaggaa atgcacttgg
    21421 ttttgctcga caacggccgc agccaagctt atgccgaaga ccaaatgcgc cgcaccctgc
    21481 aatgtatccg ttgcggcgcg tgtatgaacc attgcccggt ttatacacgc atcggcggcg
    21541 cggcatacgg cacaacctat cccggtccga ttggcgagat tatttccccg cacctgttgg
    21601 gtcttgatgc cacccgcgac ctgccgaccg cctgcacgat gtgcggcgcg tgcgtggaag
    21661 tttgtccggt acgcatcccg attaccgaac aaatgcaacg tttgcgcgtt gaagcacaac
    21721 gttcgccgac cgaaaccgtg ccgcacccca tccgagggca aggcgcatcg cataccttcg
    21781 gcgaacaaat ggcgtggcgc acattcaacg gtattttcag cggtagcaaa acctaccgcg
    21841 ccttcggttg ggcagccacc aagttccgca atctgacccc gcgcaaacag ttgggttgga
    21901 cgcaaaaccg cgtaccgatg aaaccggcga agaaaaccct gcacgaactg atggcggaaa
    21961 aaatgcgcca aaaagaacag gcataaaaag ttgttcgcaa aaataccgtc tgaaacccga
    22021 aaacagggct tcagacggcc tgaaaagcag cctgaacgcg caaaaataaa taatgaaacg
    22081 gaatgctaag acgctttcag gcagcctgac attaaggctg cttaaaagcg tcttttaatc
    22141 gtgccacgga ttgaacaccg cgacatcggc cgcaaaaaat gagccggtat cacgcgtagc
    22201 aactgtcaaa ctgtgctgtt ttgcagtggc ggcaatatag ccgtctgcgg cagcaatctc
    22261 tttgccatgt gttttggcat aggaacgaat ttgcgcatag attgcggcaa ccggttcatc
    22321 aaaaggcaga atccgccccg caaataaagg caaaatggat tgttccaaac gttcgtgcag
    22381 cacattcttt ttcttgccat tgagcaacaa cgccacaccc aaacgcaatt ctgcaacagt
    22441 aatggcagac aaatacacat cttccaatat caaactatcc aaccatgcca ccacacgttc
    22501 attgggttgt gggcgcaaag gttcggaaat cacattcgtg tccagcaaaa tcatcacaaa
    22561 gaaacctcgt tatcagtatt acgaccgcgt acgtcttcca gctcaacacc tccgatttcc
    22621 tgccctattg atgccaacat tgaccccaaa cgtacagttt gttgtgcttt ggcgatgtta
    22681 tccaaaatta agcggatttc tgcttcggta ctgcgccctg cggctcgcgc acggaatttg
    22741 attgcgttgt gcgtggcctc ggataaattt ctaatcacaa cagaagccat tgcctttcct
    22801 tcctgaaaaa agtttttcaa atgctatcaa aataaaaaaa atgatagcaa tctatttttt
    22861 aacgcctgcg gatgcctgaa aattcaggtg gccgcaggcg tttttttaca tctaaaaaaa
    22921 cgtctcccct ccctctttct gataaataaa aaacagtcgc tc
//
LOCUS       DAXFWM010000030        22683 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000030 DAXFWM010000000
VERSION     DAXFWM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22683
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.030"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(33..1250)
                     /locus_tag="ACFI8B_001098"
     CDS             complement(33..1250)
                     /locus_tag="ACFI8B_001098"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC3834334.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1452..2180)
                     /gene="rph"
                     /locus_tag="ACFI8B_001099"
     CDS             complement(1452..2180)
                     /gene="rph"
                     /locus_tag="ACFI8B_001099"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC3834335.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2262..2726)
                     /locus_tag="ACFI8B_001100"
     CDS             complement(2262..2726)
                     /locus_tag="ACFI8B_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC3834336.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            2944..3720
                     /locus_tag="ACFI8B_001101"
     CDS             2944..3720
                     /locus_tag="ACFI8B_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC3834337.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3724..3855
                     /locus_tag="ACFI8B_001102"
     CDS             3724..3855
                     /locus_tag="ACFI8B_001102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834338.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQSTPFQNICI"
     gene            3965..4819
                     /locus_tag="ACFI8B_001103"
     CDS             3965..4819
                     /locus_tag="ACFI8B_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC3834339.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            4860..6068
                     /gene="pncB"
                     /locus_tag="ACFI8B_001104"
     CDS             4860..6068
                     /gene="pncB"
                     /locus_tag="ACFI8B_001104"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC3834340.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6160..7878
                     /gene="argS"
                     /locus_tag="ACFI8B_001105"
     CDS             6160..7878
                     /gene="argS"
                     /locus_tag="ACFI8B_001105"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC3834341.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            8406..9824
                     /locus_tag="ACFI8B_001106"
     CDS             8406..9824
                     /locus_tag="ACFI8B_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC3834342.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLVPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            complement(10277..11023)
                     /locus_tag="ACFI8B_001107"
     CDS             complement(10277..11023)
                     /locus_tag="ACFI8B_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3834343.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11007..11186)
                     /locus_tag="ACFI8B_001108"
     CDS             complement(11007..11186)
                     /locus_tag="ACFI8B_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834344.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(11202..11873)
                     /locus_tag="ACFI8B_001109"
     CDS             complement(11202..11873)
                     /locus_tag="ACFI8B_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC3834345.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(11942..12613)
                     /gene="rpiA"
                     /locus_tag="ACFI8B_001110"
     CDS             complement(11942..12613)
                     /gene="rpiA"
                     /locus_tag="ACFI8B_001110"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC3834346.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12690..13172)
                     /gene="ispF"
                     /locus_tag="ACFI8B_001111"
     CDS             complement(12690..13172)
                     /gene="ispF"
                     /locus_tag="ACFI8B_001111"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC3834347.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDMTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13204..13893)
                     /gene="ispD"
                     /locus_tag="ACFI8B_001112"
     CDS             complement(13204..13893)
                     /gene="ispD"
                     /locus_tag="ACFI8B_001112"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC3834348.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTIKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLGGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(13890..14624)
                     /gene="dnaQ"
                     /locus_tag="ACFI8B_001113"
     CDS             complement(13890..14624)
                     /gene="dnaQ"
                     /locus_tag="ACFI8B_001113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC3834349.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(14780..16090)
                     /locus_tag="ACFI8B_001114"
     CDS             complement(14780..16090)
                     /locus_tag="ACFI8B_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3834350.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIRLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16080..16265)
                     /gene="ccoS"
                     /locus_tag="ACFI8B_001115"
     CDS             complement(16080..16265)
                     /gene="ccoS"
                     /locus_tag="ACFI8B_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC3834351.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16265..16564)
                     /locus_tag="ACFI8B_001116"
     CDS             complement(16265..16564)
                     /locus_tag="ACFI8B_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834352.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            16915..18111
                     /locus_tag="ACFI8B_001117"
     CDS             16915..18111
                     /locus_tag="ACFI8B_001117"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC3834353.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(18160..18282)
                     /locus_tag="ACFI8B_001118"
                     /pseudo
     CDS             complement(18160..18282)
                     /locus_tag="ACFI8B_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18624..20465)
                     /locus_tag="ACFI8B_001119"
     CDS             complement(18624..20465)
                     /locus_tag="ACFI8B_001119"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC3834354.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
     gene            20556..21026
                     /locus_tag="ACFI8B_001120"
     CDS             20556..21026
                     /locus_tag="ACFI8B_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC3834355.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(21099..21911)
                     /gene="hpnC"
                     /locus_tag="ACFI8B_001121"
     CDS             complement(21099..21911)
                     /gene="hpnC"
                     /locus_tag="ACFI8B_001121"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC3834356.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(21998..22480)
                     /locus_tag="ACFI8B_001122"
     CDS             complement(21998..22480)
                     /locus_tag="ACFI8B_001122"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3834357.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
BASE COUNT         5387 a         6740 c         5564 g         4992 t
ORIGIN      
        1 cagtgccgtt gtccgtttta aaccgtgaaa catcagccca aatcaaaggc tttatgcaat
       61 accctggttg ccagttccat gtatttttcg tcaatcaata cggaaacttt gatttcggag
      121 gtggaaatca tttggatgtt gatgccctct tcggcgagcg tgcggaagat tttggcggct
      181 acgccgacgt gcgaacgcat acccaaaccg actgcggaga ctttgcacac ggtgtcgtcg
      241 ccgtcgatag aagctgcgcc gatactgtct ttacgttccg acaggatttc caaagtctgt
      301 ttgtaatcgc cgcgcggtac ggtgaaagaa aaatcggttg tgccttcgct gccgacattt
      361 tggataatca tatcgacttc gatgttggca tctgcgaccg cgcccaaaat ctgataggcg
      421 acgccgggct tatcgggcac accgcgcacg ttgatgcggg cttggttttt atcgaatgcg
      481 ataccggtta cggcagctct ttccatgttg tcgtcctctt caaaggtaat taaggtgccg
      541 ttgccgccgt cttgcaggct gctcagtacg cgcaggcgca ctttgtattt tccggcgaat
      601 tctactgaac ggatttgcaa aactttcgaa ccgaggctgg ccagttcgat catttcttca
      661 aatgtaaccg tatccatacg gcgcgcttcg ggtacgacgc gggggtcggt ggtgtaaacg
      721 ccgtctacgt cggtatagat ttggcattcg tccgctttga gggctgcggc aagcgcaacg
      781 gcggaagtgt cggaaccgcc gcgtcctagc gtggaaatat tgccttcact gctgatgcct
      841 tggaagccgg caacgatgac gactttgccg gcggcgaggt cggcgcgcat tttttcgtca
      901 tcaatgcttt ctatgcgggc tttggtgtgg gcggtatcgg ttttgaggga gacctgccag
      961 cctgtgtagc ttttggcatc cacgccgata tccttcaatg ccattgccaa aaggccgatg
     1021 gttacttgtt cgccggtagc taagacgacg tccagctcgc gcggatcggg atgctcctgc
     1081 atttcgtgcg ccaacgcaac cagacggttg gtttcgccgc tcatggcgga tacgacgacg
     1141 acgatatcgt gtccttcggc gcgggcttta gcgacacgtt tggccacgtt tttaatgcgc
     1201 tcgggcgagc ctacggatgt gccgccgtat ttatgtacga ttaacgccat gtttcatgct
     1261 ttcttgtgtg ggattggtcg ggcagcatgg tttgctggaa aagcggctat tattactatt
     1321 ttttacatga agttcaagaa cggactgcgc tttcccgcct gccgtttgac agcggtcagc
     1381 gaaaaacctg ttctttcaga tttttgacaa aatgccgtct gaacggtttt cagacggcat
     1441 acggacagta attaagcaac ggacaacgca ttttgctgat aacgcagcag ttcgcctatg
     1501 cctttttgcg ccagtgcaac cagtttgccc aactcgtcca aactgaatgg cgcgccttcc
     1561 gccgttccct gtatttcgat gattttcccc gatgcggtca tgacgatgtt cacatcactg
     1621 tcgcagccgg agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt cactacgccc
     1681 gctgatacgg cggcaacagc ttcgaggatg gggttttcac tcaaaatgcc gtctgaaacc
     1741 agtttgccga cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc cgtacgcgtg
     1801 ccgccgtccg cctgaatcac atcgcaatca atcaggattt gtcgttcgcc gagtttttcc
     1861 atatccacga ccgcgcgcag cgaacgcccg atcaagcgtt ggatttcttg tgtgcgtccc
     1921 gactgtttgc ccgccgaagc ttcgcggcgc atacgcaaag cggttgaggc aggcagcata
     1981 ccgtattccg ccgttaccca gccttggttt ttgccgtgca gaaacggtgg ggcattttcg
     2041 tccacggaag cggtacaaat cactttggta ttaccgcatt caataaggca cgaaccgtcc
     2101 gtatgcggca ggaaatgagg ggtgattttg atatcgcgca ggctgtcggc ggcgcgtgag
     2161 gtgcggatat aatcaggcat actgccctcc cgttaaaaac agataaatta aaaagcctta
     2221 aatatgaaaa atcacattta aggccttcaa actgaaaatt tctacgcctc ttcggctttg
     2281 ctacggataa tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc gaaactgccc
     2341 atcaaaaggt gcatcagccc ggtgcgcccg tgcgtgccca acaccagcag gccggcaccg
     2401 ttttcatcgg cataatcgac caaatcctgc gccatttcgc gcgcaccctt attggcaacc
     2461 agcaggtgtt tgacggtatt tcccacaccc agttcccggg cggtgcgctc ggcggcatcc
     2521 aaaacctcgt tgccttgcgc gacggcggcg gcctcgtaac tttcgtgttg caaaaattcg
     2581 ggggcgagcg ccatatattc ggcagggttg gcaacgtgta ccaaagtcag acgcgcaccg
     2641 ttgaccccgg caagctcggc ggcgtgtttc agggcattga tggacgtttc actgccgtca
     2701 acggcaacga ccagatgttt gtacatatcg tattctcctt ttgcaccgtc tcacggtgcc
     2761 gtctcttcag gtttgcgcaa aggcagtctg cgccgtttca ttatagaccc gccgtcgggc
     2821 tttatacaac agccgaacag cctgactgct ttccagtata atatgccgct ttcgtgcagt
     2881 caggcatttt tttgccggct ttcgttcact ttttgatttg acgcaatctt gcaggattcg
     2941 accatgtccg acaacgcttt gacctcttcg cgacgcttcg gcggcatcgc cagactctac
     3001 ggagactctg ccttgacgca cttttcacag gcacacgtct gcgtggtcgg cgtgggcggg
     3061 gtcggctcgt gggcggtcga ggctttggcg cgcacgggca tcgggcgttt gactttgatt
     3121 gatttggaca acgttgccga atcgaatgtc aaccgccagc tgcacgccct gaccggcgac
     3181 ttcggcaaag caaaagttac cgccttgcgc gaacgtatta cacaaatcaa tccgcaatgc
     3241 gaagtatttg aaattgaaga tttcgttacc gaagacaatt tgccggaata cttcggaaaa
     3301 ggttttgatt tcgtcatcga cgcgatcgac caagtgcgcg tcaaagcggc aatggcggct
     3361 tattttgtgg aacgcaaaca accgtttgtc ctcagcggcg gcgcgggcgg acagaaaaat
     3421 cccgccctga ttcaaaccgc cgatttaagc cgcgtaaccc acgacccgct gcttgccaac
     3481 ctgcgctaca ccttgcggaa acgctacggc ttcagccgcg atactaagga aaaaatgcgc
     3541 gtgccgtgcg tgtattcgac cgaaaatatc atgccgccgc aatcgggcgc agcgtgttct
     3601 gccgatgccg cgccgcaggg cttgtcatgc gccggctatg gcgcaagtat gctcgttacc
     3661 gcttcgttcg gactgtattg cgcacaggcg gcggtggaac acatcgcggg caaaaaataa
     3721 gcaatgccgt ctgaaacagg attcagacgg catttgaaca aacgcggcaa tcaagctgtc
     3781 ggttcagaaa aaccaacatc ggcacgccta aaaccgtatt cacaatctac tccttttcaa
     3841 aatatttgca tttaaaagcc gttataatgc cgtccgaaca tctgcccgac cacattatgc
     3901 gtgaatgccg gcagattgtt ttcttttgta aacttatatt aaaattcact taccaattca
     3961 cgccatgccg cccatccctg ccctatccgc accatccgag cacactgtcg catgggtatt
     4021 cggccaaccc gttaccgatt tgccgcagga tttgtttatc ccgcccgacg cgctgaaagt
     4081 cgtattgggc agttttcaag gccctttgga tctgctgctc taccttatcc gcaagcagaa
     4141 catcgatgtt ctcgatattc cgatggtgga aattaccggg cagtatctgc actatattgc
     4201 ccaaatggaa gcctatcagt ttgatttggc ggcggaatat cttttgatgg cggcaatgct
     4261 gattgaaatc aaatcgcgcc tgctgctgcc gcgtaccgaa gccgtcgaag acgaagaggc
     4321 cgacccgcgt gccgagttgg tgcgccgtct gcttgcctac gagcaaatga aactggcggc
     4381 gcagggtttg gacgcgctgc cgcgtgcggg acgggatttc gcgtgggctt acctgccgct
     4441 ggaaattgca gccgagacga agctgcccga ggtttacatc gccgatttga tgcaggcatg
     4501 gttgggcatt ctttctcggg caaaacatac gcgcagccac gaagtaatcc aagaaaccct
     4561 ttccgtgcgc gcgcaaatga cggcaatcct gcgccgtttg aacgaacacg ggatatgcag
     4621 gtttcacgac ctgttcaatc ccgaacaggg cgcggcttac gtgatcgtca acttcatcgc
     4681 cctgttggag cttgccaaag aaggattggt cggaatcgta caggaagacg gtttcggaga
     4741 aatccgaatc agcctcaatc atgagggggc gcattcagac ggcattttcg gcacacgggg
     4801 cgggcgcgat gtgttctaat acgccccaag ccgccaccag aaatcgggag acacgccata
     4861 tgaccggcat catacattcg ctgctcgaca ctgacctcta caaattcact atgctgcaag
     4921 tagtcctgca ccagtttccg cagacgcaca gcctttacga attccgctgc cgcaacgtct
     4981 cgaccgtcta tccgcttgcc gacatcaggg aggacttgga agccgaactc gacgcgctct
     5041 gccggctgcg cttcacccac gacgaactcg gctatctgcg ctccctgcgt ttcatcaaaa
     5101 gcgactttgt cgattatctc gaactcttcc agctccaacg ccgctttgtc gaagtcggca
     5161 cagacgataa aggccgtctg aacatccgta tcgaaggccc gatgatacag gcgatgtttt
     5221 ttgaaatctt catcctcgcc attgtcaacg aactttactt ccgccggctg gaaacccctg
     5281 ccgttatcga agaaggcgaa cgccggcttc aagccaaagc cgcgcgcctg aaagaaatcg
     5341 ccgccgcaca gaatccggac gaaccgccct tcctgatttc cgacttcggc acgcgccgcc
     5401 gctacaagct cgcgtggcag gaacacgtca tccgcaccct gctcgaagcc gccccaagca
     5461 tcgtgcgcgg cacaagcaac gtttacctcg ccaaaaaact cggcatcacc cccatcggca
     5521 ccatggcgca cgaattcctc caagccttcc aagccctcga cgtgcgcctg cggaatttcc
     5581 aaaaggccgc gctcgaaagc tgggtgcacg aataccgggg cgatttgggc gttgccctga
     5641 ccgacgtggt cggtatggat gccttcctgc gcgatttcga cctctatttc gccaaactct
     5701 tcgacgggct gcgccacgac agcggcgacc cttacgtttg gggcgacaaa gcctacgccc
     5761 actatcaaaa gctcaaaatc gacagccgca ccaaaatgct gaccttctcc gacgggctgg
     5821 acatcgaacg ctcttgggca ttgcaccaat atttcaaagg ccgcttcaaa accggcttcg
     5881 gcatcggcac caacctcacc aacgatatgg ggcatacgcc cttgaatatc gtgttgaaac
     5941 tggtcgaatg caacgggcag tccgtcgcca agctctccga ctcgccgggc aaaaccatga
     6001 ccaacaacag caccttcctc gcctacctgc gccaagtgtt cggcataccc gaacctagaa
     6061 cgccgtaaac cggcagaaaa agcgtacaat tcctgtttcc gccgcataaa atcttttaaa
     6121 ataccgcctg atttgaattt aaccgaaaga ccgaacttca tgaacctaca tcaaaccgtc
     6181 gaacacgaag ccgccgccgc ctttgccgcc gcaggcatcg ccggcagccc cgttgtcttg
     6241 cagccgacca aaaatgccga acacggcgat ttccaaatca acggcgtgat gggtgcggcg
     6301 aaaaaagcca aacaaaatcc gcgcgaactg gcgcaaaagg tcgccgacgc attggcgggc
     6361 aacgccgtca tcgaaagcgc ggaagtggcc ggacccggct ttatcaacct gcgcctgcgc
     6421 cacgaatttc tcgctcaaaa cattcatgcg gctttgaacg acgcgcgttt cggcgtagcg
     6481 aaaactgccc aaccgcaaac cgtcgttatc gactattctt cgcctaatct ggcgaaggaa
     6541 atgcacgtcg gccatctgcg ctccagcatc attggcgaca gcatttcacg cgtgttggaa
     6601 tttacgggca acaccgtcat ccgccaaaac catgtcggcg actggggtac gcagttcggc
     6661 atgttggttg cttatttggt cgagcagcag aaagacaatg ccgcgttcga gctggcggat
     6721 ttggagcagt tttaccgcgc cgccaaagtg cgctttgacg aagaccctgc ctttgccgac
     6781 accgcacgcg aatacgttgt gaagctgcaa ggcggcgatg aaaccgtgtt ggcgttgtgg
     6841 aaacagtttg tcgatatttc gctctcgcac gcccaagccg tttacgacac gctgggcttg
     6901 aagctgcgcc ccgaagatgt ggcgggcgaa tcgaaataca acgacgattt gcagcccgta
     6961 gccgatgatt tggttcaaaa aggcctggcg gttgaggacg acggcgcgaa agtcgtgttc
     7021 ttggacgagt tcaaaaacaa agagggcgaa cctgccgcat ttatcgtgca aaaacaaggc
     7081 ggcggcttcc tttatgcttc caccgatttg gcgtgcctgc gctaccgcat aggccgtctg
     7141 aaagccggcc gcctgctgta cgtcgtcgac caccgccaag ccctgcactt cgagcaactc
     7201 tttaccactt cccgcaaagc aggctatctg cctgaaaatg caaaagccga gtttatcggc
     7261 ttcggcacca tgatgggcaa agacggcaaa ccgttcaaaa cgcgcagcgg cgacaccgta
     7321 aaactggttg acctgctgac cgaagccgtc gagcgcgcca ccgctttggt gaaagaaaaa
     7381 aatcccgaat tgggcgcgga tgaagccgcc aaaatcggca agaccgtcgg catcggcgca
     7441 gtcaaatacg ccgatttaag caaaaaccgc accagcgatt atgtgttcga ctgggacgcc
     7501 atgctctcat ttgaaggcaa caccgccccc tacctgcaat acgcctacac ccgcgtgcaa
     7561 agcgtgttcc gcaaagccgg agaatgggac gcaaccgcac cgaccgtttt gaccgaacca
     7621 ctggaaaaac agcttgccgc cgagcttctg aaatttgaaa acgtgctgca aagcgtggcg
     7681 gacaccgcgt atccgcacta cctcgccgcc tacctttacc aagccgccac cctgttcagc
     7741 cgcttctacg aagcttgccc gattttgaaa gccgaaggcg caagccgcaa cagccgcctg
     7801 caactggcaa aacttaccgg caacacgctg aaacaaggct tggatttgct gggcatcgat
     7861 gtgttggacg taatgtaaac accgcaccgc ccgccatcct catccgaatc tgaaaaaagc
     7921 ggcgcgatac accgtatccg ccgcctttcc caaaatgcga aacaaacaaa cgccaagcaa
     7981 gcaagcaagc aagcaagcaa gcaagcaagc aaaaattata gcccctttcc tgccggcgca
     8041 cacactttcc gcgcggcaca tccccctttt tccgcccctc aaacccgcct tttcttcagg
     8101 cagggtttca gcccgcctcc tttccctgtt ttcctttccc cgacacgcgt gcgcgcccct
     8161 gccgcaccgt gccgcgcttt tgcgcccgga cggcatcgtt ccgccatctg gttttctgtt
     8221 ttaaataccg ctgtttcaga aagaaatgca gatgtttcga cacacaggac gacatataaa
     8281 gtaccgccct atgtgtcgtc ctgatttgga aggggttaca cccctcccaa ccagataaag
     8341 tctgatcctg ccgccccgaa ggacggatgt ccaagtggcg gggtttcaac cgaaaaggaa
     8401 atacgatgaa tattcacgcc ctgctctcca aacaatggac gctgccgcca ttcctgccga
     8461 aacggctgct gctgtccctg ctgatactgc tggtccccaa tgcggtgttt tgggttttgg
     8521 cactgctgac cgccaccgcc cgcccgattg tcaatttgga ctaccttccc gccgcgctgc
     8581 tgatcgccct gccttggcgt ttcgtcaaaa ttgccggcgt attggcgttt tggctggcgg
     8641 ttttgtttga cgggctgatg atggtgatcc aactcttccc ttttatggac ctcatcggcg
     8701 ccatcaacct cgtccccttc atcctgaccg cccccgcccc ttatcagata atgaccgggc
     8761 tgttgctgct gtatatgctg gcgatgccgt ttgtgttgca aaaagccgcc gtcaaaaccg
     8821 acttccgaca cattgccgtc tgtgccgccg ttgtggcggc agccggctat ttcaccggcc
     8881 atttgagtta ctacgaccgg gggcggatgg ccaatatctt cggcgcaaac aacttctatt
     8941 acgccaaaag tcaggcgatg ctctacaccg tcagccagaa tgccgacttt attaccgccg
     9001 gcctggtcga ccccgtcttc ctccccttgg gcaatcagca gcgtgccgcc acgcggctga
     9061 gtgagccgaa atctcaaaaa atcctcttta tcgtcgccga atcttggggg ctgccgggca
     9121 atcccgagct tcaaaacgcc acttttgcca aactgctggc gcaaaaagac cgtttttcgg
     9181 tttgggaaag cggcagtttt cccttcatcg gcgcgacggt cgaaggcgaa atgcgcgaat
     9241 tgtgcgccta cggcggtttg cgcgggttcg cactgcgccg cgcacccgac gaaaaatttg
     9301 cccgctgcct ccccaaccgt ttgaaacaag aaggttacgc cacctttgcg atgcacggcg
     9361 cgggtagttc gctttacgac cgcttcagct ggtatccgag ggcgggcttt caaaaaatca
     9421 aaaccgccga aaacctgatc ggtaaaaaaa cctgcgccat tttcggcggc gtgtgcgaca
     9481 gcgagctgtt cggcgaagtg tcggcatttt tcaaaaaaca cgacaaggga ctgttttact
     9541 ggatgacgct gaccagccac gccgactatc ccgaatccga cattttcaac cacaggctca
     9601 aatgcaccga atacggcctg cccgccgaaa ccgacctctg ccgcaatttc agcctgcaca
     9661 cccaattctt cgaccaactg gcggatttga tccgacgccc cgaaatgaaa ggcacggaag
     9721 tcatcatcgt cggcgaccat ccgccgcccg tcggcaacct caatgaaacc ttccgctacc
     9781 tcaaacaggg acacgtcgcc tggctgcact tcaaaatcaa ataacaacaa tgccgtctga
     9841 acgcaccaac agccttcaga cggcattttg cagacagacc gacctcctca agcccacttt
     9901 tttcatcatc tccgataaat tgctttgtaa gaatccaaaa atccaaaatc acaggaagtt
     9961 atcaaaaaaa cagaaaccat ccgccgtcgt tcccgcgaaa gcgggaatcc agacccggaa
    10021 accgcagaaa tttatcggaa aaaccgaaac tcgaaaacaa cggacagaat tcccgcctgc
    10081 gcaggaatga cggctcatac atcacccaaa aaaaattgaa accaaacagc tgggattccc
    10141 tcttgcgtga ggctgacaga tgccgtctga aagactttca gacggcatag ggcgttttct
    10201 ctttgaattt aaattttgcc acaaaaaagc cgccccaaat gaatacccgg gcagcttttt
    10261 gttgatatga ctccaatcag cggtgttgcg gattgtaacg tttttccaaa cgcaggaata
    10321 tccagcctaa gaaagtcgtc atcaacagat aaatcagggc ggcggtgtaa agcggttctt
    10381 cataaaccga ataccggccc gtaatcgtat tctgaacata cgccaactcc gccacagcaa
    10441 tgaccgacag cagcgagctg tctttcaaga gcgtgatgaa ctcgctcgcc aaaggcggca
    10501 gcatacggcg caatgcctgc ggcagaatca catagcgcat cgcctgcgga taggtcagtc
    10561 ccaaagaaca cgccgcctcc atctgtcctt tgtctataga ctggatgccc gcgcggaaaa
    10621 tctcacagat atacgccccc gagttggcga tcagtgccaa agaaccggca atcagcggcc
    10681 cgtatccgcg acgcagcgcg attgccgcct cgccgctgac caaaatgccg tctgaaggat
    10741 ggacgaaaaa cggaaaccac acatacgccc aaatcacaat ctgcacaaac agcggcgtac
    10801 cccggaacag ggtaacgtac agcagcgaaa ccttacgcaa cgcccacgcc agcacgcgca
    10861 tcggcgcacc ggctttttcc aagtgaatca ggcgcgccaa cgccaacaac agacccaata
    10921 ccgaaccgcc cgccgtcgcc acgaccgtca gccccaaggt cgtcagtgcg ccgtaaagaa
    10981 acatccagcg gtattcgtaa ataatgtcaa aacgaaaatc cataaaccgt ccgtatcaaa
    11041 aaccggcgga actgccgccg ttgcaaaata atccgccatt ttaccgtaaa aaccgccgcc
    11101 tgaacttttt tatcgcggca gacggcggtt gcgcgtctcc gcaaaaatgc agggcgcgcg
    11161 gttttcagac ggcatttgcc gttcaaggcc gtgcggtgtc tttaccaaat gcccaaccat
    11221 tcgcccacgg aatccatcca atccttattg cccccgccgc tcctgcctgc ccggcggtac
    11281 gcccacggcg cttgcggatt tttagctttc cacaatcctt tgcgttccct ttccgcctga
    11341 atttgagcgt cggcatagtc ggcaaaatcc gccttatcct gctgttcttt agcataactt
    11401 ttataatgcc acgccgcccc gtcctgcacc tgcatcaggt tcaaatcggt tttgccggcg
    11461 gatacctgcg ccacttcgcg ctgatagcgg tcggtttcaa acacacgtac actgactttc
    11521 ctaccctccg ccgccgcgcg caggttgtcg cgcgaacgtg taccgtaagc ctgtttcatc
    11581 tccggtgcgt cgatatacgc catccgaatt ttatgtttcg cgccgtcgcc gtcgatgacg
    11641 tgaagggtat cgccgtcata gactttggac accgtgcctg tgtagctgtg gccggatttc
    11701 gccgatgccc gtcggcgaac gggcgcgtcg gaacccacgt cccctgccgt gccgagtacg
    11761 tcgagtacgg caaccgccgt ccgcaccgcc tcactgtcat atcccgtata acccaacgcg
    11821 cccaaaagcg acagggcgac gggaagccat ttcatgattt ttttaatctg catatttttc
    11881 aaatgccgat gccgtctgaa catctctgaa ccggatttca gacggcatct taacgtcagg
    11941 attacccttg gcagggatag atgactttcg caccctcttc cgtccccaaa atcaacacat
    12001 cggcggcatc gcgggcgaat atgccgtttt cgagtacgcc ggtgattttg ttgatttcat
    12061 cttccatcgt cagcggctga tcgatattca agccgtggac atcgacgatt tggttgccgt
    12121 aaaacgtggt gtagccgata cgcagttcgg gctgtccgcc catggcaagc agtttgcgcg
    12181 aaacaaggga gcgcgcgctt tcgacggctt ccacgggcag tgggaatttg cccagccggg
    12241 aaacatattt gctttcgtcc gcaatgcaga cgaatttttc tgatgcgctg gcgacgattt
    12301 tttcgttgag gtgcgcgccg ccgccgccct taatcatttg cagggcgtgg ttcacttcgt
    12361 ccgcaccgtc gatatagacc gccaaaccgg acacttcgtt cagggaaacg acgggaatgt
    12421 cgtaccgggc aagcagttcg ccggattttt tggaggttga aaccgcgcct ttgatttttt
    12481 taccgctttt gcccaaggct tcgatgaaga aattgatggt cgaaccggta ccgatgccga
    12541 tgtattcgtt ttcgggtacg aattcgactg ctttttcggc ggcgatgcgc ttgagttcgt
    12601 cttgtgtcgt catattttta tcctttggga aaccgtatca acaaacagcc gccatcttaa
    12661 catttttttg cacgtcctgc ccgccgcgtt caaatccgta ccagcaatac cgccgcctgc
    12721 gcctctatgc cttccatccg cccgagatag ccgagttttt cgttggtttt gcctttgata
    12781 ttgacgcacg aaatatcgat acccaaatcg gcggcgatgt tggcacgcat ttgcggaatg
    12841 tgcggtgcga gtttgggttt ctgtgcaatc acggtcatat cgacattgac cgcctgccaa
    12901 ccctgcgcct gaacgctttg atacgccgca cgcaaaagga cgcggctgtc cgcatctttg
    12961 aactctgcgg cggtgtcggg aaaatggctg ccgatgtcgc ccaaacctgc cgcaccgagc
    13021 agcgcgtcgg taacggcgtg cagcagcgcg tcggcatcgg aatgcccgag cagccctttt
    13081 tcaaatggga tttcaactcc gccaagtatc agctttctgc cttcggtcag ttggtggaca
    13141 tcgtagccct gtccgatacg gatattcgtc atcgtttgtg ttcctgatgt tttgaattga
    13201 atttcagacg gcattgagca gcagcctgac gatgtatgcg tcctgcggct gcgtcagttt
    13261 caaattgcgc gcgtcgccct gtatcagtag cggacgcaca cccagttttt ccacggcgga
    13321 cgcttcatcg gtaatgccgc ccaagttttc cgcagccaat gcgcggtgca gcaaacccgc
    13381 ttgaaaaagc tgcggcgttt gcgcctgcca aagccccgaa cggtcgacag ttgcactgat
    13441 ttgtccgctt tctgcgcgct tgatcgtatc ggcaacggga actgccaaaa tcccgccttc
    13501 ggcggcgttg cccgcctgtt ctatcaaccg cgtcagagct tcagacggca ggcagcagcg
    13561 ggcggcatcg tgtaccagaa tattgtcggt ttccgccgcc aaaccggttt ccaacagttt
    13621 tgccacaccg ttgcggacag tttcggcgcg ggtctgtcca ccgtttttcc acacccgaac
    13681 ctgtggaaat gccgtctgaa ccttatcggc aaacgtgtct tcgggcgaga cgacaacgac
    13741 ggtcaaatcg acggcctcat gccgttcaaa aatcccaagt acatgttcta aaacggtttt
    13801 gcttccgatt tcgacatatt gcttgggttt gtccgcaccg aaacgcaccc cgatgccggc
    13861 ggcgggaatc agcgcgatat ttttgcgttt catgcgtttt ccccgccgtt ttcagacggc
    13921 acggcttcct tacgccaaat acaggcttcg cccaagccgt cgagatattc ttcgtgcgcc
    13981 gccagttcgg cttcatccgc cttaatcact ttcagtttgg caccgagttt catttcggca
    14041 gatgcaacag gtttggctgt cggttcttcc tcctcggtct cccccatcaa gtcgaattgc
    14101 cgccgcgtca tcgccagata gacttcgccc aaaagttcgc agtcgatcaa tgcgccgtgc
    14161 aggacgcgct tgctgcggtc gacggaaaaa cggttgcaca gggcatccag actggctttc
    14221 tgcccgggga acatttcgcg cgccatcgcc agggtatcgg taacggtgca gccgagttcc
    14281 tcgacggtcg gcagccccat ccggcggaac tccatattga ggaagcccac atcgaatttg
    14341 gcattgtgga taatcagttc cgcaccgcgc agaaaatcgg caatctgcct gccgacctct
    14401 gcaaacggcg gcgcgttttt ctcttccaaa acctgtatcg tcaagccgtg gacgcgtgcc
    14461 gcctcttcgg gcatatcgcg ctcggggtgg acatagaggt gcaggttttt gtcggtcatt
    14521 tggcggttga ccatttccag accggcaaac tcgaccagac ggtcgccgcc gtcggcatac
    14581 aggccggtgg tttcggtatc gaggatgatt tggcgtgtca tcatatcggt gtctttcttc
    14641 tgtcttcgta aattgattat tttttaagca atgtattttt ctgttttcat ttcaatgcac
    14701 aaacccactt attcacagtg tgttcacaac attgggcagg cggattgtgt attttgggga
    14761 caatttttcc agacggcatt caaggttttt tcctgattgc cgccgcgcct aaaaaccgcc
    14821 tttcgcgctt aatcaaaaat accgccaacg gaatattgcc caaggcgaca atcagataca
    14881 gcagggaaat gctgtcaaac aaaaacagca ataccgcgct caaaacggcg gcggaaacca
    14941 taaagatgcc gttaacgata ttgttggcgg caacggcgcg ggcgcggaaa gtctcgctgc
    15001 tggcggtttg cagccaggta tagagcggaa cggagaaaaa tccgccgaaa aagccgatca
    15061 gcgtcatcac cgccatcacg gggtatgccc atccttgcga taaaaaccaa aaaatgccgt
    15121 tcagcccttc aaaacggtgt ccgtgcgtca gccacaccaa aaccaggccg caaaccgtca
    15181 aacccaacgc accaaccgtt acccaagcca acatcagccg ttccctgccg aacttggcac
    15241 acagtaccga accggcggca ataccgatgg aaaacaaagc aagcatcagg ttaaaaacat
    15301 tatcgttgcc gcccaaacgg atttgggtaa aggtcggcag ttgcgtggta taaaccgcgc
    15361 cgacaaacca aaaccacgag atgccgataa tggcggtaaa aacgggattg tgccgcaccg
    15421 tttcacgcag cagggatttt gtaccacgga caatattcca ctcgatttgg gtatcggcag
    15481 ccttggcggg tacggacggc ataaacaggc tgccggccgt tcctccgacg gcgaccagca
    15541 aaaccagtat cccgacaata taaggcggcg cgccggcaac cgccgttccc aaaatctgac
    15601 caaacaggat ggcgacaaac gtaccggatt caatcaggct gttgcccatc atcagctcgt
    15661 tgtcgtcgag ataatcgggc agaatggcat atttaagcgg gccgaacagc gtcgattgcg
    15721 cgcccatgca aaacaggcag agcaagagca gcggggctga ccggatataa aacccgtatg
    15781 ccgccaccgc cataatgatg atttccagca ccttgaccca acgcgccaaa acggccttgt
    15841 cgaatttgtt acccaactgc cccgacagcg cggaaaacag gaaatacggc aaaataaaca
    15901 gcagtgcgcc caagttcaac atctgtccgg caggcagaac gccgttttgc cccaaaccgt
    15961 aaaacccgat catcacaaac agcgcggttt tgaacacatt gtcattgaac gcgccgagaa
    16021 actgcgtagc gaaaagaggc ccgaaacggc ggcttttaac cagtcccaaa ccgccttttt
    16081 tagcgtccat cgttttcctt ctctttttca atcagtttac ttgtcgaatc atcatccatc
    16141 aaaatgcggt gcgccggtcc ttccaaatcg tcaaactgtc cgtttttgcc cgaccaccag
    16201 aaaaaccagc cgatgacaaa tgccaaaata atgctgatgg gcaccagaat aaacatactt
    16261 tccatcacat attccctatc aaatcgttca aaacaaaagt ctgccctgac acggtcagat
    16321 attcgttacg caaagttccg acgggggctt cgtcaaaaaa cagctcgata cggtctttga
    16381 ccacgcgcca atattgggga atttccgtct gaccgaacgg cgacaggaca tggttgcccg
    16441 tcccatcttc caatttaacg gcaaaacgcc cgctctgcag ccgtgcttcc gattcgtcgt
    16501 cggcaagaag gatgaaaaag cctatatgcc gtcccgattg gtcatgaata ctgaaataat
    16561 gcataaattc cccacccgcc ttttttcaga cggcatcaac taaaaacagg gcgaatgtac
    16621 cagtttggac gggaaaaatg caaagaaatt ctccctcccc cagccgaaaa caccggcaaa
    16681 ccgcatatcc cccttttttc cgtcaaaata cccgacttcc gccattttca cgcaaacgcc
    16741 cgattaagcc aagcaattgc aaagattttt tgctagaata gcctgcttct tttatcaacc
    16801 gtttcagacg gctccactac tttcccgtca aggaaagcaa agcggattcg gcacgaatcc
    16861 gattagtatc cgtgtccgat tccaatgccg tctgaaactt tccggagtaa gaaaatgtcc
    16921 caaaaattga tcttggtttt gaactgcggc agctcgtccc tcaaaggcgc ggtcctggat
    16981 aacggcagcg gcgaagtcct gctgagctgc cttgccgaaa aactcaacct gcccgatgcc
    17041 tacattacat tcaaagtaaa cggcgaaaaa cacaaagtcg atctgtccgc acaccccgac
    17101 cacaccggcg cggtcgaagc cctgatggaa gaactcgaag cccacggcct cgacagccgc
    17161 atcggcgcca tcggccaccg cgtcgtcagc ggcggcgaac tgtacaacga atccatcctc
    17221 gttgacgacg aagtcatcgc cggcatcgaa aaatgcatcc cgctggctcc tctgcacaat
    17281 cccgcccacc tcttgggcct gcgtgccgcg caaagcattt tcaaaggcct gcccaacgtc
    17341 gtcgtattcg atacctcctt ccaccaaacc atgcccgaag tcgcctacaa atacgccgtt
    17401 ccgcaggagt tgtatgaaaa atacggcctg cgccgctacg gcgcgcacgg taccagctac
    17461 cgcttcgttg ccgacgaaac cgcgcacttc ctcggcaaag acaaaaaaga cctgcgtatg
    17521 gtcattgccc acttgggcaa cggcgcatcc atcaccgccg tcgccaacgg agaatcgcgc
    17581 gacaccagta tgggcctgac cccgctggaa gggctggtaa tgggtacgcg cagcggcgac
    17641 atcgatcctt ccgtattcgg cttcctcgcc gaaaacgcca atatgaccat tgcccaaatc
    17701 accgacatgc tgaacaaaaa atccggcctg ctcggcattt ccggcctgtc caacgactgc
    17761 cgcaccattg aagaagaagc cgccaagggg cataaaggcg cgaaattggc cttggatatg
    17821 tttatctacc gccttgccaa atacatcggc agtatggcgg ttgccgcagg cggcttggac
    17881 gcactggtct ttaccggcgg tatcggcgaa aactccgaca tcatccgcga acgcgtaatc
    17941 ggctacttgg gcttcctcgg tctgaacatc gaccaagaag ccaacctgaa agcccgcttc
    18001 ggcaacgccg gcgtgattac cactgccgac agcaaagccg ttgccgtggt cattccgacc
    18061 aacgaagagc tgatgattgc ccacgatacc gcccgtttga gcggtctgta aggttttatc
    18121 ctcacgcgaa ctgcctccgg aaacggaggc agttttttat agtggattaa atttaaaccg
    18181 gtacagcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg tcgccttgtc
    18241 ctgatttttg ttaatccact atatccggct ttccatgctt aaacagcact acctcttttc
    18301 agacattgac ggttgcagcc gcctacctga accttaatga ttgactattt tttaatcatg
    18361 ttattatttt ccataaaata catggcatta agatgttttt ccacaaaaga tacacacacc
    18421 ggcaaacacc ggctgtgttt atcttttctt atgcctattt tttaatcatc gtatttttat
    18481 cttttaattt caatacgcaa accgacttat acacacggtt ttcacatctt tggactgctt
    18541 ccgtgtgtat agcggatatt gccgttttcc tatctgacaa aaatgccgtc tgagaacttc
    18601 agacggcatt tgaaacatcg gaatcagcgg ttttgttcat accactcgat aaacttgtct
    18661 gctttgacaa aacccagcag cggctcgctg cggctgccgt cggcgcggac gacaaacacg
    18721 cccggcggcc cgaacagacc gtattctttc aacaacgcct gatgttcggg cgtgttggcg
    18781 gttacgtcga tctggaaaaa gcgttccata tcgactgcct gatgcacttc cggctgattg
    18841 agcgtgtaaa ccgccatttc tttgcaggaa atgcaccagt cggcataaaa atccaaaacg
    18901 acaggtttgt cgggatgttc tttcaacgcc gtatccatcg ctgccttcag cgcggcagta
    18961 tcggcaaaca ttttgccgtg ttccgaaaat ttgcctgctt cggccggcgg attgagggtc
    19021 aagaaatggt gcagcgcggt cgttttgccg tttgcgccct gccagccgaa ccacgcgccg
    19081 cctatcagca atatgctgcc caatgcgaat gccacagctt tcggacggcg tttctgcctg
    19141 cgtccgttga ccagcagcat aaaggcagga accagcatca gcagcgtgta cagcgcgacg
    19201 acgagataat agggcaaatg cggcgtggcg aggtaaacgg cgacggcaag caggataaag
    19261 ccgaaagcgt atttgacggc attcatccaa tcgcccgcct taggcaggat atgtcctccg
    19321 aacgtgccga tggcaatcag cggcacgccg gtgcccaacg ccaaagtgta aagtgccaaa
    19381 ccgcccaaaa ccgcatcgcc cgtctgaccg atatagccca aggcaaacgc cagcggcggg
    19441 gcgacgcacg gtccgacaat cagtgcggac agaataccca taataaagac ggaaacgatt
    19501 ttaccgccgg agagcctgct gctctggttt tggaaatatg actgtacggc gttgggaagc
    19561 tggatgttga acagcccgaa catagacagc gccaagacga ccatcaaagc cgatgccgcc
    19621 aacacgaccc aagcctgctg caaccatacg gtcagcagcg cgcccgtcag tccggcaaca
    19681 atgccgacca gcgtataagt cagagccaaa ccctgaacat aaacgacgga cagcacaaac
    19741 gcccgcgcct tgcccgcctt tttgtcaccg accacgatac tggacacaat cggcagcagg
    19801 ggatacatac aagcggtaaa actcaggccc aaaccggcga gaaaaaacgc caaaagattg
    19861 gcgttgagcg tatcccaaga cagcttgaaa cggccgtcgc cgccctcgtc ccccttcggg
    19921 ggtggcaacg ccccgctgcc gttttgagag gaaggctgca aaaaacggtc tttggcggat
    19981 gccggttcgt cggtttgcgg atggtaagtg ccgttgccgg aaatatcaaa ctcggtatcc
    20041 acgggcggat agcacacgcc ggcttcggca cagccctgat aggtcaaaac caatttatac
    20101 ggttcgccga cagcctttgc ataaggaaag gcaacctgcg cctcgtgatg gtaaaccgtc
    20161 tgcctgccga aaaactcgtc ttccttctct tcgcccttac tgaaagaagg ctgtcccaac
    20221 aaatccgccg gatcggtctt gccgacgatt ttcgcctgat acatatagta tccgtcggca
    20281 atcctgaaac ggacgttcac gccgtcgtcg gcaacagtaa gctccggcac gaatgccttt
    20341 tccggcggca gcagatcgtt cgcatccagc gcgaaagccc gtccgcacaa catcaaaaat
    20401 acggcgaaca ggcaaatcag ttttttcata atcgaatccg tttcagacaa ataatttgtc
    20461 cgcattataa atggtaaggt tgacggtagg atttaattta tgtaaaaccc gccattatcc
    20521 taacctattt ccataaacat cttatcggac ccgccatgta cgatgtcaat acccacgatg
    20581 tccgccgctt tttcgcccgc gtgtggcagc agcggctcaa tccgctgcaa ttgggcgcgt
    20641 tggaacagaa agccctccgc attgtcgaag cccatcccga ataccaccgt tatctcgaac
    20701 gcatcgaaga ccatctggac accgactggc tgcccgaaaa cggcgaaagc aaccccttcc
    20761 tgcatatgtc gctgcatttg tccgtccaag aacaggcggg catagaccag ccgcacggca
    20821 tacgcgccat ccacgacacc ctgtgcgcca aacgcggctg gccggaagcc gaacacgaaa
    20881 tgatggaggc actggcggaa acactgtgga cggcgcaacg ctacggcacc ggtttggatg
    20941 tcaatttcta catgacccga ctgcgcaaac tcatcggctt gggtgcagag gatcaagcca
    21001 ggttaaaccc gcatgaaatc gcctgaccat accgaccgcc tgcaaaatgc cgtctgaagc
    21061 ggaacaaccc ctttcagacg gcattcattt tcccccaatc atttccacaa cgcgcgtttc
    21121 aacataatca accaatcctt cttatccaaa acggggcgtt gtgcaaacac atcgtatcgg
    21181 cacgcgtcca gtttctgcaa aatcaactgc gcccccaaca caatcatacg gagttccaaa
    21241 ccgatacgcc ctttcaattc gcgcgccaaa ggcgaacccg ccttcagcat acggaatgca
    21301 cgccggcact catacgccat cagccgctga aacgccgcat ccgcccgtcc tgctgcgatc
    21361 tgttcctcag aaacaccgaa tttcaacaaa tcgtcctgcg ggatataaac cctgcccttt
    21421 tgccaatcca cagctacatc ctgccaaaaa ttcaccagtt gcaaagccgt acaaataccg
    21481 tcgctttgcg ctacgcacac cgcatccgtt ttcccgtata aagccagcat aatgcgtccg
    21541 acagggttgg cggaacgccg gcaataatcg gtcagatcgc cgaaatgcgc gtaccgcgtt
    21601 ttaaccacat cctgcgaaaa cgccgagagc agatcataaa acggctgcaa atccaaaccg
    21661 aacggcacaa ccgcctcggc atccaatcgt gcaatcaaag gatgcgccga ccggccgccc
    21721 gatgccaaca cgtccaactc gcgccgcaaa ccctccaacc ccgacaacct ggcttcagac
    21781 ggcatactgc cctcgtccgc catatcgtcc gccgtccgtg caaacgcata caccgcatga
    21841 accggcttcc tcaacctgcg cggcaaaacc agcgaaccga cgggaaaatt ctcataatgc
    21901 ccaaccgaca taccttctcc atccatcaaa caaaatgccg tctgaaacgg aacaaaccct
    21961 tttcagacgg catcagatac ctccaagctg ccggcaatca gtggtggtaa tgaccgtgcg
    22021 aaccgtggac atgaccgtgt gcgatttcct catcggatgc atcgcgcacg ctttcaaccg
    22081 tagctttaaa gcggattttc atgcctgcca aaggatggtt gccgtccacc accgccttgc
    22141 cgtcggcaac atcggttaca cgatagacga caacatcgcc ggtttcagga tcgtcggctt
    22201 caaacatcat gccgacttcg acttcaacag ggaacgcgcc cgcatcttca atacggacca
    22261 actccgaatc ctgctcgccg aacgcatcgt cggacgaaag cgccacatcg accgtatcgc
    22321 cggcatcctt accgtgcaac gcctcttcca ccaaagggaa aatgccgtcg taaccgccgt
    22381 gcagatacgc aatcggttct tcggttttat ccaaaagctg attgttggca tcatacatct
    22441 cataatgcag cgaaaccacg gaattttttg caatagccat atttgtcctt tcaggaacag
    22501 cagattgatt acaggcgcat tctaacacaa ccgccgcgcc ggccgattac cgttaacctg
    22561 ttcataaact gtacggcaca tatttcaatg taaatttttg ttattttatt gcagtgtaaa
    22621 ttttttacaa cactctcaaa aaccattccg acccatctgc cgacttcccc aatctgcctt
    22681 aat
//
LOCUS       DAXFWM010000031        21700 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000031 DAXFWM010000000
VERSION     DAXFWM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21700
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.031"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..1310)
                     /locus_tag="ACFI8B_001123"
     CDS             complement(<1..1310)
                     /locus_tag="ACFI8B_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC3834358.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(1446..2624)
                     /gene="ftsZ"
                     /locus_tag="ACFI8B_001124"
     CDS             complement(1446..2624)
                     /gene="ftsZ"
                     /locus_tag="ACFI8B_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC3834359.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2743..3987)
                     /gene="ftsA"
                     /locus_tag="ACFI8B_001125"
     CDS             complement(2743..3987)
                     /gene="ftsA"
                     /locus_tag="ACFI8B_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC3834360.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(4073..4801)
                     /locus_tag="ACFI8B_001126"
     CDS             complement(4073..4801)
                     /locus_tag="ACFI8B_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC3834361.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(4791..5705)
                     /locus_tag="ACFI8B_001127"
     CDS             complement(4791..5705)
                     /locus_tag="ACFI8B_001127"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC3834362.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(5817..7226)
                     /gene="murC"
                     /locus_tag="ACFI8B_001128"
     CDS             complement(5817..7226)
                     /gene="murC"
                     /locus_tag="ACFI8B_001128"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC3834363.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(7381..8448)
                     /gene="murG"
                     /locus_tag="ACFI8B_001129"
     CDS             complement(7381..8448)
                     /gene="murG"
                     /locus_tag="ACFI8B_001129"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC3834364.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8452..9723)
                     /locus_tag="ACFI8B_001130"
     CDS             complement(8452..9723)
                     /locus_tag="ACFI8B_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC3834365.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRQKMRGYRVE"
     gene            complement(9902..11239)
                     /gene="murD"
                     /locus_tag="ACFI8B_001131"
     CDS             complement(9902..11239)
                     /gene="murD"
                     /locus_tag="ACFI8B_001131"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC3834366.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11324..11704)
                     /locus_tag="ACFI8B_001132"
     CDS             complement(11324..11704)
                     /locus_tag="ACFI8B_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC3834367.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(11829..12911)
                     /gene="mraY"
                     /locus_tag="ACFI8B_001133"
     CDS             complement(11829..12911)
                     /gene="mraY"
                     /locus_tag="ACFI8B_001133"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC3834368.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13156..13332)
                     /locus_tag="ACFI8B_001134"
     CDS             complement(13156..13332)
                     /locus_tag="ACFI8B_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834369.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(13335..14693)
                     /gene="murF"
                     /locus_tag="ACFI8B_001135"
     CDS             complement(13335..14693)
                     /gene="murF"
                     /locus_tag="ACFI8B_001135"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC3834370.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14818..16464)
                     /locus_tag="ACFI8B_001136"
     CDS             complement(14818..16464)
                     /locus_tag="ACFI8B_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC3834371.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSGYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(16487..17965)
                     /locus_tag="ACFI8B_001137"
     CDS             complement(16487..17965)
                     /locus_tag="ACFI8B_001137"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC3834372.1"
                     /translation="MFSKLSPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVANGAAFVFWDDDGRFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETAHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(17990..19738)
                     /locus_tag="ACFI8B_001138"
     CDS             complement(17990..19738)
                     /locus_tag="ACFI8B_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC3834373.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILG
                     ISPTKPLTAAAVKTPS"
     gene            complement(19799..20062)
                     /gene="ftsL"
                     /locus_tag="ACFI8B_001139"
     CDS             complement(19799..20062)
                     /gene="ftsL"
                     /locus_tag="ACFI8B_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC3834374.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(20089..21045)
                     /gene="rsmH"
                     /locus_tag="ACFI8B_001140"
     CDS             complement(20089..21045)
                     /gene="rsmH"
                     /locus_tag="ACFI8B_001140"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC3834375.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(21042..21497)
                     /gene="mraZ"
                     /locus_tag="ACFI8B_001141"
     CDS             complement(21042..21497)
                     /gene="mraZ"
                     /locus_tag="ACFI8B_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC3834376.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
BASE COUNT         5070 a         6242 c         5435 g         4953 t
ORIGIN      
        1 cagcccaaat accagaattt caatgcccac aatacggcaa caacccatac cataggcggc
       61 acgtccccag tccggcgaca caaaagtttg accacggcat agctgatgaa gccgaaggcg
      121 atgccgtctg caatcgaata ggtaaacggc atgaagacaa tggtcaggaa cgcgggcgcg
      181 gcttcagtca tatcgtccca atcaatgtcc ctcgcactgc ggagcatctg cgtgccgaca
      241 taaagcagtg cgggcgcggt ggcaaatacc ggaacacttt tcgccaatgg ggagaacatc
      301 agacacgcca gcatcaatac gccgacggta accgccgtca ggccggtccg tccgcctgcc
      361 gatacgcccg ccgcgctttc cacataaggt gtggttgaag aagtacccaa agccgcacct
      421 gccacaatgg cggtagagtc tgcaagcagt gcgcgtttca ggcggggcag cttaccgtcc
      481 accagcagtc cggcacggtg ggatacgccg accagcgttc cggtactgtc aaacaaatcg
      541 accaagaaga agacgaaaat cacgctgacc atgctgacgg taaacagacc tttaaaatcc
      601 atctgcataa aggtcggcgc aatgcccggt acttcgccga ccacgccgtg aaactcgttc
      661 aaacccatca ggctggcaat gacggtaatc gtcagaatgg tgatgatgat tgcgccttga
      721 acgcggaaat accccaatac gaccaccatg acaaaaccga acaatgccaa cagtgcgctg
      781 ggctgatgaa tatcgcccaa gccgaccaag gttgccggat tggcaacgat aatgcctgcg
      841 cctttcaggg aaatcagtgc caaaaacaaa ccgataccgg cggcaatcga cattttcaaa
      901 cccataggca gtgcgttgac cagcatttcc ctgactttaa aaaagctgaa caggatgaaa
      961 atcagaccgg aaatgaacac cgcacccaac gccacctgcc aaggcacgcc cataccctta
     1021 acgacggcaa aggtgaaata ggcattcagc cccatccccg gggcaagcgc aatcggatag
     1081 ttgccgataa aacccatgac aaaacagccg atggcggatg cgatacaggt agcgacgaat
     1141 accgccccca tatccattcc ggtctcgccc aaaatcaggg gattgacgat aacgatgtag
     1201 cacatcgtca aaaaggtcgt caaacccgcc atcaactcgg tacgtaccgt cgtaccgttt
     1261 gccttcaggt taaaaaccct atccagcaat gtttgttttg aaatatccat atctagcagt
     1321 ccgtttcaag taaaaaatct gaacccgcac accgcaaacc gcacaggatt cggcatattg
     1381 aaaaatatac ctcccaaatc gggaaaaccg gttcgggagg caataagttt acggcagacg
     1441 gcacattatt tgtctgaatt gtgttgacga cgcaaaattg caggaatttc aaaatcgtca
     1501 aggacagact ggttatcgaa atccgctgct gtcagattca tggtgcggat accgcgattg
     1561 gtgcggatca taccttcgac attgtggctt tgctcctgtt tggacggggc aaccgcttct
     1621 acttcccttg ccggagtagg atcgaccgct cctttttctt tcagaccggt agcgataatg
     1681 gtaatccgga tggcatcttc gctcatggtc tcgtcttcag cggcaccgaa tttgcattcc
     1741 aaatcgggat gcgcgctttg gttgacgatt ttcatgactt cagacaactc ggacattttc
     1801 aggcaacccg gagcagtcgt aatattgacc agcacaccgc gtgcaccatc caaagttaca
     1861 tcgtccagca gcggactaga aatggcctgg tcggtcgcca tacgcgcacg gtcgatacct
     1921 tgggcataac ccgaacccat catagcgata ccgcggttgc tcatcacggt tttcacgtcg
     1981 gcaaagtcga ggttgatgat ttcgctcggg caagttacca cttcggaaat gcctgcgacc
     2041 gcatcgcgca atacattgtc cgccgcgcgg aaggcttcgc gcatagttac gtcttcaccc
     2101 aatgcggtca tcagtttgtc gttcgggatg ataatcagcg aatcgacgtg ttctttcaac
     2161 tgttccaacc ctgcctgtgc cacatggacg cgtttgcctt cataagaaaa cggacgggta
     2221 actacggcaa cggtcagaat gcccaaagac ttggcaatct cagcaacaac cggcgcggaa
     2281 ccggtaccgg taccgccgcc cataccggtc gtgataaaca gcatattggc accgcgaatg
     2341 gcttcttcaa tggcctcccg atcttcctgt gctgccgcac ggccgatatc gggattcgca
     2401 cccgcgccca aaccgcgtgt cagattcgta cccaactgga ttctcttcgc cgcatggttt
     2461 tttgccaaag actgcgcatc tgtattggca ctgataaact ccacactgcg cacattgttg
     2521 gcaaccatgt tattgattgc attgcaaccg ccgccgccca agccgattac tttaattacc
     2581 gcagggctga ctgccgattc tgccacgtcg taaacaaatt ccattcaaaa actcctgatc
     2641 gccccgttca gaggacggtt taaataaatt attatttatt atataagaag tatcttactg
     2701 ctggcaaaat acttctcacc tgtcaaacgg caatccaact gttcagaggt tgttttcaat
     2761 ccaccgcttc aatcttgcca acaaaccgcc gcccccttct ctctcttgca ctgtaccgtt
     2821 ttccggttgc ggcaagtttc cttccagctt gcatgctgca tgaagcagcc cgatagcggt
     2881 agaaaagcgc ggtgtgcgga cacggtcgga caaaccgccc atttcttggg gcgcaccggt
     2941 gcgtacaggc aaatcgaaga ttttttcggc aaattccaca atcccggtca tcatggacac
     3001 accgccggtc agaacgatac ccgcattcag cacttctttg gggaaacccg atttttgcag
     3061 ttcgcccagc actacgccaa aaatctcctg aatacgtgcg ctgatgattg ctgccaaaac
     3121 cttactggaa acctgacgcg atgtccggtc acccacgccc ggaacttcaa tcatctcgcc
     3181 caagccttcc gtatcgcatg atgccacgcc ataatggatt ttaatgtact cggcggcatc
     3241 gagaggtgtt ctcaacgatt tggacaaatc tttggtaatc agattaccac cggccggaat
     3301 gacggacgta tggcggatgg cgccgttcat ataaacggca atatcggtcg ttccgccacc
     3361 aatgtcgatg acgcatacgc cgaggtcttt ttcatcttca gtcagcaccg cctgcccgct
     3421 tgccaacggt tgaagcatga tctgatcgct tttcaagccg cacagctcga tacatttttg
     3481 gacattctgc actgccgtac ttgcaccggt aatgatgtgc acccgcgtat ccagacgcac
     3541 accgctcata ccgatgggct cccttacacc aagttgggtg tcgataatgt agtcttgaac
     3601 cacggcatcg agaatttttt gatcgggcgg gatattgatt gcctttgccg tttcaatggc
     3661 gcggtcgatg tctgcctgtg ttacttctcc gtctttgatt ttaaccacac cttgcgaatt
     3721 aaggctgcgg atgtggttgc ctgcgatacc tgtggtaacg tgggtaattt tggtatccgc
     3781 catcagctcg gcatcattga ccgcctgcct gatggcttgg acggtggcat cgatattggt
     3841 taccatgccc gcgcgcaagc cccgtgaagg agcctgcccc aaaccgatga tgttgatttt
     3901 gtcgtcatct tgaacttccc cgatcagtgc gaggacttta gacgtaccga tatccagtac
     3961 gctgatgtat cttttctgct gttccattgt tcgtctgccc ttaaaactga ttgaaatttg
     4021 cgtcgcaccg tttcagacgg cacggccgta atatgtccga tacctgttcc cactattctt
     4081 cggattcttt ttcgggtaaa ccgtcgggag catggcggac tgaaaatccg tccttatacc
     4141 tcatatccac ataggataac cgatttttat tcttacgcaa cagatgctgc cacgcttcgg
     4201 taaaaagccg gaggcgtttc atctcgtttt cccgtccgag cctgacggtg atgccgttgt
     4261 ccaaaacgac attccacgcc gaacgtgccg tataggtcat ctctttgatg cccaaaccct
     4321 gttttgccaa aacagtcgaa aattcgtcat aacggcggag catttcggca gacgttcctt
     4381 ccgcgcctct gaataccggc attccgggtc tgtccaagcg ggcttcaaaa acattgcctt
     4441 cgccgtccac caaggcatgg tcgccccaac gtgcaaccgg cttgcgctcg gtcaggacga
     4501 cctcaaccgt atcgggaaaa cggcggcgca ccatgaccga cgcaatccac ggataccggc
     4561 ggtaggcttc ctgtgcgcca ttgatgtccg tcctcaaaat attcccatgg atgtattctt
     4621 tcgccaaact gcccaatgcc ttcttatcgg aataaaccag gttgcccttc agcgacacct
     4681 gcttgacggg cagatgattc gaattgtaaa accaaaccag cccggacgca gcaagcagca
     4741 tcgccatcat gacaagcagc cagcgcgtca gccgttccat cgcttcggca ttatcccaca
     4801 tgtgcggcct tcaaaatttc aatacataaa tcggcaaaac ccacgcccgt aacggcagcg
     4861 gatttcggta ctaaactatg gccggtcata ccgggcaggg tgttgatttc caacagatag
     4921 agtttgccgt cggtatcttt gaggaaatcg acgcgcacgc agccttccgc accgattgcc
     4981 tgtgcgccgc gaaccgccag ttcgcgcatc aggctttctt cggcttcggt caaatcttcc
     5041 gaaggacatt gataaatggt gtcgtctcgg ttgtacttgg cttcgtagtc gtaaaactcg
     5101 gttgcgggga tgatgtgtat gccgggcagc cctttgccgt tcaggacggg gcaggaatat
     5161 tcgccgccgc cgataaaacg ttcggcaatg atttcgccct gaaggtgttt caattcttcg
     5221 taaacgcttt tcagacggcc tttttctttg acttttacca cgccgacgct gctgccttcg
     5281 gccgccggct tcacaaacat cggcagaccc aatttttctt cgacggcatc gaaatcggta
     5341 tcatcgtaca gtacggcgaa ctcgggaacg ggtaatccca atgcctgcca aatcagtttg
     5401 cagcggtatt tgtccatgcc gatggcggag gcggcgacac cgctgccggt atagggaatg
     5461 cccaacagtt ccaatgcacc ctgaacagcc ccgtcttcgc cgtaagtacc gtgaaggatg
     5521 ttgaatgccg tctgaaagcc ccgctccttc agttcggata acggcgtttc cttagggtcg
     5581 aaggcgtatg cgtctatgcc tttgcttttt aaggcgttca aaatggcggt accgctgtcc
     5641 agcgagattt ctcgttcgct ggaaaaacca cccatcaata cggccacttt gccaaaattc
     5701 tgcattgttt ttgttctttc ctaattgctt tatgcttgtt gtcagaggtc gtctgaaacc
     5761 tgattgcggt ttcagacgac ctttatatga tgttccgtct gtcaggcggg tgtatctcaa
     5821 atctgtttcg acaattccaa cagcgcggag ggaacgcggt tgatgcttcc cgcacccata
     5881 ttcaacacaa catcgccatc ctgtaaaaca ttcatcagca tttgcggcag gtcggcgacg
     5941 ttttcgcagt aaatcggctc aagtttgccc aatacgcgga tagcacgcgc caaggcgcgg
     6001 gagtcggcgg cggcaaccgg ctcttcgccg gcggcataaa cttcggtcag taccagcgca
     6061 tcaacggtat tgagtacttt ggtaaagtct tcaaacaaat cgcgcgtgcg ggtatagcgg
     6121 tgcggctgga aggcgagtac caaacgtttt tccggatacg cgccgcgtgc agcggcaagg
     6181 gttgccgcca tttcgacggg gtggtgtccg taatcgtcca ccagcaaagc ggtcccgccg
     6241 tttggcaact tgatgtcgcc gtatttttgg aagcggcggc cgacgccttc aaagccgagc
     6301 aagccttttt ggatcgcttc aaccgatgcg ccgacttcca gcgccacgcc gatggctgcc
     6361 aatgcgttca gcacgttgtg tctgccgggc atattcagca cgacttcaaa cgacccctgc
     6421 tcatgtcctt tcatttgaac atggacggtg aatttcattt gcgcgccgac gttttcgatg
     6481 tcggtggcgt agatgtcggc ggtatcgtcc aaaccgtaag tagcataagg tttgctcact
     6541 ttgggcaaaa tcgcgcggac gtgttcgctg tcaacacaca aaaaggcttt gccgtagaag
     6601 ggcatacggt ggatgaaatc gataaacgcc tgatgcagtt tttcgacgct gtgcccgtag
     6661 gtatccatat ggtcttcgtc gatattggta acgacggaca taatcggggt cagatgtagg
     6721 aaagaggcat cggattcgtc ggcttcggca acgatgtatt cgcctttgcc caagcgggcg
     6781 ttggtgcctg cggcgttgag tttgccgccg ataacgaaag tggggtcgag tcctgccgcg
     6841 ccgaggatgg aggcggtcag gctggtggtc gtggttttgc cgtgcgtacc ggcaatggcg
     6901 atgccgtcac ggaagcgcat cagctctgcc agcatcaagg cgcgcggaat aacgggaatt
     6961 tgccgctcca acgcagcgac aacttcggga ttttctttct tgacggcggt agaggcaacg
     7021 acgacatccg caccgttaac gtgttctgcg gtatggccgg gataaacttg aatgcccagg
     7081 ctgctcaaat gctcggtagc ggcatttcgc gcctgatccg aaccggaaac tttaaagccc
     7141 aaattgtgca agacttcggc gataccgctc atgccgacgc cgccgatacc gacaaaatgg
     7201 atgttgctta ctcgattttt catcataatg ttgcgttccg gttggttttt agtgcgtaaa
     7261 ggcgttattt taaaggtctg accgtttgcg caccatagtt ttctgacaaa tatatagcgg
     7321 attgaaataa aaaacatcca tgccgtctga acggcttttc agacggcatg gttcggcagt
     7381 ttacgccgca cacgcaatcg cggcttcggc aacgtcatcc gcgctgtgcg gcaatgccaa
     7441 cgtacgggcg ttttccgccc atttgaggca tttttcgcgg ttgaggctgc cgaggatttc
     7501 ggcgagtttt tccgccgtca actgggtttg cggcaacagc agccccgctt ctgcctgcac
     7561 catgaaacgc gcgttggcgg tttgatggtc atcaacggcg tgaggatacg gcactaacaa
     7621 cgcgcccagc cccgccgccg tcaactcggc aatcgtcagc gcgccggcac ggcaaatcac
     7681 caaatcggca tcacggtagg cggacaccat gtcggtaata aattccacgc attccgcttt
     7741 cacgcccaac gcgtcataat ccgcctgaag attgcccagc ttgttacgcc ccgactggtg
     7801 gtacatctgc gggcgcacct cttcaggcag cagtgccaac gcctgcggta cggttttgtt
     7861 caaaacgtcc gcacccaaac tgccgccgac caccaaaatt ttcaggcggc cttcgcgccc
     7921 ttggaagcgt tcggcaggca cgggcaggtt gctaatatcg gcgcggacgg ggttgccgac
     7981 caaaccgcct tcgtggctga acgctttcgg aaaagcgtac aacacccgtt tcgcccagcg
     8041 cgacaggtgg cggttggaca agcctgccac ggcgttttgc tcgtgaatca caatcggcac
     8101 gcccaagagt ttcgccgcca gaccgccggg aaaggtaaca aaaccgccga agccgatgac
     8161 gcactcgaca cggtgtttgc ggataatccg ctgcgcttcg cggacggttt tgtacagagt
     8221 aaacggaagc atcagcttgc gtttgatgcc gttgccgcgt attcctttaa tcgccagcgt
     8281 ttccaagcgt atgccgtatt gcggcacgat gcgctcttcc atcgaatcct tgctgcccag
     8341 ccaaattaca tgatgaccgc gcacgcgcaa tgaatccgcc acagccagag ctgggaaaat
     8401 gtgtccgccc gttccgcccg ccatcagcat aaaggtttta ccgcccatga tttactccac
     8461 ccggtaaccg cgcattttct ggcggttttc ataatcgata cgcaacagca gcatcatgct
     8521 gatcagcatg aaaaagactg acgaaccgcc ataggacatc aacggcagcg tcagaccttt
     8581 ggtcggcaaa gcaccgatgt tcacaccgat attgaagaaa ctttggatac cgatccaaat
     8641 gccgataccc gaagcgatat aggcgttgaa agtcaaaccc aaatcgcgcg actgcttgcc
     8701 gatggaaaac gcccgcacca ccagccagcc gtaacagaat atcagcacgc acatcccgaa
     8761 gaagccgaat tcttcagcga tgatggcaaa aataaaatcg gtatgcgctt ccggcagaaa
     8821 gccgcgtttg ctcaaactcg cacccaaacc cataccgaac cactctccgc gcccgattgc
     8881 catcagagag tgggtaagct ggtagccggc accctgcggg tctttccacg ggtccaaaaa
     8941 tgccactacc cgctgcacac ggtagggagc ggcggtaatc atcagcacca tcccacccaa
     9001 gacgctgcct accaggacaa aaaaatattt ccacggcaat cctgccagaa acagcattcc
     9061 aacggtaatg acggtaatga cgacaaacga accgaaatcc ggctgtacca ttatcagcac
     9121 caaaccgaac gccaccagca taatcggcag gatgatcgcc cggaaacggc cgtacatttc
     9181 taatgtttca cgacgtgcct gcggattggt ggcggacata atcaggttgg ccgtcccccg
     9241 ccaaatcgac tgccaaccca aactttccat gctgcgcaac acttcttcac ggcgcgtgaa
     9301 caggcttgcc aaataaagga tgactgccag cttgaacagc tcggtcggct ggaaattcaa
     9361 cggacccaaa ggtatccaac gggtcgcgcc attgatttcg cgcccggcaa tcaatacggc
     9421 taccagcaac aggccggata aggcaaaaat ccacggcaca agccgccgcc atgtcctcat
     9481 cctgcaaaga aaccataaaa aaccgctcgc tataaggccg gcaacgacga accccgcctg
     9541 cctggtcaaa tagaaaaact gatcgccgcc ttccttcgat gccaaataca cagaagccga
     9601 ataaatcatc agcaggccga acgccgtcat cagcaccacc atccacaaaa gcggcgcgtc
     9661 gaatttcctg ccgtcgcgca caatcggcct gtcgagcagc agagtgtgga caccgtcgcc
     9721 cacttttacc aatacttccg aaatcttcaa aaaaccacct gccagtctgt ttgcacctgc
     9781 cgcaaagggc aaaaatttca gacggcagac aatgccgtcc gaacatacga tacatcccaa
     9841 atcggtattc taaatcttta cttgccgccc aacaatgacg gcgtttgcgt ttcggacggc
     9901 atcacaaagc cttaaacgct tcgataaaca cttccgaacg gtgcgcgtag cctttaaaca
     9961 tatcgaaact cgcgcaggcg gggctgagca agacaatatc gcccgcttcg gcttgggcgt
    10021 atgccgtctg aaccgcctct tccaaagtga cgcagtcggt caggttcaag ccgcagccgt
    10081 ccaaatcgcg gcggatttgc ggcgcatcga cgccgatcag gaacacgcct tttgccttat
    10141 ctttcaacgc gtcgcgcagg ggcgtgaagt cctgcccttt gcccatgccg cccaaaatca
    10201 cgaagagcgg gttttgcaaa ccggcaatcg cggcggcggt cgcgccgaca ttcgtgcctt
    10261 tgctgtcgtc gatgaatacc acgccgtttt tctcgccgat tttttccaca cggtgcggca
    10321 agccttggaa ggttttgacg tgttccagca atgcttcgcg cggcaaaccg acggcctcgc
    10381 acaaagccac ggcagccata acgttggcgg cgttgtgcaa accttgcagc gggatgtctt
    10441 gcgtagaaat caaatcttca ttgccttgtt ttaaacagcc cgtcccgcgt tccaaccaaa
    10501 aatcggcttc gtgttccaag gaaaaccgtt tcacttcacg ccctgcccgt ttcatggcgc
    10561 ggcagaacac gtcgtccgca ttcaaaacct gcactccatc gccacggaaa atcttggctt
    10621 tggtatgcgc gtagtcgagc aaatcgtcgt agcggtcgag atggtcttcg gaaatgttca
    10681 tcaccgtcgc cgcagtcggg cgcaggcttt cggtgttttc cagttggaag ctggaaagct
    10741 ccaacaccca cacgtccgcc tttttgcctt cgcgctgcaa ttctgcctcc aaaaccggcg
    10801 taccgatatt gcccgcaatg acggtatcca gcccgcactt gatgcagaga tagccgacca
    10861 ggctcgttac cgtggttttg ccgttgctgc cggtaatcgc aattaccttg tcgccgcggc
    10921 ggttcacaat gtccgccagc aattcgatgt cgcctagcac gcgcccgccg ttttgcttga
    10981 acgcctcgat atccggctgc cgctcgctga tgccgggact gagcgccaga atatcgaaac
    11041 cgttgtccaa cgcatctttc agacgacccg tgtaaaacac cagcccgtca aacatcttac
    11101 cgatttgcgc cacgcgctcg gctttcagct ccgcatcata agcggcaacc tccgcgccgt
    11161 ttttgcgcag ataggcaatc atggaaatac ccgtgccgcc aagtccggcg acgaggattt
    11221 ttttgttttg gaaagtcatt ttggttttgt cctaaaacaa atcatattgg gcaggagacg
    11281 tccgcccttg cccaagccgc tttcagacgg catcgcgagc cgattaataa cccgccttca
    11341 ggcgttggtc attgtcgcag ctgttttggt ctccgttttg acaagccttg ccaagccatt
    11401 gttgagcgag cgcaaggtct tggcgcacgc cgcgtccatc gtaatacatc aagcccaaat
    11461 tgtattgggc ttgggcatcc ccttgttctg aagccttgcg ataccactgc actgcctgta
    11521 cataatcttg acgaactcct tgtccatttt catacatcac gcccaaattg aattgggctg
    11581 ctgcatttcc ctgttctgcc gcctgcaagt tttcccgaaa atccgatacg tcacccgccc
    11641 acaccgcttg gttcaagccc aaggcaatca gggcggcggc aagccatttg actgtctgtt
    11701 tcatggtttt ctcctgtttt agtataaggc gggtttcagc cgccgttaac gataggacgg
    11761 ggcggattgc cgccgcaggt ttattgcgcg ttcaaatgcc gtctgaaagg tgttcggacg
    11821 gcataggttc agcgaatttt gagggtactc aaaccgatca ataccaagac gatggtaata
    11881 atccagaaac ggacgacgac ttgggtttct ttccagcctt tttgttcgta atggtgatga
    11941 atcggcgcca tcaggaagat gcgttttttg gttttcttgt accagccgac ctgaagcata
    12001 acggacacgg cttctacgac gaacagaccg cccataatga cgaggacaaa ttcttggcgg
    12061 acgatgacgg caacggtacc gagcgcggca cccaatgcca gcgcgccgac atcgcccata
    12121 aagacttgcg cgggataggc gttgaaccac aaaaatccga ggcacgcgcc gcacatggcg
    12181 gtgcagaata tagcgacttc gttcgcgccg gcgacatagg gaagctggag gtattgggaa
    12241 aattggtagt gtccgctgac gtaggcgaaa atggcgagcc cggcggcaac gaggacgacg
    12301 gggaaggcgg caaggccgtc caagccgtcg gtgaggttga cggcgttgga tgtgccgacg
    12361 atggtcaggt aagacaacac caaaaagccg accacgccca gcggcagggc gatttgtttg
    12421 aaaaacggga caatcaaaat attgttggcg gaattggcgg caaggtaaaa caatgccaaa
    12481 ccggcgataa cggcaacgct tgactgccac accattttga atttggcgga cacgccgttg
    12541 gggtctttat aaacgacttt gcgccagtcg tcgtaaaaac cgagcgcacc ggtggcaagc
    12601 agtacgccca agagaatcca gatatacggg ttcgcccagt tgccccacaa cagggtggac
    12661 acggtaatgg cggtcagaat cagcgaaccg cccatcgtcg gcgtgccgtt tttgacgagg
    12721 tgggtttgcg ggccgtcggt acgcactgcc tgcccgcatt tgagcgcggt cagcctgcgt
    12781 atcgtccacg ggccgaacat cagggaaaac gccaaggcgg tcaacgccgc cataacggcg
    12841 cggaatgtgg tgtattgaaa aatattcaga ccggttaacc agttgctgaa atgtgcgagc
    12901 cataaaaaca tggggcttcc ttttttgttt tgtcgttata gcggattaac aaaaaccggt
    12961 acgtcgttgc cccgccccgg ctcaaaggga acggttccct aaggcgccca agcaccgggc
    13021 ggatcggttc cgtactatcc gtactgtctg cggcccgccg ccttgtcctg atttttgtta
    13081 atccgctata catcgggctt gcgcccgctt gaggtttgct tgctgtccgg caaggtttcg
    13141 gacggctttt gcagattaac gtgcagggac tttcccaatc acgcaaatcg ggtagctccc
    13201 cggatttttg ccgccgccca agtctaaaca taaatcttca taaagatacg cctgcgtccg
    13261 cattccggca taaaacgcgc cgaccagcgc ggcaatcagc aataaggctt taaaaaaacg
    13321 tcggcttttc attttcactt atcctccaat gcctcgacca cttcttccat ctgcataaag
    13381 cgcgaacctt tcaccaacac ggtggcgcgt tcgggcaaat cgtggctcaa cacttgaatc
    13441 aacgggtctt tggcggcgaa ccacaaaccg tccgcgccaa atttttccgc cgcttcgacg
    13501 ctgttgtcgc cgacaaaata agccgcttcg atgccttggt cgcgggcgta cgcgccgact
    13561 tcggcgtgca tggcggcggc ttcgtcctcg cccagttcgc ccatatcgcc catcacgaaa
    13621 atgcgcggcg caggcatacg cgccaacacg tcaaccgcgg ctttcatact gtcgggattc
    13681 gcattataag tatcgtcaat cagggttgcg cccttgatgc cggctttgac gttcagacgg
    13741 cctttgatgt tgctgaagcc ttgcaaacct tccgccacat cgttcaaact caaaccggca
    13801 gccaaagcca gcgcggcggc agcggcggcg ttgtggacat tgtggcggcc gggaacaggc
    13861 agcaccacgg cagtgcgctc gtcgccgcac accaaatcaa attcgcacga caaaggtttc
    13921 agcacgatat tttccgcgcg gacatcgccg ctatcgacgc cgaaagtgca cgtattcaaa
    13981 ttaaacgttg ccgttttgaa gacagccata ttggcatctt cttgaggaat cagtgccatg
    14041 ccgtctgaac ataagcctgc ataaatctcg cttttcgctt tggcaatatc gcccactccg
    14101 tcgaaaccgc atccgacatg ggcgcgcagg gcgttgttga ccaaagcggc atcgggtttg
    14161 gcgatttgcg ttaaaaccgc cagttcgcca aaatggttca tgcccatttc aatcacggca
    14221 tagcggtgtt tttcgtttaa tttcaataaa gtcagcggca atccgatgtg gttgttgaag
    14281 ttgcctgccg tcgccgaaac ggcatcatcg ccgaaacggc ggcgcaatac cgcagccagc
    14341 atctccttca ccgtcgtctt gccgcccgaa ccggtaatgc cgaacacaaa cgggttcaca
    14401 ttatcgcgcc acgccttcgc caacgtttgc aacgcggcaa gcgtgtcatc gactttcaac
    14461 gcgccgccca aagccgcgca atcttcgcgc gaaaccacaa ccgccgccgc gcccgcagac
    14521 aatacgcctc caacaaaatc atgcgcgtca aaccgcccgc ccgccaatgc gaaaaacaca
    14581 tcgccttccc gaatatcgcg gctgtcggtt acgatgcgcg acacgggttt gttttcagac
    14641 ggcatcggaa gcttgagggc ttggcagatg aaatttaggt ccagtggttt catatttgct
    14701 ttcgttaata ttcgggcggc ggacacatcg gtagcggctg atttttttat cgcctgtttt
    14761 gctgtggtaa aacacagatt attttcccat tctcattcgg cattttttct gtacgtatca
    14821 ttttttagac gtatttttag ccgatttgcc ttttcccgca taccacggcg cggggtcgtc
    14881 ggactgtctg tcgataaagg caaggttatt gccttcgccc ggcacatcgg ggacattccc
    14941 ccaaaaatca tagccgtcat cgggcaactc gtcggtttcg atacccgtcc aactgccgaa
    15001 tccgcgtaaa aaattaaacg cgctgcgcct cactttaatc atttcgcgcc gcgtgtcatc
    15061 ggacgaaatt ttaaccagcg gcacgccgta gctttgacgc ttccactcgc cgtgtgtcaa
    15121 cgttcgctcg ccgcctttgc cgacatgcat caaaccgtgg tcggaaaagt aaaccaaaga
    15181 ccagctttct ttattttcat tcaatatctt aaccgtatct tctaaaaatt tatcggtttg
    15241 cgcgatggtg gaaacatagc aggatatttt ttcagtttga tactgaaacc gccgcgcatc
    15301 cttatccaaa cgtgtgcaaa aatcactgtg cgaacccatc aggtgcatca caatcagccg
    15361 aggcttcgtg cctgcatttc ctgtcaaaac ccgtttgaac gccggcaaca aaaggcggtc
    15421 gctcaacccc gcgcttttgc cataatcacc cctttgggta aaccacggat aatcgctgcg
    15481 tagggcatag gtggaaattt cgttggcaaa atgccccaac attccttgat tagacagcca
    15541 cgccgtccga aaacccgcct gcttcgccaa gctgacaatg ttattgttcg gctctcccgg
    15601 cagccccaaa gtctgcggca gcgaaagatt cgtcgcgtgg gcggtcgatt ggtaaccgtt
    15661 tatcaacagc cctttggtcc gactcaaaaa aggcgtatcg ggcaatggga aaccgtaaac
    15721 attcatataa cccgaacgcg cgctctcacc gataaccaca acataatttt tatacttggg
    15781 cgcaacatga cggatatgcc atgttgacgc tttttttgct gcttccaaaa tgtgggcgcg
    15841 cttggcggca tattgtgccg gcgcggaagc caaatcgtaa tacaggtcga aaatattcaa
    15901 caacaggccg gcatcaggtt ctcgccaatc tttatcgccg gcgattttct ccatcaccgc
    15961 gcaggataaa atcaaagtca ataatatggt cagccatatt ttgctgcggc gtttatagtt
    16021 ttttacgtca gcaaataccc ccacagatac acaatatttc caaactgtca agccaaaaat
    16081 gaaaaataat gcctgcacaa aataaagcga cccgggaaga ttgccgacaa attcacgcgc
    16141 ctcggcagga ttgctttcca atatcgaacc gactatctga taagaaggcg caccatacag
    16201 ccagccgacc ggcaaatata gggcggttgt gccgacgtaa atcagtaaaa caactgaaga
    16261 aacgcgcggg aagccgcgtg ccaataataa taaaaataca accgaagcca aagcgcccac
    16321 ggcagaataa cggtagccgt attcatattc caagtgataa cctgatgcga tggccgcgcc
    16381 aaaacaaaag gctaaaagcg cgcaaatgcc caatgtattt tttaatgtct gatccatatt
    16441 ttttattcgg gcggattgtt tcatacgatg ccgtccgaat atcccattat ttacgagtta
    16501 acaaagcctg cccgacgatt tcaagatcgg aaaaacggtg cttcacgccc tgtacatcct
    16561 gatagttttc gtgtcctttg ccggcaatca ggatgatgtc gtttgcggcg gcctgctcaa
    16621 ccgcataacg gatggcggcg gcgcggtcgg cttcgacgca ttcgggcgcg ggcacggcgg
    16681 gcaggatgtc gttgatgatg tcgtgcggat tttccaaacg cgggttgtcg ctggttacga
    16741 cgactttatc cgcgccctgt acggctgccg cgcccatcaa tgggcgtttg ccgcaatcgc
    16801 ggttgccgcc gcaaccgaat acgcaccata aggccgcacc ctgcggtttg atttcctgca
    16861 aagtggagag tgctttttcc aatgcgtcgg gcgtgtgggc gtaatcgaca acgaccaagg
    16921 gcttgccgct gttcatgatg cagtccatgc gccctgaagc gggacggatt tttgccagca
    16981 catccaatac cttatcaagc ggataaccgt tggcacaaag caaggcgatg caggcggcga
    17041 ggttttgcgc gttgaaccgt ccgagcaggc gggtgcggca ttttccttcg ccccacgggg
    17101 tttggaatac tgcttccatg ccgtctgaag aggcggtaaa gtcggtaatg cggatgtcgg
    17161 cgtgttcgct gaaaccatag ctgtaaacgg ctaaatcggg acagtctttt ttcagacgac
    17221 ctgcgagttc cgcgccgtat ccgtcatcgg tattgataac ggcgtgtttc aagccgtgcc
    17281 agtaaaacag gcgcgacttg atagcaccgt aggcttccat cgtgccgtgg tagtcgaggt
    17341 ggtctcgggt gaggttggtg aagattgcgc tgcggaacgg cacgccgttg actcgcgact
    17401 ggtcaaggcc gtggctggaa acttccatcg cggcggctgt tgcgccttgt tgacggaaac
    17461 ggtagagcag ggtttggaca tcgacggggg cgggggtggt gtgcgcggtt tcttccaatg
    17521 caccccaaaa gccgttgccg accgtgccga taatggcggt tttttcgccc aacaaatcgg
    17581 cggcttgcgc cagccattgc gtgatggagg ttttgccgtt ggttccggtt acgccccaga
    17641 ctttgagacc gtctgaaacg ttgccgtaaa cctgtgccgc caatatgccg gcacggtgtt
    17701 tcaaatcttt gatgccttga ttggggactt tccattcggg attccacgcg aatctgccgt
    17761 cgtcgtccca aaaaacaaaa gccgcgccgt tggcaacggc ggcggggata taactgcggc
    17821 cgtcggtata ttcgccctga caggcaacga aaatatcgcc ttgtttgatt tggcggctgt
    17881 ctgaatgcaa caaacgccct gccgcgtttg cacacgacag ggtcgggatg ccggtttcag
    17941 ccaaagggct taacttgctg aacataaaac aatctcgttg atactcggat taagacggtg
    18001 ttttgacggc tgcggcggtc agtggcttgg tcggggaaat gcccaagatg ttcaggctgc
    18061 cgcccataat ttttttgaag ggcggccctg ccactacgcc gccgtaatag ccgtgggcag
    18121 tcggttcgtc gatggttacc gccacaatca cacgggggtt tttggcgggg gcaaaaccga
    18181 taaacgtacc gacgtgtttg ttgtccacat agcggccatt gaccagtttg cgcgccgtac
    18241 cggttttagc gccgacatcg aaaccgtcca ccgcacccgc cgtaccggtg ccgcccggct
    18301 cggttacgga aaccatcaga ttgcgtacct cgcgcgcggt cgattctttg aatatgcgtt
    18361 tgccttgcgg cgcaaccgcc tgcttctcaa agctgagcgg cagcaaaacg ccgtcgtgcg
    18421 tcagtgcggt ataggcgcgc gccaattgca gcaggctcaa ttgcaggccg taaccgaaag
    18481 acatcgtcgc ctgttcgatg ggccgccacc tgcgccaatt tctcaacaaa cctgcagttt
    18541 cccccggaaa gcccgagtgc atacgcacac cgatgcccaa ttcatgatag aagtcataca
    18601 tttcttcggc gccgaaacgc gcagacagtt tgcttgtgcc gacgttggac gatttctgca
    18661 taatgccgcg cacatccaaa gaggggtaaa catgggtatc gtcgcgcacg ggagacggtc
    18721 cgattttata aggctgcgta ttcagccgtt cgttcaaatc ggttttgccc gcatccaatg
    18781 ccttcgcaat cacgaacggt ttgattgccg aaccaggttc gatcatatcg gttacggcac
    18841 ggttgcgccg ctgttcgctg tctgcccggc cgggtctgtt gggatcgtag gcgggcgtat
    18901 tggccaaggc gaggatttcc cccgtgcggg catccaaaac caccaccgtt ccggcttttg
    18961 cctgatggta ttcgaccgcc ttgttcaact cttcataggc caaggtctga atcctctgat
    19021 cgagggaaag gatgatgtct ttgccgtttt gcggtgcttt attgcgcggg gagtccaagc
    19081 tgtccacaat attgccctgc cggtcccgca aaacaacttc cgcgccgtct tcgccataca
    19141 ggctgtcttc aagcgaaagt tccaaacctt cctgaccttt gccgtcaata tcggtaaatc
    19201 cgatgacgtg tgcaaacagg ttgcccatcg ggtaatggcg ttttaattct ttttcaaata
    19261 caaagttttc caaacccaag gctttgacct cttcggcaac cttgggatcg agctgccgct
    19321 tgatccaaat aaacgacttg cctttctgtt cgagtttgtt cctcaaaaca tcgaccggca
    19381 catcgacaag ctcggacagg cgttccaatt gggcggcaga cggcatttcc ttcatatctt
    19441 taggcacggc aaacagggac tccgtcggcg cgctcaacgc caaaaccgca ccgttccggt
    19501 ccgaaaccgt accgcgtgta gccggcaatg cttgagtccg cacaatccgg ttgtcgccct
    19561 gttctttcaa aaagttatac gttaccgtct gcagatacag cccgcgggca atcagacagg
    19621 caaacaagac cgccattgcc atcaggacga agctaatccg tccgttactg gtcatcggct
    19681 ttttgacctg ctcttctttg ggcagcatcc ggggcttata ttcgctttta atcaacattt
    19741 ttacttctcg ttattattat cctgatgcag gaatccgatt ccggcacaca ggctgcttct
    19801 atctttgatg ttccaccata aaggtattgc ccgaaaccgg cggatggagg ttttgttttt
    19861 ctgccgccgc cctgatcgct tcgtgattcg ccaaacgcgc ctgttgcacc ctcatttgcg
    19921 cataatcctg ctccaaggcg atttcctgtt ttttcgcctt gtccaaagct gtgaagttga
    19981 gcctgtactg gttttgctgc atcacaacgg aaaaagcgga aacgcacacc gcaagcagca
    20041 gaaagaaatt cgatttgttc attgccgttc agacgttttt ctctgttatt attccggtat
    20101 cggaccggca gtccgctccg ccacacgcaa aactgcgctc ctcgccctcg ggttggcggc
    20161 aatttccgct tcacccggct ttaatgccct gcccacgatt ttcaggggcg gctcgggcaa
    20221 atccgcttct ctgaccgccg cccagctcgg caggggctcg tgttgcgaat attttttgac
    20281 aaactgcttc acaatgcggt cttccaacga atggaaagca atgaccgcca aacgcccgcc
    20341 ctctttcaga cggcacatga cctgcggcaa taccgcccct acttcttcaa gctcgcggtt
    20401 aataaagatg cggattgcct ggaaggtgcg cgtcgcagga tcctgccccc gctcgcgagt
    20461 acggacgttt tgtgccacga tctgcgccag cttgcgggtt gtatcgattg gactttccgc
    20521 ccgttgcgcg acaatggcgc gcacaatctg gcggctaaac cgctcttcac cataattctt
    20581 gattacctcg tgcaaatcct gttccgacgc aaccgctatc cactctgcgg cagacatacc
    20641 gcgcgtcgta tccatacgca tatccaaagg ggcatcgaaa cggaagctga aaccgcggct
    20701 gccgtcatcg atttgcggag acgaaatccc caaatcaaac agcgcaccgt ccaccttgcc
    20761 gatacccaaa ccgtccaatg ccgtctgaaa cgaagcaaaa ccgccatgca cgacaccgac
    20821 ccgtttgtcc gaacgcgcca gctcttctgc cacagcaatc gcctgcgggt ctttgtcgaa
    20881 aacaatcaac cgccccgcat cgcccaaacg cgacaaaatc agccgggaat gccctcccct
    20941 gccgaacgta ccgtccacat agacaccgtc ttcgcgcacg gcaagcgcat ccaccgcctc
    21001 attcagcaag accgtgatat gccggtaact ttctgctcca ctcacaattg caaatccgtc
    21061 tgactcaact ggaaggcaag ttcgtcagga tcgtcatcca aagcctgaac catctcagcc
    21121 tcccactgct cgcgacccca aagctccaaa cggttggcac ggccgaccaa aacaacttca
    21181 cggtcgaaat ccaccctttt cctcagtccg gcaggaacca gcaccctgcc ggcgctgtcc
    21241 cattccaaaa tttccgcgtt atgcagcaaa agattttgaa accgccgcaa aacagggtta
    21301 tccgccactt ttaagtttaa aagttgcgcc gcaacctttt cccactccgc aacagggtac
    21361 atcaacagct tatgtttcga ctcgagcgtt gccactacgg caggcgtata gaggcgcgac
    21421 agaatgtcgc ggaatttggc aggaacagcc aaccgcccct tactgtcgat gcttaattcg
    21481 tgtgcgccgc cgaacatgac atgtcccaag ccgaaatcaa aatcacaagg gtaaaagaga
    21541 cactttgccc cacaattccc caccaatcga cactataaga aattttaaac actcggtcaa
    21601 atcagggcat gaaaactcat taacatatct gaaattttta ttctgtttaa aaacaataag
    21661 ataaaaaatg acgacaacgg cacggcgggt gcagtacaga
//
LOCUS       DAXFWM010000032        21592 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000032 DAXFWM010000000
VERSION     DAXFWM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21592
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.032"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1602
                     /locus_tag="ACFI8B_001142"
     CDS             <1..1602
                     /locus_tag="ACFI8B_001142"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC3834377.1"
                     /translation="AFAWLWPGVFSHDLWNPAEPAVYTAVEALAGSPTPLVAHLFGQT
                     DFGIPPVYLWVAAAFKHLLSPWAADPYDAARFAGVFFAVIGLTSCGFAGFNFLGRHHG
                     RSVVLIHIGCIGLIPVAHFLNPAAAAFAAAGLVLHGYSLARRRVIAASFLLGTGWTLM
                     SLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLTAVASLAFALPLMTVYPLLLAKTQPA
                     LFAQWLNYHVFGTFGGVRHIQTAFSLFYYLKNLLWFALPALPLAVWTVCRTRLFSTDW
                     GILGIVWMLAVLVLLAFNPQRFQDNLVWLLPPLALFGAAQLDSLRRGAAAFVNWFGIM
                     AFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSPYYVPDIDPIPMAVAVLFTPLWLWAI
                     TRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWLDAAKSHAPVVRSMEASFSPELKREL
                     SDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDVRCRYRIVRLPQNADAPQGWQTVWQG
                     ARPRNKDSKFALIRETGENILKTTD"
     gene            1809..2414
                     /locus_tag="ACFI8B_001143"
     CDS             1809..2414
                     /locus_tag="ACFI8B_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC3834378.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            2486..2878
                     /locus_tag="ACFI8B_001144"
     CDS             2486..2878
                     /locus_tag="ACFI8B_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC3834379.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            2883..3029
                     /locus_tag="ACFI8B_001145"
     CDS             2883..3029
                     /locus_tag="ACFI8B_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834380.1"
                     /translation="MPSERNRVSDGILSDGAQTESVCRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(3077..3523)
                     /locus_tag="ACFI8B_001146"
     CDS             complement(3077..3523)
                     /locus_tag="ACFI8B_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC3834381.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERIEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(3655..3867)
                     /gene="rpsU"
                     /locus_tag="ACFI8B_001147"
     CDS             complement(3655..3867)
                     /gene="rpsU"
                     /locus_tag="ACFI8B_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC3834382.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4157..6007)
                     /gene="ltgA"
                     /locus_tag="ACFI8B_001148"
     CDS             complement(4157..6007)
                     /gene="ltgA"
                     /locus_tag="ACFI8B_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC3834383.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARATLRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQHSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            6384..7121
                     /locus_tag="ACFI8B_001149"
     CDS             6384..7121
                     /locus_tag="ACFI8B_001149"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC3834384.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            7123..7809
                     /locus_tag="ACFI8B_001150"
     CDS             7123..7809
                     /locus_tag="ACFI8B_001150"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC3834385.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            7968..8834
                     /locus_tag="ACFI8B_001151"
     CDS             7968..8834
                     /locus_tag="ACFI8B_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC3834386.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(8897..9466)
                     /locus_tag="ACFI8B_001152"
     CDS             complement(8897..9466)
                     /locus_tag="ACFI8B_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC3834387.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            9624..10484
                     /locus_tag="ACFI8B_001153"
     CDS             9624..10484
                     /locus_tag="ACFI8B_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC3834388.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            10620..10796
                     /locus_tag="ACFI8B_001154"
     CDS             10620..10796
                     /locus_tag="ACFI8B_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834389.1"
                     /translation="MNTRINFTATVGKRVSQCYVTSVISTIGVTTSDAICSGGGTNKA
                     KSQCNALLKAAGRC"
     gene            11159..11512
                     /locus_tag="ACFI8B_001155"
     CDS             11159..11512
                     /locus_tag="ACFI8B_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC3834390.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            11502..12368
                     /gene="atpB"
                     /locus_tag="ACFI8B_001156"
     CDS             11502..12368
                     /gene="atpB"
                     /locus_tag="ACFI8B_001156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC3834391.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSVPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            12425..12661
                     /gene="atpE"
                     /locus_tag="ACFI8B_001157"
     CDS             12425..12661
                     /gene="atpE"
                     /locus_tag="ACFI8B_001157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC3834392.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            12731..13201
                     /locus_tag="ACFI8B_001158"
     CDS             12731..13201
                     /locus_tag="ACFI8B_001158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC3834393.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            13206..13739
                     /locus_tag="ACFI8B_001159"
     CDS             13206..13739
                     /locus_tag="ACFI8B_001159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC3834394.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            13750..15297
                     /gene="atpA"
                     /locus_tag="ACFI8B_001160"
     CDS             13750..15297
                     /gene="atpA"
                     /locus_tag="ACFI8B_001160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC3834395.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRSIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            15322..16197
                     /gene="atpG"
                     /locus_tag="ACFI8B_001161"
     CDS             15322..16197
                     /gene="atpG"
                     /locus_tag="ACFI8B_001161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC3834396.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            16235..17632
                     /gene="atpD"
                     /locus_tag="ACFI8B_001162"
     CDS             16235..17632
                     /gene="atpD"
                     /locus_tag="ACFI8B_001162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC3834397.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            17643..18065
                     /locus_tag="ACFI8B_001163"
     CDS             17643..18065
                     /locus_tag="ACFI8B_001163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC3834398.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            18237..19133
                     /gene="glyQ"
                     /locus_tag="ACFI8B_001164"
     CDS             18237..19133
                     /gene="glyQ"
                     /locus_tag="ACFI8B_001164"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC3834399.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            19250..21313
                     /gene="glyS"
                     /locus_tag="ACFI8B_001165"
     CDS             19250..21313
                     /gene="glyS"
                     /locus_tag="ACFI8B_001165"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC3834400.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEMIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            21377..>21592
                     /locus_tag="ACFI8B_001166"
     CDS             21377..>21592
                     /locus_tag="ACFI8B_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3834401.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNSGLI"
BASE COUNT         4987 a         5795 c         5730 g         5080 t
ORIGIN      
        1 gcgtttgcct ggctgtggcc cggcgtgttt tcccacgatt tgtggaatcc tgccgaacct
       61 gccgtctata ccgccgtcga agcactggca ggcagcccca cccccttggt tgcccatctg
      121 ttcggtcaaa ccgatttcgg cataccgccc gtgtatcttt gggttgccgc cgcattcaaa
      181 catttgctgt cgccgtgggc agccgacccg tatgatgccg cacgctttgc aggcgtattt
      241 tttgccgtta tcggactgac ttcttgcggc tttgccggtt tcaacttttt gggcagacac
      301 cacgggcgca gcgttgtttt aatccatatc ggctgtatcg ggctgattcc ggttgcccat
      361 ttcctcaatc ccgccgccgc cgcctttgcc gccgccggac tggtgctgca cggctactcg
      421 ctggcacgcc ggcgcgtgat tgccgcctct ttcctgctcg gtacgggttg gacgttgatg
      481 tcgctggcgg cagcttatcc ggcggcgttt gcgctgatgc tgcccctgcc cgtgctgatg
      541 tttttccgtc cgtggcaaag caggcgtttg atgttgacgg cagtcgcctc gcttgccttt
      601 gccctgccgc ttatgaccgt ttacccgctg ctcttggcaa aaacgcagcc cgcgctgttt
      661 gcgcaatggc tcaactatca cgttttcggt acgttcggcg gcgtgcggca cattcagacg
      721 gcattcagtt tgttttacta tctgaaaaat ctgctttggt tcgcactgcc cgcgctgccg
      781 ctggcggttt ggacggtttg ccgcacacgc ctgttttcga ccgactgggg gattttgggc
      841 attgtctgga tgcttgccgt tttggtgctg ctcgccttta atccgcagcg ttttcaagac
      901 aacctcgtct ggctgctgcc gccgcttgcc ctgttcggcg cggcgcaact ggacagcctg
      961 aggcgcggcg cggcggcttt tgtcaactgg ttcggcatta tggcgttcgg gctgtttgcc
     1021 gtgttcctgt ggacgggctt tttcgccatg aattacggct ggcccgccaa gcttgccgaa
     1081 cgcgccgcct acttcagccc gtattacgtt cccgacatcg atcccattcc gatggcggtt
     1141 gccgtactgt tcacaccctt gtggctgtgg gcgattaccc ggaaaaacat acgcggcagg
     1201 caggcggtta ccaactgggc ggcaggcgtt accctgacct gggctttgct gatgacgctg
     1261 ttcctgccgt ggctggacgc ggcgaaaagc cacgcgcccg tcgtccggag tatggaggca
     1321 tcgttttccc cggaattaaa acgggagctt tcagacggca tcgagtgtat cggcataggc
     1381 ggcggcgacc tgcacacgcg gattgtttgg acgcagtacg gcacattgcc gcaccgcgtc
     1441 ggcgatgtcc gttgccgcta ccgtatcgtc cgcctgcccc aaaacgcgga tgcgccgcaa
     1501 ggctggcaga cggtctggca gggtgcgcgc ccgcgcaaca aagacagtaa gtttgcactg
     1561 atacgggaaa ccggggaaaa tatattaaaa acaacagatt gagccgaatt tctggattaa
     1621 gtgccggaaa tcgcgtataa ttgcgcgatt aaacctttat atagtggatt aaatttaaat
     1681 caggacaagg cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgccg
     1741 taccggttta aatttaatcc actataaatc agccgttttg caggcatcac acaggagcga
     1801 caattattat gatgaccctc tattccggca ttacctgccc cttcagccac cgctgccgct
     1861 tcgttttgta cgaaaaaggt atggattttg aaatcaaaga catcgatatt tacaacaagc
     1921 ccgaagacct cgccgtcatg aacccgtaca accaagtccc cgtgctggtc gagcgcgatt
     1981 tggtgctgca cgagtccaac atcatcaacg aatacatcga cgaacgcttc ccccatccgc
     2041 agcttatgcc cggcgatccc gttatgcgcg gccgggggcg gctggtgctg taccgtatgg
     2101 aaaaagaatt gttcaaccac gtccaagttt tggaaaaccc cgccgccgcc aacaaggaac
     2161 aggcaaaagc gcgcgaagcc atcggcaacg gcctgacgat gctttcccct tcgttcagca
     2221 aaagcaaata catcctcggc gaagattttt ctatgattga tgttgccctg gccccgctgt
     2281 tgtggcggct cgaccattac gatgtcaaac tgggcaaaag tgccgcgccg ctgctcaaat
     2341 acgccgagcg tatcttccag cgcgaagcct ttatcgaagc actgacaccc gccgaaaaag
     2401 ccatgcgcaa ataagtccga aatgcttgca aaacccgccg ttttgcaggc attttcacat
     2461 tcaggcacac cacacggaac ccatcatgcc cacctcaacc aaaccctaca tcctccgcgc
     2521 actttgcgaa tggtgcagcg acaacagcct cacgccgcac atccttgtct gggtaaacga
     2581 gcatacgcgc gtccccatgc agtacgtccg cgacaacgaa attatgctca acatcggcgc
     2641 aaccgccacg caaaaccttc gaatcgacaa cgattggatc agcttttccg cccgtttcgg
     2701 cggacaagcg cacgacatat ggatacctgt cggacacgtc ctcagccttt tcgcacggga
     2761 gaccggagaa ggtatggggt ttgaattgga ggagtaccgc cccgacacgc cgtctgaaaa
     2821 cacctctgcc gaaaccgcgc cccggcccgc caaaaaaggt ttgaaattgg tcaaataaat
     2881 ctatgccgtc tgaacggaat cgtgtttcag acggcatttt gtccgatggg gcgcaaacgg
     2941 aatccgtttg tcggcaaaac ctgtttcggc gtatcgaaac cgtgttgccc ctgccgattc
     3001 gatattggga cattgaaaat gccgtctgaa cctgcgatac gggcttcaga cggcgttttg
     3061 tccgatattc gggcaatcag gcggtcagta cggttttcaa gattttgttg acttcgccca
     3121 tatcggcttt gccggcgagg cgggttttca atacgaccat cactttgccc atatccgcca
     3181 tacctgccgc gccggtttcg gcaacggctg cttcgacggc ggtgcggatt tcgccggcgg
     3241 agagcatttg cggcaggtag cggtgcagca cgtcgatttc ggcgttttct ttgtctgcca
     3301 aatcctgacg gccggcttca gtgtagattt tcgcgccgtc tttgcgctgt ttgaccattt
     3361 tggtcaggat ggcggtgatt ttggcatcgt cggcttcgat gcgttcgtct acttcaaact
     3421 gtttgacggc ggcattgatg aggcggatgg tgccgaggga aacttgatct ttggcgcgca
     3481 tcgcggtttt catatcttcg gtaaggcggg ttttcaggct catgatgtcc tcgctgggat
     3541 gtcggatgga aacggcgggt ttcgatgccg tctgaaaagc aaaacacacc gcagagcagg
     3601 tcttgcggtg tgtcttcata tcacagggct gacctgtaat ctgtacttga acgtttagta
     3661 cattttgggc ggcagctgtt ggctgcgcag gcgtttttgc aggcgtttta cggctgccgc
     3721 ttttttgcgt ttgcgttcgg tagtcggttt ttcgtaggct tcgcgggcgc gcagctcggt
     3781 cagcaggccg gttttttcta cggcgcgttt gaaacggcgc atagcgactt caaagggttc
     3841 gttctctttt acgcggattg caggcatttt atttccttta ataaattcgg ttgttccatc
     3901 tgcccatcat aatcggtgga aagggtaggg cagacggtgt tgtaggtttt ccgtatcgct
     3961 gtgcctttcg gcggcgggat acgcgccgtg acggaaacat cacctcctaa cgggtcggct
     4021 tgtgccgtgt caagctgctt gaaaaagtag gaaaaacaat tttcggattg tcttatattt
     4081 atggttcgcc gtcaatgccg ttcgggataa aaatgccgtc tgaaagaccg ggcgggtttc
     4141 agacggcatc ggcacgtcag cgtgcgggaa cggtgcccat acgctgtttg agcgggatgt
     4201 gcggcgcgcc gaagagggag gcgtagtagg cggcattggt catcactttt ttgacatagt
     4261 cgcgcgtttc ggaaaacggg atggtttcgg catataccgc gccttcgagg ggtgtgtccg
     4321 cctgccatcg gcgcgccctg ccggggccgg cgttatagcc tgcggtggcg aggatttcgt
     4381 tgttttgcag gcggcgtttg gtgtccgcca tataccacgt ccccatacgg atattgccgt
     4441 cggcggtgta gagctgggcg gcatccatac cgattttgcc ggcgatttcg cgcgcggtgg
     4501 caggcataac ctgcatcagc ccctgcgcgc ctacgcggga ttgcgcgcct ataacgaagc
     4561 ggctttcctg acgaatcagg ccgtaaaccc aagccggatc gacattaaca ttttgcgcgt
     4621 ggcggattac cgtgtcttta aacggcgaaa tatagcgcaa ggtgtagttg agtttgcggt
     4681 cggtgcgttc cgcgctgttg accgccatat cgtaaaaacc gtggtcgaac gcggtttgcg
     4741 cggcggccag cagcttgtct tcgtcaaagc cgcgtgtggc aaaacgccat tccgcctgag
     4801 cctgacggcg cattttcgca tcgccggcgg ttcggctgtg ttggaacaac accagtgcgc
     4861 gcttaatcgc gccgtcttcc gccatacgga ggacgctgtt tttgccggca tcgggaacat
     4921 tgttgcgcgt atcgattttg cgccccaact cttcccctgc cagtacggca taaaaattcc
     4981 tgcccgtcgc tgccgcctgt ttgtaaagtt tttccgcttc ttgcgtgttg cccgttgcgg
     5041 cgcggctgcg tgccagccag tagagccagg tcgggctttt ttgcagtttt tcgggcatat
     5101 gcgagatcac ggaggccagc tcgtcccaac gtcgggcgcg caaggtggcg cgggcgtacc
     5161 actcgatttg gtcgtcggtc agttggcggc ggtcggcaac cttgccgtaa tagtccaagg
     5221 cggcaggcac attgaggctt tgcgactgat aatgccccaa tacgccccac gcgaaactgc
     5281 gttgttcggg gcttaaaccg ctttccattt cggacagcag ggcggcggca ttcggcgatt
     5341 tgcgtgcttc tttgccgatg acgttcaaca gggcatattc gcgcgaacct tgtgtaccgc
     5401 cgtcaaacgg gctgcccaat gcggcggcaa ggttgcgcgc gtccgtcgtt tggcggccgg
     5461 ccagcagtcc gcgcacgcgc ctccaggcat cgttgccgtc caacaagccg gatgcggctg
     5521 cctgttccaa cagtttggtg cagcccgaag gcagtttgcc cgtatttttg accagttcgg
     5581 cggcacgcgt atagtcgttg cggctcgaat cggcgtagca ttcgacttct tgggcgcgcc
     5641 cttcaggttt gagtttggca tattcctgtg caaacagcgt ccactgtctg cgcgcgccca
     5701 aagacttcag ccactcgttg cggacatttt ccgccatcgc gctgtcgccg gcgttttcca
     5761 aataggcggc ggctgccgca tcgttgttct gtttcactgc atccagtgcg gacgggtagc
     5821 cgccgtaatc tgccagcgtt tttccttccg gttcggcagg gcgggtggga acgcttgccg
     5881 aaaggtcggc agtttctata ttgtctgccg gggtcttgcc ggctggcagt gtgtttgtcg
     5941 aagaacacgc ggcaagcacc agggccgcca gcagcggcag ggaatgcttc atagagggta
     6001 ggtacatcgg atttccttaa gaatcggaac cctgaacggt cagggttgga aaagacaaaa
     6061 tgccgtctga acaggcgttt gcccgaatta tatgccgaaa ctgcaccgtc tttggaatgt
     6121 ttccgacata atttatattt ttcaatcatt tgccgtttgg gtgcgaaccg ctgcctttgc
     6181 ccgtttcaga cggcattgtc cgaaatggtt gcccgcttcc tgctttattg acaaaaaaac
     6241 gcttttccga taatatccta cgaaaattaa cctgccgatt tgacacagct tgcgggcata
     6301 acagctaaag cgttccgaca atttcagctt tatcttccgc gcccgttgtg tccgacatcg
     6361 ggctttgttg tatgggaaag acaatgatta ttttggacaa ggtttccaag cattaccaaa
     6421 cgcgcgacaa aacccgtttt gccgccgtcg agccgaccag cctcgaaatt caagacggcg
     6481 aaatcttcgg gctgatgggc tattccggcg cgggcaaatc cactttgttg cgcctgatca
     6541 acctgttgga acgccccgac acgggcaaag tcaacgtctg cggacaagaa ctgaccgcgc
     6601 tcgatgccgc cgcattgcgt caggcgcggc agaatatcgg catggtgttt cagcagttca
     6661 accttttaag caaccgcacc gtcgccggca acgttgcctt tccactggaa atcgccggat
     6721 ggccgtctga aaaaatcaaa gcgcgcgttg ccgagtgcct cgaaatcgtc ggcttgaccg
     6781 aacgcgccgg ccactacccc gcccagcttt caggcgggca gaaacaacgc gtcggaatcg
     6841 cccgcgcgct cgcgcccaag ccgcaagtca tcctcgccga cgaacccact tccgccctcg
     6901 accccgccac cacgcgcagt gtcttggaat gtttggaaga catcaacaaa cgcttcaacg
     6961 ttaccatcgt catcgtaacc cacgaaatga gcgtcatcag acgattgtgc gaccgcgccg
     7021 ccctgttgga taaagggaaa gtggtggaaa tcgtcgaagt acgcggcaac caaatccacg
     7081 ctcaatccga aatcgggcgc gaactgattc gggaggactg atatggcaga cttaacattc
     7141 gaacaagccg tttccaccat cgtcggcatg aaagacgaaa tcgtccgcgc cttgggcgaa
     7201 actttcgtga tggtcggctt gtccaccaca ttcgccgtca tcttcggcac gctcttgggc
     7261 gtgttgctct tcgtaacttc cagccgccaa ctgcattaca acaagccggt aaacttcctg
     7321 ctcgacaacc tcgtcaacct catgcgcgcc ttccccttcg tcatcctgat gattgcgatg
     7381 atacccgcca cacgcgccat cgtcggcagc accatcggcc cggttgccgc ctcgctggtg
     7441 ttgagcgtgt cgggattgtt ttattttgcc cgactggtgg aacaaaacct gcgcgaagtc
     7501 ccaaaaggtg tgattgaagc cgccgccgcg atgggtgcgc cgccgattgc catcgtcctc
     7561 aaagtcctct tgaacgaagc gcgcgcgggc atggtttcca gcattaccgt gcttgccatc
     7621 gggcttttgt catacagcgc ggcggcaggc atgataggcg gcggcggctt gggcgacctc
     7681 gccatccgct acggctacta ccgctaccaa accgaagtca tcgtcttcat cgtcgccctc
     7741 ctcgtgctgc tggtcatcct gattcaaagc accggcaacg cgttggcgcg gaaactcgac
     7801 aaacgttaaa cccgaatgcc gtctgaacgc caaaaacccc gccgctatcc gaaaaatgct
     7861 ataaaatccc cctgttcgcg gcaaatgccg tctgaacgcc gaatccggac ggcaggactc
     7921 cctgcctgtc atttttgttt gaaaccgcca caacatcagg agaaaatatg aaaaccttct
     7981 tcaaaaccct ttccgccgcc gcactcgcgc tcatcctcgc agcctgcggc ggtcaaaaag
     8041 acagcgcgcc cgcagcctct gccgccgccc cttctgccga taacggcgcg gcgaaaaaag
     8101 aaatcgtctt cggcacgacc gtcggcgact tcggcgatat ggtcaaagaa caaatccaag
     8161 ccgagctgga gaaaaaaggc tacaccgtca aattggtcga atttaccgac tatgtgcgcc
     8221 cgaatctggc attggcggag ggcgagttgg acatcaacgt cttccaacac aaaccctatc
     8281 ttgacgattt caaaaaagaa cacaacctgg acatcaccga agccttccaa gtgccgaccg
     8341 cgcctttggg actgtatccg ggcaaactga aatcgctgga agaagtcaag gacggcagca
     8401 ccgtatccgc gcccaacgac ccgtccaact tcgcacgcgc cttggtgatg ctgaacgaac
     8461 tgggttggat caaactcaaa gacggcatca atccgctgac cgcatccaaa gccgatatcg
     8521 cggaaaacct gaaaaacatc aaaatcgtcg agcttgaagc cgcacaactg ccgcgcagcc
     8581 gcgccgacgt ggattttgcc gtcgtcaacg gcaactacgc cataagcagc ggcatgaagc
     8641 tgaccgaagc cctgttccaa gagccgagct ttgcctatgt caactggtct gccgtcaaaa
     8701 ccgccgacaa agacagccaa tggcttaaag acgtaaccga ggcctataac tccgacgcgt
     8761 tcaaagccta cgcgcacaaa cgcttcgagg gctacaaata ccctgccgca tggaatgaag
     8821 gcgcagccaa ataaggcagt cgtataaaat gatgccgtct gaaccgcatt ccgtgttcag
     8881 acggcatttt tgtcctttaa tccgccattc cctgccattc cgccaaatcc ggcgtatcga
     8941 ttccgaacag cgacagcgtg tgtgcgatac tgtgcgccac tatgtcgtcc gccgtctgcg
     9001 gcttgcggta catcgcagga acagggggaa acaccacgcc gcccatttcc gttacccgct
     9061 tcatattgtc caaatgggca aggttcagcg gcgtttcgcg caccatcagc accagccgcc
     9121 gcctttcctt caaaaccaca tccgccgcac gcgtcaagag attgtcgccg aagccgtgcg
     9181 cgacagaggc aagcgtccgc atcgaacagg gtgcgaccag catcccgtcc gttttaaacg
     9241 taccgctggc aatgcacgcc ccgatattgc cgatcggatg gacgaaatca gccaaggcat
     9301 atacttcgtc tttcgtataa tccgtttccg aagcgcgcgc catctccgcg cctttcgata
     9361 ccacaaggtg cgtttcgaca tcttgcgcgc gcaaaagttc caaagccttc acgccgtatt
     9421 ggaaaccgct cgccccgctg atgccgatta tcaaacgccg taccatcatc cgcctttccc
     9481 ataaaaccgc ctgcaacggc aaaccggcta ttatagtgaa aaaacagaaa tccgataaac
     9541 acggatacaa attgtcggca acacccaata tccgataaaa tacccgattt aacatcctat
     9601 ctgaataggc acgggagggc ggtatggcaa aagtaaaagg cggattgggg cgcggcttgg
     9661 attcgctgct cgccaacggt gcggacaaca gcagcggcga ccggctgact gcggttgcgg
     9721 ttaaagatat ccggcccggc cgctatcagg cgcgcgtcca aatcgatgac gaagccttgc
     9781 aggaactggc agattcgatt aaggcgcaag gcgtgataca gcccgtcatc gtgcgcgaac
     9841 acggactgtc ccgatacgaa ctgattgcag gcgaacgccg ttggcgcgcc gctcagattg
     9901 ccggcctgtc cgaaatcccc gccgttatca aaaccatcag cgacgaaacc gcattggcaa
     9961 tgggtttgat cgaaaacctc cagcgcgaaa acctcaaccc catcgaagaa gcacaaggct
    10021 tgaaacgcct tgccgacgag ttcgggctga cccacgaaac catcgcccaa gccgtcggca
    10081 aaagccgaag cgcgatttcc aacagcctgc gccttttaag cctgcccgaa tccgtgcagg
    10141 aaatgcttta ccaacgccgc ctcgaaatgg ggcacgcccg cgcactgctg accctgcccg
    10201 tcgtcgaaca gctcgaattg gcgcaaaagg ccgtcaaaaa cggctggtcg gtgcgcgaag
    10261 tcgaacgccg cagccaggcc gcccttcaaa acaaacgtcc cgagccaaaa aaaactgccg
    10321 ccgccgacat cggccgcctg aatgatttgc tgactgaaaa attgggtgtc aacgccgaaa
    10381 tcaagaccgc caaccataaa aaaggcaaaa tcatcctgca tttcgacacg cccgaaacct
    10441 tcgaccatat cttgaagcag ttgggcatag attaccggcc ttaattttgc gggatatgcc
    10501 gtttgaaata tagagaatag ctttccagat tttaagtggg aaatataatt ctattgacat
    10561 tttttctgct tcgcgtaaga atcgttttcc tgttttcatt tttaattttc gaggaaatta
    10621 tgaacacacg catcaacttt accgcaactg tgggtaagcg cgtgagccaa tgctatgtta
    10681 ccagtgtaat cagcacaatc ggcgttacca cttccgatgc aatttgttcg ggaggcggaa
    10741 cgaacaaagc caaaagtcaa tgcaatgctt tgcttaaagc ggcaggccgc tgctaatcct
    10801 ttattcggaa aaggtcgtct gaaaatattt tcagacgacc ttttatttat tgagcaaact
    10861 ccccaaactg ccgacaatcc gaccgataaa ctactacaat ttcgcgttga ttttttatta
    10921 tttccacgta aagcaaacaa atcacgtaag aacattaaaa gtgtaaagaa attgcggaca
    10981 aactccttaa tatcctgtaa tcatatgtaa tatgtgttag aatagcggag ttggattttg
    11041 gggagtcgct cgtctgtatc ggcggcgagt ttcacaaaga ttaacatatt gcttgacgta
    11101 ataaggcttc taaattatat tagccgcgcc ccaaacctga ggcggaaaca ttccctttat
    11161 gaagcagatt atcatcctac aatccgccgt attgtccata tgcgccgcag ttgcctttgc
    11221 cgtatggggt tttgccggat tcctttcggc ggtcgggggc ggtttgtcct acctgcttcc
    11281 cacgtttgtt gcagttttac ttctaaaact tttcagggga aaccccttcc tgcaaagcag
    11341 gatgttcgtc ttcggagaga ttttaaaagt agtgctgtcg ctgttgtcca tgcttgccgt
    11401 atttgcaata tggcatcaat cgctggtgtt cgtcccgttt ttgatggggc tgctcggtgt
    11461 cagccatctg gtttttttag tattgttgag agtgaaagat tatggcaggt gaaaccatta
    11521 ccgctgccga ctacatcaag caccacttgc agagcttgac cagtttgtcg gatgttactc
    11581 agggtcaggg actgaaaaac attgctgatt tttcgtttat taaccttgat gccgtttttt
    11641 ttgccgtcct gttgggcgta atcggcagct tcctgttgtg gcgcggtgca aaaaaagcaa
    11701 cggcaggcgt tcccggacgt ttccaggccg ccgtggaaat cttgttcgag tttgtggacg
    11761 atatgtgtaa gagcatcatt cacagcgaaa agtctagaaa agccgtcgcg ccgttgggtc
    11821 tgacgctgtt tgtttggatt ttcctgatga acgcgatgga tatgctgccg gtcgatttgc
    11881 tgccgatggt atggcagggt attaccggca accatcacgc cctgctgcgc atcgtaccga
    11941 ctgccgattt gaacaccgct ttggcactcg ctgtcggcgt gttgctgatt tgtatctatt
    12001 acaacatcaa aatcaaagga ttgggcggct ggtttcacga gttgttcagt gtgccgttcg
    12061 gtgcaaaact cgcacctgcg aactttctgt tgaacttggt cgagtttctt tctaaaaccg
    12121 tatcccacgg tatgcggttg ttcggcaata tgtatgcggg cgagctggta ttcctgctga
    12181 tagccttgct tggcggtgct tgggcggctt ccggcagcgt cgaagtcatg gatccgattc
    12241 tgtttgtatt ccacattatt gccggtttgg catgggcgat tttccatatt ttggtgatta
    12301 ccctgcaggc atttattttc atggcgttgg cgttcgtcta tatcgggcag gcgcacgatg
    12361 cccactgatt ttcccaagta gtttgttttt gtagtttaac ctttgtttct ttaaggagtt
    12421 taaaatgggt ttgattgcta tcgcatgtgg tttgatcgtt gcattgggtg cattgggtgc
    12481 atctatcggt atcgcaatgg tcggttctaa atatttggag tcttctgctc gccaacctga
    12541 actgatcggt ccgctgcaaa ccaaactgtt cctgattgcc ggtctgattg atgccgcatt
    12601 cttgatcggt gtcgccattg cactactgtt cgccttcgtt aacccgtttg caggtgcata
    12661 atcaggacgg attcgtccgt tcgtacaggt gcagcctgtt tgattaaccc caatacgaag
    12721 gttaagtaac gtgaatatca atgcaacatt attcgctcaa atcatcgtct ttttcggttt
    12781 ggtatggttt accatgaaat ttgtgtggcc gccgattgca aaagctttgg atgagcgtgc
    12841 cgcaaaaatc gccgagggct tggctgccgc cgagcgtggt aaaagcgatt tcgagcaggc
    12901 tgaaaaaaag gttgcagaac ttttggcaga agggcgtaat caggtttccg aaatggttgc
    12961 caacgccgaa aaacgtgccg ccaaaattgt cgaagaagcc aaagaacagg cttcttccga
    13021 ggcggcgcgc attgcagctc aggcaaaggc tgatgtggag caggaattgt tccgcgcacg
    13081 cgaatccctg cgcgatcagg ttgccgtgtt ggctgtcaaa ggtgccgaat ctattttgcg
    13141 cagcgaagtc gatgcttcca aacacgcaaa actgctcgat accctgaaac aggagttgta
    13201 atcttatggc agagtttgca acgatcgcca gaccttatgc aaaggcattg ttcggtctgg
    13261 ctcaggaaaa aaaccaaatt gagtcttggt tgggcggact ggaaaaactt gcggcggttg
    13321 ttcaggaagg gaaggtggct tcattgattg accgtcctga aacgaatgct tcagaaaaag
    13381 cagatatcct catcgatttg gtcggtttga aagacaagga gttaaaaaac tttgttatcg
    13441 tcttggccgg gcagaaacgt ttgtcgatat tgccggaagt gtatgctcaa tatcaagact
    13501 tgaccttatc attcaaccat atcaaatctg ccgtcattta cagtgcctat ccgttgaccg
    13561 acaaacaggt cggcgagttg gcgcaaatgc tgaataagcg tttcgacagc gagctgaaaa
    13621 tctctgtcga aatcgaaccg gagctgattg gcggcataaa agttgaagtg ggtgatcagg
    13681 ttttggattt gtctgtacaa ggcaaactga gtgctttgta cacgactatg acgaattagg
    13741 agagttttca tgcagcttaa tcctgctgaa attagcgatc tgattaaagc caagatcgaa
    13801 aatctgtctg taaatgccga agtgagtacc cgtggtacgg tgatttcggt aactgacggt
    13861 atcgttcgca tccatggttt gtcagatgca atgcaaggtg agatgctcga attcccgggt
    13921 aacactttag gcttggcgat gaacctggag cgcgactccg tcggtgccgt agtgttgggc
    13981 gagtacgaac atattaaaga aggcgacacg gttacctgta ccggccgtat tttagaagtg
    14041 ccggtcggac gcgaattggt cggacgcgtc gtcgatgcat tgggtcggtc tatcgacggc
    14101 aaaggcccga ttaatacaac tttgaccgcc cctgtcgaaa aaatcgcacc gggcgtgatt
    14161 gcacgcaaat cggttgatca gccgatgcaa accggtctga aggcgattga ctctatgatt
    14221 cctgtcggtc gcggtcagcg tgagttgatt attggagacc gtcagacagg taaaacggcc
    14281 gtggcattgg atgccatcgt caaccaaaaa ggtacgggtg ttatctgtat ctatgtcgct
    14341 atcggtcaaa aagcatcttc tattgccaac gtggtccgca aattggaaga gcatggcgcg
    14401 atggaacaca cgattgtggt tgctgcaact gcatctgaag cggcggcatt gcaatatatc
    14461 gcaccttatt caggttgtac gatgggtgaa ttcttccgcg atcgtggcga agatgccttg
    14521 attgtttatg acgatttgtc caaacaggct gtggcttacc gtcaaatttc cctgcttttg
    14581 cgccgtccgc ccggccgcga agcctatccc ggcgatgtgt tctacctgca ctcccgtctg
    14641 ttggaacgtg cggcacgtgt caatgaacac gaagtggaga aattgaccaa cggcgaagta
    14701 aaaggcaaaa ccggttctct gaccgcgttg ccgattatcg aaacccaagc gggcgacgta
    14761 tctgctttcg ttccgactaa cgtcatttcg attaccgacg gtcagatttt cttggaaacc
    14821 gacctcttca acgccggtat ccgtcctgca atcaatgccg gtatttccgt atcccgcgta
    14881 ggcggtgctg cacaaaccaa agtgattaaa aagctgggtg gcggtatccg tttggcgttg
    14941 gcacaatatc gtgaattggc ggcgttctcg caatttgcat ccgatttgga tgaagctacg
    15001 cgcaaacagt tggagcatgg cgaagtcgta accgaactga tgaaacagaa acagttcagt
    15061 acgttgaata cggctgaaat ggctttgacc ctttgggcaa tcaacaacgg ttcgtattct
    15121 gatgttccgg ttgccaaagc tctagctttt gaatctgaat ttttgagctt tgtccgtacc
    15181 caacatccgg aagttttgga agccgtcaat gcttcaggtg caatgtccga cgagagcgag
    15241 aaaacgcttg aagcagccat gaaatccttc aaatcttctt acgcctatca ggcataaggc
    15301 ttgaagtgaa aggagtctga aatggcagta ggaaaagaga ttctcaccaa aatccgcagt
    15361 gttcagaata cccaaaagat cactaaagcg atgcaaatgg tgtcaacctc taaaatgcgg
    15421 aagactcagg aacggatgcg tttggcgcgt ccgtatgccg aaaaagtgcg tatggtgatg
    15481 agccatcttg cgcaaaccaa taccgatcat ggtattccgt tactggaatc tcatcgggaa
    15541 atcagacgtg tcggttttat tttgattacg tctgataagg gtttgtgtgg cggtttgaac
    15601 gccaacgtgc tgaaaaagtt tttggcacaa gttcaagagt atcggaatca aggtattgaa
    15661 gaggaaatcg tatgccttgg cagtaaaggt ctgatggcgt gtcagagcat tggtctgaat
    15721 gtggttgcca gtgccgtaaa tttgggcgat accccaaaaa tggaaatgct gctcggacct
    15781 ttgacagaac ttttccaacg gtatgagaaa catgaaattg acagaatcca tctggtgtat
    15841 tcgggttttg tcaataccat gcgtcaagaa ccgaggatgg aagtattgct gcctatcggt
    15901 gagaacgtga ttggcgattc agctcccaaa tcaccgttca gctgggaata ccgctacgaa
    15961 ccgactgcac ttgcagtgtt ggaatatctg gttcgccgct atttagagtc tgtggtttat
    16021 caggcgttga gcgacaatat ggcatccgaa caggcagccc gcatggttgc catgaaagct
    16081 gcaacagaca atgcaggcaa tgccatcaaa gagttgcgtc tggtatataa caaatcgcgt
    16141 caagctgcga ttaccacgga attgtcagaa attgtagcag gtgcggcggc cgtctgatgc
    16201 cgtctgaatc ctgtacggaa attaggatac gataatgagc caaggcaaaa tcgtacaaat
    16261 tatcggtgcg gttgttgacg tggaatttcc acgcgacatg attccgcgcg tttacgacgc
    16321 tttgaaatta gacgaaaacg gtctgacttt ggaagtccaa cagcttttgg gtgatggcgt
    16381 agtccgtacc atcgctatgg gcagctcgga cggtttgaaa cgcggcatga ctgtgagcaa
    16441 tactggttcg cccattactg tgccggtagg taaaggtacg ttgggacgca ttgtcgatgt
    16501 attgggaact cctgttgacg aggcaggtcc aattgatacc gacaagagtc gtgccatcca
    16561 ccaagccgct cctaagtttg acgaactgtc ttccacaacc gaattgctcg aaacgggcat
    16621 taaagtgatt gacttgctgt gtccgtttgc caaaggcggt aaagtaggtc tgttcggcgg
    16681 tgccggtgtg ggtaaaaccg tgaacatgat ggaattgatc aacaacatcg ccaaagcgca
    16741 cagcggcttg tccgtgttct caggcgtggg tgagcgtacc cgcgaaggta acgacttcta
    16801 ccacgagatg aaagattcca acgtattgga taaagtagcc atggtgtatg gccaaatgaa
    16861 cgaacctcca ggcaaccgtc tgcgcgttgc tttgaccggt ttgactatgg ctgaatactt
    16921 ccgtgacgaa aaagacgaaa acggtaaagg tcgtgacgta ttgttcttcg ttgacaacat
    16981 ctaccgttac actctggccg gtaccgaagt atccgcactg ttgggccgta tgccttctgc
    17041 agtgggttac caaccgacat tggctgaaga aatgggtcgt ttgcaagagc gtattacctc
    17101 tacccaaacc ggttccatta cttccatcca agccgtatat gtacctgcgg atgacttgac
    17161 tgacccgtct ccggcgacaa ctttcgccca cttggacgcg accgtcgtat tgagccgtga
    17221 tattgcctct ttgggtattt acccggcagt tgacccgctt gactctactt cgcgccaatt
    17281 ggatccgatg gtattgggtc aagagcacta cgacgtagcg cgcggtgtac agtctactct
    17341 gcaaaaatac aaagaattgc gcgacatcat cgccattctg ggtatggacg aattgtctga
    17401 cgaagacaaa ctggctgtaa tgcgtgcccg taaaatccaa cgcttcctgt ctcaaccgtt
    17461 ccacgttgcc gaagtgttta caggttctcc aggcaaatat gtcgccctgc gcgataccat
    17521 tgccggcttc aaagccatct tgaacggcga atacgatcat ctgcccgaac aggcattcta
    17581 tatggtcggc agcatcgaag aagcggttga gaaagcgaaa accttaaact gaggaggtcg
    17641 gcatgagcat catgcaagtt gaggtggtaa gtggcgagca gaaaatctat tcaggcgagg
    17701 caacatttat cgttgttccg actgtacagg gcgaactcgg tatttatccg cgacacgagc
    17761 cgattatgag tttggtgcgg ccgggggctt tgcgtttgac cgttccgggc gaggataaag
    17821 aggttttggt tgctgtttcc ggcggtgttt tggaagtaca gcctgataaa gtaactgtct
    17881 tggcggatgt tgccgtccgc agtgcagaga tggatcaggc acgtgcggaa gaggcgaaaa
    17941 aagccgcaga agcgggtatt tcccaagcta aagacgataa ggctttggcg gaagcacata
    18001 aagcattggc tgctgcgatt gcacagctca aaactttgga ctatatccgt tcgcacaaga
    18061 aataaacttg tttggtttga aaaagcacga ccgtttggcc gtgcttttta ttttccggat
    18121 ttaaattaaa aaattatcca tcccctggtt tcaaatcagg ctgtcttcga gataaatcta
    18181 ttcaagcggc tatggaaaca gtaaaataaa acctttattt tatcgaacaa taagccatgc
    18241 ttaccttcca acaaatcatc ttcaaactgc aaaccttctg ggcagacaaa ggctgcaccg
    18301 tcatccaacc cttcgacatg gaagtcggtg ccggcacatc ccatcccgcc acctgcctgc
    18361 gcgcgctcgg ccccgagcct tggtttgccg cctacgtcca acccagccgc cgccccaaag
    18421 acggccgcta cggcgacaac cccaaccgcc tgcaacacta ttaccaattc caagtcgccc
    18481 tcaaacccgc ccccgccaat atccaagacc tctatctcga ctccctgcgc gaattgggca
    18541 tcgatcccaa agtccacgac atccgctttg tcgaagacga ctgggaaaac cccaccctcg
    18601 gtgcgtgggg tttgggctgg gaagtctggc tcaacggcat ggaagtaacc cagtttacct
    18661 atttccaaca agtcggcggt atcgattgca cgcccgtact cggcgaaatt acctacggca
    18721 tcgaacgctt ggcgatgtac ctgcaaggcg tggaaaacgt ctatgacctc gtttgggcaa
    18781 aaacgccgga cggcaatacc gtaagctacg gcgacgtgta ccatcaaaac gaagtcgagc
    18841 aatctaccta caacttcgaa tacagcgatg ccgactggct gctgcgccag ttcaacgact
    18901 acgaagcgca agccaaacgc ctgttcgccg aagaaaacgc cgaccttgcc ttacctgcct
    18961 acgagctggt cctcaaagcg gggcatacgt tcaacctttt agacgcacgc ggcgcgattt
    19021 ccgtaaccga gcgggcaact tatattggac gtattcgtgc attgagccgc gccgtggcgc
    19081 aaaaatatat cgaaagccgt gagaaactgg gcttcccgtt gattaaaaaa taacgatacg
    19141 tcgtctgaag caggttttca ggcgaccttt attgcgtatc cattaataca ggccgtacgg
    19201 catatgcagg ctttataagc atggcctacg gacaaacaga aagtctcaca tgacaaccca
    19261 aaccctttta atcgaactcc ttaccgaaga actcccgcca aaagccctga ataatctggg
    19321 caaccatttt gccgcttccg ttgccgaagg cttggaaaaa gcgcaactgg ttgacggcgc
    19381 ggccgaattt acggcttatg cctcgccgcg ccgtttggcg gttcaagtca aaaacgtcaa
    19441 agctgttcag gccgatcaga aaatcgtgaa aaaaggccct gccgtggcga atgccgtaaa
    19501 agacggtacg ccgaccaagg ctttggaagg ttttgcgcgc ggggcggggg cgaaaatcga
    19561 agacctgacc atcatccacg acggcaggca ggacgtgtac gcctacgaat acgtccaaac
    19621 cggcagaccg ttgggcggac ttttggaaga cattatcaat caagctgtta agaagctgcc
    19681 gattccgaaa gtaatgcgtt ggggcagcag cacgtttacc ttcgtgcgcc ctgttcacgg
    19741 gctgattgtg ctgcacggcg gcgatgtcgt gaacgtcagc gttttgggcc tgcaaagcgg
    19801 caatcaaacc ttgggacacc gctttctttc cgacggcgaa atgatcattg aaaacgccga
    19861 cagctacgcc gcacaaatgc gcgggcaagg caaagtcgtc gcttcgtttg ccgggcgcaa
    19921 agccgcgatt cagacggcat tggaagggca ggcacgccgt ctgaacgcga ccgttgccgc
    19981 cgatgaagcc ctgttggacg aagtgaccgc gctggtcgaa tggcctgttg ttttggaagc
    20041 cggttttgag gaacacttcc tcgccgtgcc tcaagaatgc ttgattttga cgatgcagca
    20101 aaaccaaaaa tacttcccgc tgctcgacca aaacggcaag ctgatgaacc gcttcctgct
    20161 ggtttccaac ctgcaaaccg aagacccgtc gcacatcatc cgaggcaacg aacgcgtatt
    20221 gcgcgcgcgc ctgtctgatg ccgagttctt ctacaaacaa gaccaaaaag cgaccttgga
    20281 aagccgcctg cccaagctgg cgaacgtggt gtaccacaac aaaatcggtt cgcaggccga
    20341 acgcatcgag cgtctgcaaa gcatcgccgc ccacatcgcc aaagcattgg gcgcggatgc
    20401 cgcggcagcc gagcgcgccg cgcgtcttgc caaagccgac ttggtaactg aaatggtcgg
    20461 cgagttcccc gaactgcaag gcacgatggg caaatactac gcccgcttgg acggcgaaac
    20521 cgaagaaatc gccgaagcta ttgagcaaca ctaccaaccg cgttttgccg gcgacaagct
    20581 gcccgaaagc aaaattgccg ccgccgtggc acttgccgac aaactggaaa ccctggtcgg
    20641 catttggggc atcggtttga ttcctaccgg cgacaaagac ccctacgccc tgcgccgcgc
    20701 cgccttgggt attttgcgga tgctgatgca gtacggtttg gacgtgaacg aactgattca
    20761 gacggcattc gacagcttcc cgcaaggttt gctcaacgag aaaacgccgt ctgaaaccgc
    20821 cgactttatg caggcgcgcc ttgccgtgtt gctgcaaaac gattatccgc aagacatcgt
    20881 tgccgccgta ctcgccaaac agccgcgccg tttggacgat ttgaccgcca aactgcaagc
    20941 cgtcgccgta ttcaaacaac tgcccgaagc cgccgcgctc gccgccgcca acaaacgcgt
    21001 gcaaaacctg ctgaaaaaag ccgatgccgc gttgggcgaa gtcaatgaaa gcctgctgca
    21061 acaggacgaa gaaaaagccc tgtacgctgc cgcgcaaggt ttgcagccga aaattgccgc
    21121 cgccgtcgcc gaaggcaatt tccgaaccgc cttgtccgaa ctggcttccg tcaagccgca
    21181 ggttgatgcc ttcttcgacg gcgtgatggt gatggcggaa gatgccgccg taaaacaaaa
    21241 ccgcctgaac ctgctgaacc gcttggcaga gcagatgaac gcggtggccg acatcgcgct
    21301 tttgggcgag taaccgttgt acagtccaaa tgccgtctga agccttcagg cggcatcaaa
    21361 ttatcgggag agtaaattgc agcctttagt cagcgtattg atttgcgcct acaacgtaga
    21421 aaaatatttt gcccaatcat tagccgccgt cgtgaatcag acttggcgca acttggatat
    21481 tttgattgtc gatgacggct cgacagacgg cacacttgcc attgccaagg attttcaaaa
    21541 gcgggacagc cgtatcaaaa tccttgcaca agctcaaaat tccggcctga tt
//
LOCUS       DAXFWM010000033        21580 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000033 DAXFWM010000000
VERSION     DAXFWM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21580
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.033"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            198..1541
                     /locus_tag="ACFI8B_001167"
     CDS             198..1541
                     /locus_tag="ACFI8B_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC3834402.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEKVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1544..2776
                     /locus_tag="ACFI8B_001168"
     CDS             1544..2776
                     /locus_tag="ACFI8B_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC3834403.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2769..3545
                     /locus_tag="ACFI8B_001169"
     CDS             2769..3545
                     /locus_tag="ACFI8B_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC3834404.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3545..4171
                     /locus_tag="ACFI8B_001170"
     CDS             3545..4171
                     /locus_tag="ACFI8B_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC3834405.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4175..4768
                     /gene="nqrE"
                     /locus_tag="ACFI8B_001171"
     CDS             4175..4768
                     /gene="nqrE"
                     /locus_tag="ACFI8B_001171"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC3834406.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4782..5999
                     /gene="nqrF"
                     /locus_tag="ACFI8B_001172"
     CDS             4782..5999
                     /gene="nqrF"
                     /locus_tag="ACFI8B_001172"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC3834407.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6516..7571
                     /locus_tag="ACFI8B_001173"
     CDS             6516..7571
                     /locus_tag="ACFI8B_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC3834408.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGQTEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7597..7812
                     /gene="nqrM"
                     /locus_tag="ACFI8B_001174"
     CDS             7597..7812
                     /gene="nqrM"
                     /locus_tag="ACFI8B_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC3834409.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7936..8514)
                     /gene="grpE"
                     /locus_tag="ACFI8B_001175"
     CDS             complement(7936..8514)
                     /gene="grpE"
                     /locus_tag="ACFI8B_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC3834410.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVARKEA"
     gene            8697..9515
                     /gene="cysE"
                     /locus_tag="ACFI8B_001176"
     CDS             8697..9515
                     /gene="cysE"
                     /locus_tag="ACFI8B_001176"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC3834411.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9719..11230)
                     /gene="ubiB"
                     /locus_tag="ACFI8B_001177"
     CDS             complement(9719..11230)
                     /gene="ubiB"
                     /locus_tag="ACFI8B_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC3834412.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11265..11722)
                     /locus_tag="ACFI8B_001178"
                     /pseudo
     CDS             complement(11265..11722)
                     /locus_tag="ACFI8B_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(11719..11865)
                     /locus_tag="ACFI8B_001179"
     CDS             complement(11719..11865)
                     /locus_tag="ACFI8B_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834413.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11882..12220)
                     /gene="erpA"
                     /locus_tag="ACFI8B_001180"
     CDS             complement(11882..12220)
                     /gene="erpA"
                     /locus_tag="ACFI8B_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC3834414.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12353..13039)
                     /locus_tag="ACFI8B_001181"
     CDS             complement(12353..13039)
                     /locus_tag="ACFI8B_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3834415.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13226..13498
                     /locus_tag="ACFI8B_001182"
     CDS             13226..13498
                     /locus_tag="ACFI8B_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834416.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDTEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13706..15634
                     /gene="dnaK"
                     /locus_tag="ACFI8B_001183"
     CDS             13706..15634
                     /gene="dnaK"
                     /locus_tag="ACFI8B_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC3834417.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            15857..17783
                     /locus_tag="ACFI8B_001184"
                     /pseudo
     CDS             15857..17783
                     /locus_tag="ACFI8B_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            17840..18817
                     /locus_tag="ACFI8B_001185"
     CDS             17840..18817
                     /locus_tag="ACFI8B_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC3834418.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLAAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVRGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            19154..19291
                     /locus_tag="ACFI8B_001186"
     CDS             19154..19291
                     /locus_tag="ACFI8B_001186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834419.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            19370..20140
                     /locus_tag="ACFI8B_001187"
                     /pseudo
     CDS             19370..20140
                     /locus_tag="ACFI8B_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20167..20859
                     /locus_tag="ACFI8B_001188"
     CDS             20167..20859
                     /locus_tag="ACFI8B_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3834420.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            21051..>21580
                     /locus_tag="ACFI8B_001189"
     CDS             21051..>21580
                     /locus_tag="ACFI8B_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC3834421.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSR"
BASE COUNT         5640 a         5728 c         5199 g         5013 t
ORIGIN      
        1 tatcaaatca atatccccct tccgacaatt aaaaacggct tccgcaagga aaaaagcggc
       61 aaatctgaca aacaggtaca attcctgata aaatctaaca ctttgctaca ttaatttatt
      121 caatgctttt gatgccacgc gacacggtat caaaaacacg ccgtttttac cttaaccatc
      181 acaagtaatg caaatctatg attaaaatca aaaaaggtct aaatctgccc atcgcgggca
      241 gaccggagca agtcatttat gacggcccgg ccattaccga agtcgcgttg cttggcgaag
      301 aatatgtcgg catgcgcccc tcgatgaaaa tcaaggaagg tgaagccgtc aaaaaaggcc
      361 aagtgctgtt tgaagacaaa aagaatccgg gcgtagtatt tactgcgccg gcttcaggca
      421 aaatcgccgc tattcaccgt ggcgaaaagc gcgtacttca gtcagtcgtg attgccgttg
      481 aaggcaacga cgaaatcgag ttcgaacgct acgtacctga agcgctggca aaattgagca
      541 gcgaaaaagt gcgccgcaac ctgattcaat caggcttatg gactgcgctt cgcacccgtc
      601 cgttcagcaa aatccctgcc gtagatgccg agccgttcgc catcttcgtc aatgcgatgg
      661 acaccaatcc gctggctgcc gaccctacgg tcatcatcaa agaagccgcc gaagacttca
      721 aacgcggcct gttggtattg agccgcctga ccgaacgtaa aatccatgtg tgtaaagcag
      781 caggcgcaga cgtgccgtct gaaaatgctg ccaatatcga aacacatgaa tttggcggcc
      841 cgcatcctgc cggcttgagt ggcacgcaca ttcatttcat cgagccagtc ggcgcgaata
      901 aaaccgtgtg gaccatcaat tatcaagacg tgattgccat cggacgtttg ttcgtaacag
      961 gccgtctgaa taccgagcgc gtggttgcct tgggcggcct gcaagtcaac aaaccgcgcc
     1021 tcttgcgtac cgttttgggt gcgaaggtgt ctcaacttac cgccggcgaa ttggttgacg
     1081 cggacaaccg cgtgatttcc ggttcggtat tgaacggtgc gattgcacaa ggcgcgcatg
     1141 attatttggg acgctaccac aatcagattt ccgttatcga agaaggccgc agcaaagagc
     1201 tgttcggctg ggttgcgccg cagccggaca aatactccat cacgcgcacc actctcggcc
     1261 atttcctaaa aaacaaactc ttcaagttca cgacagccgt caacggcggc gaccgcgcca
     1321 tggtaccgat cggcacttat gagcgcgtaa tgccgttgga catcctgcct accttgcttt
     1381 tgcgcgattt aatcgtcggc gataccgaca gcgcgcaggc tttgggttgc ttggaattgg
     1441 acgaagaaga cctcgctttg tgcagcttcg tctgcccggg caaatacgaa tacggcccgc
     1501 tgttgcgcaa agtgctggaa accattgaga aggaaggctg attatgggct taaaacattt
     1561 tctggaaaaa atcgaaccgc acttcctgcc gggcggcaaa catgaaaaat ggtatgccct
     1621 ctatgaagct gcggcgacga ttttctatac atccggcgcg gtaacgcgca aagcggcgca
     1681 cgtccgcgac gcgctcgact ccaaacgcat gatgattttg gtgtggctgg ctttgttccc
     1741 cgccatgttt tacggcatgt acaacgtcgg cgcacaggca ttcggtgcct taacgcccga
     1801 tttgctgcaa caaagcatcg ccaacgactg gcattacgcc cttgccaacg ctttgggcat
     1861 caatatgtcg cctgaagcgg gcgtgttggg caaaatgctg ttcggcgcga tttacttcct
     1921 gccgatttac gcgaccgtat ttattgtggg cggcttctgg gaagtcttgt tcgcatccgt
     1981 acgcaaacac gaaatcaacg aaggtttctt cgttacttcg attctgtttg ccttaatcgt
     2041 tccgcccacg ctgccgctgt ggcaggcggc tttgggtatt tctttcggcg ttgtggttgc
     2101 gaaagaagta ttcggcggta caggtaaaaa cttcatgaac cctgcgctgg caggccgcgc
     2161 cttcctgttc ttcgcctacc ccgccaactt gagcggcgat gcggtttgga cggcggttga
     2221 cggctattcc ggcgcaaccg cgctggcgca atgggcggca cacggtgcag acggcctgaa
     2281 aaacgccgta accggtcaaa ccatcacttg gatggacgcg tttatcggca aactgcccgg
     2341 ctccatcggc gaagtctcca ctttggcact cttaatcggc ggcgcgttta tcgtgtttgc
     2401 ccgcatcgct tcttggcgca ttattgccgg cgtgatgatc ggtatgattg cgatgtcttc
     2461 gctgtttaac ttcatcggtt ctgacaccaa cgctatgttt gctatgcctt ggtactggca
     2521 cttggtggtc ggcggcttcg ccatcggtat gctgtttatg gcgaccgacc ccgtttccgc
     2581 ttcctttacc aatgtcggca aatggtggta cggcgcgctt atcggcgtaa tgtgcgtgtt
     2641 aatccgcgtg gccaatccgg cttaccccga aggcatgatg ttggcgattc tgtttgccaa
     2701 cctgtttgcc ccgattttcg actatttcgt cgcacaagcg aacatcaaac gcagaaaggc
     2761 gcgcagcaat ggctaagaaa ttcgataaag acagcttcag cggcacgctg attgtcgtgt
     2821 tggcggtcag cctgatttgc tcggtcatcg ttgccggtgc ggtcgtcggc ttgaaaccca
     2881 tccaagaaaa acaaaaactc caagacaaac aaggctatat tttgagcgtg gccggtttga
     2941 tggataagga caccgacatc ggtaaaacct ttgccgagcg tatcgagcaa cgcgtggtcg
     3001 atttggcgac cggcgaatat gtgaaagacg cgccgaaaga cttcagcgcg cgcatcgcag
     3061 gcaaagaccc cgcgcaaagc atccgcatca aacctgaaga cgatttggca ggcatcaaaa
     3121 gccgcgccaa atacaccgag gtttatttgg taaaaggcga agacggcaaa atcgggcaaa
     3181 tcatcctgcc gatgcacggc aacggtttgt ggtcggtgat gtacggcttc gtcgccatcc
     3241 gacccgacgg caacaccatc aacggcatta cctactacga acaaggcgaa accccgggct
     3301 tgggcggcga aatcggcaat cctttgtggc agcaaaaatt cgtcggcaaa aaattgtttg
     3361 acggacaagg caaactcgcc ctgcacgtgg gcaaaggcgc gggttcggac aaagaacacg
     3421 gcgtagacgc cctctccggc gcgtcgctga cttccaaagg cgtgcaaggt tcgttcgcct
     3481 actggttcgg cgaaaacggc tatatcccct acctgaacaa attgaaatca gcaggagtgc
     3541 aataatggct gatatgaaac gcttgaaaca tttgatgttc tcacccttta tcgacaacaa
     3601 cccgattgcc ttgcaggttt tgggtatttg ttcggctttg gcggttacca ccaaacttca
     3661 gacggccatc gtaatgggta tttccgtagc tttggtaacc ggtttttcca gcttcttcat
     3721 ttcgctggtg cgcaactaca tccccaacag catccgcatc atcgtgcaaa tggcgattat
     3781 cgcgtcgctg gttacgctgg tcgaccaact gcttcaggca tttgcctacg aattgtccaa
     3841 gcagctttcc gtattcgtcg gcctgattat taccaactgt atagtgatgg gccgtgccga
     3901 agcatttgcg atgaaagagc cgccgctgga aagcctgatc gacggcatcg gcaacggtgc
     3961 gggctacggg atgatgctgc ttgtcgtcgc caccgtccgc gaactgatcg gctcaggcaa
     4021 acttttaggt tataccgtct ttcaaaccgt acaggacggc ggctggtatc agaccaacgg
     4081 cctcttccta ctcgccccca gcgcgttctt catcatcggc tttttgattt ggggactgcg
     4141 tacttggaaa cccgaacagg cggaggaata agctatggaa cactatttga gcctcttcat
     4201 caaatccgtc ttcattgaaa atatggcgct atccttcttt ttgggtatgt gtacgttttt
     4261 ggcggtatcc aaaaaagtat ccaccgcatt cggtttgggt gtggcggtaa ttttcgtact
     4321 cgggctgtcc gtccccgcca accaattggt ttactcgctg ctcaaagacg gcgcgattgt
     4381 cgaaggcgtg gatttgacct ttttgaaatt catcaccttt atcggcgtga ttgccgcttt
     4441 ggtgcagatt ttggaaatgt tcttggacaa attcgtcccc gccctctata acgcgctggg
     4501 catctacctg cctctgatta ccgtaaactg cgcgattttc ggcgccgtat cgtttatggc
     4561 gcaacgcgaa tacgacttcg gcgagtccgt cgtgtacggc ttcggcgcgg gtttgggctg
     4621 gatgttggcg attgtcgctt tggcgggcat taccgaaaaa atgaaatatt cggacgctcc
     4681 caaaggcctc aaaggcttgg gcattacctt tatcgccgcc ggcctgatgg caatggcgtt
     4741 tatgtcgttc tccggcatcc agttataaga aagggaccgg tatggagatt attttaggta
     4801 tcgtgatgtt taccgtcatc gtcttagctt tggcactgat gattctgttt gccaaatcca
     4861 agttggtcag cgaaggcgac atcaccatca aagtcaatga tgaaaaagaa ctgactatgc
     4921 ccgccggcgg caaactcttg ggcgcgcttg ccagccaagg catctttgtc ccctccgcct
     4981 gcggtggcgg cggttcgtgc ggacaatgcc gcgttgtcgt gaaaagcggc ggcggcgaca
     5041 ttctgccgac cgagctgtcc cacatcagca aacgcgaagc acgcgaaggc tgccgtttgt
     5101 cctgccaagt caatgtcaaa accgacatgg acatcgaagt acccgaagaa gtgttcggcg
     5161 tgaaaaaatg ggaatgcacc gtcatttcca acgacaacaa agcaacgttc attaaagaac
     5221 tcaagcttgc cattcccgaa ggcgaagaag ttcctttccg cgccggcggc tacattcaaa
     5281 tcgaagcccc gccgcacacc gttgcctaca aagacttcga cattcccaag gaatatcacg
     5341 aagactggga caaatacaat ctgtggcaat acgtttccaa agtggacgag ccgattttgc
     5401 gcgcctactc tatggcttcg tatcctgaag aaaaaggcat cattatgctg aacgtgcgta
     5461 tcgccacgcc tcccccgcgc gtacccgatg cgcctccggg acaaatgtcg tcctacatct
     5521 ggtcgctcaa acccggcgac aaagttacga tttccggccc gttcggcgaa tttttcgcca
     5581 aagacaccga tgccgaaatg gtatttatcg gcggcggtgc gggtatggcc ccgatgcgtt
     5641 cccacatttt cgaccagttg aaacgtttgc actccaaacg gaaaatcacc ttctggtacg
     5701 gcgcacgctc caaacgcgaa atgttctatg tcgaagactt cgaccaactc gcggcagaat
     5761 tcccgaactt cacttggcac gtcgccctgt ccgacccatt gcctgaagac aactgggacg
     5821 gctacacggg cttcatccac aacgtggttt acgaaaacca cctgaaaaac cacgaagcac
     5881 cggaagactg cgaattttat atgtgcggcc cgccgattat gaaccagtcc gtcatcaaaa
     5941 tgctcaaaga cttgggcgtg gaagacgaaa acatcctctt ggacgatttc ggcggttaaa
     6001 tattcggctt caaacacaaa aggaagaaac cccggtttct tcctttttta tccgatgccg
     6061 tccgaacacc cttcagacgg catccgccca tacccgtatc aatatcgttt ttttaccctg
     6121 cggcggatgt acggttaaat attttgacga aaaaacaaag aagtatactt ctttttagtt
     6181 atggttgatt ccataactaa aaaagtatcg atcaacccat tttgcgaggt acggataatg
     6241 tctgacagct ttgaaaacag ccccgaatac gatgattgga ttgaatcggg cggtcgtgat
     6301 gaagattacg aatactacta caacaaatgg caacgcagga cgcgctgacc attaaaccct
     6361 gacaaaccgg tttgtaaaac accggtttgt tttttattac cgccacccaa acgacgggcg
     6421 atgccgcccg aacgcccttc aaacggcatc ggcatagaaa cataccggcc gttttgcgat
     6481 ataattcaag ccatttgcca aaaccgtcaa acactatgcc gtctgaaaca cgcctgccga
     6541 accttatccg cgccttgata tttgccctgg gtttcatctt cctgaacgcc tgttcggaac
     6601 aaaccgcgca aaccgttacc ctgcaaggcg aaacgatggg tacgacctat accgtcaaat
     6661 acctttcaaa taatcgggac aaactcccct cccctgccaa aatacaaaag cgcattgatg
     6721 atgcgcttaa agaagtcaac cggcagatgt ccacctacca gaccgattcc gaaatcagcc
     6781 ggttcaacca acacacagcc ggcaagcccc tccgcatttc aagcgatttc gcacacgtta
     6841 ccgccgaagc cgtccgcctg aaccgcctga ctcacggcgc actggacgta accgtcggcc
     6901 ctttggtcaa cctttggggg ttcggccccg acaaatccgt tacccgtgaa ccgtcgccgg
     6961 aacaaatcaa acaggcggca tcttatacgg gcatagacaa aatcattttg caacaaggca
     7021 aagattacgc ttccttgagc aaaacccacc ccaaagccta tttggattta tcttcgattg
     7081 ccaaaggctt cggcgttgat aaagttgcgg gcgaactgga aaaatacggc attcaaaatt
     7141 atctggtcga aatcggcggc gagttgcacg gcaaaggcaa aaatgcgcac ggcgaaccgt
     7201 ggcgcatcgg tatagagcaa cccaatatca tccaaggcgg caatacgcag attatcgtcc
     7261 cgctgaacaa ccgttcgctt gccacttccg gcgattaccg tattttccac gtcgataaaa
     7321 acggcaaacg cctctcccac atcatcaatc ccaacaacaa acgacccatc agccacaacc
     7381 tcgcctccat cagcgtggtc tcagacagtg caatgacggc ggacggttta tccacaggat
     7441 tatttgtttt aggccaaacc gaagccttaa ggctggcaga acaagaaaaa ctcgctgttt
     7501 tcctaattgt ccgggataag gacggctacc gcaccgccat gtcttccgaa tttgccaagc
     7561 tgctccgcta aacatacaaa ccgattagga aacaccatga aaaccctgct cctcaccttc
     7621 ggcatcttcc tgaccgtcat catcggtatg gcggtcggct atattttctc caaacgcacc
     7681 atcaaaggca gttgcggcgg cattaccgct ttaggtatga aaaaaatgtg cgactgcgac
     7741 acaccttgcg acaccctgca aaaaaagctg gatgaagaaa aacaggcagg cggcatacgg
     7801 gttgaccgtt aacgccgaaa caaaatgccg tctgaagccc ggccggcggg ttcagacggc
     7861 attgccgctt tacttcgatc ggtttatacg caagacgcgc ttcaggaaat aaatcagatt
     7921 attccccaga cgccttcagg cttccttccg tgccaccgta accatagccg ggcgcaacac
     7981 acggtcggac agggtataac ccttcttcat cacaccaacc acggtattgg gttcctgctc
     8041 actggccacc gcctgcatcg cctgatggat attcggatcg agcttatcgc ccgctttagg
     8101 attgatttcc ttgatttgcg tggcatcaaa cgccttctgc aactcattca aagtcatctg
     8161 cacacccatt ttcagcgcat cgaaattacc gctctgatcc aaaagcgcca tttccagata
     8221 atccttgacc ggcaacattt ccacggcaaa cttctgtccg gcgaacttgt gcgtatcggc
     8281 aatttcctgc tggtggcggc ggcgcaggtt ttgctcgttt gccaaagcgc gcagttgttc
     8341 gtctttcaac tgcgcttcca actcggcaat ccgcgcctgc aaatcctcat aagccggctc
     8401 ggcggcagcc tgttcctgta caccgtccgc atttcctact gtctcgacgg tttccaccgc
     8461 ctccacattt tcaaccgctt cttcactgtt ttgctgctgt gtctgttcgc tcatatcgta
     8521 tccctcaaaa caaattggaa atcaaaatcc ggttattacc cgagcaagat aaggacattt
     8581 tcaagaaact tcaagcaaag gcaggaaatt tcacatccgc cgccgaatac cctaccgcaa
     8641 aaaccatata aacggtaaga taccgccgac cttatgcttt tcagacggca tccattatga
     8701 aaaaaaacca tttgaacaca accggtttcg acctctggca caccatccgc gaagaaaccg
     8761 cggccgctgc cgccgccgaa ccgatgctgg caagtttttt gcaccaaacc gtgttgcgcc
     8821 acgagtccct cggctccgtc cttgcctacc acctttccag caaactcggc agcccgatta
     8881 tggacgtgcg cgcgctgttt gaaatttacc agcaggcatt gggcagcgac acccaaatca
     8941 gcaaatgtgt cgaagcagac ttaaaagcca tctacgaacg cgatcccgcc tgcgacgaat
     9001 attcgctgcc gcttttatat ttcaaaggct tccacgcgat tcaggcacac cgcatcaacc
     9061 accggctgta tctcgacgga cgcaaaacgc tggcgtattt cctacaaaac cgtatgtccg
     9121 aagtattcgg cgtggacatc catcccgccg cccgtttagg atacgggctg atgctcgacc
     9181 acgccaccgg ctttgttgcc ggagaaaccg ccgtgttggg caacaatatt tcgattttgc
     9241 acggcgtaac gctcggcggt tcgggcaaag aaggcggcga ccgccacccc aaaatcggcg
     9301 acggcgtgat gatcggcgca aacgcctcga tattgggcaa tatccgcatc ggcagcaatg
     9361 ccaaaatcgg cgcgggcagc gtcgtggttt cagacgtgcc gccgtccatc acggttgtcg
     9421 gcgtacccgc caaacccgtg gcgcgatcgc tcaaaacccc gtcggcggat atggatcaaa
     9481 atatccagtt tgccgaaatc gactttatga tttgaatatc cgcaccaatg ccgtctgaaa
     9541 cgccaaacgg gcttcagacg gcattgcccg cttcaagcct gcgccttaat atgccgccga
     9601 tgccggcact tatcagtcgg acaaaatgcc ttttattcga tacgcccgat tcccacgcaa
     9661 ccggaaataa ccggtttcct tgtaaacggg cggagtcggg cgatgattcg gacaaagcct
     9721 atttcaaaag caaaatcagc agcaaaacaa ccgctatcgc tgccagccac aggctttgcc
     9781 gctgctgcac tttgaccaaa tggatatagg catcgcgcat ttcctgccgg cgcgcctcat
     9841 cgaccaaggc attgattttg cgcggcaggg aagggatgat ttgcgcccag tcgggggctt
     9901 cgtttttgag gttgcgccaa agggctttgg gaccgacctg cccgttcatc catttcacca
     9961 aaaacggttt ggcggttttc cacaagtcca aatcgggatc gagttggcgg cccaagcctt
    10021 cgatgttgag cagcgttttt tgcagcaata ccagctgcgg ctggatttcg acattgaagc
    10081 ggcggctgac ttcaaacagg cgcatcagta ccaagccgaa ggaaatctgc gaaatcggtt
    10141 tgttgaacac aggctcgcac acggcgcgaa ccgccgcttc caactcttcc gcacgcgtgt
    10201 cggcaggcac ccagcccgat tcgacgtggg cggtggcgac acgtcggtaa tcgcggttga
    10261 aaaaggcaag gaaattgatg gcgagatagc gtttgtcgta atcggtcagc gtaccgacga
    10321 tgccgaaatc aagggcgata tagcggttgt cggcggcaac caaaatattg ccggggtgca
    10381 tatccgcgtg gaaaaagccg tcgcggaaaa cttgcgtgaa aaagatttcc acgccgtaat
    10441 cggcaagttt gtgcaaatcg atgccgtctg ctttgagttt ggcgatgtcg gaaaccggcg
    10501 tgccgtccat ccattcaatc gtcagcacgt cgcttgtgca gtagtcgtaa aacaccttgg
    10561 gcacaatcag catattgctg ttttggaaat tgcgcccgag ttgtcctgca ttggcggctt
    10621 cgcgcatcaa atccaattcg tcgtgcaggt atttgtcgaa ctccgccacc acttcgcgcg
    10681 gcttcagacg cttgccgtct gaaaacaagc gttcgaccca agccgcgccg aaacgcatca
    10741 gcgacaagtc ttgttcgata aggggcaaga ggttggggcg caaaactttg accgccactc
    10801 gttcgcccga atgcaggcgg gctttgtgta cttgggcgat ggacgcgctg gcgacgggct
    10861 cggtttcaaa ttccgcatac agcttttcga tgggctgacc cagcgatttt tcgatttgtt
    10921 cacgcgaaag ccgcgcgtca aaaggcggca ctttgtcttg cagctttgcc agttcgacgg
    10981 cgtaatcgtg cggaatcaaa tcggggcgtg tggacaaaac ctgcccgaac ttgatgaaaa
    11041 tcggccccag gctttccaag gcaaggcgca gacggacggc aggcggttcg tttttcaatt
    11101 tggacgactg cggcatcatt ttcagcagag cgcatatcca accgctaccc atcagcgaaa
    11161 cgcacagacc tgccagccga tagcggtaaa aagtcccgac aatgaccgtc aggcgtttca
    11221 accatttcat atataaaatc ccgaatacag catttatcgg acaatcaaaa aatcttaccg
    11281 agcagatagc ccaaaaaggc aatcagcaac ccgaaaacca gcggtttgat attgcggggc
    11341 ggctcgggtt gcgaagatgc ggaaatatca tcttcccgcc cgccgtgaag ttcaagatat
    11401 tcgggcattt ccccatccgc gcctcctcct tcgccctccc cctcctccgg cggcacgacc
    11461 gtttccaact ccatatacgg gtaataatat ccaaacccaa atagctgaaa taatcatgca
    11521 actcttcccg tttcgcccaa gtgcattttt cctcaaccag cacttgcggt ttggaatcga
    11581 gcgattcgat caatccttcc gccttttctt cgccgagatt ctccttaagg cactcccgga
    11641 taagcccgcg atccacttgg ggcgggtagg aaacatatac atcgtacaaa tcgtttcggt
    11701 aatctttact catctcaatc ataagggaac aaatacgcct gataccgttc cgcccgttcc
    11761 tttcgggaaa cccgattcat cttcaagcaa tcggattata tgtaaattca tcccgataat
    11821 atattttcca aaagtaaaaa tgccgtccga acgttcagac ggcataacac aaaccagccg
    11881 gtcacacgga aaacgacgat ccgcaaccgc aggttgtttc cgcgttcgga ttgcggatga
    11941 cgaactgcga accctgcaga ctttccgtat agtcgatttc cgcaccgacc agatattgat
    12001 agctcatcgg atcgaccaaa aagaccaaac cgtttttctc aatttcaaaa tcgtcgtcgt
    12061 ttttgatttc gtcaaaagta aatccgtact ggaaacccga acagccgccg ccgttgacaa
    12121 aaacccgcaa tttcaaatcg ggattgtttt cttcggcaat caaatcggca actttggcac
    12181 agcagctgtc agtaaaaata atagggcttt cgtccgacat agtgttattc cttaatataa
    12241 tattttctgc attctctctc aacccccctt caaaggcaag cagggcttcg cctttgaaaa
    12301 aggaattgtc ccaatagata agtttgaccg cctgcaattt caagcctcaa actcaaaccg
    12361 tcctgccgaa ccactcgaca cgcccgataa tctccacatc atcggtcaag tcgttcaaat
    12421 tgatttcaaa agcaggataa gcctcgttgg cggaaatgac attgataatc ccgccgggga
    12481 cgatttgcag gcgtttgacc aacagatttt cattaatccg caacacatac agtccgtctc
    12541 tcggcgtatt ttccccatga ttgaccaaaa tcgaatctcc gtcattcaaa accccctcca
    12601 tcgaatcccc cttgacggaa attacagaca ggtttttcgt atctcgggta acataattct
    12661 caatccaatg ccgtctgaac gccattgtaa ataccggttc ctcatgaccg acaaactgcc
    12721 cgtatcccgc agccgcccga atatcatatc tcggcacaaa gacaaactcg tccgtatcaa
    12781 cttcatttcc caaggtatcg taagcaaggg atttttttgg ggcttcatcc ggaaacggat
    12841 taccctcccc tgtcagcagc caatcgatac tacacccctt caactgctta atttttttca
    12901 atgtttcaga cttcggcaga ccgccttcat tccatattct gctgaagccc gcaatcgtca
    12961 tttcaatatc ggatgcgatt tttgcctgcc tcgcttcact tttccataaa aaaaccagtc
    13021 tgtctttaaa ggtttccata aatatccttc cgctcccctt cattcaatca tctaaagtaa
    13081 acatgaaata tattttacat tagctatccc aaaaacggaa taactatgat aaactaaatt
    13141 taaattatga tttttcttat ccaatacata atttttcaga ttttccatag gtttaatccc
    13201 gaattgacag aaaaaggata gaaaaatgaa gaaaaccagc aaatatctta tctatactgc
    13261 ggcatttacc tcattctgct ttgccttcca agaaaaccgt tctgaagcca aacagcccga
    13321 catcacttta tccgcatccc tgtgcgaaca attcaacatg ctgaacgcca aagatatgga
    13381 tacagaacaa gtctcccttt ccaaagaatg cgacatcatc gagtcttcac acgactggga
    13441 aaaagagtac ggcaacttga acgaacagga aatgctcgcc ggcgtcgtct atgaataaac
    13501 ctgcctgcca tttgaaacat tatgcttgaa tgcattggag ccaaatgtat taaatcaaat
    13561 ataaaaccaa tatattcata aagttatata cttatggcca tgctgtagct tgaaacagcc
    13621 cgccgcccgc cctatttaca gcccatcggg acaaaatgtt ccaacatatt tctcaaaata
    13681 agcaaaatca aataggagta tccacatggc aaaagtaatc ggtatcgact taggtacaac
    13741 caactcttgt ttggccattt ccgaaaacgg tcaaaccaaa gtcatcgaaa acgcagaagg
    13801 cgcacgcacc acgccgtcca ttatcgctta tttggacggc ggcgaaatcc tcgtcggcgc
    13861 gcctgccaaa cgccaagcgg taaccaacgc caaaaacact atttacgccg ccaaacgttt
    13921 gatcggtcac aaatttgaag acaaagaagt ccaacgcgac atcgaatcta tgcctttcga
    13981 aatcatcaaa gccgacaacg gcgacgcatg ggtaaaagca caaggcaaag agctgtctcc
    14041 tcctcaaatt tccgcagaag tcctgcgtaa aatgaaagaa gccgccgaag cttacttggg
    14101 cgaaaaagta accgaagccg tgattaccgt ccctgcctac ttcaacgaca gccaacgtca
    14161 agccaccaaa gacgcaggcc gtatcgccgg tttggacgta aaacgcatca tcaacgagcc
    14221 gaccgcagcc gctttggcat tcggtatgga caaaggcgac aacaaagacc gcaaaatagc
    14281 cgtatatgac ttgggcggcg gtaccttcga tatttccatc atcgaaatcg ccaacctcga
    14341 cggcgacaaa caatttgaag tattggctac caacggcgat actttcttgg gcggtgaaga
    14401 cttcgaccaa cgcttgattg actacatcat tgacgagttt aaaaaagaac aaggcattga
    14461 tttgaaacaa gacgtaatgg ctttgcaacg tctgaaagaa gctgccgaaa aagccaaaat
    14521 cgaattgtcc agcggccagc aaaccgaaat caacctgccg tacattacca tggacgcaac
    14581 cggcccgaaa cacttggcaa tgaaaattac ccgcgccaaa ttcgagagcc tggttgaaga
    14641 cctgattgcc cgctctatcg agccttgccg caccgcattg aaagatgccg gcttgagcac
    14701 cggcgacatc gacgacgtga ttttggtcgg cggtcagtcc cgtatgccga aagtacaaga
    14761 agccgttaaa gacttcttcg gcaaagaacc gcgcaaagac gtgaaccctg acgaagccgt
    14821 tgccgtaggt gcagcgattc aaggcgaagt attgagcggc ggccgcagcg acgtattgct
    14881 gctggacgta acccctctgt ccttgggtat cgaaaccatg ggtggtgtga tgaccaagct
    14941 gattcaaaag aacactacta ttccgactaa agcgtctcaa gtgttctcta ctgccgaaga
    15001 caaccaaagc gcagtaacca tccacgtact gcaaggcgaa cgcgaacgcg cttctgccaa
    15061 caaatcttta ggtcaattca acttgggcga catcgcaccc gcaccacgcg gtatgccgca
    15121 aattgaagtt actttcgaca tcgacgccaa tggtatcttg cacgtttccg ccaaagacaa
    15181 aggcactggc aaagcggcca acattaccat ccaaggttct tcaggtttga gcgaagaaga
    15241 aatcgaacgc atggtgaaag atgccgaagc caatgccgag gaagataaaa agctgactga
    15301 attggtcgct tcccgcaacc aagccgaagc cctgattcac tccgtgaaaa aatctttggc
    15361 ggactacggc gacaaactcg acgctgccga gaaagaaaaa atcgaagccg cgctgaaaga
    15421 agccgaagaa gccgtgaaag gcgacgacaa aaccgccatc gatgccaaag ccgaagcact
    15481 gggcacagcc agccaaaaac tgggcgaaat ggtttacgcg caagcgcaag ccgaagccca
    15541 agccggcgag ggcgcacaag ccaatgcttc tgcaaagaaa gacgatgatg tcgtagatgc
    15601 cgactttgaa gaagtaaaag acgacaaaaa ataattgatg ccgtctgaaa aaaacgcgaa
    15661 ccattcggtt cgcgtttttt tcaattgaga taaaagacaa tagcatgaca gagattccaa
    15721 attcatctac cgtgatattc ccaagcccaa gttccaactg ttgcatcggt tatctggaaa
    15781 tttttcatat cattttctta tttacttaaa ttttttaata agataataaa taataattat
    15841 tatcatgagc taagaaatga aactaaatac tctcacatgg gctttgatga ccgttttttc
    15901 cgttgcgcca tcttgggcag aacaaccggc aaatactgaa gaaatacaac ccgtcaaaac
    15961 cttctccccg cccaaaccga ttgcaccgac cgccgcacaa ggctatttcc ccgaaaacca
    16021 attcgaccgc tccgaccgca gcgattatta ctttgttacc gaaaacatag accaagcctt
    16081 ccgtccgctg aaggcaaaca gcagttttta cggcaaaagc ttttacaact ccgttaccgc
    16141 gcaagcactc ggggcgaagg tatacggcgt agccaacctc aatcgcacca aggcaaacgg
    16201 ctacaaagat ggcggtgggc gcgacaccga ttggaaatac agccgcttca atcaggcttt
    16261 ggtactcggt ttcgtgccgt ctgaaaatca agaataccgc ctcacttatc tgcacgacga
    16321 catcaacaac gaccgccagc cgcaggtcgt caacgacgca ttggacaccg aacgccacat
    16381 ctccaaactc aacgtgcgtt ggggcaatgc cgatttgagc aatacggtca gcgcggaagc
    16441 aggcgtcatc aaactcaaac gccatgccga caattactcc ttgcgaccaa acaacacgcc
    16501 gcagcaagtg ttcgtagaac ttgaccgcaa agtgtatgat ttctcgctca aacacgatgc
    16561 ggatttcggc aaattccaca acacggcagc cgtcagctac cgcaacgaca gccaaaacgg
    16621 cgaacgcaac acccacactg ccatgtgcga tttcctcaac ggctaccgtt tcgccgatgt
    16681 gcacatcgac cgctggtgca ttgccgatac cttatcttac aagtttgacg accgacacaa
    16741 actgggcttg ggcttgggct taagctacga actcaacgaa gcggacattc gcaaaaatac
    16801 ggcacagcct gaaaacccaa taaaacccgg tttccccttc gcctcatcac aacaaatctg
    16861 gaaaacccat tacggctacg acttcaacgg caaagtgcgc cgtcatgctt tgtcaggcga
    16921 actcaaatac gatttcacgc cgtctgaaac gcaaaaatac agcgtttccc tcgcgcattt
    16981 ggaacgcatc ggcgacaaca ccgaacgctt caactcgctt gctgccatcg tgcaaaaccg
    17041 tatgagcggc atgctgatga accaaaatcc agccgccgcc attgcaggca atccccctgc
    17101 tgaaaactga aaaacacaac cgcatcaggc tcaccgccga cagccgcaac gactactaca
    17161 acggctacat gaactcgctc gcaggcgcgg gctggaacgt gggcggcacg cttgtggcgg
    17221 acaaagtcaa agacctgatt atttttgacc gcgcacacgg acaaagcggc acagcttcca
    17281 aagacggcgg catcatcaca cgcaacgtgg acgcacgatt gtttaccgcg caggcctacg
    17341 cgcgttacaa cttcaatcca cattgggcgg caggcatcaa agccgcctac aactacggac
    17401 acaacgaaac cgacggcagg ccgccctatc aaatccgtcc gtttgaagcc gccgtccaag
    17461 ccgactacaa aaactacttt gcccacggca gctacaacat cggcgcggca acacgctttg
    17521 tcgccaaaca aacgcgcggt gattttgata tggcaagcgg tctgggcata gacaaacgcg
    17581 aagccgccaa aggctttacc gttgccgacg tgtacgcagg cgtaaacatc aaagacaaat
    17641 acggcttgcg cttgggcgtg aacaacgtat tcaacaaaaa atacgtcgaa tacatcagcg
    17701 gcgaccacgt cctcgcccta tcccccagcg tagtgtatgc accgggcagg acatattggt
    17761 tgagtttgca tgcggcattt taaaaccatg ccgtctgaaa aatacctgca ttggcttatt
    17821 aagaattagg agataaaaaa tggatatgaa aagacgcgat ttcttaaaaa tgaccgccgc
    17881 gctggcagcc gcaggcgttt cgccttctct gcttgctgcc ggtaaagagc aatttaccgt
    17941 gtacggcgca ccggcaatgc ccagcgttac cattgccgta acggcgttgc aaggtaaact
    18001 ggcgaaacag gcggacgtgt cgctgaaaat ctggcgttcg cccgaccaac tgcgcgcggg
    18061 cgtggcaagc gggcaattta aagtgatgat gagtccgagc aatgtcggcg taaacctgcg
    18121 caaccaaggg cagaaagtcg gcatggtgaa tattttgacc aacggcatca cgcagttggt
    18181 ctgcaaaggc agcgcgattg cctcgccgca ggatttggtc ggcaaaaaaa tcctcgtgcc
    18241 gtttaaaaac gatatgcccg acatcgtgct gcaagccttg ttgaaaaaac tgaaaatcga
    18301 cgcacacaaa gtcagcatta cctacgctgc cacaccgccc gaagcagtcg gactatttcc
    18361 aagtaaaggt taccatgccg tcatcctgcc cgaaccgatg gcaaccgcca gcctactgaa
    18421 aggcaaaacc ataggtataa acgtcgtgcg cggttttgat ttggtaaaag catgggggca
    18481 ggcgtttggc accaaaccgc tgattccgat ggcaggtatc atcgccaacg aagaatattt
    18541 ccacgcacac aaggcgcagt tcgacatctt ccatcaagat ttgaaaaacg cgctcaactg
    18601 gatattggcc aaccgccaaa acgcggcgaa aatcggcaaa aactacctac ccgcccccga
    18661 acccgcccta gtcatgggct tggacggcgc acggctgacg gtaagcaaag gcagcgaagt
    18721 gaaaaacgag attttgaagt tttacgaaat cctgatgcag ttcaacccgg aacttttggg
    18781 cggcaagctg ccggataacg ggttcttctt ggcttaaagg gaaagaggcc gtctgaaaag
    18841 ggtagggtgg ttgtgaatac ctgatttatc atttttcaga cggcctatta aggcattgtc
    18901 agcggcgtgg gtcggattct tgaatccgac atttcggcta aacggggctg tcggatacaa
    18961 gtatccgacc tactcttttc agacggccta tcagataata gttcgaacag tacacaaaga
    19021 gccgtcggat tgcctgaaat gttgggtcat gacccaacct acgcttactg gggaaacggg
    19081 atcgaagtgt gaggtatgtg gcggggttgt ttaataatga aattttataa ctatttgata
    19141 gggtggtaat gctatgaatg aagataaata tagggaaaat atgacatggg atgaaattga
    19201 aaatgtagta ggaactttag tttctgaaga aagaatgctt aaatccaata gctttttaag
    19261 tattaaaaat agtaagaata ggttggctta atcacaacca taacccaacg aaaatactaa
    19321 aattccaagc taaggctatc ctttccaaac catccccatc aaccaaacca tgattaaaac
    19381 cgacaaaatc cgcaaaccgc agcctgcgct gttttacatc atcgactacc tttggagcgg
    19441 ctttgccggt ctgagcgtgg cgatggtggt ggtggcgttg tgggcgtggg gcagtgccgt
    19501 gttcggcgag tttatgctgc ctgcgccggt agaggtgttt caaaaatctt tggatttatt
    19561 gaaacatttt caggaaaatg aaatcgggat ttcgctgtgg cggtcggtag tgggtatttc
    19621 ggttgctttg atagcaggat tggcggcggg gctggtggcg ggcagtttta agacggcgat
    19681 ggcgttgctt aagcctgtga ttacgatttt gttagcaatg ccgccgatta tttgggtggt
    19741 gatggcgttg ttttggttcg ggttcggcaa tccgagcgtg ttgtttacca tcattgtgtt
    19801 ggttgcgccg ctgacgtttg cgagtgcggc ggtcggaatg gcgagcgtga acaagcagca
    19861 tgaggagttg tttgacgctt ataaattagg ccgtctgaaa aaaatccgct atctgtatat
    19921 cccgcatctg acgggctatg tgatttccag cgtcggcgtg gcggtggcga tgggggtgaa
    19981 ggcggtgatc atggcggaac tcttaggcgc gagcaaaggc gtgggcgcgc ggattgcgga
    20041 cgcgagggca atgctgtaga cttcgacggt aatggcttat gtggtgttgg tcatggtatt
    20101 tgtgtcgctg tttgaatacc tgattaccaa gcctttgtaa attttgttta tgccgtggag
    20161 gagatgatgc tctgtcttga aaacgtgcgt tttgaaatcc tccgcgaccc catcgtgcgc
    20221 gatttcagtt tgaacctgca acagggcgaa gtgaaagcct tgttcgggcc gagcggctgt
    20281 ggcaagacga cggttttacg gctgattgcg ggcttggaaa cgccgaaatc gggcacgata
    20341 cgtaatactt tccacaaaac gggttttctg tttcaggaaa accgcctgcc ggaaaacctg
    20401 accgcgatgc agaatatcgc gatttttatg gacaacccag acgaaggcga aatcgtcgcg
    20461 ctggcggcga aagtcgggct gactgcgggc gatttgaaca aatatccgac cgaattgtcc
    20521 ggcggcatgg cgaaacgggt ggcgtttttg cgcctgctgc tgtgcggctg cgacctagcc
    20581 ttgctggacg aaccgtttgt cggtttggac cgcgatttgc gcgatatttt ggttgctatg
    20641 ctggtggaaa aaatcgagcg gcagggcatg gcgtgtatac tggtaacgca cgaccgcttc
    20701 gaagccgcgc gcctgagcca tgaaattatg ctgctttccg ctaagggcat gaacgtgcaa
    20761 aacgtgataa ccctgcccac gccgctgtcc gaacgcgatt cggcttttga agaagtcgtg
    20821 gtggcaaggg agtttcaggg gattcattat tatgagtgat gaggggattt gagtgtctga
    20881 caaaccttgc ggttcgttgc cctctcccta accctctccc acggggagag ggttagcaag
    20941 cctcaggctt acctctttag cgaaagatac gaatctgtta tccgaacgcg attcggtttt
    21001 tgaaaaagcc gtggtgccaa gggggcaggg gattcattat gaggtgcttt atgttttcga
    21061 ctgtgattac tgctgctgtt ttatatattg ctacagcagt agatttgttg gtaatactat
    21121 taatattttt tgctagagca aatactagaa aagaatatcg agatatttat atcggacaat
    21181 atttaggttc tgtaatttta atattagtta gtttatttct agcttttgtt ttgaattatg
    21241 ttccggaaaa atgggtgttg ggtttattag gtttaatacc gatttactta ggtattaaag
    21301 ttgctattta cgacgattgt gagggcgaaa aaagagctaa aaaagaattg gatgaaaaag
    21361 ggttgtcaaa attagtcggt attgttgctt tggttacagt tgctagttgt ggtgcagata
    21421 atattggact ttttgttcct tactttgtga ctttagatct tgtcgactta ttagttactc
    21481 ttcttgtatt tttaatattg atttttgttt tagtatatac agcacaaaga ttggctaata
    21541 tttcaggtgt tggtgaaatt gtagagaagt ttagtcgttg
//
LOCUS       DAXFWM010000034        21497 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000034 DAXFWM010000000
VERSION     DAXFWM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21497
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.034"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            155..400
                     /locus_tag="ACFI8B_001190"
     CDS             155..400
                     /locus_tag="ACFI8B_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3834422.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEGLHHDTGHIGWRAMSKGK"
     gene            403..792
                     /locus_tag="ACFI8B_001191"
     CDS             403..792
                     /locus_tag="ACFI8B_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834423.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFNGGIGDIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYHKFKRFFPFVELPLL"
     gene            complement(832..2289)
                     /locus_tag="ACFI8B_001192"
     CDS             complement(832..2289)
                     /locus_tag="ACFI8B_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC3834424.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(2304..3134)
                     /locus_tag="ACFI8B_001193"
     CDS             complement(2304..3134)
                     /locus_tag="ACFI8B_001193"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC3834425.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(3211..3600)
                     /locus_tag="ACFI8B_001194"
     CDS             complement(3211..3600)
                     /locus_tag="ACFI8B_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC3834426.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(3747..4274)
                     /gene="nspA"
                     /locus_tag="ACFI8B_001195"
     CDS             complement(3747..4274)
                     /gene="nspA"
                     /locus_tag="ACFI8B_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC3834427.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRSGELSAGVRVKF"
     gene            complement(4717..5892)
                     /gene="hemW"
                     /locus_tag="ACFI8B_001196"
     CDS             complement(4717..5892)
                     /gene="hemW"
                     /locus_tag="ACFI8B_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC3834428.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTVEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQNRPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(5949..8420)
                     /gene="ligA"
                     /locus_tag="ACFI8B_001197"
     CDS             complement(5949..8420)
                     /gene="ligA"
                     /locus_tag="ACFI8B_001197"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC3834429.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAALNQRQTENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSVARQQGGLIAEEHIQELAYFQALGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGTVVKVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(8554..9840)
                     /locus_tag="ACFI8B_001198"
     CDS             complement(8554..9840)
                     /locus_tag="ACFI8B_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC3834430.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(10034..10606)
                     /gene="ampD"
                     /locus_tag="ACFI8B_001199"
     CDS             complement(10034..10606)
                     /gene="ampD"
                     /locus_tag="ACFI8B_001199"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC3834431.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            10690..11685
                     /gene="mltG"
                     /locus_tag="ACFI8B_001200"
     CDS             10690..11685
                     /gene="mltG"
                     /locus_tag="ACFI8B_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC3834432.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            11745..12365
                     /gene="tmk"
                     /locus_tag="ACFI8B_001201"
     CDS             11745..12365
                     /gene="tmk"
                     /locus_tag="ACFI8B_001201"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC3834433.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            12587..13867
                     /locus_tag="ACFI8B_001202"
     CDS             12587..13867
                     /locus_tag="ACFI8B_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HFC3834434.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            14269..15300
                     /gene="lpxK"
                     /locus_tag="ACFI8B_001203"
     CDS             14269..15300
                     /gene="lpxK"
                     /locus_tag="ACFI8B_001203"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC3834435.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            15506..16039
                     /locus_tag="ACFI8B_001204"
     CDS             15506..16039
                     /locus_tag="ACFI8B_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC3834436.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            16024..16131
                     /locus_tag="ACFI8B_001205"
                     /pseudo
     CDS             16024..16131
                     /locus_tag="ACFI8B_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            16261..16443
                     /locus_tag="ACFI8B_001206"
     CDS             16261..16443
                     /locus_tag="ACFI8B_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC3834437.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            16440..17201
                     /gene="kdsB"
                     /locus_tag="ACFI8B_001207"
     CDS             16440..17201
                     /gene="kdsB"
                     /locus_tag="ACFI8B_001207"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC3834438.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETVVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            17224..17739
                     /locus_tag="ACFI8B_001208"
                     /pseudo
     CDS             17224..17739
                     /locus_tag="ACFI8B_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            17896..17988
                     /locus_tag="ACFI8B_001209"
     tRNA            17896..17988
                     /locus_tag="ACFI8B_001209"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17930..17932,aa:Ser,seq:gct)
     gene            18262..19047
                     /gene="trpA"
                     /locus_tag="ACFI8B_001210"
     CDS             18262..19047
                     /gene="trpA"
                     /locus_tag="ACFI8B_001210"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC3834439.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIGSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVADAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            19084..19956
                     /gene="accD"
                     /locus_tag="ACFI8B_001211"
     CDS             19084..19956
                     /gene="accD"
                     /locus_tag="ACFI8B_001211"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC3834440.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(20016..20627)
                     /locus_tag="ACFI8B_001212"
     CDS             complement(20016..20627)
                     /locus_tag="ACFI8B_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC3834441.1"
                     /translation="MRRAIFLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            20735..20959
                     /locus_tag="ACFI8B_001213"
     CDS             20735..20959
                     /locus_tag="ACFI8B_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC3834442.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKVLAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            20990..21373
                     /locus_tag="ACFI8B_001214"
                     /pseudo
     CDS             20990..21373
                     /locus_tag="ACFI8B_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21377..>21497)
                     /locus_tag="ACFI8B_001215"
     CDS             complement(21377..>21497)
                     /locus_tag="ACFI8B_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC3834443.1"
                     /translation="SPEPNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         4715 a         5228 c         6502 g         5052 t
ORIGIN      
        1 taaaatattg tatatatata gataagaaat atattatacc gactggaaaa aaattctctc
       61 gcgatgattt ttattagcta atttaagtaa tttaagcaag ttttttagat actgaaactg
      121 gtaattatgt ttatgtaagc ataaggagtt cagattgaag gattctacag ggcaaatgag
      181 gctggcaacc aaggatttgg cggaagccat taaacgagga gaagtacgta gttctgcttt
      241 tacaacaaag caactaaagg caatcgaaaa aggagaatct aaaataccgg attacacttg
      301 gcatcatcat caagacactg gaaggatgca attgattaat gaaggcttgc atcatgatac
      361 cggccatatc ggttggagag cgatgagtaa aggaaagtaa ctatgtggaa aatcataaaa
      421 gaggatagtg atgatttagg atttgcaatt aaatgcttat tctctcaatc tattgattta
      481 aatgaattca agttatggat tgaacaagta atacgcgata tgcccatcga ggacatccct
      541 ttttatattt ttgacttggc ggatttcaat gggggaattg gagacattga caatattgta
      601 ggttttgttt cgagttacag cctatcaaaa tcgaaaaaaa atgccttgac aggcattgcc
      661 ttcttaaggg ggatagatgt ctatgatccg cctatttcaa aagaaaaagc attaaaagcc
      721 ttaaaaaaac atcctgaaat ttatcataaa tttaaacggt tctttccttt tgtagagctt
      781 ccgcttcttt aaaggacaat atgccgtctg aaaagttttc agacggcatt tttatttctt
      841 ccagtaggcg ggggtgaaga ggatgaagac ggtgaagatt tccagcctgc ccaagagcat
      901 tgcggtaacg cagatccatt tctgcatcac gtccaaaccg gcgtaattgc cggcgggccc
      961 gacttcgccc agtccggggc cggcgttggt aatgcaggcg atgacggcgg taaaggcggt
     1021 ggtaaattcc ataccgctcg ccatcagcag gaagctgaag aggacgacgg tcataaagta
     1081 gatgaagatg aaggacataa cggtcagcgc gaggcggtcg ggtatggctt tgccgctgat
     1141 tttgacggtg cggacggctt tggggtgcag cagcaccatc atttcgcgca ggctgaattt
     1201 gaacaggacg agggcgcgta tggttttgat gccgccgccg gtcgagccgg agctggcgag
     1261 gatgttggcg aggaaaaaca tccacaggga aatcaggagc ggccattgtg cgaagtcggt
     1321 gttggacagc ccgtttgcca atccgatgga gacgaagttg aaggcggtgt agcgcaggga
     1381 ttcgccgaag ccggcgtaat ggccggtgtg ccacaggtac agggcggcgg caaggatgct
     1441 gccggagagc agcagcagca tcgtccggca ttcttcgtct ttccaatagg ttttgaggct
     1501 gcggctgttg agggcggcga aatggctggc aaaattgatg ccgccgaaaa tagtgaaaac
     1561 gatgatgact gcttcgatga ggggggagtt gtaataaccg atgctggcat cgtgggtgga
     1621 aaacccgccc agcgagaggg ttgccagcgc gtgacagacg gcatcgaacc aacccatccc
     1681 ggcaaaatgc aggcaggctg ccgcgaggat ggtgatcagg gtgtagccga accagagttt
     1741 tttcgccact tgggaaatgc gcggcgacat tttgctttct ttgtcaatgc cggggatttc
     1801 ggctttgaat aactgcgtgc cgcctacgcc gagcataggc aggatggcga cggcaaggac
     1861 gatgatgccc atcccgccca gccagttgag catatgccgc caaaagttga cggagggggc
     1921 gagcccgtcg acgtggggga tgacggtcgc gccggtggtg gtcagtcccg acatcgattc
     1981 aaaaaatgcg tcggtaaagc ccatattcgg gaaatacagg tacatcggca tcgcagccat
     2041 agcggcaaac gccagccaca acatcaggac gagggtgaag ccgtcgcgcg ggcgcagttc
     2101 gcgcctgaac cggagggtgg cgagccggac gatgcacgag ccggaaaggg taacggtcgc
     2161 ggtggtggcg aaggcggtgt acgcaccgtc cgaaaaggcg taggagaggg cggcgggtat
     2221 cagcaggata aaggaaaaca gcatacccag tcgggagagg acgtgggcga tgggcaggat
     2281 tttgtgcata gtggggcggt ccgttatttt gcgaagcttt tccagtctat gccgccggcg
     2341 gcttggactt gggtaatttc ttccgtttcg aggttgacgg cggtcagctg cccgccccac
     2401 agcgcgccgg tgtccagcga gatgacgttg tcggcattcg tgtagcccag cgaggaccag
     2461 tgtccgaaga tgatggtatg gtcgaggttt tgccgatcgg gggctttgaa ccacgggcgc
     2521 aggtaaggcg gcattttttt cactgtggat ttgtagtcga aatccagttc gtttttaaag
     2581 gtcagggcgc gcatccgcgt gaaggcgttg acgatgaagc gcaggcgggc atagccttcc
     2641 aaaccttcgt cccatgcggc cggtttgttg ccgtacattt tggagaagaa tttgacgtat
     2701 tttttgccgc gcagttcggc ttcggcttct ccggcgagcg attcggcttt ggctatgcgc
     2761 cattgcggca ggatgccggc gtgtatcatc acgcggccgc cctcgcgtat caaaagcggt
     2821 tgcgcgcgca gccagtcgag catttttccg ccgtcggggt gtttgagtat gggttcgatt
     2881 gtgtcgctgc gtttgagcgc gccttcgccg cagccgacgg cgagcaggta caggtcgtgg
     2941 ttgccgagga cgatttgcac gctgttttcg tgccggatgc agaattgcag cgtttcgagg
     3001 gatttcgggc cgcggttgac gatgtcgccc gtcagccaga gggtgtccgt gccgtggttg
     3061 aagccgattt tgccgagcag cgcggtcagt tcgtcgaaac agccttggat gtcgccgatt
     3121 gcgtaatgtg ccattgcggg tgttgtgaag tgggaaagtg ttgcggttcg gacggcacgg
     3181 ttggaaatct tatgccgtcc gaacgtggaa ttatgcgttc aaaacgagga cggcttccgc
     3241 ttcgacctgc acgcctttgg gcagcgaggc aacgccgacg gcggcgcggg cggggaacgg
     3301 ctcggtgata aattccgcca tcacttcgtt gaagacggca aaattgccca agtcggtcag
     3361 gtaggcgttg agtttgacga tgtcgtccag cgaaccgcct gccgcttcgg cgacggcctg
     3421 caggttttgg aacacttggc gcgcttcggt gtggaaatcg ccgttgccga ccacagtcat
     3481 cgtggcggga tcgagcggaa tctgaccgct catgtaaacg gtgccgccgg cgcgcacggc
     3541 ctggctgtat gcgccgatgg cggcgggggc tttgtcggta tggatgacgg ttttggacat
     3601 ttcggattcc tcaaaaaata gggcggcaga agccgcagcg ttcgggatta tcgtacaaaa
     3661 ccgccggctt gtgtagtttt aggcggtata tgtgccggaa acaaaaaacc gccgaaggct
     3721 cggcggtttg cggaataacg cgtatatcag aatttgacgc gcacgccggc ggacagttcg
     3781 ccggaacgga cgtttttgac attgttgact ttgccgatgt agttgtagcg gtagccggca
     3841 tccagatcga cattgggtgt aacggcatag cttacgcccg ccaatacgcc gaggccggcg
     3901 gaggttttgc tgaagctgtc gctgccgccc aagtgggcgg aagcgcggtt gaggctcaag
     3961 cgcgcgccga aatacggttt gacgggcgat tgggtgtcga agtcgtaaat gacggacgcg
     4021 ccgatgctgt aaagtttgaa atcggtggat ggggcttgtt tatagttttt gtagcgcgtg
     4081 taatcgacgg cgaagcggag gtcgttgatg cggtagcctg cggagatgcg cgggctgaag
     4141 cctttggcag aacctaaaga gcttgaggct ttggcgtgtg cggcatcggc ttggacgtaa
     4201 aagccggatg cgccttccgc cagtgcggcg gccgggagtg cgagggcaat cagtgcggca
     4261 agtgcttttt tcatattttg gttcctttat ggtcagggag aaaaattgtt aataatccgt
     4321 taaagaatcc tgccgtatta tactgaaatt ttctttttgc atcgtaatat tttcaatgcc
     4381 tcaagatacg cggcggcatc cggctgcttt gccgacggca aagccgttaa cccgcgcgtt
     4441 gcccttaaac ggcggcatca cgcggcggat gggtgaaact tgcaaacggt ttggaaaaaa
     4501 ccgcaaaggc ggggcggcgg catttgtcag attgttgcag gcgcaggcgt acggtttttt
     4561 gtgtgcgggg ttaccttagg cgtcggacat ttccggcggc ggctgtgccg tctgaaacgc
     4621 ccggcggggg atgcggctgc gtttcccatc gataggcata tttgccggcc gcgttcgggg
     4681 cgggttttac ccgggcggcc gccgatttgt ttgcgcttat aaaaaacact gcaacaaatc
     4741 gtttaaaaac aagcgtcctt tttcggtcgg gcggaatacg gtcgggtcgg tttccagcag
     4801 gcctttttgc cttgccgttt cgatttgcac catgattttg gcggcgggta cgcccgtgcg
     4861 ctcctgcaac atcgcggcgg gtacgccgtc ggtcaggcgc agggcgttca tcatgaattc
     4921 gaacggcaaa tcttcggcgg cgacggtttt gcgttcgacg gcttcgcccg gccggttttg
     4981 cattaaggcg aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga tgcggtcggg
     5041 ataggagatt ttgccgtgcg cgcccgcgcc tatgcccaaa taatcgccga actgccagta
     5101 gttcaaattg tggcggcact gcatggtggg tttcgcaaaa gccgatgttt cgtagtggat
     5161 aaaacccgcg ccttccagtg tgccgtgtac cgcgtcttcg atgtcgagcg ccgcttcgtc
     5221 ttgcggcaaa cctttcggcg gcgtatgacc gaacggcgtg ttcggctcca tcgtcagatg
     5281 atatgcgctg atgtgggttg cgtctgtggc gatggcggtt tgcacgtcgt ccaatgccgt
     5341 ctgaacggtt tggttcggca gggagtacat caagtcgata ttgactttat caaataattt
     5401 taaggcggta tcgatagcgg ttaaggcttc cttgccgttg tggacgcgcc ccagccttga
     5461 gagcatatcg tcgttgaagc tttgtacacc aatagaaagc cgcgtgatgc ctgcgtcttt
     5521 aaatccttga aacttctcga tttcaactgt acccggattg gcttccaacg taatttccgc
     5581 ttcgggctgc aagcgcaaca gcgaacgcac gccgcttaac aaacggtcaa tcgattccgc
     5641 ctgaaacagg ctgggcgtac cgccgccgaa aaatatcgtt tccaccggcc tgccccaaat
     5701 attgggcaat tcaagctgca agtcggtcag cagcgcgtcg atataggcgg cttcgggcaa
     5761 tccgtttttc aggctgtggg aattgaagtc gcaatacggg cattttttga tgcaccacgg
     5821 gatgtggata tagagcgaca ggggcggcag ggcggtgagt cgggtgcggt ttggaaagga
     5881 aatggtgtgc atggtgcggt tcggaaaagt ggacgatgcc gtctgaaggc ggttcggacg
     5941 gcatgggttc agcagagcag ggtaagcagt tcggcttcgc tgaggacgga aacgcccaag
     6001 gcattggctt tttccagctt gctgcccgcg gcttctccgg cgacgacgta gtcggttttt
     6061 ttggacacgc tgccggacac tttgccgcct gcggcttcga ttagggcttg ggcttggtcg
     6121 cgtttgaagg tgggcagggt gccggttaac acaaaggttt tgcctgccac ggctttattg
     6181 atgccgtctg aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa gaaggttttt
     6241 aggttttcga gcagggcggt gttttgcgct ttgctgcgcc acgcctgcca gtcggcaggg
     6301 agggctttgt cgttttgcag tccttctata ctttgtccgg cgagttccca taaggcttgg
     6361 gctttgtttt cgctgatttt gaaaccgggc aggcgggtaa tccagcgttg cggcccggca
     6421 tattgtgcgg caggcaggct gacggcttgg gcttgcgggg caacgcctgc ggcgagcagc
     6481 tcgtctatca tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga acgcgccact
     6541 accgtgccga tgtcgggcag gcaggcgagg acgggttcgg gggcgcggcg gacgcgttcc
     6601 aatgtgccga atgcctgcgc cagcgttttg gcggtgcgtt cgccgacgtg gcggatgccg
     6661 agcgcgaaca ggaagcgggc aagttcgggc gttttgccgg attctatgcc tgcgaggatg
     6721 ttttgcgccc acttggtcgg ttgttttttg ccgttttggg tattgtattt agacaggtcg
     6781 cctgaaaccg tttctgcgtc gccgttttcg ttttcagatg accctttatc cgccgtttcc
     6841 ttcatttttt gcagggtcgg aatatcgatg cggtagaggt cggcgaaatg gcggacgagg
     6901 tcttgggcga ccagctgttc gatttgtttt tcacccaagc cgtcgatgtc catcgctttg
     6961 cgcgaggcga agtggattaa gccttgcgcg cgttgtgcct gacaaagcat accgccgctg
     7021 catcgggcga cggcttcgcc ttcttcgcgt tcgatttcgc tgcggcagat ggggcagcgg
     7081 gccggcaggc ggtagggctt atggagcgga acggattcgg tttgcttggc ggacggtgtt
     7141 tcggcaaaca ggtcgtcctg ctgatgcccg atgccgtctg aaacggcaac ggcggtttcc
     7201 tgcatcgggc ggcgttcgaa aatcacgcgc acgacttcgg gaatcacgtc tccggcgcgg
     7261 cgcacgacga cggtatcgcc gacgcgcacg tctttgcgcg atacttcgtc ctgattgtgc
     7321 agggtggcgt tggtaacggt tacgccgccg acgaatacgg gttgcaggcg ggcaaccggc
     7381 gttaccgcgc ccgtcctgcc gatttgcacg tcaatcgctt cgacaatggt cagggcttct
     7441 tcggcaggga atttgtgggc aaccgcccag cgcggcgcgc gggagatgaa gccgagttcg
     7501 tgctgttgcg ccaagctgtt gactttgacg accgtgccgt cgatttcgta gggcagttcg
     7561 gggcgttttt gctgcatgtg ttcgtaaaac gccaatactt cgccgatatt tttgaaacag
     7621 ccgaaattgc cattgggcag actgaagccg agtgcttgga aataggcgag ttcttggatg
     7681 tgttcttccg caatcaaacc gccttgctgg cgggcgacgg agtaggggaa aaagtgcagt
     7741 ttgcgttgcg cggtgatgcg cgaatcgagt tggcgcaggc tgccggcggc ggcgttgcgc
     7801 ggattggcga aaggtttttg cccgttttcg gtctgacgtt ggtttaaagc ggcaaaatcg
     7861 gctttgagca tcagcacttc gccgcgtact tcgatgagtt cgggcacgtt ttcgccgtgc
     7921 agccgcaggg ggatgttgga tacggttttg acgtttcggg taacgtcttc gccggtcgta
     7981 ccgtcgccgc gcgttgccgc ctgcaccaat acgccgtcgc ggtagagcag gctgatggcg
     8041 aggccgtcga atttgggttc gataacgtat tcgggattgc cgccgtccaa gccgtcgcgc
     8101 acgcgttggt cgaaggcgta catttcggca tggtcgaaca cgccgttttc atcttgcggg
     8161 gaaaaggcgt tggtcagcga cagcatcggc acttcgtggc gcacttcggc aaagcctgcc
     8221 aaaggctcgc cgccgacgcg ctgggtcggg ctgtcgggca gcttgagctc gggatggttt
     8281 cgttccaacg cttcgagttc gcggaacagg cggtcgtatt cggcatcggg tatgctgggc
     8341 gcgtcgaggg tgtagtattc gtaggcgtag cggttgagga ggtcggtgag ttcgtgtatg
     8401 cgttgtgcgg tcgggttcat ggattcgtac aggttcagac ggcatggaca aatgccgtct
     8461 gaacggtaaa acgtgttgga aatggtcgga ttttacctga aaacaaaagg cggatgcacc
     8521 gttgccgatg tatccgcctt tgtgtttgcg gagttatgaa aacaggcgca gggcggtttt
     8581 gccgcccggt tcgataccga ctttgagcat ctcggactga cgcgccaata cataagtgcg
     8641 tacgtctttg agccattggg tcgaaacttc ttccattttg tcgttgacca gattcaggtt
     8701 caactgaccg gacaggcgta ccgccaaatc cataaacaaa tcgtcgaagg ttttttcgcc
     8761 tgccggagag tgcgggatgt cgagcagcat actgaagcct ttgtaggact ggttgtccaa
     8821 aagggcattg gtaaacggct cgttgttgag cgagcagatg gagaacatgg tcgagcccga
     8881 cgtgtcggta tagtggaacg cgccgtcgtc ttccaaaacg aaacccacgc ccgttacggc
     8941 ggaacgcagt tctacgccgc tgatgctggt cggcgaaacc aaatggatgg cgatggtctg
     9001 gtcgacgcgc gcgcagaatg cgtccagtgc ggaagccact tcgataaagg cggcaaggtc
     9061 ggtgtgcagc gtctgaccgc ccatgctttg tgcgaatgcg tccgcctggc ggttgaatgc
     9121 ggagagttct tcctgcgagg caagtccgtt gcggctgact gcctggatac ccacgataaa
     9181 tgcctgatag cggatgcccg ggatgggttc ggcaatctgg aaatggtcgt ccatggtgca
     9241 gccgacaatc tggtagcggc agcggttgga aaggcgcggc agtgcgtgca gttctttggc
     9301 ttcggtcagc gcgatatagg agatgaagtc gaagcgcacg tcaaaccagg gcagctcgac
     9361 cttcgacagc tctttgagcg taatcagcgg ttttgccggc gtttgcggaa cgggaacggg
     9421 ttttgccggc gcgtcggcag gtttcggtgc ggaatgtccg gtttggggtt cggaaacggt
     9481 gtgggcggag ttgccgataa tgccgatttc ttccaaggcg gtttcgattt cggttttgaa
     9541 cggggaggct tttgcctgtt tctgcttggc gatgtagacg gcatcctgtt cttgcaggtt
     9601 gcgcatggcg gagtcttggg gtttggccgg ttttttgacc gccggttggg gtttcggcat
     9661 catgactggc ccgccggacg gtttgccgtc gcggacatgg ctggttttgc tgttgagcag
     9721 ggcatctttg tcggagtgtc cgaactggtc gcgcactttt ttgcggtatt ggttttcctg
     9781 atacatattg taggcgacaa cggcgaggac ggcggcgagg aacagtacga tgtaaatcat
     9841 ggcaatcact ttaaatttcg ggatgcagga tgcgcaaagt gcgggtactg cggttaaatc
     9901 gggcttgcgt ttccggcagt ctgacggaac ggccggttat aacgtttgaa ttataacgaa
     9961 aattgcaggg tctgacagca gtgtgtcgaa ataagcggaa attttccgaa atgccgtctg
    10021 aaatctgtgg ttttcagacg gcatttctgt ccacgggaaa ccctttttcc cgtatccgcc
    10081 gccagtcgaa aaaatggccg gggtcggttt tgcggccggg tgcgatgtct tggtgtcccg
    10141 ttaccgccgt gatggggtag cggcggcaga gggctgccaa cagtgtttcg agcgaacggt
    10201 attgcgcttc ggcaaacggt tcgaaatcgc agccttccag ttcgatgccg atggaaaacg
    10261 cgttgcactt ctcccgcccc cgaaacgagg atgcgccggc gtggtacgcc atgtcgccgc
    10321 atgatacgaa ctgcaccgtt tcgccgtcgc gtgcaatcag gaaatggctg gacacgtgca
    10381 aagtgtgtat caggctgaag aacggatgtc cgtcggggtc gagccggttg gcaaacagct
    10441 tttccaccgc atccgtgccg tattcgaacg gcggcagcga aatgttgtgc agcacgatca
    10501 gggaaaccgt ttctcccgtt tccctcgggc tgaaattggg cgacggggta tggcgtatgc
    10561 tttgaagcca gccgttttgc cagtgtgctc cggcgtgatt gtccatgatg ttcttcctgt
    10621 ccggcgggca atttgggtta tactgtcgcc cgaattttaa gacgtattcc gaatgctggg
    10681 aatcctacca tgttgaaaaa aatgttgaaa tggactgccg tttttttgac cgtatcggca
    10741 gccgttttcg ccgcgctgct ttttgtccct aaagacaacg gcagggcata ccgaatcaag
    10801 attgccaaaa atcagggtat ttcgtcggtc ggcaggaaac ttgccgaaga ccgcatcgtg
    10861 ttcagcaggc atgttttgac ggcggcggcc tacgttttgg gtgtgcacaa caggctgcat
    10921 acggggacgt acagattgcc ttcggaagtg tctgcttggg atatcttgca gaaaatgcgc
    10981 ggcggcaggc cggattccgt taccgtgcag attatcgaag gttcgcgttt ttcgcatatg
    11041 aggaaagtca tcgacgcaac gcccgacatc ggacacgaca ccaaaggctg gagcaatgaa
    11101 aaactgatgg cggaagttgc gcccgatgcc ttcagcggca atcctgaagg gcagtttttt
    11161 cccgacagct acgaaatcga tgcgggcggc agcgatttgc agatttacca aaccgcctac
    11221 aaggcgatgc aacgccgcct gaacgaggca tgggcaggca ggcaggacgg gctgccttat
    11281 aaaaaccctt atgaaatgct gattatggcg agcctgatcg aaaaggaaac ggggcatgag
    11341 gccgaccgcg accatgtcgc ttccgtcttc gtcaaccgcc tgaaaatcgg tatgcgcctg
    11401 caaaccgacc cgtccgtgat ttacggcatg ggtgcggcat acaagggcaa aatccgtaaa
    11461 gccgacctgc gccgcgacac gccgtacaac acctatacgc gcggcggcct gccgccaacc
    11521 ccgattgcgc tgcccggcaa ggcggcactc gatgccgccg cccacccgtc cggcgaaaaa
    11581 tacctgtatt tcgtgtccaa aatggacggc acgggcttga gccagttcag ccatgatttg
    11641 accgaacaca acgccgccgt ccgcaaatat attttgaaaa aataaaccat gccgtctgaa
    11701 aagtttgtgt tttcagacgg cataccctta ccggaactgc aagcatgaaa ccgcaattca
    11761 tcactttgga cggcatagac ggtgccggaa aatccaccaa ccttgccgtc atcaaggcat
    11821 ggtttgaacg gagggggctg cccgtgctgt tcacgcgcga gccgggcgga acgccggtcg
    11881 gtgaggcctt gcgcgaaatc ctgctcaacc ctgaaaccaa agccggtttg cgtgcggaaa
    11941 cactgatgat gttcgccgcg cgtatgcagc acatcgagga agtcatcctg cccgcgcttt
    12001 cagacggcat ccatgtcgtg tccgaccgtt ttaccgatgc gaccttcgcc tatcagggcg
    12061 gcgggcgggg gatgccgtct gaagacattg aaattttgga acattgggtg cagggcggtt
    12121 tgcgccccga tttgaccctg ctgttggatg tgccgctgga agtatcgatg gcgcgtatcg
    12181 gacaggcgcg cgagaaagac cggttcgagc aggagcaggc ggatttcttt atgcgtgtgc
    12241 gcggcgttta tctcgaccga gccgccgcct gtcccgaacg gtacgccgtt atcgacagta
    12301 accgcagctt ggatgaagtc agaaacagca tagaaaaagt gttggacgga catttcggct
    12361 gttgatgcgg caaatattga aacaagcgca tccgcccgcg ccgaaaacca aacggcagtg
    12421 ccgcaggtga aaatggcggt atgcgccaaa ctttcggcat gatagaatta cgctcggtta
    12481 caaggcagga tgcgtcggca atattaacga accgcccgta acatgatgac ccgaaagcgt
    12541 ttcggacagt tcgattcaaa tctttttctc gcaacaggat tgacacatgg aaaactcatt
    12601 gaaagaagcc gccctcaagt tccacgaatt acccgttccg ggcaaaattt ccgttacccc
    12661 gaccaaatct ctggcgaccg acaaagattt ggcgttggcg tactctccgg gcgtagccgc
    12721 cccctgtatg gaaatccatg ccgatccgca aaatgcctac aaatacaccg gcaaaggcaa
    12781 cttggtcgcc gtcatttcca acggtacggc cgttttgggc ttgggcgaca tcggcgcgct
    12841 ggcgggcaaa cccgtgatgg aaggcaaagg cgtattgttc aaaaaattcg ccggtgtgga
    12901 cgtgttcgac atcgaaatcg acgaaaaaga cccgcaaaaa ctggtggaca tcatcgccgc
    12961 tttggagccg actttcggcg gcatcaacct cgaagacatc aaagcacccg agtgttttta
    13021 catcgaacgc gaattacgca aacgctgcaa aatccccgta tttcatgatg accagcacgg
    13081 cacggccatc attaccgccg ccgccgtatt gaacgccctg cgttataccg gccgtaaaat
    13141 cgaagaagcg actttggtgt gctccggcgc aggtgccgcc gcgattgcct gcctgaacca
    13201 actgctggat ttgggcttga aacgtgaaaa cgtaaccgtt tgcgactcca aaggcgtgat
    13261 ttaccaaacc cgcgaagaca aagaccgcat ggacgaatcc aaaaaattct acgccgttga
    13321 agacaacggc cggcgcgtac ttgccgatgc ggttaaaggc aaagacatct tcttgggcct
    13381 ctccggcgca aacctgctga cgcctgaaat gttgaacacg atgaacgaaa aacccatcgt
    13441 gttcgccatg gccaacccga atccggaaat cctgccgccg ctggcgaaag aaacccgtcc
    13501 ggacgtggtt atcggtaccg gccgctccga cttcccgaac caagtgaaca acgtattgtg
    13561 cttcccgttc atcttccggg gtgcgttgga tgtcggcgca accaccatca acgaagaaat
    13621 gaaacgcgcc tgcgtgtatg ctttggcaga tttggcgatg gaagaagtaa ccgaagaagt
    13681 ggttgccgct tacggtaaga aattcgaatt cggcgcggaa tacctgattc ctactccgtt
    13741 cgattcccgc ctgctgcctc gcgttgctac ggctgccgcc aaagcagcga tggaaagcgg
    13801 cgtggcaacc cgtccgattg cagatttgga agcttacgct gccaagttgg gcgaatggaa
    13861 gctgtaagcc gtttgtggtt aaaatgccgt ctgaactgtt ttcaggcggc attttgttgt
    13921 cagattgata aatgaaagat actggaaaat gaaagagatg aaacctgtcc gttatcatat
    13981 tggcgatata cccgaaactt caaaacaaac cgcccccggt catgacgaca gggcagtggg
    14041 tgttgacgat gattgttttc atgattcctt tggtcaatat cgttatgttt tttgtttggg
    14101 cgttcggcag aggcaacccc aaccgtgcca atttctgtaa agcactgttc ttatttacct
    14161 tgttggttcg cttatcggtt tgattttcat attgcttata ggtgggtctg catcgggtac
    14221 gcattattaa tgtgccggct gattctgctt cgaagatttg tatcgaatat gccaaatttt
    14281 tttaaatttc ataccgttat cgaacggcat tggcaaaaac cttatccggt tttgtctttt
    14341 ctgctcaagc cgctttccgg gctgtttgcc aaaattgcgg caaaatggcg ggcggatttt
    14401 ttatcgggaa aacggcaaag cgaaaagctg tccgtgcctg tggtcgtggt cggcaacatc
    14461 cacgcgggcg ggacgggcaa aacgccgatt gccgccgcgc tggtgtcggg cttgcaggaa
    14521 aaaggtgtca aggtcggcat catcagccga ggctacgggc gcaagagcaa ggcggtttat
    14581 gtattgaatg ccgcgagccg tgcggaagat gcgggcgacg agcctttgct gctgttccgt
    14641 aaaacaggtg cgccgacggc agtgggcagc agccgtgtgg aggcaggcag ggcgttgctg
    14701 gcagcgcatc cggagcttga attgattgtg gcggacgacg gtttgcagca ttacgccctg
    14761 caacgcgatg tggaaatcgc ggtgtttccg gcggcggata cggggcgtac ggatttggac
    14821 ttgctgccca acggcaattt gcgcgaacct ttgtcgagat tggaaagcgt ggatgcggtg
    14881 gtggtcggcg gcagggcggc ggatggtttt atgccgtctg aacatttgtt cggcagccgt
    14941 attgaggcgg gtgcggttta ccgtttgaac cgtccgtctg aaaaactgga tatttcgaca
    15001 ttgtcaggga agcgcgtcgc agcggttgcc ggtatcgcca gaccgcagcg tttcttcgat
    15061 actctgacac acatgggcat ccgccttgac caaacggttg ctctgcccga ccatgccgat
    15121 attttcaatc gggatttgcc gcctgccgat gtggtgctgg taactgaaaa agatgcggtc
    15181 aaattttcag acggcatttg caccgataat gtttgggtgc tgccggtttg tgcgataatc
    15241 gaacctgatt tggcggagtt tgtgctggag cggttggagg gtgtaccgaa ggccgtctga
    15301 aagcgcggtt tggacgaagt gataacggac tggaataaga acgccctacg ccgtcattcc
    15361 cgcgcaggcg ggaatctaag tctcgaattt tcaggaatgc ctaggagact tcagaaatcc
    15421 caaatctccg gatttccact tgggcaggaa tgagaaagcc ggttgtattt tttatctgca
    15481 ttaatcattc gttaaaggag tggatatgaa gctgaaaacc ttgttattgc ccttcgccgc
    15541 actggcattg tgtgccaacg catttgccgc cccgcccggc gacgcgtcgt tggcacgttg
    15601 gctggatacg cagaatttcg accgggatat agaaaaaaat atgattgaag gctttaatgc
    15661 cggatttaaa ccgtatgcgg acaaagccct tgccgaaatg ccggaagcga aaaaagatca
    15721 ggcggcagaa gcctttaatc gttatcgtga gaatgttttg aaagatttga ttacgcccga
    15781 agtgaaacag gctgtccgca ataccttatt gaagaatgcc cgtgaaatat acacgcaaga
    15841 agaaattgac ggcatgattg ccttttacgg ttcgcctgtc ggtcagtccg tcgttgccaa
    15901 aaatccgcgc ttaatcaaga aatcgatgag tgaaatagcg gtatcttgga ctgcattgtc
    15961 agggaaaatc gcgcgacatc atctgcccga gtttacggaa gagttacggc gcatcatctg
    16021 cggcggtata gtggattaaa tttaaatcag gacaaggcgg cgaagccgca gaccgtacaa
    16081 atagtacggc aaggcgaggc aacgccgtac cggtttaaat ttaatccact ataaaaatcc
    16141 cgatgcgggc tgcaagcagg ccggacagct tgggaaaagg tatcagaagt aaataatagc
    16201 cgcctgaaat attgcggagg gcatccgatt gattaaacca tcaaacccga aagcaacctt
    16261 atggaaaaaa aattcttaga catcctcgtc tgccccgtta ccaaaggcag gctggaatat
    16321 catcaggaca aacaggaatt gtggagccgt caggcgaagc ttgcctatcc gattaaagac
    16381 ggcattccct atatgctgga aaacgaagca cgagcgttga gcgaagagga actcaaagca
    16441 tgaccgaatt cgtcgtattg attccggcgc ggctggattc gtcgcgcctg cccggaaaag
    16501 ccttggcgga catccacggc aaaccgatgg tcgtgcgcgt tgccgaacag gcggcaaaaa
    16561 gcaaagccgc gcgcgtcgtc gtcgccaccg atcatcccga cattcagacg gcctgtcagg
    16621 cgcacggtat cgaagtcgtc atgacttcaa accggcacga aagcggcacg acgcgccttg
    16681 ccgaagccgc cgccgcgctg aagctgccgc cgcatttgat tgttgtgaac gtacagggcg
    16741 acgagccgct gattgccccc gaactcatcg accgcaccgc cgaagtactc gtcgaaaaca
    16801 acgtccaaat ggcgaccgcc ggccacgaat tgcacgattt cgacgaattg atgaatccca
    16861 atgccgtcaa agtcgtcctc gacaaaaacg gcaacgccat ctacttcagc cgtgccccga
    16921 ttccctatcc gcgcgatgcg atgcgtgccg gaaaacgcga aatgccgtct gaaaccgtcg
    16981 tcctacgcca tatcggcatt tacgcctacc gcgccggctt cctgcaacgc tatgccgaaa
    17041 tgagcgtctc gccgctggaa accatcgaat cgctggaaca actgcgcgtc ctgtggcacg
    17101 gctaccccat cgccgtcgaa accgctaaag aagcccccgc cgccggtgtg gatacgcagg
    17161 aagatttgga cagggttcgc gctgtatttc agaccgtata aaacaggttc aaagggaaaa
    17221 gatatgcagc aacatattga aaagtggcaa cacttgagcc gtgaagaaca gaaaatcctt
    17281 gccgaagtat ggggtctcgt gcaaaacgat gatcaggagg ttcactatga aatgctcaaa
    17341 ttgaacgcac ctgatgaagt cagcggtgaa ttttggttca gaatggcaga aacactcagc
    17401 accctgccgc ccaatcgttc cctcgacctt agaatgaacg gcggcaggct gtcgaccgcc
    17461 gtatccatcc tttccgtcat gattgaagac aatcccgaca taccgcagct ttgggcgtaa
    17521 aaaattaccg ccctcaatta tttggtacac ggacacaaag cccgtgccga cggtttggta
    17581 caacagcccg acaaagcggc agaagccaac gaagaggaat acctgaccaa agccctgtcg
    17641 caaaacctgc tgtcaacatt ggatgcctca cttgcacgtt ttcctgaaga cgcgtggttt
    17701 caggaaatca aacaggatgc acaaaagcat tttgcttgag gacgtggcgg tcaggaatat
    17761 ttccattcag gaagaaaaga agtgcctaat tgggtataat cagggtaaat cttattttat
    17821 ttcaaaagat taatatttgt tttccgtttt tccttgacgg tatcggaaaa gttgattata
    17881 gttacagctt ccttaggagt aatggctgag aggctgaagg cacttccctg ctaaggaagc
    17941 atgtgggatc aacctgcatc gagggttcga atccctctta ctccgccaga taaaaaaata
    18001 gacgctgtgt tttacagcgt ctattttttt atgcaatttt atagcgggtt ggcgcaaacc
    18061 cggtatggta ttgccctgtc ttgattctga attttgttaa tccgagatgt ttgccgttta
    18121 tttttgcctc gttcaaacgg cggctctgat ctgcgcggtt tctgtttgcc gtattcgcct
    18181 atccgtaccg caaatgttat actgggaaaa attcaccgat tgtattttac ggcgtatttg
    18241 ccgataggat ggaagagaca aatgagcaga atccggcagg cttttgccgc tttggatggc
    18301 ggaaaggcat tgattcccta tattgcggtg ggcgaccccg atattcggac aactttggca
    18361 ttgatgcacg gcatggttgc aagcggtgcg gatattttgg agttgggcgt gccgttttcc
    18421 gatccgatgg cggacgggcc ggttattcag cgtgcggcgg agcgggcgtt ggcaaacggg
    18481 atttcgctgc gcgatgtctt ggatgtcgtc agaaaattcc gtgaaaccga cacgcaaacg
    18541 ccggttgttt tgatgggata tttgaatccc atacataaga tgggttatcg ggagtttgct
    18601 caggaagccg caaaggcggg ggtggacggc gtgttgacgg tggattcccc catcgaaacc
    18661 atcggttctt tatatcgcga gctgaaggat aacgaggtcg actgtatttt cctgattgcg
    18721 ccgacgacga cggaagaccg tattaaaacc attgccgagc tggcaggcgg atttgtctat
    18781 tatgtttcgc tcaagggcgt aacgggcgcg gcaagtttgg atacggatga ggtttcgcgt
    18841 aaaatagagt atttgcgtca gtatatcgat attcccatcg gtgtcggttt cggcatcagc
    18901 aatgcggaaa gcgcgcgcaa aatcggccgg gttgccgacg cgatcattgt cggcagccgg
    18961 attgtgaaag agattgaaaa caatgcgggc aacgaggctg ccgccgtcgg tgctttggtc
    19021 aaagagttaa aggatgccgt gcgctgattc tgtcgcgcat tctaaatgtt ttaggagttg
    19081 tccatgagct ggttagataa aatcctgcca cccaaaatca agaatcgtgg gaaagacggt
    19141 tcttccaatg ttcccgaggg tttgtggcgc aaatgcccgt cttgttcggc aaccgtttat
    19201 tcgaccgagt tgcagcagaa caatcaggtc tgcccgaaat gcaaccacca caatccgtta
    19261 tcggcgcgcc agcgtttgaa cctgcttttg gatgaggaag gcagggagga agttgcgggt
    19321 aatgtcaaac cgaccgatcc tttgaagttt aaagacagca aaaaatatcc ggaccgtttg
    19381 agtgcggcac gcaagctgac cggggaagat gacgcgctgg tggtgatgaa ggggatgatg
    19441 aacggtctgc ccgtcgtcgt tgccgcgttt gaattccgct ttatcggcgg ttcgatgggt
    19501 tcggttgtgg gcgagcgttt cgtacagggt gtccgccgtg cggttgccga taattgtccg
    19561 tttgtctgtg tggcggcttc cggcggcgcg cgtatgcagg agggtgtaaa ctcgctgatg
    19621 cagatgacga aaaccagtgc cgcgctgcat ttgctgacgg aaaaacgcct gccgtttata
    19681 tcggtgttga ccgatccgac tatgggcggc gtatctgcca gcttcgcatt tttaggcgat
    19741 gtcgtgcttg ccgaaccgaa cgcgctgatc ggttttgccg gtccgcgcgt gattgagcag
    19801 acggtgcgcg aaacgctgcc ggaaggcttc caacgcgccg agtttctgct ggaaaaaggc
    19861 gcgatcgacc agattgtcga ccgccgcgat atgaagcggc gcatcagtga tttgattacg
    19921 ctgttgtgcc gtcaggacaa agtttccgcc acctgatggc tgatgaatcg aatgccgtct
    19981 gaaaccgatg tttcagacgg catttttgtg tctggttatt tgtcgtgcgg cttcatcgac
    20041 ggggcatagc gtccggcacg ttctttcagg cgttgtacca aaccttgcgt gtcggcgggt
    20101 acgccgccct cgcagaatgc ctgatacagg acggcgcgca atgcgtcgtt gcgcccaagt
    20161 gtgccgccta tcggtttcca ttcggcgttt cggggctgta tccagcggct gttgaccgta
    20221 tcgccgtatc cgaatacttt gtaggaggaa agtttgccgt tgccgaaccc gatggaggag
    20281 cgggcgcaga atatgccttc ggcagtcagg ttgtcgtagc ctttgtcgga gcggatattg
    20341 agaatgtagc ggatgctgcc gtcgggcgcg ggcataattt gcaggctgtc gagcaggatt
    20401 ttcggctgtt tgccgtaatt ttcatccaca taaatgtcaa accagccgtc cgagtgcgca
    20461 tcgggcagcg gcggcagttc ggcggtatgt tctttaaatt cgcgggcggc ggcttcttcg
    20521 ggcgtttcgc ggtagcgggt gttgatcggc gtgtcttttt ggctgaagcc ggcggcaagg
    20581 gacgtgccga cagtcagggt cagaatcaga aagatggcgc ggcgcataag tttctccaaa
    20641 ttgaaaacgg ctttatttta tggtttggcg ggaagggctg caagcaatcg gggtataatc
    20701 tgacctgatt ttcattttaa agcggtgtcg aaccatgaac agcgaaactt tagacgtaac
    20761 cggattgaaa tgtcccctgc cgattttgcg ggcaaaaaag gttttggcgc aaatgcggca
    20821 gggcgaggta ttgaccgttc tggcgaccga cggcggcgcg ccgggggact ttgaggcttt
    20881 ttgccgccaa accggtcatg tgctgttgga ttcttccgaa caggacggcg tgttcacact
    20941 ggtcgtcaag cacaaataaa tgccgtctga aatgcggatg tcctgattga tgttattgct
    21001 gtcggcattt gtgcttgctg cgtgtaatgc ccaaaaccgg caatccggtg tacggcatca
    21061 ggattttgca cgcgtagccg tcgatttgtt tcggagtgcc tgcgtcctga ctcaaggtag
    21121 tttcgaaccg gtttccaagt ttgccgctgt cggacatttt gtccctgtcg gaaaggaaga
    21181 cctgtcccgt ctgccgcccg ctgttgcaga acccgatgta caggcgttgt ggacgcttga
    21241 acgcgatggc ggcatttatt atttgagcct gacgcgcgac agttgcagtg tgaaagccga
    21301 gcgggcagac agtgccgcgc ttttggaaca tttctcaggg cttgtccgac agccgccgaa
    21361 aaatgccaat atatagtcaa ttaaaatcaa aataggacag tagtgcatcg tcaaatcggg
    21421 cgtaatcaga caaaacggtt cgcagatacc gcttaatatt cgcccacact ttctcaatcg
    21481 ggttgggctc gggcgaa
//
LOCUS       DAXFWM010000035        21356 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000035 DAXFWM010000000
VERSION     DAXFWM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.035"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..527
                     /locus_tag="ACFI8B_001216"
     CDS             <1..527
                     /locus_tag="ACFI8B_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3834444.1"
                     /translation="RRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKT
                     QMRTSFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGE
                     TVLLADPSLGHVSTSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQ
                     TRFTVEQIQMRQKR"
     gene            complement(793..1344)
                     /locus_tag="ACFI8B_001217"
     CDS             complement(793..1344)
                     /locus_tag="ACFI8B_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834445.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRAAKAGGAGKADG"
     gene            1582..3054
                     /gene="pyk"
                     /locus_tag="ACFI8B_001218"
     CDS             1582..3054
                     /gene="pyk"
                     /locus_tag="ACFI8B_001218"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC3834446.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            3288..4640
                     /locus_tag="ACFI8B_001219"
                     /pseudo
     CDS             3288..4640
                     /locus_tag="ACFI8B_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
     gene            complement(4723..5145)
                     /locus_tag="ACFI8B_001220"
     CDS             complement(4723..5145)
                     /locus_tag="ACFI8B_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834447.1"
                     /translation="MAGCLTDLENCLEKYLEQFGPVSESIEACTAKLQEQPSFFNRLM
                     KANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHNRKADYAEKGKERIAL
                     FPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(5323..5472)
                     /locus_tag="ACFI8B_001221"
     CDS             complement(5323..5472)
                     /locus_tag="ACFI8B_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834448.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(5490..5687)
                     /locus_tag="ACFI8B_001222"
     CDS             complement(5490..5687)
                     /locus_tag="ACFI8B_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834449.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(5697..6713)
                     /locus_tag="ACFI8B_001223"
     CDS             complement(5697..6713)
                     /locus_tag="ACFI8B_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC3834450.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(6812..8026)
                     /gene="gltS"
                     /locus_tag="ACFI8B_001224"
     CDS             complement(6812..8026)
                     /gene="gltS"
                     /locus_tag="ACFI8B_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC3834451.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(8279..8560)
                     /locus_tag="ACFI8B_001225"
     CDS             complement(8279..8560)
                     /locus_tag="ACFI8B_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834452.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(8579..9703)
                     /locus_tag="ACFI8B_001226"
     CDS             complement(8579..9703)
                     /locus_tag="ACFI8B_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3834453.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(9766..10773)
                     /locus_tag="ACFI8B_001227"
     CDS             complement(9766..10773)
                     /locus_tag="ACFI8B_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC3834454.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(10766..11767)
                     /locus_tag="ACFI8B_001228"
     CDS             complement(10766..11767)
                     /locus_tag="ACFI8B_001228"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3834455.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGRTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(11798..14071)
                     /locus_tag="ACFI8B_001229"
     CDS             complement(11798..14071)
                     /locus_tag="ACFI8B_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC3834456.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            14186..15202
                     /gene="galE"
                     /locus_tag="ACFI8B_001230"
     CDS             14186..15202
                     /gene="galE"
                     /locus_tag="ACFI8B_001230"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC3834457.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            15203..16243
                     /gene="rfbB"
                     /locus_tag="ACFI8B_001231"
     CDS             15203..16243
                     /gene="rfbB"
                     /locus_tag="ACFI8B_001231"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC3834458.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            16348..17214
                     /gene="rfbA"
                     /locus_tag="ACFI8B_001232"
     CDS             16348..17214
                     /gene="rfbA"
                     /locus_tag="ACFI8B_001232"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC3834459.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKHLEKT
                     AYGQYLLHLIGK"
     gene            17353..17910
                     /gene="rfbC"
                     /locus_tag="ACFI8B_001233"
     CDS             17353..17910
                     /gene="rfbC"
                     /locus_tag="ACFI8B_001233"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC3834460.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(17954..19972)
                     /locus_tag="ACFI8B_001234"
     CDS             complement(17954..19972)
                     /locus_tag="ACFI8B_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC3834461.1"
                     /translation="MNKSLSGSVEKYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLVLLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(20167..21288)
                     /gene="dnaJ"
                     /locus_tag="ACFI8B_001235"
     CDS             complement(20167..21288)
                     /gene="dnaJ"
                     /locus_tag="ACFI8B_001235"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC3834462.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         5350 a         5907 c         5153 g         4946 t
ORIGIN      
        1 cacggcgaga ttccggcata gtaaaacaag atttggattt ttcttgcggc gcggcttcga
       61 ttgccacgtt actgaataat ttttatggca gacattattc tgaagcggaa atcttagaca
      121 aaatggataa aacccaaatg cgtacttctt ttgatgatat gcaacgcata atgcccgaac
      181 tgggttttga agcacaaggt tatgctttgc catttgaaca gttggtacaa ctaaaaattc
      241 ctgtaattgt gtatttaaaa taccgtaaaa acaaccattt ttcggtattg aacggtatta
      301 acggagaaac tgttttgctg gctgatccat cgctggggca cgtctcaacg agcaaatcac
      361 aattcttgag cgcatggaaa acacgtgatg gcgaaatgga aggaaaaatt ttagccatcg
      421 tgccaaaaaa tactgatttt gttagaaatc agatgttttt taataaaaat cccgttcgtc
      481 aaacacgttt tacggtagaa caaatccaaa tgcggcaaaa acgataataa accgattttt
      541 gcccatttat tttatatagt aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
      601 tacggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg
      661 cgaggcaacg ccgtactggt ttttgttaat ccactataaa agcaaagaaa ccgccattag
      721 cactacaccc ccccaagctg cggctgccaa acacatcggc tgcttctcga cgcacccgct
      781 gaaaaatgcg ccctatccgt ccgccttgcc cgcaccccct gcctttgccg ccctgcccga
      841 tgccgtctga agcctgaacc gccccaccag cccgatgatg cacgacaccg ccggaaaggg
      901 catacacaaa gccgtcagca cataacgcgc attccccgta ttgaccacgt ccgcgccctt
      961 cagcagcagc ggcagcgcgt tcgcccactc ccacagcgaa ggcgcgctga acgccaatac
     1021 cgccaccagc cccaacatcc ggaacacccc gatttccaag acctgccgcc gccgcaccga
     1081 acggttgagc agaaacacaa ccgccaaccc gaaacagacc accgccgcca taccgccgtt
     1141 ggcgacaatc tgcaaactgt tgaacgcaat atcgggcgta accgccaaca cgccttccga
     1201 atccggcacg gcacgctgca aatccaaacc ctgcaacaca ttcaacacaa aaccgtagat
     1261 caaatcaaaa aacaccacgc ctatcggcaa agcactcaaa acccgccaca tcttcctgtc
     1321 cgaccaaccg ttcaaaacct tcattttacc cgaccgcgca agccgccgaa caaaaaataa
     1381 atttagccta aatcataaaa ccgtgtaatt ttgcaccaaa aaaggcggtt tgcaggtaaa
     1441 atatcaaacc attaatttaa atcatatttt tacagaatat tccgccgcgt tcaccaaatg
     1501 gacatcaaac cggttccaaa tttctgtaat tttgcaacaa aataccgcaa aacacccgat
     1561 tgagaccgaa aggactttca tatgaaccag acaagccgcg atctgacccg catcagccac
     1621 aacaccaaaa tcgtcgccac ccttgggccg ggcagcaaca acgtcgaact gttggaagac
     1681 atgatccgcg tcggcggcct gaacgtcgtc cgcttcaact tcagccacgg cacgcccgaa
     1741 ttccatcagg aaaacgcccg catcgtgcgc gaggcggcaa aacgcgccgg acaggaaatc
     1801 gccatcattg ccgatctgca gggcccgaaa atccgcgtgg gcaaaatcgc cggcggcggc
     1861 atcgaattga acaaaggcga aacattggta ctcgatgccg cgctcgaagg cgaaggcacg
     1921 cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg acgttgccgc aggcgatgtc
     1981 ttgtggctgg acgacggcct gctgaccctg accgtggaat ccgtcgaagg cagcaggatt
     2041 atcacaaggg tggaaaacag ccacatcctg aaaagcaaca aaggcatcaa caaacgcggc
     2101 ggcggtttgt ccgcaggcgc gttgaccgaa aaagacttcc gcgacctgaa aaccgcgatt
     2161 gccatcggtt gcgactatct cgccatcagc tttgtgaaat ccgccgaaga cctgcacatc
     2221 gcgcgcgcca aagtcgaaga ggaaatgaag ggcagcactg ccgtccgccc cggtttggtt
     2281 tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg aaatcatcct tgccggcgac
     2341 ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg gacacgccgc cgtccccgcc
     2401 ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc gcttcagcat tacggcgacg
     2461 caaatgatgg aatcgatgat taccaacccc gtgccgaccc gcgcggaagt cagcgatgtg
     2521 gcaaacgcgg tattggacgg caccgatgcg gtgatgtgtt ccgccgaaac cgccgtcggc
     2581 gcgtatccgt ttgaaaccgt cagccaaatg gcgattatct gcgcggctgc ggaaaaagag
     2641 caggattcgc tcaacggcgt tgccgaacag acggagtatc ccgaagccgt cagcaccaac
     2701 ttagcgattg ccggcggcgc ggtcagcgtg gcgcgcgcgg ttcacgccaa agccatcgtc
     2761 gccctgaccg aaagcggttc gaccgccttt gaaatcagcc gccacaatat taccctgccg
     2821 attttcgcgc tgaccccgag cgtttccgcc caacgccgta tggcgatgta ccggggcgtg
     2881 cgcccgctga ttttggcaac cagcaccgac cacgacacgg cgttgaacga agtcgaaacg
     2941 atgctggtgg aacacaacat cctgcattcc ggcgaccagt acatcatcac cagcggctcg
     3001 caaatgcgcg aatccggttc gaccaatacg ctggaagtgc tgcgcgtcaa ataatccgcc
     3061 ctgagtggaa aatgccgtct aaagccgata ctgtcgggct tcagacggca ttttatttgc
     3121 attccgtcag gtaaaaccga aagaattgtg caaatccggc aacatcggat aaaatcgagt
     3181 gcctatacta aagcgaaaca aggcttttcc gactgccttt tttatttgtc caccgtccgc
     3241 ctttttacgg aaaccgaaat gacccctttc acactgaaaa aaccgtcctg ctgctcggca
     3301 ctgcctttgc cgccgcatct gtccacgcat ccggctacca cttcggcaca cagtcggtca
     3361 acgcgcaaag cacggcaaat gccgccgacg cgtcgaccat cttctacaat cccgccggcc
     3421 tgaccaaact cgacagcagc cagatttccg tcaacgccaa catcgtgctg cccagcattc
     3481 attatgaagc agattccgcc accgacttta ccgggcttcc cgtccaaggt tctaaaaacg
     3541 gcaaaatcac caaaaccacg gtcgcacccc acatttacgg cgcatacaaa gtcaacgaca
     3601 atctgaccgt gggcttgggc gtgtacgtcc ccttcggctc tgccaccgaa tacgaaaaag
     3661 attccgtgtt gcgccacaac atcaacaaac tcggtctgac cagcatcgcc gtcgaacctg
     3721 tcgccgcgtg gaaactcaac gaacgccatt ccttcggcgc aggcatcatc gcccaacata
     3781 attccgccga actgcgcaaa tatgccgact gaggaatccc aaaaaaagcg caaatgctgc
     3841 aagcaacacc ttctaatcct actgccgctg ctcaaatcaa ggccgacgga cacgccgatg
     3901 tcaaaggcag cgattggggc gtcggctacc aactggcgtg gatgtgggac atcaacgacc
     3961 gcgcgcgcgt gggcgtgaac taccgttcca aagtttcaca cacgctcaaa ggcgatgccg
     4021 aatgggcggc agacggcgcg gcggcgaaac aacagtggaa tgacaatatg ctcacaccgc
     4081 tcggttacac ggcgaatgaa aaagcccgcg tcaaaatcgt aacgcctgag tctttgtccg
     4141 tacacggcat gtacaaagtg tccgacaaag ccgacctgtt cggcgacgta acttggacgc
     4201 gccacagccg cttcaataag gcggaactgt tttttgaaaa agaaaaaaat attgctaatg
     4261 gcaaaaaatc cgaccgcacc accatcaccc ccaactggcg caacacctac aaagtcggct
     4321 tgggcggttc ttatcaaatc agcgaaccgc tgcaactgcg cgtcggcatc gcttttgaca
     4381 aaccgcctgt ccgcaacgcc gactaccgca tgaacagcct gcccgacggc aaccgcatct
     4441 ggttctccgc cggcatgaaa taccatatcg gcaaaaacca cgtcgtcgat gccgcctaca
     4501 cccacatcca catcaacgac accagctacc gcacggcgaa ggcaagcggc aacgatgtgg
     4561 acagcaaagg tgcgtcttgc gcacgtttca aaaaccacgc cgacatcatc gacctgcaat
     4621 acacctacaa attcaaataa acgttacacc gtttgaatat aaaaatgccg tctgaaacag
     4681 atttccgttt cagacggcat ttttatccgg cagccgatac gcctatctcg gtattcggga
     4741 gaatatatac agaacctcct tcatatcaaa atggtaggga cgctcgggca acaggacggc
     4801 ggcaggcagc ttggtgatgc cgttcaaacc gcgcgggaac agcgcaatcc gctctttgcc
     4861 cttttccgca taatctgctt tgcggttgtg gatggaaact acttcgcgat ggcggtaaag
     4921 cagcgtgttc atttcgatat aggcttcgtt gtggatggct gccgactgtt tctgcaacac
     4981 gtcaatctgc ctgttgagct tgtcgttggc tttcatcagc ctgttgaaga acgacggttg
     5041 ctcctgcagt ttggcggtac aggcttcgat actttccgat accggcccga actgttcgag
     5101 gtatttttcc agacagtttt ccaagtcggt caggcagccg gccatcttgc gggaaaattc
     5161 ttcttcaggc tttccctgcc gaatggacaa tatagcggat taacaaaaat caggacaagg
     5221 cgacgaagcc gcagacagta cagatagtac ggcaaggcga ggcaacgccg taccggtttt
     5281 tgttaatcca ctatatttgg gaggggcgta accccttccg aatcaggacg gcacataggg
     5341 cgacgcttta tgtgccgtcc tgtgtgttga aacatcagca taggaaacac aggaaaagcc
     5401 ggctgttttg cgcctgcgta ccgtattggt caaaatgccg tctgaaaccg acaacagggt
     5461 ttcagacggc atttccgtca attttccctt tatctgccga acaggttggc gatattttcc
     5521 atctgttctt cggtaaaacc gttttcgccc aatctggcgg caactgccca ttcgcccgac
     5581 aaaccgtggt ccgcaccgag ctgccgccat ttttcgactt tggcttcggc tgtggttttt
     5641 tcgggcagcg gtttgacggg aatcctgctt ttggggacgg tattcatcgg tttgccctaa
     5701 tcgatggaat agccgcgaag atactgtatc cgttcgcgcg tcatccgcgt gtcgcattgg
     5761 agcttgaggt attcggcgta ttcctgccgg tctgcctgcg cggcggcttg tcggcagttg
     5821 ctgatttttt cctgcgccca cttgcgctgt tcgccgacca actctttttg cacgtcggta
     5881 tcgagtcctc cccaaagttt ggtaatttcg gattccgcac gttggttttg tacgcgcgcc
     5941 tcttccactt cgccccgtga tacggtaacg gtatcggcac gctcgacgtc gtcgggatgc
     6001 aggatttcgg gttcgggcgc gccttctgcg gcttggggta cgcccgcatc gccgccggcg
     6061 gcattgtgtt ccaaaatgtc ttcgggggtg ggtttggacg gttcttcttc acgggctttg
     6121 ccgctcaaaa ccctgaccgc gtcttctttt gtcaccgcct tgccgtctat catcacgatg
     6181 ctcttcacgc cgtaaggcag caacgcggca gacagcgttt gcgccgccat accgaccgtg
     6241 ttgtcgataa atgccgtccg agcgtctttg gcgggcagga agcggacggc tgccgtcaat
     6301 acgccgtctt taaactcgac attgccgccc gtcttctgct gcacgatgtc tgccaaagac
     6361 gtttccccat acagcagggg gctgtttgcc tcggcatcgg caagcgtttc agacggcacg
     6421 gtaatgttca aatcggcgat acagaacgtg cgcccgcctt cctgcgtttc cgaagcgtgt
     6481 tccaaagaaa acgccaaacc gtaggcggcg gcgataattt tgtcggcatc gacaaactgc
     6541 ctgccgtctt cgcgcgcgaa agaacgcgct tcctgcgtga gcgtttcctg aatactgccg
     6601 cgtatgtcct gcaacacggc ggggttggcg cattccaacg ccttgggcgg ttcttccctg
     6661 ccgcacgctg caagcagcag ggcaaacggc agcgcaatga gtttccgata catgatgttc
     6721 cttccaaacg gtgcagaccg atggcggcta tcatagcaaa agcacctgca ggcggtaaac
     6781 cttgcaggcg ctttgtcgga aaggcggaaa atcagccttt aaagaaattc acaaaaccgg
     6841 tgagaatcgc ggcattaatc aaatcgacaa agaacgcgcc gaccataggc acaatcaaaa
     6901 acgccttatg cgacgcgccg aaagtatgcg tgacggactg catatttgcc accgccgtcg
     6961 gcgttgcgcc caaaccgaaa ccgcagtggc cggcagccaa tactgcggca tcatagtcgc
     7021 gccccataaa gacataggta acaaaagtcg cgtacaaaac catcactgcg gtttgtactg
     7081 caagaatcac ggttacagac cccgccaaac cggtcagctc ccacagtttc aaattcagca
     7141 acgccattgc caagaaaagc gaaagcgaag cattgccgaa cacatcgatg gcacggtcga
     7201 acatattgac cttgaatgcg gcggtaagga tgttgcggat aaccacgccg ccaaacagac
     7261 accacacgaa tttgggcagg tcgaacaggt attctttgtc gaagccgtcc ataatctcgg
     7321 caaacgccag acacgcggca aacatggcaa gcgtttcaac ggcagattcc gccgtaatca
     7381 ggcgggtgcg ttttgcctgc tcgaacacgt cgtccgcgtt gtcgtcctga tcctgttttg
     7441 tgttttcaac cggtttgcgg cccattttgt tgatcaggcg gcgcgcaacc ggcccgccga
     7501 tcaggccgcc gaacaccagc ccgaaagtag ccgaagcaat acccaaaccg gttgcgccga
     7561 ccaagccgta ttgcgtttca aaattaggtc cccacgcacc tgacgtgccg tgtccgcccg
     7621 tcagcgacac cgaaccggta atcagaccga tgagcgggtc caaacccaaa gccgtagcca
     7681 gtccgacccc gacaaagttt tgcaccaaga taaatccgcc cacaatcgcg gtaaaaacca
     7741 ccagcggcaa accgcccgcc ttcaaacggg aaaaatccgc gctcaagccg atggacgtga
     7801 agaaaatcag cataaacgca ttttgcagcg gtttctcaaa tttgaagctc acgccgtacg
     7861 cctcgtgcag cgcaaacagg ataatcgcgg caatcagccc gcccgccacg ggttcgggga
     7921 tgttaaaatc acgcaagatt ttgattttct taaccaaaac cttgccgacc aacaaaacca
     7981 aagtggcggc aatcaaggtg taataactgt taaacgccca ttccatatcg tttgacctcc
     8041 taaaatgttt ttctttatat ccgcccgaag gggacgcaag cgcgaagata ttaggggaat
     8101 agttaaggct tgtcaaagaa tcgccgtttt ttatagtgga ttaaatttaa accagtacat
     8161 cgttgactcg ccttgccgta ctatctgtac tgtctgtggc ttcgtcgcct tgtcctgatt
     8221 taaatttaat ccactataca actatttttt acaaacaaaa tgccgtccga aactccgttc
     8281 agacggcatc atgggaatac acatatttga aataacagaa aaagcggggg caaaacccgc
     8341 cgctgcatct gtcgctgtac atccctgctg tcggacttac cgcttcaact tcgcaaacgc
     8401 atccgccatc gccgaattgg ttgggacacg ctcgttgcgt tgtggtttgc ggccggctgt
     8461 ccgttcgcgg ctgcggtttt tccagctcgg acagaatgtc cagaatggtc ggcagtccga
     8521 agtgttcgtc ggtgaagtcg gacgctttga tttgcttcac gcgctcgcgg ttaactgcct
     8581 aaatatgaaa ttcttggtct tcttgtagca taagttctcg atgatatgcc aagtaagatt
     8641 tacgtttgtc gttgagcaga ttgttatcta gcttcacatc acaccaacgt cgccaaacat
     8701 catctgataa ccgcttagat ttcaccatat ttccttcatc atcaaacgaa atatatttca
     8761 aatcaaatag gctatccaag gtacgactta atagcaagcc gttatttggg tcgtatgctt
     8821 cggtatcatc cgacaggatg aaaggcttaa tatgactggc aatcaataca ggataggcaa
     8881 gtttttccag catacatttc acattaccgt aatgttcttc actttcttct tgaagttggt
     8941 ttttgtatag gcgatgtaaa taagggtcgc gtttccttac agtaatttca tccaaattac
     9001 caaaaatacg ttgggcatcc tcagcaaaat ataagtcatc acctactcgt tttaagtcgt
     9061 ctaatttgtc taataggttc cataaataac ttatttgatt gtattttcgt tcaataaatc
     9121 ctgattctat tccctgttga aaataatcat tcaattctgt ttcggttaga taatcttgtt
     9181 gaaaggtttt taagtctact aacatcatgg cggcaatttc ttttttattt agcttgcctt
     9241 gtggatgctc gaccagcgtt ttaatcagga aatttatttg tcgaatatta gagttttcag
     9301 ttactgaacg ctgaaagcca gagtgcgtat atacgatttt ggaaagtaga gtttgtcgtt
     9361 ttcggttagt acgagcttgt acataatctc gtgataaagg tgtatagctt gataaaaacg
     9421 gttctataaa tcccattttt acaaattgat taatagcttt acgaaccgag gccaaattca
     9481 tggccttgct tgttctctgc gaagccataa cggaatcttg ggcaatgttt tgctgcaatg
     9541 cttcatatag ttcgttgctg tatggctggt cttgattcgc atcaataaag tcaagacaaa
     9601 tattgagtgc agtacaaaat ttttgatcgt tataatttgt aaaggcattg gtaaacgccc
     9661 aataatcttc ataaatttgg ctgtttggct gattattact catctctgtc tccaagaata
     9721 ttcgatacca atattctgtt tttgtattta agtaatatct atcattcatg tattttctac
     9781 taaaaacaga atttccttat ggctttgctt cttttctttt cctgtaacct tataaacgtg
     9841 aggcgtttcc aagatttgta catttcgccc atttttacct aacatttctt ctaattcatc
     9901 tcggtttgga tatgaagcat tgttgtagct taacagccaa tatttaaatg gtttcaaatg
     9961 attgattacc cttttaaata attcgacagc ctgcttttta tccatgaaat gattggcaaa
    10021 tggtttgggt atggatgatg taatgtaact gtccaataat ccgtaaaagc caaaataatt
    10081 gttcatcgtg cctgtgtaag gtgggtctaa atagacggca tcagcttgta tattaggtaa
    10141 taaatcaaat atatcttgat taaaggcgtg atgccgccag ccattattga aaacagcttg
    10201 gttatagtca tccaaatttt gcagaaaatg actttgaaaa ctttcattgt ggtaggcgcg
    10261 tcgacggcca tatttagcat aactgtattc ttcatcacgc agttgtttaa ctttttccca
    10321 aggaattgtg aatcgtgaat agggcatttt acgtatcatg gcgcgacgca ttaaagtaaa
    10381 cgccaaagca cgtttatact ggttattcag cttccctata tttttacggt acaaatcaag
    10441 ttgttggcat tcatcgtgaa aatagaattt ttcggaataa cgctgactca taaaaccggc
    10501 atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca tttaatgttt catggttgtt
    10561 ttctattaat gctaaagcaa tttggtaatt aattgccaaa atatcgttag taatcacacg
    10621 atagccgcgt tttttggctt cgtaggcaaa ggaacagcct ccactaaata catctgcaac
    10681 tgtatctaca tcagacggaa gctggtcaca aatccaggaa gctattttct ctttattacc
    10741 gatatagtta attttcggat atttcttatt cattttcact catttttaaa atactttcag
    10801 caatagcttt tgccaataaa ggcggcacgg cgttaccaac ctgctgctgc tgggcaattt
    10861 tgctgccgca aaaaataaaa ttatcaggga aagattgtaa ggcagctaat tcacgaacgg
    10921 ttaaagcccg attctgttca tagtgaaaaa ctttgcgcat atctcctgta atacaaacgg
    10981 ctggtttgtt gctgttgtaa cggatgtatt tacggatatc acctgttttc ggacgtaatg
    11041 gttcaggaat atcgttacgg ttacctccat ttttaacaaa tgccattttt tctaacattt
    11101 gtgccgaatg attcatagct tcatgatttg caacgtgtgg attgctttcg ccagcagcca
    11161 gttttggaaa atgtcctatt gctgatccaa cagtctgatg ggaaatctgc aaaggttcgg
    11221 gaaaggaaat tttgccttta tccctcctcc cgataaatat cactcggcta cgtatctgag
    11281 ggacaccgaa atcggctgca ctcagtatct tacattccac cgaataaccg atattctgaa
    11341 atgcttgaat aatctcaata cgtgttctac ctgaattgtg tgtatagagt cgcgctacat
    11401 tttccataac aaaaaaatat ggttggacaa ttttaactat tcggacaaac tctttaaata
    11461 aatggttgcg tgggtcatct gtaaatgtcc gtccaatctt tcctgccata ctaaaacctt
    11521 gacaaggtgg tcctccaata accaaatcaa ctgattgtcc gttaagacaa ttggttaaat
    11581 cttgttcggt tagtgtggtt aaatcttttt gcagtaattg atgacggggg aagttggtac
    11641 ggtaagtctg acaataatca gactccattt caacagaaag caattgttgg aatccggctt
    11701 gttcaaaacc caaggatagg cctcctgctc ctgagaaaag gtcaatatag gtaagtggtg
    11761 tgtgcataag tcaaaatcca taaactcttc gattatttta ccgcttcagc ttcgcaaacg
    11821 catccgccat cgccgaattg gtcggggcgc ggtcgttgcg ttggggtttg cggccggctg
    11881 tccgttcgcg gctgcggttt tcagacggca ttttatgttt tgcgccgccc ggttcgtcat
    11941 ccaagcgcat ggtcagcgcg atgcgtttgc gtgcggcatc gacttccagc actttcactt
    12001 tcaccacgtc gccggctttc accacttcgc gcgggtcttg gacgaactta ttagacaggg
    12061 cggagatgtg caccaagccg tcctgatgga cgccgatgtc cacgaacgcg ccgaagttgg
    12121 cgacgttgga gaccacgccc tcgagtatca tgccgacttg caagtcgctg atttcgtgga
    12181 tgccttcggc aaacgatgcc gtctgaaacg caccgcgcgg gtcgcggccg ggtttttcca
    12241 gctcggacag gatgtccaaa atagtcggca gaccgaagcg ttcgtcggtg aagtcggatg
    12301 ctttgatttg cttcacacgc tcgcggttgc cgatgagttc ggcggcggta atgccttgtt
    12361 gcgccagcat tttggcgacg acgggatagg cttcggggtg gacggcgctc gcgtccagcg
    12421 gctctttgcc gccgttaatc cgcaaaaagc ctgctgcttg ctcgaaggtt ttttcgccca
    12481 agcgcggtac ttttagcaat ttttttcggc tgtcgaacgc gccgttttca tcacggtagg
    12541 cgacgatgtt ttgggcaagg gtttgattca agccggaaat ccgcgccaag agcggggcgg
    12601 aggcggtgtt cgcgtccacg ccgacggcgt tcacgcagtc ttcgaccacc gcgtccagcg
    12661 atttggcgag ccggctttgg ttcacgtcgt gctgatactg ccccacgccg atggatttgg
    12721 ggtcgatttt gaccaactcg gcaagcgggt cttgcagcct gcgggcgatg gacactgcgc
    12781 cgcgcaggga aacatctaag tcgggaaact cgcgcgccgc cagttcggac gcagaataaa
    12841 tcgacgcgcc ggcttcggaa acgacgattt tgtgcaggct gctttcgggc attccgcgca
    12901 ccagttcgcc cgcgattttg tcggtttcgc ggctggcggt gccgttgccg atggcgatga
    12961 gcttcacacc gtgttgttta atcaggcgcg acaacgttgc caacatattg ttttcttgat
    13021 gcaaatagac aatgaccgta tccagcagtt tgcccgtgtc gtccaccacg gcgcatttca
    13081 cgccgttgcg gtagccgggg tcgagtccca aagtggtcag ccgcccggcg ggcgcggcga
    13141 gcagcaagtc tttgagattg cgggcgaaca cggtaatcgc gtcagtgtcg gcagcttctt
    13201 tcaaacgatt gagagcttcg agttccagcg acaaaaagat tttcgcgcgc caagtcaggc
    13261 gcacggtgtc gcgcagccat ttgtggccgt ctgaaacctt gaagcgacgg gcgatgattt
    13321 gctcgtattc gctttgctgc gtaatcggcg tgtcgtcggg ctggtatttg agcgcgatgt
    13381 tcaacacgcc ttcgttgcga ccgcgcaaaa ccgccagcgc gcggtggctg ggcatggcgc
    13441 gcacgggttc gcggcggtcg aaataatcgc tgaatttttc gccttcggtt tctttgcctt
    13501 caacgacttg cgtgtggatt tcggcttcgt tccacagctt gtcgcgcagc gtgccgatga
    13561 gttccgcgtc ttcggcaaac tgctccatca gaatcgcgcg cgcgccatcc agcgcggctt
    13621 tggcgtcggg gatgttttcg ttcaggtagc cttgcgcggc ggcttccacg tcctgcggct
    13681 gttcggcaag caacacgtcc gccagcggct gcaaaccgtg ttcgcgagcg atttgcgctt
    13741 tggtgcggcg tttgggcttg tagggcagat acaggtcttc cagcgcggtt ttgttgtcgg
    13801 cggcttcgat ttgcgccctg aggtcgtctg aaagcttgcc ttgctcttca atgcttttta
    13861 acacaacggc cttgcgctct tccaactcgc gcaggtattg cagccgctcg gcaagctggc
    13921 gcagctgcgt atcgtccagc ccgcccgtgg cttctttacg gtaacgcgcg ataaagggga
    13981 cggtcgcgcc gtcgtccaaa agctcgatag cggcattgat ttgcacggta gtggcggaga
    14041 gttcttggga gaggattcgg gtaatgttca taaattgcct ccgtgatgcc gtctgaacag
    14101 ccgacggcgc aatgtgggta atcgtttgga aaagatgaag ataatactga tatactttgc
    14161 ccgatacaat ctgaaaggaa tgtttatgac cgtcctgatt accggcggca ccggctttat
    14221 cggttcgcac accgccgtct cgctcgtcca atccggttac gatgccgtga ttttggataa
    14281 tctgtgcaac tcgtctgccg ccgtcctccc gcgccttcgg caaattaccg gcagaaacat
    14341 accgttttat cagggcgaca tccgcgactg tcagattttg aggcagattt tttcagaaca
    14401 tgaaatcgaa tccgtcatcc attttgccgg tttgaaggca gtgggggaaa gcgttgccga
    14461 gccgacaaaa tattacggca acaatgttta cggcagcctg gtgctggcgg aagaaatggc
    14521 gcgcgcgggc gtgttgaaaa tcgtattcag ctcgtcggca accgtttacg gcgatgcgga
    14581 aaaagtcccc tatacggaag atatgcgccc gggcgatacc gctaatcctt acggtgcgtc
    14641 caaagcgatg gtggagcgga tgttaaccga catccaaaaa gccgatccgc gttggagcgt
    14701 gattttgttg cgctatttca acccgatcgg cgcgcacgaa agcggactta tcggcgaaca
    14761 gcccaacggc gttcccaaca atcttttgcc ctatatctgt caagtggctt cgggcaggct
    14821 gccgcaactg tcggtattcg gcggcgacta tccgaccccc gacggtacgg gaatgcgcga
    14881 ctacatccat gtgatggatt tggcagaagg gcatatcgcg gcaatgaagg cgaaaggcgg
    14941 cgttgccggc gtacatttgt tcaacttggg ttcgggacgc gcctattccg ttttggaaat
    15001 catccgcgcc tttgaggccg catccggttt gcacattcct taccgaatcc aaccccgccg
    15061 cgccggcgac ttggcgtgtt cctatgccga cccgtcccat accaaacaac aaaccggctg
    15121 ggaaaccaaa cgcggcttgc agcaaatgat ggaagattcg tggcgttggg tcagccgcaa
    15181 ccccggcaga tatggggatt aaatgcaaac cgcaggcaaa aaaaacatcc tcgttaccgg
    15241 cggcgcgggt tttatcggct cggcagtcgt ccgccatatt atccaaaaca cccgagattc
    15301 cgtcgtcaac ctcgacaagt taacctacgc gggcaatctc gaatcgctga ccgacattgc
    15361 cgataatccc cgctacgctt ttgagcaagt ggatatttgc gaccgcgccg aactcgaccg
    15421 cgtgttcgcg caataccggc ccgatgccgt gatgcacttg gcggcggaaa gccacgtcga
    15481 ccgcgccatc ggttcggcag gcgaatttat ccgaaccaat atcgtcggca cattcgacct
    15541 gctggaagct gcgcgcgcct attggcagca aatgccgtct gaaaaacgcg aagccttccg
    15601 tttccaccat atttctaccg atgaagtcta tggcgatttg cacggcacag acgatttgtt
    15661 tacagaaacc acgccatacg cgccgtccag cccctactcc gcctccaaag ccgccgccga
    15721 ccaccttgtc cgcgcgtggc agcggactta caggctgccc tccattgtca gcaactgttc
    15781 caataattac ggcccccgac aattccccga aaaactcatt cctttgatga ttctgaacgc
    15841 actttccggc aaaccactgc ctgtgtacgg cgacggggcg caaatccgcg actggctgtt
    15901 tgtcgaagac cacgcgcgcg cgctgtatca ggtcgttacc gaaggtgttg tcggcgaaac
    15961 ctacaatatc ggcggacaca atgaaaaaac caatctcgaa gtgatcaaaa ccatctgcgc
    16021 cctcttggaa gaactcgccc ccgaaaaacc ggccggcgtg gcacggtatg aagatttaat
    16081 tactttcgta caagaccgcc ccggccatga tgcccgctac gccgtcgatg cggcgaaaat
    16141 caggcgggat ttgggctggc tgcctttgga aacttttgaa tccggcctcc gcaaaaccgt
    16201 gcaatggtat ttggacaaca aaacccggcg gcaaaacgca taaaacggca actgccgttc
    16261 ggaacgctta ggtacgggca aatagttttc agacggcatc ccgacgcaat gccgtctgaa
    16321 aacccatcgc aaaggaagaa agaaaagatg aaaggcatca tactggcagg cggcagcggt
    16381 acgcgcctat atccgattac gcgcggcgta tccaaacaac tgctgcccgt gtacgacaaa
    16441 ccgatgattt attacccctt gtcggttttg atgctggcgg gaatccgcga tattttggta
    16501 attaccgcac ccgaagacaa cgctgccttc caacggctac tcggcgacgg ctccgacttc
    16561 ggcatccgcc tgcaatatgc cgttcaaccc agccccgacg gcttggcgca ggcatttatc
    16621 atcggcgaag aatttatcgg caacggcaat gtctgcctga ttttgggcga caatattttc
    16681 tacggtcaat catttacgca aacattgaaa caggccgccg ccaagaccca cggcgcaacc
    16741 gtattcggtt atcgggttaa agaccccgag cgtttcggcg tggttgaatt tgacgaaaac
    16801 ttcaacgccc tgtctatcga agaaaaaccg caacagccca aatccgattg ggcggtaacc
    16861 ggcttgtact tccacgacaa ccgcgccgtc gagttcgcca aacagctcaa accgtccgca
    16921 cgcggcgaat tggaaatttc cgacctcaac cggatgtatc tggaagacgg ctcgctgtcc
    16981 gttcaaatat tgggacgcgg tttcgcgtgg ttggacaccg gcacgcaaga aagcctgcac
    17041 gaggcggctt ccttcgtcca aaccgtgcaa aatatccaaa acctgcacat cgcctgcctc
    17101 gaagaaatcg cctggcgcaa cggttggctg acgaaaaaag atgtagaaac acgcgcaaaa
    17161 catttggaaa aaaccgccta cgggcaatac ctgctgcacc taatcggcaa ataaataccg
    17221 tctgaacgcc ccgacggctt tcagacggca tccaaaccgt gccgcgccgg caccttaacc
    17281 gacctgccgc acggcgcgca taaaaaatca aaccgcaaaa aaacagcggt aacgataccg
    17341 ccaaaggact ttatggacat catcgacacc gccctccccg acgtaaaact tttaaaaccg
    17401 caagtcttca cagacgggcg cggttttttt atggaaacat tccgcgacgg atggttcaaa
    17461 gaaaatattg ccgatcgaac tttcgtgcag gaaaaccact ccaactccag caagggcgta
    17521 ttgcgcggcc tgcactacca aaccgaaaac acccaaggca aactcgtacg cgtagttgtc
    17581 ggcgaagtgt tcgacgtggc cgtcgatatg cgcgaaggtt cgccgacttt cggcaaatgg
    17641 gcgggcgcaa ccttgtcggc gcaaaaccga taccagcttt ggatacccga aggcttcgca
    17701 cacggttttt gcgttttggg cgacgcggcg gaagtcgtgt acaaatgcac ggactattac
    17761 aaccctgaaa ccgaacaggt tttaatatgg aacgacccgg caatcggcat aggctggccg
    17821 cttcaaaccg cgccgctgct ttcgcccaaa gaccttgccg gcaaaacgtg ggcacaagcc
    17881 gaaaagctcc gccttacgct ttcccgataa aaaatgccgt ccgaacgttt cagacggcat
    17941 tttctccgac aggctaccta cccgccttca gtacgcgctg tgcaaagaaa aacatcccgg
    18001 taacgaagaa cgccaagccc aaccaagaag cggcggcatc ccagttttgc agattcaacg
    18061 cgagcggcgc atccgagccg ccgttggtca cagagaaggc aataataaac gccataatta
    18121 caccaatcag gctttcaccg acaatcaggc ctgccgcgaa aagcgtaccg atacggtcgg
    18181 cgtttttcag acggccttcg cggttttccg ccttcttgcc aatgatgtgt ttcaacaccg
    18241 ccgccaacac cgcgcctgcc acgatgggca tattgacgga cggcggcaga taaatgccca
    18301 taccgaccgc aaggacgggc agggcaagtt taccgcctga tgattttttc aacaccaaat
    18361 cgacgacgat taataccgct ccaatcgcga taccggtaaa gatgtacgcc cattcgaggt
    18421 tgtgggcgaa aatgcccgac gcaatggtcg tcatcaaagt cgcttgaggg gctgccaaag
    18481 cctgcgccgc atccatgcct tcgcgcggca ttgcgccggt aaagccgtag gcttcgtaaa
    18541 gcagttccaa cacgggcgaa ataaccagcg caccaacgat acagccgata atcagggcga
    18601 cttgctgccg ccaaggcgta gctttgagca ggtagccggt tttcaagtct tgcaggttgt
    18661 cgttggaaat cgaagccacg cagattactg ccgatccgca aaaaagcgtc aacgccagta
    18721 agaatttgcg gttggcctca tccgccaaca aacctccgga ttcgcctacc agcagcaaaa
    18781 ccagtgaaat aacgacgatg gacacgatgc ccacgccgga aatcgggctg gaagacgagc
    18841 cgaccaaacc tgccatataa ccgcaggcgg cggcgaccaa aaagccgatg acggaagcca
    18901 aaagcgtgca aaccgccacc aaaagccacg ccatgccgcc cgtaatgtgc gaatcgccga
    18961 taaagtggta aaacgacacg cctagaataa acatcataga caacacccag aaaatcatgg
    19021 ctttaggcga caaatcctgt tcggcgcgtt ctgtagcggg cgcaccgccg ccaaaactct
    19081 tgaacgacat cttcatgcct tccaccattg gcttgagcag catcaaaagc gtccaaaccg
    19141 ccgcgatacc tatggttccc gcaccgataa aacgcacttt ttccttccac agcttcatcg
    19201 caaacgccgc catttccata tcggaaggtt gcggaatgtg tgaggagaaa tacggcacgg
    19261 caatacccca agcaatcgaa atgcccagca ggatggcgat gccgcccgtc agtccgacca
    19321 aataacccgc gcccaacaag accagtgaaa agcccatcgg cagttggaaa attgccgtac
    19381 cgcttttaaa ccaataactc gcgctatcgg caatcacacg cagacctccg gcgcaaaagc
    19441 tcatcaatcc cgccaacgcg ccgccggccg ccagctcttt gataccgctg ccgccctgac
    19501 ggtcatcccc ttcttcatga ccgcccactt tcaaaatttc agcagccgcc acaccttccg
    19561 gataaggcaa atcgcttttc accaccatcg cgtaacgcag cggaatggtg aaaatcaccc
    19621 ccaaaatccc gccggcaata cataaaagcg tcgtctgcca aaacgggaaa ccgctccagt
    19681 agcccgccat cagcaaaccg ggcaggacga agatgatggt tgaaagcgta cccgcagccg
    19741 aggcttgggt ctgcaccatg ttgttttcca aaatattgct gcctttgaaa aattttaaaa
    19801 ccgccatcga aatcaccgcc gccggaatcg acgaggcaaa ggtcagcccg actttcaaac
    19861 cgaggtaaac gttagacgca gtaaaaatta cagtgatcaa tgcgccgagt atcatgcctc
    19921 ggagcgtcag ttcgcggtat ttttctaccg aaccggataa agatttattc attattcttc
    19981 ctttgacaac agacgttcac atattgttgg catcacgcca tgatgtcaag ttttaaaaag
    20041 aacagttaaa aacaattatc ccaccctgcc catacccaat tgaaaataaa aactatttcc
    20101 aaacgaacaa aaacagccgt atcagggagg ttccccgata cggctgcttg tttccgaacc
    20161 ttaaaatcaa tcaaacaaat cgcgcagctt gtctaaaaac gatttcttgc gcggcgtttg
    20221 gttttccaag cccgtagaaa tccgctcaaa ttcttccaaa agctcttttt gacggtcggt
    20281 caaattgaca ggcgtttcga caacaatatg gcagtacaaa tcgccggtcg cgctgctgcg
    20341 taaagatttg acacccttac ctttcacgcg catcctcctg ccggtttggg tttctttggg
    20401 gacggtgagc ttgactttgc cgtccaaggt cggcacttct aactccccgc ccaaagcagc
    20461 cgtggcaaaa ctaatcggca gttcgcaatg caaatccaaa ccatcgcgtt ggaaaatctt
    20521 atgcgcccga atgcggacgg ttacatacag gtcgccggca ggcgcgccgt gcatacccgg
    20581 cccgccttca ccgctcaaac ggatacgctg cccgtcatcg ataccggcgg gaatattgac
    20641 ttccaccgtc ttgaccgcct tattccgccc cacgccacgg catttgacgc aaggttcttt
    20701 aatatgtttg cccgcaccgc ggcaggtcgg acaagtctgc tgcatacgga aaatcgcctg
    20761 ctggatgtgc accgtacccg aacctttgca agtcgggcag gtttccgggg atgcccccgg
    20821 tttcgcgccg ctgccgttgc agacatcaca cgcttcataa gtcggaatat tgatgcgttt
    20881 cttcacacct tttgcggctt cttcaagcgt gatttcgata ccgacctgca cgtcctcacc
    20941 ctgataatca ggctgggcgc gccccgaacc acctccaaac atttggctga aaatatcccc
    21001 aaagtcaaaa ccctgcgcac cgccaaatcc gccaaaccct ccgaagcccc cctgtccgcc
    21061 gccttcaaac gccgcatgac catactggtc gtacatggca cgcttttcct tgtcggacaa
    21121 agtttcatac gccttttgta cttctttaaa cttctcttcc gcctctttat tgtcaggatt
    21181 gcggtcggga tggtatttca tcgccaattt acggtaggct tttttaatct catcatcggt
    21241 agctgctctt gccacaccca gcgtcgcata aaaatcttga ttactcattt tttcatctaa
    21301 aaataaaatc acggcttaaa atgagggcaa ttgcgcaaaa cacaagacaa acagac
//
LOCUS       DAXFWM010000036        20352 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000036 DAXFWM010000000
VERSION     DAXFWM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20352
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.036"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            3..1250
                     /locus_tag="ACFI8B_001236"
     CDS             3..1250
                     /locus_tag="ACFI8B_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3834463.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            1346..1422
                     /locus_tag="ACFI8B_001237"
     tRNA            1346..1422
                     /locus_tag="ACFI8B_001237"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1380..1382,aa:Met,seq:cat)
     gene            1430..1505
                     /locus_tag="ACFI8B_001238"
     tRNA            1430..1505
                     /locus_tag="ACFI8B_001238"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1463..1465,aa:Ala,seq:ggc)
     gene            complement(1691..2512)
                     /locus_tag="ACFI8B_001239"
     CDS             complement(1691..2512)
                     /locus_tag="ACFI8B_001239"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC3834464.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(2545..3189)
                     /locus_tag="ACFI8B_001240"
     CDS             complement(2545..3189)
                     /locus_tag="ACFI8B_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC3834465.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAVVALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(3192..4055)
                     /gene="ftsN"
                     /locus_tag="ACFI8B_001241"
     CDS             complement(3192..4055)
                     /gene="ftsN"
                     /locus_tag="ACFI8B_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC3834466.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQTKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(4161..5657)
                     /locus_tag="ACFI8B_001242"
     CDS             complement(4161..5657)
                     /locus_tag="ACFI8B_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC3834467.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(5671..5991)
                     /locus_tag="ACFI8B_001243"
     CDS             complement(5671..5991)
                     /locus_tag="ACFI8B_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC3834468.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            6479..8005
                     /gene="putP"
                     /locus_tag="ACFI8B_001244"
     CDS             6479..8005
                     /gene="putP"
                     /locus_tag="ACFI8B_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC3834469.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            8257..11862
                     /gene="putA"
                     /locus_tag="ACFI8B_001245"
     CDS             8257..11862
                     /gene="putA"
                     /locus_tag="ACFI8B_001245"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC3834470.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRVLPSENGLDILQVFEEISCSINTTAAGGNAGLMAVAD"
     gene            12227..12925
                     /locus_tag="ACFI8B_001246"
     CDS             12227..12925
                     /locus_tag="ACFI8B_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3834471.1"
                     /translation="MQADLAYAAERITHDYPEPTGAKKDKKISTVSDYFRNIRTHSVH
                     PRVSVGYDFGSWRIAADYARYRKWNNSKYSVNIKRVKENNGSGKKLTQDLKTEHQENG
                     TFHAVSSLGLSAVYDFDTGSRFKPYAGVRVSYGHVRHSIDSTKKTTDVITAPPTTSDG
                     APTTYNANPQTQNPYHQSDSIRRVGLGVIAGVGFDITPNLTLDTGYRYHNWGRLENTR
                     FKTHEASLGMRYRF"
     gene            13160..13456
                     /locus_tag="ACFI8B_001247"
     CDS             13160..13456
                     /locus_tag="ACFI8B_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834472.1"
                     /translation="MQADLAYAYEHITRDYPEATGANQGKISTVSDYFKNIRTRSVHP
                     RLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
     gene            complement(14765..15001)
                     /locus_tag="ACFI8B_001248"
     CDS             complement(14765..15001)
                     /locus_tag="ACFI8B_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834473.1"
                     /translation="MKTANPHIVIPAKAALHTPPAAAIFTLKSRHSRESGNLGMKSSR
                     NLSEITETERTGFPPAREWRGKVGCGWDWWNDEI"
     gene            complement(15005..15160)
                     /locus_tag="ACFI8B_001249"
     CDS             complement(15005..15160)
                     /locus_tag="ACFI8B_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834474.1"
                     /translation="MPSESPSDGIGFPKHSAPFSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(15177..16973)
                     /locus_tag="ACFI8B_001250"
     CDS             complement(15177..16973)
                     /locus_tag="ACFI8B_001250"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC3834475.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTANEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIEWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            17432..18685
                     /locus_tag="ACFI8B_001251"
     CDS             17432..18685
                     /locus_tag="ACFI8B_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC3834476.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAAPSLMFDAGTVAALIMASCLIIVVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGREKPGNAELFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            18840..20351
                     /gene="lysS"
                     /locus_tag="ACFI8B_001252"
     CDS             18840..20351
                     /gene="lysS"
                     /locus_tag="ACFI8B_001252"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC3834477.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         4643 a         5794 c         5278 g         4637 t
ORIGIN      
        1 ccatgacagc acccgtacca acgtgtaata tgtcgggaaa tccaataaat ttacacaagc
       61 taacatttat catgctcctc ccctcccccg aagcacggca atcctcgctc aatctgcaaa
      121 ccctcattgc cgaagaaatc ggcaaacacg gcaattggat tccattttca cgttttatgg
      181 aattggtttt atacgctccg caatacggct actacaccgg cggcagccat aaaatcggca
      241 ataccgggga ttttattacc gcaccgaccc tcacccctct gtttgcacag acactggcac
      301 gccaacttca agaactcctg ccccaaacgg cgggcaatat ctatgaattc ggcgcgggaa
      361 ccggacagct tgccgccgat ttgttgggca gcgtttcaga cagcatcaac tgttactata
      421 ttattgaaat atcgccggag ctggcagcac gtcagaaaaa cctgattcaa gcccgtgcac
      481 cggaagcatc tcaaaaagtt gtccacttga ccgcacttcc cgaagcgttt gacggcatca
      541 tcatcggcaa cgaattactc gatgccatac ctgtcgaaat cgtccgtaaa aatgaaggag
      601 gattactcga gcacatcggc gtttgcacgg ataacggccg tttcgcctat tctgcaagac
      661 ccttgcacga cccgtcgctt tccacctcgg cttccctcta ttttcctcaa acagattatc
      721 cctataccag cgaactgcac ccgcaacaat atgcctttat ccgcaccctt gcctcaaggc
      781 ttgaacgcgg gggtatgata ttcatcgact acggttttga tgcggcgcag tattaccacc
      841 cgcaacgcaa tcaaggcaca ttgatcggac actaccgaca ccacgttatc cacaatcctt
      901 tcgacttcat cgggttggca gatctgaccg cccacgtcaa ctttaccgac attgcccaag
      961 ccggtacgga tgcaggactg gatttgacag gctatctgcc ccagtcccat tttctgctga
     1021 atctgggcat aaccgaactg ttggcacaga cgggtaaaac agattcggca gcctatatcc
     1081 gtgaagccgc cgctgttcaa aaactgattg accagcatga aatgggcgaa ctgtttaaag
     1141 tcatcgcatt cggaaaaaat atcggcatcg actgggcagg attccgcttc ggcgacatct
     1201 gccacaaact ccaaccctcg tgccgtctga atccgcttca gacggcataa actttttaac
     1261 atttaaaaac aagaaactaa ttcaaaatta aaaaatacgg cttgtcaaaa aaacaaaaaa
     1321 acatataata gctaattcac gaaacggcga attagctcag tcggttagag cagaggaatc
     1381 ataatccttg tgtccggggt tcgagtccct gattcgctac cagattttcg ggggtatagc
     1441 tcagttggta gagcgcttgc atggcatgca agaggtcagc ggttcgatcc cgcttacctc
     1501 caccagataa aaaagcacag accgtaaaag gtatgtgctt ttttattgcc tgattgccgg
     1561 aaaataaaga ataaaccact gccttcaaaa caggcaatcg actttaaacc tattgccccg
     1621 cctgtcctga ttttatcagc ccgccagaca aacccgacct gaataatgtc ttcaggtcgg
     1681 gtttatggtt tcattcccaa cttatccagc ccgacaacca caatattatg atgacaatgc
     1741 cgaaaacaat gcggtaatag gcaaacggga tatagttttt cttggaaaca aacttcagca
     1801 gtgctttaac cgctaccaaa ccggaaacaa aagcggcaat aaagcctatc aaaatcaaac
     1861 cgacatcatg cagggtgaaa aatcggtaat gtttcaggac atcataagcc gttgctgcaa
     1921 ccatcatcgg aacggccaag aaaaatgaaa actccgttgc cgttttccgc tcgattcccc
     1981 aaagcatccc gcccataacc gtactgcccg aacgggacgt acccggaacc agtgcaaaca
     2041 cttgggcaac accgatcatc aacgcatcaa tcggacgcaa tgcatcaaca tcggcaattt
     2101 taggctctgc tcggctttgg cgtttctcca cccacaaaat aaaaaaaccg cccaaaacca
     2161 gcatgactgc aacactcaag gggttaaaca gatactcttt gatttgtttg tcgaacaaca
     2221 gccccatcac ggcggcaggt ataaaagcaa tggcaagatt gaggacgaaa cggttggctt
     2281 tccggtcttt tcccacgcca tgcaacacat tgctgaaacg ctgccggtat tcaaacacta
     2341 ccgccaaaac cgcaccgagc tggatggcaa tttcaaaaac cttgtgattg ctgtgaaaac
     2401 caatcagatt gccgaacaca atcaaatgtc cggtgctgga aatcggtaaa aattcggtaa
     2461 aaccttctac caagcccatc atcagggctt tcaggacaat cagaaaatcc attgcttgcg
     2521 cttctttcgg atacgggagt tcggctattt ctgtacagca ggggtctgac gcttgcgttc
     2581 ttccctgact ttggcaacca attctttaat cgtatgaacg ccgccatcaa agccattatt
     2641 gaagataacg cggtattttc cgccgacaat aacggtcggc gtgctgtcaa taccgtattg
     2701 ttccgtcagt ttctgcattt ttaatgcgac gacggcagct tcgggggaat cataggcgcg
     2761 catcagtttt ttgccgtcaa aacctttttg agataaagcc cattttccgg caacagccct
     2821 gttttccaaa cggatttttt gttcgtaaac tgctttaaac acagcagagt ttgcctgata
     2881 tttcaaaccc gacagcttga ccgcagcagc cattcttgcc agaccgagca tttcaggccg
     2941 ccagaccacg tgctccgtcc gcagataagt atcagacggc aatgccttgc ccagtttcaa
     3001 taacaaagga tcgaaatgat ggcaatgtac gcaaaaatag ccgaaaaatt ccaaaacctc
     3061 aatttttccc ggctgttctt gaggaatggg tttatccaac acaagatagt cttccccttc
     3121 cgtcagggca tatgcctgcg cggacaacac tgccgacagc agcagcggca acagatgttt
     3181 aaacttcata attatttgcc ttcaatcgca cggatcaggc tggcaacccc atgctttttc
     3241 aactcgtcct gcattttttt caccgcatcg gcagacatat tgccgctttg cacgcggtaa
     3301 agcgttttat gtcccgcctg atagccgacc acttcggaag atatgcccaa gattgccagt
     3361 ttggcacgct gcccttccgc gctccggcgg tcggcatacg cgcccatttg cagataatgc
     3421 gttgcttccg ccttgccaaa cgttttcatt ttctgcactt ctttggcagc ggcactgcgc
     3481 gctttttcga tgctgccgct gttgaggatt tgttccgggg tcggtttggg tgcaactttt
     3541 tccttcgccg cctttttctc ttcttttgaa gcttttttct ctgtttcttt agacggtttt
     3601 accgcttgtt ttttaaccgt ttcggcatct ttcgtctgcg ctttttccct gacggtttgt
     3661 tcacgctctt ccgtcagtgc tttcttgcgc actgcctgtc cgtccggctc ttcccgttcc
     3721 ggctcgcccg ccttttcttc aacctcgtcg gctttgtcgg caacgggctg cttgtcggca
     3781 gctttttccg catccgactg ctctgcctct ttcgcaacat ccggttcgga caaggcgttt
     3841 tgatcggccg gttcaggttg gatgtcttcc ttaggctggt ttttcagttt caggatttcc
     3901 gtttctgcag gctgcttcga cggagccggg attttgaacg cattttgacc gccctggttc
     3961 agataaagca aaataccggc aataatgacc gttgccagta tcaaaccgaa gaagaaaccg
     4021 gacagacctt ttccggattg ggaaaatttg ttcataaaca taccttaatg tgtttcagac
     4081 ggcattaacg ccgtttgctt acgggcggat attctaacaa tatcgccata tttgggcaaa
     4141 acctgcttcc attcccgttc ctataaagca cgacggaagc ctatggcttt catgacgtgg
     4201 cttctgccga cttcttcatc gcccgccaaa tccgccaatg tacgcgccac acgcataatg
     4261 cggtggaagc tgcgggcgga aagagacaat ttttccagca ggctacccaa ggcttcctgc
     4321 gcttcttttt ggatgcaggc ttgggaatcg agttcgctga cgctcaaagc ggcgttgact
     4381 ttgccctgcc gtgcgtattg tttgccgcgg gcggcgatga cgcgttccaa aacggacgca
     4441 ctgctttcgc ccgcttcctg ctgcatcagt tcggcggcgg acaggctggg gacttcgatg
     4501 gtcaaatcga tgcggtcgag cagcggaccg gaaattttgc ttcggtaacg cgcgacactt
     4561 tcgggcgtgc agcggcaggg tttgacggga tgcccgagat aaccgcacgg acacgggttc
     4621 atggcagcga caagctggaa tttggcggga tagacggctt ggcgcgccgc gcgggaaatg
     4681 tggatttcgc cgttttccaa cggttcgcgc aaaacttcca aaactttgcg gtcgaactcg
     4741 ggcaattcgt ccaaaaataa aacgccgtga tgcgccaatg aaatctcgcc cggacgcgga
     4801 tccgagccgc cgccgaccat agccgccgcg ctggcgctgt gatgcggact gcggaaagga
     4861 cggttgctgt cgagttgttg ttggtggttg ggcaggagcg aacgcaatgc ccaaacttct
     4921 accaattcgt cttcggccag cggcggcagg atgccgggca gccgttggga gagcatagac
     4981 ttgcctgttc ccggcgggcc catcatcaag aggctgtgtc cgcctgcggc ggcgatttcc
     5041 aaagcaaggc gggcagagtg ctgacctttc acatcacaca aatcaggttg tccgccatgt
     5101 tcagacggca tttgcggtac ggaacattcg gtttgcgcca aaggttcgat accgttcaaa
     5161 tgagcggcga cttcgcccaa agagcgcgcg ccgtaaacgg taatgccgcg catcacggcg
     5221 gcttgtcctg cgttttcttc aggcaaaaca aatgcacgtt ttgcctgcat accctgccac
     5281 gccatcgcca acgcgccgcg cacggggcgc aacaaccccg acagtgccaa ttcccccgca
     5341 aactcgtatt ccgccagctt ttcgggtgca acctgccccg atgcggcaag gatgccgatt
     5401 gcaatcggca aatcgaaacg ccccgactct ttgggcaggt cggcaggggc gaggttgacg
     5461 gtgatttttt tggcggggaa ttcaaaaccg ctttgaataa tggcggcacg gacgcggtcg
     5521 cggctttcct ttacttccgt atcgggcagt ccgacgatgt tgaaatgcgg cagaccgttg
     5581 gcaaggtggg cttccacttc gaccaacggc gcattcatac cgctcaaggc gcggctgtaa
     5641 accaaggcaa gcgacatatt tcagacggcc ttattcgccg gcttcggttt gctgcctgat
     5701 ttcggcgacg gcttcttcgg cagcggcttc agccgcttcc aatgctgccc gttcgggatt
     5761 ttgcgcggct tcgagtttcg ccagacgtgc ttccaaagcc gccagtttgg tacgggtttt
     5821 aatcaaaacc tgctgctgga tgtcgaattc ttcgcgcgta accaaatcca tacggttgaa
     5881 cgcgccgccc agcatcgcct taatattttt ttccacatct ttggcagggc tgttggcgat
     5941 ggtttcgctg attttcgagc cgacttcttc aaaaagctgc ttgccgaaca taatctgcat
     6001 ccttcctgaa catatcaaat tcaagcggct attgtataag gaaaaatgcc gtctgaaaac
     6061 gggcggcgga taatcgtcaa aacataccgc gccccctttg cggttgctaa aattacactt
     6121 cgagacacct gccgcctgaa aacggatttc atattccgcg caacgcccga ttacattaca
     6181 agacactaca aaacaatatg ctgttttaaa tgatttttcc gatgcgcatc gtttcaaact
     6241 cggcttttta agccattaag tgctttgcaa acaacagaaa ttgggttatg ctgaaacgga
     6301 ttatttacaa tttcatatag tttcattaca tatcttattg taattgaaga taatttatcc
     6361 gaatcccctt tcgggtatcc ggattttccg ttgtactttc attagaaaaa catttcaggc
     6421 gcaagttgct tgcaatttca aagcctgtaa ttctgaagtt ttagacgagg aatagcacat
     6481 gaatcccatg tacatcactt ttgcaatcta tttggttgca gtccttctca tcgggcttgc
     6541 cgcttatttt tccacgcgca atttcgatga ttatattttg ggcgggcgca gcctgggccc
     6601 gtttgttacg gcgatgtcgg caggcgcgtc cgatatgtcc ggctggcttt tgatgggtct
     6661 gccgggcgcg atttatttga gcggtttgaa tgaggcttgg attgccatcg gcctcttggt
     6721 cggcgcgtat ttcaactggc tcttggtggc gggccgcctg cgcgtgcata ccgaatatgc
     6781 caacaatgcg ctgacgctgc cggattattt cttccaccgc ttcggcgcgg gcggacactt
     6841 gatgaaagtg gtttccgcac tgattatcct gtttttcttc acgatttatt gcgcctcggg
     6901 cattgtggcg ggcgcaaccc tgttccaaag cctgtttgaa ggtatgactt acaatcaggc
     6961 aatgtggctg ggcgcgggcg cgaccatcgc ctataccttc ttgggcggct tcttggcggt
     7021 cagctggacg gatacgctgc aggcttcttt gatgattttc gccctgattt taacgccggt
     7081 gatggtctat ctgggcttgg gcggcgcgga acagatgtct gccgcgattc aaagcgtcgc
     7141 cgcaggcacg ggcaaagaat acggcagcct gtttgccggt acgaccgtca tcggcatcat
     7201 ttccaccgcc gcctggggct tgggctattt cggacagccg cacattttgg cgcgctttat
     7261 ggccgccgaa agcgcgaaat cgctggtatc cgcacgccgc atcggcatga cttggatggc
     7321 gttgtgcctt gcgggcgcgg tagcggtcgg ttatttcggc attgcgtatt tcggtgcaaa
     7381 ccccgacaaa gtttcttcta tgagcggcaa ccacgaacgc atcttcatcg cgctttccac
     7441 cctgctgttc aacccttgga tcgccggcat cattttgagc gcgattttgg cggcggtgat
     7501 gtccaccctg tcctgccagc ttttggtttg ctccagcgcg attaccgaag acttctacaa
     7561 aggctttttg cgtaaaaacg cgcaacagtc ggaattggta tgggtcggcc gcctgatggt
     7621 attggcgatt gccgtgattt ccatcctgat tgcttccgat ccgaacagca aagtattggg
     7681 actggtgtct tacgcgtggg caggtttcgg tgcggcattc ggcccgattg tgattctgtc
     7741 cgtattgtgg aaacgcatca ctgcctacgg cgcgttgtca ggcatggttg caggtgcgtc
     7801 aaccgttgtt gtgtgggcgg aatgggtaaa aaaaccggct cgagcggcag gcgaaagcgg
     7861 cttgttgacg atgtacgaaa tcgtcccggg ctttattgtt tgcttgatcg ttgccgtgtt
     7921 ggtttccctg ttcaacaaag aaccttcgcg cgaaatccaa gaacgctttg aaaaagccga
     7981 cgcagattac cgcgccgccc gataaacccc tttcagacgg cacggcatcc cggttcgtgc
     8041 cgtctgaacc cgcttccggg ccaaacacaa ataaaccttg cctgcataca ggccttattt
     8101 gtgttttacc cgtttcattt tcaaagaaac ggaagcaccg attctgcccg catttgaagc
     8161 cttgtatttc atctgcggat ttattacgat attaccgtat tcaggccgca ccgatgccgt
     8221 ctgaaccccc cgaaaaaact ttggaggatc caaaaaatgt ttcattttgc atttccggca
     8281 caaactgccc tgcgccaagc gataaccgat gcctaccgcc gtaatgaaat cgaagccgta
     8341 caggatatgt tgcaacgtgc acagatgagt gacgaagagc gcaacgccgc ctccgagctt
     8401 gcccgccgtt tggttaccca agtccgcgcc ggccgcacca aagccggcgg cgtggatgcg
     8461 ctgatgcacg agttttcact ctccagcgaa gaaggcatcg cgctgatgtg tctggcagaa
     8521 gccctgctgc gtatccccga caacgccacg cgcgaccgcc tgattgccga caagatttca
     8581 gacggcaact ggaaaagcca tttgaacaac agcccttccc tcttcgtcaa tgctgccgcc
     8641 tggggtctgc tgattaccgg caagctgacc gccacaaacg acaaacaaat gagttccgca
     8701 ctcggccgcc tgatcggcaa aggcggcgca ccgctcatcc gccaaggcgt aaattacgcc
     8761 atgcggcttc tgggcaaaca gttcgtcacc ggacagacca ttgaagaagc cctgcaaaac
     8821 ggcaaagaac gcgaaaaaat gggctaccgc ttctccttcg atatgttggg cgaagccgcc
     8881 tacacccaag ccgatgccga ccgctactac cgcgactatg tcgaagccat ccacgccatc
     8941 ggcaaagatg cggcaggaca aggcgtttac gaaggcaacg gcatttccgt caaactctcc
     9001 gccatccatc cgcgctactc gcgcgcccaa cacggccgcg tgatgggcga actgttgcct
     9061 cgcctgaaag aattgttcct tttgggtaaa aaatacgata tcggtatcaa catcgatgcc
     9121 gaagaagcca accgtctgga gctgtctttg gatttgatgg aggcactcgt ttccgacccc
     9181 gatttggccg gctacaaagg catcggtttc gttgttcaag cctaccaaaa acgctgcccg
     9241 ttcgtcatcg actatctgat tgacctggcc cgccgcaaca accaaaaact gatgatccgc
     9301 ctcgtcaaag gcgcgtattg ggacagcgaa atcaaatggg cgcaagtgga cggcttgaac
     9361 ggctatccga cctacacccg caaagtgcat accgacatct cctacctcgc ctgcgcgcgc
     9421 aaactgcttt ccgcgcaaga cgcggtattc ccgcaattcg ccacccacaa cgcctacacc
     9481 ttgggcgcaa tctaccaaat gggcaaaggc aaagattttg aacaccaatg cctgcacggt
     9541 atgggcgaaa ctttgtacga ccaagtcgtc ggtccgcaaa acttaggccg ccgcgtgcgc
     9601 gtgtacgccc cggtcggcac acacgaaacc ctgctcgcct acttggtgcg ccgcctgttg
     9661 gaaaacggcg cgaactcgtc tttcgtcaac caaatcgttg atgaaaacat cagcatcgac
     9721 cgcctgatta agagcccgtt cgacaccatc gccgaacaag gcatccacct gcacaacgcc
     9781 ctgccgctgc cgcgcgattt gtacggcaaa tgccgtctga actcgcaagg cgtggacttg
     9841 agcaacgaaa acgtattgca gcagcttcaa gaacagatga acaaagccgc cgcacaagac
     9901 ttccacgccg catccatcgt caacggcaaa gcccgcgatg tcggcgaagc gcaaccgatt
     9961 aaaaaccctg ccgaccacgg cgatgttgtc ggtacggtca gctttgccga tgccgcgctt
    10021 gcccaagaag cggttggtgc agccgttgcc gcgttccccg aatggagtgc gacacctgcc
    10081 gccgaacgcg ccgcctgcct gcgccgtttc gccgacctgc tcgaacagca cacccccgcg
    10141 ctgatgatgc ttgccgtgcg cgaagcgggc aaaacgctga acaacgccat tgccgaagtg
    10201 cgcgaagccg tcgatttctg ccgctactac gcaaacgaag ccgaacacac cctgcctcaa
    10261 gacgcaaaag ccgtcggcgc gattgtcgcc atcagcccgt ggaacttccc gctcgccatc
    10321 tttaccggcg aagtcgtgtc cgccctggcg gcaggcaaca ccgtcatcgc caaacctgcc
    10381 gaacaaacca gcctgattgc cggttatgcc gtttccctca tgcacgaagc cggcatcccg
    10441 acttccgccc tgcaactcat cctcggcgca ggcgacacgg gtgcggcatt gaccaacgat
    10501 gccagcatcg gcggcgtgat tttcacaggc tcgaccgaag tggcgcgcct gatcaacaaa
    10561 gcccttgcca aacgcggcga caatcccgtc ctgattgccg aaaccggcgg acaaaacgcc
    10621 atgattgtcg attccaccgc acttgccgaa caagtctgcg ccgacgtatt gaactccgcc
    10681 ttcgacagcg cgggacaacg ctgctccgcc ctgcgtatct tatgcgtcca agaagacgtt
    10741 gccgaccgta tgctcgacat gatcaaaggc gcgatggacg aactcgtcgt cggcaaaccg
    10801 attcagctca ctaccgatgt cggtcccgtc atcgatgccg aagcgcagca aaacctgttg
    10861 aaccacatca acaaaatgaa aggcgttgcc aagtcttacc acgaagtcaa agccgccgcc
    10921 gatgtcgatt ccgaaaaatc cacgttcgtt cgccccatcc tgtttgaatt gaacaacctc
    10981 aacgaactgc aacgcgaagt cttcggcccc gtcctgcacg tcgtccgcta ccgcgccgac
    11041 gaactcgaca gcgtcatcga ccaaatcaac agcaaaggct acgccctgac ccacggcgta
    11101 cacagccgca tcgaaggcac ggtacgccac atccgcagcc gcatcgaagc cggcaacgtt
    11161 tacgtcaacc gcaacatcgt cggtgcagtc gtcggcgtac agcccttcgg cggacacggt
    11221 ctgtccggca caggccccaa agcaggaggt tcgttctacc tgcaaaaact gacccgcatc
    11281 cccgaatggg ttgccccgac cctgagccaa atcggacagg cagacgaagc cgcgctcaaa
    11341 cgcctcgaag ccctgattca caaactgccg ttcaacgccg aagagaaaaa agccgcagcg
    11401 gccgctttgg gacacgcccg catccgcacc ctgcgccgtg ccgaaaccgt ccttaccggc
    11461 ccgaccggcg agcgcaacag catctcatgg cacgcgccaa aacgcgtttg gatacacggc
    11521 ggcagcacgg ttcaagcctt tgccgcactg accggacttg ccgcctccgg cgtacaggca
    11581 gtggtcgaac ccgacagccc gctggcttcc tacaccgccg acttggaagg tctgctgctg
    11641 gtcaacggca aacccgaaac cgccggtatc agccacgttg ccgccctgtc gcctttagac
    11701 agcgcacgca aacaggaact tgccgcccat gacggcgcac tcatccgcgt cctcccttcg
    11761 gaaaacggac tcgacatcct gcaagtgttt gaagaaattt cttgcagcat caacaccaca
    11821 gccgccggcg gcaacgccgg cctgatggcg gttgccgact gattttgcca aaccgcccgg
    11881 gcgcggcccg tgaacaaatg ccgtctgaaa acctttcagg cggcatttta tagtggatta
    11941 acaaaaatca ggacaaggcg gcgggccgca gacagtacaa atagtacggc aaggcgggcc
    12001 aacgctgtac tggtttaaat tcaattcact atatgtgttg gcacatcgct ccaaacctga
    12061 tataatccgc ctttcaacat cagtgaaaat cgttcttttt agtcagttaa cattaaattt
    12121 cggagtcgaa aaatgaatcc agcccgcaaa aaaccttctc ttctcttctc ttctcttctc
    12181 ttccgcagcg caggcggcaa gtgaaggcaa tggccgcggc ccgtatgtgc aggcggattt
    12241 agcctacgcc gccgaacgca ttacccacga ttatccggaa ccaaccggtg caaaaaaaga
    12301 caaaaaaata agcacggtaa gcgattattt cagaaacatc cgtacgcatt ccgtccaccc
    12361 cagggtgtcg gtcggctacg atttcggcag ctggaggata gcggcagatt atgcccgtta
    12421 cagaaagtgg aacaacagta aatattccgt caacataaaa agggtgaaag aaaacaatgg
    12481 cagcgggaaa aaactgacgc aagacctgaa gacggaacat caggaaaacg gtacgttcca
    12541 cgccgtttct tctctcggct tgtccgccgt ttacgatttc gataccggtt cccgcttcaa
    12601 accctatgca ggcgtgcgcg tcagctacgg acacgtcaga cacagcatcg attcgaccaa
    12661 aaaaacaaca gatgttatta ccgccccccc cactacttct gacggagcac ctacaactta
    12721 taatgctaat ccacagacgc aaaaccctta tcaccaaagc gacagcatcc gccgcgtggg
    12781 cctcggcgtc atcgccggcg tcggtttcga catcacgccc aacctgaccc tggacaccgg
    12841 ctaccgctac cacaactggg gacgcctgga aaacacccgc ttcaaaaccc acgaagcctc
    12901 attgggcatg cgctaccgct tctgattccc cgataccgat gccgtctgaa ccttcagacg
    12961 gcatttttaa ggcgcaagat cgggcaaacg gcattttcag acggcataac tgacagtata
    13021 atccgaacat ccggccgctt tcgcgcggcc ttcagcatta tccgattttt tccgaaagcc
    13081 gaaccatgca atacaaaccc ctcctgctcg ccctgatgct tggcaggcgg cgggtgaaga
    13141 ccatgggcgc ggcccgtatg tgcaggcgga tctggcttac gcctacgagc acatcacccg
    13201 cgattatccc gaagcaaccg gtgcaaacca aggcaaaata agcacggtaa gcgattattt
    13261 caaaaacatc cgcacccgct ccgtccaccc ccgactcgcc ctcggctacg atttcggcgg
    13321 ctggcgcgtc ttttccgccc ccgccgttgc cgccggcctc caccaatccc ttcaatatta
    13381 ccgatcccgc cattgccgcc gattccgtca aatccatcaa ttccgccgaa tcacgcctat
    13441 tccccaaaaa ccttgatgcg gcgggctgaa gcccgccctg caaccctctc tatgcacccc
    13501 cttgcgagcc cgacactacg caacatcttg agaacccatc ctgtcaagaa tacccgaacc
    13561 gtcccgatac accgtaatcc taaaacccgc cattcccgcg caggcgggaa tccagtccgt
    13621 tcggtttcag tcatttccaa aaattgccgt agcgttaagt ttctagattc ccactttcgg
    13681 aggaatgacg cggtgcaggt ttccgtgcgg acggattcgt cattcccaca cagacaggaa
    13741 tccggatttg tccgcgcgga aacttatgcg ccgtcattcc cgcacaggcg ggaattcagg
    13801 tctgtcggca taggaactta tcggataaaa cggttgcccc aaccctgcgt tctagattcc
    13861 cactttcgtg ggaatgacga ttcaggtatt cctaatagca acccgccaca acccacctac
    13921 gcccatccta cgcatctgcc atacagccct gttccggagc gcgcggataa ggtgcgatgc
    13981 cgccggcggg catcatagtc ctctctccct gtgggagaga gctagaaagc tgcaaggctt
    14041 gtattttaga agactaaggg atttgggaaa gattgtcgaa attcggggag ccctctcccc
    14101 cacgggggag ggggcaggtt gcggcagatt tggcggttgc aggcggtttg aaaaacagat
    14161 tggatttaca accgttgatt tcaggtcgtc tgaaaagcaa aaagcagcct gcacaacctg
    14221 attagtttac aaaaccctta attccaacag cctccccgtc ctctctcccc gtgggagagg
    14281 gctggagaga gggcaacaag ccgcaaggct tgtattgggg cggttaagtt gttgggaaag
    14341 gttgccgcaa cttggagaat gccgcctccc cggccctccc cacgggggag ggagtgggtt
    14401 gcggcaggtt ttgcggttgc aggcggtttg aaaaacagat tggatttaca accgttgatt
    14461 tcaggtcgtc tgaaaagtaa aaagcagcct gcacaacctg attagtttac aaaaccctta
    14521 accccaacag cctccccgtc ctctctcccg tgggagaggg ctggagagag ggcaacaagc
    14581 cgcaaggctt gtattggggc ggttaagttg ttgggaaagg ttgccgcaac ttggagaatg
    14641 ccgcctcccc ggccctcccc acgggggagg gagcagattg cggcagattt ggcggttgca
    14701 ggcggtttga tttgcgaggg aatcggacgc gcaggcggga atccggattt gtccgcgcgg
    14761 aaacttatat ttcgtcattc caccaatccc acccacagcc cacttttccg cgccattccc
    14821 gcgcaggcgg gaatccagtc cgttcggttt cggttatttc cgataaattc ctgctgcttt
    14881 tcattcctag attcccactt tcgcgggaat gacgggattt cagggtaaag atggcggcag
    14941 ccggaggggt gtgaagagct gctttcgcag gaatgacgat atgcggattt gctgttttca
    15001 tccgctaccg atgccgtctg aagcctgttt ggcgtttcag acggcattgg gttttttgtg
    15061 catttgccgc aaaacggatc ggtgtctgct tgcggcaaga tgaaaacggc gcggaatgtt
    15121 ttgggaaacc aatgccgtct gaagggcttt cagacggcat ttttgtaccg tgccgtttaa
    15181 cgcgccagcg gttcggtgcg tttaatcagc cacgcttttg ccgcgccttc ggtcagcggc
    15241 tcgaggcggc ggcggacttc ggcgtggtag cggttgaccc attcgatttc gccgtcggtc
    15301 atcagggcgg tgtccatcag gcgtgtatcg atggggcaga gggtcagggt ttcaaaacag
    15361 aggaagctgc cgaactcggt ttcctgcggg tcggcgacgg cttggttggc ggcgaggttt
    15421 tcaatgcgga tgccccattt tcccggacgg tagaggccgg gttcgatgga ggtaaccatg
    15481 ccttttttca tggcggtttc gggcgtggcg ggggcggcga aggcgatgcg ctgcgggcct
    15541 tcgtggacgt tgaggaaata gcctacgccg tggccggtgc cgtgtccgta gtcgcattgc
    15601 gcctgccaca ggggtttgcg gcaaatcgcg tcaatcagcg gcgaggggat gttttcgggg
    15661 aacacggctt cggcaagcgc gatatgggct ttgagaacga gcgtgttgtc gcgtttttgt
    15721 tctgctgtgg gcgtgccgac ggggacgacg cgggtgatgt cggtcgtgcc gcctttgtat
    15781 tgcgcgccgg agtcgatgag cagcagaccg ttgccgctga tggtgctgtg gctttcgggt
    15841 gttgcgctgt aatgcggcag ggcgccgttg gcgttgaagc ctgcgatggt gtcgaaactc
    15901 aaggacacaa aacccgggcg cgcgctgcgg tggcggtaaa gcatcgtatc cacgtcgatt
    15961 tcggtcaggc tgccgccttt gccgatgatg tcttcaaact cggcgaagaa gccgcacaac
    16021 gccgcgccgt cttgttccat cgcttcgcgg atgcgggcga tgtcggcttc ggatttgacg
    16081 gatttgaaga aggtggacgg gttgatgcct tcaatcaggc gcgcgctttc gggcaggcgc
    16141 acgagcgtac tgacggcggt tttgttcggc tcgatgagca gcgcgccgcc gatttgcgcg
    16201 agtttgtcgg caacttgggc gtaaggctcg accgtgatac ctgcggtttg cagcgcggcg
    16261 gcggcttcgg cgttcaggcg gcattgttcg gtaaacagga cggcactgtc tttgccgatc
    16321 agcaggaagg acacgaaaac agggttgaaa ggcacgtcgc tgccgcgcag gttggtcagc
    16381 caagcgatgt cgtcaagcga ggaaaccaag tggtaatccg cgcctttttc cgccataact
    16441 gcgcgcacgc gggcgagttt ctcggcggcg gtttcggaga cgtaggcgtg gtcgtggatg
    16501 aacacggttt cggcggggat agcgggacgg cttgtccaca cttggtcaag caggtcgtcg
    16561 gggtgttgga tgcggatgtt tttggcggcg agcgattgcg ccaaagtgcg tttgccggtg
    16621 agcgacacca tatcggaagg gatgccgacg gcagcgtttt cgggcaggtt tgccgcgagc
    16681 cattcgttgt acggcggcac ttgcccgctt ttttgcagct caatgccgct gccggaaagc
    16741 tgtttggcgg cttgttccca atagcggctg tccacccata cgcccgcttc attggcggta
    16801 acgacgaatg tgccgaccga gccggtaaag cctgacaatt cgcgccgcgc ctgccaatgc
    16861 tcgggcaggt attcggatag gtgggggtcg gcggaaggga tgacgagtgc gtccaagcct
    16921 tgcgccttca tggcttcgcg caatgcggac aggtaattcg atacggtatt cataacagtt
    16981 cctccaatcg ggtttcgcgg cttcagacgg catggacgga aatctgaaat gccgtctgaa
    17041 aaacataacc atattatcaa atcgccagcc tgctgcaaaa cggacgggct gatcggatgg
    17101 tgcgtgtttg cacgaaaaaa agcgatgtaa ggttgtttgt tgtaaaattt caggcataaa
    17161 atgtcgtttc atgccttctt ttggaagtaa tacggaaaat atagtcaggt ttcagcatga
    17221 agttctaata gtgtgtgttg gcaggtattt ggattacgtt acaatacgcg ctttcatcaa
    17281 aatttcccta ccgggcggta tcctgttttt gaagacggta ggatttgggt ttgtttgggg
    17341 tcaatcccgc ccaaattgga gcaatagata gggctgtgtc cgaatatgcg gctctgtatg
    17401 ttgaaacaca gttaaaaata cggaagactt tatgtccgaa caacatattt cgacttggaa
    17461 aagcaagatc aacgcattgg gaccggggat tatgatggct tcggcggctg tcggcggttc
    17521 gcacctgatt gcctcgacgc aggcgggcgc gctttacggc tggcagctcg cgctcatcat
    17581 catcctgacc aacctcttca aatacccgtt tttccgcttc agcgcgcatt acactctgga
    17641 cacgggcaag agtctgattg aaggttatgc cgagaaaagc cgtgtttatt tgtgggtttt
    17701 tttgattttg tgcatcattt ccgccaccat taacgcgggc gcggtcgcca ttgtaaccgc
    17761 cgccatcgtc aaaatggcgg caccctcgct gatgtttgat gccggcacgg ttgccgcctt
    17821 gattatggca tcctgcctga ttattgtggt aagcggacgc tatcgcgctt tggatcgtgt
    17881 ttccaaaatc atcattgtta ctttgagcat cgccacgctt gccgccgccg gcatcgctat
    17941 gtcgcgcggt atgcagatgc agcccgattt tatcgagccg acaccgtgga cgcttgccgg
    18001 tttgggcttc ctgatcgcgc tgatgggctg gatgcccgcg ccgattgaga tttccgccat
    18061 caattctttg tgggtaaccg aaaaacaacg catccggcct tccggctacc gcgacggcat
    18121 tttcgatttc aacgtcggtt atatcgccag tgcggttttg gctttggttt tccttgcact
    18181 gggcgcgttt gtgcaatacg gcaacggcga agcagtgcag atggcgggcg gcaaatatat
    18241 cgggcaattg attaatatgt atgccgtaac catcggcggc tggtcgcgcc cgctggtggc
    18301 gtttatcgcg tttgcctgta tgtacggcac gacgattacc gttgtggacg gctacgcccg
    18361 cgccattgcc gagcccgtgc gcctgctgcg cggcagggaa aaacccggca acgccgaact
    18421 ctttgcctgg aatatttggg tggcgggcag cggtttggcg gtgattttct ggttcgacgg
    18481 cgtaatggcg aatctgctca aatttgcgat gattgccgcc tttgtgtccg cccctgtgtt
    18541 cgcctggctc aactaccgcc tcgtcaaagg ggacaaacgc cacaggctta ccgccggtat
    18601 gaacgccctt gccattgtcg gcctgctcta cctggccggg tttgccgttt tgttcctgct
    18661 gaacctgacg gggcttttgg catagtttcc aaaacacgga tgccgtctgc aagattttca
    18721 gacggcatcg ccctttcttg gaaaaaaaca ccgacatcgc gtaaaatacg cgcaaatttt
    18781 gtgattcggt caggtcgtct gaaacagact gcgcctgatt tattttttcg gaaaacccta
    18841 tgagcgaaca aaaccatccg caaaccgagc cgcagttgga cgaaaaccaa atcatcgccc
    18901 tgcgccgcga aaaactgaac aacatccgcc aacagcgcaa cgcttacccc aacgatttca
    18961 agcgcgacag ctttgccgcc gatttgcagg cgcaatacgg cgaaatcggc aaagaagaac
    19021 tcgacccgca ggccgttccc gttaaaattg ccggccgcat gatgctcaaa cgtcaaatgg
    19081 gcaaagccag cttcgccacc attcaagacg ttaccggtca aatccagctt tatctgaaca
    19141 acaaaggcgt gagccaagaa gttttggacg acttcaacca ctgggacttg ggcgacatcg
    19201 tcggtgcgga aggcactttg ttcaaaacca accacggcga actaaccgtg cgtgtgtccg
    19261 acatccgcct gctgtccaaa tccctgcgtc cgctgcccga caaacacaaa ggcttgagcg
    19321 atcaggaaac caaataccgc caacgctacg tcgatttgat tgccaatgaa gaatcgcgca
    19381 atacctttat caaacgcagc caaatcatcc aatctgtgcg taattttatg gtgggcgagc
    19441 attatctcga agtcgaaacc ccgatgatgc acccgattcc cggcggcgcg acagcgaaac
    19501 ccttcgttac ccaccacaat gccttggaca ttccgctcta cctgcgcatc gcgcccgagc
    19561 tgtatctgaa acgcttggtg gtcggcggct tggaacgcgt gtttgaaatc aaccgcagct
    19621 tccgtaacga gggcatgtcc gtgcgccaca accccgaatt tacgatgatt gaattttacg
    19681 aagccttctc cgactacgaa cgtatgatgc agatggcgga agacatcatc cgcaacgcat
    19741 cgcgcacggt aaacggcacg gcaaacatca cttacaacgg caaagaagtc gatttggaaa
    19801 gcccgttcga acgtctgacc atcctcgaag ccatcaaaaa atacaatccg cactacaccg
    19861 acgagcagtt gaacgatgcg gaatggctga aaaaagaaat cgtcaaacac ggcgaaagcc
    19921 tgccgccgtc cccgggcatc ggcagcctgc aactcgccct gtttgaaggt tgcgccgagg
    19981 gcaaactgtg gaatccgacc tttattgtcg attacccggt cgaagtttcc ccgttggcgc
    20041 gcgcttcgga tacgaaacaa ggtctgaccg agcgcttcga gcttttcgtt gtcgggcgcg
    20101 aactggcaaa cggctattcc gagttgaacg accccgaaga ccaagccgaa cgcttcaaat
    20161 cgcaagtggc gcaaaaagat gcgggcgacg acgaagccat gcactacgat gccgactaca
    20221 tccgcgcgat ggaattcggt ttgccgccga ccggcggttg cggcatcggc atcgaccgct
    20281 tagtgatgct gctgaccgat ttgcaaacca tccgcgatgt gattctgttt ccgcagatgc
    20341 gtcccgagta at
//
LOCUS       DAXFWM010000037        20316 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000037 DAXFWM010000000
VERSION     DAXFWM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20316
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.037"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(133..1554)
                     /locus_tag="ACFI8B_001253"
     CDS             complement(133..1554)
                     /locus_tag="ACFI8B_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC3834478.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1913..2272)
                     /locus_tag="ACFI8B_001254"
     CDS             complement(1913..2272)
                     /locus_tag="ACFI8B_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC3834479.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2269..2874)
                     /locus_tag="ACFI8B_001255"
     CDS             complement(2269..2874)
                     /locus_tag="ACFI8B_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HFC3834480.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2921..3403)
                     /locus_tag="ACFI8B_001256"
     CDS             complement(2921..3403)
                     /locus_tag="ACFI8B_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC3834481.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3607..3704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3915..5015
                     /gene="gcvT"
                     /locus_tag="ACFI8B_001257"
     CDS             3915..5015
                     /gene="gcvT"
                     /locus_tag="ACFI8B_001257"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC3834482.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKVLQTA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     gene            5162..5701
                     /locus_tag="ACFI8B_001258"
     CDS             5162..5701
                     /locus_tag="ACFI8B_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834483.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5753..6139
                     /gene="gcvH"
                     /locus_tag="ACFI8B_001259"
     CDS             5753..6139
                     /gene="gcvH"
                     /locus_tag="ACFI8B_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC3834484.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6292..7539
                     /gene="hemA"
                     /locus_tag="ACFI8B_001260"
     CDS             6292..7539
                     /gene="hemA"
                     /locus_tag="ACFI8B_001260"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC3834485.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7849..10091
                     /locus_tag="ACFI8B_001261"
                     /pseudo
     CDS             7849..10091
                     /locus_tag="ACFI8B_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449380.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10220..12187
                     /gene="nosZ"
                     /locus_tag="ACFI8B_001262"
                     /pseudo
     CDS             10220..12187
                     /gene="nosZ"
                     /locus_tag="ACFI8B_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12472..12627
                     /locus_tag="ACFI8B_001263"
                     /pseudo
     CDS             12472..12627
                     /locus_tag="ACFI8B_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12624..13715
                     /locus_tag="ACFI8B_001264"
                     /pseudo
     CDS             12624..13715
                     /locus_tag="ACFI8B_001264"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13773..14669
                     /locus_tag="ACFI8B_001265"
     CDS             13773..14669
                     /locus_tag="ACFI8B_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3834486.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14666..15496
                     /locus_tag="ACFI8B_001266"
     CDS             14666..15496
                     /locus_tag="ACFI8B_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3834487.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15493..15987
                     /locus_tag="ACFI8B_001267"
     CDS             15493..15987
                     /locus_tag="ACFI8B_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC3834488.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16121..17782)
                     /locus_tag="ACFI8B_001268"
     CDS             complement(16121..17782)
                     /locus_tag="ACFI8B_001268"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC3834489.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(17912..18493)
                     /locus_tag="ACFI8B_001269"
     CDS             complement(17912..18493)
                     /locus_tag="ACFI8B_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3834490.1"
                     /translation="MFNDNPVVYGKIKLQSWKARRDFNIVKQDLDFSCGAASVATLLN
                     NFYGQTLTEEEVLEKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIV
                     YLKYRKDDHFSVLRGVDGNTVLLADPSPGHVSMSRAQFLEAWQTREGNLAGKILAVVP
                     KGRDAGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
     gene            18560..19212
                     /locus_tag="ACFI8B_001270"
                     /pseudo
     CDS             18560..19212
                     /locus_tag="ACFI8B_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            19352..20293
                     /gene="mafA"
                     /locus_tag="ACFI8B_001271"
     CDS             19352..20293
                     /gene="mafA"
                     /locus_tag="ACFI8B_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC3834491.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSATRYSYPA
                     YDTTATTKSDALSGVTTSTSVLNAPAAALTKNNGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
BASE COUNT         4955 a         5411 c         5392 g         4558 t
ORIGIN      
        1 ttaacttcgg cacaccgccc cggcagctaa aaatcctgcg ggatcggtgt ggaatttagg
       61 gataatctag tacagcccct ttgattatgc cggaatttaa aatgccgtct gaatgttcag
      121 acggcatagc gtttacagta gtttgaaaac gaaaaagacg agggtatgta cgatgaagac
      181 gggtgtcagg aaggcgaccg accacatcat atagccgaag aaagtcggca tcggtacgcc
      241 gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg ttgccgatgt aggtcagtgc
      301 gcccatgaat accgaaccca tagaaaccgc cagcagcgaa tgaaacaggg gacccgtcat
      361 taaggcttgg gcatcgccgc ccgccatatt gaaaaacacg agataagtgg gcgcgttatc
      421 caagaatgcc gacaatatgc cgctcatcca gaaatacatc gtattaatcg gatgacctgc
      481 cgtatcgtga accagcgata ccaccccgcc cagcgcgcct gcctcgcctg ctttcagaat
      541 gctcaggacg gggaagatgg tgatgaagat gccgaggaag agtttgccca cttcggcgat
      601 gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg ggcgtgattg ccatagatac
      661 ggcggtcaat gtaatcagga tgacatcgcg gacaaggttt tgcagggcgt aacggctgcc
      721 gaggatttca aatcccgggt gttcgggttt ccaaaggccg gacattagaa ccgcgccgac
      781 cacgcccgaa agcaggagga agttccattt gccgaagatg gcgatttttt cgggtttttc
      841 ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg aagaaacggt tgtcgatgaa
      901 atagaaggcg gtcaacagga cagcggtgct aatcaggacg ggggcgaaca tatgtttgac
      961 cgtccacatg aaatctacgc ctttgaggaa gccgaggaag agtggggggt cgcccaaagg
     1021 ggtcaggccg ccgccgatgt ttgcaaccag gaaaatgaag aagatgacga tgtgcacgcg
     1081 gcgggtacgg tcttggttgg ctttcagcag cggacgaatc atcagcattg ccgcgccggt
     1141 cgttcccatg atagaggcaa gtgccgtacc gacggcaagc agggcggtgt tgagcttggg
     1201 tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa atggtgtaca gggcaagcag
     1261 cagcaggatg aaggggatat attcttcaac gagtgcgtgt gcgacggtat ggataccggc
     1321 ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc aatgtccaaa aggcggtgat
     1381 tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag ggacccaatg cgatagacag
     1441 caaaatcagg gcaaagggca agccccacag caggtttagg tttgcgccgt ccgaatctgc
     1501 ggcgtaaacc gatgccggga aaagcattag tgaaaacagg ggtaggtggc gcatcgtgtt
     1561 tcctcgattc aagcactgcc ttgcgcggcg cgcgggagtg atacaggctt cgtgccgccc
     1621 ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa agttttccga ttgattgtaa
     1681 agtaaataat caggataaac cagtttccca aaccggaagg cggcgggaag gcggattgcc
     1741 gtgcttggga atatatgttc tttttgataa ataattttat ttaaaacaaa taaatatatg
     1801 tctttaataa ataatctgtc gaaaacgaaa aataaattta ttttaacata tatttgcaat
     1861 gaaacaggtt tgccccaccc gtttgtttgc ccttatccct ttcagacggc attcaagatt
     1921 cgggtctgcg ccacatccat atggcgacaa gggaacaaaa aaccgatgaa accgccccga
     1981 cccaccagcg ttggggaaac tgccaaaaca tcatcaggca ggatgcggtc atcatgctga
     2041 tggcgaaaat cttggctttg cgcggcactg cgccgttttg ttcccagtta tgaaccatcg
     2101 ggccgaaata gcggtgccgg tgcagccagc ggtgaaagcg cggggatgcc tttgcccagc
     2161 aggcggcgga gagtagtacg aacggcgtgg tcggcaacag cggcaaaaaa atgccgatta
     2221 tccccaacag cagggagatg ccgccgcagg caattaaaag ataacggatc attttgaaat
     2281 atttttctta ttgtgcggat aagggcagga tgtgataccg agttttgccc agccttcatg
     2341 tcccattttt tccagcaggg cgatattgcg ttcgaatatt gccgaagcgt cgggaaaggc
     2401 ttgtgcggct ttggcaatgc tgtcttcgcg gatgaggtgc agcgtcggat agggagaacg
     2461 gttggtgtag ttgccgatgc cgtctgaatc cgtgccttca aattggaaat agggatgaaa
     2521 cggggcgatt tggacgatgc cttccaagcc gttttcgaca acggcggcat cggcaatgtc
     2581 gagcatatcg ttgaatacgt cgaaatcggg gaatagggtc gggtgaacca gcagggtggt
     2641 ttccagttcg gtggcgggtg tattgcccag tcgctgcagt tcttcgtcca agtcttccaa
     2701 aaaaccgtca aggtgtttgg cttcgctgat cgcgatgcgg acaaggtttt taacgtgggg
     2761 ggctttggca aagggacaca ggttcagacc gatgacggct ttttccaacc attgtccggt
     2821 gtgttcggca acagcatctt tattttcgga agtatcgata ttcattattg tcatgtaaat
     2881 gtgtttgcag agtgcacgtg cgggaaaatc gggaagggca ctattccttc agcaggtggt
     2941 tgagcggcag ggaggtggtg tgtttgattt cttttaaaac aaagctcgat tgcgcatcct
     3001 gtacgccgtg gtgggataaa agcgtatcca aaacaaaatg ggaaaacgcg ttcatatcgg
     3061 taaaaaatgc ctgaagcagg tagtcggtct cgccggttag ggcgaagcag ctcaagactt
     3121 ccggccattt tcgaaccgat gcggcaaaat cttcccgcgc gtcttttgct ttgcggatgg
     3181 aaacgcggat aaatgcctga agacctaagt tgacggattc cggagacagc agcgcggcat
     3241 attggcggac gatgccggca tcttccaact gcttcagacg gcgcaggcac ggagaaggcg
     3301 aaagtgcgac acgttcggac agttcgacat tggtcagcct gccgttttcc tggaggacct
     3361 gtaagatttt aatatcggtt ttgtctaaag tgagttgggg catatttgcg ttccgtttta
     3421 aggaattcgg attgtctgtc cgtatgtttg cggcaatccg catagatgga gaccatatta
     3481 acatataaaa agttataccg tcatccggga caaattttgt tttcggaaaa tcatgtgaaa
     3541 acagaggcgg tcggtttgca tctcttttaa gacggcttgc ccaaaccgcc gattcaagac
     3601 ataatcggga aatgtgcagg agagtgttac acccaactac aatgtaacca ccgaaggcgc
     3661 agacaccctt aaatcgctca ggtatcaggg actgcacatt gaaacaaaca atctggagag
     3721 cggcgttaga ataacgtcca ccgaagggga gaaggccgtc tgaaccacca ttcagacaac
     3781 cgcgcaaagc agtgagcaga ctggtttgcc atcatgcgga tacggccgaa aatctcaggt
     3841 tcaaggacag atagggtcat ccgcgcacag gtgcgcgggc ggcatctgaa caaaaaatcc
     3901 ggagaaactt gagaatgact gctctgaaaa ccaccccgtt tcatcaagcc catcaagatg
     3961 caggcgcgaa gccggtagat tttgccggct gggaactgcc catccattat ggttcgcaaa
     4021 tcgccgaaca cgaagccgtg cgcaccgacg ccggtatgtt tgacgtatcc catatgctcg
     4081 ttaccgacgt cgtcggagcg aacgccaaag cctttttccg caaattgatt gccaacgacg
     4141 ttgccaaact cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4201 gcgtgattga cgacttgatc gtttaccgca ccaacgaggc cgaaacccaa taccgcatcg
     4261 tgtccaacgg tgcgacccgt gaaaaagaca cggcacaatt ccacaaagtc ggacaagagt
     4321 tcggcgttgc cttcaatccg cgctacgacc tcggtatgct tgccgtacaa ggtcctaaag
     4381 ccattgaaaa actcctgacc gtcaaacccg aatgggcgga tgtgattcac ggcctcaaac
     4441 cgttccaagg cgcggatttg ggcaacgact ggtttgtcgc ccgtacaggc tacaccggcg
     4501 aagacggcgt cgaagtgatt ctgccggata ccgaagccgt cgcattcttc aaagtcctgc
     4561 aaacagcagg cgtacagcct tgcggcctcg gcgcgcgcga caccctgcgc atggaagccg
     4621 gtatgaacct ctacggcaac gatatggacg acgacaccag cccgctcgaa gcaggcatgg
     4681 gccggacggt tgatttgaaa gacgaaagcc gcgattttgt cggtaaagcc gccttgttgg
     4741 cattgaaaga aaaaggcgtt gccgtcaaac aagtcggttt gctgctcgaa aaaggcggca
     4801 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggaaa aggcgaaacc accagcggcg
     4861 tattctcgcc cagcctgaaa caatctatcg ccatcgcccg cgtaccgaaa gattttgacg
     4921 gcgataccgc caaagtcctg attcgcggca aagaagcgga cgtgcgcgta ctgaagctgc
     4981 cgttcgtccg cagcggtcaa aaacagtttg attaatatcg gttcagacgg catttgtttg
     5041 aattaagccg tctgaaagca ggttttaagg aattgttatt tcctatgaaa gcaaattttg
     5101 gcagtttatt gttgttattg atgttatcgg cttgcgcttc gtcgccggcg agcgcggatt
     5161 gatgtttttc gatacagaag gaaaatccca tactgcaata tttatgaaaa tcgttggcgg
     5221 caatggaaag attgccggcg aggtggtgat agacggggaa tatgctcttg atattgtccg
     5281 catgaatgat gatttgagta ttgccgtgca agataataag ggtagaagcc gtgcttccga
     5341 cctggaagca ttgtcgcaag cgaaatcatt taccctaatt gcgttcagcc gttatgcggg
     5401 cacagttgaa gtggataaag tatcggctgt tacaggcatt tgtgcagatt accgcagcaa
     5461 aggcctgaag gttgccgttg cagacaatat gaggcccaaa caagaaatcg ttgtattccg
     5521 tgcaagcggc gtaatggcaa gtaataagcg caagataaac gaagcatatt tggattattc
     5581 tgcatcctct aaactgcctt ccgcccaaaa gcaagaattg gaacaagata aaaacaatat
     5641 gattgccgtc cattacggac gcttggaaga attaatcgca cgaggtatct gcgtacgcta
     5701 agttgtgcag atgccgtctg aaaataaccc cctaccaatg gagaatcaaa ccatgagcaa
     5761 caacatccca acagaactga aatacgttgc cagccatgaa tggctgcgcc ttgaagaaga
     5821 cggtatcatt accgtcggta ttacccatca cgcgcaagag ctgttgggcg acatcgtgtt
     5881 tgtcgagctg cccgaagtcg gtgcgaacct cgccgccgaa gaacagtccg gtgtggttga
     5941 gtctgtaaaa gccgcatccg acgtgtatgc gccgattgcg ggcgaagtcg ttgccgtcaa
     6001 cgatgacttg ccaggcgcgc cggaaaccgc caacagcgac ccttacggcg caggctggtt
     6061 cttcaaaatc aaacctgcca atcctgccga ttacgacggt ctgctgactg ccgaacaata
     6121 cgcgggcgaa gtggattaaa ccgcccgttt gtccgacggc aaccgtcgga caaacggaaa
     6181 ctgcaccttc agacggcatt tctgcggtcg gaggtgcagt ttttttgtcc gtgttttaag
     6241 gatgcagtta ggctataata acggtctata tccatcttca ccgatttttt catgcaactt
     6301 accgctgtcg gactcaatca tcaaaccgca cctttaagca tacgggaaaa gctggcgttt
     6361 gccgccgccg ccctgccaga agccgtccgc aatcttgccc gaagcaatgc ggcaacggag
     6421 gcggtaatcc tttctacctg caaccgcacc gagctttact gcgtcggcga ttcggaagaa
     6481 atcatccgat ggcttgccga ttaccacagt ttgccgattg aagaaatccg tccgtatctg
     6541 tacacgctgg atatgcagga aaccgtgcgc cacgccttcc gcgttgcctg cggcttggat
     6601 tcgatggttt tgggcgagcc gcagattttg gggcagatta aagatgcggt gcgtgcggct
     6661 caagaacagg aaagtatggg ggcaaaactc aatgccctgt tccaaaaaac cttttccgtt
     6721 gctaaagaag tccgtaccga taccgctgtc ggcgaaaatt cggtttcgat ggcttccgcg
     6781 tccgtcaagt tggcggaaca gatttttccc gacatcggcg atttgaacgt attgtttatc
     6841 ggcgcaggcg aaatgattga gctggttgcc acttattttg ccgccaaaaa tccccggctg
     6901 atgacggttg ccaaccggac gctggcgcgt gcacaggagt tgtgcgacaa gctcggtgtt
     6961 aacgccgaac cgtgcctgct gtccgatctg cctgccattc tgcacgatta cgacgtggtg
     7021 gtttcttcaa cggcgagcca gcttccgata gtcggcaaag gcatggtcga acgcgcattg
     7081 aaacagcgtc agagtatgcc gttgttcatg cttgacttgg ccgtgccgcg cgatattgaa
     7141 gcggaagtcg gcgatttgaa cgatgcgtat ctttatacgg tggacgatat ggtcaacatc
     7201 gtccaaagcg gcaaggaggc aaggcagaag gccgccgccg ccgccgaaac gctggtgtcc
     7261 gagaaggttg ccgaatttgt caggcagcag cagggcaggc agagcgttcc gctgattaag
     7321 gccttgcggg acgagggcga gaaagcgcgc aagcaggtgt tggaaaatgc gatgaaacag
     7381 cttgccaaag gcgcaacggc ggaagaggtt ttggaacggc tgtccgtcca actgaccaac
     7441 aagctgctgc attcgccaac tcaaaccttg aataaggcgg gggaagaaga taaagatttg
     7501 gttcatgccg tcgcgcagat ttatcatttg gacaaataac ggtgcgccgg aaaaggatgc
     7561 cgtctgaagg ggtttcagac ggcatatttt tgtgccgcct gacaacatcg tgaaatccca
     7621 cattatatcg atgtaattac aaagtattat cctatatgcg gattggtttt aagatttgta
     7681 aatttgattt gcatcaaaaa tcgccgatag atgattcata taatatcaat attaaagagt
     7741 atcggtatat cggggatagc tatgtcctgt ttttcaatcc ggcgtatgtc tgcgtttcgg
     7801 gcgcggataa cggcgttttt taccgccttt gtctttttga cggcggcact gcccgcttat
     7861 gcggagcgtc tgcctgattt tctggcgaaa atacagcctt cggaaatttt tccgggtgcg
     7921 gatcgttacg gcaagccgga aggcaagcct atggttgccc gcgtttacaa aggcgatgag
     7981 cagctcggtt tggtttatat cacgaccgat gcggtcaata cgcgcggtta ttcgagcaaa
     8041 ccgatcgata cgctgatggc tttggcaaac gacggcacga tagccggggc gaaactggtc
     8101 gatcatcacg aaccgattat gctgatcggt atcccgcaat cgcgtgtcga taagttcatc
     8161 gacaaatata tcggtctgaa ttttattaaa aatccgccga ccccgagcgt ggcgccgggc
     8221 gacatcatca gcggtgcgac tgttacactg atggtggtta acgacagcat ccagcgttcg
     8281 tacaaggtca ttgccaacca ataccgtctg ggttcggaca aggcccttca gacggcatcc
     8341 gcttccgatg ttcgggaagc cgcgcctgcg tcagaaaccc gtccgcgccg tatggcaaat
     8401 cccgacaagc aggatatttt gtcttgggac gaacttttga aacaaaaggc cgtcggccat
     8461 ctgcatatca cgctcgatca aatcaacaaa ctgtttgaga aaggcggcaa ggccggcgtg
     8521 gccgatcacg ccgaacaggg cgatcctgac gataccttta ttgatttgta tgttgccttg
     8581 gtcagccagc cttccatcgg taaaagcctg ctgggtaagg acggctgggc gcatctgcaa
     8641 aaacggctga aacccgggca gcaggcggtt ttggttgccg gagagggccg ttattcttgg
     8701 aaaggttcgg gctatgtgcg cggcggtatt ttcgaccgta tcgagatgat tcagggggag
     8761 aacagcttcc gttttaccga tgcccaacac gaacgcgtcg tcgagctgtc tgccgccgat
     8821 gcgccgcgtt ttaaagaagt ttcttggttt accatccctg aaggcgtagc gtttgacggt
     8881 gcggagccgt ggcggctgta attgatggtt cagcgcgtct tgagtgtgaa cgataaagcc
     8941 ttcgttaccg ccgatttgga ttacgaactg ccccaagctt attacgtcga tgatcctaaa
     9001 gcgccgcccg tagaaatcag cgcgcctgtc gaagcagtac ctgccgcagc atccgatacc
     9061 gcttcagacg gcattgccga agatgcgtca gctgaaaacg gtgtatccaa ccaattatgg
     9121 aaacagattt ggaaagcgaa acaggggcag attgtcgtcg tcggcattgc gctgaccatc
     9181 ctgcttttgg tattcctgtt ccaagattgg attgtgcgtt acgaaaaatg gtacgaccgc
     9241 ttccgctttg ctttcctaac ttttacgctc ttctatatcg gctggtatgc acaggcgcag
     9301 ctttcggtcg tcaatacgct gacgctgttt tccgccatcc ttaccgaatt tcattgggaa
     9361 ttcttcctga tggatccgat tgtgttcatc ctgtggctgt tcacggctgc aaccatgctg
     9421 ctgtggaaca gggggacgtt ttgcggttgg ttgtgtcggt tcggctcatt acaggaattg
     9481 actaaccgca ttgccaaaaa actgggtgtg aaacagatta ccgtaccgca tatgctgcat
     9541 acccgtttga acgtcataaa atacctgatt ttattcggtt ttttggcaat ttcgctgtat
     9601 gatttgggta cggcggaaaa atttgccgaa gtcgaaccgt tcaaaactgc gattatcttg
     9661 aaattcatgt gcgattggtg gtttgtcgcc tttgccgtgg cacttttgat tgcggggctg
     9721 tttatcgaac gcttcttctg ccgttatctg tgtcctttgg gtgcgggcat cgcgctgccg
     9781 ggcagattcc gcgtgttcga ctggctgcgc cgctacaaaa tgtgcggcaa cccctgccaa
     9841 atctgtacgc acgaatgccc cgttcaggca atcgcgcccg agggcgacat ccatccgaac
     9901 gaatgtatcc agtgcctgca ctgccaagtg atgtaccacc acgatacgcg ctgtccgcaa
     9961 gttgtggcgg aaaataagaa aaacaaaaac aggctgctgc caaatcaggg gaattggaaa
    10021 atgtctctaa gcagccgcag gaacaggtcg tccgttttgt caaacccgaa acggcgcaat
    10081 ctgaaaaata agcatccggg ggaagcctga agccaaagtg tcaggcaggc agggtttccc
    10141 cggaaggata ataaaatttt tttcccatcg gcttgccgtc cgaaaaccgg tttgccgtat
    10201 tttatccaag gagtgttgta tgtcagacga aaaattagaa caaaacggct tgagccgtcg
    10261 ttcgttctta ggtacggccg ccgcttccgg tgcaggtatt gccggtgcgg gcctgttggg
    10321 tttggcgggt tgctctaaag acggcgaaca ggctgccgct aacgcttccg gcgcggctcc
    10381 cgtcgccaag gcgcaagggg aatccaaacc cggccaactg tcttccgaag tcggtccggg
    10441 cgaactcgat caatattacg gtttcctttc cggcggccag tccggcgaga tgcgcctgat
    10501 cggtctgcct tctatgcgcg aactgatgcg tatccccgtg ttcaatatgg acagcgcgac
    10561 cggttgggga cgcaccaatg agagcttgaa agtcctcaac ggcaatatta ccgaagaaac
    10621 ccgtaaattc ttaaaagacg gcggcctgcg ctgctacccc aacggcgact tgcaccaccc
    10681 gcacctgtcg tttaccgacc aaacttatga cggccgctat gcctatgcca acgacaaggc
    10741 aaacaaccgc gtctgccgcg tgcgtttgga tgtgatgaag gccgacaaaa tcatcgacat
    10801 ccctaatgat tcaggtattc acggtctgcg tccgcaacgt tatccgaaaa ccggttatgt
    10861 ttttgccaac ggcgaacaca ttacccctgt cagcggtgta ggcaaactgg atgatgcgaa
    10921 aacctggaat gcagtgtaca ccgccatcga cggcgaaacc atggagatcg catggcaagt
    10981 attggttgac ggtaacctgg acaacggcga tgccgactat caaggcaaat attcttttgc
    11041 cacctgctac aactccgagc gcgcgctgac cgtacaaggt gcgtcttcca acgagcagga
    11101 ctggtgcgtc gttttcgacc tgaaagccat cgaagaaggc atcaaagcgg gcgacttcaa
    11161 agaagttaac ggtgtgaaaa tgctggacgg ccgcgctgag gccaaatcca aatacacgcg
    11221 ttatatccct gtgccgaact ctcctcacgg ctgtaacgca agccctgacg gtaaatacat
    11281 catgcccaac ggtaaactgt ctccaaccgt taccgtattg gatgtcagca aactggacga
    11341 tttgttcgcc ggcaaaatca aagagcgcga tgtggtcgta gccgaaccgc aactgggtct
    11401 cggcccgtta cacactgcat tcgacggtcg cggcaatgct tatacgacat tgtttatcga
    11461 cagccaaatg gtgaaatgga atattgacga tgcgatcaaa gcctacaaag gcgataaaat
    11521 cgatccgatc aaacaaaaac tcgacgttca ctatcaaccg ggtcacaacc atacgaccat
    11581 gggcgaaacc aaagaagccg acggtcaatg gctggtgtcc ttgaacaaat tctccaaaga
    11641 ccgcttcttg aatgccggtc ctttgaaacc cgagtgcgac caattgatcg gcatctccgg
    11701 cgacgaaatg cgtctggtac acgacaaccc gacttttgcc gaaccgcacg acttgtgttt
    11761 ggttgccgcg tccaaactga acccgggcaa aacttgggac cgcaaagacc cgtggttcta
    11821 gcaggatgct ttggaacagg cgaaaaaaga cggtgtcgag ctggaaaaag ccgccaaagc
    11881 cgtacgcgaa ggcaacaaag tacgcgtgta tatgacggcc gtcgcgcctg cttacagtat
    11941 cccgcagttt gaagtgaacc aaggcgacga agtaaccgtt tacgttacca acgtcgaaac
    12001 catcgaggac ctgacccacg gcttcacttt ggaaggctgc ggcatcgcta tggaaatcgg
    12061 cccgcaagcc acttcttccg taaccttcaa agccgtccgc ccgggtgtgc actggtacta
    12121 ctgccaatgg ttctgccacg cattgcatat ggaaatgtcc ggtcagatga ttgttcatcc
    12181 caaataatgc ggcccgacgg ctgaatatgg agaatggcgt ctgaaggcaa caggtcttca
    12241 gacggcattt atagccaaac cgcttgccgt accgtttcag gcggtttcgc tataaaaaca
    12301 ggcacatttg aatgatgctt tattttgttc ggccataccg aacgaaacag agggacacta
    12361 tgcacacatc cgcgctccgt atatggctga aggccgtgct gatacttgcc gccggcagca
    12421 tttttcagac ggcatcggca gccgtcgtcc acgtttcccc gcaagacaac cttgccgaaa
    12481 tttttgcccg cgcccgggcg ggcgatacga tcaaactggc ttccggcgta tatcaaacca
    12541 aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttcc gcaaccatcg
    12601 aaggggacaa gagcgggcgt accatagccg tacacgcgcc ggacgtaacg ctccgcaacc
    12661 tgaccgttac ccgttccggt atgagcctgc cagcaatgga tgccggtatt tatctcgaaa
    12721 aagccgcccc gcgcgccctg attgaacaca acaatatttt tgataattcg ttcggcgtat
    12781 accttcatgg ttctgccgat gcgatggtgc gcgagaataa aatcgtcggc gatgcgacct
    12841 tgcgcgtgaa tgagcgcggc aatggcgtta ccgtttggaa cgcgcccggc gcgcaggtcg
    12901 tcggcaacga catttccaaa gggcgggacg gcattttttc caataccagc acgcacaaca
    12961 cctataaaaa caaccgcttc agcgacctgc gtttcgccgt ccactatatg tacaccaacg
    13021 acagcgaagt cagcggcaat atttgcgtgg gcaacaatat gggctatgtg ctgatgtttt
    13081 ccgaacggct caaagtgttc gacaatatcg ccgtcggcag ccgcgattag ggcatcatgc
    13141 tcaactatgt caactattcc gatattcacg acaatattat caacaaagcg ggcaagtgcg
    13201 tttttgccta caatgccaac tacgataaac tgtccgccaa tcattttgaa aactgccaaa
    13261 tcggcatgca ctttaccgcc gccatcgaag gcgcgtccct gcacgacaat tcctttatca
    13321 acaacggaag ccaggtcaaa tatgtcagta cgcgctttct cgactggagc gagggcggac
    13381 acggcaacta ctggagcgac aacagcccgt tcgatttgaa cggcgacggc ttcggagaca
    13441 gcgcgtaccg tcccgacggc atcatcgacc aaatcatctg gcgcgcgccc gtatcgcgcc
    13501 tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag tttcccgccg
    13561 ttctgcccgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc cccaaaattc
    13621 aaacccgtta tcaggcgatg aaggacgagt tgctcaaaga agccgaaacg cggcagtcgg
    13681 aacggggcag ggcggaaaac ggttctttga actagtctgc ttcagacggc acccggattc
    13741 aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc gaattgagga
    13801 aggtaaccaa acggttcggg gcgcaaaaag ccgtcagcca agtcgatttg gttttgaagg
    13861 caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc atcatgaagc
    13921 tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc gaacgtaccg
    13981 gcagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa accgttgcgc
    14041 tgcacccttc gctgaccggc atcgaaacgc tggattttta tgccaaactt aaaaaacagc
    14101 cgctcacgca gaaccggggg ctgcttgagc gcgtcggcat ttcccagacc gcacaccgcc
    14161 gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa gccctgctgg
    14221 gcgagcccaa agtcctgctg tttgacgaac cgacaaccgg ccccgaccct gcatcacgac
    14281 aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta ttgctcagta
    14341 cccacgccct tgccgagttg gacggacacg ccgaccgcat tgtcgtgatg aaaaacggcg
    14401 ttaaggttgc cgacggcagt atggacgaat tgcacgtcca aagcgggctg ccgctgaccg
    14461 tcaatatccg cctcaatgcg ccgcgcaccc tcagcagccg ctggcagccg ctttcagacg
    14521 gcatttccta ccgggcgcaa tgtcaggccg aagaacgtat ggaactgttg ggcgaattgg
    14581 gcagcctgtc cgaccttgcc tatttcgaca tccacacgcc gacgcttgac gaaatgtacg
    14641 cacggttctt gaaaagggag gacgtatgaa ccccgtttgg attattaccg gcaaagaagc
    14701 ccgcgacagc ctgcgtaacc gctgggtgct tgccgccgtc ctcctgcttg ccgcactcgc
    14761 cctttctttg ggtttcctcg gcagctcgcc caccggttcg gtcaaggtcg atccgctgac
    14821 cgttaccgtc gtcagcctgt cgagcctgtc tattttcctg attccgctga ttgcgatgct
    14881 gctttcttat gacgcactga tcggcgaaat cgaacgcggt acgatggcgt tgctgttgag
    14941 ttatcccatt tggcgcaacc aaatccttgc cggcaagttt gtcggacacc tcatcatcct
    15001 tgcccttgcc accacggcag gttacggatt ggcaggcatt acgctgcaac ttgccaacgg
    15061 cggtttcgac atcgccgttt ggaaaccctt tgcgctgttg attgccgcca gcgtgatttt
    15121 aggtgcggca tttctgtcta tgggctactt gattagtgca aaggtcaaag agcgggggac
    15181 ggcggccggt atttccatcg gcgtatggtt gtttttcgtc gtcatcttcg atatggcgct
    15241 tttgggtatt ctggttgccg actcgaaaca ggtcatcacc gcgcccgtcg ttgaaacggt
    15301 gctgctgttc aatcccaccg acatttaccg cctgctcaac ctgaccggtt acgaaaatac
    15361 ggctatgtat gcgggtatgg cgggtttgag cggacaaatc ggcctgaccg tccccgtttt
    15421 gctgaccgcg caggttttat gggttatcat tccgcttgtt ttggcagccg gaatttttag
    15481 aaagcgacga atatgaagaa aaccctgttg gcaattgttg ccgttttcgc cttaagtgcc
    15541 tgccggcagg cggaagaggc accgccgcct ttaccccggc agattagcga ccgttcggtc
    15601 ggacactatt gcagtatgaa cctgaccgaa cacaacggtc ccaaagccca gatttttttg
    15661 aacggcaaac ccgatcagcc cgtttggttc tccaccgtca agcagatgtt cggctatacc
    15721 aagctgcccg aagagcccaa aggcatccgc gtgatttacg ttaccgatat gggcaatgtt
    15781 accgattgga cgaatcctaa tgccgacacg gagtggatag atgcgaaaaa agccttttac
    15841 gtcatcgaca gcggctttat cggcggtatg ggcgcggaag acgcgctgcc gttcggcaac
    15901 aaggagcagg ctgaaaaatt tgcaaaggat aaaggcggca aggtcgtcgg ttttgacgat
    15961 atgcccgatg cttacatttt caagtaaatc caaacagaaa tgccgtctga acgttgaagc
    16021 caaggttcag acggcatttt ttgtcatagg ttcaaacggg gcaggttcga acaactgccc
    16081 cattccttat tcctgaccgg gcgtaacggc ggaatgaggt ttacatcccg ccgtaattcg
    16141 gtccgcttgc gccttcgggg caaatccaag tgatgttttg cgtcgggtct ttgatgtcgc
    16201 aggttttgca gtgcacgcag tttgccgcgt tgatttgcag gcgcggactg ccgttttctt
    16261 cgatgatttc atatacgccg gccggacaat agcgcgtttc gggagaggcg tattctttat
    16321 agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc tggttttctt
    16381 cgtgcgcgag attggcaagg aagacgctgc tcaagcggtc aaaggtcaac acaccgtcgg
    16441 gtttcggata atcaatcggc ttgcacacgg cggctttttt gagctgctcg ttgtctttgc
    16501 cgtgatgttt caaggtccac ggggctttgc ctctgaaaat catctggtcg atgccggtat
    16561 aaagcgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc gcggcataaa
    16621 gctcttgata cagccagctt tgttcaaaca gtttttgata gtttcccgct tctttgccgc
    16681 tgtcgaagct ttccacttct tcaaagtttt ccaacaaggg gaacacggct tcggcggcga
    16741 gcatggcgga tttcatcgcg gtatgaatgc ctttgatgcg cggcatattg aggaaccccg
    16801 ccgcatcgcc gattaaaacg ccccccttga acgagagctt cggcaggctt tgcaaaccgc
    16861 cttcaatcag cgaacgcgcg ccgtaagcaa tgcggcggcc gccttcaaag gttttgcgga
    16921 tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagatac gggttttgat
    16981 agtccaaacc gaccacgaag ccgacggcga ctttgttgtc gtcgaaatgg taaataaacg
    17041 caccgccgta ggttttgctg tccagcggcc agcctgcgct atgcaccacc aaaccgggct
    17101 gatgctgttc agacggcact tcccaaactt ctttgatgcc caagccgtaa gtttgcggct
    17161 ggctgttttg gtcgagttgg aaacgttcga tgatttgctt ggaaagcgaa cctcggcaac
    17221 cttcggcaaa cagggtttgc cgcgcccaaa gctccatgcc gggctggaaa ctgtcggtcg
    17281 gcccgccgtc tttgcctaca cccatattgc cggtcgcaat acctttgacc gagccgtctt
    17341 cgtgatacag cacttcggcg gcggcaaagc ccggatagat ttccacgccc atattttccg
    17401 cctgctccgc caaccagcgc acgacttcgc ctaagctgac gatgtagttg ccgtgattgt
    17461 cgaaattcgg ggtaataggc agattgaacg cttttttctc cgtcaggaac aacaccttgt
    17521 cctgagttac cgtgcgcgtc agcggcgcgc ctttttcttt ccagtcggga atcagctcat
    17581 tcagcgcaat cggatcgata accgcgcccg ccagcgaatg cgcccccgcc tccgaaccct
    17641 tctccgccac gcaaacgctg atttcgcgcc cgttctgttc ggcaagctgc ttgagtttga
    17701 tggcggcgga caaccccgac gggcctgcgc cgacaatcac gacatcgtat tgcatactgt
    17761 cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat tgcaaatccg
    17821 taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg aaaaccatat
    17881 tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat tttacctgtc
    17941 cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta tccccaccgg
    18001 catccctccc tttcggcacg accgccaaaa ttttgcctgc caaatttccc tcacgggttt
    18061 gccaagcctc caaaaactgc gccctgctca tcgaaacatg acccggcgac gggtcggcaa
    18121 gcaaaaccgt attgccatcc actccgcgca ataccgaaaa atggtcgtct ttgcggtatt
    18181 tcagatacac gatgacgggg attttcaact gcgcgagctg ctcgaaagac agggcatagc
    18241 ccttcgcctc aaaacccaaa tcgggcataa tgcgccgcat atcctcaaac gacgcgcgca
    18301 tctgttcctt acccagtttt tccaacactt cttcttccgt cagcgtttgc ccgtaaaaat
    18361 tgttcaaaag cgtcgccacc gaagccgccc cgcaggaaaa atccaaatcc tgctttacaa
    18421 tattgaaatc ccgccgcgct ttccaactct gcaatttgat ttttccgtaa acaacaggat
    18481 tatcgttaaa catcggtgca gcattcaaac gataagacaa gggtctgtac cagattagca
    18541 gatatgttac cctcgaaata tgaagataac acactgcaaa ttaaagaaag aagtacaaaa
    18601 agaaccgctc cgttcttttg taccggaagt taccgcccgt tctgccgccg atattttggg
    18661 tatccatccc gattcggcgg cactgtttta ccgtaaaatc cgcacggtta ccaaccatcg
    18721 tttggccttg gctgccgatg aggttttcgg ggccccgccg ggccgggcgg aagctgtttc
    18781 ggcgtacggc gtaaaggcag acgcggtcgc ggcgcggcag gaaaagcggt tgtcttcggc
    18841 attccgaaac gcaacgggcg ggcctatacc gttgccgcgg acaatgccga gcctgaaacg
    18901 ttgctccctg ccgtcaaaaa gaaaatcatg ccggacggta ttgtttatgc cgatagcccg
    18961 ggcagccgcg gcaagtcgga cgcgggcggt tttacccgtt gccgcatcaa ccgttccaag
    19021 gaatttgcag accgtcggaa ccacattaac ggcattggga acttttggaa tcaggcaaaa
    19081 cgcgccttgc ggaaatacaa cggaatcgac cgcaaacctt tcccgccgtt cttgagggaa
    19141 tgcgaatttc gatttaactc cggcacaccg ccccggcagc taaaaatcct gcgggatcgg
    19201 tgtggaattt agggataatc tagtacagcc acaaaaacaa tgccgtctga aagcccgccc
    19261 tgccgttcag acggcatttc cacccctttc gtttacaaac ccttaaaatc catttacact
    19321 taaacccgtt caaaacaaaa caaaccctgc catgaaaacc ctgctcctcc tcatccccct
    19381 cgtactcacc gcctgcggca cactgaccgg catacccgcc cacggcggcg gcaaacgctt
    19441 tgccgtcgaa caagaactcg tcgccgcatc gtcccgcgcc gccgtcaaag aaatggactt
    19501 gtccgccctg aaaggacgca aagccgccct ttacgtctcc gttatgggcg accaaggttc
    19561 gggcaacata agcggcggac gctactccat cgacgcactg atacgcggcg gctaccacaa
    19621 caaccccgac agcgccaccc gatacagcta ccccgcctat gacactaccg ccaccaccaa
    19681 atccgacgcg ctctccggcg taaccacttc cacatcggtc ttaaacgccc ccgccgccgc
    19741 cctgacgaaa aacaacggac gcaaaggcga acgctccgcc ggactgtccg tcaacggcac
    19801 gggcgactac cgcaacgaaa ccctgctcgc caacccccgc gacgtttcct tcctgaccaa
    19861 cctcatccaa accgtcttct acctgcgcgg catcgaagtc gtaccgcccg aatacgccga
    19921 caccgacgta ttcgtaaccg tcgacgtatt cggcaccgtc cgcagccgta ccgaactgca
    19981 cctctacaac gccgaaaccc ttaaagccca aaccaagctc gaatatttcg ccgtcgaccg
    20041 cgacagccgg aaactgctga ttgcccctaa aaccgccgcc tacgaatccc aataccaaga
    20101 acaatacgcc ctctggatgg gaccttacag cgtcggcaaa accgtcaaag cctcagaccg
    20161 cctgatggtc gatttctccg acatcacccc ctacggcgac acaaccgccc aaaaccgtcc
    20221 cgacttcaaa caaaacaacg gtaaaaaccc cgatgtcggc aacgaagtca tccgccgccg
    20281 caaaggagga taaaccgtga aaccgctgcg cagact
//
LOCUS       DAXFWM010000038        20315 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000038 DAXFWM010000000
VERSION     DAXFWM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20315
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.038"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            17..663
                     /locus_tag="ACFI8B_001272"
                     /pseudo
     CDS             17..663
                     /locus_tag="ACFI8B_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(735..2480)
                     /gene="recD"
                     /locus_tag="ACFI8B_001273"
     CDS             complement(735..2480)
                     /gene="recD"
                     /locus_tag="ACFI8B_001273"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC3834492.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(2547..3242)
                     /gene="lolD"
                     /locus_tag="ACFI8B_001274"
     CDS             complement(2547..3242)
                     /gene="lolD"
                     /locus_tag="ACFI8B_001274"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC3834493.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3235..4482)
                     /locus_tag="ACFI8B_001275"
     CDS             complement(3235..4482)
                     /locus_tag="ACFI8B_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC3834494.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4708..4986
                     /locus_tag="ACFI8B_001276"
     CDS             4708..4986
                     /locus_tag="ACFI8B_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834495.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5042..5659)
                     /gene="recR"
                     /locus_tag="ACFI8B_001277"
     CDS             complement(5042..5659)
                     /gene="recR"
                     /locus_tag="ACFI8B_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC3834496.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5726..7264)
                     /locus_tag="ACFI8B_001278"
     CDS             complement(5726..7264)
                     /locus_tag="ACFI8B_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC3834497.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7313..7687)
                     /locus_tag="ACFI8B_001279"
     CDS             complement(7313..7687)
                     /locus_tag="ACFI8B_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC3834498.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7837..9465
                     /locus_tag="ACFI8B_001280"
     CDS             7837..9465
                     /locus_tag="ACFI8B_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC3834499.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9544..10776
                     /locus_tag="ACFI8B_001281"
     CDS             9544..10776
                     /locus_tag="ACFI8B_001281"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC3834500.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENDEADALVAERVWQELAKGLMEKNPRKMIEMLRECGVLKVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11195..11503
                     /locus_tag="ACFI8B_001282"
     CDS             11195..11503
                     /locus_tag="ACFI8B_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834501.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11585..12616
                     /gene="ruvB"
                     /locus_tag="ACFI8B_001283"
     CDS             11585..12616
                     /gene="ruvB"
                     /locus_tag="ACFI8B_001283"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC3834502.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMDEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12861..13544)
                     /gene="rpe"
                     /locus_tag="ACFI8B_001284"
     CDS             complement(12861..13544)
                     /gene="rpe"
                     /locus_tag="ACFI8B_001284"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC3834503.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13690..14124
                     /locus_tag="ACFI8B_001285"
     CDS             13690..14124
                     /locus_tag="ACFI8B_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC3834504.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14137..15000)
                     /locus_tag="ACFI8B_001286"
     CDS             complement(14137..15000)
                     /locus_tag="ACFI8B_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3834505.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLALLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15008..15625)
                     /locus_tag="ACFI8B_001287"
     CDS             complement(15008..15625)
                     /locus_tag="ACFI8B_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HFC3834506.1"
                     /translation="MFTGIVQGLGKLTTIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15788..16402)
                     /locus_tag="ACFI8B_001288"
     CDS             complement(15788..16402)
                     /locus_tag="ACFI8B_001288"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC3834507.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16609..18378
                     /locus_tag="ACFI8B_001289"
     CDS             16609..18378
                     /locus_tag="ACFI8B_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFC3834508.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGMQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTDSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18375..19031
                     /locus_tag="ACFI8B_001290"
     CDS             18375..19031
                     /locus_tag="ACFI8B_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC3834509.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19232..20236)
                     /locus_tag="ACFI8B_001291"
                     /pseudo
     CDS             complement(19232..20236)
                     /locus_tag="ACFI8B_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         4681 a         5863 c         5094 g         4677 t
ORIGIN      
        1 gttacgcaat atataagggg ttgccgttcc gcagttgggc ggcagcatac acgttccttt
       61 cggcgtattc tcgccgttgc gcggcgaata tctcgacctg cttgcccatg cgccgtcaac
      121 gggttttcag acggcattcg atatcgggac gggctcgggc gtacttgccg ccattttggc
      181 gaaacagggc attccttccg tcatcggcac ggataccaat ccgagggcgg ttgcctgcgc
      241 ccgtgccaat attgcccgtt tgggttttga aaaacaggtt gaaatccgcg aaaccgattt
      301 atttcccgaa gggtttgccg acctgattgt ctgcaatccg ccctggcttc ccgccaagcc
      361 gacttccgcc gtcgaatccg cgttatacga ccccgaatct gcgatgctgg ctgcgttttt
      421 gcgtgatgcg ccaaacatct gaatcccgac ggagaaatcc gtcttatcat ttccgacctc
      481 gccgtgcatt tggggctgcg gtctgccgac tttttggaaa aggcatttat ccgggcgggt
      541 ttgcgtgttg aggatgtttt gaaaaccaaa ccggttcaca aaaaagctgc cgattcgaac
      601 gatccgctgg cttttgcccg aaacagggaa actactttcc tgtgccgttt gaaaaaggca
      661 taaggggcgg tggcgtgctt ccgacggtag gcatcggaac gctcgcgccg ccggcacggc
      721 atcattcggg cggattattc ttgtgaaaat acccgctcaa gcatactgcc caacgccgtc
      781 tgacggactt tgacggtgtt taccgcctga caaaatgtct ttttgccgcc gaaaaatacg
      841 aatttttccc tggcgcgggt aatggcggta tataacagct ccttactcaa tccggacaat
      901 gcatcgtccc cttcgtccga aggtgcgtcg gaaggcggca gcagccagac ttcccgatat
      961 tccgaacctt ggcttttgtg gacggtcatg gcgaatgcgg gttcaaattc gggcaagcag
     1021 cttaccgcta cctttttaaa tccgtccgca tcggcaaaat aggcggcaag gctgccctgc
     1081 cgtccgacat cttccataat cagtccgatg tcgccgttga acagctcaag cgcgtagtcg
     1141 ttctgtctga tcataatcgg ctctccggcg aaataggcca aatgttccgg tatgttcatt
     1201 ttgcggcgta catagctgca ataggcttcg ttgaagtctt ccgcatcctg ccgccaagct
     1261 gccagaacca cgatatccga gatgcctgcg tatgcggctt cgatattgcc gtcttttacc
     1321 gcctgccaat aggctttgtg cgcccggtac aacctttcga cccgagcatt cggactgcat
     1381 tccgaatgtt ccagttcgtc cggaaaccgg tcaaacaatg cccacgcccc ttcatcgccc
     1441 gatacggcgg cacgggcaag gcagccgatg ccgctgttgt cgccgaagcg gtggctgaac
     1501 gacagatggg cggtgttttg cgccaacacg ggcggatttg cgctgacgct gaaaccgtgt
     1561 tccggaagga aaccggccag cctttggtgc gtttctccgt ccaaaacggt tttttgcgac
     1621 aaaaccgaca gcaccgcccc gattccgacg gacgggagct ggttttcatc gcccagcaga
     1681 atcacgcgcg cgccggtttt gaccgctttc aaaagttgca gcatcaatgc ggtatccaac
     1741 atggaggctt catcgacaat cagcacgtcg aacggcagcg gacggatatg gtcgaacgcc
     1801 gcctgcattt tgggcgggct cagcttaagc agtcggtgga cggtttgccc ttccagtttg
     1861 agcaaatggc ggcggacggc ctccggcgcg tcaaaaccgt tgattgcacg gtgcagcgcg
     1921 cgcgccatat gtgccgccgc tttgcccgtc ggtgcggcaa gcgcgatatg gggaagattt
     1981 tcgttttcac cgcaaatcag tgccagcagt ttggcaaccg ttgtcgtttt gcccgttccc
     2041 ggccctccgg taatcaccat aaaaaactgc aacagtgcca aggcggccgc atcgcgctgt
     2101 ccttcgcttc ccgcgccttg aaaccatttt gcgaggtttt gccgcgcgcc tgccgcgtcg
     2161 ggggcggacg tgccggctgc cgccaagcgt tttatttcgg cagccaaatc gtattccaac
     2221 tgccacatcc tgcccaaaaa cagccttctg ccttccaaaa tcaaaggcgc ggcggatgtt
     2281 ccgaccacgg gtgcgagtgc cgacagcgcg tcagcctcgt ccccgctcaa acggataaac
     2341 gaatgaccgt tttgcaatgc ctgaaacagg cgttcggtac agtttgcaag cacttcgttg
     2401 cccgaacccg catagcgttc caaaaaacgg attgccgccc ttgccgccgc ttgggcaaat
     2461 tcatctgtct gcagttccat cgtattcctt gtcgaaatgc cgtctgaagg cacggggctt
     2521 cagacggtgc gtgttgtttc ggctgtttag gcgtttgcgc cctgtctggg gtgcaggctg
     2581 ccgtctttca tgaccatcac gcgctcgaag cggccggcga gttcgtcgtc gtgcgttacg
     2641 accaccagcc ccgttcccaa ttccgttttc agttccagca tcatatccaa aacattcctt
     2701 gcgtttgccc ggtcgaggtt accggtcggt tcgtcggcaa gcaggcattt gggttgcgta
     2761 accaaggcgc gcgcaatggc ggcacgctgg cgttcgccgc cggacagttc gcccgcacgg
     2821 tgcgtcgaac ggtgtttcag tccgaccttg tcgagcatcg ccattgccgt ttctaccgcc
     2881 tctttacggc tttttttgcc gatcagaagc ggcatcatca cgttttccag tgccgaaaat
     2941 tcaggcagaa gatgatggaa ctggtacacg aaaccgagat ggcggttgcg caaatcgccc
     3001 aaacgccgct ggtttaaggc acgcaaatcc tcgcccatca gcagcaccct gccttcagac
     3061 ggcatatcca gcccgcccaa aatatgcagc agcgtcgatt tgccgctgcc cgaagagccg
     3121 atgatgccgg tgctttcccc cgtgcggatt tccaaatcca agccgtacag caccggaacg
     3181 tccaaaccgc cgtcacggta gcgtttgccc acgccttcgc atttcaaaat caaatcactc
     3241 ataacgcaaa gcctccgccg gttgggtttt tgacgcgcgc cagctcgggt acagtgtcgc
     3301 cacgaaagac agccccaagg aaatgcaggc aatcagggca acgtcgccca tatcgacatc
     3361 actgggcagg tagtcgataa aataaacctg cgaattgatg aggtggacac cgagcaggtt
     3421 ttcaaaaaac gccacgaccc tgccgacgtt ccaaccccaa agcacgccgc agaccacacc
     3481 cgccagcgtg ccgaaaaagc ctgaaaacgc gccctgcacc ataaaaatct tcatcacgcc
     3541 ggcaggggaa agacccaaag tccgcaaaat cgcaatgtcc gcctgctttt ccgtaaccgc
     3601 catcaccaag gaagagacaa ggttgaacgc cgccacggcg ataatcagcg tcaggataat
     3661 gaacatcatc cgtttttcca gttcgaccgc ttcaaaatag ctgcggttgc tgtacgtcca
     3721 atcgcgcacc caaaccgtgt ccctttgcgc ctccggaatc agcgttgccg tcaaggcggg
     3781 ggcgttttgc ggatcggcaa gcttcagccg cagccccgca acttccttat ccaagcggta
     3841 cagtacgcgc gcgtcttgga tatgcgtcat cgccaatgag ttgtccactt cgtaaacacc
     3901 cgtcttgacc aggccgacca cggtaaactg cttcaggcgc ggcacgactc cggcaggggt
     3961 aacgttgccc tccggcgtaa ttaccgtaac cttgccgccg acttccgccc ccaaagcctc
     4021 cgccaaaccg acgccgagga taatgtcaaa ctcgcccgga atcaggtctt caaacttgcc
     4081 tgccggcatt ttgtcgccgt attccaccac tttgcgctct tcagacggca aaatgccgcg
     4141 tatctgaacg cccctgattt cgcccgcgtt tgccagcaat gcctgattgg aaacataggg
     4201 tgcggcagcc aaaatgcctt tgcggttttc ggcaaaccga agcaggttgc gccaatccgt
     4261 atccgtattg tcgatatagc cgatttcggc gtgcggcgcg acattcagga gctgcccgcg
     4321 tatttctttc tgaaagccgt tcataaccga caagacgaca atcagcgcgg ttacgcccaa
     4381 ggcgattccg gcaatcgaaa ccatcgtgat aaacgacata aagccgttgc gctttttcgc
     4441 cctgagatac ctcaagccta tccaagcctc tagagaaaac ataacgctac cttaaaaatg
     4501 tctgcaaacg tgccgccccg gacggcggtt tgggggaggc ggcaaaagtt tattgtaccg
     4561 taaaaccccg gcagcgtccg aacggcaggc tgtgtaaata aagacgcggt atgtggcaaa
     4621 ttcggaaaaa caggacggaa aacttgcaca acgcgccgaa ctaccctatc cttctcttga
     4681 aacaaaacct tttctttaag gaaaacaatg aatatcagga aaatctccgc tttgtgtgct
     4741 gttgccgttt ttactgtttc gacagcctat gccaaagaaa tcaaaatcga tgccaacaac
     4801 acgccttatt ccgaagccga cgcgcaaaag ctggcggcaa cggcagtcgg tatgggcgtt
     4861 aaggaacctg tcagcctgaa cggcggcagc ggcagcatta ccgtttccgg cagcagcgcg
     4921 gcgcagtgcg tctttacggt cggcagcggg ggcactttgc agattcaagg gctcaactgc
     4981 aagtaaaccg cccgaaaaaa tgccgtctga aggcttcaga cggcattttg cattggcggc
     5041 gttacgcccc gccttcttta atcagacgcc gttcgtacac cgcctgcgcc agcgttcccg
     5101 catcgacata ttccaattcg ccgcccaatg ggatgccctg cgacagcctg ctgaccttgt
     5161 aaggcaggtt tttgaaaaac tcggacagga catacgctgt ggcattgcct tctgcggtaa
     5221 aggcggtcgc gataatgatt tcttcgattt ccccgccgtc cagccgttgc gccagcctgt
     5281 ccaatgcgat ggcggatacg tccattccca atgccgtatt gatttgcccc atcaggacga
     5341 aatacagccc gtcgtggcag tttgccgctt ctatattcga cacgtcggca ggcatatgca
     5401 ccaccatcag ccgccgcccg tcgcgtgttt catcggcaca aatatcgcac aatccgcctt
     5461 cgcaaaatgt attgcacctt gcgcaatggc gaacctgcct caatgccgtc tgtaaggcat
     5521 ccaccagctc ttcagcctct ttgcgctttt gctgcaacag atggtacgcc atccgctgtg
     5581 ccgatttcgg cccgacgttg ggcaatactt tcagcgcacc gatcaggcgt tggaatgcat
     5641 cctgtttttt ggaattcatc atactccgcc atatgggaaa acggtcggaa tattccgacc
     5701 gttatgttgt caacaaaagt gtcaattact gaccatcgcc gttgtcaacc gattgtgctc
     5761 ctttagtctg tttgattttt ccgttgaaat agcggatcag caggtcgaaa gtattggcag
     5821 actgctgttg cgccaaagcc tgtttcgcag gaggaagctg tgcggcaata tcctccggag
     5881 gcgtgactgc ctgcacctcg acaatcacgg gtgccggcag accggtcagt ctgacatagg
     5941 cgggtttgcc gtttgccggt tttgctttca gcagttccgc ataagcctcg ggcggcatgg
     6001 actgccttgc ctgctgcgcg cccaaaacgg acacttccga ccatttcacg tcaactgcct
     6061 tgccgccgtt cagttgggta agcacttctt ttgccttgtt ttcggcaagt ttggcggctt
     6121 cggtacggat ataggcctga cgcaccgcat ctttggcttc ttcaaacagt aggttttttt
     6181 cttcgcggac ttctttggcg cggacgaccc acgcggtttc gctgttgatg gtcagcactt
     6241 cggaattgtg ttttttcttc aatacgtcgt cgctgaatac ggcattgatt aggttttcgg
     6301 gcatgccgga catttgtgcg tcctgcctgc tcagccaagt ttcttgggtt tccactttca
     6361 aaccgctgtt tttggcggct tcggcaagcg aggagggatg attgaacgca tcgtcgccca
     6421 gcttttcttt tgccttgttg aagtcggcaa ccgccttttt cattttcaat tcgttttcga
     6481 cggcggcttt ttcctgctcg aaagaaggtt tggcttcatg tgccggcaaa cgcgccacgc
     6541 gctcttcaaa cgcatttttc acttccgttt cactgacggt ctgcttgtct gcaaaatcct
     6601 tcagattcaa ggcgacatat tccaatttga ccgcctgcgg cagcagatag tcttttttgt
     6661 tcgcattata aaatttctgc aaatcggctt cagacgcttt gacttgggcg atgaactcgt
     6721 cggggttgaa agtgtgcgaa cggatggtgc ggttgacctg cgtcagcctg atcagctgtt
     6781 ccgcctgcgc gtcgccgacc aatacgccgt tttggacgag gcttaccaaa ttctgcaagg
     6841 caaactgatc gcggatttct tcgacaaact ggtcttcaga catatggcgt tgcgacaggt
     6901 attgactcaa aagcgcgtga ctgaatttgc cgtttgcgtc gtggaaattg ggatcgtcca
     6961 caatcatctg cttgatttgt tcggaagaaa ccgaaatgcc catcagcttc gcgccctgtt
     7021 tcaggtaggc gcgttgcagc agggattgga acaccgcgtc gcgccaaggg ctgccgccgt
     7081 ccgcctgctc gttctgcatg gcgttgttga ttgagtgctc gctgattttt tcgtcgccca
     7141 cttggacgat gtagtcggcg cccggatggg aaaccgtgct gacgccgaag ccgacaaaag
     7201 ttaatgcaat caggcctaat aagacttggg cgggtgttct gtatttttcg atggaatgga
     7261 acatattttc agattgggaa tgggaaattc aagtcgggta ttgtaacggt ttttatccct
     7321 gtctgcacgg ggcttgccgg ttgaagatgc cgtcgtaggt ttcttcgctg aagccgacgt
     7381 aaaccctgct gccgcactcc aatacgggac gcttgatcag gctcggcatt tcggacatca
     7441 gtttgacggc ctccgccgtc gaggacaggg ctttttgctg tgtttcggca tcgagtttgc
     7501 gccagcttgt cccgcgtttg ttgaacaggg ttgccaaagg cacttgttcc agccacgagc
     7561 agatttccgc ttcagacggc atctgttttt tgaaatcccg aaacccaaac tccaagccgt
     7621 atccggcaag cctgtttttg gcttttttga ctgtgtcgca atttggaatg ccgtgaagga
     7681 ttatcatttg gaaacctttt gcctgaaata ataaaaccga tattttacta taagtgtctg
     7741 aaaatttgcc cgtctgtttc agacggcggg gcggttatgt tacaatccga aaattcgaaa
     7801 aatttaatct cttgttcaat aaaggtttta cccataatga tttctaccaa cggcattacc
     7861 atgcagttcg gcgcgaagcc gctgtttgaa aacgtatccg taaaattcgg cgaaggcaac
     7921 cgctacggct tgatcggcgc gaacggttcg ggcaaatcca ccttcatgaa aatcctcggc
     7981 ggcgatttgg aacagaccgc cggcgaagtg gcgattgaaa acggcgtgcg tttgggtaaa
     8041 ttgcgccaag accagtttgc ctacgaagac atgcgcgtgc tggacgtggt gatgatgggg
     8101 cataccgaaa tgtgggcggc gatgaccgag cgcgatgcga tttacgccaa tcccgaagcc
     8161 accgaagacg actacatgaa agccgccgaa ctggaagcca agttcgccga atacgacggc
     8221 tacaccgccg aagcgcgcgc cgccgaatta ttaagcggcg tgggcatttc cgaagatttg
     8281 cacaatgcga aaatggcgga agtcgccccg ggcttcaaac tgcgcgtatt gctggcgcag
     8341 gcgctgttct ccaagccgga tgtattgctc ttggacgaac cgaccaacaa cttggacatc
     8401 aataccatcc gctggctgga aggcgtgttg aaccaatacg actccaccat gatcatcatc
     8461 tcgcacgacc gccacttctt gaacgaagtt tgcacccaca tggcggattt ggactacaac
     8521 accatcacta tttatccggg caactacgac gactatatgc tcgcttccgc ccaatcgcgt
     8581 gagcgcgccc tgaaagacaa tgccaaagcc aaagagaaac tgcaagagct gcaagaattc
     8641 gttgcccgtt tttccgccaa taaatccaaa gcccgtcagg caaccagccg tctgaaacag
     8701 gccgacaaaa tcaaatcgga aatggtcgaa gtcaaacctt ctacccgtca aaacccgtat
     8761 atccgttttg aagccgatga aaaagccaag ctgcaccgtc aggctgtgga agttgaaaaa
     8821 ctggccaaac gctttgaaac ccagttgttt agaaacctca acttcatcct tgaagcaggc
     8881 caacgcctcg ccatcatcgg cccgaacggc gcaggtaaat ctaccttgct gaaactcttg
     8941 gccggcgcgt acaaccccga atattcagac ggcctgttgc cggacgaagg cagcatcaaa
     9001 tgggcggaaa aagccagtgt cggctactat ccgcaagacc atgaaaacga ctttgacgtc
     9061 gatatggact tgagcgaatg gatgcgccaa tggggtcggg aaggcgacga cgaacaagtc
     9121 atccgaggca ctttggggcg tttgcttttc ggcagcaacg acgtggtaaa aaaagtgaaa
     9181 gttctctccg gcggcgaaaa aggccgtatg ctttacggca aactgttgct gttgaaaccc
     9241 aatgtcttgg tcatggacga accgaccaac catatggaca tggaaagcat cgaatcgctg
     9301 aacatggcac tggacaaata caacggcacg ctgattttcg tctcccacga ccgtcagttc
     9361 gtttcctccc tggcaaccca aatcatcgaa ctggacggca aaggcggata tgaacactac
     9421 ttgggcgatt acgaaagcta tctcgagaaa aaaggcgtag cataagcgca cccctccggt
     9481 tgaaacaatg ccgtctgaag ccgcttcaga cggcattgtt gataacttta aaataggaag
     9541 catatgcaga cttacctcgt cggcggcgcc gtccgcgatt atcttttggg cctgcccgtc
     9601 aaagaccgcg attgggtggt tgtcggcgcg gacgcgcaaa ccatgttggc gcaaggtttc
     9661 cagccggtcg gcaaagattt tcctgtattc ctccatccca aaacgcacga agaatacgcc
     9721 ctagcccgca ccgaacgcaa aaccgccaag ggttacgccg gtttcagttt ccacgccgac
     9781 aaagacgtta cgctggaaca ggacttgatg cgccgcgacc tgaccatcaa cgcgatggcg
     9841 caagatgcgg acggcaagat tatcgaccct ttcggcgggc aacgggattt ggcggcaggc
     9901 attttgcgcc acgtctcgcc cgccttcgcc gaagatcccg tccgcatcct tcgtgccgcc
     9961 cgctttgccg cgcgttacgg ctttgaaatc gccgaagaaa ccataaagct gatgcggcag
    10021 atggtggaaa acgacgaagc ggacgcattg gttgccgaac gcgtctggca ggagttggcg
    10081 aaaggtttga tggaaaaaaa tccgcgcaaa atgattgaaa tgttgcgcga atgcggcgtg
    10141 ctcaaagtct tgctgcccga agtcgacgcc ctcttcggcg tgccgcaacg cgccgactac
    10201 catcccgaaa tcgacagcgg catccatacc ctgatgacgc tgcaacgcgc cgccgatatg
    10261 ggtttgagcc tgcccgaacg ctatgccgcc ctgctgcacg acttgggcaa agccaaaacg
    10321 ccgcccgaca tcctgccgcg ccaccacgga cacgacatca acggcgtaga acccgtgcgc
    10381 gaagtcaatc aacggctgcg cgcgccgagg cagtgtgccg agcttgccga attggtttgc
    10441 cgttggcaca ttattttcca ccaagtcgga cagcttaaaa gccaaaccat tctgaacgta
    10501 ttgaaaaaaa ccgatgcctt ccgccgtccc gagcgttttc agacggcatt gaacgtctgc
    10561 attgccgaca cgcaaggccg tctgaaccga gaacacacgc cctaccccca gcgcgcgcac
    10621 tggctcgccc tgctcgaagc cgccaatcag gcggattcgg gcaaaatcgc cgccgaatgc
    10681 cgcgcgcagg gaaaagcgca ctttatcgcc gaacaaatcg accgggcgcg gctggcgcaa
    10741 atcgccccgc tgcaaaaagc gtttcggggc gcgtaggaaa aagaaaacgc caaacgcggc
    10801 atcttttgag gttcgcccgc attgccgaaa cagcgtcgga gaaagggatg gatgacatcg
    10861 gtttattcgg ctgcctttta atattccctt gatgcaggcc tgattagtcg gacaaaaata
    10921 ccttttgttc aacggaaccg atttctatcc ggacggaaac agccggcttt ccccttgcaa
    10981 acggatggaa tcaagcgggt attcaaacac agccgtttat ttttattccg ccgccaagtt
    11041 atgggatagg gtttgcgtgt ttcgggcaga caagccgcgc aaccttagac gcagtccgaa
    11101 gttttgaagg cggcatccgg gccgggtttt gaaacagccg ccgccatatc cgctataatc
    11161 catccttcag ccattccgcc cccgacataa aatcatgacc ctgaaaaccg atttattgcc
    11221 taaaatcaac aacgaagatt atcaacgcct catcctcaaa catagcgcgg aattcagcgg
    11281 cggagaaacc cgcctgttga acgaaatcct cgaaaaattc aatttcgacg ttgttcaggc
    11341 gcaggcattg gcgcaggcgg taatgcagca agttcgcttc gatcccaacg cctaccacat
    11401 cgacagcgac gacgaagaca ccaccggcat ctgcccccac tgcatcaacc cgcctatgcc
    11461 gcccctgcgc gactatctcg tttggcgcga aacacgcgga taaaacgctt ttgaccgtta
    11521 tcttttcaat gccgtctgaa acgccgccga ccgttcggac ggcatacccg acaaagggaa
    11581 cactatgctg caaaccgaca acctgaccgc cgcgcaaccg caacgcatcg ttgccgccca
    11641 aaccgcctcc gcacaggaag aactgctcga acgcgccctc cgccccaaaa cgctggacga
    11701 ctacatcggg cagcataaag ccaaagagca gctcgccatc ttcatccaag ccgccaaaaa
    11761 acgcggcgaa gcactcgacc acgtcctgct tttcggcccg cccggactgg gcaaaaccac
    11821 ccttgcccac atcatcgcca aagagttggg cgtaaatttg cgccaaacca gcggccccgt
    11881 cctcgaacgc gccggcgacc tcgccgccct tttgaccaac cttgatccgc acgacgtatt
    11941 gtttatcgac gaaatccacc ggctaagccc cgtcgtcgag gaaatcctct atcccgcgct
    12001 cgaagactac cggctcgaca ttatgatagg cgaaggaccc gccgcacgtt ccgtcaaaat
    12061 cgacctgccg cccttcaccc tcgtcggcgc gaccacccgc gccggtatgc tgaccaaccc
    12121 gttgcgcgac cgcttcggca tcgtctcccg cctcgagttt tacgaaaacc gcgaccttac
    12181 caccatcgtc agccgttcgg cacaactgtt gcagctcgat atggacgaag aaggcgcgat
    12241 ggaagtcgcc aaacgcagcc gcggcacgcc ccgcatcgcc aaccgcctgt tgcgccgcgt
    12301 gcgcgatttc gccgacgtga aaaacaacgg cgtaatcgat gccgccgtcg ccgatgccgc
    12361 tttaagtatg ctggacgtgg acgcgcaggg gctggacgtg atggacagga aattcctcga
    12421 agccgttttg cacaaattcg gcggcggccc cgtcggtttg gacaacgttg ccgccgccat
    12481 cggcgaatct acggacacca tcgaagacgt tatcgaaccc tacctcatcc aacaaggctt
    12541 tttgcaacgc accccgcgcg gcaggatggc gaccgaacgc gcctacctgc atttcgggct
    12601 gcccgtcgaa aaataaacca tgccgtctga aaccgaaccg ggtttcagac ggcatggtta
    12661 tcagacgttt gcaccggcac cggtttcatc acttaacaga aaccttttgt ccggcaggac
    12721 agattctccc cgatgaaaat aactgtttcc ctggcaaacg gttgaaaccc gtctggctgc
    12781 cggttgaaaa ataacgcaat gctgtctgaa acagagctaa ttttcagacg gcatttctat
    12841 ttcaatcatt ggctcaaggt tcagcctgcc gctttttcca gttccgccct catcgcagcg
    12901 attaccgcct tgtaatccgg tttgccgaaa atcgccgaac cggcaacaaa ggtatccgca
    12961 ccggctcggg caacggcggc aatattgtca gttttgatgc cgccgtccac ttcaatggca
    13021 atacgtctgc cgcttttccc ttcatagcgg tcaagcatgg cgcgaacctg tctgattttt
    13081 tccaaagtgt gcggaatgaa gctctgaccg ccgaacccgg ggttgaccga catcagcaaa
    13141 accatatcca gcctgtccaa cacgttttcc aacacatata cgggcgttgc cggattcaac
    13201 accagccccg cctgacagcc catgtcccga atcaggctca agctgcggtc gatatggcgg
    13261 ctcgcttcgg gatggaaagt gataatcgat gctcctgctt tggcaaacga ttgaattaaa
    13321 tcgtcaacgg gttcgaccat cagatgcaca tcgatcggca cgcttgcata aggcttcaac
    13381 gccgcgcaga ccataggtcc gaaggtcagg ttcggcacat aatggttgtc catcacgtca
    13441 aaatggatca gatccgcacc cgccgcaatg acatttgcca cctcttctcc gaggcgggca
    13501 aagtctgccg acaaaatact gggtgcgata cggtaggcgg tcatattttt tccttcaatg
    13561 cctttatggg cggactcaac gcgggaaagc ctcgaaacag cattattaac aaaagttaac
    13621 tgccataata ccatctttca cacggcaatc aagtatattg tccgaaatat ccggattaaa
    13681 ggaaaagccg tgccgctgcc tgctccctgc cgttttgcca aacctgccgc ctctttttta
    13741 agtatggctt tgctttcctg ccagctttcc cacgccgcca cggcttatat ccccccgaac
    13801 gattttcaac cgaactgcga catacgccgg ctcgggctga cacagggtca gcacaatgag
    13861 ctgcgtaaaa tccgcgccgc cttcaaaatg gcgggcgaca gggcgcgttt gaaggttatg
    13921 cattccgaac acagccgccg ccgctctgtc gtcgaaatca tttcttcgga tgtttttaat
    13981 cggaacgagg cgcgcgatta tgtcgaaagc cgctaccact ccagcatgga ttttgcggtg
    14041 gacgaattgg aaatccaaca ccgcttcttc catattctca caccgcaaca gcagcaaatg
    14101 tggctttctt cctgcctcaa ataatccccg aaacactcac ggcgcacgtt gctccggcag
    14161 cctgcccgcc cagtcgcagg caaactgcca agcggaacgt cccgagcggt tgccccgcat
    14221 ctgcgcccac tgcaacgccg ccgtccgggc ggcttcgtca tatggcacat caaaatcttc
    14281 caaccagctt cgcactgccg ccagataatc gttttgatcg aacggataaa aactgagcca
    14341 caatccgaat cggtcggaca aagatacttt ttcttccacg gcctcttttt gatggatttc
    14401 tccgcgcacg cctgtcgtac ctgcattttc atccaaatat tcgggcatca gatgccgtct
    14461 gttggaagtc gcgtacacca tcacgttggc gcaacgttgg gacagaccgc catccaatgc
    14521 cgttttcaat gccttatagg tttcgtcgcc gctttcaaat gacaaatcgt cgcaaaacac
    14581 aatgaatttt tccgggcgtt ccttcaaaag tgccaacaga tacggcaggc cgatcaaatc
    14641 gcttttatcg acttcgatca ggcgcaatcc cttatccgca tattcgtgca gcagggcttt
    14701 gaccagcgag gatttgcctg ttccgcgcgc gccgctcatc aatacgttat tcgcaggtct
    14761 gccggcaata aattgttcgg tattacgcac cagcaattcg gtctgcctgc cgactccggc
    14821 aagccgcgac aaagggaaaa cgtgcggatc gggcaagtgt tccaaaaaac cttttttgcc
    14881 cgcactctgc cagcggaagg caagcgcgtt ccaatccgta tgcccgggtt cgggcggaag
    14941 cacggcatcc aaacgccgca aaacggcata ggctttatcg aggaattcgt tcaattccat
    15001 ctctacctca ctttgcatat ctttgcgcca tcagccgttc gacggtgtcg acgattgcct
    15061 gcgtattcgg atcgatttcg atgttgatcc tgtctccggc ctttctgctg ccgaacagcg
    15121 tccgttccaa agtttcggga atcagatgga cattgaaacg gctgtcttcg actttgccta
    15181 tggtcaggct gcaaccgtcc aagccgacga accctttggt caggatatag ggtttgagtt
    15241 cgtgcgggag cgcaaaccaa accgtgcggt tgaacccgtc ccgttcgatt tcgacaatgg
    15301 gcacggttgc cataatgtgt ccgctcatga cgtgtccgcc gatttcgtcg ccgaaacgtg
    15361 ccgcccgttc gatgttgacg caatcgcctt ccttcagcag ccccaaattg gtttttgcca
    15421 aagtttctgc cattaaatcg aagctgacgc ggtttccttc gatttcagta atcgtcaggc
    15481 agcagccgtt attggcgacc gatgcgccgt gttgcaggtt ttctgccgcc tcttgcggaa
    15541 gctcgacgac ataagtctga aacgcctccg acgggcggtg gattgtcgtc agttttccca
    15601 atccttgaac aatgcctgta aacataatcc tgtttccctg tgtcggtaaa aatggtgcaa
    15661 attgtagcat ttccccgcga aaaatgccgt ctgaaactcc ttcagacggc attgtgcctc
    15721 cggttcgggc aaaaaccgcc cggtacgcct tgacctttcc tttccgcgcc ggtcggcggt
    15781 cttgcactta tccctcctgc aaatcggttt gcgtgttcaa gtcggcaaaa tgcccgtcaa
    15841 actcgaatct gaccggccgc gccctttgct gctgcaacca gcttcttaat gttttcatac
    15901 ccgaaaacag ataaggaatc gcgctttgca gaatttgcgg acggatatac ataatgttgt
    15961 agtgcatcgt taccggcgtt tccacataaa acgcattgca caacggtgtg cgtttggaga
    16021 cgctttcaaa gcgcgcgacc aaatcgtccg gcagatacgg catatcgcaa ggtacaatca
    16081 gcagccaatc ggccgccgcc aactgcaaat cgttggctgc ggtacacaat gccgaaagcg
    16141 ggccgaaatg ctgccactgc cgggcatccg gaaaaacatg cgggctgcgg cgggcgtatt
    16201 cttccaaatt ccggttggca ctgatgacga tatggctgac ctgcggcctg attttaccga
    16261 taacgcggtc aatcagcggt ctgccttcca acagtgccag ccctttgtct tccccgccca
    16321 tacggtcggc caggcctcct gccaaaatca atgcgaacgt ttttaatgcg ggcgggcttt
    16381 tgggaaagtt tgaggttttc atagtttcag tctgccgttt ccgatatttt agaagcgcgt
    16441 ttgaaaacgg cgtcgtataa attcatgtta tttgaatttt atcataacgt gatttaacac
    16501 tgaaagcatt gaggcttaag ttttccttaa cctgtccttt ctgagcagtt gtttctaatt
    16561 ccaaagaatg atattgtttg caccgtttaa aacgactttc ctccgaatat gatactgcca
    16621 acccgatttt cagacggcat ccccctttcc ctgcgcctga aactcctgac cggcttgtgg
    16681 gtcggtttgg cggcactgtc tgtcgtttta acgctgctgc tttctttccg cctggaaaat
    16741 gccgcctcgg tcatcgaaga ggcgggcaat ctgaagatgc aggcataccg gcttgcctat
    16801 atggcgggcg aaggctcgcc ccgcgcacaa atcgacaatc agattgccga atttgaaaaa
    16861 agtttaaaac gcatttccca aagcgatgcc atccatccgc tgattccctc cgacaatccc
    16921 cttgcctacg atttgataca gtccatgctg attatcgact ggcaggccaa catcctcccc
    16981 ccgttgcagg cataccgccg ccccacacaa atcgagcttt accgctttgc cggcaatatc
    17041 gaactgtttt tgcaggcgtt ggaaaatgcc ggcgaaaaaa acacttggtg gctcaggcgg
    17101 tttcaatggg tcattatgct gatgacgctg gtgtcgtccg tgctgatgct tttttggcac
    17161 cagatttggg ttatccgtcc gctgcaggcg ttaagggaag gtgcggaacg catcggtcag
    17221 cggcatttcg acatccctgt tcccgaagac ggtacgcccg aattcaaaca ggtcgggcgg
    17281 tgtttcaacc aaatggcgct caggctgaag actttatacg atgatttgga aggacaggtt
    17341 gccgaacaaa cccacaacct tgaaaagcaa aaccgcaacc tgaccctgct gtaccggacc
    17401 acgcgcgacc tgcatcaatc ctatacgccg cgacaggcgg cggaagaatt tttgaaccac
    17461 atcctgcccg ccgtcggcgc gcaatccggc aacatctgcc tggaaaacgg ttccgacaca
    17521 gacatttccg tccatacggc cgaacacggc aaaaagcccc ctttggaaaa ataccatgac
    17581 gaaaccttcc ccatcgaata tcaaaacgag aaactgggca tgctttcgct cggcttttca
    17641 gacggcactt ccctcaccgg agacgaccgc acgctgcttc aaaccctgat tcggcaattg
    17701 ggcgtatcgc tcgccggggc gaaacaggag gaagaaaaac gtctgcttgc cgtattgcag
    17761 gaacgcagcc tgattgcaca ggggctgcac gacagcatcg cgcaggcatt aaccttcctg
    17821 aacctgcagg tacagatgct ggaaaccgcc tttgccgaaa acaaacggga ggaagccgca
    17881 gaaaacatcg gctttatcaa aacaggcatg caggaatgtt atgaagatgt ccgcgaactg
    17941 ctgctcaatt tccgtaccaa aatcagcaat aaagaatttc ccgaagctgt tgccgacctg
    18001 ttcgcgcgct tcacgcaaca aaccgggata acggtcgaaa ccgtttggga aaacggttcg
    18061 ttcctgccta cacaggacga gcagctccaa atgattttca tcctgcaaga aagcctgtcc
    18121 aacatccgca aacacgcccg cgccacccat gtaaaattca ccctttccga atacggcgga
    18181 cgctttacca tgaccatcca agacaacgga caaggtttcg acacggagaa aataggagaa
    18241 cctacggaca gccatgtcgg actgcacatc atgcaggagc gtgccaaacg catccgtgcc
    18301 gttttagaaa tccgttccca agcacaacag ggaaccaccg tctcattgac gggcgcaccc
    18361 aaagaaagcc taccatgacc atcaaaatca tcctgataga cgaccacacc ctcttccgca
    18421 gcggcatcaa agcccttttg tcgcgccaac acggttttga agtcatcggc gaagctgcag
    18481 acggactctc gggcatcaaa atgatcagtc ggctgcaacc cgatgtcgtc ctgctcgatt
    18541 tggatatgcc aggcatgaac gggcgcgagg ccttgtccca aatcatcagc atcaatccgc
    18601 agcaggcagt cattatgctg accgtttccg aagacagcga cgaccttacc gaatgtatgc
    18661 gcatcggcgc gcgcggctac ctgctgaaaa acatcaacgc cgattttctt ctcgaaagca
    18721 tacgcaaagc cgccgaaggc gataatgtat tctcgcccga gatgaccgcc aaactcgtca
    18781 aaagcctgat ttccccccaa cctgcccaaa ggacgcaggc actctcctca cttacccctc
    18841 gtgaactgga aatcttgggc tatctcgccg caggacacag caacaaaatc atcgcccgcc
    18901 acctcgatct tgccgaatcc accgtcaaag tccacgttca aaacctgctc cgcaaactca
    18961 acctcagcag ccgggtgcag gccgccgttt acgccatccg gcacaacgtc ccccaacctg
    19021 tgccggaata gacgttcaga cggcatcggg tcaggaaaaa agcaatgcgc ccctttgacg
    19081 gggtgcaata tataagggac ggcatccggt gtgccgtccc tttaagtacg ccattaccgc
    19141 cgtaacccgg acggaatggc ttaggcagtt tatttccaag aatatttcac acccacggtg
    19201 ccttggtagc ctttatgtct gttgcttccg catcctgccg ataattgcct tgcctgtccc
    19261 gagaaagggt ttgattttcg ccacggcgtt ggtggagcgt atccagcatc gcatagcctg
    19321 attccaagtt ttgacgtgga acgagtgcgt aggccggtgc gcttggcgtg atgatggttt
    19381 tatcttgatt cggcgtggtt aaagtccaga agtattctgt cgcgcccgcc tcatctttgt
    19441 gtgaaaccaa ttgcaattcc cccgcccctg tggttttggc agttccgcta aaagtatcgc
    19501 gcattgtgta ggtatgttta gcgtcttcga tatttaattt accggtttcc gcccccttgt
    19561 cttgtcccaa aaccttaaca acggcggcac tgcgtttgta gcggtcggaa agttcgccga
    19621 tcgagccgtc gataatattt tctttgccat ctttacccac tgaaatgacc gttgtttttc
    19681 ctgaagcatc gcctgtgatt tccagtaaat cagattgaga atttcctcct tgatcatcac
    19741 ccggcgagtt ccattctgta ttgactttta acacaccgtc tttaccgaca taatttcctt
    19801 caatggtgag tttgtctgca taggattgat tggcaagcgt aatgatgccg ccgttggtga
    19861 atgttccttt ttcaaataca gaagttgtac ccgataaaga aatacctgta ttagataggg
    19921 ttagcgtgcc gcctgataat aatgtagtcc cttcgggggt tgttaatgat gtgtcttttt
    19981 ttaggtgaaa agtagctttt gttagtttta tggttccaat atcttctgat ttgaattcca
    20041 ttgcgccatt tgtaattttt agcacagcat tggtaatagg gagggggaag cgcattgtaa
    20101 atgatttttc tgttccatct ggattgatta atgtaccaga tgcgccaaag atctctcctt
    20161 cagggataga aacagttcca tcagaaattg atggtttatt tgcgctttta tcactgtgtt
    20221 tatgaattaa tgtaattggg gctgtactag attatcccta aattccacac cgatcccgca
    20281 ggatttttag ctgccgggac ggtgtgccgg agtta
//
LOCUS       DAXFWM010000039        20094 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000039 DAXFWM010000000
VERSION     DAXFWM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20094
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.039"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            275..1371
                     /locus_tag="ACFI8B_001292"
                     /pseudo
     CDS             275..1371
                     /locus_tag="ACFI8B_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(1418..3607)
                     /locus_tag="ACFI8B_001293"
     CDS             complement(1418..3607)
                     /locus_tag="ACFI8B_001293"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC3834510.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTGQQKASDQIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRALLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            3747..4532
                     /locus_tag="ACFI8B_001294"
     CDS             3747..4532
                     /locus_tag="ACFI8B_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC3834511.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(4631..6565)
                     /locus_tag="ACFI8B_001295"
     CDS             complement(4631..6565)
                     /locus_tag="ACFI8B_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC3834512.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6631..7809)
                     /locus_tag="ACFI8B_001296"
     CDS             complement(6631..7809)
                     /locus_tag="ACFI8B_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC3834513.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8035..8424
                     /locus_tag="ACFI8B_001297"
     CDS             8035..8424
                     /locus_tag="ACFI8B_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC3834514.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFEKYNSTKLKQNKYFKITLIKASPDHFTLQAAPDTATNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            8698..9744
                     /gene="adhP"
                     /locus_tag="ACFI8B_001298"
     CDS             8698..9744
                     /gene="adhP"
                     /locus_tag="ACFI8B_001298"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC3834515.1"
                     /translation="MKMQAVVVNKNVAGDVEVVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGTHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAVGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            9769..9963
                     /locus_tag="ACFI8B_001299"
     CDS             9769..9963
                     /locus_tag="ACFI8B_001299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834516.1"
                     /translation="MPSERCSDGIFYGFGFDFNLFCLKYRLKTLIATRHYSGLTKIRT
                     RRRAADSTNSTARRANAVPI"
     gene            complement(10045..13530)
                     /gene="smc"
                     /locus_tag="ACFI8B_001300"
     CDS             complement(10045..13530)
                     /gene="smc"
                     /locus_tag="ACFI8B_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC3834517.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            13927..14595
                     /locus_tag="ACFI8B_001301"
     CDS             13927..14595
                     /locus_tag="ACFI8B_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834518.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALGGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAVALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            14567..15052
                     /locus_tag="ACFI8B_001302"
     CDS             14567..15052
                     /locus_tag="ACFI8B_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFC3834519.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPNRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            15261..15983
                     /gene="lpxH"
                     /locus_tag="ACFI8B_001303"
     CDS             15261..15983
                     /gene="lpxH"
                     /locus_tag="ACFI8B_001303"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC3834520.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            16252..17838
                     /locus_tag="ACFI8B_001304"
     CDS             16252..17838
                     /locus_tag="ACFI8B_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC3834521.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTDLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(18004..18207)
                     /locus_tag="ACFI8B_001305"
     CDS             complement(18004..18207)
                     /locus_tag="ACFI8B_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC3834522.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            18323..18820
                     /locus_tag="ACFI8B_001306"
     CDS             18323..18820
                     /locus_tag="ACFI8B_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC3834523.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            18902..20092
                     /locus_tag="ACFI8B_001307"
     CDS             18902..20092
                     /locus_tag="ACFI8B_001307"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC3834524.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDNNIDYLCES
                     IVKV"
BASE COUNT         4329 a         5753 c         5321 g         4691 t
ORIGIN      
        1 ctgacaaacc ttgcggttcg tttccctctc cctaaccctc tcccacagga gaggggttca
       61 ggttgctgaa aatcagaggg cgaaggattg gggatcaaat ggaaggtaaa acctgaaaat
      121 gttcgggttt gacagcccga ttccctctcc ccgtgggaga gggctaggga gagggcaagc
      181 aagccgcagg cttgcctctt tggcgaaaaa tacaaccctg tccgcccaat aaatccatac
      241 ccgaaagcat cttcatacag aatcaagcca agcgatgaat aaatttttta cccaccctat
      301 gcgcccgttt ttcgtcggtg cggcagtact tgccatactc ggtgcgttgg tgttttttca
      361 tcaaccccgg cgctatcatc ctgcaccgcc aaattttctt ggaacttatg ctgccggctg
      421 catacggcgg ttttttgact accgctttgt tggaccggac gggtttttca ggcaacctga
      481 aacctgccgc tactttgatg gcggtgttgt tgcttgttgc ggctgtttta ttgccgtttt
      541 taccgcaact tgccgcattt ttcgtcgccg cctattggct ggtgttgctg ctgttctgcg
      601 cctggctgat ttggctcgac cgcaacaccg acaacttcgc cctcttaatg ctgctcgccg
      661 cattcaccgt ttttcagacg gcctatgccg tcagcggcga tttgaactta actgcgcgcg
      721 caagtgcatt tgaatatggc ggcggtcatg ttcgtatccg tccgcgtcag cgtccttttg
      781 ggcacggaaa ccctgaaaga atgccgtctg aaagaccccg tattcatccc caacgttatc
      841 tataaaaaca tcgccatcac cctgctgctg cacgccgccg ccgaactttg gctgcccgcg
      901 caaaccgccg gttttactgc gcttgccgtc ggcttcatcc tgctcgccaa gctgcgcgaa
      961 ctgcaccatc acgaactctt acgcaaacac tacgtccgca cttattacct gctccagctc
     1021 tttgccgccg caggttatct gtggacaggc gcggcgaaac tgcaaaacct gcccgcctcc
     1081 gcgcccctgc acctgattac cctcggtggc atgacgggtg gcgtgatgat ggtgtggctg
     1141 actgccggac tgtggcacag cggctttacc aaactcgact acccgaaact ctgccgcatc
     1201 gccgtctcca tccttttcgc ctccgccgtt tcgcgcgctg ttttaatgaa cgtgaatccg
     1261 atattcttca tcaccgttcc cgagattctg accgccgccg tgttcatgct ttacctgctg
     1321 acgttcgtac cgatttttcg agcgaacgcg tttacagacg atccggaata aaaatgccgt
     1381 ctgaaacggt tttcagacgg catcggattg ccaatgatca agcctcctgc ggatcgacat
     1441 ccaccgacca tcggattttg ccgtcgcggt tctgctgcaa cacctgcacc cacaaactca
     1501 cggcgcggtg caaatcttgt cgggatgttg attcgaggaa aacttgcgca cgttcgcgtt
     1561 cggcaaggcg caccatcagc atcggggcgg caccgaaccg ggagacgctt tcgggcaaga
     1621 gcggggcgag ggtttctttg gcggcgttga gaaattccat cgcatcggca acacgcggcg
     1681 catcggcacg gacggccgtc tgaaaaccga aaggcggcat ggcgaacatt tgccgttcgt
     1741 tcaattcgtt ttcggcaaac acggcgtagt cctgcgcttt gacggcggcg aagacagggt
     1801 gttcgggcag ttgggtctgt atcagcacct tgccgggttt gtcggcgcgc cccgccctgc
     1861 cggacacttg catcagctcg gcaaacagcc tttccggcgc acggaaatcc gcgctgtaca
     1921 ggctgccgtc ggcattcaac acgataacga ggttgagccg cgcgaaatca tggcctttgg
     1981 caagcatctg cgtgccgacc agaatgtcga tttcgtcgtt ggcgatgcgg cggtacaaat
     2041 ctgcccagtc gtttttgtgc gccgtactgt ccctatcgac gcggacgacg gctgccttgg
     2101 gcagcaaggc gcgcagggtt tcttcgacgc gctgcgtacc gtgtccgacg gcggtcaggt
     2161 cttggttgcc gcagtcggga catttgaacg ggatgggttc gcggtggtcg cagtggtggc
     2221 agcgcagttg gcgggcgcgt tggtgcagca ccattttggc ggagcagttc gggcagccga
     2281 aggtatggcc gcagtcgccg caaaacagcg caggcgcgaa gccgcgccgg ttaaggtaca
     2341 ccagcgacat accgcccgct tcaaagttct gtttcaaaag ctgcaaggct tgcggtgaga
     2401 agccgttatc gagtttcaga cgacctatgt tgaggatgtc cacttgtggc agttgcgcgg
     2461 aagcatgggc gcgttcggtc agttgcagca ggcggtacgc gccgctttgc gccttgtgcc
     2521 aactctccaa gctgggcgtg gcactgccca acacgacggg acagccgccc tgcttcgccc
     2581 gccacaccgc caaatcgcgg gcgtggtagc gcaattcgtt gtcttgtttg aacgagccgt
     2641 cgtgttcctc atcgaccaca atcagcccga catcaggcaa aggcgtaaac accgccagcc
     2701 gcgtgccgat caccagcttc gcctgcccca acatcgcgcg cagataatcc tgcgtgcgcc
     2761 tgcctgccgc catccggctg tgcaacacgg cggtcggcac gtcggcaaaa cggttttcca
     2821 cccgctttaa aagctgcggc gtgaggttga tttcgggcaa cagaaacaac acctgtcgcc
     2881 cctgcgccaa cactttcgcc atcgcatcga aatacacctc ggtcttgccg ctgccggtaa
     2941 tgccgtacag cagaaacgac cggaagcgtc ccaatgccgt ctgaatttga tcggaagcct
     3001 tctgctggcc ggtatttaat acaaattcag agtgcgaagc ctgcccgcgg tacggcctta
     3061 aaacaggttt cgccgcttcc gtcgtttcaa tccagccctg ctccgcccaa tcctcaatca
     3121 atttcgccgc ctgcgcgttt gcctgtttca acgccgccat cgtcatttcg cccgacagca
     3181 gcgcgtccca caaagccgct tttttgttga accgagccgg cggcggcgtt tgcgccctgc
     3241 ccgcttcgtt caaagcataa aacaacggcg gctgcggcat ttccaccgcg cgcgtttcct
     3301 tcaaaccctg cggcaatgcg gcaaatacag cctgcccagt cggatagtgg taataacgcg
     3361 acgtaaacgc caacaaatca cgccagcttt gagacagcgg cttttcttcc acaaagaccg
     3421 tctgaacgct caaaatccgc gccgcatcca tatcgggcgc aatatccgtt tcccacacca
     3481 tcccgacaac agttttattg cgaaaaggca caagcacccg cgttcccgga ggaagcggct
     3541 cggaatgcga ataagtcaaa aggccgtctg aaagcggcac gtttacagcg atgcgatggt
     3601 agatcataca ggcaggcaaa tcattttttt gcgtatttta acagccggaa gctattttca
     3661 gacgataaat tgagcctcgg cgcaatgcag gcgcaacaga ggtttgatac aatgccactt
     3721 ttccaacacg ctcaatcatc tttgatatga aaaccaagtt aatcaaaatc ttgaccccct
     3781 ttaccgtcct gccgctgctg gcttgcgggc aaacgcccgt ttccaatgcc aacgccgaat
     3841 ccgccgtcaa agccgaatcc gccggcaaat ccgttgccgc ttctttgaaa gcgcgtttgg
     3901 aaaaaaccta ttccgcccaa gatttgaaag tgttgagcgt cagcgaaaca ccggtcaaag
     3961 gcatttacga agtcgtcgtc agcggcaggc agattatcta caccgatgcc gaaggcggct
     4021 atatgttcgt cggcgaactc atcaacatcg acacgcgcaa aaacctgacc gaagaacgcg
     4081 ccgccgattt gaacaaaatc gacttcgcct ccctgccttt ggacaaagcc atcaaagaag
     4141 tacgcggcaa cggcaagctg aaagtcgccg tcttctccga ccccgattgt ccgttctgca
     4201 aacgcttgga acatgagttt gaaaaaatga ccgacgtgac ggtttacagc tttatgatgc
     4261 ccattgccgg cctgcaccca gatgccgcgc gcaaggcgca aatcttatgg tgtcagcccg
     4321 accgtgccaa agcgtggacg gattggatgc gtaaaggcaa attcccggtc ggcggcagca
     4381 tctgcgacaa tcccgtcgcg gaaaccactt ccttgggcga acagttcggc ttcaacggca
     4441 cgccgaccct cgtcttcccc aacgggcgca cccaaagcgg ttacagcccg atgccccaac
     4501 tggaggaaat catccgcaaa aaccagcagt aaacccgcaa tgcaaaaggc ttggaaccat
     4561 gttccaagcc tttttcaaat gccgtctgaa gcctgtcgcg gcagcttcag acggcatctt
     4621 tgttcaaacc tcaatcctgc gccaatgcgt ctatcggatt gagtttggct gccttattgg
     4681 cgggcataaa gccgaacgcg atgccgattc cggtcgaaca ggcgaccgcc ccgataacgg
     4741 atgccgccga aatgtccatc gggaaatcgg ttacaaaatg attgaacacg aggctgacgg
     4801 cggcggacaa acctacgccg accaagcctc cgatgatgca gattaacacc gcctcaatca
     4861 aaaactgctg caaaatattg ccgcgccgcg cgccgattgc catccgtatg ccgatttctt
     4921 tggtgcgctc ggtaacggac accagcatga tgttcatcac gccgatgccg ccgactacca
     4981 atgaaatcag ggcgatggag gaaatcagca gcttcatcgt accggtggtg ctttcgacca
     5041 tctgcctgat gctgtcgctg ttgttcataa agaagtcttc cgtgccgtgc cgtgctttga
     5101 gcagctcggc cagccctttt tcggcaaccc gggtattggc attgtctttg attttgacgg
     5161 tgatggagtt ggtgtggctc tcgcctgtga tttggtgcat caccgtcgta tagggcgacc
     5221 aaagcatcag cacgtcggaa ttgccgaaag cgttttcgtc ttttttcatc acgccgatga
     5281 cggtcaaggg gcgtttcctg aacaaaatgg ttttacccaa cggatccgag tccgcaaaga
     5341 gtttgtcttt gacattttgg tcgatgacga cgacttgcgc gtcttctttc acatcgttct
     5401 catcaaacag ccgccccgtt tccagcttca gcccgcgcac gtcgaaatat tgttcgccca
     5461 cgccgtacaa agaagcggtc aggtcggtat tgcggtaggt cagcgtgccg ccgctcgaag
     5521 tcatgggcgt ggcggaggca acgtagcttt gtttggcgat gatttttgcg tcgtctatgg
     5581 tcagggtttt gattttgccg ctgcgcctgt cgccgaagcc gcgcccgggg aagatgctga
     5641 tggtgttcgt ccccatcgaa ctgatgtctt cgaggatttt tttctgcgaa ccgttgccca
     5701 atgcgacgac ggagacaacc gaagcgatac cgatgatgat gccgagcatg gtcagaagcg
     5761 aacgcatttt gtgcgccaat actgcttgta ccgacattct gaaggcttcg acaaactggt
     5821 cgtaataaaa cgaccacgag gctttttcct gaatcctccc gacattgctt gcggggattt
     5881 cgggattttt cgaggtgtcg gaaatgattt cgccgtcccg gatttcgatg acgcggttgg
     5941 cattggcggc aatgccgggg tcgtgcgtga ccataatgac ggtatgcccg gcttcgtgca
     6001 gcctgcggat gatttccatc acatttttgc cgctggcggt atcgagtgcg ccggtcggct
     6061 cgtcggcgaa gatgatttct ccgccgttca tcagggcgcg ggcgatggag acgcgctgct
     6121 gctgtccgcc cgagagttcg ccgggcttgt tgccctcttt acttgccaaa cccaaatcct
     6181 gcaagagttt gtccgcccgc gcggaacgct ctttgcctcc catacccata tacacggctg
     6241 gcagcgcgac gttgtccctt gcggtcagcg agcttaagag gttgtagcgt tggaagatga
     6301 aaccgaagcg ttcgcgccgc aatgccgcca gttcgtcagg ctgcattttg gcagtttcga
     6361 tgccgtcgat tcggtacgaa ccggaaccgg cggtatccaa acaaccgagt atgttcatca
     6421 gcgtggactt gcccgaaccg gactgcccga tgatggcgac aaaatcaccc ttctctatcg
     6481 acaggctgat gtctttcaaa atatggacgc ggttctcgcc gctgccgaaa tagcggttga
     6541 tgtttttaca ttcgatcaag ctcataatat tccgtaaatc aataacaaat gccgtctgaa
     6601 accgtttccg tgttcagacg gcatattcgt ttatcggcgc ggcgggccgc ctagggcgcg
     6661 ttcgccgctt tcctgttgct cggcggcggt tatttcggag atgaccactt tgtccccctc
     6721 tttcaacccg cttttcactt cggtattcat actgtctctc ataccggtcc ggatttcgcg
     6781 ttccgccgcc ttgccgtccg cacccaacac gcgtacgaac gccttgccgc cgcgattttt
     6841 cacggtcagc gacggaataa gcaacacatt tttcacaccg tcgatttcaa ccgtattctg
     6901 cgtcgtcatc cccgtggcga gtttgccgtc cggattcggc acaaacgaac gggcataata
     6961 atagaccgca ttggaagccg tatccgtact gctgttgtag ccgcccgacg acatcgtggt
     7021 cagcccgggg tcgacgctgt cgagcttcgc cttaatcggc gtatccggtt cggacaaaat
     7081 cgtaaacgaa atatcctgcc ccgccttcac cttggtaata tcgccctcgg caatctgcat
     7141 tttgttcaac atcatatcca gattcgccaa ttggacaatc gtcggcgtag actgcgccgc
     7201 gttcacagtc tgcccctctt ccacgggaat cgccaccacc gtgccgtcca tcgtcgcggt
     7261 aatgcgcgtg tagcccaaat ccgactcggc ggtattgatg gaaattttgc tctgtctgat
     7321 taaagccttc aactcggcaa cattggcttt ggcggcggca agcgcatcct gcgcgctttc
     7381 caaatcttct ttagaggtcg catcatcctt ccacaacgcc gcctgacgct tatatttctt
     7441 ctccgcgctg cccaatgcaa tctgtgccga caccagcttc gcctgatacg tttccaattt
     7501 ggatttttcc atatcgagcg tgttggtctg cgtggtcgaa ttgatttccg caatcaaatc
     7561 gccctttttg acctgttgcc cgagtttgac ataaagcttt ttaatctgcc ccgaagcctg
     7621 cgcgccgacc gataccaggt tggacggcga aatctcgccc gtcgcggaaa ccgtccggct
     7681 gatgccgccg cgcctgaccg tttccgtaat ataagaagcc tgcggctcgg gcttcagata
     7741 atgccatccg ccccaaaccg ctgccgccgc gactgccgca acagccgacc atttcatcat
     7801 tttcgccata ttttcaattc tctgtatgcg tcaatcccat aaaccggaag cggattctac
     7861 cgcaagtccc tgccccatcc aaggcggcaa accgccgaca ataaaaataa ccatttgttt
     7921 taaatggctt atacaatctg ctttcccgaa aatttcaatc cgtttacata ttccgccatt
     7981 tccgatacgg cgttataatg ccgcccacaa acaaccaaat ggaaacacat tgctatgaaa
     8041 aacgttcaaa aaggctttac gctgctcgag ctgatgattg ccgtcgccat cctcggcatc
     8101 ctgacgctca tcgcctaccc ttcctataaa acctacatcc ggcgcgcccg cctgtcggag
     8161 gtcaaatcca ccctgctggt gaacgcgcaa aacttggagc gttactaccg ccaaaaaggg
     8221 acgtttgaaa aatacaactc aaccaaactg aaacaaaaca aatatttcaa gattacctta
     8281 atcaaagcca gccccgacca cttcaccctt caggccgctc ccgatacggc gaccaacgaa
     8341 ggcgaaacct gcaccgttac gctcaacgac ggcggtacca ttgccgcttc cggtacaaac
     8401 caatcctgcc ccggcttcga ctaatcctcc gccgaagccc ccaaacccgt aaaaatgccg
     8461 tccggacaca gcttcagacg gcatttttac gggtttgccc ctccgttttt tcaaacttga
     8521 aacctgccgc ccccgcatcc ggccgccgtc caatgccgcg cctccccaac tgttataata
     8581 taacatatat ttttcaacaa cataccattt gaccctcaag atgcggaagc gtatgcttac
     8641 ccttatatcg gacaacattc cgtttacagg ttttttacct tgaaaaggag cgcaatcatg
     8701 aaaatgcagg cagttgttgt gaataaaaat gtagcgggcg atgtggaagt agtcgaacgc
     8761 gaggttcgcc cgttggaata cggcgaggcg ttggtcgaag tcgaatattg cggcgtgtgc
     8821 cacaccgacc tgcacgttgc ggcaggcgac tacggcgaaa aaccgggccg cgtgctggga
     8881 cacgagggca tcggtttggt taaagaagtt gccgacggtg tgaaaaatct gaaagtcggc
     8941 gaccgcgtca gcatcgcctg gctgttccaa agctgcggct cttgcgaata ctgcaatacc
     9001 ggccgcgaaa ccctgtgccg ttccgtattg aacgcgggct acaccgccga cggcggtatg
     9061 gcgacccact gcatcgtgag tgccgattac gcggtcaaag tccctgaagg tttggatcct
     9121 gcgcaagctt ccagcattac ttgtgccggc gtgaccactt ataaagccat taaagtttcc
     9181 ggcgttcgtc ctggtcaatg gattgcgatt tacggcgcgg gcggcttggg caacttgggt
     9241 gtccaatacg cgaaaaaagt attcggcacg cacgttgtcg ccatcgacat caacgacgac
     9301 aagctggcat ttgccaaaga aaccggcgcg gatttggttg tcaacgccgc caaagaggac
     9361 gctgccaaag tgattcagga aaaaaccggc ggcgcacacg ctgcggtcgt aaccgccgta
     9421 tctgctgccg cattcaactc tgccgtgaat tgcgtccgcg cgggcggacg tgtggttgcc
     9481 gtcggactgc cgccggaatc gatggatttg tccatcccgc gtttggtttt ggacggcatc
     9541 gaagtggtcg gctctttggt cggcacgcgc aaagatttgg aagaagcctt ccaattcggc
     9601 gcggaaggtt tggttgtgcc gaaagtccaa ctgcgtgctt tggatgaagc acccgccatt
     9661 ttccaagaaa tgcgcgaagg caaaatcacc ggccgtatgg tgatcgatat gaaaaaagaa
     9721 tgcggctgcg gtcatcacca ctgatttgac gtggcggtac acatcgaaat gccgtctgaa
     9781 cgctgttcag acggcatttt ttatggattt ggatttgatt ttaatctgtt ctgtttgaaa
     9841 taccgtctga aaacccttat cgcaacacgt cattatagtg gattaacaaa aatcaggaca
     9901 aggcggcgag ccgcagacag tacaaatagt acggcaaggc gagccaacgc tgtaccgatt
     9961 taaatttaat tcactatacc gtctgaaaac ccgtatcgca acacttcatt aaaaacggca
    10021 agattcagcc gttctgcaac ccgttcaaac ggcttccgcc atttccaacg cctgcttgat
    10081 gtctacggcg acgacgcgcg acacgccttt ttcctgcatg gttacgccga ccagctgctc
    10141 cgccatttcc atggtcaggc ggttgtggga gatgtagagg aactgggttt gcaccgacat
    10201 ttctttgacc agcttgcaga aacgcgaggt gttggcatcg tccagcgggg catcgacttc
    10261 gtccagaagg cagaaggggg cggggttgag gctgaacagg gcgaacacga ggctcatggc
    10321 ggtgagggct ttttcgccgc cggagaggag gtggatggtg ctgtttttct tgccgggcgg
    10381 acgcgccatg atggacacgc cggcggtcag gaggtcgtcg cctatcattt tgagggtggc
    10441 ttcgccgccg ccgaacaggg tcgggaagaa ggtttggact ttgccgttga cggcgtcgaa
    10501 agtttctttg aaacgcgctt tggttttgtc gtcgatttgg gcgatggctt cttccaaaag
    10561 cgcgatggcg gcctgcacgt cttcgctctg gctgcggtag tagccgtcgc gttcgcgcgc
    10621 ttcttcgagt tcttgcaggg cggcgaggtt gacggcgccg agggcttcga tttgccgggt
    10681 aaggctgccg atgctgctgt tcaatacttt cgccgattct ttcgccaacg cttcgagcac
    10741 gtccagatcg gcagcgcgtt cggtcaggtt ttgatggtag cgtttggcgt tgatcagggc
    10801 ctcctgctgc tgcaacaggg cggtttgggt ggcggcctga agctgcggca gcttggtttg
    10861 cagggtttgt acgcgggcgt attgctccct gccctgttcc tgaatctgcg cgagtttttc
    10921 ctgcacggcg atgtattctt cgtccagcgt gtgtacggtt tcggtcaact cgtcgagctt
    10981 gatgtgctgc tcgtcgtttt ggaactcggt ttcataggcg agggcaagct cttgccggcg
    11041 ttcctgccag tccagggttt gctgttcgag ccgggcgatt tgctgccggt agttttgttt
    11101 ttgctggttg agtttgtgga cggcgacttc ggcaagcccg tattggcggt tggcttccaa
    11161 cagggcaagc tgcgcctgtt tcagacggcc ttgctgctct tggcggctgt gcgcggtggt
    11221 ttgctgctgg tgttcgagtt cggcggcggc ttcctgcaaa gtagcgatgt cgtctgaaag
    11281 cccgtcggac gtgtgttgca acacggtctg ctcttccgcc aactgtgcca gttcgcgctc
    11341 gatgtgttcg cggcggattt gcccttggtt ggtgcgcgcc agaagttcgg cggtgcgttg
    11401 ctgcgcttgg ctgtattggc gcgtgtgttg ctgttgctgc tgcatcagat ttttgtgctg
    11461 cacttcggac gagcgcacgg cagcctccgc ctgtttgaac gcggcttcgg cggcggaaag
    11521 tccgggggcg aggttttcca gttcggacgc gatgccgtcg aggcgcgctt tttgggcaat
    11581 caggctttcc tgcgcgggct tggcatagag caggacgctg actttatcga cctgatggcc
    11641 ttcgggtgtg agccagattt gatgcgtgcc caaatcgctt tgatgcgcga gggcgtagct
    11701 caaatcgggc gcgcacaata cgccgtcgag ccagtggtgc aatgccgtct gaaacggcgg
    11761 ctgcgcttgg atttggttca gcaatgcctg tacgggcagg gattttttga tgccgccgga
    11821 gaggccgtct gaaagccatg ccgcctgccc ctgcggcaaa ggtgcgggcg gtacgaaact
    11881 attcggcacg ctacgggcgt gcaggcgttc ggcaaggatg acggacaggg cgtgctgcca
    11941 ctcggcgggc gcggtgatgt gttgccacag ttgcggcgcg gcggcgtggt cggttgcctg
    12001 ccaaaagtcg gcggcttcct gctgttgcga caggatttgc gacaacgcct gctgctgcgc
    12061 ctgcaaggta atgtgttgct gcttcaggct ttggaagcgg tttgaggccg tctgaaacgc
    12121 ctcgcgggcg gcgtgcaagg cttcttcggc ggcaatgatt tgttcttcgt aatgctcttg
    12181 ccggctttgc aacaaggcgg cggcttcctg agcggcggcg gtttcggcct cgtcgggcag
    12241 gttcagggct tggttttcct gcttcagacg gcctttgcgc tcttcgtgct tggcaacggt
    12301 ttgttcggca tgggcaagct gctgctgctt caacgccagt tcgcggcgga tgcggtttgc
    12361 ctcgtcctgc tgggtttgga aggcagcgtt gagcgtggct tggacttctt ccaattcggg
    12421 cagacgctct tcgtgttcgg caacctgcat cgcccattcc gccaattcgg tttgcttttc
    12481 ttcggcctgc aactcgtttt cctcaagctg cacgcggatt tgctgctgct cctgccggat
    12541 gcgttgcaac tgcgcctgcg ccgcctgctt gtcgcgttcg atgcgttggt gcaggttttg
    12601 ccggtggcgg atttgttctt ccaaacgggc aatctgctcg cgcaacacgc cgcgtttgtt
    12661 gctcaattcg tgtactgcct gctgctgcga ctgttcggca gtctgcaagg cgtgtacttc
    12721 gttatttaac gcctgaacct gtgcggcggt ttcatcctgc tgcgcctgca aagattgatg
    12781 ctgcgcggtc gccttgtcgg cggcggcaag cgattgctgc cattgggcgt aatcgagcaa
    12841 atcctgttgt tgattcaact gcgcggtcag ggatttgtag cgttcggcgg tttccgcctg
    12901 tttttccagc ttttccacct gacgcgccaa ctcgttctgc aaatcgccca aacgctgcaa
    12961 atgctcgcgc gtgtctttca gacggccttc cgtctccttg cggcgttcct tatatttgga
    13021 cacgcccgcc gcctcctcga tataggcgcg caactcctcc ggccgcgctt cgatgatgcg
    13081 cgaaatcatc ccctgctcaa tgacggcata accgcgcgcg cccacgcccg tacccaagaa
    13141 caaatcggta atatcgcggc ggcgcacggt ctgattgttg atgaaataag tcgattcccc
    13201 ctgacgcgtc agctgccgct tgatgctcac ctcggcatac tgcccccacg cgccctgcaa
    13261 actgtggtcg ctgttgtcaa ataccagctc caccgaagcc ctcggcgcag gacggcgcgt
    13321 cgccgcaccg ttaaaaatca cgtcctgcat actctcgccg cgaagctgct tcgccgaagc
    13381 ctcgcccaac acccagcgca ccgcgtcaat cacattcgac ttgccgcagc cgttcggccc
    13441 gataaccgcg acaagctgcc ccggaacatg aatcgtggtc gggtcggtaa aagatttgaa
    13501 gccggagagt ttgatgtggg tcaggcgcat aatggtcgga aaaaataaaa aagaagcgta
    13561 ttttaacgga aatcccgccg caccacccca tatcttgccg gcaaaacctt accgcatccc
    13621 gccccggtgc ggcaggattt tgcacatccc ggccatccgt attaaaatct ttgcgtttcc
    13681 cacactttcc tgacaaatat acggaggagg attccatgaa aacaaaaaca gtcaagcaag
    13741 caagcaagca agcaagcaag caagcaagca agcaagcaag caaaatagtc taacccgcct
    13801 ttctttaatc gctctactta atttccactt ctttttatcc tgttcgatct tttgcggctg
    13861 cacctcaaga aataaactat tcgttatcag gcggtaaagt ttacgcagat aagttcagta
    13921 tcgccattgg ggttgaggca aaagctcagg gggataatac caaacttagc aacctcaatc
    13981 ggcacatctg ccgaagcaaa tgcacccggg gcattggctt tagggggaaa ttctgaagca
    14041 tcgaaaaaat ttagtatagc agaaggctat ctggcatcta gtgatggcta tggtgcaata
    14101 gcgattggtt ctgccgccaa gataaagcaa ttggaaaaag gcacaataaa tcatattgtg
    14161 ggaaacgata ataaaggtct ttatgtcgat gcagacggca atgtaacaaa gataaccgta
    14221 aggactgagt cggaaaaaga tattctctca agatacggtc aaacttacgg tgcggtggca
    14281 ctagggttta gatcttcttc acataatctt tttgccagtt catttggggc gttttctaca
    14341 gccacagcta ttgaaagctt ggcagtcggc gacagcagcc aatcaacggg ctaccgcagt
    14401 gctacttttg gcagtcacag cagggctttg gcagaagaaa gtttggcatt aggttatgaa
    14461 actcgggcaa atgcttatgg ttccgttgct ttaggtgcag aatctgtggc gaatgaagaa
    14521 aataccgtat cagtgggttc cgatacattg aaacggaaaa tcgtcaatgt cgccgatggc
    14581 acggaagatt tatgatgcag taaatgtccg ccagttgaac cgcttaagca aacgtacaaa
    14641 ccgcgtcggc gcaagtgctg cggcgttggc ttcgttaaaa cctgcacaat taggcaaaaa
    14701 cgacaaattc gccttttctt tgggctttgg aagctataaa aatgcccaag cggtggcaat
    14761 gggggcggtg tttaagcccg ctgaaaatgt gttgcttaat gtggcgggca gttttgccgg
    14821 accgaaccgg gctttcggcg cgggggtttc ttggaaattc ggcggcaaac cgacacctgc
    14881 ggttgccgca caaaacgcgg cgcatcctgc aaaagttttg caattgcggc aggaagtggc
    14941 ggcgttgcgc gcccggcagg cagaaaccga ccgcaaactg cacaaacagg ccgaaatgga
    15001 aaacgagctg caacagttgc gccgcgcgtt gtccgaattg aaaaaacatt gaagcaataa
    15061 acgtaaggcg ggacggcggc tctgcctttt tccgatgccg cacccgcgcg atgccgtctg
    15121 aagccttcag acggcatttt taacgcgccc gaaccccggt tccgccaatg cccgatgctt
    15181 ggcgcggcat ttgccgtatt gccccgccgc cgggcgcgtt ataatccgcc acgacatccg
    15241 cccgatttgg aaacctgcct atgcgaccct cctatttcat ttccgacctg catttgagcg
    15301 aaaagcaccc cgaactgacc gaactgctgc tgcgtttttt acgttctgcc gccgccgggc
    15361 aggcgcgggc ggtttatatt ttgggcgatt tgtttgattt ttgggtgggc gatgacgaag
    15421 tttccgagtt gaatacttcg gttgcgcggg aaatcaggaa attgtccgac aaaggcgttg
    15481 ccgtgttctt tgtcaggggc aaccgcgatt tcctaatcgg tcgggatttt tgccggcagg
    15541 cgggtatgac gctgctgccg gattactcgg ttttggactt gttcggcagc aacaccctga
    15601 tttgccacgg cgacacattg tgcaccgacg acaaggcata cctgcgtttc cgccgcatcg
    15661 tgcattgcag gcggctgcaa aaactgttcc tgatgctgcc cctgaagtgg cgcacgcgcc
    15721 ttgccgccaa aatcaggcgt gtcagcaaaa tggaaaaaca ggtcaagccc gccgatatta
    15781 tggatgtcaa tgccgccttt accgcgcggc aggttcgcgc ctttaacgcg gaaaggctga
    15841 tacacggaca cacccaccgc gagcatatcc atcacgaaaa cggctttacc cgcatcgttt
    15901 tgggcgactg gcataacgac tatgcttcaa tcctccgcgt ggacggggac ggcgcggtat
    15961 tcgtgccgcc ggaagaatgc tgaaaatgcc gtctgaagcc tttcgggcgg ctttttctac
    16021 acgccccttt cccacactcc gtccgccgca tcatcaccat ccttaacccg cattaatagc
    16081 cattatcgat aaaatgcttg accgattaca taagattacg taaagtgtgc aaacgcataa
    16141 taattggtct taccaattta tcttttaacc aaaatatcgg gtgtgcggga tttgaacgtc
    16201 tttccaaacc tcgacgcgcc ttatgatgca aatgcctaaa aaggattcgt tatggcactt
    16261 ttcctcagca tattccccat cgtcctgctg atttggctga tggtgaaaaa aaacagtatg
    16321 ccttcctacg tcgcactgcc gattaccgca gtgctgattt acgccatcaa acttttctac
    16381 ttcggcgatg cgggaatgct gctcaacgcc accgccgctt ccggcctcgt caaaacgctc
    16441 acgccgatta ccgtgatttt cggcgcgatt atgttcaacc gtatgatgga aaccacgggc
    16501 tgcatcgatg tcatccgcaa atggctggcg accatcagcc cgaaccccgt agcgcaactg
    16561 atgattatcg gctgggcttt tgcctttatg attgaaggcg catccggctt cggcacgcct
    16621 gccgcgattg ccgcgccgat tctgatgagc ttgggcttca atccgttgaa agtggcgatt
    16681 ttcactttgg tgatgaattc cgtgccggta tcgttcggcg cggtcggtac gccgacctgg
    16741 ttcggtttcg caccgctgaa cctgagtgcc gaagacatcc tcgccatcgg caggcagacc
    16801 ggcgtgatgc acttcttcgc aggtttcgtc atccctgtca tcggtttggg cttcatcgtg
    16861 ccttggtctg aaatccgcaa aaacttgggc ttcgtcgcca ttgccgtctt ctcctgcacc
    16921 attccttatg ccgcattggc gatggtcaac gaagaattcc cgtcgctcgt cgccggcgca
    16981 atcggcctga tggtgtccgt attcgccgcc aaccaaggct ggggcttgag caaagaccac
    17041 gccaaagacc cgaatgccga aaaagtgccg ttcgcccaag tcgccaaagc actcgcccct
    17101 ttgggtatgc tgatcggcat gctggtggtt acccgcatca aacagctcgg aatcaaaggc
    17161 attttgacca gcaaagaaga atggttcagc ttccaactgc cgtttgattt gtccaaaatt
    17221 accgtcagcg actccctgac gattaccttc ggcaatattt tcggacaaga tgtcagcgcg
    17281 tcttaccaaa cgctgtacgt cccggcttgg attccgttcg tgctgaccgt ttggatttgc
    17341 atcctgctgt ataaaaccaa attcaaagat gcctggacga tttatgccgt aaccttcaat
    17401 caaaccaaaa aaccgctgct tgccctgatg ggtgcgctga ttatggttca gctgatgctg
    17461 gtcggcggcg acaattcgat ggtgaaaatc atcggtaagg aatttgccgc aatggcgggc
    17521 gaacactggg tttatttttc gccgtatctg ggcgcgatcg gtgcgttctt ctccggctcc
    17581 aacaccgtgt ccaacctgac cttcggtccg attcagcagc aaatcgccct tgataccgac
    17641 ctgtccgtga ccctgattct ggcgttgcag tccgtcggcg gcgcgatggg caatatggtg
    17701 tgcctcaaca acatcatcgc cgtatgtacc gtattggacg tgaaaaattc cgaaggtgcg
    17761 attatcaaga aaaccgttat cccgatggcg atttacggcg tgattgccgt cgtcgcggca
    17821 atgattttct tcctctaggc gaaacgccgc catccatagc ggcagcacgc ggggaacgga
    17881 aaccgccgct cgtccgggcg gcgcgtcggg cagaataccc gtgccggaac agcccgcccc
    17941 gccaaacaaa tgccgtctga aaccggaaaa ggcttcagac ggcattttta tcgccgtttg
    18001 cattcaggcg tagtaatgat gcggcatctt gccgtcaagg cggtagcgcg tgccgcaata
    18061 cgggcaggca acgctgcccg attctccttc gcccaaaggt aaaaacaccc tcggatgccc
    18121 gttccactgc tcgttgtccg gtcccgagca atacagcggc agattttccg gcaacacgga
    18181 aatttcctgc ggattcagat tgtccatttg attttccttt gcgtggttgg cgtgcgccca
    18241 ttttacgcca tcggcaggct aaaggatatt ttcggcgcaa agccgcaatc cgctataatc
    18301 cccacttttc agacggcata ccatgactgc gcttaccctt cctgaagaca tccgccaaca
    18361 agaaccatcc gttctgctct ataccctcgt ttccgcctac ctcgaacaca ccgcccaaac
    18421 cggcgacgaa tccctctcct gcctgtccga cgaccagcac acgctgaccg cattctgcta
    18481 cctcgacagc caagtcgaag aaggcggctt cgtacagctt atcgcctccg gctacggcga
    18541 atacattttc cgcaacccgc ttgccgacag cctgcgccgc tggaaaatca aagccgtgcc
    18601 gaaagtcttg gacaaagcca aagccctcta cgaacaacac ggcgaaacca tcgaaacgct
    18661 cgccgacgga ggcgcagaca tcgaatccct gcgcaaacag ttccccgact ttgaagaatg
    18721 ggacggcgca tactacgaag ccgccgaaca agacctgccc ctgcttgcag aacacatcct
    18781 gtcaaaccgg gcggcattcg cccatatcgg gcaggcgtga ttgcgtctgt ttccagcccg
    18841 tgtaaaacag cgtaaaatcg gcaaacccga attatcttcc gtccccatca aggagcaagc
    18901 tatgttcttc aagcacatcg aagccgcccc cgccgatccg attctcggtt tgggcgaagc
    18961 gttcaaagcc gaaacccgcc ccgaaaaagt caacctcggc atcggcgtgt acaaagacgc
    19021 atccggcgcg acacccattg tcaaagccgt caaagaggct gaaaaacgcc tgttggaaag
    19081 cgaaaccacc aaaaattacc tgaccatcga cggcgttgcc gactacaacg agcaaaccca
    19141 aatcctgctg ttcggcaaag accacgaaat catcgccagc cgccgcgcca aaacagcgca
    19201 aagccttggc ggtacaggcg cattgcgtat tgcggccgaa tttgccaaac gccagttgaa
    19261 cgcgcaaacc atctggattt ccaatccgac ctggccaaac cacaacgcca ttgccaaagc
    19321 ggtcggtatc caagacaaac cttatcgcta ctatgatgcc gccaaacact gcttggattg
    19381 ggacggcatg attgaagatt tgaaccaagc gcaaaaaggc gacatcgtcc tgctgcacgg
    19441 ctgctgccac aaccctaccg gtatcgaccc tacgcccgaa caatgggaaa ctttagcaaa
    19501 actttctgcc gaaaaaggct ggctgccgct gtttgacttt gcctaccaag gctttggcaa
    19561 cggtttggaa gaagatgcct atggcttgcg cgtgttcttg aaacacaata cagaattgct
    19621 gattgccagc tcttattcca aaaacttcgg catgtacaac gagcgcgtcg gcgcgttcac
    19681 tttggtggcc gaagatgaag aaacagcagc ccgcgcccac agccaagtca aaaccatcat
    19741 ccgtaccttg tattccaacc cggcttcaca tggtgcgaac accattgcac tggtgttgaa
    19801 aaatgatgat ttgaaagcac aatggattgc cgaactcgat gaaatgcgcg gccgcatcaa
    19861 agccatgcgc caaaaatttg tcgagttgct caaggccaaa ggttcaaccc aagactttga
    19921 ttttattatt gaacaaaacg gtatgttctc tttcagcggc ttgactcccg aacaagttga
    19981 ccgtttgaaa aacgagtttg ccatttatgc cgtccgttcc ggccgcatca acgtcgccgg
    20041 cattaccgac aacaacatcg attatctgtg tgaaagcatc gtgaaagtat agtc
//
LOCUS       DAXFWM010000040        20000 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000040 DAXFWM010000000
VERSION     DAXFWM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20000
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.040"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..331)
                     /locus_tag="ACFI8B_001308"
                     /pseudo
     CDS             complement(1..331)
                     /locus_tag="ACFI8B_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            335..643
                     /locus_tag="ACFI8B_001309"
                     /pseudo
     CDS             335..643
                     /locus_tag="ACFI8B_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(718..2772)
                     /gene="metG"
                     /locus_tag="ACFI8B_001310"
     CDS             complement(718..2772)
                     /gene="metG"
                     /locus_tag="ACFI8B_001310"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC3834525.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNGKIEDTDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(2888..4726)
                     /gene="glmS"
                     /locus_tag="ACFI8B_001311"
     CDS             complement(2888..4726)
                     /gene="glmS"
                     /locus_tag="ACFI8B_001311"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC3834526.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRIGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            4998..5501
                     /locus_tag="ACFI8B_001312"
                     /pseudo
     CDS             4998..5501
                     /locus_tag="ACFI8B_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5805..7130)
                     /locus_tag="ACFI8B_001313"
     CDS             complement(5805..7130)
                     /locus_tag="ACFI8B_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC3834527.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPIHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKSYMRQNPHKLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7363..8183
                     /gene="efeU"
                     /locus_tag="ACFI8B_001314"
                     /pseudo
     CDS             7363..8183
                     /gene="efeU"
                     /locus_tag="ACFI8B_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            8180..9346
                     /gene="efeO"
                     /locus_tag="ACFI8B_001315"
     CDS             8180..9346
                     /gene="efeO"
                     /locus_tag="ACFI8B_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC3834528.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDACEDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            9654..10919
                     /gene="efeB"
                     /locus_tag="ACFI8B_001316"
     CDS             9654..10919
                     /gene="efeB"
                     /locus_tag="ACFI8B_001316"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC3834529.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWGRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGDITPKDSHMRLANPR
                     DPEFLKKHCLFRRAYSYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(10991..11320)
                     /locus_tag="ACFI8B_001317"
     CDS             complement(10991..11320)
                     /locus_tag="ACFI8B_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC3834530.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(11395..12765)
                     /gene="glmU"
                     /locus_tag="ACFI8B_001318"
     CDS             complement(11395..12765)
                     /gene="glmU"
                     /locus_tag="ACFI8B_001318"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC3834531.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPAGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(12820..13092)
                     /locus_tag="ACFI8B_001319"
     CDS             complement(12820..13092)
                     /locus_tag="ACFI8B_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC3834532.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(13085..13798)
                     /locus_tag="ACFI8B_001320"
     CDS             complement(13085..13798)
                     /locus_tag="ACFI8B_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC3834533.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTPPENTKECYA"
     gene            13857..14858
                     /locus_tag="ACFI8B_001321"
     CDS             13857..14858
                     /locus_tag="ACFI8B_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3834534.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSLEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            14952..15800
                     /locus_tag="ACFI8B_001322"
     CDS             14952..15800
                     /locus_tag="ACFI8B_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC3834535.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            15826..16311
                     /locus_tag="ACFI8B_001323"
     CDS             15826..16311
                     /locus_tag="ACFI8B_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC3834536.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDA
                     V"
     gene            complement(16395..17963)
                     /gene="msrAB"
                     /locus_tag="ACFI8B_001324"
     CDS             complement(16395..17963)
                     /gene="msrAB"
                     /locus_tag="ACFI8B_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC3834537.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGTATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            18109..19362
                     /gene="ftsY"
                     /locus_tag="ACFI8B_001325"
     CDS             18109..19362
                     /gene="ftsY"
                     /locus_tag="ACFI8B_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC3834538.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRGRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
     gene            complement(19398..19646)
                     /locus_tag="ACFI8B_001326"
     CDS             complement(19398..19646)
                     /locus_tag="ACFI8B_001326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834539.1"
                     /translation="MPSEPSDGIFDAPAVYRRGAGAVKYPNRHSRQHRNLETRPTTPQ
                     SRNPSFPRRRESRSVRTETYRIKTVAQTPRPRFPLPWE"
     gene            complement(19660..>20000)
                     /locus_tag="ACFI8B_001327"
     CDS             complement(19660..>20000)
                     /locus_tag="ACFI8B_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3834540.1"
                     /translation="TGSRFKPYAGVRVAYGHVKHQVRSVEKETTFVLSKTLGVEKPGI
                     IKDAPTRKLPHHESRSISSLGFGAVAGVGIDITPKLTLDAGYRYHNWGRLENTRFKTH
                     EASLGVRYRF"
BASE COUNT         4092 a         5126 c         6146 g         4636 t
ORIGIN      
        1 catgtttatc acggcaggtt gacggcaggt gcttggtttc gattttggtg ccggtggtgt
       61 cggcggtgac ggcgtcggtg ccggctttgg cggcgtcgtc gcgctgaacc ggctgtccgc
      121 agaaccattt taccgaaccg ttttcacgct tggcccacag ggagagtttt ttgtctttga
      181 tttcattgtt tacgccgctt ggattcattt gggcggtaac gacgccgttt gtgaccgtaa
      241 cgcttttaac atatttgcct ttgatttctg aagcggatgc cacgccggca tcgccgttgt
      301 ttgcgggcca ttcgccgtga ttcaggtaat acccggcgaa aacgagttgc ggcagatgaa
      361 gccgggtgcg gttttgatca attgcgggcg cggcgggctg gcggatgaaa acgcgctgct
      421 tgccgcactc aaatacgggc agatcggcgg ggcgggtgtc gatgttttga cggaggaacc
      481 gccgaggggc ggcaatccct tgctgaatgc acgattaccc aatctgattg ttacgccgca
      541 taccgcgtgg gcgagccgcg aggctttgga tcggttgttt gaaatattgt tggcgaacat
      601 tcacgccttt gtgaaaggcg aggcgcaaaa ccgcgtggtt tgaacctgtc gggattgcgg
      661 aaaaaaacgc cgtctgaacg cccaaggggt tcagacggcg ttttttgaga tttctgccta
      721 accgactttg tcgcccggct gagcgcccgc atccacgtca agcagcttca gtttgccgtc
      781 tgccgtggcg gcggagagga tcataccttc ggatacgccg aattttgcca ttttgcgtgg
      841 ggcgaagttg gcgactgcga tgaccatgcg accgttcaat tcggcaggat ttgggtaaga
      901 cgcggcgatg ccggagaaga tgatgcgttt ttcaaaaccg aaatcgaggt cgaatttcaa
      961 aagtttggtg ctgccttcga cggcttcgca gttcaatact ttggcgacgc gcatatcgat
     1021 tttcataaag tcgtcgaagc ttgcctgttc ggcgactttt tcgtatttac cctcttcaac
     1081 gggcgcggat gccgtctgaa tgctttgttt gttggcttcg atcaaatcgt ccacttgttt
     1141 ttgctccact cgttgcatta aatgttcgta tttgttgatg gcgtgttcgc ctagggtttc
     1201 gcgcgtgttc ttccaggtaa tcgcgtccag attgaggaaa cgcgcggcgt tggcggcggt
     1261 ttgcggcaac acgggggcga ggtaggcggt cagcatggtg aaggcgttga tgagttcgct
     1321 gcatacttcg tgcaggcgtt cgtcttgacc ttcttgtttg gcgagctccc acggcttgtt
     1381 ggcatcgacg tattcgttga caatgtctgc caatgccatg atgtcgcgca gggcgcgggc
     1441 gtattcgcgg ttttcgtatt gctcggcgat ggtgtcgctt tcggcggcga gttttgccag
     1501 caatgcgctg cctgaaacgt ctttcggacg accttcaaag cgtttggcga tgaaacccga
     1561 agcgcgggcg gcgatgttga cgtatttgcc gacaaggtcg ctgttgacgc gggaaataaa
     1621 gtcttgcagg ttcaaatcgg tgtcttcgat tttgccgttg agcttggcgg cgatgtagta
     1681 gcgcatccac tcggggttca ggccttgttc cagataagat ttggcggtga tgaaggtgcc
     1741 gcgcgatttg gacatttttt gtccgtccac ggtcaaaaag ccgtgtgcgt acacgccggt
     1801 cggggcgcgg tggccggaga aatgcagcat ggcgggccag aacagggcgt ggaaatagag
     1861 gatgtctttg ccgatgaagt ggtacatctc ggtttggctg ccggctttga agtattcgtc
     1921 gaaatcgacg ccgatgcggt cgcacaggtt tttaaacgac gccatatagc cgacgggcgc
     1981 gtccagccaa acgtagaagt atttgcccgg cgcgtcgggg atttcaaaac cgaaatacgg
     2041 cgcgtcgcgg gaaatgtccc agtcggacag ggtggtttct ccgccttcgc ccagccattc
     2101 cttcattttg ttgagggctt cgggttgcag atgaggcttg ccgtcgtgcg ggttgttgcc
     2161 ggaagtccat gctttgagga agtcggcgca ttcgcccagt ttgaagaaga agtgttcgga
     2221 ttcgcgcaat tcgggtttcg cgccggaaac ggcggaatac gggttgaaca gttcggtcgg
     2281 ggaataggtc gtgccgcaga cttcgcagtt gtcgccgtat tggtcttggg cgtggcattt
     2341 cgggcattcg cctttgacga agcggtcggg caggaacatt tgtttttcgg ggtcgaaaag
     2401 ctgctcgatg acgcggcttt cgattttgcc gttggctttc agcgcgcggt aaatgtcttg
     2461 ggaaaactgt ttgttttcag tggaatgggt gctgtaataa ttgtcgtagc cgatgaaaaa
     2521 gccggtaaag tcggcaaggt gctctttgcg cactttggca atcatgtctt cgggcgcgat
     2581 gccttgtttt tgcgcggcaa gcataacggg cgtgccgtgg gtgtcgtcgg cgcagcagta
     2641 gtagcattcg tggccgcgca gtttttgaaa gcgcacccaa acgtcggttt ggatgtgttc
     2701 gaccatgtgg ccgaggtgga tgctgccgtt ggcatagggc agggcggagg taactaagat
     2761 tttgcgtgtc atattgtgct ttgcaaacaa tgggtaaagg cggattatac cgcaaatcaa
     2821 acggggaaat gccgtctgaa gcctgaaaaa tcgggcttca gacggcattt ttgccaaccg
     2881 gcgggattta ttcgacggtt acggatttcg ccaggttgcg cggcttgtcc acatccgtgc
     2941 cgcgcgcgag ggcggtgtgg taggcgagga gctgcacggg gacggtatgc acgacggggg
     3001 acagttcgcc gacgtggcga ggtgcgcgga taacgtgcac gccttcggtg gcattaaaat
     3061 tgctgtcgag gtcggcaaag acgaaaagtt cgccgccgcg cgcgccgact tcctgcatat
     3121 tggctttgac tttgtccaac aggctgtcgt tgggcgcgat gacgacgacg ggcatgtttt
     3181 cgtccaccaa ggcaagcggc ccgtgtttca gttcgccggc aggataggct tcggcgtgga
     3241 tgtaggtgat ttcctttagc ttcaacgcac cttcaagggc aatcgggtaa tggatgccgc
     3301 gccccaaaaa cagcgcgctg gttttcttgg caaactgttg cgcccaagcg gcaatttgcg
     3361 gttcgaggtt caaagcgtgc tgcacgctgc cgggaagctg gcggagttct tcggtgtaac
     3421 gtgcttcatc ttcgccggat accacgccgc gcactttcgc cagcgttacc gccaaaccga
     3481 acagcacgac cagttgcgtg gtaaatgctt tagtcgaggc gacgccgatt tccgcaccgg
     3541 cacgggtata aagcacaagg ctgctttcgc gcggcagggc ggattccatc acgttgcaga
     3601 cggagaggct gtggcggtgt cccaaagatt tggcgtattt caaagcctcc atcgtgtcca
     3661 gcgtttcgcc ggattgggaa atggtaatga ccagttggtc ggggtcggca atcacgctgc
     3721 ggtaccggta ttcgcttgcg atttcgacgt cggccgggat tttggcgatg gattccaacc
     3781 aatatttggc ggtcagcgcg gcgtaatagg acgtgccgca ggcaaggatt ttgacgctgc
     3841 ggatgctttc aaacacgctt ttggcatttt tgccgaagtt ttcggggatg aatccgccgt
     3901 cgaggaaaac ttccgccgtg tccgcaatcg cgcgcggctg ctcgtggatt tctttttgca
     3961 taaagtggct gtgcggaccc ggttccaaag aggcgggcga gagttcggat accttgactt
     4021 tgcgttcggc aggcaggccg tttttatcgg tcagccgttt gatgccgtct gaagccagca
     4081 gcgcgatgtc gccgtcttcg aggtacgcca cgcggcgcgt aaaggcgatg acggcggata
     4141 cgtccgaagc gataaaggtt tcatcgtcgc ccaaagcgac caaaagcggg cagcccatac
     4201 gcgccacaac taattcatca ggcttgtctt gggcaataac cgcgatggcg tatgcgccgt
     4261 ggaaacgttt gaccgcttcc tgcacggctt caaacagcct gccgccgttt tgcgcgtatt
     4321 cgtggttgat gctgtgtgcg atgacttcgg tatccgtttg cgattcaaac cggtatccca
     4381 aaccttccaa gcgtttgcgt tcgctttcaa agttttcgat gatgccgttg tgtacgaccg
     4441 caatcatacc gccgctgatg tgcgggtggg cattcggctc ggtaacgccg ccgtgtgtcg
     4501 cccagcgcgt atgcccgatg ccgatgccgc cgctgatgcc tttttcgcgt gccgcgtcct
     4561 ccataagctg cacgcgtccg atgcggcgca cgcgtttgat tttgccgtcg gtgttgacgg
     4621 caatgcctga tgagtcgtaa ccccggtatt cgaggcgttt gaggccgtcg gtcagaaaat
     4681 cgacgacgtt gtgatgggcg cggatggcgc cgacgatacc gcacataaac tgttccttag
     4741 tatccggttg aaaaaaaaga tgcggacggc ttccgtaccg caccttccgt ttcggattat
     4801 aaaccgcctc ccgcgccgga aaacagcaaa atgccgtctg aaggcttggg cttgctcaaa
     4861 aaagggaggg atttccccgc ctgaccggga tgggcgttca gacggcattg cctgccgccg
     4921 gtttatagtt tttgcaaatc aatattaaca agctgaaaaa aaacaatata cccgcccggt
     4981 cttaatgtta acggagtatg gaaatgaaac aaatgctttt ggccgtcggc gtggcggcgg
     5041 tgttggcggg ctgcggcaag gatgccggcg gttacgaggg ttattggcgc gaaaagtcgg
     5101 acaaaaaaga gggcgtgatt gcggtcaaaa aaaaagtcaa ttacttcctt aataaaatca
     5161 acgtgtttac aggcaaggag gagtctttgc ttttgtctga aaaagacggc gcgctttcga
     5221 taaacacggg gataggggaa atcccgatca aactttccga cgacgggaaa gagctgtatg
     5281 tcgaacgcag gcggtatgtg aaaaccgatg cggcgatgaa ggacaaaatc atcgcccacc
     5341 agaaaaagtg cggacaaacg gcacaggcat acctcgacgc gcgaaatgcg ttgccgtcaa
     5401 accaaacgta tcagcagcgt caggcggcga tcgagcaatt gaaacggcgg tttgaagccg
     5461 agtttgacga attggaaaaa gaaatcaaat gcaacggcag aaccccggca ttgttgcttt
     5521 ggcaggggac aaccgggagg atgccgccgt ccgaatcgga cgcgtggttt ccataccggt
     5581 acgggcgggc gggaatgtcc gccttttttt gttcggatgc gtttgaatac ccgtccgatt
     5641 ctgtccgttt gcaaggggga tttccgttcg ggcggaaatc cattccgtca aataagaaag
     5701 gcgttttgtc cggccggccg gcttgtgcct gcggtatcgc aaaacttcgc cttaatgcgc
     5761 ccgattggct agggacgggc ttcggacggc attgttttgc cggttcacgg gcggtattcg
     5821 ggcttcatgc cgttgggcag gagctgccag acgtatcccg tggttttctg tttgccggca
     5881 agttcgccgg cttcgtcgcc gtaaccccaa aaataatcca cgcgcaccgc gcctttgatc
     5941 gcgctgcctg tatcctgcgc cataatcagg cggttgaggg ctttgcgggt aaccggatgg
     6001 gcggtggcga caaataaggg cgcgcccaag gtaatgtagt gccggtcgat tgcgccggcg
     6061 tattccccca tcagcggcgt gcccagtgcg ccgacggggc cctcattgcc gcttccggca
     6121 agctcgcgga aaaagatata gctggggttt tgacccaaaa cttcggcgag tttgtgcggg
     6181 ttttgccgca tatatgactt aatgccctgc atggaggttt gcccgagctt gaggtagcct
     6241 ttgtccgcca tatagcgtcc gatggaaaca tacggatgct cgtttttgtc ggcatagccg
     6301 atgcggatgt atttgccgga cggggttttc agacggcccg agccttgtat gtgcatgaaa
     6361 aaaagttcga cggggtcttc ggcgtaaccg agtatcgggg ctttgccgtc aagcgcgccg
     6421 ccgttgattt ggttgcgcgt gtggtagggg aggaagcggc ttccttcaaa cctgcccttg
     6481 attgccgttg tgcgcgcggt aatggggaat tgggaaaggt cggcggtatg cgtgccgccg
     6541 gcattgtcga ttgtgccgct gttttttccc gtctgcctga tgcggacaag ggctttcccg
     6601 ccccgcaaac cggcaggcag ggggacggag ataaaatcgt cgggaatgcc gtaaatcggg
     6661 aagcgggccc gttccgtccg cctgccgtcg cccttcagca ccggttcgta atagccggta
     6721 accgtacctg caaggcttcc gttgcctgca acctgccaag gcgtgaaata gcgttcaaaa
     6781 aaccgctttg cctgaaagga atggatgggg gtttgaaagg cttgggcgca cacatcctgc
     6841 cagccttggc ggtttttcaa attggcgcag ccgaggcgga aggattgcag gcttttggca
     6901 aaatcctgcg ccgcccagtg gggcatggac aggtgcggca caacggtata gacggccccg
     6961 ccgccggcaa ccgtcgttcc ggcggggtcg gggatgccgg ccggccggtc cgggccgttg
     7021 atgacggatg tgtcgggttg cggaaaggtt tggatgctcc tgctttggca ggcggcgagg
     7081 atggcggcgg cgatgccgta cagggcggag cggagcaggt gttttttcat aatggacaat
     7141 gttgtcgtca gtaataaaaa aagatggttt cgggcggcgt tgcggtagcc ggggagaggg
     7201 gattttaaca cagggcgcag ccgcagcctg cggaactttc cgccacgcgg caatgcagat
     7261 aaaaataact tgcatttgta tttgcaagta atgaaaatat tccgataata tattattaat
     7321 catccttgtt cattcgcgtt tacgctggtc gcttttttaa ttatgttgcg cgagggtatc
     7381 gaagccgcgc tcatcgtcgg catcgttgcc ggttttttga aacagtccgg acattcaaaa
     7441 actgatgcct aaggtctggt tcggggtcgt ccttgcttct ttgatgtgtt tgggcatcgg
     7501 atacggcatc cattcggcaa cgggcgagat tccccggaag cagcaggagt tcgtcgtcgg
     7561 cattatcggt ttggttgccg tcgcgatgct gacttgtgtg gttttatgga tgaaaaaggc
     7621 ggcgcgttcg atgaagcgcc agcttcggga ttctgtacag gcggctttga accgtggcag
     7681 cggtcaagga tgggccttgg tcggtatggc gtttcttgcc gtggcgcgcg aaggtttgga
     7741 gagcgttttt tttcctgctt gccgtattca aacagagccc gacgtggcag atgccggccg
     7801 gcgcggtagc gggggttttg gctgccgccg tgattggcgc gttgatttat cagggcggga
     7861 tgcgcctgaa tctggcgaag tttttccgtt ggacgggggc gtttctgatt atcgttgccg
     7921 ccggtctgct tgccggctcg ctgcgtgcgc tgcatgaggc aggcatttgg aacgcgcttc
     7981 aggacattgt gttcgaccca tcaaaatatt tgcacgaaga cagtccgttg ggtgtgctgc
     8041 tcggcggatt tttcggctat accgaccatc cggcgcaggg cggggccttg gtttggctgc
     8101 tgtaccttat tcccgtcatg atttggtttt tgcgcggcag caggccgtct gaaactttaa
     8161 cccgtaaaga ggaattgaaa tgagaaaatt caatttgacc gcattgtccg tgatgcttgc
     8221 cttgggtttg accgcgtgcc agccgccgga ggcggagaaa gccgcgccgg ccgcgtccgg
     8281 tgagacccaa tccgccaacg aaggcggttc ggtcggtatc gccgtcaacg acaatgcctg
     8341 cgaaccgatg aatctgaccg tgccgagcgg acaggttgtg ttcaatatta aaaacaacag
     8401 cggccgcaag ctcgaatggg aaatcctgaa gggcgtgatg gtggtggacg aacgcgaaaa
     8461 tatcgccccg gggctttccg acaaaatgac cgtaaccctg ctgccgggcg aatacgaaat
     8521 gacctgcggc cttttgacca atccgcgcgg caagctggtg gtagccgaca gcggctttaa
     8581 agacaccgcc aacgaagcgg atttggaaaa actgccccaa ccgctcgccg actataaagc
     8641 ctacgttcaa ggcgaggtta aagagctggc ggcgaaaacc aaaaccttta ccgaagccgt
     8701 caaagcaggc gacattgaaa aggcgaaatc cctgtttgcc gacacccgcg tccattacga
     8761 acgcatcgaa ccgattgccg agcttttcag cgaactcgac cccgtcatcg atgcgtgtga
     8821 agacgacttc aaagacggtg cgaaagatgc cgggtttacc ggcttccacc gtatcgaaca
     8881 cgccctttgg gtggaaaaag acgtatccgg cgtgaaggaa accgcggcca aactgatgac
     8941 cgatgtcgaa gccctgcaaa aagaaatcga cgcattggcg ttccctccgg gcaaagtggt
     9001 cggcggcgcg tccgaactga ttgaagaagc ggcgggcagt aaaatcagcg gcgaagaaga
     9061 ccgttacagc cacaccgatt tgagcgactt ccaagctaat gcggacggat ctaaaaaaat
     9121 cgtcgatttg ttccgtccgt tgattgaggc caaaaacaaa gccttgttgg aaaaaaccga
     9181 taccaacttc aaacaggtca acgaaattct ggcgaaatac cgcaccaaag acggttttga
     9241 aacctacgac aagctgagcg aagccgaccg caaagcatta caggctccta ttaacgcgct
     9301 tgccgaagac cttgcccaac ttcgcggcat actcggcttg aaataagccg caagcgttca
     9361 gacggcattt ggcggcagat accgtctgaa gttttatagc ggattaacaa aaaccggtac
     9421 ggcgttgccc cgccccggct caaagggaac ggttccctaa ggcgcccaag caccgggcga
     9481 accggttccg taccatttgt actgcctgcg gcccgccgcc ttgtcctgat ttttgttaat
     9541 ccgctatatc cgccatatgt tgcagggcgg gctttcagac cgccgctatc ggttaatgga
     9601 aaaacggcgt gcagggatgc ccaccctgct gcacggatat tgaaggaaac gccatgagtc
     9661 aaaaccaacc cgcacaaccg accaaacgca atctgttcaa aaccgccctt gccgtcggcg
     9721 caatcggcgc aatcggaggt tatttcggcg gcaaaaaaca gggcgaaacc gccgaacgca
     9781 ccgccgaaag ccaacactcg ccccaagcct atccctgcta cggcgaacat caggcaggta
     9841 tcgttacgcc gcggcaggcg ttttccatta tgtgcgcctt cgacgtaacc gcgcaaagtg
     9901 ccaagcagct ggaaaacctg ttccgcacac tgaccgcccg catcgagttt ctcacccaag
     9961 gcggagaata ccaagacggc gacgacaaac tcccgtcagc cggcagcggc attttgggca
    10021 aagccttcaa ccccgacgga ttgaccgtta ccgtgggggt gggcagcagc ctgtttgacg
    10081 gccggttcgg actcaaagac aaaaaaacgg ttcatttgca ggaaatgcgc gacttcccca
    10141 acgataagct gcaaaaaagc tggtgcgacg gcgatttgag cctgcaaatc tgcgccttca
    10201 cccccgaaac ctgccaagcc gccctgcgcg acatcatcaa acacaccgcc caaaccgccg
    10261 tcatccgctg gagtatcgac gggtggcagc ctaaatccga acccggcgcg atggcggcgc
    10321 gcaacctgtt gggcttccga gacggcacgg gcaaccccaa ggtttccgat cccaaaaccg
    10381 ccgacgaggt tttatggacg ggcgtggccg ccaacagcct cgacgaaccg gagtgggcga
    10441 aaaacggcag ctatcaggca gtccgcctta tccgccgctt tgtcgagttt tggggcagga
    10501 cgccgcttca agagcaaacc gacattttcg ggcggcgaaa atacagcggg gcgccgatgg
    10561 acggcaaaaa agaagccgac caaccggatt tcgccaaaga ccccgagggt gatatcacgc
    10621 ccaaagacag ccatatgcgc ctggcgaatc cgcgcgatcc cgaattcctc aaaaaacact
    10681 gcctcttccg ccgcgcctac agctattctc gcggacccgc ctcaagcgga cagcttgatg
    10741 tcgggctggt gttcgtctgc tatcaggcaa atcttgccga cggtttcatc ttcgtgcaaa
    10801 acctcctcaa cggcgaaccg ctggaagaat acatcagccc cttcggcggc ggctatttct
    10861 tcgtcttgcc cggcgtggga aaaggcggat tcttgggaca agggctgccg ggcgtataaa
    10921 cctgcctgac ggacaaatgc cgtccgaacc ttgccaaacg ggttcggacg gcattttact
    10981 gtttggacgg tcaggctttt ttgacgaatt cggattttaa attcatcgcg ctgccgtcga
    11041 ttttgcagcc gatgttgtga tcgccttcct gcaggcgtat gcctttgacc ttcgtacctt
    11101 gtttgatcgt catcgagctg ccttttacct tgaggtcttt gatgaggatg acggtgtcgc
    11161 cgttttgcag cgccgcgccg ttggcatcgc gcacttgagc cgcaaggtcg gcggcggatt
    11221 cggtttcatt ccattcgtgg gcgcattcgg ggcagatgta ttgtccgccg tcttcatagg
    11281 tgtattcgga ggcgcattgc gggcaagggg gtaatgacat ggtttgccgt ccttatcgga
    11341 tgtttgtttt gggatgccgt ctgaaacctg aaaccggctt cagacggcac ggctttattg
    11401 tttgtctttt tccggcctca tccagccttc gatgacggtt tggcgggcgc gggcgagggc
    11461 gagtttgttg tcttcgatat tgcgggtaat cgtgctgcct gcgcctgtgg ttactttgtt
    11521 gcccagtgtt acaggggcaa ccagtacgca gttcgaaccg atgcgcactt cgtcgccgat
    11581 gacggttttg tgtttgtgca cgccgtcgta gttggcaata atcgtgcccg cgccgaagtt
    11641 ggttttgctg ccgacttcgg cgtcgcctat gtaggtgagg tggttggctt tggtgccttt
    11701 gccgatggcg gcgtttttga tttcgacgaa gttgccgacg tgtacattgt cagccagttt
    11761 ggcttgcgga cgcaggcggg cgtacgggcc gattcggttg ttttcgccga cttcgcagcc
    11821 ttcgaggtgg gagaaggggg caattttgct gtttgcgccg attttggcgt ttttgatgac
    11881 gcagtttgcg ccgatttcga cgttgtcgcc gagttcgact tcaccttcga tgacgacgtt
    11941 gacgtcaatc acgacatcct gtccgtgttt cagacggccc cgcaaatcga aacgcgccgg
    12001 atcgcgcagg gtcacgcctg ctttgagcaa ttcttgcgcc tgttcggttt ggaagatgcg
    12061 ttcgagttcg gcgagctggc gtttgttgtt cacgccggcg gcgaggtggg aggcgcgcac
    12121 ccggacgggg cggactttga tgccgtcggc aacggctttg gcgatgaggt cggttaaata
    12181 atattcgcct tgggcattgt tgctggaaag gctgttcagc cagttttcca gtttggcgtt
    12241 gggcaggacg aggatgcctg tgttgatttc gcggatggtt ttttgggtgg cacttgcgtc
    12301 tttttcttcg acaatggcgg ttacgctgcc gctgccgtcg cggatgatgc gccccaagcc
    12361 tgccgggtcg gcgggcacgt cggtcaacag cccgacttcg ttgcctgcgg cttcgagcag
    12421 ggtttcgagg gtttcaacgt caattaaagg aacgtcgccg tacaacacca gcgtgcggcc
    12481 ttcggaggca aggtggggca gggcggtttt gacggcgtgg ccggtgccga gttgttcggt
    12541 ttgttcgacc cagacggcat cgcgtttgac agtgtctaaa acttgctctt tgccgtgtcc
    12601 gacgacgacg cagatgtttt gcggattcag cgcggcggcg gtgtcgataa cgcgctcgac
    12661 catgggcttg ccgccgatgc agtgcagcac tttgggcatt tgggaataca tgcgcgtgcc
    12721 tttgccggcg gcgaggatga cggtgtttaa agtgttttgc ggcatagcgt tttcctgtgc
    12781 catgccgtct gaagcggttt cagacggcat agggtaggtt tatcggtttt gaaactttgg
    12841 tttttgccag tgttggcgat gctcttcgtc ggcgttgttg ccggtttggt cgggcagcac
    12901 ggcgcggcgt tcgggacggt attggttgta gttcatattt ttcgagtagc tgccgtcttg
    12961 gtaataaacg ggcgtgccgg cgggatattt ttgacggacg gcggttttgc cgttgccgtc
    13021 ttgataagtt tcccacgcgc agcccgacaa aagggcggcg gagacggtca ggataaggaa
    13081 ggttttacgc atagcattct ttcgtatttt cggggggggt gcgggtattg taatgatttt
    13141 ggcggcgttc tgacaaagtt tctgcatacc gtgccagttg cgccatatcg cttacggagg
    13201 catcgataaa gggcggcgtg tgggattttg caccgaaccg gacggttttc atgcccaatg
    13261 cttttgcctg atggaggttg tccgcgctgt cgtccaccat aatgcagcat tcgggcggta
    13321 cgtccaacag gcggcagaca ttgagatacg cttgcggatt gggtttgtac agcagtccga
    13381 aatcatccgt gccgaacagt gcgtcgaaac agttttccaa accgagtgcg ccggcaacgg
    13441 cacggacgta aaacgacggg ccgttggaaa aaaccgcctt gcgcccgttc agacggcata
    13501 gggtgttttc cgtatccgcc atgccgtgca gcctggtcag gattgcatcg atcggatggc
    13561 tttcgcgtaa aaattccgca atgtcgattt cgggatggtg gatttgcagt ccggcgagcg
    13621 ttgcgccgta acggtgccaa tagtcttgac gcaggtcgga cgcggcggat tcggagagtt
    13681 tgaggcggcg tgccatatag cgcgtcatag cgcggttgat gaggtggaag atgcctgcgt
    13741 cggcatcgtg cagcgtgttg tcgaggtcga acagccacac ggtcgggttt tcttgcattt
    13801 tgaaccgtga aaatttgtta gaatgttatt ttacagcgaa taaaggaaga ctcggaatga
    13861 aacggaaaat ttggctgctg ccgctgctgg cggtttcggc atacctgcag gcgcagacgg
    13921 aagtcaggct ggcggtgcat aagtcgttca gcctgcccaa agggctgatt gcgcgcttcg
    13981 agcgggcaaa cgatgcgaaa gtgtcgatta ttcaggcggg cggcgcgaac gaaatgctca
    14041 acaaactgat tttgagccga gccaacccga ttgccgacgc ggtgtacggt ttggacaacg
    14101 ccaatatcgg caaggcgcgg gaaatgggca ttttggcggc ggcgcaaccc gaatccgccc
    14161 ccgtcgcggt cgggctgcct tcggctttgg cggtcgatta cggctatgtg tccgtcaatt
    14221 acgacaaaaa atggtttgaa ggcaaaaagc tgcccctgcc gcaaaccctg caggatttga
    14281 cccgccccga atataaaaac ctattggtcg tgccgtcccc cgccacatct tcccccggct
    14341 tgggcttcct gatggcgaac atcggcggtc tgggcgaaga aggcgcgttt aaatggtggg
    14401 cacagatgcg gcagaacggc gtgaaggtcg ccaaaggctg gagcgaggcg tattacaccg
    14461 acttttcgca caacggcggc gcgtatccgc tggtggtcgg ttatgccgcc agccctgcgg
    14521 cggaagtata tttttccaaa ggcaaataca gcgagccgcc gacgggcaac ctgtttttaa
    14581 aaggcggcgt attccgtcag gtcgaaggcg cggcggtctt gaagggcgcg aaacagccga
    14641 agctggcggc aaaactggtg caatggctgc aaagtctgga agtgcagcag gcggttccgt
    14701 ctgaaatgtg ggtttacccc gccgtcaaaa acacgcgcct gcccgacgtg ttccgcttcg
    14761 cccaagcccc ggcgcacact accgcccccg cgcagcgcga tattgatgcg aaccagcgcg
    14821 gatgggtttc ccgttggatc aggacggttt tgaaataaaa caaacatacc gccccgcaga
    14881 gcttcagacg gcatttttac acctgtgccg attacgccgc acggggcgga tgttcgatcg
    14941 agaggaaaac aatggacttc aaacaatttg attttttaca cctgatcagt gtttccggtt
    15001 gggggcatct ggctgaaaag gcgtgggcgt tcgggctgaa ccttgccgcc gcgctgctta
    15061 ttttcttggt cgggaaatgg gcggcgaaac gcattgtcgc cgtgatgagg gcggcgatga
    15121 cgcgcgcgca ggtcgatgcc acgctgatta gttttttgtg taatgttgcc aatatcggct
    15181 tattgatttt ggtgattatt gccgcattgg gcagattggg cgtttccaca acatccgtaa
    15241 ccgccttaat cggcggcgcg ggtttggcgg tggcgttgtc cttaaaagac cagctgtcca
    15301 attttgccgc cggcgcgctg attatcctgt tccgcccgtt caaagtcggc gactttatcc
    15361 gtgtcggcgg ttttgaagga tatgtccggg aaatcaaaat ggtgcagact tctttgcgga
    15421 cgaccgacaa cgaagaagtc gtgctgccca acagcgtggt gatgggcaac agcatcgtca
    15481 accgttccag cctgccgctt tgccgcgccc aagtgatagt cggcgtcgat tacaactgcg
    15541 atttgaaagt ggcgaaagag gcggtgttga aagccgccgc cgaacacccc ttgagcgttc
    15601 aaaacgaaga gcggcagccc gccgcctaca tcaccgcctt gggcgacaat gccatcgaaa
    15661 tcacattatg ggcttgggca aacgaagcag accgctggac gctgcaatgc gacttgaacg
    15721 aacaagtggt cgaaaacctc cgcaaagtca atatcaacat cccgttcccg caacgcgaca
    15781 tacacatcat caattcttaa acgccgtctg aaaggggggt gggaaatgga cgcgttgcac
    15841 accatcgccc gaaacctgac gaaaaaacgt caaaccgtaa gctgtgccga atcctgcacg
    15901 ggcggaatgc ttgccgccgc attcacaagc gttgcaggca gttcgcaatg gttcgaccag
    15961 agttttgtaa catacagcaa caaagccaaa gaataccgct tgggcgtgtt gcccgaaacc
    16021 ctgctcgaac acggcgcggt cagccgccaa accgtctatg agatggcgcg cggcgcgaaa
    16081 gccgtggctc aggcggatta cgccgtcggt atttccggca tcgccggccc gggcggcggc
    16141 ggcgaaagca aacccgtcgg cacggtttgg ttcgggtttg cctttccggg cggaagttgc
    16201 gaagcaatgc gccgttttga cggcaaccgc gaatccgtcc gcgcgcaggc ggtcgccttc
    16261 gcgttggaac ggttggccgg gctggttgaa aacggcggcg atgctgtctg aacaaatccc
    16321 cgccggataa aaaatgccgt ctgaaacgtt gcaggtttca gacggcattt tgtcgggtta
    16381 ggcggcggtg cggcttattt cactttgccc ttcaacgcgc cgtagcctgc cgcgtccatt
    16441 tgttccagcg ggatgaattt caagctcgcg ccgttgatgc agtagcgcag tccgcctttg
    16501 tcgcgagggc cgtcggggaa gacgtgtccc aagtgcgaat cggcggcgcg gcttctgact
    16561 tcggtgcggc gcatattgaa gctgaaatca tcgtgttcgg taacggattt tgcatcaatc
    16621 gggcgcgtga agctcggcca gccgcagccg gaatcatatt tgtcggcgga gctgaacagg
    16681 ggttcgccgc tgacaacgtc cacataaatg ccgggtttga acaaatggtc gtattcgtgg
    16741 ctgaaggcgt attcggtcgc gctgttttgg gtcacttggt attgctcttc ggtcagggtg
    16801 cgtttgagtt cggcgtcact cggtttttta tacgttgccg cgtcgaagcc tttgccttgc
    16861 ggtgcggctt tggttttgcc cggcagcggt tcgtcggctt tgcggatgtc gatgtggcag
    16921 tagccgttgg ggtttttaat caggtagtcc tgatggtatt cctcggcgtc gtagaagttt
    16981 ttcagcggtt cgttttcaac aacgaggggc agttggtatt tttgctgctc gcgtttgagg
    17041 gcggcggcga tgacggcttt ttcggcgggg tcggtgtagt acacgccgct gcggtattgc
    17101 gtgccggtgt cgttaccctg tttgttgagg ctggtcggat caacgacgcg gaaataatat
    17161 tgcaggatgt cgtccaggct gagtttgtcg gcatcgtagg tcactttgac ggtctcggca
    17221 tggcccgtat ggcggtagga cacgtcttcg tagctcgggt tttccgtgtt gccgttggcg
    17281 tagccggata ccgcgtcaac cacgccgtcg atgcgttgga aataggcttc caagccccag
    17341 aagcagccgc cggcgaggta gatggtgcgc gtgttcatga ttgctgaatc ctttttctga
    17401 gtgtcgggtt tgtagaacga atgtttcaaa ctgcccaaat cggcattcgg gttgcggatt
    17461 aacgccaatg cctgcgcttc gttgatgctg cctttgacga tgcgctgcac gtcgccgtct
    17521 ttaccgatta acgcccaaga aggataaacg ctgatattca ggttttgggc gatcgtgccg
    17581 ccgttgtcgg taacgacggg cagcttgggg tagttcaaac cggcatacca tttttgaaac
    17641 tcgccgtctt ttttctcgtg caaaaagccg ggggaggcga cggtaatcag gttggcggag
    17701 ctgaattttg catcttgcgc ccatttctcg gcctgtccca attcggacaa acataaagga
    17761 caccagctcg cccaaaattt aatcagcgtc ggtttgtctt ttttcaaata aacactggcg
    17821 gggcggttgt ccgcggtttt taacgtggat aaagtgtgcg gcacggtcgc ggtcccggca
    17881 tcgacgattt tgggcgaaca agcgcccagc gcaagcaggc agccgaactt ggcgcaaagg
    17941 gaaaagaaag tacggtgttt cattttgatg tttcctgtgt ggacggtttg catgattaga
    18001 cgtttgagat gccgaaacct tacggcccgg attttcagac aaccttgccg cgcaaaatac
    18061 gctacaatac gccctatttc aagtttctaa aattaaaagg aaaattcaat gttcagcttc
    18121 ttccgtcgca agaaaaaaca ggaaacgccg gctctcgagg aggcccaagt tcaggaaacc
    18181 gcagcaaaag tagaatctga agttgctcaa atagttggaa atattaaaga agatgtcgaa
    18241 tctttagcag aaagcgtcaa agggcgggcc gaatctgccg ttgaaaccgt cagcggtgcg
    18301 gttgaacagg taaaggaaac cgttgccgag atgccgtctg aagcagggga agcggcggaa
    18361 cgcgttgaat ccgcaaaaga agctgttgcc gaaaccgtcg gcgaggctgt cgggcaagtt
    18421 caagaagccg ttgcgacaac tgaagaacac aagctcggtt gggctgcgcg tttgaaacaa
    18481 ggcttggcca aatcacgcga caaaatggcg aaatcgctgg ccggcgtgtt cggcggcgga
    18541 caaatcggcg aggatttgta cgaagagctg gaaaccgtgc tgattaccgg cgatatgggc
    18601 atggaggcca ccgaatacct gatgaaagac gtgcgcggcc gcgtcagcct caaagggctg
    18661 aaagacggca acgaattgcg cggcgcgttg aaagaagcct tgtacgacct gattaagccg
    18721 ttggaaaaac cgctggtctt gcccgaaact aaagagcctt tcgtgattat gcttgccggt
    18781 atcaacggcg cgggcaaaac cacgtctatc ggcaaactcg ccaaatattt ccaagcgcag
    18841 ggcaaatccg tattgctggc ggcgggcgat accttccgcg ccgccgcccg tgagcagctt
    18901 caggcttggg gcgggcgcaa caatgtaacc gtcatttcac aaaccacggg cgattccgcc
    18961 gccgtgtgct tcgatgccgt ccaagccgcc aaagcgcgcg ggatcgacat cgtgcttgcc
    19021 gacaccgccg gccgcctgcc cacgcagctt catttgatgg aagaaatcaa aaaagtgaag
    19081 cgcgtgctgc aaaaagccat tcccggcgcg ccgcacgaaa ttatcgtcgt actcgatgcc
    19141 aatatcgggc aaaacgccgt caaccaagtc aaagcctttg acgacgcatt ggggctgacg
    19201 gggcttatcg ttaccaaact cgacggcacg gcaaaaggcg gcatcctcgc cgcgcttgct
    19261 tccgaccgcc ccgtccccgt ccgctacatc ggcgtgggcg aaggcataga cgacctgcgc
    19321 ccgtttgacg cgcgcgcgtt tgtggacgca ctgctggatt gagccgaaat gccgtccgaa
    19381 aacggcagac cgaaccgtca ttcccacgga agtgggaatc taggacgcgg ggtttgggca
    19441 actgttttta tccgataagt ttccgtgcgg acagatctag attcccgcct gcgcgggaat
    19501 gacgggtttc gagattgcgg tgttgtcgga cgggtttcga gattgcggtg ttgtcgggaa
    19561 tgacggttcg ggtattttac tgcgcccgcc ccgcgcctgt aaacggcggg cgcatcaaaa
    19621 atgccgtctg aaggttcaga cggcatcggt atcggggaat cagaagcggt agcgcacgcc
    19681 caatgaggct tcgtgggttt tgaagcgggt gttttccaag cgtccccagt tgtggtagcg
    19741 gtatccggcg tccagggtca gcttgggcgt gatgtcgatg cctacgcctg ccactgcgcc
    19801 gaagcccaag ctgctgatgc tgcggctttc gtgatgggga agtttgcggg tcggagcgtc
    19861 tttgataatg cctggctttt caacgccaag tgttttactg agtacaaacg tggtttcttt
    19921 ttccactgaa cgaacctgat gtttaacgtg tccgtaggcg acgcgcacgc ctgcataggg
    19981 tttgaagcgg gaaccggtat
//
LOCUS       DAXFWM010000041        19758 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000041 DAXFWM010000000
VERSION     DAXFWM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19758
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.041"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(114..1256)
                     /locus_tag="ACFI8B_001328"
     CDS             complement(114..1256)
                     /locus_tag="ACFI8B_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC3834541.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLEAADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1561..1824
                     /gene="rpsT"
                     /locus_tag="ACFI8B_001329"
     CDS             1561..1824
                     /gene="rpsT"
                     /locus_tag="ACFI8B_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC3834542.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1984..3132
                     /locus_tag="ACFI8B_001330"
     CDS             1984..3132
                     /locus_tag="ACFI8B_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC3834543.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3274..3975
                     /locus_tag="ACFI8B_001331"
     CDS             3274..3975
                     /locus_tag="ACFI8B_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC3834544.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4033..5841
                     /gene="aspS"
                     /locus_tag="ACFI8B_001332"
     CDS             4033..5841
                     /gene="aspS"
                     /locus_tag="ACFI8B_001332"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC3834545.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGIAGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLHLRQKAVETKEA"
     gene            5991..6275
                     /locus_tag="ACFI8B_001333"
     CDS             5991..6275
                     /locus_tag="ACFI8B_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC3834546.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6442..8334
                     /gene="speA"
                     /locus_tag="ACFI8B_001334"
     CDS             6442..8334
                     /gene="speA"
                     /locus_tag="ACFI8B_001334"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC3834547.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8433..9356
                     /gene="speB"
                     /locus_tag="ACFI8B_001335"
     CDS             8433..9356
                     /gene="speB"
                     /locus_tag="ACFI8B_001335"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC3834548.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9554..11020
                     /locus_tag="ACFI8B_001336"
     CDS             9554..11020
                     /locus_tag="ACFI8B_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC3834549.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11224..12300)
                     /locus_tag="ACFI8B_001337"
     CDS             complement(11224..12300)
                     /locus_tag="ACFI8B_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFC3834550.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12364..12501
                     /locus_tag="ACFI8B_001338"
     CDS             12364..12501
                     /locus_tag="ACFI8B_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC3834551.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            12523..13665
                     /gene="bioF"
                     /locus_tag="ACFI8B_001339"
     CDS             12523..13665
                     /gene="bioF"
                     /locus_tag="ACFI8B_001339"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC3834552.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13675..14322
                     /locus_tag="ACFI8B_001340"
     CDS             13675..14322
                     /locus_tag="ACFI8B_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC3834553.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14310..15092
                     /gene="bioC"
                     /locus_tag="ACFI8B_001341"
     CDS             14310..15092
                     /gene="bioC"
                     /locus_tag="ACFI8B_001341"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC3834554.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15313..16750
                     /locus_tag="ACFI8B_001342"
                     /pseudo
     CDS             15313..16750
                     /locus_tag="ACFI8B_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            16753..17851
                     /locus_tag="ACFI8B_001343"
                     /pseudo
     CDS             16753..17851
                     /locus_tag="ACFI8B_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17863..18398
                     /locus_tag="ACFI8B_001344"
                     /pseudo
     CDS             17863..18398
                     /locus_tag="ACFI8B_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            18400..19042
                     /locus_tag="ACFI8B_001345"
                     /pseudo
     CDS             18400..19042
                     /locus_tag="ACFI8B_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            19063..19422
                     /locus_tag="ACFI8B_001346"
     CDS             19063..19422
                     /locus_tag="ACFI8B_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC3834555.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19514..19589
                     /locus_tag="ACFI8B_001347"
     tRNA            19514..19589
                     /locus_tag="ACFI8B_001347"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19547..19549,aa:Asn,seq:gtt)
BASE COUNT         4964 a         4623 c         5095 g         5076 t
ORIGIN      
        1 atgtccgcaa tctgaaatcc cggccgtggg ggcgacggta taaatatttc tatttcaatc
       61 caatataaaa tgccgtccga acatcgttcg gacggcattt ttaatgcttc gaatcagttg
      121 gtgccgactt tgattttctg ccacaggctg accgacagtt ttttcgcatc cgtgctcatt
      181 tgcggcatga cgaaaccgtc tttcatatcc tgctcgttcg ggaagatgga acgggtgttc
      241 accaactcgg caggcatttt ttcgcgcgcc ggtttgctgg cgggggcgaa ggttacggcg
      301 atgccgtttt tcgccgcgat ttcggggtcg agcgtgtagt tgatgtattt gtgggcgttg
      361 gcgacgtttt tcgcatcggc gggaatcagc caagactcaa tccagaagcc catacctttc
      421 ggtgtcagca cttcgatgcc gacgttgttt ttcacttctt cggaacgcgc tttcgccaag
      481 ttcaaatcgc cgccgttgcc tgccgccagg cagatgtcgc cgcgcgccag ctcgtcgatg
      541 atggacgggc tgaaacgttt gacgtccgga cggatagact tcaacacttc cgccgccgcc
      601 ttcaaatctt caggattcga gcctttgggg tctttgccca agtagttcag caaaatcggg
      661 aacatttcgc tcggggtgtc ccacagggcg atgccgcagg atttcagctt gcgggtgtat
      721 tcgggtttga acagcaaatc ccagccgttt tcgggcagct tgccgcccaa aagctctttg
      781 cccttcgccg taatcgcaac cgtgttcacg ccggagaaat aggggacggc atactggttg
      841 cccgggtcgg cggcttccag cattttcaag agttcgggat cgatgttttt atagttggga
      901 atcaggtctt tgttgacttt ttgatacgcg cccgcctcga tttggcgcgg caggaaggcg
      961 atgcccggca cgaccaaatc gtaaccggat ttgccggtca gcattttggc ttccagcgtt
     1021 tcgttgtttt cgtacaagtc gtaagtcagc ttcagattgt tggctttttt aaagtcttcg
     1081 accgtactct cgtcaacata attcgaccag ttgtagatgt tcagagtatc ggtggcagcg
     1141 gcttcggcat tggcagcagg tgcgctgcct gcttgaggct gcacggcgtt tttttcgctg
     1201 ccgccgcagg ctgccagaga cagcgcggcc aaaacggcta atacggattt tttcatacgg
     1261 gcagattcct gatgaaagag gttggaaaaa aagaaaaccc cgcgccccat aaacaccccg
     1321 gcgcaaggtt tgggcattgt aaagtaaatt tgtgcaaact caaagcgata ttgacctgat
     1381 tttcctaaaa aattaccctg tttccaaaaa agggggggga aacggccgcc cgattttgcc
     1441 gtttttttgc gccgtcaggg tgtccgacgg gcggatagag aaaaaaggct tgcatataat
     1501 gtaaaccccc tttaaaattg cgcgtttaca gaatttattt ttctttcagg agattccaat
     1561 atggcaaaca gcgcacaagc acgcaaacgt gcccgccagt ccgtcaaaca acgcgcccac
     1621 aacgctagcc tgcgtaccgc attccgcacc gcagtgaaaa aagtattgaa agcagtcgaa
     1681 gcaggcgata aagctgccgc acaagcggtt taccaagagt ccgtcaaagt catcgaccgc
     1741 atcgccgaca aaggcgtgtt tcataaaaac aaagcggctc gccacaaaag ccgcctgtct
     1801 gcaaaagtaa aagcactggc ttgatttttg caaaaccgcc aaggcggttg atacgcgata
     1861 agcggaaaac cctgaagccc gacggtttcg gggttttctg tatttcgggg gtaaagttcg
     1921 aaatggcgga aagggtgcgg ttttttatcc gaatccgcta taaaatgccg tttgaaaacc
     1981 aatatgccga caatgggggc ggagatgaat acacgaaata tgcgctatat tcttttgaca
     2041 ggactgttgc cgacggcatc cgcttttgga gagaccgcgc tgcaatgcgc cgctttgacg
     2101 gacaatgtta cgcgtttggc gtgttacgac aggatttttg cggcacagct tccgtcttcg
     2161 gcagggcagg aagggcagga gtcgaaagcc gtactcaatc tgacggaaac cgtccgcagc
     2221 agcttggata agggcgaggc ggtcattgtt gttgaaaaag gcggggatgc gcttcctgcc
     2281 gacagtgcgg gcgaaaccgc cgatatctat acgcctttga gcctgatgta cgacttggac
     2341 aaaaacgatt tgcgcgggct gttgggcgta cgcgaacaca atccgatgta ccttatgccg
     2401 ttttggtata acaattcgcc caactatgcc ccgagttcgc cgacgcgcgg tacgactgta
     2461 caggaaaaat tcggacagca gaaacgtgcg gaaaccaaat tgcaggtttc gttcaaaagc
     2521 aaaattgccg aaaatttgtt taaaacccgc gcggatctgt ggttcggcta cacccaaaga
     2581 tccgattggc agatttacaa ccaaggcagg aaatccgcgc cgttccgcaa tacggattac
     2641 aaacctgaaa ttttcctgac ccagcctgtg aaggcggatt tgccgttcgg cggcaggctg
     2701 cgtatgctcg gtgcgggttt tgtccaccag tccaacggac agagccgtcc cgaatcgcgt
     2761 tcgtggaaca ggatttatgc catggcaggc atggaatggg gcaaattgac ggtgattccg
     2821 cgcgtgtggg tgcgtgcgtt cgatcagagc ggcgataaaa acgacaatcc cgatattgcc
     2881 gactatatgg ggtatggcga cgtgaagctg cagtaccgcc tgaacgacag gcagaatgtg
     2941 tattccgtat tgcgctacaa ccccaaaacg ggctacggcg cgattgaagc cgcctacacg
     3001 tttccgatta agggcaaact caaaggcgtg gtacgcggat tccacggtta cggcgagagc
     3061 ctgatcgact acaaccacaa gcagaacggt atcggtatcg ggttgatgtt caacgactgg
     3121 gacggcatct gaataccgtt tcaaacggca tatcaagttg ccgtgccgtc tgaagccgcc
     3181 ggcggtttgg cggggcggga taaaaaattc gtttgaatcc gggcaaacgc gtataatgtg
     3241 cggtttgtac ggactttgtc ggaatcaagc aagatgacgg aacctgcggc cgaaggcggc
     3301 aaagctgcca aggcgttaaa aaaatatctg attacaggca ttttggtctg gctgccgatt
     3361 gcggtaacgg tttgggtggt ttcctatatc gtttccgcgt ccgaccagct tgtcaacctg
     3421 ctgccgaagc aatggcggcc gcaatatgtt ttggggttta atatccccgg gctcggcgtt
     3481 attgttgcca ttgccgtatt gtttgtaacc ggattatttg ccgcaaacgt gttgggccgg
     3541 cagattcttg ccgcgtggga cagcctgttg gggcggattc cggttgtcaa atccatctat
     3601 tcgagtgtga aaaaagtatc cgaatcgctg ctgtccgaca gcagccgttc gtttaaaacg
     3661 ccggtactcg tgccgtttcc ccaatcgggt atttggacaa tcgcattcgt gtccggtcag
     3721 gtgtcgaatg cggttaaggc cgcattgccg caggatggcg attatctttc cgtgtatgtc
     3781 ccgaccacgc ccaacccgac cggcggttac tatattatgg taaagaaaag cgatgtgcgc
     3841 gaactcgata tgagcgtgga cgaagcgttg aaatatgtga tttcgctggg tatggtcatc
     3901 cctgacgacc tgcccgtcaa aacattggca ggacctatgc cgcctgaaaa ggcggagttg
     3961 cccgaacaac aataaagccg ccgttcagac ggcattttct gttttcagtt taaatcaata
     4021 aaaggtgatt ttatgcgtac caactattgc ggcctgatca gcgagcaata cttaggccaa
     4081 accgttaccg tcaaaggctg ggtacaccgc cggcgcgacc acggcggtgt gatttttatc
     4141 gacctgcgcg accgcgaagg catcgtccaa gtcgtgatcg atcccgacac gcccgaagcg
     4201 tttgccgctg ccgattccgc ccgtaacgaa tacgttttgg gcattgccgg ccgcgtacgc
     4261 aaccgtcccg aaggcacgac caacgacaaa atgatttccg gcaaaatcga gattcttgcc
     4321 aaagaaatcg aagtgttgaa cgccgccgct acgccgccgt tccaaatcga cgacgaaaac
     4381 atcagcgaaa acgtgcgcct gaccaaccgc gttatcgacc tgcgccgccc tgtgatgcag
     4441 cgcaacctgc gcctgcgtta ccaagtcgct atgggcgttc gccgttatct ggacgcacaa
     4501 ggcttcatcg acatcgaaac gccgatgctg acccgctcca ctccggaagg cgcgcgcgac
     4561 tacctcgtac cgagccgcgt tcatccgggc gagtttttcg cgctgccgca atcgccgcaa
     4621 ttattcaaac aactgttgat ggtggcgggt ttcgaccgtt actaccaaat caccaagtgc
     4681 ttccgtgacg aagacttgcg cgccgaccgc caacctgaat tcactcaaat cgacttggaa
     4741 acctcgttcc tgaacgagga tgaaatcatg gacatcactg aaggcatggc caaacaagtc
     4801 ttcaaagatg ctttgaacgt agatttgggc gacttcccac gcatgcctta ttctgaagcc
     4861 atgttctact acggctctga caaaccggac atgcgcatca acttaaaatt caccgagttg
     4921 accgacctga tgaaaacgga agaattcaaa gtcttccgtg gcgcagccga catgaaaggc
     4981 ggccgcgtag tcgctctgcg cgtgcctaac ggcgccaaat tcagccgcaa agaaatcgac
     5041 gaatacacta aatttgtcgg catctacggc gcgaaaggtc tggcatacat caaagtaaac
     5101 gatgcgggca acctctccaa cggcgaagac agcggcctgc aatctccaat cgtaaaattc
     5161 ttgtctgaaa acgccctgaa agaaatcatc gagcgtaccg ccgcgcaaaa cggcgacatc
     5221 atcttcttcg gtgcggacaa agccaaagtc gtgaacgaag ccgtcggcgc attgcgtatc
     5281 aaagtcggct tggaacacgg caaagacaac ggctacttca cagacgaatg gaaacctttg
     5341 tgggttgtcg atttcccgat gttcgaatac gacgaagaag ccgaccgcta cgttgccgta
     5401 caccatccgt ttaccgctcc taaagaaggt catgaagacc tgatggtttc cgatcctgca
     5461 aactgcctgg cccgtgccta cgatatggta ttgaacggct gggaaatcgg cggaggctct
     5521 atccgtatcc accgtgcaga cgtacaagag aaagtgtttg ccgcgctgaa aatcagccct
     5581 gaagagcaac aagagaaatt cggcttcctc ttggacaacc tgaaattcgg tgcgcctcct
     5641 cacggcggcc ttgcattcgg cctcgaccgt ctggtaacgc tgatgaccgg tgccgaatcc
     5701 atccgcgacg tgattgcctt cccgaaaaca caacgcgccc aatgcctgct gaccgatgcg
     5761 cccaacagcg tggacgataa gcagttgcgt gaattgagcc tgcatctgcg tcaaaaagca
     5821 gttgaaacaa aagaagcata aggaaaacag agccgtttga cggctctgtt ttttcagacg
     5881 gcatttacgc ttcttgactt ccctctaatt caaacctaat tttgctgtgt tttaatggcg
     5941 gtattgaaaa acacatcttg ttcaatcaac cgataaaaaa aggactgaaa atgaaaaaac
     6001 tgttattggc cgccgttgtt tccctaaatg ccgcaaccgc atttgccggc gactctgccg
     6061 agcgtcagat ttacggcgat ccccattttg aacaaaaccg cacaaaagcc gtgaaaatgt
     6121 tggaacagcg cggttatcag gtttacgatg tcgatgccga cgactactgg ggcaaacctg
     6181 ttttggaagt ggaagcctat aaagacggcc gcgaatacga catcgtgttg tcttaccccg
     6241 acctgaaaat catcaaagag cagctcgatc gctgactcct ttgatggaaa gatgaaccaa
     6301 aatgccgtct gaagcgttca gacggcattt tgcctgttcc tcatcaggta tgaggcaggc
     6361 ttttcttatt aaaaaaatga catttcacgc tgatttgtta taatcattcc ttttcaacac
     6421 gacagacgga gcaggtttat tatgcctatc cttaccatcc gtgaagtgtg caacattaat
     6481 cattggagca taggttatta tgatgttgac gattccggcg aaatcatcgt ccgccccaat
     6541 ccctcgcaac acaatcaaac tgtttcactg caaaaactga ctgaagccgt gcaacaaaaa
     6601 catcaggcgc gcctgcctgt tttgttttgt tttccgcaaa tcctcgaaca ccgcctccgc
     6661 gacattaacc gcgcctttca gacggcacgg gaagagtgcg gctataaggg cggttattgt
     6721 ttggtttacc ctatcaaggt caaccaacac cgccgcgtca tcgagtcgct tatgtcaagc
     6781 ggacaaccgc atggtttgga agccggttct aaagccgaac tgatggcggt tttggcacac
     6841 gccggcaccc ggcaaacatt aatcgtctgc aacggctata aagaccgtga atatatccgt
     6901 ttcgccttga tgggcgaaaa actggggcat caggtttatt tggtgattga gaagctgtcc
     6961 gaaatacaaa tggtattgga agaggcggaa aaactcggca tcaagccccg tttgggtgtg
     7021 cgcgccagac tggcttccca aggttcggga aaatggcagt cttcgggtgg ggaaaaatca
     7081 aaattcggct tgtcggcttc ccaagttttg caactggtcg atattttgaa acaaaaaaac
     7141 aggctggact gcctgcagct tttgcatttc catttgggct cgcagcttgg gaacatccgt
     7201 gatgttgcca caggtgtaca cgaatcggct cggttttatg ttgagttgca caaactgggg
     7261 gtaaatatcc gctgttttga tgtaggtggc gggcttggcg tggattacga aggaaaccgc
     7321 acacaatcgg attgttccgt taattacagc ctgaacgaat atgccgccac agtcgtatgg
     7381 ggcatcagtc aggcttgtct cgaacacggg ctgccgcatc cgacaatcat taccgagagc
     7441 gggcgcggca ttaccgcaca tcacgccgtt ttggttgcta atgttatagg cgttgaacgt
     7501 tacaaaccgc gtcggctgga tgcgccatcg cccgaagcac cgcgtgtgtt gcacagtatg
     7561 tgggaaactt ggacggatat ttccgcctcg cgggaaaaac gttccttacg cagctggata
     7621 cacgaagggc agtttgatct tgccgatgtg cataatcagt ataatgtcgg gctgttgagt
     7681 ttggcgcaac gtgcgtgggc ggagcaactg tatttaaata tctgtcatga agtcggcgaa
     7741 ttgtttaatg aaaaacaccg gtctcaccga accattattg acgaattgca agaacgtttt
     7801 gccgataagc tgtatgtcaa tttctcactc ttccaatctt tgcccgatgc ttggggcata
     7861 gatcaacttt tccctgtttg tcctattacc ggtttgaatg aaccgattgc gcgccgcgcc
     7921 gtgttgttgg acattacctg cgattcagac ggtacgattg accactacat cgacggcgac
     7981 ggcatcgccg gtacgatgcc tatgcctgat tatcccgaag aagagccgcc gtttttaggc
     8041 ttttttatgg tgggagcata tcaggaaata ctcggcaata tgcacaatct tttcggcgac
     8101 actgccactg ctgatgttgt tgtaagggaa gacggacaat ttaccgtcat cgattacgat
     8161 gaaggaaaca ccgttgccga tatgctcgaa tacgtttatc aagatccgaa agagctgatg
     8221 aaacgctatc gcgaacaaat cgaacattca gaccttcctg cctcgcaggc tatgtctttc
     8281 ttaaaagaac tcgaagcggg gcttaatggt tatacctatt tggaagacga atagacgcat
     8341 caaggcatcg gatatgtcgt ctgaagcccg attttcttac tcaaacacca atcatcacga
     8401 ccgattgaaa ccaattacaa ggaatcatta cgatgcaata cagcacactg gcaggacaaa
     8461 ccgacaactc cctcgtttcc aataatttcg ggtttttgcg cctgccgctt aattttatgc
     8521 cgtatgaaag ccatgccgat tgggttatta ccggcgtgcc ttatgatatg gcggtttcag
     8581 ggcgttccgg cgcgcgtttc ggtcctgaag ccatccggcg cgcctccgtc aacctcgctt
     8641 gggagcaccg caggtttccg tggacatttg atgtgcgcga acgcctgaac attattgatt
     8701 gcggcgactt ggttttttct tttggcgaca gcagggattt tgtcgaaaaa atggaagcgc
     8761 acgccggcaa attactttct ttcggcaaac gctgtttgag tttgggcggc gaccatttca
     8821 ttaccctccc gttgttgcgc gcccacgccc gctatttcgg caaactcgca ctgattcatt
     8881 ttgacgcgca caccgacacc tacgacaacg gcagcgaata cgaccacggc acgatgtttt
     8941 ataccgcccc caaggaaggc ctcatcgacc cgtcccgttc cgtacaaatc ggcatacgca
     9001 ccgaacacag taaaaaattg ccttttactg tgttgtccgc ccccaaagtc aatgaagaca
     9061 gtgttgaaga gaccgtccgt aaaatcaaag aaaccgtcgg caatatgccc gtttacctga
     9121 ctttcgacat agactgtctc gacccgtcgt tcgcccccgg gaccggtacg cccgtatgcg
     9181 gcggcttgag cagcgacagg gcattaaaaa tcctacgtgg gctgacggat ctcgacatcg
     9241 tcggtatgga tgttgtagaa gttgccccct cttacgacca atccgacatt accgctttgg
     9301 ccggcgccac aattgccttg gaaatgcttt accttcaagg tgcgaaaaag gactgaacgt
     9361 ccggcatccc ccgggttttc gccgtgccgt tcaaacggcg tattcagtct aatgaaaatt
     9421 caaatactga aacaaaagtt gcccggagcc gcatatcgga aagacggtga aatatcgtaa
     9481 tataacttat aaaacaatta gttaaatatt atttttccga tttttcggga cggtcttttt
     9541 tacggaggtc aatatgatga aattgggttt caaaccgata cccctcgcca ttgccgcagt
     9601 attgtgcgcc ctggttttgg cactgcccgt acccgacggg gtcaagcctc aggcttggac
     9661 gctgctggct atgtttgtcg gtgtgattgc cgccattatc ggcaaggtta tgccgttggg
     9721 cgcgctgtcg attattgccg tcgggttggt cgcagtaacc ggcgtaaccg ccgacaaacc
     9781 gggcgcggcg atgagcgatg cgttgagtgc gttcgccaat ccgttgattt ggctgattgc
     9841 catcgcagtt atgatttcgc gcggtttgct caaaacaggg ctggggatgc gtatcggata
     9901 tttgtttatc gccgtttttg gaagaaaaac gctgggcatc ggttacagtc tcgctctttc
     9961 cgaactgctg ctggctcccg ttaccccttc caataccgcg cgcggcggcg gcattataca
    10021 tccgattatg cagtcgattg ccggcagtta cggctccaat cccgcaaaag gcacagaagg
    10081 caagatgggt aaatatttgg ctttggtcaa ttatcattcc aatcccattt cgtcggctat
    10141 gtttattact gcaactgccc ccaacccttt aatcgtcaac ttgattgccg aaaatttagg
    10201 cagtagtttc cgtctttctt ggggggcgtg ggcgtgggca atggctgttc ccggcgttat
    10261 cgcctttttc gttatgcctt tgattttata ttttttgtat ccgcctgaaa ttaaagaaac
    10321 gcccaatgct gttcaatttg ccaaagaccg tctgagcgag atgggtaaaa tgtcggcaga
    10381 cgaaatcatt atggcggtca ttttcggtat cttgctgctg ttgtgggcag atgttcccgc
    10441 ccttattacc ggcaatcacg cttttagtat caacgccacc gccaccgcat ttatcggatt
    10501 aagcctgctt ttgctttccg gtgtattgac ttgggacgat gttttgaaag aaaaaagcgc
    10561 gtgggatacg attatttggt ttggcgcatt gattatgatg gccgcatttt taaataaact
    10621 cggactgatt aaatggttct ccggagtgtt ggcggaaagt gtcggcggtt tgggcgttag
    10681 cggcacggct gcgggcgtaa tcctcgtgct tgcttatatg tatgcgcatt atatgtttgc
    10741 cagtactact gcacatatta ccgctatgtt cggcgcattt ctcgctgctg ccgtttcact
    10801 gaatgccccg gcgatgccga ccgcgctgat gatggcggcc gcatccaaca ttatgatgac
    10861 cctcactcat tatgcgaccg gtacttcacc tgtgattttc ggctcgggct acaccacaat
    10921 gggagaatgg tggaaggcgg gttttatcat gagcgtagtc aattttctga ttttttccgt
    10981 tatcggcagc atttggtgga aagttctggg atattggtaa agggaaaaat aaatttccaa
    11041 tctgtgttta tttgattggg cgactattat cgtgaaatat gccgtctaaa gccttcagac
    11101 ggcatatttg tgcgcttgga tgttgcagaa agcggcaggg cggcggtgta ggaaaagcca
    11161 aacaaaaacc aaaccgcctg tcaacttctg ataaacataa gtattaaata atcagaaggt
    11221 tattcaatta cctaaacgca aattcccctg ccgtatcaca tctattgaaa ataatacatc
    11281 aaccggctcg gaagcagcct gatcaggtga ttctacttgc ggcgatgaat cggcagccgg
    11341 ttcggtatag gcagtcggcg tgccgtcgga ttgggtattt aaatgcgctt gtgcctcggg
    11401 cgtgtgtaca tcaggcactt cgggcgtgcg tgtctcggat acttcggcag agttggtttc
    11461 ctcagtttgt tcaatgactt cagcttggct gtatgaggaa gaaccctgta tccacgccag
    11521 cgatttgagc ggcatcttca tcttgccgtt tttgccgcag gtcagacaga cggccgatcc
    11581 ggtgcggtct ttgagtacgg catcgacttt ctcgggcgcg atggttttgc cctgtgtttt
    11641 tgcccattgc ccgatgcttt ttttcaggga ggcggtgtca aggttgttct taccgtaagg
    11701 gtcgcggaag gcggcaagcc acaggttgcg atccgcatct tcattgaggc ccgccatttg
    11761 atagatgacg gaggcgggcg agccggtggc gatggttttg gcaaactcga gcgcgtcggg
    11821 cgatttcatg cggacgcagc cgtgactccg gatgcccggg acgctggccg gtgcattggt
    11881 tccgtgtatg cccaaaccga gtttcgggtc tcctaagcgg acaaaaaccg gccccaaagg
    11941 gttgtccgga ccggcggcta tggtttttac gccgtcgccg cgttctttct gtatggattt
    12001 tgggatgtgc caaacagggt tataggcttt cgccccgatt ttatgttcgc ctagattggt
    12061 ttgtgtcatc gcccgaccta ctgcaacggg ataaaccttg gtcagtttgc cgtcggtgta
    12121 gaggaacagg cgttgctgag ggatgttaat gaagacatgt tgaccttgtg cgacggggga
    12181 gacatcggga atgacggtgt ttgcgtatga aaaaccgctt atcaatagtg cggcagtgcg
    12241 gcagattgtt ttattcatat caaaatatgg tgtgtgtccg ataggttttc ggcaaatcat
    12301 acctgaaacc gtaccaattt gtgcgaaaat atgcgcttcg gtacagtgcg gacggattgg
    12361 gtaatggcaa cggaaacaaa tatcgcggaa atttccgcct tggattatga aggcaggggt
    12421 gtggcaaagg tcggcggcaa aacgattttc attaaagggg cattacttga ttgtttgatg
    12481 ctgggttgct tcaggcttta actcaggaat atttacatca taatgaaagt ttttagacaa
    12541 cagcttgaac aactcggcgc gcaaaatcaa tatcgttcga ttccggattt gattcatcaa
    12601 gggcggtata ttacgcggga aaaccgcaaa atgctgaata tgtcgtctaa tgattatttg
    12661 gggttggcat cagatgaaaa cttacgccgg tcttttttgc agcaatacgg cggtaatttt
    12721 ccctctttta ccagttcttc atcacgttta ttaacgggca actttcctat ttataccgat
    12781 ttggaagagc ttgtcgcaca acgtttccaa cgggaaagcg cgttattgtt caacagcggc
    12841 tatcacgcca atctcggtat tttgcctgct ttgacgacga cgaaaagttt gattttggca
    12901 gataaattcg ttcacgccag tatgattgac ggcatccgtt tgagccggtg tgcgtttttc
    12961 cgttatcgtc ataatgatta tgaacatttg aaaaatctgc ttgaaaaaaa cgtcggaaaa
    13021 tttgaccgca cttttatcgt taccgaatct gttttcagta tggacggcga tgtggcggat
    13081 ttgaaacagc ttgtccaatt aaaaaaacag tttcccaata cttatcttta tgtggatgaa
    13141 gcccacgcaa tcggtgttta tgggcaaaac ggattgggga ttgccgaacg ggataatctg
    13201 attgccgaga ttgatttatt ggtcggcact ttcggtaaag ccttagcctc ggtgggggcg
    13261 tatgccgtct gcaaccaagt attgaaagaa tgtttgatta atcaaatgcg cccattgatt
    13321 ttttcaaccg cattgccgcc gtttaatgtg gcttggactt attttatttt tgaacgattg
    13381 ccgcaattct caaaagaaag aagtcatctt gagcagttaa gcgcattttt acggcgggaa
    13441 gtggcgcatc ggacgcaaat aatgccgagc gaaacctgta tcgtccccta tattttaggc
    13501 gggaatgaag ccacccttgc caaagcggaa tatctgcaag gtcagggtta ttattgcctg
    13561 cccatcagac cgccgacagt accaaaaaac acatccagaa tccgcctgtc tttaactgcc
    13621 gatatgacaa cggatgaagt gcggcagttt gcggcgtgcc tgtaaggata tgatatgaaa
    13681 acaaaatttt acaatcatca aggcgaacat ttaatcctgt attttgcagg ttggggaatg
    13741 ccgcccgatg ctgtaaatca tttgattttg ccggaaaatc acgatttatt gatttgctat
    13801 gattatcaag atttaaattt ggattttgat ttttccgcct atcggcacat ccgtttggtg
    13861 gcgtggtcaa tgggcgtttg ggcggcagag agggcattgc aaggaataag attaaaatcc
    13921 gcaacggcag tgaatggcac aggtttgcct tgcgatgata atttcggtat cccttgcgcc
    13981 gtttttaaag gcacattgga gaatctcaca gaaaatactc gttcaaagtt tgaacgtaga
    14041 atctgtggcg ataaagcatc ttttgaagat taccaacaat ttcccgcccg tccgtttgac
    14101 gaaattcatc aagaacttac cgcacttttt gcgatgatcg ggcaagatag gcgtacagat
    14161 cttatccgct ggacaaacgc cttgttcgga tcgggcgata aaatttttat tcctgccaat
    14221 cagcaccgat attggacacc gcgttgcacc gttcaagaaa cagacggcgg gcattacctg
    14281 ttttcaagat ttacccattg gtcggcacta tggaatcact gactgccata aataaaccgc
    14341 gcattcggca ggctttccaa aaagcattaa gcgattatga caggcacgcc ttaatccaac
    14401 aaaaaatgac gattaatcta atcgcgcatt tgcaagatta tttgccggat atgccattgg
    14461 aaaacgtgtt ggaattgggc tgcggctcag gaatgttgag tgccttgctg caaaaacaga
    14521 tttcagcgaa ttattggtta tttaatgatt tgtgcgatgt acgaagccga ctggctgaaa
    14581 aactgcctca atcctttgat ttttattgcg gcgatgcgga aaattttcct tttcaacgac
    14641 aatttgactt aatcgcaagc gcatctgccg tgcaatggtt tcaccaaccc gacgctttta
    14701 tcgcccattg caaaacaggc ttgaaaacaa acggattatt ggcggttgcg acctttggca
    14761 aagacaattt aaaagaagtc cgccaaatca caaatatagg cttaaattac ccgactttat
    14821 cccaatggca ggcttggtta gccaaagatt ttgagctttt atggtgtgag gattttaagg
    14881 taatactaga ctttgatacg ccgttagatg tactcaaaca ccttaaatat acaggcgtta
    14941 ccgccacgaa ccaaaaaaat tggacaagaa aaaatctcaa tggatttatt ggcgattact
    15001 tgtcggcgtt cggtatgccg tcgggcaaag tgcgcctgac ttatcatccg ttatttttta
    15061 tcgcgcgcca ctctgcggcg ggcaggcaat aaggcagctt atgggcaaag ttatttttat
    15121 atcgggtatt gatactgatg tgggtaaaag gtaatatggc gagccttatg cagaaggcat
    15181 attgtcaaac gttaaattat ggtatgattt aaaatttaca agtctatttc agtaaatcgt
    15241 taataataaa agcggacaat tggccgttgc gggcggtcag ctatccgctc gttacggtat
    15301 cgggggtttc ctttggaaaa ctcattaatt atcggtctgg cactggcggt attgctgatg
    15361 cttttggtta tgcgtgcgaa gcagggtaag aaaacaccga agcgcaaatc ccaaggtgtc
    15421 ggcaatacgc aggggcagcc gcccggaagc aatgattcgg actgggtcga tcagacttgg
    15481 tcaatcggtt tcagacggca cgcaactcga ctggtcttgg aacgaaagtg ccgagaccgc
    15541 atccgccgcc gtatccgcgc aagaagtcga tccgcttacg gagtatcagg tttataagca
    15601 attcggttat cagggcaagg ctgccgaatc tttggctgcc tatctggacg gcattccgga
    15661 tggtgaagcg aaacctgaga accttatccg cgagctgatc gatatcaatc tcgaagtggg
    15721 ggatgtcgat gttttggcag acaatctgca aaaatacggc agactgattc ctttcgaact
    15781 tttggcaaaa tatatcgaac aggcattaca gcgagattca aaccatttgc gtatccgcgt
    15841 cttggcggaa gaaggtttgg gatagggcac tcaggagatt gaaaaacgtg cggaaggcgg
    15901 ttctgcgacg gcagcttccg catcgccccc gccggatgtc ggcggtaagg cttatggagc
    15961 cgaagaaatc aagcgcatcc cgattgtgcg gggcaaaaaa gacgtgtccg gaatcagcca
    16021 agagaaaatc ggtgcgattg ccggcctggt ccgcgccgat caaggtgcga aaatccttaa
    16081 agacaaagtc agctacgaaa cggcatcgaa acaatacgac cgtgccatcc aaacttccga
    16141 aaaacctgca aacctgatta tcgatgcgtt gaaactcgat taccaacacg cggacataga
    16201 ccgttttgcc ggacatttgt ggaaacttta ccaaacgttg ggcaactacg gcaggcaggt
    16261 taaagagcgg atgctgggtt aggggtacag cctgggttac catgaagttt tcgatgattt
    16321 ggaaaagggg ccgaacgatc ggcaaatcaa agacatcggt atgaggcacg ggtatctgcc
    16381 gaaaaatata cagaaattca aagcgcaaca tcgggatttg gtgcttcaag attcttcgtt
    16441 gatcaacact ggttcgtctc cggcagacga tgcggttaaa gaagtagagt cgttgctgat
    16501 gtatggtcag attgaagcgg caatggatgt gttggagcag gcggtattga aatatcccga
    16561 cgaatcccag ctttatatta cgttgatcga tatttatgaa cgtactgaag attgggatag
    16621 gttggggcag tttttaaggg tattgaggga acgtgcggac aggcttcctg aagaggtcgt
    16681 tatgctgatg agccggctgc tgcagcgtat gaatcaaaat attaaaaaaa taaaacggta
    16741 cggaaaataa aaatggaagt tcaactgccg aaaattaaaa cagtacgcgt aatgttggcg
    16801 gggatgacgg cgcagcagga atccgttttc aaaatggcat tcaaaatgca caataccacc
    16861 cgttatgaaa cagtatcccc ttcagacggc agtgccgtgc ccgatttggt tttggcggat
    16921 accgatgccg agggcggttt tgaactttgg aaagagcttg ccgggcgtta taaggctata
    16981 cccgtcgccg tctgttcgga gaaagttccc gattctgaag ttccctacct gcccaaaccg
    17041 attcggtttg aaacattgtt tcctatgctc cgcaagttat tgcagggcga gaatgtttat
    17101 gggaaatcgt ttattgcacc cgcagaccgg tcggcgaaaa acaacgggaa tgtgcagcgt
    17161 acggttacga tacgccagtt taacccgaat aaaggattat tgggggcgtt gcggtttgcg
    17221 gaaaagaaca ggcaggacat cgctatcttg catggaaata agccggtcct taattgtttt
    17281 cccctcgata caacgggttt tgctgacaga aagtgtgtaa aaactcgaag aattgtgcaa
    17341 agacgaaaat ttgcaggtca gttgcaagac tgttcccgat aactcgcaat ggcgcgaaaa
    17401 ggctaaagta ggcattatgt cctgtatgtg gcagttttcc atttggacgg cgcaggacag
    17461 gttgatttat ccgatttttc ccgatactcc gtttacgttg aaatcttggc caaacctgac
    17521 ccggtaggca aatgtgccgg ggtcgatacg cttgtcggca tttttgacca aggcatccgt
    17581 caaccttaac gtgttgtata aagtgatgcc tttaaacctc aatgatattc tgaattatct
    17641 tgcggcaacc tatacaaccg ggtttttgtc ggtagatttg aaaacggttt cacaacaggc
    17701 atactccgat atggcggata aaataaatat cggggccgat tctgcttctg atagtgaaat
    17761 gatgaaaaaa gcggaaaaaa tcacgacacc atcccaatcc cagtcgcgcg gccttctgca
    17821 aagactgatg aaaaaactgt tgggcagcta agaggcggag agatgagaga aaataaaatt
    17881 attttcacag gacctgtcgg cgtagggaaa accactgcca ttgcggctat ttcggacgaa
    17941 gcactcgtcc agaccgatgc ttccgcatcc gacatgactt tggataggaa aaggaatacg
    18001 acagtggcga tggactacgg gccatcagct tggatgagga taccaaagtc catttatata
    18061 gtacgcccgg tcaggaacgg ttcaacttta tgtgggaaat cttaagccaa ggcagtatgg
    18121 gtttggtatt gcttttagat aatgcccgaa ccaacccgtt gaaagatttg gaattctttt
    18181 tacattcgtt tcgagggctg ctggagaagg catccgtcgt tgtcggtatt accaagatgg
    18241 atatacgctc tcagcccggt atcgacgtgt atcacaaata tcttgcaaaa cataatctta
    18301 atgttccggt ttttgaaatt gatgcccgta aggaagatga cgtaaaacaa ttggttagcg
    18361 caatgttatt ttctattgat ccgggactgg aggtttaata tggaatcaac actttcacta
    18421 caagcgaatt tatatccctg cctgactcct gccggtgcat tttatgccgt atccagcgat
    18481 gcccccagtg ccggtaaaac tttgttgcgc agcctgttga aagcggatgc ggacgaagtg
    18541 gtcagcagtg agaagctgct cgcgggcgga caccgccgac atcgataccg ctttgaacct
    18601 gttgtaccgt ttgcaaaaac tcgaattcct ctatggcgat gaaaacggtc attcagacgg
    18661 catcaatttg tcggacgagc aattgccgtt gctgatggaa caattgtccg gcagcggtaa
    18721 ggcattattg gtcgatcgga acggtctgta tcttgccaac gccaatttcc atcatgagtc
    18781 ggcggaagag ttggggttgt tggcggcaga agtcgcacag atggaaaaga aataccggct
    18841 gctgattagg aacaacctgt atatcaacaa taacgcttgg ggcgtttgcg atccttccgg
    18901 tcagagcgaa ttgacatttt tcccattgta tatcggttca accaaattta ttttggttat
    18961 cgccggcatt cccgatttga gcaaagaggc atttgttact ttggtaagga ttttataccg
    19021 ccgttacagc aaccgcgtgt aaaacttggg acaggaggga ttatgcagca attattgatt
    19081 tcaatccttg aagatttaaa taatacatct acggatatta tcgcgtctgc cgttatctca
    19141 accgacggat tgccgatggc gacaatgctt ccttcacatt tgaattcgga cagggtaggg
    19201 gcgatttctg ccactttgct tgctttgggg agtcgctcgg tgcaggaact cgcctgcggg
    19261 gaattggaac aagtgatgat taaaggaaaa tcaggctata tccttttaag tcaggcgggt
    19321 aaagatgccg tgttggtgct ggtggcaaaa gaaaccggca gacttggttt aatcctattg
    19381 gatgccaaac gtgcggcaag gcatattgcg gaagccatat aacatataaa gattgcgggc
    19441 ttgcagataa gtgcaatcga ttgtcaattt atattgacac gttcggtatt tctgttttat
    19501 tattcgcgtt tactccccga tagctcagtc ggtagagtga cggactgtta atccgcaggt
    19561 ccctggttcg agcccaggtc ggggagccaa attccaaaac cctctaagta ttttcttaga
    19621 gggttttgtt ttaccggcgg tcagaaacgc atttttgaga tgattgtttt gagatggaat
    19681 aaaatctttg caaaattcct ttcgtgatgg ttatgaaaaa taaatttcta aagatttaat
    19741 tggttatttt tataattt
//
LOCUS       DAXFWM010000042        19145 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000042 DAXFWM010000000
VERSION     DAXFWM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19145
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.042"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1259
                     /locus_tag="ACFI8B_001348"
     CDS             <1..1259
                     /locus_tag="ACFI8B_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC3834556.1"
                     /translation="PKKYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASA
                     EGSVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVAVGSEAKVNAERGIAIGKEAKA
                     GGKTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGD
                     FSTAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHS
                     VSLGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVND
                     FDAVNVRQSKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFG
                     TYKGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKV
                     LMEQLMQDNRQLKPRLEKLEARLNAM"
     gene            complement(1398..1913)
                     /gene="coaD"
                     /locus_tag="ACFI8B_001349"
     CDS             complement(1398..1913)
                     /gene="coaD"
                     /locus_tag="ACFI8B_001349"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC3834557.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2043..2726)
                     /locus_tag="ACFI8B_001350"
     CDS             complement(2043..2726)
                     /locus_tag="ACFI8B_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFC3834558.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2883..3149)
                     /locus_tag="ACFI8B_001351"
     CDS             complement(2883..3149)
                     /locus_tag="ACFI8B_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC3834559.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3223..3693)
                     /gene="rlmH"
                     /locus_tag="ACFI8B_001352"
     CDS             complement(3223..3693)
                     /gene="rlmH"
                     /locus_tag="ACFI8B_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC3834560.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYAFKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3746..4132)
                     /gene="rsfS"
                     /locus_tag="ACFI8B_001353"
     CDS             complement(3746..4132)
                     /gene="rsfS"
                     /locus_tag="ACFI8B_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC3834561.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4191..4796)
                     /gene="nadD"
                     /locus_tag="ACFI8B_001354"
     CDS             complement(4191..4796)
                     /gene="nadD"
                     /locus_tag="ACFI8B_001354"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC3834562.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPAAARYIRKHGLYEK"
     gene            complement(4793..5386)
                     /locus_tag="ACFI8B_001355"
     CDS             complement(4793..5386)
                     /locus_tag="ACFI8B_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC3834563.1"
                     /translation="MSELSEILSYNQKFVESGGYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDLTGFDNVEDSVRHTVDLIRNHPLMPR
                     HIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5415..6956)
                     /locus_tag="ACFI8B_001356"
     CDS             complement(5415..6956)
                     /locus_tag="ACFI8B_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3834564.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7347..8732
                     /locus_tag="ACFI8B_001357"
     CDS             7347..8732
                     /locus_tag="ACFI8B_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC3834565.1"
                     /translation="MDGRTQTLSAQTLLGISAAAIILILILIVKFRIRALLTLVIASL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFAPGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASVGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRAIHVPVP
                     ELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPVCPVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLAALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIAFIG
                     FALSALLFAIV"
     gene            8752..9270
                     /locus_tag="ACFI8B_001358"
     CDS             8752..9270
                     /locus_tag="ACFI8B_001358"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC3834566.1"
                     /translation="MTTHFVVMGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9456..10373)
                     /gene="thrB"
                     /locus_tag="ACFI8B_001359"
     CDS             complement(9456..10373)
                     /gene="thrB"
                     /locus_tag="ACFI8B_001359"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC3834567.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10410..11138)
                     /gene="ubiG"
                     /locus_tag="ACFI8B_001360"
     CDS             complement(10410..11138)
                     /gene="ubiG"
                     /locus_tag="ACFI8B_001360"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC3834568.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECVRVEDLAGAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVRPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFISPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11286..12527
                     /locus_tag="ACFI8B_001361"
     CDS             11286..12527
                     /locus_tag="ACFI8B_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC3834569.1"
                     /translation="MSAKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSGGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12679..13734
                     /locus_tag="ACFI8B_001362"
     CDS             12679..13734
                     /locus_tag="ACFI8B_001362"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3834570.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTEL
                     PVKLAVAGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVARAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDMLASV"
     gene            complement(13801..15453)
                     /locus_tag="ACFI8B_001363"
     CDS             complement(13801..15453)
                     /locus_tag="ACFI8B_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC3834571.1"
                     /translation="MVAYAFLFLFVTAVVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETQPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15596..16159
                     /gene="gmhB"
                     /locus_tag="ACFI8B_001364"
     CDS             15596..16159
                     /gene="gmhB"
                     /locus_tag="ACFI8B_001364"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC3834572.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSADEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16189..16932
                     /locus_tag="ACFI8B_001365"
     CDS             16189..16932
                     /locus_tag="ACFI8B_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC3834573.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16929..17617
                     /locus_tag="ACFI8B_001366"
                     /pseudo
     CDS             16929..17617
                     /locus_tag="ACFI8B_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
     gene            17699..17830
                     /locus_tag="ACFI8B_001367"
     CDS             17699..17830
                     /locus_tag="ACFI8B_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFC3834574.1"
                     /translation="MPPVRGRMAASAPSAPFHFTPPFRNPPDPTCGMKRLSRREAFY"
     gene            18202..18519
                     /locus_tag="ACFI8B_001368"
     CDS             18202..18519
                     /locus_tag="ACFI8B_001368"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3834575.1"
                     /translation="MASTGVNKEIKGKKLSLWTKRQDGSVKWFCGQPVKRESNNADAV
                     TADGTDGANGTKIETKHLPSTCRDESSAGCIETPRADFKHFQKISRYRILPESRQMAG
                     KQR"
     gene            18485..>19145
                     /locus_tag="ACFI8B_001369"
     CDS             18485..>19145
                     /locus_tag="ACFI8B_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3834576.1"
                     /translation="MNHGKWPENNGDAGVASSSSIIGKYVEEVKVENGVVTAKMKSDG
                     VNKEIQGKKLSLWAKRQAGSVKWFCGRPVTRANDAAKDAVTADAKDAIETKHLPSTCR
                     DKSSAGCTKTPEYYLNHGKWPEDNDKAGVASPADIKGKYVEEVKVENGVVTAKMKSDG
                     VNKEIQGKKLSLWAKRQAGSVKWFCGQPVKRTANNNDTVAADKDKEIDTKHLPSTCRD
                     KS"
BASE COUNT         4627 a         5779 c         4857 g         3882 t
ORIGIN      
        1 gccccaagaa atatgcagta gcattaggat ctagttctac tgccagtgga gagtattcct
       61 atgcgtcggg ctataattct gttgccagtg gaaataaatc ctatgctgca ggctatgctt
      121 ctgttgccag tgcagaaggc agtgttgtaa ttggcgattc aaggcaagta aaaccggaag
      181 cagatcaagg ggttgcagta ggttctaaag caacggtaaa gaacaaagct aaacaaaggg
      241 ttgcggtagg ttctgaggca aaggtaaatg cagaacgtgg tattgcgatt ggtaaagaag
      301 ccaaagcagg gggcaaaaca acgaacacac tgttagatgg accggcatat tatgcggacg
      361 ctattgccgt cgggtatcaa gcagaggctg gcaaaggtgg cgccatcgct ttgggaaaac
      421 aagcaaaagc aacgaagcaa aatggaatgg cacttggcgt agaatcagaa gctgccggtg
      481 attttagtac cgccgtgggt aatgaaagca aggcaaaggg gcaaggcggc gtaggtctcg
      541 gtaaccaatc taaggcagag gcagattttg cggtggctgt ggggaataag gctgaggcga
      601 ctaaggaaaa ctcacttgtc attggccgat atgcaagggc aaacggaaat cattctgtga
      661 gtttaggatc tcgtagtgaa ataaaagatg gtgtgagcaa ttctgtggca ccgggttatg
      721 gttcagtagc tagtgagaat aatgtcgtct ctgttgccta taaagaaaca ccacaatcta
      781 cagaactttc ctatagaaaa attgtaggtg ttgatgatgg tgtcaatgat tttgatgccg
      841 tcaatgtacg ccagtcgaaa gcgatgcaag gacaaaatat ggctgagcta ttttcagtac
      901 gcagcgaagt gcgtggtgtg gcagcgagta gtgcagccct atcggctctg actccattat
      961 cctatgatgc aaacaatcct acgcagttta tggtcggttt cggcacatac aaaggacgtc
     1021 aagctatggc attaggattg tcccatttcg tcaatgaccg ctttatggta aaagtaggcg
     1081 gaaccttagg cggcaataag acgggacata tgatgaatgt aggcttgact tggaaatttg
     1141 gaacgagcca aggtgttccg gctacaagtg ataaggtgct tatggaacag ttgatgcaag
     1201 ataatcgtca attaaagcca cgattggaga aattagaagc acgtctgaat gctatgtagc
     1261 cttgtggcga taacggttta ttgagtatat cgccaatgat aaagtgcata attttcaaac
     1321 cgttgataat atacaataaa ttgtaaccaa tacctcaaac gggtttgaac ccgaatatgc
     1381 ctgacaggaa ctttctgtca ggcattgttg ttgtgatgtt ctgcaatcat tttttggtac
     1441 acggccggcg gcacatatct tttgacggtt tccatccagc cctcgggccc gaccagtcct
     1501 ttgatcatag tggacgacac ttcggcgatt tcgcgcggcg gcatgaggaa tacggtagat
     1561 atttcgggtg cgaggtcgct gttgatatgg cgcatggagc gttcgtattc ataatcggag
     1621 gtggaacgga tgccgcgcac gatgaatccc gcatctacct cacgggcgta atgcaccaaa
     1681 aaccggtttt gaaacacttc aattctgacg tttgggaagt tgtcggtaat ggcgcacaac
     1741 atatcctgcc tttcagcggc ggtataggtg ttgcgtttgt cgggattgat gccgatggcg
     1801 acgatgagtt cgtcaaacat agattgcgcc tgccgtatca tccacagatg ccccaatgtg
     1861 ggcggatcga aactgccggc ataaacggcg cggcgcggtg tattcggtaa catttgattc
     1921 ctcccggctt catagccggc tgtgttttta tgtgtttgca tccgtattgt atgcccaaaa
     1981 caaaatgccg tctgaaaata agtgctttca gacggcatgg tttgcatggt gtttcgccta
     2041 catcagtcgt cgccgtttaa ggacagcagg atgttgagca ggctgctgaa gatgttgtaa
     2101 agcgagataa acagtgtcag tgccgcgctg atgtaactgt cttcgccgcc gtcgatgacg
     2161 gtgcgtacct gccacataat cattaaggaa ctgaacaaga caaaaccggc ggaaatggtc
     2221 agggcgagtg cgggaatacc caaaaacaga ttggcaacca cggcgaccat cagaatgacc
     2281 gcacctacgg tcaggaagcg tccgagcgcg ttcatatcga gccgggttcg gcgtgccaat
     2341 gcggacatcg ttaaaaagac ggcggcggtc atcgcggcgg caatgccgac gattttcgca
     2401 ccgtcggcaa tatggagtgc gtattgcagc acggggccga tcaatacgcc cataccgaat
     2461 gtgaatacca tcagcagggt aacgccggta ttgctgtaac ggtttttttc gatgaagtgg
     2521 atcataccgt agaaaaacgc caatacgacg gcaaatccta tccagcgcga accgaaggcg
     2581 gcgtaaaaat tgaaaccggc attggcggca agtgccgcgc ctgcggctgc cggaatgaat
     2641 gaaaatccga gcaggcggta ggttttctgc aggacggtgt ttttagaaac cgtatgcgcg
     2701 gtgtagtcgt aaacgtcgtg ttgcatatca tctgctcctg aaagcgcggt tggaaataac
     2761 gggggatttt aacattgccc aatgtcaaaa tttgtccggt tgcgtgaaga taaagttgtc
     2821 cggcgtattt taaaggccgt ctgaagcagt ttcggacagc ctgtgttcaa aacggaaaac
     2881 cgttattgcg gaacgtatcc ctgcacggca tccgcgccgt cgccgaagaa atactgctcc
     2941 atctgctgag ccaggtattc gcgcgcgcgc ggatcggcaa ggcttaagcg gttttcgtta
     3001 atcagcatgg tttggtggcg cgtccacgcc gcccatgctt cttgcgatac gttttcaaaa
     3061 atgcgtttgc ccaattcgtt gggcagcggc ggaaatttca tgccttcggc ttctttgttg
     3121 agcttgacgc agaataccat tcgtgccata gcggattcct ttgctgtgtt cagaaataac
     3181 ggggcgattt taaccgatta gggggtacag acaaaagcct ttttattccc gatgataagg
     3241 atggttgtgc aggatggaaa cggcccggta gagctgctcg gtcagaagga cgcgcaccat
     3301 gccgtgcggc agggtgaggc tggacaggcg catcatcatg cgggcttgtt gtttgaggcg
     3361 gtcggtcatg ccgtccgcgc cgccgatgac gaagcagacg tgttcgccgt tttgccgcca
     3421 gcttttgagg tgttccgcca gctcgacgga ggtcggtgct ttgccgcgtt cgtcaagaac
     3481 gacgaggaac gcgccttgcg gaatggcttc aaggatgcgt ttttcttccg ccgccatacc
     3541 ttggacggca ttcacgcccg cgccgcgttt ttcgggtttg atttctttga atgcgtaggc
     3601 ggcgtcgcgt ccgaagcgtt tggcgtattc ggcgacggcc tcatcaaccc aacgcggcat
     3661 tttggtgccg actgccaaga cggtaatgtt caatgctttc ccccttacag gaaatgccgt
     3721 atgaatattc agacggtatc gggaatcagt ctgcagcgtg ccacggcttc tgcattccgg
     3781 cgtggaaact cggtttctcg ccgccccaga tggtgtcaat gtcgtagaag tcgcgcacgg
     3841 cagggagcat gacgtggacg acgaggtctc ctgcatcaac caacgtccat tcgccgctgt
     3901 ctccttcggt actgaggatt tcaaaaccgg cttctttcaa atcgacggca acgttgttgg
     3961 ccagtgcttt gacttggcgc gtactgtcgc cgctggcgat aatcattctg gcaaacagcg
     4021 aagttttgtc ttgcgtttcg agaacggaaa tgtctttggc tttgatgtct tcgagggcat
     4081 tgacggcgac cccgaccatt ttttgcaggt cttgcagttc ttgttcgttc attgttttcc
     4141 taacgggatg ttttcagacg gcattatagc ctctccttac tgatttgact ttatttttca
     4201 tacaaaccgt gttttcggat ataacgggcg gcggcaggcg ggatgccgtc tgaaacaccg
     4261 gcggcactca ggttgcggcg gatttccgtt gacgacacat tatgcatcgg ggctgacaag
     4321 atgcggacgc tgccgtcctg aagggcattg cccagccacg cgtgcagttc gcgcggggtt
     4381 tggtgcaggc tgtcgccctg ccgcatggcg acggcgatat tggtttcgcg cacgagcaac
     4441 tgccattttt tccaagtatg cagcttcagc aggctgtcgc tgcccatcag ccaccaaagt
     4501 tgcgcggacg ggaactgccg gcggaagatt tggacggtat caaaagtata ggttgcactt
     4561 tctcggacga tgtcgcagtc gctgacggca aaacgcgcgt cttctgccgt cgccagttcg
     4621 accatggcaa ggcggtcggc ggcggaagcg gaggcggcgt ctttgtgata cgggccgcct
     4681 gccggcagga aaacaaccgc gtccaacccg atttcgtcgg caaaggcacg ggcgatatga
     4741 aaatgcccgt tgtgtatcgg gtcgaaagtg ccgccgaaca gtccgatttt cttcatgatg
     4801 tttccattcc ttccgataag tccatgccgt ctgaaacact gccgtccaca accagcgtca
     4861 gtttgccggt aacgggatgg atgaccagtc cgtgaacggc gatatggcgc ggcatcagcg
     4921 gatggttgcg gataaggtcc accgtgtggc gcacgctgtc ttcgacgttg tcgaaaccgg
     4981 tcaggtcgag gtcgacacct gcataacgca gggtttcgat acggtcttcg ggaatccggc
     5041 tttcccggac gcgcccgagg aattcttcgg cattcagccc ctgcataccg caatcgtgat
     5101 gggcgatgac cataatctct ctgaccttca attcaaacac ggcaaccaaa aggctccgca
     5161 tcaccgaacc ccacgggtgc gtaaccagcg cgccggcatt tttaatcagc ttggcatcgc
     5221 cgtttttcaa acccaacgcg tcgggcagca gcccgataat ccgcgcatcc atacaggaca
     5281 aaaccgccag cccgcgttcg gggtatttgt cggtaaagta tttttcatat cctcccgact
     5341 cgacaaactt ctgattatag gaaaggattt cgctcaactc gctcattttg ccgtcctctg
     5401 aaaaagggtt cgcattataa cgtttccgtc cgttttccgc cttcgccgtt gtccaacagc
     5461 aggaaaatgc acaccgcaaa tgccgacaac agcaatgtca acaccattgc ccgcgcataa
     5521 ttgtcctcac ccgcacgccc caaataggca taaatcaaag tcgtcaacgt ctgccattcc
     5581 ggacgcgaca ggaacaaggt tgccgcaaat tcgcccacac acgtcgccgc cgccaaagtc
     5641 agaccgcgcc gcaacgccgg tttcaagagg gggaacgtga tacggcatgc cgtctgaaag
     5701 ccgtttgcgc ccaaacctgc cgccgccctg ccgtaatccg gcggcagtgc atcccaggcc
     5761 gataaaacat cttttgccac aaacggatac gccagcagcg catacatcgc cagcagcagc
     5821 ggtaacgaag ccgtccaccc cggataaagc agcagcacgc ccgccgaaac acaaaccggc
     5881 gacaccataa acggtaaaaa caccagtccg cgcatccacg ccagccgccg cgccgccgcc
     5941 gcatacacca cacccaaaac cgccgccgca aacaccgccg ccgccgaaaa gcgcaaagta
     6001 ttccacactg cctgccacgt ttcactttcc attaacacac gccgcgattc gccggccgac
     6061 cacgctttca caacaattgc cgacaaagga aacaggcagc acacggacaa caccgccacc
     6121 gaaaatgcca gcaatacata ttcccccacc gattgcggcg gcgacggcat cacgggggaa
     6181 accgccttat ccgaaaccgc gcgcctgccg aaccacgcat acagcaaccc tgccgccgcc
     6241 gttaccccca acaccagcca caccagcgcc gaagcccccg ccatatcgag ttcgaacata
     6301 accaactggt aaatttccac ttcgaccgtg gcataacggc tgccgcccaa cagcaatgcc
     6361 agcccgaacc ccgaaaaaca atacaggaag acaaggcaca cgccgccggc aagccacggg
     6421 cgcaaaacgg gcatttcaat gtcccaaaac cgccgccacg cccccgcgcc caacgtccgt
     6481 gccgtctgaa gccgtgccgc aggcacttga gcaaacccct gatacgccgc cctgaccaac
     6541 acgggcaggt tgaaaaacac attgccgtac aacaacagat acggcgtatc ctgccggccg
     6601 cgccacaaca gcccgtccgc cccgaacaga gccagcacgc ccacgcccgc caccagcgtc
     6661 ggcatcacaa acggcagcat cagcaggcgc agcaccaaag cccgccccgg gaacgccagc
     6721 cgcgccagca cccacgcgac aggcacgccc aaaggcagca ccagcacaca ggttgccgcc
     6781 gcctgaaaca ccgtccacgc caaacgtttg agcatatagg catccgacag caccgcgcgc
     6841 cacgccaaac cgtcatacgc cgccaccgcc cacaaaggcg caacgaccat taccgccaaa
     6901 aaagccgaag gcagcaggga aaaagcaccc cgtaccgccc aacgccgtcc atccatcgcc
     6961 ttccccactt gaaacactga tgttgcgatt gtacccaaaa gccccacata ccgtatattt
     7021 caatccgact acgtaccgta tccgccttcc tcccgccgtc tgaaatatag cggattaaca
     7081 aaaatcagga caaggcggcg gaccgcaggc agtacgaatg gtacggaacc ggttcgcccg
     7141 gtgcttcagc accttaggga accgttccct ttgagctaag gcggggcaac gccgtaccgg
     7201 tttttgttaa tccactataa atcgttcaaa taaacaggaa tataacttca gacaaacaac
     7261 ttaccgcccg atttgtgcta tcgttttcgc acaacttaaa aaaacctgac aattttgtac
     7321 ttttattaca gagaaaggct ttacaaatgg acggccggac acagacgctg tccgcgcaaa
     7381 ccctgttggg catttcggcg gcggcaatca tcctcattct gattttaatc gtcaaattcc
     7441 gcatccgcgc gctgctgaca ctggtcatcg ccagcctgct gacggctttg gcaaccggtt
     7501 tgcccacagg cagcatcgtc aacgacgtac tggtcaaaaa cttcggcggc acgctcggcg
     7561 gcgtggcgct tctggtcggt ctgggcgcaa tgctcggacg tttggtagaa acatccggcg
     7621 gcgcacagtc gctggcggac gcgctgatcc ggatgttcgg cgaaaaacgc gcaccgttcg
     7681 cgccgggcgt tgcctcgctg attttcggct tcccgatttt cttcgatgcc ggactgatcg
     7741 tcatgctgcc catcgtattc gccaccgcac ggcgcatgaa acaggacgta ctgcccttcg
     7801 cgcttgcctc cgtcggcgca ttttccgtca tgcacgtctt cctgccgccc catccgggcc
     7861 cgattgccgc ttccgaattt tacggcgcga acatcggcca ggttttgatt ttgggtctgc
     7921 cgaccgcctt catcacatgg tatttcagcg gctatatgct cggcaaagtg ttggggcgcg
     7981 ccatccatgt tcccgttccc gaactgctca gcggcggcac gcgagacagc gacccgccga
     8041 aagaacctgc caaagcagga acggtcgtcg ccgtcatgct gattcccatg ctgctgattt
     8101 tcctgaatac cggcgtatcg gccctcatca gcgaaaaact cgtgagcgcg gacgaaacct
     8161 gggttcagac ggcaaaaatg atcggttcga cacctgtcgc ccttctgatt tccgtattgg
     8221 ccgcactgtt ggtcttggga cgcaaacgcg gcgaaagcgg cagcacgttg gaaaaaaccg
     8281 tggacggcgc actcgccccc gtctgtcccg tgattctgat taccggcgcg ggcggtatgt
     8341 tcggcggcgt tttgcgcgct tccggcatcg gcaaggcact cgccgacagc atggcggatt
     8401 tgggcattcc cgtccttttg ggctgcttcc ttgccgcctt ggcactgcgt atcgcgcaag
     8461 gttcggcaac cgtcgccctg accacagccg ccgcgctgat ggctcctgcc gttgccgccg
     8521 ccggctttac cgactggcag ctcgcctgta tcgtattggc aacggcggcg ggttcggtcg
     8581 gttgcagcca cttcaacgac tccggcttct ggctggtcgg ccgcctcttg gatatggacg
     8641 taccgaccac gctgaaaacc tggacggtca accaaaccct catcgcattc atcggctttg
     8701 ccttgtccgc actgctgttt gccatcgtct gacagacgga aaggatagta aatgactacg
     8761 cattttgtcg ttatgggcgt atgcggctgc ggcaagacca ccgccgcgcc gtccctgcag
     8821 aaacacctcg gtcaatgtcc ctatgccgaa ggcgacgagt tccacaccca agccaaccgc
     8881 gacaagatgg gcgcgggtat tccgctgacc gatgaagacc gctatccgtg gttgggcaat
     8941 ctgcgcgact ggatgacgca acaggcgcaa aacggcgcgg accacaccat cgtaacctgt
     9001 tccgccctca aacgcggcta ccgcgacatt ctgcgcggag ccgaaggcaa agccgccttc
     9061 atccacctca gtccgccgca agacatcaac ctcgaacgca tgatgtcgcg caaagggcac
     9121 tacatgaaag cagggatgct cgattcgcaa ctggaaatcc tcgaggaact gggcgaaggc
     9181 gaatacgggg tcaaaatcgc caaccccggc acgcccgaag cggtcgaagc cgatattctg
     9241 aactgggttg cctcggaaaa cctgctttga agcaatatgc cgtctgaagc ccggcacagg
     9301 atgggtttca gacggcataa acatcgggaa cagaatgaat tacattgatt taaaatggaa
     9361 aatacccggc tatcgtctca ttttcgtttt aatcagccat aaaaatgccg tccgaaaccc
     9421 tttcagacgg catttctgtc aaacgccgga cgcactcaat ccaaactcaa cagcaggttg
     9481 cggaacgcgt tcgggtcttt gataaacgtc atctcgcccg cctgcggaaa atgaaaatcc
     9541 aacaggcgcg acacccaaaa acggatgcag ccggcgcgct gggcggtcgg gaaatacgcc
     9601 ttttccccgg cactcaaggg gcgcacgccc tcataaccgc cgataaacgc ctttttcaac
     9661 gcctcatcca acttattgtc cgccgtcctt gcccaatcgt tgaccgcaat cgccaagtca
     9721 tacataaaat tgccccggca ggcataatag aaatcgatga agcccgatac ctgaccgccg
     9781 tcaagcaaca cattgtcttt aaacaggtcg gcgtggatga tgcccgaagg cagatggttg
     9841 ccgagattgt ccttcagcgc atcgatttcg gaacacagca gcgcggcatc gtcttgcgac
     9901 aggacgggca gcagccgggc gcacgcttcc gtccaccacg catcgtaacg cgggttttcc
     9961 atttccaaag ggaaatcggc ggcggcaagg tgcattttcg ccaacatcgc accggtatgg
    10021 aaacactgct cagccgtcgg cagcgcggta tccgaacctt tcaggcaggc aaccaggcag
    10081 gccggcttgc ccgccaaaac ggaatcgagc cggccgtctt tgcgcgcaac cggcgcggca
    10141 accgccacgc ccttcatact caaatgccgg ttaagctcca gaaaaaacgg cagctcttcc
    10201 tgtttcaaca cttcaaacac ggtcagcaca taacgtcccg aagtcgtcgt cagaaaataa
    10261 ttgctgttgg taatcccctg cgcaatgccc tgcagggaaa caaattcccc caaatcgtaa
    10321 ccgctcagga agccgcgcat ttcatcatcg gaaacactgg tatagacaga catatcaact
    10381 cacttgctca aaaacgggca aacccgccgt cagaacgccg gacggcaggc aaacatataa
    10441 ttcacatcgg tcgaatcgca cagggcataa gtttgcgaca acacatggta agtcataccc
    10501 ttcgtatccg ccacatccaa gcccgcctga cggcacatcc gcgccagctc ggcaggcgag
    10561 atgaattttt tccagtcgtg cgtacctttg gggacaaact tcaacagata ttccgccgcc
    10621 acaatcagat gcaggtacga tttcgggttt ttattgatgg tggaaaaaaa caccatgccg
    10681 tccggcctga ccagcttggc acaggcacgc acgatggcgg cgggatcggg gacgtgttcc
    10741 atcatttcca tgcacgttac cacatcgaac gagtgcggtt ccgccccggc aaggtcttcc
    10801 acgcggacac attcgtattc gatatcggcg acattgttca aagccgcgtg caggcgggcg
    10861 gtttccaacg actgctccgc catgtcgata cctttgacaa acgccgcgcc gcgccgcgcc
    10921 atgctttccg cgaggatgcc gccgccacag cctacatcta aaacccgttt gccgcgcaaa
    10981 tccgcgtgtc cgtcgatata atccagccgc agcggattga tgtcgtgcaa ggttttgaac
    11041 tcgcccgact tgtcccacca tttgtcggca atccggctga acttggcgat ttccccctca
    11101 tcgacattat attttttgtc ggacattttc cctcccatct gacgaaccgc cccactcaaa
    11161 aacccaagat tatactatgg gacgcaatgc cgtctgaaag ccttttcggg cggcgcggcg
    11221 caaatacctt acaaaccctt acactttacg gcataatgca ggctgttttt cccgaggagg
    11281 cttatatgtc cgccaaaact ccgtcgctct tcggcggcgc gatgattatc gccggtacgg
    11341 tcatcggcgc aggtatgctc gccaacccca ccgccacctc cggcgtatgg tttaccggct
    11401 cgctggccgt gttgctgtac acctggtttt ccatgctctc cggcggcctg atgattttgg
    11461 aagtcaacac ccattatccg cacggcgcaa gtttcgacac gatggtcaaa gacctgctcg
    11521 gacgcggctg gaacatcatc aacggcatcg ccgtcgcctt cgttttatac ctgctcacct
    11581 acgcctacat cttcgtcggc ggcgacctga ccgccaaagg catcggcagc gcagtaggcg
    11641 gcaaaatttc gctcaccgtc ggacaactcg tcttcttcgg catcctcgcc ttttgcgtat
    11701 gggcatccgc acgcttggtc gaccgcttta ccggcgtcct catcggcggc atggtattaa
    11761 cctttatttg ggcaaccggc ggcctggttg ccgatgccaa accgtccgtc ctcttcgaca
    11821 cccaagcccc cgccggcacc ggctactgga tttacgccgc caccgccctg cccgtctgcc
    11881 tcgcttcctt cggcttccac ggcaacgttt ccagcctgct caaatacttt aaaggcgacg
    11941 cgcccaaagt ggcgaaatcc atctgggcag gtacgttggt tgccttggta atttacgtcc
    12001 tctggcaaac cgccatccaa ggcaacctgc cgcgcaacga gttcgccccc gtgattgccg
    12061 ccgaagggca agtctccgtc ctgattgaaa ccctgtccaa attcgcccaa accggtaata
    12121 tggataaaat attgtcccta ttttcctaca tggcaatcgc cacctcgttt ttaggcgtaa
    12181 ccttaggcct gttcgactac atcgccgaca tcttcaaatg gaacgacagc atgtccggcc
    12241 gcaccaaaac cgccgcgctg accttcctgc cgcccctgat ttcctgcctg ctcttcccca
    12301 ccggcttcgt taccgccatc ggctgcgtcg gcctggcggc aaccgtctgg acaggcatca
    12361 tccccgccat gctgctctac cgttcgcgca aaaaattcgg cgcaggcaaa acctataaag
    12421 tttacggcgg cttgtggctg atggtttggg tcttcctttt cggcatcgcc aacatcgccg
    12481 cacaggtatt gagccaaatg gaactcgtcc ccgtatttaa aggataaagg caaaatgccg
    12541 tttgaaaccc gccggcggct tcaggcggca ttgccgcaac aaacggcaac cgtattccgg
    12601 cacacagcgc attaccctac cccctcacgc acaaatcccg ccccgacagg cgcgggacgc
    12661 aaccataaag gaacaatgat gaagctcaaa atagacattg caaccaacaa cttcaaacac
    12721 ggcggcggca cggaacgcta cacattggat ttggtaaagg gtctgaacag acaaaacatc
    12781 acaccggccg tttatgcgac gaaatttgat cacggcattc ccgaatacgc catgatcgaa
    12841 ccccatcttg tcgatcaacg ccggacgctg aaaaaactac gctcgttcct cttttcaagc
    12901 cggctcgctc aaaccagaaa aaacagtgcc gccaaactga ttgcctgcca ccacgccgat
    12961 tacgccgacc tcctcatctg cggcggcacg cacttgggct acctgcacca tatggcgcaa
    13021 aaaccgaacc tgctcgaccg cctcgccata cgccgcaacc gcagcaacta cgccaccgcc
    13081 aaactgattg tggcgcattc ccatatgatg cggcgcgaac tggtcggact atacggcgtt
    13141 ccccccgaaa gaatccaagt cgcccccccc cccgcagata cggaacgctt ctttccacaa
    13201 cctagagaaa ctgccgccct gcgcgccaaa tacggttttg ccgaccatga aaccgttttc
    13261 ctattcccat cgaccggcca cacgcgcaaa ggcctggaac tgctcgccga ctttttcgaa
    13321 caaaccgaac tgcccgtcaa gctcgccgtt gccggttcac cgcttccccg cccgatgaaa
    13381 aacgtcgtcg gactgggctt ctgcaccgat atgcccgaac tctaccgcgc cgccgacttt
    13441 accattatgg catccctgta cgaacccttc gggctggtcg gtgtcgaatc cgccctatgc
    13501 ggcacacgcg tcgtcctctc cgaaaacatg gcatgtacag aggtcatgaa cgaagaagcc
    13561 ggattctttt tctcacgaca aaacccggaa accctcgcgc aagccgttgc ccgggccgtc
    13621 agccttaaaa aacagggcgg acaccgcctg tccgacccga tgcgggcgct gaactacaac
    13681 ccgtccctgt cgcaccacat cgaccgactg accgacatgc tggcatccgt ataacaaaat
    13741 gccgtctgaa cgggttttca gacggcattt tcgcaaccgt tgcagccgtg cggcaaaaag
    13801 ctaacgggca attttcaaaa cgtcgtacag atagttgcgg taatttccga ccaactcccg
    13861 gttcgggcag tcttccaacc ctccgccgca caaaccgcgc atcgccatat tcgcacgcat
    13921 gaagccgtct ttggcttcca aaccagccgc ctccatcagt acgccgcctg caaaggcaag
    13981 gtcgaggaaa tcctgccgct gtacgaatcc gccggcaata ttgctcctga cggcaaactg
    14041 cgttacataa tccggctgag cgtaatccca tttcttcctg acggacacgc cctcaaacgg
    14101 tacctgatga tcgccgaaat aaccgaaaac aaagggtttg ccgcgttcgt gcagataacg
    14161 gtcgaaactt tccaccgctt tgtccaaatc ggcaatgcgc ccgatgtagt cgttaagcgc
    14221 ggatacggtt ttcgcattca aatcgggcgc atccaaatca aacacattgt cggtatccgt
    14281 ccgatacggc ccgtgctcct tcatggtcag cacatatacg aacatcggct gccgcacgtt
    14341 ttccaaatcg ggatggcgtt tttcgagaat catccgcgca tactgcatca tttcctcact
    14401 ggaaatgtgc cacaggtttt tgcccatcgg cgcgggatag cccaaatctt gcggctggaa
    14461 catcagatta aagccgaaat ggtcatatgc tgccttggca ttgtagttgc ccttggtaaa
    14521 cggcgagagc gccacgcaaa aataaccgtg ttcgcgcagg ttgcggacaa aaccggtctg
    14581 caaatgcggt acgaccgaat aaaacacgcc gcttgccaac gcgccgaaat ctgtggacgg
    14641 aacacccgcc aaaaacgcaa attcggactt ccacgttgcg ccgccgaaag tatgcacgcg
    14701 caaaggtgag gaaaatacag tatcttcctg ccgtccgaac attttcaaat cgggaatttt
    14761 ggcggcggca aaatcgaaac agtgcggatc gagcgtcgat tccatcaggg taacgacaat
    14821 atcgggcttt tcatccgaca tgccgtacgg ctgggtttca gacggcatct gcctggcaaa
    14881 cgcttccccg tcgccctcga acacgggtac ttggaaaaac accgcccgac aggacatcgg
    14941 caggttcaaa aacacgtcgc gcccgtcatc cggcagcgaa tccagccacg tctttaccgc
    15001 gccggggtgc ttggaaaaat gccgcaccga cacgaacgcc gccgcaaaca gaaccgcgcc
    15061 cgcccaacgc cacggcatac ccaaagaatc cgcaccgctc cagccgaaaa ccgcatatgc
    15121 cgccaaagcc agcagccccg ccataccgat aaccgcttcc ttataatgaa acagcgtttc
    15181 ccaattccgc cagtcggcaa tcagcaaaaa gtcggaaatc aacagcggct gtttgtaata
    15241 atggattttc agcctgtgga acagtatcag caccacaaac cagaccgaag cgaaattcaa
    15301 ggcgcgctgc cactgcgccg tcaacatcag cataccgccc gccaaaaaga caaacagcgc
    15361 cgacgcgaaa aaatacgtcc agcggtagtg cgacctgaca atcagcagca ccaccgccgt
    15421 tacaaacaaa aataggaaag cataggcaac catcccgccc cactcctcaa aacggcatac
    15481 ctgtgccgtt gcgaattatt atcgaaagca tgcattataa tgcagcacag gcgcatttcc
    15541 accgatattt cagtataatg ccatccccga acccgcccaa cccaaaggaa ccgtgatgaa
    15601 actcatcatt ctcgaccgcg acggcgtcat caaccgggac cgcgacgact tcgtcaaatc
    15661 cgccgacgag tggatacctg tcgaaggcag catggatgcg gtggcattcc tgacgcaggc
    15721 aggctgcacc gttgccgtcg ccaccaacca atccggcatc ggccgcaaat attttaccgt
    15781 tcaagacctc accgaaatgc atgccaaaat gcaccgcctc atccgtcagg cgggcggcga
    15841 aatcgacggc atctggttct gcccgcatac cggcgccgac ggctgtaact gccgcaagcc
    15901 caaaccgggc atgattgaag acatcatcgg acgcttcaac gcccaagctt cggaaacttg
    15961 gctggtcggc gacagcctgc gcgatttgca ggcaatcgat gccgtcggcg gaaaacccgc
    16021 gctggttctg accggaaaag gcaaaaaaac gctctcccaa cacggacacg aattacccgg
    16081 acacacacag gttttcgata ccctgctcga tttctcacaa tacatcatgc aggaaaacgc
    16141 cgcaccgcaa gccgactgaa cataccgcat ttccgacaag gcaaaaccat gctcatcatc
    16201 cgcaacctga tttactggct gatactctgt tccagcctga ttttcctctt tccctttatg
    16261 ctgctcgcct cgcctttccg ggacggggcg cacaagatgg cgcgggtctg ggtcggcatc
    16321 ctcaactggt cgctcaaaca catcgtcggg ctcaaatacc gcatcatcgg cgcggaacac
    16381 attccggacc gcccctccgt catctgcgcc aaacaccaaa gcggctggga aacgctcgcg
    16441 ctccaagaga tttttccgcc gcaggtttac gttgccaagc gcgagttgtt caaaatcccc
    16501 tttttcggct ggggcttgaa actggtcaaa accataggca tagaccgcaa caaccgccgc
    16561 gaagccaacg aacagctcat aaaacagggt ttggcgcgca aaaacgaagg ttattggatt
    16621 accattttcc ccgaaggcac gcgccttgcg cccggaaaac gcggcaaata caaactcggc
    16681 ggcgcgcgca tggcgaaaat gtttgagatg gacatcgtcc ccgtcgccct caacagcggc
    16741 gaattttggc cgaaaaattc ctttctgaaa tatccggggg aaattaccgt catcatctgt
    16801 ccgaccatcc cgcacgcaag cggcagcgaa gccgaattga tggaaaaatg cgaacacctc
    16861 attgaaacgc aacaaccgct tatttccggc gcaggcccgt ttgccgccga aatgccgtct
    16921 gaaaccgcat gacctccctg attcacactc tttcagacgg catagaactg accgtcgaaa
    16981 tcaaccgccg tgccaagaaa aacctgatta tccgccctat cggcacacat accgtccgca
    17041 tcagcgtccc accctgcttc tccgtctccg ctctaaaccg ctggctgtat gaaaacgaag
    17101 ccgtcctgcg gcggacactg gcgaaaacac cgccgcacaa tacggcaaac cggctgcccg
    17161 aacatatctg gttccacggc gggcggcttg ccccaccgcc catcaggaca cgcaaatcct
    17221 gctgatgccg cctgaaatcc gcgtccccga aggcgcgccc gaaaaacagc ttgcgctgct
    17281 gcgggacttt ttggaacggc aggcgcacag ttacctgatt ccccgtctcg aacgccacgc
    17341 ccgcgccacg caactgttcc ccgcctcctc ctcgctgacc tccgccaaaa ccttctgggg
    17401 cgtgtgccgc aaaaccacag gcatacgcct caaccggcgg ctggtcggcg cacccgaata
    17461 cgttgccgac tatgtctgca tacacgaact ctgccacctc gcccatcccg accacagccc
    17521 cgccttttgg gaactgaccc gccgcttcgc cccctacacg cccgaagcaa aacagcggat
    17581 caaaatccac ggcagggagc ttttcgcctt gggctgacgc agccccgggc cgacgctttc
    17641 agacggcatc cgtgccggaa caggcacgcg cccgcccgat tcaaaccgcg atgacgcttt
    17701 gccgccggtt cggggcagga tggcggcatc cgcgccgtcc gcaccgtttc atttcacacc
    17761 gcccttccga aacccgcccg atccgacgtg cggtatgaaa cgcttaagcc ggcgcgaagc
    17821 cttctactga tttgcccgcg aaaatgccgt ccgaaaggtt ttcggacggc atttttttgc
    17881 gttttcctgg atggcggcgg attcgtaaaa ggcgggcagg gtggattgca ggatgggttg
    17941 aggcccgcca aatccgccaa ctctaccgaa tacgccgccg tcattcccgc gcaggcggga
    18001 atttggtttt gtcggtgcgg aaacttatcg gataaaacgg tttcttagat tccacgtcct
    18061 agattcccgc ctgcgcggga atgacggttc gggtatttct gacgattcat cgagccgtag
    18121 caactgtatt ttccaccccg tcgggcaaaa ataccaaaac tcaaatcaag ccgtccgaat
    18181 accgttttcg gcggtatcgt tatggcttca accggcgtaa acaaagaaat caaaggcaaa
    18241 aaactctccc tgtggaccaa gcgtcaagac ggttcggtaa aatggttctg cggacagccg
    18301 gttaagcgcg aaagcaacaa cgccgacgcc gtcaccgccg acggcaccga cggcgccaac
    18361 ggcaccaaaa tcgaaaccaa gcacctgccg tcaacctgcc gcgatgaatc atctgccggt
    18421 tgcatagaaa caccacgcgc cgatttcaaa cacttccaaa aaatcagccg ttaccgaata
    18481 ttacctgaat cacggcaaat ggccggaaaa caacggtgat gccggcgtgg catcttcttc
    18541 atcaatcata ggcaaatatg ttgaggaagt taaagtcgaa aacggcgtcg ttaccgccaa
    18601 aatgaaatca gacggcgtaa acaaagaaat ccaaggcaaa aaactctccc tgtgggccaa
    18661 gcgtcaagcc ggttcggtaa aatggttctg cggacggccg gttacgcgcg ccaacgacgc
    18721 cgccaaagac gccgttaccg ccgacgccaa agacgccatc gaaaccaagc acctgccgtc
    18781 aacctgccgc gataaatcat ctgccggttg cacaaaaaca cccgaatatt acctgaatca
    18841 cggcaaatgg ccggaagaca acgacaaggc cggcgtggca tcccccgccg acatcaaagg
    18901 caaatatgtt gaggaagtta aagtcgaaaa cggcgtcgtt accgccaaaa tgaaatcaga
    18961 cggcgtaaac aaagaaatcc aaggcaaaaa actctccctg tgggccaagc gtcaagccgg
    19021 ttcggtaaaa tggttctgcg gacagccggt taagcgcacc gccaacaaca acgacaccgt
    19081 tgccgccgac aaagacaaag aaatcgacac caagcacctg ccgtcaacct gccgcgataa
    19141 atcaa
//
LOCUS       DAXFWM010000043        19114 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000043 DAXFWM010000000
VERSION     DAXFWM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19114
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.043"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..779
                     /gene="pglE"
                     /locus_tag="ACFI8B_001370"
     CDS             <1..779
                     /gene="pglE"
                     /locus_tag="ACFI8B_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HFC3834577.1"
                     /translation="YQDNQGLSEARNTGIKNSNGKYIAFIDSDDFINCQVLLDFLGKD
                     DSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGLCRFRKFPGSACNKI
                     IKRELIIKEKLFFEKGIYAEDIEWSMRLFNAATTFSYLDGCYYYYRQGRKDSITGTVS
                     EKGIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACDSDIKRRAY
                     HLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            820..1350
                     /locus_tag="ACFI8B_001371"
     CDS             820..1350
                     /locus_tag="ACFI8B_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC3834578.1"
                     /translation="MNAIRTFQNRTPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            1461..1616
                     /locus_tag="ACFI8B_001372"
     CDS             1461..1616
                     /locus_tag="ACFI8B_001372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834579.1"
                     /translation="MPSESLFFGFGRHFIADFFLEFDRMFARFVEHDVRPTFLRQSQI
                     HALGRTR"
     gene            complement(1724..3319)
                     /locus_tag="ACFI8B_001373"
     CDS             complement(1724..3319)
                     /locus_tag="ACFI8B_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC3834580.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3426..3821)
                     /gene="hisI"
                     /locus_tag="ACFI8B_001374"
     CDS             complement(3426..3821)
                     /gene="hisI"
                     /locus_tag="ACFI8B_001374"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC3834581.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3852..4619)
                     /gene="hisF"
                     /locus_tag="ACFI8B_001375"
     CDS             complement(3852..4619)
                     /gene="hisF"
                     /locus_tag="ACFI8B_001375"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC3834582.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEITIREAKRTMREAGIEVRL"
     gene            complement(4632..5369)
                     /gene="hisA"
                     /locus_tag="ACFI8B_001376"
     CDS             complement(4632..5369)
                     /gene="hisA"
                     /locus_tag="ACFI8B_001376"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC3834583.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5402..6040)
                     /gene="hisH"
                     /locus_tag="ACFI8B_001377"
     CDS             complement(5402..6040)
                     /gene="hisH"
                     /locus_tag="ACFI8B_001377"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC3834584.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6170..7672)
                     /gene="pta"
                     /locus_tag="ACFI8B_001378"
     CDS             complement(6170..7672)
                     /gene="pta"
                     /locus_tag="ACFI8B_001378"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC3834585.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLTQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7857..8915)
                     /gene="fbpC"
                     /locus_tag="ACFI8B_001379"
     CDS             complement(7857..8915)
                     /gene="fbpC"
                     /locus_tag="ACFI8B_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC3834586.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8936..10453)
                     /gene="fbpB"
                     /locus_tag="ACFI8B_001380"
     CDS             complement(8936..10453)
                     /gene="fbpB"
                     /locus_tag="ACFI8B_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC3834587.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10521..11516)
                     /gene="fbpA"
                     /locus_tag="ACFI8B_001381"
     CDS             complement(10521..11516)
                     /gene="fbpA"
                     /locus_tag="ACFI8B_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC3834588.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11768..12248)
                     /locus_tag="ACFI8B_001382"
                     /pseudo
     CDS             complement(11768..12248)
                     /locus_tag="ACFI8B_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(12306..13682)
                     /gene="argH"
                     /locus_tag="ACFI8B_001383"
     CDS             complement(12306..13682)
                     /gene="argH"
                     /locus_tag="ACFI8B_001383"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC3834589.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHLLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(13701..14570)
                     /gene="galU"
                     /locus_tag="ACFI8B_001384"
     CDS             complement(13701..14570)
                     /gene="galU"
                     /locus_tag="ACFI8B_001384"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC3834590.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14598..15197)
                     /gene="rdgB"
                     /locus_tag="ACFI8B_001385"
     CDS             complement(14598..15197)
                     /gene="rdgB"
                     /locus_tag="ACFI8B_001385"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC3834591.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(15190..15423)
                     /locus_tag="ACFI8B_001386"
     CDS             complement(15190..15423)
                     /locus_tag="ACFI8B_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC3834592.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(15531..16064)
                     /locus_tag="ACFI8B_001387"
     CDS             complement(15531..16064)
                     /locus_tag="ACFI8B_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC3834593.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(16166..16624)
                     /gene="nudB"
                     /locus_tag="ACFI8B_001388"
     CDS             complement(16166..16624)
                     /gene="nudB"
                     /locus_tag="ACFI8B_001388"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC3834594.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16835..16911
                     /locus_tag="ACFI8B_001389"
     tRNA            16835..16911
                     /locus_tag="ACFI8B_001389"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16869..16871,aa:Pro,seq:ggg)
     gene            17174..19060
                     /gene="mafB"
                     /locus_tag="ACFI8B_001390"
     CDS             17174..19060
                     /gene="mafB"
                     /locus_tag="ACFI8B_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC3834595.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
BASE COUNT         4099 a         4576 c         5764 g         4675 t
ORIGIN      
        1 tatatcagga taaccaagga ttgtcggagg cgagaaatac cggaataaaa aattcaaatg
       61 gaaaatatat agcatttatt gattcggatg attttattaa ttgtcaggtt ttgctggatt
      121 ttcttggtaa agatgatagt gatatgccgg atgtggtttt tttaaatgca gttaaatatg
      181 ataagggaag agtttcatat tttggcgaag attatcagcc tgaaaaaata ttaaatcaat
      241 ccaaagtcga agttttgaaa ggattgtgcc gatttagaaa atttccgggt tcggcgtgta
      301 ataagattat aaaaagggaa ttgattatta aagaaaaact attttttgaa aaaggaattt
      361 atgctgaaga tatcgaatgg tcaatgaggt tgtttaatgc ggcaacaact ttttcttatt
      421 tggacggttg ttattactat tatcggcagg gaagaaaaga ttctattacg ggaactgttt
      481 cggaaaaagg tataaagtca ttattatata ttttggagaa aaatgcggaa atggaattta
      541 atagggatat atcgagttat ctttattctt ttctttccta cgaatatctc gttttgcttt
      601 ttataatgac aagtaaaaat atagcgtgtg attctgatat aaaaagaagg gcgtatcatt
      661 taaggtttat gctgttaaaa tctaataagt tgatatataa gctgatattc ccgataatca
      721 cattattcgg ggtcgatatt acaggcagga ttttaaaagc aatcaggggg aatatttaat
      781 aaatccttta acaatatata ccttaccgaa ggaggaaaaa tgaacgcaat cagaactttc
      841 caaaaccgca cgcccgaaat ccacgaaacc tgcatgatag acgaggcgtg cgtcgtcatc
      901 ggcgaagtct cgcttgccga agatgtttcc gtgtggccgt gcgccgtgtt gcgcggcgat
      961 gtgaacagca tcaccgtcgg cgcgcgcagc aatatacagg acggcagcgt cctgcacgtt
     1021 tcccacaaaa ccgccgccaa acccgaaggg tcgccgctgg ttatcggcga agacgttacc
     1081 gtggggcaca aagtgatgct gcacggctgc cgaatcggta accgcgtctt ggtcggtatg
     1141 ggcagcaccg ttttggacga tgccgttatt gaagatgacg tgatgatcgg cgcgggcagc
     1201 ctcgttccgc cgcgcaaacg tttggagggc ggctatcttt atatcggttc gcctgtgagg
     1261 caggttcgtc tgctgaccga tgaggaaaaa gcctttttga aatattccgc cgcacattat
     1321 gtgaagctgt cgaaacagta cgggatgtga aatcacatcg gcgttcttgc gtcaacccca
     1381 aattcacgcg gttggacgca cccgatgatg attccgatgc gccttgattt tgaccggctg
     1441 cgtttgagtt gcaggcaaaa atgccgtctg aaagcctttt tttcggcttc ggacggcatt
     1501 ttattgccga tttcttttta gagtttgacc gaatgttcgc gcgtttcgtg gaacacgatg
     1561 tccggccaac gttcttgcgt cagtcccaaa ttcacgcgct tggacgcacc cgatgatgat
     1621 tccgatgcgc cttgattttg atcggtgcat ttgaattgca ggcaaaaatg ccgtctgaag
     1681 cccttttttc gggttcggac ggcattttat tgccgatttc tttttagagt ttgaccgaat
     1741 gttcgcgcgt ttcgtggaac acgatgtccg gccagcgttc ttgcgtcaac cccaaattca
     1801 cgcggttggg ggcgaggtag gcgaggttgc cgcctgcgtc gatggcgagg ttgcctgcgt
     1861 tggctttttc aaattccgcc agttttttct tgtcgtcgca cgatacccag cgcgccgacc
     1921 agatggatgc gctgtcgaac acggcttcca cgccgtattc gttggcgagg cgtgaggtta
     1981 cgacttcaaa ctgcaacacg ccgaccgcac ccaaaatcaa atccgcgccg ctcatcggtt
     2041 tgaatacttg aaccgcacct tcttcgccga gttgttgcaa acctttttgc agttgtttga
     2101 ttttcagcgg gtttttgatg cggacgctgc ggaacagttc gggcgcgaag aatgggatgc
     2161 cggtaaacgc cagttgttcg ccttcggaga agctgtcgcc gatttggatg ttgccgtggt
     2221 tcgggatgcc gatgatgtcg ccggcgtagg cttcttccgc cagttcgcgg ttgtgcgaca
     2281 tgaaggttac tacgctggag gcggcgattt cgcggttgat acgcaggtgt ttcatcttca
     2341 tgccgcgctc gaatttaccg gagcagacgc gcaagaaggc gatacggtcg cggtgtttcg
     2401 ggtccatatt ggcttggatt ttaaagataa atccggaaaa tttcggctcg tccggcccga
     2461 ccatgcgcat ggtcgcgtcg cgcggtttcg gtgcgggtgc ccagtcaatc aatgaattga
     2521 ggatttcctg aatgccgaag ttgttaatcg cagagccgaa gaacactggc gtgagttcgc
     2581 cggcgagaaa ttcgtcgaga ttaaattcgt tggaagccgc ctgcaccaat tcgatttcgt
     2641 cgcgcaactg ctggatttcc aacggaaagc gttgttccaa ttcgggattg tttatgcctt
     2701 tgatgatgtc gaactcgtgc ggcaggcgtt cgccgcccgc ttcaaagaga tagatttcgt
     2761 cgttcaggat gtggtacacg cccttgaagt ttttgcccat accgatcggc caggtaacgg
     2821 gtgcgcagcg gatttgcagg atgtcttcca cttcgtccaa gagttccaaa gaatcgcgca
     2881 cttcgcggtc gtatttgttc atgaaggtaa caatcggcgt atcgcgcagg cggcagacgt
     2941 tcaagagttt gatggtttgc gcttccacgc cttttgccgc gtcgatgacc atcaaggcgc
     3001 tgtccactgc ggttaaaacg cggtaggtgt cttcggagaa gtcttggtgt cccggcgtgt
     3061 ccaagaggtt gacggtgtgg tctttgtagt cgaactgcat cacgcttgat gccacggaaa
     3121 tgccgcgctg cttctcgatg tccatccagt cggaggtggc gaatttgccg gttttcttac
     3181 ctttcaccgt gcctgcgctt tgaatcgcgc ccgaaaacag cagcagtttt tcggtcagcg
     3241 tggttttacc cgcatcgggg tgggagatga tggcaaacgt gcggcggcgg cgcacttggt
     3301 cgaggatttc ttgggacatg gttttctttg caaaaaggtt caggccgctt ttcagacggc
     3361 ccggacagtg tttgagacgg cgaaattgta caaaaaaatg cctgataatt caatgttgga
     3421 ggcggtcagt gcgtgctgcc gtaaatctct ttttcgtctt tcaggacggc atcggcggtt
     3481 tcccacgcac cgccgttcca ttttttgtaa aagcagcttt cgcgcccggt gtggcaggcg
     3541 atgccgccgt tttgggcgat gagcatcaca atggtatcgc cgtcgcagtc gaggcgcagt
     3601 tcgcggactt tttgcgtgtg tcccgactct tcgcccttca tccattgttt ttggcgcgaa
     3661 cggctgtaat agtgggcaaa gccggtttcg acggtttttt gcagggcttc ggcgttcatc
     3721 cacgccacca ttaaaacacg tttggtttcg gcatcttggg cgatggcgca aaccaaacct
     3781 ttttcgtcaa atttgacggc ttcaagcagg tttttatcca tatttccttt cagacggcat
     3841 agtcggggcg gtcagaggcg cacttcgatg ccggcttcgc gcatagtgcg tttggcttcg
     3901 cggatggtga tttccccgaa atggaaaatg ccggcggcaa gtacggcatc ggctttgcct
     3961 tcggttatgc cttcaatcag gtgccggaca ttgccgaccc cgccggaggc gatgacgggg
     4021 atgtcgacgg cttcggcaac ggcgcgggtc agcggcaggt tgaaaccctg tttcgtcccg
     4081 tccctgtcca taccggtgag caggatttcg cccgcgccgc gtttttgcat ttcgaccgcc
     4141 cattccaccg catccaaacc ggtcggattg cgcccgccgt gggtaaagat ttcccagcgt
     4201 gtgttttcgg ggttgacggc tttggcatcg acggcggcga cgatggcttg cgaaccgaaa
     4261 aatccggcgg cttcgttaat taaatcggga cgggtaacgg cggcggtgtt gatgctgact
     4321 ttgtccgcgc cggcattgag caggcggcgg atgtcggcaa cggtgcgtac gccgccgccg
     4381 acggtcagcg ggatgaagac ttgtccggca acctcttcga tgatgtgcag gatggtgtcg
     4441 cggttgtcgg atgaggcggt gatgtcgagg aaggtcaatt cgtccgcgcc ttcgccgttg
     4501 tagcgtttgg cggcttcgac ggggtcgccc gcgtcgcgca aaccgatgaa gttcacgcct
     4561 ttgacgacgc gcccgtcttt tacgtcgaga caggggatga tgcgttttgc cagtgccata
     4621 atcggatgcc tttagtcgag ggaatctgcc agttgctgcg cttgggcaaa atcgatgctg
     4681 ccctcgtaaa tcgcgcggcc ggtaatcgcg cctgctacgc catgtttttc ggcggcacac
     4741 agggcgcgga tgtcgtccaa gccggtcagt ccgccggagg cgatgacggg aatgcggacg
     4801 gattgggcga gtttgaccgt cgcgtcgatg ttcacgccgc tcatcatacc gtcgcgcccg
     4861 atgtcggtgt agatgatgct gttgacaccg tcgtcttcaa agcgttttgc caaatcaatt
     4921 acatgatgcc cggttacggt tgcccagccg tcgatggcgg ccataccgtc tttggcatcc
     4981 agtccgacga taatcctgcc ggggaaggct ttgcacgctt cgcgcaccaa gtcggggttt
     5041 ttgactgccg ccgtgccgat aatcacgtcg ttcagtccca aatccaaata ttgtccgatg
     5101 gttttcaaat cgcgcatgcc gccgccgagc tgcacgggga tgtctttggc gacggcggcg
     5161 aggatgtctt tgatggcggg caggttttgc ggaacgccgg caaatgcgcc gttcaaatct
     5221 accaggtgca ggcggcgcgc gccttgttcg aaccagtgca gcgcggtgtc ggcgggcgaa
     5281 tcggaaaaga cggtcgcctc ttccatcagc ccttgtttca agcggacgca acgtccttct
     5341 ttcaaatcga tggcgggtat cagcagcata gtttttctcc ttgtgcgggg ccgtgtccgg
     5401 cttaccagtt taaaaagttt ttcaacatcg tcagcccggc atcgtggctt ttttcggtgt
     5461 gaaattgcgt ggcgaatacg ttgtctttgc cgacgatgca ggcaaacggg gacgggtagt
     5521 cgctttcgcc caatatggtt tcgggatttt cgggggcgaa atagtagctg tggacgaaat
     5581 aaaaacgcgt gttttggggg atgccttgaa aaagcgggtg gttttgggtt tggcgcacgg
     5641 tgttccagcc catatgcggg actttcagac ggcatccctg cgggtcgcgg aggtcgcggg
     5701 caaagcgtct gactttgccg ccgaaccagc ccaagccgtc ggtgtttcct tcttcgctgt
     5761 ggtcgaataa aagttgcgca ccgacgcaga ttccgaaaaa cggtttgttt tttaaggcgt
     5821 ctttgactgc ctcgtccaag ccgccgcgcg tcagtgccgc catacagtcg ggcatcgcgc
     5881 cctgcccggg aaaaatgact ttgtcggcgc gggacacgcg gtcggggtcg ccgcttaaaa
     5941 agattttggt attttttccg gcaagctgcc ccgccgtccg gacggatttc aatacggaat
     6001 gcaggttgcc cataccgtaa tcgataatgg cggtttgcat ggcttcctcc tcttttttgc
     6061 aatatggctg cgattttaac aaacaaatgt gccgggctga taaaaatgcc gtctgaaaac
     6121 gggaatctgt cttcagacgg catagggttc aaacccggaa agccgtttgt cagccttcca
     6181 tttgttttgc ctgaacggca gtcagggcga tggtgaacac gatgtcttcc accagcgcgc
     6241 cgcgagacag gtcgttgacc ggtttgcgca ggccttgcag cagcgggccg acgctcaaga
     6301 cgttggcact gcgttggacg gctttatagg tgcagttgcc ggtgttcagg tcggggaaga
     6361 ccaaaacggt tgcctgtcct gccaccgggc tgcccggggc tttggatttg cctacgcccg
     6421 gcacggttgc cgcgtcatat tgcagcgggc cgtcgatggc gaggtcgggg cgtttttccc
     6481 gggcaagttt ggtggcttcg atgacggtat cgacatcggg gccgctgccg gagttgacgg
     6541 tggagtagga aatcatcgcc actttcgggt cgatgccgaa ggcttttgcg gaatcggcag
     6601 actggatggc gatgtcggca agctgttgcg cggtcgggtt cggattaacc gcgcagtcgc
     6661 cgaacaccag aacctgattt ggtagcagca taaagaatac gctggatacg aggcttgcgc
     6721 ccggtgcggt tttaatcagt tgcaaagcgg ggcggatggt gttggcggtg gtgtgaaccg
     6781 caccggatac caaaccgtcc acatcatttt gcgccatcat catcgtaccg agtaccacgg
     6841 tgtcttgcag ttgcttgcgc gcgtcttcgg gtgtcaggcc tttggatttg cgcagttcgc
     6901 acatcggctc gacgtattgt tcgaccaatg aggcgggatc gatgatttcc aaagagtcgg
     6961 gcaggctgat gccgcgttct ttggcaacgg cttcgacttc ttcgcgtttg gcaagcagga
     7021 cgcagcgggc aatgcctttt tcgtggcaga tggcggcggc ttggacggtg cggggttctg
     7081 cgccttcagg caggacgatg cgtttgccgg cttggcgggc gaagtcgatc aggttgtagc
     7141 ggaattgcgc cggcgacagg cgttttgctt cgcggcctgc caatacggat acgtctttca
     7201 gcgcgtcgct cgaaccgaag aaggtcaggc cggttttctc ggctgccgct tcggcaacgg
     7261 aggctgccgc accgtccacg acaaaacctt ccaatacgcc cggcgcggcg gcgaagaact
     7321 gtttggcaag gttcaattgg tgtgccagcg cgtcggcatc ggtattgtcg gaacggacgg
     7381 cgaagacggc tgccgcgtca agggacaatg ccagttcgac gtttttgcct gcgagataga
     7441 ttttgtcggc atcgggcgcg atgccttcga tgacgaggtt ggcggcatcg agtgcggcaa
     7501 ctttgccgac cagtgcgtcg aaccagtcgt cgcttttgcc ttgcgtgagc agggtttcgg
     7561 cggttgcgtc aacggcttgg aaaacttgtg cgtccagtgc ttttgcaaag gcttgtgcgg
     7621 cggcggaggt gttcagtccg gcagatacgg gtacgacgag tacttttgcc ataatatatc
     7681 ctttcgtatg ctgcggtgtg cggcatatgt ggttggaagg ggcggcatat gggcagaaac
     7741 ggctgcctgc gtgccgtgcg tgccgtgttt ggcttggggc gcgcaggttg aatatagcaa
     7801 acaaattctg tttccaacaa gataaatatc cgcaggcttg tggatgctgc cgcctttcag
     7861 agggtatttc cggggaagaa cagggcggga ccgtccaaat ggaggacggc ggaaatgccg
     7921 tctgacaggg tgggggcgga agggaggttg agcgtgagga cggtttgtcc ggccctgagg
     7981 ctgatttcgg tatgccgcgc tttgggcgtg gttttgagaa ccacggcgtg aatggaggcg
     8041 gcgggtgcgg aatgggggtg aaggctgaac tgttccggac ggatgagcag tgtaccgcgc
     8101 gtgcctgcgg gtgcgccgct ttggacgggc aggcggccca atctgcaatc ggcggtgccg
     8161 tcggcgttga gcgcggcggg gaacacgatg ccttcgccga taaacagggc ggcatcaagg
     8221 tcggcaggtt gtcggtacaa ttcgtgaggg cttgcggttt ggaggatgcg cccctgtttc
     8281 atcacggcaa tccggtcggc gtattgcagg gcttcttcgc ggtcgtggct gacaaaaacg
     8341 gcggatttgc cgttggcgcg cagggcggca atcatgtctt cgcgaatctg gcggcgcaac
     8401 tgttcgtcca gcgcgctgaa gggttcgtcc aacaaaatca gttcggggtc gggggcgagg
     8461 gcgcgggcga gggcgacgcg ctgttgttgt ccgcccgaaa gttcgtgcgg atagcgtccg
     8521 gcaagttcgg aaatgccggt caattccaac atggcttcga tgcgctgtcg ctcttgcgcc
     8581 gtcctgcctt tgccgttgcc gagaccgtag gcgatattgc ggtaaacggt caggtggggg
     8641 aacagcacgc cttcctgtac gaggtaaccc aaacggcgtt cgcggacggg aaggttggta
     8701 tttttcgaga agatggtttt gccggaaagc gaaatttcgc cggaatcggg ttgttcgaaa
     8761 ccggcaaggc agcgtaaaag ggtggttttg ccgcagccgg acgcgccgat gataaagaga
     8821 atttcgcccg ggtcgaggct gagcgaaatg tcgtttaaaa ccggggtgtt ttgaaaactt
     8881 ttggacaggt gtccgatgtg cagggcggcg gtcatagcgg tgcttcctca agctgttatt
     8941 tgaaggcgta tttcttcagc aggaatacgg gtatgccgga aaataatacc agcatcagcg
     9001 cgtaaggggc ggcggcggcg tattgtgcgt ccgatgtgta ttcccaaacg gcggtggaga
     9061 gcgtgtggac atcgtcggcg gtcagcagca gggtggcggt caactctttc atcagcttga
     9121 ggaagacgag tgcgaatgcg gcggtaatgc cgggcaggat ggacggcagt accaacgtcc
     9181 tgaaaataaa gaagtgtccg cgccccaatg ttgcgccgac ctgttccatc cctttgggga
     9241 gttgttccaa ggaagtcctc agggtggttt gcgccatcgg caggtaaagc atgaaatagg
     9301 caaggatgac gacgataaag gtttggtaaa cggcaggggt gtagttgatg ctgaaataaa
     9361 ccaaggatag ggcgataacc aaaccgggga cggcgtgcag taaaaacggc agcctgtcta
     9421 tccaaacggt taaaaaattg cgatagcgaa ccgatgccca aacaaggggc aaggcacata
     9481 atatagtcaa aatcgcacct aaagccgata cgcttaagga acggataaag gcatcaaata
     9541 cggatacgag cgcgaatgtg ccggaagtgc cgaccatcat ccaatgtatc aatacgccaa
     9601 aggggataat aatgcccaaa atcaacaagc tgcttaaaaa aacaatcgcg ccgatctgac
     9661 cgggcagttt gagggttttg acgggataag gacgggcaac gcctttgccg ctgtggtaaa
     9721 tcttggcttt gccgcgaaat atgctttctc caaatacgac gatgccgcac accgccgtca
     9781 aaacagcgga aagcagggcg gcggtattgt tgttgtagga catttcgtat tcttggaaaa
     9841 tggcagtggt aaaagtgggg tagttcaaaa tggataccgc gccaaattcg accagcatat
     9901 gcagggcgat cagtaacacg ctgctgccga tggcgggttt gagctgcggg aggatggcgg
     9961 aaaaaaaggt ttgcaggcgg cttttgccca aggacaggct gacttcttcg taagacaggc
    10021 tgatgcgttt gagtgccgcc tcgacgggca ggtaggcgag cggaaacgag gacaggctca
    10081 taatcatcac tgtcccccaa aagccttcga cgcggaaggt caggctgatc caggtgaaac
    10141 agctgacaaa tgcggggatg cacaaaggca gggtgattgc cgtctgaaaa aaggttttgc
    10201 cgaagaagcg gtaacgttgg aacaaaaggg cgcaggcaat gcccaaaaca atggaaatca
    10261 gggtaacgcc cgccatcatc gtcaaggtgt tggagagcaa atcccacata cgcgggcgga
    10321 acaacagttc gacggcgcgg ttgatgccga cctgccacga acgcatagcg acatataaaa
    10381 aaggcagggt aagcggcagg gcgatcagta ggatgaggcc ggtaagccaa atgggtattt
    10441 ttttaggaga catagtgttt ttcatcggca aaacgggcgg acagtataaa tgtccgcccg
    10501 tttgacaatc cgaaaacggc ttatttcata ccggcttgct caagcagccg ggtggcgtgt
    10561 tctttttcgg aaacagtggt ggcggacact tggggtgctt ccaacttggc gatgggttcc
    10621 aaattgaagg tggataccac gtgcggattc aaaggatatt cggcacggac ggcggtcagg
    10681 gcgcgctgtc cttccttgcc ggcgaggaag gcgacgaatt ttttcgcctc atccttgttt
    10741 tgggaggatt ttaacacggc tgcgccggaa taggtaacga gtgcgccggg atctctgtgg
    10801 cggacgaaat tcaggcgggt gtggacattt tgtacgcctt tttcacgcgc gaaagcgtgc
    10861 cagtagtagt tgttgatgag ggcggcatcg atttcgccgt tttcaaccgc ttgaagggcg
    10921 acggagtttt tagcgtaagg cttgccgtat tctttcagac ctttgagcca tttcaatgcg
    10981 gccgcttcgc ctttcagttt gacgatggcg acaatctgtt ccaagaacgc gccggaagtg
    11041 gggacgtaac cgatgcggtt tttccatttc ggcgtggcgt aattcaggac ggatttttcc
    11101 aaatcttttt cagacagttt gcgggtgtcg taaacgacga cgcgcgaacg tccgctcagt
    11161 gccacccagt cttttttggc ggcaaccggt acgcctttgc cgcgtgtttc gttgatggtg
    11221 gaggcgggca ggggctctag gaggttggct gcggaaaggg tggcgagtgc cgggatttgt
    11281 tcggaataga atacgtcggc ggggcttcgg ctgccttctt ctttgatttg gccggcaagc
    11341 tggtcgcctt tggcactgtt gagtttgact ttgatgccgg tagcccgggt aaaggcatct
    11401 gcaacggctt gtgccgcttc tttgtgttgg ccgttgtaca cggtaatgtc tgccagcgcg
    11461 ggggtggcgg cggtcagggc tgcggcaagc agtgcgtatc ggatagatgt tttcatatcg
    11521 attttctcct aaatgaatga gggtgtatac cttgttaaga cataacgggg tgtagtgtat
    11581 tccttctttt ttataaatgc aaataattat tttttaaatt tgttattatc cgatccggtt
    11641 attgtttgtt ctgacttgta ttttttccgt gcatcgcgcc cgtaaggcgg aagcggcggg
    11701 caatgtgtgg cggaatgtgg gtaaaggcgg cattttgatt tgtcggaatg ctggagaacc
    11761 tctcccttta aaacgccgtc tgaacaaggt tgccggaata gtattgccat cccggcagat
    11821 acagtttgtc gggatcttgc caatattgtt catccagact gttcggcagc gaggcggttt
    11881 tgttgtcgag atgttttgtt aatccactat atatcccaca tttcttttag gtttttacct
    11941 tccgattgga ggtggcggat ttcttttccc aatgcgtctg ccgcccgatg gatcagcatt
    12001 gcgctgtgta ccgcgccgat ttttttcaga acggaacaag tcttttcggc ggcgggaaga
    12061 ccccagttgc agacaaattg cagtatgccg ccgttgccga tgtcggcttc cgcccgccaa
    12121 atcaaaacca gctcctgttc ccgtccgtcc atgctttcga gtttgccgtc atgctgttca
    12181 aaaagtttgt ccaccgcctg ccgcatcatc tgctcgaagc ggtcggcttg ggtatccgta
    12241 tcggtcatag tattcttgcc ttttaaaaat gccgtctgaa catttcttca gacggcattt
    12301 gggggttaag ctgacatttc ccgccagcgt ttgacttgga ggcgtacctg ttcgggcgcg
    12361 gtgccgccca agtggttgcg cgcgttgagg ctgccttcgg gtgtcagaac gccgtacacg
    12421 tcgtctgaaa tcagcttgct gaagccttgc agggcttcga gcggcaattc gctcaaatca
    12481 acgccggctt catcggcgtg gcgcacggct tgggcgacga cttcgtgggc atcgcggaag
    12541 ggcatacctt ttttgaccag ataatccgcc aagtcggtgg cggtggcaaa accctgcatc
    12601 acggcggcgc gcatattgcc gggtttgacg gttacgccgc gcatcatatc ggcgtaaatc
    12661 cgcagcgtgt cgatgagcgt gtcggcggtg tcaaacagcg gttctttgtc ttcctgattg
    12721 tctttgttgt atgccaaggg ctgcgatttc atcagggtaa tcaggccgat aaggtgtccg
    12781 atgactcggc cggatttacc gcgcacgagt tcgggtacgt cggggttttt cttttgcggc
    12841 atgatggacg aacccgtaca gaagcggtcg gcgatgtcga taaagccgaa acgcggactc
    12901 atccacaaaa tcaattcttc agacaggcgg ctcaagtgaa ccataaccag cgaggcggcg
    12961 gcggtgaact caatggcaaa atcgcggtcg gatacggcat cgagcgagtt ctggcagatt
    13021 tgttcgaaac ccaacaattc ggcggtgatt tcgcgctgaa tcggataggt cgtaccggca
    13081 agggcggcag cgccgagcgg catacggttg acgcgtttgc gacagtcggc catgcgttcg
    13141 aaatcgcgtc cgagcatttc gacgtaggcg agcaagtggt gtccgaagct gaccggctgg
    13201 gcgacttgca gatgggtaaa accgggcatg acggtttcgg cgttttgttc cgccaaatcc
    13261 aataatgccg tctgaaggtt ttgaatcagg ttttggataa cggtaatctg gtcgcgcagc
    13321 caaaggcgga tgtcggtggc gacttggtcg ttgcggctgc ggccggtgtg caggcgtttg
    13381 cccgcgtcgc cgattttgtc ggtcaggcgg cgttcgatgt tcatgtggac atcttccaaa
    13441 tcgagcggcc attcgatttt gccgctgcgg atttcttcga ggatttccga catgccccga
    13501 cggatgtcgg tcagatcgtt ttcactcagc acacccgacc gggtcagcat ctgcgcgtgg
    13561 gcgagcgagc cttggatgtc ccattcggca aggcgttggt cgaaaccgat ggaggcggtg
    13621 tattgtttga cgagttcgga aacgggttcg ttgaaacgtc cggaccaggt tttgtcgtgc
    13681 ataaggattc cttgatgggg ttattcggtg cggtattttt ccaaaaggcg gcggaagggt
    13741 tcgccggttt cggggtgttt taaaccgtag gcgacggtgg cttcgagata gccgagtttg
    13801 ctgccgcagt cgtagcgcgt gccttcaaag gggtgcgcca agacaaattc gtgatcgagc
    13861 agcttggcga tgccgtctgt aagctggatt tcgttgcccg cgccgcgcgg caggccggtt
    13921 aaaaggtcga aaatgcgcgg ggtgaggatg tagcgtccga caacggcaag gttggagggc
    13981 gcgtcttcgg gcttgggttt ttcgacaatg ccggtaatgc gttggaactg tttgagctgt
    14041 tcggtttcga cgatgccgta tgagccggtt tgcgacggtt cgacggtttc tacgcccaaa
    14101 atgctgttac cgctgcgccc gtacacttcg accatttgtt tgagcgcgcc tttgggcgca
    14161 tcgattaagt cgtcggcaag gatgacggcg aagggttcgt cgccgatggc ggcgcgggcg
    14221 cacaagacgg cgtgtcccaa tcccagtgct tccgcctgac ggatgtagag gcaggtaatg
    14281 ttcggcggca ggatgttgcg gacgtgttcc aacaatttgt ctttatggcg catttccaac
    14341 ttagtttcga gttcgtatgc cttgtcgaaa tggtcttcga tgctgcgctt gttgcgcccg
    14401 gtaacaaaca ccatttccgt gcagccggct tccacggctt cttctacggc gtattggatc
    14461 agcggcttgt cgacgatggg cagcatttct ttcgggttgg ccttggtggc gggcaggaag
    14521 cgggttccca tccctgcaac ggggaagacg gcttttttta tcggtttcat tctttttcct
    14581 ttgtattgtt ttgatgttta aagggcgagt ttgcgaatta aatcggcgag tgcctgcgcg
    14641 cggtggcttt cgcggttttt gacctccgaa tccaattcgg cggcggtttt gccgtgttcg
    14701 ggcagataaa aatacgggtc gtaaccgaaa ccgttttgcc cgagcggcgt gtcgtgccat
    14761 tgtccgtgcc atatgccctc ggcgataatc gggcgcgggt cgtctttatg acggacaaaa
    14821 accaatacgc agacataaga acaacttttg tcggccttgc cggcaagttc ggcggcaagt
    14881 ttcaggttgt tggcggtatc ggatttggga ttgctgccgg cgtaacgtgc ggaatggatg
    14941 cccggcgcgc cgtttagggc ggcggcacag atgccgctgt cgtcggcgag tgcgggcagc
    15001 ccgctgtgtc cggcggcgtg ccgggctttt gccagcgcgt tttcgacaaa ggtgggatag
    15061 ggttcggggc attcgggtat gccgaatgcg gattgcggca atacggtgat gccgtaaggt
    15121 ttgaataagt tgccgaattc tttgagcttg ccggcattgc cgcttgccaa aacgattttt
    15181 tccggttttt cagacatagc ggtttccctg tgatgaagat ggggcggcgc gtagggattt
    15241 gtgccgcagg tagagggcga gtgcgccgat ttgcccgaac agcgcgccga atgcgaaaag
    15301 gaaggacgcg acggcgaagg ctttgctgcc ggcggtcagc agataagcac ccaagaggac
    15361 gggcagcata aatgccagtg tgaacaaggc aacagacaac agatagattt ttcggcggtt
    15421 catggcgttc ggtcggaaac gggatgttcg gattatagcc gattaggacg gcattcccta
    15481 gaggctggaa aaatgccgtc tgaagcggcg ttcagacggc atggcgggca ttatgcctgt
    15541 ttgttccaac gttcgatgga ttctttgatg acttttttcg cttcttccgc accgccccag
    15601 gattcgactt tggtcgtacc cgcttttttc aggtctttgt agtggttgaa gtggaactcg
    15661 atttgtttga tgagctgttg cggcaaatcg gacaaagttt tgtaggcgtt gccgttattg
    15721 cggtcgtcgg caggtacgca gacgattttg tcgtccactt cgccgtcgtc gacgaatttc
    15781 atcacgccga taacgcgcgc ttccaagaat acgccggttg ccagaggttg ttcggtaacg
    15841 agcaacacgt ccaattcatc gccgtcttcg tccaaagttt gggggatgaa gccgtagttg
    15901 gtcggtttgg cgaagatggc gggttcgatg cggtcgagtt ggaatgcggc gagtttgcgg
    15961 ttccactcga ttttgtggtt gctgccggcg gggatttcgt tgacgacgtt gatgatgccg
    16021 ccgtccacgt cgcccggggt caggatttgg ttgaagtctg ccatttggtt tcctttattt
    16081 gggaaggttt gaagtttgaa agtatagcac aaacgtccgg ctggaaatgc gtccgatgcc
    16141 gtctgaaagg gaaaccgggc gcgtgttacc gtttgcccaa aaacctgccc agttccaaaa
    16201 tcgcgcgcct gttggacggg gaaaacactt tttccgccgc ttcttccaag ccgaaccagc
    16261 cgtaggagac gtgttcctcg ggttgcagga cgacgggcgt atcgcgcggg atttcggccc
    16321 ggaagacgtg ttcgcggttt tcaaacacgc ctttggggta gcggtgccgc cagtggtggt
    16381 agatttcgta aaccgtgctg tcgtgccggt cttggagctg cccgtcctcc agcaggatgc
    16441 cggtttcttc ccaaacttcg cgccttgccg tttgggcgac ggtttcgccc ggttcgaggc
    16501 tgccggttac cgactgccaa aatcctttcg gatgcgtgcg ttcgatgagc aggatgccgc
    16561 cgtccccgct ataaaggacg accagtgcgg aaacggggta tttgagcggt tttgccatcg
    16621 gcatctttcg gcgggctgcg gtaatgaagg ggcggattat agcaaacgcc gcacgttatg
    16681 gcgtttatcc ttttccgtat ccttttttct gcacggatgg gacgcgccgg tgtttgccgg
    16741 taaattttcc tttgtgtcaa aaagataagg gcggttgtga ttttaatgct tgccaaagtg
    16801 acgggcggaa actataatcc gcgacttacc gagtcggagt gtggcgcagt ctggtagcgc
    16861 acttgcatgg ggtgcaaggg gtcgaaggtt cgaatccttt cactccgacc aaaaattccg
    16921 aaagccgctt tcaaaagcgg cttttttgcc gtccgtatga ttatgatgta gagtacgcgg
    16981 cgacagacat tcaaatgccg tccgaaaacc gttcagacgg catctcttta tcttaatagt
    17041 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggcaag
    17101 gcgaggcaac gccgtactgg tttaaattta atccactata gtttcattcc gtaccatctt
    17161 aaggaacatc aaattgggca tttcccgcaa aatatccctt attctgtcca tactggcagt
    17221 gtgcctgccg atgcatgcac acgcctcaga tttggcaaac gatcccttta tccggcaggt
    17281 tctcgaccgt cagcatttcg aacccgacgg gaaataccac ctattcggca gcagggggga
    17341 gcttgccgag cgcagcggcc atatcggatt gggaaacata caaagccatc agttgggcaa
    17401 cctgatgatt caacaggcgg ccattaaagg aaatatcggc tacattgtcc gcttttccga
    17461 tcacgggcac gaagtccatt cccccttcga caaccatgcc tcacattccg attctgacga
    17521 agccggtagt cccgttgacg gattcagcct ttaccgcatc cattgggacg gatacgaaca
    17581 ccatcccgcc gacggctatg acgggccaca gggcggcggc tatcccgctc ccaaaggcgc
    17641 gagggatata tacagctacg acataaaagg cgttgcccaa aatatccgcc tcaacctgac
    17701 cgacaaccgc agcaccggac aacggcttgc cgaccgtttc cacaatgccg gcgctatgct
    17761 gacgcaagga gtaggcgacg gattcaaacg cgccacccga tacagccccg agctggacag
    17821 atcgggcaat gccgccgaag ccttcaacgg cactgcagat atcgtcaaaa acatcatcgg
    17881 cgcggcagga gaaattgtcg gcgcaggcga tgccgtgcag ggtataagcg aaggctcaaa
    17941 cattgctgtc atgcacggct tgggtctgct ttccaccgaa aacaagatgg cgcgcatcaa
    18001 cgatttggca gatatggcgc aactcaaaga ctatgccgca gcagccatcc gcgattgggc
    18061 agtccaaaac cccaatgccg cacaaggcat agaagccgtc agcaatatct ttatggcagc
    18121 catccccatc aaagggattg gagctgtccg gggaaaatac ggcttgggcg gcatcacggc
    18181 acatcctgtc aagcggtcgc agatgggcgc gatcgcattg ccgaaaggga aatccgccgt
    18241 cagcgacaat tttgccgatg cggcatacgc caaatacccg tccccttacc attcccgaaa
    18301 tatccgttca aacttggagc agcgttacgg caaagaaaac atcacctcct caaccgtgcc
    18361 gccgtcaaac ggcaaaaatg tcaaactggc agaccaacgc cacccgaaga caggcgtacc
    18421 gtttgacggt aaagggtttc cgaattttga gaagcacgtg aaatatgata cgaagctcga
    18481 tattcaagaa ttatcggggg gcggtatacc taaggctaag cctgtgtttg atgcgaaacc
    18541 gagatgggag gttgatagga agcttaataa attgacaact cgtgagcagg tggagaaaaa
    18601 tgttcaggaa acgagaagaa ggagtcagag tagtcagttt aaagcccatg cgcaacgaga
    18661 atgggaaaat aaaacagggt tagattttaa tcattttata ggtggtgata tcaataagaa
    18721 aggcacagta acaggagggc atagtctaac ccgtggtgat gtacgggtga tacaacaaac
    18781 ctcggcacct gataaacatg gggtttatca agcgacagtg gaaattaaaa agcctgatgg
    18841 aagttgggag gtgaaaacga aaaaaggtgg gaaagtgatg accaagcaca ccatgttccc
    18901 aaaagattgg gatgaggcta gaattagggc tgaagttact tcggcttggg aaagtagaat
    18961 aatgcttaag gataataaat ggcagggtac aagtaaatcg ggtattaaaa tagaaggatt
    19021 taccgaacct aatagaacag catatcccat ttatgaatag taatatttat gaaaaattag
    19081 gagattaatg atgaaaagaa ttaagtgctt ttgt
//
LOCUS       DAXFWM010000044        18905 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000044 DAXFWM010000000
VERSION     DAXFWM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.044"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..258)
                     /locus_tag="ACFI8B_001391"
     CDS             complement(<1..258)
                     /locus_tag="ACFI8B_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834596.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFI8B_001392"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFI8B_001392"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC3834597.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="ACFI8B_001393"
     CDS             3148..4278
                     /locus_tag="ACFI8B_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3834598.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="ACFI8B_001394"
     CDS             complement(4319..5221)
                     /locus_tag="ACFI8B_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC3834599.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5360..5857
                     /locus_tag="ACFI8B_001395"
     CDS             5360..5857
                     /locus_tag="ACFI8B_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFC3834600.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFI8B_001396"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFI8B_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC3834601.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7288
                     /locus_tag="ACFI8B_001397"
     CDS             6953..7288
                     /locus_tag="ACFI8B_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC3834602.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7419..7889
                     /locus_tag="ACFI8B_001398"
     CDS             7419..7889
                     /locus_tag="ACFI8B_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834603.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFI8B_001399"
     CDS             7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFI8B_001399"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC3834604.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVAAVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            8639..8714
                     /locus_tag="ACFI8B_001400"
     tRNA            8639..8714
                     /locus_tag="ACFI8B_001400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8672..8674,aa:Gly,seq:gcc)
     gene            8740..8815
                     /locus_tag="ACFI8B_001401"
     tRNA            8740..8815
                     /locus_tag="ACFI8B_001401"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8773..8775,aa:Gly,seq:gcc)
     gene            8841..8916
                     /locus_tag="ACFI8B_001402"
     tRNA            8841..8916
                     /locus_tag="ACFI8B_001402"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8874..8876,aa:Gly,seq:gcc)
     gene            8942..9015
                     /locus_tag="ACFI8B_001403"
     tRNA            8942..9015
                     /locus_tag="ACFI8B_001403"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8974..8976,aa:Cys,seq:gca)
     gene            9116..9205
                     /locus_tag="ACFI8B_001404"
     tRNA            9116..9205
                     /locus_tag="ACFI8B_001404"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Leu,seq:taa)
     gene            9309..10361
                     /gene="sohB"
                     /locus_tag="ACFI8B_001405"
     CDS             9309..10361
                     /gene="sohB"
                     /locus_tag="ACFI8B_001405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC3834605.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(10412..11266)
                     /locus_tag="ACFI8B_001406"
     CDS             complement(10412..11266)
                     /locus_tag="ACFI8B_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC3834606.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFDERQLYRHYGLVSRLRVLISNREAFGYLCVGAAMG
                     ILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWS
                     ERNIKAGT"
     gene            complement(11277..11708)
                     /locus_tag="ACFI8B_001407"
     CDS             complement(11277..11708)
                     /locus_tag="ACFI8B_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC3834607.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(11943..12813)
                     /locus_tag="ACFI8B_001408"
                     /pseudo
     CDS             complement(11943..12813)
                     /locus_tag="ACFI8B_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(12889..13800)
                     /gene="hisB"
                     /locus_tag="ACFI8B_001409"
     CDS             complement(12889..13800)
                     /gene="hisB"
                     /locus_tag="ACFI8B_001409"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC3834608.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(13817..14896)
                     /gene="hisC"
                     /locus_tag="ACFI8B_001410"
     CDS             complement(13817..14896)
                     /gene="hisC"
                     /locus_tag="ACFI8B_001410"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC3834609.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(14942..16231)
                     /gene="hisD"
                     /locus_tag="ACFI8B_001411"
     CDS             complement(14942..16231)
                     /gene="hisD"
                     /locus_tag="ACFI8B_001411"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC3834610.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(16228..17115)
                     /locus_tag="ACFI8B_001412"
     CDS             complement(16228..17115)
                     /locus_tag="ACFI8B_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC3834611.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(17207..17872)
                     /gene="hisG"
                     /locus_tag="ACFI8B_001413"
     CDS             complement(17207..17872)
                     /gene="hisG"
                     /locus_tag="ACFI8B_001413"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC3834612.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(17951..18604)
                     /locus_tag="ACFI8B_001414"
     CDS             complement(17951..18604)
                     /locus_tag="ACFI8B_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC3834613.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
BASE COUNT         4273 a         4603 c         5252 g         4777 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gcatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggctaa aacggcttcg ttgacgcggc gttcgacttc agcgatttct tcggcagtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaacg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagtggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacacat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcgggt cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc aaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgcgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggcaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatac cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaac acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggtat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccgccaa agccggtgcg accgacagcg agatcgcagg
     7201 tgcattggca accgccattg ccctgaatgc gggtgctgct tacacttacg ccctgcgtgc
     7261 attggaagcg gttgaaacgc aaaaataatc cgtttcggat aagaaatgcc gtctgaaaat
     7321 atttcagacg gcattttgcc tgttatgctt cccgatattt tgcagacaac atatgcagcc
     7381 gtttttttca gacggcattt gagaaacatg gaacaagtat gaaatatcac cgcttggcat
     7441 tgtttgccgc cataagctgc ctgttgttgt ccgccgtttt tattgcacct tacctgacgg
     7501 catttcacga acaagaaaaa atatttgaat atgcggattt gaccgttacc gcccccaacc
     7561 gcagcggacg ggcaatcaaa ttggaggcgg acgggcggca gtaccggctt tcctgctacg
     7621 ggttcgacag tttgtgcaca ggcggcaata tcggcagagc catcagggcg cggcaggtta
     7681 agattgtttt aagcgaaacc gtcggcaaag gttttttaaa cggcgttctg ttggaatacc
     7741 gcaacagtgg cagtgtctat agcaataaag atttttcccg cacggaagac cgccttgtcg
     7801 aagtgttggc acaacctgcc gttttcagcc tgaaaccggg tattttgctt ttattgcccg
     7861 ccattttcct gcgtttgaaa aaaatgtgaa aacagattgg cggggcgggg gaatgactat
     7921 gcggcaattt tacgttttgg gttatgagga taaccgttat aatcataatc ttacagtccg
     7981 cacagagaaa accgatgcct tggaacatcc ccatcttcct cacatggttg agggtcttgc
     8041 tcattcccgt actgattgtc cttttctacc tgccgttctc atggttttca gaagaagcag
     8101 tcaatgttgc cgccgccgtc atttttgccg ttgccgcctt gaccgactgg tttgacggat
     8161 ttttggcaag gttgtggaaa cagacttccg atttcggcgc attcctcgac cccgtcgccg
     8221 acaagctgat ggttgccgta tcgctgctgc tgctggtcaa actcgaccgg acctatgttt
     8281 tgttcgcgat gattatcatc ggcagggaaa ttaccatttc cgcattgcgc gaatggatgg
     8341 cgcaaatggg aaaaaggagc agcgttgccg tcgccgccgt cggcaagttc aaaaccgccg
     8401 cgcaaatgct ggcgattttc tttttgctgc tgaattttcc cgatttttac ggatttaatc
     8461 ttgttgttat cggcaacata ttgatgttta tcgcatcttt gctgacagtc tggtcgatgc
     8521 tgtattatct gaaaatggcg tggaaagaaa ttgcctgaaa aaaacataaa aatagcttga
     8581 cggtaaaaac ataatccata ataattgcgt cttcttcgat gtcggagagt gaaatcgggc
     8641 gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgagact
     8701 cgtttcccgc tccaaaaatt tcattcttcc gtaatcattg cgggaatagc tcagttggta
     8761 gagcgcaacc ttgccaaggt tgaggtcgcg agttcgaggc tcgtttcccg ctccaaaaat
     8821 ttcattcttc cgtaatcatt gcgggaatag ctcagttggt agagcgcaac cttgccaagg
     8881 ttgaggtcgc gagttcgaga ctcgtttccc gctccaagtt ttgtagacaa gccagtttta
     8941 gggcgagata gcaaagtggt tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc
     9001 cgactctcgc ctccatatat cttacggcgg ggtggcagag tgtttatgct atgaggggtg
     9061 caaccttcat ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg
     9121 ggtggtgaaa taggtagaca caacggactt aaaatccgtc gggactaaac atcccatgcc
     9181 ggttcgattc cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt
     9241 tttttatctg tcggatgggg atggacaata ttgatatgtt catgcctgaa caagaggaaa
     9301 tccaatcaat gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta
     9361 ccgtgttcgg cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg
     9421 aaagcggcag tgtcgtactg acagattttt cggaaaatta taaaaaacag cggcaatcgt
     9481 ttgaaacatt ctttttaagc gaggaagaga caaaacatca ggaaaaaaaa gaaaagaaaa
     9541 aggaaaaggc ggaagccaaa gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg
     9601 ccgaaacgca aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg
     9661 ccgtagaatc cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg
     9721 aggttctgct cagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt
     9781 cgcagcttag gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg
     9841 cggcaagcgg cggctatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg
     9901 cgattatcgg ttcggtgggt gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa
     9961 aacatgatat tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta
    10021 tgggtgaaaa tacggaaaag ggcaaacaga aattccggca ggaactggag gaaacgcatc
    10081 agttgttcaa gcagtttgtc agtgaaaacc gccccgggtt ggatattgaa aaaatagcga
    10141 cgggcgagca ttggttcggc cggcaggcgt tggcgttgaa cttgattgac gagatttcga
    10201 ccagtgatga tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc
    10261 aggagaagcg aagcctgatc cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt
    10321 tgtttgccaa acttgtcaac cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa
    10381 tgccgtctga agtgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttccg
    10441 accactctat ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca
    10501 catcgtccaa actcatggca aacatccaca gataagtgcc gaccgaataa atatgccccg
    10561 cgctgccgta gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg
    10621 ccgcgccgac gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg
    10681 aaaccagtcc gtaatggcgg tacagctgcc gctcgtcgaa gtggttgtcg cgttccaagc
    10741 tgttgttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
    10801 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
    10861 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
    10921 aaaagctgac aaattcacgc gacagggcaa cccgtgcagt taccgctgaa tgcgggactt
    10981 gccgctgccg ttgttccaac acaaccggca cggcgatttc ggtataaatc cgcgtaaacg
    11041 tgcgcgtatc ggcaatccgc cgtgccgcac cgaccagcca catcaaaaat acaaccaaag
    11101 cgtacagcaa cgcctgccac accctccccg caatcacggc attaatcgcc cagccgccaa
    11161 acaccggata ccccagcatc aaaaggtttt ccagtccgac cggggaaaat gtgccaatca
    11221 atcgcttgcg gtgggttttg gctatgtgtt tcaacatttt ccacatattt gtccctctat
    11281 tttttatttt ccgagattgt tttttccatc acttccgcca gtgcatccaa atcggcaaac
    11341 agccgggttg tgcgcttgtc gccgaatgtg gaaaatactt tgtcgctgaa ttcctgcgcg
    11401 ttttctgtta aaggtgcggc atggactttg cctttttcgg tcagcgacag caaccgttcg
    11461 cgcctgtcct gttcgccttc ctgccattca atcaaccctt gtccggcaag ggttttgcat
    11521 acgcctgaaa cggtctgttt gggcagactc cactcttctc cgatgtgctt ttgcgtgcgg
    11581 ctgccttcgg ttgccagggt ataaagtacg gtaaagaggt tgtaattcaa atcctgctgc
    11641 ccgatccatt tgtcaaagac attgcaaatc aggttgatac ggattccaag ttggtcgagt
    11701 cggttcataa ttggtcttga tattgactaa atttgtgcgg attatagtgg attaacaaaa
    11761 accggtacgg cgttgcctcg ccttgtcgta ctatttgtac tgtctgcggc ttcgtcgcct
    11821 tgtcctgatt tttgttaatc cactatatag tcataatcgg gactgatttc aagcgtgggg
    11881 cgataaaaat gccgtctgaa acgctttctg gcttcagacg gcatattgtt tggaaaggga
    11941 agtcagtgtt ctgccaattt caggtaaacg ccggaaacgt cctgttcgcc gtagccggct
    12001 tcaactgctt tgcggtagct ggcagcaacg gtttcgacgg cgggcagggt gttgcctgcc
    12061 tgttcaagtt ctttgacggc gaggttaagg tctttggaag cgtgtttgag tgcaaaggca
    12121 gggggggaac tcacggtttg cccatagtga tttttttgtt tgaaacatag gcgagtccat
    12181 tgccgagccg ccgatggctt cgacgatggt gtcggtatcg atgccgaact gccgcgccat
    12241 cagcatcgct tcgctgtacg cttcgccgaa aatgcctaag agcgagttca agacgagttt
    12301 cgcgcccgag cctttgccga catcgccgaa atggaaggtt tttttgccga caagggaaaa
    12361 tattttttgc agcgggttta aaacggcttc gctgccgccg aacagaatca gcagtgtgcc
    12421 gttggtggcg ggtccgaccg atccggaaac gggtgcttcg gcaaactgtc cgcctgcggc
    12481 ttcgacaagt gctttgacgg cgaggttttc ggtcggggag atggtgctca tgttgacgat
    12541 gattttgccg gccaatccgt cgcggactcc gttcaggatg tcgcacacgg cggcatagtc
    12601 ggaaaccatc aggaaaatga cggggcaggc gcggacgagt tcggcggtgc tgccgtaaac
    12661 ttttgctcct ttggcggaga tgggggcagt tttgtcgggc gagcggttgt atacgccgac
    12721 ttcgatgccg ccgtccaaga gccgcgttac cataggcaga cccatttgcc ctaagcctac
    12781 ccagccgatt tgtgtgtatg tttctgcgga catagtgttt ctcctttgtt ggggcgctgt
    12841 gatgccgtct gaaggcttcc tgcgcttcag acggtctgtt tggggatgtt atgcggtcag
    12901 cgtgcctttg gtcgaggggg tttgcccagc cattctcggg tcgtgttcga ctgccatacg
    12961 cagggcgcgc ccgaaggctt tgaatacggt ttccgcctga tggtgggcgt ttttgccgct
    13021 gaggttgtcg atgtgcaggg tcatcatgct gtggttgacg atgccgtgga aaaattcttc
    13081 aaacaaatcg acatcgaaac gtccgattag tgcgcgggta aattcgatgt tgtacacgag
    13141 tccggggcgg ccggaaaggt cgatgacgac gcggctgagg gcttcgtcga gcgggacgta
    13201 ggaatgtccg taacggcgga tgcctttttt gtcgccgagt gcctgccgga ttgcttgtcc
    13261 gagtgtgatg ccgatgtctt cggcggtgtg gtggtcgtcg atgtgcaggt cgcctttgca
    13321 gctgatgtcg atgtcaatca tgccgtggcg ggcgatttgg tcgatcatgt gttcgaggaa
    13381 gggaacgccg gtatccagcc tgcttttgcc gctgccgtcg aggttgatgg agacggtgat
    13441 ttgggtttcg caggtattgc ggttgacggt aacgctgcgg cagccggcag cagatgcggt
    13501 ttcggcaatt cgggtttcgg cagttccggt gctttctgcg gcggtttcgg ggacagtgcg
    13561 ttcgcggtgt tttctgtcga gccagccttt gggtttgccg gtgtgttttt cgagttttgc
    13621 catcaggctg ggacggatgc cgcgcgcatc ggggtcttct gcctgctttt caaggcgttg
    13681 tttgagtttg taaagtgcga cgggggtacg gtagccgcag agtttggcaa gcttgggcag
    13741 cgaacctgct tcttgggcga gagtcaggaa gttgttcagg tggagttgtg tcttggtcat
    13801 agggattccg tttggtttat cggtagagcc ggcggataac gtcgaggacg gcatcgtttt
    13861 gtgcggagct gcctatggta atgcgcaggc agtgttccaa aagcggatgt gcgccgtgca
    13921 gttttttgac gaggatgcgg ttttgtttga gtgtgtcaaa caacaaatcg gcatcgggta
    13981 cgcgtatggt aatgaagttt gcctgacttg gaaaggcgtt cagacggcat attttgccca
    14041 attcggcgaa catccgttcg cgttcgtttt tcaggctgtc gatgttggca gaaatgatgc
    14101 cgtagtgttg cagggcgagt ttggcggtgg tcaggctcag ttggttcata ttgtagggcg
    14161 gcaggatttt ttgcagttcg ccgatgactt cgggacagcc tgtcgcataa ccgatacgca
    14221 gtccggcaaa accgattttg ctgagggtgc gtaagactat caggttggga atcctgcccg
    14281 cctgcggcag gaagctgtcg ccgttgaatg cgccgtaggc ttcgtcgacg acgacgatgc
    14341 cgtctgaagc ttcgatggcg gcttcgattt cggcacgcgt gaaacatacg ccggtcgggt
    14401 tgttcgggta ggcgataaag gtcagggcag ggcggtgctt cctgacggct tcgaggacgg
    14461 cgggcaggtt gagggtgaaa tctccgttca gtggaacgcc gacataatcc atgccgtaca
    14521 gcgcggcgtt gtggcggtac atgatgaaac cgggttcggc tgccaacatt gccgcgccgg
    14581 gtttggcggt cagcatggtg atgaactgta tcagttcgtc cgaaccgttg cctaaggcga
    14641 cggcggcgca gtcagggatg tcgaacgccg aacgcaatgc ttcctgcaaa ccgcagccgg
    14701 aggggttggg atagaggtgg atgggggcgg atgccagttg cgcccgccat tcccgcatca
    14761 gggcttcgtg cccctcaaaa ggatgggcgg ggctttccat cgcatcgagt ttggcaaaac
    14821 cgggcggaac atcggtgatt ttgtatgcgg acatggcgag gatgtcgttg cggatgaagg
    14881 agcggacgga tttcatcgtg tttccttaat ggttggaata tgcctgtacg ccgtttcggc
    14941 attatttcat acggaactct gccgcgcgtg cgtgggcggt caggctttcg ccgtgtgcca
    15001 gcacgctggc ggtttcgcct aatttttgcg cgccctgttc cgaaacctga atcaggctgg
    15061 agcgtttttg gaaatcatat gtccccaaag gcgaggaaaa gcgggcggtt cggctggtgg
    15121 gcaacacatg gttcgggccg gcgcagtagt cgccgaggct ttcgccggtg tagcgtccca
    15181 tgaaaatcgc gccggcatgg cggatttttt tcgcccattc ctgcgggttt tcgactgaca
    15241 gttccaagtg ttcgggggaa atgtagttgg agatttcgca ggcttcgttc aagtctttaa
    15301 cgagtatcat cgcgcccctg ttgccgagcg aggcctcgat gatgtcgcgg cgcggcatgg
    15361 tttcgatgag cctgtccata gcggcttcta cttcgtcgag atacgcttgc gacgtgccga
    15421 tgaggatggc ttgggcaatt tcgtcgtgtt cggcctggct gaacaaatcc atcgccaccc
    15481 aatcggcggg tgtcgtgccg tcggcgatga ccagtatttc agacggcccc gccaccatgt
    15541 cgatgccgac cacgccgaac acgcggcgtt tggcggcggc gacgaaggcg ttgcccggac
    15601 cggtgatttt atcgacttgg gggatggttt ccgtgccgta ggcgagggcg gcgatggctt
    15661 gcgcgccgcc gacggtgaag actttggtta cgccggcgac gtatgcggcg gcaagcacga
    15721 tgtcgttgcg ttcgcctttc ggtgtcggta cgaccataat gatttctttc acgcctgcga
    15781 cgtgggcggg catcgcgttc atgatgacgg aactcggata tgccgccttg ccgcctggga
    15841 cgtaaatgcc gacgcggtca agcggggtaa tctgttgccc cagcagcgtg ccgtcttcat
    15901 cggtgtagct ccacgattcc attttttggc gttggtggta gctttcgaca cggcgggcgg
    15961 cggtctgcaa tgctgtctga acgtcgttcg gaatgcgctc gaacgccgcg ttcaaatcgg
    16021 cttgcgtgag tattaaatca tcgatgcttt tagcgtttgt ctgatcgaat ttgttggtgt
    16081 attcaatcaa agccgcatcg ccgcgctttt gcacgtcggc gcaaatgtcg gcgacgatgc
    16141 gttcggtttc ggggttttgc gcggtttcaa aagccagcag ggctttgagt ccggcttgga
    16201 aatcgggcga ttgggtgttg agttttttca tgatttggga ttctcctttg ttattttggg
    16261 acttgggatc gtctgaagcg tctcttgctc ggttttgggt ctgatatacg cttttcctcc
    16321 cccgctacca aacttcaatt cgggcaggca tttttgttgc agaccgagtt tggctaatac
    16381 ctcttcgcta cgagaggtag tacggaaacg gtaacgggtt tgttttctgc cgtttttggt
    16441 ttcctcagtc attgtttcaa aaccgtccac ttcgtacgca ctgacaacag cgttttgcag
    16501 accggtgtga acgccgataa cgtatttcac ttttgaagca acatctttac cgataaccca
    16561 gttgcccaac gtacgcgatt tgaggttggc atcgtcttgg ttgtcgaaaa ggtagtctaa
    16621 ttcttcgtca gtatctaaat cgaaagcatt gtggattttg atggcgagaa tcagcccgtc
    16681 ggggttaatc tcgctaagtg ggagagaaga gaatccaaag cggcgatcta gttcttctac
    16741 gctaatacca cccggaccat gcccggcaat tttatttttg agttttttac ccaagacaga
    16801 tttaacaaaa tgaattaagg cagattcggc ggcgagcgct tcgacttcag tcagatgata
    16861 gctgatgata tagcgaccga gtttcttgca tttggagatg gctttgagtt tccgatcgat
    16921 aatctcgccg gagactgggt cttgtgaacg actagcaacc cattcatgct cgaagatacg
    16981 attaccgcaa ccttttccga tgtagaggat tttgtctttt ttcaggtcgg tcagacaata
    17041 gacataaaac cgacgctcgc cgttattcaa aacggaaagg gtcgaatctg agaatttttt
    17101 tatttttgca accattttaa cttctcctat acagtttact gccggtgata ttttcagacg
    17161 gcctgtcggc gggaacggat agggtcgtct gaaaacgcat ctttattcaa atgaatgccc
    17221 acttcgcctt cactgcgccg ccgaacgact gaataatcgg ctccagcagc gcgtatttcg
    17281 ttttcaaggc agccttgttg accaccaggt agctggaaat gtcgacgatg tgttcgactg
    17341 cctccaagcc gtttgccttc aaggtgtttc ccgtcgaaac caagtccaca atcgcatcgc
    17401 tcaagccgac cagcggcgca agttccatcg agccgtacag tttgataatg tccacatgga
    17461 cacccttgcc ggcaaaatga gatgccgcga tttcaggata cttcgtggca atcttcagac
    17521 ggcatccggg ttgcgaagcg gcttcgtaat caaacccttt acgcacagca accatcatgc
    17581 ggcacttggc aatctccaaa tccaaaggtc ggtaaagccc cgtgccgccg tgttcgatca
    17641 gcacgtcttt gcccgcaatg ccgaagtccg ccgcgccgta gcggacataa gtcggcacat
    17701 cggttgcgcg gacaatgaca aggcggatgt tttcatggtt cgtcccgata atcagcttgc
    17761 gcgatttttc gggctcttca gtcggagcaa tgccggcagc ggcaagcagc ggcagcgtct
    17821 cctcaaaaat gcgccctttg gataaggcga tggtcaaagc attatcctgc atggtgtgga
    17881 tgtcctttgt cggttgaaac aataatgccg tctgaacagg caccggcggt gcagacggca
    17941 tacggtttta tcagagcagg gtccttacat cggcagcaat cgtttccggc tcgcttccgt
    18001 aaggcgagaa aatggcaacc tcaccgtttt tatcgataag atacgcaccg gaagagtggt
    18061 cgaccaaata gttttcgctg tcgtcttttt gattgatttt ggcagaaacc acgcggtatt
    18121 gctgcttgat gaccggcagg ttttgaccgc ccgttgccgt cagaccgata aagtccggat
    18181 tgaactgttt ggcatacttg ccgatgattt caggcgtgtc gcgttccgga tcgatgctga
    18241 cgaacaccac tttcacgtcc ttagcctgcc cgcccaactg cttcaaagtg tcgctgtacg
    18301 tcaaaagctc tgtcgggcag acatcgggac agtgcgtaaa gccgaaagac agaatcacga
    18361 ccttgccttt caaatcgctc aggctgaaag gcttgccttc gccgtcggtc agcgtgaaat
    18421 cgccgccgat gtcttcctta cgcatatccg taccgcgcgt ttgcggcttt gccgcatttt
    18481 ccgcagccgg cgcggatgca cttgaagaag cggcttgcgc cgcactgttg tcttgaggtt
    18541 tgcaggcggc aagcgcggca agtacgaaaa cgcccggcaa aaaggaacgc ggtacggaaa
    18601 acatgacaat ctcccaagaa taaatagaaa aacctgattc gtacggaatt tgaatatttc
    18661 catcttaacg cgtttggcag atttaatgta gtattttttt acgttgtccg tacggcagga
    18721 attgccgaat catacagaat ctttggagcg gaaagaacag taagattaaa ataataaaaa
    18781 ttctatttaa aatcaaaaac aaaataaaaa cagataaaat aatttcaaaa aaaggtttac
    18841 agaatctcaa aatccattat aatggcaatt tcttcaccag cccaggtggc gaaattggta
    18901 gacgc
//
LOCUS       DAXFWM010000045        17872 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000045 DAXFWM010000000
VERSION     DAXFWM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17872
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.045"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="ACFI8B_001415"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="ACFI8B_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC3834614.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(670..783)
                     /locus_tag="ACFI8B_001416"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="ACFI8B_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="ACFI8B_001417"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="ACFI8B_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC3834615.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="ACFI8B_001418"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="ACFI8B_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC3834616.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="ACFI8B_001419"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="ACFI8B_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC3834617.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="ACFI8B_001420"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="ACFI8B_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC3834618.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFI8B_001421"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="ACFI8B_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC3834619.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="ACFI8B_001422"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="ACFI8B_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC3834620.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFI8B_001423"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="ACFI8B_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC3834621.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="ACFI8B_001424"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="ACFI8B_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC3834622.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFI8B_001425"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="ACFI8B_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC3834623.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFI8B_001426"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="ACFI8B_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC3834624.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="ACFI8B_001427"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="ACFI8B_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC3834625.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="ACFI8B_001428"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="ACFI8B_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC3834626.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="ACFI8B_001429"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="ACFI8B_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC3834627.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFI8B_001430"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="ACFI8B_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC3834628.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFI8B_001431"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="ACFI8B_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC3834629.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="ACFI8B_001432"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="ACFI8B_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC3834630.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="ACFI8B_001433"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="ACFI8B_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC3834631.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFI8B_001434"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="ACFI8B_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC3834632.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFI8B_001435"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="ACFI8B_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC3834633.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="ACFI8B_001436"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="ACFI8B_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC3834634.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="ACFI8B_001437"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="ACFI8B_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC3834635.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGAFYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="ACFI8B_001438"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="ACFI8B_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC3834636.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFI8B_001439"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="ACFI8B_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC3834637.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFI8B_001440"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="ACFI8B_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC3834638.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFI8B_001441"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="ACFI8B_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC3834639.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFI8B_001442"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="ACFI8B_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC3834640.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFI8B_001443"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="ACFI8B_001443"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC3834641.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFI8B_001444"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="ACFI8B_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC3834642.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="ACFI8B_001445"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="ACFI8B_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC3834643.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="ACFI8B_001446"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="ACFI8B_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC3834644.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENRKMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="ACFI8B_001447"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="ACFI8B_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC3834645.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFI8B_001448"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="ACFI8B_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC3834646.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4955 a         3380 c         4409 g         5128 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtcaagc ttggtacatt tttgccaagt ttaactttgt ctgaaagaca ggccaatcgc
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa
     1141 gttggtatga cccgcgtgtt cgacgagcag ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagata ctgacggcta taccgccgtt
     1261 caagttacct ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt cggtcaactg
     1441 gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg gcacgatcaa acgtcataac
     1501 ttcggtgccc aacgtacttc ccacggtaac tcccgttctc accgcgttcc aggctctatc
     1561 ggtatggcgc aagacccggg tcgcgtgttc cccggtaagc gcatggccgg ccaatacggc
     1621 aacaccaaag caactgttca aaaattggaa gttgtccgtg ttgatgcaga acgccaactg
     1681 ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcccagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcaccagc
     1861 tggtaaatgc ctacttggca aacgcccgct ctggtaaccg tgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgc gcccgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc cggtatggcg actatcctgt
     2101 cccaattggc gcgtgacgag cgtttgtttg tgattgaggc gttgactgcc gaaactccca
     2161 aaaccaaagt ttttgccgaa caagtaaaaa atttggctct ggagcaagtg ctgtttgtaa
     2221 ccaaacggct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtattggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta taaaaaagta atcatcacta
     2341 aagatgcggt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 aatcttggcg cctgtcgttt ctgaaaaaag caacgtattg gctgaaaaat gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg tcgagctgct
     2521 gttcggtgtt caagttgctt ctgtaactac cgttaccact aaaggcaaaa ctaagcgttt
     2581 tggtcgtatt ttgggtcgcc gcagcgatgt taaaaaagct tatgtaagct tggctgacgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcacc ttatgcaccc ttgcttgaaa agaaaaattc tactgccggc
     2821 cgtaacaata atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggcatttctg ctaaagtaga gcgtatcgaa
     2941 tacgatccta accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgtcgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagtctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggtcat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gacggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagt tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtcttccag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtga cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgtgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg cgggtagtca aaatacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctttttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggt cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctacc ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatca tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggcg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggaatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg taaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctcgttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttccga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga taaaacgctt
    17581 gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca ggtttccgta
    17641 tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat
    17701 caggtaaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg gaatgacggc
    17761 atattttgac attgaataaa aaagactaaa aacagaaaac agaaaacaga aaacagaaaa
    17821 cagaaaacgg aaaacggaaa acggaaaacg gaaaacggaa aacggaaaac gg
//
LOCUS       DAXFWM010000046        17759 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000046 DAXFWM010000000
VERSION     DAXFWM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17759
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.046"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..115)
                     /locus_tag="ACFI8B_001449"
     CDS             complement(<1..115)
                     /locus_tag="ACFI8B_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC3834647.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYL"
     gene            complement(255..897)
                     /gene="pgmB"
                     /locus_tag="ACFI8B_001450"
                     /pseudo
     CDS             complement(255..897)
                     /gene="pgmB"
                     /locus_tag="ACFI8B_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            complement(910..3167)
                     /locus_tag="ACFI8B_001451"
                     /pseudo
     CDS             complement(910..3167)
                     /locus_tag="ACFI8B_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            3221..3364
                     /locus_tag="ACFI8B_001452"
                     /pseudo
     CDS             3221..3364
                     /locus_tag="ACFI8B_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3414..4375)
                     /locus_tag="ACFI8B_001453"
                     /pseudo
     CDS             complement(3414..4375)
                     /locus_tag="ACFI8B_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(4379..5733)
                     /locus_tag="ACFI8B_001454"
                     /pseudo
     CDS             complement(4379..5733)
                     /locus_tag="ACFI8B_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            5969..7645
                     /gene="ettA"
                     /locus_tag="ACFI8B_001455"
     CDS             5969..7645
                     /gene="ettA"
                     /locus_tag="ACFI8B_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC3834648.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDGLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            complement(7705..8190)
                     /locus_tag="ACFI8B_001456"
     CDS             complement(7705..8190)
                     /locus_tag="ACFI8B_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC3834649.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(8183..9139)
                     /gene="thiL"
                     /locus_tag="ACFI8B_001457"
     CDS             complement(8183..9139)
                     /gene="thiL"
                     /locus_tag="ACFI8B_001457"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC3834650.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            complement(9914..10624)
                     /gene="rmpM"
                     /locus_tag="ACFI8B_001458"
     CDS             complement(9914..10624)
                     /gene="rmpM"
                     /locus_tag="ACFI8B_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC3834651.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            10988..11938
                     /locus_tag="ACFI8B_001459"
     CDS             10988..11938
                     /locus_tag="ACFI8B_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC3834652.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(12051..12785)
                     /gene="fnr"
                     /locus_tag="ACFI8B_001460"
     CDS             complement(12051..12785)
                     /gene="fnr"
                     /locus_tag="ACFI8B_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC3834653.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            12920..14341
                     /gene="hemN"
                     /locus_tag="ACFI8B_001461"
     CDS             12920..14341
                     /gene="hemN"
                     /locus_tag="ACFI8B_001461"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC3834654.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIK
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPGDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(14379..14567)
                     /locus_tag="ACFI8B_001462"
     CDS             complement(14379..14567)
                     /locus_tag="ACFI8B_001462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834655.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(14581..16155)
                     /locus_tag="ACFI8B_001463"
     CDS             complement(14581..16155)
                     /locus_tag="ACFI8B_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC3834656.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            16480..17580
                     /locus_tag="ACFI8B_001464"
     CDS             16480..17580
                     /locus_tag="ACFI8B_001464"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC3834657.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRAVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         4339 a         4942 c         4625 g         3853 t
ORIGIN      
        1 tcaggtattg ttcgatcggc tgccaatcaa tcacctggtc caactccgat agcgggaagc
       61 ggccgatgtg tttggcagtc atggcttggg cggtttgccg gaagaaggtg ctcatgggaa
      121 atcccctaaa tgccttggtg ggaatttagg ggattttggg gatttttgca aaggtctcgg
      181 gttatcattc tgttctaaaa gcagaaaggc cgtctgaaac actgttcaga cggccttttt
      241 acttccttag aattttatct gcccgactgt tcccacacat tttgcaagta ggcgtaggtc
      301 agctcggcgg tgtcggacac cagcgcgatg tcgctgccca agtcttcggt tttgcccacg
      361 ccgatgggca aagcgccaga ggctttgatg gcggcgacgc cggcagcggc gtatgtccgc
      421 acccacgccc tcggcggcgg caaggaagat gtcgggggag ggtttggaat gcgcgacggc
      481 ggcagggtcg gcgacggtgt cgaagaagtg ggtcagcccc atgcgttcga gcaagaacgg
      541 gccgttttta ctggcggacg cgagggcgat ttttttgccg tttgccctca atgcttccag
      601 cagcggcaaa atgccggggt acacgtcttc gggtttgact gcctgaatca tctccaagta
      661 gttgtcgttt ttgcggcagg tcagttcggc gaactcggtt tcgccgacgg ttttgccgcc
      721 gtgcgcgagg atgcgtttga gcgaatcgtc gcgcgacacg cctttgagct gctcgttaaa
      781 cttgcggtcg atgccgatgc ccaattcttc ggcaagcttt ttccatgcga ggtagtggta
      841 ttcggcggta tcggtaatca cgccgtcgag atcgaagagc actgcggtaa aagtcatttt
      901 gcgccatcct tatttttcca atgcaacggt gtggctgccg ttgagcgtga tgtctttgcc
      961 gtacacctgc aattcgagcg gatcgccttt gagcagggtg aagacgacgt tttttgccga
     1021 cggcgacttt aatcagacgg ccgcggtagt tgatgtggaa ggcatagcct gtccacgcac
     1081 tcggcaggaa cggtgcgaag ctgagtttgc cgccccaagt tttcatttgg gcgaaacctt
     1141 ggacgatggc gagccacgag ccggtcatag aagtaatatg caggccgtct tcggtgtcgt
     1201 tgttgtagtc gtccaagtcc aggcgggcgg tgcgccggta catttccacg gctttttctt
     1261 ctttgcccag ttcggcagcg aggatggagt ggatacatgg agacaacgag ctttcatgca
     1321 cggtcatcgg ttcgtagaag tcgaagttgc ggcgtttttc gtccatgtcg aaacggtcgc
     1381 cgaagaagta gatgccttgc aatacgtccg cctgtttgat gaagggcgaa cgcaggattt
     1441 tgtcccacga ccatttctgg ttgagcggca aatcgtcggg cgaaagcgcg gatacggggc
     1501 ggatgtcttt gtcaaggaag ccgtcgtgct gcacgaatac gccgagttct tcgtcatgcg
     1561 gacggtacat attcgcgctg atgtccgccc atttttccaa ctcggcggcg ctcacgttcg
     1621 aacccggacg cgggtatttc gccaaggctt cgcgggtgta gcccaatacc catgcggcga
     1681 gggtgttggt ttaccagttg ttgttgatgt tgttttcgta ttcgttcgga ccggttacgc
     1741 cttgaatcat gtatttgccg ttgcgtttgg agaagtggac gcggtccgcc cggaagcggg
     1801 acacttcgac caagacttcc aagccttctt tggcaagata gcactcgtca ccggtgtagt
     1861 tggtgtagtt gtagatggcg taaggaatcg caccgttacg gtggatttcc tcgaaggtga
     1921 tttcccattc gttgtggcac tcgatgcccg taaacgttac catcggatag agtgcgcccg
     1981 ccaagccctg ttcgcgtgcg ttatgctgca cctgcggcaa tttggttgcg gcggtattgc
     2041 agcaggttgc gggtaacttc gggttccgcc agtgcaaggt agagcggcac ggcgtaggct
     2101 tcggtgtccc aataggtcgc gccgccgtat ttttcgccgg taaagccttt ggggccgatg
     2161 ttcagacgcg cgtcttcgcc gtagtaggtg gagaacagtt ggaacaggtt gaagcggatg
     2221 ccctgctgtg cttcgtcgct gccttcgatg accacgtcgg cgatttccca acgatgctgc
     2281 cagcctgctt tgtgcgcatc cagcaaggtt tcaaacgcaa cacctgcgat tttttcagat
     2341 aaggcgcggc cggcggcttt cactgcttcc aagccctgat aatcgcggct ggtggtaaca
     2401 atcacgcgtt tttcaaaggt ttcgggcgtg ccgccgactt cggcttcaaa agaattagag
     2461 acctgccaat cggtttggct gccgccgaag gctttgaagc tgccggcgaa ggtttgcccg
     2521 gcgttgacga taaattgttc cacaccgaaa gggttggcga cggtttgggc ggcaatgtag
     2581 gagcggtcgt ctgaaacgcc tttgtccaat acctgccaga atttttcttc gtagttggag
     2641 tcttcgtttt tcacgtcggc atcgatgatg gaatcgatgc ggacttggtg ggttttgccg
     2701 tcaacggaca cggcttccca gcgggtgaga gccagctctt tttgcgcgac cgacaggaat
     2761 ttgcacacat cgaaacgcac accgaatacg gtgaacgagc ggcgcaacac gccgtgttgc
     2821 atatccagtt cgacggagaa gccggcaacg tcgtttttcg ccaagtccac ctcttgcccg
     2881 tcgacaaaga ttttgacttt gctgaaattg agcgcgttga tggctttgcc gaaatatttg
     2941 ggatagccgt ttttccacca gccgacgcgg gttttgtcgg gaaaccacac gccggcgatg
     3001 taggtgccca agtggctgtc ggcggagtag gtttcctcaa agctgccgcg catacccata
     3061 tagccgttgc ccaagctggt caggctctct tgcagccgtt tgtgttcttt ttccagtttt
     3121 gccgaacgca gcgtccaagg gctgatttcc ataattcttg tgtacatcgt aaagctcctg
     3181 tttggattga tttgagggaa tggtgaaatc ttatagtgga ttaacaaaaa tcaggacaag
     3241 gcgacgaagc cgcagacagt acagatagta cggcaaggcg tggattaaca aaaatcagga
     3301 caaggcgacg aagccgcaga cagtacagat agtacggcaa ggcgaggcaa cgccgtactg
     3361 gtttttgtta atccactata aaaaggtcgt ctgaaaccgg tgttaggaag ctcctaagaa
     3421 agggattcga tgccgtaagc aatcgtcgcc tccctagtat cacccttgtt cagacggata
     3481 ttgccgaatt cgggccaatg caggctgtcg ggcagcgtct gcgcctcggt cgccagcgcg
     3541 tcgtaaacgc ccgcatcgtg ccgcgcgaaa tcctgcgggg cggcggtaaa gatgaccaag
     3601 ccgttgcggt cgctgtatat gctgatacga cggcggcgtc cggcttgcaa cactgcggcg
     3661 ggacggccta tatcggacgg cacgcggtaa gcgtcgtcaa aaccggcccg tttcgcggcg
     3721 cagggcggca acggcggcat ccggcggctt gggccggctg aaatcaaata cttcgaggcc
     3781 gtctgaaacc gttaagacgg gcagtttttc ggcatcggcc ggaatatgtc cgccctgcgg
     3841 aatatgcaga accgcatcgt gcaggcccgc gtccagccgc cagtaaatgt gcagcgtcgg
     3901 gtcgaacacc gtgtcgccga gcgcggtggc gcgataggta acggtaagcc ggccgtcctc
     3961 gtccaagcgg taggaaatat ccaaatcgtt gggataaccg tcggccgtct gccgccaccg
     4021 cgttgaaacg ggtaacggcc agcccgtgcg aaccgccgtg cagcgcgttc ctgccttcgt
     4081 tggcctccac gcggtaagtc ctaccgttga tgtcgaacgc cgcaccgcgg atgcgtccgg
     4141 ccacgcgccc tatctgcttg ttaatctgaa acggattgtc cgcataggaa gccgcatcgt
     4201 cgaacgacac cacggggttt tcgcgcacgc cgtctgccaa aacggaaaat tcctgcacaa
     4261 tcccgcccaa gtccagcacg aagacgcacg taccacgccg gttggacagc acatagccgg
     4321 ttacggcccg cccgtcgatc aggccgaaat cgcgggtagc gggggtatcg ctcatcgctc
     4381 aaaccccgcc gtggatctct ttaatcagac agactgagaa ggctcccagc agcaagactg
     4441 cgcctgcaac caagaacatg gttgcctgat ggccgcccag catcgggaaa agcacgaaac
     4501 tcaacagcga agcgacgatt tgcggcatac agacagagcc gttaaacagg cccaaataag
     4561 tatccatgtg tttgcccgac aaagcgttgg ccacaatcgt cagcggataa gtgataatgc
     4621 ccgcccaagc gatgccgatt aagatataag acaggatgag tgcgtattga ttgtagatga
     4681 agaagataga gaagaaaccg agcgcgccca aagccaaaca gccgaaataa cccgccttat
     4741 ggtatttatt cggtactttt gccagaataa acgaacaaat caccgccgca accgactaca
     4801 ccgccgccaa aacgccgtac cggttgcccg cctcctgatg gcctacggaa gacgcatcgg
     4861 tagtgtgcca gacgttttct gcaatcgcgc ctgccgagta agtccacata taccggaagg
     4921 cgaaccagca gaaaaactgt accggagtaa ccgtccaaaa cactttaggc gcggttttta
     4981 agagttcgaa ccagttggct ttttcctgat tcgcggcgac atcgatgccg tggtaacggg
     5041 cgtaggtttc cgggtcgtat tctttgactt tggagattgt gaacgcactg gtaataatca
     5101 gtaacgccgc acccacatag aatgctacga ccacggtttg tggcacaacg cctttctcgg
     5161 cagtgttcgc caaaccgata tacgcgaaca caaacggcag aatcgctgcc acaaccgcgt
     5221 ccgtattcgc taagaaactt tgaatcccgt aggcgtagct tttctgctcc tcgttgacca
     5281 tatcgccgac catcatctta aacggctgca tcgccatatt cgacgacacg tccaacagcg
     5341 caatcatcag cgcgccgaac gacaaggccg ccagcgacgc atagccgaaa ccgaagctgc
     5401 ccgagttcgg catcaaaatc atcacgatga ccgcaatcag cgtgccgtaa agcagatacg
     5461 gcaggcggcg gccgcccaag cgcggcttcc aagtgcggtc tgagtagtag ccactatcgg
     5521 ctgaaccagc atccccgcca gcggcggcag gatgaaaaac cagcccaaat tgtgcgggtc
     5581 tgcgcctagc gtttgaaaaa tgcggctcat ctgcgagctt tgcagggtaa aggccgtctg
     5641 aacgccgaga tagccgaagc tcaacatcca aatcgtgctt tttgccggcg cgggcaaacc
     5701 ttgttttgct gtttgaggcg tatattccga cataaagtaa atcctttttt ttgatttgaa
     5761 aagtatttgc tttggaaaat ccgaaatggt tgccggcgcg gcgatcccct atcattatta
     5821 tttttttgtc tgtataattt caaagggata ggcggatttt atgaatcctg cccgattttg
     5881 gcaataccgg ttcgcggata aactggctta aatcaaatta tcggttaaaa tggccgtctg
     5941 aaatttgttt gaccgaatcg agaaaactat gtcccaacaa tacgtctatt ctatgctgcg
     6001 cgtgagcaag gttgtgccgc cgcagaaaac catcattaaa gatatttccc tttctttctt
     6061 ccccggcgcg aaaatcggcc tcttgggctt gaacggcacg ggcaagtcca ccgtgctgcg
     6121 gattatggcg ggcgtggata aagaatttga gggcgaagcc gtgccgatgg gcggcatcaa
     6181 aatcggctat ctgccgcaag agccggagct tgatccggaa aaaaccgtgc gcgaggaagt
     6241 ggaaagcggt ttgggcgaag tggctgccgc acagaaacgt ttggaagaag tgtatgccga
     6301 gtacgccaat cccgatgcgg attttgacgc gctggcggaa gaacaaggcc gcttggaagc
     6361 gattatcgcg gcgggttcgt caactggcgg cggtgcggaa cacgaattgg aaatcgctgc
     6421 cgacgcgctg cgcctgcctg attgggatgc caaaatcggc aatctttcgg gcggtgaaaa
     6481 acgccgcgtc gctttatgca aacttttgtt gagcaagccc gatatgctgc tcttggacga
     6541 gccgaccaac cacttggatg cggaatcggt ggaatggctg gagcaattcc tcgtgcgctt
     6601 ccccggcaca gtggtcgcgg taacgcacga ccgctacttc ctcgacaacg ccgccgaatg
     6661 gattttggaa ctcgaccgcg gacacggcat tccgtggaaa ggcaattact cgtcttggct
     6721 ggagcagaaa gaaaaacgct tggaaaacga ggcgaaatcc gaagccgcgc gcgtgaaggc
     6781 gatgaagcag gaattggaat gggtgcgcca aaatgccaaa ggccgccaag ccaagcccaa
     6841 agcgcgtttg gcgcgttttg aagaaatgag caactacgaa taccaaaaac gcaacgaaac
     6901 tcaggaaatc tttatccctg ttgccgagcg tttgggtaac gaagtgattg aatttgtgaa
     6961 tgtttccaaa tcgttcggcg ataaagtgct gattgacggt ttgagcttca aagtgccggc
     7021 gggcgcgatt gtcggcatca tcggcccgaa cggcgcgggt aaatcgacgc tgttcaaaat
     7081 gattgcgggc aaagagcagc ccgattcggg cgaagtgaaa atcgggcaaa ccgtgaaaat
     7141 gagcttgatt gaccaaagcc gcgaaggttt gcaaaacgac aaaaccgtgt tcgacaacat
     7201 tgccgaaggt cgcgatattt tgcaggtcgg acagtttgaa atccccgccc gccaatattt
     7261 gggacgcttc aactttaaag gcagcgacca aagcaaaatc gcaaggcagc tttccggcgg
     7321 cgaacgcggc cgtctgcact tggcaaaaac cttgttgggc ggcggcaatg tgttgctgct
     7381 ggacgaaccg tccaacgatc tcgacgtgga aaccctgcgc gcgttggaag acgcattgtt
     7441 ggaatttgcc ggcagcgtga tggtgatttc gcacgaccgc tggtttctcg accgcatagc
     7501 cacgcatatc ttggcgtgtg aaggcgactc caaatgggtg ttcttcgacg gcaactatca
     7561 agaatacgaa gccgacaaga aacgccgact cggcgaagaa ggcgcgaaac cgaaacgcat
     7621 caaatacaaa ccggtaacgc gttaacctcc gaaacaatgc cgtctgaaag gctttcagac
     7681 ggcattttta caaggcagcc ccgtttaaaa cagcattgca atcctcaaga caatcaaggt
     7741 catcaccgca gccgccatat cgtccgccat aatgcccaaa ccgccgtgca gattcttgtc
     7801 aaaccaaccg acggggggcg gtttgagcgc atcaaacaga cggaacagaa caaatgccgc
     7861 cagccaccac gtccacctga acggcacaaa cgccagcaca aacagcatgg cgacaatctc
     7921 gtcccaaaca atcccaccgt ggtcgctgac acccgtttca cgttccgcac acgcacaaat
     7981 acgtatgccc cacataaaca gcacgataca cacaaaagcc agcagcagcc cgtctatgcc
     8041 gagcaaaatc agcacaaacg ccaaaggcag tgccgccaaa gtgccgaatg cgcccggtgc
     8101 aaacggagcc agcccgctgc cgaaaccgaa agccaaaaaa cacaacggcc gtttcaacag
     8161 ccacgcaaag tcaggtttaa aatcagccaa aatgatcgaa tcctaaagaa tgtagttcca
     8221 attccctgcc gccggcatct aaaaccttca gacggcatcc tccgttgatt ttgccgatgc
     8281 acgttaccgg cacgccgcac cgttccgccg catcaagcac gcggctgcgg caactttccg
     8341 gagcggtaaa aaccagctcg taatcgtcgc cgcccgccaa agtataagac agccattgcg
     8401 ctcggggcaa aatatctttc aatacagata aagatggcag cgaatcggcc caaatttccg
     8461 cccccacgcc ggaagcggtc aggatatgcc ccaaatcttg cgcaaggccg tctgaaacat
     8521 cctgtgccgc ccgggcaaac ggcaacagcg caagccccag cccaaccctt ggctcgggac
     8581 ggagcagctt ttgttcgcat tcggcgaaca catcatccgg caacacacac cgtttcagac
     8641 ggcagttcaa agccgccgcc gccataccga cacgccccga cacccaaata tcgtcgcccg
     8701 caaccgccgc atcacgccgc aacgccctac ccttcggcaa ttcgccgata atggttacat
     8761 tgaacgccat atcgcccttg gtcgtatcgc cgccgattaa cgttacgcca aacttttttg
     8821 ccaaaccgaa aaagctgccg caaaaccgtt ccagccatac ttcatccaat tcgggcaacg
     8881 ccgcgctcag cagcacccaa cgcggtatcg cacccatcgc cgccatatct gaaatattga
     8941 cggccaaaac cttccaagcc aagccttcag gtttgacatc tgcaaaaaaa tgcctgtcct
     9001 tcaaaagcat atccgcactg aaacacaaat cgaagccttc gcgcgggcgg acaatcgccg
     9061 catcgtcgcc tatgcccaat acgacatcct tatccgtacc tgtttgcaaa taccgtttga
     9121 tgaagtcgaa ttctttcata tcccaaaata tccggcgtgc gaatccaatt taaatcatag
     9181 caaagcagca aaggtcgtac aaacaataaa gcggctcatt cagtgtttca gcgtatttca
     9241 cttttagctt tagccatacc gtacccgtat gcaccgacca ccctgtttca acacacaaac
     9301 ggcatatcaa agcaccgcac tgcacgccgc cccgaatgca aagcggcaac ttccgccacc
     9361 gattaggctc aatcgctgca actggcgggg tttcaaccga aaagggatga tgataaaacc
     9421 atatccggca tacagtgccg cacacatcgg gcacgattcc atccatttcc caatgagggg
     9481 gaaaggcgtg aatttgtatg aattatccct ttcggggctt gatacagaaa ggattccctt
     9541 gctatgggca aacccacatc ctaccgcctt aaaatcagag tttcaaccga tatagtggat
     9601 taaatttaaa ccggtacggc gttgcctcgc cttgccgtac tattgtactg tctgcggctt
     9661 cgtcgccttg tcctgatttt tgttaatcca ctataaagcg cggcaatggg acacaaacat
     9721 gaatctgaat ccgaatccga acctgcaccc cgattctgac gatagccgtt tcagaaaacc
     9781 acccaaaaaa acaaaccccg caaaagcggg gtttgtttca catcatcgat accggccgac
     9841 gtttcacgaa tattgcaaca gtacccggta caaaatccgc taacctcatg catcggcaag
     9901 atattaccta gccttagtgt tggtgatgat tgcgtgccgg cacaacctga cgggttacga
     9961 tgctgcggat tttcacatct acgcggcggt caggttcgat acatgcaatc agagcctcac
    10021 gttttttggc tttagaggct ttcgcaccca gtttggcaac ttcggcttga caaacttgag
    10081 tcatttgcgc ttgagattcg cccaagccga cagcagaaat tctagaagca ggtacgccgt
    10141 tgctgaccag gttgtttgcc actacgtatg cgcggcgttc ggacagagcc tgattgtatt
    10201 tttcagaacc cataaagtcg gtatggcctt cgacgcggac agattggaca ttggttcgac
    10261 tcaggcgttg cgccaatact ttcaggttgt cttgagcttc ggcgcgcaat gaatccttat
    10321 cgaaaccgaa cagggttttg gcagacaggg aaatggtttc atcaacatat tgaggagcct
    10381 gctccacaac ggcgacaggc gcgggttcgg gctcggggac ggcaaccgca tcgccgcatt
    10441 ctacgcgacc ttggcttgct ttatcaaagt aggcgttttt ccagcattct ccatagttgt
    10501 tgcgtacgat ttcgttcgac tggccgctta cggtgtaacc ctgaacggac gcctcgcccg
    10561 caacagcagt gccggaagcg agcaatgcaa cgaataatgc gcttaatttc agctgtttgg
    10621 tcattttatt ccctcattag atttgtacag cagatacaga agcaactgct gaattgccaa
    10681 catacaggcc gctgtagaaa catggcggca aatcggatat ttcagtctcc actataaaga
    10741 aacagggggc ggactgtcaa tactcaacgc ctgaaaacaa ccgtaaaaca gcctgtttcc
    10801 tgatagtcta tcatgccgaa aaatcaattc cgtgtcatta ctttaggggg attttttcca
    10861 tatcgcacga ttgccgttgc acaaaaacaa caaacacaac ggcaaagccc cataccgtac
    10921 cggcaagaaa atatgataaa ttataaacaa tgttcagcca cccgacacag acccacaccg
    10981 acccgccatg aaattacaac aattgaaata cgccttagaa gtttaccggc acaacctgaa
    11041 cgtttccgaa gcggccgaag ccttattcac atcacaaccc ggcatctcca aacaaatcaa
    11101 attgctggaa gaagaaatcg gcattcagat ttttatccgc agcggcaagc gcgtggtttc
    11161 ggtctcgcag ccgggcaagg tggttttgga tattgcggaa cgtattttgc gcgatgtaca
    11221 gaacattaaa aatatcggca gcgagtttac cggacaggac agcggttcgc tgacggttgc
    11281 cacgacgcat acgcaggcgc gctatgccct accgttgatt gttgccgatt ttgtgaaacg
    11341 ctatccgaaa gtcaatctga ccatcaaaca gggaagccct gccgccatcg cccaaatggt
    11401 tacttcggga gaatctgatt tggcgattgt tacggaacgg atagacgacc accccgagtt
    11461 gggaaggctt tcctgctacg actggacaca cgcggtgatt gtgccgaacg accatccgct
    11521 gctcgaatgc aggaatcccc tccgtattga agatttggcg aggtttccgc tgattactta
    11581 tgaatttgca ttcaatgcgg gcagcagcat cgcgcgggca ttcgccaaag cccgtttgga
    11641 acggccggat gtcgcattgg cggcggcgga tacggatgta ttgaagactt atgtgcgctt
    11701 gggtttgggc gtgggactga tggcgaaaat ggcatacaac ccggatacgg acggcgattt
    11761 gcagcttgtg gatgcggcac acctgttcga gccgtcgccg acgtggatag ctttgcgcag
    11821 cgatacttat ttgcgcggat atgcctacga ctttatccaa gcgtttgcgc cgcacctgac
    11881 acgcgagaag gtggatagga ttctgtacac gcccatcagc gaggattttt cgatttaggc
    11941 ggctgccggt tttcaggcgg cactttgcgg cagatacaac aaacagggca gatgttttcg
    12001 tctgccctgt gtttattgag aatgccgtct gaaatgttcg tacaggttaa tcaaatggcg
    12061 tgcgaacaac cggataccat ttttttcagc acctgcagat tgagaatctt gatgtgtttg
    12121 tgttcgacgg aaatcaatcc ttcctgatgg aacttggata atgtgcggct gacggtttca
    12181 agtttcagcc cgagataact gccgatttct tcgcgggaca ttcttaagat gaagtcgttg
    12241 gcggcgaaac ctcgggaata aaggcgttgg gaaaggttca acaggaaggc ggcaatccgc
    12301 tcttcggcgc gcatattgcc caacaacagc ataacacctt gatcgcgcac aatttcgcgg
    12361 cttatcatgc ggaaaaagtg tgtacgcagg ctggggatat tctgaccgag ctcttcgata
    12421 tgggtaaacg gcaactcgca tacctcgctg ttttccaagg cgaccgcgtc gcaactgtgc
    12481 acataggaac agatgccgtc catgccgatg agttcgcccg acataaagaa acccgttacc
    12541 tgatcgcggc cgtcctgact ggcgacggtt gttttgaaga agcccgaacg gatggcaaag
    12601 agcgaggtaa aggctccgcc ggcacggaac aggtattcgc cttttttcag gcggcggctt
    12661 tggcggatga cggcatcgag ttggctgaac tcgttgggca gcagcccgac aggcaggcag
    12721 agttcccgca aagaacagga agaacacagc gttttcatct gatgtgtagt attatgcgaa
    12781 gccataccgt accttttcgt gtgttttgcc ccatcccgat tatgtgcttg ttgacacata
    12841 tcaagacagg tttatcatac tgtggcattc taccaaacta tccaaaattt gactaacatc
    12901 ataaaaccgc acacacacca tgaaaatcat tcagatacag aacaatcaca atgtaaacga
    12961 tgaccgcccc gagtttgacc gcgcgctgat tgccagcctg cccgccagcg gcccgcgcta
    13021 tacttcctac cctaccgccg accgtttcca tgacggtttc cgcgaaggcg aatatatcaa
    13081 agttttacat ttgcgcggta tgggcgcgtt aaacaaaccg ctttcccttt acattcacat
    13141 tccgttttgc aacaccatct gctactactg cggctgcaac aaaatcatca ccaaagacaa
    13201 aagccgcgcc gatacctaca tcaaatatct tgaaaaagaa atggaactgc tcgctccaca
    13261 tctgaacgga cggcaccagc ttgcccaact gcacttcggc ggcggcacgc cgaccttttt
    13321 gagcgacgaa cagatcgaac gtgtcttccg catgatccgc aaacatttcg aactgatacc
    13381 gtccggcgaa tattccatcg aaatcgaccc gcgcaaagtc agccgcgaca ccgtcctcat
    13441 gctcggcaga ctcggcttca accgcatgag cgtcggcatt caggatttcg accccaaagt
    13501 gcaggcggca gtcaaccgca tccaaagcta cgaagaaacc aaagaagtca tcgatgccgc
    13561 ccgtgaggca gggttcaaat ccgtcagcgt cgatttgatt tacggcttgc cgcatcagac
    13621 ttccgaaagc atcaaaacca ccatagatac cgttttgtcg ctcgatcccg accgcctcgc
    13681 cctttaccac tacgcccacc tgccgcacgt gttcaaaccg caacgccgca tcgataccgc
    13741 cgccgtcccc gacagcgaag agaagctcga tatgctgcaa tactgcgtcc aaaccctgac
    13801 agagcgcggc tacgtcttca tcggcatgga ccatttcgcc aaacctggcg acgaactctc
    13861 catcgccctc aaagaaggct tcctccaacg caacttccaa ggctattcga cctacgcgga
    13921 ttgcgatttg gtcgccatcg gcgtgtcgtc catcggcaaa atcggcagca cctattccca
    13981 aaacgaacgc gacatcgatg cctactatgc cgccatcgac gaaggcagac tgcccatcat
    14041 gcgcggctac cagctcaatc aggacgacat cctgcgccgc aacatcattc aggatttaat
    14101 gtgccgcttc gcgctcgact acaggattta cgaaagtatg ttcggcatcc cgttcgaccg
    14161 ctacttcaaa gacgaactgg cggatttgga aaaactcgcc ggtttgggat tggtgcgcct
    14221 gaacagccac gggctgaccg ttacaccgaa aggacgcttc ctcatccgca acatcgccat
    14281 ggtcttcgac taccacctgc gccacaaaga aaccaaagcg aaatattcgc aaacggtgtg
    14341 attgcggcta acgtacaaaa gccgtctgaa aggctttttc agacggcatt ttgctgccga
    14401 ggataagtgt tttcaagaac aggcggcggc atatcataac ctttgtgtcc gaccgttccg
    14461 aaaccaagat accaaacagc atatcccgac agaacagatt gtgcataagg cacaagcccg
    14521 cacattccat caacaaaatg ccgtctgaac acgggttcag acggcatcag tattttacaa
    14581 tcagaacact gcctgcaaaa tcaaatagct ggccatcgac aatacagcgg cggcaggcag
    14641 ggtaatgacc cacgccaaac cgatgggttt catcagtttc cagttggcat tgcggttgac
    14701 cagaccgata ccgagtaccg cgccgaccaa gatatgcgta ctggacacgg gcagccccat
    14761 cagcgacgcg cccatcacga cggatgcggc ggacagttcg gcggtaaaac ccgaagcagg
    14821 atgcatttcc gccaaactcg taccgacggt tttaatcacc tctttaccga caaaccacaa
    14881 accgacaatc agcgcgatgc cgaaagtcag catcgcaatc ggagggacgg cgctttgcgc
    14941 ggcaacgctg ttcgtccgca aaacatccat aatcgcggca aacgggccga tggcgttggc
    15001 gatatcgttc gcaccgtggc tgaatgcgaa gccgcaggcg gtaaagacct gcatccatga
    15061 aaacatctga aaggtcgatt tgcccaagtc tttacgcttg aggcttttgg caaacacaaa
    15121 cgtccccatc cacaccgccg cgcctatcat aaagatggtc aggaagctgt tgacgttgct
    15181 catccccaaa tgcaggtttt tcaagccctt gaaaatcagc atagcggaaa tcatcatcgt
    15241 gccgaacgaa gcgataaagg gaatccaaga atgcagcgcc ttgtaggaat cgacattgtt
    15301 tttacggttg tcgaacgcat aaagaccgcg gtaatactcc gattgcagct cttgcggatc
    15361 gaattcgggt tcgtcgtaaa tttgcgcgtc gtgcgccatt ttggtggcgt actcgacttt
    15421 ttcggcttcg gacaaaccct cgaaaaacag gcggtgccgt tctttatagg cctttttttc
    15481 ctgcttgatg cccttgagcg ttccttccgc ccaagcgttg taatctaaga cgtttttctt
    15541 gacgcgcgaa aacaggaaat aggacaccgc gccgcccaac acgggcgaca atacccaaga
    15601 catcccgatt tcgcccagct tgccccaccg tatcaacgcc accccgtccg cattatgcgt
    15661 aaacgccata cacaacgcac tgccgacgat gccgccgata atcgcgtgcg tcgtcgaaac
    15721 cggcaggcct ttgcgggacg cgaacagcag ccacaacgcc gccgccaaaa gcgcggacat
    15781 cataataaac acaaactgta tgggttcgaa atcaacaccc ttcaaatcga cgatgccttt
    15841 gcgtatggta tcggttacct cgccgcccgc gatgaccgcg ccgctgacct caaataccgc
    15901 cgcaatcagc aaagcctgcg ggatggtcag cgtgcctgca ccgacgctgg tgccgaaaga
    15961 attggcaaca tcgttgccgc cgatgttgaa cgccataaac acaccgaaca tcgtggcaat
    16021 aatgaataag gcggcgtaat tgtaatgggt ataacccaag ccccaataaa taaaatagcc
    16081 gactgtacct accagcatgg cggcaaagac ggcgttgatg gttttaacat ttgcgctcat
    16141 ttggatttga gccatggcaa tcttccttta atgtggtgga aatcatgagt gttgccgaca
    16201 ctgtattgct gcctccggtc gaactgccgt ttcgggatat ggcagacgca tccttccttc
    16261 tgaaccgcat ccgaataccc ccaaacctga ccgaatgcaa aactgctgcc ggaaacggtt
    16321 tgtatgacac ttctactact ggatgttgcg ggcgcattat aatatttttc cacccgtctt
    16381 aaaacattta tttacacttt atttacactg cggcggcaaa tcggtatacg agcgtcaata
    16441 cacgttaaaa tggcgttttg caccagtttg ggagtgatga tggaaacaca gctttacatc
    16501 ggcattatgt cgggaaccag tatggacggg gcggatgccg tgctggtacg gatggacggc
    16561 ggcaaatggc tgggcgcgga agggcacgcc tttaccccct accctgaccg gttgcgccgc
    16621 aaattgctgg atttgcagga cacaggcaca gacgaactgc accgcagcag gatgttgtcg
    16681 caagaactca gccgcctgta cgcgcaaacc gccgccgaac tgctgtgcag tcaaaacctc
    16741 gctccgtgcg acattaccgc cctcggctgc cacgggcaaa ccgtccgaca cgcgccggaa
    16801 cacggttaca gcatacagct tgccgatttg ccgctgctgg cggaactgac gcggattttt
    16861 accgtcggcg acttccgcag ccgcgacctt gccgccggcg gacaaggtgc gccgctcgtc
    16921 cccgcctttc acgaagccct gttccgcgat gacagggaaa cacgcgcggt actgaacatc
    16981 ggcgggattg ccaacatcag cgtactcccc cccggcgcac ccgccttcgg cttcgacaca
    17041 gggccgggca atatgctgat ggacgcgtgg acgcaggcac actggcagct gccttacgac
    17101 aaaaacggtg caaaggcggc acaaggcaac atattgccgc aactgctcgg caggctgctc
    17161 gcccacccgt atttctcaca accccacccc aaaagcacgg ggcgcgaact gtttgcccta
    17221 aattggctcg aaacctacct tgacggcggc gaaaaccgat acgacgtatt gcggacgctt
    17281 tcccgattca ccgcgcaaac cgtttgcgac gccgtctcac acgcagcggc agatgcccgt
    17341 caaatgtaca tttgcggcgg cggcatccgc aatcctgttt taatggcgga tttggcagaa
    17401 tgtttcggca cacgcgtttc cctgcacagc accgccgaac tgaacctcga tcctcaatgg
    17461 gtggaggcag ccgcatttgc gtggttggcg gcgtgttgga ttaaccgcat tcccggtagt
    17521 ccgcacaaag cgaccggcgc atccaaaccg tgtattctgg gcgcgggata ttattattga
    17581 gcgcaaatac cgtctgaaag ccatttccgc cgttcagacg gcatttccac ccctttcgtt
    17641 tacaaaccct taaaatccct ttatactcaa acccgttcaa aaaaaaacaa acccgccatg
    17701 aaaaccctgc tcctcctcat ccccctcgtc ctaaccgcct gcggcacact gaccggcat
//
LOCUS       DAXFWM010000047        17745 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000047 DAXFWM010000000
VERSION     DAXFWM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17745
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.047"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(104..442)
                     /locus_tag="ACFI8B_001465"
     CDS             complement(104..442)
                     /locus_tag="ACFI8B_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC3834658.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            594..4550
                     /gene="purL"
                     /locus_tag="ACFI8B_001466"
     CDS             594..4550
                     /gene="purL"
                     /locus_tag="ACFI8B_001466"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC3834659.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYILSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            complement(4621..5832)
                     /locus_tag="ACFI8B_001467"
     CDS             complement(4621..5832)
                     /locus_tag="ACFI8B_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFC3834660.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5906..6217)
                     /locus_tag="ACFI8B_001468"
     CDS             complement(5906..6217)
                     /locus_tag="ACFI8B_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC3834661.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            6449..7201
                     /gene="gloB"
                     /locus_tag="ACFI8B_001469"
     CDS             6449..7201
                     /gene="gloB"
                     /locus_tag="ACFI8B_001469"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC3834662.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDNFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAEHTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            7435..8889
                     /gene="mgtE"
                     /locus_tag="ACFI8B_001470"
     CDS             7435..8889
                     /gene="mgtE"
                     /locus_tag="ACFI8B_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC3834663.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(8975..9883)
                     /gene="hslO"
                     /locus_tag="ACFI8B_001471"
     CDS             complement(8975..9883)
                     /gene="hslO"
                     /locus_tag="ACFI8B_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC3834664.1"
                     /translation="MNQTAINCADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            10128..10796
                     /locus_tag="ACFI8B_001472"
     CDS             10128..10796
                     /locus_tag="ACFI8B_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC3834665.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGSAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLL
                     GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGAPVARSELQPGDMV
                     FFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARRIKKNDPS
                     RFLN"
     gene            10815..11033
                     /locus_tag="ACFI8B_001473"
     CDS             10815..11033
                     /locus_tag="ACFI8B_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834666.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            11178..11522
                     /locus_tag="ACFI8B_001474"
     CDS             11178..11522
                     /locus_tag="ACFI8B_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC3834667.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            11522..12214
                     /locus_tag="ACFI8B_001475"
     CDS             11522..12214
                     /locus_tag="ACFI8B_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC3834668.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            12281..13501
                     /gene="argJ"
                     /locus_tag="ACFI8B_001476"
     CDS             12281..13501
                     /gene="argJ"
                     /locus_tag="ACFI8B_001476"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC3834669.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            13561..14970
                     /locus_tag="ACFI8B_001477"
     CDS             13561..14970
                     /locus_tag="ACFI8B_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC3834670.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIKELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLHRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            15158..16549
                     /gene="hrpA"
                     /locus_tag="ACFI8B_001478"
     CDS             15158..16549
                     /gene="hrpA"
                     /locus_tag="ACFI8B_001478"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC3834671.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEVV"
     gene            16617..>17745
                     /locus_tag="ACFI8B_001479"
     CDS             16617..>17745
                     /locus_tag="ACFI8B_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFC3834672.1"
                     /translation="MNIKEFMSNYTNHPVLFIGTGMSLRYLDNSYTWDDLLSKIAIDL
                     FGDDREYLNIKSRYCEDGRFQYEEIAEELQSKFDKVLENDPDGRFKEINDKFFENMRA
                     GNTLSRFKIYISTLLSQLNYKDNSNTELSELKKARKNVGSIITTNYDKLAQDIFEFNP
                     LIGNDILLSNPYGSVYKIHGCVDDPSKIIITKKDYEKFKEKYELIRAQLLSLFIHNPI
                     IFLGYNVGDENIKEILKTIFTYVEQNSPSANKIRRNFLLVEYEPESNNEDIVEHDIDI
                     TGFSTIRINKIKTDNFSQIYKALAELTLPISAMDVRKFQSIAKEIYTGGNIKVSFTED
                     MDNLNNSDKVVAIGSTKTISYNFQTTSEMMSNYFKIIEEENS"
BASE COUNT         4168 a         5197 c         4500 g         3880 t
ORIGIN      
        1 tgttatcttc atatttcgag ggtaacatat ctgctaatct ggtacagacc caaaaacaaa
       61 tgccgtctga aagcctttca gacggcattt ccattccaaa cgctcaaacc gccgcgtccg
      121 aacgttcgcc cgtgcggata cggattgcct cctcgaccgg cagcacaaaa atcttgccgt
      181 cgccgatttt gcccgaacgc gccacctcga caatcacgtc aatcgcgcgt tccacggcat
      241 catccgccaa caccaactcg attttgacct tgggcaggaa atcgacggcg tattccgcac
      301 cgcgatagat ttccgtatgc cccttctgcc tgccgaaccc tttgacctcg ctgacggtca
      361 tgcccgtaat gccgatttcc gtcaacgcct cgcgcacgtc gtcgagcttg aacggtttga
      421 caatcgcctc gatttttttc ataaaatttc ctttgaacaa acaatacaaa cacatccgaa
      481 aaacgggaac cccccgtcag attgtcaaca ttttaaacca aaatacccaa gcaatacagc
      541 ccccgttgcg catataatga cagcttttcc aaccgcattt gagagccgaa tccatgtctg
      601 ttgttttgcc cttgcgcggc gttaccgccc tttccgattt ccgtgtcgaa aaactcttgc
      661 aaaaagccgc cgcactcggc ctgcccgaag tcaaattaag cagcgaattt tggtatttcg
      721 ccggcagcga gaaagcactt gatgccgcga ctgtcgaaaa actgcaagcc ttgttggcgg
      781 cgcaaagcgt tgaacaaacg cccgaagcgc gcgagggttt gcatttgttt ttggtaacgc
      841 cccgtttggg tacgatttcg ccgtgggctt ccaaggcgac caatatcgcg gaaaactgcg
      901 gtttggcagg catcgaacgc atcgagcgcg gtatggcggt gtggctggaa ggtgcgctta
      961 ccgatgaaca gaaacagcaa tgggcggcgt tgctgcacga ccgcatgacc gaatccgtgc
     1021 tgcccgattt tcagacggca tccaaattat tccaccatct caaatccgaa accttctcca
     1081 ccgtcgatgt tttgggcggc ggtaaagagg ctttggtcaa agccaatacc gaaacgggtt
     1141 tggcactctc cgccgacgaa atcgattatc tggttgaaaa ctatcaggct ttgcagcgca
     1201 atccgtccga tgtggagctg atgatgttcg cgcaggcaaa cagcgaacac tgccgccaca
     1261 aaatcttcaa cgccgatttc atcctcaacg gcgaaaaaca accgaaatcc ctgttcggca
     1321 tgatacgcga cacgcacaac gcgcatcccg aaggtacggt cgtcgcctat aaagacaatt
     1381 cgtccgtgat caaaggcgcg aaaatcgagc gtttctatcc gaatgcagcg gaaaaccaag
     1441 gctaccgttt ccacgaggaa gacacccaca tcatcatgaa agtggaaacg cacaaccacc
     1501 cgaccgccat cgcgccgttt gcgggcgcgg caacaggcgc gggcggcgaa atccgcgacg
     1561 aaggtgcgac gggcaaaggt tcgcgtccga aagcgggctt gaccggcttt accgtgtcca
     1621 acctcaacat ccccgacctc aaacagccgt gggaacaaga ctacggcaag ccggaacaca
     1681 tttcctcgcc gttggacatc atgattgaag gcccgattgg cggtgcggcg ttcaacaacg
     1741 aattcggccg cccgaacctc ttgggctact tccgcacctt tgaagaaaaa tttgacggtc
     1801 aggttcgcgg ctaccacaaa ccgattatga ttgccggcgg tttgggcagc attcaggcgc
     1861 agcagacgca caaagacgaa atccccgaag gcgcactgct gatccaactg ggcggcccgg
     1921 gcatgctcat cggcttgggc ggcggtgcgg cttcttcgat ggataccggt acaaatgatg
     1981 cgtctttgga cttcaactcc gtacaacgcg gcaaccctga aatcgaacgc cgcgcgcagg
     2041 aagtcatcga ccgctgctgg cagctcggcg accaaaaccc gattatctcc atccacgacg
     2101 tgggcgcggg cggcttgtcc aacgctttcc ctgaattggt caacgatgcc ggacgcggcg
     2161 cggtattcga gctgcgcgaa gtgccgttgg aagaacacgg tttaacgcct ttgcaaatct
     2221 ggtgtaacga atcgcaagag cgttatatct tgtcgatttt ggaaaaagat ttggacacct
     2281 tccgcgccat ctgcgaacgc gaacgctgcc cgtttgccgt agtcggcacg gcgaccgacg
     2341 acggtcattt gaaagtgcgc gacgatttgt tctccaacaa ccccgtcgat ttgccgctga
     2401 acgtcttgct cggcaaaccg cccaaaacca cgcgtaccga caaaacggtt acgccgtcca
     2461 aaaaaccgtt tcacgcgggc gatatcgaca tcaccgaagc cgcctaccgc gttttgcgcc
     2521 tgcctgccgt agccgccaaa aacttcctga ttaccatcgg cgaccgcagc gtcggcggca
     2581 tgacccaccg cgaccaaatg gtcggcaaat accaaacccc cgtagccgac tgcgccgtta
     2641 ccatgatggg cttcaacacc tatcgcggcg aagcgatgtc tatgggcgaa aaaccggccg
     2701 tcgccctgtt tgacgcgccc gcctcgggca gaatgtgcgt cggcgaagcc atcaccaaca
     2761 tcgcggcagt caacatcggc gacatcggca acatcaaact ttccgccaac tggatggcgg
     2821 cgtgcggcaa cgaaggcgaa gacgaaaaac tctaccgcac cgtcgaagcc gtttctaaag
     2881 cctgccaggc attggatttg agcattcccg taggtaaaga cagcctgtcg atgaaaaccg
     2941 tgtggcagga cggcgaagag aaaaaatccg tggtttcgcc gttgagcctg attatttccg
     3001 cgttcgcgcc tgttaaagac gtgcgaaaaa ccgttacgcc cgaattgaaa aacgtcgaag
     3061 gcagcgtatt gctgtttatc gatttgggct tcggcaaagc gcgcatgggc ggctcggcgt
     3121 tcggtcaggt gtacaacaac atgagcggcg acgcgcccga tttggacgat gcaggccgtc
     3181 tgaaagcgtt ttacagcgta attcaacagc ttgtcgccga agacaaactt ttggcgtatc
     3241 acgaccgcag cgacggcggt ttgtttgcga cgctggcgga aatggcgttt gcggcacggt
     3301 gcggtattag tgccgatata gattgcctga tggataaatt cctgccgatt catctcccgg
     3361 attttcaagg cgaccctgcc gaagacttat ctgacgaact ttataatcat gccgccatta
     3421 aaatcttatt caatgaagaa ttaggcgcgg ttatccaaat ccgccaaaaa gatagggatt
     3481 atgttgatgc ggcatttgaa acggccggct taaccgatgc ggtcagccgg attggctctc
     3541 ctgattttga caatgagttt atttctttct ttggttacgg ttatttcttg gaacaaaacc
     3601 gcgccgacct gcaacgcgcg tggcaggaaa ccagccacgc catccaacgc ctgcgcgaca
     3661 accctgcctg cgccgacagc gagttcgccc tgattggcga caacgaacgc agcgcattgt
     3721 ttgccgacgt gaagttcgac gtgaacgaag acatcgccgc gccgtttatc aacagtggcg
     3781 cgaaacctaa aatcgccatc ctgcgcgaac agggcgtaaa cgggcaaatc gaaatggccg
     3841 ccgccttcac ccgcgccgga ttcgatgcct acgacgtgca tatgtccgac ctgatggctg
     3901 gccgcttccg ccttgccgac ttcaaaatgc tggcggcgtg cggcggcttc agctacggcg
     3961 acgtactcgg cgcgggtgaa ggctgggcga aatccatcct gttccaccct gccttgcgcg
     4021 accaattcgc cgccttcttt gccgatccga acacgctgac attgggcgtg tgcaacggct
     4081 gccaaatggt cagcaacctt gccgaaatta tcccgggcgc agaaacatgg ccgaagttca
     4141 aacgcaacct gagcgagcag tttgaagcgc gcctgaatat ggttcacgtc cccaaatcag
     4201 cgtcgctgat tttgaacgaa atgcaagact ccagcctgcc tgtcgtggtc agccacggcg
     4261 aaggccgcgc cgacttcgcg cttcacggcg gcaatatttc cgccgatttg ggcatcgcgc
     4321 tgcaatatgt ggacggtcaa aaccaggtta cccaaaccta tccgctcaac cccaacggct
     4381 cgccgcaggg catcgccggc attaccaacg ccgacggccg cgttaccatt atgatgcccc
     4441 accccgaacg cgtgtaccgt gccgcacaaa tgagccggaa accggaaggc tggacggaac
     4501 tgtccggctg gtaccgcctc tttgccggcg cacgtaaagc cttgggctaa accgtctgcc
     4561 caaacaaatg ccgtctgaaa aaggttttga aacccgtttc agacggcatt ttgaccattc
     4621 tcaagccctg ccggtactca atcccgcttt accgttttcc cgcaaagttt tcagcaatac
     4681 tttccacgcc gacaatttag ggttggactg ttttccttgt ccgatcaaat ccatttgcgt
     4741 catataccag ttcatttcta acattgcacc cgctttttta tcaacaaatg ctacgccagt
     4801 ttggatgcgg tctgcttgta cggtttggta agttctgttt tgcattttgt tcgccaaatc
     4861 agggactaag gcaaacgggc gggctatgcc gaccaaatcc aaatgaccgc tggataaggc
     4921 atcttccatg gcggtttgcg aacggaatcc gccggtgata atcagcgggg cttggctggc
     4981 tgcacgggct ttttcggcgt aatcgatgaa gaaggcttcg cgtttgcggg tgctgtcttt
     5041 ggcggcgagc atttgcgggc tttcgtagtt gccaccggaa acttcaataa aatcaatgcc
     5101 catttccgac agtttttgca ccacctgcac cgattcgctt tcgtcaaatc cgcctttttg
     5161 gaaatctgcc gaattgagtt ttacgcccac caagaaatct ttgcccgcgg cagcgcgaat
     5221 agcggtgtag gtttccaaaa ggaagcgcat acggttttcc aaactgccgc cccattggtc
     5281 ttggcggcgg ttgtggtgcg gcgagaggaa ttggctgatg agataaccgt gtacggcgta
     5341 aatttgtacg cctgaaaagc ctgcctgttc ggcaattttg gcagtttgta cgaattgctg
     5401 aatcagcccg ttgatttcat cggcactcaa ttcgcgcggc ggattgataa agccattcat
     5461 gcccaccagc ggcacggcgc tcggtgcaag cggtgtttta ttgaccaccg caggcgactg
     5521 tttgcccgca tgattgattt gcatgatgag cagcgtgtcg ttttgcatgc gggctttcgc
     5581 ccattttttc agcatttcaa gggcgcggtc gtctgaaatc aacacatcgt ttatcgaacc
     5641 tttgccgctt tcagccacca tcacattgcc cgtcaccaaa acgcctgcgc cgccttctgc
     5701 ccaagcgccg tacaggcgga caagtttttc agacggctgg tcgttttggg caagttgctc
     5761 ttccatggcg gatttgaaga tacggttttt ggcggttttg ccgttttgaa aagtgaatgg
     5821 cgtgaataac atgatcgttc ctttaagttg ataagtttaa aaatgtaaac gtatttgggt
     5881 aaaaataaag ggccaacgga taaccctatt ttgaaagcgg gcaattttga gccatcaaat
     5941 cggcaagctg tttcaatctc gccaccgcat ccgccatggc aatactgtaa aaccgctctt
     6001 tgccgacctg ctccaccgcc accgctccgg cttgcagcat gatttttaaa tggtgcgaca
     6061 cagcagggcg cgacagatgc agatgctcgg tcagctcatt cacattcatc ctaccgtgtt
     6121 tccacaatac atgcaggatc tgatggcggt tttcatcgct caatacggta aaaatgggta
     6181 tgcactcgcg cattaaattc atggtttgtt gcggcatatt cgatctattg agtttatata
     6241 tttaaaaatt cgaacgtatt ttagagggaa ggtcggcagt tatcaaaggc agtttggttt
     6301 gcagtttggc aatatattgt tgctgaaagg tttttataag aaaaaatgcc gcctgaaaca
     6361 gcatgatggc gtttgctagg gttccgctgt atattaacgc ctttcggata cacctttcag
     6421 acggcatccg aaccatcaag gaacactcat gaaaattacc cccgtcaaag ccctgaccga
     6481 caactacatc tggatgatac agcacggcaa ccatgccgtc tgcgtcgatc cttccgaacc
     6541 ctcgcccgtc ttggaattcc tcgtacgcaa ccgactcatg ctcgcccaaa cctgggtaac
     6601 gcacccccat cccgaccacg aaggcggtgc ggcggcactc tggcgcggct acatggaatc
     6661 gcccgtttac ggcgaatccg acatcgaagc cgccacccac accgtaaccg ccggcacacg
     6721 attcaccttc ggcaacggtc aggttaccgt ttgggcaaca cccggccaca cagaccgcca
     6781 caccagctac cttctcgaaa cttcagacgg catacacgtt ttctgcggcg acaccctttt
     6841 ttccgccggc tgcggacgcg tgtttaccgg cacggtcgaa cagctttacg acaacttcca
     6901 acggttcaac caattacccg aaggcaccct gttctatccg gcacacgaat acaccgccgc
     6961 caacctgcgt ttcgccgccc atatcgagcc ggacaacgcc gacattcaga cggcattgaa
     7021 ggcggcggaa cacacgccca ccctgcccgt taccctcgcg cacgaacgcc gcgtcaaccc
     7081 gttcttacgg acagaaatcc ccgccgtccg ccaacgtgcc gaagccctgg tcggcaaaac
     7141 gctgaacagc ggtttggaag tattcgccgc cctgcgcgaa ctgaaaaacg cctaccgctg
     7201 acctgccctc cgaaaaatgc cgtctgaaac ccgcgtttca gacggcattt gcgttaaaaa
     7261 tagtaaaccg tttcaaaagg gagtagaata gtgctgtttc caaccctgcg cctgtaccgt
     7321 caggctttta ttatggacct tcccagttcg tttttactga acaccccttc cgattccaaa
     7381 cgacaataac catcccgccg gaatgcctcc ccgcacacgg cgggcggagc atttatgagc
     7441 atcgaacaca ctcctccgac acacgacggc gaaaccggtc aaaaccatgc cgaacgccct
     7501 tccgccgatt tcgaccgtgt ccactccctc tgcgaaatcc tcgaacctgc ttttgaacaa
     7561 atcgaaaacg gcacaccgct cgaagatgcg ccgctgcgcg acaaactgac cgagctgacc
     7621 gtcctcttgg ccgagttgca ccctgccgac gtggccgacg tcttggagtc cctgccgccg
     7681 cgcgaacgca atatcgtttg gcttctggtc aaacccgaag acgacggcga agtattgctg
     7741 gaagtatccg acgcggtgcg agaaacgctg atcgagtcaa tggacaagga cgaattgttg
     7801 gcggcggtcg atgatttgga tgcggacgaa ttggcggagc tggcagacga tttgccgcac
     7861 caagtggttt atgaagcctt acagacgcgc gatgaggaag agcgcgccca agtcaaggcg
     7921 gcaatgtcgt atgaagacaa ccaagtcggt gcgattatgg acttcgagtt ggtcagcatc
     7981 cgcgccgatg tcgcctgcga agtggtgctg cgttatctgc gccgcttcga cagcctgccc
     8041 gaccataccg acaagatttt tgtcgtcgat gaaaacgacg tgctgcaggg cgtgctgccc
     8101 atccgcaaac ttttggtcgc cgatcccgaa gatttggtgg aaaacgtgat ggcgaaagat
     8161 gtcgtgcgtt tccgcgccga ggacgacgtg gaagaagcgg cgcaggcgtt tgaacgctac
     8221 gacttggtta ccgcgcccgt cgttgatgaa aacaaaaagc tcatcggcag gattaccatc
     8281 gacgagatgg tggacgtgat ccgcgaagaa tccgaagcgg atatgttgaa catggcaggt
     8341 ttgcaggaag aggaagacct gttcgccccc gtcttggatt ccgtcaaaaa ccgctggatg
     8401 tggctcgccg tcaacctctg caccgccttc ctcgccagcc gcgtcatcgg cgcgtttgaa
     8461 ggcagtatcg aaaaaatcgt cgcactcgcc gcgctgatgc ccatcgtcgc cggcatcggc
     8521 ggtaactcgg gcaaccagac gattaccatg attgtccgcg cgatggcgat ggggcagctg
     8581 acggatatgc aggcgggaag gctgttgaaa aaagaagtcg gcgtcgcctt ggtcaacggc
     8641 attatttggg ggacggtgat gggagcggtt tcgtggctgc tttacggcag cctcggcatc
     8701 ggtttggtca tggttgccgc gatgacgctc aacctcctgc tggcggcaac ggtcggcgta
     8761 ttgattcccg tggtaatgga aaaattcgga cgcgatcccg cgctgggcag ctcggtgctg
     8821 attaccgccg ttaccgactc cggcggcttc ctcattttct tgagtctcgc caccctgttc
     8881 ctgctttaaa tgccgtcccg acccgcgcaa aaatgccgtc tgaagcagaa gctgcttcag
     8941 acggcattcg accattcatc cttgttgctc aagattattg gacggtatgt cggggcagcc
     9001 ctttggcaac gccgaccaca tcctccccga acagcgcgtt gacatcggtt tcatcaaaca
     9061 cgtatttgct gtggcagaag tcgcaatcga cttcgatgct gccttgttcc gccaccacgc
     9121 cgccgacttc ttccccgccc agcatcaaca gcatatcgct gaccttgccc cgagaacagg
     9181 tacatgagga ttcaaacgtt tccggctcga acacgcgcgg cggcgtttcg tggaacaggc
     9241 ggtataaaac gtgttgcgcg tccaattctg ccagctcctc cgccgtcagc gtgcgcgcca
     9301 gcgtactgac gtgttcccat gcctcttcat ccaacacctc ttcaggcaga cgctgcacca
     9361 gcagcccgcc cgccgcttca tcgcttgcag acagggcgat gtgcgtatca agctgttcgg
     9421 aacgtttcat atagttcacc aacatttgcg cgatgctgcc gccttccaaa ggcactacgc
     9481 cctgccaggg ttcgccgtct ttgggttgca gcgtcaatac gaacacgccg ttgccgccca
     9541 aaaggtcgcc gaggctttcg tcatcggcta tttctgcggt ttcgtcccac cgcgcggttg
     9601 cacggacggt acggtcggaa gtcgcttccg caaccagcat tttcagtttt ccctgcccct
     9661 gaacctgcac aatcagcgtg ccttcgtttt taaggttgcc cgacagcaga acgccggcag
     9721 ccagtagctc tcccaaggcg cagcggatgg cggcgggata gtttttctgt tttacaatgt
     9781 gctgccacac gttttccaga cggacgtgca gcccgcgcac gggcatatcg tcgaagataa
     9841 agcgggtacg cgcatcggcg cagttgatgg cggtttgatt catgattttc tctgactgat
     9901 tgttcggatg gcggctatat ggttgcggtc ggcgcgaaaa caaggcggac ggcggatgcg
     9961 cttcccaaat tctcaataaa ttatataaaa atcaacatat taactcaatc taacaagccg
    10021 ttttttgcca aacagccgtt tttttatata caatcaacaa gatattttcg actgatacag
    10081 cataacatcg cacggcggca cgatgcctcc tgcgcggaaa caccgatatg gattcttttt
    10141 tcaaaccggc agtttgggcg gttttgtggc tgatgtttgc cgtccgcccc gcccttgccg
    10201 acgagttgac caacctgctc agcagccgcg agcagattct cagacagttt gccgaagacg
    10261 aacagcccgt tttacccgtc aaccgagccc ccgcccggcg ggcgggcaat gccgacgaac
    10321 tcatcggcag cgcgatgggg cttaacgaac agcccgtttt acccgtcaac cgagcccccg
    10381 cccggcgggc gggcaatgcc gacgaactca tcggcagcgc gatggggctt ttgggtattg
    10441 cctaccgcta cggcggcaca tcggtttcta ccggttttga ctgcagcgga ttcatgcagc
    10501 acatcttcaa acgcgccatg ggcatcaacc tgccgcgcac gtcggcggaa caggcgcgga
    10561 tgggcgcacc cgttgcccga agcgaattgc agcccgggga tatggtgttt ttccgcacgc
    10621 tcggcggcag ccgcatttcc catgtcggac tttatatcgg caacaaccgc ttcatccacg
    10681 cgccgcgcac ggggaaaaat atcgaaatca ccagcctgag ccacaaatat tggagcggca
    10741 aatatgcgtt cgcccgccgg atcaagaaaa acgacccgtc acgctttctg aactgatttc
    10801 ccaaggaata cgcaatgagt atgcccgaaa tgcccaaatg gtacggcgat gacggacaga
    10861 tcgtatcctg taccgaaaag gtcaaagtga tgtccgaaaa tatggccgag ctgtatcaga
    10921 cggcacaaga cgcgtttgaa gacgcgctgc tgatgggttg cggcgaacgg cagttgcgcg
    10981 cttacctgct cgcgctgatt gaaggtttgg aaaatcccta ccgcaaagtc tgaacacgcc
    11041 ccggttgctg cggcacggtt tatccgtgcc gtttttgcgt ttgtgcgcgg cttcggcttt
    11101 tcagacggca tatttgacgt tatgattaaa cagttaacaa gatttatcac aacgccgtca
    11161 aaagacagac acacaacatg aacatcatcc gcgcgctcct catcatcctc ggctgcctcg
    11221 ccgccggcga aaccgccgtt ttcctagcag gcatcaaact gcccggcagc atcgtcggca
    11281 tgggcgtgct gtttgcgctt ttgcaggcgg gttgggtcaa aacgtcttgg ctgcaacagc
    11341 ttaccgacgc gctgatggca aacctgacgc tgttcctcgt gccgccctgc gtggcggtca
    11401 tcagctattt ggatttgatt gccgacgatt ggttttcgat actggtttcc gcctccgcca
    11461 gcactttgtg cgtactgctg gttacgggca aggttcaccg ctggatacgg agcattatct
    11521 gatgagcgaa atcctcaggc agcccggcat cctgcttttc ctcacgcttg ccgtgtacgc
    11581 gcttgcgatt atcgtgcgca cgcgcacggg caatatcttc tgcaaccccg tactcgtcag
    11641 cactatcgtg ctgattgcct acctgaaaat cctcggtatc gattatgcgg tgtaccacaa
    11701 cgccgcgcaa ttcattgatt ttcggctgaa acccgccgtc gtcgtgcttg ccgtgccgct
    11761 ctaccaaaac cgccgtaaaa tcttcaacca gtggctgccc gtcatcgttt cgcagcttgc
    11821 gggcagcgtt acgggcattg ttacggggat gtattttgcc aagtggctgg gcgcggaacg
    11881 cgaagtcgtc ctcccgctcg cgtccaaatc tgttaccaac cccatcgcca tcgaaatcac
    11941 cagctccatc ggcggcattc ccgccattac cgccgccacc gtcatcattg ccggtctggt
    12001 cggacagatt gccggttaca aaatgttgaa gaacacggtc gtcatgccct cgtccgtggg
    12061 tatgtcgctc ggcacggctt cgcacgcgat gggcattgcc gcctcgctcg aacgcagccg
    12121 ccgcatggcg gcatacgcgg ggctggggct gacgttcaac ggcgtgctga ccgcgctgat
    12181 tgcgccgctg cttatccccg ttttggggtt ctgaacccgt ttcagacggc atttcaaccc
    12241 atgccgtctg aacgccgaca cactcgcaag gagaaccgtt atggctgtca acctgaccga
    12301 aaaaaccgcc gaacaactgc ccgacatcga cggcattgcc ctctacaccg cccaagcagg
    12361 cgtgaagaag cccgggcata ccgacctgac actgattgcc gtagccgccg gcagcaccgt
    12421 cggtgcagtc ttcacgacca accgtttctg tgccgcgccc gtccacatcg ccaaatcgca
    12481 ccttttcgac gaagacggcg tgcgcgccct cgtcatcaac acgggcaacg ccaacgcggg
    12541 tacgggcgca cagggcagaa tcgatgcttt ggcagtgtgt gccgccgccg cccggcaaat
    12601 cggctgcaaa ccgaaccagg tgctgccctt ctccaccggc gtgattctcg aaccgctgcc
    12661 cgcagacaaa atcatcgccg ccctgcccaa aatgcagcct gccttctgga acgaagcggc
    12721 acgcgccatc atgaccaccg acaccgttcc caaagccgcc tcgcgcgaag gcaaggtcgg
    12781 cgaccaacac accgtccgcg ccacaggcat tgccaaaggc tcgggcatga ttcatcccaa
    12841 tatggcgacc atgctcggtt tcatcgccac cgatgccaaa gtttcccaac ccgtcctcca
    12901 actgatgacg caggaaatcg ccgacgaaac cttcaacacc atcaccgttg acggcgacac
    12961 cagcaccaac gacagcttcg tcatcatcgc caccggcaaa aacagccaaa gcgaaatcga
    13021 caacatcgcc gacccgcgtt acgcccaact caaagaattg ttgtgcagcc ttgcgctcga
    13081 actcgcccaa gccatcgtcc gcgacggcga gggcgcgacc aagttcatca ccgtccgcgt
    13141 cgaaaacgcc aaaacctgcg acgaagcccg ccaagccgcc tacgccgcgg cacgttcgcc
    13201 gctggtcaaa accgcctttt tcgcctccga ccccaacctc ggcaggctgc tcgccgccat
    13261 cggttatgcc gacgttgccg acctcgatac cgacctcgtg gaaatgtatc tcgacgatat
    13321 tttggttgcc gaacacggcg gacgcgccgc aagctacacc gaagcacaag ggcaggcggt
    13381 gatgtcgaag gacgaaatca ccgtccgcat caagctgcat cgcggacaag ccgccgccac
    13441 cgtctatacc tgcgacctgt cgcacggata cgtttccatc aacgccgact accgttcctg
    13501 acccgacacg gcttcagacg gcatacataa aatgccgtct gaaccgtcgg acaacatacc
    13561 atgacctcca cattcccccg ccgcctcgcc cgcaaaatcc gccaaacccg ccgcctgtcg
    13621 cgcaaaagca tcgcctttct gttccttctg gcaggttcgg cactcgtcgc cctgaccgca
    13681 ctgttttttg cccatcttgc cgattttgcg ctggaactga acgccaaact ggttcaacaa
    13741 tacccgtggt tcgcatggat tgccctgccg ccgggactgc ccgtcatcgt ctggctcaca
    13801 cgcaaattcg cccccttcac cgccggcagc ggcatccctc aagtcatcgc ctcactgtcg
    13861 ctgccctacg gcgcacaaaa aacgcggctg atacgcctcg ggcaaacgct gctgaagatt
    13921 ccgctgacct ttttgggtat gctgttcggc gcgtccatcg gacgcgaagg cccgtccgtg
    13981 caggtcggcg cggcagtaat gggcgcgtgg ggcgcgtggt gcaaaaaaca cggtttggcg
    14041 tttaagggta tgcaggaaaa cgatttgatg gcggcgggcg cggcgggcgg tttggcagca
    14101 gcgttcaacg cgccgctggc gggcgtgatt ttcgccatta aggaactggg gcgcggcatc
    14161 atgctgcgct gggagcggca gattctgctc ggcgtactcg ccgccggttt catacaggtc
    14221 gccattcagg gcaacaaccc gtatttttcg ggtttcaacg gcggcgtttt ggaaaatatt
    14281 ttgatgtgga tcgtggcggc ggggctggtt tgcggcgcgg ctggcggcat atttgcccgt
    14341 atgctccatc gcggtgcggc ggcgtttgca ccgcgcaaga tacgcggctt catccgcaac
    14401 cgtccgctgc tgctggcggc actgatgggg ctgctgctcg ccctgctcgg cacgttctac
    14461 caaggcaaaa cctacggcac cggctaccac gaagccgccc aagccctgca cggcatctac
    14521 gaagcccccg tcggactcgc cgccgccaaa tggctcgcca ccgtattcag ctattgggca
    14581 ggcatcccgg gcggcatttt cactccctcg ctgaccatag gtgcggtttt gggcgagcat
    14641 atcgccgcca tcgccgacat atcgcagggt gcaaacatca tcgtcctcat ctgcatggcg
    14701 gcatttttag cgggcgcgac acaatcgccg attacttccg ccgtcgtcgt catggaaatg
    14761 accggcgggc aaagcctgct gttttggatg ctgattgcct gcattttcgc ctcgcaggtt
    14821 tcgcgccagt tttcgccgcg tccgttctac cacgcatcgg gaatgcgctt ccgccggcgc
    14881 gtgcttcagg aaaccgccgc ccaaaccggc aatgcgcccg caagaccgca agccgcaaac
    14941 agcaaaacgg gaatgccgtc tgaaaattaa aacgcccccg atcaaacgcc ggcagccgcc
    15001 ttgattcaaa taccgttccg ctgcgcttga aatttcagca acaatgccgt ctgaacgaca
    15061 gaatgcggtt ttcagacggc atttccccat cccgatattg ttcaaacaaa accgaagcgt
    15121 ttgctataat tgcacttttt accgcacacg cacgcccatg tttcccgatt tctcccaaac
    15181 cctctccaaa gaccgccact tcctgcaatc cgccttcaaa aatcccaaca aatacggcgg
    15241 cttgtccaaa atcgaagaaa aataccgaaa atcgcacgaa atcttcttga aacgtttggc
    15301 cgcattgcca aaacccgaat tcgacaacac cctgcccgtt cacgaaaaac tcgaagaaat
    15361 caaaaaaacc gttgccgaaa atcaggtaac gattatctgc ggcgaaaccg gttcgggcaa
    15421 aaccacgcag ttgcccaaga tttgtttgga actcgggcgt ggggcggcag gattgatcgg
    15481 gcatacccag ccgcgccgtt tggccgcgcg ttcggtggcg gagcggattg ccgaagagct
    15541 gaaatccgaa atcggcagcg cggtcggcta taaagtgcgc tttaccgacc acacctcgcg
    15601 cgatgcctgc gtcaagctga tgaccgacgg catcctgctg gcggaaaccc agaccgaccg
    15661 ttatctcgcc gcctacgaca cgattatcat tgacgaagcg catgaacgca gcctgaacat
    15721 cgacttcctc ttgggctacc taaaacaact cctgccgcgc cgccccgatt tgaaagtcat
    15781 catcacatca gccaccatag acgcagaacg cttctcccga cacttcaacg gcgcgcccgt
    15841 actggaagtg agcgggcgca cctatcccgt cgaaatcctc taccgaccgc tgaccggcaa
    15901 agacgaagac gacgcagaag tcgagctgac cgacgcgatt gtcgatgccg ccgacgaatt
    15961 ggcacgctac ggcgaaggcg atattttagt attcctgccg ggcgaacgcg aaatccgcga
    16021 agcggcagaa gccctgcgca aatccacact gcgccgcaat gacgaaatcc tgcccctgtt
    16081 tgcacgcctg tcgcacgccg aacagcacaa aatcttccac ccttcaggcg caaaatgccg
    16141 catcgtgctg gcgaccaacg tcgccgaaac ctcgctcacc gtgccgggca tcaaatacgt
    16201 catcgacacc ggcctcgcgc gcgtcaaacg ctattccgcg cgggcaaaag tggaacaact
    16261 tcacgtagaa aaaatctccc aagccgccgc ccgccaacgc tccggccgct gcggacgcgt
    16321 ctcagcaggc gtgtgtatcc gactgttttc agaagaagat tttaacagcc gtcccgaatt
    16381 taccgatccc gaaatcgtcc gcagcaacct cgccgccgtc atcctgcgca tggcatcgct
    16441 gaacttgggc gacgtagcag cattcccgtt tttagaaatg cccgactcgc ggtatatcaa
    16501 tgacgggttt caggtgttgt tggaattggg ggcagtggag gttgtctgaa aacaggcaga
    16561 tcgggcgttt atgcccaacg agaaccttat tgtcactatt attagggatt aatacaatga
    16621 atataaaaga atttatgtct aactatacca accatcccgt tctctttatt ggaacaggta
    16681 tgagtttgag atacttagat aattcatata cttgggatga tttattatct aaaattgcaa
    16741 tagatttatt tggagatgat agggaatatt tgaacatcaa atcacggtac tgtgaagatg
    16801 gtagattcca atatgaagag attgcagagg aattacaaag taaatttgat aaagttttag
    16861 aaaatgaccc tgatggtaga tttaaagaaa taaatgataa gttctttgaa aatatgaggg
    16921 cgggaaacac cttgagcagg tttaagattt atatatccac cctgctctca caattgaatt
    16981 ataaagataa ttctaataca gaattatctg aattaaaaaa agcgagaaaa aatgtagggt
    17041 caatcattac aacaaattat gataaattag cccaagatat ttttgaattt aacccactaa
    17101 ttggtaatga tattctttta agcaaccctt atggctcagt atacaaaata catggttgtg
    17161 tggacgatcc atcaaaaatt attattacca aaaaggatta tgaaaaattt aaagaaaaat
    17221 atgaacttat tagagcccaa ctattatcgt tatttattca caatccaatc atatttcttg
    17281 gatataatgt tggtgacgaa aatatcaaag aaattttaaa aacaatcttt acttatgtag
    17341 aacaaaactc tccttcagct aataaaattc gtaggaattt tttactcgta gaatatgaac
    17401 ctgagtctaa caatgaagat attgttgaac atgatataga tataactgga ttctctacta
    17461 tccgtattaa taaaatcaaa acagataact tctcacaaat ttataaagct cttgcagaac
    17521 taacactacc aatctcagct atggatgtac gtaaatttca atctatagca aaggagattt
    17581 atactggcgg taacattaaa gttagcttta cggaagatat ggacaattta aataatagcg
    17641 ataaagtggt tgctattggt tcaactaaaa ctatcagcta caactttcaa acaacatcag
    17701 agatgatgtc aaattatttc aaaataattg aagaggaaaa ttcac
//
LOCUS       DAXFWM010000048        17695 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000048 DAXFWM010000000
VERSION     DAXFWM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17695
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.048"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(633..1475)
                     /locus_tag="ACFI8B_001480"
     CDS             complement(633..1475)
                     /locus_tag="ACFI8B_001480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3834673.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1710..2630
                     /gene="pyrB"
                     /locus_tag="ACFI8B_001481"
     CDS             1710..2630
                     /gene="pyrB"
                     /locus_tag="ACFI8B_001481"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC3834674.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSCVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2640..3098
                     /gene="pyrI"
                     /locus_tag="ACFI8B_001482"
     CDS             2640..3098
                     /gene="pyrI"
                     /locus_tag="ACFI8B_001482"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC3834675.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            3161..3827
                     /locus_tag="ACFI8B_001483"
                     /pseudo
     CDS             3161..3827
                     /locus_tag="ACFI8B_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4232..5455)
                     /locus_tag="ACFI8B_001484"
     CDS             complement(4232..5455)
                     /locus_tag="ACFI8B_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC3834676.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     GISGKYLYSPWQWCRLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KISPDKEVSGYGIPAIDVNFYRVFMQHPQIVSRKETAAAPRLLSGPEGRLLYTKGARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
     gene            5639..5794
                     /locus_tag="ACFI8B_001485"
     CDS             5639..5794
                     /locus_tag="ACFI8B_001485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834677.1"
                     /translation="MALLNIEQGYNTTLHNRSPYLINFGLSCLIGLEKAAAAPRDVPR
                     DNKCSAF"
     gene            5867..6370
                     /gene="def"
                     /locus_tag="ACFI8B_001486"
     CDS             5867..6370
                     /gene="def"
                     /locus_tag="ACFI8B_001486"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC3834678.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            6458..7384
                     /gene="fmt"
                     /locus_tag="ACFI8B_001487"
     CDS             6458..7384
                     /gene="fmt"
                     /locus_tag="ACFI8B_001487"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC3834679.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            7463..8722
                     /gene="rsmB"
                     /locus_tag="ACFI8B_001488"
     CDS             7463..8722
                     /gene="rsmB"
                     /locus_tag="ACFI8B_001488"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC3834680.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAVLADMPCTASGVARRNPD
                     VKWLRRPTDAAKTARRQEALLDALWQVLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            8688..9287
                     /locus_tag="ACFI8B_001489"
     CDS             8688..9287
                     /locus_tag="ACFI8B_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC3834681.1"
                     /translation="MAFITRLFKSIKQWLVLLPILSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            9287..11407
                     /locus_tag="ACFI8B_001490"
     CDS             9287..11407
                     /locus_tag="ACFI8B_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3834682.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLACYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDVQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            11400..12677
                     /locus_tag="ACFI8B_001491"
     CDS             11400..12677
                     /locus_tag="ACFI8B_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC3834683.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            12768..13955
                     /gene="dprA"
                     /locus_tag="ACFI8B_001492"
     CDS             12768..13955
                     /gene="dprA"
                     /locus_tag="ACFI8B_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC3834684.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            13981..14442
                     /locus_tag="ACFI8B_001493"
     CDS             13981..14442
                     /locus_tag="ACFI8B_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC3834685.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            14520..16826
                     /gene="topA"
                     /locus_tag="ACFI8B_001494"
     CDS             14520..16826
                     /gene="topA"
                     /locus_tag="ACFI8B_001494"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC3834686.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPGNGFAMKYQLISRNAKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPDEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQVELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            17047..17175
                     /locus_tag="ACFI8B_001495"
                     /pseudo
     CDS             17047..17175
                     /locus_tag="ACFI8B_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            17165..17695
                     /locus_tag="ACFI8B_001496"
                     /pseudo
     CDS             17165..17695
                     /locus_tag="ACFI8B_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         4237 a         4741 c         5089 g         3628 t
ORIGIN      
        1 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
       61 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccata atcatgcaac
      121 ctatattaat aaatagctaa ctaattctat tacagaaatt agttagctga ttaaaagtga
      181 ttaacaatta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
      241 aatagtacga aaccgattcg cttggtgctt cagcacctta gggaatcgtt ctctttgagc
      301 taaggcgggg caacgccgta ctggtttttg ttaatccact ataaatagcg agcatcgatt
      361 cacggtgcgc tttagtgcaa agcgtacaac gcgagcctga accaccagcc gcaataggaa
      421 aagaaagccg atagagtgca atgcttgcca acgcgcaagc gagcttgcaa gaacgcttgg
      481 ctcaacgaga gcaggcaaga cagaaagcag gataggagag gcaacgcaaa ggtctcgggc
      541 tttgatttcg ccgtaaacct gagtgccgcc ttgtccggac ggggtggttc aggcggcggg
      601 tgccgacagg gtgcagatga ggaggagtat tttcatttgg ggtcgcaacg gaagtggtag
      661 gcgcagattt caaaaccgtt tttgaaatac aggcggtgcg cgtcggtacg gtcgtggttg
      721 acgtggacgt taaggtggat tttggtaacc cctgtttccg cgctgatttt gcggacttct
      781 tccaaaaggc gcgaggcgta gcctttgcgg cggctttgcg gcagggtaac gatgtcgtcg
      841 atgtggatgt ggcggccgcc ggcgagggtg caggcttcgc ggaagccgca gacagcgacg
      901 gcgttgtgtt tgccttcttc aaaaatgccc agcaggcggt agccttgggg gcgttgggtt
      961 ttgttgatct gttcggtaaa gcggccgatg tcggtcaggg cggaacgcaa aacgctcaag
     1021 gctgcaaagg cggtggcggt gtcgtccgcg ccgatttcgc gtaaaacgta ggatgcgccc
     1081 gaggcggtct gttcctgtgt tttctcggcg gcgtgttttt cttcgattgc ctgtgccagc
     1141 aggacgcgtt cgttggcagg gtcgttttgt ccgccctgtt cgcgttcttc gagcagggct
     1201 ttgcagtcga tgacgcgcag gtcgttgtcg gcggcaaagt ccatcaggaa gcggaacatc
     1261 tgcggattgt cggtttccag ttttttatcg acggcgacgc agcggatgtt gtccaccagc
     1321 atggggcgca cccatttcga ataggagagg cggttgtctt tggtgaacga ggacaggatg
     1381 ccgcacaggc gttccgccca gtcgccgggg cggaaaatct tgccggaact cgttgtgccg
     1441 tggatgacga cttcgtaggg gttgcagatt aacatggcgg cttcctgaaa agaaatgtct
     1501 ggcgcgatta taccttatgc ttatgcgggc gtgtttggat atgccgtctg aaaaatacag
     1561 gattcgtgcg gtaaaacttt gtggaggcaa atgtgcgata atacgcgccg tgtcgccgct
     1621 tttgcgaagc tgttccgcaa acatacgggc ggcgtggacg tataaccgga tgcccgcctg
     1681 acgcgggttt tttacggaag gggggcaaaa tgcctaatcc gctttacaga cagcacatca
     1741 tctccatttc ggatttgtcg cgcgaacagt tggaatgcct gcttcagacg gcattgaagc
     1801 tgaaggcgca tccgcgcggc gatttgttgg aaggcaaact catcggttcg tgctttttcg
     1861 agccgtccac gcgcacgagg ctgtcgtttg aaacggcggt gcagcgtttg ggcggtaagg
     1921 tcatcggctt ttcggacggc gcgaatacca gtgccaaaaa aggcgaaacg cttgccgata
     1981 ccgcccgtat catttccggc tacaccgacg cgattgtcca acgccatccc aaagacggcg
     2041 cggcgcgcgt ggcggcggag ttttcgtgcg tccccgtcat caacgccggc gacggcacga
     2101 accagcaccc tagccaaacc ttgttggacc tggtaacgat ttatgaaacg caggggcgtt
     2161 tggacaagct taaaatcgcc atggcgggcg acttgaaata cgggcgcacc gtgcattcgc
     2221 tgtgtcaggc gttgaaacgc tggggctgcg aatttgcctt cgtctcgccg cccagcctcg
     2281 cgatgcccga ctacattacc gaagagttgg aagaagccga ctgccgatac cgcgccctcg
     2341 gcagtttgga agaggcggcg gaatgggcgg acatcttgta tatgacccgc gtccagcgcg
     2401 aacgtttcga cgaacaggaa tttgccaaaa tccaaggcaa attcaacctc gaagcgtcta
     2461 tgctcgcccg cgccaaaccg aacctgcgcg tgctgcaccc cctgccgcgc acggacgaaa
     2521 tccaccccga tgtcgatgcc gcgccgcacg cctattattt cgagcaggcg accaacggcg
     2581 tttatgcgcg tatggcgata ttgtcgctgg tgttgaacga agaagtgtga ggaaccgata
     2641 tggaagcgca aaaactcagt gtcgaagcca tcgaaaaagg cacggttatc gaccatattc
     2701 ccgccggcag ggggctgacc atcctgcgcc agttcaaact tttgcactac ggcaacgcgg
     2761 taaccgtggg cttcaacctg cccagcaaaa cccagggcag caaagacatc atcaaaatca
     2821 aaggcgtgtg cttggacgat aaagccgccg accgcctcgc cctgttcgcc cccgaagcgg
     2881 tggtcaacac catcgacaat ttcaaggtcg tacagaagcg gcatttgacc ctgcccgacg
     2941 aaatcgccga agtgttccgc tgcccgaatc ccaattgcgc cggccacggc gagccggtca
     3001 aaagccggtt ttatgttaag aaacacaacg ggcagacgcg gctgaaatgc cactactgcg
     3061 aaaaaaccta caaccgggat tcggtggcgg aagcctgacg gattcccgca gcccggcggc
     3121 ggaattttgc ctgccgcctg ttttgccaat ctgaaatgga atgatgatgc acgcttctgt
     3181 ccaaagtcgt ttcgcaccga tactttatgt tttgattttc tttgccggtt ttttgaccgc
     3241 gcaaatctgg ttcaatcaga aagcctatac tgaagagctg cctccgcttc tgtccgcatt
     3301 gtccgccgtc gcgctggtgt ggctggcgtg ggcgttcgtg tcggtgcgtt caaaggctaa
     3361 ggcagaaaag ttctaccgcg aaaaaatgat acagaacgaa agcatacacc ccgtcctgca
     3421 cgcttctttg caacacttgg aacacaagcc gcaaatgctc gccctgctgg tcaaaaacca
     3481 cggcaaaggc atggcggaac aggtcaggtt caaggcggaa gtgctgcccg acgacgaaga
     3541 cgcgtgcacg attgccgccg agttggcaaa aatggatatg ttcgcattgg ggacggacgc
     3601 ggtcgcctcg ggcgaaacct atgggcgcgt gttcgccgat attttcgagt tgtcggcggc
     3661 tttggaaggg cgcgcgttca aagggatact gaaactgacg gcggaatata aaaaacatct
     3721 tcggcgatgc ctgccgttcg gaaacggcgt tggatttggg cgcgctcaat caggcgttga
     3781 gggaaatctc gaaaacgccg gaaaagccta agcagatatt ttattgaagg tgtcccgatg
     3841 tatggggagc gcacgggtgg cgcggcggca aagcggcaag attgggtttg gatacccggt
     3901 tggttttata gtagattaac aaaaatcagg acaaggcggc gggccgcaga cagtacaaat
     3961 agtacggaat cggttcgccc ggtgcttggg caccttaggg aatcgttccc tttgagccgg
     4021 ggcggggcaa cgccgtaccg gtttttgtta attcgctata tctgtctgca agggagacag
     4081 ccgctgtgat ttccgttcgg gcggaaaccg attccatcgg ataaaaggca ttttgtccga
     4141 ctaaatcagg gttttgcatc aacgggatat gaaaacacag cctaaataaa aatgccgtct
     4201 gaatgcttca gacggcattg ttgccgaggg gttattggaa agggtcggaa tcgacgcggg
     4261 cgcggccctg atccggcata ttgtccaaat cccgtcccgg attggcggcg gtgtcgcctt
     4321 cggaaatatc ggagatgttt tccaaaataa tggcgtaagc cagtttcggc gcagtgtggt
     4381 aaaccattgc caagcccact tcttcggcag gtgtcgaaat cagctcgacg gtatccctgc
     4441 ttttaggttc ttcggtcaaa ttgttggaga ggttgacctg catcgttttc ttgcgtttgt
     4501 aaaggcttaa aactgcgcct ttgtccaaac cgtcgtcccc gcctttgtct atcgtaatgg
     4561 ttttgaactg tccgccgacc cccacgcctt caaacacgga aacgattttt gcctgaaccg
     4621 ggcgggacgg ttcgtgcggc accatattga agcggtcggt gtcttccggc attttcatca
     4681 ggtagtcgcc ctgctgtatt tcggaaatgg cggtttcgac caccagcggc tgtatcgaac
     4741 gggtgcgcac cggggtaatc aaaggatggg tgcgggtgta gtattcgttg tctttcagcc
     4801 gttcttcagc ctgtttcgag cgttgttcga gggcggagtc ggtatagtcg agggagcgca
     4861 cgatgccgct gaatgcgact tcttgtccga ggaatttacc cgtatccgga tcggtgatgt
     4921 ttttattgat tcggtaggtc agataacggc ccggctcttt caggcctttg gtgtaaaccc
     4981 tggcgccttt ggtgtacagc agcctgcctt ccgggcccga gagcaggcgc ggcgcggcag
     5041 cggtttcttt gcgggaaacg atttgcgggt gctgcataaa gacgcggtag aagttgacat
     5101 cgatggcggg aataccgtat ccggacactt ccttatccgg actgattttg acgacgggga
     5161 tgccgtctgt ctgttccaaa tcaaggcgcg gttcgccgcc aacgtagcgc aacaccaata
     5221 cctggcccgg ataaatcagg tcgggattgt ggatttgatc ccggttcgcg ccccacaggc
     5281 ggcaccattg ccacgggctg tacaggtatt tgcccgaaat accccacagg gtgtcgccct
     5341 gtttgaccgt gtagcgttgc ggcgcatccg ggcgcacctc cagatttgcc gccaaagttt
     5401 gtgttgagaa tgccatacct gccgcgcaga gcagggttat aatacgacgt tgcataaccg
     5461 ttccccttat ctgataaatt tcggtttgtc ttgcttgatt gggttggaaa aagcggcggc
     5521 agcccctcgg gatgtgccgc atgataacaa atgttccgca ttttaacatc gaattatccg
     5581 taccatcacg gtaattatga aaaacaggcg gcgtatccgc cgaaggaaag agaaaattat
     5641 ggctttactg aatatagagc agggttataa tacgacgttg cataaccgtt ccccttatct
     5701 gataaatttc ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag cccctcggga
     5761 tgtgccgcgt gataacaaat gttccgcatt ttaacatcga attatccgta ccatcacggt
     5821 aattatgaaa aacaggcggc gtatccgccg aaggaaagag aaaattatgg ctttactgaa
     5881 tatcttgcaa tatcccgacg agcgtctgca cacggtggca aagcctgtcg aacaagttga
     5941 cgagcgcatc cggaagctgg ttgccgatat gtttgaaacg atgtacgaat cgcgcggcat
     6001 cgggctggcg gcgacgcagg tcgatgtgca cgaacgcgtg gtcgtgatgg atttgaccga
     6061 agaccgcagc gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag acggcgaaac
     6121 cacttacgaa gagggctgcc tgtccgtacc gggcatttac gacgccgtta cccgcgccga
     6181 acgcgtcaag gtcgaggctt tgaacgaaaa aggcgaaaaa ttcacgctgg aggcggacgg
     6241 gctgctggcg atttgcgtgc agcacgagtt agatcacctg atgggcatcg tgtttgtcga
     6301 acgcctttcc caactcaagc aggggcggat taagaccaaa ctgaaaaaac gtcagaaaca
     6361 tacgatttga ttcgcttcgt catgccgtct gaacgctgca aggctttcag acggcacagc
     6421 cttgtccgac aactttacgc acacgcagga acacgctatg aaagtcatct ttgccggcac
     6481 gcccgatttt gccgccgccg ccttaaaagc cgttgccgcc gccggttttg aaatcccgct
     6541 ggtgctgacc cagcccgacc gtccgaaagg gcgcggtatg caactgaccg ccccgcctgt
     6601 caaacaagcc gcgctggaac tcggtttgcg cgtggcacag cccgaaaaac tgcgcaacaa
     6661 cgccgaagcc ctgcaaatgc tcaaagaggt cgaggcggac gtaatggtgg ttgccgcgta
     6721 cggcttgatt ctgccgcagg acgtgttgga tacgccgaaa cacggctgcc tcaatatcca
     6781 tgcttcgctg ctgccccgtt ggcgcggcgc ggcgccgatt cagcgcgcga ttgaggccgg
     6841 cgatgccgaa accggcgtgt gcattatgca gatggacatc ggtttggaca ccggcgatgt
     6901 ggtcagcgaa caccgctacg ccatccaacc taccgatact gccaacgaag tccacgacgc
     6961 gctgatggaa atcggtgcgg cggcggttgt tgccgatttg caacagcttc aaagcaaagg
     7021 ccgtctgaac gcggtcaaac agcccgaaga aggcgtaact tacgcccaaa aactaagcaa
     7081 agaagaagcg cgtatcgatt ggagcgaaag cgcggacatt atcgaacgca aaatccgcgc
     7141 cttcaacccc gtgcctgccg cgtgggtcga atatcagggc aagccgatga aaatccggcg
     7201 tgccgaagtg gtggcgcaac aaggtacggc aggcgaagtg ttgtcctgtt cggcggacgg
     7261 tttggtcgtt gcctgcggcg aaagcgcgct gaagattacc gaattgcagc ctgccggcgg
     7321 caggcgtatg aatattgcgg cgtttgcggc gggacggtct attgaagcag gggcgaagct
     7381 gtaaatccct tcagacggca ttccgatccg caaacgggaa tgccgtctga aaccatcagt
     7441 tgaagaaagc gaatcacata atatgagtat ggcacttgcc caaaaacttg ccgccgacag
     7501 cattgcggcg gttgccgaag ggcgcaacct tcaggacgtg ttggcgcaca tccgcgcggc
     7561 gcatccgcag ctgacggcgc aggaaaacgg cgcgttgcag gatatcgcct acggctgcca
     7621 gcgttatttg ggcagtttga aacatatgct cgcgcagatg ctgaaaaagc ccatcgacaa
     7681 tccgcagctc gaaagcctgc ttttggcggc gttgtaccaa ctgcattaca cgcgcaacgc
     7741 gccccacgcc gtggtcaacg aagctgttga aagcatagcc aaaatcgggc gcgggcagta
     7801 ccgttcgttt gccaacgcgg ttttgcgccg ctttttgcgc gaacgtgaaa aattggcggc
     7861 ttcctgcaaa aaagacgatg tggcgaaata caacctgccg ctgtggtggg ttgcctacct
     7921 gaaaaaccat tatccgaaac actggcacaa catcgccgcc gcgctgcaat cccatccgcc
     7981 gatgacgtta cgcgtcaacc gccggcacgg caacgccgaa agctatttgg aaaaactggc
     8041 tgtggaaggt atcgcggcta aggcgttgga cgaatatgcg gttacgttgg aagaagccgt
     8101 gccggtaaac cgtctgcccg gttttgccga aggactggtg tcggtacagg acttcggcgc
     8161 gcagcaggcg gcgtatctcc ttaacccgaa agacggcgaa cggattttgg acgcgtgcgc
     8221 cgcgccgggc ggcaagacgg ggcatatttt ggaactggcg gactgccgcg ttaccgcctt
     8281 ggacatcgat gcaggccgtc tgaagcgtgt ggaggacaat atcgcccgtt tgggctttca
     8341 gacggcatcg gcggcgtgtg ccgatgcgcg ggatttggcg gcgtggtatg atgggaaacc
     8401 gtttgatgcc gtccttgccg acatgccgtg caccgcttcc ggcgtggcgc ggcgcaatcc
     8461 cgacgtgaaa tggctgcgcc gcccgaccga tgccgccaaa accgcccgcc ggcaggaagc
     8521 cctgctggac gcattgtggc aggtgctgaa acagggcggc agaatgctgc ttgccacctg
     8581 ttccgtgttc gtcgaagaaa acgacgggca actgcaaaaa ttcctcaacc gccatgccga
     8641 tgccgaaccg atcgaatcgc gggtgctttt accgaacaaa caccaagatg gcttttatta
     8701 cgcgcttatt caaaagcatt aaacaatggc ttgtgctgct gccgatactc tccgttttgc
     8761 cggacgcggc ggcggagggc attgccgcga cccgcgccga agcgaggata accgacggcg
     8821 ggcggctttc catcagcagc cgcttccaaa ccgagctgcc cgaccagctc caacaggcgt
     8881 tgcgccgggg cgtaccgctc aactttacct taagctggca gctttccgcc ccgacaatcg
     8941 cttcttatcg gtttaaattg gggcaactga ttggcgatga cgacaatatt gactacaaac
     9001 tgagtttcca tccgctgacc aaccgctacc gcgttaccgt cggcgcattt tccaccgatt
     9061 acgacacttt ggatgcggca ttgcgcgcga ccggcgcggt tgccaactgg aaagtcctga
     9121 acaaaggcgc gttgtccggt gcggaagcag gggaaaccaa ggcggaaatc cgcctgacgc
     9181 tgtccacttc aaaactgccc aagcctttcc aaatcaatgc attgacttct caaaactggc
     9241 atttggattc gggttggaaa cctctaaaca tcatcgggaa caaataatgc gccgcttcct
     9301 accgatcgca gccatatgcg ccgtcgtcct gctgtacgga ttgacggcgg cgaccggcag
     9361 caccagttcg ctggcggatt atttctggtg gatagtctcg ttcagcgcaa tgctgctgct
     9421 ggtgttgtcc gccgttttgg catgttatgt catattgctg ttgaaagaca ggcgcaacgg
     9481 cgtgttcggt tcgcagattg ccaaacgcct ttccgggatg ttcacgctgg tcgccgtact
     9541 gcccggcttg ttcctgttcg gcatttccgc gcagtttatc aacggcacga ttaattcgtg
     9601 gttcggcaac gacacccacg aagccctcga acgcagcctt aatttgagca agtccgcact
     9661 ggatttggcg gcagacaatg ccgtcagcaa cgccgttccc gtacagatag acctcatcgg
     9721 caccgcctcc ctgtcgggca atatgggcag tgtgctggaa cactacgccg gcagcggttt
     9781 tgcccagctt gccctgtaca atgccgcaag cgggaaaatc gaaaaaagca tcaatccgca
     9841 ccaattcgac cagccgcttc ccgacaaaga acattgggaa cagattcagc agaccggttc
     9901 ggttcggagt ttggaaagca taggcggcgt attgtacgcg cagggatggt tgtcggcagg
     9961 tacgcacaac gggcgcgatt acgcgctgtt cttccgccag ccgattcccg aaaatgtggc
    10021 acaggatgcc gttctgattg aaaaggcgcg ggcgaaatat gccgaattga gttacagcaa
    10081 aaaaggtttg cagacctttt ttctggtaac cctgctgatt gcctcgctgc tgtcgatttt
    10141 tcttgcgctg gtcatggcac tgtattttgc ccgccgtttc gtcgaaccca ttctgtcgct
    10201 tgccgagggc gcaaaggcgg tggcgcaggg tgatttcagc cagacgcgcc ccgtattgcg
    10261 caacgacgag ttcggacgtt tgaccaagct gttcaaccat atgaccgagc agctttccat
    10321 cgccaaagaa gcagacgaac gcaaccgccg gcgcgaggaa gccgcccgcc actacctcga
    10381 gtgcgtgttg gatgggttga ctaccggtgt ggtggtgttt gacgaaaaag gccgtttgaa
    10441 aaccttcaac aaggcggcgg aacagatttt ggggatgccg ctcgcccccc tgtggggcag
    10501 cagccggcac ggttggcacg gcgtttcggc acagcagtcc ctgcttgccg aagtgtttgc
    10561 cgccatcggt gcggcggcag gtacggacaa accggtccag gtggaatatg ccgcgccgga
    10621 cgatgccaaa atcctgctgg gcaaggcgac ggtattgccc gaagacaacg gcaacggcgt
    10681 ggtgatggtg attgacgaca tcaccgtgct gatacgcgcg caaaaagaag ccgcgtgggg
    10741 tgaagtggcg aagcggctgg cacacgaaat ccgcaatccg ctcacgccca tccagctttc
    10801 cgccgaacgg ctggcgtgga aattgggcgg gaagctggac gatcaggacg tgcaaatcct
    10861 gacgcgttcg accgacacca tcatcaaaca ggtggcggcg ttaaaagaaa tggtcgaggc
    10921 attccgcaat tacgcgcgcg ccccttcgct caaactggaa aatcaggatt tgaacgcctt
    10981 aatcggcgat gttttggccc tgtacgaagc cggcccgtgc cggtttgagg cggaacttgc
    11041 cggcgaaccg ctgatgatgg cggcggatac gaccgccatg cggcaggtgc tgcacaatat
    11101 tttcaaaaat gccgccgaag cggcggaaga agccgatatg cccgaagtca gggtaaaatc
    11161 ggaaacgggg caggacggac ggattgtcct gacggtttgc gacaacggca agggattcgg
    11221 caaggaaatg ctgcacaatg ctttcgagcc gtatgtgacg gataagccgg cgggaacggg
    11281 actgggtctg cctgtagtga aaaaaatcat tggagaacac ggcggccgca tcagcctgag
    11341 caatcaggat gcgggtgggg cgtgtgtcag aatcatcttg ccaaaaacgg tagaaactta
    11401 tgcgtagcag cgatatttta attgtagacg acgaagtcgg catccgcgac ctgctgtcgg
    11461 aaatcctgca ggacgaaggc tattcggtcg cgttggcgga aaatgccgaa gaggcgcgca
    11521 agctgcgcca tcaggcgcgc ccggcaatgg tgctgctgga tatttggatg cccgattgcg
    11581 acggtatcac ccttttgaag gagtgggcga aaaacgggca gctcaatatg ccggtggtgg
    11641 tgatgagcgg gcatgccagc atcgataccg ccgtagaagc gaccaaaatc ggcgcgatcg
    11701 attttttgga aaaaccgatt tccctgcaaa agctgctgtc tgcggtcgaa aacgcgttga
    11761 aacacggtgc ggcgcaaatc gaaacgggac cggtgttcga caaactgggc aacagtgccg
    11821 cgattcaaga gatgaaccgc gaagtcgaag cggcggcaaa gcgcacgtcc cctgtatttt
    11881 tgacgggcga ggcgggttcg ccgtttgaaa ccgtggcgcg ctatttccat aaaaacggta
    11941 cgccgtgggt cagtccggca agggtcgaat atctgatcga tatgccgatg gaactgttgc
    12001 agaaggcgga gggcggcgtt ttgtatgtcg gcgacatcgc ccaatacagc cgcaatatcc
    12061 aaaccggcat tacctttatc atcggcaagg cagaacgctg ccgtgtcagg gtgattgctt
    12121 cgtgcagcta cgcggcaggt tcagacggca tttcatgtga agaaaaattg gccgggcttt
    12181 tttcggaatc ggtcgtccgt attccgcccc tgagtatgca gcatgaagac attcccttcc
    12241 tgatacaggg gattgcctgc aatgtggcgg aaagccaaaa aatcacgcct accgcattca
    12301 gtgacgatgc gcttgccgta ttgtcccgtt acgactggcc ggggaatttc gagcagttgc
    12361 aaaacgtcgt gacaacgctg ctgctggagg cggacgggca ggaaatcggt atcggcgcgg
    12421 cttcttcggc actggggcgc ggtgtgcctg ccgacggaac ggggcatatg gcgtgcgggt
    12481 tcgactttaa cctgccgctg cgcgaattaa gggaggaggt ggagcggcgt tatttcgagt
    12541 accacatcgc ccaagaaggt cagaatatga gcaaagtggc gcagaaggtc ggtttggaac
    12601 gcacgcacct ttaccgcaaa ctcaaacagc tcggcatcgg cgtttcgcgc cgggcggggg
    12661 aaaaaaccga agaataggcc cggacggccg gtttcccggc tgcgggcttt tgttttcaga
    12721 cggcatttgg agaaaatgcc gtctggaatc gtaaggggat ggattttatg acagaggacg
    12781 aacgtttcgc gtggctgcaa ttggcgttta cgccctatat cggcgcggaa agtttcctgc
    12841 tgctgatgcg cagtttcggc agcgcgcaaa atgccttgtc cgcaccggcg gaacaggttg
    12901 ccccggcggt gcggcataag cacgcgcttg aggcttggcg caatgcggaa aaacgcgctt
    12961 tggcgcggca ggcggcagaa gcggcgttgg aatgggaaat gcgggacgga tgccgcctga
    13021 tgctgcttca ggatgaagat ttccccgaaa tgctgacgca ggggctgacc gcgccgccgg
    13081 ttttgttttt gcgcggcaac gtgcggctgc tgcacaagcc ttccgccgcc atcgtcggca
    13141 gccgccatgc cacgccgcag gcgatgcgga ttgccaaaga tttcggcagg gcgttgggtg
    13201 ggaaaggcat tcctaccgtg tcgggtatgg cttcgggcat cgacaccgcc gcccatcagg
    13261 gcgcactgga ggcagaaggc ggcaccatcg ccgtgtgggg gacgggcata gaccgcattt
    13321 atccgccggc caacaaaaac cttgcctatg aaatcgccga aaaagggctg attgtcagcg
    13381 agttccccat cggcacgcgg ccgtatgccg gcaactttcc gcgccgcaac cgcctgattg
    13441 ccgcgctgtc gcaggttacg ctggtggtcg aggccgcctt ggaatccggt tcgctgatta
    13501 ccgccggcct ggcggcagaa atggggcgcg aagtgatggc ggtacccggt tcgatagaca
    13561 acccccacag caaaggctgc cacaaactga ttaaagacgg agcgaaactg acggaatgtt
    13621 tggacgacat cctgaacgaa tgcccggggc tattgcaaaa tacgggtgct tcgtcatatt
    13681 ctataaataa ggatacgccc gacacgggcc gccgcgccgt tcagacggca tacgccccgc
    13741 cgcctgccgc aaaaatgccg tctgaagggg cggcaggcgg cacagccgtc ggcggcatac
    13801 tggataaaat gggtttcgac cccatccatc ccgacgtgct tgccggacag ttggctatgc
    13861 ctgccgcaga tttgtatgcc gcactgttgg aattggaatt ggacggcagc gttgccgcaa
    13921 tgcccggcgg cagataccag cgtatccgaa cttgaacgca ctttatatta aggaacacga
    13981 atgaccgaag tcatcgccta cctcatcgaa catttccaag atttcgatac ctgcccgccg
    14041 cccgaagact tgggtatgct gcttgaagaa gcgggtttcg atacgatgga aatcggcaac
    14101 accctgatga tgatggaagt attgctcaac agctccgaat tttccgccga acccgccggc
    14161 agcggcgcat tgcgcgtgta cagcaaagaa gaaaccgaca acctgccgca ggaagtgatg
    14221 gggctgatgc agtatctgat tgaagaaaaa gccgtcagct gcgaacagcg ggaaatcatc
    14281 atccacgcgc tcatgcacat ccccggcgac gaaattaccg tagataccgc caaagtgctg
    14341 accctgctgc ttttatgggc aaacaagagc gagctgcccg tattggtcgg cgacgagctg
    14401 atgagcgcgc ttttactcga caacaaaccc acgatgaact gaagcggctt cagacggcgc
    14461 cggcccgccc gatgccgtct gaaacaccgg cgtcaaaacc accatccaga gaacgacaaa
    14521 tggcgaaaaa cctcttaatc gtcgaatccc cgtccaaagc caaaaccctg aaaaaatatt
    14581 tgggcggcga ttttgaaatc ctcgcgtcct acggacacgt ccgcgacctc gtccccaaaa
    14641 gcggcgcggt cgatcccggc aacggctttg cgatgaaata ccaactcatc agccgcaacg
    14701 ccaaacacgt cgatgccatc gtcgccggag ccaaagaagc cgaaaacatc tacctcgcaa
    14761 ccgacccgga cagggaaggc gaagccattt cctggcatct tttggaaatc ctcaaatcca
    14821 aacgcggcct gaaaaacatc aagccgcagc gcgtcgtgtt ccacgaaatc accaaaaacg
    14881 ccgtgctcga tgcggttgcc catcctcgcg aaatcgaaat ggatttggtc gatgcgcaac
    14941 aagcccgtcg cgctctggac tatctggtcg gtttcaacct ctcgccgttg ctgtggaaaa
    15001 aaatccgacg cggtttgagc gcgggccgcg tgcaaagccc tgctttgcgc ctgatttgcg
    15061 aacgcgaaaa cgaaatccgc gcgtttgaag cgcaggaata ttggacggta catctagaca
    15121 gccacaaagg ccgcagcaag ttcaccgcca aactcgccca atacaacggc gcgaaactcg
    15181 aacaattcga cctgccggac gaagccgctc aagccgacgt gttgaaagaa ctcgaaggca
    15241 aagaggctgt cgttactgcc attgaaaaga aaaagcgcag ccgcaacccc gccgcgccgt
    15301 tcaccacatc caccatgcag caggatgccg tgcgcaaact cggcttcacc accgaccgca
    15361 ccatgcgtac cgcccagcag ctttacgaag gtatagacgt agggcagggc gccatcggtc
    15421 tgattaccta tatgcgtacc gacagcgtga atttggctga cgaagcgtta accgaaatcc
    15481 gccattacat tgaaaacaaa atcggcaaag aatatctgcc gagtgccgcc aaacaataca
    15541 aaaccaaatc caaaaacgcc caagaagccc atgaagcgat ccgcccgact tccgtgtacc
    15601 gcacgcccga aagcgtcaaa cccttcctga gtgcagacca gttcaaactc tatcaaatga
    15661 tttggcagcg taccgttgcc tgtcagatga cgcccgccaa attcgaccaa accaccgtcg
    15721 atattaccgt cggcaaaggc gtgttccgag taaccggaca agtgcaaacc ttcgcaggct
    15781 tcctcagcgt ttacgaagaa agcagcgacg atgaagagag cgaagacggc aaaaaactgc
    15841 ccgaaatgag cgaaggcgac aaattgccgg tcgacaaact ctacggcgaa caacacttca
    15901 ccaccccgcc gccgcgctac aacgaagcca cactggttaa agcccttgaa gaatacggca
    15961 tcggccgccc ctcgacctac gccagcatca tctccacgct caaagaccgc gaatacgtta
    16021 cccttgagca aaaacgcttc atgccaaccg acacaggcga catcgtcaat aaattcctga
    16081 ccgaacactt cgcccaatac gtcgattacc acttcaccgc caaactcgaa gaccagcttg
    16141 acgaaatcgc cgacggcaaa cgccgctgga ttcccgtgat ggacaaattc tggaaaccgt
    16201 tcatcaaaca agtggaagaa aaagagggca tcgaacgcgc caaatttacc acgcaggaac
    16261 ttaacgaaac ctgcccgaaa tgcggcgaac acaagctgca aatcaagttc ggcaaaatgg
    16321 gccgcttcgt cgcctgcgcc ggctatcccg aatgcagcta cacgcgcaac gtcaacgaaa
    16381 ccgccgaaga agccgccgaa cgcatcgcca aagcggaagc cgaacaggtc gaactcgacg
    16441 gtcgcgaatg ccccaaatgc ggcggacggc tggtgtacaa atacagccgc accggcagta
    16501 aattcatcgg ttgcgccaac taccccaaat gcaaacacgt cgagccgttg gaaaaaccca
    16561 aagataccgg cgtccaatgc ccgcaatgca aaaaaggtaa cctcgtcgag cgcaaatccc
    16621 gctacggcaa actgttttac agttgcagca cctatcccga ctgcaactac gccacttgga
    16681 acccgcccgt tgccgaagaa tgcccgaact gccattggcc ggtcttgacc atcaaaacca
    16741 ctaaacgccg gggtgtggaa aaagtctgcc cgcaaaaaga atgcggctgg aaagaacaaa
    16801 tcgaaccgcc tgcaccgcaa gagtgaagcg gttgggatta agatgaaaga aaatgccgtc
    16861 tgaaaggggt ttcagacggc atttgtaaat tagaaggggc tgtcccagat ggctggggaa
    16921 atttaaatta agtcagaatt atccctatga gaaaagccgt ccaagccggt acaaacaaaa
    16981 gaaacccatc ggactgtttg ccgcaggggt aactgcaaga acggcagcag ggttggcagc
    17041 agttaatcaa aacacggcag cctgttattt tctatggcga aatagtgttt ggcacatcat
    17101 cgggaaaact tcctttacac gcatttggac gaaatctgcg aaattatgga ggcatacgac
    17161 atatcgttta cgattactta tccgtcaaaa cagtccgcat ttggaaatgt atgacggcga
    17221 agcaggggcg ggtgaaagtt atttccgtaa aggcagacgc ggccgcagtg ccgccggcaa
    17281 agccgccgta ttcgggcttt tgaagcgcaa cggcaaggtt tgtacggtta ctgtcggaca
    17341 ctcgaaccgc tattttattg cctattatcc gcggacgggt gaaacccggc agcattgttt
    17401 atacggattg ttcgtagtta cgatgtatca gattgtaagc ggatttggcc gtttccgtat
    17461 caatcgcggc acacattttg ccgaacggca aaaccatatc aacgcaattg ggaacttttg
    17521 gaatcgggca aacgtcattt gcgcaagttt gacggcattc ccaaagagca tttcgggctg
    17581 tatttgaagg agtgcggacg gcgttttgac aacagtgggg taaaagttcg aatttccatt
    17641 ttaaaacaat tggtaaagca ggatttatcc cggttggccg ggattttaaa aaata
//
LOCUS       DAXFWM010000049        17248 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000049 DAXFWM010000000
VERSION     DAXFWM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17248
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.049"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..183
                     /locus_tag="ACFI8B_001497"
     CDS             <1..183
                     /locus_tag="ACFI8B_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834687.1"
                     /translation="SDGNPTANYFFEIIDDGYMDWLKEESFGFFEKKYYKAYIFFFSD
                     SVIEVISSTEPVFYSK"
     gene            495..1628
                     /locus_tag="ACFI8B_001498"
     CDS             495..1628
                     /locus_tag="ACFI8B_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834688.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAQWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            1635..1955
                     /locus_tag="ACFI8B_001499"
     CDS             1635..1955
                     /locus_tag="ACFI8B_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834689.1"
                     /translation="MPSETGFQTALESQTLFAMPYMPNPVRYKLKTTIQKTEIHIIKQ
                     TNKLTYCAILRAVPYFPVLYMSGRHPHKRRDTDKEKSLGIKNILINQYKSNKQFIIFF
                     SVGV"
     gene            complement(2131..2406)
                     /locus_tag="ACFI8B_001500"
     CDS             complement(2131..2406)
                     /locus_tag="ACFI8B_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC3834690.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMLSET"
     gene            complement(2403..3686)
                     /locus_tag="ACFI8B_001501"
     CDS             complement(2403..3686)
                     /locus_tag="ACFI8B_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3834691.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFMRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(3835..5253)
                     /gene="glnA"
                     /locus_tag="ACFI8B_001502"
     CDS             complement(3835..5253)
                     /gene="glnA"
                     /locus_tag="ACFI8B_001502"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC3834692.1"
                     /translation="MSIKNAVKLIEKSKARFVDLRFTDTKGKQHHFTVPARIVLEGPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            5564..6373
                     /gene="aroE"
                     /locus_tag="ACFI8B_001503"
     CDS             5564..6373
                     /gene="aroE"
                     /locus_tag="ACFI8B_001503"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC3834693.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            6376..7077
                     /gene="mtgA"
                     /locus_tag="ACFI8B_001504"
     CDS             6376..7077
                     /gene="mtgA"
                     /locus_tag="ACFI8B_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC3834694.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(7351..7426)
                     /locus_tag="ACFI8B_001505"
     tRNA            complement(7351..7426)
                     /locus_tag="ACFI8B_001505"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7391..7393),aa:Phe,seq:gaa)
     gene            complement(7617..8351)
                     /gene="lptB"
                     /locus_tag="ACFI8B_001506"
     CDS             complement(7617..8351)
                     /gene="lptB"
                     /locus_tag="ACFI8B_001506"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC3834695.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(8422..8952)
                     /gene="lptA"
                     /locus_tag="ACFI8B_001507"
     CDS             complement(8422..8952)
                     /gene="lptA"
                     /locus_tag="ACFI8B_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC3834696.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(8933..9514)
                     /gene="lptC"
                     /locus_tag="ACFI8B_001508"
     CDS             complement(8933..9514)
                     /gene="lptC"
                     /locus_tag="ACFI8B_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC3834697.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(9511..10047)
                     /locus_tag="ACFI8B_001509"
     CDS             complement(9511..10047)
                     /locus_tag="ACFI8B_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC3834698.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(10113..11087)
                     /locus_tag="ACFI8B_001510"
     CDS             complement(10113..11087)
                     /locus_tag="ACFI8B_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC3834699.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            11184..12239
                     /gene="tal"
                     /locus_tag="ACFI8B_001511"
     CDS             11184..12239
                     /gene="tal"
                     /locus_tag="ACFI8B_001511"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC3834700.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            12251..12385
                     /locus_tag="ACFI8B_001512"
     CDS             12251..12385
                     /locus_tag="ACFI8B_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834701.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            12390..12680
                     /locus_tag="ACFI8B_001513"
     CDS             12390..12680
                     /locus_tag="ACFI8B_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834702.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            12697..13584
                     /gene="gluQRS"
                     /locus_tag="ACFI8B_001514"
     CDS             12697..13584
                     /gene="gluQRS"
                     /locus_tag="ACFI8B_001514"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC3834703.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(13654..14670)
                     /gene="dusA"
                     /locus_tag="ACFI8B_001515"
     CDS             complement(13654..14670)
                     /gene="dusA"
                     /locus_tag="ACFI8B_001515"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC3834704.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            14790..15944
                     /locus_tag="ACFI8B_001516"
     CDS             14790..15944
                     /locus_tag="ACFI8B_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3834705.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKTGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(15994..16260)
                     /locus_tag="ACFI8B_001517"
     CDS             complement(15994..16260)
                     /locus_tag="ACFI8B_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFC3834706.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(16368..>17248)
                     /locus_tag="ACFI8B_001518"
     CDS             complement(16368..>17248)
                     /locus_tag="ACFI8B_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC3834707.1"
                     /translation="LRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
BASE COUNT         3961 a         4598 c         4645 g         4044 t
ORIGIN      
        1 tcggatggga atcctactgc aaattatttt ttcgagataa tagacgacgg ttatatggac
       61 tggttgaaag aagaaagttt tggttttttt gaaaagaaat attataaggc ttatattttc
      121 ttttttagcg attctgtaat agaagttatc agctcgacag aacctgtatt ttattcaaaa
      181 taacaaatta tcaaacaaag ctctgattaa aaacccaaca atcaaaatgc catctgaagc
      241 ggtattcggc tttcagacgg cattttctcc gattagtaca ggatgcaggg gcttgccggc
      301 ttgattttgc cgaagttgtt ggcacgcaat ttggaggggg cgacaaccta tgaaaaataa
      361 atcgttaagg atatttttag ttttacccaa tttgtttccg aaaaagtacg gcagcaaccg
      421 tagattagat taaaaatcaa acaatcaaag cccgcctgat ataatcgata tatcctgtta
      481 aacgaaaaat aaatatgttt aaaaaattca aaccggtatt gttgtcattt tttgcacttg
      541 tatttgcctt ttggctggga acgggcattg cctatgagat taatccgcgt tggtttttga
      601 gcgatacggc aactgaaata cctgaaaatc cgaatacttt tgtggcgaaa cttgcccgcc
      661 tgttccgaaa tgccgacagg gcggttgtca tcgtgaagga atcgatgagg acggaggaaa
      721 gccttgccga aactgtggat gacggtccgt tgcagtcgga gaaggattat ctcgcgctcg
      781 ctatccggct cagtcgtttg aaagaaaagg cgcaatggtt tcacgtaacg gagcaggaac
      841 atggggaaga ggtctggctg gattactata tcggcgaggg cggtttggtt gcggtttcgc
      901 ttttgcaacg atcgccggaa gcgtttgtta atgccgaata tctgtatcgg aacgatcgtc
      961 cgttttctgt aaatgtgtac ggcggaacgg ctcacgggga aaattatgaa acgacaggag
     1021 aatatcgggt tgtttggcaa ccggacggtt cggtatttga tgcgtcgggg cgcgggaaaa
     1081 tcggggaaga tgtttatgag cattgcctcg ggtgttatca gatggcccag gtatatttgg
     1141 cgaaataccg ggatgtcgcg aatgacgagc agaaggtttg ggacttccgc gaagagagca
     1201 accggattgc gtcggactcg cgcgattatg tgttttatca gaatatgcgg gaattgatgc
     1261 cccgggggat gaaggcgaac agtcttgtgg tcggctatga tgcggacggt ctgccgcaaa
     1321 aagtctattg gagtttcgac aatggaaaaa aacgccagag tttcgaatat tatttgaaaa
     1381 acggaaatct ttttattgca caatcttcga cggtagcatt gaaagcggat ggcgtaacgg
     1441 cggatatgca gacctatcat gcgcaacaga cgtggtattt ggatggcggg cggattatcc
     1501 gcgaagagaa acagggagac agactgcctg attttccttt gaacttggaa gatttggaaa
     1561 aagaggtgag ccgttatgca gaggctgcgg cgagacgttc gggcggcagg cgcgaccttt
     1621 ctcactgaac gcaaatgccg tctgaaaccg gttttcagac ggcattggag agtcaaacgc
     1681 tttttgcaat gccgtatatg ccgaacccgg ttcgctacaa attgaaaacc acaatccaaa
     1741 aaacggaaat acacattatc aaacaaacaa ataagttgac atattgtgcg atattgaggg
     1801 ctgtaccgta ttttcccgtt ctttatatga gcggcaggca tcctcataaa agaagggata
     1861 ccgataagga aaaatccctc ggtataaaaa atatactgat taatcaatac aaatccaaca
     1921 aacagttcat cattttcttt tctgttggcg tataaaaacc gctcggaaaa aaataaaata
     1981 tcaaaacggc aaaataaaaa tactgatata tgccttcact ttcatagggc gacgctctat
     2041 gtgccgtcct gtgtgttgaa acgtcggttt tccttaccgc ctgccccgct tttccctgat
     2101 tgctgccaac tgccgtccga acctgaaccg tcaggtttca gacagcattt tttctccctg
     2161 ttcccacaat tgccgttttt gtgcggcggt cagttttttg acggcgattt cctgtttgag
     2221 cgcagtgcct ttatccatgc cgcctgcaac gatacgcatc gccaccggtt tgaatacgcg
     2281 ggtatatttc gcgccttttc cggctgtgtg gatggcaagc cgctgttgcg gattcgggct
     2341 gataccgcaa tagaacgcgc tgttttcaca caatatcagg taaacgctcc agtttgacgc
     2401 gttcatctgc ctgcatcctg ggttttttcc ccgttccaag gcgcaacttt cagcagcagc
     2461 agcatacccg gcagggcaag tatgaaacac agccggaaaa acggtacata acccatccat
     2521 tcgatcagat agcctgcaaa ggaattgatg accgtgcgcg gcacggcgga caggctggta
     2581 aacagcgcaa gctgcgttgc cgtaaacgcg ggattggtct cgcgcgccat atacgatacg
     2641 aacgccgccg tccccaaccc cacgccgacc gcttccgcgc cgataactgc cgccagcatc
     2701 agcctctcgc ctgtgccgac cgtgtcgaaa tgtccgaacc ctgccagcca tacaaacccc
     2761 aaaaccgtta ttgcctgcac cgcgccgaat agccacaagg ctttgtttac gccgattttc
     2821 agcatccaca cgccgcccaa gatacctgcc gccactgccg gccacagtcc tgcatttttg
     2881 gcaatcaaac cgatgtcggt cttgctgaaa cccatatcca gataaaacgg cgttgccaac
     2941 gcggttgcca tactgtcgcc gagtttgtaa aggaagataa acagcagcac gcacaccgcc
     3001 gaagcgatgc ccttgcgcat aaaaaattct ttaaacggct ctaccacggt ctgcttcaac
     3061 gttttaggaa cggaaggcgg caacacgggt tcgtgcgcga gaaacagcgt catcagaagg
     3121 ccgggcagca taaataatga agtgataaca aatacttctg accacggcat cctgtctgcc
     3181 aacaccaaac tcaatgaacc gggaatcagg gcggcaaccc ggtaggcgtt cacatgaacc
     3241 gagttgccca aacccaattc ttcgtccgac aaaatctcgc gcctgaacgc atccaataca
     3301 atgtcctgac tggcggaaaa aaaagcgaca agcaccgaca agccggcaat caacggcaga
     3361 tgattccggg ggtttaaaaa ggcatatgcc gccaaagccg ccagtaaccc tgcctgcgtc
     3421 agcagcatcc acccgcgccg ccgtcccaaa acgggcagcc tgaccgcgtc catcagcggc
     3481 gaccacaaaa atttccaagt aaacggcaga ccgatcaacg ccatcagccc gatgctcttc
     3541 aaatccacct gctcgctgcg caaccacgcc ggaatcaggt taatcagaaa gtacagcggc
     3601 agccccgagg caaaccccgt aaaaatacag acgagcatat tacgggaaaa gatttgcccg
     3661 ataaaacctg atttcgatgc agtcatcgtt cgtacagcca aaaaacaaaa gcctattgta
     3721 gcaaatatcg cccgcctgct gaaaaatgcc gccacgcgcc gccggtttgc cgttccaatg
     3781 caaaatgccg tctgaaactg tgttcagacg gcattgctac tgaaacccga cggtttacag
     3841 gctgtaatac atttcaaatt ccagcgggtg cggcgccata cggatgcggc ggacatcttc
     3901 ctctttaaag gcgatgtagc tgtcgatcca gtctttgctg aacacgccgc cgcgcagcag
     3961 gaattcgtgg tcggccttga gggcggcaag tgcttcttcc aaagaagcgc agacggtcgg
     4021 gacgagcgcg tcttcttccg gcggcaggtc gtacaggttt ttatcggcag ggtcgcccgg
     4081 atggattttg ttttgaatgc cgtccaaacc cgccatcagc agggcggcaa atgccaaata
     4141 cgggttggcg gtcgggtcgg ggaaacgcgc ttcgatgcgg cgcgccttgc tgctgttcac
     4201 agacgggata cggatggaag cggaacggtt tttggcggaa taggccaatt tggtcggtgc
     4261 ttcaaagtgc ggcacgaggc gtttgtagga gttggtggac ggattggtaa tcgcgttcag
     4321 ggctttggcg tgtttgatga tgccgccgat gtagtagagc gcggtatcgg acaaaccggc
     4381 atagccgtcg cctgcgaaca ggttttgacc gtctttccaa atggattggt ggacgtgcat
     4441 accgctgccg ttgtcgccca taatcggttt gggcataaag gtggcggttt tgccgaaatt
     4501 gtgggcaacg ttttggatga cgtatttcat atcttgggtt tggtcggcgc gtttgaccaa
     4561 agtggcgaaa cgggtgccga tttccatttg gctgccggta ccgacttcgc tgtggtggac
     4621 ttcgacttcg atgccgagtc cttccaaaat gttcaccatg gcggagcgca aatcttgacc
     4681 gcagtcaatc ggcgcgacgg gcgcgtagcc gcctttgacg gcggggcggt ggccggtgtt
     4741 ttgaccgtcc atatgcaggc cgcttgccca cgcgccgctt tcggacgtga tttcgtaacg
     4801 ggttttgtgc atgtcggttt caaattctac gccgtcgaag acgaagaatt cgggttcggg
     4861 gccgaaatag gcggtgtcgc cgataccgga agatttcaaa taggcttcgg cgcggcgtgc
     4921 gatggagcgc gggtcgcggt cgtaaccctg accgtcggca gggtcgatga cgtcgcaggt
     4981 aatgacgacg gtaacatcat cataaaaagg atcgacgaag gctgtggacg catcgggacg
     5041 cagctgcata tcggaagcct gaatgccttt ccagccgccg atggacgaac cgtcaaacgc
     5101 ctgaccgttt tcgaaccact cttcggggcc ttccaacacg atgcgcgcag gcacggtaaa
     5161 gtggtgctgc ttgcctttgg tatcggtgaa gcgcaaatcg acaaagcggg ctttgctttt
     5221 ttcaatcaat tttacggcgt ttttaatgga catcttcagc tcctgaaaaa gggaaatggc
     5281 aaaatacgcg gaacgcggcg gcttgacgca aagtcggcac gattttacca cgccgatgcc
     5341 gtctgaaacc ggttttgcgt caaattccaa gccggaaaat atagtttcaa acggcaagat
     5401 tgtcaacaat atgcttcggt ttgtctgttc aaaagcggaa tgcccgccct tttgtccgaa
     5461 tgccgacaaa acagatgccg tctgaaggcg gtttcagacg gcatcgttcc catcttcgcg
     5521 ccgccgttat aaaatgaccg ccccgccttt atggaatatg actatgcacg ccctcccccg
     5581 ctacgccgtt ttcggcaacc ccgccgccca cagcaaatcg ccgcagattc atcagcaatt
     5641 tgcccttcag gaaggcgttg acatcgaata cggacgcatt tgcgccgaca tcggcggttt
     5701 cgcgcaggcg gtttcgacat tttttgaaac aggcggttgc ggggcaaacg ttaccgtacc
     5761 gttcaaacag gaagcgtttc atctggcgga cgaacattcc gaccgcgctt tggctgccgg
     5821 tgcggtcaat acgctggtgt ggttggaaga cggcagaata cgcggcgaca ataccgacgg
     5881 tatcggtttg gccaacgaca tcacgcaggt caaaaacatt gccatcgagg gcaaaaccat
     5941 cttgcttttg ggcgcgggcg gcgcggtgcg cggcgtgatt ccggttttga aagaacaccg
     6001 ccctgcccgt atcgtcattg ccaaccgtac ccgcgccaaa gccgaggaat tggcgcggct
     6061 tttcggcatt gaagccgtcc cgatggcgga tgtgaacggc ggtttcgata tcatcatcaa
     6121 cggcacgtcc ggcggcttga gcggtcagct tcctgccgtc agccctaaaa ttttccgcga
     6181 ctgccgcctt gcctacgata tggtttacgg cgaagcggca aaaccgtttt tagattttgc
     6241 acgacaatcg ggggcgaaaa aaactgccga cggactgggt atgctagtcg gtcaggcggc
     6301 ggcttcctac gccctctggc gcggtttcaa accggacatc cgccccgtca tcgaacacat
     6361 gaaagccctg taaccatgtt ccgcatcgtc aaatggctga ttgccctgcc cgtcggcatc
     6421 tttatcttct tcaatgccta tgtgtacggc aacatcatta cctaccgcgc cgtcgcgccc
     6481 catcggactg cctttatgtc gatgcggatg aagcagtttg aacaagaagg tcgcgatgtc
     6541 gcactggatt accgctgggt gccctacaac cgcatttcca ccaacctgaa aaaagccctg
     6601 attgcttccg aagatgtccg ttttgccgga cacggcggct tcgattgggg cggcattcaa
     6661 aacgccatca ggcgcaaccg gaacagcggc gaagtgaagg cgggcggatc gaccatcagc
     6721 cagcagcttg ccaaaaacct cttcctcaac gaaagccgca actatctgcg caaaggggaa
     6781 gaggcggcca ttacggcaat gatggaagct gttaccgaca aaaacaggat tttcgaactg
     6841 tatttaaact caatcgaatg gcactacggc gttttcggcg cggaagctgc gtcccggtat
     6901 ttttataaaa aaccggccgc agacctgacc aaacagcagg cggcgaaact gacggcactc
     6961 gtccccgccc cgctctacta cgctgaccat ccgaaaagca aacggctgcg caacaaaacc
     7021 aatatcgtgc tcagacgcat gggttcggca gaattacccg aaagcgatac ggactgattg
     7081 tttcagatat ggaaatgccg tctgaactgc ggttcggacg gcatatgttt tctggttctt
     7141 atagtggatt aaatttaaac cggtacggcg ttaccccgcc ttgccgtact atgttgtact
     7201 gtctgcggct tcgtcgcttt gtcctgattt ttgttaatcc actaacctgc ccgacagccg
     7261 caaagaccgt atcgtgcatg gcaggtattt gcggtgcggg actgctgccg aaaacggcag
     7321 gcaaaaaata accgcttata agcggttttt tggtggccag aggcggaatc gaaccgccga
     7381 cacacggatt ttcaatccgt tgctctacca actgagctat ctggcctgta tctgtctcgt
     7441 taagaggtgc gtattaaacc aaatttcggg acgacacgca agcggatttg tcgatatttt
     7501 ttacccaacc gactaagtcg atgttttaag tttaaattaa tttcacagaa aaatagtgca
     7561 aatcggtttt ttgccgttcg ttcggcgatg ttctgctttg cagacggcaa aacgcttcaa
     7621 tatttgaagt tctcgcccaa atagacggcg cgcacctgtt cgttgccgac caaatcgtcg
     7681 ggcttgccgg aagccagcac cgcaccgtct gaaatgatgt aggcgcggtc gcagatgctg
     7741 agggtctcgc ggacgttgtg gtcggtaatc aatacgccga tgccgcgcga tttgaggaaa
     7801 ccgatgattt tctggatgtc gatgacggcg atgggatcga cgccggcaaa aggttcgtcc
     7861 aaaaggatga aacgtggttt catggcgagg acacgggcga tttcgacgcg ccgccgttcg
     7921 ccgccggaca gcgacggcgc aggattgcgg cgcaagtgtc cgatattgag gtcggcgagc
     7981 agtttttcga tttctctgtc gatttgattt ttatctttgg ttctgatttc caagatggcg
     8041 cggatgtttt gttcgacggt cattttgcgg aatatcgagg cttcctgcgg caggtagccg
     8101 acacccaggc gggcgcgttc gtgtatgggc aggtggcgca attcccgccc gtccagcatc
     8161 acgctgcctg cgtcggcagc gatgagcccg actatcatgt agaagccggt ggttttgcct
     8221 gcgccgttgg gcccgagcag tccgatgact tcgccgcttt cgatctcaag ggagaaactt
     8281 ttgacgactt ggcgtttttt gaaacttttc tgcaggtttt ggacgacgag gcggctgttg
     8341 tttgcgctca taaatctctt ctcggcattt cggtcggcaa atgccgtctg aactgcgttt
     8401 ccgtttcaga cggcatcggg gttattcggt tttttgtgtg cttgaaggct ggatgacgac
     8461 gctgaccctg ccggttttgg aagcggattt cgcacccgat ttcgtgctgc cgttgatggt
     8521 atagacttcg gttttggtgt tgtaggtaat gaccgcacct tcggcaacgt cgccgccgcg
     8581 ctgcactttg gcattgccgg tcagaacgac ggtgcttcct gcggaggaat aggtaacgtt
     8641 gtttgcctga ccgcgcaccg tccctttgcc cccgtccaac gtttggctga agcggacggg
     8701 cgaaccttcc gccctcacgg attcgccgcc tttgccgccg cgtgtgacgt tgacgcgcga
     8761 ggcggaaatg ttgagcgtac cctgtctgat gatgacattg ccgctaaatg tggtactttg
     8821 gttggcttga tcgagcgaac cttggtcggc ttcgatttgg atgggccgtc tgctgtcgct
     8881 ttgaagggca aaagcgggag aagttgcaaa aattacaatt aaaacaaata gcttacatat
     8941 cttttgtatc ataaatcgcg gctttcactt tagatgagaa gttcaacatg cctgttttgt
     9001 ggttgtaggt cataccgccc gcctgaccgt gcgacgcacc atattggaaa ctgacaggcg
     9061 tatcggtttg ggcatattga gattcggtat cgacgtgcag tttttcggtt tcgactttac
     9121 ccgcctgccg cctgccgtcg gcggttttgg tcagcacaac gttgttttta aaaagaacct
     9181 gtttgttttc ggtatggtaa acggcttcat cgctgccgac ttcgtacaac agcctgcctt
     9241 cttggaagaa cacgagatgc ggcgaatcaa aatggatgtc gctgttttcg ggaaactgtt
     9301 tcgcaccttt cgcgctcaaa tgttctttca agtatccctg ttcgtcaaac cgccttccgt
     9361 ccaagccgtc cattgtgtat tgaggttcgt cgggattgag cctgacttcc tcgatttcga
     9421 cttcgctgat acggcccaac catgccgaca ggctgcccaa ggcaaccgcc aatatcaatg
     9481 ggaacgcaat tccgtaccgc catcttactt tcatttgatg tacccgttca aagccgcgcc
     9541 caacgtacct ttcgcgcgga taatcaggtc acacacttcg cgcaccgcgc ccgcaccgcc
     9601 ggggcgtttg gcaatatagg cggcgtgttg cagcgtaaac caatgcgcgt cggggacggc
     9661 aaccggcaat ccgcagcgca ccatcaccgg caaatcgacc acgtcgtcgc cgacaaaggc
     9721 gcactcggct tcttccaagc ctgcctgcgc gcgcagctct tcatacgcgg cgcgtttgtc
     9781 cgaaatgcct ttgaagtagt aatttatgcc caactgtttg acgcggatgc cgacggaggg
     9841 cgcatcccga cctgtgataa tcgcagtctg cacgccgctt gcctgaagca ttttcaggcc
     9901 gtgtccgtcc agtgtgtgaa acgatttgat ttcttcgccg tcgtcgcgga taaagatgcg
     9961 cccgtcggtc aaaacgccgt ccacatccaa tatcagcagt ttgattttgg cggcgcgtgc
    10021 ctgtaattcg ggagaaattg cctgcattgg gatttccttg ttttcagacg gcatatctta
    10081 ccacaaatgc cgtctgaaac ggctgcaacg ccttatacaa tccgcgccgc caaaaggtcg
    10141 tgcatattca gcgcacctgt cagcacgccg tctgcatcgg taaccagaag cccgttcaca
    10201 tggtttgcct gcatgacttt cagggcttcg gtggcgaggc gttcggcgga gatggtttta
    10261 gggtgtgtat gcatgacttc gtctatcgaa agaccggtaa aattgtcgca ttcttgaaac
    10321 aggcggcgca aatcgccgtc agtaaacacg cctttcagac agccttgccc gtccgttacc
    10381 gccaacatgc ccaaaccttt ctcgctcatg ctgacgatgg ctcctttcaa gggcgtgccg
    10441 agtcggacgg cgggcaggcc gccgcctttg tgcataatgt cggcaacgcg caaaagcagg
    10501 cgtttgccga ggctgccggc agggtggatc aaggcgaagt cgtcgggcgt gaacgcgcgg
    10561 gcgcgcagca ggacgaccgc caacgcgtcg cccaaagcca taacggcggt ggtgctggtg
    10621 gtcggggcaa gccccaacgg gcaggcttct tgcgaaaccg atgcggtgat gtggatatcg
    10681 gcatggcgcg ccatggttga atcggggcgg gcggtgatgc agacgagggt aatgtctttg
    10741 cgtttgagcg cggggatgat ggcggtgatt tcgtcgcttt cgccggaatt ggaaatggcg
    10801 gcgacgacgt cgttgtccac aatcatgccc aaatcgccgt gtgccgcttc cgcagggtgg
    10861 acgaaaaacg cgggcgtgcc ggtcgaggcc atggttgccg ccattttgcg cccgatatgt
    10921 ccggacttgc ccatacccgt gatgacgacc ctgcccttgc agtgcaacaa cgcgtctgcc
    10981 gcaaggacga agttttcgtc caattccgcc gcaatttcgc gcaagccttc cgcttcggtg
    11041 tgcaacactt cgcgcgccca gtcgagatat ttttcgtttt ctgccattgc catccgcctt
    11101 tgctgtaaca aaactaccca aaatttccga atcggggtag cataagcctt caaaccccgc
    11161 ccatccatcc aaaggaatca aaaatgacta ttttatcgga cgttaaagca ttaggacaac
    11221 aaatctggct ggacaacctt tcccgctcgc tcgtgcaaag cggcgaattg gcgcaaatgc
    11281 tcaaacaagg cgtgtgcggc gtaacttcca atcccgccat tttccaaaaa gccttcgccg
    11341 gcgacgcgct ttatgccgac gaggtcgccg ccctcaagcg gcaaaacctc agccccaaac
    11401 aacgctacga aaccatggcg gttgccgacg tacgggcagc ctgcgacgtt tgccttgccg
    11461 aacacgaatc caccggcggc aaaaccggtt tcgtcagtct cgaagtttct cccgaacttg
    11521 ccaaagacgc tcaaggcacg gtagaagaag cccgccgcct ccatgccgcc atcgcgcgta
    11581 aaaacgccat gattaaagtg cctgccaccg acgcaggcat cgatgcgctc gaaaccctcg
    11641 tttcagacgg catcagcgtg aacctgaccc tgctgttctc acgcgcccaa accctcaaag
    11701 cctacgccgc ctacgcgcgc ggcattgcca aacgcttggc agccggacaa agcgttgccc
    11761 atatccaagt tgtcgccagc ttcttcatct cgcgcgtgga cagtgcgctg gatgcaacgc
    11821 tgcccgaccg gctcaaaggc aaaaccgcca tcgcccttgc caaagccgcc tatcaagatt
    11881 gggaacaata tttcaccgcc cccgaatttg ccgcactgga agcccaaggc gcaaaccgcg
    11941 tgcagctttt atgggcatct accggcgtga aaaacccagc ctatcccgac acgctctacg
    12001 ttgacagcct gatcggcgtg cacaccgtca acaccgtccc cgatgccacg ctcaaagcct
    12061 ttatcgacca cggcacggcg aaagcgacgc tgaccgaaag cgcggacgaa gcacgggcgc
    12121 ggctcgccga aattgccgcg ctcggcatcg atgtcgaaac cttggcggcg cgtttgcagg
    12181 aagacggttt gaaacagttt gaagaagcct ttgaaaaact gctcgcgcct ttggtttagg
    12241 catttccccg atgccgtctg aaaggctttc agacggcatc gcccttttga ccggacacag
    12301 tgcggcatat caaggatccg ccctaaaaat attaatgggc aagaagttaa tgcacgacat
    12361 tacccgccaa cacaaaagga aatagaatca tggcaaaagc actcgaaatc atttcacccg
    12421 atgaaattta ttcagatctg atttttaagg atccggtacc tccccatact atatatacag
    12481 aattgatgaa actggttggt agaaatgcag gcaatgagag aattgcagat agagccttcg
    12541 attttttctc gcctgctctt atggatgatt cagctaccga agagcaatat aatgccctat
    12601 atgatttaac cttgcttgaa gagcccggga tggaactgga taaagatgag ataacggctt
    12661 tgattaatag tcttaaataa aggggctgta ccggatatgt acacaggacg cttcgccccc
    12721 agcccgaccg ggttgctcca catcggctct cttctgaccg ccgtcgcttc ctatgccgat
    12781 gcacgttcaa acggcggcaa atggctggtc cgtatggaag acctcgatcc gccgcgcgaa
    12841 atgccggggg cggcaagcca tatcctgcac acgctcgagg cattcggatt cgagtgggac
    12901 ggcgaagtca cctatcagag ccgccgttac gccctgtatg aagaaaccct ataccgtctg
    12961 aaaaccgccg gactggtcta tccctgccat tgcaaccgca aagactggca ggccggggca
    13021 aggcggggca cagacgggtt cgtctataac ggacgctgcc gccaccccgg ccaacgccct
    13081 gcactgcaag gcaaacagcc gtcgtggcgc atccgcgtcc ccgaccgcga catcggcttt
    13141 tcagacggca tcgtcggcag ttacacccaa aacctcgccc gcgacatcgg cgatttcgtc
    13201 ctgttccgtg cagacggtta ctgggcatac cagctcgccg tcgttgccga cgatgccgaa
    13261 cagggcgtta cccacatcgt gcgcggacaa gacctgctcg tttccacacc gcgccaaatc
    13321 tatttgcagc agtgtttggg cgttccgaca ccgcaatatg cccacctgcc gctgctgacc
    13381 aacgcgcaag ggcagaaatg gtcgaagcag acgctcgccc ccgcattgga ttcaaaccgc
    13441 cgcgaacaac ttctccgcca agtgttccgc tacctcaagc tgcccgaagc accggaaacc
    13501 gaccgccctg ccgaattgct cgactgggcg gtggcacact gggatatgga caaagtgccg
    13561 aaacacgcca ttaccgcccc ctaaacacca cccgacaaac gcacaaatgc cgtctgaaac
    13621 cttcagacgg catttcacac atacagcccc gccctattcg cgcgtatttg cccgttcgac
    13681 ttctttccac gcttcgaaaa tcaggccgcc gtcgttgtct ttcaagagcg ttgcatcgga
    13741 aagcatacgc cgccaagtcc gcgcgccttt caaaccgtgc atcagcccga ggctgtggcg
    13801 gacgatgtgg cgcaggattg tgccgcgtcc ggcttggatt tggacttggc tgtatgtgta
    13861 gaggcgctgc accaaatcgg cgtattcgat cggggcgcgg ttgtcaccgt aaaacagcct
    13921 gtcccattcg cgcatcacca tcgggttgtg gtatgcctcg cgcccgacca tcacgccgtc
    13981 aacgtgttgc aagtgtccgg cgattgcttc gttggtggtg atgccgccgt tgatgatgat
    14041 ttccagctcg ggaaactcct gcttgaggcg gtaaacgtaa tcgtatttca agggcggaac
    14101 atcgcggttt tctttgggcg aaagaccgtc cagccaagcg ttgcgggcgt ggacgatgaa
    14161 ggttttgcag gcggttttgt cgcgcagcgt gccgacgaaa tcggcaacgg tttggtattc
    14221 ggtctgcctg tccacaccga tgcggtgttt gacggtaacg gggatgccga ccgcgtcgtg
    14281 catggcgttg aggcagtcgg caaccagccc gacttcgttc atcagacacg cgccgaacgc
    14341 gcctttctgc acgcgcggac tgggacagcc gcagttgagg ttgacctcgt tgtagccgta
    14401 ttcttcggcg gctttggcgg ctttcgccaa atcggacgga tcgctgccgc ccagttgcag
    14461 ggcgacgggc tgctcgcctt cgttaaacat caaaaaacgg tctttgtcgc cgtaaacaat
    14521 cgcgccggcg ttgaccattt cgctgtacag ccaagcattt cgggtaatct ggcgggcaag
    14581 gtaacggtag tgcctgtccg tccagtcgag catcggggcg acgcataaac gccttttccc
    14641 tttatgttca ataagttcct tgtttatcat aaggttttca cattctttta actcaaacat
    14701 tctttattat ttgcttggat ttttgcccat ttcactatat ttgacgtacc atacttgcac
    14761 cataattttt aattcttgga atggtgcaaa tggcaacaat aaccaagcgg cgcaatcctt
    14821 ccggcgaaac agtatatcgg gttcaggtac gggtcggcaa gaaaggctat cctgctttca
    14881 atgagagcag gacgttcagt aaaaaggctt tggcggtcga atgggggaag aaacgggagg
    14941 cggaaatcga agccggtcca gaactgcttt tcaagcgcgg caaggtcaag atgatgacgc
    15001 tgtccgaagc tatgcggaag tatcttaacg aaacgcttgg agcgggtcgg tcaaagaaaa
    15061 cgggcttgcg ttttctgatg gagttcccga ttggcggcat cggcatcgat aagctgaaac
    15121 ggtctgattt cgcggaacac gttatgcaga ggcggcgcgg aatccccgaa ctggacattg
    15181 cgccaatcgc ggcttctacg gcattgcagg agctgcaata tatccgttcc gtgctaaaac
    15241 acgcctttta tgtgtggggg cttgaaatag gctggcagga attggatttc gcggcaaacg
    15301 ggctgaaacg ctcgaatatg gttgcgaaat ctgcaatcag ggacagattg ccgaccacgg
    15361 aagaactgca aaccctgaca acttatttcc tgcggcaatg gcaaagcagg aaatcttcca
    15421 taccgatgca cctgattatg tggctggcga tttatacgtc aaggcggcag gatgagattt
    15481 gccgcctgct gtttgacgac tggcacaaaa atgattgtac ccggccggtt cgtgatttaa
    15541 aaaatccgaa cggcagcaca gggaataata aggagtttga tattctgcct atggctttgc
    15601 cggtcattga cgagctgccg gaggaatcgg tcaggaagcg tatgcttgcc aacaaaggca
    15661 tcgccgacag ccttgtaccg tgcaacggaa aatcggtttc cgccgcttgg acgagggcgt
    15721 gcaaggttct tagaattaaa gacttgcgct tccacgattt acggcacgag gctgctaccc
    15781 gtatggctga agacggcttc acgattccgc aaatgcaacg ggtaacgctg catgacggtt
    15841 ggaacagcct gcagaggtat gtgagcgtac gcaaacgctc gacgcggctg gattttaaag
    15901 aggcaatgat gcaggcgcaa agcgatataa aatccgggaa gtgatgttaa attaaagggg
    15961 atgtgccgca tcccctgatt cctccgtctt gcatcaagac cggaatttcc gatattccga
    16021 ttttgccttt tcttccttgt ctgcgattgc ctttgcaagc tctcggacgc tgacgaaata
    16081 ttccgatttt cggctgtcgg acaatcggaa aatcggaaac ggaagctcgc accgtacccc
    16141 cgatgcttta aacgcgttaa agccgatatg ggcaaaaaag tctttgtgta cttccctcgg
    16201 cgggatatac gggcggccgc cgtatgcgat taaaagccct tgttctttac tgatgttcat
    16261 atatacctct ttctgttaat tctgctttgt ctgagtttcc gcttttttac ggcttggcgg
    16321 atgttcagcc tgcgtcggtg cataagtcct cctgttcggg cggttattca gccggccgcc
    16381 cctccgaaaa gtggcaggct gcacgggaag tagtgttttt ttccgtattt ctctgcctgt
    16441 atcaggttgc acaggcggtc gcaaaagccg tcaaattcgg catgcggcca gccttctatc
    16501 tcgttgacca ttttgctgaa cctcaattcc cgggcaatcc gctttgccgc tgcgtcacgg
    16561 cattcgtgtt ccaagaggac ggggtcttcc ttttccttcg gcggtgcagg ttcttgcacg
    16621 gtttgccaca agtccggttc gatttgccaa gtgtcggcgg caagcagggt gtctgttttg
    16681 cagtcgtaca gttcgcggat gcagccgccg tcggttttgt ctttgtcgat aaccagtaaa
    16741 agcacttcga tcggcgtgtc ttcaaacgcg ttttgtatgc ggttgagttc tgccagccgg
    16801 ttgccgatga tttcgcggaa tcgctgctcg cttttgcggt aggcgatgcc ggggaacagg
    16861 atgaagaatc cgaagcggcg ggcgttttcc aatcctttta aaacgaagat ttcatcggct
    16921 acgcccgatt tcttccacgg gtattcctgt gcgatacggc ttttttcgtc ttcgctcaaa
    16981 tcctttagct tgatggaaaa cggcggattc atgacggtgc agtcttgcgg ttcgccgtcg
    17041 gtataaagga aaaggcttgt gttgtatact ttggcggcag ggtagttttg caacaaggct
    17101 ttgcacgctt ccgcctgtat ctctacggcg cggaaatcgg acggttcgat gtactgctcc
    17161 agctgcccgc ttcctgccgc gccgtcaaat acgcccgggt gttcgccgca gtatcggcgg
    17221 actttggcgg caaccagccg cctcaagc
//
LOCUS       DAXFWM010000050        16998 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000050 DAXFWM010000000
VERSION     DAXFWM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16998
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.050"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(236..682)
                     /gene="smpB"
                     /locus_tag="ACFI8B_001519"
     CDS             complement(236..682)
                     /gene="smpB"
                     /locus_tag="ACFI8B_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC3834708.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(737..1747)
                     /gene="waaF"
                     /locus_tag="ACFI8B_001520"
     CDS             complement(737..1747)
                     /gene="waaF"
                     /locus_tag="ACFI8B_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC3834709.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISIETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(1878..1953)
                     /locus_tag="ACFI8B_001521"
     tRNA            complement(1878..1953)
                     /locus_tag="ACFI8B_001521"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1918..1920),aa:Asn,seq:gtt)
     gene            complement(2144..2896)
                     /locus_tag="ACFI8B_001522"
     CDS             complement(2144..2896)
                     /locus_tag="ACFI8B_001522"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC3834710.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFKGHLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            3246..3803
                     /locus_tag="ACFI8B_001523"
                     /pseudo
     CDS             3246..3803
                     /locus_tag="ACFI8B_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            complement(3781..4596)
                     /locus_tag="ACFI8B_001524"
     CDS             complement(3781..4596)
                     /locus_tag="ACFI8B_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3834711.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTFDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADVAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(5016..6161)
                     /gene="dapE"
                     /locus_tag="ACFI8B_001525"
     CDS             complement(5016..6161)
                     /gene="dapE"
                     /locus_tag="ACFI8B_001525"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC3834712.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGSLTVKGKQGHIAYPHLAVNPIHTFAPALLELTQ
                     EIWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            complement(6274..6894)
                     /locus_tag="ACFI8B_001526"
     CDS             complement(6274..6894)
                     /locus_tag="ACFI8B_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFC3834713.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(6928..7437)
                     /locus_tag="ACFI8B_001527"
     CDS             complement(6928..7437)
                     /locus_tag="ACFI8B_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC3834714.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            complement(7532..8083)
                     /gene="azu"
                     /locus_tag="ACFI8B_001528"
     CDS             complement(7532..8083)
                     /gene="azu"
                     /locus_tag="ACFI8B_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC3834715.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(8366..8788)
                     /locus_tag="ACFI8B_001529"
     CDS             complement(8366..8788)
                     /locus_tag="ACFI8B_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC3834716.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            8920..11670
                     /gene="secA"
                     /locus_tag="ACFI8B_001530"
     CDS             8920..11670
                     /gene="secA"
                     /locus_tag="ACFI8B_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC3834717.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            11815..13587
                     /gene="dnaG"
                     /locus_tag="ACFI8B_001531"
     CDS             11815..13587
                     /gene="dnaG"
                     /locus_tag="ACFI8B_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC3834718.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETIDRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            13774..15702
                     /gene="rpoD"
                     /locus_tag="ACFI8B_001532"
     CDS             13774..15702
                     /gene="rpoD"
                     /locus_tag="ACFI8B_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC3834719.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(15742..15927)
                     /locus_tag="ACFI8B_001533"
                     /pseudo
     CDS             complement(15742..15927)
                     /locus_tag="ACFI8B_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(16105..16425)
                     /locus_tag="ACFI8B_001534"
                     /pseudo
     CDS             complement(16105..16425)
                     /locus_tag="ACFI8B_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(16395..16742)
                     /locus_tag="ACFI8B_001535"
                     /pseudo
     CDS             complement(16395..16742)
                     /locus_tag="ACFI8B_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
     gene            complement(16802..>16998)
                     /locus_tag="ACFI8B_001536"
     CDS             complement(16802..>16998)
                     /locus_tag="ACFI8B_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834720.1"
                     /translation="EKISISQCPQCGGEMKYYNKPIEWENIRYSDGSKKRRVTRTTPA
                     LECKRNREHWYKVDPAEDKI"
BASE COUNT         4301 a         4863 c         4147 g         3687 t
ORIGIN      
        1 ttgtcaagcc ggtttgaaaa tgtgtataat tgccctcgtt atttacaaaa atttcaggaa
       61 aaatgaccgc actttaccct tggctaatgc caatttatca tcaaattgct caaacctttg
      121 acgaaggttt ggggcatcat gccgtgctga ttaaagcgga tgctggttta ggtgtagaac
      181 gtttacgcat caggcggcag ccttgcccgt accgtctgaa gcattgtttc cacaatcagc
      241 gcgtatgctt aatcaaccgc tgtttctcgc gtttccagtc ggcttccttc atactttgtc
      301 gtttatcgtg ctgtttctta ccttttgcca aaccgatttc catcttgatt tttccgcgtg
      361 aaaaatgcaa atccagcggc acgatggtgt aacctgcacg ttcggttttt ccgattaact
      421 tattgatttc cgactgtttt aataacagtt tgcgcggacg tacggcatcc ggtttgatgt
      481 gtgtcgaagc cgtaggcaaa gccgtaatat ggcagccgac cagataaaac gcatcttttt
      541 tccaatagat ataactctct ttaagctgta cgcgcgcggc gcggattgct ttgacttccc
      601 agccttccaa gaccaaaccg gcttcaatcc ggtcttcaat gaaaaaatcg tgaaatgctt
      661 ttttattgtt cgcaatagcc ataaacatcc tatcaatatc cgccgtcaga cggtataaat
      721 ccgaaaacag aagccatcat accgcctctt caaccgcctg cacaatcttc tcgggataca
      781 gcctgttgag gcagtcggta tgccccaacg ggcattcacg tttgaaacag ggcgaacatt
      841 ccaagtgcag gctgacgatt ttcgccctgt cgctcaaagg cggcgtatgc gtcgggctgg
      901 aagaaccgta aaccgccact accttcctgc ccaaagcagc cgccaaatgc atcagcccgc
      961 tgtcgttgca cacgaccgtg tccgccaacg acagcaaatc cattgcctgc gacaaatcgg
     1021 ttttgccgca caaattgaca cacatgccgt ctgaaaggca gttgatttct tcggcgattt
     1081 catcatcttt ttgcgaaccg aacaaccaaa cctgccaacc cgcctccgaa tagtgtttgc
     1141 ccaactcggc aaaatgcctt gccggccaac gctttgccgg cccgaattcc gcacccgggc
     1201 aaaaagccag aacaggcttt ccaagattca aaccgaaagt ttcaatagaa atttcccgcc
     1261 gccgttcatc aatggaaaac tcggggaatc ccgaatgccc gtcaaaatct tcctgactct
     1321 gatgcgcgag agccgtatat cggtccacca tcaaaggcag acgttcctta tccagcctgc
     1381 gtatatcgtt caacagaaaa taacggcttt cgccgacata acccgttctt tttccgatgc
     1441 ccgttgccag cgcgatgact gccgacttca aagaacccgg caacacgata accctgtcgt
     1501 atccgcgccg tcccaactcc ctgccgaccc gccaacggcg tttcagctcc aacgcaccgt
     1561 gtccgaacgg attttcaaga atctcattca cttccggcat acgctcgaac accgccatcg
     1621 accacttcgg tgcgaacaca tcaatcgtgc aaccgggatg aagtttcttc aaacggcgga
     1681 acaagggctg ggtcatcacg cagtcgccta tccaactggg agaaataatc aggattttga
     1741 tagacataaa acgaaaccga aatcagacag gcagaatttt accgcgaaac cgttggaaaa
     1801 cctatctcgc cgcattccga gcgccggacg tggaaatgtg aaaaagcccg aacattcaag
     1861 ttcgggcttc aaaattctgg ctccccgacc tgggctcgaa ccagggacct gcggattaac
     1921 agtccgtcgc tctaccgact gagctatcgg ggaatggggc gtattatagc atccggaaaa
     1981 aatgtgtcaa tccttaattt tggaaaaatg ggcgacaaaa cgacaagcat atgaatcaga
     2041 aagacattaa gaccgatgcc ttaaaaggat tgccgttgta tgaattttca cagccgtcat
     2101 cacaccatat ttaagcccga tgagccgttc tgcatttccc cgcttaaaac aatgccgtct
     2161 gaacttcgcc gcgttccaaa gccagtaaaa actgtttgcg gttcaacccg cccgcgtagc
     2221 cggtcagttt gccgtcgctg ccgatgacgc ggtggcaggg aatcatgaca gacaccttgt
     2281 tctgcccgtt ggcggcggca acggcgcgga cggctttggg gttgcccaaa cgctgcgcct
     2341 gctctttgta gctgcgcgtt tcgccgtaag gaatcgccaa gagcgcgtcc catgcctgct
     2401 tttgaaactc ggtgccaatc tgctccaaag gcgtggcaaa ggttttcaga tgacccttga
     2461 agtataagtc caattcctga cgcaaaaatt gcatccgccc gtcttcccga aacacaaacc
     2521 gtccgcgcaa ggctttttgg acggcggcaa tttcctgttc caaatacttc tgtccgacaa
     2581 actccagcag gcacaatccc ttaacgccga acaccgccaa catctcgccc aaaggcgtgg
     2641 caatagcggc acacaccagc tcgttcaagc tgtcgggata acgtaattcc aacagacgga
     2701 tggcgcggcg gatacggaca tattcttcag gcgcgcagcc gatattgtcc caaaaatccc
     2761 gctcgaactg cccggcttcg cattccgtca gattgggata cggcataacg ccgcactcaa
     2821 aaacccgggt ttcgaggcaa tggcggattt catcccattt tgacggcaga ttgtttaagg
     2881 aaggcagggt aatcattcgt ttgctccgta tccctatcat aaatttgacg gcgaaatccc
     2941 cgatttccgc cattcccgca cgccggtaca ggaacaggct gcggcggaaa ccttgagggt
     3001 ctggttgcgg caatgcctaa atatacaata tttccaaagc atcagggaaa ggaatgtttc
     3061 aacacacagg acgacacata aagcgtcgcc ctatgaaaaa tttcagacgg ccttcaaagg
     3121 gtcgtctgaa accacgattt ttgcatttgc gcattccggc acatcatcca accgtttcgg
     3181 cacattcctg ccgccgttga cagcctataa tgaatccact tattcatcaa gcaaaggaat
     3241 catctatgca aacccgcatc ctctccgccg tactgctggc tttttcaacc gctgcctttg
     3301 ccgggggcgc attcacgctg caattcgaca acccgtccga agacggcggc ttcacgcaaa
     3361 accagctttt gagcgcgcct tacggcttcg gctgttcggg cggcaatgct tcgcccgcgc
     3421 tgtcgtggta aaacccgccc gcagggacaa aaagtttcgt cctgaccgtt tacgataaag
     3481 acgcgccgat cggtctggac cggatgcact gggtggtcgc cgacattccc gccgatgtcc
     3541 gccgcctgcc cgcaggcatt accgcacaat gaggcaggcc gcccaaaggc gcgctgcaaa
     3601 cccgcacgga cttcggcgcg ccgggctacg gcggcgcgtg tcctcccgaa ggcaggaaac
     3661 accgctacga attcacgctt accgcgctga aagtcgccaa gctgccgaac atcaccgccg
     3721 aatccacacc cgtgctggtc ggtttctcca ctaaggcaaa cagcttgggc gaagcgaaat
     3781 tcacggtcga acaccgacgc tgacctgccc cgtgcgccga tatgccgccg gctccgcttc
     3841 cgagcggacc accgacggta cgcaaccgaa cagctctttg aagcgcgcgc tgaaacggga
     3901 cgggcagtca tagccaaccg cgccggcaat ctgcgctacc gaccattgcg taacctgcaa
     3961 cagcgtcagc gcgcgcatca tgcgtacatc cgtcagcagc gtacggaaat tcgtgtcctg
     4021 ccgcgccaac cgccgccgca acgccgcctc gctgcaatta agccgctgcg ccagcattgc
     4081 cgacgaccag tccgcagcca catcggcagt aatcagtttg cggatttgcc gcatcaggtt
     4141 gacgcctccg tacacggcaa aaccgatgcc gtcatgctgc aaccacgcca acacgccgca
     4201 aagcgcggcc tccgccgcat tgtgcggcac ttcttcatcc gccagtaccg cagccgcata
     4261 atcaaacgcc gcgcccatac gcccggggtg cggcagcttc accgcatcgc acactgcctg
     4321 cgccgtcccg tattgtgcgg cgaacctttc tacggtttcc tgttcgaaag caatccattg
     4381 cgcctgatag aggccgtctg aatcgggaat attgatgacg tcaaacgtct gcccgcctgc
     4441 caagccgaca gcctcacccg ccgcaatcct caattcgcgc cccgcccacc gcagcttctt
     4501 gcatccgcgc cgcacgataa ccacggtcgg ttcatatacc gccacgctgc ggtacaaggc
     4561 ggtatgatgt tgcacgatgc cgcctaaagc acccaatcgt tcacgcgtat gaaagtaatt
     4621 tgacggcacg gttatattcc tcaatgtttc aacacacagg gccgcacata aggcgcagcc
     4681 ctgtttaccc tgatttggaa ggggttactt cccaaaaaat aaaccaaaat tctactgccc
     4741 tgaagggcag ggtttcaacc atattccgtc ggtgtgcctg caacttagct ccctcccctg
     4801 tggggagggt cggagggagg gtaaaacgtg gcagatacag acaatatttc cgttaccgtc
     4861 ccaatgccct ctccctaacc ctctcccacg ggagagggaa tggattaccg ttcaaataaa
     4921 tcgctctaca taaaaatcaa tgcgttatct caaactcatg ttgggtctaa tcagattgtc
     4981 ggacatccat atccgacaac cagcccatct ttttttcaga cggccttttc agccaacaac
     5041 cgcgccaata tcctctcata caccgccgac agcttcggaa tatcgtccag ccgcacattt
     5101 tcgttgattt ggtggatggt ggcattggac gggcctaatt cgatgagttc tttcgcaatg
     5161 gctttaatga agcgtccgtc cgaagtgccg ccggtggtgg acaattcggc ctcaacgccg
     5221 caggtttcgg caatggcggt gcgtgccacg tcggtcagtt tgcccgcgtg ggtcaggaag
     5281 ggctgtcccg aacacgacca ctgcaaatcg tattgcacgc cgtgtttgtc caaaatggcg
     5341 tggacgcgtt gtttcagccc tgtttcggtg gactctgtgg agaagcggaa attgaatttg
     5401 acgttcaact cgcccggaat gacgttggtc gcgcctgtgc cgccgttgat attggaaatt
     5461 tgaaagctgg tcggcgggaa atattcgttg ccttcgtccc aaatttcctg cgtcagctct
     5521 aacaaggccg gggcaaaagt atgtatggga ttcaccgcca aatgcggata ggcgatatgc
     5581 ccttgcttgc ctttgacggt caggctgccc gacagcgagc cgcgccggcc gtttttaatc
     5641 atatcgccca atttgtccac ggcggtcggt tcgccgacga tgcagtagtc aatcaactct
     5701 ccgcgtgctt tcaacacatc gacgactttg gtcgtgccgt ccagtgcatc gccctcttcg
     5761 tcggaagtaa tcaagagtgc aatgctgcct tgatggtcgg gatgttcggc aacaaagcgt
     5821 tcgcaggcgg taacgaaaca ggcgatgctg gttttcatgt ccgccgcgcc gcgcccgtat
     5881 aatcttccgt cgcgctcggt cggttcgaac gggggcgaat cccatttttc aacaggacct
     5941 gtcggcacaa catcggtatg ccctgcaaaa cagacgacgg gagctttcgt gccgcgtcgc
     6001 aaccagatgt ttttggtgtc gccgaaatgg agttcttcag ccgcaaaacc gattttgtgc
     6061 aggcgttcgg caagcagttt ttggcaatct cggtcgtcgg gggtaacgga cgggcgggaa
     6121 atcaacgctt tggcgagttc tagggattgg gtttcggtca ttttgttcac tttggaaatc
     6181 aggtcgtctg aaatgtcctg aagctgattt tcagacgacc tccgggttgg attggtgtat
     6241 aagacgggca ttcgtacccg tcagttttct gaatcatttg ccgtgacagg cttcgtaaag
     6301 cggcagtaaa tcttccaccg tttcggctat ccattttgct acatcctgct tgcccaaatc
     6361 gtcgcgctca atgtgtttgc cgatgcagaa aaagtcttcg tcgttttgca accttcggtc
     6421 ggattcgctt tgttgtgcga cggtgcggta gtcgtcatat tcgctttccg cgccgtgcca
     6481 catatcgaat gcggcgtatt tttcggtatc gaaatcatcc agccagcggt tgtactcggg
     6541 cagcgcaatc ggcgacacgt cggctttgta gcaatgccag tccaagctga cgctcaggcg
     6601 gcggcggttg agcagtatcg acaggatggc ggcggaattt ttgtattggg cgtatttgaa
     6661 gtaggcgaag aaatgggcgc gaacctgcca gccgttacac cagcgttcga tatgcggcgg
     6721 ggcgaaaggt tcgctcaaat ctgcggcgac ctgctgtatc aactgctgcc atatctgcca
     6781 gttttccttg tagtcggctt tgatttgcgg aatgctctcg ggctggtatt ttttgagctg
     6841 ggcaaactgg aaaaacggga tattgaacaa atcgcagctt ttgggcgtca gcataatata
     6901 tccttgagac gattgtttca gacggcatta tttgcaccgg cgcgccgcca tgatttcgcc
     6961 gatttcggtc agcttctctt cggggataaa cgttttgccc atgtcgaaca aaggctcttc
     7021 aatcgccaga tgcacatcat atccctccac aaagcgtttg aacgcttccg catcggggat
     7081 ataagcgttg tctgcttcga gtttggcaaa ttcggcggaa acagccgccc agttgtcgta
     7141 cagcccgata tgttggcgca acagctcgtc cacgccttca cgggcttgcg gcgcgtattg
     7201 cagcagcagc gggaaaaagt tttcttcttc gtcctcatgg tgcagcggcg cggcaacgtt
     7261 gaaataccgg gagatttggc ggatggtttg caaaacaagc tgattgcagc cgttttcggc
     7321 gatatagccc gacaacatag caatctggcc gcaaaaacgg cgcactttgc cgtggcaggc
     7381 atacagcatt tcaatcggtt cggcaaaggt aacgctttgg gtttcaaacg gattcatgtt
     7441 ttcgttctca acggggactt ttcaagcagt cattttataa taaaacagcc tgcacaaagc
     7501 aggctgtctg tcttttgaaa ctttaagcgg attaatcgac caaagtcact ttgccgttca
     7561 tcaaagcacc gtgacccggg aaggtgcagg caaatttgta gtcgccgtca gccaatttgg
     7621 caggatccaa agtcagggaa gactcttcgc cgccgccgat cagtttggtg tgggcaacaa
     7681 cgcgcgcatc gtcaggtttg acatagtcgg tatcggcagc acctacgccg tctttaaata
     7741 cgccgtccat gtcttcagct ttggcaatca caaggttgtg acccatgctg gctttgggtt
     7801 gcgtaccggt atgtttcaga gtgatggtga actctttaca tgctttgctg acttggatgt
     7861 ctttggtgtt gaactgcata ttgtcgttgg attcgacagt tgccgcacag ttgccggcag
     7921 caggggcttc ggcagcatct gcaggagcag cttcggcggc aggcgcttcg gaagcgggtg
     7981 cttcaccggc aggagttgcc tcggcagcag gcgcggcagg ttcttgagag caggcagcca
     8041 aaccgataac ggcggcagaa atcagagcca gataagcttt catggtaaat ctccaatcga
     8101 taaaataata ttcggtttta cggaaatcaa agtgcaaccg ccattaacaa aaccttgaaa
     8161 aagattccgc cgtgttgcac aaacagatgt ttcggggcgg cattttgcta caaacttcat
     8221 ttgaaatcaa agcctgtttg caagtttaca atcgtttacc caaaaaaagg gcaatcttac
     8281 cccgaaccta tttctttaat attagggcag ccgtcccccg ccgcgcaaat attaacggat
     8341 atttacaaca tccctgccgg ccgccttatg ccccgtactt cctgaccagc tcctccagtg
     8401 cctccgccaa ttcgggatgc cgttcttcca actttgccgc cgccgaatcg aaactctcca
     8461 acgcagcctt actcaaatgc agggtattgg ttttcggcgg tttttccggt tttggaacca
     8521 gcctgaccga aacagaccgt atcgaagcat caagccctgc cagctgcggc aataccgacg
     8581 gggcaatcat cttcaaacgc gatgccgcca tattgtttgc cgccaaaagg acaagcctgc
     8641 cgtcttcgat acatgccgtc tgaaagtggg ggtgcaggtt ggcaggcagc agttttttca
     8701 cggcagcatc caaccgccgc cactgtcccg cctgtttcaa aagtccggaa agcagcgcgt
     8761 cccgcctgcc caactgttcc aaattcataa aacatacacc ccaaaagatt gaaataccgc
     8821 aaacgcgcct ttatttcaga cggcattagc actttgcaca aacgcttgtg ttaaaatcgc
     8881 gttttcgccc cactattata tcaggcgcag gaattattca tgctgacaaa cattgccaag
     8941 aaaatcttcg gcagccgcaa cgaccgcttg ctgaaacaat accgtaaatc cgttgccaga
     9001 atcaacgcgc tcgaagaaca gatgcaagcc ctaagcgatg ctgatctgca agccaaaact
     9061 gccgaattca aacaacgcct cgccgacggt cagactttgg acggcatttt gcccgaagcc
     9121 ttcgccgtct gccgcgaagc gtcccgccgc gtactcggta tgcgccactt cgacgtgcag
     9181 cttatcggcg gtatggtgct gcacgacggc aaaatcgccg aaatgcgtac cggcgaaggc
     9241 aaaaccctgg tcgccaccct cgccgtctat ctcaacgcgc tggccggcaa aggcgtgcac
     9301 gtcgttaccg tcaacgacta cctcgcctcg cgcgacgcag gcattatgga gccgctctac
     9361 aatttcctcg gtctgaccgt cggcgtgatt atttcagata tgcagccgtt cgaccgtcaa
     9421 aacgcctacg ctgccgacat tacctacggc acgaacaacg aattcggctt cgactacctg
     9481 cgcgacaata tggtaaccga ccaatacgac aaagtgcagc gcgaattgaa ttttgccgtt
     9541 gtcgatgaag tggactccat cttgattgac gaagcgcgca ccccgctgat tatctccggt
     9601 caggcagacg acaacatcca gctctaccaa atcatgaaca ccgttccgcc ccacctcgtc
     9661 cgtcaagaga cagaagaagg cgaaggcgac tattgggtcg acgaaaaggc acatcaggtc
     9721 atcctgagcg aaacaggtca cgaacacgcc gaacaaatcc tgacccaaat ggggctgctg
     9781 gcagaaaacg actcgctgta ctcggcggca aatatctccc tgatgcacca ccttatggca
     9841 gcattacgcg cgcactcgct gttccacaaa gaccaacatt acgttatcca agacggcgaa
     9901 atcgtcatcg ttgacgaatt tacaggccgt ctgatgtccg gccgccgctg gtccgaaggc
     9961 ctgcatcaag ccgtagaagc caaagaaggc gtggaaatca aacgcgaaaa ccaaaccctc
    10021 gcctccatca ccttccaaaa ctatttccgc ctgtacacca agctctccgg tatgaccggt
    10081 acggcagata ccgaggcttt cgagttccaa agcatctaca acctcgaaac cgtcatcatc
    10141 ccgaccaacc gccccgtaca gcgcaaagac ttcaacgacc agattttccg ttccgccgaa
    10201 gaaaaattcg aagccgtcgt caaagacatc gaggaatgcc acaaacgcgg gcagcccgtc
    10261 ctcgtcggta cgaccagcat cgaaaactcc gaactggtct cccgccttct gcaaaaagcc
    10321 ggactgccgc acaacgtcct caacgccaaa gaacacgaac gcgaagccct gattgtcgcc
    10381 caagccggca aagtcggcgc aattaccgtc gccaccaata tggcgggacg cggtacggac
    10441 atcgttttag gcggcaacct gaagcaccaa accgatgcca tccgcgccga cgaaaccttg
    10501 agcgacgaag agaaacaggc acaaatcgcc gcactcgaaa acggctggca ggcagaacac
    10561 gataaagtga tggaagcagg cggtttgcac atcatcggta cggaacgcca cgaaagccgc
    10621 cgtatcgaca accagttgcg cggacgttcc ggccgtcagg gcgaccccgg atccagccgt
    10681 ttctacctct cgtttgaaga cccgctgttg cgcctcttcg cactcgaccg cgccgccgcc
    10741 atcctcaacc gcctcgcccc cgaacgcggc gttgccatcg agcacaacct gctgacgcgc
    10801 caaatcgaag gggcgcaacg caaagtcgaa ggcagaaact tcgatatgcg caaacaggtt
    10861 ttggaatacg acgacgttgc caacgaacag cgcaaagtca tttacagcca gcgcaacgaa
    10921 attctgacca gcaaagacat cggcgacctg atgcaggaaa tccgttctga tgccgtcagc
    10981 gacctcgtgg atacctatat gccgcccgac agcatggaag aacaatggga catcccgaca
    11041 ctggaaaacc gcctggctgc cgaattcagg cttcaggaag acatccaatc ctggctgaag
    11101 gcggacaatg cgattgacgg tcaagacatc aaagagcgcc tgatcgaacg tatcgaaaac
    11161 gaatatgccg ccaaaaccga actggtcggc aagcaggcaa tggctgattt cgagcgcaac
    11221 gtgatgttgc aggccatcga caaccaatgg cgcgaacatc ttgccgccat ggactacctg
    11281 cgacaaggca tacacctgcg cagctatgcc cagaaaaatc cgaagcagga atataaacgc
    11341 gaagccttta ccatgttcca agacctgtgg aacggcatca aattccatat cgcctccctg
    11401 cttacttcgg ttcaaatcga acaaaaccct gtcgcggcgg ttgaagagca acccgtcggc
    11461 aacatccagt ccatccattc cgaatcgccc gatatagaag aacttttggg tcagtcgcaa
    11521 accgatctgg ttaccgaagc ctttaatccc gatgggacgg atttcagccc cgaagccttg
    11581 gaagcgcggg gacaaatcgt ccaccgcaac gacccctgcc cctgcggcag cggtttgaaa
    11641 tacaaacaat gccacggcaa attggcttaa gcgtttgaac gcaaatgccg tctgaacatc
    11701 cggtttccgg cttcagacgg cattttgcct gaaccgccac atccgaccgc cattccgaaa
    11761 aatcccgatt tcgtaccgtc cgtaccgaaa acagacatcc cgtcccccca catcatgatt
    11821 ccatccgact tcattgacga gcttctggcc aaaaccgata ttgtcggcat catcgacgag
    11881 caggttccgc tgaaaaaagg cggggcgaac tatatggcgt gttgcccgtt ccacaaggaa
    11941 aaaacaccgt cgttttcggt cagtccgacc aagcagtttt atcattgttt cagttgcggg
    12001 gcgcatggtt cggcgattgg ttttgtgatg gaacatcagg gactgtcgtt tccggaggcg
    12061 gttcagttcc ttgccgaccg cgtaggtatg attgtgccta aagtgcgcgg gcagaacgat
    12121 aatcccgaag tccgtgccga gcgcaagaaa aaacagcaga cgctggaaga aacgacggcg
    12181 gcggcagctg atttttacgc gcagcagctg aaattcaatc cggctgcgaa agcttatttg
    12241 gacaaacgcg gcttgagcgc ggaagtcatc gcgcattatg gtttgggcta cgcgcccgac
    12301 ggctggcagc ctttggcgca agtgttccaa ccgtatccga ataccgcgtt ggtggatacg
    12361 gggatggtga ttgacaatga ggggcggcat tacgaccgct tccgccatcg gattatgttc
    12421 cccatccgca atccgcgcgg gcaggtcatc ggtttcggcg gcagggtgct ggacgactcg
    12481 aaaccgaaat atttgaattc gcccgatacg cctttgttcg ataaggggaa aaacctttac
    12541 ggcttgtatg aggggcgtgc tgctgtaaaa gaggcagaac ggattttggt ggtcgaaggc
    12601 tatatggacg tggtcgcgct ggcacagttc ggcgtgggct acggtgtggc ggccttgggc
    12661 acggcgacga cggcggaaca cgtcaaaatc ctgatgcggc aggcggacag tatttatttc
    12721 tgtttcgacg gcgacagcgc ggggcgaaaa gcggcttggc gcgcgctgga aaacgcgctg
    12781 ccgcagttga aagacgacaa atcgctgcat tttttattcc tgccggaaga acacgacccc
    12841 gacagctaca tccgcgccta cggcaaagcg caatttgaag acgcgcttct gaatcaaagc
    12901 aagcctttgt cggaatattt ctgggaacac ctttcagacg gcattcatct caatacgcag
    12961 gaaggcaagg cggaattggt gaaaaccagt tcgccgcttt tggtgcagat taccgcaccg
    13021 gcattggctt atttgttaaa acaacggctt agcgagctgg tcggcatcga ccccgacaac
    13081 ctcgcgcaac tgctcggaca ggaagcgcca aagcggcacg tcaaacaaaa aaactacaaa
    13141 ctgcctccga tttccgtcaa acagcccgtt atgccgacat tggtgcaacg gcaaatccgc
    13201 agcctcttga taaatccgga ttgggctgca tatatagacc tgcccgatta tctggcgttg
    13261 gacggcgatt tcgcctgcct tgccaacctc gccgaaacca ttaaaaacca tccttccgtg
    13321 ccggcaaccg cacaggtttt ggaacatatg cgcggttcgc cctacgaaga aacaatcgac
    13381 cgcatcttcc ggtcggccct tcaatcggaa gaaatggaag gcggcggcga agaagattgc
    13441 gaaaacttcc aaatcggcat caaaaaactg ctcaatgagt taaaatacag ccaaattgaa
    13501 acattaaaac aaaaaagcct gcaatccggc ttaaatgaaa gcgagaaaaa acttttgctg
    13561 tcgctgctga ccgcaaaaca aaattgaccg gcggattccg ccatctgtaa accgttatgc
    13621 cgtctgaaaa gcattcaccc cggctgcaac aacgacacct gcagaacacc catcccaaaa
    13681 agccttcaga cggcatcaga gtaccctact ttgccacgcc ttcaggtgcg tccaaacgca
    13741 aaccgtcggc atcttgccaa cagaaagcag acaatgtcca aaaaccaaaa tcacgaagaa
    13801 taccaagacg atgcccgccc cctgacgatt gaggaacaac gtgcgcgcct gcgccaactc
    13861 atcattatgg gcaaagaacg cggctacatc acctactccg aaatcaacga cgcactgccc
    13921 gacgatatgt ccgatgccga tcaaatcgac aacatcgtca gtatgatttc cggtttgggt
    13981 atccaagtta ccgaacacgc ccctgatgcg gaagacatat tgttaagcga caatgccgcc
    14041 gttaccgacg atgatgccgt cgaagaagcc gaggccgccc tttccagcgc agattccgag
    14101 ttcggcagga cgaccgaccc cgtccgtatg tatatgcgcg aaatgggaca ggtcgacctg
    14161 ctgacccgcg aagacgaaat catcatcgcc aaaaaaatcg aaaacgccct gaaaaatatg
    14221 gttcaggcca tctccgcctg cccgggttcc attgcggaaa tcttagaact catcgaaaaa
    14281 atctgcaaag acgaaatccg cgtcgacgaa gtcgtagaag ccattatcga cccgaatgaa
    14341 gtattgctca acgaattggg cttggggcac ttggaaacca cagcacccga gaaaccttcc
    14401 aacgacaatt cggacgaaaa cgaagacgac gaagaatcgg aagaagatgc ggatgaagtc
    14461 tcggcagcca atctcgccga attgaaacaa aaagtcatcg gccactttgc ccaaatcgaa
    14521 aaagactaca aaaaaatgat cggctgtttg gaaaaacacc acagccggca caaagactat
    14581 ctcgcctacc gcgacgcgat tgccaacaaa ctgctggaag tccgtttcgc cacccggcaa
    14641 atcgacagcc tcagcagcag cctgcgcgga aaagtagaaa acatccgcaa actcgaacgc
    14701 gaaatccgcg acatctgcct cgaccgcgtc catatggaac gcgactactt catccaaaac
    14761 ttcctgcccg aaatcaccaa tctgcaatgg attgaagaag aaatcgccaa aggcagggtt
    14821 tggagcaacg cactcgaccg cttccgccac gccatcctcg aaaaacaaac cgagttggcg
    14881 gatatggaaa aagaaacccg catttccatc gaagagttga aagaaatcaa caaaaatatg
    14941 gtgtcgagcg aaaaagaaac cgcagccgcc aaacaggaaa tgattcaggc aaacttgcgc
    15001 ctcgtcattt ccattgccaa aaaatacacc aaccgtggct tgcagttcct tgatttgatt
    15061 caggaaggca acatcggcct gatgaaggcg gtcgacaagt tcgaataccg ccggggctac
    15121 aaattctcca cctacgcaac ctggtggatc cgccaggcaa ttacccgctc gattgccgat
    15181 caggcgcgta ccatccgtat tccggtacat atgattgaaa ccatcaacaa gatgaaccgc
    15241 atctcgcgcc aacaccttca agaaaccggc gaagaacccg attccgccaa acttgccgaa
    15301 ctgatgcaga tgcccgaaga caaaatccgc aaaatcatga aaatcgccaa agagccgatt
    15361 tcgatggaaa cccccatcgg cgacgacgac gattcgcact tgggcgactt catcgaagat
    15421 gccaacaatg ttgcgccggc cgatgcggca atgtacacca gcctgcacga agtaaccaaa
    15481 gaaatcctcg aaagcctgac accgcgtgag gcaaaagtcc tgcgtatgcg tttcggcatc
    15541 gatatgaaca ccgaccacac gctggaagaa gtcggcagac agtttgacgt aacgcgcgaa
    15601 cgcatccgtc aaatcgaggc aaaagcactc cgcaagctgc ggcatccgac aagaagcgac
    15661 cgtttgagaa gtttcttgga cagcgaagac agcaagctgt aaaccaaaaa accgcaggtt
    15721 tcaaatacct gcggtttttt cttacacaat aaacaacgct tccacatatc ccacactccc
    15781 atcccgtttc gccaacaact ccggctgaca gatttccacc agagagcagg ctttgcagcg
    15841 tttgccgtag tcgggcggcg gggtttgtcc gctgtttaag agttcgcgga cggcggctat
    15901 agttgcgagg gtttgggcgc gtaggccgag acctttgcaa aaatagtctg ttaacgaaat
    15961 ttgacgcata aaaatgcgcc aaaaaatttt caattgccta aaaccttcct aatattgagc
    16021 aaaaagtagg aaaaatcaga aaagttttgc attttgaaaa tgagattgag cataaaattt
    16081 tagtaaccta tgttattgca aaggtctcag gccgtctgaa aacacgacgg ggacacggtg
    16141 gcgggtttgc atataccaca gcgcgccctc agagacggtt tgccccgtca tttcttccaa
    16201 gcacaagcct tgggcgcaaa gctggatttc atcccccggg tcaggtttgg gcttgcctcg
    16261 tttgtattcc acaggtttca gacggcctgt ttttgtatcc acttccacca aatccaatat
    16321 gccgctgatg cccagcttat ccgccaaaac atgcactgtc cactcaaagc gcacgccctt
    16381 gcacgtttcc ggatcgcccg aatctttgcc gcaatgatat tgcacgcaat cggcatggga
    16441 ctttgctccg acacccgata ctgcgcccga cgcaacagca cattgccgtt ctgccgcccc
    16501 tgaagccgtc ccaaataaca cccgttttcg gtaaaaaacg ccaaattcac attattttcg
    16561 ccgcaaaacc ccaacagaaa cggcgacaca agcacattcc caaaacagaa aatatgcccg
    16621 atggaatgca ccggtaactg cgccaccttc ttacgctcct gctccaccac cagtgtctcc
    16681 cgctccttat gcagatagct gccttgggtg gtgatgtaga gtgtgttttg cagtttgcgc
    16741 atgggaaaac tccttaggtt ttgaccgtgc ttttggggtt aaacgagggg ctagctaaaa
    16801 attagatttt atcttctgcc ggatcaactt tataccaatg ctcacgattt cttttacact
    16861 ctaatgctgg tgtagttctt gttacccttc ttttcttgct accatcagaa taacgtatat
    16921 tctcccactc gattggctta ttataatatt tcatttctcc accgcactgt ggacattgag
    16981 aaatactaat tttctcta
//
LOCUS       DAXFWM010000051        16724 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000051 DAXFWM010000000
VERSION     DAXFWM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.051"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(309..1025)
                     /gene="trmB"
                     /locus_tag="ACFI8B_001537"
     CDS             complement(309..1025)
                     /gene="trmB"
                     /locus_tag="ACFI8B_001537"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC3834721.1"
                     /translation="MTDTPENQTPNDLPAGHSRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDNLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1167..2551)
                     /locus_tag="ACFI8B_001538"
                     /pseudo
     CDS             complement(1167..2551)
                     /locus_tag="ACFI8B_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(2668..4554)
                     /gene="uvrC"
                     /locus_tag="ACFI8B_001539"
     CDS             complement(2668..4554)
                     /gene="uvrC"
                     /locus_tag="ACFI8B_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC3834722.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KTLSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(4609..6786)
                     /locus_tag="ACFI8B_001540"
     CDS             complement(4609..6786)
                     /locus_tag="ACFI8B_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC3834723.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            6925..7875
                     /gene="trxB"
                     /locus_tag="ACFI8B_001541"
     CDS             6925..7875
                     /gene="trxB"
                     /locus_tag="ACFI8B_001541"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC3834724.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8031..8399
                     /gene="rpsF"
                     /locus_tag="ACFI8B_001542"
     CDS             8031..8399
                     /gene="rpsF"
                     /locus_tag="ACFI8B_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC3834725.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLAAAAEEAAAQ"
     gene            8400..8702
                     /gene="priB"
                     /locus_tag="ACFI8B_001543"
     CDS             8400..8702
                     /gene="priB"
                     /locus_tag="ACFI8B_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC3834726.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIQARILGRQAEEWQYRQGDCATVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            8709..8939
                     /gene="rpsR"
                     /locus_tag="ACFI8B_001544"
     CDS             8709..8939
                     /gene="rpsR"
                     /locus_tag="ACFI8B_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC3834727.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            8956..9408
                     /gene="rplI"
                     /locus_tag="ACFI8B_001545"
     CDS             8956..9408
                     /gene="rplI"
                     /locus_tag="ACFI8B_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC3834728.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(10031..10837)
                     /locus_tag="ACFI8B_001546"
     CDS             complement(10031..10837)
                     /locus_tag="ACFI8B_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC3834729.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(10838..11584)
                     /gene="pssA"
                     /locus_tag="ACFI8B_001547"
     CDS             complement(10838..11584)
                     /gene="pssA"
                     /locus_tag="ACFI8B_001547"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC3834730.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12166..12648
                     /locus_tag="ACFI8B_001548"
     CDS             12166..12648
                     /locus_tag="ACFI8B_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC3834731.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(12695..13906)
                     /locus_tag="ACFI8B_001549"
     CDS             complement(12695..13906)
                     /locus_tag="ACFI8B_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC3834732.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVDVGLCAVLAIV
                     LNSLLPDPKES"
     gene            14128..16566
                     /locus_tag="ACFI8B_001550"
     CDS             14128..16566
                     /locus_tag="ACFI8B_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC3834733.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDESEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         3833 a         4188 c         4713 g         3990 t
ORIGIN      
        1 atagaagttt agttgttttg ttaactatgc ctgaatgtac acctaagtct tcttttggta
       61 acgatgaata attagcaagc cgtagcctgc atctccgcca accgcgtgcc actatctgcg
      121 ggcggaataa actttaaaac cgatgtgcag gttaaaacct acaaggatgc gtgcggtata
      181 catgcgtttt tgccgttcaa accattgttt caaaacttgt attgaaacat cgcccgctgc
      241 tttatcttga aagaatgccg tctgtaagca aacatgcttc agacggcatt tttttatccg
      301 gcggtctgtt atccgatccg tttgaatacc aagtcccaaa cgccgtgtcc gaggcgtttg
      361 ccgcgcgctt cgaatttggt ttcggggcgg tagtccggag tgggggcgta gtctgccgcc
      421 gtattttgca ggttgtcgaa gctgctgagg acttcaagca tctgctgtgc atattcttcc
      481 cagtctgtcg ccaggtggat atagccgccg gttttgagtt tgggcagtag tttggcgatg
      541 aagggggctt gtatcagacg gcgtttgtgg tggcgttttt tgtgccacgg gtcggggaag
      601 aatatgtgga tgccgtcgag cgagccgtct tgcagcatat tttcgacaac ttctacggca
      661 tcgtgccgca tcacgcggat gttttccaaa tggttttcgt tgatgagttt gagcaggttg
      721 cctacgccgg ggccgtgtac gtcgatggcg agaaagtcgg tttcgggcag gcggcgggcg
      781 atttctgcgc ttgccattcc cataccgaag cctatttcga ggattttagg tcggctgctt
      841 ccgaaacggg catcaagatc ggccggtgtt gcttggtagt cgatgccgaa gctgtcccat
      901 aaggtatcga tggcgcgttg ctgtgcggcg gtcatgtggc tttggcggag gacgaaactg
      961 cggatgctgc gggagtgtcc ggccggcagg tcgtttggtg tttggttttc gggggtgtct
     1021 gtcataatgc tttcgtgcgc ttgtgcaatc aggatgccgt ctgaatgtga aatgactgtt
     1081 cggacggcgg gaaggaaagg ggcgattata cctgtttcgt ttttgcgggg taggtgtaac
     1141 ggtgttcaga cggcattttg ccactgttac agcagttctt gcaggacgtt tttttgcccg
     1201 tatccgttgt ttatggcttc ttgcaggcag tgacaggctt tttctttaat ccggtttgac
     1261 accgtgtccg ctgtaatgga tgcaggcgag gttgtagaac gccatactgt ttttttgcga
     1321 ggtggcttgt tcaaaccatt tgcgggcttt gttgtagtcg gcggctgtgc cctgtccgaa
     1381 gtaatacata ctgcccagat tggtttgggc tttgctgtgt ccgagttctg cggcttctgt
     1441 gtacagtttg agcgcggctg cgtattcggg tgcgcattgt aatccgtaat gcagggcttg
     1501 ggcttttgat aaagctgctc tgccctttgc cgcctaatgg cggaatcttt gatgccgtca
     1561 tattgctcgg gatggtttaa cgcgctgtcg gatatgagtt tctgataggc ggtaaggctg
     1621 ccggcttcgg ctgcctgccg gtaataatgc cgggcttttt ccgtatcgag ggctatgccc
     1681 aaaccgtagc ggcagatgtc gcccggtatc cgcaaacctt cgggatggcg ttcggcggcg
     1741 gctgcttcgg catgatgcag ggcaagtttg tggtttcttt ccagatactt gccttgcagg
     1801 tagatgtcgg ccagtgcggc atgggcatcg gggtcatttt cctgtgccgc ctgttggaac
     1861 cggtaaacgg cttcatcggg agccgtaggt aggatgagtt cggcaagcgg ggtgtaggcg
     1921 ggaatgtatc cttgttgcgc ggcggcgcgc aaatgattga cggcctgtgc cgtgtcggct
     1981 ttcgtccctt gtccgaaacg gtattgcagc ccgagttgcc aatgtgcttc cggcaagcct
     2041 tgtgccgccg ctttgcggta gagcaggtgc gccgtttcaa aatccggcgg cgtgccgtgc
     2101 cggcagtatt gggcgagcag gtattgcccg cggggattgt tgttttttgc gcttgttttt
     2161 gcccattcca gggcttcttc aaaatgtcgg gacgcgtaca gaaggtacat caggcggact
     2221 tgcgcttcgg gcaggcctgc ttctgccaga ggcctgtaaa gcggtatcag ggtttccggg
     2281 gcgcgccttc caattcttgg atatatgcca aattgtaacg cgcataggga tgcccctgtt
     2341 cggcggcgat gcggagcatt tccatacctt gttcgcggtc ttggtgcggg ctgtccgcgt
     2401 tcatcaggtg ttttgccaac ttgaatgcgg cggcgggatg atctgccgag gccgcttctg
     2461 tcagcagtcg gcggacttcg gtaaagttat aatattgttt ttgagacagg cgttccaagg
     2521 cctgccgata gggtaagtcg ggattgttca tatcagatcg ttttccgttt tgtcgaatca
     2581 tcgttattcg tcatgaagtt aggtttatat cggtatgccg gcctgctgta tcatattttt
     2641 acaggcggtt tttgctttgg cggcatacta atgcagatga ttataaatgg tttccgccaa
     2701 tgccttgctg atgccttcca ctttttccaa gtcctcgcgg ctggcggcaa tcacgccgcg
     2761 cagtccgccg aagcgggtaa gcagggcttg gcggcgtttg ctgcctacac cgggaatgtc
     2821 gccgagagac gaggtaacgc gtgctttgtc gcgtttttta cggtggcctg tgatggcaaa
     2881 gcggtgtgat tcatcgcgta cggtttgcaa taaatgcaag gccgggctat taggaggcag
     2941 gcggaagagt tcgccggtaa aaggcagtat gagttcttcc atgccggctt tgcgttcggg
     3001 gcctttggca ataccgacta aagggatgtg cagcccgagt tcttcccata cggatacggc
     3061 tacgccgatt tgccctttac cgccgtcaat caacacgaca tccggccatt tgacgctttc
     3121 gccgttggct tcggcttcct gtattttgcc ataacggcgc gtcaacactt cgcgcatggc
     3181 ggcgtagtcg tcgccgggtt tggcggtggt gatgttgtag cggcggtatt gcgagggttg
     3241 gatgttttgt tcgtcgtaca cgacgcagga ggcgatggtg gcttcgcctt gcgtgtggct
     3301 gatatcgaaa cattcaaggc ggttgattcc gtctgaattc atgccgagga ttttggcaag
     3361 ttcgtcgatg cggtgttgtt ggttgctttg ttgcaggtgg cgttgggtaa tcgccatttg
     3421 tgcgttttgt tccgccattt tcaaccaaac tttgcgttcg cctatggttt tggtaacgaa
     3481 ctgcatctgc ttgccgtgtt ctccttccaa agcctctttc aacgcatcgg gaacgggaaa
     3541 gttgctgatg atgatgtcgg gtttgctttt gcccagatag tgttgggcga cgaaggcttc
     3601 ggcgtaatct tgaccgtttg gttcggggtc gtttttggtg tcgggaaaga aacttttgtc
     3661 gccgacgtgc cgtccgccgc ggatgctgac ccagtgtacg cagacaaggc cgtctgaaac
     3721 ggcgagcgcg agcaggtcga tatcgttggg gttgttcggg tttttgctgt cgatgaactg
     3781 gttgctctgt ataatgccga gtgcttggat ttggtcgcga taacgggcgg cttcttcaaa
     3841 ttgcagattc gcggcggcgg tctgcatttt gtgttgcagg gtgcgggtca gttcgtcggt
     3901 tttgccgttg aggaaagtgg cggcttgacg cacgctgtcg cagtagtctt cttcgctgat
     3961 gtgtccgacg cagggcgcgg tgcagcgttt gatttggtac agtaggcaag ggcggtcgcg
     4021 gtgttcgaac acgctgtctt cgcaggtacg cagcatgaag actttttgca acacttgaat
     4081 gctgtcgcgc acggcgttgc tgttcggata cgggccgaag tattggttgg gatttttcag
     4141 cgtgccgcgg taatacgcca tttgcggata ttgatggccg ctgagcatca aataaggata
     4201 gcttttgtcg tcgcgaaaaa ggatattgta tttcggcgac aaggttttga tgaagttgtt
     4261 ttcgagaatc agggcttcgg cttcggaacg cgtgatggtg gtttcgatgt ggcgaacctg
     4321 tttcaccatc aacgcgatgc gcggcgaatg gtcgtttttt tggaaatagc cggacacgcg
     4381 ccgcttgagg ttgaccgctt tgccgacgta taagacgttg ccgccttcgt caaaaaaacg
     4441 gtacacgccc ggcagcttgg gcaggttttt gaggaagagc gggatgtcga attgttccgg
     4501 agaacggttt tctgtattca tatcaatcat ttgaaaaacg gcttcagacg gcatatcggc
     4561 agcggcagcc ttatccgttc ggaaaagctg tcgggcggtt atatgcggtc aatcgatttt
     4621 tacccatttc aggcgcaggg cattgcccaa taccgaaacc gagcttgccg ccattgccgc
     4681 gcctgctatg acgggattta aaaagccgag cgcggcgagc ggaatgccca atatattgta
     4741 gaagaaggcg aaaaataggt tttgcttgat gttttccaac gttgcctgcg atatcagcag
     4801 ggcatcggcg agctgattga ccgaatgctg catcagcgtg gcggaggcgg tgtgttcggc
     4861 aacgtccgca ccgcctttca tggcgaagct gacgttggcg gcggcaagcg cgggcgcgtc
     4921 gttgatgccg tcgccgacca tcgccacggt tttgccggcg gctttgagtt tctgcacttc
     4981 ggcggctttg tcgcacggac tcatattacc gaaggcgtgt gcgatgccca gttgtttggc
     5041 gacgtattcg accgtacttt ggttatcgcc gctcataata tagacatcga tattgtgttt
     5101 tttcagacgg cctatggctt cggcggtatc ggctttcaac gcgtcggaga gtgcgaatgc
     5161 gccgatcggt ttgccgttta cagatacggt aaccgcactg gcgatttccc aaacgccgtc
     5221 tgaaaacttc ggcaaggtca gttcggcaaa ttcggctttg cctgatttca ccaaacccac
     5281 gccttccact tcggcggtaa tgcctgctcc gacaacggtt tgcgcatttt gtgcagcggg
     5341 aatctccaaa ccgcgcgctt gtgcggcgga gacgatggcg cgggcgagcg ggtgggcggc
     5401 gttttgctcg acggcggcgg cgatgcggta caaagcgtct tcgtcaaagc cgctgtcggg
     5461 aacgtaataa acggcggcaa cctgcggcct gccttcggtc agcgtaccgg ttttgtccaa
     5521 tacgacggca tcgacgtggg ctgcttcctc cattgccgcc gcgtctttaa accaaatgcc
     5581 gtgtttcacc gctttgccca tgccgaccat aatcgcggca ggggtcgcca gaccgagcgc
     5641 gcacgggcag gcaatcacca aaacggcaac ggcgtgcatc agtgcgaccg tccaatcgcc
     5701 cttaatcagc caagtaacga taaaagtcag aagcgcgatg cccacgacag ttggcacaaa
     5761 taccgccgcc gctttatcgg ccacgcgcgc aatcggtgct ttactgcctt gtgcttcaga
     5821 gagcgcgttc atcatgtcgc cgagcagggt ttggctgccg agctgcgcgg cgcggtacac
     5881 cacgctgcct tcggtcatca gcgcgcccgc caacactttg ccgcccgcct ttttctcttc
     5941 gggattggat tcgccggtaa ggtggctttc gtccgcccaa ccgctgccgc tttcgataat
     6001 gccgtcggca gcgatgcgtt cgccgtggtt ggtgcggata aggtcgccga tttgcacttg
     6061 gtcgatgggc agttgtttcc attcgccgtt gcgttgcacg ttgacttggg tcggcgtgag
     6121 ttttagcagt aagcccaagc tgttcaggct ggattttttg gtgcggtgtt ccaaaaactt
     6181 acccagcgac acaaaaccga tcaccatcac gcccgcttca aaatacacat gcgccatacc
     6241 gtgcgccgca tgcgaactga aaaacagcat ataaacggaa tacaggtaaa tcgacaccgt
     6301 gccgatggta acgagtacgt ccatattcgc cagcccgcct ttaatgcttg cccacgcgct
     6361 tttgtaaaac gggattgcca gccaaagttg cactatgctt gccagtacaa actgccatac
     6421 aggcggaatc atccaatcgt gccgtgtcca attcagccct tttagcatca tccctaccat
     6481 accgataagg aacgggatat tgatggtcag caaaagccac aaccgccagc cgatatggtg
     6541 ttctgcttca ggttgcggca atgtatcttc cgttttttcc ttcgcgccgt aaccggtttt
     6601 ctcaatgatt ttggcaatgt cggcaaccga ggttttgctg ccgtcaaacg taacctgcgc
     6661 ttcctcactg gcaaagttca ctcccgccga ttcgacaaaa tcttttttgt tcaacacttt
     6721 ttcaatgcgc gaagcacatg cctgacaggt catcccctcg atttggaaac ggattttttg
     6781 ttgcataagt ttgattcccg ttttgatgtt cagacggcag tcaaggctgc gggatgccgt
     6841 ctgaacgccg gtatggtcaa aatttctcaa acgcgccgat aattcgggta gaatccgaaa
     6901 acttgacaca gcaaaggaaa aacaatgtcc caacaccgca aactgattat tttgggttcc
     6961 ggccccgccg gatacaccgc cgccgtctat gccgcacgtg ccaatttaaa ccccgtcatt
     7021 attacaggta tcgcgcaagg cgggcaactg atgacaacga ctgaagtgga caactggcct
     7081 gccgatgccg acggcgtgca agggccggaa ttgatggcgc ggtttctcgc ccacgccgaa
     7141 cgtttcggga cagaaatcat ttttgaccaa atcaacgccg tcgatctgca aaaacgcccg
     7201 ttcgcactca aaggcgatat gggcgagtac acttgcgatg ccctgattgt cgccaccggc
     7261 gcgtccgcca aataccttgg tttgccgagt gaggaagcgt ttgcagggaa aggcgtttcc
     7321 gcctgcgcca cctgcgacgg tttcttctac aaaaaccaag atgttgccgt agtcggcggc
     7381 ggcaatacgg cagttgagga ggcactctac cttgccaata tcgccaaaac cgttacgctg
     7441 attcaccgcc gcagcgagtt ccgtgctgaa aaaatcatga ttgacaaact gatgaaacgc
     7501 gtggaagagg gcaaaatcat cctcaagctg gaaagcaacc tgcaagaagt actgggcgac
     7561 gatcggggcg taaatggcgc attattaaaa aacaacgacg gttctgatca acaaattgcc
     7621 gtcagcggca tctttatcgc catcgggcac aagccgaata ccgatatttt caaagggcag
     7681 ttggaaatgg acgaagccgg ctacctgaaa acaaaaggcg gcacggcgga caatgtcggt
     7741 gcaaccaata tcgaaggcgt atgggcggcg ggcgacgtaa aagaccatac ctaccgtcag
     7801 gcaattacca gcgcggcctc cggctgtcag gcggctttgg acgcggaacg ttggctgggc
     7861 agccaaaaca tttgaatgcc gtctgaaacc gaaattttcg gcggaatgca agtttttcgt
     7921 accaaatcct tgattccgtc ggcaaacccc aatataatgc ctgctctttg aaccttgcct
     7981 tctgctttcg catggcagaa ggctgttttt accatccata aggagataac atgcgtcatt
     8041 acgagatcgt gtttatcgtt catcctgatc aaagcgagca agtgcccgct atggttgaac
     8101 gttacaaaac catgattacc gaggcaaacg gtaagattca ccgtttggaa gattggggcc
     8161 gccgccaact ggcttacccg attaacaaaa tccataaagc acattatgtt ttgatgaaca
     8221 tcgaaaccac tcccgaagtt gttgaggagt tggaaaccgc gttccgtttc aatgatgcgg
     8281 tattgcgtca tctgaccatc aaaaccaaac acgccgttac cgaagcatcc cctatgttgg
     8341 gcggcgaaaa ggctaagaac ctgttggccg ccgcggctga agaagcggct gcccaataat
     8401 tgggattcac taatcttgtt tcgcttgccg cattgattga aaaggctttc cctattcgat
     8461 atacgcctgc cggaatccct gttttagata ttattttaaa gcacgaatcg tggcaggagg
     8521 aaaatgggca gcaatgcctt gtccaattgg aaattcaggc acgaatttta ggcaggcagg
     8581 cggaagagtg gcagtatcgg caaggcgact gcgcaacagt cgaaggtttt ttagctcaaa
     8641 aaagcagacg ttccctgatg ccgatgctca ggatacaaaa tattaaagaa tataaaggtt
     8701 aaacgacaat ggctcgtcaa tcattcaaac gtagaaaatt ctgccgtttc acggctgaaa
     8761 aaatccaaga agtcgattac aaacaagttg atttgctgaa agactttatc tctgaaaacg
     8821 gtaaaatcat tcctgcccgc atcacaggaa cgaaggcatt ctaccaacgc caattggctg
     8881 ttgccgtaaa acgcgcgcgc ttcttggcac tcctgcctta taccgaccaa cacaaataat
     8941 tttggagttt aaatcatgca aattattctg ttagaaaaaa tcggcggtct gggcaacttg
     9001 ggcgacatcg ttaccgtaaa aaacggttat gcccgcaact tcttgattcc tgctggtaag
     9061 gcaaaacgtg ctactgaagc aaacatgaaa gagtttgaag cacgccgcgc cgaattggaa
     9121 gccaaacagg ccgaaatttt ggcagatgcc cgagcccgtc aggaaaaact ggacggtcaa
     9181 accgttaccg ttgcacagaa agccggtgtg gacggccgcc tgttcggttc cgttaccaat
     9241 gccgacattg ctgccgcaat cgttgctgcc ggcatcgaag ccgtgaaagc aaatgtacgt
     9301 ctgccgaacg gtcctctgaa agccgttgga gagtacgaag tggaagtggc tttacacact
     9361 gatgccgttg ctaaaattac tgttgccgtc attgccgcag ccgagtaatt gaaattatca
     9421 gatgccgtct gaaaccctgc cgattatcag aatgtggttt cagacggcat ttgttttgtt
     9481 ttccgtatat tctgcaaaaa tcgggacggt tgttttttat ttgcctgagg tctttgcaga
     9541 aatggtttgt taacgaaatt tgatgcataa aatgcgccaa aaaatattgg ctgtgtttta
     9601 atatagtgtt gataagcgta cctggtttca atatattcaa taggataggt ctcttcccaa
     9661 ccgaaaaaat aattgtcttt tctcttgtaa atagttgaaa tagattgtca aaatacatcc
     9721 taaaaaattt aataacttat atgattgcaa aggttgcggc ttgtatgccg gtcattatgg
     9781 cggaaatacc gtttgggagg cagtcgcatc gcatggagtc ggcacggcag gcgatgcgcc
     9841 gatgccttcg ggcgtgaagg cagggaaatc ggcagtttgg aaaaaggcaa tggaatccgc
     9901 aggtaggtgt cgattgggtg gtgaaaggca ataagtcggg cgcacgctca gactgcctat
     9961 ccccttgtcg gaacgggagg cagggcagct ttgtcctgac taattgcttg tgcaaacggt
    10021 aagaaggttt ttaaagcagg ttgtacagca tttttccggc aatcaaaagc aacataatgc
    10081 cgaaggattc tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca ccgagcgggg
    10141 caaaggcaat ggttgccgcg ctgaggacgg cgacggcggg caggtaaagg aagcccagcg
    10201 acccttcggg caatcctgca atattcagac cgttgaccag atacgatatt gcgccggaga
    10261 gtgcaatcgg ccaggcaagg ccggatgatg tgccgatggc tttatgggcg gggaagccgc
    10321 agtggattaa gaaggggacg gaaagtgaac cgccgcctat gccgacccag ctcgacattg
    10381 cgccgaacag tgtggaaacc gcagtcagtc cgggcagccc gggcagcggg cgggatgccg
    10441 tctgacgacc ggtatgcagt gttttgaatg cgacggcggt taaaaacagg atgaagaaaa
    10501 tttgaagccc gaacgcgggg atatattttg cggagagtgc gccagcgaat acgccgaata
    10561 tcatacccgg catcatcgca aatatggttt tccagtcgac cgcctgtttt ttgtgctgcc
    10621 ccaacatact ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg acggcgaggt
    10681 gttgcgcgta aggatgttgt gccaaaccct gcaaatcaag cacccataaa acgacaggga
    10741 caatcagcgt accgccgcct acaccgaaca ggccggcaat aaaacctgcc gcactgccta
    10801 cggcaagcag gattaagata atgtcccaat gccacattta atccgccttt ctatattttt
    10861 tccaaaatcg gcgtgccgcc ataatgtagc cggacaggct gtatccgaga aagaacagga
    10921 aaaggacgag cgacggttcc caagtgacca gaagcagcag taagacggca agcagcattc
    10981 cgacaaaggg gacttgtctg cggatgttga tttctttaaa actccaaaaa gggatttgga
    11041 caatcatcga caggccggca aacagtgtga tgcctaatgc ccaccagtgg acggcgggga
    11101 atttttcgac gctgtggttg acccaaatca gcccgacaat cagcgcggcg gcagtcggac
    11161 tgggtacgcc gataaaccag cgtttgtcca ccttgccgat gagcgtgttg aacagcgcga
    11221 ggcgtagggc ggcgcaggcg cagtagatga aggcgacgga ataaccgatt ttgccgaact
    11281 gccaaagctg ccatttgtag gcaataagcg cgggggcaac gccgaagctg accatgtcgg
    11341 caaggctgtc gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc gccacgcgcc
    11401 cgtccatacc gtccagcaac atagagatga ataccgcgat ggcggcggtt tcataacgtc
    11461 cgtgcatgga ttgggtgatt gcgtaaaatg cggaaaacag cgcggcgatg gtaaaggagt
    11521 tgggcagcag gtagatactg ttttgacgca gggagcgttg cggggtgttg gtcggggttt
    11581 ccatagctga agtgtctcac ggacgtggag aatcgatagg ggattataac ccaaaccatt
    11641 atcgaacaat acgcccttta ggatgaagcc ctcggaggcg gtttgttttt ctgatggcgg
    11701 atgctttgcg gggcggtttg actctgcctg ccaccgggtg tgttgtttcg gattttgccg
    11761 gatggatatt gttgccgggc atttctgcgc ttgccggaag gtatcggagg gagggcatcg
    11821 gaatatgcgg cgcgccgttc aaaccggggc ggtatttccc tattttcagg gagtttgtag
    11881 gtttcaacaa attgaaagat attttttgca tttccgaccg tatgctgatt ttgaagcggg
    11941 ataaattata ggacaaggtt tttcgcccgc cgcaaaggtt ggctgcaatg ccgtttgaaa
    12001 gcaacggctt gccgctaagg cggcaatatg aggatttgcg ttgccgaaac cggtttgcca
    12061 tatcaggcgg tgcaatctgc ggggagggat aagccgataa ggcggactta tcctgttttt
    12121 tgtttataat gcaacagaat ttggaggaac cgtcgggaat ttatcatgat gaaaaagata
    12181 ctggccgtat cggcactatg cctgatgact gcggcggcac gggctgccga tacttacggc
    12241 tatctcgccg tttggcagaa tccgcaggat gcaaacgatg ttttgcaggt taaaaccaca
    12301 aaagaagatt cggcgaaaag cgaagcgttt gccgagttgg aagccttttg caaaggtcag
    12361 gacacgcttg cgggcattgc cgaagacgag ccgaccggat gccggtcggt cgtgtcgctg
    12421 aacaatacct gtgtctcgct ggcatacccg aaagccttgg gcgcgatgcg cgttgaaaac
    12481 gccgtcgtga ttacttctcc gcgttttacg agcgttcatc aggtcgcact caaccagtgc
    12541 ataaaaaaat acggcgcaca gggacaatgc ggcttggaaa cagtgtattg cacgtcatct
    12601 tcttattacg gcggggctgt tcgctcctta atccaacacc tgaaataaaa aatgccgtct
    12661 gaaggatgtt cggacggcat ttccatatcg acggtcagga ttctttcgga tcgggcagca
    12721 ggctgttcaa cacaatggcg agtacggcgc acaagcccac gtcgacaaag ctgaagctgc
    12781 ccaatttgag tgtcataccg ccgatgcccg tggtcagtac cgagctgacg atgaccaggt
    12841 ttttcggcag catcaaatcg actttggcat caatcagggt tttgatgcct aaagaagcaa
    12901 tcgtgccgaa cagcagcagc ataatgccgc ccatcacggg catcggaatg gaagccaaaa
    12961 acgcattgaa tttgccgaaa aacgccatgc agacggcaaa aaccgccgcc caagtcatga
    13021 tgacggggtt gctgtttttg gtaatcatca ccgcacccgt tacttcgccg taggttgtaa
    13081 ccggcgggcc gccgatcaga cctgcaacgc acacgcccaa gccgtcgccc gcaagggttt
    13141 tgtccaagcc cgggtctttt gtgtagtctt ttcccgttac attgccgatt gccatgatgc
    13201 cgccgatgtg ttcgatggcg ggggcgacgg caacgggcag cataaacagt gccgcctgcc
    13261 agttgacctg aggcgtttca aaatggggaa cggcgaacca gggcgcgtgt gcaatgcttg
    13321 tcgtgtccac cagtcccatc agcagtgcca aaacataacc cgaagcgaca ccgatcaaga
    13381 tgggaatcag cttcatcatc ctgctgccga aaaccgatac gatggcggta acggcaaagg
    13441 taaagccgga aagaatcagc gaatcggtat agtcgatgac ctgtttgccg tcagcctgac
    13501 ccattgccat gctgcttgcc gccacggcaa cagacagacc gatgaccatg atgacagggc
    13561 cggtgacgac cggcggcagc agtttgtgta ccgccgccag tccgcgccaa cggatcagcg
    13621 cggcaaacac aaaatacata aagccggcgg caaacagccc gaacatggtg gaaggcagcc
    13681 cccattcgcc gacggagtag ataatcggcg cgataaaggc aaacgaagaa ccgagaaaaa
    13741 tcggtacttt gcgtttggtt gtgatttgga acagcagcgt tcccaagcct gcgcccaaaa
    13801 gcgcaagagc cggattcaga ccggtcagca ggggaaccag caccattgcg ccgaatgcca
    13861 caaataaaat ctgcgcgccg gaaacggcaa gtttcagttg gttcatacca tcttctttca
    13921 atcggttcaa acggcacgga ttatactggt ttgtcgaggg gctgcgtaaa aatacatacg
    13981 tcgggcaaag atatgccgtc tgaaaggcaa cgtctgtctg ttgcaggatg aaagcaggag
    14041 aaatggagta taatccgaca ctttccatat tcaatccgca agccgtcccg aacccttatt
    14101 tcagacggca ttttccggta atacggcatg acagaaaaat cccataaaaa aacagcaaaa
    14161 ggcagggcgg gaagcccgtc cccaacgtcc gcacgcaaca agaaagccga caacggcgcg
    14221 cggggcaata aagtttccga gcggctcaag gcggtcaaag agctgcagaa aaccgaaacc
    14281 aaaaaggcgc gtcccgaaca tgtcgtcaac ctgattgccg acgcgctgtg gctgatgggt
    14341 ttggcggcaa ccctgtattt ggcgatttcc ctgatcagtt tcgatatggg cgatccgtct
    14401 tggtcgcaca gttcaccggt tgtggaagat gccgccaatt ggggcggact gttcggcgcg
    14461 tatgttgccg atgtcggcta ttatcttttc ggctggtcgt tctggtggtg gatagcggct
    14521 gcctgcgtcg tgctgtataa aaatttccgt ctgcacgcaa aacagacgga aaacgaggca
    14581 tacaaccaca aaatcgctgc cgccgcgctg tttgtcctga cggtcttcag ccccgtcttg
    14641 gagtattttg tgctgggcgg aaaatatgcc gactccctgc ctgtcggagc aggcggtatg
    14701 gtcggcatac gtgtcggcgc agtgtttgcg tggctgttgg ggaaatcggg cagcctgctg
    14761 attatcctgg ttgttctgct gttgtcgttg tccctgctgg tgcagatttc atggctggaa
    14821 tttttgaacg gtgcgggcag ggcggttcaa aaccgcctga gtgccttatc cggcaaggtc
    14881 atggctttga gaaaacgccg gccgaatacc aaaacagacg gtgtcgatac ccaaaataca
    14941 cggcgcatgg tgaaagaagc caaaaacatc actgccaaac ccgttgcctt gcccgaaggc
    15001 agcagcagca accgcaaatc cgtcgcggtt tccgttgcgc cgccgcctaa aatccaacct
    15061 tcgctgtttg aagacaatga agtacagcag aacggcgaat accacaagcc tacattgaac
    15121 ctattgcgga ttcccgacag cgagccggtc agcatcaatc ccgccgaatt ggagcgcact
    15181 gccgaactaa tcgaatccaa actggcagaa ttcggcatcg gcgtacaagt cgtatccgcg
    15241 acatccggcc ccgtcatcac gcgctacgaa atcgaacccg cgcaaggtgt caaaggcagc
    15301 caaattgttg ccctgtcgaa agatttggca cgctctatgt cgctgcaatc cgtgcgtatc
    15361 gtcgaaacca ttgcgggcaa aaacacgatg ggcatcgagt tgcccaacga caaacgccaa
    15421 gacgtgatgt tgagcgaaat cttgtcctcg cccgtgtttg ccgaagccaa atccaagctg
    15481 accgtcgcgc tgggcaaaga catcgccggt acgcccgtcg tcggcgactt ggcgaaaatg
    15541 ccgcaccttt tggtcgccgg catgaccggt tcgggcaaat ccgtcggcgt gaacggcatg
    15601 attatgtcta tgcttttcaa agctacgccc gaagaagtcc gcttcattat gatagacccg
    15661 aaaatgctcg agttgagcat ttacgacggc attccgcacc tactctgtcc cgtcgttacc
    15721 gatatgcgcg aagcagggca ggcgttgaac tggtgcgtcg ccgaaatgga aaaacgctac
    15781 cgcctgctct cccacgccgg tgtacgcaat ctggaaggct tcaaccaaaa agtcgaacaa
    15841 gccaaagcgg caggcaagcc gctgctcaat ccgttcagcc tgaacctcga cgaatctgag
    15901 ccactggaaa aactgccgat gatcgtggtc gttatcgacg aacttgccga cctgatgatg
    15961 accgaacgca aagctgtcga gcagcaaatc gcccgcctcg cccaaaaagc gcgcgccgcc
    16021 ggcatacaca tgattgtcgc cacccaacgc ccaagcgtcg atgtcgtaac cggtctgatt
    16081 aaagccaaca tcccgacgcg tatggcgttt accgtgcaaa gcaaaatcga cagccgtacc
    16141 atcctcgacc aaatgggcgc ggacgaactg ctcaaatatg gcgattcgct gttcctgcaa
    16201 ccgggcagtg ccgaaccgac ccgcctgcaa ggcacgtttg tttcagacgg cgaagtgcat
    16261 caagtcgtca actacgtcaa atcgcaagcc ccagccgact atattgaagg tctgctcagc
    16321 ggcgaagccg cgctggaaac tgccaatatc gttaatccga atgcagacag cgacgaatta
    16381 ttcgatcagg cagtcgccta tgttttggaa agcaaaaaaa cctccatttc gtctttgcag
    16441 cggcagttgc gtatcggcta taaccgcgcg gcaaacctga tggaggcact ggaaaatgcg
    16501 ggtatcgttt ctccctccga cctcaacggc agccgtaaaa ttttggcgca caaggaccat
    16561 ttatagcccg tatcgcaaaa tgccgtctga acggcggaat tggcgtttca gacggcatga
    16621 ggcctttgca aaaaagccct tccttcgaca tccgaaaccc aaacacaggt tttcggctgt
    16681 tttcgcccca aatacctcct aattttaccc gaatatcccc ttaa
//
LOCUS       DAXFWM010000052        16568 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000052 DAXFWM010000000
VERSION     DAXFWM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16568
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.052"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(59..988)
                     /locus_tag="ACFI8B_001551"
     CDS             complement(59..988)
                     /locus_tag="ACFI8B_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC3834734.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1117..1869
                     /locus_tag="ACFI8B_001552"
     CDS             1117..1869
                     /locus_tag="ACFI8B_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC3834735.1"
                     /translation="MVLRRDFLAWCDETLQTASFFKDYAPNGLQVEGREYIGKIVTSV
                     TASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPL
                     DVHSTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVA
                     IGNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGH
                     HATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            1992..2573
                     /gene="petA"
                     /locus_tag="ACFI8B_001553"
     CDS             1992..2573
                     /gene="petA"
                     /locus_tag="ACFI8B_001553"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC3834736.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2592..3941
                     /locus_tag="ACFI8B_001554"
     CDS             2592..3941
                     /locus_tag="ACFI8B_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC3834737.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            3956..4744
                     /locus_tag="ACFI8B_001555"
     CDS             3956..4744
                     /locus_tag="ACFI8B_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC3834738.1"
                     /translation="MKQAMKNWFAALLSAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5076..6866
                     /gene="rmuC"
                     /locus_tag="ACFI8B_001556"
     CDS             5076..6866
                     /gene="rmuC"
                     /locus_tag="ACFI8B_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC3834739.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(6933..7565)
                     /locus_tag="ACFI8B_001557"
     CDS             complement(6933..7565)
                     /locus_tag="ACFI8B_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC3834740.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7658..8482
                     /locus_tag="ACFI8B_001558"
     CDS             7658..8482
                     /locus_tag="ACFI8B_001558"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC3834741.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8551..9114
                     /locus_tag="ACFI8B_001559"
     CDS             8551..9114
                     /locus_tag="ACFI8B_001559"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC3834742.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            9198..9635
                     /locus_tag="ACFI8B_001560"
     CDS             9198..9635
                     /locus_tag="ACFI8B_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC3834743.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            9856..10125
                     /locus_tag="ACFI8B_001561"
     CDS             9856..10125
                     /locus_tag="ACFI8B_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC3834744.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            10125..11900
                     /gene="ptsP"
                     /locus_tag="ACFI8B_001562"
     CDS             10125..11900
                     /gene="ptsP"
                     /locus_tag="ACFI8B_001562"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC3834745.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(12027..12968)
                     /locus_tag="ACFI8B_001563"
     CDS             complement(12027..12968)
                     /locus_tag="ACFI8B_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3834746.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(12977..13852)
                     /locus_tag="ACFI8B_001564"
     CDS             complement(12977..13852)
                     /locus_tag="ACFI8B_001564"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC3834747.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(14035..14111)
                     /locus_tag="ACFI8B_001565"
     tRNA            complement(14035..14111)
                     /locus_tag="ACFI8B_001565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14075..14077),aa:Met,seq:cat)
     regulatory      14260..14359
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14450..16351
                     /gene="thiC"
                     /locus_tag="ACFI8B_001566"
     CDS             14450..16351
                     /gene="thiC"
                     /locus_tag="ACFI8B_001566"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC3834748.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYQNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         3975 a         4340 c         4635 g         3618 t
ORIGIN      
        1 gttttcttaa atataaaaaa atagaaaaat agcccgggta tagtttgaac aagggaaatc
       61 agaccggttc caactcactt aatccgggca agtccgcgaa gccgcgttca cgtatgattt
      121 ggcaaaagtt gttcagataa cttttatctg tgtcttcagt gcggatggcg gcatacagtt
      181 tgctttgcag tccgtcggca gtaatttggc ggtggacgac atagcctttt tcaaggtagg
      241 gcatgactgt ccaataggga agggcggcaa tgccacgtct gctggcaacc agttggataa
      301 tggcgatggt cagctcgctg tgccggcgcg gcgggttgat gtttttcgga atcaggattt
      361 tcttgggcaa atccagcatt tcatcgggaa cgggataggt aatcagggtt tccccgataa
      421 agtcttccgc cgtccaaacg tttttggcgg caagcggatg gtccggggcg caaatgccga
      481 ccatttcgta ggcaaacagt gggtggaagc tgatgctgct ttgtttttcg gcttcggaga
      541 caatggcgag gtcggcgcgg tgttgcagca gcagcccgac gggatccgct tggaatcctg
      601 atacgatatc cagttcgact tggggccaca tcgggcggaa ttcgcccatg gcgggcatca
      661 gccagtcgaa acaggtatgg cattcgacgg caatccgcag ctctcccgcc tccccttccg
      721 tgattcgcgc caaatctcgt tctgcaacag caacttgagg tatcagttcg tgggcgaggc
      781 gcagcagcct ttcgcccacc ggggtaaagc gcaggggcgt ggatttgcgt tcgaacagcg
      841 gcgtgccgta gtggttttcg agcatacgga tctggtggga aagggcggat tgggtaagga
      901 aaacccgttt ggcggcaagg gagacgctgc cggtttcttc aagtgccagc agggttttga
      961 ggtggcgcaa ttcgataatg gaatccatgg ggcttcagac ggcatattga accggcgcat
     1021 attaaaataa ttcatatgcg ggtgcaaatc ggaaaaatgg gcgtttcggg catttgcggt
     1081 tagaatgccc gcttgtttgg aaatgacagg aaaaatatgg ttttgcgcag agatttttta
     1141 gcctggtgcg acgaaacgtt gcagacggca tcgtttttta aagattacgc ccccaatggt
     1201 ttgcaggttg aagggaggga atatatcggg aaaatcgtta cgtcggtaac ggcaagcagg
     1261 gcagcgattg attttgctgt ggagcagaag gcagatttgc ttttggtaca tcacggtatg
     1321 ttctggaaaa acgagctgcc gaccgttacc ggttggaaaa aagaacggat tgccgcactg
     1381 ttacggcacg acatcaatat ggcaggctac catctgcccc tggatgtgca ttccacactg
     1441 ggcaacaatg cccaactcgc cgacagattg ggttttgcga cagaaaaacg gttcggcgaa
     1501 caaaacctgc tcaactcggg cagcctgaaa caagccaaga cactcggcgc attggcggcg
     1561 catattgaaa cagttttgca acgtaagcct gtcgccatcg gcaatcccga acgcgaaatc
     1621 cgacgggttg catggtgtac cggcggtgca cagggctttt ttcagacggc aatagatgaa
     1681 ggcgtcgatc tgtatctgac gggtgaaatt tccgaagccc aataccacct cgccaatgaa
     1741 acgggtacgg ctttcatttc ggcagggcat cacgcgacgg aacgttacgg cgtacgcgcg
     1801 ctggcagaat cggcggcaga ggttttcggg ttggaagtgt gccattttga cgaaaacaac
     1861 ccggcttgag tcttgagaaa tatcataaaa ctttacctta ttttaaataa tgctttgagt
     1921 atcactgcaa actgggtaaa attgcaatgt ttaatggctc ggcattccca aaatattagg
     1981 acgactgaat aatggataat caagaaatca acaacggccg ccgccgtttc ctgacacttg
     2041 cgacctgcgg cgcgggcgga gtggcagcat tgggtgtggc aacgccgttt gtggccagtt
     2101 ttttcccttc ggaaaaagcc aaggccgccg gtgctgccgt cgaagtggat gtcagtaaaa
     2161 tcgaagcggg tcagctgctg accgccgagt ggcaaggcaa accgatttgg gtgctcaacc
     2221 gtacagatca gcagcttaaa gacctgaaag gcctgaacgg cgaacttacc gatcccaatt
     2281 ccgatgcgga acagcagccg gagtatgcta aaaacgagac ccgttcgatt aagccgaaca
     2341 tccttgtcgc catcggtatc tgcacccatt tgggctgctc gcccaccttc cgtcccgaca
     2401 ttgcccccgc cgatttgggt gcagactgga aaggcggctt cttctgcccc tgccacggtt
     2461 cgaaattcga cttggccggc cgcgtatata aaggtgttcc tgccccgacc aacctggttg
     2521 tcccgccata taaatacttg agcgacacaa ctatcttggt gggcgaagac taagaataag
     2581 gaacgataat tatggcaaac caaaccaata gcaaagcaaa agcattgtta ggctggatgg
     2641 acgctcgctt ccccctgtct aaaatgtgga atgagcattt ggcgcaatac tatgcgccga
     2701 aaaactttaa tttttggtac tatttcggct ctttagccct gctcgtcctc gtgattcaaa
     2761 tcgtcagcgg tattttcctg acgatgaact acaaaccgga cggcaacctg aatgcctacc
     2821 atctgcctgc tgcctttacc gcagtagagt acatcatgcg cgacgtgtcc ggcggttgga
     2881 ttatccgcta catgcactcc accggcgcat ctttcttctt cattgttgtt tatctgcaca
     2941 tgttccgtgg tttgatttac ggttcatata aaaaaccgcg cgaattggtg tggattttcg
     3001 gttctctgat tttcttggca ttgatggcgg aagcctttat gggctacctg ctgccttggg
     3061 gtcagatgtc cttttggggt gcgcaggtga ttatcaacct gttctccgcc attcccgtca
     3121 tcggtcctga tttgtccact tggattcgcg gcgacttcaa cgtttccgac gttaccctga
     3181 accgcttctt cgccctgcac gttatcgccg taccgctggt attgctcggt ttagtcgtgg
     3241 cacacatcat cgcactgcac gaagtgggct ctaacaaccc cgacggtgtg gaaatcaaga
     3301 aaaacaaaga tgaaaacggc attccgcgcg acggcatccc gttccaccct tactacactg
     3361 ttaaagacat cctgggcgtc gttgtattcc tgattgtctt ctgtgccgta ttgttctttg
     3421 cccctgaagg cggcggctac ttcctggaag cgccgaactt cgatgcggca aatgcgctga
     3481 aaacaccccc gcacattgcg ccggtatggt atttcacacc gttctacgcc attttgcgtg
     3541 cgattccttc cttcttggga acgcaggttt ggggtgtaat cggtatgggc gcggcggtcg
     3601 tgttgattgc cctgctgcct tggctggaca ggggcgaggt gaaatccgtc cgttaccgcg
     3661 gtccgatttt caaaaccgca ttggtcttgt tcatcatttc ctttatcggt ttgggtattt
     3721 tgggcgcgat ggtggcaacc gatacgcgta ctttggtcgc gcgcatcctg tccttcgtct
     3781 atttcgcatt cttcctgggt atgccgttct ataccaagct ggataaaaac aaaccggttc
     3841 ccgaacgcgt aaccatgagc accgccaaac aaaaaatcat gttctttgtt tacgtcggca
     3901 ttaccgccgt gggtgcttac ctgtttgcaa ccaacatctg atgagggcag tcaaaatgaa
     3961 acaagctatg aaaaattggt ttgctgcctt gctgtcggca gtacccatgg gtgcggcatt
     4021 tgcttcgggc ggtcatgcac actatgagaa agtcgatatc gatttgcgcg accaagtcag
     4081 cctgcagcgc ggcgcacaaa tctttaccaa ctactgtttg tcctgccact cggcaagcgg
     4141 tatgcgtttc aaccgtttga aagacatcgg tttgactgac gaagaaatca agaaaaacct
     4201 gatgtttacc accgataatg tcggcgatgt catgcattcg gcgatgaacc cgaaagatgc
     4261 ggcaaaatgg tttggtgctg ctccgcccga tttgacattg attgcgcgtt ccaaaggtgc
     4321 agactacctt tacgcttata tgcgcggctt ctataaagat ccgacccgtc cgagcggctg
     4381 gaacaatact gtattcgata aagtcggtat gccccacccg ttgtgggaac agcaaggcgt
     4441 tcaagccgtt gagttggatg ccaaaggtca gccggttatg gtaaaagacg aacacggcga
     4501 gatgaagcct aagctgtatt gggaatctac cggtttgcac agccgccgtc tgcctaacgg
     4561 caaagtgatc cagaaagagt acgacgcata tgtacgcgat ttggtcaatt accttgtgta
     4621 catgggcgaa cctgcacaac tgcaacgcaa acgtataggc tatgtcgtga tgattttcct
     4681 atttgcggtt atgctgcctt tggcgtattt cctgaaaaaa gaatattgga aagacgtaca
     4741 ctaagcgttt ggaacaaaag ggcaaatcct ttagggtttg cccttttttc attttgcctg
     4801 ccgtttgaaa agcctgaatc cgtatgccgt ctgaacatag ctgcaacatt tcagacggca
     4861 tcattctaaa aatgtcaggc atcgggaact taatcagggt ttgtggcaga ttgatattga
     4921 attaggaaaa ggtctgaaac cgcaagcagc atggtaaggc gatgtcgaac aggtttaaag
     4981 ccaatccgct atattttttg gagctgcacc gtgcaaacag gtagaatggc ggcggttttg
     5041 tccggtgtga aaatgtttga atattaggat gattgatgga gctgatgact gttttgctgc
     5101 ctttggcggc gttggtgtcg ggcgtgttgt ttacatggtt gctgatgaag ggcaggtttc
     5161 agggcgagtt tgccggtttg aacgcgcaat tggcggaaaa ggcggcaaga tgtgatttta
     5221 ttgaacaggc acacgcagaa attgcatcag aattggccgc tttggatggg aaatacgagc
     5281 atttgcagga cgaaaattat gctttgggca accgtttttc cgcagccgaa aagcagattg
     5341 cccatttgca ggaaaaagag gcggaatcgg ttcggttgaa gcagtcgtat atcgatttgc
     5401 aggaaaaggc gcagggtttg gcggttgaaa acgaacgttt ggcaacgcag ctcgggcagg
     5461 aacggaaggc gtttgccgag caatatgcgc tggaacgcca aatccgccaa agagtcgaaa
     5521 ccgatttgga agaaagccgc caaactgtcc gcgacgtgca aaacgacctt tccgatgtcg
     5581 gcaaccgttt tgccgcagcc gaaaaacaga ttgcctattt gcaggaaaaa gaggcggaag
     5641 cggagcggtt gaggcagtcg cataccgagt tgcaggaaaa ggcgcagggt ttggcggttg
     5701 aaaacgaacg tttggcaacg caaatcgaac aggaacgcct tgcttctgaa gaaaagctgt
     5761 ccttgctggg cgaggcgcgc aaaagcttga gcgatcagtt tcaaaatctt gccaacacga
     5821 ttttggaaga aaaaagccgc cgttttaccg agcagaaccg cgagcagctc catcaggttt
     5881 tgaacccgtt gaacgaacgc atccacggtt tcggcgagtt ggtcaggcaa acttatgata
     5941 aagaatcgcg cgagcggctg actttggaaa acgaattgaa acggctccaa gggttgaatg
     6001 cgcagctgca cagcgaggca aaggccctga ccaacgcgct gaccggcacg cagaacaagg
     6061 ttcagggcaa ttggggcgag atgattctgg aaacggtttt ggaaaattcc ggccttcaga
     6121 aagggcggga atatgtggtt caggcggcat ccgtccgaaa agaggaagac ggcggcacgc
     6181 gccgcctcca gcccgacgtt ttggtcaacc tgcccgacaa caagcagatt gtcattgatt
     6241 ccaaggtttc gctgacggct tatgtgcgct acacgcaggc ggcggatgcg gatgaggcgg
     6301 cacgcgaact ggcggcgtat atcgccagca tacgcgcgca tatgaaaggc ttgtcgctga
     6361 aggattacac cgatttggaa ggtgtgaaca cattggattt cgtctttatg tttatccctg
     6421 tcgaaccggc ctacctgctg gcgttgcaga atgacgcggg cttgttccaa gagtgtttcg
     6481 acaaacggat tatgctggtc ggccccagta cgctgctggc gactttgcgg acggtggcga
     6541 atatttggcg caacgagcag caaaaccaaa acgcgctggc gattgcggac gaaggcggca
     6601 agctgtacga caagtttgtc ggcttcgtac agacgctcga aagcgtcggc aaaggcatcg
     6661 atcaggcgca aaacagcttt cagacggcat tcaagcaact tgccgaggga cgcgggaatc
     6721 tggtcggacg cgccgagaaa ctgcgtctgt tgggcgtgaa ggcaggcaaa cagcttcaac
     6781 gggatttggt cgagcgtgcc aacgaacaga cggcatttgc tttgggcaaa ccttcggaac
     6841 aggaagcggc tgacgaagcc gaataggaaa ggcggatgcg tttcagacgg catcagtgca
     6901 gatgatgccg tctgaagact ttgggaagtc ggttagaaaa acggattgtg ccgtttttcg
     6961 tgtccgatgg aagtcatacg cccgtgtccg gcgacaactt gcacggtttc ggggaaggcg
     7021 aataatttgt tgcggatatt gttgattaag tcggcgtggt tgccgcgcgg aaaatcggtc
     7081 ctgcctatgg tttcgtaaaa cagcacgtcg cccgcaatca gcagctccgc ttcggcacaa
     7141 tagaaaacca cgtgtccggg cgtgtgcccc ggaatatgca gcacttggaa ggcatagcgt
     7201 ccgaccgtga gcgtttcgcc ttcttcgagc caacggttcg gcgcaaaggc gggcgagacg
     7261 gggaatccgt attgcgcggt ggtttgcggc agcgattgga gcaggaattc atcttcgcgg
     7321 tgcgggccga ggacggggac tttatgcgtt tccagcatct cgaccacgcc gcccgcgtga
     7381 tcgagatgac cgtgcgtcag ccagattgcc gtgagcgtga gtttgcggtt tgccaacgct
     7441 tgcagcagga acggcacgtc gccgccgaca tcggtcagga cggcttcgcc gctttcgtcg
     7501 tcccaaatca gggtgcagtt ttggcggaag ggggtaacgg ggagaatttc gtaacgtagg
     7561 gtcatcggcg tgttcctaaa acggtttttc agacggcatc gggtttgccg tttgttttat
     7621 ggcggtttgc cgcctgtttt gatattgggg gaatcaggtg attaagaaga taatcggcgg
     7681 catcataccg atttttacgg cggttttcat ccctgcatcg gcaggcgcgg cggatttgat
     7741 gctggcgcag gaatacaaag ggcaggacat tgccggctgg gcgatgagcg agaagctcga
     7801 cggcgtgcgc gcgtattggg acggaaagca cctgataagc cgtcagggtt atgcgtttac
     7861 gccgcccaaa ggctttaccg cgcagtttcc gccttatcct ttggacggcg aattgtatag
     7921 cgggcgcggt cagttcgagc agatttccgc taccgtgcgt tctgtttctt cagactggcg
     7981 cggtatccgc ctgcacgttt tcgacgtacc caaggcgcag ggcaacctct accaacgttt
     8041 ggcagtcgca acgcagtggc tgaaaacgca tccgaacgcg ccgattacca tcatcccgca
     8101 aatcaaagtg cgcgaccggc ggcacgcgat ggacttttta aaacaaatcg aagcgcaggg
     8161 cggcgaaggc gtgatgctgc gccagcccga atcccgttac agcggcggca ggagcagcca
     8221 attattgaag ctgaaaagcc aatacgacga cgaatgcacg gtaacgcggc actatgaggg
     8281 caaagggcga aacgccggac ggctgggcgc ggtcggctgc aaaaaccgac acggcgaatt
     8341 ccgcatcggc agcggtttca aggacaaaga ccgcgacaac ccgcccaaaa tcggcacgct
     8401 gattacctac cgttaccgtg gctttacgcg gaaaggcacg ccgaaatttg ccacatttgt
     8461 gcgcgtgcgt accgaccgtt gagcaggacg gctcggacgg catccgatac tttggtttat
     8521 aattttcctt ttaccgaccg attccgacat atgaccgatt tagaaaccaa acgccttgaa
     8581 acacaggcga tgcttgaaaa cgccgatctt ttgttcgacc aaggccaatg ccgtgccgca
     8641 ctgcaaaaag tggcggacga gattacgcgt gatttggggg ataaatatcc gttgctgctg
     8701 cccgtgatgg gcggcgcggt ggtgtttacg gggcagttgc tgccgctgtt gcgttttccc
     8761 ttagattttg attatgttca cgtttcccgt tacggcgaca agctggaggg cggcgcgttc
     8821 aactggaagc gtatgcccga tgcggaacaa atccggggca ggcacgtggt cgtgttggac
     8881 gatattttgg acgaagggca tacgatgtcc gccattcaag ccaaactttt ggaaatgggt
     8941 gcggcaagct gccgtgcggc ggtgttcgcc aataaattaa tcgacaaaga aaaaccggtc
     9001 aaagccgatt atgtcggact ggatgtgccg aaccgttatg ttttcggtta cggcatggat
     9061 gcggcaggct gctggcgcaa tttgggcgag atttacgcat tgggcggaaa ataagggatg
     9121 cgatgccgtc tgaaggcctt tcagacggca tcgcgcccat acgccggcag gattaatgag
     9181 gaacaggacg catcaatatg atagggcttt taatcatcac acacgaaacc ataggcgaag
     9241 cctaccgcaa gctggcgcat catttttttc cgggcgggct gcctgaaaac gtccgcatac
     9301 tcggcgtgca gccgacggaa ggccaagacg acattatcaa caacgccatt tccgcgctgc
     9361 aagagtttcc tgaaaaccac ggtgtattga ttatgaccga catcttcggc gcgaccccct
     9421 gcaatgccgc ccgccggctc gtgcgcgaaa acaaatcggc gattttgacc gggctgaacg
     9481 cgccgatgat gattaaggcc gtccaatatt cgccggcggc ggaagacctt gccgccttta
     9541 ccgaatgcgt cagagaggcg gcggtaaaag gcattttcgc catcacgtcc gcgcccgaag
     9601 atttggtgtg ccggcgcagc ggcgatgccg tctgaagaag cggcagggca ggaaaacatt
     9661 ttaaacggcg tctgctgccg atatatagcg gattaacaaa aatcaggaca aggcggcggg
     9721 ccgcagacag tacaaatggt acggaaccgg ttcgcccggt gcttcatcac cttagggaac
     9781 cgttcccttt gggccggggc ggggcaacgc cgtactggtt tttgttaatc cactatacat
     9841 aacacgggaa ccgaaatgct caaacaatcc atcgaaatca tcaacaaact cggactccac
     9901 gcccgcgcgt ccagcaagtt cacccaaacc gcgtcccaat tcaaaagcga agtctgggtt
     9961 acgaaaaacg gcagccgcgt caacggcaaa agcattatgg ggctgatgat gctcgcagcc
    10021 gccaagggta cggtcatcga actggaaacc gacggcttgg acgaagccgc agccatgaag
    10081 gcattgaccg acttaatcaa cgactacttc ggcgagggcg aataatgagt atcgtgctgc
    10141 acggcgtggc ggcgggcaaa ggcattgccg tcggttgcgc ccacctgatt gcgcgcggta
    10201 cggaggaagt gccgcagtat gatgttgctc aggcggacac cgatgccgaa gccgaacgtt
    10261 tcgatgccgc cgtcaaagcc acgcgcaaag agttggaaca gctccgcagc gcgattcccg
    10321 aaaacgcccc gaccgagttg ggcgcgttca tctcgctgca cctgatgctc ttgaccgatg
    10381 ttaccttgtc gcgcgaaccc gtcgatattt taagggaaca aaaaatcaac gccgagtggg
    10441 cattgaagca gcagagcgac aaactcgccg cccaattcga caatatggac gatgcctatt
    10501 tgcgcgaacg caagcaggat atgctgcaag tcgtccgccg catccacaac aacctgatcg
    10561 ggcagggcaa cgagttggag gttgccgaca acctgtttga cgaaaccgtt ctgattgcaa
    10621 acgacctttc acccgccgac acggttttgt ttaaagagca gcgcattgcc gccttcgtta
    10681 ccgatgccgg cggccccacc gggcatacgg cgattttggg caggagcttg gacatcccgt
    10741 ccgtcgtcgg gctgcacaac gcgcgcaaac tgattaccga aggcgaaacg gtcatcgtgg
    10801 acggcatcaa cggcgtgttg attatttcgc cggatgagtc ggtgttgaac gaataccgcc
    10861 gccgcgcccg cgaataccgc agccacaaac gcgatttgaa caagctcaaa aaaaccgccg
    10921 ccgctaccgc cgacggggtc tgcatcgagc ttgtgggcaa tatagaatcc gccgaagacg
    10981 tgaaaccgct gcacaacctc ggcgcagacg gcatcgggct gttccgcagc gagtttcttt
    11041 acctgaaccg cgatacgatg ccgtctgaag acgagcagta cgaagtgtac agcgcgattg
    11101 tcaaaaaaat gaagggcaaa agcgtaacga tacggacagt cgatttgggt gtggacaaaa
    11161 atccgcgctg gttcgggaaa aacagcacgc ccaacggcag cctcaacccc gcgctgggca
    11221 tgaccggcat ccgcctgtgc cttgccgaac cggtcatgtt ccgcacgcag atgcgcgcca
    11281 tcctccgtgc ggcggtacac ggtcccgtcc gcatgatgtg gccgatgatt acctccgtat
    11341 ccgaagtgcg ccagtgcctc atccacctcg acaccgcgca acgccagctt gccgaacgcg
    11401 gcgatgcctt cggcgaagtc ggcatcggct gcatgattga aattccgtct gccgcgctga
    11461 ccgtcggcag tattttgaaa ctggtcgatt tcatctccgt cggtaccaac gatttgatcc
    11521 aatatattct gtccgtcgat cgcggcgacg acagcgtcag ccatctctac caacccggac
    11581 atccttccgt gctgaaaatg ctgcaacacg tcatccgtac cgccaaccgc atggacaaag
    11641 acgtgtccgt atgcggcgag atggcgggcg ataccgcgtt tacccgcgtt ttattgggta
    11701 tggggctgcg ccgtttttcc atgaatccga acaacatcct gcccgtcaaa aacatcattc
    11761 tgcacagcaa tgccggacag ctcgaaggcg atattgcgaa agtcatccgc tgcgaagacg
    11821 aagagaagtc ggaaaagctg atcaaacaga tcaacagcgt gtctgtcgcg gaagaagccg
    11881 aagtcaaggg acggaaataa atattgttga caatgcgagc gggttgcagt caaataacgc
    11941 ccttaaaaaa tacagctgct ttggtcaaga aatgccgtct gaaaaatttt cagacggcat
    12001 tttgtttggc aggcatttct gccgcatcat cggaaacgga cgatacgccc ttcatcgacg
    12061 cggatgcgga ccgtaccgtt tccggaaata ccgtccgtat gttgtccgac agtcaggttt
    12121 aaggtcagct cgccgtgttc gggatggacg gcggaaagcc ggagcgagtc gggcaggcgg
    12181 acgagggaca gcagacggca ttccgttcca tgattgtcca agcacacggc attttgcgga
    12241 atatggcggt catcgtcggt attgggcagc cccatcagac gggcgacctg cacgccggca
    12301 ggcgtttgaa tcaaggtttc gggcgtaccg cattgaagga ttttcccctc gtgcatgacg
    12361 gcgatttcgt ccgccgccgt gcaggcctct tcgggcgaat gcgttaccaa aacggcaggg
    12421 atgccgccct tgcggatgcg ttcggcggtc atacggcgca gccggtcgcg caaatgcgtg
    12481 tccaaactgg aaaacgattc atccagcaac agcagggaag ggcggacaac caaagcgcgc
    12541 gccagtgcca accgttgctt ctcgcctccg gaaagttttt caggcttgcg gtgcgcctcg
    12601 ttttccagcc cgacttcggc aagtgccgac aaggcgaggc gttcggcttc ggctttcggc
    12661 attttttgca ttttcaaacc gaatgccgta ttttccagcg cactcatatg gggaaacagc
    12721 gcgtaatctt gaaacatcag cgagatacgg cgtttttcgg gcggcataca ggtaatgttt
    12781 tccccgttca gccgaatttc gccgccgtcc ggccggacga tgcccgcaat catattcagc
    12841 agggtggatt tgccgcagcc cgaccgcccc agtacggcga gtattttgcc gcgcccgaca
    12901 gtcaggcaga tgttgtcggc gaccgttttg ccgccgaagc atttgcagag tccgttcagt
    12961 tcaagcatgg cgcatcctat aaacgtatgc cgtccagcca ctcggacagc ggatgggcgg
    13021 catcaatcag accgtaacgg taaaacgcgt ccagcgtaac cagttgcgcg tcgtgcatca
    13081 tgtttttcga caacatggca tccaataggc cgccaatgtc catcttttca aaacccgcta
    13141 cctcgccatc ctgattttca ggcaggaagg tttcgggcag gacggcatcg aacacataca
    13201 ggatttcatt gtgcacacct cggctgacgg ggcgaaggct gtgcagccgc gatactgggc
    13261 ggatgagcgg aaacagcgtt ttatccaaac cggcttcttc gctgctttcg cggcacacgg
    13321 cttcagacgg catttcgccg ccggaaacac cgccgccggc aatattgtcg agcttgccgg
    13381 gatcgactgc tttgtgcgga ctgcgcctgc ctatccaaaa atgccatctg ccgttcgatt
    13441 cgaccaaacc gttgagatgg acggcgcggc tgagtagtcc gaacggacgg aaagcggcgc
    13501 gttcgagcgt gaacaagggg ttgccgccgc cgtcggtcag gtcgaaacac tcgttgcgcc
    13561 atccgtgaag cagccccgcc ttgttccatg tgcgggcgag gtgctgcaag cgtccgccca
    13621 tatccggcca gccgtccgca ttcagaaaaa tgccgtctga agactcggag cagcctgcct
    13681 cccagtcttt tttgatgcgc tcagcccatt ccggcgacag attgcccaaa ggaagacggt
    13741 tcagatacag cgttttccag caactttcgg caccgtaact tgcttttgcc cgctcgaaca
    13801 gggcatcaag gtcttgtttg ctgacggatt cggtaaaacg gacggtcggc atagccggct
    13861 ttcttgaaca caatgcggaa gattgtagcc aaaccgcctg aatttgcaac cgcaaacaga
    13921 cggcggcctg ccgttccgat attgcggcag gaaggaaaat ccggcaaaaa acggaggcgg
    13981 caggaaacag gaaaaacaaa accccctcgg cattgaaccg aggggtttga tatttggttg
    14041 cgggagcagg attcgaacct acgaccttcg ggttatgagc ccgacgagct accatgctgc
    14101 tccatcccgc gtcagaagat gaaactatac ggcagatttt ttatgttgtc aaacattatt
    14161 tccgtgaaaa atcataaagt tttccgtccg gcggcttatg cctgatttga aatatcattt
    14221 tctttacaaa agttcatgtt cgtgatttaa ttttggttaa catcgaaaca ggggtgctgc
    14281 ctgatgttca ggcggctgag aaataccctt tacacccgat cgggataata cctgcgtggg
    14341 gagttttcac ggattctgtc tttcagacgg cattggtttt caaatgccgt ctgaaaacgc
    14401 aaaacgctcc tgtttcttta ttctaaacga gaaaacagga gcatttttta tgactacgcc
    14461 aaaaaaaacc gccaaaattt ccggcaacga agcgcgcgaa ctttccgact tgagcgaaga
    14521 catcggcatc cgcttcaaat atcagaactc cgaacgcgtg tatctgcaag gcagccgcga
    14581 cgacatccgc gtacctttgc gcgaaatccg tcaggacgac acctacacgg cgcaaggtac
    14641 ggaagccaac ccgcctattc cggtgtacga caccagcggc gcatacggcg atccggcggc
    14701 gcacatcgac ctgaaacaag gcctgccgca catccgcacg gcgtggctgg acgaacgcgg
    14761 cgataccgaa atcctgccca agctctccag cgaatacggc atcgaacgtg cgcacgatcc
    14821 gaaaaccgcc catctgcgct tcaaccaaat cacccgcccg cgccgtgcca aagccggccg
    14881 caatgtaacc cagcttcact acgcgcgcca aggcattatc acgcccgaaa tggagttcgc
    14941 cgccatacgc gaacgcatga agctggacga gcttttcaga cggcctgaat acgccaagct
    15001 cttgaaacag cacacagggc aaagtttcgg tgcgaacatc ccgacccgtc ccgaccaaat
    15061 cacgcccgaa ttcgtgcgcc aagaaatcgc cgccggccgc gcgatcatcc ccgccaacat
    15121 caaccacccc gaactcgaac cgatgattat cggccgcaac ttccgcgtca aaatcaacgg
    15181 caacttgggc aattccgccg taacctccag cctgaccgaa gaagtcgaaa aaatggtgtg
    15241 gtcgctgcgt tggggcgcgg acacgattat ggacttatcc acaggcgcgc acatccacga
    15301 aacgcgcgaa tggattatcc gcaacgcgcc cgtccccatc ggcacagtgc cgatttacca
    15361 aactttggaa aaaaccggcg gcatcgccga agatttgact tgggatttgt tccgcgacac
    15421 cttaatcgag caggcggaac aaggcgtgga ctatttcacc atacacgcgg gcgtgttgct
    15481 gcgttatgtg ccgatgaccg ccaaccgcct caccggcatc gtatcgcgcg gcggttcgat
    15541 tatggcgaaa tggtgtttgg cacatcatcg ggaaaacttc ctctacacgc atttcgacga
    15601 aatctgcgaa atcatgaagg cgtatgacgt atcgttcagc ttgggcgacg gcctgcgccc
    15661 cggctgcatt gccgatgcca acgacgaatc ccaattcgcc gaattgcaca ccttgggcga
    15721 attgaccgat aaagcgtgga aacacgacgt acaagtcatg atcgaaggcc ccggccatgt
    15781 gccgctgcaa cgcgtcaaag aaaatatgac cgaagagttg caacactgct ttgaagcacc
    15841 gttttacaca ctcggcccgc tcgttaccga catcgccccc ggctacgacc acatcacttc
    15901 gggcataggc gcggccaata tcggctggta cggcacggcg atgctttgtt acgttacccc
    15961 caaagaacat ttggggctgc ccgacaaaga agacgtgcgc accggcatca tcacctacaa
    16021 actcgccgcc cacgccgccg acctcgccaa aggctggccg ggcgcgcaat tacgcgacaa
    16081 cgccctgagc aaggcgcgtt tcgaattccg ctggcgcgac caattccgct taagcctcga
    16141 ccccgaacgc gccgagagct tccacgacga aaccctgccc gccgaaggcg cgaaaatcgc
    16201 ccatttttgc tcaatgtgcg gccccaaatt ttgctcgatg aaaatcacgc aggaagtgcg
    16261 cgactacgcc gacaagcaaa aagcccaacg gcagggtatg gaggaaaaag cggtcgagtt
    16321 cgtcaaaaaa ggcgcgaaga tttacagttg aaacgtcaag caaaaaatgc cgtctgaagc
    16381 ccggaaaaaa ggcttcggac ggcattcttt cgcttgtgaa aatgattaaa caaccgatca
    16441 ttaaagtaat ttttaaaatt tttatattca catagatggg aatgatggga tttagggttc
    16501 tgattttgtt ttgagagaat gaaggaattt gagattgtgg gcgattatcg ggaaaaatag
    16561 aatctttc
//
LOCUS       DAXFWM010000053        16463 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000053 DAXFWM010000000
VERSION     DAXFWM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16463
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.053"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            153..1217
                     /locus_tag="ACFI8B_001567"
     CDS             153..1217
                     /locus_tag="ACFI8B_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC3834749.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIRVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="ACFI8B_001568"
     CDS             1358..1681
                     /locus_tag="ACFI8B_001568"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC3834750.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKSEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="ACFI8B_001569"
     CDS             1755..2078
                     /locus_tag="ACFI8B_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC3834751.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="ACFI8B_001570"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="ACFI8B_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC3834752.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="ACFI8B_001571"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="ACFI8B_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC3834753.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="ACFI8B_001572"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="ACFI8B_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC3834754.1"
                     /translation="MSETQNEQTVQLLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4426
                     /locus_tag="ACFI8B_001573"
                     /pseudo
     CDS             3845..4426
                     /locus_tag="ACFI8B_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
     gene            4461..4814
                     /locus_tag="ACFI8B_001574"
     CDS             4461..4814
                     /locus_tag="ACFI8B_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC3834755.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFNAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4804..6731
                     /locus_tag="ACFI8B_001575"
                     /pseudo
     CDS             4804..6731
                     /locus_tag="ACFI8B_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
     gene            6807..7484
                     /gene="misR"
                     /locus_tag="ACFI8B_001576"
     CDS             6807..7484
                     /gene="misR"
                     /locus_tag="ACFI8B_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC3834756.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7497..8903
                     /gene="misS"
                     /locus_tag="ACFI8B_001577"
     CDS             7497..8903
                     /gene="misS"
                     /locus_tag="ACFI8B_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC3834757.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9021..11444
                     /locus_tag="ACFI8B_001578"
                     /pseudo
     CDS             9021..11444
                     /locus_tag="ACFI8B_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11489..11734
                     /gene="rpsP"
                     /locus_tag="ACFI8B_001579"
     CDS             11489..11734
                     /gene="rpsP"
                     /locus_tag="ACFI8B_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC3834758.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11750..12259
                     /gene="rimM"
                     /locus_tag="ACFI8B_001580"
     CDS             11750..12259
                     /gene="rimM"
                     /locus_tag="ACFI8B_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC3834759.1"
                     /translation="MTDAQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12259..13008
                     /gene="trmD"
                     /locus_tag="ACFI8B_001581"
     CDS             12259..13008
                     /gene="trmD"
                     /locus_tag="ACFI8B_001581"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC3834760.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAKL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13023..13388
                     /gene="rplS"
                     /locus_tag="ACFI8B_001582"
     CDS             13023..13388
                     /gene="rplS"
                     /locus_tag="ACFI8B_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC3834761.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13709..14434
                     /locus_tag="ACFI8B_001583"
     CDS             13709..14434
                     /locus_tag="ACFI8B_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC3834762.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14469..15344
                     /locus_tag="ACFI8B_001584"
     CDS             14469..15344
                     /locus_tag="ACFI8B_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC3834763.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVIFLILVVMNLVSGFQALGILMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15421..16335
                     /locus_tag="ACFI8B_001585"
     CDS             15421..16335
                     /locus_tag="ACFI8B_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC3834764.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
BASE COUNT         4270 a         5267 c         3634 g         3292 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcagag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccgcc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcattaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagggcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagagcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaattgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg caccgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaact tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caactgcttg tcgaacacct catcgagctg cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgcgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc cttatccgcc
     4441 cttaaaccgt aaagtccgcc gtgaacagac aaaccgctta cttccttgcc tccttcagcc
     4501 tgattgcact gattgcccta tccctttctt gggaattatg gattgcaccg ttacgcccgg
     4561 gcggttcgtg gctcgcgctc aaagccctgc ccctctgcct gccgctttca ggtattctaa
     4621 agaaaaaaat ctatacctac caatacagct ccatgctggt tctgatttac tttgccgaag
     4681 ccgtcatgcg cctgttcaac gcctatcccg cagaaaaaat ttgcgccgcg ctttccgcag
     4741 tattcagcat catcttcttc atatcctgcc tgtcgttcgt caaacaatac aaggaaacaa
     4801 acaatgtccg ctgaaacgac cgtatccggc gcgcgccccg ccgccaaact gccgatttac
     4861 atcctgccct gcttcctttg gataggcatc atccccttta ccttcgcact caggctgaaa
     4921 ccgtcgcccg acttttacca cgatgccgcc gccgcggccg gcctgattgt cctgttgttc
     4981 ctcacggcag gaaaaaagct gtttgatgtc aaaatccccg ccatcagctt cctcctgttt
     5041 gcaatggcgg cattttggtg gcttcaggca cgcctgatga acctgattta tcccggaatg
     5101 aacgacatcg cctcttgggt tttcatcttg ctcgccgtca gcgcgtgggc ctgcaagagt
     5161 ttggtcgcac actacggaca agaacgcatc gttaccctgt ttgcctggtc gctgcttatc
     5221 ggctccctgc ttcaatcctg catcgtcgtc atccagtttg ccggctggga aaacaccccc
     5281 tgcttcaaaa catcatcgtt cacagagggc aaggcgtaat cggacacatc gggcagcgca
     5341 acaacctcgg acactacctc atgtggggca tactcgcctc cgcctacctc aacggacaac
     5401 gaaaaatccc cgcagccctc ggcgcaatct gcctgattat gcagaccgcc gttttaggtt
     5461 tggtcaattc gcgcaccatc ttgacctaca tagccgccat cgccctcatc cttcccttct
     5521 ggtatttccg ttcggacaaa tccaacagac ggacgatgct cggcatagcc gcagccgtat
     5581 tccttaccgc gctgttccaa ttttccatga acgccattct ggaaaccttt acaggcatcc
     5641 gctacgaaac tgccgtcgaa cgcgtcgcca acggcggttt cacagacttg ccgcgccaaa
     5701 gcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg cacggctgga
     5761 acagttttgc ccaacaaacc ttcctgatca atgccgaaca gcacaccata cacgacaact
     5821 tcctcagcac cttgttcacc cattcccaca acatcatcct ccaactcctt gcagaaatgg
     5881 ggatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc gggctgctga
     5941 aacgctccct gacccccgca tcacttttcc tgctgtgcgc gcttgccgtc agtatgtgcc
     6001 acagtatgct cgaatatcct ttgtggtatg tctatttcct catccccttc ggactgatgc
     6061 tctttctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc gccaatctcg
     6121 gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac tggacataca
     6181 cccggctggt taactccttt tcccccgccg ctgacgacag tgccaaaacc ctcaaccgga
     6241 aaatcaacga actgcgctat atttccgcaa acagcccgat gctgtccttt tatgccgact
     6301 tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg gcggaagaag
     6361 caaccctcaa agcactaaaa taccgcccct actccgccac ctaccgcatc gccctctact
     6421 tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcaaca cagtcctatt
     6481 acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta tgggcaccgc
     6541 tgctgcccga actgctcaaa gactgcaaag ccttcgccgc cgctcccggc catccggaaa
     6601 caaaaccctg caaacgaccc gcgtcggcgg atgcggatac cgcccgaaat gtaaagctcc
     6661 atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa aaaacattgc
     6721 agccgtgtta agataaactg tcaaacaatc ttttcacgcc acgcccgaaa cagggtcggg
     6781 gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat gacgatgccc
     6841 tgctgaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc cgcagcgttc
     6901 ccgacgggga agcaggcgta caggaaatcc tgagcgggca atacgatgta gtcgtattgg
     6961 attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc gcccgaagca
     7021 ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc atcggcttgg
     7081 aaatgggtgc ggacgactat gtccccaaac cctgcacacc acgcgaactc ttggcacgca
     7141 tcaatgccat cctccgccga gcacaacaca gcggcgaaca gaacaacgca cccaacagca
     7201 tctccgtcag cgatgtcgtc ctataccccg ccaaacgcca ggcatccgtc aaagacatgc
     7261 cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc catgccggac
     7321 aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaaactg gcaaaattcg
     7381 accgcagtat cgacgtacac atctccagca tccgccacaa gctgggcgat gcctccctga
     7441 ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaagcagc gggcaaatga
     7501 aactgttcca acgcattttc gccacatttt gcgcggttat cgtctgcgca atctttgtgg
     7561 cgagtttttc tttttggctg gtgcagaaca cccttgccga aaaccaattc aaccaacgcc
     7621 gcaccatcga aaccacattg atgggcagca ttatttccgc attcaagaca cggggcgaca
     7681 acggcgcgcg cgaaatcctg accgaatgga aaaacagccc cgtctcatcc gccgtttacg
     7741 tcatacaggg cgacgagaaa aaagacatct taaaccgcta tatcgacaat tacaccatag
     7801 aacgcgcccg gctgtttgcc gccaacaacc cccattccaa ccttgtccgc atcgaatacg
     7861 accgtttcgg cgaagaatac ctgttcttca ttaaaggctg ggacaaccac caggcacaac
     7921 gcctgcccag cccgctgttt atcccgggcc tgccgcttgc cccgatttgg cacgaattca
     7981 tcatcctctc cttcatcatc attgtcggac tgctgatggc atatatcctt gccggcaaca
     8041 ttgccaaacc catcagaatc ttaggcaacg gcatggacag ggtggcaaac ggagaacttg
     8101 aaacccgcat ctcccaacag gtcgacgacc gcgacgacga attgtcccat cttgccatcc
     8161 aattcgacaa aatggcggaa aaactcgaaa aacttgtcgc caaagaacgt tacctactcc
     8221 accacgtttc ccacgaaatg cgctccccgc ttgcccgtat gcaggcaatt gtcggactga
     8281 ttcaggcaca gccccaaaaa cgggagcaat atctcaaacg gctggaaggc gaactgaccc
     8341 gcatggatac gctggccggg gaactgttaa ccctgtcccg cctcgaaacc tccaatatgg
     8401 ctttggaaaa agaaagcctg aaactcctgc ccttcctggg caacctggta gaagacaacc
     8461 aaagtatcgc ccaaaagaac ggacagacgg tcgccctgtc tgccgacgga aaaatccccg
     8521 aaaacacaac catccttgcc aacgaaagct acctgtaccg cgccttcgac aacgtcatcc
     8581 gcaacgccgt caactacagc cccgaaggca gcaccatcct gatcaacatc ggacaagacc
     8641 acaaacactg gataatcgac gttaccgaca acggccccgg cgtggacgaa atgcagctcc
     8701 cgcacatctt caccgctttc taccgtgcag actccagcgc caacaaaccc ggaacaggac
     8761 tggggcttgc attgacccaa catattattg aacagcactg cggcaaaatc atcgccgaaa
     8821 acatcaaacc gaacggtctg cggatgcgct ttatcctgcc caagaaaaaa accggtttca
     8881 aaacagaaaa aagtgcgaac tgaccataat gccgtctgaa cccgcttcag acggcattgc
     8941 acaaacagtt accccaaaac aaacacacag cgggtgcatc tccaaaaatc acgcacatac
     9001 cgtccgcaaa ggaatatatc atgccggcac aaaccgatcc gggctacttc ttcatgccca
     9061 accacattat cctgataggc gcgagcgaac aaccgtacag cctgggtgaa cgcgtactca
     9121 gcaacctgct gagtacgccc tttcaaggaa aaatcacccc cgtaaacccg cgccaccaca
     9181 ccatagccgg gcttcccgcc tacaccagcc tcaacaaaat ccccggcagt gcagacctga
     9241 ttattgccgt taccccgccc gacagttacg acaccctcct caaaacctgc cgtaaaaagc
     9301 agctccgaca catcatcctc atacaggact gggacagcct gtctgccgca gaactgcaca
     9361 ccgccgaaac tgccatccgc aaacaccacg gcaacggact caacatcacc gcctgcacca
     9421 ccgcaggcat ccaactgccc tcactcggac tcaacatcag tacccaagac ggatatgccg
     9481 caggccatac cgccatactg accggcaatg ccgccgtcag ccgccaaatc gacaacatcc
     9541 tgaacaaact ccgtcaaggc acatcccgcc acatcagcct gcatcccggc atcagcccca
     9601 tcacatccgc cgattggctc aaccgcttcg gacacagcct gcacaccaaa accgccgtcc
     9661 tacaccacaa ccctgaagag gatcagcgca ggctgttcag cgcaatccgc caatttaccc
     9721 gccatacgcc gctgattctc cacatcacct gcctcacgac agaaaccgac cgtgccgtac
     9781 tgcactgcct cgcccgacac tgcaacttcc tcatcagttt caacgccgac gacctcgaag
     9841 ccgcactgag cgcccaactg tccgaccttc cacccctgtc ccgactcgac atcctgtccg
     9901 acacgcctgc cgaatggctg cacgcgcacg caccaaaaaa cctcaccctc cactttccca
     9961 accttcccca ccacatccgc aacggacacc tgaccggcac acccacaccc tcaatctgcc
    10021 acgacatcgc ctcacgtcag cttgcccgcc ccgacaccca agccgtcctg accatcctcg
    10081 gtccctccgg acacgaggat tacaaaaaaa cagcacgcgc ccttatccgc ctgtccgaac
    10141 agaccgccaa acccctgctc gtcagcagcc ccttttcaga cggcataaca catttcgaca
    10201 cccccgcaca ggcaatccgc accctttcct accgtaacac cgccgccgcc ctgaaacagg
    10261 cacagctcga cattgcaccg ccgcagccat gccgtctgaa aacaccgcaa ccccaaaaca
    10321 tcaaaaaagc ccttgcagcg gcaaatcccg ccctgctcgc cgaagccctg cacctccccc
    10381 taccggcaca ccacccataa cgccgtccaa ttccaattcg gcagccaccc cctctatggc
    10441 gacatcctga ccgcacgctg caacggacaa accactgccg tactcccgcc gtttaccacg
    10501 ctcgacagcc gccaccttgc ccgctttgcc gaactcgacg gcacacaaac cctcgaccag
    10561 ttcctgcaca cactgaccgt cattcccgaa taccgccaac acattttcgg catcaccctc
    10621 aacctcaacg gcggacaata cagcagcgat ttcatcttaa agacacccgg aacacacgac
    10681 acgcccaaac gcaagaacac aggcaaagcc gcccaaaccc tcgaacatgc cgccgcaaaa
    10741 atgcagagtg ccgccgcata cctggaacac aaaaacccga cagccgccga atttctccgc
    10801 cacacaagcg aagccgccgc agaactgctc ggcagcaaaa ccgaaaccgg agcagccgta
    10861 cccaacgtac ttgcccccta tcccgcagca caccccaaaa cactgtccct aaaaaacaac
    10921 acgaccgtta ccattatccc ccttttaccc gaagacgcag aagccaaaca gcagttcgtc
    10981 cgcagcctcg gccccgaagc acggtacaca cgcttcatga cccacaccaa cgaactgccc
    11041 gcagccacgt tggcacgcct gtgcaacccc gattaccact gtgaaccgca tggacggcaa
    11101 gggatgccga cagcaacatc gtcgccgtcg tccgccacag ccgcctgaac cgcaacgaat
    11161 gcgaatttgg catcacactg gcggaacata tgcgcggcag cgggctggca cagaaaatga
    11221 tggaactcat catccaaacc gccgcacagc aaggctacct gagcatgagt gccgacattc
    11281 tcaaaaccaa tacccccatg atcaaacttg ctgaaaaatc aggatttacc ctcaaggaat
    11341 cggacaccga aaaaaacctg taccgcgcat atctgaacct tgcggcagac aaaacaacag
    11401 gaaaaacaaa taaaaacttg cgcaccggcc acaaaataac ctaaaatcgg cagtttccat
    11461 atatccgatt ttctcaaaag gactcaaaat ggtagttatc cgtttggcac gcggcggctc
    11521 gaaacaccgc cccttctaca acgtcatcgt tactgactca cgcagccgcc gcgacggccg
    11581 cttcatcgaa cgcgtaggct tctacaaccc cgtagccaac gaaaaacaag agcgcgtccg
    11641 cctcaatgca gaccgcctga accactggat tgcacaaggc gcgcaagtca gcgactccgt
    11701 tgcaaaactg attaaagaac aaaaagccgt ctaatccgcg tttgccgcca tgacagacgc
    11761 tcaaaaccgg gtagccatgg gctacatcaa aggcgtattc ggcataaaag gctggctgaa
    11821 aattgccgcc aacaccgaat attccgacag ccttttggac taccccgagt ggcatttggc
    11881 caaggacggc aaaaccgtca gcgttaccct tgaagccgga aaagtcgtca acggcgaact
    11941 ccaagtcaaa ttcgaaggca tagacgaccg cgattcagca ttctcattgc gcggttacac
    12001 catcgaaata ccccgtgaag cattcgcccc gacagaagaa gacgaatact actgggcaga
    12061 cttggtcggc atgaccgtcg tcaacaaaga cgataccgtt ttaggcaagg taagcaacct
    12121 gatggaaacc ggcgcaaacg acgtattgat gattgacgga gaacacgggc agattctgat
    12181 tccgttcgtt tcccaatata tcgaaaccgt cgataccggc agcaagacca ttactgccga
    12241 ctggggtttg gactactgat gcttatccag gcagttacca ttttccccga aatgttcgac
    12301 agcattaccc gctacggcgt aacgggacgc gcgaacagac agggaatctg gcagtttgaa
    12361 gcagtcaatc cccgaaagtt tgccgacaac agattgggct atatcgacga ccgcccgttc
    12421 ggcggcggtc cgggaatgat tatgatggct ccgccgcttc atgcggcgat agaacacgcc
    12481 aaagcacaat cttcccaaac cgcaaaagtc atctacctca gcccccaagg aaaaccgctg
    12541 acacaccaaa aagcggcaaa actggcagaa cttacgcatc tgattctgct gtgcggacgc
    12601 tatgagggaa tagacgaaag actgctgcaa agcagcgtcg atgaagaaat cagcatcgga
    12661 gacttcgtcg tttccggcgg agagcttccc gccatgatgc tgatggatgc ggtattgagg
    12721 ctcgtacccg gcatattggg cgacattcag tctgccgaac aggattcgtt ctcaagcggt
    12781 attttggact gcccccacta caccaaaccc ttagaatttc aaggcatggc tgttccggaa
    12841 gtattgcgct ccggaaatca tggcttgata gcggaatggc ggttggaaca atcgctgcgc
    12901 cgcaccttgg agcgcagacc cgatcttttg gaaaagcgcg ttttaatccc aaaggaatcc
    12961 cgcctcttga ataaaatcct acaagagcaa cgggaaatcc aatcataatt taggaaaaaa
    13021 caatgaacct gattcaacag ctcgagcaag aagaaattgc ccgcctgaac aaagaaatcc
    13081 ccgaattcgc accgggcgac accgtagtcg tatccgtacg cgtcgtggaa ggtacccgca
    13141 gccgtctgca agcctacgaa ggcgtggtta tcgcccgtcg caaccgtggt ttgaacagca
    13201 acttcatcgt ccgcaaaatc tccagcggcg aaggtgttga acgtactttc caactgtatt
    13261 cccctactgt tgagaaaatc gaagtcaaac gccgtggcga cgtacgccgt gccaaactgt
    13321 actacctgcg cggtctgacc ggcaaagctg cacgcatcaa agaaaaactg cctgcacgca
    13381 aaggttgatt caaaccgttt tcccccaatg ccgtctgaac gttcagacgg cattttttat
    13441 tgccgcgatg ccggcaaggc aacgatatgc ctgaccctca tccaccttaa ttaacaatca
    13501 ctcccgccta ttcaagcaga atttacagac taaacgttat acagtatcat atccgccata
    13561 taacgataac cgctttgcca acttcccttt caaaactgca aatcattatg acaccttccc
    13621 tcttactgtc aggattgacc ttccgactct tcctcgccct gattgccgta ttccttttat
    13681 ggggtgttta cctttgggcg gtatcggtat gagcatcatc gttgaaaacc tgacggtcag
    13741 ctaccgccgc cgccccgccg tacaccacgt cgacattact tttgaagaac atagtatgtg
    13801 ggcggttttc ggtcccaacg gcgcaggaaa atctaccttt ctcaaatcct taatgggatt
    13861 gcagcctatc gatacgggca gcatccggct ggacggattg acccgtcaaa acattgccta
    13921 tctcccccaa caatccgaca tcgaccgttc ccagcctatg accgttttcg acttggcggc
    13981 aatggggctg tggtatgaaa tcggtttttt caaagggata aataccgctc aaaaacaacg
    14041 cgttcacgaa gccttggagc gtgtcggtat gcggcagttt gcccgccgcc aaatcgccca
    14101 cctctcaaac ggacaattcc aacgcgtcct tttcgcccgg atgctggttc aaaatgcaaa
    14161 attcctgctt ttggacgaac ctttcaatgc tgttgatgca cggacaacct acgagcttct
    14221 cgacgtgttg caaaaatgcc attgcggcgg acacgctatc attgccgttt tgcacgatta
    14281 cgaacaagtc cgtgcctact ttcccaatac cctgctgctc gcccgcgaaa aaattgcggc
    14341 aggcgcaacc gagaccattc tgacagaatc ctacctcgcc caagccagcg ccaaaatgca
    14401 gcaacaggaa agccccgact ggtgcgcctc ataaatactg cccgaaacca cgccccacac
    14461 cagacaccat gaacctctac gacctgctcc ttgccccctt tgcagaattc gacttcatgc
    14521 actacgccct cgcatccgtc ttctgcctgt ccctcagcgc cgcacccgtc ggcgtattcc
    14581 tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag ccacgccgtc ctgcccggtg
    14641 ccgccgtcgg ctacatgttt gccggcttga gcctgcccgc tatgggtgtg ggcgggtttg
    14701 ccgccggtat gctgatggcg ctgcttgccg gactcgtcag ccgctttacc accctgaaag
    14761 aagatgccaa ctttgccgcc ttttacctga gcagcctcgc catcggcgta atcctcatca
    14821 gcaaaaacgg cagcagcgtc gatttactcc acctcctttt cggatctgtg cttgccgtcg
    14881 atattcccgc actgcaactc atcgccgccg tctccggcct cacgctcatt acccttgccg
    14941 tcatctaccg ccccctggtg ctagaaagca tagaccccct gttcctcaag tccgtcaacg
    15001 gcaaaggcgg gctttggcac gtcattttcc tcatcctcgt cgttatgaac ctcgtatccg
    15061 gcttccaagc tctcggcatc ctgatgtcgg tcggacttat gatgctgccc gccattaccg
    15121 cccgcttatg ggcaagaaat atggggacgc tcattctgtt gtccgtcctc atcgcccttt
    15181 tttgcggttt gatcgggctg ctcatttcct accacatcga aatcccttcc ggccccgcca
    15241 tcatcctctg ttgcagcgtc ctttatctct tttccgtcat actcggcaaa gaaggcggca
    15301 tcttgcccaa atggttcaaa aaccaccgcc accacaccac ctgaccccgc aagccgcttg
    15361 aacaaacagg cggcttgttt taccataatg aatgttatgt tatatcacag aagaacagtt
    15421 atgaaacacc tcaaactcac ccttattacc gcattgctgg ccaccgccgc aactgccgca
    15481 ccccttccgg ttgtaaccag tttcagcatt ttaggcgacg tagccaaaca aatcggcggt
    15541 gagcgcgtag ccgtacaaag cctcgtcgga gccaaccaag atactcatgc ctatcacatg
    15601 accagtggcg acattaaaaa aatccgcagt gcaaaactcg tcctgctcaa cggcttggga
    15661 cttgaagccg ccgacatcca acgcgccgtc aaacagagca aagtatccta tgccgaagcg
    15721 accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca cgaccacgat
    15781 catgaccacg aaggacacca ccacgaccac ggcgaatatg acccccacgt ctggaacgac
    15841 cctgttctta tgtccgacta tgcccaaaac gtcgctgaag ccctgataaa ggccgatccc
    15901 gaaggcaaag tttattatca acaacgcttg ggcaactacc aaatgcagct taaaaaactg
    15961 cacagcgacg cacaagccgc atttaatgcc gtccctgccg ccaaacgcaa agtcctgacc
    16021 gggcacgacg cattttccta catgggcaac cgctacaaca tcagcttcat cgccccgcaa
    16081 ggcgtgagca gcgaagccga gccgtccgcc aaacaagtcg ccgccatcat ccggcaaatc
    16141 aaacgcgaag gcatcaaagc cgtatttacc gaaaatatca aagacacccg catggttgac
    16201 cgcatcgcca aagaaaccgg cgtcaacgtc agcggcaaac tgtattccga cgcactcggc
    16261 aacgcgcccg cagacaccta catcggcatg taccgccaca acgtcaaagc cttgaccaac
    16321 gcgatgaagc aataaaaccg tttgattggt ttgaatttga aaaggcaaaa ggccgtctga
    16381 aaattaaata cgaggttttc agacggcctt ctcttgtgca ataaagcagt aaacaaatat
    16441 tgataagaaa aaaacaatca gta
//
LOCUS       DAXFWM010000054        16364 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000054 DAXFWM010000000
VERSION     DAXFWM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.054"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(257..1042)
                     /locus_tag="ACFI8B_001586"
     CDS             complement(257..1042)
                     /locus_tag="ACFI8B_001586"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC3834765.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1286..2101
                     /locus_tag="ACFI8B_001587"
     CDS             1286..2101
                     /locus_tag="ACFI8B_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC3834766.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2142..3011
                     /locus_tag="ACFI8B_001588"
     CDS             2142..3011
                     /locus_tag="ACFI8B_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC3834767.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3047..4303
                     /locus_tag="ACFI8B_001589"
     CDS             3047..4303
                     /locus_tag="ACFI8B_001589"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC3834768.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(4361..4501)
                     /locus_tag="ACFI8B_001590"
     CDS             complement(4361..4501)
                     /locus_tag="ACFI8B_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834769.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            4649..5077
                     /locus_tag="ACFI8B_001591"
     CDS             4649..5077
                     /locus_tag="ACFI8B_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3834770.1"
                     /translation="MNFALSVITFTLASFLPVPPAGTAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5126..6469)
                     /locus_tag="ACFI8B_001592"
     CDS             complement(5126..6469)
                     /locus_tag="ACFI8B_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC3834771.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6474..7127)
                     /locus_tag="ACFI8B_001593"
     CDS             complement(6474..7127)
                     /locus_tag="ACFI8B_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC3834772.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7277..7567
                     /gene="gatC"
                     /locus_tag="ACFI8B_001594"
     CDS             7277..7567
                     /gene="gatC"
                     /locus_tag="ACFI8B_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC3834773.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            7630..9075
                     /gene="gatA"
                     /locus_tag="ACFI8B_001595"
     CDS             7630..9075
                     /gene="gatA"
                     /locus_tag="ACFI8B_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC3834774.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQVGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9072..10004
                     /locus_tag="ACFI8B_001596"
     CDS             9072..10004
                     /locus_tag="ACFI8B_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC3834775.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVTKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            10047..11477
                     /gene="gatB"
                     /locus_tag="ACFI8B_001597"
     CDS             10047..11477
                     /gene="gatB"
                     /locus_tag="ACFI8B_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC3834776.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11704..12714
                     /locus_tag="ACFI8B_001598"
     CDS             11704..12714
                     /locus_tag="ACFI8B_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC3834777.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPIRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(12844..13476)
                     /gene="pdxH"
                     /locus_tag="ACFI8B_001599"
     CDS             complement(12844..13476)
                     /gene="pdxH"
                     /locus_tag="ACFI8B_001599"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC3834778.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13536..13688
                     /locus_tag="ACFI8B_001600"
     CDS             13536..13688
                     /locus_tag="ACFI8B_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834779.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13697..14746
                     /locus_tag="ACFI8B_001601"
     CDS             13697..14746
                     /locus_tag="ACFI8B_001601"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC3834780.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14805..16346)
                     /locus_tag="ACFI8B_001602"
     CDS             complement(14805..16346)
                     /locus_tag="ACFI8B_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC3834781.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIVGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
BASE COUNT         4167 a         4983 c         4039 g         3175 t
ORIGIN      
        1 ttccccggtg tctgttttct gccacagccg atttagattc cagcaaaccg cctttaaaca
       61 attgaaagaa aagggacaaa ttgcacaggg ttctccgttt aaccagatac ggatttccat
      121 tcccggcaat ttcgggcatt tgaccgtttc cgccttcctg ccccgccgcc aataaaaatg
      181 ccgtctgaaa tatcggcacg atacttcaga cggcataccc gcccgttttc ccgcatttcc
      241 gggagctggc tgaaatttaa acgtgtgcgg aaatgatttt taacatttgc gccagtactt
      301 tgggattcgc agccacaata tcgccgcttt ccaaccaagc gtcctcaccc gacatatcgg
      361 taacgatgcc gcctgcttct tggacaatca atgcaccggc ggcaatgtcc cacggtttga
      421 ggttgaactc gaaaaagccg tcaaaacgtc ctgttgctac ggcgcacaaa tccaaagaag
      481 ccgcgccttc gcggcggcct ccggcggttt ttgccaaaaa gtctttcaaa atcgccagat
      541 atttgtccat catgctttga tcgacaacag ggaaaccggt accgatcagg cagcggttca
      601 attcgatgcg gttggagaca cggatgcggc ggtcgttaag caacgcgcct ttaccgcgcg
      661 aagccatgta cacgtcgtta cgttcggggg cgtacaccaa agcttcctgc aacacgcctt
      721 tgtgcagcag tgccatagag atggcgtatt ggggatggcc gtgaaggaaa ttggtcgtcc
      781 cgtcgagcgg atcgataatc cattcgtact cggctgtagc tttgccgtgg gagccgcttt
      841 cttcgcaagt gattttgtga tgcggatagg cttctttcaa agcctcaacc aggatgattt
      901 cggaattgcg gtcaacatcg gaaacaaaat cgttgaaggc tttgctgtcg gttttgacgg
      961 catcgaggtt acctgcggcg cgaatcatca tctgaccggc acggcgggcg gctttaaagg
     1021 ctgtattcaa aaacggattc atcagatttc cttaagggtg gcataccgcc ggttcggacg
     1081 tgcagtcctt cggagcggca aaatcggagt ttatctggtt ggggcaaacc ctgccaaatc
     1141 gggtaaaata ccgcctgacg cgtgtctgct tcaggcgcaa cgttaaattt ccgacgttgt
     1201 taaagaacag ttcaaacggc atttcaccgt ccgaacgaaa aagacggcgc attataccct
     1261 attccattcc gaccgaaaac cgaacatgac tgctctcaaa cccgccctgc ccgactacct
     1321 cggcaacatc cgcatcatcc tcacacgcac cagccacccc gccaacatcg gctctgccgc
     1381 acgcgcgatg aaaacgatgg gtctgcacag gctgaccatc gtcaccccca atctgatggc
     1441 aacgccgatg acggaaaatc cgcccgtttt taatcccgat gacgtgcaaa gttttgcact
     1501 gcctgaagaa agtttcatcc tcgcttccgg cgcggcggac gtattgcaca acgccgaaat
     1561 cgtcgccaca ctggacgaag cccttgccga caccaccatc gcctgcgccc tgaccagccg
     1621 ccgccgcgaa atcaccgcgc cgctgcaaac cccgcgcgat ttggtgcccg aattactgca
     1681 ggccgccaac cgcggcgaga aagtggcgct ggtcttcggc aacgagactt tcggcttgag
     1741 catcgaagaa gtccgagcgt gcaaccgact gatgaccatc aacggcaacc ccgactattt
     1801 ctcgctcaac ctcgcccaag ccgtgcaggt cgtgtgctac gaaattttca gccaaaccga
     1861 ttcgcccatg acccatcttc aacaggaaga ccacgccgca acccacgagc aaatcaaagg
     1921 catgctcgcc cacatggaaa gcgtgatgga cgacatcggc tttttcaacc gccgcaacgg
     1981 cgagcgtctg atgcgccgta tgcagagcct gttcggacgc gccaacacgc aaaccgaaga
     2041 catcgacatc ctgcgcggtt ttttcaatac cgtcagccac cgtatccata aaaaagactg
     2101 attaaggccg tctgaaaaca ttacaagctt tttcagacgg catgactgat attcggataa
     2161 gcatgaatta cgccctagac gcattatggt ggaaactcac cagccaaccc gtccgcgacc
     2221 tcgcctcgct gctgactgcg ccgcctttgt ggcaaagcgg ttgcgaattg agcgtgcgcg
     2281 aactactggg ggaacgcggt ttccgctacc ttttagcatt ggatgccgat cccgcgccgc
     2341 tgacggatta ccttgcacaa cgcgccccgt tcgaccaccg tctcggcatt tatgccgaag
     2401 agctgctggc tttttggttt accaacgcac cgcacaccaa actgcacgca tataatctga
     2461 ccgttttttc agacggccaa actttaggcg ccgcggattt tgtcgtttcc ctcaaccaac
     2521 agccctatca tatcgagctg gcgtgtaaat actacggcgg agaccaagtg cagaacctgc
     2581 gcggtctcaa tcccaaagac acgctgacgg acaaagccgc caaattggtg cagcaattcc
     2641 agctggtgca tacgtcgcaa ggcaaagcaa ccttagccgc acaagacctg ccggaaaatc
     2701 cacttcctgc ttccatcgtg cgcggcatcg gattttttcc acaaggtttc catgcttttg
     2761 agccgccgct taatccatac ggctggcgcg gcatctatat tcaagattgg gcggaatacg
     2821 ggtttgaacg ccaagaagcg cgctatcacc tgctcgaccg catggtctat ctcgcgcctg
     2881 cgcgtgtcgc cgaaaccgaa acattaaacg caaccgaaat ccgccgcatc gaccaaggct
     2941 tgattgccgt tttggaatgt cggccggacg gcttttggca cgaaatcgaa cgtattatga
     3001 aggccgtctg aaacccttta ccaactttaa gagaacccat tacattatga acgccgcaca
     3061 actcgaccat accgccaaag ttttggctga aatgctgact ttcaaacagc ctgccgatgc
     3121 cgtcctctcc gcctatttcc gcaaacacaa aaagctcggc cgccaagacc gccacgaaat
     3181 cgccgaaacc gcctttgccg cgttgcgaca ctatcaaaaa atcagtaccg ccctgcgccg
     3241 tccgcacgcg cagccgcgca aagccgcact cgccgcgctg gttctcggca gaagtaccaa
     3301 catcagccaa atcaaagacc tgcttgatga agaagaaaca gagtttttcg gcaatttgaa
     3361 agcccgtaaa accgagtttt cagacggcct gaataccgcc gcagaattgc cgcaatggct
     3421 ggtggaacaa ctgcaacagc attggagcga agaagaaatc ctcgctttcg gccgcagcat
     3481 caaccaagct gcgccgctcg acatccgcgt caataccttg aaaggcaaac gcgacaaagt
     3541 attgccattg ttgcaagccg aaagccccga tgcagaggca acgccttatt ctccttgggg
     3601 catccgcctg aaaaacaaaa tcgcgcttaa caaacacgaa ctgtttttgg acggcacgct
     3661 ggaagtccaa ggcgaaggca gccagctgct tgccttattg gtgggcgcaa aacgcggcga
     3721 aatcattgtg gatttctgtg ccggagccgg cggtaaaacc ttggctgtcg gcgcccaaat
     3781 ggccaacaaa ggcagaatct acgccttcga catcgccgaa aaacgccttg ccaacctcaa
     3841 accgcgcatg acccgtgccg gactgaccaa catccacccc gaacgtatcg gcagcgaaca
     3901 cgatacccgt atcgcacgac tggcaggcaa agccgaccgc gtgttggtcg atgcgccctg
     3961 ctccggctta ggcacactgc gccgcaatcc cgacctcaaa taccgccaat ccgccgagac
     4021 cgtcgccaaa cttttggaac agcaacacag tatcctcgat gccgcctcca aactggtgaa
     4081 accgcaaggc tgtttggttt acgccacctg cagcgtgttg ccggaagaaa acgaactgca
     4141 aatcaaacgt ttcctgtccg aacatcccga atttgaaccc gtcaactgcg ccgaactgct
     4201 gcaaaacctg aaggtcgatt tggataccgg caaatacctg cgcctcgatt ccgcccgaca
     4261 ccaaaccgac ggcttcttcg ccgccgtatt gcaacgcaaa taaaccggtt tgaacaaaat
     4321 gccgtcagaa cccttttcaa agcgttcgga cggcatttca tcaattaaaa atatcaaaaa
     4381 ccgcagattc aaccgattca acaccaaagg cagaatcacg cgcctcatcg gattttatac
     4441 gcgcctgcct gcaattttct ttttctttgc caaaatatac ggtagcataa ccatattcca
     4501 atgatatttt tgggaatctg ttttacccca atatataaag caccatatta aggcggagtg
     4561 tcttccctgc cttgacccga acccggaaaa gacaccgccc gaaccaatcc cgattctgcc
     4621 cgggcagtca gccatcaagg aaaccctaat gaactttgct ttatccgtca tcacatttac
     4681 cctcgcctct ttcctgcccg tcccgcctgc cggaaccgcc gtctttactt ggaaagacgg
     4741 cggcggcaac agctattcgg atgtgccgaa acagcttcat cccgaccaga gccaaatcct
     4801 caacctgcgg acgctccaaa ccaaaccggc ggtcaagccc aaacctgccg tcgatacgaa
     4861 tgcggacagt gcgaaggaaa acgaaaagga tatcgccgag aaaaacgggc agcttgagga
     4921 agaaaagaaa aaaattgccg aaaccgaacg gcagaacaaa gaagaaaact gccggatttc
     4981 aaaaatgaac ctgaaggcgg tgggaaactc aaatgcgaaa aacaaggatg atttgatccg
     5041 taaatacaat aacgccgtaa acaaatactg ccgttaatcg gctctagcgc aaacccgatg
     5101 ccgcctgaag cggcacgggg tttgtttatt tctgccagta ggttttgacg ttgacgaact
     5161 cgtacaaccc gaattcggac agttcgcgcc cgtaaccgga atctttgact ccgccgaaag
     5221 gcaggcgcaa atcgctgctg gtatggcggt tgataaacac cgatccggcc tgtatttttt
     5281 cggcaaaccg ccaggcgcgt tcagtatcgg cggtataaat gcaggcaccg agcccgaacg
     5341 gggaatcatt ggcaaggcag atggcgtgtt cttcgttttc ggcgcgcaaa atcagggcga
     5401 cggggccgaa tacttcttct ctccagacgc ggcaggcagg atttaccctg tctaaaaccg
     5461 tcgcagggta aaaccacccc gacccctgtg gaactttgcc tccggtcagg cacaccgcgc
     5521 cgtttgaaac ggcatcttca acctgcccgt gaacccggtc ccgcaaatct tcgcggtgca
     5581 gcggtgcaag cgtggtatcg ggatgtttcg ggtctcccat tttcaatttg gcgcattcgg
     5641 cgagaaacag ctgaataaag cggtcggctg cggcttcggt tacgatgatg cgcttggcgg
     5701 cgttacacga ttgccccgca tcgcggaaac gggaataaca ggcttctgcg gcggcgcgtt
     5761 ccaaatcggc atcgggcatc acgataaagg cgttgctgcc gccgagctcc aacacggttt
     5821 tcttgaggtt tgcgcccgca tgtgccgcaa gaatgcgccc cgtatgcgtc gaaccggtaa
     5881 acgccatcgc atcggtatct tcgactgcct tgagcgtgtc cgcctcatcc agccacgcgc
     5941 ctgccagagg aataccgtct gaagccaaat cgaacaacgc ccgactgacg cgtgccacgc
     6001 tgggcgcggg tttgacggcg cacgcgttgc ccgcgcacat agcgggaacg gcgaaacgca
     6061 atacctgcca gacgggatag ttccaaggca tgacggcaaa caccacgccc aaaggctcga
     6121 agcgcacctg actcaaactc gcctgcgtcg caatggtttt gtgggcaagc agttcggggg
     6181 caaggcgggc gtaatagcgt atcagttcga tagacttgac gatttcggca cggcattcgt
     6241 gcaggcagcg tccgacttct tcgcacacca tttccgcaaa acgctctttt tccgcctcca
     6301 aacggccggc aaatttttgc agacgcgcgg cacgttcggt tacgcccaat tgcgcgaacg
     6361 ccctgccgtg cattttcaaa tccgccagcc gccgttcaaa ctccgcataa tcttgatcgg
     6421 ggcggcggta aagcgtttcg cccgtaaata cattgacact gtgaaacatc gaatcaacct
     6481 gccagttgcg ggaatatcgt tttcagtccc gacacaataa tctccaccga taccgccgcc
     6541 agcatcatac ccataatgcg gtttaaaatc gtcagccccg tcgcgcccag caggcggctg
     6601 accttcccgg caacgattaa aatggcataa caaatcgcac tgaccaccaa accggccgcg
     6661 ataatcagcg cgatatcgct gtacgttttg gctgccgaag cataaataat cacagtcgaa
     6721 ataccgcccg gaccgatggt gatcggtatg gcgatgggca cgacggcaat cgcccctgca
     6781 ttgcgggcgg ggcgcgcttg ccccgtttcc ggctgcgcgc cgagattctg cttggcggga
     6841 ttgtcgttgc cgttcatcat cgaaatggcg atcagcagca ccaaaatccc gccgccgacc
     6901 tgaaacgaac cgacgctgat gcccaaaacc ttcaatagcg caccgccgat cagcgcaaat
     6961 accgcaatca cggcaaacac ggcgacggcg gccgtccgcg cgaccttcct gcgctccttc
     7021 gtgctgtgtc cgttggtcag gtcaaggtaa agcgacaacg cgctaaacgg attgatcagc
     7081 accaaaaaag ccacaatcag cttgccgatt tccataccca atcccattat ttcccctctt
     7141 tcaaacccgt gcggcaggca tccgatgctg caaattgccg ccgcaacgga tttttccgtt
     7201 ataattaaaa atccaagcaa tacgccccat catacccgaa cgacggtatc tttaccatca
     7261 gacaaggatg cttttcatgg cactgacact tgccgacgta gacaaaatcg cccgactctc
     7321 ccgactgcag ctgactgcgg aagaaaaaga aaaatcgctt caagaattaa acgacatttt
     7381 cactatggtc gaacagatgc agaacatcaa cacagacggc atcgagccga tggcgcaccc
     7441 gcacgaggtc gccctccgcc tgcgtgaaga cgaagtaacc gaaaccgacc gcgccgccga
     7501 ataccaagcc gttgcgccgg aagtacgcaa ccgcctgtac atcgtaccgc aagttatcga
     7561 agaataatcc gaatacgctt cagacggcat cagcaatacc gcccgaagcc ctttaaggat
     7621 ggaagattta tgacccaata cacattgaaa caggcaggca gcctgttgca gtccaaacag
     7681 atttccgccg tcgaactggc aagcgcatac cttgccgcca ttgccgaaaa aaatcccgcc
     7741 ctcaacggct acatcaccat cgaccaagat aaaaccctcg ccgaagcccg tgccgccgac
     7801 gaacgcatcg cacagggcaa cgcttcggca cttaccggcg ttcccgtcgc ctacaaagac
     7861 attttctgcc aaaccggctg gcgcagcgcg tgcgcgtcca aaatgctcga caacttcgtc
     7921 tccccctaca cggccaccgt cgtccaaaac ctgctcgacg aaggtatggt aacgctcggc
     7981 cgcaccaaca tggacgagtt cgctatgggt tcgaccaatg aaaactcatt ctacggcgcg
     8041 gccaaaaacc cgtggaatcc ggaacacgta cccggcggtt cgtcaggcgg ttccgccgcc
     8101 gtcgttgccg cgcgcctcgc ccctgccgcg ctcggttcgg acacgggcgg ctctatccgc
     8161 caacccgcat cgcactgcgg cattaccggc atcaaaccta cctacggcac ggtttcccgc
     8221 ttcggtatgg tcgcctacgc ctccagcttc gatcaagtcg gcccgatggc gcaaaccgcc
     8281 gaagactgcg cgattctgtt gaatgcaatg gcaggtttcg accccaaaga ctccaccagc
     8341 ttcgaacgcg aaaaagaaga ctacacccgc gatttggaca aaccgctcaa aggcgtgaaa
     8401 atcggcctgc ccaaagaata cttcagcgaa ggcaacagca ccgatgttca gacggcattg
     8461 caaaacacca ttgatttgct gaaagcacaa ggcgcggaac cggtcgaagt ttccctgccg
     8521 caaaccaagc tgtccatccc cgcctactac gtcctcgcct ccgcagaagc cggcaccaac
     8581 ctttcacgtt acgacggcgt acgttacgga caccgtgccg cccaattcgg cgatttggaa
     8641 gaaatgtacg gcaaaacccg cgccgaaggt tttggcagcg aagtcaaacg ccgcatcatg
     8701 atcggcactt acgtactgtc gcacggctac tacgatgcct attatctcaa agcccaaaaa
     8761 ctgcgccgcc tcgttgccga tgactttcag acggcatttg cacggtgcga cctcatcctc
     8821 gcgccgaccg caccctccgc cgcacccaaa atcggagcgg atacttctcc ggttgaaacc
     8881 tacttgagcg acatttacac catcgccgtc aacctcgccg gactgcccgc attgaccctg
     8941 cccgcaggct tcagcggcgg cggactgccc gtcggtgtac agcttgtcgg caactacttc
     9001 gccgaagcca aaatcctcgg tgcggcgcat caaatccaac tcaacagcga ttggcacggc
     9061 aaacgacccg aatgaagcag aaccgcacct ttaccttccc cgattttcgc accgtttaca
     9121 gctatgcgcc tttatatcgg ctgcaacatt taaaatacac attgcgaaaa tttttcggaa
     9181 aaaaagaaat ttacgccttc gagcagtttg tcaacgcctc ccctatccgt caggggctgt
     9241 tcctccactg cccgcaggat gcctatccgc tgctgcgcga atttgttgac aggcgtttta
     9301 actgcaaacg ccgtttagat gcgatgacgg cagattttct catggcggaa aaactcttcg
     9361 gcacagacgt tctgtgccaa atggaagact gccgcttcca tctggtcttg gcgcaccttt
     9421 cagacggcat cagcttgtgg ctcaaccgca acgacaactg cgtcgaagaa ggcgcgtggt
     9481 ctttatcttt gcgcgatgaa gcaggcaacc ggctgtatat ggcgaccttc gcctttgtcg
     9541 gcacacacct gctgacagcc tccgtacaag ggccgtcagg tgaagaagcc aaagacaccg
     9601 tccgccgcat aaccaaacaa cttcacggct tacgtcccca acaactgatg gtaaccgcac
     9661 tgcaatattt cgccgccgca ttaaaattag acggcgcgat aggcattgca caaaaacatc
     9721 aagtcaaatt acgctggaaa cttaaaaagc gcgtcaaaat gaattacgac gcattctggc
     9781 aggaatacgg cgcaagtttg gaacgggacg gctactggca tcttccccaa acccccgccc
     9841 gcaaagacct tgccgacatc gaaagcaaaa agcgttcgat gtaccgcaag cgttatgaaa
     9901 tgctggacga tatggttaca aagatgaagg acagtctgaa aacagaagca cgcggcattt
     9961 cagacggcat ccaaacggaa aaaccgctcc gccggacagt ctgatgcgaa gactatcgaa
    10021 ttgatatttt agagaaagaa cctcctatga cttgggaaac tgtaatcggc ttggaaatcc
    10081 acgtccaatt gaacaccaaa tccaaaatct tcagcggtgc atcgaccgca ttcggcgcag
    10141 aacccaacgc gcacgccagc gtagtggaat gcgcgctgcc gggcgtactg ccggtaatga
    10201 accgcgaagt cgttgaaaaa gccatcaaat taggtttggc tttggatgca aaaatcaacc
    10261 ggaaaaacgt gttcgaccgc aaaaactact tctatcccga cttgccgaaa ggctatcaaa
    10321 tcagccagtt ggatttgccg attgtcgaac acggcaaatt ggaaatcgtg gtcggcggcg
    10381 atgtgaaaac catcaacgta acccgcgccc atatggaaga agacgcaggc aaatccgtgc
    10441 acgagggctt gaacggcgcg accggcatcg acctgaaccg cgccggcacg ccgctgttgg
    10501 aagtggtatc cgaacccgaa atgcgctccg ccgccgaagc cgttgcctac gccaaagcct
    10561 tacacagctt ggtaacctgg ctggacattt gcgacggcaa tatggcggaa ggttcgttcc
    10621 gcatcgatgc caacgtatcc gttcgcccga aaggccaagc ggaattcggc acgcgccgcg
    10681 aaattaaaaa cctcaattcc ttccgtttct tggatcaggc gattaattac gaagccgaag
    10741 cgcaaatcga gattttggaa gacggcggca cagttcagca ggcgacgatg ctgttcgacc
    10801 cggaaaaagg cgaaacccgc gtgatgcgcc tgaaagaaga cgcgcacgac tacggctact
    10861 tccccgatcc cgatttgctg cccgtcatca tttcagatgc ccaaatgcaa aaagccaaag
    10921 cagaaatgcc cgagctgccg aaagaaatgg cggcgcgttt cgtggcggat tacggcgtgt
    10981 ccgaatacga cgcgcgcctg ctcaccgcca gccgcgtgca ggctgcctat tttgaagaag
    11041 ccgccaaaga gagcggacaa ggcaagccga ctgccaactg gatgaacggc gaacttgccg
    11101 ctacgctgaa caaagaaggc atggaacttg ccgacagccc gattaccgcc ccacgccttg
    11161 ccgcgctggt gggcaaaatt gccgatggca cattaagcgg caaactggcg aaaaaagcct
    11221 ttgaagccat gtgggcggaa ccggaaacca gcattgccga aatcatcgaa aaacacagtt
    11281 tgcaacagat gaccgatacc ggcgcggttg aagcgatggt ggacgaagtg ctggcaaaca
    11341 acgccaaagc cgtggaacag tttaaatccg gcaacgaaaa agccctgaat gcgattgtgg
    11401 ggcaagtgat gaagaccagc aaaggcaaag ccaatcccgc tcaggtgcag gagttgatta
    11461 aagccaaact tgcctgataa gcaagatgcc gtctgaaacc caaatccgac gttcagacgg
    11521 cattttcaca gtctgaccac attaaaacag cataaaaaac aatattccac gtttcaacac
    11581 acaggacgac acataaaatg tcgccctgtg tgttgccctg atttgaaagg ggttacgccc
    11641 ctcccaaata aagtctgatc ctatcggcct aaagggtggg gtttcaaccg aaaaggaaat
    11701 acgatgaacc acaccgttac cctgcccgac caaaccacct ttgccgccaa cgacggcgaa
    11761 accgttttgt ccgctgccgc ccgtcaaaac ctcaatctgc cccattcctg caaaaacggt
    11821 gtctgcggac aatgcaaagc cgaactggcc agcggcgata ttcaaatggg cggacactcg
    11881 gaacaggctt tatccgaagc agaaaaagcg caaggcaaga ttttgatgtg ccgcaccacc
    11941 gcgcaaagcg atatcaacat caacatcccc ggctgcaaag ccgatgccct acccatccgc
    12001 accttgcccg cacgcatcga aagtatggtt ttcaaacacg atgtcgccct cctgaaactt
    12061 gccctgccca aagccccgcc gtttgccttc tacgccgggc aatacattga tttactgctg
    12121 ccgggcaacg tcagccgcag ctactccatc gccaattcgc ccgaccaaga aggcattttg
    12181 gaactacaca tccgcaggcg cgaaaacggt gtctgctcgg aaatgatttt cggcagcgaa
    12241 cccaaagtca aagaaaaagg catcgtccgc gttaaaggcc cgctcggttc gtttaccttg
    12301 caggaagaca gcggcaaacc cgtcatcctg ccggcaaccg acacaggcta cgcccccatc
    12361 cgcagcatcc tgctcgacct tatccgccaa aacagcagcc gcgctgccca tttctactgg
    12421 ggcgcgcgtc atcaagatga tttgtatgcc ctcgaagaag cacaagggtt ggcatgccgt
    12481 ctgaaaaaca cctgcttcac ccccgtattg tcccgtcccg gagagggctg gcagggaaga
    12541 aaaggccacg tacaagacat cgcggcacaa gaccaccccg acctgtcgga atacgaagtg
    12601 tttgcctgcg gctctccggc tatgaccgaa caagcaaaaa atctgtttgt gcaacagcat
    12661 aagctgccgg aaaacttgtt tttctccgac gcattcacgc cgtccgcatc ataattcctc
    12721 ggtataaaga ggattcgggc tttccgttcg gaacacaaaa aacttcccgt ccgtattttc
    12781 cccgtgaaaa aatgccgtct gaaacctgat tccggttttc agacggcata tgttttttcc
    12841 tgttcaaggc gacaaccgct cgcgtatcca gccgccatcc agcaaacggt attggatgcg
    12901 gtcgtgcagc ctgctcggcc tgccctgcca aaactcaatc aaatcgggaa tcacaatata
    12961 gccgccccaa tgcggcggac gcggcacgtg caaaggatgt ttgagtccga ccgccgccgc
    13021 ctttgccacc aataccgcct tgttcggaat cacctcgctc tgcgcgcttg cccacgcacc
    13081 caaacggctc tgatacgggc gactctcaaa atattcgtcc gacaacttct ccgccagcct
    13141 tccgacacgc ccttccacgc gcacctgacg ctccagctcc ggccaaaaaa acgtcatcgc
    13201 cgcaaacgga ttgagttcca gcgaacgccc cttgcggctg tggtaattcg taaagaaaac
    13261 aaaaccttct gaattaacct ccttcaaaag caccatacgg ctgttgggcc tcccgcgccc
    13321 gtcgaccgcc gccacattga ccgccgtcgg ctcgttgacc tcggcgcgta ccgcctcgtc
    13381 caaccaccgc tcgaactgct cgatcggatt atcggcgcaa tcggcttccg acaattcccg
    13441 tttgctgtaa tcttcccgaa tattgtgcaa atccatttac cgctcctctt atcatttgaa
    13501 agattctact cccgcacgca aaccgatttc aaccgttgca caaactttgt ccccaaagcc
    13561 gcagcgacga tttcatccgc aaaaccgccg catcaggtac aatatcgaac cgtccgaccg
    13621 aggacggcat tttatcaacc cgtcctgccg cacacgccgc agaagaaccg ccttatcagg
    13681 cgagttagga aaaatgatgt ccaagcaacc caccagcaaa cgccaatggc gcgacggcgc
    13741 agccccgtct gccaagaaaa ccgccaaacc tttcaaaagc aaagcccgtc ccaaagacga
    13801 aacgcgcaaa accgccgccc aagcttacgg acaaaaagct tcagacggca tcaagctgca
    13861 aaacgccccc aaacagcgcg ccgccaaagc caaaaaactc gtcgtccgca atcccaacca
    13921 aaaaattatg gaacacgcgc gcgatttgaa agaacgccgc agcgacctgt cgcgtatgga
    13981 acccgaacgc ctgcaaaaag tgcttgcagc ctccggcgtc ggctcgcgcc gcgaaatgga
    14041 agaatggatc aacaacggct gggtaacggt caacggcaaa accgcgcaac tgggcgacaa
    14101 agttaccccc gacgaccacg ttaccgtcaa aggcagcatc atcaaactca aatgggccga
    14161 ccgcctgccg cgcatcatcc tgtattacaa acaagaaggc gaaatcgttt cccgcgacga
    14221 cccgcaaggc cgcgtcagca tattcgaccg cctgccgcag gccgccagca gccgctgggt
    14281 cgccatcgga cgcttggaca tcaacaccag cggacttctg attctcacta cctccggcga
    14341 actcgtccaa cgtttcgccc accccagctt cgaagtcgaa cgcgaatacg ccgtgcgcgt
    14401 actgggcggg ctgactaccg aacaaatgcg cagtctcacc gaagaaggcg tgatgctcga
    14461 agacggcttg gcaaaagtcg aacgcatcta cgaacaaggc ggcgaaggcg caaacaaatg
    14521 gtacaacatc gtgattaaag aaggccgcaa ccgcgaagtg cgccgcatat tcgaaagcca
    14581 aggcctcaca gtcagccgcc tcgtgcgcgt cgccttcggc cccatcggac tgcccaaccg
    14641 cctcaaacgc ggacagttct acgaactcaa ccccgccgaa gtcgccaaca tcctcaaatg
    14701 ggcggatatg ctgctgccgg gcgaacgccg ccgcaaaaaa gcctgaaagt aaaaatgccg
    14761 tctgaaacac ctgctgtttc agacggcatt ttattcgggc gttttcagga gaaaaggtcg
    14821 agtgctttga caaagaccat caccacgccg taggcgagcg gtatgacggc caatgcccaa
    14881 cgccaccaga ccgatatgcc gtcgccggaa actttttcgc ccacaaggta agcgtcggat
    14941 atggcggttt cgtcgtcggg gttgccgctg cgtgcggcgg ttttgatgtc tttttcgtgg
    15001 tgtttttcgt gtacggattt gacggcgagg ttgcacaaca gtccgacaat caacaatccc
    15061 gccataatgt acatggttac gctgtatgcc tgtgccgccg gtatgccgct gtcgatttgg
    15121 ctttggcgta tgtaattgac cagtaccggg ccgatgacgg cggcggttga ccaggccagc
    15181 aggatgcgcc cgtgaatcgc gccgacctga taggtgccga acaggtcttt caggtaggcg
    15241 ggaatggcgg caaatccgcc gccgtacatg gaaataatca cgcagaagcc gatgatgaac
    15301 agggctttgc tgccgccttc gccgatggag ggaacggcga aatacagcag cgaaccgagt
    15361 acgaagaaga tggtgtaggt gtttttgcgt ccgattttgt cggaaacgct cgaccacaaa
    15421 aagcgtccgc ccatattaaa caggctcagg aggctgacga agcctgccgc cgcacctgcg
    15481 ccgactgccg cctgcctgcc tgcggaggtt tcggaaaaga gttcctgaat catcacggat
    15541 gcctggccca atacgccgat gccggcggtt acgttcaggc acaataccca gaacaacagc
    15601 caaaactgcg gcgttttcat ggcttgggac acgttgacat gattgctgct gaccggcttg
    15661 ttttgcgttt tcggcacggt atagccttca ggtttccagc cgtcggcagg tacgcggatg
    15721 gtaaacgcgc cgaacatcat cagtgcgagg taaaacaggc ccaatacggc aaaggtttcg
    15781 gcaaccccga ccgaagcggc gtttgaaaag gcgttcatca gtgatacgga aagcggcgag
    15841 gccagcattg cgccgccgcc gaaacccata atcgccaaac cggtcgccat acccggcttg
    15901 tcgggaaacc atttcatcag cgtggaaacc ggcccgatgt agcctaagcc taagcctacg
    15961 ccgccgatga cgccgttgcc caaataaagc aggaagaggt tgtgcgtacg cacgccgaat
    16021 gcggatacga agaagcccag gctgaaacag caggcggcgg caaatatggc tttgcgcggc
    16081 cctacccgtt ccatccatgt gccgaacagg gcggccgacg cgcccagcat cgcgagtgcg
    16141 atactgaaaa tccaacctac ggtcgtcagc ttccaatctc cggccgccga ttcggttatg
    16201 ccgataagtt tggtcagcgg cgcgttgaat acggaatagg cgtaaatctg cccgatggca
    16261 aggtgcaccg ctaatgctgc gggcggcacg agccaacggt tgaaacccgg cttggcaatg
    16321 cttgcctcac ggtctaaaaa cttcataaca tcctctttct gtca
//
LOCUS       DAXFWM010000055        16274 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000055 DAXFWM010000000
VERSION     DAXFWM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.055"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1516
                     /gene="yidC"
                     /locus_tag="ACFI8B_001603"
     CDS             <1..1516
                     /gene="yidC"
                     /locus_tag="ACFI8B_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC3834782.1"
                     /translation="ATASAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKAT
                     GDENKPFVLFGDGKEYTYVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSA
                     PETNGLKIDKVYTFTKDSYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTH
                     SYVGPVVYTPEGNFQKVSFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQ
                     PKGGQSVCAAGDCRIDIKRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVI
                     ANIADNLQLAKDYGKVHWFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNA
                     SYRSMAKMRAAAPKLQTIKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVF
                     IGLYWALFASVELRQAPWLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQA
                     KMMKIMPLVFSVMFFFFPAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(1597..2307)
                     /locus_tag="ACFI8B_001604"
     CDS             complement(1597..2307)
                     /locus_tag="ACFI8B_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC3834783.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(2369..2959)
                     /locus_tag="ACFI8B_001605"
     CDS             complement(2369..2959)
                     /locus_tag="ACFI8B_001605"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC3834784.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            3107..3607
                     /locus_tag="ACFI8B_001606"
     CDS             3107..3607
                     /locus_tag="ACFI8B_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC3834785.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            3641..3820
                     /gene="rpmF"
                     /locus_tag="ACFI8B_001607"
     CDS             3641..3820
                     /gene="rpmF"
                     /locus_tag="ACFI8B_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC3834786.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            3995..4522
                     /locus_tag="ACFI8B_001608"
     CDS             3995..4522
                     /locus_tag="ACFI8B_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC3834787.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDSEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            4612..5658
                     /gene="plsX"
                     /locus_tag="ACFI8B_001609"
     CDS             4612..5658
                     /gene="plsX"
                     /locus_tag="ACFI8B_001609"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC3834788.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            5796..6047
                     /locus_tag="ACFI8B_001610"
     CDS             5796..6047
                     /locus_tag="ACFI8B_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC3834789.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            6074..6292
                     /locus_tag="ACFI8B_001611"
                     /pseudo
     CDS             6074..6292
                     /locus_tag="ACFI8B_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6443..6634
                     /locus_tag="ACFI8B_001612"
                     /pseudo
     CDS             6443..6634
                     /locus_tag="ACFI8B_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6664..6867
                     /locus_tag="ACFI8B_001613"
     CDS             6664..6867
                     /locus_tag="ACFI8B_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834790.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            6894..7856
                     /locus_tag="ACFI8B_001614"
     CDS             6894..7856
                     /locus_tag="ACFI8B_001614"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC3834791.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            7903..8274
                     /locus_tag="ACFI8B_001615"
     CDS             7903..8274
                     /locus_tag="ACFI8B_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC3834792.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            8300..9226
                     /gene="fabD"
                     /locus_tag="ACFI8B_001616"
     CDS             8300..9226
                     /gene="fabD"
                     /locus_tag="ACFI8B_001616"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC3834793.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            9365..11233
                     /gene="msbA"
                     /locus_tag="ACFI8B_001617"
     CDS             9365..11233
                     /gene="msbA"
                     /locus_tag="ACFI8B_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC3834794.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPDLSAAAGILSTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            11333..12898
                     /gene="guaA"
                     /locus_tag="ACFI8B_001618"
     CDS             11333..12898
                     /gene="guaA"
                     /locus_tag="ACFI8B_001618"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC3834795.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNILDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYYYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            12960..13706
                     /gene="fabG"
                     /locus_tag="ACFI8B_001619"
     CDS             12960..13706
                     /gene="fabG"
                     /locus_tag="ACFI8B_001619"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC3834796.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            13771..14256
                     /locus_tag="ACFI8B_001620"
     CDS             13771..14256
                     /locus_tag="ACFI8B_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC3834797.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPAVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(14476..15270)
                     /locus_tag="ACFI8B_001621"
     CDS             complement(14476..15270)
                     /locus_tag="ACFI8B_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC3834798.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            15374..16099
                     /locus_tag="ACFI8B_001622"
     CDS             15374..16099
                     /locus_tag="ACFI8B_001622"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC3834799.1"
                     /translation="MPRFYLPENLSVGQTVDLPDNIVRHLNVLRIRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
BASE COUNT         4145 a         5099 c         3810 g         3220 t
ORIGIN      
        1 agcaaccgct tccgccgaag ccgcgctcgc gcccgcaacg ccgattaccg taacgaccga
       61 cacggttcaa gccgttattg atgaaaaaag tggcgacctg cgccggctga ccctgctcaa
      121 atacaaagca accggcgacg aaaacaaacc gttcgtcctg tttggcgacg gcaaagaata
      181 cacctacgtc gcccaatccg aacttttgga cgcgcagggc aacaacattc tgaaaggcat
      241 cggctttagc gcaccgaaaa aacagtacac cctcaacggc gacacagtcg aagtccgcct
      301 gagcgcgccc gaaaccaacg gactgaaaat cgacaaagtc tataccttta ccaaagacag
      361 ctatctggtc aacgtccgct tcgacatcgc caacggcagc ggtcaaaccg ccaacctgag
      421 cgcggactac cgcatcgtcc gcgaccacag cgaacccgag ggtcaaggct actttaccca
      481 ctcttacgtc ggccctgttg tttatacccc tgaaggcaac ttccaaaaag tcagcttctc
      541 cgacttggac gacgatgcga aatccggcaa atccgaggcc gaatacatcc gcaaaacccc
      601 gaccggttgg ctcggcatga ttgaacacca cttcatgtcc acctggatcc tccaacctaa
      661 aggcggccaa agcgtttgcg ccgcaggcga ctgccgtatc gacatcaaac gccgcagcga
      721 caagctgtac agcgcaagcg tcagcgtgcc tttagccgct atccaagccg gcgccaaagc
      781 cgaaactgcc gtcaacctgt atgccggtcc gcaaaccaca tccgttatcg caaacatcgc
      841 cgacaacctg caactggcaa aagactacgg taaagtacac tggttcgcat cgccgctctt
      901 ctggctcctg aaccaactgc acaacattat cggcaactgg ggctgggcaa tcgtcgtttt
      961 gaccatcatc gtcaaagccg tactgtatcc attgaccaac gcctcctacc gttcgatggc
     1021 gaaaatgcgt gccgccgcac ccaaactgca gaccatcaaa gaaaaatacg gcgacgaccg
     1081 tatggcgcaa cagcaagcga tgatgcagct ttacaaagac gagaaaatca acccgctggg
     1141 cggctgtctg cctatgctgt tgcaaatccc cgtcttcatc ggcttgtact gggcattgtt
     1201 cgcctccgta gaattgcgcc aggcaccttg gctgggctgg attaccgacc tcagccgcgc
     1261 cgacccctac tacatcctgc ccatcattat ggcggcaacg atgttcgccc aaacctatct
     1321 gaacccgccg ccgaccgacc cgatgcaggc gaaaatgatg aaaatcatgc cgttggtttt
     1381 ctccgtcatg ttcttcttct tccctgccgg tttggttctc tactgggtgg tcaacaacct
     1441 cctgaccatc gcccagcagt ggcacatcaa ccgcagcatc gaaaaacaac gcgcccaagg
     1501 cgaagtcgtt tcctaaatgc cgcagcatga aaaatgccgt ctgaaacctg ttcagacggc
     1561 atttttattg ttcaccccct atcggggcgg aaatcttcaa cccgcataca tcacaaaaat
     1621 cgtcgggcgt tttttcagat tgggcatttc cttcatcctg cgccaatccg caaccgtttt
     1681 actgaccacc aactgagtcg gcaacgtcaa atccgtagcc gtacacaagc gcgtttcagg
     1741 atgcaggttt tccaccgcat cggcaagcag cgcatcattg cggtaaggcg tttcaataaa
     1801 aatctgcgtc tcgccgcact gccgcgaacg ttgctccaaa gcattcaaac tctgaatgcg
     1861 ctcatttttt tcagacggca gataaccatt aaacgcaaag ttctgcccat tcgcacccga
     1921 agccatcagc gccagcagca ggctggacgg gccgaccaaa ggccgtactt caaaaccgtg
     1981 tttatgcgcc aatgccacca aattcgcgcc cggatcggca acagccgggc aacccgcctc
     2041 gctgacaatc cccatactgc gcccttcttg caaaggtttc agcaattccg gcaaagtctt
     2101 caaatccgtg tgttcattca acgtttgcag attcagttcg cgtatcggcg tcgttatccc
     2161 caaatgcttc aaatgcgcac gcgccgtttt ttccgcttcc acgacaaaat ccgtcagccc
     2221 gacaaccgcc cgttgttcat gctgcaacag gcacggcgtg tcaggcgtac ccaaaggcgt
     2281 aggaatcaaa tacaaaacag gagacatcat tccctcactc atcggttaaa aatgccgtcc
     2341 gaacccttca gacggcataa acgggcagtt acaaaacatc cacgccctcg tttttcaaaa
     2401 aatcgaccag acggaaaacc ggcagaccaa tcaaggcatt cggatcggta ctttcaatcc
     2461 gttcgatcaa cagcgcgccc aagccctcgc ttttcgccgc acacgaacaa taaaccgcat
     2521 ccggctcgcg ctccaaatag cggaggatgt gcagctcgtc caactgcctc atcacgacca
     2581 ccgtcttatc gatatgccga tgcatcctgc ccgtaaccgt atttagcaga acgaccgcac
     2641 tgtaaaactc aatctccctg ccgctcaaat gcatcaacat cttttgcgcg ttggcaaggt
     2701 tcatcggctt gccccactgc ctgccgtcgc accacgccac ctggtccgca ccgacaatca
     2761 acgccccggg gaaacgcccg gccaacgacc gcgccttacc ctcggcaagg cgcaatgccg
     2821 tctgaggggc ggattccccc aacatcggcg tttcgtcaaa atcgggggat gccgcctgaa
     2881 aggcaatgcc gagcctttcc atctgttcgc ggcggaaaac cgaactcgta cccaaaacca
     2941 aaggcagttc caaacccatc ccatcctcct taccgttgaa aacacgcccg aaggggcggt
     3001 aaaatccagc catgcgccga aacacggaca cccgccttcg gcgtaccgca acatttttct
     3061 taaaaatatt gacgttagaa catctaaatt atatcatacc ccgtttatgt cagaccctaa
     3121 tttgattgac ccggaaattt ttgccgccga aaggcagaac ctgcaaggca gttttctgct
     3181 ggaagaattg gacgagcgag tcagtttgca cgattatccc gccgacaggc ggaccaaaat
     3241 atcgtttaca ctgaccggcg gtcgcgaccg gctgcaacgc ctgttcctcg acctgaacgt
     3301 caaagccgat atgcccctga tttgccagag atgtatcaaa cccatgccgt tcatgctcga
     3361 tgaaagcagc cgtatcgtcc tgttttccga cgaagagtcc ttggacgaat ccatgcttgc
     3421 cgacgaagaa ctcgaaggca tactgattga aaaagaactc gacgtgcgcg cattggtaga
     3481 agaccaaatc ctgatgtccc tgcccttttc gccgcgacac ggacactgcg gcaataccct
     3541 tccggaatcc gccaaccaag acaaacccaa cccctttgct gttttggcgg gtttgaaaag
     3601 cagttaatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
     3661 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcactgaccg cgcccgcact
     3721 gtttgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
     3781 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     3841 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     3901 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
     3961 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
     4021 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccggcgtcaa
     4081 atacgatgcc atgatcgcta tcggcggcgg cggctttatt ccggcacgca tgctgcgctg
     4141 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acagcgaagg
     4201 acaagttacc gaagaagtca aaaaagtcca atggctagac cccgtccccg aagtcctgcg
     4261 gggcaaaaac gtgctcgtcg tcgatgaagt ggacgacagc cgcgtaacca tggagttctg
     4321 cctgaaagaa ctgctcaagg aagacttcga cactgtcggc gtcgccgtcc tgcacgaaaa
     4381 aatcaaagcc aaagcaggca aaatccccga aggcattccc tatttcagcg gcattaccgt
     4441 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
     4501 tgccgaagcc ggccgaggct aaccctttca gacggcatat tttccgaacc gatgccgtct
     4561 gaagcccgca cgacccctgc cgcagaccga aaatctaccg gagaaacccc tatgattaca
     4621 ttggccgtag atgccatggg cggcgaccaa ggacttgccg ttaccgtacc cggcgcaacc
     4681 gcattcctcc aagcacaccc cgatgtccgc ctgattatga ccggcgacga aacgcagctc
     4741 cgccaagccc tgaacgcggc aggcgcgccg atggaacgca tcgacatctg ccataccacc
     4801 caggttgtcg gcatggatga atcccctcag tccgccctaa agaacaaaaa atactcctcc
     4861 atgcgcgttg ccatcaacca agttaaagaa ggcaaagccc aagccgccgt atccgcaggc
     4921 aacacgggcg cgctcatggc aaccgcacgt ttcgtcctca agaccatacc cggcatcgaa
     4981 cgccccgcca tcgccaaatt ccttccttcc gacaccgacc acgttaccct cgccctcgac
     5041 ctcggcgcaa acgtcgactg cacacccgaa cagcttgccc aatttgccgt catcggcagc
     5101 gaactcgtcc acgcactcca tcctcaaaaa ggacagccgc gcgtcgggct ggtcaacgtc
     5161 ggcacagaag acatcaaagg tacggatacc gtcaaacaaa cctacaaact gctgcaaaac
     5221 agcaaactca actttatcgg caacatcgaa agcaacggca tcctgtacgg cgaagcagat
     5281 gtcgtcgtcg ccgacggctt tgtcggcaac gtcatgctca aaaccatcga aggcgcagtc
     5341 aaattcatga gcggagccat ccgccgcgaa ttccagagca acctgttcaa caaacttgcc
     5401 gccgttgccg ccctacccgc cctcaaaggc ttgaaaaaca aactcgatcc gcgcaaattc
     5461 aacggggcca tcctgctcgg gctgcgcggc atcgtcatca aaagccacgg cggcacagac
     5521 aaaaccggtt tccgctacgc actcgaagaa gcctaccacg aagccaagtc cgccggcctt
     5581 tccaaaatcg aacaaggcgt tgccgaacaa ctcgccgcac tggaagccgc ccaaaacgaa
     5641 accgccgcca gtctgtaaca cgcacgatgc cgactgaacg cccccgcccc tttcagacgg
     5701 catccggcac ccgtccaaac ctgcgggcgc ggacggcgat gcgcctgtcc ggcacttccc
     5761 aaatacttcc ctgtaaaata aggagtattt gaaaaatgaa gacattagaa aaacggatga
     5821 aagctctaga caaacggatg atgaagttcg gaaaatccct tgaaggcagg cttgatgccc
     5881 gtctgattga atccgcattg gattatattc attattcgga acgttttttg gcttttgaca
     5941 tcctgtgtac ttatatcgaa gatttcgata tccggctgac ggaacaagaa tcccgggaaa
     6001 tttcttttat caacaaggaa tttgagatag aaagcacgtc cgattaacca ataaagccaa
     6061 tgatttgata agaatgaaaa catcgacggt cgtttttggc ggatttttta tggcagacaa
     6121 cggggagcga atccaaatcc ccgttttgga aaatcctgac attagggaaa tcaatcgctt
     6181 tttttccgta tcaaattttg agaaaaaagc cggcgtcctt gttttcagaa tcatccccga
     6241 gccggaattt ggcaataccg aattaactgt ctattttgaa aaaggatatt atagtggatt
     6301 aacaaaaacc ggtacggcgt tgccccgcct tggctcaaag ggaacgattc cctaaggtgc
     6361 ccaagcacca agtgaaccgg ttccgtacta tccgtactgt ccgcggcttc gccgccttgt
     6421 cctgattttt gttaatccac tatatttgcc aataattcaa acaattttag aaggcgggga
     6481 tattaaagta aaaaacctta aaacagaaaa ttacagcggg aaggcaaggg aaatcttggg
     6541 agattgctac cctattgaac atatctctaa aaatatttct acaattcaaa atatcatttc
     6601 cgaatttatc agacaaaaac aaacaccggc accaaataat ttgatggaaa cccaacaatc
     6661 aagatgccgt ctgaagcagc ttgggctttc agacggcatt tccttcgctt aaaacagcgt
     6721 atcggcaacc ccgccccgcc cgtccacggc aatccgcatc tgaaaaccat ctgtatgcca
     6781 aaccacaccc ccatccctgt ttccgtcatt tgcaccctgc ccgtattggg caatcatcgg
     6841 tttttcgctt acaatagccg aatctgaacc aactctctaa aaaggccgtt cccatgcagt
     6901 atgcaaaaat ttccggcacg ggcagctatc ttcctgccaa ccgcgtcagc aatgacgacc
     6961 ttgcccaaaa ggtagatacc tctgacgagt ggattaccgc gcgtacgggc atcaaattcc
     7021 gccatattgc agccgaaaac gaaaaaacca gcgatcttgc agccgaagcg gcgcggcgcg
     7081 ctttggcaga tgccaaatta aatataaacg acattgattt gattatcgtg gcgacggcaa
     7141 cgccggatat gcagtttcct tctaccgcca ccatcgtgca gcaaaaattg ggcatcacca
     7201 acggctgccc cgcgtttgac gtgcaggcgg tgtgcgcagg ctttatgtac gcgctgacta
     7261 cggcaaatgc ctacattaaa agcggtatgg cgaaaaacgc gctggtcatc ggcgcggaaa
     7321 ccttcagccg catcgtcgat tggaacgacc gcacaacctg cgtgctgttc ggcgacggcg
     7381 cgggcgcggt ggttttaagc gcgtccgaca aaccgggcat catccacagc aaactcaaag
     7441 ccgacggcaa ttatctgaaa ctcttaaacg tccccgggca aatcgcctgc ggcaaagttt
     7501 ccggttcgcc gtacatttcg atggacggtc ccggcgtgtt caagtttgcc gtcaaaatgc
     7561 tgtccaaaat cgccgatgac gttatcgaag aagcaggtta caccgccgaa caaatcgact
     7621 ggattgttcc ccatcaggca aaccgccgca ttatcgaatc gaccgcgaaa catttgggtt
     7681 tgagtatgga caaagtcgtc ctgaccgtcc aagaccacgg caacacgtcc gccgcatcca
     7741 ttccgctggc tttggacgcg ggcatccgca gcggacaaat caaacgcggt caaaacctgc
     7801 tgctcgaagg cataggcggc ggtttcgcgt ggggcgcggt gctgttgcaa tattgaaacc
     7861 ggtgccgtct gaaacaggct ttcagacggc atttcccata tcatgaagcg gcaggctttc
     7921 ttcaaaccga tggcgtgtgc ggcatttctg tccgccgttt cgctgcgcct ccccgtattg
     7981 ggcgcgtgtt acgcaatatt gtccctctat gcgtttgcac tttacggcat cgacaaacgg
     8041 cgtgccgtgc ggggaaaacg ccgcattccc gaacaccgcc tgctcctgcc tgccttgttc
     8101 ggcggttgga cgggcgcata cttgggtagt aggatgttca ggcataaaac ggcgaaaaag
     8161 cgttttgttg tgctgttccg tctgactgtt tcgggcaatg tcctggcgac ctgcatcctg
     8221 attgattatt tcgttccgcc cgaacttttt gtaaaactcg ggcaacatct ctgacaatcc
     8281 aacccaacaa aggaatacta tgtctttcgc attcttcttc cccggacaag gttcccaaag
     8341 cctcggcatg atgaacggct ttgccgaaca cgccatcgtc aaaaacacat ttgacgaagc
     8401 ctccgccata ttggggcagg acttgtgggc gatgattaac ggcagcgatg ccgaaatcat
     8461 cggtcaaaca gtcaacaccc agcccattat gctcgccgcc ggcgttgccg tttaccgcgc
     8521 ctatttggaa gtgggcggca aaacgcctgc cgccgttgcc ggacacagcc tcggcgaata
     8581 caccgcgctt gtcgccgccg aagcattgga ttttgccgat gccgtcaaac tcgtccgcct
     8641 gcgcgccgaa ctgatgcagt ccgccgtacc gcaaggcgta ggcgcaatgg cggccatttt
     8701 gggtttggaa gatgaacagg tcaggcagat ttgtgccgaa tccgcacagg gcgaagtggt
     8761 cgaagccgtc aacttcaact cgcccggaca agtcgtgatt gcaggcaacg ccgccgccgt
     8821 cggacgcacc atggctgccg ccaaagaagc cggagccaaa cgcgccctgc cgctgccggt
     8881 atccgttcct tcccattgca gcctgatgaa acccgccgcc gacaaacttg ccgaagcact
     8941 caaaaccgtt gaaatcaagc agccgcaaat ccgtgtcatc cacaacgccg acgttgccgc
     9001 ctacgatgat gccggcaaaa tcaaagacgc gctcgtccgc cagctttaca gccccgtgcg
     9061 ctggacggaa accgtcaacg ccctcgtttc agacggcatt gccgaatccg ccgaatgcgg
     9121 cccgggcaaa gtgttggcgg gcttggcaaa acgcatcaac aaggccgccg cgtgcagcgc
     9181 actgaccgat gccggacaga ttaccgcctt tatcgaagcg cactgacttc gttccgcaaa
     9241 aagcagcctg ccctcttcag gctgcttttc acgtccgaac ggcaaacagc cgcatatttg
     9301 cgctataatc catccctacc aaaccaccga cagcggctgc cgttgcagtt cccgccctac
     9361 cgatatgata gaaaaactga ctttcggact gtttaaaaaa gaagacgcgc gcagctttat
     9421 gcgcctgatg gcgtacgtcc gcccctacaa aatccgcatc gttgccgccc tgattgccat
     9481 tttcggcgtt gccgccaccg aaagctatct tgccgccttc atcgcccccc tgatcaacca
     9541 cggcttttcc gcgcccgccg cgccgcccga tctgtccgcc gccgccggca tcctttccac
     9601 cctgcaaaac tggcgcgaac agtttaccta tatggtttgg gggacggaaa acaaaatctg
     9661 gaccgtcccg ctcttcctca tcatcctcgt cgtcatccgt ggcatctgcc gctttaccag
     9721 cacctatctg atgacttggg tctccgtgat gaccatcagc aaaatccgca aagatatgtt
     9781 tgccaaaatg ctgacccttt cctcccgcta ccatcaggaa acgccgtccg gcaccgtact
     9841 gatgaatatg ctcaacctga ccgaacagtc ggtcagcaac gccagcgaca tcttcaccgt
     9901 cctcacgcgc gacacgatga tcgttaccgg cctgaccatc gtcctgcttt acctcaactg
     9961 gcagctcagc ctcatcgtcg tcctgatgtt ccccctgctc tccctgctct cgcgctacta
    10021 ccgcgaccgt ctgaaacacg tcatttccga ctcgcaaaaa agcataggca cgatgaacaa
    10081 cgtgattgcc gaaacccatc agggacaccg cgtcgtcaag ctgttcaacg ggcaggcgca
    10141 ggcggcaaac cggttcgacg cggtcaaccg caccatcgtc cgcctcagca aaaaaatcac
    10201 gcaggcaacg gcggcacatt ccccgttcag cgaactgatt gcctcgatcg ccctcgccgt
    10261 cgtcatcttc atcgccctgt ggcaaagcca aaacggctac accaccatcg gcgaatttat
    10321 ggcattcatc gtcgcgatgc tgcaaatgta cgcccccatc aaaagccttg ccaacatcag
    10381 catccctatg cagacgatgt tcctcgccgc cgacggcgta tgtgcatttc tcgacacccc
    10441 gcccgaacag gacaaaggca cgctcgcgcc gcagcgtgtc gaagggcgca tcagcttccg
    10501 caacgtcgat gtcgaatacc gttcagacgg catcaaagcc ctcgacaact tcaacctcga
    10561 catcagacaa ggcgaacgcg tcgccctggt cggacgttcc ggcagtggca aatccaccgt
    10621 cgtcaacctt ctgccccgct ttgtcgaacc gtctgccggc aacatctgca tagacggtat
    10681 cgacatcgcc gacatcaaac tcgactgcct gcgcgcccaa ttcgccctcg tctcccaaga
    10741 cgtattcctg tttgacgaca ccctgtttga aaacgtccga tacagccgtc ccgacgcggg
    10801 cgaagccgaa gtcctgtccg ccctccaagc cgccaacctg caaagcctga tcgacgcatc
    10861 cccgctcgga ctgcaccagc ccatcggatc gaacggcagc aacttgtccg gcggacagcg
    10921 gcaacgcgtc gccatcgccc gcgccatttt gaaagacgcg ccgatattat tattggacga
    10981 agccaccagc gcattggaca acgaatccga acgcctcgtc caacaagcgc tcgaacgcct
    11041 gatggaaaac cgcaccggca tcatcgtcgc ccaccgcctg accaccgtcg aatccgccga
    11101 ccgcatcatc gtcatggatg gcggcaaaat catcgaacaa ggcacacacg atcaactcat
    11161 gttccaaaac ggctactaca ccatgctgag gaacatctcc ggaaaagata ccgccgccgt
    11221 tcagacggca taaacaaaat gccgtctgaa atggtacaat catccaaatc ccttcagacg
    11281 gcactatatc cgccgaccca tccgattatc ttcaaccacc gtaaaaccca ttatgaccca
    11341 agacaaaatc ctcatcctcg acttcggttc gcaagttacc cggctgatcg cccgccgcgt
    11401 gcgcgaagca cacgtttact gcgagctgca ttccttcgat atgcctttgg acgaaatcaa
    11461 agccttcaac cccaaaggca tcatcctttc cggcggccct aattctgttt acgaatccga
    11521 ctatcaagcc gataccggta tttttgattt gggcattccg gttttgggca tctgctacgg
    11581 catgcagttt atggcgcacc acttgggtgg cgaagtgcag cccggcaacc agcgcgaatt
    11641 cggttacgcg caagtcaaaa ccatcgacag cggactgaca cgcggcattc aagacgacgc
    11701 gcccaacata ctcgacgtat ggatgagcca cggcgacaaa gtgtccaaac tgcccgacgg
    11761 tttcgccgtc atcggcgaca ctccgtcctg cccgattgcc atgatggaaa acaccgaaaa
    11821 acaattctat ggcatccagt tccaccccga agttacccac accaaacaag gccgcgccct
    11881 gttgaaccgc tttgtcttgg atatttgcgg cgcgcaaccg ggctggacga tgcccaacta
    11941 catcgaagaa gccgttgcca aaatccgcga acaagtcggc agcgacgaag tgattttagg
    12001 tctgtccggc ggcgtggact cttccgtagc cgccgcgctg attcaccgcg ccatcggcga
    12061 ccaactgacc tgcgtgttcg tcgatcacgg tttgttgcgc ctgaacgaag gcaaaatggt
    12121 gatggatatg ttcgcccgca acttgggtgt gaaagtgata cacgtcgatg ccgaagggca
    12181 gtttatggcg aaactcgccg gcgtgaccga ccctgagaaa aaacgcaaaa tcatcggcgc
    12241 ggaatttatc gaagtatttg atgccgaaga gaaaaaactc accaacgcca aatggctggc
    12301 gcaaggcacg atttaccccg acgtaatcga atccgccggt gcgaaaacca aaaaagccca
    12361 cgccatcaaa tcccaccaca acgtcggcgg tctgcctgaa aatatgaagc tcaaactgct
    12421 tgagcccttg cgcgacttgt tcaaagacga agtgcgcgag ttgggcgtgg ctttgggcct
    12481 gccgcgcgaa atggtgtacc gccacccctt cccgggcccc ggtttgggtg tgcgcatctt
    12541 gggcgaagtg aaaaaagaat acgccgactt gctgcgtcag gcggacgata ttttcatcca
    12601 agaattacgc aatactaccg acgaaaacgg cacgtcttgg tatgacctga ccagccaggc
    12661 atttgccgtg ttcctgcccg tcaaatccgt cggcgtgatg ggcgacggcc gcacttacta
    12721 ctacgtcgtc gcactgcgcg cagtcatcac cagcgacttt atgactgcac actgggcaga
    12781 gctgccatac tcactgctcg gccgcgtgtc caaccgcatc atcaacgaag tcaaaggcat
    12841 caaccgcgtg gtgtacgatg tcagcggcaa accgcccgcc accatcgagt gggaataaac
    12901 agcaaacagg gctgcaccgt ccggcgcagt ccttcgatta tcggaaaaaa ggaaaaaata
    12961 tgagcacaca agatttaagc ggcaaaatcg ctttggtaac cggcgcatcg cgcggcatcg
    13021 gcgcggcaat tgccgacaca ctggcggcag ccggtgccaa aatcatcggt acggcaaccg
    13081 gcgagagcgg tgcggcggcg attagcaaac gtctggcaca atggggcggc gaaggccgcg
    13141 tattaaattc cgccgaaccc gaaaccgtcg aaaacctgat tgccgacatc gaaaaaacgt
    13201 tcggcaaact cgacattctg gtcaacaacg ccggcatcac ccgcgacaac ctgttgatgc
    13261 gtatgaaaga agaagagtgg gacgacatca tgcaggtcaa cctcaaatcc gtgttccgcg
    13321 cttctaaagc cgtcttgcgc ggcatgatga agcagcgcgc cggccgcatc atcaacatca
    13381 cctccgtcgt cggcgtgatg ggcaatgccg ggcaaaccaa ttatgccgcc gcaaaagcgg
    13441 gcttgatcgg ttttgccaaa tctatggcgc gcgaagtcgg cagccggggc attaccgtca
    13501 actgcgtcgc cccgggcttt atcgataccg atatgacccg cgccctgccc gaagaaaccc
    13561 gccaaacatt taccgcccaa accgccttgg gcagattcgg cgacgcgcaa gacatcgccg
    13621 atgcggttct gttcctcgct tccgatcagg cgaaatacat caccggtcaa acgctgcacg
    13681 tcaacggcgg tatgctgatg ccctaacgga caggcttttc aaccatgccg tctgaagccc
    13741 tttcagacgg catttgcatt cccaaacaaa atgaacactc accaccacct gccctgcccc
    13801 tgcggttcag gcacaagcta tgccggctgc tgccgaccgc ttcatctgcg ccaaatcctg
    13861 ccgcccaccg ccgaagccct catgcgttcg cggtacggcg cgtatgtgct gcaccttatc
    13921 gactacatca tcgctactac cgttccggcg caacaaacat tcctcgatgc cgccgaactg
    13981 atgcagtgga gcagagaagc cgaatggctg ggcttgaatg tcatcgcaca ccgcaacctc
    14041 ggcaaacagc acgcccaagt cgaatttgaa gcctacttcc aagacggcgg aaaccgaaca
    14101 gtgcatcacg agctgtcggc attcgtgaaa atagcggagc aatggtattt catcgatccg
    14161 gctgttccgc tgcctaccat gaaacagccc tgcatttgcg gttcgggcaa aaaattcaaa
    14221 gcctgctgcg gcaaatatct gcaacctgtc gcataaaaat gccgtctgaa cgttcagacg
    14281 gcattttcaa cgcgcaaaaa aaaaaccatt cataccaagg gtaagtatga atggtcaata
    14341 cattgcggga aaacatctta cttgctgcac tgccgaaaag ggagaaacgg cagtgttaac
    14401 cagcaaaagc attgtacctt tattaatgtt aaaaaacatt aattttgaaa atatattaac
    14461 gaatatatct ggaacctact ggttttcccg tatccacccg acccagcgtt caaacagctt
    14521 cggttcgagc gcggcgacga ccgagcgttt gaacacgtgt tcaccactcc aaaacccgtc
    14581 gccttccaaa gtcgtcagcc tgccgcccgc ctcctcgaaa atcaacgcac ccgcagcata
    14641 atcccacagc ttctgcccgc cgtggacata aacatcataa cgcccgcacg ccagataaca
    14701 ccagtccagc gtgctgctgc ccatactgcg tatcgtcccg aaaggcgcga gcgtactcat
    14761 gcggctggaa agtttgcccg aacgcagata tttgatttcc acgcccgcaa tcgcctcatt
    14821 gagtttttta tccacgagac gcaaaggcag gcgtgtccca ttaagaaacg caccctgccc
    14881 gcgttcggca taaaaacatt cgccgctgac cggattgtag attacgccca attcggcgcg
    14941 cccgttgcgg acaaacgcca ccgacacggc aaaatgcggc agcccgttga caaaattgtt
    15001 cgtcccgtct atcggatcga caatccacag ccccttttcc cccgaatatt gttcccacaa
    15061 agccgactgt tcctgccgcg acatttcctc gcccaacatc gggcaatcga tcagaagcgg
    15121 caacgcggcg gcaaatgccg tctgcgcggc aatgtccgcc tcgctcaaca tcgaaccgtc
    15181 ttccttgcgg cgggacggcg tattcaaaaa acgcggcata atttcggttt gcgcgatatg
    15241 gcgcacgact ttctgcaaac ggtgtaacac ttcctactgt cctcatattt tgaacttgcg
    15301 gcgcgcgaac gtataatgtc cgtttccatc acgccgctgc gacggattat aaccgtccga
    15361 accgccaaaa actatgccca gattctacct gcccgaaaac ctttccgtcg gacaaaccgt
    15421 cgaccttccc gacaacatcg tccgccacct caacgtcctg cgcatccgcc ccaacgaaaa
    15481 catcaccctc ttcgacggca aaggcaaggc acataccgca cggctgaccg ttttggaaaa
    15541 acaccgcgcc gaagccgaaa tcctgcacga agacacaacc gacaacgagt ccccgctcaa
    15601 catcacgctc atccaatcca tttcctccgg cgatcgcatg gatttcaccc tgcaaaaaag
    15661 cgtcgaactc ggcgtaaccg ccatacagcc cgtcatcagc gaacgctgca tcgtccgcct
    15721 cgacggagaa cgcgccgcca aacgcctcgc acgctggcag gaaatcgtca tctccgcgtg
    15781 cgaacaaagc ggcaggaaca ccgttccccc cgtactgccc atcatcggct accgtgaagc
    15841 actcgacaaa atgccgtctg aaagcaccag gctgattatg agcatcaacc gcgcctgcaa
    15901 actcggcgac atccgccacc cgtccggcgc aatcgtcttt atggtcgggc ccgaaggcgg
    15961 ctggacagaa caggaagaac aacaggcatt tgaagccggc tttcaggcgg ttacactcgg
    16021 aaaacggatt ttacgcacag aaaccgcccc actcgccgcc atcgccgcga tgcagacgct
    16081 ttggggcgat tttacctagc agatgaaaaa acacgggatt ttatccgaaa aagcaacaat
    16141 ccccccgccg tcattcccgc gaaagcggga atctagaaat ttaacgttgc ggtgatttat
    16201 cggaaatgac tgaaacccaa cgaaccggat tcccactttc gcgggaatga cgaagtggaa
    16261 gttacccgaa actt
//
LOCUS       DAXFWM010000056        16034 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000056 DAXFWM010000000
VERSION     DAXFWM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.056"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(315..737)
                     /locus_tag="ACFI8B_001623"
     CDS             complement(315..737)
                     /locus_tag="ACFI8B_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC3834800.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            complement(855..1526)
                     /locus_tag="ACFI8B_001624"
     CDS             complement(855..1526)
                     /locus_tag="ACFI8B_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC3834801.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1542..2168)
                     /gene="purN"
                     /locus_tag="ACFI8B_001625"
     CDS             complement(1542..2168)
                     /gene="purN"
                     /locus_tag="ACFI8B_001625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC3834802.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2275..3093)
                     /locus_tag="ACFI8B_001626"
     CDS             complement(2275..3093)
                     /locus_tag="ACFI8B_001626"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3834803.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPAPASEPAAASAAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            complement(3353..3793)
                     /locus_tag="ACFI8B_001627"
     CDS             complement(3353..3793)
                     /locus_tag="ACFI8B_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC3834804.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3856..5262)
                     /locus_tag="ACFI8B_001628"
     CDS             complement(3856..5262)
                     /locus_tag="ACFI8B_001628"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC3834805.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            complement(5265..5447)
                     /locus_tag="ACFI8B_001629"
     CDS             complement(5265..5447)
                     /locus_tag="ACFI8B_001629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834806.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            5436..6551
                     /gene="lptF"
                     /locus_tag="ACFI8B_001630"
     CDS             5436..6551
                     /gene="lptF"
                     /locus_tag="ACFI8B_001630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC3834807.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRNREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6548..7618
                     /gene="lptG"
                     /locus_tag="ACFI8B_001631"
     CDS             6548..7618
                     /gene="lptG"
                     /locus_tag="ACFI8B_001631"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC3834808.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7933..10518
                     /gene="acnB"
                     /locus_tag="ACFI8B_001632"
     CDS             7933..10518
                     /gene="acnB"
                     /locus_tag="ACFI8B_001632"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC3834809.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10660..11655
                     /locus_tag="ACFI8B_001633"
     CDS             10660..11655
                     /locus_tag="ACFI8B_001633"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC3834810.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKKTGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11774..12787)
                     /gene="ilvC"
                     /locus_tag="ACFI8B_001634"
     CDS             complement(11774..12787)
                     /gene="ilvC"
                     /locus_tag="ACFI8B_001634"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC3834811.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12867..13160)
                     /locus_tag="ACFI8B_001635"
     CDS             complement(12867..13160)
                     /locus_tag="ACFI8B_001635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC3834812.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            complement(13227..13718)
                     /gene="ilvN"
                     /locus_tag="ACFI8B_001636"
     CDS             complement(13227..13718)
                     /gene="ilvN"
                     /locus_tag="ACFI8B_001636"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC3834813.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13729..15456)
                     /gene="ilvB"
                     /locus_tag="ACFI8B_001637"
     CDS             complement(13729..15456)
                     /gene="ilvB"
                     /locus_tag="ACFI8B_001637"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC3834814.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
BASE COUNT         3750 a         4374 c         4078 g         3832 t
ORIGIN      
        1 taattcaata aattaatata tggcttaaaa taacgggatt cccgccttcc gcccgcccgc
       61 aaaatcaggc gggcatcatt ttaaaacgcg gcatttaaaa tttgaccgaa aattgttgac
      121 aatccggaat caagcctgca caataccccg acaagtccaa gtattataaa ggctgaataa
      181 agaggaaaca gcaggcagat atattcggga ggtgcagtcc gaatatatct gcttttttat
      241 gcgcctccgg attgcctgcc gcacctttcc ccttcagacg gcatcagccg tttccccata
      301 atgccgcccg atgcctattt ctccgccccg gcaatttcaa aactgtgggt aatctttgcc
      361 gctttgccca acataatcga agccgaacag tatttttcgg cagacatctg aacggcgcgc
      421 tcaatggccg attctttcaa atcatgcccg attaccttga aatggatgtg gatttcggta
      481 aacacgcgcg gcgcatcgtc cgcccgtttc gccgtaaccg tcgcacggca gtcagtcact
      541 ttctgacgct gtttttcggc aatcatcacc acatcgatgc tcgaacagcc cgccaccccc
      601 aacagcagca tttccaaagg gctgggcccg cgcttggccg cgccttctgc cgccgatccc
      661 tccataacga cgctgtgccc gccttccgcc gtgccgacaa aacacattcc gtctatccat
      721 tttgatgtaa cctgcatggt gtcattcccg aaaatagcgt taaaaccgct ttgcatatgg
      781 cgttattgta aacaatttca agcggcttat acagaaatat ggacaaaacg gcaaaaaacc
      841 gcctgaaaac cgatttacgg tttggcggcc tgtccgttga tctgcaccga tttgagcttc
      901 agcgtatagg ttttgccgtc gtcggtatag ccgatttgtg ccggaatatt gttcagggac
      961 ggtgcgaaga aatacgttac cgtatcgtcg ccgcgccgca cccgatattt gacgacttcg
     1021 gtttccacgc cgcctatgct gtattttccc gtacccgcct tattcaggcc gccgacggaa
     1081 taaagttttt tgccgttggt gattttcaga cccgggggga gtttcgcgtc atttgccgcc
     1141 aactgccagg caagcgtgaa caaatccata gccttggggc tttgctcggt tttgctctcg
     1201 cccgctttgc cgtaggttac gctgccgtcg gcgaatttgg cttccgcata cagtttgccc
     1261 ctgcgtatgt ctttatagta ggcagggtgc agggtattgc cgacaaccgt accgccggac
     1321 tcgaaacgga tattgtatag cggcacttta atcgtcgaaa cgattttgta agcattgccg
     1381 ctgcgttcaa atgtcatcgt ggcgggaatg ccgtagctgc cggaatagtg cagcacggcg
     1441 gattggggta gccttgccgc atacgcgcac ggcagggcgg cggacaaaat ggcggcggaa
     1501 aatatatttt taaaagtctt catcatttgc tcccgcccgg tttacgccgt caggaaacgg
     1561 gcggcatcgg cgttttccga atttctgaca cggtttccct cgataatcag gcggccggcg
     1621 gcaaaatcgg caacggcttt cggatagagt ttgtgttcga cagccaaaac ccgggcggca
     1681 acatcgtctg ccgtatcgcc gtcaagtatc ggcacaatcc cttgcgatac aatctggccg
     1741 caatccagct cggccgtaac gaaatgaatc gtacagccgg caacgcggca acctgcttcc
     1801 aaagcgcgtt cgtgcgtatg aagcccggtg aacgagggca ggatggacgg atggatgttc
     1861 atcagcctgc cttcgtaatg ggcgcagaac tcaggggtca gaatccgcat aaaacctgcc
     1921 aaaaccacca aatcgggttg atatgcgtcg attttttcca tcatggccgt atcgaaggca
     1981 agtcgtgatt caaagttttt atgattcagg ctgtcggtcg ggatgccgcg ttcggccgcc
     2041 cattgcaaac cggcagccgt ttcgctgttg ctcaacacgg cggcaatgcg gacgttggga
     2101 atggcggcat tgacgattgc ctgcatattg ctgccgcgtc cggaaatcag gatgacgatg
     2161 tttttcataa tggtgcgctt ttgaaaggga tgccgtctga accgctgttt ggtggcttca
     2221 gacggcattt gccgtaaaaa tgcccgaaaa cctgtttcgg gcatggattc ggacttaatt
     2281 tacttttttg atgtcgactt gatccggctg cttggcgggc gcgttttcgg gtgcgccgat
     2341 tttgaccagt ttcacgtcaa ataccaaagt ggcgttcgga ccgatttttt cgcccgcacc
     2401 ctgttcgcgg taggcaaggt tggacgggat gtagaacgtg gcttcgccgc cttctttcag
     2461 aagccgtacg ccttcggtcc aacccggaat cacttggctc aaagggaagg tggccgggcc
     2521 gccgttggct ttgctgctgt cgaatacggt accgtcaatc aggcggcctt cgtattccac
     2581 ggtaacgatg tcgtcttttg tcggctgttt gccttcaccc tgtttggtga ttttgtactg
     2641 cagaccggaa gcagtggtct tcacgccgtc tttggcggca ttttccttca ggaaggcttc
     2701 gcctttttct ttgttggcct tcgcatccgc cttgtgtttt tctacggctt tagcctgctg
     2761 ctcctgcagg aatttcatca tcacttcctg ggcctgctct tcggtcattt tgatttcttt
     2821 gccgtcatac actgcctgca tggcatcggt aaagactttc aaatcgattt ccgcgccctg
     2881 ttccttcatt tgtttcaggg agcgtccgat gtccacgccc atcgcatagc ttgcctgctg
     2941 catcgtgctg ccgattgaag aggtgtcgcc ctgcgcggca gaagcggcgg caggttcgga
     3001 tgcaggtgcg ggggcggctt cttttttgcc gcaggcggaa agcgccaaag cggcggaaag
     3061 ggtcagtgcg ctgattttga aaatggtgtt catgatggat cttcgctgtc gataaagtcg
     3121 gaaaaacggg attatagccg agtttgaatg tttcaacaca caggatggca cataaagcgt
     3181 caatctgtgt gttgccctgt tttggaaggg attgaacctt ccaaaataag gtttgattct
     3241 accgccccga aggacagatg tccaagtggc ggggttcaac cgataaggaa attttaatca
     3301 aatagaatca agcctgttta aattttgtaa atgcggcatt tcagacggca tttcatgcct
     3361 tgccctccat gccgtgatgt tcgatggcaa aaccgcttcg gcggtaggcg gtaaagcgtt
     3421 cgcgcgcgtc ggcgagatct tccaggctgt tgccgacgat ttccaaaacg cgcgcgggca
     3481 agaccgaagc ggtgttccaa aaaccgtcgg acaggttcaa aacggccatg ccttcgggaa
     3541 ttcggggcag attgccgccg caggcaagca ggacggatgt gtcagacggc atggcttctt
     3601 ccgtttccca aatttcgtgc ggaataaaac tctcggcctc gtattgccaa agcattttgt
     3661 ccaattcctg aagccgcccg aacgagtcgg accacaccag tatcctgccg ccgtctcgga
     3721 tagcgcgcgc aatcaaccga caggtaaaaa gggggatttg gtcaacatgg gtataaaagg
     3781 taactttcgg catattgttt gagcatttga caggatgatg ccgtctgaaa agcttcagac
     3841 ggcattgtgg caaaattaaa gattccgcag atagttcatc agcagcggta cggggcggcc
     3901 ggtcgcacct ttttccgctc cggatttcca cgccgtaccc gcgatgtcaa ggtgcgccca
     3961 cgggtagcct tcggtgaaat aggacaggaa tgttgcggcg gtaatcgtgc ccgcgccggg
     4021 cgtgccgata ttggggatgt cggcaaagtt ggatttgagc tggtctttgt aggtttcaaa
     4081 gagcggcagt tgccacgctt tgtcgtccac gttgtaggaa gcggcaagca ggctgtcgat
     4141 caaatcctga ttattgccca tcacgccgct gacatcgtgc cccaaggcga cgatgcacgc
     4201 gccggtcagg gtggcgacat cgatgacggc tttgggtttg aactgctcgg cgtaagtgag
     4261 cgcgtcgcac aaaatcagac ggccttcggc atcggtgttc aacacttcaa tcgtcagccc
     4321 tttcatactt ttcacaacat cgcccggttt gtttgccgca ccggaaggca tgttttcaca
     4381 agtggcgacg atggcaatca gattgatcgg cagttgcagt ttgacggcgg cgcagaaggt
     4441 gctgatgacg gttgccgcgc cgcacatgtc aaacttcatt tcgtccatgt tcaggccggg
     4501 tttgagggaa atgccgccgg tgtcgaaggt aatgcctttg ccgaccaata ccacaggcgc
     4561 ggcttctttg tcggccgcac cgaaatagct cagttcaacc aaatatgggt cttcgacgct
     4621 gcctttggca accgaccaaa acgaacccat gttttctttg atgtagtctt tttcgatgat
     4681 tttggcgtgc gcgcccagtt tctcggcttc ggctttggcg gtacgcgcga ggaactcagg
     4741 cgtgcattcg ttcggtgcgg cattgcctaa gtcgcggcag aggctttgtc cgtaaacttg
     4801 tgcttcggca acgcgcaggg cttctttgac ggcggcttcg tgtgcgctgt ggaacacggc
     4861 ttgcgaaaat ttggcgggct tggcttcttt tttgtagcgg tcgaaacgat atgcagcgtt
     4921 gccgaacgcg atggcaaaag cttcggcaac ggctgctgct tgcgcctcat caaaggcgtg
     4981 aacgtccaca ttgaccgttt cttgattttg cgcccatttg gcggcttcgg cggcggcttt
     5041 gttcagcgcg gcgcggtcgg ttttttcgag gcggacgacg gcgactgcct gcaaaccgtt
     5101 gtccgtcggg attttggtgt cggaaaaatt ctgaccctct tccaaagcag agaagagcgc
     5161 atgtgcggcg gtgttgtgct cgggtgcttt gtcggcacag acaaataact gcgcgcctgc
     5221 ctgctgttcc tgcaagattt cggtttttgt gctaaattcc acgtttattc tcctgattga
     5281 gacggttgtc ggtagttttc agacggcctt tcgctcaaaa gaccgtctga agacggctgg
     5341 cacgattgta ccccattttt tgaagcaccg tctgaaacct tgcgcggaca atccgcctgc
     5401 gccgaaccgc ttaccgcccc cctgaccgcg attttatgat ttatcaaaga aacctcatca
     5461 aagaactctc ttttaccgcc gtcggcattt tcgtcgtcct cttggcggtg ttggtgtcca
     5521 cgcaggcgat caacctgctt ggccgcgcag ctgacgggcg tgtcgccatc gatgccgtgt
     5581 tggccttagt cggcttctgg gtcatcggta tgaccccgct tttgctggtg ttgaccgcat
     5641 tcatcagcac gctgaccgta ttgacccgct actggcgcga cagcgaaatg tcggtctggc
     5701 tatcctgcgg attggcgttg aaacagtgga tacgccccgt catgcagttt gccgtgccgt
     5761 ttgccatcct gattgccgtc atgcagcttt gggtgatacc gtgggcggaa ctgcgcaacc
     5821 gcgaatatgc cgaaattttg aagcagaagc aggaattgtc tttggtggaa gccggcgagt
     5881 tcaataactt gggcaagcgc aacggcaggg tttatttcgt cgaaaccttt gacaccgaat
     5941 ccggcatcat gaaaaacctg ttcctgcgcg aacaggacaa aaacggcggc gacaacatca
     6001 tcttcgccaa agaaggtaac ttctcgctga acgacaacaa acgcacgctc gaattgcgcc
     6061 acggctaccg ttacagcggc acgcccggac gcgccgacta caatcaggtt tccttccaaa
     6121 aactcaacct gattatcagc accacgccca aacttatcga ccccgtttcc caccgccgca
     6181 ccatttcgac cgcccaactg attggcagca gcaatccgca acatcaggca gaattgatgt
     6241 ggcgcatctc gctgaccgtc agcgtcctcc tgctctgcct actcgccgtg ccgctttcct
     6301 atttcaaccc gcgcagcgga catacctaca atatcttgat tgccatcggt ttgtttttaa
     6361 tttaccaaaa cgggctgacc ctgctttttg aagccgtgga agacggcaaa atccattttt
     6421 ggctcggact gctgcctatg cacatcatca tgttcgtcat cgcaatcgta cttctgcgcg
     6481 tccgcagtat gcccagccag cccttctggc aggcggttgg caaaagtctg acattgaaag
     6541 gcggaaaatg aacctgattt cacgttacat catccgccaa atggcggtta tggcggttta
     6601 cgcgctcctt gccttcctcg ctttgtacag cttttttgaa atcctgtacg aaaccggcaa
     6661 cctcggcaaa ggcagttacg gcatatggga aatgctgggc tacaccgccc tcaaaatgcc
     6721 cgcccgcgcc tacgaactca tgcccctcgc cgtcctcatc ggcggactgg cctctctcag
     6781 ccagcttgcc gccggcagcg aactggccgt catcaaagcc agcggcatga gcaccaaaaa
     6841 gctgctgttg attctgtctc agttcggttt tatttttgct attgccgccg tcgcgctcgg
     6901 cgaatgggtt gcgcccacgc tgagccaaaa agccgaaaac atcaaagccg ccgccattaa
     6961 cggcaaaatc agcaccggcc ataccggcct ttggctgaaa gaaaaaaaca gcattatcaa
     7021 tgtgcgcgga atgttgcccg accatacgct tttgggcatc aaaatttggg cgcgcaacga
     7081 taaaaacgaa ttggcagagg cagtggaagc cgattccgcc gttttgaaca gcgacggcag
     7141 ctggcagttg aaaaacatcc gccgcagcat catgggtaca gacaaaatcg aaacatccgc
     7201 cgccgccgaa gaaacttggc cgattgccgt cagacgcaac ctgatggacg tattgctcgt
     7261 caagcccgac caaatgtccg tcggcgagct gaccacctac atccgccacc tccaaaacaa
     7321 cagccaaaac acccaaatct acgccatcgc atggtggcgt aaactcgttt accccgtcgc
     7381 cgcatgggtc atggcgctcg ttgccttcgc ctttacgccg caaaccacgc gccacggcaa
     7441 tatgggctta aaactcttcg gcggcatctg tctcggattg ctgttccacc ttgccggcag
     7501 gctcttcggg tttaccagcc aactctacgg caccccaccc ttcctcgccg gcgcactgcc
     7561 taccatagcc ttcgccttgc tcgctgtttg gctgatacgc aaacaggaaa aacgttgaac
     7621 aaatgccgtc cgaacctctc ttcagacggc atttgttttc attgacacat tcccgcagac
     7681 atatagccgt tccctattat agtggattaa atttaaaccg gtacggcgtt gcctcgcctt
     7741 gccgtactat ttgtactgtc tgcggcttcg ccgccttgtc ctgatttaaa tttaatccac
     7801 tatacattac ctgtcataac agttccattt ttgttaaaac tagtctatga tagcggtaca
     7861 aatgttgttt acaatattta acgcagatca tttgcaaccc gacaaaagaa aaacggaaaa
     7921 aggaacaaag agatgttaga agcctatcgt aaagccgccg ccgagcgcgc cgccctcggc
     7981 attcccgccc tccctttgaa tgcgcagcaa accgccgatt tggttgagct gctgaaaaac
     8041 ccgcccgcag gcgaaggcga gttcttggtc gagctgcttg cccaccgtgt tccgcccggt
     8101 gtggacgatg ccgccaaagt caaagcctca ttcctggctg ccgttgccga aggcagcgca
     8161 tccagcccgc tggtttcccc caaatatgcg accgaactct taggtacgat gctcggcggt
     8221 tacaatatcc acgccttaat cgaactcttg gacgacgaca aactcgcgcc cattgccgcc
     8281 aaaggcttga aacatacgct tctgatgttc gattccttcc acgacgttca ggaaaaagcc
     8341 gaaaaaggca acaaatacgc gcaggaagtt ttgcaatctt gggcagatgc cgaatggttc
     8401 gcctcccgtg ccaaagttcc cgaaaaaatc accgttaccg tcttcaaagt tgacggcgaa
     8461 accaatacag acgacctctc tcccgcgccc gacgcgtgga gtcgtcccga tattccgctg
     8521 cacgcgctgg ccatgctgaa aaacccgcgc gacggcatca cgcccgacaa accgggcgaa
     8581 gtcggtccga ttaaattgtt ggaagaactc aaagccaaag gccatccggt tgcctacgtc
     8641 ggtgacgtgg tcggtaccgg ttcttcacgc aaatccgcga ccaactccgt catttggcat
     8701 accggcgaag acatcccgtt cgtgccgaac aaacgcttcg gcggcgtgtg cttgggcggt
     8761 aaaatcgcgc cgattttctt caacactcaa gaagactccg gcgcgctgcc gattgaagtc
     8821 gatgtttccg cgctgaaaat gggcgatgtc gtcaatatcc ttccttatga aggtaaaatc
     8881 gtgaaaaacg gcgagactgt tgccgagttt gaattgaaat cacaagtatt gctggacgaa
     8941 gtgcaagccg gcggccgtat caacctgatt atcggtcgcg gtctgaccgc caaagcgcgc
     9001 gaagccctga aactgcctgc ctctactgca ttccgcctgc cgcaagcgcc tgccgaaagc
     9061 aaagtcggtt tcaccttggc gcaaaaaatg gtcggtcgcg cctgcggtct gcccgaagga
     9121 caaggcgtgc gtccgggtac ttactgcgaa ccgcgcatga cgacggtcgg ctcgcaagat
     9181 acgaccgggc cgatgacccg cgacgagttg aaagacttgg cttgtttggg cttctccgcc
     9241 gatatggtga tgcagtcttt ctgccacacc gctgcctatc cgaaacctgt cgatgtaaaa
     9301 acccataaag aactgcccgc ctttatttct acccgtggcg gcgtgtccct gcgtccgggc
     9361 gacggcgtca tccactcgtg gctcaaccgc ctgctgttgc ctgatacagt cggtacaggc
     9421 ggcgacagcc acacccgttt ccccatcggt atttccttcc ccgcaggttc aggcttggtc
     9481 gcttttgccg ccgcaacggg cgtaataccg ctcgatatgc ccgagtccgt attggtacgt
     9541 ttcagcggca agctgcaacc gggcgtaacc ctgcgcgatt tggtgaacgc catcccgctt
     9601 tacgcgatta aacaaggttt gctgaccgtt gccaaagccg gtaagaaaaa catcttctcc
     9661 ggccgcatcc tcgaaatcga aggcctgccc gatttgaaag tggaacaagc ctttgaattg
     9721 accgacgcat ccgccgaacg ctccgccgcc ggctgtaccg tgaagctcaa taaagagccg
     9781 attatcgagt acatgaaatc caacgtcgtg ttgatgaaaa atatgattgc caacggctat
     9841 caagatccgc gcgctttgga acgccgcatc aaagctatgg aaaaatggct ggcaaatccc
     9901 gagttgctcg aagcggataa agatgccgaa tacgccgccg tgattgaaat caacatggac
     9961 gacatcaaag agccgattat cgcctgcccg aacgacccgg acgacgtatg ctttatgtcc
    10021 gaacgctccg gcaccaaaat cgacgaagtg ttcatcggtt cttgtatgac caacatcggc
    10081 cacttccgcg ccgcctccaa acttttggaa ggcaagagcg acatccccgt ccgcctgtgg
    10141 gtagcgccgc cgaccaaaat ggacgcgaaa gagttgtccg acgaaggtca ctacggcgta
    10201 ctcggccgcg ccggcgcgcg tatggaaatg ccgggctgtt cgttgtgtat gggtaaccaa
    10261 gcccaagtac gcgaaggtgc aactgtcatg tccacttcga cccgcaactt cccgaaccgc
    10321 ttgggtaaaa acaccttcgt ttacctcggc tcggcagagt tggcggcgat ttgctccaaa
    10381 ctgggtaaaa tcccgaccgt tgaagaatat caagccaata tcggcatcat caacgaacag
    10441 ggcgataaaa tctaccgcta tatgaacttc aacgaaatcg acagctacaa cgaagtagcc
    10501 gagaccgtga atgtttgatc cccgtcatcc gtatggagta agggattgac cgcaatgccg
    10561 tctgaacaac cttcagacgg cattgcaaca ttccgctaac ccttctttcc gcaaacgctg
    10621 caaatacggc gttcacgccc ccacataaag gaaacgacag tgaacctgaa aaaccgccat
    10681 tttctgaaac ttttggactt cacgccggaa gaaatcacca cctacctcga ccttgccgcc
    10741 gagttgaaag acgccaaaaa ggcagggcgc gagattcagc ggatgaaagg gaaaaacatc
    10801 gccctgattt ttgaaaaaac atccacgcgc acacgctgtg cgtttgaagt cgccgcacgc
    10861 gaccaaggcg cggatgcaac ctatctggaa ccgtccgcca gccaaatcgg gcacaaagaa
    10921 agcatcaaag acacggcgcg cgtcttaggc agaatgtacg atgccatcga atatcgcggc
    10981 ttcgctcagg aaactgtcga agaattggca aaatatgcgg gcgtacccgt gttcaacggg
    11041 ctgaccaacg agttccatcc cacacaaatg cttgccgacg cactgactat gcgcgaacac
    11101 agcggcaaac ctttgaacca aaccgcgttt gcctacgtcg gcgacgcgcg ttacaacatg
    11161 ggcaattccc tgctgatttt aggggcaaaa ttggggatgg acgtgcgtat cggcgcacct
    11221 caaagcctgt ggccgtctga aggcattatc gccgccgcac acgccgccgc caaaaaaacc
    11281 ggtgcaaaaa ttaccctgac cgaaaacgcg catgaagccg tcaaaggtgt cggtttcatt
    11341 catactgacg tatgggtcag catgggcgag ccgaaagaag tctggcagga acgcatcgat
    11401 ttgctgaaag attaccgcgt tacgcccgaa ctgatggcgg catcgggcaa tccgcaagtc
    11461 aaattcatgc actgcctgcc cgccttccac aaccgcgaaa ccaaagtcgg cgaatggatt
    11521 tacgaaacct tcgggctgaa cggtgtggaa gttacagaag aagtattcga aagtccggcc
    11581 ggcatcgtgt tcgatcaggc ggaaaaccgt atgcacacga ttaaagcggt aatggtcgcg
    11641 gctctgggcg actgacagaa ctgtgcctgt ttaaattcat ccgcaacaca gataccgtcc
    11701 gaacacgatg ttcagacggt atccatataa caaactgcct acacgatgtg taggcagtcc
    11761 cgtttgaaaa caatcagttt ttgtcttggt caaccaattt gttggccgta atccaaggca
    11821 tcatggcacg cagtcgtgcg ccgacttttt caacttggtg gtcggcattc aaacggcggc
    11881 gggcagtcat agacgcgtag ttgacattac cctcttggat aaacattttt gcgtattcgc
    11941 cggtttggat gcgtttcagg gcattgcgca tggcttcttt gctggaagca ttgaccactt
    12001 cagggccggt aacgtattcg ccgtactccg cattgttgga aatggagtag ttcatattgg
    12061 caataccgcc ttcgaaaatc aggtcaacga tcagtttcat ttcgtgcagg cattcgaagt
    12121 aagccatttc aggcgcgtaa ccggcttcgg tcagggtttc aaaacctgct ttgatcaact
    12181 cggccacgcc accgcacaat acggcttgtt cgccgaacag atcggtttcg gtttcttcgc
    12241 ggaaagtggt ttcaatcaca ccgcctttgg tgccgccgtt ggcagccgca taagacaggg
    12301 cgatgtcttt ggccttgccg gaattgtctt ggtaaacggc aatcagagaa ggcacgccgc
    12361 cgccgcgttt gtattcactg cgtacggtat gacccggacc tttgggggca accataatca
    12421 cgtccaagtc ggcacgcgga acgatttggt tgtagtgcac gttgaagccg tgtgcaaatg
    12481 ccagcgtcgc gccttctttc aaattggctg caacttcggc gtgatagacg gcaggcatgg
    12541 tttcgtcagg cagcagcagc ataacgacat cggcttcttt ggtcgcttca gcaacggttt
    12601 taacaacatg gccggctgct tcggcttttt tccaagaaga gccgtggcgc agaccgatca
    12661 ccacgtttac acccgaatct ttcaggttgg cagcatgggc atgaccttgc gaaccgtaac
    12721 cgatgatggc aacggttttg cctttgatca gggacagatc ggcatcttta tcgtaataga
    12781 cttgcatttg atttccttta aggtaaatgg ttgtcgaagc cttaaaatgt tgagcggctt
    12841 cggacgggtt aaacagagtg tgccgcttaa acggcaactt cattcatcaa tacgatttcc
    12901 aacgcttcgg ttttgccgtc gacggactgg acgaaggctt ggaaatgcgc gctggcatta
    12961 tgttcgtcaa tagctgcttg ggatttccaa ttttccacga aaacaaaacg gttcggtttg
    13021 ccgatttcct gatggagatt gtagctgatg ttgccctctt ccgcacggct ggctttgacc
    13081 agttctttaa actgtgctgc cagtgtttcc gtgtattccg gtttgacggt aaccagtgcg
    13141 acaattttaa tgttcgacat aaatctctcc tgccgttcgt ttttcagacg gcattcaaat
    13201 accatgccgt ctgaaaggtt acggcgttaa attttcaaaa tacgctcacc gcgaccgatg
    13261 ccggccgcac ctgtacgtac ggtttccaaa atttgggcgc gtccgaccgt ttccaaaaag
    13321 gaatccagct tgtcggtcga gccggtaatt tcaatcgtat agctgcggtc ggttacgtcg
    13381 atgatgctgc cccggtagat ttcggtcaag cgtaaaaatt cgtcgcggtc tttgccggcg
    13441 gcacggactt ttaccaacat cagttcgcgt tcgacaaaac ggctttcgtt caaatcgacc
    13501 actttaatca cttcaatcaa tttattgagt tgcttggtga tttgttcgat aacctgctcg
    13561 tcgccgtggg taacgatggt catccgtgac agggttttgt cttcggtcgg cgcaaccgcc
    13621 aaagaatcga tattgtaatc gcgtgcagag aacaaaccga ccacgcggct catcgcacct
    13681 gattcgtttt cgatcagaac agataagata tgtcgcattt gcctctcctt acgcctttcc
    13741 gtccgtacgc atatgcggcg gaagtaccat ttcgtccaaa cctttgccgt tgccgaccat
    13801 aggcatcaca ttctgtttct ggtcggtcag gaagtcgata aacaccagcc tgtctttttg
    13861 gttcaatgct tccaacaagg caccttccac atcagatttc ttgtccacgc ggatgccgat
    13921 atggccgtat gcctcggcaa gtttgacgaa atcgggcaaa gaatcgaaat aggtttccga
    13981 ctctcgaccg ccgtaatata tttcctgcca ctggcgtacc atacccagat aaccgttgtt
    14041 cagcgtaatg acgttaaccg gaatccgata ttggaaacag gtggacaact cttggatgtt
    14101 catctggatc gagccgtcgc cggtaataca gaatacgtct tgatccgggg cggcaagttt
    14161 tgcaccaatc gcataaggca gacccacgcc catcgtaccc aaaccgccgg agttgagcca
    14221 ttggcgcgga cgttcgaaag gataatattg agccgcaaac atttgatgct gccctacatc
    14281 cgatgtgatg attgccgaat tgccggtaat ctcggcaagc ttctgaatca catattgggg
    14341 cttgataatt tcgctgccgt tgtcaaacca caagcaatcg cgggaacgcc attcctctat
    14401 ggttttccac catttgccca aagcatcttc agacggcacg gactcttgtt tttgccacag
    14461 cgcaaccatc tcggacaaaa tgtttttcac gtcgccgaca atcggaatgt ccgccttcac
    14521 gcgtttggcg atgctggaag gatcgacatc gatatggata accttcttcg ccttctcgaa
    14581 aaatttggac ggtacggaaa ccacacggtc gtcaaaacgc gcacctacgg caagaacgac
    14641 atccgcattc tgcatggcaa ggtttgcctc gtaagtaccg tgcataccga gcataccgag
    14701 gaattggcgg tcgccggaag gataagcgcc caagcccatc agcgtacccg tgcacggagc
    14761 acccgtcatt cggacaaatc gggtcagctc ttcagaagca ttacccaaca ccacgccgcc
    14821 gccaaaatag acgaccgggc gtttggcaga tgccaacatc tgcacggcct ttttaatctg
    14881 accgatatgc ccttgaacaa ccggttgata cgaacggata aaaatgtctt cctgaggata
    14941 gctgaatttc gccatcgcct gcgtaacatc tttcgggaca tcaagcacca cgggccccgg
    15001 tcggccgctt gcggcaattt ggaacgcctt tttaatggtt tccaccaact cattgatgtc
    15061 cgtaaccaag aaattgtgtt tgacgcacgg acgggtaata cccaccgtat caacttcttg
    15121 gaacgcatcc gtaccaatca gggaattgcc tacctgtccg ctgatgacca ccatcggaat
    15181 cgaatccgta taggcagtag caataccggt cagcgcattg gtaacgcccg ggccggatgt
    15241 aaccaatgcc acgcccacct taccgctgac gcgcgcatac gcatctgccg cgtgtactgc
    15301 cgcctgctca tggcgggtaa gaatgtgttt gaatttattg agttggaaca gggcatcgta
    15361 gatttcgata accgcaccgc cgggataacc gaaaacgtac tcgacgcctt cggctttgag
    15421 actctgcact atgatttgcg cgcctgataa ctgcataacg acctctttta tacggtttca
    15481 aaccaatagg gacaaaccgc tttgccacag cacctgtaat gcaattccac caagcagcga
    15541 tttagggtac gcgcattggg ggaacacggc aacagacaga ttatccaatc aattggaaag
    15601 gaacacagag tttgtgaaaa agagtaaaaa cgataacgca aaccgacagt tcaatcaaga
    15661 aaaatctttc atcttttaat attttttgaa agcggagaaa tttttttgaa agcggagaaa
    15721 ttattgattg attttaaaag aataaaatca ggagtacctt ttttgaaaga tggaaattgt
    15781 tgacagtttg catgggggca gatgtgaaag tatcaagaat tttaaaattt acagggtttc
    15841 actcgggata ttgattgaac ttgattttat ttttgatata tcaaaaatat tcccaaccat
    15901 acttcctgaa aatggctcat tgcaccggac ggcattgaca gaaccaagag ggctaacaac
    15961 gacttaaata tattgattgt atagtggatt aaatttaaat caggacaaaa cgacgaagcc
    16021 gcagacagta caga
//
LOCUS       DAXFWM010000057        15890 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000057 DAXFWM010000000
VERSION     DAXFWM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15890
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.057"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..428
                     /locus_tag="ACFI8B_001638"
                     /pseudo
     CDS             1..428
                     /locus_tag="ACFI8B_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            483..695
                     /locus_tag="ACFI8B_001639"
     CDS             483..695
                     /locus_tag="ACFI8B_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC3834815.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            880..1404
                     /locus_tag="ACFI8B_001640"
     CDS             880..1404
                     /locus_tag="ACFI8B_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC3834816.1"
                     /translation="MTDRQIDNLIKPATIADTDPASNRVRAQHGGITTDWLPYIVPFA
                     GGVSVWRIPSVGEACTILSPAGEPENGVVLCCQASDRYPAPSADPAETVVRFPDGTHI
                     RYNHNSGAMELKAVTSLTIDTPQTTITGHLTVNQTTTAQGLLTYQNGMNGQGGSLSEH
                     THPDDSGGTTEKPQ"
     gene            1401..1727
                     /locus_tag="ACFI8B_001641"
     CDS             1401..1727
                     /locus_tag="ACFI8B_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFC3834817.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1988..2458
                     /locus_tag="ACFI8B_001642"
                     /pseudo
     CDS             1988..2458
                     /locus_tag="ACFI8B_001642"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            2634..3197
                     /locus_tag="ACFI8B_001643"
     CDS             2634..3197
                     /locus_tag="ACFI8B_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC3834818.1"
                     /translation="MNSTVPANNSPLQHALAKLTERETAAVSRQLDPARCDPGFLPFH
                     AFARSIGTEEGWDFAETDEARRNLIAGFAEIHARKGTPYAIRALFPILRLGEIQIIER
                     DGEFKWDGSVLFDGSRTFGRREGDWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCEL
                     TALDYRNHPHRWNGKIRFNGEYGFGTT"
     gene            3237..5421
                     /locus_tag="ACFI8B_001644"
                     /pseudo
     CDS             3237..5421
                     /locus_tag="ACFI8B_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5533..5862
                     /locus_tag="ACFI8B_001645"
     CDS             5533..5862
                     /locus_tag="ACFI8B_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834819.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5878..6189)
                     /locus_tag="ACFI8B_001646"
     CDS             complement(5878..6189)
                     /locus_tag="ACFI8B_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFC3834820.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(6197..6476)
                     /locus_tag="ACFI8B_001647"
                     /pseudo
     CDS             complement(6197..6476)
                     /locus_tag="ACFI8B_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6561..7174
                     /locus_tag="ACFI8B_001648"
                     /pseudo
     CDS             6561..7174
                     /locus_tag="ACFI8B_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7153..7317
                     /locus_tag="ACFI8B_001649"
                     /pseudo
     CDS             7153..7317
                     /locus_tag="ACFI8B_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            7418..7552
                     /locus_tag="ACFI8B_001650"
                     /pseudo
     CDS             7418..7552
                     /locus_tag="ACFI8B_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            complement(7636..8019)
                     /locus_tag="ACFI8B_001651"
     CDS             complement(7636..8019)
                     /locus_tag="ACFI8B_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC3834821.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(8124..8279)
                     /locus_tag="ACFI8B_001652"
     CDS             complement(8124..8279)
                     /locus_tag="ACFI8B_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834822.1"
                     /translation="MAETVMPEYRNWQNGLDAKCADGDEYGVAQSDCRTREINAKTKE
                     IQGYLID"
     gene            complement(8309..8761)
                     /locus_tag="ACFI8B_001653"
     CDS             complement(8309..8761)
                     /locus_tag="ACFI8B_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC3834823.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            complement(9539..11185)
                     /gene="pgi"
                     /locus_tag="ACFI8B_001654"
     CDS             complement(9539..11185)
                     /gene="pgi"
                     /locus_tag="ACFI8B_001654"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC3834824.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            complement(11411..12259)
                     /gene="hexR"
                     /locus_tag="ACFI8B_001655"
     CDS             complement(11411..12259)
                     /gene="hexR"
                     /locus_tag="ACFI8B_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC3834825.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(12308..13294)
                     /locus_tag="ACFI8B_001656"
     CDS             complement(12308..13294)
                     /locus_tag="ACFI8B_001656"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC3834826.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            complement(13275..13970)
                     /gene="pgl"
                     /locus_tag="ACFI8B_001657"
     CDS             complement(13275..13970)
                     /gene="pgl"
                     /locus_tag="ACFI8B_001657"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC3834827.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            complement(14121..15566)
                     /gene="zwf"
                     /locus_tag="ACFI8B_001658"
     CDS             complement(14121..15566)
                     /gene="zwf"
                     /locus_tag="ACFI8B_001658"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC3834828.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
BASE COUNT         3773 a         4761 c         4079 g         3277 t
ORIGIN      
        1 ggacggcgta aaggcggacg cggtcgcggc gcggtaggaa aagcggttgt cttcggcatt
       61 ccgaaacgca acgggcgggc ctataccgtt gccgaggacg atgccgagcc tgaaacgttg
      121 ctccctgccg tcaaaaaaaa atcatgccgg acggtattgt ttatgccgat agcccgggca
      181 gccgcggcaa gtcggacgcg ggcggtttta cccgttgccg catcaaccgt tccaaggaat
      241 ttgcagaccg tcggaaccac attaacggca ttgggaactt ttggaatcag gcaaaacgcg
      301 ccttgcggaa atacaacgga atcgatcgta aacctttccc gccgttcttg agggaatgcg
      361 aatttcgatt taacttcggc acaccgtccc ggcagctaaa aatcctgcgg gatcggtgtg
      421 gaatttaggg ataatctagt acagccacaa aaaatatgcg aaacaaaata aaccgcaacg
      481 acatggaact cggctacacg ccttacaacc ttcgcaccct gcgcaaccgc tgcaaactga
      541 cacaggccga gttggcgcaa atcgtcgggg tgaagcacta catacaggta ggccgctggg
      601 aagccgaacc cgataccgaa acgcggcggg ccgatatgcc gctggaaaaa tggcggcagt
      661 tcctcgattg gatagaaaaa acaaacgccg tctgaaacct tcagacggca ttttttaccc
      721 acccccaaac ctaaaaacca caaccaagcc ctacaccgct tgcccccacc tcgccgccgc
      781 cccaaaataa cctcactctg ttggaagcct gccggaaacg gcaaaagccg ccccgttccg
      841 ccattacggg gcggtttccc acatcaagac gagacagaaa tgaccgaccg ccaaatagac
      901 aacctcatca aacccgccac catcgccgac accgaccccg cgtccaaccg cgtccgcgca
      961 caacacggcg gcattaccac cgactggctg ccctatatcg tcccctttgc aggcggcgtg
     1021 tcagtatggc gcatcccaag tgtcggcgaa gcctgcacca tcttatcccc tgccggcgaa
     1081 cccgagaacg gcgtagtttt atgctgccaa gcctccgacc gataccccgc cccgtccgcc
     1141 gaccccgccg aaaccgtcgt ccgatttccc gacggcacgc acatccgcta caaccacaac
     1201 agcggcgcga tggaattaaa agccgttaca agcctgacca tcgacacccc ccaaaccacc
     1261 ataaccggac acctgaccgt caaccaaacc accaccgccc aagggctgct gacctaccaa
     1321 aacggcatga acggccaagg cggcagcctg tccgagcaca cccaccccga cgactccggc
     1381 ggtacaacgg aaaaacccca atgaccgacg cagaaaacgg acgcgggcaa gacaccctcg
     1441 cccacatcgc ccaatccatc cgcaacatcc tgttcacaag aataggaacg cggctgatgc
     1501 gcgaagaata cggcagtttt attcccgact tgatcgatat gcccgccggt cacgccgcca
     1561 tcgccctcat ccaccaagcc gccgttaccg ccctcgcaag atggaaaccc cgcataaccg
     1621 tccgccgcat acaggcagac accgccgacc tcgccgccgg caaaatcaaa ttaaccctag
     1681 acgtaaccct tgccgacggc ggcgaacgaa cctaccgcat caaataataa aaacaacagg
     1741 ataaacaatg ggaaacagcc gattaagcca actccccgcg cccgccgcca tcgaagaaac
     1801 cgactttgag ggcatcttcg cgcgcaaaaa agccgccctc accgccctat gtcccgaaag
     1861 catccgcgaa accgtcgccc aaaccctcga attagaatca gaacccctaa ccatcgacct
     1921 gcaacagcaa gcctatcaag agctgctcgt ccgcaaccgc atcaacgaag ccgtcaaagc
     1981 caacctcttg gcatacgcac aaggcagcga cctcgaccac atcgccgccc aatacggact
     2041 ttcacgcaaa accatccgcg ccgccgaccc cgacgcgaac ccgccggttg ccgccgaata
     2101 cgaaaccgac gacgcattcc gcgcccgcgt ccaagcccat cccgaaaaat acgccgccgg
     2161 gccgcgcacc gcatacgaag cccacgccat cgacgcacac ccccaaatca cacacgcccg
     2221 cgccgtgcgc cgcgccgccg gcacggtgga ggtttacatc aaaacccaaa gcggcacgcc
     2281 cgacgaaacc attttgaccg ccgcgcgcga atacctgccc gccgaaacac gccgccccct
     2341 ttgcgacaac gtgcaagtaa ccgccgccca acccaaagac gcggcagtag agtattccgc
     2401 cgaataccac cccgccgcca atattcaagc cgaacggcag accgcctgcg aagccttgga
     2461 taacctatgg cggcaaaacg cccacatcgg cgcatccgtc gccctgtcta aaatcatcgg
     2521 cgcgttagac acccccggcg tgaaaaaaaa tcacactgca cagccccgct gccgacatcg
     2581 aatgcggcaa cggcgaatac atccgaatca cgtccacgct cgagcgcgaa taaatgaaca
     2641 gcaccgtccc cgcgaacaac agccccctgc aacacgcact ggcaaagctg acagaacgcg
     2701 aaactgccgc cgtctcccgc caactcgacc ccgcccgatg cgaccccgga tttttaccct
     2761 ttcacgcctt cgcaagaagc atcggcacgg aagagggctg ggactttgcc gaaaccgacg
     2821 aagcccgccg caacctcatc gcaggctttg ccgaaatcca cgcccgaaaa ggcacgccgt
     2881 acgccatccg cgccctcttc cccatcttgc ggctgggcga aatccaaatt atcgaacgcg
     2941 acggcgagtt caagtgggac ggctcggtct tgttcgacgg cagccgcaca ttcggcaggc
     3001 gcgagggtga ctgggcggaa taccgcattg tcttaacgcg ccccgtcagc atccgccaaa
     3061 ccgcccgcat ccgcgccatg ttggcggaaa tcgccccctt gcggtgcgaa cttaccgcgc
     3121 tcgactaccg caaccatccc caccgctgga acggcaaaat ccgctttaac ggcgaatacg
     3181 gtttcggcac gacataacgc gcccccgaaa atcaacaaac aaaaggaagc cccaaaatgg
     3241 caaacgcaac cgaacaaaac caattcgacc aagccgtccg cctcatcgaa tccggcgaca
     3301 gcgtcgtcgg cccaggcgcg cccgtcaacc aaccactgca agccctcgcc aaccgcaccc
     3361 ttttgctcaa aaaccaaacc gaagcccttc agacggcatc cgacaccaaa gccgcaccat
     3421 cgcactgggc gcgcccgggc aaatcaccgc aacaagccaa aacaccgtcc caaaaaacgg
     3481 ccacacccac gccatcgaca ccgcacgcac cgaccgcgcc ggcatcgtcc gattagacaa
     3541 tgccattagc gaagccgaag acaccgccgc cacccccaaa gccgtcaaaa ccgcgctcga
     3601 ccaagcccgc gccgccgccg cgaccgccga cctcaaagtc tccctctccg acaaccaaac
     3661 cgttaccggc caaaaaacct ttaccgccga aacacaattc caaagcggca tccggctgtc
     3721 cgccaacccg acgcactgaa acggcggcta caaagcctat atcggcgcgg ataacgaaaa
     3781 cgcccacatc gtcttcggcg acgacaccct gcgcctgcac ggcgcaaata accgcatttc
     3841 ctacaacaac cacgacatct tccacaaagc caacaaaccg cgtttcaacg aagacatcga
     3901 aggcaaaccg aacacgctgt ccggctacgg catcggcaac ttcaaagtcg aaacattccg
     3961 gggcgatttg aacagcctca aaaccgacgg cgtctattcc ctgccgacgg cggtcggcag
     4021 ctcgaacctg cccgttgaaa acaccgcctg ccatatccaa gtcatcgccg gcacgcaacc
     4081 cggctggtgc aggcagttgg gctatcccgc ctacacgtcc gacgtgtacg aacgctacca
     4141 gaccagcagc gcaaacgacg actggagcgc gtggaaaaaa ctcaattcgg aaggcatccc
     4201 cgccggcgcg atcgtgtcct ttcccaaagc cgtccgaaac cccgcaggct atctcaaagc
     4261 cgacggcacg atctttgcac aaaacacctt ctcgaccttt accgcgccct gggcaacaca
     4321 aacaaactgc ccgacctgac ccgtaccgac atcggcatca ccgcgtggtt tccgtccgac
     4381 caaatccaga cgggctggct ggcgtttgac gacatccgag agcgcgtaac cgaatcggct
     4441 tatcccgaac tttaccgcct gctgaccgaa aaatacggac gcatccaaaa cgtcccgcag
     4501 gcggaagacc gctttatccg caacgcgggc aacggcttgg cggtcggaac gaagcaggaa
     4561 gacgaaatca aacggcacgt ccacaaagta ttttcacact gggcaaacca cccagacgcc
     4621 gccgccgtcg gttacgaaga ccgcaatgaa aggcaaagaa gcgcgctcgt atcgacttgg
     4681 acggacggcg acttaaacga caacggcttt ttaaccccgc gattggacag caaaatggca
     4741 acgggcggcg cagaaaaccg ccccaaagcc ctggttttaa aactgtgcat caaagccgcc
     4801 gacaccttgg gcgaagccgt gttccggata aagtcccacg gcgaaaccgc caacgccggc
     4861 gcactggacg tgtcaaggct ggcgcaaggt ttgcaggaaa aagccgaccg cgaccacacc
     4921 cacaccaccg cccaaatcca agggctggac gaaaaaatca gcgccgccgt tgccgcgcaa
     4981 ttcacacgcc aaaccatcgg cggcgtggat attgtcagat tccccgacgg cacaatgata
     5041 cagaccggca gttacaggtt tgcaagaggc ggcagcccca taggaaacga agttgtcttc
     5101 cccatcgcct ttgccgacgg caacgtcaaa tgcttcgtat ccgaacgcca ttcgggacgc
     5161 gtcaacagcg aaaggcaaca caactggctg tttatccgcg caaaaaacca cgccgccgcc
     5221 attattacca actggtacga aagcagttgc gactggatgg ccatcggcaa atccgcctcg
     5281 ggaaacgccg ccagccccac cccgatagtc cccgaaatac ctgaaatcga tgaagaaccg
     5341 caaagagaga gtggaagatc atcaaccgga ctccgaaacc cccgccgcca ccgaggcctc
     5401 gacttccccg tggggtcgta agactagcgg gcggcagggc aggctgtaga gacgggctgt
     5461 agagacgggc ttcagcccac cgatccaagc aatccgaccg aaaccggccg cgccgccaat
     5521 cctgcgaaac ctatgccccg ccaatcctgc cactcttcgc cattcccgcg aaagcgggaa
     5581 tccggaaccc aaacgcggca ggaatctatc ggaaagaata acccgaccgc cgtcattccc
     5641 gcgcaggctg gaatccagac ccacaacgtt aaggcggttt atcagaaaaa accgaaaccg
     5701 aacgccctag atttccgcct gcgcgggaat gacgaagagt tggggagtga cgaaaggcgg
     5761 gaacaaccgc gcaaaaagcc gccgactcct tcagacggca tcggcaataa aaaccgcacg
     5821 gccgaaaccg cgcgggaaag gatagccggg cgcgcccgat aagcagcggc cgcagggtta
     5881 agtccttgca cctgttttca tcgaagacaa aggcggttcg ggaaataagg gcgcaatatc
     5941 aaaagttttg cgttgcctga tttgccacaa atcataaccg gcctgcatat tcagccaaaa
     6001 atcaggcgaa gtgttgagca gttggcttaa acgaaccgcc atctcgggcg taatcgaaga
     6061 ttgggcgttg acaatgcgcg ataaggcaac ccggctgaca cccaggcgtt tcgctatatc
     6121 ggtaatgctt gcgccgttaa tatactcttt taaaacaagg cccgggtggg caggattgtg
     6181 cattttcata ttattcctag tgataatcct gataatcgac aacttcgaca ttgccgtcgt
     6241 tgaaacgaaa agtcaaacgc caatttccgt tgactttgaa ccgcccaatg tcccgacaaa
     6301 ctgcccttga ggggatgaag attccaactc ggcacagcca tatccgaagg cgtttgcgcg
     6361 gcattcagcg cggttagaag cagattcagt ttgacggaat gcccggcttg gatgcccgat
     6421 agcgagcctg ttttaaaaaa gcgttcaagc cctttgtgtt tgaaagaaac aatcatcatc
     6481 ccaccccgtc tgtatcgcca aaggatacaa aacttttatt tgattgcaaa gcgacgatat
     6541 acaaaaaagg aaaccccgaa atgaccatct atttcaaaaa cggcttttac gacgacacat
     6601 tgggcagcgt ccccgaaggc gcggttgccg tcggcggcga agaatacgcc gccctcttcg
     6661 caggacaggc gcagggcggg caaattgccg ccgattccga cggccgcccc gttttaaccc
     6721 cgccgcgccc gtccgagtac cacgaatggg acggcaaaaa atggaaaatc agcaaagccg
     6781 ccgcccgttt cgccaactcc cgtttcgcca aacaaaaaac cgccttggca ttcagcctcg
     6841 cggaaaaggc ggacgaactc aaaacagcct cttggcgggc tatccccaag tggaaatcga
     6901 cagcttttac aggcaggaaa aagaaaccct cgcgtggcag gcggacaacg acgccccgac
     6961 cccgatgctg gcgcaaatcg ccgccgcaag gggcatagaa ttggacgttt tgattggaaa
     7021 ggttgtcgaa aaacccgccc gcctggccgt tgccgtcgac gcgattatcg gaaagcggca
     7081 gcaacttgaa gatagattaa ataccatcga aaccgcgcca gcattagatg cgttagaaag
     7141 ggaaatcaaa gaatgggcgc taaacgtcgg ctgagagaat atatttatca tctactagtg
     7201 gccatagacc aactgttcaa cgctataacc ggcggcgcgg cagacgaaac cctctcaagt
     7261 cgcacctatc gcggcgcgcg gctcgcccaa caacccaaaa cccgctggaa gattttatag
     7321 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa
     7381 ggcgaggtaa cgccgtaccg gtttttgtta atccactata tataccctga tcaacggcgt
     7441 atttttcgac cgccaccact gccggcaggc gtatatcagc gaactgaaag gcaggcagca
     7501 cgacgcgcgg ttcaaccaaa gccgcgccgc caatgaaagg gatacgcggt aaaattgccg
     7561 ccgcgccgca ttcagacggc atcggcaata aaaaagcccg aaaaaatcgg gctgaaagtt
     7621 aaggctgcat cgcctttaag gcggctttcg ccaaaaaacc gctcgggttt catgacgggc
     7681 ggcagtgcag gcatcgacac ggttgaggat atgttgcggc caactgacgt taaaccgcac
     7741 ctgtccggtt aatgcggcat cgtccgcttc aaccatcgtc cacaacaccg catcggcata
     7801 atcgggattg tcccgattaa cctctatgct ttgcgcctcg ggaatcggca ggttttcctg
     7861 caaaagccct tcgagatgga acattgcggc ggctttggca tcggcaagcg cgtcggcaac
     7921 cgtatcgccg acaggatagc agcccggcag atcgggaata atcacgcccc agttgctttg
     7981 atcggggtct tggaacagca cggcgggata gtaaatcatt acttcctttc tcggatatgt
     8041 ggtatcggca aagtccaaac gcaacgaagg ggatttaaaa acgacaaaaa cgcaaaagcc
     8101 gccgacattc ccgcatccaa gtttcagtca atcagataac cttggatttc tttggttttc
     8161 gcattgattt ctctggtacg gcagtcagat tgtgccaccc cgtattcgtc gccgtcggcg
     8221 catttggcat ccaaaccgtt ttgccagttg cggtactcgg gcataacggt ttcggcaata
     8281 tcggcgggca gtgcctgaac cgcgctgttc agagccgctt tggcagttct ttcttgtcct
     8341 cttccctgac catttcgtcg ataagggctt ttttacggtt gtgcagctct tccaagcgtg
     8401 cttcggtttc tgccgtttta cacgccaact cactgacttt tatgccgttt ggcgtttcac
     8461 cttcggcttt cgcattgttg gcacagatct tgtccatacc gcttttccat gttttctgcg
     8521 aggcttgcag cttattctgt acagtttgag gcaatccttt ccaaaactgc tcgaactcgc
     8581 tgcgcgacag cttgtagcgt tcttcccatt cggatacgcg ggcggcttcc tgctcccgac
     8641 ccaatgcctc ctgcgccgcc gcttcttctg cctccctcgc ttcctgcgcg gtggcttctg
     8701 ccgcttcaag tttttcgttc ctttgtttcg ctttgtccaa cggctcttta atcagtgcca
     8761 tagacaccag ctcgctgagc ggatggatga tatcgtgtgc ctgactgagt tcggcaaaga
     8821 ttttgctttt gtcgtctgtc ggctggacgc tgtaagaaat agttttgaca taatacgcgc
     8881 cttctttgcg gtagtagggt tcaaaaaagt cgggcgtttt cttatggctg ttgcctatag
     8941 attggttggc ggcgacggca taatcgacaa catcgtcggg cacgtccagt tttaacgcgg
     9001 cttcacattt gagtttgctg gacgtgtcgg ttttctcggt agttcggact tcatcgacgg
     9061 ttatgcccaa acgttcgacc attctgcgga tatgcgatat gccgatatcg gcaaatgctt
     9121 ggtctttgaa tgcgtcgtcg tcaaaagttt tgacggtttc accttcgaca ttgtcgcgga
     9181 ccagtttgac caaaatctct ttagtctctt cgcttccgca ggaaatttcc tttccggaaa
     9241 acgggttgcc tattcccaaa cggtcgcagc ccgacaaaat cagcatactt gccaatgcca
     9301 gtgcgggtaa attttttttc attagatttc ctttgccgtc caaacacgga ctgtctgttg
     9361 cgtatttcca cacacgctac ggcacttgaa gtacatggct gcggaacata tatgtttaaa
     9421 tttatgcaaa ccgccatatc gggattgcac acgccctgca aatttaccga cggttttcct
     9481 gttcgataaa aatgccgtct gaaacggtcg gcgttcagac ggcatttttc cgcaggtttt
     9541 atttgcggtt ggtctgcagg tagaggttaa tcaggcgttc ggtcgagctg tcgtgttttt
     9601 gaggttcggt ttcgcctgtc agttcgccca aaatggtttt agccagttgt ttgccgagtt
     9661 ccacgcccca ctggtcgaag ctgttgatgc cccaaatgat gccttggacg aaggttttgt
     9721 gttcgtacat ggcaatcagg ctgcccatat tgcgggggtt gaccttatcc atcaggatga
     9781 ggttggtcgg gcggttgccg gagaaggttt tgtgcggaac cagctcttcg atgcgcgcct
     9841 catccatacc ctgcgctttg agttcggcgc ggacttcgtc gggggttttg ccgcacataa
     9901 aggcttctgc ttgtgcaaag acgttggcaa gcaggatttc gtggtgtccg ggcaggttgc
     9961 tgcgtttttc aagcgaggca atcaggtcga tgggggtgat gtgcgtgcct tgatgcagca
    10021 gttggaagaa ggcgtgctga ccgttaatgc ccgtttctcc ccagataatc ggcgaggttt
    10081 cgtgtccgac tgctttgccg tccaacgtaa cctgtttgcc gttgctttcc atatcgagct
    10141 gctggatgaa tttgggcagg cggtgcaaat gttggtcgta aggcgcgatg acgtggctgc
    10201 cgccaccgta gtagttgata taccagatgc cgatgagggc gaggatgacg ggcaggttgc
    10261 gctcgagcgg ggtgttgatg aagtgttggt ccatcaggtg cgcgccgttg agcatttcga
    10321 tgaagttttc ttcgccgaga tacagcataa tcggcaatcc gatggcggac cacaggctgt
    10381 accgaccgcc gacccaatcc caaaattcaa acatattggc gatgtcgatg ccgaattcgg
    10441 cgacggcttt tcgattggtg gaaacggcgg cgaagtgttt ggcaacggct tcttcgtcac
    10501 ccgcatgatt caaaaaccat tcgcgcgcgg ttagcgcgtt ggtcagcgtt tcctgcgtgg
    10561 taaatgtttt ggaggcgatg atgaacagcg tggtttccgg gtggactttg gacaatacgt
    10621 cgcgcagttg cgagccgtcc acgttggaga cgaagtgcat attgaggcgc ggatgaccga
    10681 aaggtttgag cgcggtacac atcgtcagcg ggcccaaatc cgatccgccg atgccgatgt
    10741 tgacaacgtc ggtaatgact tggttggtat agcccagcca gcttccgctg cggacttcgt
    10801 gtgcaaattc gcccatacgt tgcaaaacgc ggttgacttt gggcatcaca tcttcaccgt
    10861 caaccataat cggcgaattg gtgcggttgc gaagggcgac atgcaggacg gcgcggtttt
    10921 cggtggtatt gattttttcg ccgtggaaca tctgccgcat ccgctccggc acgcctgctt
    10981 cacgcgccaa ctcgaacagt agggacatgg tttcgtcgtt gatgcggttt ttggagtagt
    11041 ccagcgtcag tccgccgact tgcagccagt agcgttccgc ccgctgcggg tcttgctcga
    11101 acatttcgcg catatgcaat gttttgctgt cgtcaaagtg gttccacaat tttgaccagg
    11161 ccggtaagtc gtgaaggtgt ttcatctgta tgctcccgat tgaggttttc cgtgacgggg
    11221 ggcgtttccg cacatcgatg cgcacataag gcgcaaccct gcgtaccgcc ccgatttgga
    11281 aggggttgcg gcaaacccga accctgccgc cttaaagggc ggggtttccg ccattaagga
    11341 aattctgata aaaggcttgc cggagcctgt ccggccgttc agacggcatt ggcgggatgc
    11401 cgtctgaaca tcagtctttg tcgtaatcga tgtgcttgtt gtgtatgctt tttttgcttt
    11461 tctgcaattg caggctggca gcatcgccca agcgcagggc aagtccgatg gcgagaatat
    11521 caatgacggc aagctgcaag aggcgggaaa ccatgggcgt gtagagttcg gcgttttcct
    11581 gtgtggcaac gctcaacacg cagtcggcaa gttgcgccag aggcgaatcg ttgcgggtca
    11641 gtgcgatgac ggacgcgccg ttttctttgg cgatgctgac cgcatccaaa agttcgatag
    11701 acgaacccgt gttggaaatg gcaaccaaaa catcctgatc gctcaaaacg gatgccgcca
    11761 tcagttgcgt gtgcgtatcg acataggcga cggtggacat gccgaaacgg aaaaatttgt
    11821 gctgcgcgtc ctgtgccaca atgccggaat tgcccacgcc gtaaaattcg acgcggcggg
    11881 cgtgcatcag ggtggcgatg ccgttttcga gttcggattc ttttaagaac ctcctctccc
    11941 ccaacaaggc tgcggcggcg ttgcccaaca ctttttcaac cacattcgcc atatcgtcgt
    12001 cggcattgag ttcttcgtgg acataaggca taccttcgtg cccgatgctg gcggacaggg
    12061 agagcttgaa ttccggcagc cccttataac cgaggctgcg gcagaaccgg atgacggtcg
    12121 gctggctgac cgaggcgcgg tcggcgattt cggcgacggc ggcgtggaca aaccacttgg
    12181 gttctgccaa ggcgcattcg gcaactttgc gttccgcacc ggacaggtcg gccagtgatt
    12241 cgctgatttt gcttaacata atgatatgcc cttcgataat gcggccctgc tgcaagggct
    12301 gccgtggtta gacgtttttc aggtggttgc cgagcgccgc cgccgcgccg gcaatacccg
    12361 gaaattcgct caagacgaca taaacgggaa tcgcggcaag gtaggcttca aaacgccctt
    12421 tgttttcaaa gcggctgcgg aagggggagg ttttgaagta atccaacatt cgcgggataa
    12481 tgccgccgca caaatacacg cccccgcgcg cgcccaaagt cagggcgagg ttggaagcga
    12541 ccgtgcccag catcgcgcaa aaaatgtcca atgcctgccg gcacaggggc gattcgcaat
    12601 ttaatgcctt ttcggtgatt tcagacggca tcagttttgc cggttcggct ttctgcttgg
    12661 ccgccagcgt ttcataaatc aggctcaaac ctgcgccgct gaggaaacgt tcggcggaaa
    12721 cgtggcggta tttgtttttg gcgtactgcc aaatcaacac ttccatatcg tcaaacggcg
    12781 ggaaactggt atgcccgccc tcgcccgcca acgccaccca gcctgcgggg ctgtgcacca
    12841 atccgctcac gcccaagccg gtaccggggc cgataacggc cttgggggcg aattcgacag
    12901 gcttttgccc gcctacctgc atcaggtctt tgcttgaagt ctgcgttacc gccaatgcct
    12961 gcgcggtaaa gtcgttcaaa aggatgaggg tgtccagccc caaagcctga cgggtggttt
    13021 cgatggaaaa cgcccaatgg tggttggtca tctgcaccca gtcgcccaaa atcgggttgg
    13081 cgatggcaaa tgccgcgtgc cgtacgcccg ttgcaccact ttggttcaga taggcacgca
    13141 ccgcgtcggt aaccgtgtcg tattccttac acggcaggac ggcgactttc tcaatcacgc
    13201 acggcgcggt ttccaatgcg aaacgggcgt ttgtgccgcc gatgtcggca accagcctcg
    13261 ggtaatcggc gtgtttattc ggcgtagaag acatggcagt tcactccttg atggttcaaa
    13321 acgaggctga tcggatattc gcggttttcg ccttgtgcgg cttggtcgaa cacggctttt
    13381 ttctcttcgc cctgtatcgc caaaaataca tgcccagtat gggcaatcgc atccaaagtc
    13441 atgctgatgc gctcgtgcgg cgcggtaacg ggcgtggtat gcaccagcgc gacacctgcc
    13501 gaaccgtcga ttgccgtctg aaactgcgga gcttttggga aaatcgaagc cgtatgcccg
    13561 tcgtttccca tacccaaaat caaaacatcg ggctgcttgt aatgtttcag cgcataatcg
    13621 acaacagcat cgggatgtaa ttcggtttca gtttttccgt cttccaccat aggaatccac
    13681 actgccgcag cggctttgtt ctttaacagg tattcgcgca ccagaccggt attgctgtcg
    13741 gcgtggttgg tcggcacgat gcgttcatct gccaaggtaa ttccgacgtt tttccaatcc
    13801 aaatcttttt gcgacagggc gttgaaaaat gcaatcggcg aacgtccgcc ggaaactgcc
    13861 aacaccgcac cgcccttctc gtccagtgcg ccctgcaaag catccgccac tgcgtcagcc
    13921 aaagactgcg ccgcttctgc cgcattttcg tattcgtgcc aaacaaacat atttgtgtcc
    13981 ttttgtgatt tcagacggca tatcccgtta cagggcggct gccgggcaat ggcttagccg
    14041 cttccgcctc gaagcgggac tgctttttct gcccgccctc cttcagacgg cattgcgaac
    14101 ccgatgccgt ctgaagcgca ttactgttct tcgtgccact tgttgccgtc gcgcgccaac
    14161 agttcgcggg cggcttcagg gccccaagag tgtgcgccgt aaccgtgcgg cggcgtggtg
    14221 ttatttgccc agttttccaa aatcggcatc acatattccc acgcggcttc gagttcgtcg
    14281 cggcggttga acaaagcgag tttgccgtta atcacatcca acagcaggcg ttcgtaagcc
    14341 tcggcgcggc ggccttcaac ggccttgccc aaatccacgc ccaaaggcgt tacttccact
    14401 ttgttgcccg cacccggggt tttcacctgc gtgtaaaggc ggacggattc attcggttga
    14461 agctcgataa ccaaacggtt cggcgcggtt tggctgttgt cgaaaatatg gttttgcagc
    14521 gggcggaagt tcaaaacgat ttccgccact ttgcccgcca tgcgtttgcc ggtacgcagg
    14581 tagaagggaa cgcccttcca gcgttcgttt tcgatttcgg ctttaatggc gacgtaggtt
    14641 tcggtaaagc tgtcttgcgg aacgttgatt tcttcaagat agccgttcat gcctttggca
    14701 gcggtatatt gtccgcgcac gacattttca ttgacagatt cgatggtcag cggcttcaat
    14761 gacttgatga ctttgacttt ttcatcgcgc accgcgtcgg catccaagct ggcgggggct
    14821 tccatcgcag tcatgcacag catctgcatc aaatggtttt gcaccatatc gcgcaacgcg
    14881 ccggtaatgt cgtaaaactc gccgcgctct tccacaccga gctgttcggc gatggtcagc
    14941 tgcacgcttt cgatgtattt attgttccac agcggctcga acattacatt ggcaaaacgc
    15001 agcgcaagca ggttttgcag gctttctttg ccaaggtagt ggtcgatgcg gtaaatttgc
    15061 ccttctttga aataacgcgc cacatcggta ttgatttgtt gggaagaagc caaatccgta
    15121 cccagcggtt tttccaaaac cacgcgcaca ttgtcggcat tcaaaccgat cgcagcaagg
    15181 ttttcgcagg cttgcgcgaa gaatttcggc gcggtggaca gatagatgac gacgttgtcg
    15241 gtttctttgc gtgctttgac caaatcgccc aaagcggcaa aatcgtccgg ctgcgtaaca
    15301 tcgactttga gatatgcgag acgttcaaca aacgaagccc acgcctcacc ggaaaaattt
    15361 tctttcacat ggattttgga actggtttcc accttcgcca gaaaaccttc ggtatccaac
    15421 tcgctgcggc tgacccccaa aatacgccct tcgggatgaa gcagaccggc aacatgcgcc
    15481 tgatacaggc agggcaaaag tttgcgcatt gccaaatcgc cggtcgcacc gaacaacacc
    15541 aaatcaaaat ttgtctgtgt actcatcgca ttatctcgtc aggaaaggat tttttcgatg
    15601 ccgtctgaaa cctgtttttt ccatcacgca gcatcgcaat atcggaaaca aaggcaggcg
    15661 gcataatgag tagtaatact acacaccgct acactttttg tctattccca tttttacaat
    15721 ttatttgacc tagtccaaaa atcaggcagg tttcccctat tccgttacaa caatcagaaa
    15781 attctgcgat ttaaatcaaa tttcttttca atgtctgatt ttttgtaaca aaattacaaa
    15841 ttttgtacta taatagcacc cgcttcccac tttcagacgg cgtacctttt
//
LOCUS       DAXFWM010000058        15564 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000058 DAXFWM010000000
VERSION     DAXFWM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.058"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            349..768
                     /locus_tag="ACFI8B_001659"
     CDS             349..768
                     /locus_tag="ACFI8B_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834829.1"
                     /translation="MNETDLPKLLSVINRFTDIDKNWSCVLLFWIQRASGYLEMMTLD
                     DDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEILFLMAQYGSEYWNSLEESF
                     EEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            858..3317
                     /gene="mobH"
                     /locus_tag="ACFI8B_001660"
     CDS             858..3317
                     /gene="mobH"
                     /locus_tag="ACFI8B_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFC3834830.1"
                     /translation="MSHNDEVAHINQNIVNDRFRILNAHELIQVLDLSPQISGIKMNL
                     GLSDENWSKDALPFLEKYIAFVQRLPASESHHHAGDGGLVRHTLDVAALALVASTSQS
                     WPPNAKTEEIAKKTAVWRYGIMCAALLHDVGKTVTGFQVELFDSAISLEKLLWLPDTG
                     SMAESGKLYYRVEFPDAKSAYSTHAEIAWTFFQALVPSHVRQWLATTDPNLMITLRNY
                     LSGKKDGSPLEQLIKNADMTSVSRDLRSGSRQRFSTAKRKPFIETIMETLKEMLSDRG
                     VHFSIATTAGGDLFRKGDRIYLMSKNVPDYIRQYLRKNQHPAAGSFPADNQRIFDTLF
                     EYRAVIPPENDPHRAINHIEVEFTRMDGEKVRNIFSVLCFNAKTLYPDGDYPTEFLGN
                     LSEVSAKKEIPDVVVSEDKVSTQIQGAEDNPVTMTKDKMDFLSIQPVTAEQPPLPEKN
                     QTETANSAFDNSMNTAAPAENKSSESEKAKEPGYGTIDNLIENFNLIEADSEQTESTK
                     SEAETAENAVNLVQENIRVEVKTETVDKPRAEVKETETDKAPKTIKSGTKASRKKLAG
                     LFADNRKPIGKTAEPDAQIKTTETVTHQIDSEAVSLPESESAQNGMRELERLRLREPT
                     SSPRPVQVMDDSEGLDSLASGVKNMAELEAIVSERNLKKQSETESTEIVKDSVKAKHM
                     EARDRGMQFLRWLADGLGDGSIAVNRSGATVHFIEQGMLLVTPAVFRDYAGGVFNKSD
                     TESLGPLTQKGFEALRLHARTKKSSLHRVLTTNGSNRRLFYCYLIPEHNIKHIIQPGS
                     RPQNNTDIKLDESDLLVMEQK"
     gene            3314..5356
                     /gene="traD"
                     /locus_tag="ACFI8B_001661"
     CDS             3314..5356
                     /gene="traD"
                     /locus_tag="ACFI8B_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFC3834831.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRTLSALRIMRFRKNIRKLPYYALTADEIPSSDKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            5346..6041
                     /locus_tag="ACFI8B_001662"
     CDS             5346..6041
                     /locus_tag="ACFI8B_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFC3834832.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWSESVGHDAFPGKEVGDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPPYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(6486..6998)
                     /locus_tag="ACFI8B_001663"
     CDS             complement(6486..6998)
                     /locus_tag="ACFI8B_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834833.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYILPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(7089..7484)
                     /locus_tag="ACFI8B_001664"
     CDS             complement(7089..7484)
                     /locus_tag="ACFI8B_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3834834.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGLL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(7471..9393)
                     /locus_tag="ACFI8B_001665"
     CDS             complement(7471..9393)
                     /locus_tag="ACFI8B_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3834835.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(9470..9841)
                     /locus_tag="ACFI8B_001666"
     CDS             complement(9470..9841)
                     /locus_tag="ACFI8B_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC3834836.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            10037..11344
                     /locus_tag="ACFI8B_001667"
     CDS             10037..11344
                     /locus_tag="ACFI8B_001667"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC3834837.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            11337..11771
                     /locus_tag="ACFI8B_001668"
     CDS             11337..11771
                     /locus_tag="ACFI8B_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834838.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(11947..12216)
                     /locus_tag="ACFI8B_001669"
     CDS             complement(11947..12216)
                     /locus_tag="ACFI8B_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC3834839.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(12398..14860)
                     /gene="lon"
                     /locus_tag="ACFI8B_001670"
     CDS             complement(12398..14860)
                     /gene="lon"
                     /locus_tag="ACFI8B_001670"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC3834840.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            15112..>15564
                     /locus_tag="ACFI8B_001671"
     CDS             15112..>15564
                     /locus_tag="ACFI8B_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC3834841.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
BASE COUNT         4028 a         3617 c         3781 g         4138 t
ORIGIN      
        1 atattgatat aaaaaggaat acgataaggg taaaggctct tatccaaaag ccaaaaggcc
       61 gtctgctaaa aaaaatcgca gccggccttt tttaaaagct gtaacggtaa agcaacagcc
      121 ttaatggtta aaggaagggt tcaagggaaa aagggtaaaa gttaaaggta aaacaaaggc
      181 ctgcatgtaa aaatgcaggc ctttgcctat taaaaggggc agcattatac cttataaatc
      241 ttattctgtg acaattctaa ttaaaataac attttttatt ttgatttttg acatggcaac
      301 ttttttttat tatatgaatg gtttgacaac cctgtctttt taatcaaaat gaatgaaaca
      361 gatcttccca agcttcttag tgtcataaat cggtttacgg atatcgataa aaactggtct
      421 tgcgtgttgt tgttctggat acagcgtgct agcggttatc tggaaatgat gacgctggat
      481 gacgatgaaa atacggcaac cttccttatc gaaaaactag agaaactact agagccactg
      541 ccgatagata ttgataacac gacttttgca gaagcacttg ccgatgactt tgaaatctta
      601 tttctaatgg cccaatacgg ttctgaatac tggaattcac ttgaagaaag ctttgaggaa
      661 ttttgtatta gacatactac gctacctaat caattgattg ctgatattcc acatgcaaaa
      721 aaggaaaaag tcaccatgtg gctaaaatct ttgcttaaat catcatgaaa acaagccttc
      781 tcactattgc atcctcacta atgcttgtca gcggctcaat gctatacctg ataaatacga
      841 gaaaaagtac agattccgtg tcacataatg atgaggttgc ccatatcaat cagaacatag
      901 taaatgaccg gtttcgcatt ttaaacgcgc atgaactgat tcaggtgctc gacttaagcc
      961 ctcaaatatc cggtattaag atgaacttag ggttatcgga tgaaaactgg agtaaggatg
     1021 cccttccctt tttggaaaaa tatatagcct ttgttcaaag gcttcccgcc agtgagtctc
     1081 atcaccatgc aggtgatggt ggtttagtcc gccatacact cgatgtggct gcactggctc
     1141 ttgtggcatc tacatcgcaa tcatggcccc caaatgccaa aaccgaggaa atagcgaaga
     1201 aaactgccgt ttggcgttat ggaatcatgt gtgccgccct gcttcacgac gttggtaaaa
     1261 ctgtgacggg atttcaggtt gaactgtttg attcggctat cagtctcgaa aaattattat
     1321 ggttgcctga tacaggctcc atggctgaaa gtggcaagct ttattatcgt gtcgaatttc
     1381 ctgatgcaaa gtctgcttac agtacacatg ccgaaatcgc ctggacattc tttcaagctt
     1441 tggttccttc acatgtcaga caatggcttg ccacaacgga tccgaattta atgattactt
     1501 tgagaaatta tctgagtggg aaaaaggatg gcagtcctct tgagcagtta ataaaaaatg
     1561 cggatatgac ctcagtttca agggatttac gaagcggcag ccgtcaacgc ttctcaaccg
     1621 ccaagcgtaa accattcatt gaaaccatta tggaaacatt gaaggagatg ttatccgata
     1681 gaggagtcca tttcagcatc gcaacaactg ccggtggtga cctgttccgt aagggtgatc
     1741 gcatctattt gatgtcaaaa aacgtacctg actacatccg gcagtatttg aggaaaaatc
     1801 aacatcctgc tgcaggctca tttccggctg acaaccaaag gatatttgac acattattcg
     1861 agtaccgtgc cgtcattccg ccggaaaacg atcctcacag ggcaattaac catatagagg
     1921 ttgagtttac ccgtatggac ggtgaaaagg tccgtaatat cttcagtgta ctttgtttca
     1981 atgccaaaac cctgtatccg gacggagatt atccgactga gttcttaggt aatctttcag
     2041 aagtttccgc caagaaggaa ataccggatg ttgtagtatc agaggataaa gtgtcaactc
     2101 agatacaagg tgccgaagat aacccagtca ctatgaccaa ggacaaaatg gacttcctgt
     2161 ctatccaacc ggtgacagca gaacaacctc ccctacctga aaaaaatcaa actgagacag
     2221 ctaactcggc ttttgacaac agtatgaata ctgctgctcc agcggaaaat aaatctagcg
     2281 agtcagagaa agctaaagaa ccgggttacg gcacaattga taatttaatt gaaaacttta
     2341 acctgataga agctgattca gaacaaacag aatcaaccaa atcagaagcc gagacagcag
     2401 aaaatgccgt taacctggtt caagaaaata taagagtaga agttaaaact gagacagtgg
     2461 acaaacctcg cgctgaagtt aaagaaactg agacagataa agcaccaaaa acaattaaat
     2521 caggaaccaa agcaagccgt aagaaacttg ccggcctttt cgcagacaat cgtaaaccta
     2581 ttggaaaaac agccgaaccc gatgcacaga ttaaaacaac tgagacagta actcatcaaa
     2641 tagattctga agcagtttca ttgcctgaat ctgaatcagc acaaaacggt atgcgtgaac
     2701 tggagcggct aagactaaga gaacctacat ctagtccacg cccggttcaa gtcatggatg
     2761 actccgaagg tttagatagc cttgcaagcg gggtaaaaaa tatggccgaa ctcgaagcca
     2821 tagtttctga acgtaatttg aaaaagcaat cagaaacaga gtcgaccgaa atagtcaagg
     2881 attcagtcaa agccaaacat atggaggcca gagaccgtgg aatgcagttt ttacgatggc
     2941 tggcagatgg attaggagac ggcagcatag cagtcaatag aagtggtgca acagtccact
     3001 ttattgaaca ggggatgctt ttggttacgc cagccgtatt tcgagattac gccggcggcg
     3061 tgttcaataa atctgatacg gaaagtctag gccctcttac acaaaaaggc tttgaggctt
     3121 tgaggttgca tgcaagaacg aaaaaaagct cattgcatag ggttctgact acaaacggta
     3181 gcaatcgcag actcttctat tgctacctta tccctgaaca taatatcaaa catatcatac
     3241 agccaggttc aagacctcag aataataccg atattaaatt agatgaatcc gacttattag
     3301 taatggaaca aaaatgagtg cccacttccc tgaaaacgtt tatcgatcac cctatgaatg
     3361 gatcagtgta gccgcatact ccaccacagc ggtttcagta gcacatttgc ccgtacctcc
     3421 gacaattgct tatggcagtt tagctatttt ttcgttggtt tcagtcaaac gcacgttatc
     3481 agcattacgc attatgcgtt tccgtaaaaa tattcggaag ttgccctatt atgcccttac
     3541 tgccgatgaa attccttctt ctgataaagc tctttttctt gggaaaggtt ttagatggac
     3601 acagacacat acccaacgtc tttttatggt tcgccagcct gagaacgacg gacttcgtaa
     3661 cccctcaaaa ctctataagg cagcgcgaaa acatgagatt gagacaatgg gtacagactg
     3721 gatttcccgc ataactcgga aatcggcatg gtggaatccg gttgctccct tacctccggt
     3781 cggaggttct cctgaattac atggcattga aattgaggaa gatgatattt gggaagattt
     3841 gggggagcgt gttggccaca aggttgtatt gggcacaacc cgcgttggta aaacccgatt
     3901 atgcgaaacc atgattgttc aggatattcg gaggggggat gttacgatcg catttgatcc
     3961 aaagggtgac gctgacctga tgactagaat gtatgtcgaa tcgcaaaaat caaacaggcc
     4021 gttttatatg ttccatttgg gtttccccga gtacagttgc cggtacaacc ctatcgggga
     4081 ttatggccgt attaccgagg tggcgacaag gattgcaaac caactgcctt ctgaaggtca
     4141 gtctgctgct ttccgagatt ttgtatggcg tttcgtcaac gttttaacga aaaccatgga
     4201 aggtttatcc attaaaccaa gctatgaaaa tatctataag tctgctgcaa atgtagatga
     4261 acttgcaggg gattacttta aaaagatatt agataaacac caccctggtt gggagaaaga
     4321 cttcgacaat tttgatatgc ccgagtctga agcaaaaagc gcagtcaaaa ctgggcgcaa
     4381 cttggacaca atgaagctcg gttctttctt gaaggccaaa aagcataacg aacctcttat
     4441 tgacgcattg ggtggtattt tgtccaatga cagatcctat tttgagaagc tagtcagctc
     4501 cctgtatcca ttgctggaaa aactgacgac tggcgaagtt gccaagctta tcagcccgga
     4561 tacagaggat ttgtcagatc caagaatcat tatggactgg cgcaaagtca tggaatcgaa
     4621 tgccgtcgtc tatattggat tggattccct atctgatgcc gaagttgcag cagccgttgg
     4681 taatgcgatg tttgctgact taacctctct tgcgggtcag atctataaat tcggccatgg
     4741 ggttggacag agtggaaaaa cccagaaaag aaaagtctca attcatgcag acgaattcaa
     4801 tgaattaatc ggtgacgaat tcattccgtt attgaataag gccggtggtg ccggctacca
     4861 agtgacggtt tatacacaaa cctggaaaga cgtagaagcc aaaattggtt cccctgctaa
     4921 agcaggtcaa attggcggca acttgaatac gatgattatg cttagggtga aaactgtcga
     4981 aacagcagag atgctgacga atcagttgcc ggaagtcaaa attatgacca gcactctagt
     5041 ctcaagggct ggtgatgttg ccttccctga tagtcacgaa gattttagca gtggaaacga
     5101 agacaggatt ggtgctgaga cagttcctat gctgacaccc gccgatttaa cccaattacc
     5161 caaaggtcaa gcattcgcgc ttattgaagg tggccagcta tataaaattc gactccctct
     5221 acccaaaaag gacaagcagg aggaaatccc tgcgcatatt ggagaaatgg cggctgatat
     5281 gcgcgaagtt tacagactaa gccggccttc ggctgaagaa aacgcattta cggagggaag
     5341 tagttatgcc gtctaataca agcgcatggt acatgaattc attgccttta aagttcttat
     5401 ttctcccgtt taaagctgct tataccttat tcttaattgt gctgcttatc tttggtagtt
     5461 cggtgttgac acaaaactat tttggtaccg gtaatctatt ggaagatgaa atcaaatggt
     5521 ctgagtctgt tggccatgat gcgtttccag gaaaagaggt tggcgatact ataaatctgc
     5581 aaattgccag acttagttac aaggggctga aaaccgtatt ttttgacctg acattagtca
     5641 atgaatcact gaaagccaag tcagaagccg attttggtta caaatttaaa aattatttag
     5701 tcccccgtat gaaatatcta cagcaattcg atactacgtt gaaggttatt tccttacgtt
     5761 tgggaaattt ggcattaatc gtgggtatct cagtaatctt tgcaattccg gttttgatag
     5821 atggtttagt aatgagagcc attagacagg aaaatgcctc gcgagagagt gcaggcattt
     5881 atcaccgtgc aaaatactgg agaacaggga ttatttggtt gggttgcatg atttatatgt
     5941 gtgtgccaat atcaataccg ccctacttgc tctatttacc tctggtagca gcaatttgga
     6001 tgattttcat gcaagctaaa tatcttaaga aatatctcta ggcttccagt agaatctaaa
     6061 tagtgctttt ttagttcatt aaacgggggc ttaatgcttt aactgacggt acagaaatct
     6121 cgaaaagcat tttgcaaaat acttaaaata agagtatagt aaaaatgaaa tatttcaagt
     6181 ttaactaata gttaacaatc tagagtaaac aatcgtaatc gttaaatctg ggtatagatt
     6241 gcataatata cattatgcga actatcggaa acagttgtac gtgtagccgg tattggggaa
     6301 tacgttgacc ttcaatacga ttacaggcag ccgacacaca ggatatgccg cccatacttt
     6361 tttaatccaa aacattatat caacgggcgc aaacagtaaa tcgcccaact tatcccaaaa
     6421 attctcctgc tttttcatca tttcttctgt caaatccttg gaacggtcga tttgaaaaca
     6481 cggccttaca tacgtcccaa ttctctttgc agtgcttgga tattttgctg tctgtccaaa
     6541 acattgcttt gcaatgcgtt gattttttga tggttgatgt tgccgccttt tgccagacgt
     6601 gcttgtgata acattttttg ggcttcagtc agggctttgc gttcattgct taattctgct
     6661 tcgagaatgg agcgtctgct gttatttaca ggtgcttgtt gaggcggcgt attggatttt
     6721 gccggcttgg atactgtttt gaccggggct ttatatttga cagcctgtcc gccgtttgac
     6781 ggtgatggtg ccggttcggg agtttggggc aggatatagc gttcgctgct gtagttgccg
     6841 attgggggca aatcggttga gtggcagctt ttagacggct tggtggtgta aacggtttct
     6901 ccgttgattg tgcaggtata gattttggcc gcattcgcac ccaatgggct tgaaatcagg
     6961 gaaaggttga ttaggattaa ggggagtttt gatttcataa tgtgtttgat attcggataa
     7021 taatttgatt ttttgaggat tttttgtaag gttttcttgg cgttttatgg gcttttcttt
     7081 tcggttgttt atttttgatt ttccttcggg gctttggcag cctgtctgtt ctttttcccg
     7141 gcatgttttt tgtttttctt tttgtttcct gtgtgtattt tggcttcttg actaagtttt
     7201 ttaattttca ggcggtatcc gcctccctga tggctgctga ttttaggtaa atcagcatcg
     7261 gcacacgatc ctgacgggtc tgatgtatat acgcttttgc catttgagtt gcaatggtat
     7321 atggaggctt gcgccgccgc gccggaaagg gacaggagac ccaaggcggc aaaaagtctg
     7381 atgtttatac cggtaaagta aaaaggcggt ggtaacgtcc gaattatacc gttattggca
     7441 ggcaaagata agcaccctgc ccgcgcttct ttatcgctcg gcaaactgtt tctcgatttc
     7501 agtttcaatc cgttccaatt cttcttgaca agccagccaa gtttcttcga tttgggcaag
     7561 ctgtgttttg acttttgcca gctcggataa ggtattttgc aatttttctt tgtttgcttc
     7621 aaggtaagcg tcttcttgtg ttaaaaatgc ttcgcatccc gtctgaattt cagaaagctg
     7681 caccatttct ttttcggctt tgtcgatttt ctgttgtatc ggcttactgc gtcgggcttt
     7741 ttcttgacgg atttgcgctt cgatacgctt ggtatccttg cggttttggc tttgagtgga
     7801 agcggctggc gcggttgcgg cgttttcctg tgccaagcgc cattggcggt aatcgttcaa
     7861 atcgccgtca aagtttttca gacgaccttt gtctatcaag aggaaactgt cggtcgtggc
     7921 ttcaagcagg ctgcggtcgt gcgatacgac gattaatgca ccttggaaac tttgcaacgc
     7981 gagcgttaag gcgtggcgca tatccaaatc cagatggttg gtcggttcgt caagcagcag
     8041 caggttcggc ttttgccaga taatcatggc aagggcaagg cgggcttttt ctccgccgga
     8101 aaaaggttcg gttttctgca acgccatatc gccgacaaag ttgaagccgc cgaggaaatt
     8161 tcggatttct tgttcgcgta cttcgggaga aagctgctgt atatgccaaa cagggctttg
     8221 gtcggcgcgg atggtgtcaa gctggtgttg ggcgaaatag ccgatattaa gtttttcgga
     8281 atggacgatg ctgccggaga gtaaatcgat ttttcctgcc aaagctttga taaacgtaga
     8341 tttaccgctg ccgttgacac ccaatagccc gtaacgtgcg ccgctttcca gcgacagggt
     8401 aatgtcatgc aggacggttt taccttcgta acccaaatca gcgtgctcca gcttcagcaa
     8461 aggattgggc agatggtcgg gattgtaaaa ctcaaaggaa aactcgctgt ccagatgtgc
     8521 gggagcgatg cgttcgagct tcgccaacgc cttcatgcgg ctttgagctt gaacggcttt
     8581 agtggctttg gctttgaagc ggtcgataaa ggattgcaga tgtttgattt gcgcctgctg
     8641 tttgacatat gcggcttgtt gttgcgccag acgctgcgcg cgttcggttt ggtaaaaatc
     8701 gtaattgccg ctgtattgcg tgagcttttg ctgcgacaat tcaatggttt gggtggtggc
     8761 cgcattgagg aaatcacggt cgtgggaaat gatgatttgc gtgcagggta aagaggcaag
     8821 gtggttttcc aaccacaaga cagtttccaa atccaaatgg ttggtcggtt cgtcaagcaa
     8881 gagcaaatca gcgcggcaaa tcagggcttg cgcaagattc aggcgcatac gccagccgcc
     8941 ggaaaaggat ttgacgggac ggctgtgttc ttcttgcgaa aaacccagcc cgttcaacaa
     9001 ttttgccgcg cgggccggcg cggtataggc gtcgatttct tccaatttgg catgatattc
     9061 cgcctgcttc atgccgtcat tttgcacttc cgcctgcctc aatgccgtct gaaaagcctg
     9121 caactcggca tcgccctgca aaacgtaatc caaagcggaa atatccaaat cgggcgtttc
     9181 ttgggaaacg gaagcgagcc gccagttttt cggaatcgag atatcgccgc cgtcctgagt
     9241 gatttcgccc ttgattaagg caaacaggct ggatttgccc gtcccgttct taccgatcaa
     9301 accgacgcgc tgaccgggat tgacggcagc ggaagccttg tcgagcagga ctttcaaacc
     9361 gcgttgcagg gtgaggtttt tgatttcaat cataacggaa acatcgtcgg gcgggaaaag
     9421 cccgtatttt acctgaaagt cagtgccgat gccgtctgaa acgggaaatt tacggctgaa
     9481 gccaagccca agccctgcgc ccttccgagt gcaggaaaac caatgtcctg aatgccgaat
     9541 cggtattcat gcattccacg ctgattccga ttcgggaaaa atccgccatg attttgggat
     9601 ggataaactc ctgagccgcg cccgtcccga taatcaatat ttccggatag tcaacaggtt
     9661 tgatgtcgga caacaggttt tccggagtca ggtcggacaa ggttcggcat tgcggcaggc
     9721 agaccgaatc cttatgtaca agcacgggtt tatggaaact ttgccccgcc agccggattc
     9781 cgcccgcgcc gcattcatat tctgcaaact gtccgtctat cggattttct tcaaacaaca
     9841 ttttttaccc cgttgctgca tcatctgcac cgaaaggggt gcaaaatcag gcgaattaat
     9901 gtaggattgg cagatttcat ctgacgcgcc tgccgatttc agacggcatt tgattcaaag
     9961 tgcggcacaa ttataccggc agcggatatt ttcgtctttc aatatttaca tttcagtcgg
    10021 cttacaagga gacacaatga agccagtaaa catcggtctt ttaggcttag gtacggtcgg
    10081 cggcggtgcg gctgccgtgt tgcgggacaa cgcagaggaa atttcccgcc gcttggggcg
    10141 cgaaatccgt atttctgcca tgtgcgactt aagcgaagaa aaagcccgcc aaatctgccc
    10201 gtccgccgcc tttgtcaaag atccgttcga actggtcgcg cgtaaagacg tcgatgtcgt
    10261 cgtcgaattg ttcggcggca cgggcattgc caaagaagcg gtgttgaaag ccattgaaaa
    10321 cggcaaacac atcgttaccg ccaacaaaaa actgctcgcc gaatacggca acgaaatctt
    10381 cccgctggcg gaaaaacaaa acgtcatcgt ccaatttgaa gcggcagtag cgggcggtat
    10441 cccaatcatc aaagccctgc gcgaaggttt ggcggcaaac aggattaaat ccatcgccgg
    10501 cattattaac ggcaccagca acttcatcct ctccgaaatg cgcgaaaaag gcagcgcgtt
    10561 tgccgacgta ttgaaagaag cgcaggcatt gggttatgcc gaagccgacc cgaccttcga
    10621 catcgaaggc aacgacgcag gccataaaat caccatcatg agcgcgctgg cattcggtac
    10681 gccgatgaac ttctccgcct gctatctcga aggcatcagc aaactcgaca gccgcgacat
    10741 caaatacgcc gaagaactcg gctaccgcat caaactgttg ggcatcaccc gcaaaaccgg
    10801 caaaggcatc gaattgcgcg tccatccgac cctgattccc gaaagccgcc tcttggcaaa
    10861 cgtcgacggc gtgatgaacg ccgtgcgcgt caacgccaat atggtcggcg aaaccttata
    10921 ttacggcgcg ggcgcgggcg cattgccgac cgcttccgcc gtggttgccg atatcatcga
    10981 catcgcccgc ctggttgaag ccgacaccgc ccaccgcgta ccgcatctgg cgttccaacc
    11041 cgcccaagtc caagcgcaaa ccatcctgcc tatggacgaa attaccagca gctactacct
    11101 gcgcgtccaa gccaaagacg aaccgggcac gctggggcaa atcgccgcgc tgttggcaca
    11161 agaaaacgtg tccatcgaag ccttaatcca aaaaggcgtt atcgatcaga ccactgccga
    11221 aatcgtgatt ctgacccaca gcacggtcga aaaacacatc aagtcggcaa tcgcagccat
    11281 cgaagcactg gattgtgtgg aaaaaccgat taccatgatc cgcatggaaa gcctgcatga
    11341 ctgagccgaa acacgaaacg ccgacggaag agcaggttgc cgcgcgcaaa aaagcaaaag
    11401 ccaaaatccg caccatccgc atttgggcgt gggtcatttt ggcgttgctc gcttcaaccg
    11461 ccctgctctc ccaatgcgcg atgtccaaac cgcaggcaaa acagaaaatt gtcgagtctt
    11521 gcatgaaaaa tattccgttt gctgaaaaat ggcagaacga tttgaaagcg cgcggcttgg
    11581 atgcggacaa tacccgtctc gccgtcgact actgcaaatg tatgtgggag cagcctttgg
    11641 acggattgag cgagaaacag atcagctcct tcggcaaact cggtgcacaa gaacagcttg
    11701 acctgctcgg cggcgcaaac gcgtttgaaa ctcgagacaa acaatgtgtc gcggatttga
    11761 aagccgattg aacctcaggt ctggaatgcc gtctgaacgc ggggaaaagc gtttcagatg
    11821 gcatttttcc atccgggttt gacgggcagc ccttcatccg aatccgacgg gcaaacccct
    11881 atcctgattc ggcggggaaa acaaaaagcc gattcttcaa ggaatcggct tttgctgaaa
    11941 aacagtttac agtgcgtctt tcaatgcttt acctgcacgg aatttaagcg ttttggcggc
    12001 ggcaatggtc agaggctcgc cggttttggg gttgcggcct tggcgttccg cacgttcgcc
    12061 gacgtagaaa gtaccgaaac cgaccagagt aacggtgtcg ccttgtttca gggcgttggt
    12121 tactgcgttg gtagtggcat ccaaagcttt ttgtgcggcg gctttggaaa tatcggcttc
    12181 ttgagcaatc gcttcgatca attcagactt gttcacaatc agtcccttcc tgtcttaaaa
    12241 aatgatgaaa tgcccgaata ctcggggttt gtactgcttg agcaactttc gctttatagc
    12301 aattctgaaa ttgccgtgtc aagcaaaaaa tacggaatcg ccctatttta caggctttca
    12361 ggacgaaacc gcatttttac aacacatttc ctgcgtttca atgtttgatt gccctgctgc
    12421 ggggtttcgg ttttgaagcg gattccgccg ccgcttccgc ggcagaaggt tctgcccaag
    12481 actcaggccg gctttccaaa cccaaagcca atacctcgtc tatccatttg accggatgga
    12541 tggttaatcc ggttttcacg ttttcaggga tttcttccaa gtctttgaca ttgtctttcg
    12601 gaatcaggac gtgtttgatg ccgccgcgaa gcgcagccaa cagtttttcc ttcagaccgc
    12661 cgatcggcag gacttcgccg cgcagggtaa tttcgcccgt catggcaaca tcggcacgca
    12721 ccggaatttt ggtaaaggcg gacaccatcg ccaaagtcat agcaatgcct gcgctcggac
    12781 cgtctttcgg cgttgcacct tcgggaacat ggacatgaat gtcttttttc tcgtaaaaat
    12841 caggagccaa acctactgat tccgcacggg aacggacaac agaccatgca gcggatacag
    12901 attccttcat cacatcgccc aactggccgg tacactggat catgccttta cccggtaatg
    12961 ctgcggcttc gacagtcagc aattcgccgc cgacttccgt ccaagccaaa ccggtaacct
    13021 gtccgatgcg gttttcactt tcggcaacgc cgtaatcgaa gcggcgcacg cccaagtagt
    13081 catgtaggtt tttctcattg actttaaccg ccctaggctt ggctttgctg gttttcttgg
    13141 tttcagacaa cctcttctta tcttcgttca aggtaatctg catcaccacc ttacggcaga
    13201 ttttggcaat ttcgcggtcg agcgaacgca cgcccgcctc tcgggtgtaa taacggataa
    13261 tatcgcgtac cgcgctttct tcgaccacca attcgccttc tttcacgccg ttgcgtttca
    13321 tttgcttcgg tacgagatac tgcatcgcga tattgatttt ctcgtcttcg gtatagccgg
    13381 acagacggat gatttccata cggtcaagca gcggggtcgg gatattcagg ctgtttgaag
    13441 tcgcaataaa catcacgtcg ctcaaatcgt aatccacttc ggcataatga tcggcgaact
    13501 tgttgttttg ttcgtgatcg agcacttcaa gcaacgcgct ggcgggatcg ccccgaaagt
    13561 cgctgcccaa tttgtcgatt tcgtcgagca ggaacaacgg gtttcttacg cctgcttttg
    13621 ccatattctg caaaatcttg ccgggcatag agccgatata ggtacggcgg tgcccgcgga
    13681 tttcgctttc gtcgcgcacg ccgcccaagg ccatacggac atatttccgc cccgttgctt
    13741 tggcgatgga ttcgcccaaa gaggttttgc ccacgcccgg agggccgacc aaacacagaa
    13801 tcggaccttt gagcttgtcc atacgttttt ggacggcgag gtattccaaa atccgttctt
    13861 tgactttttc caggccgtag tggtcggcat ccagcaccag tccggctttg gcgatgtctt
    13921 tgctgacgcg ggattttttc ttccacggca gtccgagcag ggtgtcgatg tagttgcgta
    13981 cgacggtgga ttcggcagac atcggcggca tcattttgag ttttttcagt tcggacaggc
    14041 atttttcttc cgcttctttg gtcatacccg cctttttgat gcctgcctcc aaggcatcca
    14101 gttcgccgtt ttcgtcttct tcgcccaatt ctttgtgtat cgctttaatc tgttcgttca
    14161 gataatattc gcgttgggat ttttccattt ggcgtttgac gcgtccgcgt atgcgttttt
    14221 cggcctgcat aatgtcgagt tcggattcca actttgccag caggaattcc atccgtttgc
    14281 cgatttcggg aatctccaaa atctgttggc gttgcgccag tttcaactgc aaatgcgctg
    14341 cgaccgtatc ggttagccgg ctgttttcgg caatgccgtt gatgctgccg ataatttcgg
    14401 cagggatttt tttattgagt ttggcgtatt gttcaaactg cgccaacagg gtgcggcgca
    14461 cggcttcgag gtcggtattg ccgcccgtgt cttcttccac gaccgcctct atatgagaaa
    14521 cgaacagccc gcccgtgtct tcgatggtca aaacacgtcc gcgatacagc ccttcgacca
    14581 gcacttttac cgtgccgtcg ggcagtttca acacttgcag gacttgtgcg acggtaccgg
    14641 tctgatacag gtcggcggca accggttctt ctaccgccgc atcggtttgc gccaacagga
    14701 aaaccggttc ctcgcgggta atggcgtttt ccagtgcggc gatggatttc ggcctgccga
    14761 cgaacagcgg cagaaccata tgcgggtaaa cgacgacatc ccgtaaagga agggtcgcca
    14821 aagcagcata ttcctcaaaa tgcttttctt tttgtgtcat ggatactctc ttgtgtctga
    14881 caggatacgg attgccgcgt aaattggggt tgaaagtatt atttcaagca tacgcggttt
    14941 atttatggag tttgatgcca tgccgtctga aacattccgg cttcagacgg catgggcttg
    15001 gaaagacaag gcgggaacaa aaaaacagtt ctgtgttgcc gctccttgct gtaccatccg
    15061 tatggtttgc ggttctgccg ccctattttc aaaacatgac gcaggtatcc catgtcttct
    15121 tttatttttc ccgataacgg gttcgaatgg cgcaacgaaa gtctgcaaaa gccgcccaaa
    15181 ggttggtatt atgtccgcga aagcggtgca gacggcattt tgaaggctac ctatcaaaat
    15241 attgcaactg tctggcaggg cgatttccac aatgccaaac aggtgctttc ggcaatgaag
    15301 aagaaggtcc gcaaacctgc cgccgtccgt tccgatgcgg atatcgccgc ccttttccac
    15361 gcccaccgta tgaagcaggc gcaacagagc cgtattctga atatgcttgc cgttgaaatc
    15421 cgccccggtt tcgtgttgga caacaaacgc gcgcccgata tacgcgccgc tttgctcgac
    15481 gtgtacggag aggcggacgg caaaccgttt tttctgccgc tcaatctgct gctggggttt
    15541 atgggtgtgc atgagtggca taag
//
LOCUS       DAXFWM010000059        15365 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000059 DAXFWM010000000
VERSION     DAXFWM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.059"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..344
                     /gene="rplL"
                     /locus_tag="ACFI8B_001672"
     CDS             <1..344
                     /gene="rplL"
                     /locus_tag="ACFI8B_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC3834842.1"
                     /translation="AVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPAGAGAADAEEK
                     TEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVSKAEAEDIQKQ
                     LEAAGAKVEIK"
     gene            535..4713
                     /gene="rpoB"
                     /locus_tag="ACFI8B_001673"
     CDS             535..4713
                     /gene="rpoB"
                     /locus_tag="ACFI8B_001673"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC3834843.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(4678..4818)
                     /locus_tag="ACFI8B_001674"
     CDS             complement(4678..4818)
                     /locus_tag="ACFI8B_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834844.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            4866..9041
                     /gene="rpoC"
                     /locus_tag="ACFI8B_001675"
     CDS             4866..9041
                     /gene="rpoC"
                     /locus_tag="ACFI8B_001675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC3834845.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            9330..9743
                     /locus_tag="ACFI8B_001676"
     CDS             9330..9743
                     /locus_tag="ACFI8B_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834846.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGN"
     gene            9761..10390
                     /locus_tag="ACFI8B_001677"
     CDS             9761..10390
                     /locus_tag="ACFI8B_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding protein domain-containing
                     protein"
                     /protein_id="HFC3834847.1"
                     /translation="MSEQKIEFYGADKEQNYALLKTWVYEQPYSVVRGYFGYSRKDGN
                     PIEEDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSFQYEGRAFGGNGVLSKESLDNH
                     NGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIERIPYRESGSSLGLKDRVSYFGV
                     NEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHKHQAVIGFGGEKK"
     gene            10628..10870
                     /locus_tag="ACFI8B_001678"
     CDS             10628..10870
                     /locus_tag="ACFI8B_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834848.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            11067..11927
                     /locus_tag="ACFI8B_001679"
                     /pseudo
     CDS             11067..11927
                     /locus_tag="ACFI8B_001679"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            12106..12477
                     /gene="rpsL"
                     /locus_tag="ACFI8B_001680"
     CDS             12106..12477
                     /gene="rpsL"
                     /locus_tag="ACFI8B_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC3834849.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            12595..13065
                     /gene="rpsG"
                     /locus_tag="ACFI8B_001681"
     CDS             12595..13065
                     /gene="rpsG"
                     /locus_tag="ACFI8B_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC3834850.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13084..15189
                     /gene="fusA"
                     /locus_tag="ACFI8B_001682"
     CDS             13084..15189
                     /gene="fusA"
                     /locus_tag="ACFI8B_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC3834851.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            15277..>15365
                     /locus_tag="ACFI8B_001683"
     CDS             15277..>15365
                     /locus_tag="ACFI8B_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3834852.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTA"
BASE COUNT         4135 a         3461 c         4012 g         3757 t
ORIGIN      
        1 aagcagttgg ttctttgacc gtaatggaat tgaatgacct ggttaaagct tttgaagaaa
       61 aattcggtgt ttctgctgct gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg
      121 atgctgaaga aaaaaccgaa tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg
      181 gcgtgattaa agttgtccgt gcaattactg gtttgggtct gaaagaagct aaagacatcg
      241 ttgacggcgc acctaaaacc attaaagagg gtgtttctaa agctgaagcc gaagacatcc
      301 aaaaacaact ggaagcagca ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg
      361 aaggctggca gttttctgcc agccttattt tgcttcttaa aataagcatt aagtattgtt
      421 tacatttatt tgcatagttt ttatcaaatc attgcaaata aatgtaaata tcagattgat
      481 gcgtaccgtt gttttcagac ggcctattat tgaaaattac ttttcggagt gtgtatgaac
      541 tattcgttta ccgagaaaaa acgtatccgt aagagttttg caaagcggga aaatgttttg
      601 gaagttcctt ttctactggc aacccaaatt gattcttatg cgaaattttt gcagctggaa
      661 aatgcttttg acaaacgtac cgatgacggt ctgcaagcgg catttaattc tattttcccg
      721 attgtgagcc acaacggtta tgctcgtctg gagtttgtat attacacgct gggcgagcct
      781 ttgttcgata tccctgaatg tcaattgcgc ggaatcactt atgctgcccc tctgcgcgcg
      841 cgtatccgtt tggtgatttt ggataaggaa gcgtctaaac cgacggtaaa agaagttcgt
      901 gaaaacgaag tgtatatggg cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt
      961 aacggcacag agcgtgtgat tgtttcccag ttgcaccgtt cgcccggtgt gttcttcgag
     1021 catgacaaag gtaagacaca ctcttccggc aaattgttat tctccgcccg catcattccc
     1081 taccgtggtt catggttgga ttttgaattt gatccgaaag atttgctgta tttccgtatc
     1141 gaccgccgcc gtaaaatgcc ggtaacgatt ttgttgaagg ctttgggcta caacaatgag
     1201 caaatcttgg atattttcta cgacaaagaa acgttttatt tgtcttcaaa cggtgttcaa
     1261 accgatttgg tcgcaggccg tctgaaaggc gaaactgcca aggtcgatat cttggataaa
     1321 gaaggcaatg tattggttgc caaaggtaag cgcattactg cgaaaaatat ccgtgatatt
     1381 accaatgcag gcctgacccg tttggatgta gaacaggaaa gcctgctggg caaagcattg
     1441 gctgccgatt tgattgattc ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt
     1501 acagaagagt tgttggccaa atttgatatt aacggcgtaa aagaaattac gaccctttac
     1561 atcaatgagt tggatcaggg tgcttatatt tccaatacct tgcgcacgga tgagactgcc
     1621 ggccggcagg cggcgcgtgt tgcgatttac cgtatgatgc gtccgggcga accgcccacc
     1681 gaagaggcgg tcgagcaatt gtttaaccgc ttgttcttca gtgaagacag ctacgatttg
     1741 tcccgcgtag gccgtatgaa atttaatacg cgcacatacg aacaaaaact gtccgaagcc
     1801 caacaaaact cttggtacgg ccgcctgttg aacgagacgt ttgccggtgc tgccgacaaa
     1861 ggcggttatg tcctgagcgt cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg
     1921 cgtaacggcc atggcgaagt ggacgatatc gatcacttgg gcaaccgtcg agtacggtcg
     1981 gtaggcgagc tgactgaaaa ccaattccgc agcggtttgg ctcgtgtgga acgtgccgta
     2041 aaagaacgtt tgaatcaggc ggaatcagaa aacttgatgc cgcacgactt gattaatgcg
     2101 aaacccgttt ctgccgccat caaagaattc ttcggctcca gccaattgag tcagtttatg
     2161 gatcagacca atcccttgtc tgaagtaacc cataaacgcc gtgtatctgc attgggcccg
     2221 ggcggtttga cccgcgaacg tgccggcttc gaagtgcggg acgtgcatcc gacccactat
     2281 ggacgtgtat gtcctattga aacgcctgaa ggtccgaaca tcggtttgat caactcattg
     2341 tctgtttacg cgcgcaccaa tgattacggt ttcttggaaa cgccttaccg ccgcgttatt
     2401 gacggcaaag taaccgagga aattgattac ttgtctgcca tcgaagaagg ccgctatgtg
     2461 attgcacagg cgaatgccga tttggattca gacggcaatc tgattggcga tttggttacc
     2521 tgtcgtgaaa aaggcgaaac cattatggca acgcccgacc gcgtccaata tatggacgtg
     2581 gcaaccggtc aggtggtatc cgttgcggca tccctgattc cgttcttgga acacgatgac
     2641 gcgaaccgcg cattgatggg tgccaacatg caacgtcagg cagtgccttg tctgcgtcct
     2701 gaaaaaccga tggtcggtac cggtatcgag cgttccgttg ccgttgactc tgctactgca
     2761 atcgttgccc gccgaggcgg cgtggtcgag tatgtcgatg ccaaccgcgt tgtgatccgt
     2821 gtccatgacg acgaagcgac tgccggcgaa gtgggtgtcg atatttacaa tttggttaaa
     2881 ttcacccgtt ccaatcaatc taccaacatc aaccaacgtc cggctgtcaa agcaggcgat
     2941 gttttgcaac gcggcgattt ggtggccgac ggcgcgtcca ccgatttggg cgaattggct
     3001 ttgggtcaaa atatgaccat cgccttcatg ccgtggaacg gttacaacta cgaagactcg
     3061 attctgattt ccgaaaaagt ggctgcggac gaccgctata cttcgattca cattgaggaa
     3121 ttgaatgtcg ttgcccgcga taccaagctg ggtgcggaag acattacccg cgatattccg
     3181 aacttgtccg agcgtatgca aaaccgtttg gacgaatccg gtatcgttta catcggtgcg
     3241 gaagtagaag ccggcgatgt gttggtaggc aaggtaacgc ctaaaggcga aacccagctg
     3301 acgccggaag aaaaactgct gcgcgccatc ttcggcgaaa aagcgtctga cgtaaaagat
     3361 acttcattgc gtatgcctac cggtatgagc ggtacggtta tcgacgttca agtctttacc
     3421 cgcgaaggca tccaacgcga caaacgcgcc caatccatta tcgattccga attgaaacgc
     3481 taccgtttgg atttgaacga ccaattgcgt attttcgaca acgacgcatt cgaccgtatc
     3541 gagcgtatga ttgtcggtca gaaagccaac ggcggcccga tgaagctggc caaaggcagc
     3601 gaaatcacga ccgaatatct ggcgggtctg ccgagcaggc acgattggtt cgatatccgt
     3661 ttgaccgatg aagatttggc caagcagttg gaactgatta aattgagcct gcaacaaaaa
     3721 cgcgaagaag cggatgagtt gtatgagatc aagaagaaaa aactgaccca aggcgacgag
     3781 ctgcaacccg gcgtacaaaa aatggtgaaa gtctttatcg ccatcaaacg ccgtctgcaa
     3841 gccggtgaca aaatggcggg ccgccacggt aataaaggtg tggtatctcg cattctgcct
     3901 gtggaagaca tgccgtacat ggcggacggc cgtcctgtgg acatcgtact gaacccgttg
     3961 ggcgtacctt cccgtatgaa catcggtcag attttggaag ttcacttggg ttgggcggca
     4021 aaaggtatcg gcgagcgcat cgaccgtatg ctgaaagagc gacgcaaagc cggcgagctg
     4081 cgcgagttct tgaacaaact ctacaacggc agcggtaaga aagaagattt ggacagcctg
     4141 actgatgaag aaatcatcga attggcttcc aatctgcgta aaggtgcatc tttcgcctct
     4201 cctgtattcg acggtgcgaa agagtctgaa atccgcgaaa tgttgaactt ggcttaccca
     4261 agcgaagatc ctgaggttga gaaactgggc ttcaacgaca gtaaaactca aatcacgctg
     4321 tatgacggcc gttcaggcga agcatttgac cgcaaggtta cagtcggtgt gatgcactat
     4381 ctgaaactgc accacttggt tgacgaaaaa atgcacgcgc gttctaccgg cccgtacagt
     4441 ctggttactc aacaacctct gggcggtaaa gctcagtttg gcggccaacg tttcggtgag
     4501 atggaggttt gggcattgga agcatacggc gcggcataca cgctgcaaga gatgctgact
     4561 gtgaagtctg acgacgtgaa cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa
     4621 cacaaaatcg atgccggtat gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca
     4681 ctgggtttgg atatcgattt ggaacgctac tgatacgggt ttcagacggc ataaggggag
     4741 ctgttctgca ggtatgcggg gcagcccgac aatgtttaaa aacgaaatgc cgtctgaaag
     4801 cactgtacct ttatccatat cgaaaatccg ccacgcggta aaaatatttc cttcaaggag
     4861 cagaaatgaa tttgttgaac ttatttaatc cgttgcaaac cgcaggtatg gaagaagagt
     4921 ttgatgccat caaaatcggt attgcctctc ccgaaaccat ccgctcatgg tcttatggcg
     4981 aagttaaaaa acctgaaacc atcaactacc gtacgttcaa acccgagcgc gacggcttgt
     5041 tctgcgccaa aatctttggc ccggtcaaag actacgaatg cttgtgcgga aaatacaaac
     5101 gattgaaatt taaaggcgta acctgtgaaa aatgtggcgt agaagttacc ctgtccaaag
     5161 tgcgccgcga acgcatgggc catatcgaat tggctgcacc cgttgcccat atttggttct
     5221 tgaaatccct gccttcccgc ttgggtatgg tattgaacat gacgttgcgt gatatcgaac
     5281 gcgtattgta ctttgaagca ttcgtggtga ccgatcccgg catgactccg ttgcaacgtc
     5341 gtcaattgct gactgaagac gattactaca acaaactgga cgaatacggc gacgacttcg
     5401 atgccaaaat gggtgcggaa ggtatccgcg aattgttgcg aaccttggat gtagcaggcg
     5461 aaatcgaaat cctgcgccaa gagcttgaat cgaccggttc tgacaccaaa atcaaaaaaa
     5521 tcgctaaacg cttgaaagta ttggaagcct tccatcgttc cggtatgaag ctggagtgga
     5581 tgattatgga tgtgctgccg gtattgccgc ctgatttgcg tccgttggtt ccgttggacg
     5641 gcggccgttt tgccacttcc gatttgaacg atttgtaccg ccgcgtcatc aaccgcaaca
     5701 accgtctgaa acgcctgctg gaactgcatg cgcctgacat catcgtccgc aacgaaaaac
     5761 gtatgttgca agaagcggtt gactcgctgt tggataacgg ccgtcgcggt aaagccatga
     5821 ccggagccaa caaacgtccg ctgaaatcgt tggcagatat gattaaaggt aaaggcggcc
     5881 gcttccgtca aaacctgttg ggcaaacgtg tggactactc cggccgttcc gtgattaccg
     5941 taggcccata cctgcgtctg caccaatgcg gtttgccgaa aaaaatggcg ttggaactgt
     6001 tcaaaccatt tattttccac aaattggaaa aacaaggttt ggcgtctacc gttaaagctg
     6061 cgaaaaaatt ggtagaacaa gaagtacctg aagtatggga tatcttggaa gaagtcatcc
     6121 gcgaacatcc gattatgttg aaccgtgcgc cgaccctgca ccgtttgggt attcaagcgt
     6181 tcgagcctat tctgatcgaa ggtaaagcga ttcagttgca cccattggtg tgtgccgcat
     6241 ttaatgccga ctttgacggt gaccaaatgg cagtacacgt tccattgagc ttggaagcgc
     6301 aaatggaagc acgcacgctg atgctggctt caaacaacgt attgtctcct gccaacggcg
     6361 aaccgattat cgtaccttcc caagacatcg tattgggtct gtactacatg acccgcgacc
     6421 gtatcaatgc caaaggcgag ggcagcctgt ttgccgatgt gaaagaagtg catcgtgcat
     6481 accataccaa acaggtcgag ctgggtacaa aaatcaccgt acgtctgcgc gaatgggtga
     6541 aaaacgaagc aggtgagttt gagcctgtcg ttaaccgtta cgaaacaacc gtcggccgtg
     6601 cattgttgag cgaaatcctg ccgaaaggcc tgccgtttga atatgtcaac aaagcgttga
     6661 agaaaaaaga aatttccaag ctgattaacg catcgttccg cctgtgcggc ttgcgcgata
     6721 cggttatctt cgccgaccac ctgatgtaca ccggtttcgg atttgcggca aaaggcggta
     6781 tttccattgc cgttgacgat atggaaattc caaaagaaaa agcggccttg ctggctgaag
     6841 ccaatgccga agttaaagaa atcgaagacc aataccgtca aggtttggtt accaacggcg
     6901 aacgctacaa caaagtggtc gatatttggg gtcgtgccgg cgataagatt gctaaagcga
     6961 tgatggacaa cctgtccaaa caaaaagtta tcgaccgtga cggcaacgaa gtcgatcaag
     7021 agtcattcaa ctccatttat atgatggcgg actccggtgc ccgtggttct gcggctcaga
     7081 ttaaacagtt gtccggtatg cgcggtttga tggcgaaacc tgacggatcg attattgaaa
     7141 cgccgattac ctcaaacttc cgcgaaggtc tgaccgtatt gcaatacttt attgcgaccc
     7201 acggtgcgcg taagggtttg gcggataccg cgttgaaaac cgcgaactcc ggttacctga
     7261 cccgtcgtct ggtagacgta actcaagact tggtcgttgt tgaagacgat tgcggtactt
     7321 cagacggctt tgtcatgaag gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg
     7381 atcgtatttt gggtcgtgtt accgcgtctg acgttgtcga tccgtcaagc ggcgagactt
     7441 tggttgaagc cggtacgttg ttgactgaaa aactggtgga tatgattgac caatccggtg
     7501 tcgatgaagt caaagtccgt actccgatta cttgtaaaac ccgccacggt ctgtgcgcgc
     7561 actgttacgg ccgcgacttg gcgcgcggca aactggtcaa tgccggcgag gcagtcggcg
     7621 tgattgcggc tcagtctatt ggtgaaccgg gtacccagtt gaccatgcgt acgttccaca
     7681 tcggtggtgc ggcatcccgt gcggcagcag ccagccaagt cgaagccaaa tccaacggta
     7741 cggcacgttt cagcagccaa atgcgttatg ttgccaacaa caaaggcgag ttggttgtca
     7801 tcggccgttc ttgcgaagtc gtgattcacg atgacatcgg ccgtgaacgc gaacgccaca
     7861 aagtacctta cggtgccatc ctgctggtac aagacggtat ggccattaaa gccggtcaaa
     7921 ccttggcaac ttgggatccg catacccgtc cgatgattac cgaacatgca ggtatggtga
     7981 aattcgagaa catggaagag ggcgttaccg ttgcaaaaca aaccgatgat gtaaccggtt
     8041 tgtccgcttt ggtagtgatt gacggtaaac gccgttcctc tagtgcttcc aaactgctgc
     8101 gtccgactgt gaaactcttg gacgaaaacg gcgtggaaat ctgtattccc ggtacgtcca
     8161 ctccggtttc gatggcattc cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa
     8221 tcggtaaagg cgacgtattg gcgcgtattc cgcaagcctc ttccaaaacc cgcgacatta
     8281 ccggcggtct gccgcgcgtt gccgagctgt ttgaagcacg cgtgccgaaa gatgccggta
     8341 tgctggcgga aattaccggt accgtttcct tcggtaagga gaccaaaggc aaacaacgtc
     8401 tgattatcac tgacgtggac ggtgtagcat acgagacttt gatttccaaa gagaaacaaa
     8461 ttctggtaca tgacggtcaa gtggtaaacc gaggcgaaac cattgtggac ggcgcggtcg
     8521 atccgcacga tattctgcgt ctgcaaggta tcgaagcact ggcacgctac attgtccaag
     8581 aggtgcaaga ggtttaccgc ctgcaaggtg tgaagatttc cgataaacac atcgaagtca
     8641 tcatccgtca aatgttgcgc cgtgtgaaca ttgcggatgc cggcgaaacc gggtttatta
     8701 ccggagagca ggtcgaacgc ggcgatatga tggcggccaa tgaaaaagct ttggaagaag
     8761 gcaaagagcc ggcacgttac gaaaacatat tgctgggtat taccaaagcc tccctgtcca
     8821 ccgacagctt catttctgcc gcatcgttcc aagaaaccac ccgtgtgttg accgaagctg
     8881 ccatcatggg caaacaagac gaactgcgcg gtctgaaaga gaatgtcatc gtcggtcgcc
     8941 tgattcctgc cggtaccggt ttgacttatc accgcagccg tcatcagcaa tggcaagggg
     9001 tagagcagga gactgctgaa acccaagtaa cggatgaata atcttcggcg cgttcattca
     9061 ataaaaaacc gcaagccttg agcttgcggt ttttctttgt ccgattaagg caaaaacaag
     9121 cgttttcgtc attttgaggc gtgtggatat aagcccgata agtgtcccat atcttcgtgc
     9181 cgtcaaatta tgcatcgaat agaacgagtt aaatccgaag gttattgtgt taaatattgc
     9241 aaaaatactt taaaaatttc gcacggtggg ggggtagaat tcccagactt aaagcttaag
     9301 tttttttaac aaagtttggg ggagtctaaa tgaaagtatc tcaatcagca atgaataaac
     9361 ttgttattgc tgttattgct tctatggggc taacagcgtg cgcgggtggc ggttccgatt
     9421 catctatgtc ggttcaacca agcgtttcgg aacaactgaa agacaatgca aatgttgatg
     9481 cgaaagatga aaaagttatt gaatatctga agaaatcatc attaaaagat gtaccaaaag
     9541 aattgcaagc caaagtatta aaggttaaag gtgatgaata tacaggagtg cgaaaacaat
     9601 atgcaggtaa gctcggtaag ggtgaatctg tcaaggcaat gctatttcta gacggcgaag
     9661 agccgtttag taaggaacaa cttcagaaga tggatgtata tgttaatggt aaaaaatacg
     9721 aaggtagtaa gggggggaac tagatgttct ccccaaaggt ttgagtgagc aaaagattga
     9781 gttttacggt gcggataaag aacagaatta tgcgctatta aaaacttggg tttatgagca
     9841 accgtattct gtggttagag gctattttgg ctacagtcga aaagacggta atcctataga
     9901 agaggatgga caaaatcctg aagagattcc ttttgacttg tatctgggcg acattagagg
     9961 cgtggcaaca gatgaagaca aattacccaa ggctgggagc tttcaatatg aaggtcgggc
    10021 atttggcggt aatggggttt tatccaaaga atcgttagat aaccataatg gcgtatttag
    10081 atacacaatt gattttgatc gtagaaaagg ctctggctca attgaaggaa tggaacaata
    10141 tggcaagatc aaattagaag aagctgctat tgagagaatc ccttatcgag agtccggctc
    10201 cagcttgggg ttgaaagacc gggtttctta tttcggcgtg aacgaagggg tggctatgtt
    10261 agagaaggat aatgagatta aaaagtatca tttgggtatt ttcggcgaag cggctaatga
    10321 agtagcaggt gctgtaagcc aagagcataa acatcaagct gttatcggtt tcggcggcga
    10381 gaagaaatag agaaagcact atttctattt agtgccgttt gtccgggcaa gatgccgtct
    10441 gaagtttcag acggcatttt cttcaaaaaa cagtccgcca aatcgaatac tgccaagcgg
    10501 caatgcaata cctcaaaaca gcaataattt cttttaaaac agttgattct tactgaacat
    10561 aaggccgggc aatacgaaaa gaaaaatggc gttgctgggc attttatttg aatttgttat
    10621 gaaattgatg aaatctaaaa atatcttttt tatgggatac ctaatcgctt cggcgacgtt
    10681 tgccgaggat atcggcgtgc ctgtcgaacc gattaacgtc ggtagtcggg ctgcgatgcc
    10741 gtctgaaggg gaaagcctcg ccctcctgcc gtttgcagag gatgtgccgc cggttcgcga
    10801 tgcaatgccg tctgaagttc ctaaaagcgc ggcaggcggc gatgttcgag gcggccgatc
    10861 tataccgtga gttgttgtcg gaaagacccg atcttgttta tccccgtttt gatttgggcg
    10921 tgatgctgtt tgaagacaaa caatatcgtg aggcattggt tcagttgcac cgggccgagg
    10981 aggtgttgcc gcccgatatg cggcagcttg cacgggaata tatccgtcag gcggaagcgg
    11041 tgcaggcgtg gcatccttcg ttcaatatga actatgaaca aacagataat gtaaacaatg
    11101 cgtccctttc acgggacatc gtcatcaacg gccggaaatg gataaaatcg gaagacagcc
    11161 tgcccaaacg cgccaacggg ataaggtacg aactgggcgt tgatcggatg tttaacatgg
    11221 caggcaatca ctttgcccgc ttaggcatca gcggttccgg tgtgcattat tggaatgccc
    11281 gggattttag cgaacaggcg tttcatgccg aagtaggcta tcgttatcgg aatagccgtc
    11341 tagagtgggg attccggccc tttgtcaaac aaaaccggct gggcaataac cgttatacgg
    11401 caaatacggg tattgtgttg gattacagcc gccgtctgaa tgaaaaatgg cgcagcaccc
    11461 aatctttcca gtatggccgc aaacaatacc atgacgaata tttggcaaaa cgttacaaca
    11521 gtaaaacgat ttcggtgagc ggtacgttca gctattacgc tatgtcggct tggcagttgt
    11581 acgggggtat tagcggtatg tttgataata ctgtggagaa agagcaggct tcacggcgtt
    11641 atggtgtaag tttgggtacg gtgaaaatat tagacggcgg tcttggactg aagttgggtg
    11701 cgggttacac gaaacggata tttaaagccc ctgcaacatt aatttacaat tttactcggc
    11761 gtgatgatta acatcggatg agcgcggctt tatggcataa aaaactgtcg tggaaaggat
    11821 ttacacccca aataaatttc cgttacaaca agaacaacag caatatgccc gccttttatt
    11881 cgcacagcgg caagggatgg tttgtcagta tggaaaaaac gtattgacag tattttctcc
    11941 agccgtccga ctgattgtga gggatgtcgg taaatattta tcggcaaaca agaaaatcat
    12001 cttttttctt gtcgttatgc ttgactgtct gcttgcaata aaaatataat tccgctcttg
    12061 ccgacatggt gtcggcaagt atttaactca acaggacgag aaaatatgcc aactatcaac
    12121 caattggtac gcaaaggccg tcaaaagccc gtgtacgtaa acaaagtgcc cgcactggaa
    12181 gcctgcccgc aaaaacgcgg cgtgtgcacc cgtgtataca cgactacccc taaaaaacct
    12241 aactctgcat tgcgtaaagt atgtaaagtc cgcctgacca acggttttga agtcatttca
    12301 tatatcggcg gtgaaggcca caacctgcaa gagcacagcg tcgtactgat tcgcggcggc
    12361 cgtgtaaaag acttgccggg tgtacgttac cacactgtac gcggttcttt ggatactgca
    12421 ggtgttaaag accgcaaaca agcccgttct aaatacggtg ctaagcgtcc taaataatta
    12481 ccgggactca aataggcacg tcggccgcct aagctgaaca acggccgagt aagtgaatac
    12541 tcaattgggt attcatggga atagacccga ctgaatagat taaaggaaat taaaatgcca
    12601 agacgtagag aagtccccaa gcgcgacgta ctgccagatc ctaaattcgg tagcgtcgag
    12661 ttgaccaaat tcatgaacgt attgatgatt gacggtaaaa aatccgttgc cgagcgtatc
    12721 gtttacggtg cgttggaaca gattgagaaa aaaaccggca aagcagcaat cgaagtattt
    12781 aacgaagcca ttgcaaactc caaacctatc gtggaagtga aaagccgccg tgtaggtggt
    12841 gcaaactacc aagttcctgt tgaagttcgt ccttcacgcc gtctggcttt ggcaatgcgt
    12901 tgggttcgcg acgcggcccg caaacgtggt gagaaatcca tggatctgcg tttggcaggc
    12961 gagttgattg atgcgtccga aggccgtggc ggtgcgttga aaaaacgtga agaagtacac
    13021 cgtatggctg aagccaacaa agcattctct cacttccgtt tctaattttg aaaggctaat
    13081 aaaatggctc gtaagacccc gatcagcctg taccgcaaca tcggtatttc cgcccatatc
    13141 gatgcgggta aaaccacgac gacagaacgt attttgttct ataccggttt gacccacaag
    13201 ctgggcgaag tgcatgacgg tgcggctact accgactaca tggaacaaga gcaagagcgc
    13261 ggtattacca ttacctccgc tgccgttact tcctactggt ccggtatggc gaaacaattc
    13321 cccgagcacc gcttcaacat catcgacacc ccggggcacg ttgactttac cgtagaggta
    13381 gagcgttcta tgcgtgtatt ggacggcgcg gtaatggttt actgtgcggt gggcggtgtt
    13441 caaccgcaat ctgaaaccgt atggcggcaa gccaacaaat accaagttcc gcgcttggcg
    13501 tttgtcaata aaatggaccg ccaaggtgcc aacttcttcc gcgttgtcga gcaaatgaaa
    13561 acccgtttgc gcgcaaaccc cgtacctatc gtcattccgg taggcgcgga agacagtttc
    13621 accggtgttg tcgatttgct gaaaatgaaa tctatcatct ggaatgaagc cgataaaggt
    13681 acaaccttta cctatggcga tattcctgcc gaattggtcg aaactgccga agaatggcgt
    13741 caaaatatga ttgaagccgc agccgaagcc agcgaagaac tgatggacaa atacttgggc
    13801 ggtgaagatc tggccgaaga agaaatcgta ggcgcgttgc gtcaacgtac tttggcaggc
    13861 gaaattcagc ctatgctgtg cggttctgca tttaaaaaca aaggtgttca acgtatgttg
    13921 gacgcagttg tagaattgct gccagctcct accgatattc ctccggttca aggtgttaat
    13981 cctaacactg aagaagccga cagccgtcaa gccagcgatg aagagaaatt ctctgcattg
    14041 gcattcaaaa tgttgaacga caaatacgtc ggtcagctga cctttatccg cgtttactca
    14101 ggcgtagtaa aatccggcga taccgtactg aattctgtaa aaggcactcg cgaacgtatc
    14161 ggtcgtttgg tgcaaatgac tgccgcagac cgtactgaaa tcgaagaagt acgcgctggc
    14221 gacatcgcag ccgctatcgg tctgaaagac gttactaccg gtgaaacctt gtgtgcggaa
    14281 agcgcgccga ttatcttgga acgtatggaa ttccccgagc cggtaatcca tattgccgtt
    14341 gagccgaaaa ccaaagccga ccaagagaaa atgggtatcg ccctgaaccg cttggctaaa
    14401 gaagaccctt ctttccgcgt tcgtacagac gaagaatccg gtcaaaccat tatttccggt
    14461 atgggtgagc tgcacttgga aattattgtt gaccgtatga aacgcgaatt cggtgtggaa
    14521 gcaaatatcg gtgcacctca agtggcttac cgtgaaacta tccgcaaagc cgttaaagct
    14581 gaatacaaac atgcaaaaca atccggtggt aaaggtcaat acggtcacgt tgtgattgaa
    14641 atggaaccta tggaaccggg tggtgaaggt tacgagttta tcgatgaaat taaaggtggt
    14701 gtgattcctc gcgaatttat tccgtctgtc gataaaggta tccgcgatac gttgcctaac
    14761 ggtatcgttg ccggctatcc tgtagttgac gtacgtatcc gtctggtatt cggttcttac
    14821 catgatgtcg actcttccca attggcattt gaattggctg cttctcaagc gtttaaagaa
    14881 ggtatgcgtc aagcatctcc tgccctgctt gagccgatta tggcagttga agtggaaact
    14941 ccggaagaat acatgggcga cgtaatgggc gacttgaacc gccgtcgcgg tgttgtattg
    15001 ggtatggatg atgacggtat cggcggtaaa aaagtccgtg ccgaagtacc tctggcagaa
    15061 atgttcggtt actcgaccga cctgcgttct gcaacccaag gccgcgctac ttactctatg
    15121 gagttcaaga aatattctga agctcctgcc cacatagctg ctgctgtaac tgaagcccgt
    15181 aaaggctaat caggcaaata ggccgtctga aaggctgaaa tgatttttca gatggcattg
    15241 ttctttaatc gatctttaat gtaaaggaat tagctcatgg ctaaggaaaa attcgaacgt
    15301 agcaaaccgc acgtaaacgt tggcaccatc ggtcacgttg accatggtaa aaccaccctg
    15361 actgc
//
LOCUS       DAXFWM010000060        15287 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000060 DAXFWM010000000
VERSION     DAXFWM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.060"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1408..2517
                     /gene="fic"
                     /locus_tag="ACFI8B_001684"
     CDS             1408..2517
                     /gene="fic"
                     /locus_tag="ACFI8B_001684"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC3834853.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(3245..4057)
                     /gene="murI"
                     /locus_tag="ACFI8B_001685"
     CDS             complement(3245..4057)
                     /gene="murI"
                     /locus_tag="ACFI8B_001685"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC3834854.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            4246..4707
                     /gene="tsaE"
                     /locus_tag="ACFI8B_001686"
     CDS             4246..4707
                     /gene="tsaE"
                     /locus_tag="ACFI8B_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC3834855.1"
                     /translation="MSDFSPVPRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4704..5954
                     /locus_tag="ACFI8B_001687"
     CDS             4704..5954
                     /locus_tag="ACFI8B_001687"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC3834856.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADPAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            6036..6854
                     /locus_tag="ACFI8B_001688"
     CDS             6036..6854
                     /locus_tag="ACFI8B_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC3834857.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6900..8042)
                     /locus_tag="ACFI8B_001689"
     CDS             complement(6900..8042)
                     /locus_tag="ACFI8B_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC3834858.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVAGAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(8091..8513)
                     /locus_tag="ACFI8B_001690"
     CDS             complement(8091..8513)
                     /locus_tag="ACFI8B_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834859.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(8506..9315)
                     /locus_tag="ACFI8B_001691"
     CDS             complement(8506..9315)
                     /locus_tag="ACFI8B_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC3834860.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVAVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(9397..9774)
                     /gene="acpS"
                     /locus_tag="ACFI8B_001692"
     CDS             complement(9397..9774)
                     /gene="acpS"
                     /locus_tag="ACFI8B_001692"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC3834861.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFCNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9824..10552)
                     /gene="pdxJ"
                     /locus_tag="ACFI8B_001693"
     CDS             complement(9824..10552)
                     /gene="pdxJ"
                     /locus_tag="ACFI8B_001693"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC3834862.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFAKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAQ
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(10605..11348)
                     /gene="recO"
                     /locus_tag="ACFI8B_001694"
     CDS             complement(10605..11348)
                     /gene="recO"
                     /locus_tag="ACFI8B_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC3834863.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(11385..12512)
                     /gene="pheA"
                     /locus_tag="ACFI8B_001695"
     CDS             complement(11385..12512)
                     /gene="pheA"
                     /locus_tag="ACFI8B_001695"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC3834864.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(12571..13778)
                     /locus_tag="ACFI8B_001696"
                     /pseudo
     CDS             complement(12571..13778)
                     /locus_tag="ACFI8B_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768606.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(13894..14769)
                     /locus_tag="ACFI8B_001697"
     CDS             complement(13894..14769)
                     /locus_tag="ACFI8B_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC3834865.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
     gene            14952..15287
                     /locus_tag="ACFI8B_001698"
                     /pseudo
     CDS             14952..15287
                     /locus_tag="ACFI8B_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         3542 a         4371 c         4091 g         3283 t
ORIGIN      
        1 gacctgaaat caacggtaaa tccaagttgc ttttcaaacc gcctgcaacc gcaaaatctg
       61 ccgcaatccg ctccctcccc cgtgggggag ggccggggag agggcattct ccgagttgcg
      121 gcaacctttc ccaaactccc ttagtcttct aaaatacaaa ccttgcggct tgttgccctc
      181 tctctaactc tctcccgcag ggagagagga cggggtggct gttggggtta aggtttatgc
      241 aaactaatac ggcttgctgc cctctctcta gctttctccc acagggagag aggacggggt
      301 ggctgttggg gttaaggttt atgcaaacta atacggcttg tgcaggctgt tttttatttt
      361 tcatacgacc tgaaatcaac ggtaaatcca agttgctttt caaaccgcct gcaaccgcaa
      421 aatctgccgc aatccgctcc ctcccctgcg cgggggaggg ccggggagag ggcattctcc
      481 gagttgcggc aatctttccc aaactccctt agtcttctaa aatacaaacc ttgcggcttg
      541 ttgccctctc tctaactctc tcccgcaggg agagaggacg gggtggctgt tggggttaag
      601 gtttatgcaa actaatacgg cttgctgccc tctctctaac tctctcccgc agggagagag
      661 gactatgatg cccaccggcg gcttgctatt aggaatatct gaatcgtcat tcccacgaaa
      721 gtgggaatct agaacgtcaa atctcaagaa accgttttat ccgataagtt tctgttccga
      781 caggtccgga ttcccgcctg cgcgggaatg acgaatccat ccatacggaa acctgcatcc
      841 cgtcattccc acgaacctac attccgtcat tcccgcgaaa gtgggaatct agttttttga
      901 gtttcagtca ttcccgataa attgccttag cattgaaggt ccggattccc gcctgcgcgg
      961 gaatgacggg atttgaggtt tctgttttta attttctgtt ttcacgggaa tgacggttta
     1021 aaagttaccc gaaacctcaa aaaaaagctg tgttttaata tagtgttgat agacgtactt
     1081 ggcctccatg tattcaatcg tggaaatcta tatcttcgtc ctcgccgaaa tagtctatgc
     1141 ccgatataca attttgatac acaaacttgg aaatatgggt atcgtcgccg gagcgataga
     1201 atgcggacag tttatatata tacggttttt ttaggggagc gggcagatga agggcggatt
     1261 ttgaattaac cccatgcaaa ttgacttttg cgggcggttt taccgcccat atatttacaa
     1321 aagccaaatt tttaaacata tatccttgat atatacacgg cgtaaacata tactggaaac
     1381 atctttaaat tttccgaaat tttaaacatg agcaactgga aacccgatat tccctataac
     1441 gatttaccac ccctgccgcc aaaacaggat attgaaagca aaaccatcct gaaacgttgt
     1501 atagccgccc gtgcatccct tgcccgttta aagcaggcgg cagaattgat accgaatcaa
     1561 gccatgctga ttaacaccct tcctgttatg gaagcccgtg caagttcgga aattgaaaac
     1621 atcgtaacca ccacggacaa gctgtttcaa tccctgcaaa tggatacgga acggcaagac
     1681 cctgccacga aagaagccct gcaataccgc accgccctgt ttgcaggcta tgaatcactg
     1741 gcgagccgcc ctttatgcac acaaaccgcc atcatggtct gcaacgccat caagcacccc
     1801 tacgaaacgg ccatccgcaa aacaggcggc acagccctaa aaggaggtaa cagcggaaat
     1861 gttgtctata ccccgcccga aggagaagaa accatacgcg gcaagctggc aaattgggag
     1921 cggtttattc acgaaagcgg cgatttagac ccgcttgtca tcatggcggc ggcacattac
     1981 caatttgaag ccatccaccc gtttacggac ggcaacgggc ggacgggacg catattgaac
     2041 agcctgctat tgattgaaaa agggcttttg gatttgccta ttttgtattt gagccgctac
     2101 atcatcgaaa acagggcgga ctattaccgc ctgcttttag gcgtaaccga acggcaggat
     2161 tgggaaagct ggataatcta catcttagac ggcgtagccg acaccgccga ttggacggta
     2221 tcgaaaatag atgcgatacg ctgcctgttc gagcagacac gtcaacacat acggacacac
     2281 gcacaaggaa tctacacgca cgaactggta aatcttctgt ttgagcagcc atatacacgc
     2341 attgccaacc tagaagcggc agggatagcc aaacggcaga cggcctctaa gtacctgaaa
     2401 gagctttcag acataggtgt gctgcaagaa atcgccatcg gcagggacaa actattcatt
     2461 catccgcgcc taatggaact attgcgggga gagggcaaca gctttacttc attctaaccc
     2521 cctcttcatc cctacatgac taacacgaaa cagggatttt gacacccgaa ccggtacccc
     2581 ttgtattttc cccgcgaaaa gccggcatcc gcccgcgcat catgggagca acaaaacccc
     2641 tgcctaaaat tttgacttgt gcaaattagg ggtatatttg ggggtatatt gaaaaatggc
     2701 taaaataaaa tgtttaataa acaaaatgtt gaaacttaat ttcgatagag cctctgcata
     2761 tcgtattgag gcgttaatgg aatttgagaa agctattttt aaataagaaa aggtaactat
     2821 taaatagcta cctttctaaa attaaatatc aacacatcgt taaaacacag ccaaaaaaaa
     2881 accgaaaccg aacggtccgg attcccgcct gcgcgggaat gacggatttc aagattacgg
     2941 tattgtcggg gatgatggga aacggtggga attgtgtaaa aaatgccgtc tgaagccttt
     3001 caggcggcat ttgcggcatt cggacattta aaaatcccgt cattcccgcg caggagggaa
     3061 tccgaataca tccgcacaga aacctgcatc ccgtcattcc cgcgaaagcg ggaatctaga
     3121 acgtaaaatc taaagaaacc gttttatccg acaagtttcc gcaccgacag gtctagattc
     3181 ccactttcgc gggaatgacg ggattttagg tctctgtaat ctcgggcagc tttccgttat
     3241 cgttttaacc caaagacacc atttcaatct gctccatcgt cctgcccaga aaacgctcgc
     3301 cgatggttct gaatttcaaa ggaatatcgc tgacgtaaaa acggtagtcg ggattgttgt
     3361 tgccggtatc gagcaatcct tcctgtgcaa ggacgcgtgc ggtttcttcg gccgtcgtaa
     3421 tcgcggaatc gaccagcgcg acattgtgcg cctccctgcc gattaagggc ttgagcaagg
     3481 gaaagtgcgt gcagcccaac accagcgtat cgatgccgtc tgcaagcagt ggtttgaggt
     3541 attcgcatac ggtcaggcgg gtaacttcgt gttccaacca accttcctcc accaaaggga
     3601 cgagcagcgg cgcggcctgc gtgcggacga gcgtgtcggg gttgtccctg tggatggcgc
     3661 gcgcataagc attgctgttg actgtcgtat tggtggcgat gatgccgatt ttattgttgc
     3721 gcgtcgttgc cagcgcggct ttggcgccgg cggaaatcac gtccaaaacg ggcatattgc
     3781 ccgtcttttg acggattttc cgccccgcaa ccgccgcaat cgtgttgcac gcgataacca
     3841 tcgccttgac atcgtgtccc aataaaaaat cgacaatctg catcgagaaa ttttcgatgg
     3901 tcgccttaga tttcgtcccg taaggcacgc gcgccgtgtc gccgaaataa atgatgttct
     3961 ccatcggcag ccgttccatc agcgcgcgca cattggtcaa accgccgatt cccgagtcaa
     4021 aaacgccgat gggccgctgc ctgccgatat tttccatcct ttttccaatc cgtcctaaac
     4081 atacaatccg catattgtac acgcgccgct ttttcttgac agccatgccg tctgaaagca
     4141 ggatgcgtat ttggctgtta taatgtacgg caatgccgcc gccgggcgca cggtttgccg
     4201 accgcctgcc cctccgatcc gccacataaa agttgtaaag cccctatgtc tgatttttcc
     4261 cccgttcccc gattccttgc cgacgaagcc gccacactcg atttgggcgc ggcgtggtct
     4321 tcccgtttaa acgcaccgct ggtcatttac ctcgaagggg atttgggtgc gggcaaaacc
     4381 acgctgacgc gcggcatcct gcgcggattg ggtcaccagg gcgcagtcaa aagcccgacc
     4441 tacgccatcg tcgaatctta tccgctggaa cgcttcgccc tgcaccattt cgacctctac
     4501 cgcttctcgt tccccgaaga atgggaagac gcggggcttg acgagctgtt tgccgcaaac
     4561 agcgtctgcc tgatcgaatg gccgcaacag ggaggcgaat ttaccccgcc tgccgacatc
     4621 accgcaacat tgacacacgg cggcggcggc agaaaatgcc tgctgaccgc ccataccgaa
     4681 cgaggacgcg aaagcctgcc gctatgacca aactgacacg aagacaaatc atccgccgca
     4741 ccgccggcac actgttcgcc ctaagcccca tcgcatccgc cgttgccaaa acggtacgcg
     4801 ccccgcagtt taccgccgca cggatatggc cgtcgcacac ctacacccgc ctgacgctgg
     4861 aaagcaccgc cgcgctcaaa taccagcact tcgcgctcga caacccgggc aggttggtcg
     4921 tcgatataca aaacgcaaac atcaataccg tattgcacgg gctttcccaa aaagttatgg
     4981 cggacgaccc ctttatccgc agcatacgcg cgggtcagaa cacgccgacc accgtccgcc
     5041 tcgtcatcga cctgaaacag cccacccacg cacaagtctt cgcgcttccg cccgtcggcg
     5101 gctttaagga ccgcctcgtc gtcgacctct atccgcacgg gatggatgcc gacgatccga
     5161 tgatggcgct gctcaacggc agcctgaaca aaaccctgcg cggctctccg gaagccgacc
     5221 ccgcccaaaa caccacgccc cgacccgggc ggggcaaaaa cgggcgcaga cccgtcatca
     5281 tgctcgatcc gggacacggc ggtgaagacc ccggcgccgt cagcccgggc ggtttgcagg
     5341 aaaaacacgt cgtcctctcc atcgcccgcg aaaccaaaaa acaattggaa gccttgggtt
     5401 ataacgtctt tatgacgcgc aacgaagacg tgttcatccc attgggcgtg cgtgtcgcca
     5461 aagggcgcgc acggcgggcg gacgtattcg tctccatcca cgccgacgct ttcaccagcc
     5521 cctccgcgcg cggaacgggg gtttatatgc tgaacaccaa aggcgcgacc agctctgccg
     5581 ccaaattctt ggaacaaacc caaaataatg ccgacgcggt cggcggcgta ccgaccagcg
     5641 gcaaccgcaa cgtcgatacc gccctgctcg acatgacgca aaccgccaca ctgcgcgaca
     5701 gccgcaaact cggcaaactg gtgcttgaag aattgggcag gctcaaccat ctgcacaaag
     5761 gcagggtgga cgaagccaat ttcgccgttt tgcgcgcacc cgatatgccg tctatcctgg
     5821 tcgaaaccgc cttcctgtcc aatcctgccg aagagaagct gctgggcagc gaatccttcc
     5881 gtcggcagtg cgcccaatcc attgcctcgg gtgtccaacg ctacatcaat acatccgtat
     5941 tgaagcgggg ttgattgaaa aggcggtgtt tcaaaatata gttgtcggat gaaggcaggc
     6001 ataaacctac ttccgcccca aaccgcgttt ttatgatgaa aggtatggat aaattgagat
     6061 accaacggga ttttttaaat atccgcccca tttttacagc cggcgaacaa gaatacctga
     6121 ccgaattgtc cgatcggctt cctttatctg ttttaacgga ctccgtgcgt aatattgaag
     6181 aaatcggtat cgattttgtg tacagttctg ccaaattgga aggaaacact tataatcaat
     6241 acgatacgca ggcattgctt aaactcgggc aaaccgcagg cgggaagtta tattcggatg
     6301 ccgtaatgtt aatcaacctg agggaaagtt accggcacct gctgtccggt ttggacagcc
     6361 ccaagccgtt tgactggttg gatttcttaa aaaccaccca cagcctgatt tcggaaaact
     6421 tactggaaaa aggctccggc ggcgtagtcc gccgcgacag cgttactata agcgggacag
     6481 actacacccc tttatccaac ccgcaaagtt tggatacgga attgaaatgg ttgcttcaag
     6541 aagcccccaa aatagaaaat cctttcgacc gcgccgttta tctccataat aatttggcgt
     6601 atctccgata ttttaaggac tgcaataaac gtactgccag aaactgtatg accttgtcgc
     6661 tgatgcgctc cggttttttt ccttgcgtat tctcccccga cagttatccc gcctatgccg
     6721 aggctgtcgt agcctattac gaaaccggcg attacggttt attcaagaaa tattttattt
     6781 cggcatacga aaataccgta aataagtacg gtccccagcc tgatgtggat attttccgaa
     6841 atttctccat ttaaaaagcc gtctgaacat ttttcagacg gcttttttgt acactttcct
     6901 caacggttcg atccctgcac catatcgatc aggaacaggc ggcagtcgag gttgccgtta
     6961 taaagcggga ttttccgctt gggcagcagg cgcatgaatt tgggcatttc cctgtcgccg
     7021 gtaaacatgc cgacccgcca gccggcatag tgttttttca accacgtccc caactgcgga
     7081 taaagtgcct gcaaagcgcg gacttcctca aggcgcacgc cgtagggcgg attggacacc
     7141 atgatgccgt tttcgccgtt cggacggacg gactgcgcgc cggcaacgct gaaggaaacg
     7201 atgtcgtccg ctccggcatt gtgtgcgttg tccaatgccg tctgaacgat gcggcggccg
     7261 ttgtcgctgc ctgcaatcgg ggcgcggacg gggcgggttt gcgcttcggc gcggcggcgc
     7321 aaatccgccc acagcgtttt atcgaaattt tgcagttttt caaaaccgaa gcggcgcatc
     7381 atacccggcg cgcggccggc ggcaatccaa gcggcttcga tagcaatcgt gccgctgccg
     7441 caaaacgggt cttgaaacgg ctgcgtgccg tcgtagcctg ccgagagcag cagcccggcg
     7501 gcaaggtttt cgcgcagcgg ggcttcgccg gtatccaggc ggtagccgcg tttgaacagg
     7561 gcttcgcccg aagtgtcgat gaagatttcg acattgcgtt cgtccaaaaa ggcgtggatg
     7621 cggacatcgg gcgcggcttt gtccacgctc ggacgtgcgt cgtaaatgtc gcggaaagcg
     7681 tcgcagacgg catctttgac ggtcaggccg gcaaattgga ggcttttgac gttggcgcgt
     7741 tttgcctcga ttttgacttt gaacgtctgc tgcaaggtaa accagttgaa ccagttgatg
     7801 tttttggcaa gtttgtagat gtcgcgctcg gttcggtatg tgcctttggt caggcgcagc
     7861 aggatgcggc cggcggtacg cgagtgcagg ttggcggcgt aggcctgttc caatccgccc
     7921 cggcaggaaa cgccgccgtc aaacgcttgg acatcggtac agccgaggct tccgagttct
     7981 tgagataaaa cggtttccaa gccgcgcggg caggtggcga aaagtgtata taaagtattc
     8041 atagcgtgtc ctttccggac ggatgttccg gcaatggcgg attgaaaggt ttattcggcg
     8101 gtaaatgccg tctgaaatgt ccggctgccg attgtaatgt ccgccgtaaa atcgcgcctg
     8161 ccttcgacac agacgggcag gcggatgcgt gctgcctgcc aagtccccga cggttgccgc
     8221 tcgaacatat agcggttgaa acccatatcc atatttttca tactgaagct gatgctgacc
     8281 tgttccgtgc cggcgggcgc gtgttcgata taaatatcaa acggtttttt ggttgaaacg
     8341 gcggcggcgc ggacacggct tccgtccggc agcgtgcaac cctcggtcaa atcgcattgc
     8401 gccgccacgg cttgcggctg ctgcgcctgc caccattgca acaaaacgag cttgaaggcg
     8461 gcaaaggcag tcagcagcag tgcggcaagc agtaatttgc ggtttttatt catgattctt
     8521 ccctattgtt tcatccctta ccaccgcgtg cgcccccaat ccgagccata gttttccata
     8581 acgtgcaacc gtctgtccgt ttgctgcaag tacgagcggc acggatacgc cctgccgcag
     8641 ggcttctgcc gcctcttgtg cctgaacatc atcgcacacg cgccacacgg ccacatccgc
     8701 gcccttttcc tgcgcctgcc gccattgttc gcgggtctgc accatcatcc acacgctgcc
     8761 ggcgtgttcg gacctgtcct gccattgtgc cgcctccatc aaaacgttcg caccgcttcc
     8821 ccctgccgaa cccaaaggca ggacgcggta cgcgcccata ctgttttcgc cgtacaaacc
     8881 tgttttcagg ctgccgtaca aacggcgcgg gacggacagc gaacgcaaaa gcgcaccgtt
     8941 ggcaggcagc atgggggcaa cggtaaaatc acccgccttc tgccaagacc attcctgccc
     9001 ttcgcgcgat tgcggtccgc cctcccattg atcggggttt acccataaga atttcaggca
     9061 gacgcgggcg tgttcgtagg aatggatttt ggtcagccaa ggcgtggcgg cgaggatgcg
     9121 gatgccgagt tcttctccaa actcgcgttg cagggcttgg aagtcggttt cgcccgcctc
     9181 gaccttgccg ccggcaaatt cccaatatcc ggcatagggc ttgccttcgg ggcgcgagcc
     9241 gagcaggtaa ttgccgtctg aatcgagcag gatgccggca acgacgcgga taagggggcg
     9301 ggtatcttgg atcatggcgg gtatgcggtt ggtcggtcgg aatgcaaatg agggaagggc
     9361 aatgccgtca acgggtttgc cgaatttcag acggcattat ttttcggcaa cggcaaacgc
     9421 caacacggta tcgccttcgt ccgccatact caggctgacg cggctgatgc cttgttcttc
     9481 cagccattcg gacagggcgg ggccgtagaa aaattcgggc ttgcccaatg cgtcgtgccc
     9541 gatgccgatg ttgcagaagg aaaccgcgcc tcgtatgccc gtgccgacgg ctttggcgaa
     9601 ggcttctttg gcggcaaagc gtttggcaag gtagttgacg ggtttgcccg cctgcggaaa
     9661 ttcaagcagc tcttcgggag tgagtatgcg ccctgcaaac gcctgtccga attttttgtt
     9721 taagcggatg atgcgcttga gggaaacgat gtctgtaccg atgccgtaaa tcatatttgc
     9781 gctccttttt cgtgaaatgc cgtctgaaac ggtttgcctg cccttacggc agcagccttg
     9841 ccctgaacat cgcctccttc atctggcgca cggcttcggg aagtccgagg aagagggctt
     9901 gggcaatcag cgaatgcccg atgttcagtt cgcggatggc gaggatttgg gcgacggggg
     9961 taacgttgtg tatggtcagt ccgtgtccgg cgttgacgac caagcccaaa tcgccggcga
    10021 aatgcgcgcc gttttggatg cgctcgaact gcctgatttg ttcggcgtgg ctttgtgcgt
    10081 cggcatatgc gccggtgtgc agctcgacaa cgggcgcgcc gacatcacgg gcggcttgga
    10141 tttgcctgtc gtcggcatcg ataaacaagg acacgcgtat gcccgcgtcg gtcaggattt
    10201 tggcgaattc ggcgattttt tcctgttgcg ccaatacgtc caaaccgcct tcggtcgtga
    10261 tttcctgccg tttttcaggc acgatgcaca cgtcttccgg catcacttta agcgcgtttt
    10321 cgagcatttc ttccgtcaac gccatttcaa ggttcaggcg cgtgcggatg gcgtttttga
    10381 cggcaaatac atccgcgtct ttgatgtggc ggcggtcttc gcgcaggtgc atggtaatca
    10441 ggtctgcacc gtgcgtttcg gcaaccagtg ccgcctccac ggggctggga taagtcgtgc
    10501 cgcgcgcgtt gcggacggtg gcgatgtggt cgatgttgac acccagaagc ataatgtttc
    10561 cttttctctt cgttttcctg tttgacggca tggtgcgggc gcagctacgc ggtattgcgg
    10621 tcgaactgcc ttatctgttc caacacctgc cgcgatttca agccttcggg aagcagcgtg
    10681 ccgattagca gccgtgtgat tttcaatgcc tgttgcaggc tttcggcggt gcggaaactg
    10741 ccttcgcgca aatcgatcag gctctgccct tcgacaattg cgccggcttc gtgcgaaagc
    10801 gcgcctgtgt cccggcatac gggcatgacc gcctcttcgg gcatcagccg gtaggttttg
    10861 tccgccaaaa tatcgccgcc cgtgccgtcg gcgtgcaaat cgggggcaac gcccaaggca
    10921 ttgagcagct tccattcgaa gcggcgcaaa tcggcaatat ggttggcttc acggcacact
    10981 gcctccatca cttttgccaa cgcgtcgtac agttcggaca tcgggtcttc gcgcgcggtc
    11041 agtttcaaca ccagctcgtt cacatacaga ccgctgaaca acgccctgcc ctgcggctgc
    11101 cgccatccgc ccatccattc ggcgcggtgc agggttttga gttcctgact gccgtaccac
    11161 gacacgctgg cagggacgaa cggaaccaat acgccgcgca gctcgctctg ccgtttgcgc
    11221 gcgctgcgcg ccagcaaagc cacacgcccg taacggcggc tgaatgcttc aacccgcagg
    11281 ctgctttcgc gccaaggcga agatgccagc ataaaaacgg gttcgtggtt gactcggtat
    11341 tcggacataa tcgttgtgga aatgccgtct gaacgctgcc gcgactacaa aacggcagtc
    11401 ggatacgaac cgatggcttt gacaaacgaa gcgcgttcgc ccaagcgttc caatgccgtc
    11461 tgaatctgcg cgtcccggcg gtgtccttcg atgtcgatga agaacaggta ttcccacaaa
    11521 acggatttgc tcggacggct ctcaaacttg gtcatggaaa tacccgattc ggtcagcggt
    11581 tgcagcagcg aggcaaccgc gccggcgcgg ttgggcgcgg aaacggccag cgaggttttg
    11641 tcgctgccgc ttgcaccggt ttcgtgatgt cccatgacca gaaagcgcgt ggtgttgttc
    11701 ggttcgtctt cgatgcactc ggcaaccata tcgagtccgt aaatttccgc cgccgtgcgt
    11761 ccggcgatgg cggcaaccgt accgtcgtcc gattcggcaa ccagccttgc ggcttcggca
    11821 ttgctggaca cggcaatccg ttcggcattg ggcagacgcc tgcccaacca gtcgttgcac
    11881 tgcgccaacg cctgcgcgtg cgaaaatact ttggcgatac cctcggtgct gccgttgttt
    11941 ttgcgcaaca ggttgtggtg gatgcgcaaa acgacttcgc cgcacgcctg caacgcggta
    12001 acggcaagca aatccaatgt gcgcccgacc gagccttcgg tcgagttttc cacgggcgcg
    12061 accagataat ccgcctgacg ggtttcgacc tgcttgaagc agtcgtctat ggtcggacac
    12121 gccattgtat gcgcggcgtg tccgaaatgc ttgattgccg cctgttgggt aaacgtgccc
    12181 tgcggcccca gataggcgat ggtcagcgga cgttcgacgg cgaggcactc gctcatcact
    12241 tcccgaaaca ggcgtgctac cgattcgtcg ggcagcgggc ctttgttcaa atcctgaatg
    12301 cggcgcaaca cggcaacctc gcgttcgggg cggtacaccg cgcccgtgcc tttcagctcg
    12361 ccgatggcgt gggcatgttg cgcgcgttcg ttgagcaggc gcagaatttc ggcatcgatg
    12421 gtgtcgatgg cgttgcggtg cgggatgagg agttcgtcga tagtttggga cataatttcg
    12481 cctgaacggt aatttgccgt gcattctaac atcaaatgcc gtctgaagcg gtttcggacg
    12541 gcatcggttt acaagtattc gctttttttc gttttccttc cacgccttgt gcgagcagag
    12601 ccacagaagc gcgatgccgc aagaggtagg gccgccatag ttgccgccat cacagttgcc
    12661 gaaccgtcgt gcaggacggt tgccgccatg cccacgcccg cgccgattaa ggaccggaat
    12721 acacccgaca cggcgttcgc gctgccgccc tcttctttga aataagacat aaagcatgcc
    12781 tgcgtgtccg caccgaccag gccctgcgta ccgacggaaa acatcacgca cgcaaccggc
    12841 agccaaaacg ggggcaaccc gaaaaacagc acggcggcga gttgggacgg gttggcggca
    12901 aattggacga caatcccccg cagcaggatg ctttgcggat gcgcgccggt tttaagccgc
    12961 cacgcggtaa cgcggctgaa aaacatcatc gtgatgatgt tgagtgcaaa cacccatgcg
    13021 taccggtgcg gcgtaacgtg gtagagctgc cggtacacga aggaagattc ggtcagaaag
    13081 gcgaacatcg aaccgaagct gaatgcctga aaaaacagat aacccatcgc ggcacgggtt
    13141 ttcaatacgc gcttgaaccg ccccgccacc agcccgaaca catccctgcc gattttgccg
    13201 ccgacggcgg gattgggcag gaaatactgt accaaaccgg gcagcaccgg cgaatacgcc
    13261 gccaagaaaa cgaaaatcgc ccgccatccg cccaatccct gcaacaatgc gccgaccatg
    13321 ggtccggcca gcggcacaac catcagaatg atgccgataa gggcaaacat ctgcgcggct
    13381 ttgcgtccgg aataataatc gcgcaccatc gcaccgacga tgactacagc catgcctgcg
    13441 ccgaacgcct gtaccgcacg caggttaagg agctgttcgg tactcgaagc aaatacgatg
    13501 gcggcaacgg caaggcaata tacaatcaaa ccggtcaggg cgacgggttt gcgccctttg
    13561 atgtcggaca ccgcgccgcc ggccacttgc ccgaacgccg tgccgaacat aaacaaactc
    13621 agactctatt cgatacggtg gatatccgcg ttcagcggct gcgccatttc gggaatcgcg
    13681 ggcaggtagg catcggtgga aaacggcatc agcgcgacca gtaccgccat caaaaccgcc
    13741 atcagttttc cgctcatcgg aggaaggggc ggagacatgg tttttccgtt caaatcaaat
    13801 aaagaaaccg tcgcaaacga cggtcggtac gggcgcaagt ctaacatatc ggacggcggg
    13861 gcaaaaccga aggatatgcc gcccgaaacc gcttcagacg gcattgccgt tttgcgcgtc
    13921 cctgcgcgac agccggccgg cggcgaaagt ggcggcgatg acgacaaaca cgcccaaggc
    13981 ggacacgggc gataaatgtt cgcccaaaat caaaaccgcc aacagcacgc cgacgacggg
    14041 ttcgagcgaa atcaacagtc ccgacgcgtt ggcaggaaca cggctcatcc ccttgttcca
    14101 cagccaatag gcgtaccagc cgcaccccaa acccaaatac aacagcgaca ataccatccc
    14161 gacgctccag tccacggtat aactttgcgc caaagcaagc gaaaacggca ggcacatcaa
    14221 cgatgcggcg gcaatggaaa cagatgtgaa tgccggtgcg ccgatgcggg caatcagcct
    14281 ttgcgtcgga cgcatagcgg cacaaaagcc cgcgcccgcc aacaacacca gcaggcagcc
    14341 gaaccagccg acttcgccgc cctcttccgc accgcccgcc atcagcagcg cgacaccggc
    14401 aaatgccgcc gcgccgcata tccagtggta ggcacgcgct ttgtcgttga agaaaaagtg
    14461 tccgacaaac accatcagca gcggctcgag tccgacaatg accgatgcgc tggcggcgga
    14521 agtgtatttc aacccgacaa actgaagcag cagggtcagc acatagttga cgaacgacac
    14581 aatcagcaac ggcttccatt cctcacgcgg aatcttgccg acatgacggc ggcaggcggg
    14641 cagtgcaggc agcgcggcaa tcagcaggcg cacgccgacc atcaatgcgg gatcgatgcc
    14701 gccatagaca tatttggcgg caataaacga gctgctccag ataatcaggg caaggatttg
    14761 gtaaaacata gtgagtggta aagtcagatg gtcatacaaa agtatagcgg attaacaaaa
    14821 atcaggacaa gaaggaatgc ccgaaccgtc attcccgcca cttttcgtca ttcccgcgaa
    14881 agcgggaatc tggaatctcg gactttcaga taatctttga atattgccgt tgttccaagg
    14941 tccggactcc cgcccgcgcg caagtttccg aagccatcct tttggccgaa ggtcaaaaat
    15001 cagccgttac cgagtattac ctgaataacg gcgaatggcc gaaagacaac acttctgccg
    15061 gcgtggcatc cgcttcagaa atcaaaggca aatatgttca aagcgttacg gtcgcaaacg
    15121 gcgtcgttac cgcccaaatg aaatcagacg gcgtaaacaa agaaatcaaa gacaaaaaac
    15181 tctccctgtg ggccaagcgt gaaaacggtt cggtaaaatg gttctgcgga cagccggtta
    15241 agcgcaccgc caacaacaac gacaccgttg ccgccgacaa agacaaa
//
LOCUS       DAXFWM010000061        14690 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000061 DAXFWM010000000
VERSION     DAXFWM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.061"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            415..782
                     /locus_tag="ACFI8B_001699"
                     /pseudo
     CDS             415..782
                     /locus_tag="ACFI8B_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            779..1090
                     /locus_tag="ACFI8B_001700"
     CDS             779..1090
                     /locus_tag="ACFI8B_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3834866.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            1200..1412
                     /locus_tag="ACFI8B_001701"
     CDS             1200..1412
                     /locus_tag="ACFI8B_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834867.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGAKPETSPTARHGDFLRP
                     LQGGIGFKRHGLEGKTGGAQNCLNAV"
     gene            complement(1862..2417)
                     /locus_tag="ACFI8B_001702"
                     /pseudo
     CDS             complement(1862..2417)
                     /locus_tag="ACFI8B_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2414..3221)
                     /locus_tag="ACFI8B_001703"
                     /pseudo
     CDS             complement(2414..3221)
                     /locus_tag="ACFI8B_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3214..3384)
                     /locus_tag="ACFI8B_001704"
                     /pseudo
     CDS             complement(3214..3384)
                     /locus_tag="ACFI8B_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(3351..4142)
                     /locus_tag="ACFI8B_001705"
     CDS             complement(3351..4142)
                     /locus_tag="ACFI8B_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC3834868.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(4371..7001)
                     /gene="leuS"
                     /locus_tag="ACFI8B_001706"
     CDS             complement(4371..7001)
                     /gene="leuS"
                     /locus_tag="ACFI8B_001706"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC3834869.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVSDNAFDANRWQEWYGNKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            7268..7784
                     /locus_tag="ACFI8B_001707"
                     /pseudo
     CDS             7268..7784
                     /locus_tag="ACFI8B_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            7791..8234
                     /locus_tag="ACFI8B_001708"
     CDS             7791..8234
                     /locus_tag="ACFI8B_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC3834870.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            8403..10460
                     /gene="ppk1"
                     /locus_tag="ACFI8B_001709"
     CDS             8403..10460
                     /gene="ppk1"
                     /locus_tag="ACFI8B_001709"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC3834871.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(10609..11712)
                     /gene="dnaN"
                     /locus_tag="ACFI8B_001710"
     CDS             complement(10609..11712)
                     /gene="dnaN"
                     /locus_tag="ACFI8B_001710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC3834872.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(11792..13348)
                     /gene="dnaA"
                     /locus_tag="ACFI8B_001711"
     CDS             complement(11792..13348)
                     /gene="dnaA"
                     /locus_tag="ACFI8B_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC3834873.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            13640..13774
                     /gene="rpmH"
                     /locus_tag="ACFI8B_001712"
     CDS             13640..13774
                     /gene="rpmH"
                     /locus_tag="ACFI8B_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC3834874.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            13777..14142
                     /gene="rnpA"
                     /locus_tag="ACFI8B_001713"
     CDS             13777..14142
                     /gene="rnpA"
                     /locus_tag="ACFI8B_001713"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC3834875.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            14207..14428
                     /gene="yidD"
                     /locus_tag="ACFI8B_001714"
     CDS             14207..14428
                     /gene="yidD"
                     /locus_tag="ACFI8B_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC3834876.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPAP"
BASE COUNT         3498 a         3944 c         3744 g         3504 t
ORIGIN      
        1 cgccgtaccg gtttaaattt aattcactat aaaaataaga aaaagataaa aaattggtaa
       61 caaatgcggt aacaaatggt aacgaatcgg taacaacttt tggggttttc cggtttttta
      121 ccgtcttggc agtgggagcg tagcggaatg aaaagccaaa acgcacggaa ccgcgcctat
      181 tttgagcagg aatggcggtt aaaccggttg gttatattgc ggggcatagg aagacagcga
      241 aacgcgctaa aaacgcaatt tgagccatta aaagcgattg attaaaaaat aatcaaatta
      301 gccgccggtg tttcaggcgg cataaacgga gaaattgcag ggtataaaaa ggcggcttgc
      361 cgtgttgcct gtctttggta taatccccag tatcactaag taaacggttg cacaatgcgg
      421 atattcaaaa accaatggat agtgaaattt gccaagaagc acaaaatcag tgattcagag
      481 ctgatagagg cggtagagcg ggcagataac gggctgatag atgcagattt gggcggcgcg
      541 tgattaagca gcgcatagca aggcaagggc aaggcagaag cggcggttat cgcagtctga
      601 tatttttcag gcatggagaa cgggcgtttt tcatgaccgc ctttgccaag aacgaccgcg
      661 aaaacatcac tgataaagaa cttacagaat taaagaaaac cgccgccatc atcttggcaa
      721 tgacagaaac cgacatagag caagccaagt caaacggcat tttcacggag atacagccat
      781 gaaatataaa agcgggattt tcgcagcaat acacgaaacc atgcagggat tgcacgacat
      841 cggcgcaatc gacaaaaaga ctatgcgcgg ctttgacaag tcctgcctga ccgaaatcaa
      901 gccgttgagc ggcggggaca tcaaggcaat cagggagaag gaggcactat cgcaagccgc
      961 tttcgccatc tatctcaacg tggggaaaaa tcacgtttcg gcttgggagc ggggcgttaa
     1021 aaagccgagc ggcgcggcgt tgaagctgct gaccatcgtc aaaaacaagg gcatcgaagc
     1081 cattgcgtag ccgacttggc aaacggcaaa atcagcaagt tcacaataga cgcgctgctg
     1141 aatatgcctg ccaagacagg caagaccgcc gaactgaata tcagggcgta gccgcataaa
     1201 tgcccgaccg catcaaacca agccgaaacg gcggcggtgc agacggcata gcccgacagc
     1261 aaggcacggc gcagacgggc gcgaaacccg aaacatcccc gaccgcgagg cacggggatt
     1321 ttttgcgccc gttgcagggg gggattggat ttaagcggca cgggcttgaa ggcaaaacgg
     1381 gcggggcgca gaactgttta aatgccgtct gaatctcaaa ccatttcaga cggcattttg
     1441 aaacaacggc tcaaattctc gatccccttc ccttaacgcc gacgtttttt attaacgcgc
     1501 cccttatttc tgacactttg ctcataaacc ggcataacgg tcggcaacaa ccgttttaga
     1561 ttttctatac gggcattgtt tacagcatca gcataagaca ccgtactctg gaataccgcc
     1621 taaaccctgc ggatagtttt ggatgtggta tcgacattgt cggcagactt gtctaactcg
     1681 accgttttca aataatcttt ggcagatgct gcattcagtg tcgcggaatc cgcgtatttt
     1741 gaacaaaagg tcgggctact ttatcacaat agcagaaaga tggaaaacgg cggggattgg
     1801 tgaaaggtcg tctgaaatga tattcagacg accttgaacg gttgtgtttt aaatatggta
     1861 tttattcatt tgtttcacca tcaggcagtc caaaccacct taagggaatc gtgttaaatt
     1921 ggtaatggca gttaacactg ataatataat ctaaaacatc tttaatattc cggttttgta
     1981 aaccaatcat tcaaaacata gacatatcca acccgccaac ctaaagagtg aactaacttt
     2041 gtatattgtt ttctcttaaa atcgcaagtt tgtaatttct catctactga accgggagtt
     2101 tgttggaatt tgatctcaat aataaataag gtatcacgga tgatgataaa taagccatta
     2161 tcgggcagta atcttttaga cagatggtct ttccaattaa tattgtattc cagtctttcc
     2221 aaaaatcggt aaaactcata ttttttgaaa caataagcct tcaattctcc attaaaccaa
     2281 acttcataac ccgcattatc atctgttttt cttaagtcgt acccgggaat ttcttcaaac
     2341 aactttcgta agtctgtacg ttcttcgaat ctgagtcctg tttgtgtcct tgcaccgccg
     2401 attccgccaa taatcatttt tctaaccttt tctttgctaa ttcaaaaaat cttcttccag
     2461 ttcgcaaccg caaaatctcc gaccatgttt taaggcggca acgcctgttg tgccgctgcc
     2521 cataaaaggg tcaaaaatta aatctccgat atttgaagcc gatagtatgc agcgttcaag
     2581 taaggagagt ggtttttgtg tcggatgttt gccgaatgtt ttttcggttt tatttggtgg
     2641 aggaaatgtc caaacacatt tcatctgttt gccattattt tgtgctttca tcatttcata
     2701 atgaaacgta tgtttggctt ttttattttt ctttgcccat aagattgttt ctgtcgaatg
     2761 ggtaaagaaa cggcaggaca aattaggggg aggattgggt ttttcccaag taatattgtt
     2821 caaaatatgg tagccgacgg tttgcatcag gtagccgatt aaatagatat tatgaaatgt
     2881 gccgcaaacc caaattgtgc cgtttggttt taataaggcg taacacagtc ggagccattc
     2941 ttcgtaaaat tccaaatctg ccgccattcc tttggattta tcccagttgc ccttattgac
     3001 ggaaaccatt tgcccgtttt gacaactgaa accgtcattg gaaagaaagt aaggcgggtc
     3061 tgcgaaaatc atatcaaaac agccgtttgg atgtttttcc aatatcttcc gcatgacgtt
     3121 aagggaattt tcgttgtaga ggacaaggtc gtctgaaacg attttttcaa acgactttga
     3181 taaatggcag cttcttcagc cggtttgtca aaatcaatca taaattgcca ataattctcc
     3241 gactttttta cgactgctgc cctttgcggc aatattgcgt tgtacttcaa ccgtttttag
     3301 aatggcagaa gaataaatat ttctcgtaaa ttcagtatca tgatttgaaa tcaaaacgcc
     3361 ttgggagttg ggtgcaattt gtcgattttt ttgtaaagtc cgcgaccaat gaattcgatc
     3421 atattttgat cacgcaaaat ttgcaactgt tggcggattt tgtctttgat atggttgttt
     3481 tggggaaatt ggatggatag tttgttttca aattcataca tttgcgacaa tgtgaattct
     3541 tcggtgagtt ggtcgataca tttcataaca gccaaaagcc agtctttgcg ctccgcattt
     3601 tggttgcgta aaaacaggtt ggattgccat tttttcagaa cggtttcggg ttcgataatg
     3661 cgggaatcgt ctattaagaa tatttttccg ctttcaggca aaggggcgag attgatggaa
     3721 cacataatgt ggtgcggtcg gtttttaatg cctttatttc tgggaataat catatccggc
     3781 gtgatgaaat gtttgggtac aagcaccaat tgccgtatgg agtaatccgc ttttttatat
     3841 gcaagaaaga aaaagttggg gttggtatct gaccggatgc gctccaacat agtgcgatat
     3901 gcaccgtcag gcacgctgtt gcctatggtt ttttgatttt tactctttaa ttcatattgc
     3961 tcgtggcaat ttgggcaaaa gaggtctgca acaggtttgt tattggcaaa tctctgcatc
     4021 ggcttgcttc cgcaacaggg gcaatagccg tttttttcca gccaagcctc gctcattaca
     4081 cggattttgt gggttgcttt attttgttgc tttcccaatt gggtatcgaa aaataaattc
     4141 atattttgga ttttgagatt aatttttagt agattaacaa aaatcaggac aaggagacga
     4201 agccgcagac agcagggcaa ggcggggcaa cgccgtaccg gtttaaagtt aatccgctat
     4261 atttgaggtc tgtcttgcag acaacccaat ctgccttaaa aaggccgtct gaaaccctgt
     4321 ttccaagttt cagacggcct ttatccgtat ggctaaacct taaaagcggt ttagacgacg
     4381 atgttcacca gtctgcccgg tacgacgatg attttcttcg caggcttgcc ttccatgaat
     4441 ttcactgcgc cttcgttggc gagtgcggcg gcttcgaggt cggctttgga ggcgtcggcg
     4501 gcgacggtga ttttgccgcg cagtttgccg ttgacttgaa ccatcacttc gatttcggat
     4561 ttgaccaggg cggcttcgtc gactgtcggc cagcctgctt cccacagttt cgcgccgttc
     4621 aattcgctcc acagggtttc gcagatgtgc ggcacgatgg gccacaacag gcgtacggcg
     4681 gcttccaata cttcttgggc gacggcgcgg ccttgttcgc tgccggtgtc ggttttgtcg
     4741 tattggttga gcagttccat cacggcggcg atggcggtgt tgaactgctg gcggcggccg
     4801 tagtcgtcgc tgactttggc ggtggtggaa tgcagtttgt gacgcaggtc tttgagttct
     4861 ttagacaaac cgtcttggtt gcctgcaaat gctttgaccg cgccgccttg cttcaggtat
     4921 tcgtaaacgg tacgccacag acggcgcagg aagcggtgtg caccttcgac gccgctgtcg
     4981 ctccattcga gggactgttc gggcggtgcg gcgaacatca tgaacaggcg ggcggtgtcc
     5041 gcgccgtagg cgttaatcag ttcttgcgga tcgacgccgt tgtttttgga tttggacatt
     5101 ttttccgtgc cgctgatgac gacgggcagt ccgtcggctt tgaggacggc ggaaacgggg
     5161 cggcctttgt catcgaaagt cagctcgaca tcggcggggt tgatccagtc tttgccgcct
     5221 ttgtcgtttt cgcggtagta ggtttcgcag acgaccatac cttgcgtgag caggcgttca
     5281 aacggttcgt caacattgac caaaccttcg tcgcgcatca gtttggtgaa gaagcgcgcg
     5341 tacaggaggt gcaaaatcgc gtgttcgatg ccgccgatgt attggtcgac cgcgccccag
     5401 tatttcgcgg attctgccga taccatgccg tctgaaaact tgggcgacat atagcggaag
     5461 aagtaccagc tcgactccat gaaggtgtcc atggtgtcgg tttcgcgttt cgccgcgccg
     5521 ccgcagcacg ggcaggaagt ttcgtaaaac tcgggcattt ttgccagcgg cgaacccata
     5581 ccgtcgggta cgacgttttc aggcaggacg acgggcagtt ggtcggcagg gacgggaacg
     5641 tctccgcatt tttcgcaatg gacgatggga atcgggcagc cccagtagcg ttggcgcgaa
     5701 atgccccagt cgcgcaggcg gtattgggtt ttcggttcgc ccgcaccttg gctttgcagc
     5761 ttggcggcaa cggcatcaaa tgccgtctga aaatccaagc cgtccaagtc gccgctgttg
     5821 accaatacgc cgttttcttt gttgccgtac cattcttgcc atcggtttgc gtcgaatgcg
     5881 ttgtcgctga cggcaatgac ttgttttttc ggcagattgt atttggcggc aaactcgaaa
     5941 tcgcgttcgt cgtgcgccgg aacagccatc accgcgccgt cgccgtagcc ccacaagaca
     6001 tagttggcaa tccacacttc cagcttgtcg ccgttgagcg ggttgacgac gtagcggccg
     6061 gtcggcacgc cttttttctc catcgtcgcc atatcggctt cggcaaccga accggctttg
     6121 cattcggcga taaatgcctg caattcgggt ttgtcggcgg ctgcggctgt tgccagcgga
     6181 tgttcggcgg caacagcgac ataagtcgca cccatcagcg tgtcggggcg ggtggtataa
     6241 acttgcagga atttcgcgta atcgccttcc aaaccttgct tgctgtcgtc tgaaacggcg
     6301 aagcgcacgg tcatgccgcg agatttgccg atccagttgc gctgcatggt tttgacttgt
     6361 tccggccagt gttccagctt gtccaaatcg ttgagcagct cttcggcgta atccgtgatt
     6421 ttgaagtaat acatcgggat ttcgcgtttt tcgatcaacg cgcccgaacg ccagccgcgt
     6481 ccgtcgatga cttgctcgtt ggcaaggacg gtttggtcga ccgggtccca gttcaccgtg
     6541 ccgtttttgc gatagacgat gcctttttca aacagcttgg taaacagcca ttgttcccag
     6601 cggtagtatt cgggtttgca ggtggcgact tcgcgttccc agtcaaccgc aaaacccagg
     6661 cttttgagct gggttttcat gtattcgatg ttgtcgtagg tccaagcggc gggggcgacg
     6721 ttgtttttca tcgccgcatt ttccgccggc atgccgaacg cgtcccaacc cataggctgc
     6781 ataacgttga agccgtttaa aagtttgaag cggctcaata cgtcgccgat ggtgtagttg
     6841 cgtacatgcc ccatatgcag cttgccgcta gggtaaggga acatcgaaag gcaataatat
     6901 ttgggtttgg aagcgtcttc ggagacgttg gaaatacggg cgtcgtccca ttttttctgc
     6961 gccgcaggct cgatggcggc gggctggtaa tgttcttgca tggtcattct gtttttcgct
     7021 tgaaaacgtt aggaatttaa aggaggaatt ataacaggtt gccgatgtcg gcgaatcggc
     7081 aaattgccgg caggatgcgt aaattcgcac gcgcatcatt ccgtatgccg tacaaataca
     7141 ccgcgtttac tgatacgcac gttttttatg ctaatattac gaaccaaaat caaacgttta
     7201 aaactctcct gatgcggctc ttccgaacaa aaggcagacg ggcatcgggt aaaaaaggat
     7261 tctgcatatg aaaatcaaac aaatcgtcaa accgagcttg gcagtattgg cggcaggcat
     7321 tctgtctgcc tgcgcaacca taaagcaacg tcaaagccga cggcacgacc gacaatccgg
     7381 ttttcccgga accctattcc gtaacgctcg acaacaagcg cggcacattc ccgacttatg
     7441 acgaactgga tcagatgcgc cccggtctga ccaaagacga catctacaaa atcccgggcc
     7501 gcccgcatta cgacgaaggt atgtacggcg tgcgcgaatg ggattacctg ttccacttcc
     7561 acaccccggg cgtgggtatc gaccctgaaa acacttccgg cgtggaaggc gttactacct
     7621 gccaatacaa agtgattttc gataaagaca aatttgcccg cagcttctac tggaaccccg
     7681 tcttcccgaa agatgccgtc tgcccgccgc ccgcacccaa agccgagccg caagtcatca
     7741 tccgcgaaat cgtgccggca aaaccgaaac gtatccgcca ataagccgct atgccgcccc
     7801 gcctgctttc agggatattg tgctgcctgc tgacgactgc ccccgtattc gcacaggggc
     7861 agccggatgc ggtcagtgcc tatattcaaa agaaaaaagt catcgtcgac acatccaagg
     7921 cggaactctg ttttgccgac gaccgtcagt gccaccccgt cctcatcggc actgccacac
     7981 ccaaggggac gttcggactg acgctgaaca gtaccgacaa gcccggttac ggcggcgaag
     8041 tcatcggttt caagcaggag ggcgattttc ttttcgccct gcaccgcgtt tggaatcaga
     8101 taccgtccga aaggcgaaac gaacgcatcg cctccccgtc cgtgtccgac aggattatga
     8161 ccaacggctg catcaacgtc agcgatgcgg tgtacgaaaa actgcgtcat tattttgtgt
     8221 tggaagtgat ttgaaacaga cggataccgc acgtaccggt atccgttttc acattgcccc
     8281 gatgcctgaa acagaccgtc cgtcacgtca tgccgcctga aaccggcgca gatgccgcca
     8341 agccttcaga cggcattgtc cgcccgctcc gaccgaacaa caaccatctt tgggagaacc
     8401 ttatgaccga acaaaaccgc atcctctgcc gcgaactgag cctgctggca ttcaaccgcc
     8461 gcgtgttggc acaggcggaa gacaaaaacg tccccctttt ggaacgcctg cgcttcctgt
     8521 gcatcgtgtc gtccaatctt gacgagtttt ttgaagtccg catggcgtgg ctcaagcgtg
     8581 aaaacaagct gcacccccgg cgcaggccgg acaacggcaa aatgccgtct gaaaccatcg
     8641 ccgacgttac cgaagcggcg cgctccctga tacggcacca gtacgacctg ttcaacaacg
     8701 tccttcagcc cgagttggcg cgggaaagca tccattttta ccgccgccgc aattggacgg
     8761 gcacgcagaa aaaatggatc gaagactatt tcgaccgcga actgctgccg atcctgaccc
     8821 ccatcggact cgacccttcc caccctttcc cgcgcccgct gaacaaatcg ctcaacttcg
     8881 ccgtagaact cgacggcacg gacgcgttcg gcaggccttc gggtatggcg attgtccaag
     8941 caccgcgcat cctgccgcgc gttgttcccc tgccgtccga actgtgtggc ggcggacacg
     9001 gcttcgtctt cctgtcgtcc atcctccacg cccacgtcgg caaactcttc ccgggcatga
     9061 acgtcaaagg ctgccaccag ttccgcctca cgcgcgacag cgacttgacc gttgacgaag
     9121 aagacgtaca aaacctccgc gccgccattc aaaacgaact gcacgaccgc gaatacggcg
     9181 acggcgtgcg gctcgaagtc gccgacacct gtcccgccta catccgcgac tttctgctcg
     9241 cgcaattcag gctgaccgac gccgaactct atcaggtcaa aggcccggtc aacctcgtcc
     9301 gcctcaacgc cgtccccgac ctagtcaacc gccccgattt gaaatttccg ccgcacacgc
     9361 cgggccgtct gaaagccttg ggcaaaaaca gcccgatctt cgatttggtg cgccaatcgc
     9421 ccatcctgct gcaccacccc taccaatctt tcgatcccgt ggtcgatatg atacgcgaag
     9481 ccgccgccga tcccgccgtt cttgccgtca aaatgacgat ttaccgcacc ggcacgcgct
     9541 ccgaactcgt ccccgccctg atgaaggcgg cactcgccgg caaacaagta accgtcgtcg
     9601 tcgaactgat ggcgcgtttt gacgaagcca acaacgtcaa ctgggcgaag cagctcgaag
     9661 aggcgggagc gcacgtcgtg tacggcgtgt tcggctacaa agtccacgcc aaaatggcac
     9721 tggtcatccg ccgcgaagac ggcgtgctca agcgttacgc ccacctcggc acgggcaact
     9781 accaccaagg cacatcgcgc atctacaccg acttcggcct cattaccgcc gacgaacaaa
     9841 tcaccgccga cgtgaacaca ttatttatgg aaatcacagg cttgggcaaa cccgggcggc
     9901 tgaacaaact ctaccaaagt ccgtttaccc tgcacaaaat ggttatcggc cgcatcgctc
     9961 gcgaaaccga acacgcaaaa gccggcaaac cggcgcggat taccgccaag atgaattcgc
    10021 tcatcgaacc gactgtcata gaagccctgt atcgggcaag cgcggcaggc gtgcaaatcg
    10081 atttgattgt gcgcggtatg tgtaccttgc gcccgggtgt aaaaggcttg tccgaaaaca
    10141 tccgcgtccg ctccatcgtc ggcaggcagc tcgaacacgc gcgcgtatat tgtttccaca
    10201 acaacggcgc ggacgacacc tttatttcca gcgcggattg gatggggcgc aatttcttcc
    10261 gccgcatcga aaccgccacc ccgattaccg cgcccgaact caaaaaacgc gtcatccgcg
    10321 aaggtttgga aatggcactg gcagacaata cccacgcgtg gctgatgcag cccgacggcg
    10381 gctatatccg cgccgcgccc gccgaaggcg aaagcgaagc cgacctgcaa aacgatttat
    10441 gggatttgct ccgaggctga cccgcacaag cacttccccc atacgctccc aaccccaatc
    10501 cgccgcattc cagacggcat tgcctttccg acagccgaac aaaaccccga atccgtctgc
    10561 acggttcggg gcatatctcc aatacgggca tcgtgttccg gaaaaccgtc aaatccgcat
    10621 cggcatcaca atatatttga aattcggatt gttcggcacg gtaaacagcg tcgagcggtt
    10681 ggcgtcgccg aaggcaagct gcatatcgtc ggaatggatg ttgcgcaaca cgtccatcaa
    10741 atagccgata ttgaaaccga cttcgagttc gccgccctga taggcgattt cgatttcttc
    10801 gcgcgcttct tcctgctcgt tgttgctgca cacgacgctc aacaggccgg gttgcaggaa
    10861 caggcgcgcg ccacggaatt tttcgttggc aagaatggac acacgttcca gcgcgcccaa
    10921 aagttcggca cgggacaaca cgaaaatctt gtcgttgtcc aaaggaatca cgcggttgaa
    10981 atcggggaat ttgccgtcga tgaccttgct gacgatggtc gtgccgttgc attggaaacg
    11041 cacctgcttg tccagcagct cgatttgaat cggatcgtcg gggttgttca acagtttgaa
    11101 cagttccagc accgttttgc gcggcaaaat cacttcggcg cgcggcaaat ccgcatcaat
    11161 cgcgcaggct gcataggcaa ggcggtgtcc gtcggtcgcc acaaggcgca actggctgcc
    11221 ctcaacctgc atcagcagac cgttgagata atagcggatg tcctgcaccg ccatgctgta
    11281 ctgcacttgc gacagcatgg ttttgaaacg ctcctgcccc agcgagaaag tcgcgctgat
    11341 gtcctcgccg acattcatca tcggaaaatc ggcggcaggc agggtttgca gggcaaaacg
    11401 cgatttgccc gccttcagcg tcagacggtt gtcgtcccaa tccagcgaca ccagcgcacc
    11461 ggcaggcagc gcgcgcaaaa tgtcctgaaa tttcttggca ttggtagtga tgcggaagtc
    11521 gcccgcacct ccctcgggac ccgcggtgtc gatttggatt tccaaatcgg ttgccaagag
    11581 tttggtctga ccgcccctgc cctcaatcag cacattggac aggatgggca gggtgtgtcg
    11641 gcgttcgacg atgccggtaa cggcttgcag cggcttgagc aggctgtcgc gctcggcttg
    11701 taaaatcaac attttcgctc cttaaatcgg ttggatttcc tacccttcgg acggcatggt
    11761 caatgtcatg ccgtctgaaa ggcgtgtccg gtcagttttg aatcagaatc agcagtttct
    11821 cgtaatcctg cgccaactcg gggtcttcct cgcgcagttt cgccaccgcc ctgatgccgt
    11881 gcatgacggt cgtatggtcg cgtccgccga acgaatcgcc gatagacggc aggctcaaag
    11941 tggtcaattc tttggtcagg ctcatggcaa cctgacgcgg acgggcaatg ttgcgcgtgc
    12001 gttttttgcc gagtacgtcg ctgattttga tgcggtaata tttcgccacc gcatcgatga
    12061 tgatgtcggc ggtgatgact ttgtgcttct cggcaataat gtcctgcaaa gcggtacgcg
    12121 ccaaatcgat gtcgatgacg ggacggttca taaagcggct gctcgctccg acacggttga
    12181 acgcgccttc cagttcgcgc acgttggaac ggatcagatt ggcaatgaac agcgcggctt
    12241 cgtcttcgat actgatgccc gccgcttccg cctttttctg caaaatggcg atacgcattt
    12301 ccaattcggg cggctcgagt tccaaagtca gcccccacga aaagcgggat ttgaggcggt
    12361 cgtccatacc ttcgattttg gcgggtaaaa catcgcaagt gaggatgagc tgttttttct
    12421 cattgtgaaa atggttgtac agatagaaaa attcttccat cgtacggtct tttcctttga
    12481 tgaactgaat atcgtcgata atcagcaggt cgtattgctt gtattgctgc ttgaacacgt
    12541 cgtaggtatt gttgcgaacc gccttcataa agctgcggat gtagtcgtcc gaatgcatat
    12601 agcgcacttt ggcatcggga cggtttttca acagctcgtt gccgaccgcc tgcacaaggt
    12661 gggttttgcc caaacccgtg ctgccgtaca ggaagaacgg gttgtaactc tgccccgggt
    12721 tttccgcaat cgcctgcgcc gcagccgccg caaggcggtt gcccttacct tctaccaacg
    12781 tatcaaacgt gtaatccgga gacaggttgg tttgttcgta acgcgcttct tccgcatcac
    12841 gctgcgcatc cgtccgcgct ttggcaactg ccgccgattc cggccgggaa gcaggcccgg
    12901 cagcctgacg cggctcgtgc ggcaggtttt tcatacgttc cgccaaaata tccgccgccg
    12961 ttttcgacgc agcgggtttg acaggctttt cagacggcag ctcgtccaac agaacctcct
    13021 gcacgggcat ctcctccgac cccgcgtgca aggacggctc ggcaggttcg acagcacctt
    13081 caaccgccgc catctcataa cgcacgcctt ctcccggttt gaatacgaag gcgggacggc
    13141 cggcagccaa ctcttccctc accgcttcta tttttccggc aaactggctc ttgagcatat
    13201 tgcaggcaaa ctggttcttg ccgtacacca cccatacgcc accctcctca ccaaccgtaa
    13261 ggggcgcaat ccattgcgca aactgcccgt gaggcaacat atcgtgaaga cggcggaggc
    13321 acagcggcca aaactctgct aatgtcatgg ataggctcga atcggtaaaa atgaaatcga
    13381 aaacaaagaa aatataatat tttcaaaaag aaaacaaatc tgttgaatac gcgtcggttc
    13441 aaaacgcagc gaaccgatta taccgactcg ccgatatttt atccacaacc cgtgcaaaaa
    13501 tttatccaca gaaaggcggc agaaatccgc aggcaatcgg gcaatcctcc tgcaaagttt
    13561 ctatattgat tgacaaaagc ggcaaattgg agtgtaattc acggtttaat tatctaccca
    13621 ttctatttta ggaaccatca tgaaacgcac ttatcaacct tccgttacca aacgcaaacg
    13681 cacccacggc ttcctcgtgc gctccaaaac acgcggcgga cgcgcagtat tggccgcacg
    13741 ccgtgccaaa ggccgcaaac gcctggcagt gtaattttgg actaccgctt cggaaggcag
    13801 taccgcttgt taaaaacgga tgatttttca tccgtttttg cattcagaaa ccgccgcagc
    13861 cgcgacctgc tgcaagtttc gcgctcaaac ggcaacgggc tggaccatcc ccgcatcggt
    13921 ctggtggtcg gcaaaaaaac cgccaaacgc gccaacgagc ggaactatat gaaacgcgtc
    13981 atccgcgact ggttcagatt gaacaaaaac cggctgccgc ctcaggattt cgtcgtgcgc
    14041 gtccgccgga aattcgacag ggctaccgca aaacaggcaa gggcggaact ggcacaactc
    14101 atgttcggca accccgcaac cggatgcggg aaacaggtat gatcagaacg gtactctgca
    14161 cgcaaggttc agacggcaac gggtttccca tacaaggaac atcccgatga acttcctatt
    14221 gtccaaactg ctgctggggc tgataaggtt ttaccaatac tgcatcagcc cgctgattcc
    14281 gccgcgctgc cgttatacgc cgacctgttc gcaatacgcg gtcgaagcgg tcaaaaaata
    14341 cggcgcattc aaaggcctcc ggctcgccat caagcgcatt gcacgctgcc accctttcgg
    14401 cggacacgga cacgaccccg ctccctgacc cgacgcaata ttcaaattgc acgctttcct
    14461 tttatttccc atcggtttct atataatgcc gtctgaacct tcgggctggc ggcacggccg
    14521 tcgggtatga agcccgccct tattccccgt ctatcggaac acgcaacctg cggcatttcc
    14581 gaccattcag gaaactctat atggatttta aaagactcac ggcgtttttc gccatcgcgc
    14641 tggtgattat gatcggctgg gaaaaaatgt tccccacccc gaaacccgtc
//
LOCUS       DAXFWM010000062        14367 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000062 DAXFWM010000000
VERSION     DAXFWM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14367
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.062"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..798)
                     /locus_tag="ACFI8B_001715"
     CDS             complement(<1..798)
                     /locus_tag="ACFI8B_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC3834877.1"
                     /translation="MKTGIQTELAQALLPHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPASQPASQP
                     ASQLYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQPVGEIKRRSDG
                     TPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKPQDSFG"
     gene            complement(1015..1935)
                     /gene="truB"
                     /locus_tag="ACFI8B_001716"
     CDS             complement(1015..1935)
                     /gene="truB"
                     /locus_tag="ACFI8B_001716"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC3834878.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLIDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDNLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASAA"
     gene            complement(1993..2517)
                     /locus_tag="ACFI8B_001717"
     CDS             complement(1993..2517)
                     /locus_tag="ACFI8B_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834879.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            complement(2528..2899)
                     /gene="rbfA"
                     /locus_tag="ACFI8B_001718"
     CDS             complement(2528..2899)
                     /gene="rbfA"
                     /locus_tag="ACFI8B_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC3834880.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            3082..4326
                     /gene="clpX"
                     /locus_tag="ACFI8B_001719"
     CDS             3082..4326
                     /gene="clpX"
                     /locus_tag="ACFI8B_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC3834881.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(4433..5626)
                     /locus_tag="ACFI8B_001720"
     CDS             complement(4433..5626)
                     /locus_tag="ACFI8B_001720"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC3834882.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(5725..5934)
                     /locus_tag="ACFI8B_001721"
     CDS             complement(5725..5934)
                     /locus_tag="ACFI8B_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834883.1"
                     /translation="MGWISSKRKKAPHVCVVPIAVSGCKRFALHDEMPSEDKNIGEIL
                     NQNAAAPQALYRNFFVFFSYPVENI"
     gene            6031..6531
                     /locus_tag="ACFI8B_001722"
     CDS             6031..6531
                     /locus_tag="ACFI8B_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834884.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            6616..7170
                     /locus_tag="ACFI8B_001723"
     CDS             6616..7170
                     /locus_tag="ACFI8B_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834885.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            7184..7384
                     /locus_tag="ACFI8B_001724"
     CDS             7184..7384
                     /locus_tag="ACFI8B_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834886.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            7515..7621
                     /locus_tag="ACFI8B_001725"
                     /pseudo
     CDS             7515..7621
                     /locus_tag="ACFI8B_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7735..9123)
                     /locus_tag="ACFI8B_001726"
     CDS             complement(7735..9123)
                     /locus_tag="ACFI8B_001726"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3834887.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            9451..10398
                     /locus_tag="ACFI8B_001727"
     CDS             9451..10398
                     /locus_tag="ACFI8B_001727"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3834888.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(10450..10782)
                     /gene="trxA"
                     /locus_tag="ACFI8B_001728"
     CDS             complement(10450..10782)
                     /gene="trxA"
                     /locus_tag="ACFI8B_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC3834889.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            11080..11553
                     /gene="dnrN"
                     /locus_tag="ACFI8B_001729"
     CDS             11080..11553
                     /gene="dnrN"
                     /locus_tag="ACFI8B_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC3834890.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            complement(11624..12445)
                     /gene="nadE"
                     /locus_tag="ACFI8B_001730"
     CDS             complement(11624..12445)
                     /gene="nadE"
                     /locus_tag="ACFI8B_001730"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC3834891.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHRTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            12642..13997
                     /gene="xseA"
                     /locus_tag="ACFI8B_001731"
     CDS             12642..13997
                     /gene="xseA"
                     /locus_tag="ACFI8B_001731"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC3834892.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT         3366 a         3763 c         3819 g         3419 t
ORIGIN      
        1 accaaaacta tcttgtggtt tctttttaac aaaataataa ggcgggtcaa tataaatcag
       61 cttcactttg cctttaaact gtttggcaag cgaatgcagg gcaatcagat tattgccttt
      121 gataataaga ttttcggctg gcgtaccgtc tgaacggcgt ttgatttcgc caacgggttg
      181 tttgccgtct gcggtgtggc gggagaattt tgagaatgct tttgcgtcaa aaagccggtc
      241 aatttcatca aaagcaagcg tttgattaaa aaagatttct tgtctttttc tggttaattt
      301 ggtgtataat tggctggctg gctggctggc tggctggctg gctggctggc tggctggctg
      361 gctggctggc tggctggctg gctggctatt attgcgttta aaataaattt cttcgccttc
      421 ttcggtgctt tgtccgccat tcaatacgca atctttaaac ggaaaatcca acacaatatc
      481 cgaattgtct ttcaaaaagc ggttgccgtc cgtcaaacca atgcggttgg cgtattttgt
      541 gtaggaatta ttgatgctgt gtttgtccaa gaaaaaacga aaatcctgca atttaaacac
      601 caaaacacca ttcacttcca caaagaaatg gcgttttaac tcatcattat tcaataacaa
      661 accgataatg gacgggtcgg ttttttccac caaatccaac aggatgtttt tggccaaaat
      721 ggttttttct tcgttcgccc atattttttc gtgtggtagc agggcttggg ctaattcggt
      781 ttgaatgcct gtcttcatcg ttataaatcc tcatcagtta ttttaatgtg ccattcttgt
      841 gtaacggcaa atggctttac aaataaatta taataaaatc aattctttgc tgtctttgct
      901 gtttccgggg gcgttcgcaa cgttgcgata agcggggcgg gtgtccgaat gcatcgggat
      961 tttgcggaaa tatcaacata ttattcaaac acagcccgta tttttaaccg ccgctcaggc
     1021 ggcggatgcc gccgtgttca tcaggcgcaa ggctttcata cggcatattt ccttttgata
     1081 ttccgccaaa ccgacaaagc ggccgttttc cgtgtaaacg cgtaccggcg tatcggaagg
     1141 caggtcttcc tcgaaacgcg gccgttgccc gcattggagc atatggacgg cataatcgtt
     1201 taaaacggtt tggggaaagt gtgaaaccaa tacgtcgcag ggcagcagca ggttgtcgcg
     1261 ttctgtttcg tccaagtttg ccaaggcttc gagcgtgtgg ctttgggcga tggtaaagcc
     1321 ggcggtttcg gtgcggcgta gggcggtcag gtgggcgaat gtgccgatgt gtttggcgat
     1381 gccttcgctg agggtgcgga tgtaggtgcc tttgctgcaa cgcacgctta tcacggcttt
     1441 gggcgcgtca aactcggcaa tatcgataga gtaaacggta atgtcgcgcg gtttgcgttc
     1501 gataacaatg cctttgcgcg cgtattcgta caacggtttg ccttcgtgct tgagggcgga
     1561 aaacatcggc ggcacttggc ggatgttgcc tgtcagtgct tggcaggccg tctgaaattc
     1621 ggctaaggaa atatcggcgc gggcggcggc gatgatttct ccttcggcat cgcccgtgct
     1681 gctggcttcg ccgagtttca gcgtggcggt gtaggcttta tcggcatcaa tcagatattg
     1741 ggcgaacttg gccgcttcgc cgaagcagac gggcaaaagt ccggttgcca aaggatcgag
     1801 cacgccggta tgtccggctt tttcggcatg gaacaaacgc cgcgcttttt gcagggcggt
     1861 gttgctggaa aggccttcgg gtttgtcgag aagaagaacg ccgttgaccg ggcgtttggc
     1921 gggtttattg gtcatattaa ttagggaata ttaatgttca gaccgtctga aagggttcgg
     1981 acggcctgcg tgtcagctta attcttcatt caataattca cgaacagagt gtttttcatc
     2041 aattagatca atgctgattg catcaaagcc atttcgtttt aatgagtcaa aactaatgat
     2101 gcggccatca attaagggga cttgtccttc agaattgacg aaatcttcat attcatcttc
     2161 gtagtctata ttgtaataaa gatgaatcgg aactcggtaa gtatattcat ccaaatcagt
     2221 taacgttctg ttttgctttt gattttggct atatttgtcg cagaacatag cgttatagcc
     2281 atgttccgcc ccatcaaaca atacgatttc atcgccgctt acagcttcta ccgccacaat
     2341 ttttaccggc gccttatcag tatcggtaat caatccatca aaaggctctc catagtaacg
     2401 gatgtggaaa gtatctctac cttggcagga taatgaaaag caacgatgcc cgccttcaat
     2461 ttgcaggtta tcactatgtg cataggcggc taaatgttcg ggaatcagat tgttcattct
     2521 ggcagtatca gtcttcaacc ggtttttccg ccgccacttg atcgataagg gcggacaggc
     2581 tcataccgcg ttccaaagat tcgtcgtatt tgaaatgcag ttcggggatt ttgaacagct
     2641 tgatgcgttt ggacaattcg ctgcggaggt gtccgcgcgc gtgttccagc acttcttccg
     2701 taatttcgcg cgtatcttgg ttcaaaacgg tgtagaacac ggtggcgtgg ctgtaatcgc
     2761 gggtaacttc gacttcgttg atagtgatga agccggcgcg cgggtctttc agtccggtac
     2821 ggacgagttc ggcaagctcg cgcataattt gttctttgac acggtcttgg cgggcgtagc
     2881 cgcgttgggg ttttctcatg aatatcctca gatttggtgt cagacggcat ttctgccgcc
     2941 ggggcggttg cgaaagggcg tattatagcc gaatcggcgg gattttaagg taatacgttg
     3001 ataagcacgg atatagttat tatacttggc aacccgaacg gcgcatgccg tttctgcaac
     3061 gcatacattg taaagaaagc catgtccaac gaaaaccgta cctgttcctt ttgcggaaaa
     3121 tccaaatcac acgtcaaaca tttgattgag ggcgaaaacg cctttatctg cgacgaatgc
     3181 gtctcaaact gcctcgaaat attgtacgaa ggcgacaacg gcggcacgcc tccggaaaat
     3241 gccggagggg agccggaaga atccggcaaa ctgcccacgc ccgccgaaat cgttgccaac
     3301 ctcgacgatt atgtcatcgg gcaggggcag gcgaaaaagg cgctggcggt tgcggtttac
     3361 aaccattaca aacgcctgcg ccacccgaaa gccgacggcg gtgtcgaatt gtcgaaatcc
     3421 aacatcctgc ttatcggccc gaccggatcg ggtaaaacgc tgttggcgca atctttggca
     3481 cgcaaactgg acgtgccgtt cgtgatggcg gatgcgacca cgctgaccga agccggctat
     3541 gtcggcgaag atgtcgaaca aatcattacc aaactgttgg gcaaatgcga tttcgatgtc
     3601 gaaaaagccc agcacggcat tgtctatatt gacgaaattg acaaaatttc gcgtaaaagc
     3661 gacaacccgt ccatcacgcg cgacgtgtcc ggcgaaggcg tgcagcaagc cttgctgaaa
     3721 ctgattgaag gtacggtggc aagcgttccg ccccaaggcg gacgcaagca tccgaatcag
     3781 gaatttatca acgttgatac cgccaacatc ctgtttatct gcggcggcgc gtttgcaggc
     3841 ttggaaaaag tgattcgcca gcgcaccgag aaaggtggta tcggtttcgg cgcgtccgtt
     3901 cacagcaagg acgaaaatgc cggcattacc aagctgttcg gcatcgtcga accggaagat
     3961 ttaatcaaat tcggcctgat tccggaatta atcggacgtt tgcccgtgat tgcaacttta
     4021 gaagaactgg atgaggacgc gctgattaat attttaaccg agccgaaaaa cgctttggtc
     4081 aagcagtatc aagccttgtt cggcatagaa aacgtcgggt tggaatttga agaaggcgca
     4141 ttgcgttcca tcgcgcggca ggcaatggaa cgcaaaaccg gcgcgcgcgg cctgcgttcc
     4201 atcgtcgaac gctgcctgct cgatacgatg taccgcctgc ccgatttgca aggcttgaaa
     4261 aaagtggtgg tcggcaaggc agtcatcgaa gagggcaggg aaccggaatt ggtgttcgag
     4321 tcctgatggc aaacaaaacc gcttcggaac gttggatcac gtttccgaag cggttttttg
     4381 tttctgatgc cgtctgaggc cttcccgtct tcagacggca tctgctttct gcctaggggc
     4441 ggtttgcggc ggcaaattcc gtcagcacgt gttccaaacg ccgcaaacct tccgcaatat
     4501 cctcatcgtt caacagcagc gaaggcgcga aacgcaatac gtccgcaccc gcaactaaga
     4561 tcatcacgcc gtgtttcaag gaggtagcgg cgatttcgga tgagcgtccg cgataaggcg
     4621 tgtccaacgc gcagccgagc agcagcccca tcccgcgaac ctgtgagaac aagcctgttt
     4681 tcctgcccaa atccagcaat gccgtctgaa gtttctgccc ctgactgcgg acgttgtgca
     4741 aggtttccgg cgcgttgatg atgtcgaagg cgcggctgcc gaccgcacac gccatcgggt
     4801 tgccgccgaa agtcgagccg tgcgtgcccg gttggaaggc ggcggcaatc ttttctgtcg
     4861 ccagcatcgc gccgatcgga aagccgcagc ccaaggcttt tgccgaactc aaaatatcgg
     4921 gaacaacgcc gtaatgttca taggcaaaca gcctgcccgt atgccccatc ccggtttgaa
     4981 cttcgtccaa aatcaacaac gcattgtgcc ggtcgcacag acggcgcgcc gcctgcaaat
     5041 attccgcagt ggcgggcagg atgccgcttt cgccctgtat cggctcgatg atgaccgcgc
     5101 aggtctgttc gccgacggca gcttccagcg cggcaatatc gttgaacgga acgtgcgtaa
     5161 tgccttgcgg cagcggtgca taatccttac tgtatttcgg ctgtccgccg acggacacgg
     5221 taaacagcgt gcgtccgtgg aaactgttga tacaggcgac gatttcgctt ttgcctccgc
     5281 cgaaacggtc gcgcgcatat ttccttgcca gctttaacgc cgcctcattc gcttccgcgc
     5341 ccgagttgca gaaaaaaacc ttgtcggcaa aactgtgttt caccaatttt tgcgccaatt
     5401 cctgcgccgg ccgcgtcgta tagatattgg aaatgtgcca cagcttgtgc atctgcgcgt
     5461 ttaaagcatc gacaagggca gggtggcagt gccccagagc gttaacggcg atgccgcctg
     5521 aaaaatcaat atattcacgc ccttccgtat cccaaacgcg gctgcctgaa gcgcgttcgg
     5581 gaatcatcgg ggcaaaggcg aaattggggg tcagatagtt ttgcataatt gctcctgatt
     5641 gcggttttgt tcgggattat gcaccgtttt tcaaggcgga taaagggaaa ttgccgacaa
     5701 tatcgggcag tggtaaaaat aaacctaaat attttcaacc ggatagctga aaaaaacaaa
     5761 aaaatttcga tataatgctt gaggcgcggc agcgttttga tttagaatct ccccaatatt
     5821 tttatcttcg gacggcattt catcatgcaa ggcaaaacgt ttacaaccgc ttactgctat
     5881 tggtaccaca cagacgtgcg gtgctttttt gcgcttgctt gaaatccatc ccatacgcaa
     5941 aacaggccgc tcgaaaacgg ggcggctttt ttgttacctt cacacaggtc gcccgattcg
     6001 gcaacacacg acagaatcag gaaaatgacc atggatgcaa tgccccaaac cgccgtacaa
     6061 aacgaaaacc tgcacacttg cagcattatt gtcgatgatg cgcaggattt ttcccccatg
     6121 cttctcgatg cagggcggga cgacacgctc atcagcgaat cgatgattcc gcaaatccgc
     6181 accgttgccg ccctgatagc cgccgaacgg cacgacttca gccgcagcag ccccgccgag
     6241 tttaccgatg ccgccgattt ttttgccgca cggattctcg tattgggcgt gcgacgcttc
     6301 catctggacg tatcgctgct tcagattttg aaaaccgcaa acaagcgcgc ctgccgcttt
     6361 gccgaaaaac accgcctgtt tttcacaccc gcacaagccg aattgagcct gaacaaacac
     6421 aaaaaccgcc gcctgctgac catagaaacc gaacacgaag tcgaaaacaa aggcaaccct
     6481 gtcgccaaca gcctcgcatt tgtccgcaaa ctgcacaccc tacccctgta aacatcacga
     6541 tttgccggca gaatgcttgc caaaattccc gacggcgata caatacaacc gattttccac
     6601 ccgaacagga cacacatgaa aaaaatcatc gcctccgcgc ttatcgcaac attcgcactc
     6661 accgcctgcc aagacgacac gcaggcgcgg ctcgaacggc agcagaaaca gattgaagcc
     6721 ctgcaacagc agctcgcaca gcaggcagac gatacggttt accaactgac tcccgaagca
     6781 gtcaaagaca ccattcctgc ccaggcgcag gcaaacggca acaacggtca gcccgttacc
     6841 ggcaaagacg ggcagcagta tatttacgac caatcgacag gaagctggct gctgcaaagc
     6901 ctgattggcg cggcggcagg cgcgtttatc ggcaacgcgc tggcaaacaa attcacacgg
     6961 gcgggcaacc aagacagccc cgtcgcccgt cgcgcgcgtg ctgcctacca tcagtccgca
     7021 cgccccaatg cgcgcaccag cagggatttg aacacgcgca gcctccgtgc aaaacaacag
     7081 gcggcgcagg cgcagcgtta ccgcccgaca acgcgcccgc ccgtcaatta ccgccgtccc
     7141 gctatgcgcg gtttcggcag aaggcggtaa acccggcgcg ccaatgccgt ctgaagggct
     7201 ttcagacggc atttttgtat ttgttagggg cattgttatg ttgccgtttg attttcagac
     7261 ggcattttgt ttccaagcgt ttgatgtcgg gatggcaatt ctgatctacg tgcgttggca
     7321 gggcggcatt gtcataccgc cgttgggttt tcagacggca tcatatcgaa ccgtcaagcc
     7381 gtagggcgag attgggtagg caggaatgcc catcctgcat ctgaaaccct tctgcgtcat
     7441 tcccacgcag gaaggaatct agatatttga atttacggaa attttcaatc gttgccaaag
     7501 cggaaaaggg tttttatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
     7561 agtacagata gtacggcaag gcgaggcaac gcggtaccgg tttaaattta atccactata
     7621 ttgcagatta atcctttggg tatcaaagcc gggcggtgct gaaacattac aaaaatgccg
     7681 tctgaaactc gaaatcggtt tcagacggca ttggtttacg cggcaggttg gggcttagcg
     7741 gtctttgtaa cggcgttcgc ttttgcctgc aaaagagtcg ccggctttgc gtttgccttt
     7801 gaagccttcg ccggtttttt tgaaactgtc tttcttaaag ccttctttct tgaaaccttc
     7861 gccgcgcgtt ttgccgccga agccttcttt gcccggttta tgatcgccgc gccggccgcc
     7921 ggatttccta tcgccccagc cgcctttgcc tttcggcttg ccgcctgcgg atttgcgttt
     7981 gcgggccggc tccatgcctt cgatggtcag ttcgggcagt ttgcggttaa tgtatttttc
     8041 gattttgtgg actttgacgt attcgttcac ttcggcaaac gtaatcgcaa tacccgtgcg
     8101 gcctgcgcgg ccggtgcgcc cgatgcggtg gacgtagtct tccgcctgtt tcggcaggtc
     8161 gtagtttatg acgtgggtaa tggtcggtac gtcaataccg cgtgcggcaa cgtcggtggc
     8221 aaccaaaatt ttgcagcggc ctttacgcaa atccgtcagc gtgcggttgc gccagccctg
     8281 cggcatatcg ccgtgcaggc agttggcggc gaaacctttt tcgtacaatt catccgcgat
     8341 gacttcggtc atcgctttgg tggacgtgaa aatcacacat tggtcgatgt tggcatcgcg
     8401 caggatgtgg tcgagcaggc ggtttttgtg gcgcatatcg tcgcagtaca acaactgctc
     8461 ttcgattttg ccttggccgt ccacgcgttc gacttcgata atttcagagt ctttggtcag
     8521 tttgcgcgcc agtttgccga ctgcgccgtc ccaagtggcg gagaacaata aagtctgacg
     8581 gtcggccggg gtggcttcca cgatggtttc gatgtcgtcg ataaagccca tatccaacat
     8641 acggtcggct tcgtccaaaa tcagcacttc caaacgttca aaatcaactt tgccgctttg
     8701 catcaggtcc gtcagacggc ccggagtggc gacaatcaaa tcgaccggtt tgctcagggc
     8761 gcgggtttgg tagccgaacg atgtgccgcc gacgatgctg acggtgcgga accagcgcat
     8821 atttttggca tacgccagcg cgtttttctc gacttgcgcc gccagttcgc gggtcggtgc
     8881 caacaccagc gcgcgcgggc ctttgcccgg tttttcgctg cgtttggtca gtcgctgcaa
     8941 agtcggtaac agaaaggctg cggtttttcc ggagccggtt tgcgccgaag ccatgatgtc
     9001 gcagccttcc aaagcaaacg gaatagcttg cgcctgaatc ggcgtcgggc tttcgtaacc
     9061 ctcgctgctg acggcggaca aaatgttttt atcaaggttc aaatcggcaa atttaataga
     9121 catggctatc cttggaagac aacaacgccc gcgtccgaaa ggttcagacg gcctgaagca
     9181 gggaagggta acgatacggg gaatgtgaag ttacagggtt gtcgcaagcg gcttgcttgt
     9241 ggtttacatc gacggcaacc tgcacagata cgctttgcaa gatcaaagca acaaaagaaa
     9301 cgcgcccgta agggcggttt aggttggaag gcgatggctt cgtatgacga tataagcaga
     9361 gaatggtata gggtttgccc gaaaaggtca agcaatcggg cgttaattac agtaaaattt
     9421 ccgttttcca ctttattccg cctgaaatca atgaccgaca tcacaccgtt tgccaaccgc
     9481 ttgggcaaaa acatcaaaca ccttatgaaa tgggcgaaac gcaacggcat cgaagcctgg
     9541 cgcatttacg accgcgacat cccccaattc ccttttgccg ccgatgttta cggcgatcga
     9601 atccaccttc aggaatacga taccggctgg ctgatgcggc ccggagaata cgaagcgtgg
     9661 cttgccgaag tattggaagc cgtcgctttc gttaccggtt ttgaacccga acaaatccgt
     9721 ctcaaacgcc gcgaacgcca aaaaggtttg cagcagtacg agaaaaccgg caaggcgggc
     9781 gacgatttcg tcattgccga aaacggccgc aagttttggg tcaacctcga caaatacctc
     9841 gacaccggct tcttcctcga tcaccgcaac acgcgcaaaa aagtcggcga aacggcggcg
     9901 ggcaaacgtt ttttgaacct gttttcctac accggcagct tcaccgtcta tgccgccacc
     9961 ggcggcgcgg catccagcga aaccgtcgat ttgtccaaca cttatctcga ttgggcgagg
    10021 cgcaattttg aattgaacgg catcgatatg gaacggcaca aaatcgtccg cgccgacgtg
    10081 ttccaatacc ttcagacggc atatggggaa ggcaggcagt tcgacctcat tgtgatggac
    10141 ccgcccagtt tttccaacag caagaaaatg cccggcatcc tcgacatcca gcgcgaccat
    10201 caaaaactca tagacggcgc ggtgaacctg ctcgcttcag acggcatttt gtatttttcc
    10261 aacaacctgc gcagcttcgt tttggacgat tcggtatcgg aacaatacgc cgtgaaagac
    10321 atttccaaac aatccgttcc cgaagatttc cgcaacaaaa aaatccaccg gtgttgggaa
    10381 atccgccaca aatcttaagg gtgcgaaaaa gccgccggaa tcttccggcg gcttttcgat
    10441 tgcactttat caggcgatgg aggcttcgac aaaggcggtc agctgacctt ttgccaatgc
    10501 gccgactttg gtggcgacga cttcgccgtt tttgaacacc atcagggtcg gaatgccgcg
    10561 cacgccgaaa cgggacgggg tggcttcgtt gtcgtcgatg ttgattttga ctactttcag
    10621 acggccttca aattcggcgg caatgtcgtc caaaatcggg gcaatcattt tgcaggggcc
    10681 gcaccacgga gcccaaaagt ccagcaggac ggggaggtcg gatttcaaaa catcttgctc
    10741 gaaggcggca tcgccggtgt gtacgattaa ttcgctgctc attatttttc cttttcggtt
    10801 gggattcgca cgatagcgtg caggataggg cttgggcata aaaatttcaa gtgggcgggc
    10861 gggataatag tttttctgta ccgccccaat cgcctttgca aattctaaaa aacggttcgg
    10921 acagtatttt atttttgaaa attactttta ttatcaaagt ataaggaatg tttttgaggc
    10981 tgtacagaaa cgcgcgggcg gtgtatgctt cgtttaaata gattcttaaa gtatgaatgt
    11041 tcaaaaccaa acccgaaacc ctataaaagg aacactgcaa tgactgattt ttccgtttgg
    11101 gaagctgctc cctttggcgc aaccgttgat catatcctgc aacgctacca caatgtccac
    11161 cgcgcacaat ttgaagaatt ggtgccgctg gcgcagaaag tggcccaagt ccatgccgat
    11221 acctttccgg cggaaattgc cgggctgctt gccgatatgc gggacgagct gctgatgcat
    11281 atgatgaagg aagagcggat gctgtttccg atgattaatc agggtgtcgg acgcggtgcg
    11341 gcgatgccca tcagcgtgat gatgcacgaa catgaagaac acgaccgcgc catcgcacgg
    11401 ctcaaagagc tgaccggtaa tttccacgca cccgaagggg cttgcggaag ttggacgcgc
    11461 ctttacgcgc tggcaaaaga aatggcggac gatttgaacg accacattca tttggaaaac
    11521 gatattctgt ttgcccgcgt actcgattct taagggatag gagtcggata tttcaatgcc
    11581 gtctgaaata cggtttttcc gtttcagacg gcattttcat gtttcagccc agcaattcgg
    11641 gcggaatgcg gcacacagga atcgggttga ttttgtgctg catcgcgcgg tgaagccgcg
    11701 tatagatttc cagaacttcg cgccgccgtc cttcaaaatc ttcgggtttg cgcgtgccgt
    11761 acacgcccat tgcccactcc agttcgggat agcttgcgcc catctgttcc tcgtcggtgc
    11821 gttccgtatc ccacaggccg tcggtcggcg gggctttttg aatcgcctcg tccacgccca
    11881 atgcttcggc aagccggtaa acctgcgttt tggtcaggtc ggcaatcggg ctgatgtcca
    11941 cgccgccgtc gccgtatttg gtaaaaaaac ccacgccgaa atcttcaacc ttattacctg
    12001 tccccgtaac cagcagtccg tgtatctgcc cgtagtagta cagggtcagc atacgcaggc
    12061 ggcttctggc gttggcgagc gaaagcggct gattggcaaa tgccgtccga tgaacgccga
    12121 cggtttgctc aaaggtttgg aaggtgtcgg tcagattggc cgtttgcgcg cttacattgg
    12181 catattgccc ttgcagattg cggatgtgcc gccttgcccg ctcgagctgg ccggggtgtt
    12241 ggcgtatcgg catatccaga agcagcgtgg tgcggccggt gcgggcggcg agcgtggaga
    12301 cgacggcgga atcgatgccg ccggaaacgc ctacgacgaa cccttttgca tttgcctgag
    12361 cggcgtattc gtccaaccat cggacaatat gtgtgataac tgcctgcgta tccatttttc
    12421 ctcctgtcga aaatatccgc atcatagcct gccggcggtg caatttgaat agattgaggg
    12481 cgggttgatg aatatttttc ccattccccg cttaaaaaag tcatatagga aaaggctggc
    12541 ggcaagatgg cgcgtgttat aatattgttg acaatttaga cggcacgatg ccgccttggg
    12601 cttcaaaaca tattcatatt ttttattatt tgattgattc gatgtctgat ttttttcatt
    12661 ctgacgtgtt gtccgtttcc gagttgaacg catttgccaa aagtattttg gaaaaccacc
    12721 ttgccggcct gtggattgcg ggcgaagtgt ccaatctgac ccgcgccgcc agcgggcatt
    12781 attatttctc actcaaagac agccgcgcgc aggtgcgttg cgcgatgttc aagggtgcgg
    12841 cggcgcgttt ggcgcaacct ttgaaagaag gcgaccatat cgaagtggcg ggcaaaatca
    12901 gtatttacga ggcgcggggc gaatttcaga ttaccgtaaa cgaagtacgg ctcaaaggtt
    12961 tggggcagct ttacgaagcc tacgagcggt tgaaggcgca gttgcaggcg gaaggcgcgt
    13021 ttgcggcgga acgcaaaaaa cctttgcccg tccgtccgca atgtatcggc atcgttacca
    13081 gtctggcggc ggcggctttg cgcgatgtcg taaccacctt gaaacgccgc gcgcccgaaa
    13141 tccccgtcat cgtttatccg gccgccgttc agggcgcggg cagcgggttt cagattgccc
    13201 aagcgattaa aaccgcatcg caacgcgccg aatgcgatgt gctgattgtc tgtcgcggcg
    13261 gcggcagcat cgaagacttg cgggcgttta acgaagaacc ggtcgtgcgc gccatcgaag
    13321 cctgcacgat tcccgtcgtc agcggcgtgg gacacgaaac cgatttcacg ctcgccgatt
    13381 tcgttgccga cgtgcgcgcg cccacgccga ccggcgcggc ggaactggtc agccccaacc
    13441 gccaagaatc actgcaccgc ctcgtccaag cccaaggccg tctgaaaacc gttttggagc
    13501 aacgctattt cgatgccagc caaaaactcg actggctcgc gcggcaaatc cgccacccgc
    13561 gccaaaaact tgacgagcag cgcgcttcaa tcggcaagct ggcgcaaacg ctgtcttact
    13621 cgatgacgca aaacctccgc gcccacaccg cccgttttga acgccaaacc caagccctgc
    13681 aacattgccg tcctgatgtt tccgtttacc ggcaagatat cgtccgcctt cagacggcat
    13741 tgcccgccgc cttcagccgg ctgctcgccc gtcgccgtca aagcctgacc gcccaagccg
    13801 cattgctcga agccgtttcg ccgcagcaca ttctggaacg cggcttctcc gttgtcaaaa
    13861 atacacgcgg acaagtcatc cgtaacgctg atgttttgaa gcaggggcag aagctgcaca
    13921 ttaccttctc cgacggcgaa accgacgtgc gcgtaagcaa agaacaaggg cagcaggatt
    13981 tgttcgattg catttaaacc caatcggaaa aaccgctccc cctccgccgt cattcccgcg
    14041 aaagcgggaa tccagtccct tgaccttaag ataatcttta attcgtcatt cctgcaaaag
    14101 cgggaatccg gaagcggaaa gccgccgggg tttatcagaa atgactgaaa ctcaaaaaac
    14161 ggattcccgc ctgcgcggga atgacgattg atacgtttcg ataatttatt gaagatggct
    14221 gaaattcaaa gttacagaaa tttataaaca aaaaaacagc aacctgccgc cgtcattccc
    14281 gcgaaaacgg gaatccagtc cctaaaaagc gggaatccgg aagcggaaag ccgcaggaat
    14341 ttgtcggaaa aaacagaaac ctctccg
//
LOCUS       DAXFWM010000063        13889 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000063 DAXFWM010000000
VERSION     DAXFWM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.063"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(253..1103)
                     /locus_tag="ACFI8B_001732"
                     /pseudo
     CDS             complement(253..1103)
                     /locus_tag="ACFI8B_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            1453..2967
                     /gene="katA"
                     /locus_tag="ACFI8B_001733"
     CDS             1453..2967
                     /gene="katA"
                     /locus_tag="ACFI8B_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC3834893.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            3483..4049
                     /locus_tag="ACFI8B_001734"
                     /pseudo
     CDS             3483..4049
                     /locus_tag="ACFI8B_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            4271..5434
                     /locus_tag="ACFI8B_001735"
     CDS             4271..5434
                     /locus_tag="ACFI8B_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFC3834894.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(6015..8051)
                     /locus_tag="ACFI8B_001736"
     CDS             complement(6015..8051)
                     /locus_tag="ACFI8B_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC3834895.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELEQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMTIYSEDNEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8139..9011)
                     /locus_tag="ACFI8B_001737"
     CDS             complement(8139..9011)
                     /locus_tag="ACFI8B_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC3834896.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            9277..11667
                     /gene="gyrB"
                     /locus_tag="ACFI8B_001738"
     CDS             9277..11667
                     /gene="gyrB"
                     /locus_tag="ACFI8B_001738"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC3834897.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            11766..13151
                     /locus_tag="ACFI8B_001739"
     CDS             11766..13151
                     /locus_tag="ACFI8B_001739"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC3834898.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(13222..13320)
                     /locus_tag="ACFI8B_001740"
     CDS             complement(13222..13320)
                     /locus_tag="ACFI8B_001740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC3834899.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            13617..13766
                     /locus_tag="ACFI8B_001741"
     CDS             13617..13766
                     /locus_tag="ACFI8B_001741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834900.1"
                     /translation="MNQIIFAETLKNLPYLPDRHQDFVVVTEKNILKVRIPNQSLKYR
                     NHIRF"
BASE COUNT         3268 a         3767 c         3776 g         3078 t
ORIGIN      
        1 accaatcccg ccaccaacct caccggctca aaccggcaca gcaacttata tcccgtcatt
       61 ctcgcgcagg cgggaatcca gccccgccgg tacagaaact tatcgaaaaa aagtttcttc
      121 agattttacg ttctagattc ccactttcgc gggaatgacg ggatgcaggt ttccgcgcgg
      181 acggattcgg cattcccgct ttcgcagaaa tgtcggcttt tggtttgttg tctttataag
      241 attattcggt aattattctg cggttttgcg tttgtgtgct gccggaatct caagggattc
      301 tctgtatttg gcaacggtgc ggcgggaaac ctctataccg cggaaagcca gcaggttggc
      361 gagtgcctcg tcagaatagg acttgggcgt ggacgaggca aaaccggcgg cgggaaaggg
      421 gttgagcgaa gcaatgaggc cgagtgcggc ttcgagtgtg ccgctgtcgg tttggggcag
      481 gcgtttttta ttcgcgccgg ggtttggctg cggttgccgt caatgctgtc gagggcgttt
      541 cggacgatat gcagggcggc gggtttggca gcacattcgc ccgatctttc tatctgcaat
      601 atcaacgatt cggtcacatc ggcggcggcc atgcctgccg gatcgaattt tttcaatgcg
      661 gtcagggctt gtttcagcat tgcttcatcc aacatccact ccaagggcgt atggtccagg
      721 atgtcttcga tgctgtcggt caggtagccc tgctcgtcaa ggaaatcgat aaggatgtgg
      781 acgcaggcgg cttcttggtc ggaaaggggg tgttcgcacg cttgcgcgtg caggtattgc
      841 ttgaaatctt cttcgccggc gatgttggac agcatatctt cgccttcgtc tccgccgatt
      901 tgacgcgcgg gcgcggtgta atggctgaat tcggcatcgg aaaattcatc cgtttctttg
      961 cgttcgagca gggggttgtc cgacggccag ttttcgacct cgcgttcaag ttcgataccc
     1021 ggcatctgca atacgcgcaa agattgttgc agccgctggt cgagctgctg ggtctgtttg
     1081 agctttattc cgattaaggt aatgataatg cggtgaaaat tgttattttc agcctgtcgg
     1141 cgcaaaaaaa tgccgcaaag cgtcattgca ttataaatgg ttttaatgag cgggttcgga
     1201 ttccgtccga taacaaaaaa caaacgaaaa tggaaacttg attgcttata acaatattaa
     1261 atcaatttca tagttttaat aacgaaaatc ttggcgtata atcgcatcca tagtttttgc
     1321 aaaaggaaaa taaaatgtcg attcaagaaa tttattacaa tcaagaaacc ggttacgaat
     1381 acgctttccg ccaaatcgta ctgtaagccg tttgcccccc atttgaccaa cctgagaaaa
     1441 ggaacaagag cgatgactac ctccaaatgc cccgtaaccc atctgaccat gaacaacggc
     1501 gcgcccgttg ccgacaatca aaacagcctg accgccggcc cgcgcggccc cctgctgacg
     1561 caagatttgt ggctgaatga aaaactcgcc gacttcgtgc gcgaagtcat ccccgaacgc
     1621 cgtatgcacg ccaaaggttc gggcgcgttc ggtacgttta ccgtaacaca cgacatcacc
     1681 aaatacaccc gcgccaaaat tttcagcgaa gtcggcaaaa aaaccgagat gttcgcccgt
     1741 ttcaccaccg tggcaggcga gcgcggcgca gccgatgcgg aacgcgacat ccgcggtttt
     1801 gcgttgaaat tctataccga agaaggcaac tgggatgtgg tcggcaacaa cacgcccgtg
     1861 ttcttcctgc gcgacccgcg caaattcccc gacctgaaca aagccgtcaa acgcgatccg
     1921 cgcaccaata tgcgttccgc cacaaacaac tgggacttct ggacgctgct gcccgaagcc
     1981 ctgcaccaag ttaccatcgt gatgagcgac cgcggtattc ccgccagcta ccgccatatg
     2041 cacggcttcg gttcgcatac ctacagcttc tggaacgaag cgggcgaacg tttttgggtg
     2101 aaattccatt tccgcagcca gcaagggatt aaaaacctga ccaacgaaga agctgcaaaa
     2161 atcatcgccg acgaccgcga aagccatcag cgcgacctgt acgaagccat cgagcgcggc
     2221 gagttcccga aatggacgat gtacatccaa gtcatgcctg aagccgatgc cgaaaaagtg
     2281 ccttatcatc cgtttgactt gaccaaagtt tggccgaaaa aagactatcc gctgattgaa
     2341 gtgggcgaat tcgagttgaa ccgcaatccc gaaaacttct tcgccgatgt ggaacaatcc
     2401 gccttcgcac cgagcaacct cgttcccggt atcggcgcga gcccggataa aatgttgcaa
     2461 gcccgtttgt tcaattacgc cgacgcgcaa cgttaccgct tgggtgtgaa cttccgccaa
     2521 atccccgtca atcgtccgcg ttgccctgtt cacagcaacc agcgcgacgg acaaggccgc
     2581 gccgacggca actacggcag cctgccgcac tacgaaccca acagcttcgg ccaatggcag
     2641 caacaacccg acttcgccga accgcctttg aaaatcaacg gcgacgcggc acactgggac
     2701 taccgccaag acgatgacga ctacttcagc caaccgcgcg ccttgttcaa cctgatgaac
     2761 gacgcgcaga aacaggcttt gtttgacaac accgccgcag ccatgggcga tgcgcccgac
     2821 ttcatcaaat accgccatat ccgcaactgc taccgttgcg acccggcata cggtgaaggc
     2881 gtagccaaag ccctcggact gaccgtcgaa gacgcacaag ccgcccgcgc gaccgatccc
     2941 gcactgggtc aggccggttt gctgtaaggg ggcattatgt ggatggaaat tgaagaaatc
     3001 ctgtcccgcg ccgtccgata ccggaaataa ccgggcataa aaatgccgtc tgaaacgtcg
     3061 tttgaccgtt tcagacggca tttccacacc tcgccccgcc gtttcagtgg gcgtttttta
     3121 ttatagtgga ttaaatttaa accggtacgg cgttgcctcg ccttgtcctg atttttgtta
     3181 atccactata aacgcaaaat atcccgtcat tcccgcacca tccccaccgg ttcaaaccgg
     3241 cacgaaaact tttccgcgtc attcccgcga aagtgggaat ccggaacgta aaatctaaag
     3301 aaaccgtttt atccgataag tttccgtgcc gacggggctg gattcccact ttcgtgggaa
     3361 tgacgggatt tctgtttttg attttttgtt tttgcgggaa taacgggatg cgggttttcg
     3421 tgcggcgttt ttcgcgcttt gctgttttgt tataatccgc cctttttaag gatgggtgcg
     3481 gtatgagtct ttacgctttg ctcttggttg cgctgggaat gtcgatggat gcgtttgccg
     3541 tcgcgctggc aaagggtgcg gcggtcagga tgcccccgcg caaaattgca gcaacggctt
     3601 tggtgttcgg tacggttgaa gcgttcatgc cgctggcggg ctgggtaggc ggtttttatg
     3661 ccaagccgtt tatcagcgaa tgagaccatt gggtggcttt tgtcctgttg ggcggactgg
     3721 gtctgaaaat gatgcgcgaa gggttgtccg gcgaggcgga agatgtgcgc gaaagcaaac
     3781 aggaaagcct atggatgacg gttttgactg cttttggaac cagtattgat tccatgatcg
     3841 tcggggtggg cttggcgttt atggaggtaa acatcgcctt tgccgccgcc gtaatcggta
     3901 tggcggctac ggtaatggtt accatcgggc tgacggcggg aaaggctttt ggcgtattgt
     3961 tcggcaggcg tgcggaattt gccggaggtt tggtgttgat tgccatcggt acatggacgc
     4021 tcttatcgca tttgggtttg attcaatgat gtcggaaaat aaaatgccgt ctgaagcttt
     4081 accggtttca gacggcattt tgttaaactt gattctctat cggtattatt gtgtaaagct
     4141 attgaaaata aaatattgat tttccgcaaa gatggctaaa ggtgcgatag gtacactgaa
     4201 gaaataatcg gcagaacagg tttgcctgcc ggtttgaaca ataattttaa cctcccataa
     4261 ggaatacacg atgtctttca aactccgtta cctcgcatca gtattggcat tgtcttcact
     4321 gttggcggca tgcggcggtc aggaaaagtc tgcggcaggc gatgcttctc ctgcttccga
     4381 gaccgaagcg gcttcccaag tacaggcctc ggaagccgtt ccttccgctt catcggcctc
     4441 gcccgaagac caagaccttt tgaaacgcgc gcaaggtgta ttccaacctt tgccgactgt
     4501 cgaagaaatg cagaaaatcc gtccgtttac cgaagaacag gtcaaactcg gacaccaact
     4561 ctggtatgaa ccacgccttt ccaaaggcaa taccgtaagc tgcaactctt gccacaacct
     4621 tgcttccgcc ggtgtggaca atatgccgac cagtcagggg cacaaagggc agttcggcgg
     4681 acgcaactcg cctaccgcat tgaatgctgc gctgctgggc agccagtttt gggacggacg
     4741 tgccgccgat gttgaagaac aggctggcgg gcctttggtg aatccggtgg aaatggcgaa
     4801 tgattcgcaa gaggcggctg cagccaaaat cgccaaagtt cccgaatatc aagaaatgtt
     4861 taaaaaagct tttcctgaag acggcgcggt ttcgtttaaa aacatcacta ccgcattggg
     4921 tgcgtttgag cgtaccctgc tgacgccgac caaatgggac gaatacctca aaggcaacgt
     4981 caacgccctg agcgaacagg aacgaaaagg cgtgcgcgcg ttcatggaca acggctgtat
     5041 tgcctgccac aacggtgtca accttggagg cacgaccttc cagaaattcg gtctggtcca
     5101 agggccgtat tggaaattca ttgaagatcc gaaacgcgac aaaggccgtg ctgacgtaac
     5161 caaaaaaacc gaagacgaat ttttcttccg tgttccgggg ttgcgtaacg tggctaaaac
     5221 ttatccgtat ttccacaacg gcagcgtgtg ggagctggat aaggcagtta ccatcatggg
     5281 taaggcgcaa ttgggtaaag acattccgaa agaagatgtg gataacatcg tcgtattcct
     5341 gaatgcactt tccggcaatg tttccgaatc agcgcgcacg atgccggaac tgcccctgac
     5401 cgcaccgatg gaatctaagc cggacaacaa ataatccggc cggtacgcaa aaatgccgtc
     5461 tgaaacggga atctgtttca gacggcattt tatatttcgt cgcgtcagat acgcagggta
     5521 tgccgtaggg tgtgcttcag ccagccgttt ccaccaattc aaccaaccat gtccgggtct
     5581 tatgcgtttt tcacgaatgc gcggcgtagg cggagggatt ggtgggctga agcctaattc
     5641 aatggctgcg ttgtgtaagc catgatgcgt ttggataatt aatcaatatt cttgcgctac
     5701 cattaccatc ggctttgggg gtttgtactt cggcattatg ataatcaata gcctgtttgg
     5761 attgtattcc cgaatccgac agttttagat tataaaaagg ccgtctgaaa aattttcaga
     5821 cggcctttta tagtaaattt caaataaaaa tatctttgtc gtcattccca cgcaggcggg
     5881 aatccagact tgtcggcaca gaaacttatc gggtaaaacg gtttcttcaa ttttatgttc
     5941 cggattcccg cctgcgcggg gataacgatt caggtatttc tacatcgaat ccgcttttta
     6001 tatcaacctg actatcaagc cgcgttgtcg aagccgctgt ggcgcagcag tgcgtctatg
     6061 ctcggttcgc gtccgcggaa ggccttgaag gattccgccg cgctgcggga gccgccgacg
     6121 gcgaggattt cctgccagaa gcgtttgcct gtggcggcaa cgtcgtcgct ttcttcaaag
     6181 gcggcgtagg cgtcggcgct gaggacttcc gcccatgcgt agctgtaata gcctgcggag
     6241 tagccgccgg cgaagatgtg gccgaagctg ttggcgaagc ggttgtattc gggcggttgg
     6301 atgacggcga cttctttgcg cacgctgtct aaaacctgct gccagttttt cagacggcct
     6361 tcgttgtctt cgctgtaaat ggtcatgtca aagagggcga attccatttg ccggacgagg
     6421 aacatgccgc gttggaagtt tttggcggcg agcattttgt cgaagagttc tttcggcagg
     6481 ggctcgccgg tttcttcgtg ggcggacatt tgtgccaata cattgtattc ccaaacgaag
     6541 ttttccataa actggctggg cagttcgacc gcgtcccatt ctacgccgtt gatgccggac
     6601 acgcccagtt cgtccacttg ggtaagcagg tggtgcagtc cgtggccggt ttcgtggaag
     6661 agggtgagga tttcgtcgtg gcttaaacgc gcttctttgc cgccgacggg cggggcgaag
     6721 ttgcagacga ggtaggcggt gggcagttgc agcgtgccgt cggcaaagcg gcggcggcct
     6781 ttgtagtcgt tcatccacgc gccgccgcgt ttgccttcgc gtgcgtacaa atccatataa
     6841 acgccgccga tggttttgcc gttttgctcc agctcgaagt agcgcacgtc tttgtgccag
     6901 acggggacgg ttttttcggc aaatccgatg ccgtagagtt ttttgatttg ggcgaacagt
     6961 ccgtttaata ctttgccgac ggggaagtat tttttgactt cggtttcgct gaatgcgtat
     7021 ttggcttcgc gcagtttttc gccggcgtag ctcaagtccc acggctgcgg gtcggcgaga
     7081 ccgaggtgtt cgcgggcgaa ggctttgact tcggcgaggt ctttttcggc gtagggtttg
     7141 gcgcggcggg cgaggtcgtg caggaagttt aaaacctgtt cgggcgtgtc cgccattttg
     7201 gttgccagcg acaattcggc gtaattttta aagccgagca gtttggcggt tttcaatgcg
     7261 ttttcgagcg tgcggtcgat gttggcggtg ttgtcgaatt tgccgtcgtt tgaaagttcg
     7321 ctggcacggg taacgtaggc gcggtagatt tgttcgcgca gttcgcggtt gccggcgtat
     7381 tggatgacgg caaggtagtg cggaatctgc aagccgattt tgtaacctgt tttgccttcg
     7441 ctttgcgcgg cggcggcaaa catcgcgagc gcgtcttcgg gaatgccggc aagcggtgcg
     7501 gcatcgtcaa agtaaatgcc gaacgcgtcg gtcgcgtcta ggacgttttg ggagaatttg
     7561 gcggaaagtt gcgcgccttc ggtttgcagt tttgccagtt ctgcctgctg ttcgggcggc
     7621 agttccgcgc cgctcaatac gaaatcgcgc aggtcgtgat cgagcttggt tttttgtgcg
     7681 ggggaaagcg ttgcaaattc gggggaattt ttgatggttt tgaagcggtt gtacagttcg
     7741 atgtcttgtc cgatttcggt gaagaagacg gtgatttcag gcatcagttc gttatagacg
     7801 gcgcgcagtt cgggcgtgtc gacgacggag ttgagatggg acacgacgcc ccaaatcctg
     7861 ccgacgcgtt cggtgatgcc ggtcagacgc tcgacggtgt tcgcccagcc ggtgtgcgtt
     7921 tgcgctttga cggcggcgat ttgtccgcgc gcttcggcga tggcggtttg gacggcgggt
     7981 ttgatgtctt cggttttgat ttgattaaaa cggggttctt cgcccaagtg gagcagtgcg
     8041 ttgtcaatca taagatgggt ttcctttgcg tgttgtgctt cagacggcat atcggcaata
     8101 tgccgtctga agccagaaat ggggccggcc ggggaaagtt aaagtgtttc ggagccggcg
     8161 ggatgattta acaggcggta ttgtacgcgc aaaaattcgt ccataaccgc ctgttggatt
     8221 tccagccgct ttgaaacggg ggaggcgaag cggacgatga tgcggtatgc cttgtcgtcg
     8281 tacggtacgc gggtaacgcg cggcctggcg gcgggcgtga taaacagttt ttccgcctgc
     8341 acgttttcca aataccgctg aatggcgggg atgtagggcg cgcacaaggg ctcgagtacg
     8401 gctttcagac ggcatacggc ttcatccgaa tccaaatgga tgggaacggg gatttcgacc
     8461 gtatggatga catagtcgcc caaaatattg tcgcggcgca cggggtggct caacaacagg
     8521 ctgttgggga aagaaacggt ggttcccgca agctgtccga ccaaggggtt cggaccgacc
     8581 tgcatcatca gcgtgttcaa cagattgatg tcgaccacgc gcccgcgcag gccgttgatt
     8641 tcgatatagt cgccgaccga gtattgctgg gtggcagacc ttaaaatact gcccgacaga
     8701 cacataatca gttcttttgt cgccacgacg accgccgccg ccaccgcaaa catcgacaac
     8761 gccagcgttt gaatttgcgc cgaccagata aatgccagcg aaaacagcac caaaagcagc
     8821 gttatattgc ggctggcaac caaaaaccgc cgcttgcttt cgatgccgaa atccggatgc
     8881 cgtctgaagt ggatattcaa cagaagggcg cgcgccagca gcaaagccgc gaccgccgcc
     8941 acggattcgg ccgcctccgc gcgtatcggg acggcatcgg gccaagtgtt caacatattc
     9001 catatttcca tttccgcgcc cctgtccgaa aagtataggg tgtagatttt agtggcaaaa
     9061 aaacgctttt gccactttgc cggcaaattc cgcgcccgta ccggccgcaa atgcgccgca
     9121 cgccctttca gacggcatcc ggatgcggat aacgctgaat caggcggcga aatcggctat
     9181 aatacacaat tatatgcgcc gtcccgcccg ctttttccac aaggtgcgga ctgttttggc
     9241 ggcatcgcaa aatcttttca aaatccggca aaaaatatga ctgaacaaaa acacgaagaa
     9301 tacggcgccg acagcatcca ggtgctcgaa ggcttggaag cggtacgcaa acgccccggc
     9361 atgtacatcg gcgacacgca ggacggcagc gggctgcacc atatggtgtt tgaagtattg
     9421 gacaacgcca tcgacgaagc actcgccgga cattgcgaca aaatcacggt aacgatacac
     9481 gccgaccatt ccgtcagcgt cgccgacaac gggcgcggta tgcccaccgg catccacccg
     9541 aaagaagggc gttccgccgc cgaagtcatc atgaccgtct tgcacgcggg cggcaaattc
     9601 gacaacaaca gctacaaaat ctccggcggc ctgcacggcg tgggcgtatc cgtcgtcaac
     9661 gcgctgtccg actgggtaac gctgaccatc taccgcgacg gcaaagaaca cttcgtccgc
     9721 ttcgtacgcg gcgaaaccga agagccgctg aaaattgtcg gcgattccga caaaaaaggc
     9781 acgaccgtgc gcttcctcgc cggcacggaa accttcggca atatcgaata cagcttcgac
     9841 atcctcgcca aacgtattcg cgaactttcg ttcctaaaca acggcgtgga catcgaattg
     9901 accgacgagc gcgacggcaa gcacgaaagc ttcgcccttt ccggcggcgt ggcgggcttc
     9961 gtgcaataca tgaaccgcaa aaaaacgccc ttgcacgaaa aaatcttcta tgcgttcggc
    10021 gagaaagacg gcatgagcgt cgaatgcgca atgcaatgga acgacagcta tcaggaaagc
    10081 gtgcagtgct tcaccaacaa catccctcag cgcgacggcg gtacgcacct gaccgcgctg
    10141 cgccaagtga tgacgcgcac catcaacagc tacatcgaag ctaacgaagt cgccaaaaaa
    10201 gccaaagtgg aaaccgccgg cgacgatatg cgcgaaggtt tgacctgcgt gttgtccgtc
    10261 aaactgcccg accccaaatt ctcatcccaa accaaagaca aactggtttc cggcgaaatc
    10321 ggccccgttg tcaacgaagt catcaaccaa gcactaaccg acttcctcga agaaaatccg
    10381 aacgaagcca aaatcatcac cggcaaaatc gtcgatgccg cccgcgcacg cgaagccgcc
    10441 cgcaaagccc gcgaaatcac ccgccgcaaa ggcgtgatgg acggcttggg actgcccggc
    10501 aaactcgccg actgccaaga aaaagaccct gccctgtctg aactctacct cgtcgagggc
    10561 gactccgcag gcggttccgc catgcagggc cgcgaccgca aattccaagc gattttgccg
    10621 ctcaaaggta aaattttgaa cgtcgaaaaa gcacgttttg aaaaaatgct cgccagccaa
    10681 gaggtcgcca ccctgattac cgcgctgggt gcaggcatcg gcaaagaaga gttcaaccct
    10741 gaaaaactac gctaccaccg catcatcatc atgaccgatg ccgacgtgga cggtgcgcac
    10801 atccgcaccc tgctcctgac cttcttctac cgccaaatgc ccgaactggt cgagcgcggc
    10861 tacatttaca tcgcccagcc gccgctctac aaagccaaat acggcaagca ggagcgttac
    10921 ctcaaagacg aactggaaaa agaccaatgg ctgctcggcc ttgccttgga aaaagccaaa
    10981 atcgtttcag acggccgcac catcgaaggc gcagaacttg ccgacaccgc caaacaattc
    11041 ttgttggcga aaaccgtcat cgaacaggaa agccgcttcg tggacgaact cgtcctgcgt
    11101 gccatgctgc acgcgtcgcc cattgatttg acgtcgtctg aaaacgccga taaagccgtt
    11161 gccgaacttt ccggtttgct tgacgaaaaa gaagccgccc tcgaacgcat cgaaggtcat
    11221 gaaggacacc ggttcatcaa aatcacgcgc aagctgcacg gcaacgtcat ggtcagctac
    11281 atcgaaccca agttcctcaa cagcaaagcc taccaaaccc tcacccaaac cgccgccgcg
    11341 ctcaaaggct tggtcggcga gggcgccaag ctctacaaag gcgagaacga gtacgacgcg
    11401 gacagctttg aaaccgcttt ggacatcttg atgagcgttg cccaaaaagg tatgtccatc
    11461 caacgataca aaggtttggg cgagatgaac cccgagcagc tttgggaaac cacgatggat
    11521 cccgccgtgc gccgcctgtt gaaagtgcgc atcgaagatg ccattgccgc cgacgaagtg
    11581 ttcgttaccc tgatgggcga cgaggtcgaa ccgcgccgcg cctttatcga aaacaatgcg
    11641 ctgattgcgc aaaatatcga cgcataagtg ccgttttaaa aaaggagacg ggcagcttgc
    11701 cgcgtctcct tttggtttgt caaacggaag ccatgccgtc tgaaaccgcc tttcggagca
    11761 aaaccatgat cagcattttt gatattttca aaatcggtat cggcccttcc agttcgcata
    11821 cggtcggccc gatgaaggcc gccgccgcct ttgccgcagg cttggacgcg caaaccgccc
    11881 gcatcgccat cgacatttac ggctcgcttg ccctgaccgg atacggacac ggcacatttg
    11941 acgcgctgat gctcggtttg gaaggcagcc tgccgcacga catcccgctt gccggcattc
    12001 ccgaacgcct cggacgcatc cgcacgcagc acatcctccg gctcaacggg caagaaatcc
    12061 gcttcatccc cgaccgcgac ctgaacatac gcggcaatca agtgctgccc aaacacccca
    12121 acggcctgcg atttaccgcc tatgcttcag acggcacggt attgaaggaa caggtttatt
    12181 attcggtcgg cggcggcttt gtcgttaccg aagaagactt cgagtggcag tcggaaacgg
    12241 aaaaagccgt tccttatcct tacaacagtt gtgccgggct gcttgcccga tgccgtctga
    12301 accggctcga catctccgaa gtcgtgttgg caaacgaagc cgcgcttgcc ggatgcggcg
    12361 aagccgaaat ccgccgccgg gttgccggtg ttgccgagat tatggaagag tgcatcaaac
    12421 gcggcttggg tgcggacggc gaactgcccg gcggattgaa cgtccgccgc cgcgccccgc
    12481 agcttgccgc caaactcaaa gtcctgcgcg aaaccgaaat cgtcaacacc cagctctggc
    12541 cgatggtgta cgccatggcg gtcaacgaag aaaacgccgc cggcggacgc gtcgttaccg
    12601 ccccgaccaa cggcgcggca ggcatcattc ccgccgtatt gcattatttc cgcaagttca
    12661 acccccacgc cacacaggaa cgtgtcgaaa acttcctgct caccgcaggc gcaatcggca
    12721 tcctctacaa aaccaacgcc tccatttccg gcgcggatgt cggttgtcag ggcgaagtcg
    12781 gcgtagcgtg ttcaatggcg gcgggtgcat acgccgaagt catcggcggc acgcccaaac
    12841 aagtggaaaa cgccgccgaa atggcaatgg aacaccattt gggcttaact tgcgaccccg
    12901 tcggcgggtt ggtgcaaatc ccctgcatcg aacgcaacgg catcgccgcc gaaaaagccc
    12961 tcaaactcgg cacgctcgcg ctcttggaag acggcacgga caaaaaagtc tcgctcgacg
    13021 aagtcatccg aaccatgctg caaaccggcc gggacatgaa ggcgacctac aaagaaacct
    13081 cgcttgccgg actcgccgcc acgctccgga aaaaagccgt ccccgtatcc gtgcgcgtgg
    13141 tcgagtgctg aggatggaca gaaacgaaaa tgccgtctga aaacagtttt tcagacggca
    13201 tttcatttat ttcttgcggg tttaccgctt caacgccaac gtcaacacgc ccgccgtaac
    13261 cagccccaag cctatccatt cctgcgtgtt cgggcgttcg tccaagaaaa ccaccgccat
    13321 caggaatgca aaccggcgtt gtttgcagca tccggcaaga atttttatag tggattaaca
    13381 aaaatcagga caaggcgacg aagccgcagg cagtacaaat agtacggaac cgattcactt
    13441 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtaccg
    13501 gtttttgtta atccactata aaaggtcttt tctaaaatat gcagatattc gaatgtcgaa
    13561 tttgccttat gattacggtt ttctgtctta tccgccttaa aacgccggta ttccgtttga
    13621 accaaattat atttgccgaa acactcaaaa atctccctta cctgcccgac agacatcaag
    13681 acttcgttgt tgtaactgag aaaaatatat tgaaagtccg catccctaat caaagcctca
    13741 aataccggaa ccacatccga ttttgatcaa gtccgcgcta aaacacatcg tacagcgcaa
    13801 aaccggatgg gaatggcaaa atccggaaac aaacattttt caaagcgtac aaacaaaaaa
    13861 taccaaccgg aggttggtat ttcttaact
//
LOCUS       DAXFWM010000064        13642 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000064 DAXFWM010000000
VERSION     DAXFWM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.064"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            193..1068
                     /gene="prpB"
                     /locus_tag="ACFI8B_001742"
                     /pseudo
     CDS             193..1068
                     /gene="prpB"
                     /locus_tag="ACFI8B_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
     gene            1153..2301
                     /gene="prpC"
                     /locus_tag="ACFI8B_001743"
     CDS             1153..2301
                     /gene="prpC"
                     /locus_tag="ACFI8B_001743"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC3834901.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLHLLHGKRLTESQVKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAECLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2453..3241
                     /locus_tag="ACFI8B_001744"
     CDS             2453..3241
                     /locus_tag="ACFI8B_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC3834902.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVALLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3318..5923
                     /gene="acnD"
                     /locus_tag="ACFI8B_001745"
                     /pseudo
     CDS             3318..5923
                     /gene="acnD"
                     /locus_tag="ACFI8B_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            6039..7201
                     /gene="prpF"
                     /locus_tag="ACFI8B_001746"
                     /pseudo
     CDS             6039..7201
                     /gene="prpF"
                     /locus_tag="ACFI8B_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            7513..8712
                     /locus_tag="ACFI8B_001747"
     CDS             7513..8712
                     /locus_tag="ACFI8B_001747"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC3834903.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNKKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            8823..9443
                     /locus_tag="ACFI8B_001748"
     CDS             8823..9443
                     /locus_tag="ACFI8B_001748"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC3834904.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(9467..9595)
                     /locus_tag="ACFI8B_001749"
     CDS             complement(9467..9595)
                     /locus_tag="ACFI8B_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834905.1"
                     /translation="MPPKPAFGRHFVCRFKRIFRPGAIRAGISGCRRQGARCRLNG"
     gene            complement(9605..10048)
                     /locus_tag="ACFI8B_001750"
     CDS             complement(9605..10048)
                     /locus_tag="ACFI8B_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC3834906.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            10222..10590
                     /locus_tag="ACFI8B_001751"
     CDS             10222..10590
                     /locus_tag="ACFI8B_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC3834907.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            10635..11789
                     /locus_tag="ACFI8B_001752"
     CDS             10635..11789
                     /locus_tag="ACFI8B_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC3834908.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(11847..12719)
                     /locus_tag="ACFI8B_001753"
     CDS             complement(11847..12719)
                     /locus_tag="ACFI8B_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC3834909.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            12804..13616
                     /locus_tag="ACFI8B_001754"
     CDS             12804..13616
                     /locus_tag="ACFI8B_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC3834910.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
BASE COUNT         3064 a         3736 c         3832 g         3010 t
ORIGIN      
        1 ctgcggtttt gccgcataat tccgaacggt ctgctgtttt tctttgattc gttttaaata
       61 tcaataagat aatttttccc atatattttt aatgattggg atgcccgacg cgtcggatgg
      121 ctgtgttttg ccgtccgaat gtgatggaag cctgtccata ctgaaaaaaa aagtctataa
      181 aggagaaata tgatgagtca acactctgcc ggagcacgtt tccgccaagc tgtgaaagaa
      241 tcgaatccgc ttgccgtcgc cggttgcgtc aatgcttact ttgcatgatt ggccacccaa
      301 agcggtttca aaaccatcta tctgtccggc ggcggcgtgg cagcctgttc ttgcggtatc
      361 cctgatttgg gcattaccac aatggaagat gtgctgatcg acgcacgacg cattacggac
      421 aacgtggata cgcctctgct ggtggacatc gatgtgggtt ggggcggtgc attcaatatt
      481 gcccgtacca ttcgcaactt tgaacgcgcc ggtgttgcag cggttcacat cgaagatcag
      541 gtagcgcaaa aacgctgcgg ccaccgtccg aacaaagcca tcgtttccaa agatgaaatg
      601 gtcgaccgca tcaaagctgc cgtagatgcg cgcgttgatg agaacttcgt gattatggcg
      661 cgtaccgatg cgctggcggt agaaggtttg gatgccgcta tcgaacgcgc tcaggcttgt
      721 gtcgaagccg gtgcggacat gattttccct gaagccatga ccgatttgaa aatgtaccgt
      781 caatttgcag atgcagtgaa agtaccagta ttggccaaca ttaccgagtt tggtgctaca
      841 ccgctttata cgcaaagcga gttggctgaa aacggcgtgt cactggtgct gtatccgttg
      901 tcatcattcc gcgcagcaag caaagccgct ttgaatgttt acgaagcgat tatgcgcgat
      961 ggcactcagg cggcggtggt ggacagtatg caaacccgtg ccgagctgta cgagcatctg
     1021 aactatcatg ccttcgatca aaaactggat aaattgtttc aaaaatgatt taccgctttc
     1081 agacggcctt tcaacaaatc cgcatcgacc gtctgaaaaa taaaacccat aaaaatacaa
     1141 aggagaaata ccatgactga aactactcaa accccgacct tcaaacctaa aaaatccgtt
     1201 gcgctttcag gtgttgcggc cggtaatacc gctttgtgta ccgttggccg taccggtaac
     1261 gatttgagcc atcgcggtta cgacattcta aatttggcac aaaaatgcga gtttgaagaa
     1321 gtcgcacact tgctgattca cggccatctg cccaacaaat tcgagctggc cgcttataaa
     1381 accaagctca aatccatgcg cggcctgcct atccgtgtga ttaaagtttt ggaaagcctg
     1441 cctgcacata cccatccgat ggacgtgatg cgtaccggcg tgtccatgct cggctgcgtt
     1501 catcccgaac gcgaaagcca tcccgacagc gaagcgcgcg acatcgccga caaactgatc
     1561 gccagcttgg gcagcatcct gctgtactgg tatcaatatt cgcacaacgg caaacgtatt
     1621 gaagttgaaa gcgacgaaga gaccatcggc ggccacttcc tgcacctgtt gcacggcaaa
     1681 cgcctgaccg aatcgcaggt aaaagccatg cacgtttcgc tgattctgta tgccgaacac
     1741 gagttcaacg cctccacctt tactgcccgc gtgattgccg gtacgggttc ggatatgtat
     1801 tcctgcatca ccggcgccat cggcgcgctt aaaggtccga aacacggcgg tgcgaacgaa
     1861 gtcgcctacg acatccaaaa acgctaccgc aacgccgacg aagccgacat ccgcgaacgc
     1921 atcggccgca aagaaatcgt gatcggtttc ggccatccgg tttacaccat ttccgacccg
     1981 cgcaacgtcg tgattaaaga agtggcgcgc ggtttgagcc gggaagcggg cgacatgcgc
     2041 ctcttcgaca tcgccgaatg cttggagagc gtgatgtggg aagagaaaaa aatgttcccg
     2101 aatctggact ggttctctgc cgtttcctac caaaaattgg gcgtaccgac cgctatgttc
     2161 acaccgctgt tcgtgatttc ccgtacaacc ggttggagcg cacacgtcct cgagcaacgc
     2221 aaagacggca aaatcatccg tccgagcgca aactacaccg gccctgaaga tttggcgttt
     2281 gtggagattg aagaacgata attgaagaat gcaatagcag tttgttcttt atttcggtat
     2341 gcaaagctga ggatttcaga cgaccttgcc ttattggaaa ggttgtctga aataagttta
     2401 atctaatagg agaagataat cctgtattgg cgcaagtaac aggataagaa acatggaaga
     2461 tttatacata atactcgctt tgggtttggt tgcgatgatc gccggattta tcgatgcgat
     2521 tgcgggcggg ggtggtttga ttacgctgcc tgcactcttg ttggcaggta ttcctcccgt
     2581 gtcggcaatt gccaccaaca agctgcaagc agccgctgct acgttttcgg ctacggtttc
     2641 ttttgcacgc aaaggtttga ttgattggaa gaaaggtctc ccgattgccg cagcatcgtt
     2701 tgcaggcggc gtggtcggtg cattatcggt cagcttggtt tccaaagata ttttgctggc
     2761 ggtcgtgccg gttttgttga tatttgtcgc gctgtatttt gtgttttcgc ccaagctcga
     2821 cggcagtaag gaaggcaaag ccagaatgtc tttttttcta ttcgggctga cggttgcact
     2881 gcttttgggt ttttacgacg gtgtgttcgg accgggtgtc ggctcgtttt ttctgattgc
     2941 ctttattgtt ttgctcggct gcaagctgtt gaacgcgatg tcttacacca aattggcgaa
     3001 cgttgcttgc aatcttggtt cgctatcggt attcctgctg cacggttcga ttattttccc
     3061 gattgtggca acgatggcgg tcggtgcgtt tgtcggtgcg aatttaggtg cgagatttgc
     3121 cgtccgcttc ggttcgaagc tgattaagcc gctgctgatt gtcatcagca tttcgatggc
     3181 tgtgaaattg ttgatagacg agagaaatcc gctgtatcag atgattgttt cgatgtttta
     3241 aaccctttca gacgactcct tcaaaacatc ggctgaaacc caaaccacaa gaaaaataga
     3301 tccacaggag aaccgacatg actgccaacc aaagttaccg caaaccgctg cccggtacgg
     3361 atttggaata ctacgacgcg cgtgcggcgt gtgaggacat caaacccggc tcttacgaca
     3421 agctgccgta cacgagccgc attttgtctg agaacttggt caaccgcgcg gacaaagtcg
     3481 atttgccgat gctgcaaagc tggctgggcc agttgataga agggaagcag gaaatcgatt
     3541 ttccgtggta tcccgcgcgg gtggtgtgcc acgatattct ggggcagacc gcgctggtgg
     3601 atttggcagg cttgcgcgat gcgattgccg aaaaaggcgg cgatcctgcc aaagtgaatc
     3661 cggtggtgca gacgcagctt atcgtcgacc actctttggc ggtggagtgc ggcggttacg
     3721 atcctgatgc cttccgcaaa aaccgcgaaa tcgaagacag acgtaacgaa gaccgtttcc
     3781 acttcatcaa ctggacaaaa accgcatttg aaaatgtgga cgtgattccg gcgggcaacg
     3841 gcatcatgca ccaaatcaat ctggaaaaaa tgtcgcccgt cgtccaagtc aaaaacggcg
     3901 tggctttccc cgatacctgc gtcggcacgg actcgcatac gccgcacgtt gatgcgctgg
     3961 gcgtgatttc cgtggcgtgg gcggtttgga agcggaaacc gtgatgctgg gacgcgcgtc
     4021 catgatgcgc ctgcccgata ttgtcggcgt ggagctgacg ggcaaacggc aggcgggcat
     4081 tactgccaca gacatcgtgt tggcactgac cgaattcttg cgtaaagagc gcgtggtcgg
     4141 ggcgtttgtc gaatttttcg gcgagggcgc gagaagcctg tctatcggcg accgcgcgac
     4201 catttccaac atgacgccgg agttcggcgc gactgccgcc atgttcgcca tcgacgcgca
     4261 aactattgat tatttgaaac tgaccggacg tgacgacgcg caggtgaaat tggtggaaac
     4321 ctacgccaaa accgcaggct tatgggcagg tggcttgaaa accgccgttt atccgcgcgt
     4381 tttgaaattt gatttgagca gcgtaacgcg caatatggca ggcccgagca acccgcacgc
     4441 gcgttttgcc accgccgatt tggcggcgaa agggctggcg aagccttacg aagagccttc
     4501 agacggccaa atgcctgacg gtgcagtgat tattgccgcg attacttcgt gtaccaatac
     4561 ttccaacccg cgcaacgttg tcgccgccgc actgttggca cgcaatgcca accgcctcgg
     4621 cttgaaacgc aaaccttggg tgaaatcttc gtttgccccg ggttcaaaag tagccggaat
     4681 ctatttgaaa gaagcaggct tgttgcccga aatggaaaaa ctcggcttcg gtatcgtcgc
     4741 cttcgcatgt accacctgta acggcatgag cggcgcgctc gacccgaaaa tccaacaaga
     4801 aatcatcgac cgcgatttgt acgccaccgt cgtattgtca ggcaaccgca acttcgacgg
     4861 ccgtatccat ccgtatgcga aacaggcttt cctcgcttcg cctcctttgg tcgttgccta
     4921 cgcattggca ggtagcatcc gtttcgatat tgaaaacgac gtactcggcg ttgcagacgg
     4981 ccgcgaaatc cgcctgaaag atatctggcc gacagacgaa gaaatcgatg ccatcgttgc
     5041 cgaatatgtg aaaccgcaac aattccgcga catttatatc ccgatgtccg acaccggcac
     5101 agcgcaaaaa gcaccaagcc cgctgtacga ctggcgaccg atgtccacct acatccgccg
     5161 tccgccctat tgggaaggcg cactggcagg ggaacgtaca ttaagaggta tgcgtccgcc
     5221 ggcgattttg cccgacaaca tcaccaccga ccacatctcg ccatccaatg cgattttggc
     5281 cggcagtgcc gcaggtgaat atttggcgaa aatgggtttg cctgaagaag acttcaactc
     5341 ttacgcaacc caccgcggcg accacttgac cgcccaacgc gcaaccttcg ccaatccgaa
     5401 actgtttaac gaaatggtga gaaacgaaga cggcagcgta cgccaaggtt cgttggcacg
     5461 cgttgaacca gaaggccaaa ccatgcgcat gtgggaagcc atcgaaacct atatgaaccg
     5521 caaacagccg cttatcatca ttgccggtgc ggactatggt caaggctcaa gccgcgactg
     5581 ggcggcgaag ggcgtgcggc tggcgggtgt ggaagccatc gccgccgaag gtttcgagcg
     5641 catccaccgc accaacctca tcggcatggg cgtcttgccg ctgcaattca aacccggcac
     5701 caaccgccat accctgcaac tggacggtac ggaaacctac gacgttgtcg gcgaacgcac
     5761 accgcgctgc ggcctgaccc tcgtgattca ccgtaaaaac ggcgaaaccg tcgaagttcc
     5821 ggttacctgc cgccccgata ccgcagaaga agcattggta tatgaagccg gcggcgtgtt
     5881 gcaacgtttc gcacaagatt ttttagaagg aaatgcagcg taaggtaatc gggtatcgtc
     5941 aaaatgtccg ggctgcgctc aaaaaggttt tcagacggcc tcgaatgttt gagagaccgt
     6001 ctgaaaaacc atattaaaaa tacaaaggag aactcgaaat gccgcagatt aaaattccag
     6061 ccgtttacta ccgaggcggt acatctaaag gcattttctt caaacgcacc gatttgccgg
     6121 ccgctgcgca acaagaaggc gaggcgcgcg acaaaatcct cttgcgcgta ctcggcagcc
     6181 ccgaccctta cggcaaacag attgacggtt tgggcaacgc cagttcgtcc accagcaaag
     6241 ctgtgatttc ggacaaatcc gaacgcgccg atcacgatgt cgattacttg ttcggacaag
     6301 tttccatcga caaaccattt gtcgattgga gcggcaactg cggcaacctt accgccgccg
     6361 tgggcgcatt cgccatcgaa cagggcttgg tcgataaatc caaaatccct tcagacggca
     6421 tctgcacggt caaaatctgg cagaaaaaca tcggcaaaac cattattgcc catgtgccga
     6481 tgcaaaacgg atcagtgttg gaaacaggcg atttcgagct tgacggcgta attttccggc
     6541 agccgaagta caaatcgaat ttttagatcc agccgacggc gaaggcagta tgttcccaac
     6601 cggcaatttg gtcgacgaaa ttgatgtgcc gaatataggt cgtctgaaag ccacgctcat
     6661 caacgcgggc attccgaccg ttttcctgaa cgccgccgac ttgggctaca cgggcaaaga
     6721 gttgcaggac gacatcaaca acgatgccgc cgcgctggaa aaatttgaaa ccatccgcgc
     6781 atatggcgcg ctgaaaatgg gtttgatcag cgacgtatcc gaagccgccg cccgcgcgcg
     6841 cacgccgaaa cccgccttcg tcgcgcccgc cgccgattac accgcctcca gcggcaaaac
     6901 cgtaaacgcc gccgacatcg atttgccggt acgcgccctg agcatgggca aactgcacca
     6961 cgctatgatg ggcatcgcct cggtcgccat cgccgccgcc gtgctcggta cgctggtcaa
     7021 ccttgccgca ggcggcggaa cgcgtaaaga agtgcgcttc gggcatccgt caggtacgct
     7081 gcgtgtcggt gctgccgccg aatgtcagga cggacaatgg acggccgcca aagcggtcat
     7141 gagccgcagc gcacgcgtga ttatggaagg ttgggtgcgc gttcccgatg attgtttttg
     7201 aaatcatgta ggacgcacat tgctgtccgc tgtttcagac ggcatttgat taaggattac
     7261 cataaagccg aataacctca ccgccgtcat tcccacgcag gcgggaatcc gcagccgata
     7321 ttctgtttcc tgatttttat cagagcctgc gtgtgagcaa atatggattc ccgcctgcgt
     7381 gggaatgacg ctaggaaata cggcatacgc tttgcccaaa gaggccgtct gaaacacact
     7441 ccgcccaacg cccatccttt ttcagacggc cccgcaccaa aaaaacaacc acaaactaca
     7501 aggagaaaca tcatgtccga ccaactcatt cttgtcctga actgcgtcag ttcatcgctc
     7561 aaaggcgccg ttatcgaccg caaaagcggc agcgtcgtcc taagctgcct cggggaacgc
     7621 ctgactacgc ccgaagccgt cattaccttc aacaaagacg gcaacaaacg ccaagttccc
     7681 ctgagcggcc gcaactgcca cgccggcgcg gtgggtatgc tgttgaacga actggaaaaa
     7741 cacggactgc acgaccgcat caaagccatc ggccgccgca tcgcccacgg cggcgaaaaa
     7801 tatcacgagt ccgtcctcat cgaccaagac gtccttgacg aactgaaagc ctgcatcccg
     7861 ttcgccccgc tgcacaaccc cgccaacatc agcggcatcc tcgccgcgca ggaacacttt
     7921 cccggcctgc ccaacgtcgg cgtgatggac acctcgttcc accaaaccat gccggagcgg
     7981 gcctacactt atgccgtgcc gcgcgaattg cgcaaaaaat acgccttccg ccgctacggt
     8041 ttccacggta ccggtatgcg ttacgtcgcc cctgaagccg cacgcatctt gggcaaacct
     8101 ctggaagaca tccgcatgat tattgcccac ttaggcaacg gcgcatccat taccgccgtc
     8161 aaaaacggca aatccgtcga taccggtatg ggtttcacgc cgatcgaagg tttggtaatg
     8221 ggtacacgtt gcggcgacac cgatccgggc gtatacagct atccgacttt ccacgcaggg
     8281 atggatgttg cccaagttga tgaaatgctg aacaaaaaat caggtttccc cggtatttcc
     8341 gaacttccca acgactgccg caccctcgaa atcgccgccg acgaaggccg cgaaggcgcg
     8401 cgcctcgccc tcgaagtcat gacctgccgc ctcgccaaat acatcgcttc gatggctgtg
     8461 gcctgcggcg gtgttgacgc actcgtgttc accggcggta tcggcgaaaa ctcgcgtaat
     8521 atccgtgcca aaaccgtttc ctatcttgat ttcttgggtt tgcacatcga caccaaagcc
     8581 aatatggaaa aacgctacgg caattcgggc attatcagcc cgaccgattc ttctccggct
     8641 gttttggtcg tcccgaccaa tgaagaactg atgattgcct gcgacactgc cgaacttgcc
     8701 ggcatcttgt agccaaaaaa gggggcgagt ccgcaaaaat gccgtctgaa aggttttcag
     8761 acggcatttt gtttggcgtt tgggcgtaca atacggaaaa catgacgata aggaaacaaa
     8821 ctatggcaca atttttcgcc gttcatcccg acaatcccca agaacgcctc atcaagcagg
     8881 cagtcgaaat catcaatcaa ggcggcgtgg tggtttatcc gaccgattcc tgttacgcct
     8941 tgggttgcaa actcggcgat aaggcggcga tggaacgcat actctccatc cgaaaaatcg
     9001 atttgaaaca ccacctgacc ctgatgtgcg ccgatttgag cgagttgggc acatacgcca
     9061 aaatcgacaa cgtacagttt cgtcagctta aagccgccac gcccgggcct tataccttta
     9121 tcttgcaggc gacgaaggat gcgccggcgc gcgccctgca cccgaaacgc aaaaccatcg
     9181 ggctgcgtat tcccgataat gccgttgcac aagccctgct ggaggagttg ggcgggccgc
     9241 ttttaagctg caccctgatg ctgcccgaag acggcgaacc attgaccgac ccttatgaaa
     9301 tccgcgagcg tttggaacac gccgtcgatt tggtaattga cggcggctgg tgcggaaccg
     9361 atccgactac cgtcatcgac atgaccgacg gcacggaact ggtgcgccgg ggtttcggcg
     9421 atacggcggt gttcggtttg tagggaaacc gatgccgtct gaagcatcag ccgttcagac
     9481 ggcatcgcgc gccttgccgg cggcagcccg aaatgccggc gcgtatcgcg cccggtcgga
     9541 atatccgttt gaaacggcaa acaaaatgcc gtccgaacgc gggtttgggc ggcattttga
     9601 tgcgttatcg caccgcaatc gggattttcg attcataaag caggtcgtag gtcggcttgt
     9661 tgagcaggtc ttgcagcgtg aaaccgtcca gatacgtgaa aaacgacttc attgcaccgc
     9721 cgagtatgcc cgtcagccgg caggacggcg taatcaggca ttcgttgttc gggcccatac
     9781 actcgaccag ctgcatcggt tcgaggtggc ggacgaccgc gccgatattg atgcgttcgg
     9841 gcggcgcggc cagcctcagc ccgccgcctt tcccgcgtac gctgtgcaag aacccgcctt
     9901 tgaccagcgc ggtaaccact ttcatcaaat ggcttttgga aatgccgtag gtcgaggcga
     9961 tggtggcgat attgaccagc gcgtcgtcgt tgacggcggt gtagatgagg acgcgcagcc
    10021 cgtagtcggt atgttgggtc agatacatga ttttctcggt atggatcgtt attcttatcg
    10081 gtacggttta agtttcacgg aaaatacctt aatggttgaa accctgtccg tcggggcggt
    10141 agaatgtagc ctgtctgcgg cggtatgccg tctgaaacat ccgcgctacc gtttgataat
    10201 ttgttattgt aacctaaatt tatgaaaccg ttgaaacgac attccgccct tgtcgggctt
    10261 tcgcgcgaac accaccactc gctttccctg tgcgtgcgta tgttgcggac gcccggggca
    10321 gaccatcgtg ccgaactcga gctgcatttt gccgaactgg aaacccattt tcgcgaagaa
    10381 gaagccaagt ttgccccaat ttggcagaat gtcgcgcctg agttgaaaac ccgttttgaa
    10441 ggcgatcacg ccaaactgcg gcagatgatg gcaagccccg aatgcggtaa cgcggcgtgg
    10501 aataccgctt ttgccacaac cctgcgcgac cacgcgcgct ttgaagaacg cgagctgttt
    10561 cccgccgtcg aaccgttttt gccggcatga ttccgttttg cggcaaatat attaatgata
    10621 aacaaggaac acacatgaaa tttaccaaac atcccgtctg ggcaatggcg ttccgcccgt
    10681 tttattcact ggcggcgctg tacggcgcat tgtccgtatt gctgtggggt ttcggctaca
    10741 cgggaacgca cgagctgtcc ggtttctatt ggcacgcgca tgagatgatt tggggttatg
    10801 ccggtctcgt cgtcatcgcc ttcctgctga ccgccgtcgc cacttggacg ggacagccgc
    10861 ccacgagggg cggcgttctg gtcggcttga ccgccttttg gctggctgcg cggattgccg
    10921 cctttatccc gggttggggt gcggcggcaa gcggcatact cggtacgctg tttttctggt
    10981 acggcgcggt gtgcatggct ttgcccgtta tccgttcgca aaaccggcgc aactatgtcg
    11041 ccgtattcgc aatatttgtg ctgggcggta cgcatgcggc gttccacgtc cagctgcaca
    11101 acggcaacct aggcggactc ttgagcggat tgcagtcggg cctggttatg gtgtcgggct
    11161 ttatcggcct gattgggatg aggattattt cgttttttac gtccaaacgg ttgaacgtgc
    11221 cgcagattcc cagtccgaaa tgggtggcgc aggcttcgct gtggctaccc atgctgaccg
    11281 ccatactgat ggcgcacggc gtgatgcctt ggctgtcggc ggctttcgcg tttgcggcgg
    11341 gcgtgatttt taccgtacag gtgtaccgct ggtggtataa acccgtattg aaagaaccga
    11401 tgctgtggat tctgtttgcc ggctatctgt ttaccggatt ggggctgatt gcggtcggcg
    11461 cgtcttattt caaacctgcc ttcctcaatc tgggcgtaca tctgatcggg gtcggcggta
    11521 tcggcgtgct gactttgggc atgatggcgc gtaccgcgct cggtcatacg ggcaattcga
    11581 tttatccgcc gcccaaagcc gttcccgttg cgttttggct gatgatggcg gcaaccgccg
    11641 tccgtatggt tgccgtattt tcttccggca ctgcctacac gcacagcatc cgcacgtctt
    11701 cggttttgtt tgcactcgcg ctgctggtgt atgcgtggaa atacattccg tggctgatcc
    11761 gtccgcgttc ggacggcagg cccggttgag taaaaccgcc gcagatttcg ggtctgcggc
    11821 ggtttgcttt tcagacggca tggcggtcag ttgccgtcca gccagcggtc gcgcgtggtt
    11881 ttggcttctt caaaatagcg gtacagggct tcgcggtcgt cggcggtcag gatgtctgcc
    11941 aaaacgtcca actgtttgcc caagccttga accagttgca gcaggctgtc tttgttggca
    12001 aggcagatgt ccgcccacac ggcgggatgg ccggaggcga tgcgggtgaa gtcccgaaag
    12061 cccgtggcgg cgaatttcag atattcctgt ccgtcggggt ggtcgagaat ctggtggaca
    12121 taggcgaagg cggtcaggtg gggcatatgg gagacggcgg cgaaaaccgc gtcgtggtgt
    12181 tgcgcgtcca tcgtgaaaat gtccgcaccg accgcgcgcc acaggttttc taccaatgcg
    12241 atgccgtctg aatcttcgcc gccgtggggc gtgatgatga gttttctgtg gcggaacagc
    12301 ccgaactgcg cggcttgcgc accgcttctg tccgaaccgg caatcgggtg tgcggcgatg
    12361 cagcggtgca ggcggtcggg caggcagcgg cggaaggctt cgatgaccga agatttggtg
    12421 ctgccgacat cggaaatcca agtgtgttcc ggcaaaacgg ggcgcagcgc ggttaaaacg
    12481 gcgggaacgg tggcgacggg cgtggcaatc agtaccaagt ccgcaccgcc gatgctgtcc
    12541 gcgtcgatga cgacggaagc ccggttaatc acgccgcgtt ccaatgcacg ttcgaggttg
    12601 tcgcggtcgg tgtcgatacc ggtaacggtg cggacgagtc cctgcctttt gaggtcgaga
    12661 acgaacgaac cgccgatcag ccctacaccg atgagggcaa tatggttcaa aatgggcatt
    12721 tgtgtaaacg gttttcgcaa agtgccgcca tggtagccta tcggcggaat atgccgcaag
    12781 gtcggcagga aaaaggagaa gaaatggaca aaatcagagt tgccgccgtg cagatggtgt
    12841 cgggcgtgtc gccggaaacc aacgtcgccg ccatgaaacg cctggtcgca cgggcggcgg
    12901 agcagggtgc ggattgggtg ctgctgcccg aatattgggt gctgatgggc gcaaacgata
    12961 ccggcaaact cgcacttgcc gagcctttgg gcggcggacg ctttcagacg gcattgagcg
    13021 aaacggcgaa agaatgcggc gtggtgctgt tcggcggaac cgtgccgctg caaagccccg
    13081 aagcgggcaa ggtcatgaat actttattgg tgtacgggtg cgacggcgta aggacggggc
    13141 tgtaccacaa aatgcacctc ttcggttttt ccggtttggg cgaacgctat gccgaagccg
    13201 ataccatccg cgcagggcgg gaagtgccgc acttgtcggc agaaggcatg ccggtggcgg
    13261 caggtatttg ttacgatgtc cgctttcccg aatttttccg ccgccaactg ccgtttgacg
    13321 tattgatgct gccggcggca tttacccata cgacgggcaa ggcgcactgg gagctgctgc
    13381 tgcgcgcgcg tgccgtcgaa aaccaatgtt acgtcgtggc ggcggcacag ggcggtttgc
    13441 acgaaaacgg acggcgcacg ttcggacaca gtatgattgt cgatccgtgg ggcgacgtgt
    13501 tggacgtatt acccgaaggc gaaggcatcg ttacggcaga catcgatgcc aaccgcctga
    13561 acagcgtccg caaccgcctg cccgccttga aacaccgggt tttggatgcc gtctgaaggt
    13621 tcagacggca ttggaatcgg gg
//
LOCUS       DAXFWM010000065        13376 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000065 DAXFWM010000000
VERSION     DAXFWM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.065"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            177..926
                     /locus_tag="ACFI8B_001755"
     CDS             177..926
                     /locus_tag="ACFI8B_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3834911.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWREDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            1017..3380
                     /gene="pheT"
                     /locus_tag="ACFI8B_001756"
     CDS             1017..3380
                     /gene="pheT"
                     /locus_tag="ACFI8B_001756"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC3834912.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            3454..3756
                     /locus_tag="ACFI8B_001757"
     CDS             3454..3756
                     /locus_tag="ACFI8B_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC3834913.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            3740..4048
                     /locus_tag="ACFI8B_001758"
     CDS             3740..4048
                     /locus_tag="ACFI8B_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834914.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            4224..4709
                     /locus_tag="ACFI8B_001759"
     CDS             4224..4709
                     /locus_tag="ACFI8B_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC3834915.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            4901..5125
                     /locus_tag="ACFI8B_001760"
     CDS             4901..5125
                     /locus_tag="ACFI8B_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834916.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            5343..6644
                     /gene="bioA"
                     /locus_tag="ACFI8B_001761"
     CDS             5343..6644
                     /gene="bioA"
                     /locus_tag="ACFI8B_001761"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC3834917.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            6641..7288
                     /gene="bioD"
                     /locus_tag="ACFI8B_001762"
     CDS             6641..7288
                     /gene="bioD"
                     /locus_tag="ACFI8B_001762"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC3834918.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSYPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            7303..7779
                     /locus_tag="ACFI8B_001763"
     CDS             7303..7779
                     /locus_tag="ACFI8B_001763"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC3834919.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            7799..7894
                     /locus_tag="ACFI8B_001764"
     CDS             7799..7894
                     /locus_tag="ACFI8B_001764"
                     /inference="COORDINATES: protein motif:HMM:NF026993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold putative polymorphic
                     toxin"
                     /protein_id="HFC3834920.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            7903..8310
                     /locus_tag="ACFI8B_001765"
     CDS             7903..8310
                     /locus_tag="ACFI8B_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834921.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            8300..9190
                     /gene="ubiA"
                     /locus_tag="ACFI8B_001766"
     CDS             8300..9190
                     /gene="ubiA"
                     /locus_tag="ACFI8B_001766"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC3834922.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            9375..9824
                     /gene="ptsN"
                     /locus_tag="ACFI8B_001767"
     CDS             9375..9824
                     /gene="ptsN"
                     /locus_tag="ACFI8B_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC3834923.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            9828..10790
                     /gene="hprK"
                     /locus_tag="ACFI8B_001768"
     CDS             9828..10790
                     /gene="hprK"
                     /locus_tag="ACFI8B_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC3834924.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            10771..11625
                     /gene="rapZ"
                     /locus_tag="ACFI8B_001769"
     CDS             10771..11625
                     /gene="rapZ"
                     /locus_tag="ACFI8B_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC3834925.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            11706..12647
                     /gene="ylqF"
                     /locus_tag="ACFI8B_001770"
     CDS             11706..12647
                     /gene="ylqF"
                     /locus_tag="ACFI8B_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC3834926.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            12748..13350
                     /locus_tag="ACFI8B_001771"
     CDS             12748..13350
                     /locus_tag="ACFI8B_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC3834927.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPSTDEEEPETVPSDTLPN"
BASE COUNT         3361 a         3109 c         3641 g         3265 t
ORIGIN      
        1 attgactatt tttcttcaga aatagacgac tggttattgg caagcgagaa ggagttaaaa
       61 gattatgatg tatacctata caaattagta ctagatggac agcaatatgt tcagggaata
      121 agaaaatctg tattgtacga tttccttatg gaaaatcaga aagatagctg ataaaaatga
      181 aaaaatggac tagagatgaa accctggttg ccctttatct ttattacatc attccttttc
      241 aaaaggttag taaggacaat cctgttattc aagaatatgc tgaaatactg ggcaggacac
      301 cgtccgcact cggtatgaag ataggaaatt tgggtcgttt agatcctacg ctgaaagtca
      361 aaaatatttc gggattaagt aatggcagta aaatggatgt tgtcgtatgg aatgagtttt
      421 caggagattg ggagcaactt aataaagaat ttgagggagt catttcccaa taccaatcaa
      481 atgatgagaa tagtaatatt gaaatagaat cacctgagat tccaaaggga agggaaaggt
      541 ttgcaagaat atcagtacgc gtcaatcagg gctttttccg ttccagtgtt ttggcagcat
      601 acaataatca atgttgtatt actggattaa aacaacctga attgttagtt gctagtcata
      661 tcaaaccctg gagggaagat aaggataacc gtttaaaccc aaggaatggg ctatgtttga
      721 atgcgttaca tgacaaagct tttgataggg ggttgttagg aatagatgaa aatttcaaaa
      781 ttatattttc tccactactg gcaaaaacag aaggatttga tgatttattt aaaccatatg
      841 aaaatcggat gataagactg cctgaaagac taaacccaag tctggaattt ttaaaatttc
      901 acagagaaaa tatttttcag agttgagtaa atatcaacat taaccagaat tctaaaacag
      961 actgtctgaa cgaaaattga taaaacaaaa gttaattaat tgattgagaa cataacatgc
     1021 aattctccta ctcatggctg aaaacccaag ccaatcctga tctttctgcc gataaactgg
     1081 aacatctttt gaccatggcc ggtttggaag tggaagaaat cgatactgcc gcccctgctt
     1141 tcagtggcgt ggttgttgcc gaagtaaaat ctgttgaaaa acatcctgat gcagaccgtt
     1201 tgaacgttac tcaagttgat gccggtacgg gcgagttggt tcagattgtt tgtggtgcgc
     1261 cgaatgtcaa gccgggcatt aaagtgccgt gttcgttgcc ggttgccgtt ttgccgggca
     1321 attttaaaat taagccgact aaaatgcgcg gcgtaccatc aaacggtatg ctgtgttcga
     1381 ccaatgaact gggtttgcct gatgatggtg tagacggcct gcacattctg cctgaagatg
     1441 cgcctgtcgg taccaacatc cgcgaatact tggatttgga cgatatgctg tttacgttga
     1501 aaattacgcc taaccgcgct gattgcttga gtgttaaggg cattgcgcgt gaggtttctg
     1561 cgttgactca atgtgcgttt acgcctgttg aaattcagac ggcccctatc ggcagtgaga
     1621 aaaaacaggc tgtccgtatt gatgcaccgg ctgactgcgg ccgttttatc agccgcgtta
     1681 ttgaaaatgt caacgcgaaa gcggctactc ctgattggat gaaacaacgt ttggaacgca
     1741 gcggtatccg cagcatttcc gcattggtgg acattggcaa ctatgtgatg ctggaaatcg
     1801 gtcagcctat gcatgttttc gatgctgata agctgtcggg cagtttgatt gtccgccgtg
     1861 ctcaaaatgg cgagactttg gcgtgtctga atgagaagac ggttactttg gctgacaata
     1921 cactggtggt cgctgatgaa aaaggtgcgt tgagcttggc cggcttgatg ggtggcgcgg
     1981 cgagcgcggt ttcagacggc acgcaaaata tcgtgctgga agcggcttgg tttgagcccg
     2041 aaatcatcgt cggcaaatcg cgccaatacg gtttcggttc ggattcgtcg ttccgcttcg
     2101 agcgcggcgt ggattaccgt ttgcaggcgg atgccattga acgcgccacc gaattggttt
     2161 tgcagatttg cggcggtgcg gcaggcgaga tggtggaagc acaaggtaaa ttgcccgaag
     2221 ccaagcaggt cggattgcgt ttgggtcgtc tgaaaaccgt gttgggcgtg gacattcctg
     2281 ccgaacaggt ggaaaccatt ttgcaacact tgggtctgca gcctgagaaa acggcggaag
     2341 gcttccgcat taccgcgccg agcttccgtt tcgacatcga aattgaggct gatttgattg
     2401 aagaaatcgg acgcgtttac ggctatgaaa acatccccga cgattacacg tcaggccgtc
     2461 tgaaaatgtc ggaactgccc gaaacacgcc gtccgcgttt tgccgtttac aacgaaatgg
     2521 cggcgcgcgg ctaccgcgaa gtgatcagct atgccttcgt tgacgagcag tgggaactcg
     2581 attttgccgc caacgccgcc cccatccgcc tgcaaaaccc gctggcggcg caatatgccg
     2641 tgatgcgttc tacgttgatt ggcggcttag tggaaatttt gcaaaacaac ctgaaccgca
     2701 agcaaaaccg cgtgcgcgtg tttgaaatcg cctgcgtgtt cggcaaaggt tcagacggcc
     2761 ggtttgtcca aaacgaacgc atcggcggtc tgtggtacgg cgcggcgatg cccgaacaat
     2821 ggggtgagaa aacgcgcaat gcggattttt acgacatcaa ggcggacgtg gaaaatctgt
     2881 tgaaaaacaa agcggtcgag ttcgttaaaa ccggacatcc cgccctgcat cccggacgtg
     2941 ccgccaatat cgtttcagac ggcaaagtca tcggctttgt cggcgaactg catccgaaat
     3001 ggctgcaaaa atacgacctg ccgcaagcgc cgctggtatt tgaaatcgat atggcggctg
     3061 tgttggaatg cgggaaaacg cgctatcggg ccgtatcgaa attccagccg gtgcgccgcg
     3121 atttggcgtt tgtgatgccg gaagttatga atcatgatga tttactgctt gtcttgaaag
     3181 gcgcagcaaa caagttggta caggaaatca gcgtgtttga cgtgtatcgc ggcacgggtc
     3241 tgcccgaagg gatgaagagc gtggcggtta aagtgatttt gcaggatatg gaaaacacgc
     3301 tgacggatga ggcggtcgag ccgcttattg gaaaactgat tggcgtggca acggaggcag
     3361 gggcgcggct tcgcagttaa aaaataaata ttcgcttgaa ttttaaataa aaattggtaa
     3421 taatccacaa ctgttacaac agaaggtaat catatgacac taactaaagc agaactggcc
     3481 gatattttgg tggataaagt cagcaacgtc accaaaaacg atgccaaaga aattgtcgaa
     3541 ctcttttttg aagaaatccg cagcactttg gcaagcggcg aagaaatcaa aatttccggt
     3601 ttcggaaatt tccagttgcg cgacaagccg caacgcccgg gccgtaatcc gaaaaccggc
     3661 gaggaagtgc cgattaccgc ccgccgcgtg gtaactttcc atgccagcca gaaactcaaa
     3721 ggcatggtgg aacactacta tgacaaacaa cgttgatttg acgattcctg caaaacgcta
     3781 tttcacgctg gacgagttgt gcggactgtt gcaaatcagc ccctatggtt ttgcgcaatg
     3841 gcagcatgat cacggtgtgg ttgtcggtta cggcggagaa cgctacaccc gtttggatgt
     3901 ggtgaaactg ttgaaactga agagcacgtt tgcaccgtat gcagaaggtg cggaatcggg
     3961 ttcggacggc aaccgtccgg ttacgcttca ggaaatcgga gacggtctga aagacctgtt
     4021 ggaggatttg gataaggagt tgtgctgatt tgagaccggt tgcaggtatg cagccggttt
     4081 tgttttacac gctaaaaata attattgcgt gatttcacat tgtttcggct tgaagcacat
     4141 ggttttgtaa tcatttacag gcagctcgct tggaatcctg ttcgggcggt ttgctgttta
     4201 cttaaatata aggatgacgg tcaatgagat ttttcggtat cggttttttg gtgctgctgt
     4261 ttttggaaat tatgtcgatt gtgtgggttg ccgattggct gggcggcggt tggacgctgt
     4321 ttctaatggc ggcaaccttt gccgccggtg tgctgatgct caggcatacg gggctgtccg
     4381 gtcttttatt ggctggcgcg gcggtaaaaa gtagtgggaa ggtatctgtt tatcagatgt
     4441 tgtggcctat ccgttatacg gtggcggcgg tgtgtctgat gagtccggga ttcgtatcct
     4501 cggtgttggc ggtattgctg ctgctgccgt ttaagggagg ggcagtgttg caggcaggag
     4561 gtgcggaaaa ttttttcaac atgaaccaat cgggcagaaa agagggattt ttccacgatg
     4621 acgatattat cgagggagaa tatacggttg aagagcctta cggcggcaat cgttcccgaa
     4681 acgccatcga acacaaaaaa gacgaataaa tatgaatgga atgccgtctg aaggttcaga
     4741 cggcattttt ccggtttgaa aatatagtgg attaacaaaa accagtacgg caaggcgagg
     4801 caacgccgta ctggtttttg ttaatccact ataaatttaa tccactataa aatagggctg
     4861 taaccttcaa tcggaatttg ttgcctgcgg gatatacggt atgagtgttt gggatatatg
     4921 ggacaggatg gtggaaatct atcataagta taagaagccg tgcctggttt tagcagtgga
     4981 ttttgtgatg ggtatggtat tcatagagcc gaatgaggag ccgtgcatcg gcagatgcta
     5041 tgcgcctatg ccggagtccc ctgattttgc caacgctgtt gcgatggctg ttgctatgat
     5101 ctgtatcgta tttatctatg attgattgaa gcgttgggcg gaggctgtgt gaaacggtat
     5161 tgggcgttgg gtcgtctgat tccaatcagg cttgaggaat gcgaaacggt gtgcgcttat
     5221 gttgcggacg atttgtttcg cggtttttcg cccgaaacgg atggaaaggt gtgggaagcg
     5281 gtctgtcgga gtagaatacg ctttttgcgt ttgaatgcag taataagaaa agagagaaac
     5341 ttatgccgtc tgaacatcaa cacacatcat cattacttaa tttcgaccgt acccatctgc
     5401 tccatcccta tacttccatg accgatccgc ttcccgttta tcctgtcaaa cgtgcagaag
     5461 gggtgtttat cgaattggcg gacggcacgc ggctgattga cgggatgtcg tcttggtggt
     5521 gtgcgataca cggttacaat catcccgttt tgaatcaggc ggttgagaat cagatgaaac
     5581 aaatggcgca cgtgatgttc ggcggcctga cgcacgagcc ggcggtggag ctgggcaagt
     5641 tgttggtcgg gattttgccg caggggctgg accgtatttt ttatgcggat tcgggttcgg
     5701 tttcggtaga agtcgcgttg aaaatggcgg tgcaatacca gcaggcgcgg ggtctgacgg
     5761 cgaagcagaa tattgcgacg gtacggcgcg ggtatcacgg cgatacttgg aacgcgatgt
     5821 ccgtttgtga tccggaaacg gggatgcacc atattttcgg cagcgcgttg ccgcagcgtt
     5881 attttgtcga taatccgaaa aaccgtttcg acgatgaatg ggacggggcg gatttgcagc
     5941 ctgtccgcgc cttgtttgaa gcgcatcatg tggatattgc cgcctttatt ttagaaccgg
     6001 tcgtgcaagg cgcgggcggc atgtattttt atcatccgca gtatcttcgc ggcttgcgcg
     6061 atttgtgcga cgaatttgat atcgtgttga tttttgacga aatcgccacc ggattcgggc
     6121 gcacgggcaa gatgtttgcc tgcgaacacg cggaggtcgt gccggatatt atgtgtatcg
     6181 gcaagggttt gagcggcggc tatatgacgc tggcggcggc aatcacttcg caaaaagtta
     6241 ccgaaacgat ttcacgcggc gaagcgggcg tgtttatgca cggcccgaca tttatggcga
     6301 acccgctggc gtgtgccgtt gcctgtgctt cggtcaagct gcttttatcc caagactggc
     6361 aggcaaatat ccgccgtatc gaaagcatct taaaaggccg tctgaaagcc gcgtgggaca
     6421 ttcgcggcgt gaaagacgtg cgcgttttgg gtgctatcgg cgtgatagag ttggaaaaag
     6481 gcgtggatat ggcgcgtttt caagcggatt gtgtggcgca gggcatttgg gtacgcccgt
     6541 tcggcaggct ggtgtatctg atgccgccct atatcatttc agacggcatt ttgaccaaac
     6601 ttgccgataa aaccgtgcaa atcttgaagg aacacagcaa atgaaaggcg tttacttcgt
     6661 cagcggtata gacacggaca tcggcaaaac cgtcgccacc ggtatgttgg caaaacaatt
     6721 gttgcagcag ggcaaaagcg tgattacgca aaagcccgtg caaaccggtt gccaagacat
     6781 cgccgaagac atcgccgtcc accgtaaaat catgggcata cccatgcagg aagctgatga
     6841 acagcgtctg actatgcctg aaatcttcag ctatcccgcc tcgccccacc ttgccgcccg
     6901 actggacggc aggggtttgg acttggacaa aatccgcacc gccacacaag aattggcggc
     6961 gcagtacgaa gtcgttttgg tcgaaggcgc gggcgggttg atggtaccac tgacggaaaa
     7021 gctgttaacc attgatcata ttcagcaaca ggcttatccc gtcatccttg ttaccagcgg
     7081 acggctggga agcattaatc acaccctgct cagtttcgtc gtgctcaaac aatacggcat
     7141 tcgcctgcac agcctcgtgt ttaaccacat ccacgacagc cgcgacgcac acgtcgccca
     7201 agacagcctg aactatctgc aatgccgtct gaaagccgat tttcccgaag cggaatggat
     7261 ggagttggca aaaacaggcg cggtataaag attgggaaag atatggaaca cctatttggg
     7321 aaatggctgc ccgacttgcc cgcccccgtt tcagacggca tcgacctgcc gatgagccgg
     7381 ttattgaaag cccggtcgct gaccgccgca ttgtgcgcct tgccgcatac attttcggtg
     7441 gaactgctga aactgggcga agcggaaacg gaatacggac ggcggagggt gcgcgatgtg
     7501 cttctgaaac tggacggcac tgctgttgtg caggcacgaa gcgcgtgcag cgtcggttcg
     7561 gcgttttggc aaaacatttt ggactgcggc acacgccctt tgggcgagcg tctgtttcaa
     7621 gccgatttgg aaggggcgcg ttcggcgttt gagtttgccg tttccagtga aggatgcgga
     7681 cggtactttg ccgcgcggcg ttctcggttt tcccatcaag gcgaggaaat gctgctgacc
     7741 gagtattttc tgcccgaact gaaacgcttt atcggataaa ataccgtttt ttcaagctgt
     7801 gcggcaacct gcatctaaac cacattttaa tataagagac tccaatttag atacaggaca
     7861 tgtcgatggt actcacggac actataattt ttagtaagaa atatgaatat aatagaaata
     7921 ataagtagga atcgttttct aaaacaaata tatcctagtg gcataatgga tatttcacta
     7981 gtctcttttt caactgactt gtctaattgt attttaacta tccgaacaag tacaaagcct
     8041 tctgtagaaa tcgaaaaatg ggggctgtgg ctaaaagatt atgatacagt tgaaattgaa
     8101 ttaagaaata gctttattaa aggaatgaaa tgtcaaaatt ggtcgcataa caatagaaat
     8161 atatgccaag tagaaataaa gaaccaagaa gatggtctaa aaataataag attttacgac
     8221 aataattcaa attggttatt ggaactagaa gtttatggat tagttttcca agggtgtaag
     8281 acttatatga aagagggtta tgaatcttaa atccccttta tttttacgac tgtccgatcg
     8341 tttggacgtt tatatcagac tgatgagggc ggacaagccc attgggacgc tgcttttact
     8401 gtggccgacc tactgggcat tgtggctggc ttcagacggc attcccgatt tggcggtatt
     8461 ggcggcgttt acaatcggca cgtttttaat gcgcagtgcc ggctgcgtca tcaacgactt
     8521 tgccgaccgt gattttgacg gtgctgtcga gcgcaccaaa aaccgtccgt tcgcacaggg
     8581 cagggtcaag aaaaaagaag ccctgctgct gacggcattt ttgtgtctgc ttgccgcatt
     8641 gtgcctgatt ccgctgaatc atctgacatg gctgatgagc ctgcccgcgc tgttccttgc
     8701 gctgacttac ccgtttacca aacgcttttt tccgattccc caattttatc tgggacttgc
     8761 cttttccttc ggcatcccga tggcgtttgc cgcagtgggc aacagcgttc ccgttgaagc
     8821 gtggatactc tttgccgcca atgttttatg gacactggct tacgataccg tctatgcaat
     8881 ggcggacaaa gaggacgatt tgaaaatcgg catcaaaacc tccgccgtca cgttcgggcg
     8941 ttacgacatc gccgccgtta tgctgtgtca tggcggcttt accctgctga tggcagtatt
     9001 gggtgcggtt atcggtgcgg catgggcata ttggacggca atccccatcg tcctgctgct
     9061 gcaataccgc caatatgccg ccatcaaaag ccgcgtccgg caaatctgtt ttgaaacgtt
     9121 tttggcaaac aacagaatcg gttgggtgtg gtttgccgct atttttgccc atacgttttt
     9181 cgcgaaataa ggcagggcaa tgccgtctga agaaccgtaa actgctttgg acggcatttc
     9241 tatctgtgcc gaaaagcgtt aaaatatgtt tttgaaacgc tgtgttatgt cagcccgtgc
     9301 cgtatgcggg attgagattt gccccggcag tcggtacaat ccttctgttt tgcgatgtct
     9361 gaaaagagaa gcttatgagc cttatcggcg aaattttgcc tttgtcccat attgttttgg
     9421 atatggaggt gggcagtaaa aaaaggctgt ttgaggaagc gggcctgctt ttggaacgcg
     9481 aatcctcatt gtcccatgct gatgttttcg aatgtctttt tgcccgtgaa aaactcggtt
     9541 cgaccggttt ggggcagggt gtcgccatcc cccacggccg ccacgcgagc gtgaagcagg
     9601 cgacgggcgc gttcatccgt acgcgcgaac ccgtcggatt cgacgcgcct gacggcaaac
     9661 cggtttcact ggtttttatc ttgctggtgc cggaaaacgc aacgggcgag catttggaag
     9721 tcttatccaa actggccggc aagttttccc aaaaaagcat cagagaatcg ctgatgacgg
     9781 ttgcttctgc ggaagaagcc cgcgccatcc tgaccgaaga ataaaatatg cccagtatct
     9841 ccgtccgccg cctgtttgac gacaaccaat acaagttgca acttgcctgg gccgccggca
     9901 attcgggtgc ggacaaccgt atcggcgtag aggcggacaa gcccgtcctc gccctggtcg
     9961 gacacctgaa tttcatccat cccaaccaaa tccaagtggt cggtttggcg gagtcggaat
    10021 atctgaaccg cctcgaatcg ggggagacgg gttatcagtt cggagacctg ttcgatattt
    10081 ccatgtcttt ggtcattgtg gcaaacgatt tgccggtttc cccggggctg cgcgactact
    10141 gccacaaaaa cgacatcccc ctgttgactt ccaaactcga aagcccttat ctgatggatg
    10201 tgttgcggat ttacctgcaa cgcaccttgg cagcatcgtc cgtcaaacac ggcgtatttc
    10261 tcgatgtgtt tgaaatcggc gtgctgatta ccggacattc tggtttgggt aagagcgaat
    10321 tggcattgga actgatttcg cgcggccata gcctgattgc cgacgatgcg gtcgagctgt
    10381 tccgcatcgg tccggaaaca ttggaagggc gttgttcgcc tatgctgcgc gattttttgg
    10441 aagtgcgcgg cttggggata ctcaatatcc gccatatttt cggcgaaact tccatccgcc
    10501 ccaaaaaaat cctgcaactc attatcaatt tggtcgaggc ggacgacgag tatatgaagc
    10561 agctcgaccg gttgagcatc cgcaccgaaa ccgaatccat cctcaacgtc aacgtccgtt
    10621 cggttacgct gcccgtcgcc gtcggacgca accttgccgt tttggttgag gcggcggtac
    10681 gcaattacat tttgcagttg cgcggcaaag acagtacgcg cgaatttttg gaacgccatc
    10741 agacgcaact taaagaaaac gaacagaatc atgaaaatcg tcctgattag cgggctttcc
    10801 ggctcgggca aatccgttgc actgcgccaa atggaagatt tgggctattt ttgcgtggac
    10861 aacctgccct tggaaatgct gccgtcgctg gtgtcgtacc atatcgaacg tgcggacgaa
    10921 accgaattgg cggtcagcgt cgatgtgcgt tccggcatag acattgccca agcgcgcgag
    10981 cagattgcct atctgcgcgg actcggacac agggtggaag ttttgtttgt cgaggcggaa
    11041 gaggccgtgc tggtccgccg cttttccgaa acccggcgcg gacatccttt gagcaatcag
    11101 gatatgacgc tgttggaaag cctgaaaaaa gagcgcgaat ggctgttccc gcttaaggaa
    11161 atcgcctact gtatcgacac ttccaagatg aatgcccaac agctccgcca tgcagtccgg
    11221 cagtggctga aggttgagcg taccggactg ctggtggttt tggagtcctt cgggttcaaa
    11281 tacggtgtgc cgaacaacgc cgattttatg ttcgatatgc gcagtctgcc caacccgtat
    11341 tacgatcccg agttgaggcc ttacaccggt atggacaagc ccgtttggga ttatttggac
    11401 ggacagccgt tggcgcagga aatggttgac ggcatcgaaa ggtttgttac gcgttggttg
    11461 ccgcgtttgg aggatgaaag caggagctac gttaccgtcg ccatcggctg taccggcggg
    11521 cagcatcgtt cggtctatat tgtcgaaaaa ctcgcccgaa ggttgaaagg gcgttatgaa
    11581 ttgctgatac ggcacaggca ggcgcaaaac ctgtcgggcc gctaatcccg tcaaaccatt
    11641 atgccgtctg aaaccccgtc cgccgtttca gacggcattt caaatattga gaagaaagaa
    11701 cagacatggc aatccaatgg tttcccggcc atatgaacaa ggcaagaaaa gccatcgccg
    11761 agcgtgccaa aagcgttgat atggtgattg aaatgctgga cgcgcgtatg cccgcctcca
    11821 gcgaaaatcc actgttggcg cagctttcca aaggtaagcc caaactcaaa attttaaaca
    11881 agcaagacct tgccgaccct gaacgcacaa aggtctggct tgaccactac aacagccgcc
    11941 ccgacacccg ggccatcgcc ctcgattcct ccgaaaccgg cgcacacggc aaaatcaccc
    12001 aagcctgccg cgcgatgatt ccccaccggc aaggcataga caaacccctg cgcgttttga
    12061 tttgcggcat tcccaacgtc ggcaaatcca ccctcatcaa tggaatgata ggcaaaaaat
    12121 ccgccaaaac cggcaacgaa cccggcatta ccaaagccga acaacgcctt ttccttgccg
    12181 acgacttctg gctgtacgac accccgggaa tgctgtggcc gaaaatcatc gtcgaagaaa
    12241 gcggctacaa cctcgccgcc ggcggcgcag tcggacgcaa cgcattggac gaagaagaag
    12301 tcgccctcga acttttagac tacctccgcc gccactacct ccctatgctg caagaacgct
    12361 accaagccga caaagacccc agcagccact gggacgacaa ctcgtggctc gaatggatag
    12421 ccaaaaaacg cggcgcagtc ctcagcggcg gacgggtcaa ctaccaaaaa gccgccgaaa
    12481 acatcctcac ggacttccgc gaaggcaaaa tcggcagaat cacccttgaa acaccgaacc
    12541 aatgggaaac ttggcttaaa aaagcacgtc agaaagaagc cgaactcaaa gccattcgcg
    12601 aagccagaaa agcagagcgc aaagggcaga agccttcgga agcataaaga atgccgtctg
    12661 aaaaatattt ttcaggcagc ttctctctac tccaaccgat ttcagacggc atatccaaac
    12721 ccatgccgtt tcagcacgga tacccgtatg accgacaaaa tttctcccga cgcgctgatt
    12781 gaagccgcac tgctgaccca aaccgaaccg ctgaccgaaa aatctatgcg cgaactgtgt
    12841 gtgccgccgt tgtcgcaaga caaactgatt gatgtgttgg cgcagttgaa aacgcgttgg
    12901 caggataggg cgttgcaact ggtgcatacg caggagggct ggcgttttca gattgttcag
    12961 acggcattcg agcggctggg cagcctgcaa gaacagcgtg cgccgcgcta ctcccgcgcc
    13021 gtgatggaaa cactggcgat tatcgcctac cagcagcccg taacgcgcgg cgacatcgaa
    13081 ggtatacgcg gcgtggcggt gtcgcagaac gtgatgcaga ccttgcagga tcgggggtgg
    13141 attgaagtca tcggacatcg ggacacattg ggaaaacccg cattgtgggc aacaacggca
    13201 acttttctca gcgatttgcg cttggacggt ttggaagaac tgccgccgct gaccgaactg
    13261 ggcgaactgg ttttgcccga tttgatagaa atgccgtcta cggatgaaga agagccggaa
    13321 accgtaccgt ccgataccct gcccaactga aattccaaat gccgtctgaa acgcac
//
LOCUS       DAXFWM010000066        13245 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000066 DAXFWM010000000
VERSION     DAXFWM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.066"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(94..1221)
                     /locus_tag="ACFI8B_001772"
     CDS             complement(94..1221)
                     /locus_tag="ACFI8B_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3834928.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKALKKISPDLVVPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDE
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEMIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            1252..1419
                     /locus_tag="ACFI8B_001773"
     CDS             1252..1419
                     /locus_tag="ACFI8B_001773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834929.1"
                     /translation="MRSALMKCRIRGAAGKPLPSAPHPTLRPPGQGRLQTAFYPEIRC
                     ITPSDIQYRVL"
     gene            complement(1448..1570)
                     /locus_tag="ACFI8B_001774"
     CDS             complement(1448..1570)
                     /locus_tag="ACFI8B_001774"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834930.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            complement(1575..2800)
                     /locus_tag="ACFI8B_001775"
                     /pseudo
     CDS             complement(1575..2800)
                     /locus_tag="ACFI8B_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3193..4440)
                     /gene="fabF"
                     /locus_tag="ACFI8B_001776"
     CDS             complement(3193..4440)
                     /gene="fabF"
                     /locus_tag="ACFI8B_001776"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC3834931.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(4596..4832)
                     /gene="acpP"
                     /locus_tag="ACFI8B_001777"
     CDS             complement(4596..4832)
                     /gene="acpP"
                     /locus_tag="ACFI8B_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC3834932.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5064..6071
                     /locus_tag="ACFI8B_001778"
     CDS             5064..6071
                     /locus_tag="ACFI8B_001778"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC3834933.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6268..6741
                     /locus_tag="ACFI8B_001779"
     CDS             6268..6741
                     /locus_tag="ACFI8B_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC3834934.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            6755..7090
                     /locus_tag="ACFI8B_001780"
     CDS             6755..7090
                     /locus_tag="ACFI8B_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834935.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNQHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            7284..9974
                     /gene="glnE"
                     /locus_tag="ACFI8B_001781"
     CDS             7284..9974
                     /gene="glnE"
                     /locus_tag="ACFI8B_001781"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC3834936.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPTGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFIKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            10398..12605
                     /gene="uvrD"
                     /locus_tag="ACFI8B_001782"
     CDS             10398..12605
                     /gene="uvrD"
                     /locus_tag="ACFI8B_001782"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC3834937.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRFGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            complement(13062..>13245)
                     /locus_tag="ACFI8B_001783"
     CDS             complement(13062..>13245)
                     /locus_tag="ACFI8B_001783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834938.1"
                     /translation="VLHHLREPFPLGRGGATPYRFLLIRYTCVMVSGTHHQPALHSVV
                     LSGNCPVVPMGCPAAP"
BASE COUNT         3157 a         3673 c         3581 g         2834 t
ORIGIN      
        1 cctggattcc cgctttcgcg ggaatgacgg ggtttcgcgg gaatggcggg agtttgtcag
       61 aaatcaccga aactcaaaag ccgaccacct tgtttacgcc ttcaaaatat cgagaaattt
      121 caaatcgact ttttcggcat cgaatttatc tttggcaatc gcataacttg cattccccat
      181 caggcggacg gcagccctgt tttcgataaa ataaatcatt ttttcggcca agatgcgggg
      241 attccaaggt tcgatcagga agccgttgac cttgtcggca accgtttccc tgcatccggg
      301 gacatccgtc gtaatcaccg ccctgccgac ggccattgcc tcctgagtgc tccggggaac
      361 gccttcccta taataagacg gcaatacgaa tatatgatgt tcttttatca tttcggaaac
      421 attgttcaca aaaccgggga aacggataat atcgcgggcg gcaggctgtt ccaaatcccc
      481 gctcccgcgt ggtttgtcga ttgcgcccaa agcggtaaaa accgtatcgg ggtatttgcc
      541 cttaacctgt tccgccgccc gaataaactc atcaatccct ttttctttca gaaatctgcc
      601 gataaagagg aattttacgg gttctttttc atcgggaata tccgcctcgg aataaggata
      661 ttgccgcaaa tccagaccga ttccgcccaa aatatggatg ttttttattt tgatgccgta
      721 ttgatgcagc aactcgtctt tgtcgtcggg gtttaataca atcaggcttt ccaacatcgg
      781 cagggtaatg cggtacaagg cgattaaaat cccctttatg atttttgttt ttaacggtat
      841 gccttccggc tgcggggtaa atgcgaatcc caaaccttcc agcatcccga cgattctggg
      901 cacgcctgcc aattttgcgg caaaagtgcc gaaaatcacg ggttttgcga aataagggac
      961 aaccaaatcc ggcgatattt ttttgagtgc tttaaatatg aggaaggtgg attttatatc
     1021 ggaaaacggg tttagcccgc tgcggtttga ccggtaggta acgggtgtaa cccccatttc
     1081 cctgataatg tccaactcat tatcggaaaa ctccgataca aaggcataca cctgatggtt
     1141 tttgccgatt aattttttaa tgacgggggc gcggaaaccg taaatgctgg atgcgactgt
     1201 tgtgataaaa acgattttca taaggcggac accttgaata taggattgga aatgcggtct
     1261 gcattgatga aatgccggat acggggcgcg gcgggcaaac ctttgccaag cgcgccgcat
     1321 ccgacgcttc gcccgcccgg gcaggggcgg cttcagacgg cattttatcc tgaaatccgc
     1381 tgcataacgc ccagcgatat acagtatcgc gttttgtgat gattttacac ccgaagggaa
     1441 aacgggctta tttgaatggc ggtttaatcg cggcggttat ttgcggtttg ataaggctga
     1501 tttgcgtgcc gtgcagtccg gcaacgggcg gggtgtttgg aaaatgccgt ctgaaggttt
     1561 cagacggcat tctgtcatgc ggcggttcgg ggttcgcgga cgggcaggac aagcagggct
     1621 gccgtgccgg cgagtacggc atcggcgtac cacatccagc cgtagtcacc gaattgtgta
     1681 atcacgatgc cgccgatgta cgagccgagg aatccgccga tttggtgggt gagcatcacc
     1741 agtccgaaca gggtggcgag gtagcgcgtg ccgaagagtt tgccggtaac ggcggcggtc
     1801 ggggcgacgg tggcgagcca tgtgaatccg agtgcggcgg cgaaaatgta aaagttgagg
     1861 tcggttttgg gtgagaagat gtaaatcagc accatggcga cgcgtgaggc gtagagtccg
     1921 aacaggatgt gttttccttt gaagcgtccg gtgcatcagc ctgagaacac gcaaccggcg
     1981 atgtttgcca gtccgatgat ggcgatggat gtcgaggcga cggtggcggg cagtccgcac
     2041 agggcgactt ccgtgggtag gtgggttacg agaaaggcga tgtggaagcc gcaggcgaaa
     2101 aagctcaggt gcagcaggat gtagcttggg gttttgaagg cggttttgac tgcttctccg
     2161 aggctttgtc cgtgtgtggc ttgggtgtgt tgggtgtggg cggcgttgtt gccgccggca
     2221 agccaccatg agacgggcag aatcagcagg gcgattgcgc cccaaacgta aaatgtaccc
     2281 gtccagccga cttcgggcag gacgacgagt ccttgagtcc ttgaaccagc ggtgcgaaca
     2341 ggaattgtcc tgccgaaccg cctgcgttga ccagtccgga tgccaagccg cgtttgtgtg
     2401 tggggacttg ggcggcgacc tgtcccataa tgatggaaaa cccgccggaa ccggtgccga
     2461 atgcgagcag cagcccgacg gcaatcatca gcccccaata agtcgggatg ttggaggcaa
     2521 tcaggcaggc gcagacgagc aggagggcac cgccgcttaa taccctgaac gcgccgaaac
     2581 ggtcggcaag cgcgccggac aatggttgca atacgcccca catcagttgg aaaacggcga
     2641 tgatgaggct gaactgggca atgttcaatt cggtggtgtt gacgacgggt tggacgaaca
     2701 gtccgagcgt catcctcatg ccgatggtaa tcagcaggat gaatgcggcg gcggcaatga
     2761 ccgcccagag tttgggtgtt ttcgatgcgg tgtgcgtcat aatatgtatt ttgagtcggt
     2821 taagtttgcg aattttaaca aaccggatga ttcgggctaa ataattcctg attctttgga
     2881 tatagccaac ggtatgtctg ccgatgccgt ctgaaagggc ttgatgggcg tttgcggttc
     2941 gggttaatat ttgtgccgta tttgttaatt ccagttagta tagtggatta actttaaacc
     3001 ggtacggcgt tgccccgcct tagctcaaag agaacgattc cctaaggtgc tgaagcaccg
     3061 ggcgaaccgg ttccgtacca tttgtaccgt ctgcggcccg ccgccttgtc ctgatttttg
     3121 ttaatccact ataaaaatgc cgtctgaaac ggtttcagac ggcatttcga tgtcggcggc
     3181 ggctttgcgg aatcagcctt tgaagcgttt gaagaccagc gtaccgttgg tgccgccgaa
     3241 gccgaaggag ttggaaatgg caacgtcgat ttccgcgtcg cgcgcttcgt tggcgcagta
     3301 gtccaaatcg cagccggctt caacgtcttg ttcaaaaatg ttgatggtcg gcgggatttt
     3361 gccgtcgtgt atcgccaaaa tgctgtacac ggcctccacg ccgcccgccg cgccgagcag
     3421 gtggccggtc atggatttgg tcgagctgat gacggttttg caggcgtgtt cgccgaacgc
     3481 gcgtttgagg gctttggttt cgttggcatc gcccaagggg gtggacgtgc cgtgcgcgtt
     3541 gacgtaatcc acgtcttcgg gattgatgcc ggcatctttc agcgcgcggg taacggcaag
     3601 ggcggggcct tcttcgttcg gcgcggtgat atggtaagca tcggaactca tgccgaagcc
     3661 gacgatttcg gcgtagattt tcgcgccgcg ttttttggcg tgttctaatt cttccaacac
     3721 caatatgccc gcgccttcgc cgatgacgaa gccgtcgcgg cctttgtccc acggacggga
     3781 agcggtggcg gggtcgtcgt tgcgggtgga gagggctttc atcgcagcaa aaccgcccac
     3841 gcccaaggtg ctgattgcgc cttccgcgcc gccggcaacc attatgtccg cgtcgccgta
     3901 tttgatcagt cgggcggaat cgccgatggc gtgcgcgccg gtggtacagg cggaaaccat
     3961 gccgtagctc gggccgcggt agcctttgag gatggtaacg tgtccggaaa tcaggttgat
     4021 cagcgaaccg gggataaaga aagggttgat tttgcgcgcg ccgccttcga ttacggcttt
     4081 gccggtggcc tcgatgctgg gcagtccgcc gatgccggaa ccgatgttca cgccgatgcg
     4141 gtctttgtcg aggttttcca catcgtccaa acccgaatcg gcaatcgcct gcaatgcggc
     4201 ggcgatgccg tagtggatga acacgtccat ccggcgcgct tctttcgcgc tgatgtattg
     4261 tccgatgtcg aaaccgcgca cttcgccggc gacacggctg ttgatgtcgg atgcgtcaaa
     4321 gcgggtaatc gcgccgatgc cgcttttgcc ggcgagcagg ttgtcccaag cctctgcggc
     4381 agtgttgccg acaggggaaa cctgacctaa gcctgtaatg actactcttc tctgactcat
     4441 gataacctcg ctgttggttg tcggaatggg ggcatatgcg gctgtcgtgc agatgccgtc
     4501 tgtaatttgc ggcaggggtt caaacagttt gccatataag ggaaaagcct ctattgcgcg
     4561 gtgcagcaga ggctgttgtg tcgggcgacg accggttagc cgttgtgggc attgatgtag
     4621 tcgatagcca gttggacggt ggtgattttt tcggcatctt cgtcggggat ttcgcagccg
     4681 aaggcttctt ccaaagccat aaccagctcc acggtatcca aagaatccgc gcccaagtcg
     4741 tcttggaagg aagattcgtt tttcacgtcg gcttcgttta cgcccagttg ttcagcaata
     4801 attttcttaa cttgttgttc gatgtttgac atatcagtcg ttcctttatg ccttgcggca
     4861 ggttgtttaa gggaaataaa tcggtggtat tgtaccgatt tttaatagag ttttctatct
     4921 aatgcctatt atatcaatat ttgcggattt gtacattttt gggtgcggcg ggttttgtcg
     4981 ttcaagtttg acctgtgtgc cgtatgtttg gcgggatttc ggttaaaatg gcggcatttc
     5041 catctgaagc agaaagccct gtcatgtatc cacttgcccg ccgcatcctg tttgcactcg
     5101 atgccgaaaa agcccaccac ttcacgctcg acgcgctcaa cacggtttat aaattgggcc
     5161 tgattcccgt aaccgacaac cgtaccaaac ctataaaact gatgggtatg gatttgccca
     5221 atcctgtcgg gcttgccgca ggtctcgaca aaaacggtga atacatcgac gctttgggcg
     5281 cgctcggttt cggcttcctc gaaatcggca cggttacgcg caatccgcag cccggcaacc
     5341 cgcagccgcg cctcttccgc gttcccgaac accaaggcat catcaaccga atgggtttta
     5401 acaaccacgg catcgacgcg atgatacgca atattgaaaa aagcaaatat caaggcgtat
     5461 tgggcatcaa catcggcaaa aacgcggtta cgcccatcca aaacgccgcc gatgattatt
     5521 taatctgcct cgaaaaagcc tacgcgcacg caagctacat taccgtcaac atttcctcgc
     5581 ccaacaccaa aaacctccgc gcgctgcaag gcggcggcga attgggcgca ttgctcgaag
     5641 ccctgaaaaa caaacaggcg cagcttgccg ccgcccacgg caaatacatc ccgctagccg
     5701 tcaaaatcgc ccccgatttg gatgaagcac aaatcgaaga catcgcccgc gtcgtcaaat
     5761 ccgtcgaaat ggacggcatc atcgctacca acaccaccat cgacaaatca agcctcggca
     5821 gccatccgct cgcaggcgag cagggcggtt tgagcggttt tcccgtgcgc gaaaaaagca
     5881 accgggtgct gaaaaaactg gcggaacaca tagacggcgc attgccgatt atcggcgtag
     5941 gcggcattat ggaaggcggg gacgcggcag agaaaatccg cttgggcacg accgccgtcc
     6001 aagtgtacag cggattgata tacaaaggcc cggcgttggt caaagaatgt ttgaaggcat
     6061 tggcgcgatg acgcgcccca cccaaaatgc cgtctgaacg cacgttttgc cgttcagacg
     6121 gcattttcat agattggtat ccgactgttg aagaggcaat cagccgtcat ttggaagact
     6181 tttcgtaaca ggcgtcaatc gaaaggggcg gaatgacggg gcaggcaaac cgatgcgtga
     6241 acacggggca ggcaaccgga gtaaaaaatg aaccttgatt taaccgcgca aaaagtccgc
     6301 ctttcttgga aggatattct gtgggggtat gagaataaat acttgggttg ggctgatgtg
     6361 gcagcttatg cccgaaaaat gacgctttca gatcatgatg aacgcgtgtt caagctatct
     6421 ttaaccaaca aatccaatat ttttgaatta aagcctgttc tggaagattt ggcttcggaa
     6481 acgaggggtt attcccctaa aaattggctg tacatcctcc taaatgacgt attccataga
     6541 aaagaagaat ttgaggatcc tctgggggaa gttgaaaaaa tttatgcgga ttttgattac
     6601 ccggaagaaa tagaatcatt tgtcaggtat atgccgccca aagacggcta tatcccttct
     6661 aatcatagtt atgaagaaaa tattgcccgg ttatattctc actgggaaca ctatttgaac
     6721 aacggcggcg ggcagggtta aaaccggcaa tccgatgccg tctgaagcgt tatccggcgt
     6781 tcagacggca ttttgttttc cgacagttta taaactgtcg ttgtttcttg acagaaacaa
     6841 cgaccttatt tatttgaaac gattggagga catgattatg ggtttttggg gtagtgtggg
     6901 aaaagcagca aaagcagtag gcgagggatt gattgaagcc ggcaatcagc ataaggcgtt
     6961 gaaaatggaa tatgcggaga aatcaagtga ggagctgcat gaaatcgtaa agagtgatgg
     7021 tttttttaaa aattccacac gggagaaagg tgcggcttat gctgttttaa aagagcgtgg
     7081 cgaggtttga acgggaaacg gcggcatttg ccgctgtttt tttatcggca ggcatccgtc
     7141 cgaatatcgg ggcaaggttt cagacggcat cgaaggttgc tatgatatag tggcttgact
     7201 ttaaaccggt acggctccgc cttgccctga tttgaagttc atccgctatc acattcccgc
     7261 catcctttca aacggaatcc gaaatgtccg accaccgcct cgacaccgcc cgccgccatt
     7321 ccctgttcct cgcccgccag ctcgacaacg gcaaactcaa gcccgaaatt ttcctgccca
     7381 tgttggacaa ggcgttgacg gatgaggatt tccaagcctt tgccgactgg gacaaaatcc
     7441 gcgcggaaga aagcgaggaa gaattggcgc ggcagttgcg cgagttgcgc cgttatgtgg
     7501 tgtcgcagat tatcgtgcgc gatataaacc gcatcagcga tttgaacgaa gttacccgca
     7561 cgattacgct gtttgccgat tttgccgtca ataccgcgct ggatttcgcc tacgcctatt
     7621 atcgggatat gtacggcacg ccgaccgggc gttataccaa atcgccgcag catttgagcg
     7681 tggtggcgat gggcaaggcg ggcggctatg agctgaacgt gtcttccgac atcgatttga
     7741 ttttcgtcta tcccgaatcg ggcgacaccg acggcaggcg cgaacggggg aatcaggagt
     7801 tttttatcaa agtcgggcag aaactgattg cattgttgaa cggcattact gccgacgggc
     7861 aggtgttccg cgtcgatatg cggctgcggc cggacggtga ttcgggcgcg ctggtgttga
     7921 gcgaaaccgc gctggagcaa tatctgatta cgcaggggcg cgaatgggaa cgctatgcgt
     7981 ggtgcaaagg ccgcgtagtc acgccgtatc cgaacgacat caaatcgctg gtgcgcccct
     8041 tcgtgttccg caaatatctg gattacggcg cgtatgaggc gatgcgcaac ctgcaccgcc
     8101 aaatccgcag cgaagtcagc aaaaaaggca tggcggacaa catcaaactc ggcgcgggcg
     8161 gcatccgcga ggttgaattt atcgcccaga ttttccagat gatacgcggc ggccaaatgc
     8221 gcgcgctgca actgaaaggc acgcaggaaa cgctgaagaa aattgccgag atgggcatta
     8281 tgccgtctga aaacgtcgaa accctgcttg ccgcctaccg cttcctgcgc gatgtcgaac
     8341 accgcctgca atactgggac gaccggcaaa cccaaaccct gccgatctcg cccgaacagc
     8401 ggcaactgct cgccgaaagc atgggtttcg acagttatgc cgctttttca gacggcctca
     8461 atgttcatcg gaacaaagtc aatcagttgt tcaacgaaat tttgagcgaa cctgaagaac
     8521 aagcgcaaag caatagcgaa tggcaatggg catggcagga aaaacccgac gaagaagaac
     8581 ggctaggccg tctgaaagaa cacgggttcg atgccgaagc cgtcaccgca aggctcgaac
     8641 aaatctgcca cggacataaa taccgccgcc tttccgcaca cgcccagccg cgctttgaca
     8701 ccattgtgcc gctgttcgta caggcggcgg cagagcaaaa caacccgaca gatacgctga
     8761 tgcggctgtt ggattttctc gaaaacatca gccgccgctc cgcctatctc gccttcctca
     8821 acgaacatcc gcaaaccttg gcacaactgg cgcagattat gagccaaagc tcgtgggtgg
     8881 cggcgtatct gaacaaatat ccgattttgt tggacgaact catcagcgcg cagcttttgg
     8941 ataccgcgtt tgattggcag gcactcgccg ccgccctttc agacggcatc gaagcctgcg
     9001 gcggcgatac cgaagcgcaa atggacacgc tgcgccattt ccaacacgcc caagtcttcc
     9061 gcctcgccgt ccaagacctc gccggattgt ggacggtaga atccctctct gaccaactct
     9121 ccgccctcgc cgacaccgtc attgccgccg ccctttcgtg cgcgtgggcg gatatgccca
     9181 aaaaacaccg cgacaccccg caattcgccg tcatcggcta cggcaagctc ggcggcaaag
     9241 aactcggcta cgcctccgac ctcgacttag tctatcttta cgacgatccc catcccgaag
     9301 caggcgacgt gtacagccgc ctcgcccgcc gcctgaccaa ccggctttcc gccgccaccg
     9361 gcgcgggcag cctctacgaa accgacctgc gcctgcgccc taatggcgac gccggtttcc
     9421 tcgcgcacag catcgccgcc tttggaaaat accagcgtga aaacgcatgg acatgggaac
     9481 accagtccct cacccgcgcc cgcttcatct gcggcacacc cgaaattcag acggcattcg
     9541 accgcatccg caccgaaatg ctgactgccg aacgcgacca aaccgccttg gcaggcgaga
     9601 tcatcgaaat gcgcgaaaaa atgttcccca cccatccgcc tgccgacagc aacgtcaaat
     9661 acgcgcgcgg cggcgtggtc gacgtcgaat ttatcgtcca atatctgata cttgcccatg
     9721 cccgccagta tccgcaactc ttggacaact acggcaacat cgccctattg aacatcgccg
     9781 ccgactgcgg tttgattgac aaaaccctcg ccggacaaag ccgcaccgcc taccgcctct
     9841 accgccggca gcagcacaac accaaactgc gcgacgcggc aaaaaccgaa gtaagcgacg
     9901 aattgctgtc ccattacggc aatgtcagga aattgtggcg ggaagtgttc ggggaggaag
     9961 cggcaaccgc ctgaacaaaa aatgccgtct gaagcctgac aatccgggtt tcagacggca
    10021 tttgtttttg gctgtgtctg aacaatcggt acgatttcgg ttgtttgcaa attgaaaggc
    10081 ggttattcct atgttcaggc ggcaaaccga aatattttcc ccaaccgata gggcgatatc
    10141 aaagcctatt cgaataccgc ctttacgggg aaggggcttt ccgagcttga ccatggtcag
    10201 gctcggcaaa tcagcccgca tcctgtcgat gcgtccgtcc aaatcggttt ggaacgccgc
    10261 aatgtgatat ccgcctatca aggttttgcg tgcgtgtgtc ggcgagggct ttgccaaaac
    10321 cgaaatcaaa atcaagttgt caggcatcct tccgtttcta ttaaaatagc gcattccatt
    10381 tttcagacgg catccttatg tttcccgacc aatccgcccc caatctgctg caaggcttga
    10441 accccgaaca actctccgcc gtaacctggc cgccgcaatc cgccctcgtt ttggcaggcg
    10501 caggcagcgg caaaacgcgc gtgctgacca cgcgtatcgc atggcttttg caaagcggac
    10561 aagccagcgt acacagcatt atggcggtaa cgtttaccaa caaggccgcc aaagagatgc
    10621 aaacccgatt cggcgcaatg attcccatca acgtccgcgc tatgtggctc ggcacgttcc
    10681 acggtttgtg ccaccgcttt ttgcgcctgc accaccgcga cgcaggcctg ccgtcttcct
    10741 ttcaaatcct cgacagcggc gaccagcttt ccctcatcaa acgcctgctc aaaagcctca
    10801 acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg ctttatcaac gcgcaaaaag
    10861 aatccggttt gcgcgcttcc gtcttgggcg cgcccgatcc gcacacaagc cgcatgattg
    10921 ggtgctacgc cgaatacgac aaaatctgcc aacgcgaagg cgtggtcgat ttcgccgaac
    10981 tcatgctccg cagctacgaa atgctgcaaa gtaacgaaat cctgcgccgg cactaccaaa
    11041 accgcttcaa ccacattctg gtcgacgagt ttcaagacac caacaaactg caatacgcct
    11101 ggctcaaact catggcgggc ggtaacgcag cggtatttgc cgtcggcgac gacgaccaaa
    11161 gcatttaccg cttccgcggc gcaaacgtcg gcaacatgac cgcactgatg gaagaattcc
    11221 acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc cgtcggcaac atcctcgccg
    11281 ccgccaacgc cgttatcgaa aacaacgacg aacgtctcgg caaaaacctg cgcaccgacg
    11341 ccgaagcagg cgacaaaatc cgctactact ccgcctttac cgacctcgaa gaagcccggt
    11401 tcatcgtgga tgaaaccaaa gccctcgaac gcgaaggctg ggatttggac gaaatcgccg
    11461 tcctctaccg cagcaacgcc caatcgcgcg tcatcgaaca aagcctgttc cgcagcggca
    11521 tcccctacaa aatctacggc ggcctgcgct tttacgaacg ccaagaaatc aaacacgccc
    11581 tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa cgccctcttg cgtgtcatca
    11641 acttcccacc gcgcggcatc ggcgcgcgca ccgtcgaaaa ccttcagacg gcctcaaacg
    11701 aacaaggcat caccctctgg caggcagcct gcaacgccgg cgcgaaagcc gccaaagtcg
    11761 ccgccttcgt ccgcctgatt gaagccctgc gcaaccaagt cggacaaatg cacctgtccg
    11821 aaatcatcgt cggcatcctc aaagacagcg gtctgaccga gcactaccga acccaaaaag
    11881 gcgacaacca agaccgcctc gacaaccttg acgaactcgt caacgccgcc atcgagttca
    11941 aacccgaaga cagcaacttt gaaaccctgc ccgaaaacat ttcagacgac cccgccttcc
    12001 ccatcctcgc cttcttgagc aacgccgccc tcgaatccgg cgaaaaccaa gcaggggcag
    12061 gcgaaaaagc cgtccaactg atgaccgtcc acgccgccaa aggcttggaa ttcaacgccg
    12121 tcttcctcac cggtatggaa gaggggcgtt tccccagcga aatgagcctt gccgagcgcg
    12181 gcggcctcga agaagaacgc cgcctcatgt acgtcgccat cacccgcgcc cgcaaacgcc
    12241 tctacatcac catggcacag caacgcatgc tgcacggaca aacccaattc ggcatcgcat
    12301 cccgcttcgt cgaagaaatc ccgccagaag tattgcacta cctgtccgtc aaaaaacccg
    12361 ccttcgacag ctacggcaac acgcgccaaa ccaccgtaca acgggacaaa atcatcgatg
    12421 acttcaaaca gccgcaaacc tacgcaggct tccgcatcgg acaaaacgtc cgtcacgcca
    12481 aattcggcac cggcgtgatt atcgatgccg cagacaaagg cgaatccgcc cgactgacca
    12541 tcaacttcgg caaacagggc gtgaaagagc tggataccaa gtttgcgaaa ttggaagcga
    12601 tgtaaatttg aaatgcaggc cggatattcg tatccgacaa aaaaacattt gacgcgttta
    12661 tcgtttccga aaaaccgctg ttggaaatgt cggattcgag aatccgacct acggcaaaaa
    12721 accgtagcaa ggacaaggca aatagtcgtc tgaaaacggg aagggcaatt ttgccgcagc
    12781 cgccgccgtc attcccgcga acctacattc cgtcattccc acgaaagtgg gaatccggaa
    12841 cgcaaaatct aaagaaaccg ttttatccga taagtttccg caccgacagg tctggattcc
    12901 cgcttatgcg ggaatgacgg cggcgggaat cggcggattc ggcggagttg gtggatttgg
    12961 cgggctgaag cccgcccttg tatatcggaa cttccgtatc atagcgacaa accgcccggc
    13021 cgccacccgc gcccgcccaa ggcagacaac cgttgcgtag ctcagggagc ggcagggcaa
    13081 cccatcggca caaccggaca gttgccggac aacacaaccg aatgcaaggc aggttgatga
    13141 tgagtacccg ataccattac gcaggtatag cggattaaca aaaaccggta cggcgttgcc
    13201 ccgccccggc ccaaagggaa cggttcccta aggtgatgaa gcacc
//
LOCUS       DAXFWM010000067        13221 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000067 DAXFWM010000000
VERSION     DAXFWM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.067"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(256..741)
                     /gene="purE"
                     /locus_tag="ACFI8B_001784"
     CDS             complement(256..741)
                     /gene="purE"
                     /locus_tag="ACFI8B_001784"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC3834939.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(789..1502)
                     /locus_tag="ACFI8B_001785"
     CDS             complement(789..1502)
                     /locus_tag="ACFI8B_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC3834940.1"
                     /translation="MKTKLPLFIIWLSVSASCASVSPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1502..2170)
                     /locus_tag="ACFI8B_001786"
     CDS             complement(1502..2170)
                     /locus_tag="ACFI8B_001786"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3834941.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2181..2366)
                     /locus_tag="ACFI8B_001787"
     CDS             complement(2181..2366)
                     /locus_tag="ACFI8B_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC3834942.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2510..4486
                     /gene="mutL"
                     /locus_tag="ACFI8B_001788"
     CDS             2510..4486
                     /gene="mutL"
                     /locus_tag="ACFI8B_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC3834943.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4581..6704
                     /gene="dnaX"
                     /locus_tag="ACFI8B_001789"
     CDS             4581..6704
                     /gene="dnaX"
                     /locus_tag="ACFI8B_001789"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC3834944.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SVPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5770..5809
                     /gene="dnaX"
                     /locus_tag="ACFI8B_001789"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6785..7120
                     /locus_tag="ACFI8B_001790"
     CDS             6785..7120
                     /locus_tag="ACFI8B_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC3834945.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7773..8819)
                     /gene="recA"
                     /locus_tag="ACFI8B_001791"
     CDS             complement(7773..8819)
                     /gene="recA"
                     /locus_tag="ACFI8B_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC3834946.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            9019..9783
                     /gene="aroD"
                     /locus_tag="ACFI8B_001792"
     CDS             9019..9783
                     /gene="aroD"
                     /locus_tag="ACFI8B_001792"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC3834947.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9803..11818
                     /gene="rep"
                     /locus_tag="ACFI8B_001793"
     CDS             9803..11818
                     /gene="rep"
                     /locus_tag="ACFI8B_001793"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC3834948.1"
                     /translation="MMKLNAQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11888..12946)
                     /gene="dinB"
                     /locus_tag="ACFI8B_001794"
     CDS             complement(11888..12946)
                     /gene="dinB"
                     /locus_tag="ACFI8B_001794"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC3834949.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
BASE COUNT         3224 a         3841 c         3430 g         2726 t
ORIGIN      
        1 cccgatttga cgatgcacta ctgtcctatt ttgattttaa ttgactatat ttgtactgtc
       61 tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tatatatttc aaaaattgaa
      121 acccgcacag attgttaaac cccaaaacag gggcgggcga aattcttccg gcaatgggtt
      181 catgcaaaca aaatgccatc tgaaccccga aaacagggct tcagacggca tttgtgtatt
      241 cgcggacggc gggttttaaa tctgttccaa ttccatgttt aaaacggttt gttcctgttt
      301 ggctcggaag tctgccagtt tttgcgccag ttcggggttt tcgttggcga gcatggagat
      361 ggcgaacaat gcggcatttg ccgcgcctgc ctcgccgatg gcgaatgtgg cgacgggtac
      421 gcctttgggc atttgtacaa tcgataaaag cgaatcttcg ccgcgcaggt atttgcttgg
      481 gacgggtacg cccaaaacgg ggacggtggt cttggcggca accatgccgg gtaaatgcgc
      541 cgcgccgccc gcacccgcga tgatggcttt gatgccgcgc gcccgtgcgg tttcggcgta
      601 ttcaaacatc aaatccggag tgcggtgtgc ggaaacgacg cgcgcctcat attctacgcc
      661 gaactcttca agaaactgcg ctgcctgccg cataacgggc caatcgctgt tgctgcccat
      721 gatgatgccg atttgtatca taaatccttc ttggtgcgga tggggtaaaa agcggaaaaa
      781 tggaaaaact atcgtttgcg tacggctgcg gcggcgcgtt ttgccgccgg gctgccggga
      841 taggtctgta tcaggctgcg ccaagtcgcc cttgcaatgt ctttttgctg aagcctgtat
      901 tggcattcgc cgattttgaa tatgacttcg ggcgcggttg ggctgtcttt gaaacggttg
      961 gcgtaacgcc ctccgatttc gatgacagat tcacagttcc ccatacgcgc cctgctttgc
     1021 agcaacaggt acatactgcg ttgcgcgatg ctgccgccgt ctccgccgtc cgcccccttc
     1081 aacaaggcgg ctgcggcaga aaacctgccg ttttgatagt gtttgagtgc ctgattgtag
     1141 aggttttgtg cggtttcgac ggtatgtgcg gatgcgctgc cgccttcggt attgaggtaa
     1201 tgctctttca atttgcggtc gtcgagtttt tggacgtatg tcctgccgga agggtgtgtt
     1261 tttgcgtgtt ccaatgcttt gactttcccg tttaacattt ccacttcgtt cgacagccgg
     1321 acgattttgc cttccagata gtccaaacgg tcttgcagag tgggaacggg atagggaatg
     1381 ccgtctgaag cattttcctg tgtcggcatt tcggttcggc tgccctccgg aacgggtgaa
     1441 acggaagcac aggaggcaga cacagacagc caaatgataa aaagcggtaa tttggtcttc
     1501 attatttttt cagaagcagg gtcaaaccgt cgccgacggg cagggtgatg gggacgatgc
     1561 gcgtatcgtt cggcaggttt tgattgaaat ctttgagaat tttgacactg ggcggcgcat
     1621 caaaagccgc ttcgcgcatc acccttccgt tcaacaaaat attgtcgatg gcgatgatgc
     1681 cgccttgccg gacgagtttg aggcaacgct cgaaatattg cggcgtgggc ggtttgtctg
     1741 cgtctatcag tgccaaatcg tagcttccgg cttcaccctg tgcaatcaaa tcatccaatg
     1801 tcagcaatgc gggttgcagg tgcaggctga ttttatgcgc cacaccggcc tcgttccaaa
     1861 cctgacgcgc cgtatcggta aaggttacat tgatgtcgca ggcggtaatc cgcccgtgtt
     1921 cgggcagcgc caatgcaagc gcggtgctgc tgtatccggt aaatacgccg atttccagat
     1981 atttttccgc gcgaatcagc tttgccagcc aaaccaaaac cgccgcctgt tcgcgcgcaa
     2041 tcgccatttt gcccatacgg tgatgcccgg ccttctcacg cagccgcgtc aaaacgggat
     2101 gttcgggttc gccgatggca ttcaaatagt tttgcaggtc cggtgcgaca ttggacagat
     2161 gggtcgtcat ttcggcgggt tcagtcttgg taataggtat aaggtttttt cgccactttt
     2221 gccgattcaa agtttttttg ctcttcggga ttgagttcga catcccacaa aagccctcga
     2281 tttttcaaac gttcttgcgc caattcaggt ttttcttcga tcaggcggtt gaggaattgt
     2341 gtggcttcgg attggtagtg gtacatcttt gcgctctaat tttacgggat acggcgtgat
     2401 tatactggta ttttccaaac gggataaacg gcttttatca agaatacggg cagaaagata
     2461 aggggtttta ttatagaata aggcgttttt tgcaacggaa gaccgcctta tgccacgcat
     2521 tgccgccctg cccgaccacc tcgtcaacca aattgccgcc ggcgaagtgg tcgaacgccc
     2581 tgccaacgcc ctgaaagaaa tcgttgaaaa cagcatagac gcgggcgcaa cggcggttga
     2641 tgtggaactg gaaggcggcg gcatccgcct catccgcgtc ggcgacaacg gcggcggcat
     2701 ccaccccgac gacatcgaac tcgcgctcca ccgccacgcc accagcaaaa tcaaaaccct
     2761 gaacgatttg gaacacgttg ccagcatggg ctttcgcggc gaaggcttgg ccagtatcgc
     2821 ctccgtcagc cgcctgaccc tgaccagccg ccaagaagac agttcgcatg cgacccaagt
     2881 caaagccgaa gacggcaaac tcagcagccc caccgccgcc gcccaccccg tcggcaccac
     2941 catcgaagcc gccgaactct tcttcaacac ccccgcgcgg cgcaagttcc tcaaatccga
     3001 aaacaccgaa tacgctcact gcgccaccat gctcgaacgc ctcgcgctgg cgcatccgca
     3061 cattgccttc tcactcaaac gcgacggcaa acaagtgttc aaacttcctg cccaaagcct
     3121 gcatgaacgt atcgccgcca ttgtcggcga tgattttcag acggcatctt tggaaattga
     3181 cagcggcaat agcgcgctgc ggctctatgg cgcgattgcc aagccgactt tcgccaaagg
     3241 caaaaccgat aaacaatatt gcttcgtcaa ccatcgtttt gtccgcgaca aagtcatgct
     3301 ccatgccgtc aagcaggcat accgcgacgt attgcacaac gccctcaccc ctgctttcgt
     3361 ccttttcctc gagctgccgc ccaaagccgt ggatgtcaac gtccacccga ccaaaaccga
     3421 aatccgcttc cgcgacagcc ggcaagtaca ccaactcgtg ttccacacgc tcaacaaagc
     3481 ccttgccgac acacgcgcca acctgaccga aagcgtcagc aacgcaggcg aagtgttgca
     3541 tgacattacc ggcgttacgc ctgccccaat gccgtctgaa aacgacagcg aaaatctgtt
     3601 tgatagcgca tccaaccacc cgacaggcaa caaacccgat acacgcaatg cctttggttc
     3661 atcaggtaaa accgcgccca tgccttacca ggccgcgcgt gcgccgcaac aacacagcct
     3721 gtccctgcgc gaaagccgcg cggcaatgga cacctatgcc gaactctaca aaaaaaccga
     3781 cgacatcgac cttgagttga gccaattcga acaggcacgt ttcggcaata tgccgtctga
     3841 aacgcctgct cacaaaacag atacgccgct ttcagacggc atcccgtccc aatccgaact
     3901 gccgccgctc ggtttcgcca ttgcccaatt acttggcatc tacattcttg cccaagccga
     3961 agacagcctg ttgctcatcg atatgcacgc cgctgccgaa cgcgtcaact acgagaaaat
     4021 gaaacgccaa cgtcaggaaa acggcaacct gcaaagccag cacctgctta ttcccgtaac
     4081 ctttgccgcg tcccacgaag aatgcgccgc cctcgccgat catgccgaaa cgctggcagg
     4141 cttcggcctg gaactgtccg acatgggcgg caacaccctc gccgtccgcg ccgcgcccgt
     4201 aatgttgggc aaatccgatg tcgtctccct cgcacgcgac gtattgggcg aactcgccca
     4261 agtcggcagc agccaaacca tcgcatcaca cgaaaaccgc atcctcgcca caatgtcctg
     4321 ccacggctca atccgcgccg gccgcaggct caccctgccc gaaatgaacg cgctgctgcg
     4381 cgatatggaa aatacgccgc gcagcaacca gtgcaaccac ggcaggccga cgtgggtcaa
     4441 actgactttg aaagaattgg acacactgtt cttgcgcgga cagtaagccg aaaatgctag
     4501 aatacgccgc ccgaagccgc cgttcagacg gcattccgac gcaccgacag aaacatcacg
     4561 accgaaacca agagaaaaac atggcctatc aagttctcgc ccgaaaatgg cggcccaaaa
     4621 ccttttccga cttagtcggt caggaacacg tcgtcaaagc cctgcaaaat gccttggacg
     4681 aaggcaggct gcaccacgcc tacctgctga ccgggacgcg cggcgtgggc aaaaccacca
     4741 tcgcccgcat ccttgccaaa agcctcaact gcgaaaatgc gcaacacggc gaaccttgcg
     4801 gcgtgtgcca aagctgcacc caaatcgatg ccggacgcta tgtcgacctg ctcgaaatcg
     4861 acgccgcctc caacacaggc atcgacaaca tccgcgaagt cttggaaaac gcccaatacg
     4921 cgccgaccgc cggcaaatac aaagtctata tcatcgacga agtgcatatg ctttccaaaa
     4981 gcgcgttcaa cgccatgctc aaaacgctgg aagagccgcc cgaacacgtc aaattcatcc
     5041 tcgccaccac cgatccgcac aaagtccccg ttaccgtatt aagccgctgc ctgcaattcg
     5101 tcttacgcaa tatgaccgcg caacaggttg ccgaccacct tgcccacgtc ctcgacagcg
     5161 aaaaaatcgc ctacgacccc cccgccctgc aacttttggg acgtgccgcc gccggatcga
     5221 tgcgcgatgc cttaagcctg ctcgaccaag ccatcgccct aggttcgggc aaagttgccg
     5281 aaaacgatgt ccgccaaatg atcggcgcgg ttgacaaaca atacctgtac gaactgctga
     5341 caggcatcgt caaccaagac ggcgaagccc tgctggcaaa agcgcaggaa atggcggcgt
     5401 gtgccgtcgg ctttgacaac gccttgggcg aactggccat actgctgcaa caactcgccc
     5461 tgatacaggc agtcccctcc gccttggcgc acgacgaccc cgattccgat attttgcacc
     5521 gcctcgccca aaccataagc ggcgaacaaa tccagcttta ctaccaaatc gccgtccacg
     5581 gcaaacgcga cctgggcctc gcccccgacg aatacgccgg ctttatgatg accctgctgc
     5641 gtatgctggc gttcgccccc ttggcggcgg cttcgtgtga tgcaaacgcc gtgattgaaa
     5701 ataccgaact tcaatccccg tcggcacaaa ccgccgaaaa ggaaaccgcc gcaaaaaagc
     5761 cacaaccgcg ccctgaagcg gatgccgccc aaacgcccgt tcagacggca tccgcagcag
     5821 caatgccgtc tgaaggcaaa actgccggcc ccgtttccca tcaagaaaac aacgatgttc
     5881 cgccttggga agacgcgccg gacaaaaccg aaaccgcagc cggcacggcg cgaacgtcgg
     5941 caaaaagcat tcagacggca tccgaagccg aaacgccgcc cgaaaatcaa gtttccaaga
     6001 acaaggcagc cgacaacgaa accgaggctt ccttgtccga agtgccgtct gaaaacccca
     6061 ttcaggcaac accgaatgat gaagccgttg aaacggaaac atttgcacac gaagcccctg
     6121 cagaaccttt ctacggtttt ccggataatg actgcccccc ggaagacggc gtggaaatcc
     6181 cgccgcccga ttgggcaaat gtcctccctg ccgataccgc aggcggaggg acggacgaag
     6241 aagcagaagc gggcggcatc ggcggaaaca acacgccgtc cgtcccgccg cccgaatttt
     6301 ccaccgaaaa ctgggcagcc atcgtccggc acttcgcccg caaactcggc gcggcgcaaa
     6361 tgccggcgca acactccgcg tggacggaat accgttccga caccggactg atggttttgg
     6421 caatgaccgc cgaagcccgc gccaccgccg acaaaaagcg gctcgacaaa atccgcgaca
     6481 ccctcgccca agcctacggg ctgcaactca ccctgcaaac cgaagactgg cgcgacgaag
     6541 ccggccggga aacccccgcg atgcaggaca agcgtgtcca agccgaagac aggcaaaaag
     6601 cacaagcatt gctcgaagcc gaccccgccg cacaaaaaat cctccaagca ttcggcgcgc
     6661 aatggcagcc cgaatcactg gaattggcgg caaaccggcc ataaacagat ataatgccgc
     6721 ccgaaccctt cggacggcat tgccgcttcc ctcattcatt caaacacaaa caggagtatt
     6781 cagtatgttc ggaaaagccg gattaggcgg cctgatgaaa caggcgcagc aaatgcagga
     6841 aaacatgaaa aaggcacaag ccaaacttgc cgaaaccgaa atcgaaggcg aagcaggcaa
     6901 cggcttggtc aaaatcacaa tgacctgcgc gcacgaagta cgcaaaatcg acatcagccc
     6961 cgatttgatt caagaagccg ccgatgacaa agaaatgctc gaagacctca tcctcgccgc
     7021 cctcaaatcc gcccggggca aagccgaaga aaccgcaaac aaaacaatgg gcgcattcac
     7081 ccaagatctg ccccccggcg tgggcgactt cttccgctga ccccgaccgt cattcccgcg
     7141 aacgcgggaa tccggtaacc gaaaaaccac aggaatctat cggaaaaaaa cagaaacccc
     7201 cgccgccgtc attcccgcgc aggcgggaat ctagaacgta gaatctgaaa aaccgtttta
     7261 ctcgataaat ttccgtgccg acaggtctgg attcccgcct tcgcgggaat gacggcatat
     7321 tttttgcatt tgatataaag ggtcgtttga attttgttca gcaagtgcaa agtattgcac
     7381 ataaaagggc gcaggcttca tccgtaaagt cggcgcagga tagaggcaaa gcgggcgtag
     7441 gtcgggctgt agcaactgta tttttcaccc cgtcgggtaa aaatatagtg gattaacaaa
     7501 aaccggtacg gcgttgcctc gccttagatc aaagagaacg attctctaag atgctgaagc
     7561 accaagtgaa tcggttccgt actatagtgg attaaattta aaccagtacg gcgttgcctc
     7621 gccttgccgt actatttgta ctgtctgcgg ctctccgcct tgtcctgatt tttgttaatc
     7681 cgctatacca aaactcaaat caagccgtcc ggaggcggct caaaaaaaac ggtacttcgc
     7741 agcagaagta ccgtttatcg ggatttcagg ttttattctt cgggtcgttc gccgtcggtt
     7801 tcgtcctgcg tcccttcggt gatgtgcatt tctacgccgt tgagggcgcg gatttttgcg
     7861 tcgatttcgt cggaaatttc ggggttttct ttcagccaga cgcggacgtt gtctttgccc
     7921 tgaccgattt tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt
     7981 ttcacgccga tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca
     8041 aactctgcct gacggaacgg gggggcgact ttgtttttga tgactttgac gcgggtttcg
     8101 ttgcccaata cttcttcgcc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg
     8161 gaagaataga atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg
     8221 ccgatcttca ttcggatttg gttgatgaac acaaccagcg tattggtttt tttgatgtgt
     8281 ccggtcagtt tgcgcaaagc ctgactcatc aggcgcgcct gcagtccgac gtggctgtcc
     8341 cccatatcgc cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc
     8401 atatctatgc cgcccgaacg gacgagcgtg tcgcagattt ccaaagcctg ttcgccggta
     8461 tcgggctggg acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga
     8521 tcgaaggcgt gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg
     8581 acggcttcga ggcagagtgt ggttttgccg gaggattcgg ggccgaagat ttcgacgatg
     8641 cgcccgcgcg gcaggccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa
     8701 atgacttcga ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg
     8761 aaactttttt cgatttgcgc cagtgcggcg gcaagggctt tgcttttgtc gtctgacatg
     8821 ggggttactc cggaacaaat gcggtatgtg ggctgcggcg caacacgggc tgcggcgcgg
     8881 aatatgaatc ggtcggtcat tatcgcatgt ttgcctttcg ggggctacca tccgcttcag
     8941 accgaatgcc gtctgaaaca ttgtccctcc tttgctataa tggcgccctt acttttcccg
     9001 ccagccgctt gaaccgccat gcacaccagc ctgaccgtca aaaataccgt tatcggaagc
     9061 gggcgcacca aaatcgccgt gccgcttgtc gcccgagatg ccgccgattt atcctccgtg
     9121 ctgtcccaaa tcaaaaacct gcccttcgat atcgtcgagt tccgcgccga ctttttggaa
     9181 tgcgcgggca gcatcggcga agtattgcgc cacacgcagg ccgtccgcga cgcgctgccc
     9241 gacaaaccgc tgctgtttac gttcagacgg cattgcgaag gcggctcgtt cccgtgttcg
     9301 gacgattatt attttgaact gctcgacgcg ctgatcgaaa gccgcctgcc cgacattatc
     9361 gatatcgagc tgttttccgg cgaaaccgcc gtccgccgcg ccgtggcaaa tgctcaaaaa
     9421 aacggcatcg ccgccctgct ctgcaatcat gagtttcacc gcacgccgcc gcaagaagaa
     9481 atcgtatgcc gtctgaaaca gatggaggac tgcggcgcgg acatctgcaa aattgcggtg
     9541 atgccgcaaa gctcggaaga tgtgctgact ttgctttccg ccacgctcga agcgaaacgg
     9601 cttgtcgcca aaccggttat tacgatgtcg atggggcaga cgggtgcggt cagccggctt
     9661 gccggacagg tgttcggctc aagcatcacg ttcggttcgg gaacgcaaaa ctccgcgccg
     9721 gggcaaatcg gcgtatccgc cctccgtgcg gcactcgact gcctcgaaag cggcgcagac
     9781 tgatttcaga cggcatcaaa acatgatgaa actcaatgcc caacagctcg aagccgtccg
     9841 ctacctcggc ggccccctgt tcgtccttgc cggcgcaggc agcggcaaaa ccggcgtgat
     9901 tacgcaaaaa atcaagcatt tgattgtcaa tgtcggctat ctgccgcata ccgtcgccgc
     9961 gattaccttt accaacaaag ccgctgcgga aatgcaggag cgcgtcgcca aaatgctgcc
    10021 caaaccgcaa acgcgcgggc tgacgatttg cacgttccat tctttgggca tgaagattct
    10081 gcgcgaagag gcgaaccata tcggttacaa aaaaaacttc tccattctcg actctaccga
    10141 cagcgccaaa atcatcggcg aactcttagg cggcacggga aaagaagccg tattcaaggc
    10201 gcagcaccag atttcccttt ggaaaaacga tttaaaaacg cctgaagatg tcgttcagac
    10261 ggcctctaat gtgtgggaac aacaaaccgc gcgcgtgtat gcaagttatc aggaaaccct
    10321 gcaaagctat caggcagtag attttgacga cttaatccgc ctgcctgccg tgctgttgca
    10381 gcaaaacagt gaagtgcgca acaaatggca gcggcggctg cgttatctgt tggttgacga
    10441 atgccaagat acgaatacct gccaatttgc gctgatgaaa ctcttgacag gcgcggaagg
    10501 gatgtttacc gccgtcggcg acgacgacca gtccatctac gcatggcgcg gcgcgaacat
    10561 ggaaaacctg cgcaaaatgc aggaaaacta tccgcagatg aaggtcatca aactggagca
    10621 aaactaccgc tccaccgcgc ggattctcaa aattgccaac aaagtcatcg aaaacaaccc
    10681 caagctgttt accaaaaaac tttggtcgca attgggcgaa ggcgagccgg ttaaagtcgt
    10741 tgcctgccaa aacgagcagc acgaagccga ttgggtcgtc agccaaatcg tcaaacaaaa
    10801 actcatcggc ggcgacaaaa cccgatacgc cgattttgcc gtgttatacc gcggcaagca
    10861 tcaggcacgg attttcgagg aagcattgcg cggcgcgcgc atcccctacc gtctctccgg
    10921 cggacaaagc tttttcgaca aagccgaaat caaagacgtg ttgtcttatg tgcggctgct
    10981 tgccaacccc aacgacgatc ccgcctttct gcgcgccgtt accacgccaa aacgcggcat
    11041 cggcgatgta acgctgggca aactcaacgc ctacgcgcac gaacacgaat gcagcctgta
    11101 tgaagccgca caaaacgaag aagcccttgc cacactgaac aataccaacc gccaacacct
    11161 gcaagccttt atggatatgt tcggcaacta ccgcgccaaa gccgaaatcg acgaagcggg
    11221 cgagttcatc aacagcctgc tcgaagaaat cgactatgaa aaccatttga tgcaaaacga
    11281 agaaggcaaa gccggcgaaa tcaaatggcg caacgtcggc gagttggtat cgtggttcgc
    11341 acgaaaaggc gaacgggacg gcaaaaacgt catcgaactc gcccaaaccg tcgccttgat
    11401 gacgcttttg gaaggaaaag acgaagaaga aaccgatgcc gtctcgctat ccacgctgca
    11461 cgccgccaaa ggtttggagt atccgtatgt tttccttgtc ggttgcgaag aaggtgtttt
    11521 gccgcacaac gacagcatcg aagaaggcaa cgtcgaagaa gaacgccgcc tgatgtacgt
    11581 cggcatcacc cgtgccaaac gccaactcac gctgacccac tgtgtcaaac gcaaaaaaca
    11641 aggcatatgg caattccccg aacccagccg attcatagac gaaatgccgc aggaagattt
    11701 gaaaatcctg gggcgcaaag gcggcgaacc gattgtcagc aaagaagaag gcagacgtaa
    11761 ccttgccgat ataatcggaa ggatcgacaa cctaaaaaaa agcggcccgg cggattaaac
    11821 cggagccgca atgccgtctg aaagacgttc agacggcata ttttttgaac ggcgcgcgtc
    11881 aagcggttta cgcccacaaa tcctgctgct gatttttcgg tacaagcctg ccgacaccga
    11941 taccgatgag gcggaacgcg tcttccgtct gcggcgggac gcgcgccatc aacatttgcg
    12001 cagcctgcag cagagctgcg cagtcgggca atacggagga ataagtcagc gtgcgcgtga
    12061 tgatgcggaa atcgtaggtc ttcagcttga gcgttacgct ttgggcttcg acgtttttgc
    12121 gcgtgatttg ccgccacagg tcttcggcaa gatggggcag gtgtccggca gcctgcccga
    12181 gcggcaggtc ttcgggcagg gtgatttctg tggaaatttg gaggcgttcg cgttcggctt
    12241 tgacggggtg ttcgtccgta ccgcgcgcca aatcatagag gcggtagccg tagcgtccga
    12301 aatgatttaa gagctcgccg cgctcgaaac ggcgcaaatc gcccgccgtc cgcataccca
    12361 gcgactgcat ttttttcagc gttaccttgc ccgctccggg gattttgccc aaaggcaggg
    12421 tttccaaaaa tgccataact ttgtgcggcg gcaacacaaa ctgcccgttc ggcttgcgcc
    12481 agtcggaagc aattttcgcc agaaatttgt tcggcgcgat gcctgcggat gcggtcaaac
    12541 ccgtttccgc aaaaatggcg gcacggattt ctttggcaac ttcgccggcg taagggatat
    12601 ttttgaaatt acgggtaacg tcaagatagg cttcgtccgg cgacaagggt tcgattaaat
    12661 cggtataacg cctgaatacg gcgtgaatct gcgcggaaac ctgacggtac aaatcgaaat
    12721 gcggaggcac atacaccgct tgcggacaca gccttttcac cgttgccacc gacatcgcgg
    12781 aatgcagccc gaactgccgt gcctcatacg atgcggcgca aatcaccgaa cgcgcgccct
    12841 cccacgcgac gaccaccggc cgccctttca aatgcggctg ctcgcgtagt tctaccgatg
    12901 cgtagaacgc gtccatgtcg atgtggatga ttttgcgtaa agacatcggc tctgccgagg
    12961 ataaaaggga tattttactg cgggcatcgg acaaaatcca aatatactaa caggttaaat
    13021 acaaaagagt tggctggatt aattttgaaa ctcgataaaa ataatggttt ttatcaagca
    13081 gaaagaaaca gtaatggaaa ttatgatatc gtaagaaaaa attatctgaa aaatattacc
    13141 aaaatacaat caaatatatt gatttactta gataatacca gtaccggatt taaaattcaa
    13201 cagaggagaa aacaaatcag a
//
LOCUS       DAXFWM010000068        12699 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000068 DAXFWM010000000
VERSION     DAXFWM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.068"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..803
                     /locus_tag="ACFI8B_001795"
     CDS             <1..803
                     /locus_tag="ACFI8B_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFC3834950.1"
                     /translation="KNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKP
                     EYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNENDIVLDYHLGSGTTAAVAH
                     KMNRQYIGIEQMDYIETLAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETA
                     KQQILACENSDGIKTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLE
                     MLDLNQMYISLSEMDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            793..3567
                     /locus_tag="ACFI8B_001796"
     CDS             793..3567
                     /locus_tag="ACFI8B_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC3834951.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVILGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENAEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(3716..4888)
                     /locus_tag="ACFI8B_001797"
     CDS             complement(3716..4888)
                     /locus_tag="ACFI8B_001797"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC3834952.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(5009..5251)
                     /locus_tag="ACFI8B_001798"
     CDS             complement(5009..5251)
                     /locus_tag="ACFI8B_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834953.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            5238..5684
                     /gene="iscR"
                     /locus_tag="ACFI8B_001799"
     CDS             5238..5684
                     /gene="iscR"
                     /locus_tag="ACFI8B_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC3834954.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            5713..6927
                     /locus_tag="ACFI8B_001800"
     CDS             5713..6927
                     /locus_tag="ACFI8B_001800"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC3834955.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            6912..7223
                     /locus_tag="ACFI8B_001801"
     CDS             6912..7223
                     /locus_tag="ACFI8B_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834956.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            7529..8023
                     /locus_tag="ACFI8B_001802"
     CDS             7529..8023
                     /locus_tag="ACFI8B_001802"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFC3834957.1"
                     /translation="MQPITGNFDLAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFA
                     NFAFIENESRKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLF
                     MTQLAIKNGFQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQ
                     TTIF"
     gene            8036..8422
                     /gene="iscU"
                     /locus_tag="ACFI8B_001803"
     CDS             8036..8422
                     /gene="iscU"
                     /locus_tag="ACFI8B_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC3834958.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8509..8829
                     /gene="iscA"
                     /locus_tag="ACFI8B_001804"
     CDS             8509..8829
                     /gene="iscA"
                     /locus_tag="ACFI8B_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC3834959.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8789..9010)
                     /locus_tag="ACFI8B_001805"
     CDS             complement(8789..9010)
                     /locus_tag="ACFI8B_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC3834960.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            9092..9592
                     /gene="hscB"
                     /locus_tag="ACFI8B_001806"
     CDS             9092..9592
                     /gene="hscB"
                     /locus_tag="ACFI8B_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC3834961.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            9690..12440
                     /gene="gyrA"
                     /locus_tag="ACFI8B_001807"
     CDS             9690..12440
                     /gene="gyrA"
                     /locus_tag="ACFI8B_001807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC3834962.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT         3851 a         3193 c         2780 g         2875 t
ORIGIN      
        1 taaagaataa ggcaaggctg cagacggata ttattgttag ccaatctaaa aatggaattt
       61 ccttgtataa aaaacaaaga cctgaattag atgatttacc tacaaaaaaa cctaaaacta
      121 ttttttataa acctgaatac agtagtggta atggaactga acagatgaaa aatctttttg
      181 gtgaaaaggc atttaaaaat cctaaaccag aagaacttat ccaagatttt attaccatca
      241 caaccaacga aaacgacatc gttctagact accacttggg cagtggcaca accgccgccg
      301 tcgcccataa aatgaaccgc caatacatcg gcattgaaca aatggattat attgaaacgc
      361 ttgccgttga acgcatgaaa aaagtaattg acggcgagca aggcggtatc tcaaaagctg
      421 tgaattggca aggtggcggc gagtttgttt atgccgaact ttccccattt aacgaaaccg
      481 caaaacaaca aattttggcg tgcgaaaatt cagacggcat caaaacgctg tttgaaggtt
      541 tatgcgaacg ccatttcttg aaatacaacg tcagcgtaaa tgaatttagc caaattattg
      601 aagagcctga atttcaatcc ttggcattag acgaacaaaa acaaatgatg cttgaaatgt
      661 tggatttaaa tcaaatgtat atttcattat ccgaaatgga cgatgaacaa tttgcaggct
      721 gcctgaatga tgatgataaa gcgttaagcc gtgcgttcta tcaatcagta aaacatcaag
      781 cggagaaaaa agatggcgaa taataaaacg ttgtttgatt gggtggaaga ccgcaaatca
      841 atgctggaag aaatggaaca gacggatttt ttcgcgctgc ctgaatttgt ttccaataat
      901 ttaaaatatc cgttttttga atggcaaaaa tcggctttag aaaactttgt gatttttgac
      961 cgcacttcaa aattaaagga tttccccgac atcaaaaacc gccctaccca tttgctgttc
     1021 aatatggcaa caggtgcagg caaaacgatg atgatggcgg cattgattct gtattatttt
     1081 gagaaagggt atcggcattt tttatttttc gtcaataaaa acaatatagt atataaaacg
     1141 gaaaataatt ttatcgaccc gacgcatcca aaatttttat ttaccgagaa aattttgcaa
     1201 ggtgatacgg taattcccat tcgcaaagtg gagacattta gccaatattc agacggcatt
     1261 gaaattaaat ttaccagcat tcaaaaacta tataacgata ttcacaccga gcgagaaaat
     1321 caaaccacat tggcggattt gcacgaatta aatcttgtga ttctgggcga tgaagcacac
     1381 catttaaacg cgcaaaccaa aggcaaaaaa caaggcgaat tagatttaga aaaggaaatg
     1441 aacgagcgta ccagcgatgc cgaaattgaa cgcaaaggct gggagcatat ggttttggag
     1501 ttgttactca ataaaaatgg caatcccggc caaaatgtgc tgttggaatt taccgccaca
     1561 ctgcctgaaa atgctgaagt gcaacaaaaa tatgctgata aaatcatcac aaaatttggt
     1621 ttaaaagaat ttttgcaaaa aggttatacc aaagaaatca atttggtatc cggtacgctg
     1681 aataagaaag agcgggtgtt gcacgcttta ttgtttgcct ggtaccgcca tcaaatcgcg
     1741 ttgaaatacg gcattgccaa tttcaagcct gtgatgttgt ttagaagtaa gacgattgat
     1801 gaatcaaaag cggattattc ggcgttttta aattgggtag aaaatgtgca agcggatgat
     1861 ttttcgtttt taactacatt ttcaacaagc ttaaacgata gcaataacgc caacgaacaa
     1921 ggcaaaaccc gtaccgaaca agccctgaaa tttatgcagg aaaataaatt tgaatttgtg
     1981 catttggcaa attggattaa acaaaattat caaaaacaca atgtcattat taccaactcc
     2041 gaaaccaaca aaaacaaaac tgaaaaaacc gacagcgaaa ccgaaaaatt gctaaacaat
     2101 ttggaagcgg ctgataatcc gatccgtgcc atttttacgg tggatagatt aaccgaaggt
     2161 tgggacgttt taaatttgtt tgatattgtg cgtttgtatg aagggcaaaa cggtggcggt
     2221 tcgaataaaa aatcgggcaa aacggctgcc gccaccgtat cggaaaagca gttgattggt
     2281 cgcggcgtgc gttattttcc atttgcgttt gaaggtaaac agccgaataa acgcaaattt
     2341 gacaacgata tgcaacatga attgcgtatc ttggaagaat tgttttatta cacgcacgat
     2401 gagcaatccc gctatattac agaactgaaa aacgagttac gaaaagacgg ttatttgcct
     2461 gaaaaagacg atgataaggt attggcaaca tttaagctca aatctgaatt tgcagataat
     2521 aaggatttta gagagttgtt aatttgggcg aataaaaaaa tccccaaccc aaacgccaaa
     2581 agcaataatg cagacagcct gcaagccaat ccgcaaacgc tttcattcca agttcacggt
     2641 aatcaactgt tgcaggaaac gcaatttaca gccgatgaaa atgatgaaac agcccgacaa
     2701 atcggcacac aaaataattt tactcaaacc ataaaaatga gtgaaatgga gcggcatatt
     2761 ttcaataaag cattacatat caaagggaaa aatagtcaat ctttattcca tttcgaccgc
     2821 ctgcaaagca aactcgacat tcaaaatcgc aatgaattgc aaaataaatt gttgaaagac
     2881 tggcagattg aatttttggg attagagcaa gataaacagg ttcgcccgga tgataaactt
     2941 gcaggctgcc tgaaaatctt ggaaatggtt gaaaaacatc tgaatgaaag tgatataccg
     3001 tttatcggta caaaagaatt tacacctaaa aaattgtggg aaattttcgg cacgccaaaa
     3061 caaaaatggg tcaaaaaaga tgatataaaa actgctattg ccacgcaaaa tgattggtat
     3121 gtgatggata attttgccgg aacgggtttg gaagaagcgt taattcaatt tatttcagcg
     3181 cgcttgggcg atttgaaatc tcaatatgac gttcatttaa tccgcaatga agaagtgttt
     3241 aaattgaata actttgccga tggcgaagga tttatgccgg actttgtttt attgctgaaa
     3301 gataaacaaa aatcttcttc cgacagcgtg gacgactttt tgcattacca aattttcatt
     3361 gaaccgaaag gcgggcattt agtggaaaat gattcgtgga aagaagcttt tttaaaatca
     3421 atcacagtgg aatatgggag ggataagatc ctgcaaaaaa atacaccgca ttatcgtttg
     3481 atcggtttgc cgttttttac tgatcatcag aaaaatggac aatttacaga gttattccct
     3541 ttaggtgcgg catcgcttga aaaatagagt agtgcattgc aggcagcccc gttcggcaaa
     3601 acttccttta caaaagggcg ttttgtcaga tatttaatca acacattatt aaaatacaac
     3661 caaattttaa tgccgtccga accatgtatt cagacggcat cgtatttttc agtatctaaa
     3721 ccgtttccct gccccaatcc ttactcctca aaatcgaggc atcgacatct tgaatatcgc
     3781 ggtgtcccgt aaacgccatc gacacatcca tttccttgta caaaatctcc agcgcgcgcg
     3841 ttacgccttc ttcgccatac gcacccaaac catacaggaa cgcccgacct atcatcgtgc
     3901 ctttcgcacc caaagcccac gccttcaaaa tatcctggcc gctgcggata ccgctgtcca
     3961 tccaaacttc gatgtcgctg ccgactgcgg aaacaatatc gggcaaggcc ttgatggcgg
     4021 acacagtatc gtcgagctgg cggccgccgt ggttggaaac gaccaatgca tccgcaccgc
     4081 ttttcgccgc cttttccgcg tcttcgggtt ccataatgcc tttgataatc agcttgccgc
     4141 cccacaaatc tttaatccgc gccacgtcgt cccagcttaa gcgcgggtcg aactgttccg
     4201 ccgtccacga agaaagtgaa gacaaatcgc cgacgttttt ggcgtgtccg acgatattgc
     4261 ggaacgtgcg gcgttccgtg ttcagcattt tcatgcacca ttcgggcttg gtcgccagat
     4321 tgattaaatt ggcgatggtc ggtttcggcg gcgcggacag gccgtttttg atgtctttgt
     4381 ggcgttgacc caaaacctgc aaatcggcag tcaataccaa tgccgaacat ttggcatctt
     4441 tcgcacgctt aatcaggttt tccataaatt cgcggtcgcg catcacataa agctgaaacc
     4501 aaaacggcgc gctggtgttc tcggcaacgt cttcaatcga gcagatggac atggtggaca
     4561 acgtaaacgg aataccgaac ttctccgccg cacgcgccgc caaaatttcg ccgtctgcgt
     4621 gtgccatgcc ggtaaaaccc gtcggcgcaa tcgccaccgg cattttcaca tcccccccga
     4681 tcattttggt ttcgaggctg cgtccctcca tattgaccaa taccttttgt cggaagcgga
     4741 tatctttgaa atccgaagta ttctccctat aagtcgtttc cgtccacgaa cccgaatcga
     4801 tgtaatcgta aaacatacgc ggcattttgc gcttggcaac gcggcgcaag tcttcgatgc
     4861 aggtcatttt gctcaaatca cgtttcatct tgtttttccc tgtttaacag cctgatggaa
     4921 tatgccgtct gaaactgctt gagccttcaa acggcatttc gggcaatacc caaataaatc
     4981 actaagattt agataatcta tatcaatatt aaaaataaat caaatcgttt tgaaaccgct
     5041 tgccggcaaa acgccccaac cgccgctcct caaaaaacat aactaactga ttcaaaaaac
     5101 caaacaaata aagcaaaatc agactatacc gccatttaaa caaacaatcg gcaacagctc
     5161 ccccatactt gactgaaaca ctcagatatt ggacaattcc gcccactata aaaaagccga
     5221 cacgggcaac caccaccatg agactgacca ccaaagggcg tttcgccgtt accgctatgc
     5281 tggatttggc gatgaacgcg caaaccggcg ccgtcaaact cagtgccatc agcgaacgcc
     5341 aaaatatatc cctctcctat ctcgagcaat tgttcggcaa actccgccgc gccggactgg
     5401 ttgaaagcct gcgcgggccc ggcggcggct acatcctcgc cgcccctccc gcacaaatca
     5461 acatcgccca aatcatttcc gccgccgaag accgtctgga cgcgacccaa tgcggcagca
     5521 aagccaactg ccaccacggc gcgccctgcc tgacgcacga cctttgggag aatttaaaca
     5581 aaaccatcaa cgactatctc ggcagcgtta ccctgcaaag catcatcgaa cagaaaaaca
     5641 acggcgacgg cagccgcgtc gtccaattta cacacatcca ttaaataaca cccgaaaaag
     5701 aaagagcaaa ccatgaccgt caaaaccccc gtttacctcg actacgccgc caccacaccc
     5761 gttgacaaac gcgttgccga aaaaatgatt ccctatctga ctgaaacctt cggcaacccc
     5821 gcctccaaca gccacgcatt cggctggacg gcagaagaag ccgtcgaaaa agcccgcgcc
     5881 gacatcgccg ccctgattaa cgccgacccc aaagaaatcg tcttcaccag cggcgcgacc
     5941 gagtccgaca acctcgccat caaaggtgcg gcaaacttct acaaaaccaa aggcaaacac
     6001 ctcatcaccg tcaaaaccga acacaaagcc gtgctcgaca cgatgcgcga actcgaacgc
     6061 caaggctttg aagttaccta cctcggcgtg caggaaaacg gtttgattga tttggaagaa
     6121 ctcaaagccg ccatccgcga cgacaccatc ctgatttcca taatgtgggt gaacaacgaa
     6181 atcggcgtgg tgcaaaacat tcccgccatc ggcgaaatct gccgcgaacg caaaatcgcc
     6241 ttccacgtcg atgccgccca agcctgcggc aaagtgcctg tcgatgtcga agccgccaaa
     6301 atcgacttgc tctcgatgtc cgcgcacaaa gtgtacggcc ccaaaggcat cggcgcgctg
     6361 tacgtccgcc gcaaaccgcg cgtccgcctc gaagcccaaa tgcacggtgg cggtcacgag
     6421 cgcggtttcc gttccggcac attgccgacc catcaaatcg tcggcatggg cgaggccttc
     6481 cgcatcgcca aagaagaatt ggcacaagac acagcgcact acctgaaact gcgcgacatc
     6541 ttcctcaaag gcatcgaagg catcgaagaa gtctatatca acggcgacct cgaacaccgc
     6601 gccccgaaca acctgaacgt cagcttcaac ttcgtcgaag gcgaaagcct gattatggcg
     6661 gtgaaagaac tcgccgtatc cagcggatcc gcctgcacct ccgcctcgct cgaacccagc
     6721 tacgtcctgc gcgcgcttgg ccgcaacgac gaactggcgc actcatccct gcgcatcacc
     6781 ttcggccgca tgaccaccga agaagaagtg caattcgcgg cagaactgat caaatccaaa
     6841 atcggcaaac tgcgcgaact gtcgccgctg tgggaaatgt tcaaagacgg tattgatctg
     6901 aactcgattg aatgggcggc gcattaagat tgatgtcaaa caaaggctgt ctgagaaaga
     6961 ataccatgaa agttaaaatt attaatttgc caaatggtta taaacgtatt atttatggaa
     7021 aatattttga acaatttgac ttagattatg aacaagattt agacgtatta aaaaaagata
     7081 ttgaatttgc tttatctgtt attgaataca atcgatctat attcaaaaaa ttctcttcct
     7141 tatttgagaa taaaatcatt tttgtttatc aaggaggaca tcatcttgat attattgatc
     7201 gtgataaagg aagtttgaaa tagttaacta ttgaagatca ttggacaaaa gatgttgatg
     7261 atatcactgg ttcatggaaa atggagaatt tagaaattag ccgacaaact caatatgcaa
     7321 tgcaacttct aaagattggt aaaacaaata ttgagaaaga aataagaaaa attaaacaaa
     7381 ggttctaaaa aaatgagtaa atacggtggt gaagacgtaa tctatatgaa tgatggtgta
     7441 tttagaaata aattagccat tcaagatcca cttaaattta aaaaaggtcg aaagagacat
     7501 ttctgctatt tccgcccaaa atctgctttt gcaacccatt acaggcaatt tcgatttggc
     7561 gcatttacag accattcatc gcgaactgtt tggcaatgtt tatgattggg caggaaaaat
     7621 ccggagggtt gatatttcta aaggtaatac ccgatttgcc aattttgctt ttatcgaaaa
     7681 cgaaagccgt aaacttttgg agaagctgaa aaacgaaaac tatttgcggg tgttggataa
     7741 agataaattc gccgagcgtg cagcctatta tttggacgag ttgaacgtcc tacatccttt
     7801 tcgtgaagga aacggcagaa cattgcgcct gtttatgaca caattggcaa taaaaaacgg
     7861 ttttcagata cattggcaaa atatctctgc cgaacaaatg attcaagcct gtattcaggc
     7921 ataccatgcc gatagcagct tattggcacg cctaattatt agacaattta gaacaatcgt
     7981 ctttttcaga cgactaaaac atcaacaaac cacgatattt taaggaaacc acatcatggc
     8041 atacagcgat aaagtaatcg accactacga aaatccccgc aacgtcggca ctttcgacaa
     8101 aaacgacgag tccgtcggca ccggcatggt cggcgcgccc gcctgcggcg acgtgatgcg
     8161 cctgcaaatc aaagtgaacg atgaaggcat catcgaagat gcgaaattca aaacttacgg
     8221 ctgcggttcc gccatcgctt cgtccagcct gattaccgag tgggtcaaag gcaaaagtct
     8281 ggatgacgcg ctggcaatca aaaacagcga aatcgccgaa gaactggaat tgccgccggt
     8341 aaaaatccac tgctccatct tggctgaaga tgcggtaaaa gcggccgttg ccgactaccg
     8401 caaacgtcag gaaaacagat aaagcccttc agacggcatc gtcccacaat gccgtccgaa
     8461 ccgcccgccg cttcggatcc gtccggggcg gtacaacaag gaagaaatat gattaccctt
     8521 accgagaatg ccgcaaaaca catcaatgac tatctcgcca aacgcggcaa aggcttgggc
     8581 gtacgcttgg gtgtaaaaac cagcggctgc tcggggatgg cgtacaacct tgaatttgtc
     8641 gatgaagcca acggcgacga cctgattttc gaaggacacg gcgcgcgcat ttatatcgac
     8701 ccgaaaagct tggtttatct ggacggcaca caagtcgatt acaccaaaga agatttgcag
     8761 gaaggtttca aatttgaaaa ccccaatgtc aaagactcct gcggctgcgg cgagagcttc
     8821 cacgtttaag gcataaaaac ggcgggacag tatcaaaacc gtcccgccgt tttttcgctt
     8881 cctgcctgtt gtagctgcct ttgcctttcc ttttccgttc caccttgtgc cggaacaaat
     8941 cggattttac taaggctttt aaagcattgt cgcgtatttt gcctttattg tgctgcgctt
     9001 tgccgcccat attcagtcct ttcgtttaag aagccgcaga ttataaggca aaaacagttt
     9061 tctgccaaaa tcttacattt atcatcctac tatgtcccaa tatttcaccc tcttccggat
     9121 tgaacccgct ttcgatatcg gcaccgaaaa cttggaacaa acctaccgcg ccttggccgc
     9181 ccgtttccat cccgacaaat tcgcttccgc ttccgctttc gagcagaaac aggcggttat
     9241 gatgtcttcc accatcaacg atgcctaccg caccttgaaa aatcccatcg accgcgccgc
     9301 gtacctgctg aaaacatcgg gtatcgatgc cgacgcgccg gaacatacct ctttcgcccc
     9361 cgacttcctc atgcagcaaa tggaatggcg cgaaacgctg atggaggcac gggcaggcaa
     9421 caaccttgaa tccttgaaaa atctcgataa cgaaatccgc gccgaacaag aaaaactgtt
     9481 ctgcggtctg aaacagtcat tcgcgcgcca agactgcgac accgccgcac aacaagtccg
     9541 ccaaggcagg tttctcgaca aactccgcca cgaaatttcc tccgcattat aatccgcacc
     9601 gtgtttcaga cggcataacc gccgcaccgt ttcatgtcga aatatgctaa aataagcaac
     9661 aattttttgc catacgaaac attgaaacca tgaccgacgc aaccatccgc cacgaccaca
     9721 aattcgccct cgaaaccctg cccgtcagcc ttgaagacga aatgcgcaaa agctatctcg
     9781 actacgccat gagcgtcatt gtcgggcgcg cgctgccgga cgttcgcgac ggcctaaagc
     9841 cggtgcaccg gcgcgtactg tacgcgatgc acgagctgaa aaataactgg aatgccgcct
     9901 acaaaaaatc ggcgcgcatc gtcggcgacg tcatcggtaa ataccacccc cacggcgatt
     9961 ccgcagttta cgacaccatc gtccgtatgg cgcaaaattt cgctatgcgt tatgtgctga
    10021 tagacggaca gggcaacttc ggatcggtgg acgggcttgc cgccgcagcc atgcgctata
    10081 ccgaaatccg catggcgaaa atctcacatg aaatgctggc agacattgag gaagaaaccg
    10141 ttaatttcgg cccgaactac gacggtagcg aacacgagcc gcttgtactg ccgacccgtt
    10201 tccccacact gctcgtcaac ggctcgtccg gtatcgccgt cggtatggcg accaacatcc
    10261 cgccgcacaa cctcaccgac accatcaacg cctgtctgcg tcttttggac gaacccaaaa
    10321 ccgaaatcga cgaactgatc gacattatcc aagcccccga cttcccgacc ggggcaacca
    10381 tctacggctt gggcggcgtg cgcgaaggct ataaaacagg ccgcggccgc gttgttatgc
    10441 gcggtaagac ccatatcgaa cccataggca aaaacggcga acgcgaagcc atcgttatcg
    10501 acgaaatccc ctatcaggtc aacaaagcca agttggtcga gaaaatcggc gatttggttc
    10561 gggaaaaaac actggaaggc atttccgagc tccgcgacga atccgacaaa tccggtatgc
    10621 gcgtcgttat cgagctgaaa cgcaacgaaa atgccgaagt cgtcttaaac caactctaca
    10681 aactgactcc gctgcaagac agtttcggca tcaatatggt ggttttggtc gacggacaac
    10741 cgcgcctgtt aaacctgaaa cagattctct ccgaattcct gcgccaccgc cgcgaagtcg
    10801 ttacccgacg tacgcttttc cggctgaaga aggcacgcca tgaagggcat atcgccgaag
    10861 gcaaagccgt cgcactgtcc aatatcgatg aaatcatcaa gctcatcaaa gaatcgccca
    10921 acgcggccga ggccaaagaa aaactgcttg cgcgcccttg gcgcagcagc ctcgttgaag
    10981 aaatgctgac gcgttccggt ctggatttgg aaatgatgcg tccggaagga ttggctgcaa
    11041 acattggtct gaaaaaacaa ggttattacc tgagcgagat tcaggcagat gctattttac
    11101 gcatgagcct gcgaaacctg accggcctcg atcagaaaga aattatcgaa agctacaaaa
    11161 acctgatggg taaaatcatc gactttgtgg atatcctctc caaacccgaa cgcattaccc
    11221 aaatcatccg tgacgaactg gaagaaatca aaaccaacta tggcgacgaa cgccgcagcg
    11281 aaatcaaccc gttcggcggc gacattgccg atgaagacct gattccgcaa cgcgaaatgg
    11341 tcgtgaccct gacccacggc ggctatataa aaacccagcc gaccaccgac tatcaggctc
    11401 agcgtcgcgg cgggcgcggc aaacaggcgg ctgccaccaa agacgaagac tttatcgaaa
    11461 ccctgtttgt tgccaacacg catgactatt tgatgtgttt taccaacctc ggcaagtgcc
    11521 actggattaa ggtttacaaa ctgcccgaag gcggacgcaa cagccgcggc cgtccgatta
    11581 acaacgtcat ccagctggaa gaaggcgaaa aagtcagcgc gattctggca gtacgcgagt
    11641 ttcccgaaga ccaatacgtc ttcttcgcca ccgcgcaggg aatggtgaaa aaagtccaac
    11701 tttccgcctt taaaaacgtc cgcgcccaag gcattaaagc catcgcactc aaagaaggcg
    11761 actacctcgt cggcgctgcg caaacaggcg gtgcggacga cattatgttg ttctccaact
    11821 tgggcaaagc catccgcttc aacgaatact gggaaaaatc cggcaacgac gaagcggaag
    11881 atgccgacat cgaaaccgag atttcagacg acctcgaaga cgaaaccgcc gacaacgaaa
    11941 acaccctgcc aagcggcaaa aacggcgtgc gtccgtccgg tcgcggcagc ggcggtttgc
    12001 gcggtatgcg cctgcctgcc gacggcaaaa tcgtcagcct gattaccttc gcccctgaaa
    12061 ccgaagaaag cggtttgcaa gttttaaccg ccaccgccaa cggatacgga aaacgcaccc
    12121 cgattgccga ttacagccgc aaaaacaaag gcgggcaagg cagtattgcc attaacaccg
    12181 gcgagcgcaa cggcgatttg gtcgccgcaa ccttggtcgg cgaaaccgac gatttgatgc
    12241 tgattaccag cggcggcgtg cttatccgta ccaaagtcga acaaatccgc gaaaccggcc
    12301 gcgccgcagc aggcgtgaaa ctgattaact tggacgaagg cgaaaccttg gtatcgctgg
    12361 aacgtgttgc cgaagacgaa tccgaactct ccggcgcttc tgtaatttcc aatgtaaccg
    12421 aaccggaagc cgagaactga aaatcatctc ccgatgccgt ctgaagattc agacggcatt
    12481 tatttttccc tcatccgtca tccagcttct cacaatatag cggattaaca aaaatcagga
    12541 taaggcgacg aagccgcaga cagtacaaat atagtggatt aaatttaaac tagtacagcg
    12601 ttgcctcgcc ttgccgtact atagtcaatt aaaaacaagg ggctgtacta gattatccct
    12661 aaattccaca ccgatcccgc aggattttta gctgccggg
//
LOCUS       DAXFWM010000069        12170 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000069 DAXFWM010000000
VERSION     DAXFWM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.069"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(69..2045)
                     /locus_tag="ACFI8B_001808"
     CDS             complement(69..2045)
                     /locus_tag="ACFI8B_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC3834963.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLECGDVLVVAGKKEEIISFENWSLQGI"
     gene            2191..3030
                     /locus_tag="ACFI8B_001809"
     CDS             2191..3030
                     /locus_tag="ACFI8B_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC3834964.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3229..4260
                     /gene="gap"
                     /locus_tag="ACFI8B_001810"
     CDS             3229..4260
                     /gene="gap"
                     /locus_tag="ACFI8B_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC3834965.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4515..5240)
                     /gene="aat"
                     /locus_tag="ACFI8B_001811"
     CDS             complement(4515..5240)
                     /gene="aat"
                     /locus_tag="ACFI8B_001811"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC3834966.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5310..5744)
                     /gene="fur"
                     /locus_tag="ACFI8B_001812"
     CDS             complement(5310..5744)
                     /gene="fur"
                     /locus_tag="ACFI8B_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC3834967.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5971..6348
                     /locus_tag="ACFI8B_001813"
     CDS             5971..6348
                     /locus_tag="ACFI8B_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC3834968.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            6358..7167
                     /gene="dapB"
                     /locus_tag="ACFI8B_001814"
     CDS             6358..7167
                     /gene="dapB"
                     /locus_tag="ACFI8B_001814"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC3834969.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7281..7550)
                     /locus_tag="ACFI8B_001815"
                     /pseudo
     CDS             complement(7281..7550)
                     /locus_tag="ACFI8B_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7641..7799)
                     /locus_tag="ACFI8B_001816"
     CDS             complement(7641..7799)
                     /locus_tag="ACFI8B_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834970.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(7807..8979)
                     /gene="lpxB"
                     /locus_tag="ACFI8B_001817"
     CDS             complement(7807..8979)
                     /gene="lpxB"
                     /locus_tag="ACFI8B_001817"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC3834971.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9130..10122)
                     /locus_tag="ACFI8B_001818"
     CDS             complement(9130..10122)
                     /locus_tag="ACFI8B_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC3834972.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYIGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
     gene            10615..>12170
                     /locus_tag="ACFI8B_001819"
     CDS             10615..>12170
                     /locus_tag="ACFI8B_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HFC3834973.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKA"
BASE COUNT         2981 a         3957 c         2856 g         2376 t
ORIGIN      
        1 tgcgatttgc gaaaacgcta ttagaccaaa taaccgggaa tggcgcaagc cttatttcag
       61 catttcattt atattccctg caagctccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgcattcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcccaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ccatatcggt aaacgttttc acataatcgt cattggtggc gcgcgcatat
      541 acgggcatat tgggatgcat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggc tcgcctgcac ttctggctac ctgcacgcgt
      721 gcgatgtcca agtcgagcgc gaaatacgga atatcctctt gggcgaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatgat cagacttgct catcgcttcc
      841 accaacatgc tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caagcgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccaa ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgct tcattttgaa tgcaatggca aacacaacca atgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa gaagccgagc aaaatatcgc ggaacgggcg aatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcaacatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgccagcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaact ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg atacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gaccgaacac cagccgcctc
     1801 atcgccctca gcttgggcaa tgaaaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaga acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatggacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggtg cggcatacaa ttctgataaa
     2161 atacgccctt ttgacccgca ccaccgcaca atgaccgcca ccgccgccga tttagaccga
     2221 ctcctccccc aaacccaatg ccgcgaatgc ggctatgaag gctgcctgcc ctacgcccgc
     2281 gcgatgctgc ggggcgaggc gcacaacctc tgcgccccgg gcggcgcaac cgtcgtccgc
     2341 gaccttgccg ccctgctcgg caaacccctt gtcgcacctg ccaaaaccca agccaaagca
     2401 ctcgcccgga tagacgaaac cgcctgtatc ggctgcaccg cctgcatccg cgcctgccct
     2461 gccgatgcca ttatgggcgc gggcaaactt atgcacaccg tcatcaccga cgaatgcacc
     2521 ggctgcggac tctgcgtcgc cccctgcccc gtggactgca tccatatgca gcccgttgcc
     2581 gacaccgtcc tgccgcgcgc gcgccgtttc agcctgtccg acgacagccg ttttgccgcc
     2641 gccgaacacg cgcgtgcgcg ctacctcaaa cgcaacgaac gcaaacagcg cgaagccgac
     2701 gaacgcaagg ctatgcttgc cgaacgcgaa gccgccgtcc gcaacgcgcg tccgcaaacg
     2761 cccgacacac cgaaaaaacc ggcgttcaac cccgccgacc tcatcgccaa agccatggca
     2821 aaagcgcaaa cccaacagga tcgcctcgcc gccgccgaca accgccaagg ctatcaggca
     2881 aaacagatag ccgaagcccg cgaacgcgcc gaattgcgcc gcgcccaacg cgatatgaaa
     2941 tacggcagcg acagcgaaaa agccgccgcc ctcgaatacc tcaaacaata caaagccaaa
     3001 caggaagccg cacagaatac cgcctcctga cccttacccg acatgccgtc tgaagccgct
     3061 tcagacggca ttttatcaag ctctccgtcc gccgtacccg tgccgtccgt atcttaccgc
     3121 ccacccttcc ggccgtgccg ttttcaataa aatattaatt acacgccact acaaatttgc
     3181 tataatccgc cccgaaaatc tacccaaccc tcaacaaagg aacaaaccat gggcatcaaa
     3241 gtcgccatca acggctacgg acgcatcggc cgccaggttt tgcgcgccat ctacgactat
     3301 cagattcaag accaactcca aatcgtcgcc gtcaacgcca gcggcagcct tgaaaccaac
     3361 gcccatctga ccaaattcga caccgtacac ggacgctttg aagccgacgt atcccacgac
     3421 ggcggcaacc tcatcgtcaa cggcgacaaa atccccttct tctcgacccg caaccctgcc
     3481 gaactgccgt ggaaagaact cggtgtcgat ttggtcatgg aatgcaccgg cgcgttcacc
     3541 agcaaagaaa aagccaaaat ccacctcgaa agcggcgcga aaaaagtcct catttccgca
     3601 ccgggcggcg acgatgtcga tgcaaccgtc gtgtacggcg tgaacgacag cgtcctgacc
     3661 gccgacatga ccgtcgtttc caacgcttcc tgcaccacca actgcctctc gccggttgcc
     3721 aaagtgttga gcgaaagcgt cggcatcgtc aaaggcgcaa tgaccaccat ccacgcactg
     3781 accaacgacc aaaccgttac cgacgtgcgc cacaaagacc tgcgccgcgc ccgcagcggc
     3841 gtggaaaaca tgattccgac caaaaccggc gcggcaaaag ccgtcggttt ggtcttgccc
     3901 gaattgaaag gcagactcga cgggcttgcc atccgcgtgc cgaccgtcaa cgtatccttg
     3961 gtagatttga gcttccaagc cgcgcgcgac accacagtcg aagaaatcaa cgcactgatg
     4021 aaagccgcct cggaagcagg cgcgctcaaa ggcgttttgg gctacaacac cctgccattg
     4081 gtttccatgg acttcaacca caccaccgaa gccagccact tcgacgcaac gctgaccaaa
     4141 gtcgtcgacg gcaacatggt caaagtgttc gcgtggtatg acaacgaatg gggcttcagc
     4201 tgccaaatgc tgaacaccgc acgccgtatg ttcggacttg aagtgcgccc gctcaaataa
     4261 gcgacaaacc gtcaaacaaa atgccgtctg aaacccgatg ttttcaagtt tcagacggca
     4321 cttttcattt ttgaccgcga ttttacccgg ctgtcgtcat ttccaatttt atagtggatt
     4381 aaatttaaac cggtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt
     4441 cgccgccttg tcctgatttt tgttaatcca ctataatcca ctatatttga acctgaccga
     4501 agcataagcc gccatcaaag ccccttgcac gccacttcgc gccgcccgat ttcctctttc
     4561 aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat ccgcaaacgg cagcagctcc
     4621 gaaccgaaac ggcgcatatg ttccgtgtcc tgctggcagt ctatcagttc cacgcccaaa
     4681 tccgccaaaa acggcacggc gcaggcaaac gcgattttcg acgcatccgg ttgcaacgca
     4741 aacatcgatt cgccataaaa caccctgccg atctgaacgc cgtaaaagcc gcccgccaac
     4801 ctcgttttac cgttttcatc gggataatgg cactcgaaag aatgcgcgtg tcccatttcg
     4861 tgcagcttca aatatgccgt ctgaaactcg ggcgcaatcc aagtcccatc ctgattcggg
     4921 cgcgccgctg ccgcacaatg cgcgacaact tccgcaaaac agccgttgac cgcaacccga
     4981 tagctgccgt tacgcagcgt tttcgccagc gagcgcggaa cgtgcagacg ttcgggaaca
     5041 attaccgcac gcggcccgac cgcataccaa aaaaaccaac cgtcccgcga aaaccaagga
     5101 aacacgccgt tccgatacgc ctcgagcagc cgccccgcgt ccaaatccct gctcacgccg
     5161 accagcccgt cgcaccgggc caaagcatag gcaggatcgg gaaaggcata attgtcaggg
     5221 gcaagcagcg gaatacgcat ggcagttctc caaaaacaca gacggcatcc aaaatcatga
     5281 atgccgtctg aacaacaaac cgtccggatt taacgtttgc ccttggcctg acagtcgctg
     5341 cacacgccgt acatataaag cgcgtgatcg acgatgcggt agccgttttc ctcggcgatt
     5401 ttgtcttgca gggcttcgat ttcgggattg tggaattccg ttacctcgcc gcacttcacg
     5461 cagacgatat ggtcgtggtg gtcgcccttg tccaactcat aaaccgcctt gcccgtttca
     5521 aaatggtggc gttgcagaat gcccgcctgc tcgaactgcg tcagcacgcg gtaaatcgtc
     5581 gccacaccga tttccacgcc ctcttccagc agaatgcggt acacatcttc cgcactcaaa
     5641 tgctcttccg cgtgcttctc gaacaaatcc aaaatcttca aacgcgggcc ggtaaccttc
     5701 agaccgctgt ctttcagttg cgcaatgttg ctgaattttt ccataatatt caatatccct
     5761 gtaaaataat agacgctata atacgcaatt tcagcctgct tgcccactat cacaccatag
     5821 cagttcgcaa tagcaaaacc gcaacggcgg acgggcagtc ggacacagac agggtaaaat
     5881 accgcttaag cgtatgatta tcgttcgctt ttataaaata ttcaagcagt gctacactag
     5941 acatcccgat ttgcacagaa aggttctccc gtgaacaaaa ccctcatcct cgccctttcc
     6001 gccctgttca gcctgaccgc gtgcagcgtc gaacgcgtct cgctgtttcc ctcctacaaa
     6061 ctcaaaatca tccaaggcaa cgaactcgaa ccgcgcgccg ttgccgccct gcgccccggc
     6121 atgaccaaag accaagtcct gctcctgctc ggcagcccca tactgcgcga cgctttccat
     6181 accgaccgct gggactatac cttcaacacc tcccgcaacg gcatcatcaa agaacgcagc
     6241 aacctgaccg tctattttga aaacggcgta ctcgtccgca ccgaaggcga cgccctccaa
     6301 aatgccgccg aagccctccg cgcgaaacaa aacgcagaca aacaataagg aacacacatg
     6361 acaccgctca aaatcgccat cgccggcgca aacggccgca tgggacgcgt cctcgtcgaa
     6421 gccgtcaaca accatcccga caccgtcctt tccggcgcgc tcgaacactc aggctcagaa
     6481 gccctcgggc tggacgcggg ctacgccgtc ggactcaaaa ccggcatcgc catttcagac
     6541 gacgttgacg ccgttctcgc ccaaagcgac gtactcatcg acttcacccg ccccgagccg
     6601 accctcaagc acctgcaaaa atgcgttgaa aaacaagtca acatcatcat cggcacaaca
     6661 ggcttcgacg atgcgggcaa agccgccatc cgcgccgccg ccgaaaaaac aggcatcgtt
     6721 ttcgccgcca acttcagcgt cggcgtcaac ctcaccttcc acatcctcga caccgtcgcc
     6781 cgcgtcctca acgaaggcta cgacatcgaa atcatcgaag gccaccaccg ccacaaagtc
     6841 gatgccccca gcggcacagc attacgaatg ggcgaagtca tcgccggcgc gctcggccgc
     6901 gacctcaaac aatgcgccgt ttacggccgc gaaggccaca ctggtccgcg cgatccttcc
     6961 accatcggct ttgccaccgt ccgcgcaggc gacatagtcg gcgaccacac cgccctcttc
     7021 gccaccgacg gcgaacgcgt ggaaatcacc cacaaagccg gcagccgcat gacctttgcc
     7081 gccggtgccg tccgcgccgc agtttgggta aacggcaaaa cgggtttgta cgatatgcag
     7141 gacgtactcg ggctgaacaa ccgttaaccc ccatacaaaa tgccgtctga aaagatattg
     7201 ttcagacggc attttgccga caggcttcgt atcggcatat caataaagtt gcttgtgcag
     7261 catgacaagc tacggctcgt ttaattgaaa ctctcctgat ctaaaaattc caactctatt
     7321 tcccggcaaa ctatatctat gataaaactg gtcggaagat tatacctacg ccttattaat
     7381 gccagtgcac ctccataatc caagatttca tcatcctgct ttaattctcc aagctccaag
     7441 gtattatcga tatctttatc ttggttctcc gtagcgtatc catagataaa tttcaaatag
     7501 ctgaactgct tgaatttaat ttttacccag tctgtgtaat ttggtgtagg acacaattgc
     7561 ctaaaataga aatttacgaa gaacaaatga aacaaggtac gtaagcgtat tagtataaac
     7621 tataatcata tataataaaa ttaatcagtt aacgaaccat tatattttaa ccttaatccc
     7681 attcgggcca gttctgtttc atttggaatt cctcttaaat acttattgtt tgatgtgcca
     7741 ttaatcacaa tttctccccc tttttttgta atacgtgctg cttcgggaaa ataatccatt
     7801 aaccgctcaa catcccgcct cttccaaaac cgcacgcgcg gccaaatccg ccgtatcttt
     7861 tttcaacagc aggtgcaacg ccccgaaatc ctgttgcagc gcggcaacct tatcggggtg
     7921 ttcgtaccag tccgccaatg ccgccgccag tttttccggt tttgcttcag attgcaataa
     7981 ttccggcacg gcctccttac ccaacaggat attcggcagg ccgacatgcg gcactttgat
     8041 tttgcgtttc acataagcat aggtcagcgg cgaaatcttg tagctgatga ccatcggacg
     8101 cttgcacaac gccacctcca aagtcgccgt accgctcgtt accagcaccg catccgccgc
     8161 cctgcacacc gtttcagact gtctgtcggt taccgtcagc gccaatccgg caaactccgg
     8221 ccgctgcaaa acttccgcca aacgccgctt cgtcgcctcc gttgcggcag gcagcaggaa
     8281 gcgggcggcg ggatagcgtt ccaacaacaa taatgccgtc tgaaaaaaca ccggcgccat
     8341 atagtcgatt tcgctgacgc ggctgccggg cagcagggcg aatacgggga tgccgacatc
     8401 cgcgcccaaa gttttccgcg ccgtttcacg gtcgtcttcc aagggcataa gctgtgccat
     8461 cggatgaccg acaaactccg cacgtccgcc cgcatcgaga taaagctgcg gctccatcgg
     8521 gaacaggcac agcacgcggt tgacctgatg cacgattttg cccacgcgtt cgcgccgcca
     8581 cgcccaaacc gacggactga cataatgcag ggtcggaatg cccgcccgtt tcagcttttc
     8641 cgccacaccc agattaaaat cgggcgcatc gataccgaca aagacatcag gtttcaacga
     8701 cagcaaatcc cgtaccagct ccctgcgtat ccgtaaaatt tccggcagcc gcctgaccac
     8761 ttcgacaaag ccgcgcaccg ccagccgctc ctgatcataa aggctctcga aaccttccgc
     8821 cttcatcagt tccccgccga tgccggttaa ccgtgcctgc gggcaacgct ttcggatggc
     8881 gcgtatcagg tgcgccccca acaggtcgcc cgacgcttcg ccgacactga cggcaatcaa
     8941 agggcttttt ttatcaacca tattcgtctg tccccacata tagtggatta acaaaaaccg
     9001 gtacggcgtt gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg tcgccttgtc
     9061 ctgatttttg ttaatccact atactttcac gccccgatgc cgtctgaagc cttcagacgg
     9121 catcggggtt cacattttcg tcccgttttc caacatcacc gcaaactgcg ccaaatccgg
     9181 cggcggctcc gccttcaaca ccagcggctc acccgtgagc ggatggttca ggtgcagctc
     9241 ggacgcgtgc aaaaacatgc gcttcaaacc caatttctgc aaacgccggt tcgcctgata
     9301 atcgccgtag cgttcgtcgc ccgcaatcgg acagccttga gattgcagat gcacgcggat
     9361 ttggtgcgta cggcccgttt tcagcgttgc ccgcaccaaa gtcaggtgcg acagcccgac
     9421 accgtgcaaa atgccgtctg agaaacggct taacacacgg aacaccgtat gcgccgactg
     9481 cccgtcctcg ctgacgcgta ccatcttttc gccttgtgcg ccgatatatt tgaacagggg
     9541 cagtttgaca tggaaattgt cgtccggcag tttgcccacc cccagcgcaa ggtagatttt
     9601 tttggggtgg tcgttacgga tagcttcgtg cagtttgacg agcgcgctgc gtttcttcgc
     9661 caccatcaac aagccgctcg tatccttgtc caaacggtgg accaactcca aatacttcgc
     9721 ctccggacgg gcgcggcgca actgttcgat aacgccgaaa ctcacgccgc tgccaccgtg
     9781 taccgccaca ccggaaggct tattgaccac cagtagcgcg tcgtcctcat acaccacctc
     9841 aaacgcacgc gccggtacgg cggcacgcct ttcagacggc atttccttct ccgccacgcg
     9901 cacaggcgga atccgcaccg tatctccctc cgcaatacgg ctgtcgggtt tgcagcgttt
     9961 cttgttcaac cgcacctcgc cggcgcggat aatgcggtgg atatagccct tgggcacgcc
    10021 cttgaggatt tttatcagat agttatcaag gcgttgaccc gcctcatgtt cggcaacccc
    10081 tatcaggctg accgaacctt tgcttatttc gtgcgttttc atctataatc cgaacatccg
    10141 tttcagcaga aagcgcgtcc gccgcgcccc ctgaaacggt ttactttaaa acggaatttt
    10201 atattaaaaa cgcactccgc aaagcatttt ccctgcgcct gttcccagcc ggcaggcgca
    10261 gaacgtagtc aagtttgaat tgattttgcc gtttcggcgc ggaagtaaga cggcagcgga
    10321 gccccaagcc ccgaacgcaa accgcccgaa aacacaggca tcacgataac aagaagaagc
    10381 cggcgcccat ttttccgacc gcagcgttcg gaacgcggca ggcagaccgc cgcatcgatc
    10441 tttatagcct tctttatccg acccttccgc acacggttcc ccggaacgtg catccctgcg
    10501 gattttcaac tcaaaaactg attaccggtt ttatccgaag acatcggaaa cccagcctta
    10561 cggaatgccg aaccggaccg gcgcgcccga atgccgcctg tccacgaggt gatcatgaaa
    10621 agaatgttat tcaacgcaac gcaggccgaa gagctgcgcg ttgccatcgt cgacggacaa
    10681 aacctgctgg atttggacat cgaaacgctg ggcaaagaac agcgcaaagg caatatctac
    10741 aaaggcatca ttacccgcat cgagccgtcg ctggaagcgt gtttcgtcga ttacggaacc
    10801 gaccgccacg gctttttgcc gtttaaagaa gtatcgcgtt cgtatttcct cggctacgaa
    10861 ggcgggcgcg cgcgcattca ggacgtgctc aaagaaggca tggaagtcat cgtccaagtc
    10921 gaaaaagacg agcgcggcaa caaaggcgcg gcgctgacca ccttcatcag cctcgccggg
    10981 cgctatctgg tattgatgcc gaacaatccg cgcggcggcg gcgtatcccg ccgtatcgaa
    11041 ggcgaagagc gtcaagaact caaagccgcg atggcgcaac tcgacattcc gaacggcatg
    11101 agcatcatcg cgcgtaccgc cggcatcggc cgcagcgcgg aagagttgga atgggatttg
    11161 aactacctca aacaactctg gcaggcaatc gaagaagcag gaaaagcgca tcacgacccc
    11221 tacctgctct ttatggaaag ctcgttgctc atccgcgcca tccgcgacta cttccgtccc
    11281 gacatcggcg aaattttggt ggacaatcaa gaagtttacg accaagttgc cgagttcatg
    11341 agctacgtca tgccgggcaa cgcaggtcgt ctgaaactct acgaagacca cacgccgctg
    11401 ttttcccgct tccaaatcga acaccagatc gaaagcgcgt tctcgcgcag cgtcagcctg
    11461 ccctccggcg gcgcgatcgt catcgaccac accgaagccc tcgtctccat cgacgtgaac
    11521 tccgcacgcg ccacacgcgg cgcggacatc gaagacaccg ccttcaaaac caatatggaa
    11581 gctgccgaag aagtcgcccg acaaatgcgc ctgcgcgact tgggcggctt ggtcgtcatc
    11641 gacttcatcg acatggaaaa ccccaaacac cagcgcgatg tggaaaacgt cctgcgcgac
    11701 gcactcaaaa aagaccgcgc ccgcgtacag atgggcaaac tctcccgttt cggacttttg
    11761 gaattgagcc gccaacgcct gaaacccgct ttgggcgaaa gcagccacgc cgcctgtccg
    11821 cgctgcgccg gcaccggcgt gatccgcggc atcgaatcca ccgccctgca cgttttgcgc
    11881 atggttcagg aagaagcgat gaaggacaac accggcgaag tgcgcgccca agtgcccgtc
    11941 gatgtcgcca ccttcctgct gaacgaaaaa cgcgccgagc tgtttgcaat ggaagagcgt
    12001 ttggatgtga acgtcgtcct gattccgaac atccacctcg aaaatccgca ctacgaaatc
    12061 aaccgcatcc gcaccgacga cgtagaagaa gacggcgaac cgagctacaa gcgcgttgcc
    12121 gagcctgaag aagacgaatc cgccaaacct ttcggcggcg aaaaagccaa
//
LOCUS       DAXFWM010000070        11930 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000070 DAXFWM010000000
VERSION     DAXFWM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.070"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..227)
                     /locus_tag="ACFI8B_001820"
     CDS             complement(<1..227)
                     /locus_tag="ACFI8B_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3834974.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVANHRLALAADEVFEDPAGPGGSCFGG"
     gene            599..826
                     /locus_tag="ACFI8B_001821"
                     /pseudo
     CDS             599..826
                     /locus_tag="ACFI8B_001821"
                     /inference="COORDINATES: protein motif:HMM:NF015188.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
     gene            1503..1961
                     /locus_tag="ACFI8B_001822"
     CDS             1503..1961
                     /locus_tag="ACFI8B_001822"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3834975.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2444..3856)
                     /gene="trkA"
                     /locus_tag="ACFI8B_001823"
     CDS             complement(2444..3856)
                     /gene="trkA"
                     /locus_tag="ACFI8B_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC3834976.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3954..5477)
                     /locus_tag="ACFI8B_001824"
                     /pseudo
     CDS             complement(3954..5477)
                     /locus_tag="ACFI8B_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5784..6590)
                     /locus_tag="ACFI8B_001825"
     CDS             complement(5784..6590)
                     /locus_tag="ACFI8B_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC3834977.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6642..6893)
                     /locus_tag="ACFI8B_001826"
     CDS             complement(6642..6893)
                     /locus_tag="ACFI8B_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834978.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6995..7765
                     /locus_tag="ACFI8B_001827"
     CDS             6995..7765
                     /locus_tag="ACFI8B_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834979.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7911..9080
                     /gene="metZ"
                     /locus_tag="ACFI8B_001828"
     CDS             7911..9080
                     /gene="metZ"
                     /locus_tag="ACFI8B_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC3834980.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            9175..9381
                     /locus_tag="ACFI8B_001829"
     CDS             9175..9381
                     /locus_tag="ACFI8B_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC3834981.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            9777..10001
                     /locus_tag="ACFI8B_001830"
     CDS             9777..10001
                     /locus_tag="ACFI8B_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834982.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            10030..11316
                     /locus_tag="ACFI8B_001831"
     CDS             10030..11316
                     /locus_tag="ACFI8B_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC3834983.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQLGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDREWQHIKDIEYEERQISDFLNN"
     gene            11330..11641
                     /locus_tag="ACFI8B_001832"
     CDS             11330..11641
                     /locus_tag="ACFI8B_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834984.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            11655..11858
                     /locus_tag="ACFI8B_001833"
     CDS             11655..11858
                     /locus_tag="ACFI8B_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3834985.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT         2948 a         2825 c         3342 g         2815 t
ORIGIN      
        1 ccgccgaaac agcttccgcc cggcccggcg gggtcctcga aaacctcatc ggcagccaag
       61 gccaaacgat ggttggcaac cgtgcggatt ttacggtaaa acagtgccgc cgaatcggga
      121 tggataccca aaatatcggc ggcagaacgg gcggtaactt ccggtacaaa agaacggagc
      181 ggttctttct gtacttcttt ctttaatttg cagtgtgtta tcttcatatt tcgagggtaa
      241 catatctgct aatctggtac agacccgaat gttaagcaga aacgattgtc tcaaaaaaag
      301 caactttgga taaatacaat caagaattgg ctgaactagg tgaaaatgaa aagaaaaaag
      361 aagaactaga aaaaaagtaa aacagcaaga aaacaaaatt cagatcagaa aaaggcacat
      421 agaacgtcta aaagaagaca tagaaaaggt tgcggtactc gaagaagaaa tcaaaagatc
      481 tgaaaagttc ttcgatgaaa acggttggct taaagacagc attatcgacg acaaaacaaa
      541 aaacgccgtc aacctcaaaa tgagcaacgg cgcgcgttgg acggttacca atgattcaat
      601 gctgaaagag ttggatttgt cggaagacgc gcaggtcgaa tttagcgaca acaataaatt
      661 cgtcaaagtg tccgtgagca agctcaaggg cgatggcggc gtgttcaaaa tgtatggcga
      721 catcgtcaaa ggagaatcgg acaagctgat tacccgaaaa ggcagcgaag ggacgcacat
      781 catcgaatat atggacgatg ccaaggcaaa aacgacggga agggaatatc tgaaactggt
      841 cgaaaacaag ggcaatcaag aagacaataa agcatcaaat aaagcatcat acaaattgaa
      901 tgtccgctgt acggaacagg gagggtggtg ttttgccttg ggtgagtcgg gtgcttccaa
      961 aaaggtcaat atttccacag acggtaagcg ggacttctac ctctacccgg ataccctgac
     1021 gccgggcgca tcgagcagcg tccttttcgg cgaggcgttg tatcagttga acgcggtttc
     1081 cgatgaaacg ctggtgcagc gtatgggcga aatccacgcc gacggaacgc cgcaggaaga
     1141 caacaacgtt tggatcaaac gcgtcggcgg aaaattctcc ggcagccgca gcgattaccg
     1201 cgtgggcggt tacggcaacc gctattgggg ctttgccggc ggcttcaaca ggacggggtt
     1261 cggcgataaa tggattcatt acaaagggct gatgctccgc cacctccaat cgtcctacgc
     1321 ttccgaagac tacgtcggca gcggcaaaat ctacggcagg gcggcaggtg tttattccgc
     1381 ctggctcaac cgggaaagca gggcttatta tgatttggtc gccaacatcg cccgatataa
     1441 gggcagctac gggctgacca attacgccgg caaacgggtc gaatcggacg aggcgcgcct
     1501 gaatgcctat atgctttccg ccgaagccgg taggcggatg gaaaaacaag acggcggcaa
     1561 aaacctatcg gtggcagccg gaagtgcagt tgtcttactg gtttacgcgc ggctacggct
     1621 ttccgctgtc aaacgggctg tctgccgaga cggacaactt ccgcagcctg atggggcggt
     1681 tcggctttcg ggcgggcgtg gacggtttgg acggcggcag gctgaacatc tacggcaagc
     1741 tgatgtacaa acgcgagttt atcggcacga tccgccacag gttcaacggc tctgccgtgg
     1801 aggaattcaa acaccggggc ggctggttgg aatacggctt gggcgtggtg cgccgcaatg
     1861 ccggaaacgg acggcagctt tattttgagg cgcaaaggtc ttcgatgcac acgatgcgcc
     1921 agaattggca ggtcaatatg ggcgtgcgca gtatgttctg atgcgcggat acgcctgaaa
     1981 acaatccgga acggtcagac gcgttccgga ttgttttttt gtcggggcgg tttgctgcca
     2041 tatcaaaaca ggcgggcgat gccgtctgaa acttcgtcat tcccgcgaaa gcgggaatct
     2101 agaacctcaa actttcagat aatctttgaa tattgctgtt gctcaatggt ttggattccc
     2161 gcaacctttc gtcattcccg tgaaaacggg aatctagaat ctctaaagct tcagctaacc
     2221 tttgaatatt gccgctttcc aatggtctag attcccgcct gcacgggaat gacgatttgg
     2281 aaattacccg aaacccaaaa acaactgaaa ccgaacaggc cggattcccg ctacgcggga
     2341 atgacggggg ccggatgccg tctgaagcgg tgggctgaag cccactctac tattattttt
     2401 gcttccatac cgcttggcgg caatgtgtcc ggaatggagc ggtttatccg aaaaagccca
     2461 ttttgacttg gatgagtttc tccagttcgt tcaggatgcg ccggcgcgag acgaaaaaga
     2521 tgatgtggtc accgtcttgg atgacggttt cggtatggtg tcccataatg gtttcgccgg
     2581 ttccggcgcg gacgacggcg gcaatgtggc agccttcggg ccatttgatg ccgctgatgc
     2641 gcctgccgat gatggcggaa gtttttttgt cgccgtgcgc gacgacttcg atggcttccg
     2701 ccgtgccgcg ccggatgggg tggacggcaa cgatgtcgcc gcgccggatg tgggcgagta
     2761 tcgagccgat ggtgatgagg tggggggaga cgacgatgtc gattttgttg ccttcgagca
     2821 aatcgacgta gcttgagcgg ttgacgatgc cgatgacgcg cttcgcgccg aggtttttcg
     2881 ccaaaagggc ggacataatg ttgctttcgt cgtcgttggt cagggcgcag aatacgtcga
     2941 tttcgtcgat gtattcgttg tcgagcaggg tttcgtcggt tgccgaacct tgcaggacga
     3001 gggtgttgtc gaggttttcg gctatccatt cggcacggcg cggccggcat tcgatgattt
     3061 tgacgttgta tgcgtgttcg agctgcttgg cgaggcggta gccgatgttg ccgccgccgg
     3121 caatcatgat gcggcgggtg ctggtttctt tggggcgcaa ttcgggtatg accgccccga
     3181 tgttttcggc ggcggcggca aacaggattt cgtcgccttc gatgatgacg gtttgcggcg
     3241 cggggacgat gaggcggttg ttgcggtaaa cggcgcagat ttggcagtcg gccccgtcgg
     3301 gcaaatcttg ggcgatgtcg gcaatgctgc gtccgacaag cagcccgccg cgccgcgcct
     3361 gtatgatgac catccgcacg cggtcgtctg caaaacgtaa aacctgcaat gcgcccgggc
     3421 agtctatcag gccggcaagc tgttcggtaa cgagctgttc ggggctgatg gtttcggtta
     3481 tgccgaatat ggaaaggctg ccgttttcgt tgttttcgag cttggggctg aggtattcga
     3541 ggtattcgct ggaacggacg cgcgcgatgc ggccggggat gttgaacagg tcggcggcaa
     3601 ctttgcaggc gacgatgttg gtttcgtcgc tgcgggagag cgcgagcagc aagtcggcat
     3661 cttccgcgcc ggcgcgttct aatgtgaagg gggatgcgcc gttgccgaaa acggtttgga
     3721 catcgaggcg gctgcctgtt tcctgcaatg ctttttcgtc gatgtcgata acggttacgt
     3781 cgttgttggt gatggcggca aggttttgcg cgacggtaga acctacctgc ccgttgccta
     3841 aaatgaggat tttcacggta tgggtcgccg ggtgatgaaa tggtggattg taccgcaacc
     3901 ggcggcggaa atgccgtctg aaggcagatt ttgcgttcag acggcattgc gcttcaagat
     3961 tcgacgggga tgatgccgat tttcgcctgc catttgcgcg gggcggtggc gtggatggat
     4021 tcgcctttgc tgtccacggc gacggttacg ggcatatctt tgacttcaaa ttcgtaaacg
     4081 gcttccatac ccaattcggg gaacgccaag actttggaag atttgatggc ttttgccacg
     4141 aggtatgccg cgccgccgac tgccatgagg tacacggcct tgttgtcggc gatggcttcg
     4201 caggtggccg cgccgcgctc ggatttgccg atcatgccca agaggccggt ttgtttgagc
     4261 atttggcggg taaatttgtc catgcgggtg gctgtggtcg gacctgcggg accgacgact
     4321 tcatcgccga ccggatcgac ggggccgacg tagtaaatca ggcggttggt gaaatcgacg
     4381 ggcaactcct cgcctttgtc gagcatattg acgaggcgtt tgtgcgcggc atcgcggccg
     4441 gtgaggattt tgccgttcaa cagcaatacg tcgccggttt tccagcttgc cacttcttct
     4501 ttggtcagct tatcgacatc gacgcgtttg ccgttgtcgg ggctgtaagt cagatcgggt
     4561 cagtcttcga cgcgcggcgg cgtgagttcg acaggacctg agccgtccaa ttcaaattcg
     4621 acgtggcggg tggcggcaca gttgggaatc atggcaatcg gtttggaggc ggcatgggtc
     4681 gggtaatcga ggattttcac gtccaacacg gtggtcagac cgcccaagcc ttgcgcgccg
     4741 atgcccagcg cgttgacctt ttcaaagagt tcgaggcgca gggcttcggt ggtggacaat
     4801 tccgcgccgg acgcggcttt ttcctgcaat tcttggatgt cgatgtggct catcagggat
     4861 tctttcgcca tcaacacggc tttttcgggc gtgccgccga tgccgatgcc caagatgccg
     4921 ggaggacacc agcccgcgcc catcgtcggg atggttttca atacccaatc gacgatgttg
     4981 tcggaagggt tgagcatagc gagtttggat ttgttttcag agccgccgcc ttttgccgcg
     5041 caggttactt cgactttacc gcccggcacg atgctcatgt ggatgacggc gggggtgttg
     5101 tctttggtgt tttggcgttt gccggccgga tcggcgagga cggaagcgcg cagggtgttg
     5161 ccttcccaag tgtaggcgcg gcgtacgcct tcgttaacca tctcttccac gctcatgtcc
     5221 gcatcccatt gcacatccat accgactttg aggaagacgg ttgcgatacc tgtgtcttgg
     5281 cagatggggc ggttgttttc ggcacacata cggctgttga ccaaaatctg cgtcatcgcg
     5341 tctttggcgg cgggattttc ttccttctgc cacgccttat aaagcgcgtc gatgtagtct
     5401 tttggatggt agtagctgat gaattggaag gcatcgcaga tactttgaat aaagtcttct
     5461 tgcttgataa cggtcatgat tgtgttcctt ttcaaggttg tggcggtaag tgtctgctgc
     5521 tgttttaaac aacctttttg aaggctgttt tttatcaggt tgaaatagtg ccagcgtttg
     5581 tacggtgaag acaatacata attctaatct cttgattatc aatttgtttt aataaaatac
     5641 tgttttattc ggattttgat atttgtaatc tttgcctgtt gctttgcggt agggagaaat
     5701 cagcaggcag ttcattgtgg accggttgat gaacaatgcc gtctgaaagg gcttcagacg
     5761 gcattttgtt cggacgggcg ggcttatttc gcagcctgtc cgccctcttt ggcaaaatat
     5821 ttggcgtaga tcttgtcgta ttcgccgctt tcgcgtactt tttccaacgc atcgttcagc
     5881 attttgacgg ttgcttcgtc gcctttgcgt accgcgatgc cgtagtgttc ggtggtgaag
     5941 tcgggcaggg taacgaagtc cattcctttg gccgggttgt ttttcacata attggcgatg
     6001 accgcgctgt cgctgaccac ggaatccaag ccgccgtttt ccagttcttt gataatcagg
     6061 gggacgtttt caaagcgcgc gattttcgga ttgtcgttgc ccaagagttt ggaaacggag
     6121 aaatcgcccg tgtggccggt aaccacgccg actttgttca tctttttcaa atcttcggaa
     6181 gaagatactt ttttgccttt cggaacgagg acgacttggg tgatttcaaa atacgggtcg
     6241 ctgaaatcca tagactgttt gcggtcgtcg gtaatggtta cgcccgacat cacaacgtcc
     6301 gcatcgccgt tgttcaaggc ggggaaaagg ctgtcccacg gctggtgttt gaattcgatt
     6361 ttaaaattgc ccgccttcgc catcgcgttc atcaaatcca cgtcgaaacc ttcgacattg
     6421 cctttcgagt ctaaagattc aaagggggca aactcggcgt tggaagccac gcggtacact
     6481 ttgccggggt tggcggcagg cgcggcggca tctttaccct gaccgccgca ggcagacagc
     6541 gcgagtgcgg aacaggcaag ggcggcggca atccattttt tcatattcat caaaattcct
     6601 ttcggtaatc aaaaagccta aaaaatcagt ttaacataaa attaacgttc cttttcacgg
     6661 aaaaacgcat aagtaacgtc gtggaacaaa tccgtatgcg ggctgtcctc atagctcaat
     6721 tctgccaagg cgtcctcaaa agcaagctga atcgactcga ccgcgatttc ggcggtgtct
     6781 gcgggaaggg cgcggatcag ggctttgaag gcggtggaca ggacgtggtt ctgcatacgc
     6841 agcacgtcgt ttgcctcttc cagatattcc aaacgctgtt cgatgctgcc catgatgtat
     6901 cctcgcgaat ctgttaaaat gcgccattat atcaaactgc gctttgggcg cgggtttgcc
     6961 cgcctgcaaa gattaagaac cggaagccga aaaaatggat acaaacaatc aggataaaga
     7021 gccgaattgg gaaaaacgcc tggaaaacct gcgtcagacg cgccgtatga ataccgcgcc
     7081 tgcctatgtg ccgctgacca aaccttctga agtcggaaag gaagagccca taccgaaatt
     7141 ggacgatgca aaccgagacc gtgtgttaaa ggcgtatctg aagaaatggc aggaagaaca
     7201 caacagcgcc gttgccgatt gcggcgagga agtcgaaacc gacccgatga tcgtccttca
     7261 ggaaaactgg ctcaacgcgc aggcttccct gcaaatgccg gtttcggaaa aacacatcga
     7321 atcgcggcgg agaatccggt tcgatgccaa aaaagacagt gccgaatttg aaaccgcaca
     7381 gattgagggc ggggaaaacg ccgatgcggc agaatccggt acggcggcag acggggttgc
     7441 ggaaaacggc gaaggcggtg cggaagaggc gttgatgccc gtccaaatca atatcctgaa
     7501 cccgaaagcc gtcaaccggc gcgaagtgtt ctgcctgtcc gaacaggagc tgaccgaacg
     7561 gttgatcaaa cgcctgcgcc cgcatctgac cgataccgtc aacggcatga tccgtacggc
     7621 ggtgcagaag cagatggcgc tctttactta tcagctccag caaatgctgc acgagcaggc
     7681 gggtgcggta gtcgaagacg tgttggaaca cgatgtccgt aaaatcctca acgacatcaa
     7741 gtacgaatta aaatacaaac gctgattgcc atgccgtctg aaagccgtgt tcagacggca
     7801 ttttcctgcc cgtccgactt gaaagcctca tggaacaggc gcaaaatatt aataaattaa
     7861 acaacgggtg cacgccccgt tgccgaacgg acaaaaccga ggaatcaccg atgagcaaaa
     7921 aacttcatcc gcaaacgctc gccatacgcg gcggcaaaga acaaaccgga taccgcgaac
     7981 acaatcaggc attgttcctg accagcagct tcatgtggga caacgcccaa cacgctgccg
     8041 atttgttttc aaaaaaaatc aaaggcttca cttatacccg taccgccaac ccgaccatcg
     8101 cggcctttga aaaacgcata gccgctttgg aaggcgcgga acgcgcggtc gccacttcga
     8161 ccggcatgtc cgcgattcag gcggcgtttt tcaccttcct gcaggcgggc ggccatgtgg
     8221 tttccagccg cagcctgttc ggcacgaccg tcggctttat caataacatc gttaccaaat
     8281 tcggcatcgg agtgagccgc gtgtcgccga ctgatataaa tgaatggaaa gccgccgtca
     8341 aagccaacac caaactgctg tttttggaaa cgccgtccaa ccccttgggc gaagtggccg
     8401 acttggaagc cttggcggaa ttggcgcacg gcatcggcgc gctcttggtg gtggacaaca
     8461 gcctgctgtc gcccgtcggc tcgcagcctt tgaaacacgg cgcggatatt tccgtttcct
     8521 ccgccaccaa agccatagac ggacacgggc gcgtgatggg cggcgtgttg gcgggttcgg
     8581 aagaactgat ggcgcaggtc gccgtttact gcaactcttg cggtttggcg atgtcgccgt
     8641 ttaacgcgtg gcagttgttg agcggcgtgg aaaccctgtc gctgcgtatg gaaaaacagt
     8701 ttgacaacgc cttgagaatc gcgcaatggc tgcaagagca gccgcaggtt caagcagtgt
     8761 actacaccgg attgcccgac catccgcagg cggagcttat ccgcaaacag caaaacggcg
     8821 gcggcatcgt catcggtttt gaagtcgccg accaagcagc cgcgtggaaa gtcgtggacg
     8881 gcgtggaact cttttcccgt accgccaacc tcggcgacgt gcgctcgacc atcaccaatc
     8941 cgtggacaac cacgcacggc aggatgcagc ccgaagaaaa actcgccgcc ggtatccgcc
     9001 ccggactggt gcgcctgtcg gtcggtttgg aatatgttgg cgatttgatt gacgatttga
     9061 aacaggcact cgcgcgctga ttgcgtgttt cagacggcat cggcggaaaa atgcggcaga
     9121 tgccgtctga aacccttatt gcgtatggca tacacatcaa caataggaaa aaatatggat
     9181 gaagcggttt ttgccgattg ggcgttgaaa atctgcctga ccggtttgat tatttttttg
     9241 ggttttatcg tttggaattt gggcaaggag tccaaagcgg gcaaattcgg catcgccgtt
     9301 ttgtttttgg ttttgggctt gggtgtgttc ggttttgtgt tcaaagagtt attgattaag
     9361 tttttggtat tgcccaaata ggtttgccgt ctgtcgaaat gccgtctgaa atcataaaaa
     9421 gtaatctgca ccctaaaagt ttgatttaac cgccaactga ttaaggtgcg ggtttttatg
     9481 acaaaataaa aatcatcttt ccgcaatcat tcccacggat gcgaaagcct gtctttttga
     9541 atcccggata attttacatc gtcattcccg cgcaggcggg aatctagtcc gttcggtttc
     9601 agtcatttac gataaattcc tgttgccttt catttccgga ttcccacttt cgcgggaatg
     9661 acggaggagc ggataaattc tcgcaatcta aaaccttata tattgcgctc cataaggggc
     9721 gcatattttt aatccttgta aagcccctta actctagtca gtgaaggggc ttaatcatgt
     9781 ttattttcta tactgtcaat cccgaacacg tatatttccc caaagcatac atcatgaaag
     9841 tgttcaagga taaaggctac gaatcccaat gcatcacaac agtttctttt tatatctgca
     9901 atccgacttt gaagcaaaag accgaaaacg aagcgtatga atacggcaga ttgtttgtta
     9961 aagaactgat gcataaggaa tgcaaccgtg aaagcctttg aagataaagc cttggcggtg
    10021 tccgcggaca tggcggacgc gttagcggac ggcggggctg cggcaaccgc cgtaggctgc
    10081 ccccctaggc taataggggg ggagcaaaat aaaaccccaa atccgaaggg tgcggaaaaa
    10141 acagaaaacc aagaatttca attcgagtat ttcagtcatt ttgtatcgga cggcaaaggc
    10201 agatttatag aaataccgtt aaggcgtggc agggatgacg gagcgttcat agaccaaatc
    10261 actttcacga tacatgaaaa ttccatgacg aaagtaaccg gaaagggatt ggtatcagat
    10321 acggaattcg ttgtccggta tagcgagctg cttgaagaaa tcttgggatt cggcattacc
    10381 aaaaaattac cgtttaaagg aaagtttttc tatcaaagct gttatcaatt cgggcccgat
    10441 aacgtcgaat acggaaaggt tcattacggc ggccaatgtg aaacgatgtt ggttgaattg
    10501 aacggtacag gctgcatggc tgctttaccc ggttgggaaa accgattgta tgaattttta
    10561 agtaaatgcg tccgacccaa aataacccgt atcgatgtcg cccatgattt tttcaacgga
    10621 gagtacacac ccgatcaggc aatgttagac cacgataacg gacattacga cgtacacaat
    10681 atgcggccaa aaagcgaatg tcgcggcacg gcatggcgca atgaagacgg cagtggaaaa
    10741 accttttaca tcggcaagcg cggaaattca aaatttaccc gtgtttacga gaaaggcaga
    10801 cagcttggag atgttgatag cccttgggtc aggtttgagg gggatataga aatacccttg
    10861 gatgtcctac tttattcggg gtcgtatcta ggcggcgcat atccgatttg caaggagatt
    10921 tttaagacag aagccaagcg catggaagta aaagttaaaa acgttaatct gatattcgac
    10981 gttaagcttt ttcatgcaag gaatcaagtc ggcaagatgg ttaatttcct ccgcgatata
    11041 gggtgggacg atagcaggat agttgatgag ctggtgaaag gcgttgaagg ttatccaaaa
    11101 ggtttacagc ctgagcaata cgattgcaaa aaccaaacgc agaaattcca gtatatccac
    11161 gaagaacaga aagccattaa cgcattgaac attgaaacac tgtttgatga tttgattgaa
    11221 gagagagaat acgcatttcc tcaagacagg gaatggcaac acatcaagga tattgagtac
    11281 gaggaacgtc aaatatccga ttttttaaac aactgaagga aattgaaaaa tgtttgaaac
    11341 aagccaagta accacatata ccgccacttt gttgggcgca aaaaaattca aaggcgaaat
    11401 cgacggtaac aaaatcgatt catgcaccgt cttggtagcc agccccatgc cgtcaaacgg
    11461 caatgccgtg ggctttacct cggaaagcat gaagtttggt gacagccata attttgaaaa
    11521 attgaaaaac cttaaattcc cctgtgcggt tgatctaacc gttgccatgg aatcaacagg
    11581 aaaaggctta gtgcagaaat tgttggattt ccaagtaaag ggcgcagcac ctaaagccta
    11641 agaaaggctg aatcatgagc agataccaac agaaatttat cgtacaggaa ttggaaaatc
    11701 acgaattcat ctatcccgat tcgtttggcg atattggatt tacgtcgaac atcaaatcag
    11761 ccggtaaata tgacagctat gaagacgcat tcagctcggc attggaagag ataggcgggg
    11821 aatttgtaat tttcggattc tacgaaaaag aagattaagt ttaagaggct cggcaggcgg
    11881 tctctaaaac cttcacaaag cccgcaaaca cattttttta aaaatttcgt
//
LOCUS       DAXFWM010000071        11766 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000071 DAXFWM010000000
VERSION     DAXFWM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.071"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(81..497)
                     /gene="dksA"
                     /locus_tag="ACFI8B_001834"
     CDS             complement(81..497)
                     /gene="dksA"
                     /locus_tag="ACFI8B_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC3834986.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(645..1454)
                     /gene="proC"
                     /locus_tag="ACFI8B_001835"
     CDS             complement(645..1454)
                     /gene="proC"
                     /locus_tag="ACFI8B_001835"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC3834987.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLEKELGVETSATLPELHSGDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1485..1892)
                     /locus_tag="ACFI8B_001836"
     CDS             complement(1485..1892)
                     /locus_tag="ACFI8B_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC3834988.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1892..2578)
                     /locus_tag="ACFI8B_001837"
     CDS             complement(1892..2578)
                     /locus_tag="ACFI8B_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC3834989.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2698..3741
                     /gene="pilT"
                     /locus_tag="ACFI8B_001838"
     CDS             2698..3741
                     /gene="pilT"
                     /locus_tag="ACFI8B_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC3834990.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3905..5131
                     /locus_tag="ACFI8B_001839"
                     /pseudo
     CDS             3905..5131
                     /locus_tag="ACFI8B_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
     gene            5248..7398
                     /gene="yccS"
                     /locus_tag="ACFI8B_001840"
     CDS             5248..7398
                     /gene="yccS"
                     /locus_tag="ACFI8B_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC3834991.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIRRLLEMQ
                     GQACRNTAQAIRSGKDYTYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
     gene            complement(7848..8132)
                     /locus_tag="ACFI8B_001841"
     CDS             complement(7848..8132)
                     /locus_tag="ACFI8B_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC3834992.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIDQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
     gene            complement(8784..>11766)
                     /gene="pilC"
                     /locus_tag="ACFI8B_001842"
     CDS             complement(8784..>11766)
                     /gene="pilC"
                     /locus_tag="ACFI8B_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC3834993.1"
                     /translation="TYKYAIVMNAQNQPEVRRNGQYSTIKGKDREREYTYHNTRGGGG
                     SVSFNNTDTLVSQQSGTAVFGTATYLPPYGKVSGFDADALKERNNAVDWIRTTRIALA
                     GYSYADVICKSGNQCPQLVYETKFAFGQQGLQRKGNKLDIYEDKSRDNSPIYKLKDYP
                     WLGVSFNLGSENTVKNSKSFNKLISSFNEDNNNQTIVSTTRDHPISLGNWQREHTAVA
                     YYLNAKLHLLDKKRIEDIAQGKTVDLGTLRPRVETTGRGWLSFWATWDIKDTGQIPVK
                     LGLQVKAGRCINKPNPNNNTKAPSPALTAPALWFGPGQDGKVQMYSASVSTYPDSSSS
                     KIFLQNLSRNDDKNKPGRYSLKPLSENEIKSKEPRFNGRQTVIRLDSGVQLIKLNRSK
                     DEVVIFGNTGNNGTFGIVKEANVNLEADEWKKVLLPWTVRGPDNDNKFKLINQKPDKY
                     SQRYRIRENGNRDLGDIVNSPITAVGGYLATAANDGMVHIFKKTGTDERGYELKLSYI
                     PGTMPRQYFDNDTSALKDSTLAQELRTFAEKGYVGDRYGVDGGFVLRQVELSGQKHVF
                     MFGAMGLGGRGAYALDLSKINENYPAAAPLFDVKNGDNNGKNRVEVELGYTVGTPQIG
                     KIRNGKYAAFLASGYAAKDIDSQENKTALYVYDLKDTLGTPITKIDVPGGKGGLSSPT
                     LVDKDLDGIVDIAYAGDRGGNMYRFDLSNSDPSKWSVSTIFEGGKPITSAPAVSRLAD
                     KRVVIFGTGSDLSEEDVVGTNPQYIYGIFDDDKGTVKVTVQNGTGGGLLEQVLSEENK
                     TLFLNKRSDGSNGKGWVVRLREGERVTVKPTVVLRTAFVTIRKYTDTDKCGAQTAILG
                     INTADGGALTPRSARPIVPEANKDVAQYSGHKTAGGKSVPIGCMWKNSKTVCPNGYVY
                     DKPVNVRYLDETETDGFSTTADGDAGGSGIDPAGRRPGKNNRCFSKKGVRTLLMNDLD
                     SLDITGPMCGIKRLSWREVFF"
BASE COUNT         2752 a         3636 c         2664 g         2714 t
ORIGIN      
        1 ctcttctcct ccggctgatg cgcctttgtg aatatgccgc ctgaaactcg gggcttcaga
       61 cggcattttg tttgcctcca tcagtcggca aactgtttct tcatcctctc tcggcgttct
      121 tgggcctcaa cggataaagt ggctgtcggg cgtgccagca gccgcttcag accgataggt
      181 tctcccgtat cggcacagaa tccatagtcc ccttcatcaa tattgcggat ggtcgcctgt
      241 attttactga gaagttttcg ttcccgatcc cgggtacgga gttccaatgc gtactcttct
      301 tcctgtgtgg cacggtcggc aggatcgggg gctgattcgt gttcttggag atgccctgtt
      361 gtaacggagg cgttttcgat gagttcgtct tgcattttta ccagcaattc gcggaaaaaa
      421 gccaaatggt cgtcattcat ataatcgtct tccggcccaa tccaattcaa aatatcttgt
      481 tctgtcagct ttaccataat gtatgccctc tcggttcacc gcaaaaaaac aaagcactac
      541 aaagtggctt tattcctttg atttttataa ggcttattgc ctaaggcgtt tttgaaaacg
      601 cgcatcataa cacgttttgg attggttttt ttattttctt tacattattg atattgccgt
      661 tccatttcct gcgaacggca cacacaggca caaacgccct cacttatggc ttcggcgaca
      721 cgatgccgtc tgaaggcttc cacggcttcg tgggttgtcc cgccttttga cgttacattt
      781 ttttgaagct tctcgaaatc ttcacccgtc tgctcggcaa gggcaaccgc tcctttaaac
      841 gttgccagac tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat ggcggcattt
      901 tgcaatgcgt ccagcagata aaacacataa gccggcccgc tgccgctgat gccggtaatg
      961 ctgtgcattt gcgcttcatc ttccaaccaa acggtcaaac cgactgattt catgattcga
     1021 tcggcaatcc tgcggtctgt ttccgatact tccgcttcgg catacatacc ggatacgccc
     1081 agcccgattt ttccaggtgt attaggcata acccggacaa tgcggcggct tcccccgagg
     1141 taacggctga gcgtaccgac cgacaatccg gctgcgacag aaagcaccaa tgcgccgttg
     1201 gtgcggatat ttttgcacgc ggcttccata tcctgcggtt tgacggcaag aatcaaaaca
     1261 tcacccgaat gcaattccgg cagggttgcc gaagtttcga cccccaactc tttttccaaa
     1321 cgttcgcgct tttccgcacc acggttggct atatggatgc ggtatccccc ttgtttgacc
     1381 aatccgcccg caacggcagc cgccatattt ccgccgccga gaaaataaac attcattatt
     1441 tctaccctat tcaaaattac cttactaaat tcattcccaa ttttttacca tcggacgcgc
     1501 tcggacaggc cgttgcgccg caacccctgc ttttccaaac agtcgaaacc ttcctgtttg
     1561 aacctctcaa ccaaaggttt gccgtcacgc gtacgccaat ccaacccgca ggcttcaaac
     1621 ttccctattt tcctataaaa gtaagatttt tgcgcggatt catattcgtt tgccctgacg
     1681 gaacgattgc cgtctgaaag cggtatctcg tagtcctcta aagacagcgg actctcgccg
     1741 ccaatatccc aaaaatcgat ggcggcaggt tttctcggct tacaccagcc ggacaaggac
     1801 gaacactcat accaccccgc cagccggcag cctgcaaccg taaatgcaaa caacgcagcc
     1861 gttgccgtat attttattac ccgaatttcc atcagcccct tttcccgaaa atcgcgctgc
     1921 cgatacggac gtgtgtcgca ccgcactcaa tggcggcagg catatcgtcc gacatcccca
     1981 tagacagcac gtccgcctta acgccagccg cattgaggtc ggcaagcagc ttttgcatgg
     2041 tctgaaactg agttttcaac tccgtttcac tgctgttggc tttggcaacg cacatcagtc
     2101 cgcgcacgac aaggttgggc agcttcgcca cttccacggc aagcgcgacc gcttcttcgg
     2161 gcgcgacacc gtgttttgcc gcctcgcccg caatgttcac ctcgacacac acctgcaagg
     2221 gcggcattaa ggagcgacgc tgcctgctca gccggacggc ggtcttcaga cggcatacgg
     2281 tatgcaccca atgcgcgcgt tcggcgacaa atttggtttt gttggactgc acatcgccga
     2341 tgacgtgcca cacgatgtcg gtcaaatccg ccaattcctc cgttttgccg taccactcct
     2401 gaatatagtt ctcgccgaaa tcacgctgtc cggcagcgta aacttcgcgg atgccgtctg
     2461 aagggaaggt cttgctgacg gcaatcaggc tgacggaatg cggctccctg cccgcctgca
     2521 gaaccaattt tccgatacgg tcggacacct cacgataacg ttcttgcaac accgtcatag
     2581 atcatcccct aattaaaaat gattaaacag ttgaaacccc tccagtcagg gcggtataat
     2641 caaggttgtt agaatcattc caaccaatcg aaacattata ctaaacagag ccgcattatg
     2701 cagattaccg acttactcgc cttcggcgct aaaaacaaag catccgacct tcacctgagt
     2761 tcgggcatat cccctatgat tcgggttcac ggcgacatgc ggcgcatcaa ccttcccgaa
     2821 atgagcgcgg aagaggtcgg caatatggta acttcggtga tgaacgacca ccagcggaaa
     2881 atctaccagc aaaacttgga agtcgacttc tcgttcgaac tgcccaacgt cgcccgattc
     2941 cgcgtcaacg ccttcaacac cggccgcggc cccgccgccg tattccgcac cattcccagc
     3001 accgtcttat cgctggaaga attgaaagcc ccgagcattt tccaaaaaat cgcagaatcg
     3061 ccgcgcggca tggtattggt taccggccct accggttcgg gcaaatcgac cacgcttgcc
     3121 gcgatgatca actacatcaa cgaaacccag ccggcacaca tcctgaccat cgaagacccg
     3181 atcgaattcg tccaccaaag caaaaaatcc ctgattaacc aacgcgagct gcaccagcac
     3241 accctcagct tcgccaacgc gctgagttcc gcattgcgcg aagacccaga cgttatcctt
     3301 gtcggcgaga tgcgcgaccc cgaaaccatc ggcttggcac tgaccgccgc cgaaaccgga
     3361 cacttggttt tcggcacgct gcacacgacc ggcgcggcaa aaaccgtcga ccgtatcgtg
     3421 gacgtattcc ccgccggaga gaaagaaatg gtgcgttcca tgctgtccga atcgctgacc
     3481 gccgtcatct cccaaaacct gctgaaaacg cacgacggca acggccgtgt cgcctcgcac
     3541 gaaatcctga ttgccaaccc cgccgtccgc aacctcatcc gcgaaaacaa aatcacgcag
     3601 attaactccg tcctgcaaac cgggcaggcg agcggtatgc agacgatgga ccaatcgctg
     3661 caatcgctgg tgcgccaagg gctgatcgca ccggaagccg cacgcagacg cgcgcaaaac
     3721 agcgaaagta tgagtttctg acacacaacc gccttccggc catgccggcg ggaaaacaag
     3781 gcgcaaacac gcggggcggg acgcagcatc ccgcccggca tacccttccg aacaggaagc
     3841 gtccgccttc ctgttgaaac ctgccgccgc aaactgcaag gcttaaaccg aaaagaagct
     3901 aacgatgaat accgataacc tgcacgacat cttggacgaa acggttcaag tgtattctca
     3961 aaaaaaacaa agccgatccg aaaccccggc cgaaatcggc acacacttcc acccgctgct
     4021 cgaccgcctg tgcgaaaccg cagaagcaca aaacgcgtcc gacatcctta tcagcaaagg
     4081 attcccgccc tcgttgaaaa tcaacagcgc attaaccccg cagccgcaaa aggctctgac
     4141 gggcgaggaa accgccgcca tcgccgcatc gacgatgaac gccgaacaat cggaaatatt
     4201 ccggcgcgac ggcgaaatca actactccgt ccaatcgcgc agcggcacgc gctaccgcgc
     4261 caacgcctac cacagccaag gcagcgcagg tttggttttg cggcgcatca accacgtcat
     4321 cccgtaaatg cgggaattgg gcttgcccga aaaactcaaa gacctcgccg tcgcaccgcg
     4381 cgggctgctg attatcgtcg ggcctaccgg ttcgggcaaa tccaccacga tggcgactat
     4441 gctcgaacac cgcaacaaaa ccctgcccgg ccatatcgtt accatcgaag acccgattga
     4501 gtttatctac aaaccgcgcc gctgcatctt tacccagcgc gaaatcggcg tcgacaccat
     4561 aaactggcag acggcggtac aaaacgctat gcgccaatcc ccagacgtgg tctgtatcgg
     4621 cgaagtccgc agcagggaaa gtatggaata cgcgatgcag ctcgcccaaa ccggccacct
     4681 gtgcattttt acgctccacg ccaacaccgc gccgcagtcg ctcgaacgca tactcaactt
     4741 ctaccccaaa gaacagcaca accaaatact gattgacatc gccctcaatc tgaccggcat
     4801 catctgccaa cgcctcgccc tcaaaaaaga caaaacgggc aggacggcgg ttgtcgactt
     4861 gctcatcaac acgcccgcca tccaagactt catcctgaag ggcgacctga tgaacatcag
     4921 taaaatcatg gaaaccgcca aaaccgacgg aatgcagacg atggaccaaa accttttcga
     4981 actgtaccgt cacggcatca tcagttacga agaagccctg cgccagtccg tttccgccaa
     5041 caacctgcga ttgcacatcc aactgcacaa agaaggcaaa acgcccgaac tcctttacga
     5101 cagggtcaac ggtctcaacc tcatttcctg atccgcaaaa cccaatgccg tctgaaaacc
     5161 gcatccccgt tttcagacgg catgatttta tccgccccat tcatgtgcca cactttattc
     5221 acttcataac aataaaccgg taaaaccatg aaaaccccac tcctcaagcc tctgctcatt
     5281 acctcgcttc ccgttttcgc cagtgtcttt accgccgcct ccatcgtctg gcagctaggc
     5341 gaacccaagc tcgccatgcc cttcgtactc ggcatcatcg ccggcggcct ggtcgatttg
     5401 gacaaccgcc tgaccggacg gctgaaaaac atcatcgcca ccgtcgccct gtttaccctc
     5461 tcctcgctca cggcgcaaag caccctcggc acagggctgc ccttcatcct cgccatgacc
     5521 ctgatgacct tcggctttac cattttaggc gcggtcgggc tgaaataccg caccttcgcc
     5581 ttcggcgcac tcgccgtcgc cacctacacc acacttacct acacccccga aacctactgg
     5641 ctgaccaacc ccttcatgat tttatgcggc accgtactgt acagcaccgc catcatcctg
     5701 ttccaaatca tcctgcccca ccgccccgtc caagaaagcg tcgccaacgc ctacgaagca
     5761 ctcggcggct acctcgaagc caaagccgac ttcttcgacc ccgatgaggc agcctggata
     5821 ggcaaccgcc acatcgacct cgccatgagc aacaccggcg tcatcaccgc cttcaaccaa
     5881 tgccgttccg ccctgtttta ccgtttgcgc ggcaaacacc gccacccgcg caccgccaaa
     5941 atgctgcgct actacttcgc cgcccaagac atccacgaac gcatcagctc cgcccacgtc
     6001 gactaccaag agatgtccga aaaattcaaa aacaccgaca tcatcttccg catccgccgc
     6061 ctgctcgaaa tgcaggggca ggcgtgccgc aacaccgccc aagccatccg gtcgggcaaa
     6121 gactacacat acagcaaacg cctcggacgc gccatcgaag gctgccgcca atcgctgcgc
     6181 ctcctttcag acggcaacga cagtcccgac atccgccacc tgagccgcct tctcgacaac
     6241 ctcggcagcg tcgaccagca gttccgccaa ctccgacaca gcgactcccc cgccgaaaac
     6301 gaccgcatgg gcgacacccg catcgccgcc ctcgaaaccg gcagcttcaa aaacacctgg
     6361 caggcaatcc gtccgcagct gaacctcgaa tcaggcgtat tccgccatgc cgtccgcctg
     6421 tccctcgtcg ttgccgccgc ctgcaccatc gtcgaagccc tcaacctcaa cctcggctac
     6481 tggatactgc tgaccgccct tttcgtctgc caacccaact acaccgccac caaaagccgc
     6541 gtgtaccaac gcatcgccgg caccgtactc ggcgtaatcg tcggctcgct cgtcccctac
     6601 ttcaccccct ccgtcgaaac caaactctgg atcgtcatcg ccggtaccac cctctttttc
     6661 atgacccgca cctacaaata cagtttttcc accttcttca tcaccattca ggcactgacc
     6721 agcctctccc tcgcaggttt ggacgtatac gccgccatgc ccgtgcgcat catcgacacc
     6781 attatcggcg catcccttgc ctgggcggcg gtcagctacc tgtggccaga ctggaaatac
     6841 ctcacgctcg aacgcaccgc cgcccttgcc gtatgcagca gcggcacata cctccaaaaa
     6901 attgccgaac gcctcaaaac cggcgaaacc ggcgacgaca tagaataccg catcacccgc
     6961 cgccgcgccc acgaacacac cgccgccctc agcagcaccc tttccgacat gagcagcgaa
     7021 cccgcaaaat tcgccgacag cctgcaaccc ggctttaccc tgctcaaaac cggctacgcc
     7081 ctgaccggct acatctccgc cctcggcgca taccgcagcg aaatgcacga agaatgcagc
     7141 cccgacttta ccgcacagtt ccaccttgcc gccgaacaca ccgcccacat cttccaacac
     7201 ctgcccgaca tgggacccga cgactttcag acggcattgg atacactgcg cggcgaactc
     7261 ggcaccctcc gcacccgcag cagcggaaca caaagccaca tcctcctcca acagctccaa
     7321 ctcatcgccc ggcagctcga accctactac cgcgcctacc gacaaattcc gcacaggcag
     7381 ccccaaaacg cagcctgaaa aaatttcggc attttgtaaa ggggggcgga ttgtcaggca
     7441 ggttgcaaga tagtggataa ggctttgcgc agggtaaatg cgtagcaact gaaccgtcat
     7501 tccctcaaaa acagaaaacc aaaaacagaa acctaaaatc ccgtcattcc cacgaaagtg
     7561 ggaatccggt tttttgagtt tcagtcattc ccgataaatt gccttagtat tgaatgtcta
     7621 gattcccgcc tgcgcgggaa tgacggtaga gcggtttctg ttttttccga taaattccta
     7681 aaactcaaaa tttcatcatt cctacaaaaa cagaaaatca aaaacagaaa cctaaaatcc
     7741 cgtcattccc acgaaagtgg gaatccagaa tctctaaagc ttcagctaac ctttgaatat
     7801 tgctgttgtc ccacgttcta gattcccgcc tgcgcgggaa tgacgggcta aataatatca
     7861 aaccataaat cccgccaaga aacattattt tcttcaataa gttgcaattt ccaagccctg
     7921 ttccatttct tcaactgttt ttcccgagta attgcgctct ccatcgtagg atgcagttca
     7981 taccaaaccg gcatagtaac gttgtaccgt gatgtaaatc cttcaatcaa atgctcccta
     8041 tgttggtcaa tacgttgcac caaatcagat gtaacgccaa tgtataacgt gccattacgt
     8101 tggcttgcta aaatataaac cgcaggctgc atataatacc cttttgaatt atttaaattt
     8161 atattcccgc gaacaccatc cggtgattac tttaaccctt cgttatcccc atagctttcc
     8221 atcattcccg caactcttcg tcattcccgc gaaagcggga atccagaatc tctaaagctt
     8281 cagctaacct ttgaatattg ctgttgttct aaggtccgga ttcccgcctg cgcgggaatg
     8341 acggcggagc ggtttctgtt ttttccgata aattcctaaa actcaaaatt tcatcattcc
     8401 tacaaaaaca gaaaaccaaa atcagaaacc taaaaccccg tcattcccac gaaagtggga
     8461 atccggttcg ttcggtttcg cttgttttaa gtttcgggta acttccactt cgtcattccc
     8521 gcgcaggcgg gaatccggtg cgttgagttt cagctattta gaataaattt tgaaactcta
     8581 atcccgtcat tcccacgaaa gtgggaatcc ggttttttga gtttcagtca ttcccgataa
     8641 attgccttag tattgaatgt ctagattccc gcctgcgcgg gaatgacgcg tttcatttgc
     8701 cgccccctcc cgaaaaacgc aaaaaaatgc cgtccgaaga cctttcggac ggcatttgcg
     8761 gaaaaaccgg ccgcgcaggc gggtcagaag aagacttcgc gccagcttaa gcgtttgata
     8821 ccgcacatcg ggccggtaat atccaagctg tccaaatcgt tcatcagcag ggtgcgcacc
     8881 ccttttttgg agaagcagcg gttgtttttg ccgggacgcc tgccggcggg gtctataccg
     8941 ctgccgcccg catcgccgtc cgccgtcgtt gaaaatccgt ctgtttccgt ttcatccaga
     9001 taacgcacat taaccggctt gtcgtaaaca tatccgttcg ggcagacggt tttgctgttt
     9061 ttccacatgc agcctatggg gacggacttg ccgccggctg tcttatggcc ggaatattgt
     9121 gcgacatcct tattggcttc cggcacaatc gggcgcgcgc ttctcggagt caatgcgccg
     9181 ccgtcggcgg tattgatgcc caaaatggcg gtttgcgcgc cacatttgtc cgtatccgta
     9241 tatttccgga tggtcacgaa ggcggtacgc aataccacgg tcggtttgac ggtaacgcgt
     9301 tctccttccc tcaatctcac tacccaccct ttgccgttcg atccgtcgga tctcttgttc
     9361 aggaataatg ttttattttc ctcgctaagc acttgctcga gcaacccgcc tcccgtgccg
     9421 ttttgtaccg ttaccttaac cgtcccctta tcgtcgtcaa agataccgta aatatattgt
     9481 ggattcgtac cgactacatc ctcttcactc aaatcgctgc ccgtgccgaa gatgaccacg
     9541 cgtttgtctg ccagtcggga aacggcgggc gcggaggtaa tcggcttccc gccttcgaaa
     9601 atagtgctta cagaccattt actaggatcg gaattgctca aatcaaagcg gtacatattc
     9661 ccgccccggt cgccggcata ggcgatatcg acaatgccgt ccaaatcttt atccaccagc
     9721 gtgggggacg aaagcccgcc tttgccgccg ggcacatcga tttttgtaat cggcgtacct
     9781 aaggtgtctt tcaaatcata tacatacagc gcggttttat tttcttggct gtcaatatct
     9841 ttagccgcat aaccggaggc gaggaaggcg gcgtatttgc cgttgcggat tttgccgatt
     9901 tgcggcgtac cgacggtgta gcctaattcc acttccacgc gatttttgcc gttattatcg
     9961 ccatttttga catcaaacag gggagcggcg gccggataat tttcgttgat tttgcttaaa
    10021 tccaaggcat acgcgcctct gccgcccaaa cccatcgcgc cgaacataaa cacgtgtttt
    10081 tgcccgctca gttcgacttg gcgcaagaca aagccgccgt ccacgccgta gcggtcgccc
    10141 acatagcctt tttcggcaaa ggtgcgcagc tcttgggcga gggtggagtc tttgagagcg
    10201 gaagtgtcgt tatcaaaata ttggcgcggc atcgtgccgg ggatgtagct gagcttcagt
    10261 tcgtagcctc gttcatccgt gccggttttt ttgaagatat gcaccatccc gtcgttcgcg
    10321 gcggttgcca aatacccgcc gaccgccgtt atcgggctgt tgacgatgtc gcccaaatcg
    10381 cgattgccgt tttcgcggat gcggtatctt tggctgtatt tgtctggttt ttggttaatt
    10441 aatttaaatt tattgtcatt atcgggaccc cgaaccgtcc aaggcagcag cacttttttc
    10501 cactcgtcgg cttcaagatt gacgttcgct tccttaacaa tgccgaaagt gccgttgttg
    10561 ccggtgtttc caaaaatgac gacctcatcc ttgcttctat tcagtttgat cagctgtacg
    10621 ccgctatcca atcggatgac ggtctgcctt ccgttgaaac gcggctcttt acttttaatc
    10681 tcattctcac tcaagggttt gagggaatag cggcccggtt tgtttttgtc atcattgcgg
    10741 gaaaggtttt gcaggaaaat tttgctgctc gaactgtcgg ggtaggtaga aaccgaagcg
    10801 gaatacatct gcaccttgcc atcttgcccg ggtccgaacc acagcgcggg ggcggtcagt
    10861 gccggcgaag gggctttggt attattattg gggttcggtt tgttgatgca gcggcctgct
    10921 ttgacttgca ggccgagctt gaccggaatc tgcccggtat ctttaatgtc ccacgtagcc
    10981 caaaaactta gccagcctcg tcctgtcgtc tcgacgcgcg gtctcaaggt gcccaaatcc
    11041 actgttttgc cttgggcgat atcttcaatc ctttttttgt ccagcaggtg cagtttggcg
    11101 ttcagataat aggccacggc ggtatgttcg cgctgccagt tgccaaggga aatagggtgg
    11161 tctcgtgtcg tagagacgat ggtttgatta ttattgtctt cattaaaaga agatatcaat
    11221 ttgttgaatg atttgctatt tttgacggta ttctcgctgc ccaaattgaa agacacgccc
    11281 aaccatggat aatccttcaa tttgtaaatg ggcgaattgt cgcggctttt gtcttcgtat
    11341 atatccagct tgttgccctt tctttgcaac ccttgttgac cgaaggcaaa tttggtctca
    11401 tagacaagtt gggggcattg gttgccgctt ttgcatatga cgtcggcgta gctgtagcct
    11461 gccagcgcga tgcgggtggt acgaatccaa tcgacggcat tgttgcgctc tttcagagcg
    11521 tcggcatcaa aaccggaaac cttgccgtag ggcggcaggt aggtggctgt gccaaaaacg
    11581 gcagtaccgc tttgttggga aacaagggta tcggtattgt tgaatgagac agagcctcct
    11641 cctcctcgtg tgttatgata agtatattcg cgctccctgt ctttgccctt tattgttgaa
    11701 tattgcccat tccgccgtac ctcgggctgg ttttgcgcgt tcatcacaat agcgtattta
    11761 tacgtt
//
LOCUS       DAXFWM010000072        11587 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000072 DAXFWM010000000
VERSION     DAXFWM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.072"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            181..897
                     /locus_tag="ACFI8B_001843"
     CDS             181..897
                     /locus_tag="ACFI8B_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC3834994.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            1046..1948
                     /locus_tag="ACFI8B_001844"
     CDS             1046..1948
                     /locus_tag="ACFI8B_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC3834995.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1991..2765
                     /locus_tag="ACFI8B_001845"
                     /pseudo
     CDS             1991..2765
                     /locus_tag="ACFI8B_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            2877..4565
                     /locus_tag="ACFI8B_001846"
     CDS             2877..4565
                     /locus_tag="ACFI8B_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC3834996.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKDGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            4657..5613
                     /gene="gshB"
                     /locus_tag="ACFI8B_001847"
     CDS             4657..5613
                     /gene="gshB"
                     /locus_tag="ACFI8B_001847"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC3834997.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            5772..6155
                     /locus_tag="ACFI8B_001848"
     CDS             5772..6155
                     /locus_tag="ACFI8B_001848"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC3834998.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            6287..6760
                     /locus_tag="ACFI8B_001849"
     CDS             6287..6760
                     /locus_tag="ACFI8B_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC3834999.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            6885..7988
                     /gene="mnmA"
                     /locus_tag="ACFI8B_001850"
     CDS             6885..7988
                     /gene="mnmA"
                     /locus_tag="ACFI8B_001850"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC3835000.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            8059..9729
                     /locus_tag="ACFI8B_001851"
     CDS             8059..9729
                     /locus_tag="ACFI8B_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC3835001.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9841..11475
                     /locus_tag="ACFI8B_001852"
     CDS             9841..11475
                     /locus_tag="ACFI8B_001852"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC3835002.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2831 a         3154 c         3100 g         2502 t
ORIGIN      
        1 acaaaaacca gtacggcatt acctcgcctt gccgtactat ttttgtactg tctgcggctt
       61 cgtcgccttg tactgattta aatttaatcc actatacttt aatttctcct acacgatttg
      121 aaacacagcc gtcttaaaac atcttaaaaa atttgaaagc gtaaaaatac ggaaaacatc
      181 atgaactttg aaaacgacga cattatccat gcgccgacca cgtcttccct gattctcgaa
      241 gagcggcacg attcggagct gttccgtgtt tacgccctga ttttggacgg cattaccgat
      301 caggtgctgc tgcccggcaa aaagctgacc gaatccgaac tttgccgtca gatggtgtgt
      361 tcgcgcaaca ccgtccgcgg cgcgctgtcg cttttggcgc acgacaagat tgtcgatttg
      421 caacccaaca ggggcgcgtt cgtccacgtt cccgatttga aagaaatgca ggatgtgttc
      481 aatgcgcgca tcgaaatgga gacgatgatt ttgaacatcc tcgccggcct gccggatttg
      541 gaaacgcgcc tcaagccgct ttatgcgatg ataaggtgcg aagaagaggc ctccggcagg
      601 ggcgaccgcg tcggctggaa ccgcctgtcc aatgccttcc acgtcgaact ggcgcgcctg
      661 gtgggcaacg atgtgctgtt cgacattatg aacacgctgt gcgcgcggtc ttccctgatt
      721 gtcgccgtgg cgggcgtgca tcgcgagaaa aaacacgcca tcaatacgca tacgcattcc
      781 gaacaccgcg aaatcctcga cctgctgctg gcgggcaggc gcaaccgcgt ggtcaaaatc
      841 ctgcgccgcc atttgggcaa ctgtatggag cgtttggaaa agactttgga agattgaatg
      901 ccggagcgga taagccgcct gaaccttcag gcggtttttt aatggcgaac ctgatgccgt
      961 ctgaaatatg gatgcgggta tctgcaattt tcagacggca atttttaagc cgcacatatc
     1021 atgcggcaat aaaggagggt aggggatgag cagcctgatg acgttgtttt cggtattggt
     1081 accgatgttt gccggatttt ttatccgtgt tcccaagcct tacctgcccg cttcggacaa
     1141 ggtgctgtcg gttttggtgt atgccgtgct gctgctgatc ggcgtatcgt tgtcgcgcgt
     1201 ggaggatttg ggttcgcggt tgggcgatat ggcgttgacg gttctgtggc tgtttgtttg
     1261 tacggtaggg gcgaacctgc ttgccttggc agtgttggga aagttgtccc cgtggcggat
     1321 agggggaaaa gggaagggcg tttcggtcgg cgtgtcgggc agtgtgaggc agctcggatg
     1381 cgtactgatc ggttttgtgt ccggcaaatt gatgtgcgat atttggatgc cgtctgaaaa
     1441 cgcgggtatg tactgcctga tgctgctggt gttcctcatc ggcgtacagc tcaaaagtag
     1501 cggcgtatcg ttgcggcagg ttttgcttaa ccggcggggc atccggctgt cggtttggtt
     1561 tatattgtca tctctttcag gcgggctgct gtttgccgca tcggcagatg gtgtgtcgtg
     1621 gacgaaaggt ttggcgatgg cttccggctt cggttggtat tccctctcgg gtttggtaat
     1681 gaccgaggct tacggggcgg tatggggcag catcatgctg ctgaacgatt tggcacgaga
     1741 gctgtttgca ctggcattta ttccgctgct gatgaagcgt tttccggatg cggcggtggg
     1801 ggtcggcggc gcgaccagta tggatttcac attgcccgta attcagggtg cgggcggttt
     1861 ggaagtcgtg ccggtagcgg tcagcttcgg cgtggtggtc aatatcgccg ccccgtttct
     1921 gatggtggtg ttttccacgc tgggctgaac gcggtaaaat cggcatcccg atgcaaggaa
     1981 gcagaaaacg atgaaaccga aaatccaaag gcatggagag attttaagcc ttgtccgccg
     2041 gcatcagttt atgtcggtgg acgagcttgc cgccgcattg gacgttaccc cgcagacgat
     2101 acgttgcgac atccgcgagt tggaggaggg cggcagcctg aaacgccatc acggcggcgc
     2161 atcttcgggc ggaaacttgc cggagggtct gcccgccgac cgccaaaccc ggtgtcaaaa
     2221 cgaaaaaaac gccattgccc ggctgattgc ggaacacatc cccgacggtt cgtcgctgtt
     2281 cgtcagtatc ggtacgacca tggaagccgc ggcatcagag ctggtgaagc ggcgcagcag
     2341 cctgcggatt attaccaaca atatccacgt cgcctccgtc gtttcggcac gtacggatta
     2401 cacggtcatc atcacatccg gcgtcgtccg ccctttggac ggcggtatta ccggcgtggc
     2461 gaccgtcgac tttatcaacc agttcaaagt cgattatgcc gtgatgagca cgcacggcgt
     2521 ggaaagcgac ggttcgcttt tggattacga ttacaaggaa gtcagcgtca tgcaggcgat
     2581 gattgccaac gcgcgcgtcc gttttctcgg cgtggatcac agcaaattcc gcagcaacgc
     2641 gctggtcagg ctcggcggca ttaccggcgt ttgacaaagt atttaccgac aggttgcctg
     2701 ataccgcgat gcagaagatg ctgaaagagg cgggggtgga atgcctgatt gccgatgccg
     2761 tctgaacgct atgtcaaagc gcgcaagtcg ggtacaataa acacatcatc aaaccgcttc
     2821 agacggcata cggaacctcc ccaatgccgt ctgaagccat ctgtttaaag aaaaccatgc
     2881 tcaataaaga ccaattcgcg gacaaccatt tcatccgcac catcatcgaa gacgacctca
     2941 aaagcggcaa acacgaagcc gtccaaaccc gttttccgcc cgaacccaac ggctatttgc
     3001 atatcggaca cgccaaatcc atctgcctga acttcggttt ggcgtatatt tacgacggtt
     3061 tgtgcaacct gcgtttcgac gacaccaacc cggaaaaaga aaacgacgaa tacgtcaacg
     3121 ccatcaaaga agacgtcgag tggctcggtt tccattgggc gggcgagccg cgtttcgctt
     3181 ccgactattt cgaccggctt tatgactacg ccgtcggttt gattaaagac ggaaaagcgt
     3241 atgtcgatga tttgacaccc gaagaaatgc gagaataccg cggtacgctg accgaagcgg
     3301 gtaaaaacag cccttaccgc gaccgcagta tcgaagaaaa cctcgacctg ttcacgcgta
     3361 tgaaagacgg cgagttcccc gacggcagca aaaccctgcg cctgaaaatc gacatggcgg
     3421 caggcaacat caatatgcgc gatcccgtca tctaccgcat ccgccgcgcc catcaccaca
     3481 acaccggcga caaatggtgc atctacccga tgtacgatta cacgcattgc atttccgatg
     3541 ccatcgaagg catcacgcat tccttgtgca cgctcgaatt tgaagcgcac cgtccgcttt
     3601 acgactgggt gttggacaac atccccgcgc cgcacgccac ccgtccgcgc caatacgagt
     3661 tttcccgttt ggagcttttg tacaccatta cctccaaacg gaaattgaat cagttggttg
     3721 tggaaaaaca cgtttccggc tgggatgatc cgcgtatgcc gaccatttcc ggtatgcgcc
     3781 gccgcggcta cacgcccgaa ggggtgcgcc tgtttgccaa acgcgccggt atttccaaat
     3841 ctgaaaacat cgtcgacatg agcgtgttgg agggtgcgat tcgcgaagag ctggaaaact
     3901 ccgccccgcg cctgatggcg gttttgaacc cgctcaaagt gaccctgacc aactttcaag
     3961 ccggcaaaac ccaaagccgc cgtgccgcgt tccatccgaa ccacgaagaa atgggcgatc
     4021 gcgaagtacc tgtttcacaa accatctaca tcgaagccga cgactttgcc gaaaatccgc
     4081 ccaaaggctt caagcgtctg attcccggcg gcgaagtacg cttgcgccac ggctatgtca
     4141 tcaagtgcgg tgaagtcgtc aaagacgaag caggcaatgt ggttgaactc aaatgcagca
     4201 tcgaccacga caccttgggc aaaaatccag aaggccgcaa agttaaaggc gtgattcact
     4261 gggtttccgc cgaacacgcc gccgaaatca aagtccgtct gtacgaccgc ctctttaccg
     4321 tcgagcgtcc cgatgccgtg cgcggcgaag acggcgaata cctgccgttt accgatttcc
     4381 tcaatccgga atccgttaag gaaatcactg cttacgccga acctgccgcg aaagatttgc
     4441 cggcggaaag ccgttggcag ttcgagcgca tcggctattt tgtgaccgac cgccaagacc
     4501 acggcaaaga cacgccggtg tttaaccgca cggtgacgtt gaaagattct tggcagccta
     4561 agtaaacccc atccttgccg tctgaatatt gttcgggcgg catttctcct ttacccgcga
     4621 aatgcggcac attcggcaca ccggaaagga aatatgatga aagtcctctt tatcgccgac
     4681 ccgatggcaa gtttcaaaac ctacaaagac accacctacg cgatgatgcg ggaaatggca
     4741 aaacgcggct ggcggctgtt tcataccttg agcggggaat tgtctgtaaa cggcggtttg
     4801 gtaacggcac aggcatcggc atttgaattt tcgggtgcaa aaaacgatga tgaccatgaa
     4861 tggtttaaag cggcggacaa agttcagacg gcattaaaag catttgatgc cgtgattatg
     4921 cgtaccgatc cgccgttcga tatgcaatac ctttactcca cccaattact gacgctggcg
     4981 gaacagcagg gcgcgaaagt gttcaacagc ggacgggcga tgcgcgactt taacgaaaaa
     5041 ctggcgattt tgaatttcag ccgctttacc gcgcccacgc tggtaacgac ccgttccgcc
     5101 gatgtccgcg catttttgaa agaacacggc gacatcatcg tcaaaccgct cgacggcatg
     5161 ggcggcatgg gcatcttccg cctgaccgaa aaagacccca acatcggcag catcctcgaa
     5221 accctgatgc ggtttgattc ccgcaccatt atggcgcaac gctacattcc cgaaatcgta
     5281 cacggtgaca aacgcatctt gattatcggc ggcgaagtcg tcccctatgc tttggcgcgt
     5341 atcccgcaaa acggcgaaac acgcggcaat ctggcggcag gcgggcgcgg tgtggcgcag
     5401 gaattggacg gacgcgaccg ggaaattgca gagactctgg ctcccgagct taaacggagc
     5461 ggcatcctgc tggccggttt ggacgttatc ggcagcaacc tgaccgaagt caacgtaacc
     5521 agcccgaccg gattccaaga aattatgaaa caaaaaagtt tcgacgtggc ggcaatgttt
     5581 gccgatgccg ttgccgcgtg gtcggtacgt taaaccgatg ccgtctgaaa ggcttttgct
     5641 tcgtaaccgc ttggtttgtt cgggcaggca gggttttccc tcagccggca gggatgcgct
     5701 taatgccgta cggacacccc tccgccccgt tttcagacgg catatattga ggacattttg
     5761 aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa agcggcatca
     5821 ggcgcgtcat caacgcattc ggctattcga tagacggcat cgccgccgcc taccgttacg
     5881 aagcggcatt ccgtcaggtt ttgtggctga acgcgctgct ggtgtgcgcg gcattttttt
     5941 gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg tcggtcattg
     6001 tcgaactgtt caacaccgcc gtcgaagccg ccgtcgatca tacttcgacc gaaaaacacg
     6061 agctggccaa acgcgccaaa gatgcaggtt ctgccgcaca actggttgcc atgctgatgt
     6121 tggcggcggt ttggctgtcc gccctgttcg ggtaaaacgc ttgcagcagg atttgtaatc
     6181 ctttaggatt agtatttatt attcacttaa tctacatcaa atttccgagc agtatttgta
     6241 tgtaagatta agcacattcc ccgtctgata ttaaaggagc aggaagatga aaaaattatt
     6301 ggcagccgtg atgatggcag gtttggcagg cgcggtttcc gccgccggag tccatgtcga
     6361 ggacggctgg gcgcgcacca ctgtcgaagg tatgaaaatg ggcggcgcgt tcatgaaaat
     6421 ccacaacgac gaagccaaac aagacttttt gctcggcgga agcagccccg ttgccgaccg
     6481 cgtcgaagtg catacccaca tcaacgacaa cggcgtgatg cgtatgcgcg aagtcaaagg
     6541 cggcgtgcct ttggaggcga aatccgttac cgaactcaaa cccggcagct atcacgtgat
     6601 gtttatgggt ttgaaaaaac aactgaaaga gggcgacaag attcccgtta ccctgaaatt
     6661 taaaaacgcc aaagcgcaaa ccgtccaact ggaagtcaaa accgcgccga tgccggcaat
     6721 gaaccacggt catcaccacg gcgaagcgca tcagcactaa tctgctgaaa atatttgaaa
     6781 tgccgtctga aaaagcccgg gctttcagac ggcattttta tgcccgcctt taaaatgtgt
     6841 taaaatccgc ctttaaaaac cgccgtttcc aagccatcct gcgtatgaat acgacatcaa
     6901 acacttccaa tatcatcgtc ggtctttccg gcggtgtcga ttcttccgta accgccgccc
     6961 tgctcaagca gcagggttat caagtgcgcg gtgtgttcat gcagaactgg gaaaacgacg
     7021 acaacgacga atattgcagc atcaaacagg attcgttcga tgccatcgcc gttgccgata
     7081 ttgtcggcat cgacatcgac atcgttaatt tcgccgcgca atataaagac aaagtttttg
     7141 cttattttct tcaggaatac agtgcggggc gcacgccgaa tccggatgtg ttgtgcaatg
     7201 ccgaaatcaa attcaaatgc tttttggact acgccgtagg gcagggcgcg gataccattg
     7261 ccaccggaca ctatgcgcgc aaagaagtcc gcaacggcgt gcattacctg ctcaaaggtt
     7321 tggatcgaaa caaagaccaa agctattttc tctaccgcct caagcctttc caactcgaac
     7381 gcgcgatttt tccgttgggc ggtttggaaa aacccgaagt gcgccgcctt gccgccgaat
     7441 ttaatttgcc gactgccgct aaaaaagaca gtaccggcat ctgtttcatc ggcgagcgtc
     7501 cgttccgcga gtttctgcag aaatacttgc cgaccgacaa cggcaaaatg gtgacgcccg
     7561 aagggaaaac catcggcgaa cacgtcgggc tgatgtttta cacattgggt cagcgcaaag
     7621 gattgggcat cggcggcgcg ggcgaaccgt ggtttgttgc ggctaaagat ttgacgaaaa
     7681 acgaactcat cgtcgtacaa ggacacgacc atccgctgct ctatacccgc agccttgtga
     7741 tgaacgattt gagtttcacg ctgcccgaac gtccgaaggc aggacgctat acctgcaaaa
     7801 cgcgttaccg tatggcggac gcgccttgcg aattgtgcta tttggatgat gaaaccgccg
     7861 agctggtgtt tgacgaaccg caatgggcgg ttacgccggg tcagtccgcc gtgctgtacg
     7921 acgtcgacat ctgtttgggc ggcggcatca tccaaacgac cgacaaaccc gtcatcatca
     7981 cgcgataaag gtaatgccgt ctgaaacggt tttcagacgg cattgttccg ctcaattcca
     8041 ctttaaagac cgatacctat ggaaaaaatc tggttagaaa gctacgagaa gggcgtcagt
     8101 gccgaaatcg acatcacgca atacaattcc gtcagcgacg tattccgcca aagcgtggaa
     8161 aaatttgccc gtctgcccgc ttttcaaaat atgggcaaaa cgctcactta tgccgaaacc
     8221 ggcaaactgg cgaccgattt cgcctcttat ctgcaaaacg tcctcaagct gccgcgcggc
     8281 gaacgtgttg ccattatgat gccgaacgta ttgcaatatc cgattgccct tttcggtatt
     8341 ttgcaggcag gtttggtggc ggtgaacacc aatccgctct atacgccgcg cgagttggag
     8401 catcagctga aagacagcgg tgcgaccgcc atcatcgttt tggaaaattt cgccaacacg
     8461 ctggagctgg tgctgccgcg cacgcagatc aaacacgtca tcgtcgcctc cgtcggcgaa
     8521 atgttcgggc tgcttaaagg ttcgctgatc aatttcatca tccgaaaaat caagaaaatg
     8581 gttcccgaat accgtattcg ggaaaccgtt tcctttcaga cggcattgaa agagggggcg
     8641 aagcacgttt tccaacctgt cgcattaaac cgcgaagata ccgcattgtt gcaatacacg
     8701 ggcggcacga caggcgttgc cagaggcgcg gtgctgagcc acggcaacat ctgcgccaat
     8761 atgcttcagg caaaagaatg gattaaaaac caattgcgcg agggaaaaga aaccgttatc
     8821 gccgccctgc cgctgtacca catcttcgcc ctgacagtga atctgatgat ttttgccaat
     8881 gccggctcga aaattgtcct gattgccaac ccgcgcgata tgaaaggctt tatcggcgaa
     8941 ctgaaaaagc agcgggttaa cgtatttatc ggcgtgaaca cgctgtttaa cgcgatggtc
     9001 aaccggcccg atttcgccga agtcgatttt tcaggattgc ggctgacttt gggcggcggt
     9061 atggcgaccc aaaaagccgt tgccgaaaaa tggaaaaaaa tcaccggcac gcccatcgtc
     9121 gaagcctacg gtttgaccga agccagcccc ggcgtgtgct gcaacccctt aaacatcgaa
     9181 tcatacagcg gcagcatcgg tttgcccgtc ccgtccaccg aagtcgaact gcgcgacgca
     9241 aacggcaaag aagtccccgt tgggcagccg ggcgaattgt gggtaaaagg cccgcaagtg
     9301 atgcaaggct actggaaccg ccccgaagaa accgccaaag ccatagacgc gtgcggcttt
     9361 ttggaaaccg gcgatattgc cgtgatggac gaaaaaggcc gtctgaagct ggtcgatcgc
     9421 aaaaaagacc tcgtcgtcgt ttccggattc aatgtttatc cgaacgaaat cgaggaattc
     9481 atcgcgcacc acgaaaaagt tatggaagtt gcgtgtatcg gcgtacccga cgaaaaaacc
     9541 ggcgaggcac tcaaagtgtt cgtcgtcaaa aaagacccgt ctttgaccaa agaagaactc
     9601 accgctttct gccgtaccgg tctgaccgca tacaaagtgc cgaaagacat cgaattccgc
     9661 gacgagttgc ccaagtccaa tgtcggcaaa atcctgcgcc gcgagttgcg ccaaagtacc
     9721 gggaaataaa gaaaagatac cgtctgaaaa cagccgtcca ccgttcagac ggcatccgtc
     9781 cgtttgcaag aaccgcgcgc ttcacgttaa aatcacgcat tccaatacgg gtattccatc
     9841 atgaccaaat tcattttcgt aaccggcggc gttgtctcct cactgggtaa aggtatcgcc
     9901 gccgcttcta ttgccgccat cctcgaatcg cgcggcttga acgttaccat gctcaagctt
     9961 gatccttata tcaacgtcga tcccggcacg atgagcccgt tccaacacgg cgaagtgttc
    10021 gtaaccgacg acggcgcgga aaccgacctc gacttgggac actacgaacg tttcatcgat
    10081 tccacgatga cccgccgcaa cagcttcagc acgggtcagg tgtacgaaaa cgtcatcgcc
    10141 aaagaacgac ggggcgacta tttgggcggc acggttcaag tcatcccgca cattaccgac
    10201 gaaatcaaac gccgcatcca cgaaggcgcg gcgggttacg atgtggcgat tgtcgaaatc
    10261 ggcggcacgg tcggcgacat cgaatcgctg ccgtttttgg aagccatccg ccagatgcga
    10321 agccagttgg gacgcaacaa caccctgttc gcccacttga gctacgttcc ctacatcgcc
    10381 gccgcaggcg aaatcaaaac caagccgacc cagcacaccg tgaaagaaat gttgagcatc
    10441 ggcttgcaac ccgacatcct gatttgccgt atggacagga aaatgccggc agacgaacgc
    10501 cgcaaaatcg ccttgttctg caacgtggaa gagcgcgcga ttgtcggcag ctacgatgtg
    10561 gacagcatct acgaatgccc cgaaatgctg cacgaccaag gcatcgacaa catcattacc
    10621 gagcagttgc agcttaacgt gcagcaggcg gatttgaccg cgtggaaaaa aatcgtccac
    10681 gccgtcaaaa acccgaaaca caccgtcaaa atcgcgatgg tcggcaaata cgttgatttg
    10741 accgaatcct acaaatcatt gattgaagcc ttgaaacacg cgggcatcca taccgaaacc
    10801 gatgtgcaga ttaccttcgt tgacagcgaa agcatcgaga aaaacaaggg cgacgtttcc
    10861 gtactcaaag atatggatgc catcctcgtt cccggcggct tcggttcgcg cggcgtggaa
    10921 ggcaaaatcg ccgccgtgcg ctacgcccgt gaaaacaacg tgccatactt gggcatctgc
    10981 ctcggtatgc agattgcgct gattgaatac gcccgcgacg tggcaggttt gaaaggtgcg
    11041 aattccactg agtttgactt gaaatgcgct gcccccgtcg tcgccctgat tgacgaatgg
    11101 caaaccgccg acggcagcgt cgaaacccgt gacgaatccg ccgatttggg cggcacgatg
    11161 cgtttgggcg cgcaagaagt cgaattgaaa gcaggcagcc tcgccgtcaa aatctacggc
    11221 agcggacaca ttcgcgaacg ccaccgccac cgctacgaag tcaacaacaa ctacgtttcc
    11281 gcgctggaac aggcaggttt ggtcatcggc ggcgtatccg ccggacgcga acgcttggtc
    11341 gaaaccatcg aactgccgaa ccatccttgg ttcttcgcct gccagttcca tcccgagttc
    11401 acgtccaacc cgcgcaaagg gcatcctttg ttcaccgcgt ttgtcaaagc cgcgttgaac
    11461 aataaaaaag cctgataaag cgttacttga tgatcaaaat gccgtctgaa agcctgacaa
    11521 agactttcag acggcatttt cgcaaatcgg ggatcataaa agccatcaat ttaatttggg
    11581 tctgtac
//
LOCUS       DAXFWM010000073        11474 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000073 DAXFWM010000000
VERSION     DAXFWM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.073"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            245..1549
                     /locus_tag="ACFI8B_001853"
     CDS             245..1549
                     /locus_tag="ACFI8B_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC3835003.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(1643..2938)
                     /locus_tag="ACFI8B_001854"
     CDS             complement(1643..2938)
                     /locus_tag="ACFI8B_001854"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC3835004.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLCQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(3065..3952)
                     /locus_tag="ACFI8B_001855"
     CDS             complement(3065..3952)
                     /locus_tag="ACFI8B_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3835005.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(3952..4917)
                     /locus_tag="ACFI8B_001856"
     CDS             complement(3952..4917)
                     /locus_tag="ACFI8B_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3835006.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(4933..6057)
                     /gene="potA"
                     /locus_tag="ACFI8B_001857"
     CDS             complement(4933..6057)
                     /gene="potA"
                     /locus_tag="ACFI8B_001857"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC3835007.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(6335..6604)
                     /gene="rpsO"
                     /locus_tag="ACFI8B_001858"
     CDS             complement(6335..6604)
                     /gene="rpsO"
                     /locus_tag="ACFI8B_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC3835008.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(6685..6819)
                     /locus_tag="ACFI8B_001859"
     CDS             complement(6685..6819)
                     /locus_tag="ACFI8B_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835009.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(6809..7744)
                     /gene="secF"
                     /locus_tag="ACFI8B_001860"
     CDS             complement(6809..7744)
                     /gene="secF"
                     /locus_tag="ACFI8B_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC3835010.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(7748..9601)
                     /gene="secD"
                     /locus_tag="ACFI8B_001861"
     CDS             complement(7748..9601)
                     /gene="secD"
                     /locus_tag="ACFI8B_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC3835011.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(9812..10078)
                     /gene="yajC"
                     /locus_tag="ACFI8B_001862"
     CDS             complement(9812..10078)
                     /gene="yajC"
                     /locus_tag="ACFI8B_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC3835012.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            10209..11327
                     /locus_tag="ACFI8B_001863"
     CDS             10209..11327
                     /locus_tag="ACFI8B_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC3835013.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPEHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         2833 a         3035 c         2946 g         2660 t
ORIGIN      
        1 taaattgttg acaatccata cgcccgcata tgcgccgcct aaggataaat cctcccgccg
       61 gacaacgggg gcaagggatg ggatgcgata tttccatatt caaacaaggg atttgtttca
      121 cgcacagggc ggcacatcgg caaaatcccc gcgccggtcc ggcagggctt gcgcccatcc
      181 cggacaagcc ccgaccccgc ctttccgaaa gacgggccca accattaagg aaactttaat
      241 caaaatgaaa aaacacatat gggcggcatc tttgctgccg gcatccctat cggcagaacc
      301 tttaaactgg tggaaacctt attccgccgt caattcgggc gataccgcct gggtgatgac
      361 tgcggctgcc ttggtactgt tgatgacgct tcccgggctg gctttattct acggcggtat
      421 ggtgcggaaa aaaaacctgc tctcgacgat gatgcacagc ttttccatcg cgacattggt
      481 gggcatcctt tgggtcgccg tcggctattc tttggcgttc acgccgggaa atgcctttat
      541 cggcggtttg gggcgcgtat ttttaagcgg gatgcagata gacgctgccg cacggatgct
      601 gaccgtgtcg cccaatgcgc cgactgttcc cgaaccggta tttatgtttt ttcagatgac
      661 gtttgccatt atttcgactg ccattattac cggcgcgttt gccgaacgaa tgaaatattc
      721 ggcaatgatg ctgttttcgg gcatatggtt tttattggtt tatgtgccgg gcgcgcattg
      781 ggtgtggggc ggcggcttta tgagcaaggg cggcgtattg gattatgccg gcggtacggt
      841 ggtgcacatc aatgccggta tcgcgggact cgtcgccgcc ttggttttgg gcaggcgcat
      901 aggctacggg cgcgaggcga tgcctccgca caatatggcg atgacgctga ttggcgcggc
      961 aatgttgtgg ttcggctggt tcggctttaa cgctggttcg gcgcttgcgg cagacgcggc
     1021 ggcggggatg gcgatggcgg taacgcaggt gtcggccgta ttcggcgcgg caggctggct
     1081 tgcctgcgag aaaatagcgg gacacaaacc ttctgctttg gggctggctt ccggcgcagt
     1141 ttccgggctg gtcggcatca cccctgccgc cggctttacc ggtccgtcgg gcgcggccgc
     1201 catcggtata ttgactgccg ccgcgtgctt tgtgtccgtc accgtcgtca aacacaaatt
     1261 gcgttacgat gattctttgg acgctttcgg catacacgga ttcggcgggc tggtaggcgg
     1321 aatattgacc ggcatctttt tcgacaaccg cattttcggt ggggatgcgg cagtttggca
     1381 gcagttgtgg atacaggtaa aagacgggtt cattatggcg gcatacagcg ggctgatgag
     1441 ttgggcgatt ttgaaggctg tggggaaaat ctgcggcggt ctgcgcgtcg gcaaggatgt
     1501 cgaacgcgaa ggtttggatc tgaatatcca cggcgaacac gtggaataag gacggctatg
     1561 ccgtctgaag cctgaaaatc gggtttcaga cggcattttt cacgtttgcc gccgatgggg
     1621 ataaacatac tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga
     1681 ggaaagaacc gagtttgcgc aaaaacggtt gcaggataat attcggctgg cacaactgtt
     1741 caaacggttt cagacggcat tcgtccccta aaattgcttc ggcaaccgcc aggcctgcaa
     1801 tgcccgttat cgccatcccg tgcccggaat aaccttgcgt ataaaaaaca ttcgaggtca
     1861 aacgtccgaa atgcgggaca aggttggcgg taatatcgca ctccccgccc cacgaatatt
     1921 cgattctaac atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg
     1981 tcatacgcgc aggattgtcg ataaattcgt tatccttacc gccgaaaagc agcctgccgt
     2041 ccgcgctgag gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttgc
     2101 ggataagccc ttttgtgcgc gcgcccaagg gttcggtggc aataataaag gtgctgacgg
     2161 caatcgcctt gcgttccaaa ggccggaata tcgggttcaa accgacataa gtattgacgg
     2221 catagaccac atttttacac tcgacgctgc cttcgggcgt gtaaaccagc caaccgtttt
     2281 gatacggttc gatgcgcgtc atcggggatt gctcgaaaat ctgcgcgccg gcttcggcag
     2341 cggcgctggc aacacccaac gtgtaattga gcggatgaag atgcccggac aagggatcga
     2401 actgtgcgcc ttggtacata tcgctgtcaa gctgctgttt caactcggct ttatcccaaa
     2461 gttgataatg actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt
     2521 cccaatgctg cggacggacg gcaaccgtgg cataaccgcg ctgccaatcg caatcgatgg
     2581 catgtttgcg gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc
     2641 attgcgcctg ctccaagccg acctgttttt caatttcccc cataccgcag gcgtagtcgc
     2701 tgataacctg cccgccactc ctgccggacg cgccgaagcc gatacgtgcg gcttccaaaa
     2761 cgacggcttc atgtccgtgt tccgccagcg gcaatgcggt acacaaaccg ctcaaaccgc
     2821 cgccgataat gcaggtttcg gctttcagac ggcattggag tttcggataa acagtatgcg
     2881 gattaaccga actaaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg
     2941 tgtttgcttt atcgggtata ttttcggacg gaatgataca ggctgtcggg ccatatcgtc
     3001 caaacagaaa atcggttgaa aaaaaacagg ctgacccagt catgcggtca gcctgcctta
     3061 ttaattaatt tgctttctcg gcagccaatt tttcttggcg gtaggcttct gccgcttctc
     3121 ggttgcgctt ggttgcctgc ctcatcatcc aataattgac gatgatgacc aatgttccga
     3181 tgatgccgat caggatggtt gccaagacat tcatctgagg atcgagaccc aacttgatct
     3241 tggagaaaat aacctgcggc aatgtggatg aaccggggcc ggagaggaat gaggtaatta
     3301 ccaaatcatc caaagacaag gtaatgccga gtaggaagcc tgaagcaatg gcaggggcaa
     3361 tcaaaggcaa agtgatgaca aaaaagattt tcagcggacg cgcgcccaag tccattgcgg
     3421 cttcttcgag cgactggtca agctcgacca gccgcgaacg gatgacaacg gtaatgtatg
     3481 ccatgcacag cgtcgtatgt ccgaggaaga tggtgaaaaa gccgcgatcg aagtagagat
     3541 gttgcaacca ttcgctgccc tgcaaaaata tctgcacctg aataatcagc agcagcatag
     3601 acagaccggt aatcacgtcg ggcatgacca tgggcgcgga aatcataccg gcaaacagcg
     3661 tgctgccgcg aaagcgtttg atacgcgcca tcgcatagcc cgccagcgtg cccaaaacga
     3721 cggcggcaag cgaagacaca acggcaatcc gcagcgacag ccaagcggct tccaaaatgg
     3781 tgtcgttttc cagcaatgcg ccgtaccact tggtcgaaaa gccgccccaa acggtaacaa
     3841 gtttggattc gttgaacgaa tagatgacca aaacaaccag cgggatatac agaaacgcca
     3901 gcgatagtgc caacatcagt ttcaagaacc aagataattt ggatttctgc attatttggc
     3961 tccttcttcc aattcgcggt tttcatagtg ctggaacagg gcaatcggta caaccagtag
     4021 cgcgaccatc acgacggcga cggcagaagc caacggccag ttgttttgat caaagaatgc
     4081 ctgccacaat actttaccga tcatcaggtt ttccgaaccg ccgaccagct cgggaatgac
     4141 gaactcaccg acagcgggga cgaaaaccag catcgaacct gcaataatgc cggttttcga
     4201 caaaggcagg gtaatcgtca agaacgattt gaccggtccc gcgcccaagt cggaagcggc
     4261 ttcgagcagg cggctgtcca gtttcaccag ctgcgtgtaa agcggcagaa tcataaacgg
     4321 cagataggca taaaccatta ccaaatttaa cgaaaacgca ttatagaaca aatctaatgg
     4381 ttctctgata atacccatct taatcaataa gttgtttatg atgccgttat gcccgagcaa
     4441 acccatccac gcatagacac gcagcaggaa cgatgtccaa aaaggtaaca tgatggcaag
     4501 cagcaaacca ttgcggacgg aaggattggc acgggaaatc gcataggcgg tcgggtaacc
     4561 gaccaacagg caaatcaccg tcgtcgtcag cgcagtctta attgaagacc aataagtcat
     4621 cagatagata ttactgtttt cactgtcgcc gaaggggtta agggtatgcc aaaaattttg
     4681 gaaaatatcg gcatagtttt gatagctgac ggcgatgttc aggcgaccca agtcctcatc
     4741 tatcgtcgtt aaaggagtaa acggtggaat ggcgatttct tgttcggcaa agctgatttt
     4801 cagcacgatg gcgaacggaa tcagaaacag caccaaaagc caaatatacg gtacggcaat
     4861 caccgcacgc tgccccggac ggcggaacag tttgtttttc agtttattaa ggttcatctc
     4921 actcccctta aatcaacgga acaacggagt cggttggttt tccggccagc tgatatagac
     4981 ggtttcatcc caagtcggcg gcgtgatgtt acgcacatac cagtaaggtg caggaacttg
     5041 gcttttgacg acgcgcccgt tggcgagttt gatatggtat atggcgaagc tgcccaaata
     5101 ggcaatttct tttaccgtgc ctttcgccca gttgtagtcg cccaaatatt cgggtttttc
     5161 tttatataaa tcaatatcct ctggtcgaat actaacccaa agatcctgct cgctcgggcc
     5221 acccaaacca tggtcgatac ggacgtggtt ttccaaacct tcgcattcga taacggcata
     5281 gtcggcatga ttttcaatca ccacaccgtc aaagatgttg gtttcgccga taaactcggc
     5341 agtgaagcgg ctgttgggat agtcgtacac gtcgctgggc gtgccgacct gctgcaactg
     5401 accgtcggac ataatggcga tgcgggtcgc catcgtcatc gcctcttctt ggtcgtgcgt
     5461 aaccataatg caggttacgc cgacttgttc cagcgtgttg accaactcga gctgggtttg
     5521 ctggcgcaat ttcttatcca acgcccccaa aggttcgtca agcaacagga ttttcggacg
     5581 ttttgccaaa ctgcgcgcca aggcgatacg ttgctgctga ccaccggaca attggtgcgg
     5641 tttgcgtttg gcaaatttgg tcatctgaac cagacgcagc atttcttcga cgcgcgcatc
     5701 gatttcgcct ttaggcattt tgtcctgttt cagaccgaag gcaatgtttt gctccaccgt
     5761 catatgcgga aaaagcgcgt aactttggaa catcatattg atggggcggt catagggtgc
     5821 aagtttggta atatcttgac catcaaggat gatttttccc tgattgggac tttccatacc
     5881 cgccagcata cgcagcagcg tagattttcc gctgccggaa ctgcccaaaa gggcgaagat
     5941 ttcgttttga taaatgtcca agtcgatgtt atcgacagcg taattgtcac caaacttttt
     6001 caccaaacct tggatttgca gataaggttt ggctgaagac gctgtggttg cggtcataat
     6061 ggcaatactc caataaaaag acgagtaccg gcaaaacgga ttttcgaatg ggtgataaaa
     6121 agctgtttga ttgctggcgg gagtcgcaac gtctgatgcc gtctgaaact cttgtgaagc
     6181 gcacgggcag catgaaataa aaaggggttc taaagaaccc cgcattatat tagtttataa
     6241 ggtttttagg gtaataaata tgggtttgag cttgtcggaa agcaacagaa aagaaaacac
     6301 cgcccatttt tctgggcggt gtcggaaagc gcaattattt acgcagaccc aagcgggtaa
     6361 tcaacgcgcg atacgtatcg ggctgggtac ggcgcaaata ggccaacagg cggcggcgtt
     6421 ggctgaccat tttcaacagg ccgcgacggc tgtggtggtc tttggggttg gctttgaagt
     6481 ggggggtcag atcgttgatg cggaaagtca acagggcaac ttgtacttca gaagagccgg
     6541 tgtcgccttc tttacgttgg aaatctttaa cgatttgtgc tttttgttct acggtcagtg
     6601 ccataatgga aaaactccga aaaataaaaa gaaccccaaa tgggattcgg acaagtttgc
     6661 caagcctcga caacggcaaa ctccctattc ctgaaacagg aacagcgcat tatcacacaa
     6721 tttttgctgt tctgcacgat attttgcgat gtcggcgaag tgccgtctga aaacggagac
     6781 atcttccggt ttcagacggc atttgacatc agaccacaat ttcttccttc tgtttcgctt
     6841 ccttggcgat attgtcgcgg ctcaaaccga acatcagcaa gagcgggctg gcaaccaata
     6901 cggaagagta gatgccgaac acgataccga tggtcagtgc catagaaaag ccgtgcaagg
     6961 ctgcaccgcc gaacaccagc atagaaacaa ccatcgcctc ggtcgaaccg tgggtaatga
     7021 tggtgcggct catcgttgcg gtaatcgcgt tgtcaatgac ttccggcacg gtatgtccgc
     7081 gcatcgccgg cttgcggaaa ttttcacgga tacggtcgaa gacgacgacg gattcgttca
     7141 cagaatagcc caatacggca aggatacctg ccaagacggt cagcgaaaat tcccattgga
     7201 agaaggcaaa gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata
     7261 cggcaaaacg ccattcgaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac
     7321 ctaaagccat caatccatta tttaccaatt cctcaccgac ttgcgggccg ataaattcga
     7381 cttggcgcaa agtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca
     7441 actgtgcgga agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac
     7501 ccaatgcctg aacctgtaca tcacccattt tcagcgtatc gaggcgttcg cgcatcttat
     7561 tgacatccgc accctgctga tattggactt ccattaccgt accgccggta aattcgacag
     7621 agaaattcag gcctctggca accaaaaaga atacggcggc aataaaggta accaacgaaa
     7681 tgaaggttgt cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt
     7741 ccatagctta ctccttacct gccgcagttt cggcttcagg tttccatacc gcaccgatgg
     7801 aaatattctg caatttgcgg cggcggccgt accacagatt gaccaacgca cggaatacga
     7861 cgacggacga atacatcgaa gtcagaatac ccaagcagtg tacgacggca aaacctctta
     7921 ccggtccgga accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttcgaat
     7981 caacaatggt cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc
     8041 cggcacgcaa ttcctctcgg atacgttcgt taatcaagac gttggagtcg attgccatac
     8101 ccaaagtcaa cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca
     8161 aaataccgat tagaaacagt atgttggcac tcaatgcaat agtagaaaag aaacccatca
     8221 ggcggtaata aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga
     8281 agcctttttc gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct
     8341 gcatcggtgc ggcaagagaa ccggcacgca acagcaaaga cgtatcgttg gcttcggctg
     8401 tcgtcatgct tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggtg
     8461 cggttacaac ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat
     8521 ttgcggcagt cagttcgccg aaaatgctgc cgcccgcgct gtccaagctc agactgacgg
     8581 caggtgcgcc catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg ccggtcagct
     8641 cgacctgttt gctgatcagc agagtttcgg gatgctcccc gccgcttgaa agcagctcat
     8701 aaccgctcgg cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca
     8761 tgcggacttc caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcttgca
     8821 cgccgggaag ctgtacgaca atgcggtcca aacctgactg ttggataatc ggctctgcca
     8881 cacccaattc gtttacacgg ttatgcagtg tagtgatgtt ttgcttcacc gcatctgatc
     8941 gtactttatt caccgcctct tccgaaagcg ccaatacaat attgctgcct tctgaattta
     9001 acgttgcctc aggaaacagc ttgagtaact gaggcagtgc cttttgcaca tcgccggcat
     9061 cctgcaaagg gacggtcagg ctgttttcag cctgacgcac cgtgccgctg cggatttttt
     9121 cacggcgcag ttcgcggcgg atgtcgcccg aataacgctc aaacgttttc tgcatcgcgg
     9181 ctttcatatc gacctgcatg gtgaaatgca cgccgccgcg caggtccaaa cccaaaaaca
     9241 tcggattggc tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg
     9301 taatataccc ttcgcccaaa gtgttttcga taacgtcgcg cgctttaagc tgcgtttctg
     9361 tgtctttgaa acgcactttc agtgaattat ccacaacaaa catcccgtcg gtctgaatgc
     9421 ccgcgttttt cagcgcagca tccactttgg attgagtctg ttcgttgatg atgatggctt
     9481 gtcggttggt cgatacctgc acggcgggcg tttcgccgaa taggttgggc agcgaataca
     9541 ctgcggcaac cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca
     9601 tcattgttcc ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc
     9661 agaagaggca aaacccctcc ctgccaggca agaccgaaag gcagcatctt gaatatgccg
     9721 ccctgcgtgt cggaacatgg tcaagccttc ggttggaatt caaaacaaag tgccgcattc
     9781 gggctttccc gatgcggctt gtcggcacaa atcaatcgac ttttgcggca atcgcattgc
     9841 gttccacttc gacctcgatt tttgtaccct gtccgatatc cacggtaaaa aactgttcgc
     9901 cgactctggt taccttaccc ttgaaacctg ccgccaagac cactttgtcg ccggctttca
     9961 aggcggcaag cattgcctga tgcgctttga atttcttttg ttgggggcgc atgatcagga
    10021 agtagaacac caccataatc aacaccaaag gagcaaattg tgcaacagct tgattcataa
    10081 tttatccgtt ctttctaaat atgattgaaa atcgagcggg gtatataata gcataagacc
    10141 gtaaacaata tatcgggttt gccgcccata cccaaccgta tgccgtctgc accccctttc
    10201 ccgaacccat gccgctgacc cgcctgttcc tgaaactcta cgccctgttg cgcctttttt
    10261 tgggcaaaaa cgcccagacc gcctggattt tccaccccgc ctgcaccggg cacgaccccg
    10321 gggcaaacca tcccgattcg cccgaccgca tcctctgcat cgagcaggca ttgcgccgcg
    10381 ccggtatttg gcagcacctc caaaccgtag aggcggaaga aatcagcgat acgcgccttg
    10441 cacttgtccg ctcgagcaaa tatctgaacc gtttggaatc ttgcctaccc gaagacggca
    10501 agatttgccg tttggacgac gacaccgtta tcagcaaaaa ctccctgtcc gccgcccgtt
    10561 tttccgccgg ttcggcagtt caggcagtcg atatggtcat gaaacgcaaa gcgtggcacg
    10621 ccttttgcgc cgcccgcccg ccggagcatc acgcgaaaag cggcaaagcc ggcggattct
    10681 gcctgctgaa caacgttgcc gccggcgtca tgcatgccat tgccgaatac cgcctgaaac
    10741 gcattgccgt catcgatttc gatgtccact acggcgacgg tacggcagaa atattcaaag
    10801 acgatccgcg tatcctgttt ttcaacctgt ttgaaaccga ccttttcccc ttccccgaaa
    10861 acaacggtat gcccgacggc ggcaatatgg tgcacctgcc cttgccgtcg ggaacgggca
    10921 gccgcacatt ccgcgaagcc gtccgcagac agtggctgcc ccggcttgcc gcattcaaac
    10981 ccgaactggt gctgctgtcg gcaggattcg acgcacaccg tctggacgaa tcgggcaggc
    11041 tcaacctgca cgaggcggat tttgcctggc tgacacacaa aatcattcag acggcatcca
    11101 gctgccccgg caaaatcgta tccgtactcg aaggcggcta cacccttgaa cctctggcac
    11161 aatctgctgc cgagcatatc cgcgtactgg cggggctggg caaatccgat gccgcaaccg
    11221 cctatcagaa aacactgaac ccaacaaaaa aacggtttgc caaaccgaaa accggacagg
    11281 tgcgccaacc tacccaatcc gaccgttacg atataaatgc cgtctgaagc cgattcggtt
    11341 ttcagacggc atcattattt gacctgacgc aaacagaaag ccttccgcgt gcttaaacac
    11401 atctcttgag acctttgcaa aaatccccaa aatcccctaa attcccacca aggcatttag
    11461 gggatttccc atga
//
LOCUS       DAXFWM010000074        10159 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000074 DAXFWM010000000
VERSION     DAXFWM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.074"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(74..985)
                     /gene="crgA"
                     /locus_tag="ACFI8B_001864"
     CDS             complement(74..985)
                     /gene="crgA"
                     /locus_tag="ACFI8B_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC3835014.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            1224..1802
                     /locus_tag="ACFI8B_001865"
     CDS             1224..1802
                     /locus_tag="ACFI8B_001865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC3835015.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(2010..3395)
                     /gene="pntB"
                     /locus_tag="ACFI8B_001866"
     CDS             complement(2010..3395)
                     /gene="pntB"
                     /locus_tag="ACFI8B_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC3835016.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(3475..3858)
                     /locus_tag="ACFI8B_001867"
     CDS             complement(3475..3858)
                     /locus_tag="ACFI8B_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC3835017.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(3862..5400)
                     /locus_tag="ACFI8B_001868"
     CDS             complement(3862..5400)
                     /locus_tag="ACFI8B_001868"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC3835018.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGGGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            5689..6522
                     /gene="serB"
                     /locus_tag="ACFI8B_001869"
     CDS             5689..6522
                     /gene="serB"
                     /locus_tag="ACFI8B_001869"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC3835019.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFEQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            6586..7808
                     /locus_tag="ACFI8B_001870"
                     /pseudo
     CDS             6586..7808
                     /locus_tag="ACFI8B_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            7969..9549
                     /gene="purH"
                     /locus_tag="ACFI8B_001871"
     CDS             7969..9549
                     /gene="purH"
                     /locus_tag="ACFI8B_001871"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC3835020.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQNLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAEYDGMISNYLTSLSDGVLSGEPEIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIHPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLAAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         2323 a         2708 c         2766 g         2362 t
ORIGIN      
        1 gtgtatcctt gagtaaggga gttgattgaa aagacttgtt tgattgatga tgaagaataa
       61 gttaattaat gtcttatttg gtattcgttc tattcatatt ttttcccagt tccttcacta
      121 aaaaatccaa aaatacgcgt aagcggaggt tgacggcttt atcgctgtaa taaacagcat
      181 taaaggggtg tgttttattg gaggtttgtt cggcgaatag gggaattaac tttccttcag
      241 tgatgtcgtt gtcaaccaaa aaatctgata agcaagcaat accgcaactt gaaaggcaca
      301 acgagcgtaa gatttcaccg ctgctggcgg taaagtgcgg tgaaatttta tagggatttc
      361 cctgcgcatc taaaaccgcc catgtattta gagaaccggg ttctgtgaag cctaaacatt
      421 gatggttggc aagatcttct gcagattgtg gcgtgccgtg ttttgctaaa tattcaggac
      481 tggcgactac gcggaagtgg ctgtcaaaca gatggcgtgc acgcagcccg gaatcgtcca
      541 attctccggc ccgtaaggca atatcgactt tgcgttcaat cagattgata tagccttcgg
      601 aagaaacgag cgaaagtcgg atatgcggat agcgttcgtt gaattttgct gccagcggcg
      661 ccagcagatg cagcaccatc ggcatcgcgg aatccacgcg caacacgcct tgcggtactt
      721 cgtgcactgc cagcatttcg gtttccgccg ctgccatttc ttgcaggatt ctctgcgcgc
      781 ggcggaaata ttgcgcgcct tcttccgtca gattgagttg ccgcgtggtg cggttgagca
      841 ggttcacgcc caacttttcc tccagccgtt tgacgatgcg gcttacggca gaatttgcca
      901 tctccaactg ctccgccgca cggctgaagc tgccgctttc caccacttga acaaatacgg
      961 tcagttcttc tgaattggtt ttcatcgtgt ttccttttcg gttggaaccc caccctttag
     1021 ggcggcagga tcagacttta tttgggaagg gcgtaacccc ttcccaatca ggacggcaca
     1081 tagggagacg atttatgtgt catcccgtgt gttgaaacat ctggttattc catttgacgc
     1141 aaaagtgttt tattattttt gcacttttaa atcataaagt aaaacggtac aatacattca
     1201 tcaattcaca aacgaggtaa caaatgaata ttttattatt agacggcggc aaagcgttcg
     1261 gacattctca cggcgggtta aaccacacgc ttcacaaaaa agcgaaagaa gttttgaccg
     1321 cgctcggaca caatgttcaa gaaaccgtga ttgatgccgg ctatgatgtt gaggcggaaa
     1381 tcgaaaaatt cgtttggatg gatgctgtga tttggcagat gccgggctgg tggatgcacg
     1441 agccttggac agtgaaaaaa tatatggacg aagtctttac cggcggacac ggcaaactct
     1501 accaaagcga cggcagacac cgcgtcaatc cgactgaggg ctacggcaca ggcggcttgt
     1561 tgcaaggcaa aaaacatatg ctttcactga cttggaatgc gccgattgaa gcctttaccc
     1621 gcgaaggcga tttctttgaa ggcaaaggcg ttgatgtttt gtatatgcac ttccacaaag
     1681 ccaacgagtt catcggcttg agccgcctgc cgacattctt atgtaacgat gtggttaaaa
     1741 atccgcaagt ggaaaaatac ttggcagatt atcaggcaca cttggaaaaa gtgttcggct
     1801 aattaaaaat ccatcttcaa cacggagatg gattttgttt gtttcgttga ttttgtgtca
     1861 gtttcagatg tgggtgctta tccggacggg tcgtctgaaa atgtttgccc caatgcaaaa
     1921 aaatcactgc aaaccttcac aaacggggct ggtagtgatt ttttcaaatc aaacagattg
     1981 aaaacctgcg ccgaattgtt cagacggcat tattttttca attccgccaa aatcccgtca
     2041 acggttttct tcgcgtcgcc gaaacacatc acgctgtttt cgttgaagaa cagcgggttt
     2101 tgtacgcctg cgtagccggt gttcatcgag cgtttgaata cgacgacttc ttttgccttc
     2161 catacttcta aaaccggcat acctgcaatc gggctgttcg gatcggtttg ggcggcaggg
     2221 ttgacggtat cgttcgcacc aatcaccaag accacatcgg tttcggggaa gtcgtcgttg
     2281 atttcgtcca tttccaaaac aatgtcgtag gggactttgg cttcggcgag cagtacattc
     2341 atatgaccgg gcaggcggcc ggcgacgggg tggatgccga agcgtacttc gatgccgttt
     2401 ttacgcaaaa gctcggtgat ttcggcaacg gggtattgtg cctgcgccac tgccatgccg
     2461 tagcccgggg tgataatgac gctgcttgcg cctttcagca tttcggcaac atcggcagct
     2521 ttgacttctc ggtattcctc tatctcttgg ctgccggaag acgatatgcc gctgtcgctg
     2581 ccgaaaccgc cggcaatcac ggaaacaaac gaccgattca tggctttgca cataatgtag
     2641 gacagaattg cgccgcttga gccgaccagc gcgccggtaa cgatgagcag gtcgttggag
     2701 agcataaagc ctgccgctgc ggccgcccag ccggagtagg agttgagcat agatacgacc
     2761 acgggcatat ccgcgccgcc gatggaggca accaagtgcc agccgaatgc gagggcgatc
     2821 agggtcataa tcagaaggat gaagccgctg ccgtcaatgc cgacaaatac gagcagcaac
     2881 acaaacgata cggcgagcgc cagtgcgttg agcttgtgtt tggcgggcag ttgcagcggg
     2941 ctgctgctga ttttgccgtt gagtttgccg aatgcggcca gtgagccggt aaaggttacc
     3001 gcgccgatga aaatgcccaa atagacttcg accagatgga tggtgtgcat atcgtgcgaa
     3061 acgttgcccg gctcgatgta gctgttgaag ccgaccaaaa cggctgccaa gccgacgaag
     3121 ctgtgcagaa gtgcaatcag ttcgggcatt tcggtcattt ctactttttt ggctttgtgg
     3181 atgccgattg ccgcgccgat gagcatggcg atgataatcc agcccagtcc gtgggtattg
     3241 tcggaaaaga ccgttacgaa gagggcgacc gccataccgg cgataccgga atagcagccc
     3301 cgtttggcgg tttcctgttt ggacagcccc gccagtgaga agatgaataa aattgcggca
     3361 acgatatacg ccgctgttac gagtcctgaa gacatggaaa ttctccgatt ttcgatgatt
     3421 tgttttcaat gccgtctgaa aaattgacgt tcgtgttttc agacggcatc tgtttcaagc
     3481 agccgcgaca aacagtccga cggtgcaggc aaatccgccc gcccacatca gcgagcgcaa
     3541 tgctgctttg tctgcgatgt agcaccagat aaaggcgagg cggaacagga tgaacaatcc
     3601 ggcaagcgtg ttgacggttg cttgtccggc attgccggtt gcgtgtgccg tcaaaacggc
     3661 ggcggcaaac ggtgcaaagg cttcaaaacc gttttgctgc gcggcgtggg cacgggcggc
     3721 tgcgccttgc gtatgtgcca gaaaaccgcg aggattgtgg ttgtctttaa accggaatcc
     3781 gcccgctttt ttggcatacg ccgcacaaaa aagcggcaat aggcaggcaa tcagaatgca
     3841 ccaataggcg aaagtcatgg cttacccttt cttaaacata ttcagcatac gccgtgtaac
     3901 cgcaaagccg ccgaagatat tgatgccggc aatcaggatg gcaacaaacg acagcagcga
     3961 aacgaagccg ttgccctgac cgatttgcag cagcgcgccg acgacgatga tgccggagat
     4021 ggcgttggtt accgacatca gcggtgtgtg cagcgagtgg ctgacgttcc aaacgacatg
     4081 gtagccgatg acgcaggcga ggacgaagac gataaagtgg ttcaagaatg ctgcgggtgc
     4141 gaccgcgccg acccacagca ccaatacggc ggcgatggcg gcgggcgcga gttttttcca
     4201 caggggaaca ggtttcggct cgggcttggc ggcaggcgcg gctttttcag acggcgtttg
     4261 ctgcggctgg gcggaaacct gaatcggcgg aggcgggaag gtgatttcgc cgtcgcgggt
     4321 aacggtcata ttgcggataa tcacgtcttc gaagtccagc gtgatttcgc cgtctttgtt
     4381 cgggcttaac agcttggtca ggttcaccaa gttggtggcg taaagctggg aagactgtcc
     4441 ggcaaggcgg tttgccatgt cggtgtagcc gatgattttc acgccgttgc cggttacgga
     4501 caattcgccc ggtcgggtga gttcgcagtt gccgcccgtc gccgccaaat cgacgatgac
     4561 ggatccggat ttcatgcttt ccaccatttc tttggtaatc agcttgggag cgggtttgcc
     4621 cggaatggcg gcggtggtga tgatgatgtc cacttctttc gcctgttcgg caaagagctt
     4681 catttcggcg gcgataaatt cgtcgctcat cactttggcg tagccgtctc cgccgccgcc
     4741 cgattcttgc aggaagtcga gtttcaggaa cttaccgccc atcgattcga tttgttccgc
     4801 cacttccaag cgggtatcga acgcgcgcac cactgcgccg agcgaatttg ccgtaccgat
     4861 tgccgccaaa cccgccacac ctgcaccaat caccaaaacc tgcgcaggcg gcactttgcc
     4921 ggcggcagtg atttgaccgg tgaagaaacg gccgaaggcg ttggcggctt caatcacggc
     4981 gcggtagccg ctgatgtttg ccattgaaga caaagcgtcc aaggcctgag cgcgggaaat
     5041 gcggggaacc atgtccatcg ccagcgcgtt gactttcttg gcgcgcaagg cctcgaccaa
     5101 agcctcgttt tggcgcggcc acaggaagct gacgatggtt tgaccttctt tgagcagcgg
     5161 cagctcgcct tcggacggcg cgttgacctt ataaattaaa gggcaggccc aaaccgccgc
     5221 tttgtcggca acggttgcgc ctgctgtttg gtaagcggca tcgtccaaac ttgccgccaa
     5281 acctgcaccg ctttcgacaa cggtttcaaa gcctagtttg cccagcaggg caacggtggc
     5341 gggcgtgcag gcgacgcggg tttcgccgga taatgactcg cgtgggatac cgattttcat
     5401 ctctgaatcc tttttcgggt tgttcatatg tatcgtgggt taaatttaaa tcggtgcagg
     5461 gcggagcaac gccgtaccgg tttaaaaccg attcacttca aatgttaata tattttagat
     5521 aatcccctta taacgaattt tcatcaggct ggcaatagtt gcgcattttc ccgtgttgtc
     5581 cgacacatac tgccgtttgc gtttgaagtc gttttccgat tgcaggcacg cccgccgtaa
     5641 cattctttgc cgtttcacta tataatccgc attttttgag ccgccgttat gccgcaagcc
     5701 ctcgtcctcc aatttccctc cgccgaagcc ctgccttccg acttcccctc acgcctgccc
     5761 gaacctgatt atgctgatga aaagcgtatg cgttttattg ttgaagaagg attttcttta
     5821 agcgaaaaag acgcggcgtt actggacagc cgtcaaatcg accacgccgt tttgcccaat
     5881 atggcgtttg gcgaactcgg gctgattgtc agcgatatgg attcgacgct gattaccatc
     5941 gaatgtatcg atgaaattgc ggcaggtgtg ggtttgaaag gcaaagtagc ggaaattacc
     6001 gagcgcgcca tgcgcggaga gttggatttc gaacagtctt tacgcagccg cgttgcgttg
     6061 ttggcgggat tggacgaaca gattttggcg gacatttatg aaaacgtttt gaagctctcg
     6121 cccggtgcgg aatttttgtt ggacgaatgc aaaaggcaca atgtgaaatt cctgctggtg
     6181 tccggcgggt tcacgttttt taccgaaagg ctgcaacaac ggctcggctt cgaataccaa
     6241 cacgccaata ttttggaaat tgaaaacggc aggttgaccg gccgtctgaa aggcagaatc
     6301 atcgacgcgc aggcaaaggc ggatttattg cgcgaatacc gcagccgcct cggattgcag
     6361 ccgcatcagg tgttggcgat gggcgacggt gcgaacgata ttccgatact caaagaagcg
     6421 ggcataggcg tggcttaccg cgccaaaccg aaagcgcggg ccgccgccga tgcctgtatc
     6481 aacttcggcg gtttggagcg tgtacgcggc ctgttcggat aggcggatag gaaacggatg
     6541 ccgtctgaaa ggttttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa
     6601 ctttggtttg ccctggcggc agcaggcgtt atcggcggtt tggtcggcat tgtgctgacg
     6661 gaactgatgc acttcatacg gcatacggca tacggttatg acgcggacgg tgcgtacatc
     6721 tcgttccgcg aaggtgtggc acaggctttc ggtatgcggc gcgttgccat gctgacgctg
     6781 tgcggcgcga tcgcaggcgg cggctggtgg ctgctcaaac gtttcggcaa gccgcaaatc
     6841 gaaatcaaag ccgccttgaa gcagccgttg caggggctgc cgtttctgac gacggttttc
     6901 catgttctgc tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcc
     6961 ccgcgtgaaa tgactgccgc gtttgctttt gccggcggca ggcggttggg tttggacgaa
     7021 ggcgaaatgc gcctcttgat tgcctgtgcg tccggagcgg gtttggcggc ggtgtataac
     7081 gtgccgctcg cctccacgct gtttatcctc gaagcgatgt tgggcgtgtg gacgcagcag
     7141 gcggttgccg ccgcattgct gacttccgtc atcgccaccg ccgtcgcgcg catcggctta
     7201 ggcgacgtgc agcagtatca tcccgccaac cttgccgtaa atacttcatt gctttggttt
     7261 tccgccgtca tcggcccgat acttggcgca accgccgtct ttttccggcg caccgcccaa
     7321 aaattcccct tcatcaagcg cgacaatatc aaaatcattc ccttggccgt ctgtatgttt
     7381 gtactcatcg gtgtgattgc cgtttggttt cccgaaattt tgggcaacgg caaagcaggc
     7441 aaccaactga cttttggcgg tttgaccggc tggcaataca gccttgagtt gaccgctgtc
     7501 aaatgggcgg tcgttttgat ggcgctggcg gcaggcgcat acggcggact gattacgccg
     7561 tctatgatgc tcggcagcac catttctttt gcagccgccg ccgcgtggaa cagcgtcttc
     7621 cccgaaatgc cgtctgaaag cgcggcagtc gtcggagcgg cggtgtttct cggcgtatcg
     7681 ctcaaaatgc cgctgaccgc cattgtgttc gtattggaac tgacctacgc gccgctgtcc
     7741 ctgctgctgc ctttgtgcat cggcatggcg gggcattggg aacatcggga aaaatgggct
     7801 tcaaatgaat gccgacaaat gccgtctgaa aggtttccag acggcatttc tttgcaaatt
     7861 gtcaacaata tctgcaaaaa cctactgcca aaaaactgaa aatagcggat aataccgccc
     7921 cgaaaaagat cccctttcac catccaaccg tcagaaaagg aagccccaat gtccgtcatc
     7981 aaacgcgccc tgatcagcct gtccgacaag gcaggcgcgg ttgaatttgc ccaaaacctg
     8041 cacaagctcg gtgtcgaaat cctctccacc ggcggcacgg cgaagctgct tgccggtgcg
     8101 ggcgttcccg tgattgaagt cgccgactat accggtttcc ccgaaatgct cgacggccgc
     8161 gtgaaaaccc tgcatcctaa aattcatggc ggcatcctcg gtcggcgcga tttggacgaa
     8221 cacgtcgcca agatggaaga acacggcatc ggcaacatcg acctcgtgtg cgtcaacctc
     8281 taccccttcg ccgccaccat cgccaagccc ggctgcacac tggaagacgc gattgaaaac
     8341 atcgacatcg gcggcccgac catggtgcgc tccgccgcga aaaactggaa acacgtcgcc
     8401 atcgttaccg acaccgccga cttccccgcc attgccgccg aactcgaagc cggcaacggc
     8461 gcgttgagcg ataaaacccg cttcaacctc tcacgcaaag cgttcagcca caccgccgaa
     8521 tacgacggca tgatttccaa ctatttaacc tcgctttcag acggtgtttt gagcggagag
     8581 cctgaaatcg gcgaatttcc gagccggttc aaccaaagct ggattaaagt gcaagatatg
     8641 cgctacggcg aaaaccctca ccaacgcgcc gcgttctacc gcgatattca tcctgcggca
     8701 ggcagccttt ccgcctacaa tcaactgcaa ggcaaagaat tgtcttacaa caacatcgcc
     8761 gatgccgatg ccgcttggga agccgtcaaa tccttcgacg cgcccgcctg cgtgatcgtg
     8821 aaacacgcca atccgtgcgg cgtagccgtt gcagccgata ccttggccgc ctacaaactc
     8881 gcctacgcca ccgacaccac cagcgcgttc ggcggcatca tcgccttcaa ccgcgaagtc
     8941 gacggcgaaa ccgtcaaaca gattaccgac aaccaattta tggaagtcct catggcgccg
     9001 aagtttaccg ccgaagccct tgaaatcgcc gccgccaaga aaaacgtgcg cgtattggaa
     9061 gtgccgctca aagcaggtgc caaccgcttt gaactcaaac gcgtcggcgg cggactgttg
     9121 gtacaaacgc ccgacatcaa ccgcatcaac cgcgccgatt tgaaagtcgt ctccaaacgc
     9181 caaccgaccg agcaggaatg gaacgatttg ctgtttgtct ggaacgtcgc aaaatacgtc
     9241 aaatccaacg ccatcgtctt cggcaaaggc ggccaaacct acggtatcgg cgcaggccaa
     9301 atgagccgcg tggacagcac ccgcatcgcc gcccgcaaag cgcaagatgc cggtctcgac
     9361 ctcaacggcg catgtgccgc ttccgatgcc ttcttcccat tccgcgacgg cgtggacgtg
     9421 attgccgaac agggcatcaa agccatcatc catccggcag gctcgatgcg cgatcaggaa
     9481 gttttcgacg cggcggacga acacggcatc gccatggcgg taaccggcat ccgccacttc
     9541 cgccattaag gcagacgaac aaggcaatgc cgtctgaagg gttttcagac ggcatttttg
     9601 cgctatttac cgtaatcctg aaatcccgtc attcccacgc aggcaggaat ctagatgaga
     9661 atccatcctg tcagaaatac ccgaatcgtc aggaataccc gtgtcatcat tcccgataca
     9721 ccgtaatcct gaaacccgtc attcccgcgc agacagaaat ccggatttgt ccgcgcggaa
     9781 acttatgcgc cgccattccc gcgaaggcgg gaatccggac cgttcggttt cggttatttc
     9841 cgataaattc ctgctgcttt tcattcctag attcccactt tcgcgggaat gacgaaggag
     9901 tgggaatccg gtttttgagt tccggccatt tccgacaaat tgctttggca ttggatattt
     9961 cctattttaa tccactatag tggattaact ttaaaccagt acggcgttgc ctcgccttag
    10021 ctcaaagaga acgattccct aaggtgctga agcaccaagt gaatcggttc cgtactattt
    10081 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccgccat atccgatcgc
    10141 cgcaccggaa ttttaaaaa
//
LOCUS       DAXFWM010000075        10015 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000075 DAXFWM010000000
VERSION     DAXFWM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.075"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(48..197)
                     /locus_tag="ACFI8B_001872"
     CDS             complement(48..197)
                     /locus_tag="ACFI8B_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835021.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADYREML
                     KRYAA"
     gene            complement(200..874)
                     /locus_tag="ACFI8B_001873"
     CDS             complement(200..874)
                     /locus_tag="ACFI8B_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835022.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(871..1356)
                     /locus_tag="ACFI8B_001874"
     CDS             complement(871..1356)
                     /locus_tag="ACFI8B_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835023.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(1362..1835)
                     /locus_tag="ACFI8B_001875"
     CDS             complement(1362..1835)
                     /locus_tag="ACFI8B_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835024.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(1890..3185)
                     /locus_tag="ACFI8B_001876"
     CDS             complement(1890..3185)
                     /locus_tag="ACFI8B_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC3835025.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELAHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(3811..>10015)
                     /locus_tag="ACFI8B_001877"
     CDS             complement(3811..>10015)
                     /locus_tag="ACFI8B_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC3835026.1"
                     /translation="PAPEGAQALRAAADGGAEQGGAGFDTAHHDQSHPALRQFADRTK
                     QEEAGRFFSGPADGNTPRAEELARGTEKQPDVSGIPSEDGAEFLDTGVMPDGLARQYA
                     DMAAEYRAKPSEAMGINPDDGAVSAAAALAADSGAAVPSAVSDDMEARSVADDVPSGR
                     SADADRGGVPSAYGNVRPGGAPRGAASVAPGGSAAAASGGIARVSPLPAGQYFDGLDT
                     RGRKALAKEAGLDIKGVADFGQIAAPVRRKIEQAYHARIEADYQAASEAKQGYLPPPV
                     RMADAVPVPKKGFSVPADALDKESRRRFDALPEWVRRHAQTVADYTADGIMRREAGMA
                     DMRGHYPEGLAESAGAVRAYRAQHPESADVLDRLNRAVYGYRRNNGWSVPLLSREGER
                     LQGVRTALPDDGASEAVVGGGRGLTRALPTEDKGLAQDVRQDVRQGLTQGGRGLTPDA
                     GADANAAALQGLPGSAVASGNAPARRQNLQVRARAEGAAPGLSASENLAGTDGGKRAP
                     VAGKRPDTVLPVLNPQVAASAGRVSPKKRMADAAADFTRRLAADRRRPEKAGVPLGGG
                     EYRFEHTDRRHIDALAGVPGRPGKGGMPEEFADMAGPSNSDGLVSDGRRYLKGREAET
                     LRAGGLSEAVPSEPGRDYRPTQEARAPARVMARPRDAAADGKPAGRAQPARAKDAPVA
                     GKAAAAKNAATEKPSSDKVRNIEAGKSHFDGGKGKSAAAQGAATEKPSSDKAAKPETF
                     AKTASDNPEEARRKARVLQGGPVYTVKERQAPQGFKALREHAENIKKRLAESIGGLAE
                     RVDVAAVSETAPDKAQMLLSQRVEGWFDGRTGKITLVAENLTPERAVWAAWHELGHRG
                     FAADGFAKYREELERADGNGLIRRIADAVQEGREGTGDAAASVRSAAVEEAVAELYAA
                     QRTGGWAGIENRYGVKVGNGLKRGIAGVLARIGALLRRVLQRLAGKAGGAMSDADVFA
                     MLADLHGNVEGARDAPWDGNHRAVMFARAEDGAAERSKSESLEKLRRAETIRISGREV
                     PEGGNLREYKRNALEYGKSLRGPYVNKDTGREISLGRAGIMEVLHHDVGNPEHLQSIA
                     AIPQIIEKSIYIDTLPNEDKAKNPDIREYEYYVAGLNIGGADYTVKAAIAVATTGDKY
                     YDHKLTGIEKGDLLEMTSRLSSAEISNQSPLSGIDDKRLLQILQDKDAGKGGIADFDT
                     EAVRFSRAANIGAAIGHITGKKSDLRNALKDRWDASKGIQLQFLGRRQIEDIYGGVLD
                     GLKEYGRLSELFGADANKAVTEADKVVREWGRLKEEDAKALADLMHDATLAKVDADPL
                     MRKDARGRLDGIRTALDIADGKIAKAEAAVASAGARIARADAAVRKAQRAADKAAYAL
                     EKAQEKHGREILADEADMRLRRLFYADSEAKRALRRAGADAAAESRAKTDAVRMSEQA
                     RADVKRLEKDEVGAQKALEGLALLNRRFAGLPDAAQRVYRKARDDYRAHFGQVRDALA
                     ERLARAGQDAETVRRLKERFDNELGGVYFPLARFGDYLVVVKDADGNSANVSRAETLS
                     EAEKLRDALKADFGPGFKVSPVMKSRDYIRSRDAVGSGFMKELGEAVGRMDLDPAQRA
                     RLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFSDDARRAYAQNMGSGANYLAKLRYAD
                     RMAEQLDVMQDFVDGRKYEEGFDQRQLQRVADEMRKRHEAVMNPNPSKLAQALTGFGF
                     LWMMGMSPASAVVNLSQTAMVAYPVMAAKWGYAGAARELLRASKQIGLRFGEKFNTIE
                     DSLNGDEKAAFRKAADYGVIDLSQAHDLAGVANGDPGLAGSAWQKVMDKAAWLFHHAE
                     KFNRQVTFVAAYRLAKRAGADSEAAFEQAKKATYDGHFDYAAQNRPRFMMGNAAKVVF
                     LFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHAMASGILGLPFVSTLLAVAS
                     MLGSDDDDPWDAEAALRNMLADTFGDKAGEVLAKGFSRLTPLDVSGRLGLDQLVFPDI
                     QDGLEGKKWAESLVVGSTGAVVGAGIGAADGVRTRSSVPRTANTLSLMKSW"
BASE COUNT         2018 a         3694 c         2381 g         1922 t
ORIGIN      
        1 cggtaaggta aacggaaaat gccgcccgaa tcttcggacg gcatttttca cgcggcatag
       61 cgtttcaaca tttcccgata atccgccggt tcccgtccgg cgaactcaaa ggccttcaaa
      121 tcggcgttat ctgatgcctt gcggctctcg ttcagccaat aggcgatctg tttcctgcaa
      181 aactcttcag cactcatcgt caaatccaaa aatgcctgtt caggtgcggc gcatcctcac
      241 gcgtgatacg gcgcaaatca ctgtcggccg gcaggccgaa atacagctcg aaacgcctcc
      301 ttgccgattc cgaacgctgc gggtcgaaag tctccgcatc cgggatactg aaagcctccg
      361 ccaaagccca ataaaccaaa ttccgatgat gtatggaatt gatttccggc tcgtccccgt
      421 cccccgccat atcccgcggc aggcaatacc ccccggcaaa caacctgccg tcacggtccg
      481 gcgcaggcgc aagcgtcagc ttcccgtcgc cctgcaccgc ataaaccggc agccccgttt
      541 gcgccccgtc gcgccaaccc ggggcgcaaa catccgccgc ctccggcgaa accaacttca
      601 ggcacaccgg cttgccgtcg gacacaaaac ggatactgtc gatttcatac gcataacccg
      661 ccgcaaaccc ccctgccgca taagcataaa gcggccgccc tttttccaca tccgttacaa
      721 acgcatcgtc cgaatgcagc agccggccgc gcaggcacgc ctcgtgaacc gcatcgttca
      781 accatcccga cacctcttcg tcgctccaaa aatacggctc ggccttatca ttggcaagca
      841 cgcggaaacg cgcaatcaaa gccttcaaat tcattgcgcg ataccgaact gacgaaccat
      901 ccgaaccacg tccccgcgca aattctccgc cgatttgcgc ttatccaaac cctggccgta
      961 attcgccttc gcatacagtt caagctcgtc cttgcccatc cggttgattt gcaggatcac
     1021 atcctgcacc gcgtcaaacg ccgccgtttc ccgttcgagt tccgaagcgc ccgccttttc
     1081 caaagcctcc gtatcatccg ccttttccac gcgtttgccc gggacggcgg caaacacatc
     1141 gccgtggcgc aacaactttg ccgcagcccc ggccgacacc tcgcgcacct ggccgtcttc
     1201 gaaaaccaag cccgtatgat agatacggtc gcgccacacg gcacgcccgc cgatatactt
     1261 gataaaaacc ttatccatcc aaccccccgc cgccgtccgg acggcattgt caaatacagc
     1321 aacgccgcaa aggcaaatcc ccgggcggcg tttcattccg cttacaactg gccgaacttc
     1381 tcgccgtaaa tcaaaatatc gatatcggcg gccttcttat tttccgcccc tttaagcgta
     1441 accgtcagca aagcctgctt gggcagcgta accagtttgg cagactggca gcggatacgc
     1501 ccggcagacg caaagtccgc gcccgaagca aaataagccg catcctgagg cactttcgca
     1561 tcatccgcac catccgcata cgcaaagccg acatcggcag tgataccgga agacatcgcc
     1621 gtcttcacaa taatctgcgc gtcatccaaa cggaaaccct gttccaagac acccagcgcc
     1681 aacacatcgc ccgccttagg cggctcagaa gcatccgccc ccaagatcgc accgtcctgc
     1741 ttcgcaacga tacggtaatg ctccgccgcc agattgccgt aaggcacgcc gccgaaacgg
     1801 ttgccgccat tgtttttcac ataaatctca gccataaaaa acctcaaccc aacaaatagg
     1861 aaaagccgcc ctttcaagcg gcattcgaat cagcggatac cgccgtgcaa aggaaccacc
     1921 gtatccacaa ccgtgacacc gtagtcggta atttccttcg cgcccccgcc gacatccacg
     1981 gcaaagcgcg ttttcgccac accgcgtatc gtaccgacga gcgtttccac acggttgccg
     2041 tgggcaagct ccttctcaga ccaaaagaaa ggcataccgc cgtgtttgcc ggtattcgca
     2101 aacgcctcca acaccgcctg gccgcccaaa ataaccgaac ggtcgaccgc aaacttgtcc
     2161 gcaaacgaag ccgggatttt caagcccgat tccgcctcgc tgaacttatc ggcgcaatac
     2221 ttcatctcat cgcccgcata gaaacggatg gggcgcggca ttttcaccag gataaagccg
     2281 ttccacaaac ccgcatcgcc caaaaacagc ggattttgat ttgcctggga ggcgcgcgcg
     2341 attgccgaag cctgaagctg gcggaatttc ggatcggcgg caaaacggtt gtactgcgcc
     2401 ggggaaagca gccacacgcg caaaggcgaa tcacccgccg ccttgtcgcc ctcaaacttc
     2461 acaatcggcg gcggcagcgg aatctggtcg agcacggtac gcatactgtc caccgtatcc
     2521 atcgtaaaca aatcggtaga ggcaatcttc aactcgccgc cgttgtcccc aacgcccgtt
     2581 accgcatcgc ccgaagccgt aaaatggcgg tttttggacg gggctttgac cggattgacc
     2641 gcataatcat tgaatttcgg gtcgcccgcc aaaggcacgc cccattcgat attgtcatga
     2701 aaaccgcgcg cgcccgccat atgcaccaac agcgtctggt cggcataacg gtccatcagg
     2761 ctttgcgccg ccggacgaat cagcgcgcga taatcggcag ggctgcgctg attcgtcatc
     2821 gtgccgccgc cgtcaacggg gaaacgcgcc tgattcacac gcaaacccgc ctccgacagg
     2881 ctcatcccca cgcccctgcc ttccgccgtg ttgtcgccca taatcggcag ggcggaaaca
     2941 gggttgacca aattgaaacg gatttcgtca cccatgccgc gcgtcaaatc ctgacagcgc
     3001 acgaccggca tatgctgggt cgtctgtttg cgcaaagtcg cctccgcacc cgcggtaccg
     3061 gcaggcatct tgcccgccaa acggttcagc gtactgttgc gctgcatatg catcgcaaac
     3121 agccccgccg cctgcttcat catcgcctgc gggtcgccgt aggccgtatt cgtcttttgt
     3181 gccataaaga gcctccctca aaaactcaag aatttaaaaa cttctcaacc tgctcttccg
     3241 tcatggaagc cagtttttcc gccagcgcgt tgccgctcaa ataatccggc gggccgtccg
     3301 aagccgtatg gtcgcgcccc gccggaatat ccgacagcgt attgggcgta ttcttcccgg
     3361 caggcggctt ttcggcgggc ttttccggtt cggcggcgga ttttgccgcc ttaaacatat
     3421 cgaacgcccc gatgacctcg gcggcagtcc cgtccttaag cgcgtcatta aacgccttgc
     3481 ggacaagcgg gttctgcgca tccagccact cctccagctc gcggctttcc acaatcgaat
     3541 ccgcatcggg gtgcgccgta tagatttcgc ggtaatgcgc ctttgccgcc tcctgccgct
     3601 tcatgtccgc ctcataaccc gccagcttct cctgaatcag cttttccacg ccctttttca
     3661 catcctcgtc ggaaaaatcc ccgaacaacg aattatcgga accgtcagcc gtttccactc
     3721 cgggtttttc cgcccccttc tcaagctggg caatgcgctc cctcaaggct tgggcttcgg
     3781 ctttcgccga cttttccgcc tcgcgcgcct ctaccagctt ttcataaggg atagtgtgtt
     3841 tgccgtcctt ggcacggatg accttgtccg cacgccgtcc gccgcgccga tacccgcgcc
     3901 gaccaccgcg cccgtactgc cgaccaccag cgattccgcc cacttcttac cctccaaacc
     3961 gtcttggata tcggggaaaa ccaactggtc caaacccaaa cgccccgaca cgtccagcgg
     4021 cgtcaggcgg ctgaagccct tggccaacac ctcgcccgcc ttatccccga aagtgtccgc
     4081 caacatattg cgcaacgccg cttccgcatc ccacgggtca tcgtcgtcac tgcccaacat
     4141 cgaagccacc gcaagcagcg tcgacacaaa cggcagcccc aagatgcccg aagccatcgc
     4201 atgcgagacc aacagccccg ccagcgtctt acgcgcctcc ttatccccct taaacgcaag
     4261 gtacgcattg cgccccagcg catacaggat attctgcgaa tactgcttga acaggaagac
     4321 caccttcgcc gcattgccca tcataaagcg cggacggttt tgcgccgcat agtcaaaatg
     4381 cccgtcatac gtcgcctttt tcgcctgttc gaaagccgcc tcactgtccg cccccgcccg
     4441 tttcgccaaa cggtaggccg cgacaaacgt aacctggcgg ttaaacttct ccgcatgatg
     4501 gaacagccag gccgccttat ccatcacctt ctgccaagcc gaccccgcca accccgggtc
     4561 gccgttggcc acccccgcca aatcatgcgc ctgcgacaaa tcgatcacac cgtaatccgc
     4621 cgcctttcgg aacgccgcct tctcatcccc gttcaaactg tcctcaatcg tattgaactt
     4681 ctccccgaac ctcagcccga tctgttttga agcccgcagc aattcccgcg ccgcaccggc
     4741 ataaccccac ttcgccgcca tcaccggata agccaccatt gccgtctgcg acaggttcac
     4801 aaccgcagaa gcgggcgaca tccccatcat ccacaaaaag ccgaagcccg tcaaagcctg
     4861 cgccagcttg gaaggattcg gattcatcac cgcctcgtgg cgtttcctca tctcatccgc
     4921 cacacgctgc aactgacgct ggtcgaaacc ctcctcatat ttgcgcccgt ccacaaaatc
     4981 ctgcatcaca tccaactgtt ccgccatacg gtccgcatag cgcaacttcg ccagataatt
     5041 cgcaccgctg cccatattct gcgcatatgc gcgcctcgca tcatcgctga agcccggcac
     5101 gcccttgcgg tggatgccgt gtttcgccca agacgtatcg ggcagggagt tcaaataaag
     5161 ctgcgtcagc gtatcgttca atcgggcccg ttgcgccgga tccaaatcca tcctgccgac
     5221 agcctcgccc agctccttca taaatccgct gccgaccgca tcgcggcttc ggatataatc
     5281 ccgggacttc atcacgggcg aaaccttaaa ccccggcccg aaatcagcct tcagcgcatc
     5341 gcgcagcttc tccgcctcgc tcaaagtttc cgcgcgggac acattcgcac tattcccgtc
     5401 cgcatccttg accaccacca gataatcgcc gaaacgcgcc aaagggaaat acacaccgcc
     5461 cagctcgttg tcaaaacgct ccttcaggcg gcgcaccgtt tccgcatcct gccccgcacg
     5521 cgccaaccgt tcggcaagcg catcgcgcac ctgcccgaaa tgcgccctat aatcatcccg
     5581 cgccttgcgg tacaccctct gcgccgcatc aggcagcccg gcaaaacggc ggttcagcaa
     5641 agcaagcccc tccaaagcct tttgcgcccc aacctcatcc ttttccaaac gcttcacatc
     5701 cgcgcgcgcc tgctccgaca tccgtaccgc atccgtttta gcccggcttt ccgccgccgc
     5761 atccgccccg gcgcgcctca acgcccgctt cgcctccgaa tccgcataaa acagacggcg
     5821 caggcgcata tccgcctcat ccgccaaaat ttcccgaccg tgtttttcct gcgccttctc
     5881 cagcgcataa gccgccttat ccgccgcccg ttgcgctttc cgtaccgcgg catccgcacg
     5941 cgcaatacgc gcaccggcgg aagcaacggc cgcctccgcc ttcgcgattt taccgtccgc
     6001 aatatccaaa gccgtccgga tgccgtccaa acgcccccgg gcatcctttc gcatcagcgg
     6061 gtcggcatcc accttcgcca gcgtcgcatc gtgcatcaga tccgcaagcg ctttcgcatc
     6121 ctcctccttc aacctgcccc attccctgac aaccttgtcc gcctccgtaa ccgccttgtt
     6181 cgcatccgcg ccgaagagtt ccgacaaacg cccgtattcc ttcaggccgt ccaaaacgcc
     6241 gccgtaaatg tcctcgatct gccgcctgcc caaaaactgg agctgaatcc ccttggaagc
     6301 atcccagcgg tctttcagcg cgtttctcaa atcggatttt ttacccgtta tatgaccgat
     6361 tgcggccccg atgttcgccg cacgggaaaa acgcaccgcc tccgtgtcaa aatcggcaat
     6421 gccgcccttg cccgcatctt tgtcttgcaa aatctgcaac aggcgtttat catcaatgcc
     6481 cgataaaggc gattgattgg aaatctccgc actggataag cgagaggtca tttccaaaag
     6541 gtcgcctttt tctattcccg tcaatttatg gtcatagtat ttgtcgcctg ttgtcgcaac
     6601 ggcgattgcc gcttttaccg tgtaatcagc cccgccgata ttcaagcctg caacataata
     6661 ttcatactct cgaatatcgg gatttttggc tttgtcttca ttcggcaaag tatcgatata
     6721 aatcgacttc tcgataatct gcgggatggc ggctatgctt tgcaaatgtt cagggttgcc
     6781 gacatcgtga tgcaacacct ccattatgcc cgcccgtccg agtgaaattt cacgccccgt
     6841 gtccttattc acataaggcc cgcgcaaaga tttgccgtat tccagcgcat tgcgcttata
     6901 ttcgcgcaaa ttgccgcctt ccggaacctc cctgcccgag atacggatgg tttccgcacg
     6961 gcgcagcttc tcaaggcttt ccgacttgga acgttccgcc gcaccgtctt cggcccgcgc
     7021 gaacatcact gcacgatgat tgccgtccca aggcgcatcc cgcgcccctt ccacattccc
     7081 gtgcaaatcc gccagcatcg caaacacatc cgcgtccgac atcgcaccgc cggccttgcc
     7141 cgccaggcgt tgcagcacac ggcgcaacag ggcgccgata cgcgccaaca cgcccgcaat
     7201 gccgcgtttc aaaccgttgc cgaccttcac gccgtaacgg ttttcaatgc ccgcccaacc
     7261 gccggtacgc tgcgccgcat aaagctccgc gaccgcctct tccaccgcgg cggagcgcac
     7321 cgaggccgcc gcatcgcccg tgccttcgcg cccttcctgc accgcgtccg caatgcgccg
     7381 aatcaggccg ttgccgtccg cacgttccaa ttcttcacgg tacttggcga aaccatccgc
     7441 cgcaaagccc ctgtgcccca gttcgtgcca cgccgcccat accgcacgtt cgggcgtaag
     7501 gttttccgcc accagcgtga ttttgcccgt cctgccgtca aaccagccct ccacacgctg
     7561 cgacaacagc atctgcgcct tgtccggcgc cgtttcggac acggcggcga catccacccg
     7621 ttccgccagt ccgcctatgc tttcggcaag gcgttttttg atattttcgg cgtgctcgcg
     7681 caatgcctta aaaccttgcg gcgcctgacg ctctttcacc gtataaacag gccctccctg
     7741 caacacacgc gccttgcgcc gtgcctcttc cggattgtcc gaagccgttt tcgcaaacgt
     7801 ttcaggcttg gcagccttat ccgaagacgg cttttcggtt gccgcgcctt gtgcggcggc
     7861 cgacttgccc tttccgccat cgaagtggga ttttcccgct tcgatatttc gcaccttatc
     7921 cgaagacggc ttttcagttg ccgcattttt tgcagcagcc gccttgcccg caacaggcgc
     7981 gtcttttgcc cgggcaggct gcgccctgcc cgccggtttg ccgtcggcgg cggcatcgcg
     8041 cggccgcgcc atcaccctgg ccggcgccct tgcttcctgc gtcggacgat aatcccgacc
     8101 cggctcggac ggcacggctt ccgacaaacc gcccgcccgc aaggtttccg cctcccgccc
     8161 cttcaaataa cggcggccgt cggagacaag gccgtcagag ttggaaggac cggccatatc
     8221 ggcaaactcc tccggcatcc cgcctttgcc cggcctgccc ggcacgcccg caagcgcatc
     8281 aatatgcctg cggtccgtat gctcgaaacg gtattcgccg ccccccaaag gcacacccgc
     8341 cttttccggc ctgcgcctgt ccgccgccaa acggcgcgtg aaatccgccg ccgcatccgc
     8401 catccgtttc ttaggcgata ccctgcccgc cgaagccgca acctgcggat tcaataccgg
     8461 caacaccgta tcggggcgtt tgcccgcaac aggcgcacgt ttcccgccgt ccgtcccggc
     8521 aaggttttca gacgcggaca ggccgggcgc ggcaccttcc gcgcgcgccc gaacctgtaa
     8581 gttttgccga cgggccggcg cattgccgga cgcaacggcg gaccctggca aaccctgcaa
     8641 ggcagccgca tttgcatccg cccccgcatc aggggtcaaa cccctgccgc cttgggtcaa
     8701 accctgccgc acatcctgcc gcacatcctg cgccaaaccc ttatcttccg tgggtaaagc
     8761 ccgggtcaaa cccctgccgc cgccgacaac ggcctcagac gcgccgtcat ccggcaacgc
     8821 cgtccgaacc ccctgcaaac gctccccctc gcggctcaac agcggcacgc tccaaccgtt
     8881 gttgcggcgg taaccgtaaa ccgcacggtt aagcctgtcc aacacatccg ccgattccgg
     8941 atgttgcgca cggtaagcgc gtacagcccc ggccgattcc gccaaaccct caggataatg
     9001 tccgcgcata tccgccatac ccgcctcccg gcgcataatc ccgtccgccg tatagtccgc
     9061 caccgtctgc gcatgacggc gcacccattc aggcagcgcg tcaaaccgcc tgcgcgattc
     9121 cttatccaac gcatcggcag ggacggaaaa ccctttttta ggaacaggca cggcatccgc
     9181 catacgcacg ggcggcggca ggtaaccctg tttggcttcg gaagccgcct gataatccgc
     9241 ctcaatccgc gcgtgatacg cctgctcgat ttttcggcgc acaggcgcgg cgatttgccc
     9301 gaaatccgca acgcccttaa tatcaaggcc cgcctccttc gccaaagcct tgcgcccccg
     9361 ggtatccaag ccgtcgaaat attggcccgc aggcagcggc gagacccgcg caatcccgcc
     9421 cgaagcggcg gcggcagaac cgcccggcgc aaccgaagcc gcaccgcgcg gcgcaccgcc
     9481 ggggcgcaca ttgccgtaag cggacggaac accgcccctg tccgcatccg ccgaccgtcc
     9541 ggacggcaca tcatccgcaa ccgaccgggc ttccatatcg tcagacaccg cagacggcac
     9601 agccgcgccc gaatccgccg ccaaagcagc ggccgcagaa accgcaccgt catccggatt
     9661 aatccccatc gcctccgacg gcttggcccg gtattcggcc gccatgtccg catactgacg
     9721 cgccaaaccg tccggcatca cgcccgtatc caaaaattcc gccccgtcct ccgacgggat
     9781 acccgaaaca tccggctgtt tttccgtccc gcgcgccaat tcctccgcgc ggggcgtatt
     9841 gccgtcggca gggccgctga aaaaccgccc cgcctcctcc tgcttcgtac ggtccgcaaa
     9901 ctgccgcaaa gccggatgcg actgatcgtg atgcgccgta tcaaaacccg cgccgccctg
     9961 ttccgcgccg ccgtccgccg ccgcacgcaa cgcctgcgcc ccttccgggg ccggc
//
LOCUS       DAXFWM010000076         9729 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000076 DAXFWM010000000
VERSION     DAXFWM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.076"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(61..258)
                     /locus_tag="ACFI8B_001878"
     CDS             complement(61..258)
                     /locus_tag="ACFI8B_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835027.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(263..553)
                     /locus_tag="ACFI8B_001879"
     CDS             complement(263..553)
                     /locus_tag="ACFI8B_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835028.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            complement(598..1946)
                     /locus_tag="ACFI8B_001880"
                     /pseudo
     CDS             complement(598..1946)
                     /locus_tag="ACFI8B_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            2618..3022
                     /locus_tag="ACFI8B_001881"
     CDS             2618..3022
                     /locus_tag="ACFI8B_001881"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC3835029.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            3062..4243
                     /locus_tag="ACFI8B_001882"
     CDS             3062..4243
                     /locus_tag="ACFI8B_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC3835030.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVTLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(4346..6601)
                     /locus_tag="ACFI8B_001883"
     CDS             complement(4346..6601)
                     /locus_tag="ACFI8B_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC3835031.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            6974..8152
                     /gene="nirK"
                     /locus_tag="ACFI8B_001884"
     CDS             6974..8152
                     /gene="nirK"
                     /locus_tag="ACFI8B_001884"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC3835032.1"
                     /translation="MKRQALAAMIASLFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            8330..9085
                     /locus_tag="ACFI8B_001885"
     CDS             8330..9085
                     /locus_tag="ACFI8B_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC3835033.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            9300..9729
                     /locus_tag="ACFI8B_001886"
                     /pseudo
     CDS             9300..9729
                     /locus_tag="ACFI8B_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT         2442 a         2642 c         2398 g         2247 t
ORIGIN      
        1 aaaaagtgtt tggcggtaat ggatggagag tttttcagac gaccgccgaa catccgaaaa
       61 tcagtctttc aaaaatccga atacgacaaa ttcgcattgg ttgccgattt cttccaaacc
      121 tgcgttaacc gcttcttcga agtcgtagaa ataatcggca ttggtaatta atttggtatg
      181 tccgatgtcg cccgtttcag gagagagata cagaaagtcc cctgttgata cggactggac
      241 aacatagact ttctgcattc aatcagcctt tcttaacgaa ttgaaaaccg gtgactttca
      301 gtttttgagt tttgcccgta gtgacgattt ccacgttcag gtttgcttcg atcggaaatt
      361 gggcgtttcg gaactgctcg aaattggcag agccgccgaa atcgtattca gtagtagagc
      421 tgcccaatgc gttgccttgg gagctgtcta agggtgtggc gacaatcagg cggcaatagt
      481 cgaagctctt gccttcgatt tgtccgttga attttttaac gccgacgatg tggccttgaa
      541 gttggatgtt cattttttgg tttccttgtg tgattaaacg tctttcgggc agacacttta
      601 agcccatgaa atcggtagtc ttgcgaattt gtcgtaaatg aagttgttat agctttcttc
      661 attgttgacg tgtttttgct gttcaagctg tttttcaaga ttctcgtaat attcgtacat
      721 ataataaggg tcattgtacg gtttgaatgc gggctgttca tgaatgactt gagatttcaa
      781 aaaggcgcag tcgtaggctt cgagagccaa agacttgggc agcttgtgat gactcggctc
      841 aatcagttca aacagtttgg ctttgtccaa ttcgggaaaa atgaatttca gaccgtttgc
      901 cgcacgtccg aactgttttt ttacccattc aaggcagcgg tcggctgaaa caaccttatc
      961 ttccttaacc gcgtgtatgc gcgttgcctt ttgggcgaat cgttcgcaaa tgggatatgc
     1021 gccgccgaaa tattcgcccg gattctgcaa aacttcgaaa gggataacga tgtcttttgc
     1081 tttgaattca atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt
     1141 ttcatagacg cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt
     1201 ggtcattttg gtttcgtcgt cttcttccca atccgacccc aaacattcgc ccttcggttt
     1261 gacgtgatgg cacgtaaaca aacctttatt gcggtcttct cttgcctgat tcgggctgta
     1321 ctctccgttg aaaaagtctt ttgcaatgtc aacgcgcgtt atttttggac gaatcgcatt
     1381 tgtcagaaaa tcaaaaagcc ttgattccca tccaatattt gcagcattgc agcctaccgc
     1441 cgtcaattca accaacatcg tttcacgttg gccgccgtaa tggacgcgcc cgtattgggc
     1501 attttccgtt cccatcagcc agcagccttc atagaaacgc ccgcccgaat gcttggcttt
     1561 ttcaatgatt ccgaatccga aaatttcctc catcttggcg gaagccgcgc gtatgaaatc
     1621 gtcatcatcg aacaaatcaa acgaaagtcc ataaacatgg aaaaacgtgt cttcatgaat
     1681 tgaaaatgtg atttggtcaa tgaaagccga atctgataca ccgcgcctaa gaggaacgcc
     1741 taacaggttt cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac
     1801 cctgcccgcc gtttcggttt ctgtaccccc cctgttagat aagggggggg gggaagattc
     1861 gaagcggttg ccggcttcct gccgtccgcc ggcgcctccg tcatcacgcc ggcaaccgcc
     1921 tttgtcatcc cttgcttatc tcccatttgc gaatcctcaa aaacgggcaa aaaaaaagcc
     1981 ctgttacttg tagaaagtaa agggcgttaa tttttgttaa tcaccccttc ttagggacgc
     2041 aatatataag gtttcagacg gcattgttct cccgccgtca ttcccgcgca ggcgggaatc
     2101 cggaacgtaa aatctaaaga aaccgtttta tccgataagt ttccgtgccg acaggcccgg
     2161 attcccgcct gcgcgggaat gacgggattt taggtttctg attttggttt tctgtttttg
     2221 aggaaatgac gggattttag taaccgtaac aactgcctgc gcgacggcta aggggcttca
     2281 gcaaccgtag caactgcctg tgtgggaatg acggacaatg ggcttcagac ggcatctctt
     2341 gccttccgct aaaacagttt gccgcacaac tgttcaaacg cgtccgatat gtttcaacac
     2401 acaggacgac acataaagca ccgccctata tgtcgtcctg attcggaagg ggttacgccc
     2461 ctcccaaaca aagtctgatc ctgccgccct aaagggcggg gtttcaaccg aaaaggaaac
     2521 acgatgaagt ggtacaatta acggcaatgc ggacagacaa attaaactat cagaaattgc
     2581 cctaccgttt tttaaacact ttcaggaata aggaaaaatg accgcccaac cctgccccat
     2641 ctgcacggca caagatgaag atattttgct gcaaaccccc aagctgcgcg tcatcgccgt
     2701 ccataacgac agcggttcgc cggcattctg ccgcgtcatt tggcacggac acattgccga
     2761 aattaccgac ctttcggcag cggaacgcgg cgaattgatg gaaatggtgt acaaagtcga
     2821 agccgctatg cgccaagtgt tccggccggc aaaaatcaac ctcgccagct tgggcaatgt
     2881 cgtgccgcac ctgcattggc atattatcgc ccgctttgaa aacgatgcga ctttccctgc
     2941 gccgatttgg gcaaaccccg tccgaaaaca cggtatgacc ctgccgcaaa attggacgga
     3001 acagcttaaa aagctgcttt aaacccgccg atgccgtctg aaaccgtatg aaagggaaat
     3061 tatgaccgaa ccgacctccc gccgccgttt tctgaaaacc tgcaccgccg ccggcgcggg
     3121 gctgcttcag gcttgcggca catccgccac atccgttccg ccccttccct cttcccattc
     3181 cgttgtgaaa gcccgaaccg tgactctcca aacgccacgc cgtcaaagtt cggacggcaa
     3241 ccttctgcgc gttgtcgctt cgtcaggatt tgccgaagac accaaccgcg tcaacacagc
     3301 cttaacccgc ctttacaatg ccggttttac cgtaaccaac caacaggcgg gcagccgccg
     3361 tttccaacgg tttgccggca cggacgcgca acgcgcggca gatttccaag aggtcgcctc
     3421 cggccgcgtc gccacgccta aagtgctgat gggcttgcgc ggcggttacg gtgcggcgcg
     3481 gattctgccg catatcgatt ttgcctcact cggcgcaagg atgcgcgaac acggcacgct
     3541 gtttttcgga ttcagcgacg tatgcgccgt ccaactggca ttgttggcaa aaggcaatat
     3601 gatgagtttt gccggcccga tggcttatag cgattttggc aaacctgccc ccggtgcgtt
     3661 tacgatggat gcctttatca agggggcaac ccaaaaccgc ctgaccgttg atgttcctta
     3721 tatccaacgc gccgatgtcg aaaccgaagg cacgttgtgg ggcggcaact taagcgtcct
     3781 cgcctcgctc gccggtacgc cttatatgcc cgacatcgac ggcggcattt tgttcctcga
     3841 agatgtcggc gaacagccct accgcatcga acgtatgctc aatacgctgt atctttcggg
     3901 tattttgggc aaacagcgcg ccatcgtgtt cggcgatttc cgtatggaaa aaattcgaga
     3961 tctctatgat tcgtcttatg atttttccgc cgttgccaag catatttcgc gcacggcgaa
     4021 aatccccgtg ctgacgggct tcccgttcgg acacattgcc gacaaaatca ctttccctct
     4081 gggcgcgcac acccgaatcc gtatgaacgg aaacggcggc tattcggtcg cgtttgaagg
     4141 ctaccccaca ctcgatgcgt ccgccctgac tttggatacc ctgctcccac cgccggattt
     4201 gcctatcttc cccgaaagcg gtgttgccga tatttcggaa taaacccgca aacggacaaa
     4261 tgccgtctga agccttcaga cggcattttc ctgtaaaaaa aaccgcacct gatggcaggt
     4321 gcggtttcca ttttcaggct aaaacttatt tgtcgcggcc gaatacgatt ttagtggctt
     4381 ggatggcaac gcagattgca ccgccgataa agattaagtc gggggcagtg cgtacccagc
     4441 gcagggtgtc gaggatttcc atttgcagga attcttcgct gcgggcatac cacaaaccgt
     4501 gcgtgataga ggcgtatgcc tgaatcacgc cgacaggcag caggctgatg gcaatcatac
     4561 cgaccaagcc gccgttgagc agccagaagc cccaagtcat cagtttgtcg tcaaaccgcg
     4621 cgttcggttt caagtagcgc gcaaccaaca atacgaagcc caatgccaag aaaccgtaca
     4681 caccgaacaa ggcggcgtgc gcgtgaacgg cggaagtgtt caaaccttgg atatagaaca
     4741 gggaaatcgg cggattgatc agaaagccga atacgccggc accgatcata ttccaaaaag
     4801 cgactgccac gaagcacatc agcggccaac gcaggcgttt cgcccagtcg gacaggtgtt
     4861 ggtaagacca gtgctcgtat gcctcgcggc ccagcaacac cagcggcacg acttccaaag
     4921 cggagaagca ggcgccgatt gccatagagg cggaggtaga gccggagaag tacaggtggt
     4981 gcagcgtgcc cggaacgccg cccaacataa agatggcggc agcggccaaa gtggaggcag
     5041 tggcggtact gcggcggaca aagcccatat tgtagaagac aaaggcaaag gcggcagtgg
     5101 caaatacttc aaagaagcct tccacccaca ggtggaccac ccaccaacgc cagtattcca
     5161 ttacggcaat cggggatttt tcgccataga acaggcccgg tgcgtagaac acgcccacgc
     5221 cgaccataga agcgacaaag attgccagca agtttttgtc cacgcctttt tctttgaagg
     5281 cggaaaccgt gcagcgcaac atcaggaaca gccacaacag cagtccgacc atcaaaagga
     5341 gttgccagaa acgtcccaaa tcgaggtatt cgtaaccttg gtgtccgaac cagaagttaa
     5401 attcgggagg aaggatgtgc gtcaacgcga agaagttgcc cgcgtaagaa ccgccgacca
     5461 cgatgaagag ggcgatatag aggaagttca cgcctgcacg ttggaacttg ggatctttgc
     5521 cgccgttgac aatcggcgcg aggaacaaac ctgccgtcaa aaagccggtt gcaatccaga
     5581 agatggcgga ttggatgtgc caagtacggg tcagggcgta gggaaaccag tcggacattt
     5641 caaagcccaa cgcctcgtca atgccgtaga aaccctgacc ttcgacggtg tagtgcgcgg
     5701 tcaggccgcc cagcaatact tgtaccacaa acagggcgac cgtcaggaag acgtatttgc
     5761 ccaatgcttt ttgcgaaggg gtcagttgga ttttggaaat cgggtcttca gacggcactt
     5821 ccacttcctc gtgtttggtc aggaaggaat aaccccacat caacaaaccg atgcccatca
     5881 agagcagtac gacactggta aacgaccaca tgtagttttc agtagtcggt acattgttga
     5941 tcaaaggctc gtgcggccag ttgttggtgt aggtaaagac ctcgccggga cggttggtcg
     6001 aagcagacca agaagtccag aagaagaagt cgaacagttt ttcacgcgct tcttggcttg
     6061 gcaatgtgtt gtttttcatt gcaaagtgtt cgcgggtggt ttgcaacttg ggatcatcgc
     6121 cgtaaacgcc gtgatagtaa ggcaggatgc tttcgatggc tttcacgcgc gtatcgctga
     6181 tgacgacgct gccgtcttct ttaatacggc tttgattgcg gtattcatcg gccaagcggg
     6241 ttttcagaac ggcttgttct tcaggggaaa cttcatcgaa ttttttgccg taagcctgtt
     6301 gcgcggtcaa atccaaccag gcggacaact cacgatgcag ccagtcggcc gtccaatccg
     6361 gagcctgata tgcgccgtgt cccagaatcg aaccgacttc catgccgccg gtagtctgcc
     6421 acgcagactg acctgccaaa atatcgtctt tcgtcatcag cactttgccg gatgcggaaa
     6481 cgacctgttc ggggtaaggc ggggctttct tataaacctc gctgcccata tagccaagaa
     6541 tggtaaagca taccgccaga acggcaaaca gcaagtacca cagcttcttg tactgtccca
     6601 ttttgagagc tccttttaat aaatcagttg tttaaaattc acaaaatatg aatgttaaag
     6661 attgtagcat ggtttaccgc gcaaataaac atttgttcaa acaaactcac atatagaaca
     6721 aatacatata tgataataac tatcattatt ctttactcgg aaattgccct gcctttgcct
     6781 gctctgccgg agcccctagc aaatcagcct attcattgta atttttagta gttataaagt
     6841 attagaagcg tcattttaag ttcatatttt atgaattatt tgacttaaat taaaatgccc
     6901 gcaatgggac aaccgcataa tcacaccaag ttcttaacta atccccctac ttttcttaca
     6961 aaaggaaaac attatgaaac gccaagcatt agccgcaatg attgcttcct tattcgcatt
     7021 ggccgcctgc ggtggcgaac aagctgctca agcccctgcc gaaacccctg ccgcttccgc
     7081 agaagccgca agttccgccg cacaagctac cgccgaaacg cctgcaggcg aactgcccgt
     7141 catcgatgcg gtgaccaccc acgctcccga agtacctccc gcaatcgacc gcgactatcc
     7201 tgccaaagta cgcgtaaaaa tggaaaccgt cgaaaaaacc atgaaaatgg acgacggggt
     7261 ggaataccgc tactggacat ttgacggcga cgttccgggc cgtatgatcc gcgtacgcga
     7321 aggcgatacg gttgaagtcg aattctccaa caatccttct tctaccgttc cgcacaacgt
     7381 cgacttccac gccgcaaccg gtcagggcgg cggtgcagcc gcgaccttta ccgccccggg
     7441 ccgcacttcc acattcagct tcaaagccct gcaaccgggc ctgtacatct accactgcgc
     7501 cgtcgcgccg gtcggtatgc acatcgccaa cggtatgtac ggtctgattt tggtcgagcc
     7561 taaagaaggc ctgccgaaag tggataaaga gttttacatc gtccaaggcg acttctacac
     7621 caaaggcaaa aaaggcgcgc aaggcctgca accgttcgat atggacaaag ccgttgccga
     7681 acagcctgaa tacgtcgtat tcaacggcca cgtaggcgct atcgccggcg acaacgccct
     7741 gaaagcgaaa gcaggcgaaa ccgtgcgtat gtacgtcggt aacggcggcc cgaacttggt
     7801 gtcttccttc cacgtcatcg gcgaaatctt cgacaaagtt tatgttgaag gcggcaaact
     7861 gattaacgaa aacgtacaaa gcaccatcgt gcctgccggc ggttctgcca tcgtcgaatt
     7921 caaagtcgac atcccgggca gctacacttt ggtcgaccac tccatcttcc gcgcattcaa
     7981 caaaggcgcg ttggggcaat tgaaagtaga gggtgcggaa aaccctgaaa tcatgactca
     8041 aaaattgagt gataccgctt acgccggcag cggcgcggct tctgcccctg ctgcttccgc
     8101 accggctgct tctgccccgg cagcctctgc atccgaaaaa agcgtttatt aaatcggata
     8161 cccgtcatta gcgggacaaa ccactgccgc cgtacttcat tacgcacggc agtggttttt
     8221 taacaaccaa tctttccttt cggaagattg atttttcaga cggcattcgc ggctttttgt
     8281 ttaaaatgcc gtctgaaaaa tcaatgctaa ccgcctgtca ggaggcttta tgaagtacgt
     8341 ccggttattt ttcctcggca cggcactcgc cggcactcaa gcggcggctg ccgaaatggt
     8401 tcaaatcgaa ggcggcagct accgcccgct ttatctgaaa aaagataccg gcctgattaa
     8461 agtcaaaccg ttcaaactgg ataaatatcc cgttaccaat gccgagtttg ccgaatttgt
     8521 caacagccac ccccaatggc aaaaaggcag gatcggttcc aaacaggcag aacccgctta
     8581 cctgaagcat tggatgaaaa acggcagccg cagctatgcg ccgaaggcgg gcgaattaaa
     8641 acaaccggta accaatgttt cctggtttgc cgccaacgcc tattgcgccg cacaaggcaa
     8701 acgcctgccg accatcgacg aatgggaatt tgccggactt gcttccgcca cgcagaaaaa
     8761 cggctcaaac gaacccggct acaaccgcac tattctcgat tggtatgccg acggcggacg
     8821 gaaaggcctg cacgatgtcg gcaaaggccg cccgaactac tggggtgttt atgatatgca
     8881 cgggctgatt tgggaatgga cggaagattt caacagcagc ctgctttctt ccggcaatgc
     8941 caacgcgcaa atgttttgca gcggcgcatc tgtcggggcg agcgactcgt ccaactatgc
     9001 cgccttcctc cgctacggca tccgcaccag cctgcaatcc aaatatgtcc tgcacaactt
     9061 gggcttccgc tgcgcaagcc gataacccct taaattatag tggattaaca aaaaccagta
     9121 cggcgttgcc tcgccttgcc gtactggttt ttgttaatcc gctatattcc gccatctcta
     9181 agatttacag cgatacacgg gtaatttaag gaatgcccga accgtcattc ccgcaacttt
     9241 tcgtcattcc cgcgaaagcg ggaatctgga atctcggact ttcagataat ctttgaatat
     9301 tgccgttgtt ccaaggtccg gactcccgcc cgcgcgggaa tgacgaagcc atccttttgg
     9361 ccgaaggtca aaaatcagcc gttaccgagt attacctgaa taacggcgaa tggccgaaag
     9421 acaacggcga tgccggcgtg gcatccgctt cagaaatcaa aggcaaatat gttcaaagcg
     9481 ttacggtcgc aaacggcgtc gttaccgccg aaatgaaatc agacggcgta aacaaagaaa
     9541 tcaaaggcaa aaaactctcc ctgtggggca ggcgtcaaga cggttcggta aaatggttct
     9601 gcggacagcc ggttaagcgc gacgacgccg ccgacaacga cgacgccgtc accgacgaca
     9661 ccaccggcaa cgccatcgaa accaagcacc tgccgtcaac ctgccgcgat aaggcatctg
     9721 atgccaaat
//
LOCUS       DAXFWM010000077         9371 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000077 DAXFWM010000000
VERSION     DAXFWM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.077"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(102..2951)
                     /gene="gcvP"
                     /locus_tag="ACFI8B_001887"
     CDS             complement(102..2951)
                     /gene="gcvP"
                     /locus_tag="ACFI8B_001887"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC3835034.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVDTDEHGNVNIDDL
                     KAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIMQ
                     PAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQTS
                     VAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKNG
                     RIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKAE
                     LDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPLP
                     FVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2982..3341)
                     /locus_tag="ACFI8B_001888"
     CDS             complement(2982..3341)
                     /locus_tag="ACFI8B_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835035.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3340..3519
                     /locus_tag="ACFI8B_001889"
     CDS             3340..3519
                     /locus_tag="ACFI8B_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC3835036.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3512..4153)
                     /locus_tag="ACFI8B_001890"
     CDS             complement(3512..4153)
                     /locus_tag="ACFI8B_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC3835037.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4143..4694)
                     /locus_tag="ACFI8B_001891"
     CDS             complement(4143..4694)
                     /locus_tag="ACFI8B_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC3835038.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4698..5216)
                     /locus_tag="ACFI8B_001892"
     CDS             complement(4698..5216)
                     /locus_tag="ACFI8B_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC3835039.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5216..6877)
                     /gene="pqiB"
                     /locus_tag="ACFI8B_001893"
     CDS             complement(5216..6877)
                     /gene="pqiB"
                     /locus_tag="ACFI8B_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC3835040.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQAKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6870..8289)
                     /locus_tag="ACFI8B_001894"
                     /pseudo
     CDS             complement(6870..8289)
                     /locus_tag="ACFI8B_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
     gene            8516..9145
                     /locus_tag="ACFI8B_001895"
     CDS             8516..9145
                     /locus_tag="ACFI8B_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC3835041.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
     gene            complement(9170..>9371)
                     /locus_tag="ACFI8B_001896"
     CDS             complement(9170..>9371)
                     /locus_tag="ACFI8B_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3835042.1"
                     /translation="RSKEFADRRNHINGIGNFWNQAKRALRKYNGIDRKPFPPLLREC
                     EFRLNSGTPSRQLKILRDRCGI"
BASE COUNT         2087 a         2418 c         2670 g         2196 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacttt caaaccgagc
     1141 atggcggcgg tggcggggtt ggtgccgtgg gcggatttgg gaatcaggca gatgttgcgg
     1201 tgtgcttcgc cttgggcttc ctgataacgg cggatggcga gcataccgct gtattcgcct
     1261 tgtgcgccgg agttgggctg gaaggaaatc gcgtcaaagc cggtgatggc tttcaggctg
     1321 ttttccatat cagcaagcaa ttcgcggtag ccggcggttt gcgcttcggg agcgtaaggg
     1381 tggatgtcgg agaactcagt ccaagtaatc ggcaacattt ccgcagtcgc attgagcttc
     1441 atggtgcagc tcccgagcga aatcatgctg cggttcatag ccaagtcgcg gtcttcgagt
     1501 tttttcaggt agcgcaacat ttcgtgttcg gtgtggtaac tgttgtacac aggatgttgc
     1561 agaatgtcgt cttgacgcag taattcggcg ttcagacggc ctttgacatc atcggcaaat
     1621 gtgaacgtat ccttgccggt aaacgcgcgg tacaaatcgg ccaaatcttc gtataccgac
     1681 gtttcatgga atgcagccgc aacttgagta ttgttgacgc ggcgcaggtt gtaacccgac
     1741 tccaaagcag cggcaaacac ttggtctgct ttctctttgc tgccgaaatc gacggtaacg
     1801 gtatcgaaga agacttcgtg aaccactttg aggccgtctg aaaccagcgc gtcggcaaag
     1861 acggaagcca gtgtgtgaat gcggttggcg atgcgtttca cgcctttggg gccgtgataa
     1921 acggcgtaca tgcccgccaa gttcgccagc aatgcctgtg cggtacaaat attggatgtc
     1981 gctttttcgc ggcggatgtg ttgttcgcgg gtggacaaag ccatgcgcaa ggcaggtttg
     2041 cccgatgcgt ctttggatac gccgatgatg cggcccgggg cggagcgttt gaactcgtct
     2101 ttaaacgcga aataagcggc gtgcggacca ccaaagccca tcggtacgcc gaaacgttgg
     2161 gtgttgccca aagcaatatc tgcacccaat tcagccggag acttcagcaa aaccaagctc
     2221 atgatgtcgg cggcaacagc aacgattgtg cctttggctt tcagacggcc gataacgtcc
     2281 tgcaagtctt gcacgtcgcc atctttaccg acgtattgga acagcgcgcc gaagtattcg
     2341 ccttcatccg cttgggcaaa atcgctgacc accagctcga agccgaaata tttggcgcgg
     2401 gttttcatca cgtccaaagt ttgcggatac acgcgcgcat ccacaaagaa acgctcggat
     2461 ttcactttgc ccacgcggtg cgccatcgcc atcgcttcgg cggcggcggt cgcttcgtcc
     2521 aacaaagacg cgcccgccac agggaaaccg gtcaaatcga tgcacacctg ctggaagttc
     2581 aacaacgctt ccaaacgacc ttgggcaatc tccgcctgat acggcgtgta ggcggtgtac
     2641 cagcccgggt tttccaatac gttgcgcaaa atcacgttcg gcacgcgggt cgggtaataa
     2701 cccaagccga tataggactt gttgatcacg tttttcgacg cgatgccttt caatttcgcc
     2761 aatgcgtccg cctcggtcag ggcttcgggc aaatcgagtt cggacggcat acggatgctt
     2821 tgcggcaagg tgttgccgac aaagtcgtcc atgctcttct cgccgacagc ggcaagcaac
     2881 gccgcttcgt cgccgaaact caaatggcgc gcggcaaatt cgttggggtt gaacagttcg
     2941 gacagtttca tttctactcc ttcgggtttg tagggctgcc atcaggcaga atttttagac
     3001 atcggatgat tcgggcaagg gtggcacaaa aggcgggata atggcggaca acgcgccaga
     3061 atgcagcagg tcgggacaag cagcgccaca agcggcagcg ataagggaaa gccgccgccc
     3121 aaaaccatga aacctgcagg caccagccaa gccaagggat gagccggaag cggaaaaaca
     3181 acattgagca gaaccaaaat cacgacaaac agcggcagcc ccgccatcag gttggcacgc
     3241 ctgtatgttg cgtacgggtc ggatgcttct tcaggttcgc aatattccat gatttcagcc
     3301 tgtccgggct ttatccgcag caggttcgga cggtcggtca tgccgtctga accggcaggc
     3361 cgtctttctc aaaaaacgcc ataccgctgc aacttcatgc ggctgtatgg cgtaggtttt
     3421 gcacttcaga cggcatatgc gtcccgaagc agtttggtgt cccagtcggt tcatgattat
     3481 ataaacaact tgggtgcgga taaacctttg tttatctaaa cccatgtttt ctcaaaaatt
     3541 gattcaaatt ttcggcgaat ttccgatagc ctttgccgtt ggcgtggatt tggtcggatt
     3601 tcagattatt attgcccaaa atttccgccc acgcgccgcc gaacaacgga atgccgtatt
     3661 cctcggacaa atcctcatac agcggatggt cgctcaaatg cccgaacaac gcgcccagtg
     3721 tgatgtgcgg cacgccgacg aggacggcgg gaatgttttc cttttgcacg gtttcgatga
     3781 ttttcgcgat attggcgcgg gtctgctcct cgggaacttt gcgcagaaag tcgttgccgc
     3841 ctatgccgac aatcacaagc ttgggtttgc gtgccaacag cgcgggcagg cgcgataggg
     3901 cttgcgcgga cgtatcgccc gatacgccgc cgttgacaat attccaaccc gtcagttttt
     3961 gcagttgcgc ggggtaggat tcgccggggt ttgctccgta gccgaaggtg agcgaatcgc
     4021 ccaaggcaag cacggtgctt ccttcgggaa ttttggcgtg ggttcgggcg gattttctgc
     4081 cgcaggcggt aagaagcaac gcgcctgcgc cgaggaggaa ggttcttctg ttcattggtt
     4141 tttcagacgg catcgagcca tttcgggtgg tatttcaggg ttttacggta aaccggacag
     4201 ctttcggcgt gcgcgccttt gaggtagccg gtactcatca gaaattcgcc gacgatttcg
     4261 ccgccgacga atttgaaatg ttttttaaag agtttgaccc attcgtcttt gcttcgcgga
     4321 tggtgcgcgt cgagccagtt cttgaacgaa ccgtgttctt gttgcaacgc ttggatttgc
     4381 cgtgcattga aaatggcggc atcgattttc agacggttgc ggacaatacc cgcatcggta
     4441 agcagccgtt caatatcggc ttcgccaaat gcggcgaccg tatcgatgtc gaaaccttca
     4501 aatgccgtct gaaacgcctg ccgcttcttc agcatcagcg tccaacttaa tcctgcctga
     4561 ttgatttcca acaccagccg ttcaaacagt ccgttgtcgt ccttaatcgg aaaaccgtat
     4621 tgcgtatcgt ggtaatgttt gttcgggttg tcggtgtttt cgggaagtga ggcggcaaat
     4681 tcgcaatagt tcacggttta ctcgaccatc tgttgcgccg cctgtttcag tccctgttcg
     4741 agtgcggcgg tcatggcggc gtagccgtca ccctgctgtt cggtttcgat atggaagggt
     4801 ctgttcgtac cgtcgggtag gacggcgtag ccgctgatga gggttttgcc cgtgtagctg
     4861 ccttggaatg cgtcgatata gaccgtccat ttgtcggtac tgccgctgcg tgaggcagga
     4921 acaaaggtgc gtgtgctgtc caaacggttg aatgcattgc tcaacgccgc ttcgagcata
     4981 tcgtccaagg tgtctgccca aacatggttt tgtgcggtgt tgatgcggta ggggtcggtt
     5041 tgatagacca gtccgccgcg tttgagcggt tcggcaagac ggacttcgac ggcggtttcg
     5101 ccgccttgcg ttgcaggacg gatgtagcgg ctgtcgggca acacgaaata ttgtgtgctt
     5161 tgcacagtac cgcaggcggc aagcgtcagg gcggcggcga tggggaaaag gcgcattatc
     5221 ggcttccttt cgggataggg tctttgctgc tgttgttgaa aatcagtgcg ttgggttttt
     5281 ctttcaaagt gttaatgacg ggttgaacgt cttttaaggt tttgtccaaa ctttgcagcg
     5341 tattttgtac gtctccgtag ataggcgatt gaggcgatac gccttgcagg gttatgcgca
     5401 actctttcag agtttggttc agttcgttcg ggatgttttg cgtctgcgga ttgccgacca
     5461 gtttgtcaat ggagcttagg gcggcattgg cggatttgag tgcggacttg agttcggcga
     5521 gcgagccgtt caattcggca acggttttat ccaatggcag attgttgaat ttgtccagca
     5581 aatccgccaa tttggcctgc aagtcgtcca aaccgccgcc tcgtgtggcg atgacggtat
     5641 cgcctgcata aacggtatgc ggtcgcagct tgggcgaggc ggaaggctga tcgttcaact
     5701 caatcatttt gccgccggtc agcaggttgt tgctggagat ggtggcggtc aggcctttgt
     5761 ttaaggccgt ctggaattgt tgtttccaat gctctttgct ttgctcgtcg gcattgattt
     5821 ccaaacggga aggctcgatg cggatgcgta cgggaatcca gccgttttca aacaggtgca
     5881 ggctgtcatt gcggtcaaaa taagggacat cggaaaccat gccgacattc agccctttgt
     5941 attcgacagg cgaaccgacg gtcagtccgc gcacggattg tttgaaaaac gcggtgtagt
     6001 acagcgagcg gtcgtcaggc aggttggcga tttcgctgcg gctgtcgtaa agcgtgaagc
     6061 tgtcctcgct tttgacgttt ttactgtttt tggttttcgg cgaatcaaat gaaatcgcgc
     6121 ctgacagcag ggcaggcaga ggggcggaat tgagtttgat gccgctgcct gtggtttcga
     6181 tattgatgcc gctttccagc caaaaacggc tggcggaatg aatcagtttg tcgttggggc
     6241 tttggatgaa gatggtgtaa tgcacgcttt ggtcggacgg gtcgaaatgc gcgctttcga
     6301 tttgcccgac cataaagttt tcatacaaaa cagggctgtt gacgttgagg atgcggtcgt
     6361 ttttaccaat caaattcaag cgcagcccgc tttgcccgat ggcggtaacg ggcggaatgt
     6421 cctgcacttg gaacacgtct tttgcctcgc cgcttttgcc gggtgtaaaa gcgatgtacg
     6481 aacccgaaag cagcgtaccc aaaccggtta cgccgctttg gtcgatacgc ggcttgacca
     6541 cccagaactg ggtatcgctg cggatgaggc cggatacgtc cgcattgagt tgggcagtaa
     6601 cttccacgcc tttttggtcg tcgcgcagtt tgattcgggt aacgcgtccg acatcgatgc
     6661 tcaatacctt aatgaccgta ttgttgactt cgatgccttc cgcgctgtcc atcaagagcg
     6721 taaccacagg ccccctgttg cggatttcct taacccaaag ccagccgccg gcaatcagcg
     6781 cgatcagcgg gaccagccag acggcggaga ggaaggtgtt gtttttgcgg acgcgtgctt
     6841 gagcgtgtcc gtttggagga gggctgttgt cagtcatatt tttccgtttc gttgaaagca
     6901 atgccgtctg aagcgcgttt gtcccaaagc aggcgcgggt cgaaataata ggcggacagc
     6961 atcgtcaaaa tcacgaccag gcagaaatag actgccgcac tgcccggaat gacgcgcgcg
     7021 gcataagtgt ggaacgaaca catcaaaata ataatcacaa aaatatcaat catcgaccag
     7081 cggccgaccg cttcggtgat gcggtagagg tgcgacaatt tctttgcgcc cgccggcaaa
     7141 gcgaaccgtg ccgccgcaat caaaaccgac attgccgcaa tcttcagcac cggcaccaaa
     7201 atactcgcgc tgaaaataac cgccgcaatc agcctgtcgc cctcgtccca catataagcg
     7261 atgccgctaa agatggtgtt ggcctccgtg gcggcaggat tggacgaaat cataatcggc
     7321 aggatattgg cagggaaata caaaacaacc gccgccgtca gaaacgccga cgaaatactc
     7381 agacttttcg gccgtcggcg gtacagttcc gcgccgcaca ccccgcaggg ggattcggca
     7441 ctgtcgcgga aatacaggca gcggctgcaa caggttttgc cttccgatgc cgtctgaacc
     7501 gcattattcc ccgtcagccg cccgatttgg aaatacaccc aatgctgggg aaccgatacc
     7561 gaagtccgaa tcagcataac cgacagcgcg aacatcagat aaaacgccgg cccgaagcga
     7621 acctttgcca cagacgagag cttgatatac gccaccagag tggaaacaaa aaacacatcc
     7681 accatcatcg cctgcctcaa gcgcaccatc acacgcgttg ccaaacgcag cgcaggatac
     7741 gcctgtttcc gtatcagcgc ggcatagaca tacaggcaca gcagcagaaa cagaaccggc
     7801 gcgccgaaag tcagcacaaa catcacttcg gccaaaaaac cataatcctg aaacaccatc
     7861 aggcgcatca tctcgggcag cgaaaggacg gatgccgcac ccggtatccc gacctcgata
     7921 tacgtcatac tgtaagcaaa cgccatcaaa atcagcgaag ccgccgcata ggcgggcgga
     7981 ccggaaaaag ggtggctgcc caccctgaag agtttgtgtc cgcaacgggg acagaacgcc
     8041 gcctctccct tgtccaactg gggcacgtct gtacggcagc cgcattccgg gcaatctacc
     8101 gtatgcggcg gcagggcttc gtcgcggcag ttgccgcgaa ggctgtgggg gatattttcg
     8161 gcaaacggct tcatcgggaa agtggcaaat ataaatggcg gcattataaa ggaaaaaaaa
     8221 gcgatacacc caatcaggat gcaaaaccgg caggttcggc agtatgaaca aacccttggt
     8281 gtccggcaaa aatatttttt gagcggctgc ccctccggcc gacaagccta tttgataaaa
     8341 ccccgatttt gtaaataatc ataaaatacc cgcttaaaaa tataacgcaa atgatatttt
     8401 ttcgtaaaat atgtgataat aaatttcgtt tatgattctt tattattttt gtatgcgggc
     8461 aagtccggat tttgcccgca ccgcatcctt atcccttacc gacaaaggag aacttatgag
     8521 cgaaaccgaa aaccaagccc tgacgtttgc caaacgcctg aaggcggata ccacggcggt
     8581 tcacgacagc gtggataacc tcgttatgtc tgtccaaccg tttgtcagca aagaaaacta
     8641 catcaaattt ttgaaactgc aatccgtgtt ccacaaagcg gttgatcata tctataaaga
     8701 ccccgagttg aacaaagcca tttccgagct ggaatacatg gcgcgatacg atgccgtaac
     8761 gcaagacttg aaagacttgg gcgaagagcc ttacaaattc gacaaagaat tgccacacga
     8821 aaccggcaac aaagccgtcg gctggcttta ttgcgccgaa ggatccaatt tgggcgcggc
     8881 atttttgttc aaacacgcgc aaaagctgga ttacaccggc gaacacggcg cgcgccacct
     8941 cgctccgcat ccggacgggc gcggcaaaca ctggcgcgcc ttcgtcgagc acctgaatgc
     9001 cctgaactta actccggaag cagaagcaga agccattcaa ggcgcgcagg aagcgtttgc
     9061 tttctataaa gtgattctgc gtgaaacctt cggtttgccg gaaggaacag aagcccccga
     9121 aggcatgatg ccgcacagac attaaaaatg gggctgtact agattatccc taaattccac
     9181 accgatcccg caggattttt agctgccggg acggtgtgcc ggagttaagt cgaaattcgc
     9241 attccctcaa caacggcggg aaaggtttac gatcgattcc gttgtatttt cgcaaggcgc
     9301 gttttgcctg attccaaaag ttcccaatgc cgttaatgtg gttccgacgg tctgcaaatt
     9361 ccttggaacg g
//
LOCUS       DAXFWM010000078         9115 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000078 DAXFWM010000000
VERSION     DAXFWM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.078"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            188..2464
                     /gene="znuD"
                     /locus_tag="ACFI8B_001897"
                     /gene_synonym="tdfJ"
     CDS             188..2464
                     /gene="znuD"
                     /locus_tag="ACFI8B_001897"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC3835043.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDNQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(3004..3783)
                     /locus_tag="ACFI8B_001898"
     CDS             complement(3004..3783)
                     /locus_tag="ACFI8B_001898"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC3835044.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            3937..6786
                     /gene="uvrA"
                     /locus_tag="ACFI8B_001899"
     CDS             3937..6786
                     /gene="uvrA"
                     /locus_tag="ACFI8B_001899"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC3835045.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEEVAKVKGSYTGKYLKVVLK"
     gene            6804..7445
                     /locus_tag="ACFI8B_001900"
     CDS             6804..7445
                     /locus_tag="ACFI8B_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFC3835046.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            7449..8582
                     /locus_tag="ACFI8B_001901"
     CDS             7449..8582
                     /locus_tag="ACFI8B_001901"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3835047.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
     gene            8890..9078
                     /locus_tag="ACFI8B_001902"
     CDS             8890..9078
                     /locus_tag="ACFI8B_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835048.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHTKNLGAVLDYP"
BASE COUNT         2477 a         2565 c         2110 g         1963 t
ORIGIN      
        1 cggcgttcgt gttcacatca ttaacttccc taaaattagg gttaatacaa attaacgcca
       61 ttaaatatca caaccgtgtc atttgaaaaa cttttgccta tgccctctga gtattctcaa
      121 aatcttatga tataaaaaat gcaatgttat ataataacaa acttttgaaa tgcgacgaga
      181 agccaaaatg gcacaaatca cactcaaacc cattgtttta tcaattcttt taatcaacac
      241 acccctcctc gcccaagcgc atgaaactga gcaatcggtg ggcttggaaa cggtcagcgt
      301 cgtcggcaaa agccgtccgc gcgcgacttc ggggctgctg cacacttcga ccgcctccga
      361 caaaatcatc tccggcgata ctttgcgcca aaaagccgtc aacttgggcg acgctttgga
      421 cggcgtaccg ggcattcatg cctcgcaata cggcggcggc gcatccgctc ccgttattcg
      481 cggtcaaacg ggcagacgga ttaaagtatt gaaccatcac ggcgaaacgg gcgatatggc
      541 ggacttttct cccgatcacg ccattatggt agataccgcc ttgtcgcaac aggttgaaat
      601 cctgcgcggg ccggttacgc tcttgtacag ctcgggcaat gtggcggggc tggtcgatgt
      661 tgccgatgga aaaatccccg aaaaaatgcc tgaaaacggc gtatcgggcg aagccggatt
      721 gcgtttgagc agcggcaatt tagaaaaact gacatccgca ggcatcaata tcggactggg
      781 caaaaacttc gtgctgcata ccgaaggctt gtaccgcaaa tcgggcgatt acgccgtacc
      841 gcgttaccgc aatctgaaac gcctgcccga cagccatgcc gattcgcaaa cgggcagcat
      901 cgggctgtct tgggtgggcg aaaaaggctt tatcggcgca gcatacagcg accgtcgcga
      961 ccgctacggc ctgcctgccc acagccacga atacgatgat tgccacgccg acatcatctg
     1021 gcaaaagagt ttgatcaaca aacgctattt gcagctttat ccgcacttgt tgaccgaaga
     1081 agacatcgat tacgacaatc cgggcttgag ctgcggcttc cacgacggcg acggtgcaca
     1141 cgcacacacc cacaacggca aaccgtggat agacctgcgc aacaaacgct acgaactccg
     1201 cgccgaatgg aagcagccat tccccggttt tgaagccctg cgcgtacatc tgaaccgcaa
     1261 tgactaccac cacgacgaaa aagcaggcga tgcagtagaa aacttcttca acaacaaaac
     1321 acacaacgcc cgtatcgagt tgcgccacca acccataggc cgtctgaaag gcagctgggg
     1381 cgtgcaatat ttgggacaaa aatccagcgc gctttccgcc attcccgaaa ccgtccaaca
     1441 accgatgttg attgacaaca atgtccgcca ttacagcttt ttcggtgtag aacaggcaaa
     1501 ttgggacaac ttcacgcttg aaggcggcgt acgcgtggaa aaacaaaaag cctccatccg
     1561 gtacgacaaa gcattgattg atcgagaaaa ctactacaac cagcccctgc ccgacctcgg
     1621 cgcgcaccgc caaaccgccc gctcgttcgc actttcgggc aactggtatt tcacgccaca
     1681 ccacaaactc agcctgaccg cctcccatca ggaacgcctg ccgtcaacgc aagaactgta
     1741 cgcacacggc aagcacgtcg ccaccaacac ctttgaagtc ggcaacaaac acctcaacaa
     1801 agagcgttcc aacaatatcg aactcgcgct gggctacgaa ggcgaccgct ggcaatacaa
     1861 tctggcagcc taccgcaacc gattcggcaa ctacatttac gcccaaacct taaacgacgg
     1921 acgcggcccc aaatccatcg aagacgacag cgaaatgaag ctcgtgcgct acaaccaatc
     1981 cggtgccgac ttctacggcg cggaaggcga aatctacttc aaaccgacac cgcgctaccg
     2041 catcggcgtt tccggcgact atgtacgagg ccgtctgaaa aacctgccgt ccctacccgg
     2101 cagggaagat ccctacggca aacgtccctt catcgcgcag gacaaccaaa acgccccccg
     2161 cattccggct gcgcgcctcg gcttccacct gaaaacctcg ctaaccgacc gtatcgatgc
     2221 caatttggac tactaccgcg tgttcgccca aaacaaactc gcccgctacg aaacgcgtac
     2281 gcccggacac catatgctca acctcggtgc aaactaccgc cgcaatacgc gctatggcga
     2341 gtggaattgg tacgtcaaag ccgacaacct gctcaaccaa tccgtttacg cccacagcag
     2401 cttcctctct gatacgccgc aaatgggccg cagctttacc ggcggcgtaa acgtgaagtt
     2461 ttaaaatcgg acaggcaaac aaaaaaggtt ttcagacggc attctgaaaa cctttttttc
     2521 tttttaatca acttgttagg aaatatggca aagacaagga acaaaccact gtgccatcat
     2581 tcccattttc tttgtttcat cattcttgct ttcacgccgt cattctcgca aaagcagaaa
     2641 aatcaaaagc aaaaacccaa gatcccatca ttcccgcgca ggcgggaatc caattcattc
     2701 ggtttcagcc gtttccgata aatcaccgta acgttaagtt cccggattcc cactttcgtg
     2761 ggaatgacgg cgtaaaggtt tctgtttttt aagttttagg taactaatga gtcgtcattc
     2821 ccgcgcaggc gggaatccaa ttcgttcggt ttctgttatt tctgataaat caccgtaacg
     2881 ttaagttccc ggattcccat tttcgtgaac ataacggtgt gaacattagc aaaaattcaa
     2941 aaaaccaaac ttcctcattc gtgtgaatga tggtactgat attttctatt tcagttcgca
     3001 aaattaatct ttcaacacat cttgcacagc cgcctgaatc ttagccgccg ccgcttcgat
     3061 ttgctgctgt tcggcagatg ggtttgccgc tgtttcaacc gaagcagctt gtgaaacagg
     3121 atcggcttga gaatcagtca gcggcaaacg cgccaatacg gtttttacgc cggttacttt
     3181 atcaccaatc gccacttgcg cctgcgcatc gacaggcaga tacacatcca cgcgcgaacc
     3241 gaagcggata aagccatagc gttcgccgcg ggacagtttc gcacctgctt gggtgtaaca
     3301 caaaatacgg cgtgccacca gaccggccac ttgaacaaaa gtaatttcac gaccggaagc
     3361 cgtagtcgcc aaaaccgcgt tgcgttcgtt ttccgtgctg gctttgtcca aatccgcatt
     3421 cacgaatttg cctttgttat agaccacttt cgttaccgta caatcggcag gcgatttttg
     3481 cgaatgcacg ttgaacacgt tcataaaaat actgattttc aaagcatcga catcacgata
     3541 cggatcgcgt gcgcgctcga ccaccacgat acggccgtca accggactca acaccgcttc
     3601 aggattttgc ggaatttcac gcgcagggtc gcggaaaaac tgcaatgcaa atacggtaaa
     3661 cacccaaaac ggcaaagacc accagccgca gcacatcgac accagcaggc tcaaagccaa
     3721 accgccgccg ataatcggcc agccctcacg ggcgataatc gggtggggat aaagacggtt
     3781 catttgtcat ccttttattc aaatatatta aatagcgaca ttataacgga atccgccgcg
     3841 ttccgccgac aaatgccgtc tgaacgcggc ttcagacggc atccgagaaa gtgctacaat
     3901 atcggttttc ctttattttt tcaggcggca tccattatgt gcaaccatca tccccgacat
     3961 tcgcacgaca acgacaccat ccgcattcgc ggcgcgcgca cgcataattt gaaaaacatc
     4021 gatttggaca ttccccgcca caagctcgtg gtggtaacag gattgtcagg cagcggcaaa
     4081 tcatcgctgg cgtttgatac tttatatgcc gaagggcagc ggcgttatgt cgagagcctg
     4141 tctgcctatg cgcggcagtt tttgcagatg atggacaaac ccgatgtcga tttgattgaa
     4201 ggcctgtcgc ctgcgatttc catcgagcag aaatccacca gccacaatcc gcgttccacc
     4261 gtcggcacgg taacggaaat ccacgactac ctgcgccttt tatacgcccg tgtcggcacg
     4321 ccgtattgcc ccgaacacaa gctgccgcta tccagccaaa ccgtatctca aatggtcgat
     4381 gccgtattaa aactgccgga agatacgcgc gtgatgattc tggggccggc ggtgcgcgag
     4441 cgtaagggcg agtttgtcga tttctttgcc gacttgcagg cgcaaggctt tgcccgtgtg
     4501 cgcgtggacg gcgaagtcta tcagcttgac gaagtaccga agctggaaaa aaacatcaaa
     4561 cacaatatcg acgtcgtcat cgaccgcgtg aaagtgaaag ccgacatcaa acaacgactg
     4621 gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc gcgcgctggc gatggaaatg
     4681 gacagcggcg aagaacattg gttttccgcg cgttttgcct gccccgtatg ctcatacagc
     4741 ctgcccgaat tggaaccgcg cctcttttcg ttcaacaacc cgatgggttc ctgcccgact
     4801 tgcgacggct tgggcaacac caatttcttc gaccccgaaa aagtagtcgc ccatcccgaa
     4861 ttgtcgctgg caaccggcgc gattgacggt tgggacaaac gcaaccaatt ctatttccaa
     4921 atgattcaat cgctggcgca tcattataaa tttgatgtca acgtcgcttg ggaaactttg
     4981 cctgaaaaag tcaaaaaagt cgtgttgcac ggctcgggca aagaagtcat tgatttcacc
     5041 tacctgtccg aacgcggcac caccttcaac cgcagccacg ccttcgaggg catcatcccc
     5101 aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg tgcgcgaaaa actgcgcgag
     5161 taccaaaatc accgtgcctg cccgagctgc ggcggcgcac gtttgcgcaa agaagcacgc
     5221 tacgtttacg tcggcggcga accgttgcac gaagtctccg cctggccgct gaccaaaacc
     5281 caccgattct tcgaaacgct ggatttggac ggcaacaaaa aacagatcgc cgaaaaaatc
     5341 ctcaaagaaa tcaccgagcg gctcggcttc ttgattaacg tcgggctgga ttacctgaat
     5401 ttaagccgtt ccgccgaaac cctttccggc ggcgaagccc agcgcatccg cctcgccagc
     5461 caaatcggca gcggcctgac cggcgtgatg tacgttttgg acgaaccctc catcggcctg
     5521 caccagcgcg acaacgaccg cctgctcgcc accctcaaac gcctgcgcga tttgggcaac
     5581 agcgtaattg tggtcgaaca cgacgaagac gccatccgcg aagccgattt cgtcgtcgat
     5641 atgggccccg gcgcgggcga acacggcggc aacgtactga ttgccgacac acccgaaaat
     5701 gtcgccaaat gcgaaaaatc cgttaccgga caatacctcg gcggcaaaaa atccattgcc
     5761 gtgccgtctg aacgcacgcc cgtcaatccc gaccgaatgc tcgtcctcaa aggtgcgcgc
     5821 ggcaacaacc tcaaaaacgt caccctcgag ttgccgctcg gtttgattac ctgcatcacc
     5881 ggcgtatccg gcagcggcaa atccaccctg attaacgaca ccctcgccaa aatcaccgcc
     5941 cgcgaactca accgcgccca agaagaaccc gccccatacg acgacatccg cggcctcgaa
     6001 cacctcgaca aagtcatcaa cgtcgaccaa tcccccatcg gccgcacccc gcgctccaac
     6061 cccgccacct acaccggcct gtttaccccc atccgcgaac tcttcgccgg cgtacccctc
     6121 tcgcgcgaac gcggctacaa cgtcggcaga ttctccttca acgtcaaagg cggccgctgc
     6181 gaagcctgcc aaggcgacgg cgtaatcaaa gtcgaaatgc acttcctgcc cgacgtgtac
     6241 gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg aaaccctcga aatccaatac
     6301 aaaggcaaaa acatcagcca agtcctcgac atgacggtcg aagaagcccg cgaatttttc
     6361 gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga tggacgtagg tctcggctac
     6421 atccgcctcg gccaatccgc caccaccctc tccggcggcg aagcccagcg cgtcaaactc
     6481 gccttggaac tctccaaacg cgacaccggc agaacgctct acatcctcga cgaacccacc
     6541 accggcctgc acttcgccga catcgccctg ctgctggaag tcataggccg tctgaaaggc
     6601 aaaggcaact cgattgtgat tatcgagcat aatctggatg taattaaaac cgcggattgg
     6661 attgtggact tagggccgga aggcggcgat ggtgggggta aggttatcgc gaaaggtagc
     6721 cctgaggaag tggcgaaggt taaggggagt tataccggga agtatttgaa agtggttttg
     6781 aagtaaacga acggatatag aaaatgaaca ataattttga ccgcagcaag catatctatc
     6841 ataacgaagc ttttgtagaa ctggttaaag atgccatacg cttttttcat ggtacgcctg
     6901 ttcatacact tcctccaccc aaacagttca aaggtgcagg ggtttatgcg atttactata
     6961 tcggtaacaa tcctttgtac aaacaatatg cggactggaa cagattatcc tacaatgcgc
     7021 caatttatgt tggcaaggcc gtccccaaag gctggcggca agcaagaaat tctgataatg
     7081 cgctaaacca atcaaccgaa ctgttccacc ggttgaaaga acacagcagg agtattgccg
     7141 ctgtttctga tttagatcct tccgatttca tgtgcagatt cgttatcttt gaaggtgccg
     7201 gttctgacat gataggcaca atcgaagctg ccctgattaa attacacaaa cctctatgga
     7261 actcttgtgt cgatggtttc ggaaaccacg accccggaaa aggccgttac gaacaagcca
     7321 aatcggattg ggatgtcctt catagcgggc gggtttgggc agataggcta aacggcatac
     7381 cgaatagcta tgagtccata cttgaaaata tcaacacaca tttagagata ataaagagaa
     7441 aataacctat gaagtctttg gaaatttttt ccggcgcggg cgggttggca aaaggccttg
     7501 aacttgcagg attccaacat gcatctttta ttgaactcaa taaagatgca tgcaattctt
     7561 taagaagcaa ctttaacccc aagcttgttt atcagggaga tgtggcagat tttgatcttt
     7621 ccagtcaaga aggcatagaa gtcattgcag gcggtcctcc ttgccagccc ttttcattgg
     7681 gcgggaaaca ccttgcccat gaagacagaa gggatatgtt tcctcatgcg gtcagatatg
     7741 tggagtatta ccgccccaaa gcttttatct ttgagaatgt aaaaggactt ttaagaaaat
     7801 cattcgctga ttattttgaa tatatcctac ttcggcttac ttatccgaat cttggtattt
     7861 tgcagaatga agattggaaa ggccatctga cacggctgaa agaaattgaa ttcaatttat
     7921 ataaaggaat caaatataag gtttcatatc aattgttgaa tgcagctgat tacggagtgc
     7981 cgcaaaaacg cgaacgcgtg gttatcgtag gtatccgggc cgatttggat attgattgga
     8041 aattcccaaa aagaacacat tccgaagaca ggttgaattg ggaaaagtat gtaacagggg
     8101 aatactggga aaaacacaac gaacccaaaa gattcaataa agatattgct gaaaagttac
     8161 aaaaaaaata cggtatattc gaaccagaaa aaaaaccttg gcaaacggta agggatacct
     8221 tgtccgacat cccgcatcct ttggggaatc ataaaattac aggacatgaa tatagggatg
     8281 gcgcaagaat ttatcccgga cacacaggaa gcgggataga cgaaccgtcc aaaaccatta
     8341 aagcaggtgg gcatggcgtt cccggcggag aaaatatgat tcgttatgat gatggaacag
     8401 tcagatactt taccagctat gaagcaaaac ttcttcaaac attccctgaa gaatttgtca
     8461 tttctggagc ttggggagaa gcaatgcgac aaattggcaa tgcggttcct gtcaaattgt
     8521 cggaaatttt aggcaaacat ctgatggggg tgttgtcgga gaaaagcagc ctgcacaact
     8581 aacccgccca ctgcgtgtgt gcggtacgca cgcggttgct tgattgacgg tttgccgttt
     8641 caacttttaa cttcgttgaa gctagtgctt tcagacggcc tccaatgtcg tttggaaaca
     8701 tcaggtccaa caaagttaaa ccttaaacaa caaaccattc cttctccaac acccaaacca
     8761 caaacaaaaa tcctatggcg cgttatcgca gaaacttcat tgccggcggc acattctttt
     8821 tcaccgtcaa acccgccgcc ccgaaatcgc gtctgcttgt cggacatatc ggcttcgcgt
     8881 gcggcttata tggatgtgca aaaacaatat ccctttgaaa ccgtcgccgt atgcgtgctg
     8941 ccgaaccata ttcacgccat ttggacgctg ccgcccgacg atgcggatta ttccctactc
     9001 cggcggctga ttaaaaccaa attctccgca tattcccctc atactaaaaa cttaggggct
     9061 gtactagatt atccctaaat tccacaccga tcccgcagga tttttagctg ccggg
//
LOCUS       DAXFWM010000079         8569 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000079 DAXFWM010000000
VERSION     DAXFWM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.079"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..183
                     /locus_tag="ACFI8B_001903"
     CDS             <1..183
                     /locus_tag="ACFI8B_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835049.1"
                     /translation="YCHEFQKMNYGTSVGIGSDSFTFFKGISPVPKIDYCSKCGKPLS
                     PEAVNKVMNDKTDEEM"
     gene            complement(354..2096)
                     /locus_tag="ACFI8B_001904"
                     /pseudo
     CDS             complement(354..2096)
                     /locus_tag="ACFI8B_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            complement(2314..8387)
                     /locus_tag="ACFI8B_001905"
                     /pseudo
     CDS             complement(2314..8387)
                     /locus_tag="ACFI8B_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
BASE COUNT         1890 a         1996 c         2012 g         2671 t
ORIGIN      
        1 tattgccacg aattccaaaa gatgaattat ggcacctccg tcggcatcgg gtcggatagc
       61 tttactttct ttaaaggcat ttcccctgtt ccaaaaatcg attactgcag caaatgcggt
      121 aagcccctgt ctccagaagc cgtaaataag gtgatgaatg ataaaaccga tgaagaaatg
      181 tgaacagcgt tttaacaaca gtgaaataaa atataaaatt tcaattttaa aataatgagc
      241 taatggaaat ttgacccggt tgtccggtac ggccccaaac ataaaacaaa agctgcctgc
      301 aaaaaaccgg cagcttttgt gtatcttgtc atacattatt cacttcaacc gcatcaaaac
      361 gaataaccca cctgaaaccc cgttacccat ttcttcgtct gaaaatattc gggctttttc
      421 aatgcacggc cggtaaatat atcgtaatgc aggttgccgc caagctttat ctgcccgcgt
      481 atcccaattg ctgtgccggc tagagtttgg cccgataacc atttggcgga ttgtcctgaa
      541 acatgtccta catcagcccc aagataaagc tgatggcctg gtttaaattg ccagctcaaa
      601 tcgttgcgcc aataccatcc ccgctcggca ggcaaactca tttcaccgtc gaagccacgt
      661 acggtgtggt gtccgccgat agccagtttg tcttgcgatg ttagcggggt tttgttccat
      721 tgtgcatgaa cggatgtgtc ataggcaaat agctgtttac cgatttgaaa aggagtattt
      781 acatcagccg atgccgtcca aattttcata cgtgacgtgc cttcgccaaa ggcttcttca
      841 ggcgcgcgca gagcatcttt catgccggtg ccgtgtttat atttcaactt aaaatctgcc
      901 gtactgcgac cgatatatcc tttgtgggaa agttctgcca accaacctgt ggttttacgc
      961 cgttgtacag tcagttcggc atcatcaatg taactttttg tttccctcgt ccacagtttt
     1021 acactgagat aggttttgcg tttggcatca cgatacaaca ggcggttgaa gccgaaatca
     1081 gtgttgtaac tttttccatt atagtcatag acttccgata atccggaaac cgcctgatgg
     1141 taacggtagc cattgtgatt gaatgcccat gtccatttac cgaaaggggc tgaataatgt
     1201 acggcgtaat tgtttgatcc gccttctttg cgatggccgt caaaattttc ctcatcgggc
     1261 gtaccgccaa ttgaacgtcc ataatttaca tagaacatat cactcagtcc aaaaggattg
     1321 tcggcagaga aagtgatatt tccttggtat tttcctgtcg cctcactacc cgaattatcc
     1381 atccccacac tcacacagta gggcagcaga cgttaccgcc attgcaccac gacatcactt
     1441 tggtttggtt ctctctctac gggaacgatt tggagatcgg cttccgcagt cgggagacat
     1501 ttgagatttt ccagtccttg ttccaaatca cgcagattca acagatcgtt cgagcgggtg
     1561 ggaaatttgt tttggaatgc tgcaatacgt cctgcatggg tttgatcatc gttggaccga
     1621 tcgattcgta tggagcgcag atagcccggc atcagggtta attgaagctt gccactattc
     1681 aaatcctgtg gcgcagccaa gatacgggtc gtggtatatc ccctgccgat caaagcattt
     1741 tgtgctaagg acatgatttg attaatgtcg cccgcatgca gacacttgcc ggaaacaaaa
     1801 tgtgtttggc acaaggcatg gttgagcgca aaccggaatt tagccgcttc ttcacccacc
     1861 aattccacct cagaaatagg aaagcagggg cttgcctcat ccgaatttgc accgcctacc
     1921 tgtgttgcca tcttctctat gcccgtatcg gtaccatcca aacgaacatc ttgttccggc
     1981 tgcatctgtt cacgcaattg cttctcacgt tgacgttgga gaatatcctg ttgttgtgaa
     2041 acagcttcag gtgtgggggc ggctgatgtt agggtgggga ggcagccatc aatgatagga
     2101 ctaacagtga taagggatag ggatgaaata catttttgga ggaaaagaaa ggtttcatag
     2161 gattggtcta agtaatgtaa gcgggcaatt atagcaatgc acaaaaacaa acaaaaagat
     2221 aaaaaaactc cataaatgaa aatttaatca catgtttata ggatatgaaa aagaggaagc
     2281 gaaatctgaa gccgtctgaa aacaacccca cacagcatcg gcggacgagt ttccctcagc
     2341 tgctccggca gccgtaccca gcatccttgt aatcgccgaa acggtctctt tttcctccgc
     2401 attcaggctg ccgccgtctc ctttgccgta cagccatttg ccgatgattg gggcggccgc
     2461 ttccgacccg cccgcaccca atgctcctgc cggggcattg ttgcctcatg ctgcggcaac
     2521 cgctgctcct aataccgcgt gggcaagaac gtgagcggtt tcttgactgg cggttagttt
     2581 gccattcgcg ttttgaccgg ctaaatcttt aaagtgctgt ccaatcgcat acgatacgtc
     2641 tggcgatgcg gtagccgcag cgatgcccgc tccgctttgg gtcggctcag ctaaacctga
     2701 ggctaacatg ttgagaatga ctttgccttg ttgccaatta tctgcttttg ctgccgcatc
     2761 ttgagcttca tgggctttgc gtttggcagt ttccatatcg ccattggcta atgcctcggc
     2821 tgctgctgtt tcggctgctt ctttgtctgc tttgagtttg tctaaatgtt ggttaatctc
     2881 ggtattggtt tgttgaacat ttttactaaa atcttggctg acggttcttt gtaaatccag
     2941 ttcactttgc actctatctt tgtcaaatat gtttttcagg ctgcccgaat gtcgttcggc
     3001 ggtgtctgtg gttacgtttg tatcaatatc ggctttggtt tgtgccgcta ttttgcctgt
     3061 cagcctgatt tgtgcggctt cgtctgtgat ttgaatgttt ttgggtattg atgccgcttt
     3121 ttgtgatgct gctttgactg tcgctgtcgc tgccgtaccc tactgatgaa cttgcgccgt
     3181 ttttatcggc tatgcttgtc aggtgtttgt cttgaggttt attttttgcg ccctgtccca
     3241 gtgttttgcc gcttacggca acgctggcac ccaatccaaa actttttcct tcgtattggc
     3301 tgtaattttg aatatcactg ccggcgagtg tgcctgtgct gaatcggttt ttacccttgt
     3361 cttttgcgct ttggctgctg gtgatgatgc cgcctttgag gcctgtatgg tttccgacct
     3421 tgatttgata gccgtcttct ccggcataaa taccgctttg ctcggttacc gaagcatggt
     3481 cggctcggat tttgctttgg ctgtaatcgc cactggcact gaagccataa cctacggtaa
     3541 cttgtgcacc ggcgttttgt tgtttgcttt gataagtttc tctatcttgt acactttgaa
     3601 tgcttaggtt tttggcattg acttgtacgc ctttgccgcg tacttgcgcg cctttgatga
     3661 tggtatcgcc accactttgg ataagggttt ggctgccttt gtcgccaata tggctatggc
     3721 ggtgggttac gctatcgcca tagccgtagc ctttgccgac attgccgcct gcggcaacgc
     3781 ctaatgacca gcctccttgt ccgaatgata cggcagcacc tgcgttccag cctgctgatt
     3841 tgttttggct gcgttcggta ttgctttgct cggctgattg cagatggatt tgcttatcgg
     3901 caatcaggcc ggttcctgct ctgcctgaaa cacctgagcc tgtgatgttg atattggatt
     3961 gttcgccgca ccttcggcaa taagggtagt tttgccgcct gcttgaattt gactcgcttg
     4021 ggcttgattg gcttgaactt gggtggtttg tcggttttgc tgttcgccgt aggttatgga
     4081 gatgctgact tgtttggcat tggttgtacc attggctaag ttttgtgcgc ctttgcctgt
     4141 ttgataggcc tgccaacctg cattggcagc cgccatggca ttaacgcggt cgtttttgct
     4201 ttgtccgact tgtttgctgc tttgtgtgct acggcaatcg cttgttgtgc caaatcggta
     4261 acgggcgaac tgaatgccac cgttaagcct ttttgttcgt aggtttgggt ggttttgctg
     4321 tttaattggt tttgtgctgc gccaatatcc atactttgcg tgctgataag gttgttgccc
     4381 tcagggctgg aaacgttgct gccggtttgt tcgtagtgtt tgcttgcaac aatggtggta
     4441 tcgcctttca ggctgcctac ggtactgcct gtatgttcgt tgctttggga ttggttttct
     4501 tgtgtgtttg tcttgctgcc aatagtgaag ccgatacctg cactcatcaa tcctgatttt
     4561 tgggtttgat gataggtttc gctttggctt tgagtttggg ttgtaccaat gcgaacatga
     4621 ttgcctgctt gaatccgggt gccattatcg gaaataacat tactgccaag gatgttggca
     4681 tcgtttcctg cctgcaatac aacttgcttg ccttcaaagg tgctgctttg agcagtttcg
     4741 tgatgacttt gggctttatc ggtaatgact aatttattac cgccgccgct tctgcctgta
     4801 tgtttggacg catcatcaac ttggccggca tggatgcctg agctgatagt aatgtcattt
     4861 ttagcataca cggcaagtgt gccttttgcg ctgccgactt cggcagcttt ggcattgaga
     4921 ttattccctg acaatagggt aacatcgcct tttgtttgaa tgctgctgcc gacttcgttc
     4981 gttgaacctc ggatggtatg gttatcggca tcaaaatgga tttcttgata tttgccggtt
     5041 tgtaccgtat ccaggttaat gtcgcgtcct gcctgcagcc gggtttgccc ttgatctgat
     5101 tgattgctga tttgaccggc aatgatgttg atgtctttgc ctgcctgcgc tgctaaaaca
     5161 cctttttctt tgcctgtgat ataaatacct gccattcggt ctaggtaggt gctgctacct
     5221 tgtgcatttt gactgctctt ggccgtgctt tggttgttga tgttgttacc cgcattgagc
     5281 aataatgtct gttcggcaga aagaatgccg ccaatattat tgatgtcttg tgtggccgta
     5341 accgctgatt tttgcgcatg aatacgccca ccgatattgt ctagcgtatc ggtattgata
     5401 ataagcgcat tgcgccctgc aatcgtgcct gagtttttca ggctgcctga aacattgatt
     5461 tgtgtattgc tgcctgacaa caatgcacct ttaccgtcta tgccgccatt tttaacgcgt
     5521 acataaacct gtggcatcaa tacggtttgt gtgccgccat caggaagttt aacttctttt
     5581 tgtaccaacc aaacaatatc gctggtcagt tgcgctgctt gctcggcact taatgcaatg
     5641 ccaacgctga gattcatcga acgtgccgca gtcgcgccat tatccattaa ggctttaaat
     5701 tgttcttcgt cgttttgata accgtctaaa cgacgatgcc ctgtcagctc tgcgatttgt
     5761 tcattgatta aacgttgctc gtaataacca tcacccaaac gtttatgtaa attgtttggg
     5821 tctagtttga ggctgcccag catatagtca ctacccaacc attgacggta gttggcaaag
     5881 cgtggatcgg tttcaacaag atagccttta ttggcaggat tgataatgta taagctgctg
     5941 ccgggtaatg gggtaaaaga attgggcgta tagggtagcg aaataccgtt gcttttcgca
     6001 gtacggatat tatcccggtt actttgtggc aactcagtgc cttggctggg cgcatgacgg
     6061 cttaatgctt tgctatgcga ttcataggca aatgaaccca gtgaaatgtc gcgtgtgatt
     6121 tcctccggca aagtataatt ttgttcacga tgccctgttt catcatgtcc tttacgacgc
     6181 gcacgccagt agttgtgcaa cttaccattt tcgctgaaga ctttcttctc gccaaaggtt
     6241 tgctcgttat gcaaaccgtc tttttctgtt tgcacaagca attgccgccg gcaatgattc
     6301 ggctgtcgct gttgaagact gctttgctat caataatcaa atcgctacct gcaatgattt
     6361 tagcaggcgc agttccggtt acttgagttt cttgcgttac tttttcataa tcgtatttat
     6421 gccaattttc atgcgccact ccatcagggg tgcgtaagtg gtctgattca ttgttgtaga
     6481 caaaccagcc taattcatgt tgcgtgcctt ctcgcaataa ttcgtgtcgt ccaaatgctt
     6541 cgtaatcaac aatacgctcg cgccctgttt ctaccaactg cgttttcaaa tgctcattgg
     6601 tattgtgcag cttttctaca cctaaacgca ttttgcctgc agcttcaatg attgcgccgg
     6661 cattgtgtat cctttgggct ttgcctgtgg cttggtcatt ggtatctaat gcgccgccaa
     6721 ccgccatatc gttaccgctg taaatcagac tgttttcacg gttatttaat tgttcaatgc
     6781 ctaaattcag gttttcgcgt gccgcaatgg cggcacctgt accgttttca tcttgattgt
     6841 ctaagcgggt agccgcaata gcgatattgt cgccataaat ccgacctgta ccgatattat
     6901 tcatttgccc ggcttggatt ttggtttgtt gtccgtcaat caagcctcta ttggttaaat
     6961 tgtgctgcgt gccaatgtct gtcgtaccgc cggattgaat gttgccttgt actgcattat
     7021 caaggttatt tgctttaatc cgaatgcgtt ttcctgcttg caaggtatgt gaatttttca
     7081 ggctgcctcg tgtactgagc gacaattcat tgcccgccac gatcttgcgt tctacataaa
     7141 aatcatcttg taacgcaata tccagtttat tatcagcggc aagtgtgccg ttgttggata
     7201 acgattttgc ctgaatggca acatcacggc ctgattgtat cgtgccattc gtattatcaa
     7261 tgacaacggt agattgctga ccatcgtgaa taatcagttg ttgattggtc gctatttcgc
     7321 cattttgatt gttcaggctg cctgaaacgg ctaaatcctc tgttcctgcc gacaataatt
     7381 tgccagtgtt ttgaatcgat tgactgtgaa tttgagtgcc ttgttgcgat accgccgtac
     7441 cgctgttttc aaaggcttga ctgcggatat tgactttgtg ttccgctgta ttattcatat
     7501 cttgcacatt ggcggcagcc atcgtgccac tattgaccaa acggccattt gcatcaatcg
     7561 ccacattacc ggaagaagca aacaactgcc cttgattacg aatgcctgct tgttcggccg
     7621 tactgatcaa ggtgattttg ttggcataca agaatacgtg tgaaatcggt atcacgggca
     7681 tccaaaccgt gtccggcgat tacaacattg ccttgcctta tcttaaagcc gctaaagtct
     7741 cctgcttgat attgcggttg gcctgtcgtc aaagtggcac gggaagcatt gataaaacca
     7801 ccaccattga ctgcaatccc tgccggattg gcaataacga cttctgcacg tcgtccaccc
     7861 acttcaatat agccattcag ttgtgaagga tggctgctgt tgatttggtt tacaaccaca
     7921 cgtgcttcgc cccttgtcaa ccaaggattg ccttgaatcc aaccgcctag ctgtgtttgg
     7981 gtgttgctgc gactgttgtt taaaatcgcc ccgcgattac ccacatcaaa ctgggcatat
     8041 tgattaacag aaacccctgc cgaagtaggg gtttgaatat tgacttgcgg tatgccgtta
     8101 cctgtttgca gaatcgtggc ttgttgggtt ttaggagcag ctttatcagt aataatgccg
     8161 tcagcaaaag caatattgac cgtacccaaa gccaaacata aagaaaagcc taatgcagaa
     8221 aaacaaaagg ctttggaatg agtaggaatg aaagaaacgg atttcacata aacgctgccc
     8281 gaaccactat cggcacagct tttaccttcg cgcttggtgg tttcggcaac agctaccaca
     8341 gcaccgcgtt tgcggttgaa aatcacacga tagagggttt tgttcatgat tttagttatt
     8401 tgatttttat agagttatta caaacattgg acagtctggg cattctagga caaagatttt
     8461 ggtaagtcaa ttaccgccat ttttttatta aattagggga cgtaccggat aacggctaaa
     8521 aatcccattt aggttagaat aagccaatga gaaaagccgt ctaagccgg
//
LOCUS       DAXFWM010000080         8445 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000080 DAXFWM010000000
VERSION     DAXFWM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.080"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..197
                     /locus_tag="ACFI8B_001906"
                     /pseudo
     CDS             1..197
                     /locus_tag="ACFI8B_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1275 family protein"
     gene            345..570
                     /locus_tag="ACFI8B_001907"
                     /pseudo
     CDS             345..570
                     /locus_tag="ACFI8B_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            680..1357
                     /locus_tag="ACFI8B_001908"
     CDS             680..1357
                     /locus_tag="ACFI8B_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC3835050.1"
                     /translation="MTFFKPSTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1631..2401)
                     /gene="xth"
                     /locus_tag="ACFI8B_001909"
     CDS             complement(1631..2401)
                     /gene="xth"
                     /locus_tag="ACFI8B_001909"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC3835051.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMSRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2461..2742)
                     /locus_tag="ACFI8B_001910"
     CDS             complement(2461..2742)
                     /locus_tag="ACFI8B_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC3835052.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLTAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2995..3246
                     /locus_tag="ACFI8B_001911"
     CDS             2995..3246
                     /locus_tag="ACFI8B_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835053.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDTLDRCSG"
     gene            complement(3309..3449)
                     /locus_tag="ACFI8B_001912"
     CDS             complement(3309..3449)
                     /locus_tag="ACFI8B_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835054.1"
                     /translation="MSGKEPLNRNPVIASGRECRLKYSSDGIFREGRDALEKTAFQAG
                     AI"
     gene            complement(3477..4358)
                     /gene="nadC"
                     /locus_tag="ACFI8B_001913"
     CDS             complement(3477..4358)
                     /gene="nadC"
                     /locus_tag="ACFI8B_001913"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC3835055.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(4434..5372)
                     /locus_tag="ACFI8B_001914"
     CDS             complement(4434..5372)
                     /locus_tag="ACFI8B_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835056.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            5574..6686
                     /gene="nadA"
                     /locus_tag="ACFI8B_001915"
     CDS             5574..6686
                     /gene="nadA"
                     /locus_tag="ACFI8B_001915"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC3835057.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6738..8246
                     /gene="nadB"
                     /locus_tag="ACFI8B_001916"
     CDS             6738..8246
                     /gene="nadB"
                     /locus_tag="ACFI8B_001916"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC3835058.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQA
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1832 a         2485 c         2364 g         1764 t
ORIGIN      
        1 cattcccgcg ctgcaatggg acatcggcgg cagcggggcg gttttccctt cgctcgcact
       61 gtttctgctc tgtttcatca taggtatgca caacacgggg atgacgcttc tgccgggcgg
      121 tgcaatccgt tcgacgcaca tggcccggca cggcagccga cttgggcatc gaaatcccgc
      181 gcgtgccgta ctatagtgga ttaacaaaaa ccagtacggc gttggcccgc cttagctcaa
      241 agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatctgtact
      301 gtctgcggct tcgccgcctt gtcctgattt ttgttaatcc actatattcc taaacccacc
      361 atccgcgcct gccgcacgta cacgtcgaca aaccgaaaat atggctgctc ggcggcatcg
      421 tcggcgcgtg gggctgccat aaaatcggac accatttctc cctgcccgtt tcggcagtat
      481 tgccgatacc gggtgcaggc tcggtcggct acgatgtgaa ggtacgtttt tttgcggatg
      541 tggctgcggt ttcggcgcaa atcacgataa acccgcgttg tttcgggaaa aatcgggtaa
      601 tttcaggtta attgcacttt ttcagacggc atgattgctt atagttgtac ggtttgacat
      661 atgcacggaa ggaaacgcca tgactttctt caaaccctct accgtcgtgc tgaccgcatc
      721 cgcactcgcg ctttccggct gcgttgccga ccccgtaacc ggacagcagt ccccaaacaa
      781 atccgccatg tacggtttgg gtggcgcggc agtgtgcggc atcgtcggcg cactgaccca
      841 cagcggcaaa ggcgcacgca attccgcgct tgcctgcggc gcaatcggcg caggcgtggg
      901 cggctatatg gactaccaag agcagcgttt gcgccaaaac cttgccggca cgcaaatcga
      961 aatccaacgc caaggcaacc aaatcaggct ggtgatgccc gaaagcgtta ccttcgccac
     1021 cggcagcgcg gcgttgggcg gcagtgcgca atacgccctg aacactgccg cacagacgct
     1081 ggtgcagtat cccgacacga cgctgaccat caacgggcac accgacaaca caggttccga
     1141 tgcagtcaac aatccgcttt cgcaacaccg cgcccaagcg gttgcctact atctgcagac
     1201 gcgcggcgtg gcggcttcgc gcctgacggt ttacggctac ggttcgcata tgccggtcgc
     1261 gtccaacgct acggttgaag gccgcgcgca aaaccgccgc gtcgaaatcc tcatcaaccc
     1321 cgaccaacgc gccgtcaacg ccgcacggca catgtaacgc gccctgcaac gccaatgccg
     1381 tctgaatgtt tttcagacgg catttccgta ttcgcaagca cagccgtacc ccgactgtaa
     1441 tgctatggat aacgtctcgc cgtttgccgg gaaaccgcag attgcagccg gtggcgtgca
     1501 agtgggccca accttgcaga cgttaccgcc ataccgcgat acccctgtaa cctcatcgtt
     1561 taaacagata ttcaatcaaa atgccgtctg aaaccctgcg aagtttcaga cggcatttgc
     1621 ctttcccgaa ttacaaatca aattctgccg ccacgggcgc gtggtcgctc ggacgctcca
     1681 gcgcgcgcgt ctccaaatcg atgcggacat ccttcaaaac ggccgccatt tcggatgtaa
     1741 ccaatatatg gtcgatacgc aggcccagtt tgcgttggaa catcgcgccg cgatagtcga
     1801 accaggtata gaacgcgcct tcgggatgga cttggcgcag gctgtcggtc aatcccaaat
     1861 ccagcaggtt ttgaaaccac tgccgttcga cggacgaaca gtggattttt tcataccatt
     1921 tttcagggtc gtaacagtcc gcatcggcag gcgcgatatt gaaatcgccc agcagcacca
     1981 gtttgccgtg gcgggacatt tcatcgcgga caaactccgt cagtgcggca aaccattgtt
     2041 ccttatattt gaatttgggg ctgtcgaggg cttcgccgtt gacgcaatag acattgatga
     2101 cgcgcacgcc gccgacggtc gccgcaatca cgcggcgttg cgggtcatcc ggcagggaag
     2161 gcaaaccgaa atgcacgtcc tgcggcacgc tgcggctgac gattgccacg ccgttgtagg
     2221 ttttctgccc gctccaaaca cagtgccagc ccatcatctg caaggcggcg gccggaaatt
     2281 tgtcctgatc gagtttgagt tcctgcaaaa ccaaaatatc gggcggattg tcggcaagca
     2341 ggttttgcac ctgcggcagc cgcacattga gcgaattgac gttccaagtg gtgattttca
     2401 taacatttcc gaaaaatgcc gtctgaaacg ttcagacggc aaaagaaaag gattctaaca
     2461 ctatgccgcg cgtttgtttt ccaaatcgcg gattgtccgc agaatcgttg ccgcgtcttc
     2521 ggaaaccagg cggtattcga taatgcggtg gtaacgcgta aagtccacca gaccgcccgc
     2581 cctcaggcgg ttcaaatgac aggaaaccgc ggtggccgtc agggataagg attttgccag
     2641 ttcgacgata ttgcgttcgc tgtccaacag ttggatcagt atcgccatac gttcgggatg
     2701 cgccataagt ttgagtatgg tgtggagcgg tattgtgttc atcggtattc ctaaaacctt
     2761 ttccgcccgc caagcggatt gacgggcggc agatatggat gattttgatt atctttataa
     2821 attttattat aaatcaattg tttatttctc attgtacagg attgtatgtt ccttttccca
     2881 ctatttttgc aaacaagcgt aaagacaggc tttcagacgg cataaaactg ccgcccctac
     2941 cctgcctcac cctgttgccg aacggaatca tccgtatttt ttaagaaaac cattatggat
     3001 attttccgtg aatcaaggcc gttctgcgcg acaggctgtc caacgccaac atccggttcg
     3061 gcatttcatc atcgccgccg acgatacggg caagaccgca tcggtcgtgc cttcctaacg
     3121 atgcaccgca tcctcgacaa ggaaaaccag atacataccg atatggcttt agcgttgccg
     3181 gcggtagtga aagacgcagt ggcgatatgg gcagatatgg atacattgga taggtgttcc
     3241 ggctgaccgc tccgttactg ccgtatcttt cggctaccgt tcatcaaatg gttccaatgc
     3301 cgaaatgcct aaatcgcgcc tgcctgaaat gctgtttttt ctaaagcatc acgaccttca
     3361 cgaaaaatgc cgtctgaaga atatttcaga cggcattccc tccctgaggc aataaccgga
     3421 ttacggttaa gcggttcctt acctgacata tcagacagcc gcccgcaccc tgaaactcac
     3481 gccacgaaat ccaaacctat gtccaacgat cggctgctgt gggtcagata gccgagggcg
     3541 atgccgtcca cgccggtttg cgccacgcgc ttcaggcggt cgaagccgat gccgcccgat
     3601 gcttcgcaat agacggtgtg tgggtgggcg gtttgtgtgt ggcagcggtt tgccgcttct
     3661 ttcagggttt cgtcgtccat gttgtccagc aaaatccgtt ccgcgcccgc tgcgatggct
     3721 tcgtccagtt gtgccaacgt gtccacttcg atttccacgc aggtcaacgg tccgaccgcc
     3781 tgttttgcct gccgaacggc ttgggcgatg ctgccgcaat aggcgaggtg gttgtctttg
     3841 atgagcacgg cgtcgtccaa acccatgcgg tggttcgcgc cgccgcccgc cctgacggcg
     3901 tatttttgca ggacgcgcag cagggggatg gttttgcggc tgcacacgat gtctgtaccg
     3961 tattcggcga cctcggcaac ggcacgagcg gtggcggtgg cgatgccgct caagtgcgtg
     4021 aggtagttga gcgcggtgcg ttcggcggcg agcagcgcgc gggcgttgcc ttcgacggca
     4081 gccagtgtct gacctgcgcg gacggcttgc ccgtcttgga tttcggcttg gaagcggacg
     4141 cacggatcca tcgtctgaaa ggcgaggcgc gccaagtcca taccggcgat aacaccgtct
     4201 tcgcggctga cgaggaaaag tttggcggtt ttgtcgggcg cgatgacggc ggcggacgta
     4261 atgtcgccgc gcctgcccaa gtcttcgctc aaggcttgtt ccaccatggg gcgcaacaag
     4321 gtgtcgggca gagcaaagag ggggttttca gacggcatgg ggtttccttt gttaaaaaag
     4381 attgatgttg caccgatggg gcaaaacttc atttgatgcc gtctgaaaac gggctaacgg
     4441 ctgcccagcg gcagtccgat gtccgaaatc agcctgtcgg acaggacgtt gtcgcggaag
     4501 cggtaaagct gcgcgggacg gcctttgctg cccgatacgc cggtatccga cggctcgata
     4561 aggttttgct gctgaatctg gcggcggaag ttttgcttgt gcagcaatct gccgctgatg
     4621 gcttcgacgc tgttttgcag ttgcagcagt gtaaatccgg gcggcatcag ttcgaaaatc
     4681 acggggcggt atttgatttt ggcgcgcagg cgggacaggg cggtcgccaa cacgcggcgg
     4741 tggtcgtggc gcatggtctg ccccgtgagc gcgaagtcga agtttgcctg cggctcggcg
     4801 gcctccgcta tcagaccgct ttcatacaac atttcatagc gttgcaaaac gtattcttcc
     4861 gaccagtttt ccggttcgac cccccaacac aaatgaatat gcttgagccg cttttggcgg
     4921 acttcctccg tgtccgccga gtttgcccaa atgcgcaggc ggctgacgac ggcgtcgcgc
     4981 tgcccgccgt cggtgcgcaa gtcttcccat gggaaatagc cgtagcagtc ctgccatttc
     5041 gcgtcgggat gcaggatgct atcggctgcc tcgcgcacca gccccaaata gctgacgtac
     5101 agcacgggca tgccgtgttc gttgcggcgg tgggtatcga caaaggtgta aagctgttcc
     5161 acatagccca taggctgcga agtctgcttg gcgacccaca gctttacgcc cgcctgcagg
     5221 gaattgcgca ggggggaaag cgggccgttg ggcaggagcg tgccttgggc gacggtcaac
     5281 acccgcaggc cgccgtcggt aacggcaatc aatacgggaa ccagctcgac gatgctttgc
     5341 agcggagctt cggcttcggg gtaggcgtcc atgctcggct ttctgcgggt gggaaaggct
     5401 ttattgtaaa ccaaagcggt ttgtcggaac gaagcgggtc ggttttgcgg tatcgcaaac
     5461 ctgttttcag acgaccttgc atattatact caagttgagc ataatgtaaa aacagtttga
     5521 aaaacagacg gcaaaccttc atgccgtctg aaaaccagct acaaggaaac atcatgcaaa
     5581 ccgccgcccg ccgctcgttc gattacgata tgcccctcat ccaaacgccg acttccgcct
     5641 gccaaatccg tcaggcgtgg gcgaaggttg ccgacacgcc cgaccacgag acggcaggtc
     5701 gtctgaaaga cgaaatcaag gccttgctga agcggaaaaa cgcggtcttg gtggcgcatt
     5761 attacgtcga tccgctgatt caggatttgg ctttggagac gggcggatgc gtgggcgatt
     5821 cgctggaaat ggcgcgcttc ggcgcggaac acgaagccgg tacgctggtg gtggcgggcg
     5881 tgcgtttcat gggcgagagc gcgaaaatcc tctgccctga aaaaacggtg ctgatgcctg
     5941 atttggaagc ggaatgttct ttggatttgg gttgtcccga agaagcgttt tcagcgtttt
     6001 gcgaccaaca ccccgaccgc acggtggcgg tgtacgccaa tacttccgca gccgtgaaag
     6061 cgcgtgccga ctgggtggta acgtcttcgg tggcgttgga aatcgtgtcg tatctgaaat
     6121 cgcgcggcga gaaactgatt tgggggcccg accgccatct cggcgactac atccgccgcg
     6181 aaacgggcgc ggatatgctg ttgtggcagg gttcgtgcat cgtccataac gaattcaaag
     6241 ggcaggaact ggcggcgttg aaggcagagc atcccgatgc tgtggtgctg gttcatcccg
     6301 aatcgccgca aagcgtcatc gaactgggcg acgtggtcgg ctcgaccagc aaactactca
     6361 aagccgccgt atcgcgccct gaaaaaaaat tcatcgtggc aaccgatttg ggcatcctgc
     6421 acgaaatgca aaagcaggcg cccgacaaag aatttatcgc cgcgccgacg gcgggcaacg
     6481 gcggaagctg taaaagctgc gcgttctgcc cgtggatggc gatgaattcg ctgggcggca
     6541 tcaaacacgc cctgacaggc ggacgcaacg aaatcctgtt ggacagaaag ctgggcgaag
     6601 ccgccaaact gcctttgcag cgtatgctcg acttcgcggc aggactcaaa cgcggggatg
     6661 tgttcaacgg catggggccc gcctgatttg ccgtccatat cgccgtttca gacgacctct
     6721 caaacaagga caacaccatg caaaccgatt gcgacgtatt gattgccgga aacgggctgg
     6781 cggcactgac gctcgccctg tcgctgcctg aatcgttccg catcgtcatt ttgtgcaaaa
     6841 accggctgga cgacaccgcc agccgtcatg cgcaaggcgg gattgcggcg gcgtggtcgg
     6901 gagaggacga catcggaaaa cacgtcgccg atactttgga agcgggcgca ggtttgtgcg
     6961 acgaagccgc cgtccgcacc atcctgtcgc agggcaaacc ggcaatcgaa tggctgctgg
     7021 cgcagggcgt ggcgttcgac cggaatcata acgacctgca cctgacgcgc gaaggcgggc
     7081 atacctgccg ccgaatcgcc cacgtcgccg actacacggg cgaagccgtc atgcagagcc
     7141 tgattatcca aatacgccgc cgaccgaaca tccgcgttta cgagcggcag atggcgttgg
     7201 acgttcaaac cgaatcaggc gcagcgtgcg gactgaccgt cctcgactgc cgaacgcaag
     7261 aaacctaccg catccgtgcc cgccataccg tactcgcagg cggcggcctc gggcaaattt
     7321 acgccgccac caccacgccg cccgaatgca cgggcgacgc catcgccatg gcgatacgcg
     7381 cgggttgcgc aatcgaaaac ctcgaattta tccaattcca ccccacaggc ttggcaaggc
     7441 cgtctgaaaa cggccgcacc ttcctgattt ccgaagccgt gcgcggcgaa ggcggcatcc
     7501 tgaccaatca atcgggcgaa cggtttatgc cgcattacga ccgccgcgcc gaactcgcgc
     7561 cgcgcgacat cgttgcccgc gccatcgccg ccgaaaccgc caaacaaacg caagacttcg
     7621 tctcgctcga catcagccgc caacccgcag cgttcgtccg tcggcatttc ccgtccatcc
     7681 atcggcactg cctgtcccaa tgcggcttgg acatcacgcg ccaagccatc cccgtccgcc
     7741 ccgtgcaaca ctacacctgc ggcggcatcc aaaccgaccc cagcggcaga acctccctgc
     7801 cgcagctcta cgccttaggc gaaaccgcct gtaccggact gcacggagcc aaccgcctcg
     7861 ccagcaactc cctgctcgaa tgcgtcgtta ccgccaggct tgccgcccaa gccatcgcgg
     7921 acggacaagc gttccaaatc gaaccattcc aaaggccgtc tgaaagcccc tccgccgaaa
     7981 cagacatctt ttcaaacgac ctccaaaaca cattcagccg ccctgtcctg caaacgttca
     8041 accaacgcca tctcggcatc ctccgcaacg ataccgacct gcaccgcgcc atcgaccaac
     8101 tgcggctttg gaagcaaaac caagccgaac cgcacaccgc gtccgaatac gaaaaccgca
     8161 acctactcga atgcagcctc gccgtcgccc aagccgcata caggcggaga cagaacatcg
     8221 gtgcgcattt taatagtgat tgttaaggtt ggctggttag aaaaaaatcg ttcaaataaa
     8281 tttgaatagc atggttaaga gattggcatt cagtttttat ggttgcgtaa gttgggttga
     8341 ggcctttgca aaaaagccct tccttcgaca gccgaaaccc aaacacaggt tttcggctgt
     8401 tttcgcccca aatacctcct aattttaccc gaatatcccc ttaat
//
LOCUS       DAXFWM010000081         7968 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000081 DAXFWM010000000
VERSION     DAXFWM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.081"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..3447
                     /locus_tag="ACFI8B_001917"
     CDS             <1..3447
                     /locus_tag="ACFI8B_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC3835059.1"
                     /translation="FSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVHGKRS
                     ADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAITPNI
                     RGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRGVKSG
                     VGGAMSIRTIEPSDIIPEGRNWGIEVKTEFSGNTVAQKNDLRQFLGRDYRTLSPIGAT
                     ADGVSGMPDVLTGYTGKPSPTALLLDEGIADTKFSGGKSHTNFKDDRQLMLSAAFKTD
                     ITDGLAAYSHRQKGNYYAGKRGYQSYLNNPIYGADACYDQYPDKSWREKDILCKSSAS
                     LVPNMAVLFRPGEEIMNSHTDTKILLLKNNWYLPDNQKISLQYMDNKIGFGEINPFHT
                     TYVMNMEEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIK
                     TNSTRHQSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLT
                     KEQVLAMTPNDNGQFRVISDAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLA
                     EEVEIVNSQDLFNLAGMVTGMTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYH
                     NFRGFDTALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQ
                     AYQSGDAAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVN
                     EAQNQPLYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAG
                     VGNPIDCIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQP
                     KPIRAHAWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNF
                     RFKPERSRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKD
                     TAGLEWQSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTR
                     GYQALQPKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGK
                     PHHWRSSLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTA
                     KF"
     gene            3662..4645
                     /locus_tag="ACFI8B_001918"
     CDS             3662..4645
                     /locus_tag="ACFI8B_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC3835060.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            4916..6406
                     /locus_tag="ACFI8B_001919"
     CDS             4916..6406
                     /locus_tag="ACFI8B_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3835061.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(6474..7871)
                     /gene="aspA"
                     /locus_tag="ACFI8B_001920"
     CDS             complement(6474..7871)
                     /gene="aspA"
                     /locus_tag="ACFI8B_001920"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC3835062.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         1917 a         2298 c         2132 g         1621 t
ORIGIN      
        1 ttctccccca aacctaagac cattatccta agccttgcag gtgcatttgg cgcattggct
       61 tttgcagaca ccccgaacaa taccgaacag cagaaagaac tgaataccat agtcgtccat
      121 ggcaaacgca gtgccgacca aaaaggcgcg gacgacgtgt attacaaaaa cgtctccaac
      181 gcctacgtcg gcaaagaata cctcgaacgc taccgcgtcc aatccgccgg cgacgtactc
      241 aaaggcctaa acggcgtgta caacatgaac acccgcaccg ccggcggcgc catcacgccc
      301 aacatacgcg gtatcaccgg caagggccgc atcccggtta ccatagacgg caccgaacaa
      361 accatcgacg tatggatgaa caactacggc gtgggcgacc gcaactacct cgaccccgcc
      421 ctgttccgca gcatcgcggt ggaaaaaagc cccgccctca cgcgcggcgt gaaatcgggc
      481 gtcggcggtg cgatgtccat ccgcaccatc gaaccttccg acatcatccc cgaaggaaga
      541 aactggggaa tcgaagtcaa aaccgagttt tccggcaaca ccgtggcgca gaaaaacgac
      601 ctacgccaat tcttaggccg cgactaccgc accctctcgc ccataggcgc gacggcggac
      661 ggggtatcgg gtatgcccga tgtattgacc ggctatactg gcaaaccttc ccccaccgcc
      721 ctgttgttgg acgaaggcat agccgacacg aaattctcgg gcggtaaaag ccacaccaac
      781 ttcaaagacg acaggcagct gatgttgtcc gccgccttca aaaccgacat tacagacggc
      841 cttgccgcat acagccaccg ccaaaaaggc aactactacg cgggcaaacg cggctatcaa
      901 agctacctca acaaccccat ttacggtgcg gatgcctgtt acgaccagta tcctgacaaa
      961 tcatggcggg aaaaagatat actctgcaaa tcttccgcca gcctggtgcc aaatatggcc
     1021 gtcctgttcc ggccgggcga agaaatcatg aacagccaca ccgacaccaa aatcctgctg
     1081 ctgaaaaaca actggtatct tccggataac caaaaaatca gcctgcaata tatggacaac
     1141 aaaatcggct tcggcgaaat caaccccttc cacaccacct atgtgatgaa tatggaagag
     1201 cacaacgaga ccaaccgccc caaagaactg tcgccacagg cgcagagcat cgattccacc
     1261 atccgcaccg acacctacaa actcggctgg gcctggaagc cggaaaacag ccgctgggtc
     1321 gatctgcagg ccaatctgtg gcgcatcaaa accaacagca cccgccacca gagtggcggc
     1381 atggatttgt cttccgcccg ccccgatccc ttctacgacg tttggtattg gtgtacccaa
     1441 cgcggccgga taccgcccga gcacgtcgac aactacagca gctgcaacga cctgatgaat
     1501 gatttcggcg tcaacggcct gaccaaagaa caggtgttgg ccatgacccc gaacgacaac
     1561 ggccaattcc gcgtgatttc cgacgccgag cagaaaaccc gcgtcagccg caccggtttc
     1621 gatatcagca accgcttccg cctcagagac cgcctgagca tgaccttggc cgccgattat
     1681 cagaaagaga aactggcgga ggaagtggaa atcgtcaatt cgcaagacct gttcaacctg
     1741 gccggcatgg taaccggcat gaccaagctg gccgggccgc gcggcggcga gcggcgcgaa
     1801 tggggcacta atttggtgtt cgactggcag gccgccgacc gcctgaaaat ctccgccggc
     1861 atccgctacc acaacttccg gggcttcgat accgccctgg cggaagggcg tgcccgccgc
     1921 gacccgcgct atcaggcagg cgatggaacc cagcattatg ccgatggtgc ctatctgccg
     1981 tatttcgaac tggcaggcga tcaggagcgg cgtgactgga atgtggtggc cgaacagctt
     2041 cggcaggcgt atcagtcagg cgatgctgcc gccattgccg cggcagtaca ggcagaacag
     2101 gcgcacggcg cccgttacca tttgctgaaa tactatgacg gctcgtatcg taccgatggt
     2161 tatgtgtata tcggcaacga tggccgtgcc tattttgacg gacggttcgg cggggttaac
     2221 gaggcacaga accaaccgct ctatcgggtg cgccccgttt atgtgccgtt tgtcaacggc
     2281 aagctggaca gcggcgcatt gccccagcat tttcatgatt tcgtcagcaa ttatcaggaa
     2341 aaagtgacca atccgcaggg gctgcacggg acgcattatc gatattgggc aggcgtgggg
     2401 aatcctatcg actgtataag aagtgatgcg gcctcttgcc ttgctcataa cctatcggga
     2461 aaagtaacca attttcaagt cggctatccc gagtacggtg atgataccgg tctcggtctc
     2521 aaaatcatcg gtcggcacta taccgaggaa cagtattggg cgcagcccaa accgatccgt
     2581 gcccatgcct gggcgccgac catcgccgtc agctacgatc tgaccgacaa cagccgcctg
     2641 ttcgtacgct atgcgcagat gagccgcttc cccagcgtgt atgaggtggg cagtttttat
     2701 aacgacgtgg cctatgccgg tatgccgaag gccccaaatt tccgcttcaa gccggaacgc
     2761 agccgcagct gggaaatcgg ctacagcttc aattttgccc cgtattggag ccggctgcgt
     2821 gccggcgata tgcgcctgac ctactaccgc aaccgtatcc aaaatgtgat cgaaaccaca
     2881 gattatttcc gcaccgtgca atacgaccgc aaggacaccg ccgggctgga atggcagagc
     2941 cgcatcgaca ccggccgctt ctttgccgcg ctcggtgcca cctaccgcct gaaacagcag
     3001 atgtgtgacc gcgataccgc tttcgattac gacccttatg gtgccaaagg cgtgccgatc
     3061 tgcatcgaag gcggctacgg cagtacgcgc ggctatcagg ccttgcagcc gaaatattca
     3121 ataaacctgg atgccggggt gcgcctgttg ggcgagaaac tggagctcgg cctgcgcggc
     3181 atctaccaca gccgggtcaa caccaagcaa tacgaccaac tgctgcaaaa ggatttgggc
     3241 atcatcttcg ataccaccgg caagccccac cattggcggt catcgctgac ttgggatgtt
     3301 tatggccgct accggctgca caaaaacctc aacgtcaacc tcggcatcac caacctgacc
     3361 aaccgctact atctcgaccc gatgtccaac gttgccgccc ccgggccggg ccggaccgtt
     3421 acctttgggc tgactgccaa gttctagccg cagggcggag tggggttttc aggtagcctg
     3481 cgggtatggc gggaaggcta cctgaaaacg gattggatat aacaacaaac aattgaacac
     3541 tccggacgca caaacccaac cggccccgct tatccgaggt aatggttttt accgtttttt
     3601 ccgccggttc ggcggtaata aaacggtggc ttttgtttaa ctcttaatta aggaaacgat
     3661 tatgtctata cgtaataaaa ccctgttggc cgtcttgctg tgcggcgttt ttggtgtggc
     3721 tgcggctgct ccggctggtg gttccagcca aaggaaaagc caagatccaa atagtgaccc
     3781 gacgacagtt cgccccggca cctctgacaa gcaacctttc ggtgccggta aggccggttt
     3841 ggcattcagt atcaataacg gtggaagttg gtcgaaatcc atcaatctgg atgatggtcc
     3901 ggtcaaatat atccgtgcga aatatcaggc accgattaac ggcaacgatc cctatgctaa
     3961 tgttgatggt acgcgcaatc tgcaatatct gcgcttaggc gatgtgaaac cggttatcgg
     4021 ctggttctac aacccaacgt tgggtcaggt gtggtatgaa aaccaccaca acaacactga
     4081 agtgtacagt gtgcgccaga tagccaaccc ggcaatcccc gccgcaccca agttcggcgg
     4141 cctggtaatc gccaaagtgc cgggcatggg cggcagcaac ggcaacgtct tcttcggcga
     4201 atgggcgccg cgtgccggta acccgcagca aatcagcacc gacctgaata tggcggatgg
     4261 taaacgcacc gtttggtatg tgggcgataa tcccaccggc aacaccaccg gtgtgagaac
     4321 cgccacctac aatgtgctgg gtatcaataa gcacaccccg gggcagaacg atttctatac
     4381 cggtcaggta accgccacct tcggttctgg tataaccggg acgatggcag gcagaatcag
     4441 ccgcagttcc gatcacgtcg attttgatca cgccaaaatc gataacagaa atggtacctt
     4501 tgccgatccc agtcaaggca tgaccggccg cttctacggc aaccaggcgg cagccatggc
     4561 cggctatacc acacgtggta ccggtaatgt cggcgatgac gtagctttcg gcggtagcgc
     4621 caccgatatt gagacccgca aataacagct gccatccgat ttggtttggc aacggctagg
     4681 ctcgatttaa cccctgagga gtttcaagct gccgttttat gccgactgcc aagccatata
     4741 aatcatccgt ccgatacgga ttgctgctcg gtttcaggta gcccgtgttc aacacaggct
     4801 acctgaaatt tgatatttgt aaataaacat cgcctatccc atacggtttc ggcctatcgg
     4861 acattttggg acagcaatta aaacctgcgt tactttatct tttcggaagg caaccatgaa
     4921 caaaccactc agcctcgccc tcttctgcct gcttctgccc atcaccgccc acgccgacaa
     4981 ccccgccgac gaacgccgcc gcctgctgga cgagggcagc cgccaaacgc agcaataccg
     5041 cgaaagcggt tggctggaca cggaacaggc acgcggggaa gtagaggaaa acgacggcta
     5101 tatttcgata ggcggcgaaa tctaccaagt cggcgatacg gcggaagaac tggaaagcgc
     5161 aatttaccac gccctcaatg cgcggcagtg gcacaaggtg cggcagttcg ccgcccgcta
     5221 cgccaaactg ccccggcaca agcccgcgct gattcatttg gccgatgcgc tgcaaaaacg
     5281 ggacgaaggg gatttccgcg cggcggggaa cagttttcag acggccttgg aagccgaacc
     5341 cgacaacccg cgcctgctgc tcgaagcggg gcggttttat gccgaagaca accaaaacaa
     5401 agagtctgcc gccgcgtttg agaaagttct gaaaacggac attcccgcag aaacccgccc
     5461 catcgtggaa aactacctgt ccgaactggg caaacgccgc cgctggcacg gccaaatcag
     5521 cctcggctac ggctataaca gcaatgtaaa ccaaggcaac ggcatcaacc aatgcgtgtg
     5581 ggaaattgcg ggcatgtgcc tgatggagcg caccctgccc gcgccaacgg attcgacctt
     5641 ctcatcctat agcgcgaccg ccgaaaaaac cgtcccgctc aaagggcatc acggcgtaca
     5701 ggtgcgcggt gtactgtacg gcaaccgcta cacggaaaaa gacaaagatt cggcggccat
     5761 gccggattac ggctatcgca acggttcgct gtacgcggga tacgcctatg ccgacgcacg
     5821 ctcctccttt tccctgctgc cttatttcga atacgacttc cgcaaccgcc acacccacta
     5881 ccgcgcatgg ggtgcggatg ccgactggtc gcgcacactc tccccgcact ggcgcattaa
     5941 ctcacatgcc ggagcaaaaa agaccggata cggcggacaa agcaaaactt attttgccga
     6001 tttcaaacaa tacgaactcg gcgcgggggc ggaattttcc atcacgccga aaagcggcct
     6061 gcttgtaaat ttcgatgccg cacgcaaagc ctatcccgaa aaatcctctt ccagcaaaga
     6121 atatacggct cggctgggcg cgtataggct tttttcgggc ggtacttatt taaacgccgt
     6181 cctgctctac cgccgcagcc tgtatgacgc ggcaagtttc gtcagcgata acaaacggcg
     6241 gagcgacaag caatacatca tgatggctgc ggcgggtttt ccgcaatgga atatcaaagg
     6301 cgtatatccc gaactgcgtt tcaggcgaac aatcgcacac agcaacgcgg tgtattaccg
     6361 ctaccgccaa aacgaatggc tgttgggttt taaatatcgg ttttgaccag tcgggtgata
     6421 aaaaatgccg tctgaaaggg cgcttccttt cagacggcat ttgtccgtaa tgtttacaga
     6481 tgaggattca tcaggttctc gggggagagg atgcggttga gttcttcttc gctcaacaac
     6541 ccgcgttcca gtacgacttc gcgcacgcct ttgccggttt gggcgcagat tttgccgacc
     6601 aagtcgccgt tgcggtggcc gatgtacgga ttcagataag tcaccagccc gatggagttg
     6661 aaaacgtaac gttcgcagat ttcgcggttg accgtaatgc ctttgacgca tttgtcggat
     6721 aggttgactg cggcattgcc caagagggaa atggtttcaa acatacattg ggcgatgacc
     6781 ggctccataa cgtttaattg cagttgcccg gattcggcgg cgaaggtaat cgtcgtatcg
     6841 ttgccgatga ctttgaagca gacttggttg acgacttcgg gaatcacggg attgactttg
     6901 gcaggcatga tggaagaacc ggcctgcaat tcgggcaggt tgatttcttt caaaccggcg
     6961 cgcggaccgg aagagagaag gcgcaaatcg ttgcagattt tagagagttt gacggccgtg
     7021 cgtttcaatg cgccgtgtac catcacatat gcaccgcagt cggaggtcgc ttcgatcagg
     7081 ttttcagtca gtttgcaagg caagccgctg acttcggaga gttttttgac caccagttcg
     7141 gcatagcctt tgggcgtgtt cacgcccgtg ccgattgccg ttgcgcccaa attgacttcc
     7201 aagagcagtt ggcgggtgcg gtcaaggttg aggatttcct cttccaacaa tacttggaaa
     7261 gattggaatt cctggcctgc cgtcatcggt acggcatctt gaagctgggt gcgacccatt
     7321 ttcaaaacgt ctttaaacgc ttcggctttg gcggcaaagg cgtttttcaa tacggtcagt
     7381 ttgtcgagca attcgccgat gctgtaatac acggcaaggc ggaagcccgt gggataggca
     7441 tcgttggtgg attggctggc gttgacgtga tccatcggat tgacgatgtc gtagcggcct
     7501 ttctcatagc ccaaggcttc caatgcaagg ttggcgatga cttcgttggt gttcatattg
     7561 accgaagttc ccgccccgcc ctgatacacg tcggacggga attggtcgag gcagcggttg
     7621 ttcagcagaa cttcgtcgca agccttttca atggcggcgg cgatttcggg ttttactgca
     7681 cccaattcgc cgtttgcctg cgcggttgct tttttcacca tcaccatact gcggacaaac
     7741 tgcggcacgt cggaaatttt ttgtgtggag attttaaagt tttcaatggc gcgcagggtg
     7801 tggatgcccc aatacacttc ggcgggaatc tcgcggtcgc ccaataaatc gtgttcgata
     7861 cggacagtca tgtttttacc tttgtaagtc ggataattaa tattgaaaaa atgcgccatc
     7921 ggaaagatgc cgcagcagga cgaacactat accggccgga tgaaattg
//
LOCUS       DAXFWM010000082         7921 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000082 DAXFWM010000000
VERSION     DAXFWM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.082"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(34..333)
                     /locus_tag="ACFI8B_001921"
     CDS             complement(34..333)
                     /locus_tag="ACFI8B_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835063.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            complement(333..1634)
                     /gene="nuoF"
                     /locus_tag="ACFI8B_001922"
     CDS             complement(333..1634)
                     /gene="nuoF"
                     /locus_tag="ACFI8B_001922"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC3835064.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            complement(2024..2497)
                     /gene="nuoE"
                     /locus_tag="ACFI8B_001923"
     CDS             complement(2024..2497)
                     /gene="nuoE"
                     /locus_tag="ACFI8B_001923"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC3835065.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(2497..3753)
                     /gene="nuoD"
                     /locus_tag="ACFI8B_001924"
     CDS             complement(2497..3753)
                     /gene="nuoD"
                     /locus_tag="ACFI8B_001924"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC3835066.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(3743..4336)
                     /locus_tag="ACFI8B_001925"
     CDS             complement(3743..4336)
                     /locus_tag="ACFI8B_001925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC3835067.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(4349..4831)
                     /locus_tag="ACFI8B_001926"
     CDS             complement(4349..4831)
                     /locus_tag="ACFI8B_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC3835068.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(4822..5178)
                     /locus_tag="ACFI8B_001927"
     CDS             complement(4822..5178)
                     /locus_tag="ACFI8B_001927"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC3835069.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            complement(5639..7090)
                     /locus_tag="ACFI8B_001928"
     CDS             complement(5639..7090)
                     /locus_tag="ACFI8B_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFC3835070.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLPPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(7113..7733)
                     /locus_tag="ACFI8B_001929"
     CDS             complement(7113..7733)
                     /locus_tag="ACFI8B_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC3835071.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
BASE COUNT         2068 a         1986 c         1998 g         1869 t
ORIGIN      
        1 caattaatta tatttacatc gccatgcaat atcctactgc atcgcaacta ccaccccgtg
       61 tccttgacgt gccaaaataa aatcacaatt atgcgaataa agtttgtata aatttctcct
      121 aacctcgtat tcattatcac cagatgaata aaaaccaatc ctctgacctt catgatcaaa
      181 tactgcaaca aaatctaagc cgtttccata tctttcaaaa aagactagaa atttagaaaa
      241 tttccgacaa atctgattaa aaagcgtatt gagtgtcgta ctcttacctt tgttggctgc
      301 accgtataga ataaaaattt tagcttccac cattaccacc ctccccactt atgctctttc
      361 atcggcccgc cgtgttcgat gtaatgcgca aactcatcac ggaaatgctt ggtaaagctg
      421 cggacaggga agacggcggc atcggcaagg gcgcagatgg tgcggcctgc catttggttg
      481 ccgacagaat ccagcaaatc caagtcttcc atgcggcctt tgccttctac gatgcggcgg
      541 acgatgcggt aaagccagcc ggtaccttcg cggcaggggg tgcattggcc gcaggattcg
      601 tcgtagtaga aatagcttaa gcgttcgagg gctttgacca tgcacacgtc ttcgtccatc
      661 acgataatcg cgccggaacc gagcatcgag ccggctttgg aaatcgagtc atagtccata
      721 ttggtctgca tcataatgtc ggcaggcaat acgggggcgg acgaaccgcc gggaataacg
      781 gctttgagtt ttttaccgcc gcgcataccg cccgccattt tcaagacttc ggcaaacggc
      841 gtgcccaacg gcacttcata gttgcccgga cgctcgacat ggccggaaat acaaaataat
      901 ttggtaccgc ctgcattcgg aataccttta tcggcaaacg cctgtccgcc gtcacggata
      961 atgaatggaa cggaggagaa cgtttcggta ttgttgatgg tggtcggttt gccgtacagg
     1021 ccgaacgagg cggggaacgg cggtttgaag cgcggctggc cttttttgcc ttccaacgat
     1081 tcaagcaatg cggtttcttc gccgcaaata tatgcgccgt agccgtggtg ggcgaagagt
     1141 tcgaattcaa aatccgaacc caaaatattt ttacccaaaa agcctgcggc acgcgcctgc
     1201 tccaaagcgg cttcaaagcg ttgataacct tcgaaaattt cgccgtggat atagttgtag
     1261 ccggctttcg cgcccatcgc gtaaccggca atgatcatgc cttcaatcag ggcatgagga
     1321 ttgaacatga tgatgtcgcg gtctttaaat gtacccggct cgccttcgtc ggtgttgcaa
     1381 accacatatt tttcgcccgg gaaagaacgg ggcataaagc tccatttcaa accggtcggg
     1441 aagcccgcac cgccgcgccc gcgcaagccg gaggttttga cttcgtcaat cacatcggtt
     1501 tgcgagatgt tttcggacag gattttacgc agggcggtat agccgccgcg tttgacgtat
     1561 tcgtccaatg tccagcaatc gggattggcg gtatccactt ggtcaaaaat cacgcctgat
     1621 tggtaaatag ccatttttgg tgtgcctgtt tgttttcgta tcggttgcgg ccgctgtttc
     1681 agacgacctt aaaatgtctt tgtgtatcgg cttgtaacgt cttctgaaat aaaatctagt
     1741 ttatcaaatc gatattttca gacgaccttc aatcgttttt gttggatctc gaccacttgc
     1801 ttgtctgccg tcattcccgc gcaggcggga atccatcctc aatggtaagc aatgtcttat
     1861 taaattcaga aaccgaatct taccggtgga ttcccgcctg cgcgggaatg acggcatttc
     1921 ggtatttcag tagggcggat tcttaaatcc gacattttgc ctttttaccc actctgtcgg
     1981 atacaagtat ccgacctacg tttaaatcgt cgtttcagac gacctactcc aactccgcca
     2041 gtttcttctc aatcgcctct tcggtcataa agctgcacat gctgtggttg ttgaccagca
     2101 taacgggggc atcgccgcac gcgcccatgc attcgccttc gacaagggta aatttgccgt
     2161 cgggcgtggt ttcgccgtag ccgataccga gtttttgttt gaggtattcg ccggtcgcca
     2221 taccgccgcg cagagcgcag ggcaggttgg tacaaacggt cagtttgtat ttgccgacag
     2281 gctcaaggtc gtacatattg tagaaagtgg cgacttcata tgcctgcgcc ggcgtgatgc
     2341 cgatgtagtc ggcaacaaaa gcgatggttt cgggggcgag ccagcctttt tcggtttggg
     2401 cgatacgcaa cgcgcccatg atggcggaac ggcgttggtc ggcgggatat ttcgccaact
     2461 caatgtcgat ttgttttaag gattttgcgg ataacattat cggtcaacct ccccgaatac
     2521 gatgtcctgc gtaccgatga tggcaacgac gtcggcgagc atatggcctt ttgccatttc
     2581 gtccatgcct tgcaggtggg cgaagccggg tgcgcggatt ttcaggcggt agggtttgtt
     2641 tgcgccgtct gaaatgatgt aaacgccgaa ctcgcctttc gggtgttcga cagcggtgta
     2701 ggtctcgccc tcgggaacgt gcataccctc ggtaaagagt ttgaaatggt gaatcaggtc
     2761 ttccatacct gttttcattt cggtacgttt gggcggagcg aatttgtggt ttgcggtaat
     2821 gaccgggccg ggattgacgc gcaaccagtc ggcgcattgt ttgatgatgc gtacggattg
     2881 gcgcatttct tccatacggc agaggtaacg gtcgtagcag tctccgttga cgccgacggg
     2941 gatgtcgaaa tccattttgt cgtacacttc gtagggctgt gtcttacgca cgtcccattc
     3001 cacgccggaa ccgcgaagca tcacaccggt aaagcctttt tgcatggcgc gctcgggaga
     3061 gacaacgccg atgccgacgg tacgctgttt ccagatgcgg ttgtcggtca ggagggtttc
     3121 gagtgtgtcg atatttttgg ggaagcgttc gcagaaggca tcgataaagt caagcatcgt
     3181 gccttcgcgg gattcgttga gctgcttcaa tactttggca ttgcggaatt tgctgctctc
     3241 gtatttgggc ataaagccgg gtaggtcgcg gtaaacgccg ccgggacgga agtaggcggc
     3301 gtgcatacgt gcgccggaca cggcttcgta caagtccatc agctcttcgc ggtcgcggaa
     3361 ggcgtagagg atggcggtca tcgcgccgat gtcgaaggcg tgcgaaccga tgcccatcaa
     3421 gtgattgagg atgcgcgtta cttcggcgaa catcacgcgg atgtattggg cgcggatagg
     3481 cacatcgata ccggcaagtt tttctaccgc caagcaatac gcctgctcgt tgaccatcat
     3541 ggaaacgtag tccaaacggt ccatataggg cagggcttgc agataggttt tggtttccgc
     3601 cagtttttcg gtgccccggt gcaggaggcc gatatgcgga tcggcacgga cgatttgttc
     3661 gccgtccagc tccaaaatca tacgcaatac gccatgcgcc gcagggtgtt gcgggccgaa
     3721 gttgatggtg tagtttctta atttattggc caccgtagtt ctcctcacgg acgatacgcg
     3781 gtgtgatttc gcgcggctca atggtaacag gttggtaaat cacgcgtttt tgctcttcgt
     3841 cataacgcat ttccacatag ccggaaatcg ggaagtcttt gcggaacgga tgtccgacaa
     3901 agccgtaatc ggtcaggatg cggcgcaagt cgggatggtt gttgaacatg atgccgtaca
     3961 aatcgaaggc ttcgcgctcg taccaatccg cgctgttgta aatatcggct acggattcga
     4021 ctacagggaa gtcgtcgtct gaaacccaga cgcgtacgcg gatgcgttga ttgtttttaa
     4081 cggaaagcaa ctgactgaca acggcaaagc gtttgccctg ccatgcttcg tttttgtaag
     4141 tgctgtaatc gacgccgcac aagtcaacca gaagctcgaa atgcaaatct tcatggtcgt
     4201 gcaatgcggt catgactgaa atatagtgct cgggcagaca ctcgacggta atctcgccca
     4261 aagctgaaat gactttgcct gcctgatcgc caagcacgcc gacgacggtc tcgtataagt
     4321 tttgaatgct tgccatatcg tcctctcctt actcgtcacg cgcgatggtg gaagtgcgct
     4381 tgattttttg ttggagctga atcaggccgt aaatcagagc ttccgcagtc ggcggacaac
     4441 ccggcacata aacgtccacc ggcacgacac ggtcggcacc gcgcacaacg gaataagaat
     4501 agtgataata gccgccgccg ttggcacacg aacccataga caatacccag cgcggctcgg
     4561 cgagctggtc gtacacgcga cgcagggctg gcgccatttt attggtcagc gtacctgcca
     4621 caatcatcag gtcggcctga cgtggggacg gacggaaaat gataccgaaa cggtcaaggt
     4681 cataacgcgc catacccgca tgcatcattt ccacggcgca gcaggccaat ccgaaagtaa
     4741 ccggccacaa tgaaccggta cgcatatagt tcaacaccgt atccgcgctg gtggtgatga
     4801 aacctttttt caaaacgcct tctattccca ttccagcgca ccttttttcc attcgtaaac
     4861 aaagcctacc gtcaggacga cgataaacac cagcatagac caaaagccgt acgcgcccaa
     4921 atctttgaac acgactgccc acggcagcat aaacgcaacc tccaaatcga agaggatgaa
     4981 caggatggcg acgaggtaat agcgcacgtc gaacttcatc cttgcgtttt caaaggcttc
     5041 aaaaccgcat tcgtaaggcg cgtctttttc ggcatagtgg cgtttcgggc ccaaaatcgt
     5101 gccgagcagg ataaacagta cgccggccgc gaggccgacg aggataaaga caaagacggg
     5161 aaaataagcg gacaacatgg ttacacccaa atccgttaac aaaatttcta caataatttc
     5221 gtattttagc gaatttcaaa aaccattaaa aggtaaatat cggcaaaacg cccaaaaaaa
     5281 cccaataaat acaatcatgt tatgataacg attcttattt gattttataa ggtcatataa
     5341 atttttacac cgtaattcgt ggtatagcgg attaacaaaa accggtacgg cgttgccccg
     5401 ccttagctca aggggaacga ttccctaagg tgcccaagca ccaggcgaac cggttccgta
     5461 ctatttgtac tgcctgcggc ccgccgcctt gtcctgattt ttgttaatcc gctatatata
     5521 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
     5581 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
     5641 aaatccctct gccgtatttg tattctacaa tcatattatc gtcagtaatg acttccgccc
     5701 cagccgaagg ttccgtcatc cgaatcagca tacgagagac aaccttttgt gctgcagcat
     5761 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggaaaggc
     5821 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
     5881 gcccgtagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
     5941 gcgtggtatt aaacattaca ataccatccg gggtaaggtg gctttgcacc tgttttaaaa
     6001 attccgcact caacaggtta gtggaatagg cacgccagta ccaagtcgaa ttcatcaaaa
     6061 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
     6121 tttcaacacg tttgtcctgc aaaagcggtg cgatttgcgg ctcgtccgcg ataaggctac
     6181 ggtatgccgg attgatttcc gcaacgatca tcgactgcat ttccggaatg gcagacaaga
     6241 cgcgcgccca cgaacctgta ctcaatccaa cgacgaaaat gcggcgtatg ccggacttca
     6301 gggagggtgg cagataggca cgttcgatgc cgttgacact attgaatata tcggtattgt
     6361 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
     6421 caacaatgcc gtgtttgttt tcaatcaacc tatccggacg gccagcaata ttttgaaaga
     6481 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     6541 cattcagtcg gagacttttt tggaacagtg tacaaaacaa agggacagca gcagaaatca
     6601 aacagatgag caggtaaatc tgttgggtgg acaagaaatc aagtatcaca aagccgataa
     6661 ggaccggacc caatgcactg ccggcaacgt tggcgaaata aacattggaa acctgtcgtc
     6721 cggatttgtt gccatccgta cccacatggt gtacaagtgg gaaaatcaac cccctgacga
     6781 cggcagacag ggtaatgaag ataccggcgt ggtggacgaa gccggaaaaa cccgtcaaca
     6841 accacgcagc acccaaaatc aaaaaatcgg caatacccgc ccacaagaag cactgcccga
     6901 taaagggaat atcaacaaag cggctgcggc aaatccgttt gccaaaatac gcgccgacgg
     6961 cgataccggt cagaaaacag gcaagagtaa atgaaaatgc ctgaggcacg gactgtgctg
     7021 cgaacgaaaa catccttacc cacaagactt ctatacccaa gctcaataat ccgctaagga
     7081 aagaaagcat ataaatcaaa ctagtattcg gtttagtgtt caccatatcc atcctttctg
     7141 taacgcagcc atactgaagc agcaatcaga aggttaaggc aggctgtcag cgcaatggtt
     7201 tgggagaggg taaaaaagac gtagaaaaat tcggcggcgg caagcgatcc gagtgccgca
     7261 cccaaagtgt tgaaaaaata taaggtaccg atagactcgc caacattatg tattttccgg
     7321 ttaaaaaaac aggtcagcaa gggcaaggtc gcgcccatca taaaggtagg aagcagcaat
     7381 aagaggaaat tggcagcagc gatgatgggc aaatcagcct caactaaaag atgccccaag
     7441 ccggaaatca gacccttgct taccaaaccg aacagaccga tggatacttc agcgatgcaa
     7501 aacaggggga tgatacttga aggaaaacgg tcagcaatgc gtccgccgaa atacgcacct
     7561 acacccaagc cgaccataaa tacagaaata atgacagtaa tcgaactcaa atcgatacct
     7621 atgtggctga atagaagcct ctgccagctg acctggtaaa tcagggcgca gaagccagag
     7681 gcgaaaaaca ccagcgacaa tcctttcagg gttttacttg cggtcgaatt catattttct
     7741 attttcaaat aggcggaact ataaatgaaa tgtattaatg tgtaaacatt tgtattagcc
     7801 gtaggaaatc accgcattgc tcaaactgca ggccgggaaa ataaactcgt aagcccgcaa
     7861 acggcgtatc actgttcaaa ctgctgtgcg gcttcatgac gttataaaaa ttaaggggcc
     7921 g
//
LOCUS       DAXFWM010000083         7706 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000083 DAXFWM010000000
VERSION     DAXFWM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.083"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            72..833
                     /locus_tag="ACFI8B_001930"
     CDS             72..833
                     /locus_tag="ACFI8B_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835072.1"
                     /translation="MEMQLDKQTLTVVVRNSEPVELSVFAQSMMSLADDYESMCGSAG
                     SHARLYIKEIRQGSIIAELAPLLPLAGTLFEGAGQILAYAKNFIEITDWLMGKGKEPA
                     GVTDSQIRNIANIMQPAASDKNGSIEINVNDNNGSVVNNITYNYFAANTVQNQARRIL
                     GERAEASESGDYGQMVMYFVQAAPTKETNQAVIEGIYSRPVKILIPEHIKREMFAEPY
                     PFEKYYIVDVSVQTARGKPRLYKVTGYHGVVDGDD"
     gene            complement(932..1333)
                     /locus_tag="ACFI8B_001931"
     CDS             complement(932..1333)
                     /locus_tag="ACFI8B_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC3835073.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(1435..1617)
                     /locus_tag="ACFI8B_001932"
     CDS             complement(1435..1617)
                     /locus_tag="ACFI8B_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFC3835074.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1787..2605)
                     /locus_tag="ACFI8B_001933"
     CDS             complement(1787..2605)
                     /locus_tag="ACFI8B_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFC3835075.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVAHSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2602..3300)
                     /locus_tag="ACFI8B_001934"
     CDS             complement(2602..3300)
                     /locus_tag="ACFI8B_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HFC3835076.1"
                     /translation="MDRAEQGVTSPFICMAENGLEYFVKGLHATRASQINEWIGGNMA
                     QALGLPVAPFELLEVGEELYEELPAKMKEIGKGICFGSLAQKGCALLEPADIPRIDTV
                     MQRQIAAFDWFVRNEDRTIGNPNLLYRNCNNSLIVIDHNCAFDTGFNPNNFLQNHIFS
                     SAFRQILEDWVLREEMELWLKSALPAYRKACDNLPPEWAWANEERDLPAAYNRSRTDE
                     TVRRIDNGTLWSIS"
     gene            complement(3539..4255)
                     /locus_tag="ACFI8B_001935"
     CDS             complement(3539..4255)
                     /locus_tag="ACFI8B_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC3835077.1"
                     /translation="MEKHETVIRLFDAAKTKGKNTPAETARLLNISQQTLKNWESRGI
                     SAKALPEVAQVLGVSETWLRTGEGSRTAPVLINPDLPHEVKDIHRPMMWSSNDPLPDD
                     DYVFVPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDE
                     TASLDSLTVIGRVFWWSVLD"
     gene            4636..4863
                     /locus_tag="ACFI8B_001936"
     CDS             4636..4863
                     /locus_tag="ACFI8B_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3835078.1"
                     /translation="MNQKQTQCKQIVDYIRKKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPKYKVGNCKNPVAHYSIAKSGIEP"
     gene            4981..6045
                     /locus_tag="ACFI8B_001937"
     CDS             4981..6045
                     /locus_tag="ACFI8B_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835079.1"
                     /translation="MMKPSESLRAAGRPIAYYPKLAKPLGGVNAAILFGHFFYWNDKT
                     QYESGIYRTAEEIEIETGLSVQEQRTARAKLRERGVLIETEKRIEHRIYYKLNLDAFD
                     DLMLQHSGGGEPTAPKCNINSPELQNQHSGGGESTAPKCNINSPELQNQHSGSEESTA
                     VIRTEDLTEDLAVYTPLPPNAGNGKGGLNADAFVSADAETCGRETGEPTSPKAESDSN
                     GNGGLSGKPKNANVPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRW
                     CEMLGTAAPNGKVRFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGN
                     FVKILEWHPPKTNQAARGRA"
     gene            6042..7403
                     /locus_tag="ACFI8B_001938"
     CDS             6042..7403
                     /locus_tag="ACFI8B_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFC3835080.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
     gene            7440..7703
                     /locus_tag="ACFI8B_001939"
     CDS             7440..7703
                     /locus_tag="ACFI8B_001939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835081.1"
                     /translation="MQPCRLQNQHRHADARFCEMREGAECGGKSDVLPANQSVRRRGV
                     SDGIGGGSRKKDGGAWGISPRNAPNLSGKAGKTLWEYPSLPEI"
BASE COUNT         1933 a         1806 c         2150 g         1817 t
ORIGIN      
        1 cgcaggcgca aaaaaaccgc ccgatatttc gggcggcgta ttaaaatcag atgaaattaa
       61 ggaggcttat tatggaaatg caattggaca aacagacatt gacagtggtt gtcagaaaca
      121 gcgagccggt ggaattgtcg gtatttgcgc agtctatgat gagccttgcc gatgattacg
      181 aatccatgtg cggcagcgcg ggcagtcatg cgcggctgta tattaaggaa atcaggcagg
      241 gcagcattat tgccgagctt gcacccctct tgcctttggc gggtacgctt tttgaaggcg
      301 cggggcagat attggcgtac gccaagaatt ttattgagat taccgattgg ctgatgggta
      361 aaggcaaaga acccgcaggg gtaacggaca gccaaatccg caatattgca aatatcatgc
      421 agcctgccgc atcggataaa aacgggtcga ttgaaatcaa cgtgaacgac aacaacggct
      481 ccgtcgtcaa taatattacc tacaactatt tcgcggcaaa cacggtacaa aatcaggcaa
      541 ggcggatact gggcgaacgg gcggaagcgt ccgaatcagg ggattacggg cagatggtga
      601 tgtatttcgt acaagccgcg ccgacaaagg aaaccaatca ggcggttatt gaagggatat
      661 acagccgtcc ggtcaaaatc ctgattcccg aacacatcaa gcgcgaaatg tttgcagagc
      721 cttatccatt tgaaaaatat tatattgttg acgtaagcgt acagacggcg cgcggcaagc
      781 ccaggctcta caaagttacc gggtatcacg gggtagtcga tggggacgat tgaaaaccgc
      841 cttttcgggc ggctttgtcg gttttgtgtt gttttcaggt tcggcggggc gtgaaaaaag
      901 cccgcgtgtg cgggcgggtt gaacgggctg gctatgcctg attcatggta agcagggcgg
      961 ctttggctaa aaaaccgcta cgggtttcat gatttgcgga tgtatattca tccactctat
     1021 caagaaggta ttgcggccag ctgacgttga atcggatttg ctgccgactg atttttgcag
     1081 ggtcgatttc aatcattacc catgttgccc catgataatc aggttcttgg ctcaaatcgg
     1141 caatgctgct tacggcaagg tttttgaacc ctccgtcctc aatcataccg tcaatatgca
     1201 tataggcggc agaacgggcg ttggcgacgg cttcttcaac ggtgtcgccg caagagaaac
     1261 agccgggaag gtcggggatg gttacgccat atgccgaatg ttcgtctttg tgcagggcgg
     1321 cagggataaa catttgattt cctttaatta tgttgttcag gtttatgggc gcgtgagata
     1381 acccgttgat tgtttgatta gaaggggggc agcgggaaac cccgctgcct gccgttactt
     1441 caaaccggct tgcttataga tattttttac agtacctgtc ggcaagtctt ttttcgggtg
     1501 cggcacggtt acacggcctt tttttgttgg gtgcttatat tgcgaatggc tcccggattg
     1561 tgcaacttta taccaaccgt cttgtttgag tagggcaata acgtctaggc tattcaatta
     1621 tcactcctaa tcttaatgca tggcacgcct gccaatgtgc gtaataatac acactaattt
     1681 ttattttgtc aatttttatg tgtatttgtg tgtaaaaata tctttcggca gtagttgcag
     1741 ggaagggtgt aaaaagcccg cataacgcgg gcggtcttga gggctgtcag ccgcaggcaa
     1801 agtttctgat gattttgcct tcgcttcggt tatcggcaag ccgtatgccc tgttcctcca
     1861 ggtcgcccaa tacgtcaagc aatgcctttt cttgtgcctt gtcgggacgc tccggcggtt
     1921 cgtaggcaaa caatatttca gagcggtcga tgtatccgcc gcgccgcaac cgttttatgc
     1981 cggcaatcca cttatcagac ttgtaataga tgtcggcagg gtctttctgt ccgaaataga
     2041 taggcttgat gattttccgt atttcgccgt tttccgcctt ttgcaccaag gggatggagg
     2101 cgtgaaagcc tgacgggtcg ccgattgtgc tttctttgaa ggggtatgcc gtctgaagcg
     2161 gctttaacat tgcctgtatt tgtcgggtaa gttctgcttc gggttggctt ttagcaaagc
     2221 tgtgggcgac aaaataatca aacaggcggt tcagttcctg ccccctgtcg ggggcgaggg
     2281 ttacgcccgg ttgggcggcc ataatcaggg cttcgcgcgg gcgggtcaaa tggtcgagca
     2341 tggcgcgtat ctgatcgggc gcggagtatg ccgccaattt cccaatccgc tgtaattctt
     2401 ctgcaaaggc acgggtcgcc gctttataga cggacgaatc gaagcggcgg aaaaaacggc
     2461 tcaatctgct gtaacgctgt tcgattttaa agtcgaagta gccgcttttg ggatgggtta
     2521 tgatgatgcc gatgttggca aattcgcgcg tttggacata gggcataaac cgtatgacgg
     2581 caaaacgcat ggcatattgg ttcatgagat gctccaaagt gttccgttat caatgcggcg
     2641 gacggtttcg tcggtgcggc tgcggttata ggcggcgggc aggtcgcgct cttcgtttgc
     2701 ccatgcccac tccggcggca gattatcaca cgcttttctg taggctggca atgcgctttt
     2761 cagccataat tccatctctt cccgaagcac ccaatcttcc aaaatctgtc taaatgccga
     2821 tgagaaaata tgattttgca gaaagttatt tggattgaag ccggtgtcga aggcgcagtt
     2881 atgatcaata acaatcaagg aattgttgca atttctatac aaaaggttag ggttgccgat
     2941 tgttctgtct tcattacgga caaaccaatc gaaagcggcg atttgccgct gcatgactgt
     3001 atcgattcgc gggatgtccg caggctccag taacgcacag cctttttgcg ccagtgagcc
     3061 gaaacagatg cctttgccga tttctttcat ctttgcgggc aattcttcgt acagctcttc
     3121 tccgacttcc aacagctcaa acggagcaac gggcaagccc aaagcctgcg ccatgtttcc
     3181 gcctatccat tcgtttattt ggcttgcacg ggtggcgtgc agccctttaa cgaaatattc
     3241 cagaccattc tccgccatac agatgaacgg cgaggtaaca ccttgctccg cacgatccat
     3301 tattgtctgt atctgcaaca tttcttttct ttatcctatg tttcaacaca caggacgaca
     3361 cataaagcac cgccctatgt gttgccctga ttcggaaggg gttacgcccc tcccaaacag
     3421 agtctgatcc tgccgcccta aagggcgggg tttcaaccga aaaggaaata cgatgaaatt
     3481 tacactttgt gtaaatctgt aaatccgact ttttgagatt tccccctaga ggaatttgtc
     3541 aatccagcac gctccaccaa aataccctac caataacggt caggctgtct aaagaggcgg
     3601 tttcgtcggg atagaagccg ctgttgtggc tgcgtatcag cacgctgttg ccgggctgcc
     3661 gtatcaggta tttgacgcgg aacatgccgt cttgggcgaa ggcgtatatt ttgccgtccc
     3721 gtatggcggt ttcgcccgta tctacggcga ttgccgcgtc ttctgcgatt ttttcctcca
     3781 tgctgtcgcc ggtcagggtg cagcagaaga cgttgtcggg gttgatgcct ttgcgcctta
     3841 acgttgattt gccgaacggc aggcggtagc cgttgtagtc ggggatttca tacgcgcccg
     3901 ccccgccttt gaagcagctc tctttgaggt aggggacgaa aacataatca tcgtcgggca
     3961 gcgggtcgtt gctgctccac atcatcgggc ggtggatgtc tttgacttcg tggggcaggt
     4021 cggggttgat gaggacgggc gcggttcggc tgccttcgcc tgttcgcagc catgtttctg
     4081 acacgcctaa tacttgcgcc acttcaggca gtgctttggc agaaatccca cgactctccc
     4141 aatttttcaa agtttgttgg ctgatattca aaagacgcgc cgtctccgca ggggtatttt
     4201 tcccttttgt ttttgccgca tcaaaaagtc tgatgactgt ctcgtgtttt tccatttttc
     4261 aactccttgt ctgttgtgat tattttctta tatttaaaca aaatgttgtt acacaagact
     4321 tgatttttat ctaaacatag tgtttaatat tagtattaaa tttaaacatt tagtttactt
     4381 atggaagaaa taatcaatga agacaaacgc ttactgcaat caatcggcag ttacgcagaa
     4441 attgggcgga aaacaggaaa cagcccccaa tgcgttttta attggtcgaa gcgcgggata
     4501 ccggcgcgaa taaaactgca atatcccgat ttgtttttaa acccgaagaa aacagacgac
     4561 caacccaaat aaaaaaaccc gtcggggatg acgggcgggg gcggttgttt gaaccgcttt
     4621 aaaggaggtt tgattatgaa tcaaaaacaa acgcaatgca aacaaattgt cgattacatc
     4681 cgtaaaaagg ggtgcatcac atcccttgag gcttatcaga acctgaaggt gacgcagctt
     4741 gcggcacgga taaccgactt ggaaggcagg ggcttcgtgt ttgccaagcc gaaatacaag
     4801 gtcggtaact gtaaaaatcc cgttgcccat tactcaatcg caaagtcagg aattgaacca
     4861 tgagccggga acaaagaata cgcggagccc gcttgccggt gccggctcat atgcaggcgg
     4921 aagacgcggg caaggcacgg gaagcgttgg acgggcgggt cgaaaaagtg aaaggggttg
     4981 atgatgaagc cgtctgaaag tttgagagcg gcaggcaggc caatcgcgta ttacccgaaa
     5041 ttggcaaagc ctttgggcgg tgtaaatgcg gcaatattat tcgggcattt cttctactgg
     5101 aacgataaga cgcagtatga atcaggcatt taccgaacag cggaagaaat tgaaattgaa
     5161 accgggctgt ccgttcagga acaaagaacg gcacgggcaa agctgaggga acgcggcgta
     5221 ttgattgaga ctgaaaaacg aattgaacac cgcatttact acaaactgaa tttagacgct
     5281 tttgatgatt tgatgttgca acattcgggg ggtggggaac caacagcccc gaaatgcaat
     5341 atcaacagcc ccgaacttca aaatcaacat tcggggggtg gggaatcaac agccccgaaa
     5401 tgcaatatca acagccccga acttcaaaat caacattcgg ggagtgagga atcaacagcc
     5461 gttataagaa cagaagattt aacagaagat ttagcagtat ataccccctt acccccaaac
     5521 gccggaaacg gcaaaggcgg tttgaacgct gacgcgtttg tttccgctga cgcggaaacg
     5581 tgcgggcggg aaaccggcga accgacttcg ccgaaggccg aaagcgacag taacggcaac
     5641 ggcggccttt cgggaaaacc gaaaaatgcg aatgttccgc gccgccgcaa aacccacggc
     5701 gtaccgcttc aggaaatcgc cgatttgtac aacgaagttt tgggcggccg gttgccaagc
     5761 gtccaagtgc tgaacgacac gcgcaaacgg gcgattgcaa accgctggtg cgagatgctg
     5821 ggaacggcgg cgccaaacgg caaggtgagg ttcggggaca aggaaaccgg tttggcctgg
     5881 tttgccggtt tcttccggaa agtggcgatg aacccgtttt ggatgggcga aaaccaaaca
     5941 gggtttgcgg tcggcttcga ttggattttc aaggcgggca atttcgtcaa aatccttgaa
     6001 tggcatccgc ctaaaacgaa ccaggcggca aggggaaggg catgaaccga atcgaggaaa
     6061 cggaagcggt ccaatcactg gccagcgtag gggcggaaca gaacattttg ggcggcatct
     6121 tgattgaacc gacggcgatt gcgcggtgcg caatcctgac ccctgaaaag ttttaccagg
     6181 cgcaacacag gattattttc cgcgctctgc tggatatggc ggcggcaaac gagcctatcg
     6241 acatcatcac gctgaacgac aagctggaag cgcgcggcga ggcggaaaac gcgggtggcc
     6301 tggcttactt gatagacctg aaccaaaaca ccccaagcgc gaagaatatc agccggtacg
     6361 ttgggattgt gaacgacagg tttgtcgagc gcggcttgct gaaggcttcg gcggcgattg
     6421 aaaaaatcgc ggtttccaaa gacggcggga cggtcgcaga aaagctgtct aaggcggccg
     6481 acgaattggc ggcagccggc aaagacgcgg taaagcgtga aaccaagaca ttcggccaga
     6541 ccgttgagga tttgattggc ggtttggaca aaaggcttga cggcgtgcgt ttcggattgc
     6601 ctaccggctt gatgaagctt gacgggatga ccggcggttt gccggatgga aacctgattg
     6661 tgattgcggc gcgtccgtct atgggtaaaa cggttttggc ggaaaacatt gcgcgattcg
     6721 cgctgaagca gggcaaggca gttcatttcc aaagctacga aatgagcgcg gtagagctgg
     6781 caaggcgcgg catggcggct gagtgcaata tccccatgca gaacctgaaa accggcaatc
     6841 tgacgcaaag cgattacgcc aatatgccga tttacgtcag ccaagcgaaa gagtggaagt
     6901 ttgacgtgaa ctgcgacctg ctcaacgttg acgagctttg ctttttggct aaggagaaaa
     6961 aactcactac cggcttggat ttgttggttg tcgatcacct tcacatcatg ccaagggcag
     7021 ggagggacga ggtggcagag ttggggaata tatcgcgccg tttgaaaaac ttggcggcag
     7081 agctgaatat ccccgtcgtc ttggttgccc agttgaacag gggaaacaca aagcaggcag
     7141 acaaacgccc gaacatggca gacattcgcg gcagcggcgc gattgagcaa gacgcaaaca
     7201 tcatcatcat gccgcaccgc gaaagctact acgacggaaa cgagaatccg agcattgccg
     7261 agctgattat cgccaagaac cgggacggcg aaacgggaac ggtggtttgc ggctggaaag
     7321 ggcaatttat gaagttcgag gaagagcctg atttggcatg gcaagccccc aaacatgatg
     7381 aatatgaccc ttacagtgtc tagtgcggga ggccggtaaa tgcgtgaaac ctgtttctat
     7441 tgcaaccatg ccgacttcaa aaccaacacc ggcacgccga tgcgcggttt tgcgaaatgc
     7501 gcgaaggcgc ggaatgcgga ggaaaaagcg acgtactacc cgcgaaccaa tccgtgcgcc
     7561 gccggggcgt ttcagacggc atcgggggcg gcagtcgcaa aaaggacggc ggtgcttggg
     7621 gaatatcccc ccgcaatgcg ccgaatttga gcgggaaggc gggtaaaacg ctttgggaat
     7681 atcccagcct acccgagatt taaaaa
//
LOCUS       DAXFWM010000084         7524 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000084 DAXFWM010000000
VERSION     DAXFWM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.084"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(45..251)
                     /gene="rpoZ"
                     /locus_tag="ACFI8B_001940"
     CDS             complement(45..251)
                     /gene="rpoZ"
                     /locus_tag="ACFI8B_001940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC3835082.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(309..926)
                     /gene="gmk"
                     /locus_tag="ACFI8B_001941"
     CDS             complement(309..926)
                     /gene="gmk"
                     /locus_tag="ACFI8B_001941"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC3835083.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            1054..1653
                     /locus_tag="ACFI8B_001942"
     CDS             1054..1653
                     /locus_tag="ACFI8B_001942"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC3835084.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(1699..2487)
                     /locus_tag="ACFI8B_001943"
     CDS             complement(1699..2487)
                     /locus_tag="ACFI8B_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC3835085.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            2813..4168
                     /gene="yegQ"
                     /locus_tag="ACFI8B_001944"
     CDS             2813..4168
                     /gene="yegQ"
                     /locus_tag="ACFI8B_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC3835086.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            4299..4772
                     /locus_tag="ACFI8B_001945"
     CDS             4299..4772
                     /locus_tag="ACFI8B_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC3835087.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(4981..7354)
                     /locus_tag="ACFI8B_001946"
                     /pseudo
     CDS             complement(4981..7354)
                     /locus_tag="ACFI8B_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT         1864 a         2139 c         1767 g         1754 t
ORIGIN      
        1 actgcacggc ggcagagtgt gccggcgtgc gtttccggca gaatttattt attgcgcgtc
       61 aacagttccg taccgatatg cccggctgcg atttccctta aggcggtaac ggtcggtttg
      121 ttattgcgga catcgtccac aagcggcgta ttgccatttt caagctggcg ggcccgacgg
      181 gccgctacca atgtcaggtc aaaatggttg gaaatttttc cggtacagtc ttcggtggta
      241 atacgtgcca tattatttgc tttctttcaa aaatatttaa attgggaaac cggatatttt
      301 cgcggttttt aggaattttc caacaaatct gcaataaatc ccagttgccg cgaccttttc
      361 agacggcagg cattcacaat atgaagcaaa tccccctccg ccttctccag gtcgtcattg
      421 accacgacaa agtcgaacaa tacggactgt tcgatttcat gccttgcctt cgacagcctc
      481 ctttgaataa cttcccgact gtccgtcccg cgccctttga ggcgcgcggc aagcacgtcg
      541 aaagaaggcg gcaggataaa gataccgaca gcttccggca gcgcgttgcg aacctgcgcc
      601 gcgccctgaa cgtcgatttc caaaatcacg tcatagcctg ccgccgccaa cgcattcacg
      661 ccctccgtgc ttgtgccgta atagttgccg aatacgtctg cgtattccaa aaaagcctcc
      721 tgcgcgataa gcgattcaaa ctcttctttg gaaacaaagt gataatgtac gccgtttgct
      781 tcgccttcac gcggcgggcg cgtcgtatgc gacacggaaa cgcgcaaacc gttatggttt
      841 gccaacagcc gcgacaccag cgtggttttg cccgtgccgg aagcggccga aatgataaag
      901 atgttgcctt ttcgataagc ggacatattt tttacctgta tattttccaa ccgattgtat
      961 cacaacggac accctatttc atatttgccg atgcccatat tttgccgcta ttgttttgat
     1021 tgatttggca agcggcaggc tgacggctac aatatggcgt taaaaacatc aaacttggaa
     1081 cacgcaatgc tggttcatcc cgaagctatg agtgtcggcg cgcttgccga caaaatccgc
     1141 aaaatcgaaa actggccgca aaaaggcatc ttattccacg acatcacgcc cgtcctgcaa
     1201 agtgcggaat acttccgcct tttggtcgat ttgctggttt accgctatat ggatcagaaa
     1261 atcgacatcg ttgccggctt ggacgcgcgc ggcttcatta tcggcgcggc actcgcctac
     1321 cagctcaacg tcggcttcgt ccccatccgc aaaaaaggca agctgccttt tgaaaccgta
     1381 tcgcaaagct acgcgctcga atacggggaa gctgcggtgg aaatccacac cgatgccgtc
     1441 aaacccggtt cgcgcgtcct gctggtcgat gatttggttg ccacgggcgg cacaatgctt
     1501 gccgggctgg aactgatccg caaactcggc ggggaaattg tcgaagccgc cgccattttg
     1561 gaatttaccg accttcaagg cggcaagaat atccgcgcaa gtggcgcgcc cttatttacc
     1621 ctgcttcaaa acgaaggctg catgaaaggc tgaaaaccga ccctgccgtc tgaaaccggc
     1681 agggttgtta ttatacgttc aaatcacacc caaatcttgc aagcccctca acacgccgtc
     1741 ttcatcaacg ccggggcaaa catatttcgc cgcttctttc gctgcctgtt ccccgttacc
     1801 cattgccacg ccgaacccga cttcagacag catttccaca tcgttcaaac cgtcgccgaa
     1861 cgccatcacg tctgccattt ccaaacccaa tgcttcaacc acgcttctga tgccgtctgt
     1921 tttcgacatt cccgcaggca gcagatcgac cgcttcctcg tgccagcgca ccgttttcaa
     1981 gccttcccgt tccacaatat ccgaccaaag cggcatttcg ttttcctccg caaacaccag
     2041 catctgatac accggtttgc ttgaaaaata atccttatcg gcaaaaaaat cgctggcgat
     2101 atgctgcaag gcgcggcaca cgcattccga cagcgcggac acggcgatcc cctctccgcc
     2161 gacaaacgca taatccatgc ccaagccatc caaatgcgca caaaccctgc ccatcaaacc
     2221 ggtatccatc ggtacttcgc gcacagtttt accgtgcagc agcgcaaact gtccgtttat
     2281 cgttaccacg gcatccatac ccgtttccgc catcatgtcc ctgacctttt cgggaatcgt
     2341 cgccaaagac cgccccgttg ccaacgccgt caatatacct ttgccgcgca aagccgccac
     2401 cgccgttttc acggaagggc gcaaggtatc cgtatatttg cggtacagcg tatcgtcaat
     2461 gtcgaaaaac acgattttgg gattcatcat attctctctc gcactcaaac tgccgcattg
     2521 tatcccaaga aggcaaatac ttgataaatc cttataaatt tcccggcaaa attgaccgcg
     2581 aacacgaaaa ggcggataat ccgcccatct tcaaacaccc ttcagacggc atttgcagca
     2641 atgccgtctg aaacattttt acaaagcata caaatcatgc ttcaacacac aggacgacac
     2701 ataaagcacc gccctattat gtcgtcctga ttcggaaggg gttacgcccc tcccaaacaa
     2761 agtctgatcc tgccgcccta aagggcgggg tttcaaccga aaaggaaaca cgatgaaagc
     2821 acccgaactc ttattgcccg ccggcggatt ggaaagaatg cgcgccgcct acgattacgg
     2881 cgcagatgcc gtttacgccg gcagcccgcg ttactccctg cgcgcccgca acaacgaatt
     2941 tgccaaactc gacgtcctcg agcaaggcat taaagaagcg cacgaacgca acaaaaaatt
     3001 ctttttgacc gtcaacaccc tgccgcacaa ttccaaactc aaaaccttcg ttgccgacat
     3061 ggagccgctg attgccatga aacccgacgc gctgattatg gcggatccgg gtttgattat
     3121 gaccgtgcgc gaaaaatggc cggaaatgcc gatccatctg tccgtacagg cgaacaccac
     3181 caactactgg ggcgtgaaat tttggcagaa cattggtgtc gaacgcatca tcctgtcacg
     3241 cgaattgggc atggaagaaa ttgccgaaat ccgccaagaa tgccccgaca tcgaactcga
     3301 agtcttcatc cacggcgcat tgtgcatcgc ctactcaggc cgctgcctgt tgtcgggcta
     3361 tttcaaccac cgcgatccca accaaggcac ctgcaccaac tcctgccgtt gggactacaa
     3421 agtccacaac gccacggaaa gcgaggcagg cgatgcccag cttctgcaag gtttcaactt
     3481 tgaaaaagcc caagaagaag ccaaccaaaa ctttgaaggc atcaacggtc aaaaacgcca
     3541 tccctacgcc gacaaagttt tcctgattga agaatccaac cgcccgggcg agatgatgcc
     3601 gattatggaa gacgaacacg gcacctacat catgaactcc aaagatcttc gcggtatcga
     3661 agtcgtcgaa aaactcgcca aaatcggtgt ggacagcctc aaagtcgaag gtcgtaccaa
     3721 gtctctctac tacgtcgccc gcgtcgccca gtcctaccgc aaagcgattc acgatgccgt
     3781 cgcaggccgt ccgtttgatt acagcctgtt gagcgaactc gaaggccttg ccaaccgagg
     3841 ctacaccagc ggcttcctcg aacgccacca aactcaggat tatcaaaact acctcagcgg
     3901 ccattccacc gccaaacaaa gccaatacgt cggacacgtt accgaaatcg acgaaaatgg
     3961 ctgggcgacc atcgaagtta aaaaccgctt cgccgtcggc gattcgctcg aaatcatcca
     4021 cccgagcggc aaccaaacca tcaaattgga acaaatgacc cgcaaaggcc aacctgtcga
     4081 tgttgccccg ggcaacggca ttcaggtcaa aatccccaat atgcagggca aagaaaaagc
     4141 cctcatcgca cgcgtgttga acccctgagc cacaatgccg tccgaacccc gttttcagac
     4201 ggcattttgt tgaatcttac tgtacaatgg caatgatttc ccacagcaaa caaggacatc
     4261 cgacataaaa aatcctcata ctctccgccg ccgccctgat gctggcagcc tgccaatccg
     4321 gccgcgcgct taaaaacgcg cacacggcat ccgccgggac accggccgga gaatcatgca
     4381 aagttatccg cggagacacc gcaggaggcg gcaaaaacat catttaccgt tgtagcaacg
     4441 gtcaggcggc aataaatgca gccaccgccg ccggcgtatt ggaatcaggg attcccgtca
     4501 gcttcggcgg cggcgtaccg gttgcaaacg gcaggaatct agttacacgg caagcggcaa
     4561 acggcgttgg caaatccgac tccgaggcct gcgaacacgc cctaatcaat gccgcccgca
     4621 aattccagca gaccgccggc aaactcggcg ggcgcagtgt taccggcttc cacagctact
     4681 ttggcaagca atcgctgcaa ggcggacaat acgactgtca ggcaggttcg ttccacgtcc
     4741 gcgtggtcat gcgcgccaat gtcgtccgtt aacatatcgg caatcaaaaa atgccgtctg
     4801 aaacattttt cagacggcat ttttaatcct gcaacattac tccctgccct gagttcggat
     4861 actgtatcaa tataaaaccc catcacacag atttacggta aaaagccgtc cgaatgagtt
     4921 cctgaaaaca attcggacag ctttaatccc caacgaggca attaatccaa taatactaga
     4981 ttaaaacttc cattccagcg atacggcgta attacggcct gaggcgcggt agcggtctaa
     5041 gcctttgcca tctcggtcga ccgcgttggt ggtgctgtag ctatacaaac cgcgcaggga
     5101 atcccaagtg gtgtatttgc ggttgaacac attatatacg cctgcacgca aagtcaggtt
     5161 tttagccggt ttgtagaagc cgtacatatc aaacacataa gccgacttgt tcagccacgg
     5221 gtaatctttt acctttttct gcaaaggcgt accccggccc ttgttttcat aaacggtgta
     5281 ttgcgcgtct ttggcctttt tcgcacccag ataagtcagg cgggagaaca caccccattt
     5341 ttcgctcggg cttcgtagtc gacaccggca atcactttcg gcggctgtgt ggacagcagg
     5401 ctgttgtcgc ccgacagttt gcttttcgca taacccagcg agccgaacaa tttccagccc
     5461 tcaggaacaa aagacgctat tttgtccaca ttcagacggc ctgtcagctc aagaccacgg
     5521 attcgggcct tatcgatatt ttgcatctgc cattccggtt tttcggaata aggattgcta
     5581 cacataccgt agtagtaatt catctgagta cagccgacat cgccgctggt ggtcagcttc
     5641 tgctcttcag acaagaagtt gcggtaattg ttttgataca ggttggcatc caaagtacct
     5701 ttttcgctgc ggccttgcag agacagggtg tgggtggtgc tgcgctcggc tttcaggttg
     5761 ggattgggca gccaattacc cgaaccgtgg ttgtaagtga aatacacttc ggacgcattg
     5821 gggacacggt agccggaagt aatgtcgtaa ccgacatgcc aagcctgatt cagttgcgcc
     5881 gccaaaccga caaatccgct ccagccttta taagtattgg ctgcaggcgg tgttttgtca
     5941 caagcatgac actcggcatt caattcctga ggcgtcattt tggtatgatc gtaacggata
     6001 tctgcacgtc tgctgaacac gtcgttccat tggatttgat cagacagtga gaaaccataa
     6061 ttagtggttt tcacagggtg ttgaatcgag ctggtagtac gggatatctg tccgctgaag
     6121 taataatcgt cgcggtttaa gttttcaaac tcacgccgac tggcgaaagt tttaaacgac
     6181 aagcgatgtt ggccgcccag ttgcaacggt tggctgtcca tacgcaaagt aaaacgtttg
     6241 aatcgggtgt ccatgctgcg gttgtatatt tcgtccaaat ccttctgatt tatagttgcg
     6301 cgtccaggtg gaataatcca tcgggaacga gcctttgttg ttaaccgccg ccactttggt
     6361 tttctgataa tcgaagtccg ccttcaaaga cgacaaccaa tttgaatcag gcgtccattc
     6421 gtaaaagagg ttggcattgc gccgtctgtt tacgtcatcg gcttcgcgcc aggaagaagc
     6481 ggtcaggtta taagactctt caaatcgtgt aattatgccc ctgctggccg ttaaacgatg
     6541 cgccgatgcg gtgcttgtcg ttgatttgat aagcaatctt acccaagaag ttgtggtatt
     6601 tgtgtttgga cggatcaggg ataccgcgtg ccgaaccacg gatattcgct ccgctgccag
     6661 caccctctac cggatagcca cgctcgcccg cgctttcggt ctcatgaccg cgacgttgcg
     6721 aatacagcaa agcggcatcc acgcggtcgt tgctcacacc gaaaccgagt gtatttgtcc
     6781 attcgcggtt gcggctgctg taaccgtttt tcatcatcac gccgaattgc ctgtcgtcca
     6841 acagcaaatc atggccttgc agggtttggt aattcacgcc gccacccaat gcgccgctac
     6901 cggtattgaa agagtcagcg cccttcgcga tttcgatgtt gcgcacgagt tcggggtcga
     6961 tagacaggcg cgagctgttg aagttgccat aacgtgcata cagtgagttt tcttccgaat
     7021 caggcaggct cacgccgtca atgctgacac cgacacggtt gccttccacg ccgcgcacag
     7081 caaagccttt ttgatggcgg ccgctatcgc tcaagccgac gtcggtggag taacgcacca
     7141 agtctttgtt gtcgcgtatc atttcctgtt gaatgcggcc gaggttgaca cgttccacgg
     7201 ttgcaggcgc attaagctgg tctttaacgc gcacttcttt tatctctgct ttaacgggtg
     7261 tggtttcggt tgcagcttca tccgctgcca agaccggatt gccgaaaata ctgccgacca
     7321 gcgcggcaat aggaagcatg tgtaatggtt tcatattatc aactcaagat gtaatggatt
     7381 gttcatccat cagttaattc ataataaatt ttctgataat cattaatatt taataagaca
     7441 gcaacccgta caaataaatc tgtgtcgtgt aattaaaggt ctctgcaaac atctatgccg
     7501 aggccttgtt tgtttcatta tttt
//
LOCUS       DAXFWM010000085         7267 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000085 DAXFWM010000000
VERSION     DAXFWM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.085"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..1032)
                     /locus_tag="ACFI8B_001947"
     CDS             complement(<1..1032)
                     /locus_tag="ACFI8B_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC3835088.1"
                     /translation="MSDLVRYDPLEHGRLVGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMAGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAAVAAAKGTPEFAALAKESAKG
                     GLGGAARHAAAYATESAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVGFAGMK
                     AYAAVTDPLR"
     gene            complement(1029..2225)
                     /locus_tag="ACFI8B_001948"
     CDS             complement(1029..2225)
                     /locus_tag="ACFI8B_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835089.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERDRAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPYAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQKGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAALGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(2462..2854)
                     /locus_tag="ACFI8B_001949"
     CDS             complement(2462..2854)
                     /locus_tag="ACFI8B_001949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835090.1"
                     /translation="MAKSGNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQ
                     AGGQIAAMPQIAPVADAVMQGAGYVRPARGDDPGFPVPAMPPETQIPPEGIPEAYGAD
                     TGPMTAVPPKSANHAQTGMETPTVSDNL"
     gene            complement(2870..4729)
                     /locus_tag="ACFI8B_001950"
     CDS             complement(2870..4729)
                     /locus_tag="ACFI8B_001950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835091.1"
                     /translation="MEALLMGTDVPETGVLPDKNGEPLTIGEYRLFVDEMMNQPAWRA
                     VADKEMDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTAD
                     GETGGRDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYE
                     CGVIHRNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDW
                     RISGEMLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRD
                     KKTGRTVEFDGANPNHREMAANGALLFAASVPRMRRAFVVGDLVVRDEPTPYPHQKFP
                     YVPFFGFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVRTKGIVDMSDEQ
                     FRRNIARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRATIRQISGITPS
                     FMGNRGNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMIIEDLGSDEQTV
                     VIEGDAVTQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPSTNSYRSQQLGA
                     MSEAVKSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKEVRVQETPEQIEARIA"
     gene            complement(4714..5988)
                     /locus_tag="ACFI8B_001951"
     CDS             complement(4714..5988)
                     /locus_tag="ACFI8B_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFC3835092.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCAREIQKSMRDSVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMNKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     AAGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            complement(5969..6211)
                     /locus_tag="ACFI8B_001952"
     CDS             complement(5969..6211)
                     /locus_tag="ACFI8B_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835093.1"
                     /translation="MAENLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIE
                     RKSYGMDTAESKISESAKTAGIRIEFVGPEDDGKDG"
     gene            complement(6266..6508)
                     /locus_tag="ACFI8B_001953"
     CDS             complement(6266..6508)
                     /locus_tag="ACFI8B_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835094.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYADFNLK"
     gene            complement(6508..6930)
                     /locus_tag="ACFI8B_001954"
     CDS             complement(6508..6930)
                     /locus_tag="ACFI8B_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835095.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(6995..>7267)
                     /locus_tag="ACFI8B_001955"
     CDS             complement(6995..>7267)
                     /locus_tag="ACFI8B_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3835096.1"
                     /translation="ASSIDHINRDKTDNRISNLRDVTHAENMKNRGKFKNNTSGHTGV
                     YFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKAREAASKDFGFVV"
BASE COUNT         1443 a         2488 c         1689 g         1647 t
ORIGIN      
        1 cctgagcggg tcagttaccg ccgcataagc cttcattccg gcaaacccca ccgcagaatg
       61 ccccaaagaa gagaaagcct ccaaagccgc ccccttctcg tcccattccc cgtttgccag
      121 ccccgtaccc aaatactcgc ccgcaaactc accggccgat tccgtcgcat aagccgccgc
      181 gtgccgtgcc gcaccgccca aaccgccctt ggcagactcc ttcgccagcg ccgcaaattc
      241 aggcgttccc ttagccgccg caaccgccgc cgcaacaccc attttctcca acgcggcttt
      301 cccggcggcc tttttgccca tattcaaaat actgccgcca agtttcatcg ccgccatatc
      361 cgccgcgccg accaccgcgc ccttgaccgc cgcatttttc aacgcacccg gcgcaccgcg
      421 gccgataaac gccatcaccg catccttgtc cgcagggtcg acgcccgccg cctctgccgc
      481 cctgtccagc tgcccgccgt attccatcag cgtattgccc aaggtcgcgc ccgcataggc
      541 gccgacagcc gcgttaccgc ccgccaaagc gcccaatatg ccgccggcgg ttgtcgtagc
      601 aataggaaca agcgcattgg gcgcctgctc cagcaccgca cccgccatct cgcccacatc
      661 ttccaaggcg ccgtccaaac ccttctcgcg ccagtccttc ttaagctccc cccacacatt
      721 cgacaggccg ccgccgacac ccccgccctt ttcccaagcc tcgcccagct ccctgcgcgc
      781 ttgggatttg cgcccctcct gaatcttccg ataatccata tcctcggcct tgagccggcc
      841 cagttcctct ttgtcgcccg taagggcatt ccaccccata cgcgccgaac gcatagaatt
      901 tttaaacccg cgcgtcaacg ccgtatcgtc ggcggccgcc cgcgcatcct tttgcgtaaa
      961 gccgcggtac tccttcaaac ccccgacaag ccgcccgtgt tccaacggat cgtatctgac
     1021 caaatcactc attgctcaac cctcttctca cccttctcat tctcataaat gattttaccg
     1081 ccggccgttt tctgaccgtt cggcttaaag cccaacgccg cataacgctt ctcgcgctct
     1141 cccgcaaact ccacatccaa aggcttgccc gtccgcaaat cgacaatccc ctcgcgcttc
     1201 gccgtcaagc ccgtatccgg gtcggcaacc tcataactga ttgtcttgaa cacattcggg
     1261 tcaaagccct tctccccttg ccgctcaaac ttcccgttaa gcgcatcaat ctgccgcgca
     1321 atctccccgc gctgcccgtc cgacttcgca aaaccataag cccgcgtcaa atcggcgatg
     1381 cgcttcctat cctcaagctc cagcgcatcc gcctcacgct tcatctcaaa cccctgccgc
     1441 ttcatcccca gctcggcctg ctgcacatca aacgccctgt ccgcattcct ctgcgccatc
     1501 tcgcggttca acgcaccctc gttcgcctta accgccgcat caaaccgccc cttctgcaac
     1561 accgcctcac ggttcgccgc attctccgcc gcagccgccg ccagcctttc cctgcccaat
     1621 tccctgaccg ccgcattttt attatgttcc gccacaatcc cgcgcgccgc attaagctgc
     1681 gccgccgtca actgaccgtt caaagccccc gcataaggcg tagtcgccct cctgtaaagc
     1741 tcccgctcct caggcgacag cgcatccggc ccggcagaca ccaaaggacg ctgcgcgtca
     1801 aaatcacgca ccggtttgcc gtcccgatac acattcccgt caaatcccaa aggcggcggc
     1861 gtccgtccgc ccgcatccgt cgggcgcgta tcgcgccgcg cgaaatccgc cccggcccgc
     1921 gcatcggggg aaagcgcctt atcgccgccc gaaaacccca aaccggcatc cgcaccccgt
     1981 tcgaaagcac ccgcccggcg caaccgccgc gccccatcct caatattgtt acggctcaaa
     2041 tccatcacat tccgataacg cgccggcccg gcctcataat tcgcaacggt acgccgcacc
     2101 gcatcggcac ggtgcgcctg tgccgccgcc cgatcccgct cctccgcccc cttccgggcc
     2161 ttctcttttt tctccttttc cttcatctcc caaaaatccc cggcagacgg ctcaaacaaa
     2221 cccatcgcaa accctccgaa caaataaagc ccaaaataag caaaaaccca aacccaatca
     2281 aacgcctaaa gttaagaaca aacaaaaatc gcaaccacaa accaattaat aaacaacaag
     2341 ttacacaaaa aaacacaccg acgggccatt tccgggggca aataaaaacc gccttcgtgc
     2401 aaggcggttg caaaaaagcc tttcatttaa cgaaaggctt tttgccgccc gcgtaccgcg
     2461 ttcagaggtt gtccgacacc gtcggcgttt ccatgcctgt ttgggcgtga ttcgcactct
     2521 ttggcggcac ggccgtcatc gggccggtat ccgcgccgta ggcttcaggg atgccttcgg
     2581 gcggtatttg cgtttcaggc ggcatggcgg ggacggggaa gccgggatcg tcgccccgcg
     2641 ccggcctgac atatcccgcg ccctgcatga ccgcgtcggc aacgggggcg atttgcggca
     2701 tggcggctat ctgcccgccc gcctgcatgg ccgcataagc cgcctgcacg ccgatttgta
     2761 ccgcccgcgc ttcgatttcc ttgatttcgc tcgcggtacg ttgttccttg agcgccaatt
     2821 cccgccgttt gatgtcgttg ccggattttg ccaatgcgtc ctgcaccgcc tacgcgatac
     2881 gcgcctcgat ttgttcgggc gtttcctgca ctcggacttc tttgattttt tcaatcactt
     2941 tgtctttaaa cgggatgtcc atcagggaca ccataaacgg cagcaccgcc gcctgatatt
     3001 cgggcggcag ggatttgacc gcctcgctca tcgcacccag ctgctggctg cggtaggagt
     3061 tggtgctggg cacgtcttcc aaagccactt tcagccgtat gttttgcagg tcgttggaca
     3121 aataagcctt gccggttacg gggtcggttt caggcctgtt gatgacgacc gtccgccctt
     3181 gcgtgacggc gtccccttct atgacgacgg tttgctcgtc cgagcccaaa tcctcgataa
     3241 tcatcgcaag cagcaactcg ccgaccaatg agcggctctg gcggaagttg tccataacca
     3301 gccccagcga ctggttggac tgctcgactt ggatgctttc ctgcctgccg ctggtggcgt
     3361 tgccccggtt gcccataaat gacggggtaa tcccgctgat ttgccgtatt gtcgcgcggc
     3421 tgtcttgaag catctgccaa tgctgtgccg acaattcgaa atcgcggctg acgtcgaaac
     3481 gcgcgcccgg ctgggcggcc tctattttgt tcagcacgat gtccgcgtcc acccgtgcga
     3541 tattgcggcg gaactgttcg tccgacatat cgactatgcc tttggtgcgt accgtgcgta
     3601 ttgccgacaa accccatcgt aatttgctgt tggtgctgtt gaggttgtcc tgcgcgtatt
     3661 tcatattgcg gacatatccg taggggatgc cggtgttgtc ctcgcggaat ccgaaaaacg
     3721 ggacgtaagg gaacttttga tgcggatacg gggtcggctc gtcgcggacg accaaatccc
     3781 ccacgacaaa ggcgcggcgc atacgcggga cggaagcggc aaacaatagc gcgccgtttg
     3841 ccgccatctc ccgatggttt ggatttgcgc cgtcaaactc caccgtgcgc cctgtttttt
     3901 tgtcgcgcag gcagtctgcc gttacccacc gcctgtacca tacttccgcc accgccagtt
     3961 cgcgcgtggt ttcattgaac caaaactctt cgctgacggt gttgcgcccc gaaatacccc
     4021 aagcgtccgc cagtccggta ttgccgccgc cgtcaagcat ttccccgctg atgcgccaat
     4081 ccgaaccgcc gcgccccatc gctttgaaat gtccggcata ttcagggaag aattgcgcca
     4141 ggcgttcagg cagcagccag cggcggcgta tcagccagcg ggcatcggac aggtcgtatt
     4201 tgtaagactt catatcccaa tggatggcgt tgcggtggat gacgccgcac tcataaggaa
     4261 actcgaaagg gttggggttg cgtgtaacct ccacccagcc gatgccgcag gctatctgcc
     4321 ccctgaacgc gtccgaacaa gccttgtcgg cacggctttg ccgttccgcg cggttgagtt
     4381 tgaagttcaa tgcgtccgcc acgtcccgcc cgccggtttc gccgtccgcc gtcacgcgcc
     4441 agtccgtccg tatcgtcgcc tcatatccct gtaccgacag cagggtcggg gtaatgaggt
     4501 tttcaacggc gggcggcagg cccaactcgc gctgtttctg taaaagctcg ttgtcaagct
     4561 gcctgccgtc ggcgtagtcc atttccttgt cggcaacggc acgccatgca ggttggttca
     4621 tcatttcatc gacaaacagc cggtattccc cgatagtcag cggttcgccg tttttatcgg
     4681 gcaatacgcc tgtttccggt acgtccgtcc ccatcaataa agcctccaat ccgatacggg
     4741 cgtttccgtg tatcccgtat tgctgcctgt atccattaaa tcaaccgcct gcgccaggta
     4801 gcggaacata tccgcgccgt gcgaatattc gtcgtgcagc ggccccattg ccacgcctgt
     4861 tttcgcatgt atttggcggc cgtaccgttt caggcattcc aaaagccgcg ctgttttgtc
     4921 tttatcgaag tacactttgg gaaacagcat ccgcgccgcc ctgatgcctt cttcgatacc
     4981 ggcggcgttt tgcacgaaaa ccgacttgcg ccccagtccg gtcaaaatct ccatcgtact
     5041 tttgcctgtt tggaagttgc gcgtcctgcc gtcgtgcggc aggaagtccg tcccccaccg
     5101 gtagggcagc ttttccaatt cggcaacata ccagtccaac gtccggtgcg tgtcttcgat
     5161 gtagcctatg atgcgcacgc tcgtcaaatc gcgctgcacc agcccgatgg tcatggcatc
     5221 gttccagccc aaatcccaaa ccgtatgcac gggcaaagaa gaatcataag ggacgagcgt
     5281 aacgcgtccg gaatgaaaag cgtcctgtat ttcatgccgg taaaccgccc cctccgatac
     5341 catgcgcggc ctgccttccc aaatattccc gtagtcctct ttattcatcg aacgctgtgc
     5401 tttgagccgc tcccggttca atgcttcggg aaaccacgga ttgtcgcgcc agttgatttc
     5461 gcaaagccaa gtgtcttcgg acggcatagc gataaaacgc cggtaggttt cgtccgtctc
     5521 catatcggga ttgagggtaa tccaaatttc cgaaccttct ttgcgtatgg tcggcgtgag
     5581 cacgtcccag ctttttttac tgacgccgtg cccttcctca acccatacga tgtcgatacc
     5641 ttcaaacgat ttgatgctgt ccacggtatg cgactgcagg cccgaaaata caaacagcgt
     5701 gccgtttgcg cctcgtatct cgaagtcggt tatttcgtag aaatgcccca aacccaactg
     5761 cgccactttg tctttcaaca ggcggtgtac ggaatcgcgc atcgattttt gaatttcgcg
     5821 cgcgcataaa atacgcaaag ggcgttgcgc ccccagcgcc agcagtgcgg atgccgcgcc
     5881 gtgcgatttg ccgccgccgc gcccgccgta cataaccttg taccggcaag gcttgaacag
     5941 cccgtccagt tttgcgggca atttcaaatc aaccgtcttt cccgtcatct tcaggcccta
     6001 caaattcgat acggatgccc gccgtcttgg cggattccga aatcttcgat tcggcggtat
     6061 ccataccgta tgatttacgc tcgatttcca ccaaaacctt aaaccccgcc gccaccgttt
     6121 ggaacatacg cgcctttgtg aatgtgtcct ccgggcttcc gtacaattcc gcgacaatct
     6181 gctccgcgct tttggacagg ttttccgcca aattccgatg cctgcgctgt acggcggcaa
     6241 cccgtttcgc ttccgcgtcc gttacttatt taaggttgaa atccgcataa tccggcgcat
     6301 tgccgttttt gtccgcggac agcaccatcg cccgcgcctt tgcctgtacc tcgtcattca
     6361 aatcgcgcgt ccacacgcgt tctttggcaa ccctgccgac gtgttgcgcc gaaacattgt
     6421 atttgcgccc gatttcggca atgctcaaaa ccccgcgccg gtaatccagc tcgactttgc
     6481 gccaatcttc atctgacaat ctcggcatta aaggtccaac ctcccgtttt tggcggaaca
     6541 ttccagataa ttccgcaata ctgaaaactc gcgccccatc tgaatactgg aaaactgctg
     6601 cccgaacccc gacatcaaag cccccaacga gcgcatttcc tgcgggttga ggtgcaatac
     6661 cgtatcgcct atttccaata cgcccatccc cgaatccaat accgatacgc tgacggggcg
     6721 cgtctcgcca aaccctatgg cgggcttgaa aatccccctt gtcacgcggt agattttgcc
     6781 gtgctcgacc agcagcttgg taaatttagt gatttccgac attttcaggc ctgtcgtttc
     6841 cgccaaagcc tcgcggctga ccacctgctc cgcgttgtgc aaatccacaa ccccctggta
     6901 caccttgtca atatccgaca atcccgacat caaatcaaac tcctaaacat cccaaacgat
     6961 tccaaactcc gccgccgccc gcgattgcag gcggttacac tacaaagcca aaatctttag
     7021 atgctgcctc tctcgctttc actgcatctt caatatgttc aaataaacct aatattttat
     7081 tttttctatt aaccataatc ctagcttgcc atttcttaga cggcttatgg aaataaaccc
     7141 cagtatgccc gctagtatta tttttaaact tccctctatt tttcatattt tcagcatgtg
     7201 taacatctct caaattagat attctattat ctgtcttatc cctattgata tggtctattg
     7261 aagaagc
//
LOCUS       DAXFWM010000086         7222 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000086 DAXFWM010000000
VERSION     DAXFWM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.086"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..119
                     /locus_tag="ACFI8B_001956"
     CDS             <1..119
                     /locus_tag="ACFI8B_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3835097.1"
                     /translation="YNGIDRKPFPPLLRECEFRLNFGTPPRQLKILRDRCGI"
     gene            complement(1097..1780)
                     /locus_tag="ACFI8B_001957"
     CDS             complement(1097..1780)
                     /locus_tag="ACFI8B_001957"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC3835098.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1929..4232
                     /gene="parC"
                     /locus_tag="ACFI8B_001958"
     CDS             1929..4232
                     /gene="parC"
                     /locus_tag="ACFI8B_001958"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC3835099.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRIVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAVFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNIFLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4252..5970
                     /locus_tag="ACFI8B_001959"
     CDS             4252..5970
                     /locus_tag="ACFI8B_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFC3835100.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWMIFFSTLNPQWQWQALRIPSFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILDAK
                     TVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSHTFEHHIDTPELTA
                     RIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     DGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEALVE
                     SGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQMLL
                     NYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPSET
                     AAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRYRM
                     ERLNID"
     gene            6070..6822
                     /locus_tag="ACFI8B_001960"
     CDS             6070..6822
                     /locus_tag="ACFI8B_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3835101.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
     gene            complement(7000..>7222)
                     /locus_tag="ACFI8B_001961"
     CDS             complement(7000..>7222)
                     /locus_tag="ACFI8B_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835102.1"
                     /translation="QKRESRSLGSGNLQKSSEKSEVLDSRFHGNDGISDGILPPSHSR
                     AGGNLVRSVSVIYDKFLLPFISGFPLSRE"
BASE COUNT         1743 a         2131 c         1773 g         1575 t
ORIGIN      
        1 aatacaacgg aatcgatcgt aaaccttttc cgccgttgtt gagggaatgc gaatttcgac
       61 ttaacttcgg cacaccgccc cggcagctaa aaatcctgcg ggatcggtgt ggaatttagg
      121 gataatctag tacagcccct actattttag tagttttacc attagaaaaa ggatatgaat
      181 ggaattgcct tgaggtagaa gtactatccg ctgctcaatc tctagcaaat atctattgca
      241 acaaggttcg cctcatagat ggtcatgttt gggaggaata aatgaaaaag cgtagttggt
      301 aatttttagt tgcgcagggt gggcttcagc ccaccgtttt ttcttcattt tgggttgttg
      361 attggtgggc taaagcccac cctacgatta ctaaaatccc gtcattcccg cgtagagcgc
      421 atcataataa ggcgggaatc cagacctttc ggtgcgaaaa cttatcggat aaaacggttt
      481 cccctgcatc ctggattccc actttcgtgg gaatgacggg atataggttt ccctacggac
      541 gtgttcagat tcctgccttc gcgggaataa cggcggagca gtttctgctt ttccaataaa
      601 tgaccgtaac ttaaaatccc gtcatccccg cgaaagcaaa aatcctgcct gtcggatttc
      661 ggttttttcg ggaaactgat gaatcgtcat tcccgcgcag gcgggaatcc agaacgcggg
      721 acggcggcaa tattcaaagg ttgtctgaaa attcagaggt tccagattcc cgccttcgcg
      781 ggaatgacag cggagcagtt tctgcttttc cgataaatga ccgcaaccta aaacccatcc
      841 ttcacacgaa aacagaaaaa caaaaaccta aaatcccgtc attcccgcga taacagttgc
      901 gtaattgcgt agggtgggct tcagcccacc gtttcttcat ttttgcttgt caattagtgg
      961 actgaagccc accctgcggt tactgtttct tctttttcga ttattaattg gcaggctgaa
     1021 gccctcccta cgtttaccgt caaaaatgcc gtctgaaatg ctcaagccct cagacggcat
     1081 tttttatttc gcacccttac aggtaaaatt tctcgatgac tttcaggttg tcgtctaatt
     1141 tgtacaccag cggctggccg gtcgggattt ccaagcccat gatgtcttcg tcggaaatgc
     1201 cctcgatgtg ttttgccagc gcgcgcaggg agttgccgtg cgccgctacc aagacgcgtt
     1261 tgccgctcaa aatcgcgggg gcgatttggt cttcccaaaa cggtaatacg cgctccagcg
     1321 ttactttcag gttttcgccg tcgggtacga catcggcagg cagatgggca tagcgacggt
     1381 ctttgtgtgc ggaaaacgcg tcgtctttgt ccaaaagcgg cggcagggtg tcgtagctgc
     1441 gccgccagat gcggacttgc tcgtcgccgt atttttcggc ggtttgtttt ttgtccaaac
     1501 cttgcagtcg gccgtagtgg cgttcgttca gcctccatgt tttgatttgc ggtacgaaca
     1561 gttggtcgga ttcttccaaa acgatgttgc aggtcttaat cgcgcgggtc aggacggatg
     1621 tgaaggcgat gtcgaactca tagccgtttt ctttcagttt tttaccggcg gcggcggcct
     1681 cggcaagccc ctgctcgctc agcttcacgt cgcgccagcc tgtaaacagg tttttcgcgt
     1741 tccattcgct ttgtccgtgg cggataaata ccaattccat atcgtctcca atgtgtgaaa
     1801 gtgggaaagc tttatttata acatattttc acatttcccg tatttgattc agattcaggc
     1861 acggcgccca ctatggtttg ccattttgat ttacaataat gtcctttgct ttacattccg
     1921 catacacaat gaatacgcaa ccgcacgctt cccataccga ttccaacacg ctgatgctcg
     1981 gccgatacgc cgaacgcgcc tatctcgaat acgccatgag cgtggtcaaa ggccgcgcgc
     2041 tgcctgaagt ttcagacggc caaaagcccg tgcagcggcg cattttgttt gccatgcgcg
     2101 atatgggttt gacggcgggg gcgaagccgg tgaaatcggc gcgcgtggtc ggcgagattt
     2161 tgggtaaata ccatccgcac ggcgacagtt ccgcctatga ggcgatggtg cgcatggctc
     2221 aggattttac cttgcgctat cccttaatcg acggcatcgg caacttcggt tcgcgcgacg
     2281 gcgacggggc ggcggcgatg cgttacaccg aagcgcggct cacgccgatt gcggaattgc
     2341 tgttgtccga aatcaatcag gggacggtgg attttatgcc gaactacgac ggcgcgtttg
     2401 acgagccgct gcaccttccc gcccgcttgc ctatggtgtt gctcaacggc gcgtcgggca
     2461 tcgcggtggg tatggcgacc gagattccgt cgcacaattt gaacgaagtc acgcaggcgg
     2521 cgattgcact gttgaagaaa ccgacgctgg aaaccgccga cctgatgcaa tatattcctg
     2581 ctcccgattt tgccggcggc ggtcaaatca tcacgccggc ggacgaattg cgccgtattt
     2641 acgaaaccgg caagggcagc gtgcgcgtgc gtgcgcgtta tgaaatcgag aaattggcgc
     2701 gcggacagtg gcgcgtcatc gtaaccgaac tgccgccgaa cgccaattcc gccaaaatcc
     2761 ttgccgaaat cgaagagcaa accaacccga aaccgaaagc gggcaagaag cagctcaacc
     2821 aagaccggct taataccaaa aagctgatgc tggatttaat cgaccgcgtg cgcgacgagt
     2881 ccgacggcga acatcccgtg cgccttgtat ttgaaccgaa atccagccgc atcgataccg
     2941 ataccttcat caacacgctg atggcgcaaa cttcgctgga aggcaatgtg tccatgaact
     3001 tggtgatgat gggtttggac aaccgccccg cgcagaaaaa cctgaaaacg attttgcagg
     3061 aatggctgga tttccgcatc gttaccgtaa cacgccgtct gaaattccgt ttaaaccaag
     3121 tggaaaaacg gctgcacatc ctcgaaggcc gtctgaaagt ctttctgcac atcgacgaag
     3181 tgattaaagt catccgcgaa tcggacgacc cgaaagccga tttgatggcg gtgttcgggc
     3241 tgaccgaaat ccaggccgaa gacattttgg aaatccgcct gcgtcagttg gcgcgtttgg
     3301 aaggtttcaa actcgaaaaa gaattgaacg aattgcgcga agaacaaggc cgtctgaata
     3361 tctttttggg cgacgaaaac gaaaaacgca agctgattat caaagagatg caggcggaca
     3421 tgaagcagtt cggcgacgcg cgccgcacgc tggtggaaga ggccggacgc gccgtgctga
     3481 cacaaaccgc cgccgacgaa cccatcacgc tgattttgtc ggaaaaaggc tggatacgca
     3541 gccgtgccgg acataatctc gatttgagcc aaaccgcgtt caaagaaggc gaccgcctca
     3601 aacaaaccct tgaaggccgc actgttttac ccgtcgtcat cctcgattca tcgggcagaa
     3661 cctactcgat cgatgccgcc gaaatccccg gcggacgcgg cgacggcgta ccggtttcct
     3721 ccttaatcga gttgcaaaac ggcgcgaaac ccgtcgcgat gttgacagga ttgccggaac
     3781 aacattattt attatcaagc agcggcggct acggctttat cgccaagctg ggcgatatgg
     3841 tcggacgcgt gaaagcgggc aaagtggtga tgaccgcaga cagcggcgaa accgtcctgc
     3901 cgccggttgc cgtctatgcc tcctcgttca tcaaccccga ctgcaaaatc attgcagcca
     3961 ccagtcaaaa ccgcgccctc gccttcccca tcggcgaatt gaaaattatg gcgaaaggca
     4021 aaggactgca aatcatcgga ttaaacgccg gcgaatcgat gacgcatacc gccgtttctt
     4081 ccgagccgga aatcctgatt gaaagcgaag gcaggcgcgg cgcggcgcac aaagaccgcc
     4141 tccccgtcgc cctgattgag gcaaaacgcg gcaaaaaagg cagactgttg cccatatcgg
     4201 gcagcctgaa acagctttct tcccccaaat aaacccggtt ccgcacatat tatggtgatt
     4261 tccaaccccc gcgaacttga aaaactcaaa gaccggattc ccaatctgat caacatcatc
     4321 cgcgtcgcca tcgttttccc gctgatgatt atgcacatcc tcgggctgga aaccggcagc
     4381 cgtgcgaacc tgcacgcctc atggacggcg tgggcgtttt acttgtggct tgccattgcc
     4441 tgctggatga ttttcttttc caccctcaac ccgcaatggc aatggcaggc tttgagaata
     4501 ccgagtttca gcgccgtggc cgacatcacg atgatcggcg tgctgaccta cctgttcggc
     4561 ggcatcgatt cggggttcgg catcctgatc ctgcccttcg tgggcagctc ctgcctactc
     4621 agttacgggc gttatcccct gctctatgcc agctatgcat ccatcctgct gatattcaac
     4681 gcccttgcag acagcaatat caatatgtat ccgctgattc tggatgcaaa aaccgtcacc
     4741 aataccttcg tcgtcgtagc cggctcctat ttcgtcgcta tgattgcctc gctgtcggtc
     4801 agatacatcg accgtgccgg caaacttgcc catgaaaacc acgtcgccta ccgccgtatc
     4861 aggggcttga accaaatcgt gctcaaccgc gttcaggaag cggtcgtcgt catcaacgtc
     4921 gagcatcaga ccatactgtt caataaaaag gcaaaagatc tgctccccat gcttgaaatc
     4981 ggacagcata cctccctgtt cgaccctgtc gccatcttat gggataaaac atcctcacac
     5041 accttcgaac accatatcga cacgcccgaa ctgaccgccc gcatccgcgc cgtgccgatg
     5101 aacaaaaagc agaacaagct gctcatcctc tatatccgcc cgcaaagcga aattcaggcg
     5161 gaagccctgt ccgtcaaact tgccgcgctc ggacaactga ccgccaacct tgcccacgaa
     5221 atccgcaacc cgatgtccgc catccgccac gccgacggcg gcactttatt tttggacgaa
     5281 gtggccgacc tgcccctttc catgcaggtc aaactcttgc gcgcgattca agaaaaagcc
     5341 gtgcgccgta tcggcgacgc gaccgagcag cccgtcgatg tccgcatcgt ctgcgccacc
     5401 cacaaaaacc tcgaagccct tgtcgaaagc ggcgcattcc gccaagacct gtattaccgt
     5461 ctcaatgtcg tcagcctcaa tatgccgtcc ctgcgtgaaa tgcgcgaaaa tttgaagctg
     5521 cccaccccct acctcctgta caaacacagc cacaacaacc ggccctacac actctccccc
     5581 gccgcgcaac agatgctcct gaattacagt tatccgggca atttccgcga actcgaaaac
     5641 atcctcgaac gcgccgtcgc cctgtgcgtc ggatatacag tgcaaatcga cgacctgcaa
     5701 atccaagatg tgcaccacaa acccgtccgg acggaaaccg ccgtccccgt tgccgatacc
     5761 ttgccgtctg aaacagccgc cgcaccgtcc cgccttctcc cgttcgaccc cgataccatg
     5821 cagatacagg actatctcga caaaatcgaa cgcgacatca tcggacaagt cctcaaacaa
     5881 accgaaggca accgcacgca agccgccaaa cgcttgggca tcagcttccg ctctatgcgc
     5941 taccgtatgg aacgcctcaa catagactga caaacggcat ccgcaccatc tccgcccacc
     6001 cgaaaaaatg ccgtctgaaa cggcacggga aagcgggttc gccccacgac cgaacggaca
     6061 caatacacca tgaccgacat cctcattgac gataccgcca ccgaagccgt ccgcaccctg
     6121 atacgggcat tccccctcgt gcccgtttcc caaccgcccg aacaaggcag ttacctcctt
     6181 gctgaacacg ataccgtcag cctcaggctt gtcggggaaa aaagcaacgt catcgtcgat
     6241 tttacctccg gcgcggcaca ataccggcgc acaaaaggcg gaggagaact catcgccaaa
     6301 gccgtcaacc acaccgcgca ccccaccgta tgggatgcaa ccgcaggatt ggggcgcgac
     6361 agcttcgtcc tcgcctcgct cggactgacc gttaccgcct tcgagcaaca tcccgccgtc
     6421 gcctgcctgc tttcagacgg catccgccgc gccctcctca atcccgaaac gcaagacacc
     6481 gccgcgcgca tcaacctcca tttcggcaat gccgccgaac aaatgcctgc ccttgttaaa
     6541 acacaaggca aacccgacat cgtttatctc gacccgatgt atcccgaacg ccgcaaaagt
     6601 gccgccgtca aaaaagaaat ggcttatttc caccggcttg tcggcgaagc gcaagatgag
     6661 gtcatcctcc ttcataccgc acgccaaacg gcaaaaaaac gcgtcgtcgt caaacgcccg
     6721 cgcctcggcg aacaccttgc cggacaagcc cccgcctacc aatacaccgg caaaagcacc
     6781 cgcttcgacg tttacctgcc ctacggagcg gacaagggat aacgcccata aaacaagaca
     6841 ccgaaaaatt tgccgttctt atgcaaacga gaaaccggtt tttgcgtttc gactgttttg
     6901 gataagtcat cacaccttaa agtttgtcat tcccacggaa gtgggaatcc gattcgttca
     6961 gttttagttg ttttcaagtt tcaggcaact tccaaaccgt cattcccgcg aaagtgggaa
     7021 tccggaaatg aaaggcaaca ggaatttatc gtaaatgact gaaaccgaac ggactagatt
     7081 cccgcctgcg cgggaatgac tgggcgggag gatgccgtct gaaattccgt cattcccgtg
     7141 aaaacgggaa tctagaactt ctgatttttc agacgacttt tgaagattgc cgctacccaa
     7201 tgatctggat tcccgctttt gc
//
LOCUS       DAXFWM010000087         6951 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000087 DAXFWM010000000
VERSION     DAXFWM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.087"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            357..803
                     /gene="yciA"
                     /locus_tag="ACFI8B_001962"
     CDS             357..803
                     /gene="yciA"
                     /locus_tag="ACFI8B_001962"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC3835103.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLATP"
     gene            complement(854..1675)
                     /locus_tag="ACFI8B_001963"
     CDS             complement(854..1675)
                     /locus_tag="ACFI8B_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3835104.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1794..2252
                     /locus_tag="ACFI8B_001964"
     CDS             1794..2252
                     /locus_tag="ACFI8B_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC3835105.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2395..3531)
                     /gene="lst"
                     /locus_tag="ACFI8B_001965"
     CDS             complement(2395..3531)
                     /gene="lst"
                     /locus_tag="ACFI8B_001965"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC3835106.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            complement(3522..3782)
                     /locus_tag="ACFI8B_001966"
     CDS             complement(3522..3782)
                     /locus_tag="ACFI8B_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835107.1"
                     /translation="MAALFALCSILACLNAAAGKEGGNGFPAFGGGVGGVVPDVRRHI
                     FGKINFNIRQLSEYLLKFRIFRTGTLGAYVQFVGMEFLGIWG"
     gene            3861..6086
                     /locus_tag="ACFI8B_001967"
     CDS             3861..6086
                     /locus_tag="ACFI8B_001967"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC3835108.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEITAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6383..6686
                     /locus_tag="ACFI8B_001968"
                     /pseudo
     CDS             6383..6686
                     /locus_tag="ACFI8B_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1844 a         2056 c         1560 g         1491 t
ORIGIN      
        1 aattcccgcc attttccatc attcccgaca acaccgcaat ctcgaaaccc gtcattcccg
       61 cgcaggcggg aatctaggat gcggaatcta gagaaacctt ttaaccgata agtttccgtg
      121 cggacaggtc tggattcccg cctgcgcggg aatgacggtt cggttgctta gggtaggatt
      181 tggcgggatt ggtgggctga agcccgccct acgcatccgc cctacaatac tccttgcgaa
      241 tctgtcccgc gcccgacctt gtatgtctca aatgggttaa actacgcact tcccgacttc
      301 ccgtttcggg caggggtgca ggtccgattt atatttcaat aaaacaagga aactttatgc
      361 agcacgaaga aggcaaccgc caacgccctc aaggcgaact gctcctgcgt accgtcgcta
      421 tgccgcgcga taccaatccc aaccaagaca ttttcggcgg ctggattatg tcgcaaatgg
      481 atttgggcgg cggcatattg gcggcggaaa tcgcgcgggg gcgcatcgtt accgtcgccg
      541 ttcaggaaat gaacttcatc cgcccggtca aggtcggcaa cgtcgtctgc tgctacgggc
      601 attgcgtccg cgtgggcaat acttccctcc agcttaaagt cgaagtctgg gtgaaaactt
      661 tgatgaacga ttgcgttacc gaagaccgcc atctcgtaac cgaggcggtg ttcacttatg
      721 tcgccatcga tgcggaaggc aatccgcgcc cgattccgaa agaaggcaac cccaaattgt
      781 caggcttatt ggccaccccg taagaatacc cgtaaaaatg ccgtctgaaa ctgtcttcag
      841 acggcattcc gtatcatttg aaccaagacc tgatgcggga aaacagcgac atactctgtt
      901 tttcaaagtc ttcccgcact tcgggcacgg ctgtttttat gaccttcatt cctgcaaaag
      961 tctgcgccac aggcataatt tcaatcgtgt tcacattcat atgcgccggc cggcggtaca
     1021 gccacaatgc ggtttccgca atatcttcgg ggcggataaa ttccacaccc tcatacacgc
     1081 ccgccaccct ctcgttatcg cctttgaagc gcacgttgga aaactccgta ttgccgcaca
     1141 aacccggctc gatattggta acgcggatgt tcttatccgc cagctctgcg cgcaaattca
     1201 ggctaaactg gcgcacaaac gccttggtcg ccccgtaaac gttgctgccg gcataagcat
     1261 aattgcctgc aatcgaaccc aaattcatca cataaccgcc gccgcgttcc accatttgcg
     1321 gcaaaatctt gcgcgtcagg aacgtcaaac ccaaaacatt ggtttgaatc attgtttccc
     1381 aatcttcaaa atccgccttg tccgccgtgt ccaaacccaa agccagcccg gcattgttga
     1441 tgaggcagtc gatgtcggaa aattcatcgg ggatgccgtt caaggcgttt tccaccgact
     1501 cgcggcacga cacgtccatt tccaaagggt aaaacaaagc acccaattca tccgccaaag
     1561 cctgaagcct gtccgcacgg cgcgccgcac cgataacgcg gtatcccgcc ccgacaaacg
     1621 tgcggcacat cgcttcgccg aagcctgccg aagcaccggt aattaaaaca gccattgttt
     1681 ttcctttctt tctcgtttcc caaacctgtt tcaggtatca taacaccctt cagacggcat
     1741 cgcgccggct gaaaacgaca acaaccgtac caccacgaca ggacacattc aacatgaaaa
     1801 cccaaatcag ccttgccgca accgccctcg ccctattcct ttccgcctgc ggaaacggcg
     1861 gcgcgcccgc acagcccaaa ggcgaaattt ccgaaaaccg caccgccgcg ttcaaatcta
     1921 tgatgcccga cttctcgcgt atgggcaaaa tggtcaaagg cgaagagcct tacgatgtcg
     1981 aaaaattcaa acaggcggcg gcggcgtttg ccgaaagcag caaaaaaccg ttcacacttt
     2041 ttgagtccga tccgcaaggc aacggccgcg ccctgcctgc cgtttggtcg gatggtgcaa
     2101 aatttgaagc cgaaaaaaca aaattcgccg ccgccgtcga aaaactcaac gccgccgccc
     2161 aaaccggcaa actggacgaa atcaaagccg cctacgggga aaccggcgca agctgcaaat
     2221 cctgccacga cagtttccgc gcaccggaat aacatccgaa acaaaacaat gccgtctgaa
     2281 gccttcagac ggcattttct ttgtcatttc ggcacacgca aagcaatcgg aaattatctt
     2341 caattcaaaa aatacgccat atcctgattt ttgccttact tactccgcca ttttctattt
     2401 tgatttacca tgcgattgat ttttatcgtc aaatgccaaa atcggaatgt cggcctgacg
     2461 gaacagcgca taaacgggct tgagccaata atcttccgga agggaagccg gtttcaatgc
     2521 gtaaacgtgc acattgggaa aatccttcat cgtcaacgcc gcaccgctga aaaaggtata
     2581 aatttcatac ctcgtatgcg ggtttttctt aatttcgcgc aaaatatagt cttcgatgac
     2641 atagggcgaa tttaacgcgg ttacgccgga aagcccgtag gtctggcggg gatgcggcgc
     2701 gacatattgt atgttgaaat tttttgccgc cttttccgaa atttccttca tctctttgtc
     2761 gggcgaaccc aaaagtatcc gcaccgtgcc gcccgtttcg tcccccgcct tcagttcgga
     2821 cgcatcgaac agcggcaggt aagtcatctt gcggcggccg tcgtccataa tgtttttcaa
     2881 acccttgaat atcgtgtaat gctcgtcgga agcattgcgg gttttggcga tgctccaatc
     2941 cccgaccatc attcgggcaa aattccgctt aatcgccccg tttacataaa actcatcgcc
     3001 caaatagctg ctctcccgta tcaggttgtt tgtgccgtcg tcaaaggtct tgatttccgc
     3061 atccgggtaa gtgctcaaaa aggcggcgat gctgactttt tccaaactcg ccaaataaat
     3121 ccgcctgacc ttcggcagca gcatcgactt caccttcagc tccgccatcg tcggaatgaa
     3181 attaaacgat ttgttcaaac cgtagggcag gtagaaaaaa tacgcccgct ccgccttatc
     3241 ctttatctga ttgaaataat aatcgtattt ttcattcctg ttttcagaca tcagcaccac
     3301 ataaaaccgc tcccccggat gctgcgccat aatcctttct gccaccttca tctgcaatat
     3361 ggtatagcag aaaatcagat tgacgggttt cccctcttca ttgaagagtt tgtccttcag
     3421 cagggaaacc gcgttccttt ccccctgatt tacccggtca aacgtataaa atatcccgaa
     3481 gcaaaaaaca atcaggcaca acacggtcaa acagactttt ttcaacccca tatccctaaa
     3541 aactccattc cgacaaattg aacatacgcc ccgagtgtcc cggtgcggaa aatgcggaat
     3601 tttagcagat attccgacag ttgccgaata ttaaaattaa ttttaccgaa aatgtgccgc
     3661 cgcacatcag gcacaacgcc gccgacgccg ccgccaaacg cgggaaagcc atttccccct
     3721 tcctttcccg ccgccgcgtt caaacacgcc aagatcgaac aaagcgcgaa caaagccgcc
     3781 aaaatatgcc ataatagccg cgcacattcc ccgcgcaatg tgtttcaaaa gaaaaaacca
     3841 acctacaaga gaataatcct atgactcaaa aatccaccat tgtttatacc cataccgacg
     3901 aagcccccgc gctggcgacc caatcgctgc tgccgattgt gcaggcgttt gcccgccacg
     3961 ccgatattga tgtcaaaact gccgacattt ccctgtccgg ccgcattttg gcggcattcc
     4021 ccgaatatct gaccgaagcg cagcgcgtac ccgacgcgct tgccgaattg ggcgaactgg
     4081 tgaaacaacc cggtgcaaac gtaatcaaac tgccgaacat cagcgcatcc gtacctcaac
     4141 tgaccgccgc gattaaagaa ttgcagtcta aaggctttgc cgttcccgac tatcctgccg
     4201 accctcaaac cgatgaagaa aaagccgtac gcgaacgcta cgaccgcatc aaaggcagcg
     4261 cggtaaaccc tgtcctgcgc gaaggcaact ccgaccgccg cgcgcctaaa gcggtgaaaa
     4321 actttgccaa aaaaaatccg cacagcatgg gcgcgtgggc gaaagactcc aaaacccacg
     4381 ttgccaccat gcaaagcggc gactttttcc ataacgaaca atccgttacc gtacccgatg
     4441 cgacttccgt atccatcgtg ttcaccgaca aacaaggcaa taaaaaagag ctgcgcgagc
     4501 ctgttgccct gaaagccggc gaaatcattg acgcggccgt aatgagcaaa aaagccctgc
     4561 tcgccttcct tgccgaacaa gtgaaagacg caaaagctaa aggcgtattg ttctcgctgc
     4621 atatgaaagc cacgatgatg aaagtgtccg acccgattat cttcggacac gccgtcaaag
     4681 tgttcttcgc gcctgtattt gaaaaattcg gcggcaaact ggctgccgca ggcgtcaacg
     4741 tcaacaacgg cttcggcaac ctgattgcca atctggacaa actggatgcg gacacccgcg
     4801 ccgccgtcga agctgaaatc accgccgttt acgccgccaa cccggatttg gcgatggtcg
     4861 attccgacaa aggcatcacc aacctgcacg ttcccagcga tgtcatcgtt gatgcttcta
     4921 tgcctgcgat gatccgcaat tccggccgta tgtgggacaa aaacggcaaa gcgcaagaca
     4981 ccaaagccgt gattcccgac agcagctatg ccggtgttta ccaagcaacc atcgatttct
     5041 gccgcgaaca cggcgcattc gatccgacaa caatgggtac tgtgcccaac gtcggactga
     5101 tggcgcaagc ggcggaagaa tacggctcac acaacaaaac ctttgaaatc gaagccgacg
     5161 gtcaggttca agtgattgat gcggcgggca atgtcctgat gcagcacgac gttgaggcag
     5221 gcggcatctg gcgcatgtgc caaaccaaag acgctcccgt caaagactgg gtacaactcg
     5281 ccgtcaaccg cgcccgcctg agcaacacgc ccgccgtgtt ctggctcgac gaaaaccgtc
     5341 cgcacgacaa gagtctgctc gccaaagtca aagcctacct tgccgaattg gataccaacg
     5401 gcctcgacat ccgcgtcctc gcgcccgaag aagccgctaa attcagcttg ggccgtctga
     5461 aaaacggcga agacaccatt tccgtaaccg gcaacgtcct gcgcgattat ctgaccgact
     5521 tgttcccgat tttggaactg ggtaccagcg cgaaaatgct gtccatcgtt ccattgatga
     5581 acggcggcgg tatgtttgaa accggcgcgg gcggttctgc accgaaacac gtccaacagt
     5641 tcctcgaaga aaaccacctg cgttgggact cattgggcga attcctcgcg cttgccgtat
     5701 cgtttgaaca tctggcgcaa aaaaccggca acgccaaagc ccaagtcctc gccgacactt
     5761 tggatgccgc caccgaaaaa ctgctgttga acgacaaatc gcccaaacgc aaagcgggcg
     5821 aactcgacaa ccgcggcagc catttctacc tcaccctcta ctgggcgcaa gaattggcgg
     5881 cgcaagacaa agatgccgaa ctgaaagccg catttgctcc attggcagcc gctttgactg
     5941 ccgacgaagc caaaatcgtt gaagaactct ctgccgtaca aggcaaagcg gtcgacatcg
     6001 gcggctacta cgcagccaat cctgaaaaag ccgcacaagc gatgcgtccg agcgcaacct
     6061 ttaatcaggt actaaacgcc ttataagcac aaaggcaaaa atgccgtctg aacattcgtc
     6121 gttcagacgg cattagttat ccctatctgc ctgattatga ttaaacgccg cacccattgg
     6181 atatatagtg gattaacaaa aaccagtacg gcgttgcccc gccttagctc aaagagaacg
     6241 attctctaag gtgctgaagc accaagcgaa ccggttccgt actatttgta ctgtctgcgg
     6301 cttcgtcgcc ttgtcctgat ttttgttaat ccactataaa tcatataatc tttagttttt
     6361 taccctacgg aaagacaata atatgaaaac tgttccaacc atcctgaccg gcatcctttt
     6421 ggcaaccgcc cttcccgcat ccgcacacgg tatgcataag agcaaacccc tggctatgga
     6481 cgagctgccg ccgatttgcc aacaatattt caaacgcgcc gaaacctgtt acaacaaagc
     6541 cggaaacaaa gcagattttg cacgcaacaa caccaaattc ctattccaag ccctacctgc
     6601 cgccgatttg ggacagcgta agcaaatgtg ccaaatcgct atggattcgt ttgcagaaaa
     6661 aaacccgcaa tctgaattgc gaataaagcc gcatcagcaa tagcaatgcc gtctgaaaca
     6721 aaggttcaga cggcattctc acatcaaaac gcaccggctg ataaaacccg ccttccccgc
     6781 atccctgacc cgaacaggat taaacggagt aacggtttcg gccattatgg caagtcccca
     6841 tcccaaaaat gaccatataa attaatacca taaaacattg ataaaaagcg tctgccgatt
     6901 gcggtttgtt gcagggcgtt acggaaaatc atcgtaaacc gatgattttt a
//
LOCUS       DAXFWM010000088         6593 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000088 DAXFWM010000000
VERSION     DAXFWM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.088"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1341
                     /gene="mafB"
                     /locus_tag="ACFI8B_001969"
     CDS             <1..1341
                     /gene="mafB"
                     /locus_tag="ACFI8B_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC3835109.1"
                     /translation="ARDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDR
                     ADEANRKMFEHNAKLDRRGNSMEFVNGVAAGALNPFISAGEALGIGDILYGTRYAIDK
                     AAMRNIAPLPAEGKFAAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPF
                     AKVKNLTKAAKPGKAAVSGDFSKSYTCSFHGSTLVKTADGYKAIAHIQAGDRVLSKDE
                     ASGKTGYKPVTARYGNPYQETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKA
                     GSRLLSESGKTQTVRNIVVKPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPTK
                     LKPTERYNRQTHYGGSQTDGARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPL
                     VKHYYEHGGHSMSNADRAKHARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            1331..1879
                     /locus_tag="ACFI8B_001970"
     CDS             1331..1879
                     /locus_tag="ACFI8B_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC3835110.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            2176..2529
                     /locus_tag="ACFI8B_001971"
     CDS             2176..2529
                     /locus_tag="ACFI8B_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835111.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            2519..2842
                     /locus_tag="ACFI8B_001972"
     CDS             2519..2842
                     /locus_tag="ACFI8B_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC3835112.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            2901..3128
                     /locus_tag="ACFI8B_001973"
     CDS             2901..3128
                     /locus_tag="ACFI8B_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835113.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDGQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            3255..3798
                     /locus_tag="ACFI8B_001974"
                     /pseudo
     CDS             3255..3798
                     /locus_tag="ACFI8B_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            3900..4664
                     /locus_tag="ACFI8B_001975"
                     /pseudo
     CDS             3900..4664
                     /locus_tag="ACFI8B_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
     gene            4661..4996
                     /locus_tag="ACFI8B_001976"
     CDS             4661..4996
                     /locus_tag="ACFI8B_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835114.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            5044..5568
                     /gene="mafB"
                     /locus_tag="ACFI8B_001977"
                     /pseudo
     CDS             5044..5568
                     /gene="mafB"
                     /locus_tag="ACFI8B_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
     gene            5546..5827
                     /locus_tag="ACFI8B_001978"
     CDS             5546..5827
                     /locus_tag="ACFI8B_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835115.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            5883..6482
                     /gene="mafB"
                     /locus_tag="ACFI8B_001979"
                     /pseudo
     CDS             5883..6482
                     /gene="mafB"
                     /locus_tag="ACFI8B_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
BASE COUNT         2157 a         1311 c         1424 g         1701 t
ORIGIN      
        1 gcacgagacg aatacaccta tcacgtcaac ggcacagccc gcagtatcaa actcaatccg
       61 accgacaccc gcagcatccg gcaacgcata tccgacaact acaacaacct cggcagcaat
      121 ttctccgacc gcgccgatga agccaacaga aaaatgttcg agcacaatgc caagctcgac
      181 cgccggggca acagcatgga gtttgtcaac ggcgtcgccg ccggcgcgct caaccccttt
      241 atcagcgcgg gcgaagcctt gggcataggc gacatactgt acggaacgcg ctatgccata
      301 gacaaagccg caatgcgcaa catcgccccc ttgcccgccg agggcaaatt cgccgccatc
      361 ggcggcttgg gcagcgtggc gggctttgaa aaaaatacgc gcgaagccgt tgaccggtgg
      421 atacaggaaa accccaatgc cgccgaaacc gtcgaagccc tggtcaacgt cctgccgttt
      481 gccaaagtca aaaacctgac aaaggcggca aaaccgggga aggctgcggt tagtggggat
      541 ttttctaaat cctacacctg ctccttccac ggcagcacct tggtcaaaac ggcagacggc
      601 tacaaagcca ttgcccatat tcaagccgga gaccgcgtcc tttccaagga cgaggcaagc
      661 gggaaaacag gatacaaacc cgttaccgcc cgatacggca atccgtacca agaaaccgtt
      721 tacattaaag tttcagacgg catcggtaac agccaaaccc tgatttccaa ccgcatccat
      781 ccgttttatt cggatgggaa atggattaaa gcggaagatt tgaaagcggg aagccggctg
      841 ttatccgaaa gcggcaaaac ccaaaccgtc cgcaacatcg ttgtcaaacc aaaaccgctc
      901 aaagcctaca atctgaccgt tgccgattgg catacctact ttgtcaaagg cgataaggcg
      961 gaaacggaag gggtttgggt tcataatgat tgtccaacca aactaaaacc gacagaacgt
     1021 tataatcgtc aaacgcatta tggaggttct caaacagatg gagcaagggc tcaggcagca
     1081 agacaggcag gagaaggtaa accttgtcca acttgtggta gaattcagat atttggtacg
     1141 aaaaccgcac caagtccgca acatgaaccc ccattagtaa aacattacta tgaacatggt
     1201 ggtcactcta tgagcaatgc tgatcgagcg aaacatgcca gggaaagcat aaaaggaact
     1261 caatgtttaa catgtcaaag gaaagagggt gcgatgatgt caagatattc gagagaacag
     1321 gctaaaaagc atggactata accaaactgt tttatctcac ttgcaaaaat tttggaaaca
     1381 tcatgatatt aaaggattta cttggacttt aggaagaatt gttgaagaac ttcccgattt
     1441 ccaagttttc caagtgattc ctaatcatga agatgagcct tgggtttatg tcagctctgg
     1501 gataggtcaa tttttagggc aggagttttt tatcatcagc ccgtttgaaa cacctgaaca
     1561 tattgagacc ttggcaatgc tggcatctgc aagtatgcat tatcctgatc aatttcaatt
     1621 agggaaaacc gtcaatatcg gtagaccgtg ggtcgagcaa tcatccttcc ggcattttct
     1681 gatttctctg ccttatcctt atgggcaaga gttggaatat atggataatg tccgattttt
     1741 ctggttattg ccaattaccc agacagaaag acttttcttg aatactcatt cggtagaaga
     1801 attggaaacg aaatttgatg aagcgggtat tgattattta gatataaatc gtgcgagtac
     1861 tgtctggcag gcaggataaa aattcggcaa tcaaaatgcc gtctgaaaca agtttggact
     1921 ttcagacggt attctgatga atggaacagg atactgggaa ctgagttctt caaaaatcct
     1981 atctccgtcc cttatacgtc ggtgctttag tcaaaacggc agacggatga aaaattattg
     2041 cccgtatcct tgccaaagat gaagcaagat gaagccaggt aattgaatcg gtacaagcgc
     2101 aatgtccgac ttgttctgca aaacagggag ggagattaag ccaatattca aaagaacaga
     2161 aaaagaaact gagtaatggg aataaaaaat gaacaaataa taatttgtaa aaaatataat
     2221 acagaaatat atcctgtttc cgatgtttca aaaatcggtg ttgctgaaaa tgtcaaacag
     2281 acaggcctgt atccgattaa cgggttaagg caccgtccca aaggtgatac taacggttgg
     2341 tatatctggg ccggtgaaaa cttctcctat gataaaaatt ttttcctgcc cttgcatacg
     2401 ttccatttgc aaatatggcg acccgaaatc atcccctttt tgacacttcc gcccggttac
     2461 cgttttttaa ttggggaaaa cggttatgaa gacgtatggt ttgatgaatt gctattaaat
     2521 gataattgac gaaaaatcta cttcagatgt actgaaacaa tatcaagaac tagggtttga
     2581 tttgacaaaa cctatgataa tagagttttt tataggaggt tctcaaaaaa atctgcaatc
     2641 aatagaaagc caactcattt cttatcggca agattttcaa atcagtattg aacaagatga
     2701 attcggagaa atgtggactt gctattgttc cgtcaatatc gtcccttccc ttgaaaacat
     2761 tttagctatt gaaactacgt tatttggtat tgcacaaaaa aatgattgta attatgaagg
     2821 ttttggttcg tatgggaact aacaaaaagt gtaggttggg ttgaaaaaac catattccca
     2881 ttaaagatta gggtgtgtct atggacataa aattcaatac tttgggggtt atcctgaatg
     2941 gggtcaatcc tgaagaaaaa ttcataaaaa ttattgatgg ccaggaaaat accggtggat
     3001 ttctgatact gttatcttca aatgacaaat tctcatcatt tgacagttac gatgactggg
     3061 tcgaaaactt agaaatatta aaagaatacc tgcaagaatc tcattggata ataaagtggg
     3121 tcggatagtc ggcaaacgta gtccgtttga aacgctttta cttttcagac ggactttcta
     3181 ccaatgggaa caaggcgttt tgtctcaatc cggtttatga ttattcgata cagtgatggg
     3241 aagctaagaa aaatatgatt aatcatattg aaaaatatat aggcactatt tctcatggct
     3301 cgaaaagcga ttcgggaagt cagtacaaat taaatatcgc agctattccc tcttccccta
     3361 acagggattt aaaaacttat attactttag ggttaagcaa acatgatctg aattataaaa
     3421 gcagatttga aatacttttt gtatgttctt taaaatatga tgaaaatcag attttcccat
     3481 ttttaagatg gttagcagaa accatcattg agaataaaaa aaatccttct tcgggggcag
     3541 gttgtttatt tgcctagaag cattgttaat tcaacaaaaa tggacgcact gtatgtttcc
     3601 gccccatttt atttcgacga tgatttccaa gtctgttatg gcgaacacta caatattgtt
     3661 ttccctttgc ttgtcccctt gtataaacag gaagccgaat tggtggaaaa aaagggttgg
     3721 aatgcttttg agcagttctt gttggataat gaagttggca acctttcgga tatgaatagg
     3781 aagccgtttg tttggtaagg gctttcttaa atttgattaa aggtcgtctg aaaccgtgtt
     3841 taactttcag acaacctttc cttcatttga aacagaatat tgaaaactaa gttcttcaaa
     3901 aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac ggctacaaag
     3961 ccatcgccca tattcaagcc ggagaccgcg tcctttccaa ggacgaggca agcggagaaa
     4021 cgggatacaa acccgttacc gcccgatacg gtaatccgta tcgggaaacc gtttacatta
     4081 aagtttcaga cggcatcggc aacagccaaa ccctgatttc caaccgcatc catccgtttt
     4141 acagtcaagg aaaatggata caggcaggtc gtctgaaaaa aggcgacacc ctgctttccg
     4201 aaagcggtgc aaaacagacg gttcaaaaca ttaccctcaa acagcagccg ctcaaagcct
     4261 acaacctgac cgttgccgac tggcatactt acttcgtcaa gggcagtcag gcggaaacgg
     4321 aaggggtttg ggttcataat gattgtccgt atggaaaagg caaccaacga tataaagatg
     4381 ctccttatca tggtaaaaat gataattctg tgaaaagtag agcgccaaca aatgggcaag
     4441 ctgtccttga taactctgtt caagttaaat cgacatcttc tcaaagagtt ggagttgata
     4501 aaaccaataa tgaaatcgtt gtattaaacc aaactagaat ttttaatgac ggttctgctg
     4561 agtatcatgg gcatgtaagg aactggaaaa atttacatac tgatcaacaa aatgctttaa
     4621 agaaagcagg attggttaat tcaaaaggaa aaatcaaaaa atgatggctg ataaggaaca
     4681 agtagaaaag atgatttatt ctgaaaataa agattgtgtt attgatggta tgctcggtat
     4741 gaccttcagc tcggaggagg atgaaatccc ttggatttcc gagaaattga cggagttgtc
     4801 gaagcataaa gacttggata ttgccagatt gtcattaacc tgtttcggac atctcgccag
     4861 gatgcatgaa aatatcggcg attgcgataa agtgattgct ttattgcgct caaaacaagg
     4921 taatccggat tttcaaggtt ttgccgaaga tgcgttagac gatatttcct tctttatatt
     4981 taaaaagcgt ccgtaagcgc ggattaaacc cggaaatccg atgcgtctga aagtacattc
     5041 cggctttcag acggcatcgg taacagccaa accctgattt ccaaccgcat ccatccgttt
     5101 tattcggacg gcaaatggat taaagcggaa gatttgaaag cgggaagcag gctgtttgct
     5161 gaaaacggtg cagagcagac cgttcaaagc gttaccgtca aaccggaacc gctcaaagcc
     5221 tacaatctga ccgtcgccga ctggcatact tatttcgtca agggtagtca ggcggaaacg
     5281 gaaggagttt gggttcataa tgattgtccg cctaaaccaa aaccaaccaa tcatgcccaa
     5341 caaagaaaag aagaagctaa aaacgattct catcgaagtg tgggagattc caatcgtgtc
     5401 gttcgcgaag gaaagcaata tttagattcc gacacaggaa accatgttta tgtaaaagga
     5461 gataaagtgg ttattctaac tcctgatgga agacaggtaa ctcaatttaa gaactcgaaa
     5521 gccaatacgt caaaaagggt aaaaaatggg aaatggacac caaaataatc aatcttcctt
     5581 agaaaaaaga attttttatc tggagcattc ggggcagtat ctgatgattt gcgccctatc
     5641 ggattactcc caaaataaac atactgtcgt tatggcgaat ttcctctatc caaatgaaaa
     5701 aatggattgg agaaatttag atgatttgtt caatgagttg gttttagagg aattacagtc
     5761 ttcatttatg gattggtatc caactattga aaaggcaatt agccatcatt tagaagactt
     5821 ttcgtaacaa gcataaatca aaaagtcgtc tgaaactgct tttaattttc agacgacctt
     5881 tccccatatc ggcggattat gaatttacag ctatcgggaa attgagttat ctctctgata
     5941 gagaaaattc catacgcgaa gccgttgacc ggtggataca ggaaaatccc aatgccgccg
     6001 aaaccgtcga agccgtcttc aacgttgccg cagcagccaa agtcgcgaag ttggcaaagg
     6061 cggcaaaacc agggaaggct gcggttagcg gggattttgc tgattcttat aaaaagaaat
     6121 tggctttgtc tgatagtgcg agacagttat atcaaaatgc aaagtataga gaagctctag
     6181 atatacatta tgaagattta attagaagaa aaaccgatgg tacatcaaaa cttgttaacg
     6241 gtagagaaat tgacgctgtt acgaatgatg ctttaataca agccaaaaga acaatttcag
     6301 caatagataa acctaaaaat ttcttaaatc aaaaaaatag aaagcaaatt aaagcaacca
     6361 tcgaagcagc aaaccaacag ggaaaacgtg cagaattttg gtttaaatac ggtgttcatt
     6421 cacaagttaa gtcatatatt gaatcaaaag gcggcattgt taaaacaggt ttaggagatt
     6481 aaacatggca tcatatgttt ccataaaagg atggattgaa tgctctgacg atgatataaa
     6541 aatcatccaa gaaaacatta ataatttttg gaataattgt ccctttaata tcg
//
LOCUS       DAXFWM010000089         6312 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000089 DAXFWM010000000
VERSION     DAXFWM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.089"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(71..1309)
                     /locus_tag="ACFI8B_001980"
                     /pseudo
     CDS             complement(71..1309)
                     /locus_tag="ACFI8B_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1489..2862)
                     /locus_tag="ACFI8B_001981"
     CDS             complement(1489..2862)
                     /locus_tag="ACFI8B_001981"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3835116.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3076..4080
                     /gene="dusB"
                     /locus_tag="ACFI8B_001982"
     CDS             3076..4080
                     /gene="dusB"
                     /locus_tag="ACFI8B_001982"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC3835117.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4093..4242
                     /locus_tag="ACFI8B_001983"
     CDS             4093..4242
                     /locus_tag="ACFI8B_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835118.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4269..4508
                     /locus_tag="ACFI8B_001984"
     CDS             4269..4508
                     /locus_tag="ACFI8B_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC3835119.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4511..5047
                     /gene="ruvC"
                     /locus_tag="ACFI8B_001985"
     CDS             4511..5047
                     /gene="ruvC"
                     /locus_tag="ACFI8B_001985"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC3835120.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5098..5967
                     /locus_tag="ACFI8B_001986"
     CDS             5098..5967
                     /locus_tag="ACFI8B_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC3835121.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
BASE COUNT         1372 a         1620 c         1871 g         1449 t
ORIGIN      
        1 tgtgtgaatg gtgaaataaa aatgccgtct gaacccgatt ggattcagac ggcattttgt
       61 atggcaggat ttattcgctt tccaactgac cgacccattc tgacaaggca gtcaggcgtt
      121 gttcggattt cgccacgaac gggttttcgg tatcccacgc gtagcctgcc aaaatcgaag
      181 aaagcatacg gctgatttcc gggctgcggt cgggcgcgta cacgacgttt tggaagcgga
      241 aatcatacca gccgtccaca taggtgcgga acgcgtctac gccgatcatc aggggttcgg
      301 caaattcggt ttgccaatcg gcggcttcgc ctttgagttg ttttgtcagc agatcggcag
      361 caagttcggc ggagtgcagc gcgatggtta cgcccgacga gaacacgggg tcgaggaact
      421 cggcggcatt gcccaacagc gcgaaatgcc tgccgtgcag tgatttgacg ttggcggaat
      481 agccttggat ggagcggaac ggaaaatcgt tttcccaaac ggctttgtcc aaaatttcgc
      541 ttaacatcgg gcattcgtaa acaaattttt tcaacaccgt ttccgattcg ccggcaagtt
      601 tgtcgggtgt gccgaccacg ccgacggaac agcggttgtc gccgaaggga atcagccaaa
      661 tccacacgtc gcggttgttg cggatgggtg gtaatcagga ttttgttgcg gtcgaatttc
      721 gggtgggtaa tgttgtcgtc gatgtgcgtg aaatgcgttt ggcgcggcgg caggtgcgag
      781 ggcgtttcca agtttagcag gcgcggcagc acgcgtccgt agccgcttgc gtccaagacg
      841 aatttcgcgg tcagttcata gctctcgccg gtgtcggttt cgatgttcaa gcgggcaaaa
      901 tcgccgctgt tgtcgaacgc ggttacgccg tgcccgaaac gtacttcaac gccttgtttg
      961 gcggcttctt caatcaggat tttgtcgaac acggcgcggc ggacttggta aaccgtgccg
     1021 gggccgtctg aaaatttatc ggtgaaatca aactcggtat agcggctgcc ccacgaaaac
     1081 gccgcaccgt ttttcaactg aaagccgggc ccggtgcgaa cggcatcggc aaaaccggct
     1141 tcttccagca tttccataca gtgcggcagc aggctttcgc cgatgacgaa gcgcggaaag
     1201 tgctgttttc caacacgcag acttgataac cttttttgcg gagcagggcg gatgcggccg
     1261 aacctgccgg acctgcgccg attacggcga catcgaattg ggtggacatt gggaaatcct
     1321 gatggtttga gtggaaaagg aaatgatatt gtacaggcag caaatgccgt ctgaaactgt
     1381 ttgcggaaga aatttatccg gtgcgggcgg ttgctcccaa tgcgtcagtc cggtttgaaa
     1441 aatgccgtct gaaacgggaa atgttcagac ggcatttgat tttcagggtt attttacgcc
     1501 gtaacgcggt tggagcaggg cgcacgtccg gtgtccccgg cggcttcggc ggctgcgccc
     1561 ggcaattttt ccgcaggccg cgcccgcatc gtttgccgtg gtttccggcc gggtattttt
     1621 ttcgcgctga gccctggcgg attcgtagcg gttgcgttgt ctcggttcgc ttgtttcggt
     1681 tttttccggt tttgagcctt cctgttccca ccatcggggc tcgaagcctt cgatgcgttc
     1741 gatgagcagc ttgttgccgg tcagttcttt gatggcctca aacattttct gttcggattc
     1801 gtccatcagg gaaatcgcca cgccgtccgc accggcgcgc cccgtgcgtc cgatgcggtg
     1861 gatgtagtct tcgggctggg cgggcatttc gtaattgatg acgaagggca gttcggcgat
     1921 gtccagccca cgcgcggcga tatcggtggc gacgaggacg cgcaggctgc cgtctttgaa
     1981 ggcgttgagt gtttcgagcc tgctttgttg ggaacggtcg ccgtgtatcg cctgtgcgga
     2041 caggttgcgg cgcaccagtt cgcgcgttac gcggtcgacg ctttgtttgg ttttgcaaaa
     2101 gacgataact tggttcatat gcagatcgac aatcagccgt tcgagcaggt tgcgcttttg
     2161 gaaggtatcg acggcgatga tgtgttgttc gacgttggcg ttggtggtgt tttgggcggc
     2221 aacctcgacg gtttcgggcg cgttcatgaa gtcttgcgcc agtttgcgta tcggggcgga
     2281 gaaggtggcg gaaaagagca gggtttggcg ttggcggggc agcatctgca tgattttgcg
     2341 gatgtcgtcg ataaacccca tatccaacat gcggtctgct tcgtccagaa cgacgatttc
     2401 ggctttgttt aaactgatgt ttttctgttt cacatggtcg agcagccgtc cgacggtggc
     2461 gacgactatt tcgcagccgg cacgcaggtc ggcggtttgt ttgtccatgt tgacaccgcc
     2521 gaagaggacg gtatgccgca gcggcaggtt tttaatgtag ccctgcacgt tttggtcgat
     2581 ttggtcggca agttcgcgcg tcggggtcaa cacgagcata cgcacggggt gcattgcagg
     2641 cgaggtgctg gcggtggcgt aacgtttgag gcgttccaga ctgggcagca taaaggcagc
     2701 ggttttgcct gtgccggttt gcgcggcggc aagcaggtcg tgtccggcca gtgctttggg
     2761 aatggccgcg gcttggatgg gcgtcgggtt ttcgtaacct tgcgcggtca gtgcggaaac
     2821 cagttccgta cccaaaccta aagaggaaaa tggattactc atgattgtag tctttctttc
     2881 agaccttatg ccgtctgaag cgggaaaccg ataggacggg gaaataaact gcccgcctgt
     2941 gaacggcggg gacgggaatt tgtgtgcgga ttatgccata aaacggtgcc cgccccaatc
     3001 gcgggcgcgt ccgcagattg gaaatcctgc ttaaaaaatg tacaatggcg cacttttttg
     3061 aaacgcgggc ccattatgca catcggcggt tattttatcg acaaccccat cgcacttgcg
     3121 ccgatggcgg gcattaccga caaacccttc cgccgcctct gtcgggcgtt tggcgcaggt
     3181 tgggcggtgt gcgaaatgct ggccagcgat ccgacgctca ggaataccgg aaaaaccctg
     3241 caccgcagtg attttgccga tgaaggcggc atcgttgccg tgcagattgc cggcagcgac
     3301 cccgaacaga tggcggatgc ggcgcgttac aacgtcggac tcggggcgca ggtcatcgac
     3361 atcaatatgg gctgccccgc caagaaagtg tgcaacgtcc aagccggtag cgcgctgatg
     3421 caggacgagc cgctggttgc cgccattttg gaggcggtgg tcaaggcggc gggcgtaccc
     3481 gttaccctca aaacccgttt gggttggcac gacgaccatc aaaacctgcc cgccatcgcc
     3541 aaaatcgccg aagattgcgg cattgccgcc cttgccgtcc acgggcgcgc gcgcacgcaa
     3601 atgtacaaag gcgaggcgcg ttacgaactc atcgccgaga ccaaaagccg tctgaacatc
     3661 ccggtctggg tcaacggcga catcacttcg ccgcaaaaag ccgccgccgt cctcaaacaa
     3721 accgccgccg acggcatcat gatagggcgc ggcgcgcaag gcaggccgtg gtttttccgc
     3781 gatttgaagc attatgccga acacggcgtt ttaccgcctg ccttgagttt ggcagaatgc
     3841 agagccgcca ttttgaacca catccgcgcc atgcacgcgt tttatggtga gaccgtcggt
     3901 gtgcgcatcg cacgcaaaca cataggctgg tacatcggcg aaatgcccga cggcgaacag
     3961 gcgcggcgtg aaatcaaccg cttggacaat gcggcggcgc aatacgacac acttgccggt
     4021 tatcttgaaa ggcttgccgg aaaaaccgac cgttgggcgt gcggctatcg ggaagggtag
     4081 ggcagtattg ccatgccgcc gttcgggttc ggacggcatc tgtctgcatg gttcggaggt
     4141 cgggcggaat cccggccggc gaatgtcgaa aacaataaat gccgtctgaa aacgagtgga
     4201 acgggtttcg ccaaaaggct ttcagacggc attccgtctt aaaaacattt caatcaaaag
     4261 gatatgcgat gaaccctgcg actgcggaca ttgcgcaatg tatcgagcag aacttgaacc
     4321 aatatttcaa agacctgaac ggcaccgaac cttgcggcgt gtacgatatg gtactgcatc
     4381 aggtggaaaa gccgctgctg gtgtgcgtga tggagcaatg cggcggcaac cagtccaaag
     4441 cctccgtgat gctgggactg aaccgcaata ccttgcgtaa gaaactgatt caacacggtt
     4501 tgctgtgaat atgtcggcaa ccgtccgtat cttgggcatc gacccgggca gccgcgtaac
     4561 gggtttcggc atcatcgatg tcagggggcg cgatcatttt tacgtcgcct ccggctgcat
     4621 caaaacgcct gccgatgagc ctctggcaga caggattgcc gtgattgtgc ggcatatcgg
     4681 cgaagtcgtt gccgtttaca agccgcaaca ggcggcggtg gaacaggtgt tcgtcaacgt
     4741 caatccggca tcgacgctga tgctcggtca ggcgcgcgga gcggcattgg cggctttggt
     4801 cagccataag ctgcccgttt cggaatacac ggccttgcag gtcaaacagg cggtggtcgg
     4861 caaaggcaag gcggcgaaag aacaggtgca gcatatggtg gtgcaaatgc tgggactttc
     4921 gggaacgccg caggcggatg cggcggacgg tcttgccgtc gcgctgaccc acgccttacg
     4981 caaccacggg cttgccgcca aactcaatcc ttcggggatg caggtcaagc gcggaaggtt
     5041 tcaatagttt cagacggcat ttgtattttg ccgcctgaaa agaaaatgtg taccgagatg
     5101 aaatttatat tttttgtact gtatgttttg cagtttctgc cgtttgcgct gctgcacaag
     5161 attgccggcc tgatcggttc gcttgcctac cttctggtca aaccgcgccg ccgtatcggc
     5221 gaaatcaatt tggcaaaatg ttttcccgaa tgggacgaag aaaagcgtaa aaccgtgttg
     5281 aaacagcatt tcaaacacat ggcaaaactg atgctcgaat acggcttata ttggtacgcg
     5341 cctgccaaat gcctgaaatc gctggtgcgc taccgcaata agcattattt ggacgacgcg
     5401 ctggcggcgg gggaaaaagt catcatcctg tacccgcact ttaccgcgtt cgagatggcg
     5461 gtgtacgcgc ttaatcagga tgtcccgctg atcagtatgt attcccacca aaaaaacaag
     5521 atattggacg aacagatttt gaaaggccgc aaccgctatc acaacgtctt ccttatcggg
     5581 cgcaccgaag ggctgcgcgc cctcgtcaaa cagttccgca aaagcagtgc gccgttcctg
     5641 tatctgcccg atcaggattt cggacgcaac aattcggttt ttgtggattt tttcggcatt
     5701 cagacggcaa cgattaccgg cttgagccgc attgccgcgc ttgcaaatgc aaaagtgata
     5761 cccgccattc ccgtccgcga ggcggacaat acggttacat tgcaattcta tcccgcttgg
     5821 aaatcctttc cgagtgaaga cgcgcaagcc gacgcgcaac gtatgaaccg ctttatcgaa
     5881 gaacgcgtgc gcgaacatcc ggaacaatat ttctggctgc acaagcgttt caaaacccgt
     5941 ccggaaggca gccccgattt ttactgacta cataaaatta caaaacaaat caggcgtttc
     6001 agatcaaaaa ccccgattgt ttttgggaat ttgaaacccg ggttgtacaa acaggatttg
     6061 ccggacggtt ttaacggttc agttgtttgt aaaaacaatg cttttttaaa attgacaaaa
     6121 aacgaaatcg gttttaaagg cttattccga gaacaaaggg gagtggatgc cgaaaacccg
     6181 gttaatatat tatagtggat taacaaaaac caatacggcg ttgcttcgcc ttagctcaaa
     6241 gagaacgatt ccctaaggtg ctgaagcacc aggcgaatcg gttccgtact atttgtactg
     6301 tctgcggctt cg
//
LOCUS       DAXFWM010000090         6292 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000090 DAXFWM010000000
VERSION     DAXFWM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.090"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(206..439)
                     /locus_tag="ACFI8B_001987"
     CDS             complement(206..439)
                     /locus_tag="ACFI8B_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC3835122.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(457..591)
                     /locus_tag="ACFI8B_001988"
     CDS             complement(457..591)
                     /locus_tag="ACFI8B_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835123.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            complement(605..805)
                     /locus_tag="ACFI8B_001989"
     CDS             complement(605..805)
                     /locus_tag="ACFI8B_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFC3835124.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEF
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(977..1852)
                     /gene="xerD"
                     /locus_tag="ACFI8B_001990"
     CDS             complement(977..1852)
                     /gene="xerD"
                     /locus_tag="ACFI8B_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC3835125.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRGSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(1904..2344)
                     /locus_tag="ACFI8B_001991"
     CDS             complement(1904..2344)
                     /locus_tag="ACFI8B_001991"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC3835126.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            2665..3603
                     /locus_tag="ACFI8B_001992"
     CDS             2665..3603
                     /locus_tag="ACFI8B_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC3835127.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3732..4025
                     /gene="hfq"
                     /locus_tag="ACFI8B_001993"
     CDS             3732..4025
                     /gene="hfq"
                     /locus_tag="ACFI8B_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC3835128.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            4110..4676
                     /locus_tag="ACFI8B_001994"
                     /pseudo
     CDS             4110..4676
                     /locus_tag="ACFI8B_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            4730..5104
                     /locus_tag="ACFI8B_001995"
     CDS             4730..5104
                     /locus_tag="ACFI8B_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC3835129.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            5114..5608
                     /gene="folK"
                     /locus_tag="ACFI8B_001996"
     CDS             5114..5608
                     /gene="folK"
                     /locus_tag="ACFI8B_001996"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC3835130.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(5736..6215)
                     /locus_tag="ACFI8B_001997"
     CDS             complement(5736..6215)
                     /locus_tag="ACFI8B_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC3835131.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
BASE COUNT         1684 a         1874 c         1298 g         1436 t
ORIGIN      
        1 ttattgaaaa aatcaataaa ttaagaattc tcccaaccga caaataaaat aaagaaaggg
       61 tcaatatgca acaccgtaga agattggcaa tttaccaggc atccaaacgt gcttccttta
      121 ccggcaggtc atcagcccca caaaaacgta aagaacgttg atttgaaaaa aatgccgtct
      181 gaagtcctgc ttcagacggc attttttacc gttcgagaaa ctgtttcaac ctgtcctcat
      241 ccaaaaacca gtggcaaatt tcctcatctc ccgccaacaa aacgggaatc agctcattgt
      301 atttttcttc caaaacagga ttttcatcca catcgaccac ttccagcccg aacccgtatt
      361 catcctgaaa aggtttgagt gcatcgcgca ttttgtggca caagctgcaa tattcacgaa
      421 acatcaaggt caatttcatc cgcgtttcct tatctgtcaa tttgcacacg ccaaagcctt
      481 agacgcagca gaatcatggt ctatttggga aaaaacaatg tcttcgagga agatgatact
      541 caagtcctgc caaaaacagc aaaaatgccg tctgaacagt tcagacggca tttccaaaac
      601 cgttttacgc ttgaacgttg atacccgccg taaccgtcgg ttgcgcgttg tgcgccgtca
      661 gaaacgaagc ggcaaactcc ccgcagcagc cgcagcagct cgccacgccc aattgcgact
      721 gcaaatcgcc cattgtggtc gcgccggcag cgatggtttc cttgatttga tggtcggtaa
      781 cggcattgca gatgcagaca aacattttgt gctccgtgtg ttctcaaact atcgtaccga
      841 tagcgacttt tattatcgta cgcaaatata aataaaaacg gttcgcattg caaggtcggc
      901 atacacggtt tgtcttggta attttttatc aagtttgcat tttcgattga tttcttatta
      961 aaccgaagca gaagcctcag tttcgggaat ggtgttcctt aaccacgcta tgcagccgca
     1021 cattggcaac atgggtataa atctgagtcg tgttcaaatc ggcgtgtccc aacatatcct
     1081 gaactacgcg caaatccaat ccgtgccgca ccagatgcgt ggcaaaggcg tggcgcaggc
     1141 tgtgcgggct gatgtgcccg atgcctgccc tgccggcata ttctttgaca atcatccacg
     1201 ccaactgccg ggaaatgccc gtttttttct gactgacaaa caatgcgtcg caactcctgc
     1261 ctttcagcag aagcgggcgt gcctccgtat aatagcgttc cacccaatac gctgactcct
     1321 gtcccatcgg gaccatcctc tgcttgtcgc ccttgcccag cgtggtaata caacccctgt
     1381 ccaaatccac attgccgaaa ttcagcccga ccgcctcgct gacgcgcaag ccggtcgcgt
     1441 acatcaattc gagcagggcc ttatcccgca aaccgtgcgg cgtgtcggta tccggtgcgg
     1501 caagcagtcg ggaaatctgc tgttctgtaa tcagtgtcgg gatgtttctg tcgatttttg
     1561 gcggtttcag caggcgggtg ggattgtccg ccctcatccc ttcccgttcc atccacacat
     1621 acaggcgttt gcacgccgac agtgcgcgtg cctgcgaacc ccgttgctcc ccgtcaacat
     1681 aaaccgccgc cgccaaatct gcttcgtccg catccttcag cgttctgccg cacaaagaca
     1741 gccggcgggc gatcttttcc aaatcccgcc ggtagctgtc caaagtattc cgactgagcc
     1801 gccgatccaa ccacaaagtt tccagcagcc tgtcaatcaa accgttttcc atagcgttcc
     1861 aaaccaatgc cgtctgaatc ttcttcagac ggcatggtcc acatcatcgg gaaagcgttt
     1921 ccaacacttc ctgtgcatga cccgccactt tgactttccg ccactcccgg acaatttcgc
     1981 cgtccttgtt cagaacgaac gtactgcgct cgatgcctaa cgactctttc ccatacagtt
     2041 ttttcaattt gatgacatca aacaggcggc acaccgtttc atccttgtcg ctcaacagct
     2101 cgaaccggaa accctgcttg gcgcaaaaat tctgatgcgc ctttacgccg tcgcgggaaa
     2161 taccgaccac ggtataaccc aatgcctcaa actgttccaa acgcgcatta aaatccaagc
     2221 cttccgtcgt acagcccggc gtactgtctt tcggataaaa atacacgacc aaaggcagat
     2281 gttctgccga atgaaaatcc gcaccgctgc tcgaaggcag ggtaaattca tatttcatat
     2341 ccatagtcct actcccgata ttcccattat tcaaaacggc acgcagacgc accgccgcaa
     2401 ttgccaaacc aaccccgatt ctaccgcccc aaaggacaag gattcaaccg ccggaaacat
     2461 ccaaaccgac acacgacggc atgaaaaata tccatgtcaa accacaaaat atgttccgat
     2521 ttaaaaacag aatgttataa aacccaactc caaaaacaca acaagaccgc ccgcctacgg
     2581 gcagtctcct gtcagacgac atactttaca gatggctgtt ttttcaacaa aataacgcca
     2641 atactcaaaa atacggaacc aaaaatgtcc atccgcaccc tgaaacgcct gccctcatcg
     2701 ctgctgctcg gcctctgcct ttccctgccg tcagcccacc tttttgccga caacgacatt
     2761 ttagggcaat ttttagaaca gaacatgctt acctcctccg atccgataga aatattcgcc
     2821 gaaagcacga tatatcccgc cgatacccag gccattacgg gcgggctgat tctgtcctcc
     2881 caatccgcac tggtcgtcaa caacaaaaca gggcaaatac tgtatcagaa aaacgccgac
     2941 aggattatgc ccattgcctc catttccaag ctcatgagcg cgatggtcgt tttggatgca
     3001 aacttggaca tgaacgaaac cgttaccatc acacccgacg aaatcgaccg cctcaaaggg
     3061 accggcagcc gtcttgccat aggtacggca cttacacgta aagaactgct gcaccttagc
     3121 ctgatgagca gcgaaaaccg cgccacccat gcattgggca gaacctaccc cggcggcatg
     3181 ggcgcatttg tcgccgccat gaaccgcaaa gcccaaagcc tgggcatgta cggcagccgc
     3241 ttttacgaac cgaccggact caacttccaa aacgtttcca ccgccaaaga cctgagcctc
     3301 atggtcaacg ccgccgccca atatccgcaa atccgcacca actcgacttc caactacgcc
     3361 tcggtacaaa ccaaaaacgg gcagcaaaac tataaaaact ccaatgccct ggtcagggaa
     3421 ggcatgtgga acatcgaatt gcagaaaacc ggctacatcc gcgaggcagg caggtctatg
     3481 gttgtcaaag ccaacattca aaaccaaccc gttaccatcg tattgctgaa ctcgcccaca
     3541 tccgccacac gcgtcaacga cgcccgcaaa atcgaatcgt ggatgctgca gcaacgctcc
     3601 tgacatacaa atgcccggcg gaaaaccgcc caaacacaaa aaaattggta atttttccta
     3661 ttccaggcta caattcgaca cgcacagggc atctgcccga tttcacaatc ataatagcgg
     3721 agttaaaaaa tatgacagct aaaggacaaa tgttgcaaga tcccttcctg aacgcattgc
     3781 gtaaagagca tgttccggtt tcgatttact tagttaacgg tatcaaattg caaggtcagg
     3841 tcgaatcttt cgaccaatac gtcgttcttc tgagaaacac ttccgtaacc caaatggttt
     3901 acaaacacgc catttccacc atcgtaccgg ctcgctccgt caacctccaa catgaaaaca
     3961 aaccccaagc cgcaccggct tcgacccttg tccaagtgga aaccgtccag cagcctgccg
     4021 aataatccgc acgaagcatg acgtgtcata tctttcaata ccttaccgga caacggtaag
     4081 gtatttttat tttcaggcgg catttaaaaa tattattgaa aaacatcctt ccattcgccc
     4141 attgcctttt gcgggaggca cttcccgaag gcggcaatgc gctggacggc accgccggca
     4201 acggacacga cacccttttc ctcgcacaaa ccgcaggcaa cagggggaaa gtgtgggcat
     4261 tcgacatcca gccgcaagcc ctgaacaaca cccgatgccg tctgcaggaa gcaggttaca
     4321 gcaatgtacg gctcatcttg gacggacatg aaaacctgaa gcaatatatt ccggagccgc
     4381 tggatgcagc cgttttcaat ttcggctggt tgcccggagg cgacaagaat ctgaccaccg
     4441 acaaggaaac cagcattccc gccctttgag caaccctctc cctgctgaaa gaagacggta
     4501 tgcttattgc cgtcctctat ccgggacacg aaaacggcaa acaggaggca gaagccgtcg
     4561 gacaatgggc aaaaaacctg cctcaagaac agtttgccgt tttacattac ggctttacca
     4621 accggaagaa tagcccaccc tatcttttgg catttgaaaa actgcgtcaa aaataactgt
     4681 ttgcggtaaa ataagccgat tctaaccctt attttgatac gagccgacca tgcagtatct
     4741 gattgtcaaa tacagccatc aaatcttcgt taccatcacc attttggtat tcaacatccg
     4801 ttttttccta ctttggaaaa atccagaaaa gcccttggtc ggcttttgga aagcactgcc
     4861 ccacctcaac gacacgatgc tgctgtttac gggattgtgg ctgatgaaga ttacccattt
     4921 ctccccgttc aacgcgcctt ggctcggcac aaaaatcctg ctcctgttcg cctacatcgc
     4981 actgggcatg gtaatgatgc gcgcccgtcc gcgttcgacc aagttctaca ccgtttacct
     5041 gctcgctatg tgttgcatcg cctgcatcgt ttaccttgcc aaaaccaaag tcctgccatt
     5101 ctgaaacact gccatgaaca acagacattt tgccgtcatc gccttgggca gcaaccttga
     5161 caaccccgca caacaaatac gcggcgcatt agacgcgctc tcgtcccatc ctgacatccg
     5221 gcttgaacag gcttcctcac tgtatatgac cgcacccgtc ggttacgaca atcagcccga
     5281 tttcatcaat gccgtctgca ccgtttccac caccttggac ggcattgccc tgcttgccga
     5341 actcaaccgt atcgaagccg atttcggacg cgaacgcagt ttccgcaatg caccgcgcac
     5401 attggatttg gacattatcg actttgacgg catctccagc gacgaccccc gccttaccct
     5461 gccgcatccg cgcgcgcacg aacgcagttt cgtcatacgc cctttggcag aaatcctccc
     5521 tgattttatt ttgggaaaat acggaaaggt tgtcgaattg tcaaaacggc tgggcaatca
     5581 aggcatccgt cttttaccgg acaggtaatt ccgcacgcgg atgccgtccg aacaccggtt
     5641 tcagacggca tttttccttt gccgccaaca cgcgtgcaaa aaaaatcgcc ccttgagaaa
     5701 ggggcgcaaa aggaacacaa accactacca aaactttaaa tctgcaacac tgcctgccgc
     5761 atactgtacc cgacaggata taaggccctc actaaatcgt ttcgagaaat ccaaacttct
     5821 tcatcaccga cagaaaatct gcctttctcc ggcaccagct ccaacaaaaa cggctgaacc
     5881 gccgtatgca gcttgtcctt acaccgcccc agctttctgg acaacgcgga cagggcgcgt
     5941 ttgtaggcat tatccttgcg gtcgagctcc ctcgcactac cgacccagct caaacgaagg
     6001 ttgcggcttt catcttcaat caacaaaata cctgtttcaa cttcccctac ctcgggttcg
     6061 cacgaaagcg caatataata tttgccgtca atattgacat atgccgcttc atgcacctga
     6121 cagttgcaac ccgaggttgc tagcagggaa taaacagaat cacggtgcag cctcaaaata
     6181 tcggaggaaa tctgtttgaa aatactttcc ggcatttttc cctcctttcg tctgctgcgt
     6241 tgtttactgg attgactgca ttatacagac atctcccaat tttacaaatg at
//
LOCUS       DAXFWM010000091         6074 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000091 DAXFWM010000000
VERSION     DAXFWM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.091"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            213..1753
                     /locus_tag="ACFI8B_001998"
     rRNA            213..1753
                     /locus_tag="ACFI8B_001998"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1854..1930
                     /locus_tag="ACFI8B_001999"
     tRNA            1854..1930
                     /locus_tag="ACFI8B_001999"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1888..1890,aa:Ile,seq:gat)
     gene            1936..2011
                     /locus_tag="ACFI8B_002000"
     tRNA            1936..2011
                     /locus_tag="ACFI8B_002000"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1969..1971,aa:Ala,seq:tgc)
     gene            2358..5249
                     /locus_tag="ACFI8B_002001"
     rRNA            2358..5249
                     /locus_tag="ACFI8B_002001"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5346..5459
                     /gene="rrf"
                     /locus_tag="ACFI8B_002002"
     rRNA            5346..5459
                     /gene="rrf"
                     /locus_tag="ACFI8B_002002"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5495..5614
                     /locus_tag="ACFI8B_002003"
                     /pseudo
     CDS             5495..5614
                     /locus_tag="ACFI8B_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
     gene            5716..>6074
                     /locus_tag="ACFI8B_002004"
     CDS             5716..>6074
                     /locus_tag="ACFI8B_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC3835132.1"
                     /translation="MSRGGATPYRFLLIRHIVARCGLLFATLKGKTMKKMFVLFCMLF
                     SCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPA
                     VLAKLKDQASVGAPAPKG"
BASE COUNT         1626 a         1320 c         1815 g         1313 t
ORIGIN      
        1 aagtatagca cggaatgtcg gaaatatcga gagatatctt agacaggcgg aagggatact
       61 ttataatttc gcaacgctct ttaacaaaac agattaccga taagtgtgag tgccttgggc
      121 ctcgcactgt ttgaaagaca gacgagatga tgttttgaac attgtcctgt cggtttcttt
      181 gaagcagacc agaagttaaa aagttagaga ttgaacataa gagtttgatc ctggctcaga
      241 ttgaacgctg gcggcatgct ttacacatgc aagtcggacg gcagcacagg gaagcttgct
      301 tctcgggtgg cgagtggcga acgggtgagt aacatatcgg aacgtaccgg gtagcggggg
      361 ataactgatc gaaagatcag ctaataccgc atacgtcttg agagggaaag caggggacct
      421 tcgggccttg cgctatccga gcggccgata tctgattagc tggttggcgg ggtaaaggcc
      481 caccaaggcg acgatcagta gcgggtctga gaggatgatc cgccacactg ggactgagac
      541 acggcccaga ctcctacggg aggcagcagt ggggaatttt ggacaatggg cgcaagcctg
      601 atccagccat gccgcgtgtc tgaagaaggc cttcgggttg taaaggactt ttgtcaggga
      661 agaaaaggcc gttgccaata tcggcggccg atgacggtac ctgaagaata agcaccggct
      721 aactacgtgc cagcagccgc ggtaatacgt agggtgcgag cgttaatcgg aattactggg
      781 cgtaaagcgg gcgcagacgg ttacttaagc aggatgtgaa atccccgggc tcaacccggg
      841 aactgcgttc tgaactgggt gactcgagtg tgtcagaggg aggtggaatt ccacgtgtag
      901 cagtgaaatg cgtagagatg tggaggaata ccgatggcga aggcagcctc ctgggataac
      961 actgacgttc atgtccgaaa gcgtgggtag caaacaggat tagataccct ggtagtccac
     1021 gccctaaacg atgtcaatta gctgttgggc aacttgattg cttggtagcg tagctaacgc
     1081 gtgaaattga ccgcctgggg agtacggtcg caagattaaa actcaaagga attgacgggg
     1141 acccgcacaa gcggtggatg atgtggatta attcgatgca acgcgaagaa ccttacctgg
     1201 ttttgacatg tgcggaatcc tccggagacg gaggagtgcc ttcgggagcc gtaacacagg
     1261 tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg
     1321 caacccttgt cattagttgc catcattcgg ttgggcactc taatgagact gccggtgaca
     1381 agccggagga aggtggggat gacgtcaagt cctcatggcc cttatgacca gggcttcaca
     1441 cgtcatacaa tggtcggtac agagggtagc caagccgcga ggcggagcca atctcacaaa
     1501 accgatcgta gtccggattg cactctgcaa ctcgagtgca tgaagtcgga atcgctagta
     1561 atcgcaggtc agcatactgc ggtgaatacg ttcccgggtc ttgtacacac cgcccgtcac
     1621 accatgggag tgggggatac cagaagtagg tagggtaacc gcaaggagtc cgcttaccac
     1681 ggtatgcttc atgactgggg tgaagtcgta acaaggtagc cgtaggggaa cctgcggctg
     1741 gatcacctcc tttctagaga aagaaggggc tttaggcatt cacacttatc ggtaaactga
     1801 aaagatgcgg aagaagcttg agtgaaggca aggttcgctt aagaagggaa accgggtttg
     1861 tagctcagct ggttagagca cacgcttgat aagcgtgggg tcggaggttc aagtcctccc
     1921 agacccacca agaacggggg catagctcag ttggtagagc acctgctttg caagcagggg
     1981 gtcatcggtt cgatcccgtt tgcctccacc aaaactttac aaatgaaagc aagtttgctg
     2041 tttttagcag cttattttga tttgcgaagt agaataacga cgcatcgatc tttaacaaat
     2101 tggaaagccg aaatcaacaa acaaagacaa tgagtttgtt ttgatttttt attctttgca
     2161 aaggataaaa acctctcgca agagaaaaga aaacaaacat agtatttggg tgatgattgt
     2221 atcgacttaa tcctgaaaca caaaaggcag gattaagaca caacaaagca gtaagcttta
     2281 tcaaagtagg gatttcaagt ttgcttactt agtcaacggg taggtaaacg aagtcaaaga
     2341 agttcttgaa atgatagagt caagtgaata agtgcatcag gcggatgcct tggcgatgat
     2401 aggcgacgaa ggacgtgtaa gcctgcgaaa agcgcggggg agctggcaat aaagctatga
     2461 ttccgcgatg tccgaatggg gaaacccact gcattctgtg cagtatccta agttgaatac
     2521 ataggcttag agaagcgaac ccggagaact gaaccatcta agtacccgga ggaaaagaaa
     2581 tcaaccgaga ttccgcaagt agtggcgagc gaacgcggag gagcctgtac gtaataactg
     2641 tcgagataga agaacaagct gggaagcttg accatagcgg gtgacagtcc cgtattcgaa
     2701 atctcaacag cggtactaag cgtacgaaaa gtagggcggg acacgtgaaa tcctgtctga
     2761 atatgggggg accatcctcc aaggctaaat actcatcatc gaccgatagt gaaccagtac
     2821 cgtgagggaa aggcgaaaag aaccccggga ggggagtgaa acagaacctg aaacctgatg
     2881 catacaaaca gtgggagcgc cctagtggtg tgactgcgta ccttttgtat aatgggtcaa
     2941 cgacttacat tcagtagcga gcttaaccgg ataggggagg cgtagggaaa ccgagtctta
     3001 atagggcgat gagttgctgg gtgtagaccc gaaaccgagt gatctatcca tggtcaggtt
     3061 gaaggtgccg taacaggtac tggaggaccg aacccacgca tgttgcaaaa tgcggggatg
     3121 agctgtgggt aggggtgaaa ggctaaacaa actcggagat agctggttct ccccgaaaac
     3181 tatttaggta gtgcctcgag caagacactg atgggggtaa agcactgtta tggctagggg
     3241 gttattgcaa cttaccaacc catggcaaac tcagaatacc atcaagtggt tcctcgggag
     3301 acagacagcg ggtgctaacg tccgttgtca agagggaaac aacccagacc gccggctaag
     3361 gtcccaaatg atagattaag tggtaaacga agtgggaagg cacagacagc caggatgttg
     3421 gcttagaagc agccatcatt taaagaaagc gtaatagctc actggtcgag tcgtcctgcg
     3481 cggaagatgt aacggggctc aaatctataa ccgaagctgc ggatgccggt ttaccggcat
     3541 ggtaggggag cgttctgtag gctgatgaag gtgcattgta aagtgtgctg gaggtatcag
     3601 aagtgcgaat gttgacatga gtagcgataa agcgggtgaa aagcccgctc gccgaaagcc
     3661 caaggtttcc tacgcaacgt tcatcggcgt agggtaagtc ggcccctaag gcgaggcaga
     3721 aatgcgtagt cgatgggaaa caggttaata ttcctgtact tgattcaaat gcgatgtggg
     3781 gacggagaag gttaggttgg caagctgttg gaatagcttg tttaagccgg taggtggaag
     3841 acttaggcaa atccgggttt tcttaacacc gagaagtgat gacgagtgtc tacggacacg
     3901 aagcaaccga taccacgctt ccaggaaaag ccactaagct tcagtttgaa tcgaaccgta
     3961 ccgcaaaccg acacaggtgg gcaggatgag aattctaagg cgcttgagag aactcgggag
     4021 aaggaactcg gcaaattgat accgtaactt cgggagaagg tatgccctct aaggttaagg
     4081 acttgctccg taagccccgg agggtcgcag agaataggtg gctgcgactg tttattaaaa
     4141 acacagcact ctgccaacac gaaagtggac gtatagggtg tgacgcctgc ccggtgccgg
     4201 aaggttaatt gaagatgtgc aagcatcgga tcgaagcccc ggtaaacggc ggccgtaact
     4261 ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc acgaatggcg
     4321 taacgatggc cacactgtct cctcccgaga ctcagcgaag ttgaagtggt tgtgaagatg
     4381 caatctaccc gctgctagac ggaaagaccc cgtgaacctt tactgtagct ttgcattgga
     4441 ctttgaagtc acttgtgtag gataggtggg aggcttggaa gcagagacgc cagtctctgt
     4501 ggagtcgtcc ttgaaatacc accctggtgt ctttgaggtt ctaacccaga cccgtcatcc
     4561 gggtcgggga ccgtgcatgg taggcagttt gactggggcg gtctcctccc aaagcgtaac
     4621 ggaggagttc gaaggttacc taggtccggt cggaaatcgg actgatagtg caatggcaaa
     4681 aggtagctta actgcgagac cgacaagtcg ggcaggtgcg aaagcaggac atagtgatcc
     4741 ggtggttctg tatggaaggg ccatcgctca acggataaaa ggtactccgg ggataacagg
     4801 ctgattccgc ccaagagttc atatcgacgg cggagtttgg cacctcgatg tcggctcatc
     4861 acatcctggg gctgtagtcg gtcccaaggg tatggctgtt cgccatttaa agtggtacgt
     4921 gagctgggtt taaaacgtcg tgagacagtt tggtccctat ctgcagtggg cgttggaagt
     4981 ttgacggggg ctgctcctag tacgagagga ccggagtgga cgaacctctg gtgtaccggt
     5041 tgtaacgcca gttgcatagc cgggtagcta agttcggaag agataagcgc tgaaagcatc
     5101 taagcgcgaa actcgcctga agatgagact tcccttgcgg tttaaccgca ctaaagggtc
     5161 gttcgagacc aggacgttga taggtggggt gtggaagcgc ggtaacgcgt gaagctaacc
     5221 catactaatt gcccgtgagg cttgactcta tcatttgaag gacttcaaga gataaaagct
     5281 tactgactga ttcagtcatt accgaatata ttgattaagg ctttaccgat ttgcaacagt
     5341 ttaagtttgg cggccatagc gagttggtcc cacgccttcc catcccgaac aggaccgtga
     5401 aacgactcag cgccgatgat agtgtggttc ttccatgcga aagtaggtca ctgccaaaca
     5461 ccctttcaga aaacccccgg atatccgggg gtttttgctt tgcccggaaa aaatgtcggg
     5521 gatggcggga cggcatctgt acggtgtccg gtcgggtttg cggaggaacg gcttgaaact
     5581 ttgggatatt cattttagaa tgacccgttt tatagcggat taacaaaaat caggacaagg
     5641 cggcgggccg caggcagtac ggatagtacg gaaccggttc gcccggtgct tcatcacctt
     5701 agggaatcgt tccctttgag ccggggcggg gcaacgccgt accggttttt gttaatccgc
     5761 catatcgtcg caagatgcgg tttgttgttt gcaaccctta aaggaaaaac catgaaaaaa
     5821 atgtttgtat tgttctgtat gctgttctcc tgcgccttct cccttgcggc ggtaaacatc
     5881 aatgcggctt cgcagcagga gctggaggcg ctgccgggca taggcccggc gaaggcgaag
     5941 gccattgcgg aataccgcgc gcaaaacggc gcgttcaagt ctgtggacga tttgatcaag
     6001 gtgaagggca tcggtccggc ggtgctggcg aagctgaaag accaggcttc cgtcggcgcg
     6061 cccgcaccaa aagg
//
LOCUS       DAXFWM010000092         6002 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000092 DAXFWM010000000
VERSION     DAXFWM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.092"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            122..1957
                     /gene="edd"
                     /locus_tag="ACFI8B_002005"
     CDS             122..1957
                     /gene="edd"
                     /locus_tag="ACFI8B_002005"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC3835133.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            2167..2805
                     /locus_tag="ACFI8B_002006"
     CDS             2167..2805
                     /locus_tag="ACFI8B_002006"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC3835134.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(2994..3179)
                     /locus_tag="ACFI8B_002007"
     CDS             complement(2994..3179)
                     /locus_tag="ACFI8B_002007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835135.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(3190..3302)
                     /locus_tag="ACFI8B_002008"
                     /pseudo
     CDS             complement(3190..3302)
                     /locus_tag="ACFI8B_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3400..4439)
                     /locus_tag="ACFI8B_002009"
                     /pseudo
     CDS             complement(3400..4439)
                     /locus_tag="ACFI8B_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4602..5642
                     /gene="mutY"
                     /locus_tag="ACFI8B_002010"
     CDS             4602..5642
                     /gene="mutY"
                     /locus_tag="ACFI8B_002010"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC3835136.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
BASE COUNT         1534 a         1712 c         1435 g         1321 t
ORIGIN      
        1 aggcaacgcc ggaccggttt aaatttaatc cactatactc accgtctaac atacccgata
       61 caaaaatcag aaacgcacaa acaaatcccc aataccaccc cgttccgcca ggagaccgaa
      121 catgaaccac actcctatcc accccaaact cgccgaaatc accgggcgca ttatcgaacg
      181 cagccgtccg acgcgtgaaa aatatctggc gaaaatccgc agtgccaaac agatgggacg
      241 cttagagcgc aaccagctcg gctgcagcaa tttggcgcac ggctatgccg ccatgcccaa
      301 aagcatcaaa atcgaaatgc ttcaggaaac cgtccccaac ttaggcatca tcactgccta
      361 caacgacatg gtttccgcac accagccgtt taaagacttc cctgaccaaa tcaaagacga
      421 agcacaaaaa aacggcgcga ccgcacaagt cgccggcggc acgcctgcca tgtgcgacgg
      481 catcacgcaa ggctacgccg gcatggaatt gtcactgttt tcccgcgacg tgattgcgat
      541 gagtaccgcc gtcgggctgt cgcaccaaat gtttgacggc agcctgttta tgggcgtgtg
      601 cgacaaaatc gttccgggtc tgatgatagg tgcgctttcg ttcggtcata ttcccggtat
      661 cttcgttccg gcaggcccga tgtccagcgg catcggcaat aaagaaaaag cccgtacccg
      721 ccaacttttc gccgaaggca aagtcggacg cgacgaactt ttgaaaagcg aaatgggttc
      781 ttaccacagc ccgggcacct gcactttcta cggcacggca aactccaacc aaatgatgat
      841 ggaaatgatg ggcgtacacc tgcctgccgc cgcctttgtc cacccctata ccgacctgcg
      901 cgaagccctg acccgctacg ccgccggaca cctcgcgcgc ggcatcaaaa acggcacgat
      961 taaacctttg ggcgaaatgt tgaccgaaaa atccttcatc aatgccctga tcggtttgat
     1021 ggcgaccggc ggctcgacca accacaccat gcacctcgtc gctatggcgc gcgccgcagg
     1081 cgtgattttg aactgggacg acttcgacga aatttcttcc atcatcccgc tgctcatccg
     1141 cgtgtatccc aacggcaaag ccgacgtgaa ccactttacc gcagcaggcg gcctgccttt
     1201 cgttatccgc gaattgctgg acgcaggctt attgcacgac gatgtcgata ccgtcgtcgg
     1261 acacggtatg cgccactaca ctaaagagcc tttcctcatc gacggcaaac tcgaatggcg
     1321 cgaagccccc gagaccagcg gcaacgacga catcctgcgt aaagccgaca atccgttctc
     1381 cccggacggc ggtctgcgcc tgatgaaagg caacatcgga cgcggcgtgg ttaaagtgtc
     1441 agccgtacgc gaaggctgcc gcatcatcga agcacccgcc atcgtgttca acgaccagcg
     1501 cgaagtgttg gcggcatttg aacgcggcga gttggaacgc gattttatct gcgtcgtccg
     1561 ctaccaaggc ccgcgtgcca acggtatgcc cgaattgcac aaactgaccc cgcctttggg
     1621 catcctgcaa gaccgcggct tcaaagtggc gctgctgacc gacggccgta tgtccggcgc
     1681 gtcaggcaaa gtgcccgcgt ccatccacat gacgcccgaa gccctgatgg gcggcaacat
     1741 cgccaaaatc cgtaccggcg acctgattcg cttcgactcc gttaccggcg aactcaacgt
     1801 cctgattaac gaggccgaat ggaacgtccg agaagtcgag cgcatcgatt tgggcgcgaa
     1861 ccaacaaggc tgcggccgcg aactcttcgc cggcttccgc agtatgacca gcagtgcgga
     1921 aaccggtgcg atgagcttcg gcggagaatt tgcctgatgc gcgtttcaga cggcctttta
     1981 aaacagaagg ccgtctgaaa aattattgaa gccgtttaaa atagatgcag acgtagcgtg
     2041 agctttgccc gcgaacaaaa cccaagcaac atcagaatgt tttaccccaa aggttgtctg
     2101 aaaacttaac cccccccatc ccgtttgacc tcaacaaaaa cgacaccccc cacttggaga
     2161 accgaaatgt ccaaactgac cccccgcgaa atcctgaccg ccggcgcagt tgtgcctgtg
     2221 atggcgattg acgacttaag caccgccgtc gatttgtccc gcgcccttgt cgaaggcggc
     2281 atccccaccc tcgaaattac cttgcgcacc cccgtcggac tcgaggccat ccgcctgatt
     2341 gccaaagaaa tgcccaacgc catcatcggc gcaggtacgg taaccaatcc cgaacagctc
     2401 aaagccgtcg aagacgcagg cgcggttttc gccatcagcc cgggtttgca cgaatccctc
     2461 gccagagccg gccgcaacag cggcattccc ctgattcccg gcgttgccac tccgggcgaa
     2521 gtccaactgg ctttggaaca cggcatcgac acactcaaac tcttccctgc cgaagtcgtt
     2581 ggcggcaaag cgatgctcaa agccctctac ggaccttacg ccgacgtacg cttctgcccg
     2641 accggcggca tcagcctcgc cactgcgccc gattacttgg cattgcccaa cgtcttatgc
     2701 gtcggcggct cttggctgac accgaaagaa gccgtgaaaa acaaagactg ggacaccatc
     2761 acccgcctcg ccaaagaagc ggcggcattg aaacccaaag cctgaaccgc atcgtaaaaa
     2821 tgccgtctga agccctttcc agtctcagac ggcattttgc cgcttggcga ctcagtcggc
     2881 atacacagca gcaccgcccc taaaatgccc atccatctcc agaacaatgc gaatgacgcg
     2941 cgatatgcgg agacctatga cgaaaaaata attataatat atttatcaat tagtcataaa
     3001 aatcgcccta tcggtaaaat gccgtgatga gcggctgaaa ctaacaagtt aaccgtcgtt
     3061 aaaaaataac gctcagagac ctttgcaaaa ttccttttct tcaaacagcc gacatccaac
     3121 cacaggtttt tcggctgttt tcgtttcaaa taaccgccgt tcccaccaaa atatcccatt
     3181 aatccccctt aaatatagtg gattaaattt aaaccagtac ggcgttacct cgccttgccg
     3241 tactatttta ctgtctgcgg cttcgttgcc ttgtcctgat ttaaatttaa tccactatac
     3301 ctgattgatc aggaacagcc cgttttacac ggctgaaaaa atgccgtctg aaacacgttc
     3361 agacggcatc cgcttaaaaa acaactgatt caacggcgat taatccgctt ccaaaaccac
     3421 tttcatcgct tggttttcgg cggcgtgttt gaacacgtcg taggcttttc caattggctg
     3481 aatttgaaac ggtgggtcaa cattttggtg taatccactg agctgctgaa aattgccttc
     3541 atcagtattt cggtggtgtt ggcattgacc aaaccggtag tgatggcaag atttttaatc
     3601 cacagttttt ccagtttgaa atcaacggat tgaccgtgta cgccgacgac ggcgatatga
     3661 ccgccgggtt tcacgatgtc ttggcacata ttccaagttg cagggatacc gaccgcttcg
     3721 atggcacaat ctacgccgtc ttcgccgacg atggcgaaaa cttgtttgga gacgtcgccg
     3781 gaagcggggc tgatggtatg ggtcgcgccc aactctttcg ccagtttcaa acggttttcg
     3841 tccatatcgc acacgataat ggcggcaggg ctatacagtt gggcggtcaa caaggcggac
     3901 ataccgacag ggccggcacc tgcgatgaat acggtatcgc cgggtttcac atcgccgtat
     3961 tgcacgccga tttcgtgggc ggtcggtaaa gcgtcgctca acagcagggc gatttcttcg
     4021 ttgacgttgt cgtgcggcgg cacgaggctg ttgtcggcat aaggcgtgcg gacgtattcg
     4081 gcctgcgtgc cgtcaatcat atagcccaaa atccagccgc cgttgcggca gtgcgaataa
     4141 agttggattt tgcagttgtc gcaagtgcag catttgctga cgcatgaaat aatgacttta
     4201 tcgccgactt tgatgttttt tacagcctcg ccgacttctt ctacaatacc gatgccttca
     4261 tgaccgagga tacgaccgtc ggcgacttcg gggtttttgc ctttccaaat acccaagtcg
     4321 gtaccgcaaa tcgtggtttt gacgattttc accaccgcat cggtcggatc gataatctgc
     4381 gggcggggtt tttcttcaaa acggatgtcg tttgcgccgt gataaaccat tgctttcatg
     4441 ctgatactcc ttgcttgttg ataaataatt tcaataccgc aacaaagttt ctttatatga
     4501 gttatatgcc cctacaaaaa ataagtcaat aagaattatt ttcacaattt tatacaacaa
     4561 catactgttt taaatataaa taaaaccacc gattgatatt aatgaacaca cccatcccct
     4621 tctccgaacg gctcatccgc tggcaaaaac aacacggtcg ccaccacctc ccttggcagg
     4681 tcaaaaaccc ttattgcgtc tggctttccg aaatcatgct ccagcaaacg caggtcgccg
     4741 ccgtgttgga ctactatccg cgtttcttgg aaaaattccc gaccgttcag acgcttgccg
     4801 ccgcgccgca agacgaagtg ttgtcgttgt gggcgggctt gggctattac ggccgcgcgc
     4861 gcaatctgca caaagccgcg caacaaatcg tcggacaatt cggcggtacg tttccatcgg
     4921 agcgcaaaga cttggaaacg ctctgcggcg taggcagaag caccgccgcc gccatttctg
     4981 cctttgcttt caaccgacga gaaaccatct tggacggcaa cgtcaaacgc gttctctgcc
     5041 gtgtttttgc ccaagacggc aatccgcaag ataaaaaatt tgaaaactcg ctctggacac
     5101 ttgccgaaag cctgatgccg tctgaaaacg ccgatatgcc tacttacacg caaggtttga
     5161 tggatttggg cgcgaccgtg tgcaaacgga cgaaaccctt gtgccgtcaa tgccctatgg
     5221 cggacatctg cgaagcaaaa aagcaaaacc gcaccgccga gctgccgcgt aaaaaaactg
     5281 ccctcgaagt gcaaaccctg ccgctttact ggctgattgt ccgcaaccgg gacggcgcaa
     5341 tcttgctgga aaaacgcccc gccaaaggca tttggggcgg actgtattgc gtgccgtgtt
     5401 ttgaaagttt gaacgggctt tccgactttg ccgccaaact ctccctgacg atggcggata
     5461 tggacgaaca aaccgccctg acccaccgcc tgacgcaccg gctgctgatg attacgccgt
     5521 ttgaagggca aatgccgtcc gaacaccatt cagacggcat ttggataaag ccgggccatt
     5581 tgaaagatta cggcctgccc aagcctttgg aaatttattt aaacggtaat aggttagaat
     5641 aaacaaaata aacccgttga actgttgtta gcaggtatcg cagcaagaac aaccgatgaa
     5701 tttgggtcgt attttaggca gcgggataat gttcaaatgg gacatttgga acggaaaaag
     5761 tcggcaattt aaaaaggatt ttagaaacaa agagggtcaa aaacatgaac acaaacttaa
     5821 atgacaaaga caaagccatg gataccgcaa tcaggtttca gaaaaggatg aggattccga
     5881 aatttttctt tttaattctc ggaatcacag tggttttggc atttatccaa gacgtgataa
     5941 cgggttctaa ttttctgcaa ataacaatta tgtaaataat atttgtgata tatttcagtt
     6001 tc
//
LOCUS       DAXFWM010000093         5549 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000093 DAXFWM010000000
VERSION     DAXFWM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.093"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(212..592)
                     /locus_tag="ACFI8B_002011"
     CDS             complement(212..592)
                     /locus_tag="ACFI8B_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC3835137.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
     gene            complement(1083..1427)
                     /locus_tag="ACFI8B_002012"
     CDS             complement(1083..1427)
                     /locus_tag="ACFI8B_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835138.1"
                     /translation="MNSLPLKNPKLIAVSVCAVFVAGAWQYDRAAQYRRGYGAAMLEV
                     SERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADG
                     VRELNAAVDDGG"
     gene            complement(1408..1569)
                     /locus_tag="ACFI8B_002013"
     CDS             complement(1408..1569)
                     /locus_tag="ACFI8B_002013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835139.1"
                     /translation="MMNPTEFLKARIAEWEAKSKEAGGNADFKAFEFAESEIKNYKAM
                     SEAYEQPAA"
     gene            complement(1569..2105)
                     /locus_tag="ACFI8B_002014"
     CDS             complement(1569..2105)
                     /locus_tag="ACFI8B_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC3835140.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLTAAATGAQGAGEA"
     gene            complement(2408..3352)
                     /locus_tag="ACFI8B_002015"
     CDS             complement(2408..3352)
                     /locus_tag="ACFI8B_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC3835141.1"
                     /translation="MNIIGPDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKINDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKPAYYIVKTGQPYDAERH"
     gene            complement(3567..4754)
                     /locus_tag="ACFI8B_002016"
     CDS             complement(3567..4754)
                     /locus_tag="ACFI8B_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC3835142.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            complement(4755..5039)
                     /locus_tag="ACFI8B_002017"
     CDS             complement(4755..5039)
                     /locus_tag="ACFI8B_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC3835143.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            5266..5549
                     /locus_tag="ACFI8B_002018"
                     /pseudo
     CDS             5266..5549
                     /locus_tag="ACFI8B_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
BASE COUNT         1195 a         1383 c         1305 g         1666 t
ORIGIN      
        1 cggcaaggta aacggaaaat gccgcctgaa ggttcggacg gcatttttat tgaagggatg
       61 attggggatt tgtaggcgtt tgcagatacg acaaggggcg cattccgcgc cccatcacac
      121 gcacggacgg cttgtcatcg cctgtcgccc gtgcgcggct gcccatattt cggaacagtc
      181 ttgcaagccc ctttaaggga acggttttat tctagtacag tttgaatgcc ttggcaacgg
      241 ctgttttaac cgcctgaaaa tcctcttcac tgattattgg gatgcaacgg tcgcgccctt
      301 tgggtttgta tcggtctaat cgtgccaatc cgactgttgc cgtcatatcg cattttgccc
      361 gacattggat gtgcggcttg tccggtaagg ggttttcact cattttgtgg tggcagtccg
      421 ccaaagggac aggctctgtg ctgcttaagg gtacgaccgt taccagtttg ccgttgtgcc
      481 tgtttcgcgc tatgacgacg acagggcgtt tcttgaccat ttccggttct tcataaccgc
      541 gaaagtcgca catgataact gaacgttccc ttggttggaa ttttaaaggc attagccgct
      601 cctgacgatg acaggcggct gttatggcag ttcttacaca aaaaaccgct ttgtgtaagg
      661 cggttgcaaa aaaaagcctt ccaataaaaa tgccgcctga acctttcaga gtgcgaagcc
      721 tgcccatagg gtacggcatt ttcatttccg cccgattatt ggtgcgcctg ataattttgt
      781 ataacgcccc cttcgtgcaa acaaaacggc aaaaatttgc cgcgtccgcc cgatatgtaa
      841 aaaatcgggg ggaatgccga cacttccccc gatgcggccg ccaacccgga accgacggtt
      901 cggttgcaaa ctttcggttg gcaactcaat ccgcgcccaa cgcccgtacc agcgcgccgt
      961 gccgcgccct gcagtcgtta tacataccgg ccgccttcag ggcccacggc agcacgtccg
     1021 cgcccgtgtt cccttcaagg tgcggcagtt tcgggcaggg ccgcaccaaa tcggcgggcg
     1081 gcttaaccgc cgtcgtcaac ggcggcgttg agttcgcgca cgccgtcagc atcaaaacag
     1141 gcattgcggt acacaggttt ttcaatgatt ttaagcgttt gcacatagcg caccctttct
     1201 ttttcctcac gcgccgcctt ttgcgcctga tacgcggcgg acgatttgcg ggcgtgttcg
     1261 gcgtgttcga ccgcggcggc tttgaggcgt tccgaaacct ccagcatagc cgcaccgtat
     1321 ccgcgccggt attgcgcggc gcggtcgtat tgccacgcgc cggcaacgaa cacggcgcaa
     1381 accgacaccg caatcagctt cgggttttta agcggcaggc tgttcatagg cctccgacat
     1441 cgctttgtag tttttgattt cgctttcggc aaactcaaaa gctttaaaat cggcgtttcc
     1501 gcccgcctct ttgcttttgg cttcccattc ggcgatacgc gctttcagaa actcggtagg
     1561 attcatcatt acgcctctcc cgcgccttgc gcgccggtgg cggcggcagt caaccggtaa
     1621 cgcccttcgg cgggggacga aggcacaggt ttgccgccaa tcagcttgga cggccagaag
     1681 tagccgtcaa tgtccgcagg gtcaaacggg atgatggata ccatattgcc ctgattgccg
     1741 cccaagccca agattctgcc ttccgcgtct ttgccgacaa cgaagaacac gtgtccgccg
     1801 ccccggcgcg gtttgaccgc gatgcagccg tatgcggggg cttcgagttt cgtcaaaccc
     1861 gacattgacc aagccttggc gcgataccag tccctgatga ccgcgcgtcc cgctttgccc
     1921 aggcagtgtc cgacaaacag gccgcaccac ggcgtttcgt cttcaaaata ccaagacttt
     1981 gccgcgccgg ggaagccgcc cgtctctttg agccattgca caatcgtcgg attgtgtttc
     2041 gcgccgggaa tttctttcaa accgatgtgc cttctcgctt cggctatcca cggtaattct
     2101 gtcattttta atcctttcgg taaaagacga gatgtaaact ttgggttggc aactcgttct
     2161 atgttttaag tcgcaatcac aatgtgcacc gcatcgccga acacgctgtc tttttcagtc
     2221 gcgtttgccg atacggtaac tttccgcgca gatgatatgt tgcccgtcaa cacaaaataa
     2281 aaaacaaagt tgcaacatgc tgatttatat tgttattttt atttacgttt atttacgata
     2341 tgcaaatgca cggttacaaa gatatatttg cataactgtt taattttatt gaattttatt
     2401 gattcaatca gtgtctttcc gcatcgtaag gctgcccggt tttaacaatg taataggcgg
     2461 gcttcgccag tttgcgcatg atggcaacga taattaccat ctttggctta cccgcttttt
     2521 tcagattatt tattaatttc ggaaatgcgt taaaacggta agcacaaagg gcgggcatat
     2581 acagcgtact ttttaatcgt ctgtttccgt atcggctcaa tctgccccga cctcttacgc
     2641 ttgtccctga ttgtatgatg gcgggactta atccggcata ggatacaaat tggtttgcgg
     2701 ttttaaaatg tttttctgtc agttgcgcat aaagaactga tgcggtgtct ttgcctatgc
     2761 tcgggatggt ttgaagattg cggtaatggt tattgtccgt ttgttttttg atttgttcgg
     2821 atatggctat ttttacctgt tccatcttgt cctgtatggt atctatcaag tcttgatgta
     2881 tgttccttat gaagtcttct tcagtgctat gaagacggtt tttaatttgc ttctgctgtt
     2941 gctgtaattg gtttttaaga ttaatcagtt tttgcagtgc tttgtttttg ggtatctgat
     3001 acggtatcaa tgtatcttga tgccttttta tgtaatctgc tatcaggttt gaatctgctt
     3061 tgtcggtttt ggtacggtta aacctgcttt ttccgtagtc attgattttt aagggattga
     3121 taacgtaaac ggtatagtag gaagaaagca tatctgctgc cttttcgtaa tagatgcctg
     3181 ttgcctccat gccgatatag acttttctga ttctgtttcc ctttatccac aatctaaact
     3241 gttttaatcc atcatcatta ttcttaaatt taatgtaatg gatacttccg tttgttttat
     3301 gcaatgttgc gtctatggtg tcctttgaga tgtccggccc gattatattc attggtattt
     3361 tccttattta tacagccttg atacggctag gatgatattc aattttgagg atggataaag
     3421 gcagccggca tttctacgcg tctgttttaa tacattgcgg gatttgctgc ctgactgcct
     3481 tagcccttgc tttgcgcgaa acaaagaccc gtaagccgtc tatattcaaa cggtttacgg
     3541 gtcttttttc tctgttgccg ttttcttcag tttgccgatc cgaccacgcc cccgccgatt
     3601 ccttcaaacg gtttcccgcg ttcttcccaa ttgtcgtaca ttaggttctg ctgcggtttt
     3661 ccgcccaagg tggcaacttg cgccctgtcc gaatgttgct gcgcgctttg ctgaacttcc
     3721 tgcccttggc tttcttcttt gtatgggtta aacggcaagc cgttttttac atagtccttg
     3781 cacatcaact ccgtcacttc tttcaatgcc gtcccttgat gcgaatagca ggtgcatccg
     3841 gttcttccgc cttctataca gcctgctata tattcaaagg tccttacctg ccttacaccg
     3901 ttataaatcg gcttgctttc gggtttttcg ggcaatgtcg gaacaaacat atctgccgta
     3961 aggtttccgt tattcaccga ttctccttct gttttatccg gaagtactgc ctgctgttct
     4021 gttgccgccg attcttgtgc tgcgggttct tcctgttttt ttccgtaact gcccaacatt
     4081 ttgtaagaca aaccgacaaa tagcggaatc aataatatga tgacgggcaa tgcataaaac
     4141 cattttgaac gcttgacttt gtttaccgtg tgaatttctg cggattcgta caagtcataa
     4201 acttttttat ccagtgtgta gatactggaa aatgcacttg atgccatttt taccgggtca
     4261 tccgcgcata ctttccattc aagcagggta cgcaaaccca ttttgttggc cgcaatgtgg
     4321 taatgtcttt taaccaatgt tcgcaagttc tgatctaaga gtttaggacc ttgtgtcaat
     4381 acaaatatat ctatgccctg atgcctgtgt gtgttcagcc attggacgtt ttcggggatt
     4441 ttcgaacctg cggagcgtgc gggccatacg tcttgcgcct catcgacaat aacgattgcg
     4501 ccgacgtttt caggcttctt gatccattca tacatatcat gcgccgaaag ctgttcatcg
     4561 gttgatttcg gcagcttctt tgcgtctgtt tctatgtggg tgtgcggtat cttcaaacct
     4621 ttgatgttcg taaatacttt acggcgtacg ccgttttcat ctggcttaaa catttcatcg
     4681 tttgccatca tggaaaccat ttttaatgtt ttccctgaac cgggcgtgcc ggttatcaaa
     4741 cagatttctg ccatttattt tttcttcccg attgaggttg cgagttttgt catttgttta
     4801 aaggacagaa taaaggcgat tgcgccgaaa agaatgttta gaacagtacc accgccgctt
     4861 atatagaaaa gctgtaacat tgcttgaggc gcgcccgtta tgctatgggt tatcgcctgt
     4921 tgaaaatggg ctgccaatct atctacaccc gcataggtaa cagccatcaa gcctaatgca
     4981 gtcaatatac ggcctgcgac gctcatcaag agcggaatca atgcggccaa caatttcatt
     5041 tgctctccct ttcttaaaag gcacgtttgc ctcattaaac aaatgtttct taatctgaaa
     5101 gattgctccg cattcgcggc gcggcagcgc tgtggggaca ccccctcgcg cttatcgcca
     5161 atcccccccc cgcgcttctc ggctgtttgc ggcttcgccc gcaaagtgcg gtacttcgcc
     5221 tgccttggca caaggtaccg aagcccggcg gtacttcgcc tgcctttggc taaagttgct
     5281 taattggatt ggaataattt tgttttaaat cgttgcacat ttgattggaa cggcggaggt
     5341 tgtgcggtca ataaaggtga tgatttcaga ggtggggctt ctttttccct tggccgcaat
     5401 ccgaaataca aagaagaaat ggatgccaaa aagccggaag agattttatc gttgaaagtc
     5461 gatgccgatc ccgacaaata cataaaggca accggatatc ccggttattc cgaaaaagta
     5521 gaagtcgcac ccggaacaaa agtgaatat
//
LOCUS       DAXFWM010000094         5454 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000094 DAXFWM010000000
VERSION     DAXFWM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.094"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(34..771)
                     /gene="ubiE"
                     /locus_tag="ACFI8B_002019"
     CDS             complement(34..771)
                     /gene="ubiE"
                     /locus_tag="ACFI8B_002019"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC3835144.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(814..1182)
                     /locus_tag="ACFI8B_002020"
     CDS             complement(814..1182)
                     /locus_tag="ACFI8B_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC3835145.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            1302..2921
                     /gene="mnmC"
                     /locus_tag="ACFI8B_002021"
     CDS             1302..2921
                     /gene="mnmC"
                     /locus_tag="ACFI8B_002021"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC3835146.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            complement(3380..3454)
                     /locus_tag="ACFI8B_002022"
     tRNA            complement(3380..3454)
                     /locus_tag="ACFI8B_002022"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3419..3421),aa:Glu,seq:ttc)
     gene            complement(3472..3548)
                     /locus_tag="ACFI8B_002023"
     tRNA            complement(3472..3548)
                     /locus_tag="ACFI8B_002023"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3512..3514),aa:Arg,seq:acg)
     gene            complement(3659..5332)
                     /gene="recN"
                     /locus_tag="ACFI8B_002024"
     CDS             complement(3659..5332)
                     /gene="recN"
                     /locus_tag="ACFI8B_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC3835147.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
BASE COUNT         1350 a         1647 c         1186 g         1271 t
ORIGIN      
        1 gttcagacgg cattggcttc acaggcagtc cgtttaaaat ttcacgccct tatgcagcgc
       61 gacgatgccc gcactcatat tgtgataatc cacgctgtcg aagcctgcat ccagcatcat
      121 ctgtttcaaa gtttcctgat cggggtgcat acggatggat tcggcgagat actggtagct
      181 gtccgcatct ttggcaatca gcctgcccat caccggcaac agcttgaaag aataaaaatc
      241 gtatgcgcct tcgagcggct tgtagatttt ggaaaactcc aacaccagca acgtaccgcc
      301 cggtttcaaa acacggtaca tttccttaag cgcggcatct ttatacgtca tgttccgcaa
      361 gccgaacgcc acggagacca agttgaaata attgtcgggg aaaggcagtt tttccgcatc
      421 ggcaagcgac acgggtaaaa tcatgccttc gttcaacaga cggtcgcgcc cgacggtcag
      481 catagaagaa ttaatatcgg tcagccaaac ctcgccttcc ttgccgaccc gtttcgccca
      541 accgcgcgac aaatcgcccg taccgcccgc aatgtccaac actttatctc cttttttcag
      601 gtgcgccgta tggatggtaa aatgcttcca tacgcggtgc agccctgccg acatcacatc
      661 attcataatg tcgtagtttt tggcgacgga gtgaaacact tccgccactt ttccggcttt
      721 ctcatcttcg ttgaccgtgc tgaacccgaa atgcgttttt tgtccgccca tattctaacc
      781 acctccctga taccgcccta cggcatccca aacttaacaa tctgattgct tttcaaaacg
      841 cgacgcgccc gccgccgcca atttgtccaa atattcctgc cacatcgcat cgtatccgta
      901 tgccagtctg tgcagatacg cccaatcgta aagaccgctg tcgtgcccgt ccgaaaaact
      961 gattttcagc gcatactgtc cgacaggctg caaatccgta atctgcacat ccgccttacc
     1021 ggtctgcaaa acatcctgtc ccacgccgtg tccgcgcact tccgcactcg gcgaatagac
     1081 gcgtaaaaat tcggcaggca gatttttagg ttcgtcccca taagtcaaaa ccaacacggc
     1141 acggtttttc aacaaacgga tttcaacagg aatattattc aaaaaacacc tcggttcggc
     1201 atacaatatc cggcaggcgt acgcgtgcct ttaaacggcg cgtattttaa cagctttatc
     1261 ccgataccga tattcaaacc cacacttcag acggcattga tatggacaat ctcgtatggg
     1321 acggcattcc cgacatccgc acactcgacc aaaccatccg caaacacgca cacccgctca
     1381 acctgattgt ctgcctcccc gataatcaga ttcccgattt tcaaaccgca caagatgctt
     1441 cggactcgga atgccgtctg aagcaccgtt tggatcaggc aacccagtgc ctccagttcg
     1501 acagcatcaa cctcatcgaa cacatcctgc ccgatgtccg cttctggctg gttccccctt
     1561 cacgcacccg ccgcctgcac gaacacttcc accacatttc ctggcagacc gaagccatcc
     1621 cgcaaaccga aagcaagtcc gacaaaccct ggtttgcact tccacaaaca tccgaacgga
     1681 aaaaaccgga acacgtcctc gtcatcggtg caggcattgc cggcgcatcg accgcccacg
     1741 ccttagcatc acacggcatt tccgttaccg tattggaagc ccgaaaagcc gctcaagccg
     1801 ccagcggcaa ccggcaaggg ctgctttacg ccaaaatctc gccgcacgac accggacaga
     1861 ccgaactgct gcttgccggc tacggctaca ccaaacgcct gctcggacac atcctgcccg
     1921 actccgacac ttggggcggc aacggcatca tccacctcaa ttacagccgc accgaacaac
     1981 aacgcaatca cgaattgggt ttgcaaaaac accataacca cctctaccgc agcatcacgt
     2041 ctgcagaagc cgaaaaaatc gccggcatcc cgctgaacac gccctacgcc gaaccattat
     2101 gcggactcta ctggcaacac ggcgtatggc tcaatccgcc cgcattcgtc cgcaccctcc
     2161 tcagccatcc gctgatcgaa ctatatgaaa acacaacgtt aaccggcatt tcccacgacg
     2221 gagaaaagtg gattgcaagc acgccaaacg gcacatttac cgccacacac atcatctact
     2281 gcaccggcgc gcacagcccc tgcctgcccg aaaccaacct cgccgcccta cccctcaggc
     2341 aaatacgcgg acaaaccggc ctcacaccgt ccaccccgtt ttccgaacaa ctgcgttgcg
     2401 ccgtttcagg cgaaagctac atcagcccgt cgtggcacgg actgcactgc tacggcgcga
     2461 gttttattcc caacagcagc aataccggat ggaacgaagc cgaagaagcc tcaaaccgcc
     2521 aagcattggc acaccttaac cccgcccttg ccgaatcatt gtttgccgcc aacccaaacc
     2581 cccaaaaaca ccaagggcac gccgccatac gctgcgacag ccccgaccac ctccccgtcg
     2641 tcggcgcact cggcgacatt gccgccatgc ggcagactta cgccaaactt gcactggaca
     2701 aaaactaccg catcgacacc acatgcccat atctgcccaa tgcctacacc aataccgccc
     2761 acggcacacg cgggcttgcc accgccccca tctgcgccgc cgccgttgcc gccgaaatcc
     2821 taggcttgcc ccatctcttt tcacaacgcc tgcgccacgc cctacacccc aaccgcaccg
     2881 tcatccgcgc catcgtcaga aggcagaacc tgattcctta agcctttgcc gcatgccgtc
     2941 ccgaccacaa aaaatcatat tcaaataaac ataccaatcc tcacggtaaa atatcgaaaa
     3001 cacccataac gttttcttcc caaaataagg agtaaggaat gcctaacaaa ttgcctttgc
     3061 tgtccaagat gaattttccg taacattcga agacttcctt aaccctgcca gccgcttcgg
     3121 tttctgtacc ccctgttaga taaggaaggg atgaaaaagc cctgttactt gtatgaagca
     3181 aagggcgtta atttttgtta atcttccctt ctcaggaact caatatataa ggatgttaat
     3241 ttttatttcc cttctcgagg actcaatagt atgagaaatg agaaatgaga aatgagaaat
     3301 gagaaatgag aaatgagaaa tgagaaatga gaaatgagaa atgagaaatg cctccgatat
     3361 aatcggaggc attttgaatt ggcacgccca cggggaatcg aaccccggtt accgccgtga
     3421 aagggcgatg tcctaaccgc tagacgatgg gcgcatattg tattttctta ctggcgcacc
     3481 cggagcgatt cgaacgcccg accctctggt tcgtagccag atactctatc caactgagct
     3541 acgggtgcgg cacaatcact tgttcaagtg attaagaaag cgtgataata cggatttatc
     3601 tgagtcctgt ctattatttt agacttctat tttcagttat ttttgtaact gattgatttt
     3661 aaaataaact atttttcgac gcaagttgca gcaattctgc cgcatgttgc cgcgtcgtat
     3721 cggtaataac ttctccgccc aacatacggg caacctcttc gatccgttgt atttcatcca
     3781 atatactgat ttcgctgacg gtttgctctc cctcgctgtg cttgcgcacc cgccagtggt
     3841 tttctccgca ggatgcgact tgcggaagat gggtaacggc aagcacctga tgttttctgc
     3901 ccaacgcacg taatgccttg ccgaccattt cagccactcc ccctccaata ccggtatcga
     3961 cctcatcaaa aatcagggtg ggaacttggg tatattggct ggcaacaacc tgtaaggcaa
     4021 ggctgatacg cgccaattcg ccgccggagg cgactttgtt cagcgggcgg ggcggattgc
     4081 ctttgttagc ggcaacttga aattgaacct gctccaaacc gtgtgccgtc ggcgacgaag
     4141 gcaacaggac gatgtcgaaa cgcgcgcctt tcatggcaag gtgttgcata tgctcggtcg
     4201 tttcgctact caaacgccct gccgcctgat ggcgcatggc agaaaggatg tgggcagctt
     4261 cctgatattc ggcaaaattg tgggcaacat tatgctcgag cgcgtccaaa tcagcggcag
     4321 cttgaagact ttgcaggcgt tcttcgattt ctgccaactt ggcaggcaac tcttcgggtt
     4381 caatccggta tttccgcgcc atccccatca gttcacccat acgctgttcc tgtgccgcca
     4441 attcattggg gttgatgtca ctgtgtcctg ccacatcgcg catattggca ctgatttcgc
     4501 ccaattcggc ctcgatgctt gccaacatat tcaggctctc ggcaaagcgc ggctcgatgt
     4561 tttgcagatt ggccaatagt ttttgacatt gatagatatg gcgttggatg ccgttgtcgc
     4621 cgtcaatctt gcttccgact tcttcggcgg cctgcaacaa ttcggcagaa tgggcaaggc
     4681 tgtcgtggct ttggctgaga gcttcccatt cgccttgttt aatgtccaac tgattcagtt
     4741 cgttaaactg ccattccaga cgctcccgct cgataatgac ggcatcggcg tgttcctgcg
     4801 cctcttggag ggcttttttc gcattggccc agttttgata aagctgcctg acggtttccg
     4861 cctgcaccct gccgcccaca aatgcgtcca acaattcgcg ctgggcagct tcctgattaa
     4921 gcgaatgatg gtcgttttgc ccgtggatgt cgataagctg cccgccgacg gctttgagtt
     4981 gcgccaaggt agccgcctga ttgttgataa aactgcggct tttgcctttg gcatcgataa
     5041 tgcggcggat actgagttct tccccgccct catccaacag cccttgttca cgcaattctg
     5101 cttttaaagc aggcaaatgg gaaatatcga acaatgccga caactgcgct tcttttgcgc
     5161 cgctgcggac ttggctgtaa tcggccttat cgcccaacag cagaccaatc gcatccaaag
     5221 taatggactt gcccgcgccg gtctcgccgg tcaaaacggt aaaaccgctt tgaaaatcca
     5281 gattcagatt ttcgacaatg acaaaatcac gcaaagaaag tgttagaagc attgtgagga
     5341 ttccttagca attctaccga caaaagcaat atcaacttta gatatacgaa ttatatctaa
     5401 gattttttaa attaaaaata agtttttcct taattttgct tagtgtttgt tttt
//
LOCUS       DAXFWM010000095         5409 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000095 DAXFWM010000000
VERSION     DAXFWM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.095"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..225
                     /locus_tag="ACFI8B_002025"
     CDS             <1..225
                     /locus_tag="ACFI8B_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3835148.1"
                     /translation="GFTRCRINRSKEFADRRNHINGIGNFWNQAKRALRKYNGIDRKP
                     FPPLLRECEFRFNFGTPPRQLKILRDRCGI"
     gene            351..1172
                     /locus_tag="ACFI8B_002026"
     CDS             351..1172
                     /locus_tag="ACFI8B_002026"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC3835149.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1258..1920)
                     /locus_tag="ACFI8B_002027"
     CDS             complement(1258..1920)
                     /locus_tag="ACFI8B_002027"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC3835150.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1958..2785)
                     /locus_tag="ACFI8B_002028"
     CDS             complement(1958..2785)
                     /locus_tag="ACFI8B_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC3835151.1"
                     /translation="MKPDIYALPERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            3167..3790
                     /gene="lolA"
                     /locus_tag="ACFI8B_002029"
     CDS             3167..3790
                     /gene="lolA"
                     /locus_tag="ACFI8B_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC3835152.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            4063..5202
                     /locus_tag="ACFI8B_002030"
     CDS             4063..5202
                     /locus_tag="ACFI8B_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3835153.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENAEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
BASE COUNT         1252 a         1472 c         1498 g         1187 t
ORIGIN      
        1 ggttttaccc gttgccgcat caaccgttcc aaggaatttg cagaccgtcg gaaccacatt
       61 aacggcattg ggaacttttg gaatcaggca aaacgcgcct tgcgaaaata caacggaatc
      121 gatcgtaaac ctttcccgcc gttgttgagg gaatgcgaat ttcgatttaa cttcggcaca
      181 ccgccccggc agctaaaaat cctgcgggat cggtgtggaa tttagggata atctagtaca
      241 gccccttgtt ttttggggtg taatccgagg taggggcggc tggggtgctt ctcccttgtc
      301 tgccgctgct gttatgatga tatttttatt ctgtatttaa ggagggggtg atgagcagtc
      361 cgcgccaagt gttttatatt tccgaccgca ccggtctgac tgccgagaat atcggcgagg
      421 ctttgctgaa ccagtttggc aatctgtcgt tcaaacgcca tacgcatccg tttgtcgata
      481 cgccggaaaa ggcgcgcgcg gtggtggaga aggtcaatcg gagccggcag gaaaacggtc
      541 agcgtccgat tgcgtttgtc agtgtggttg atgacgaaat ccgccggatt attaaagggg
      601 cggatgcttt tcagattaat ttctttgaga cttttttggg actgttggag aaggaactca
      661 ataccgaagc aacggtatcc gggcaggggc atcacagtat cggcaatacg aagcgttacg
      721 atgcgcgtat ggaggcggtc aatttttctt tgaaccatga tgacggggtc agcgataaga
      781 accttcagga ggcggatgtg attctgatgg gcgtgtcccg ttcgggcaaa acgccgacct
      841 gcctgtatct ggcgttgcaa tacggtatcc gtgcggcaaa ctatccgctg attcccgacg
      901 atttggaatc ggccgatctg ccgcgtatgg tcaagccgta taaagacaaa ctgttcgggt
      961 tgactatcca gccggaacgt ttgcaggcca tccgtcagga acgccgcccg aattcggctt
     1021 atgcgcgcat cgacacctgc cgcagcgaag tggcggacgc gcagagtatg ttcagacggc
     1081 atggcattcc gtttgcgaat acgacggaca agtcggttga ggaactggcg gtacacatcc
     1141 ttcaggcgtg caagctcaaa cgcaggtttt gacgggcttt gattcggttt gaaggtggaa
     1201 atgccgtctg aaatcaggtt tcagacggca gttttatgtt tgtggggcgg atatttttca
     1261 gggctgtatt ttgtccagac attcgagcag atcgagcggc gtgcggatgt ggaaatccgc
     1321 ctgccatgag ccggtatcgt cttcatcgga aatatagccc cattcggcga ggacggtttt
     1381 cataccggcg ttgcgtccgg cttggatgtc gcgttccgcg tcgccgacgt agagggtgtg
     1441 ttgcgggtcg gcgtggattt ttccgcaggc gtgcagcatg ggtttgatgc tgggtttggg
     1501 ttcgccgcag gtgtcgccgc tgacgacggt ggcgggcgga acggcgaatc cgagtttggg
     1561 aacgagtttg tcggtgaagc gcatgggttt gttggtgatg atgccccatt tgatgccgcg
     1621 tctgtcgagt tcggcgatga gttcgttcac gccgtcgaag agagtggtgt cttgggcgta
     1681 gcggctgtcg tattcctcca gatattcggt gcgccatgcg gtatagtcgg ggtgttcggg
     1741 cgtgatgttc gcgccgagtt tgagcaatcc tgccgcgccg tggctggctt gggtgcggat
     1801 ttcgtccatg cttttttcgg gcagtccgtg gcgggcgagt tgggtgttga gtgcgccgcc
     1861 gaggtctagg gcggtgtcgg cgagcgtgcc gtcgaggtcg aacaatacgg cttgtatcat
     1921 gtgttttcct tttttataaa gtgcgggacg aaaggtttca gacggcatgt ttattttgtt
     1981 tcaaaccctg ctcgaaatct tccaacatat ccaattcgaa gcggctgaag cccgcttttt
     2041 cccgcgcttc gatgttcaca tagccgcgga agataaacat atcgtaacgg gcaatcagac
     2101 tgcggaacag ggcgacaggt tccaaaccgc gttcgcggca gaggtgttga taccaccggt
     2161 tgccgatggc gacgtgtccc acttcgtcgc ggtaaatgat gtccaacacg ccgcaggttg
     2221 ccgaatcgcc gcgctgctcc accttcgcgc gtatccccgg cgtaacgtcc agtccgcgcg
     2281 cctccaacac gcgcggtacc aacgccatac gcaacagcgg gtcgtaggcg gttttgtacg
     2341 ccatatccca caaatggttg tgcgcctcga aactgccgta atcgaagccg aaagcgcgca
     2401 atctgtcgcg caccaggcgg aagtggtaca cctcctcctt cgccactttc acccagtcgc
     2461 ggacaaactg aaacggcagc gtgcggaaac ggtatgccgc gtccaaagcc agattgacgg
     2521 cgttgaactc gatatgcgta atcgcgtgca gcatcgccgc atagccttct gccgtgttca
     2581 ttttgcgcgg ggtcagttgc gagggcgcga ccaaaacagg cgtgtccggc cgtcccgcgc
     2641 gggggaagtc cgccggcggc gcgtttgcct ccgccccgtc cgcattttga acggcggcaa
     2701 acgcctcatc cgtcagccgt cctttttcat cggggtcgcc cgaaagcagg gcgcgttccg
     2761 gcaaagcata aatatcgggt ttcatctcaa gtccgccgtg ttcggaaaac gaatattata
     2821 gcgtttaaaa aaaacaagat gaggcatata atctccgcga ttcggcattc cgcgcccaaa
     2881 ccgtcaaata tagtggatta acaaaaacca gtacggcgtt gccccgcctt gccgtactat
     2941 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataacgcggc
     3001 atacaattga aagggcagcg tggcgcgcct gctttttccg agcggtcaaa aaaatcagcc
     3061 ctcggaaaac gcggtttgca aaatgcaaac cgcccgtaac gccgcccgta tgattgtttt
     3121 gccgcgccga tactttgcgc cacacccatc ccgacaagga aaaataatga tgaaaccgca
     3181 caacctgttc caattcctcg ccgtttgctc cctgaccgtc gccgtcgctt ccgcacaggc
     3241 gggcgcggtg gacgcgctca agcaattcaa caacgatgcc gacggtatca gcggcagctt
     3301 cacccaaacc gtccaaagca aaaagaaaac ccaaaccgcg cacggcacgt tcaaaatcct
     3361 gcgcccgggc ctcttcaaat gggaatacac tttgccctac aaacagacta ttgtcggcga
     3421 cggtcaaacc gtttggctct acgatgttga tttggcacaa gtgaccaagt cgtcccaaga
     3481 ccaggccatc ggcggcagcc ccgccgccat cctgtcgaac aaaaccgccc tcgaaagcag
     3541 ttacacgctg aaagaggacg gttcgtccaa cggcatcgat tatgtgcggg caacgcccaa
     3601 acgcaacaac gccggctacc aatacatccg catcggcttc aaaggcggca acctcgccgc
     3661 catgcagctt aaagacagct tcggcaacca aacctccatc agtttcggcg gtttgaatac
     3721 caatccccaa ctctcgcgcg gcgcgttcaa gtttaccccg cccaaaggcg tggacgtgtt
     3781 gagcaactga tgccggccgc cccgatgccg tctgaacgcc gccgaggctt cagacggcat
     3841 tttcacgcag gcggaacaat gtcccgcatt atcggccgat cgggcaacgg aacggcaaat
     3901 ccgtgaaaat taacggttgc gccccggctg ttttttccgt ttaatgcaaa ccttgctgca
     3961 ccaagggcca agaaagccga ccggccgccc ccacagcttc cgatgcaggc ggcccgtccg
     4021 tccctgcaat gttttttatt tttgaacgaa aggtcgaaaa ccatgaaaaa aacactggtg
     4081 gcggcggcaa tcctgagcct tgccttgact gcgtgcggcg gcggaagcga taccgccgcc
     4141 caaaccccct ccgccaagcc cgaagccgaa caatcgggca aactcaacat ctacaactgg
     4201 tcggattatg tcgatcccga aaccgtcgcc gcctttgaaa aagaaaccgg cattaagatg
     4261 cgttccgact attacgacag caacgaaaca ctggaggcaa aagtcctgac cggcaaatcc
     4321 ggctacgacc tgaccgcgcc gtccatcgcc aacgtcggcc ggcaaatcaa agcgggcgcg
     4381 tatcagaaaa tcgacaaggc gcaaatcccc cattacggca acatcgataa agatttgctg
     4441 aaaatgatgg aagccgtcga tccgggcaac gaatacgccg tcccctattt ctggggcatc
     4501 aacaccttgg caatcaacac gcggcaggtg caaaaggcat tgggtacgga caagctgccc
     4561 gaaaacgagt gggatttggt gttcaaaccc gaatacaccg ccaaactcaa atcctgcggc
     4621 atcagctatt tcgacagcgc aatcgaacag attcccttgg cgttgcacta tttgggcaaa
     4681 gaccccaaca gtgaaaatgc cgaagacatc aaagccgccg tcgatatgat gaaagccgtc
     4741 cggggcgacg tgaaacgctt cagctcttcc ggctatatcg acgatatggc ggcgggcaac
     4801 ctgtgtgccg ccatcggtta cggcggcgat ttgaacattg ccaaaacccg tgccgaagaa
     4861 gccgcaaacg gcgtggaaat caaagtattg accccgaaaa ccggcgtagg cgtgtgggtg
     4921 gattccttta tgattccgcg cgacgcgcaa aacgttgcca acgcccaccg ctatatcgac
     4981 tacacgctcc ggcccgaggt ggctgcgaaa aacggcagct tcgttaccta cgcgcccgcc
     5041 agccgtcccg cgcgcgagct gatggatgaa aaatacacct ccgacgcatc gattttcccg
     5101 accaaagaac tgatggaaaa aagtttcatc gtatcaccca aatccgcaga atccgtcaaa
     5161 ctgggcgtga agctgtggca agggctcaaa gcgggcaaat aaccggaatc cctgccgtct
     5221 gaaacctttc gggcggcagg aaacggcgtg tccgcattaa gtcaggatta ggattattta
     5281 gaaagatgag atggatatga atttaagtat tgtcgttcct atttataatg tcgaaagtta
     5341 tttggaagcg tgtttaaatt ctatagaacc tatattaagt aatgaaaatg tcgaacttat
     5401 tcttgtgaa
//
LOCUS       DAXFWM010000096         5256 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000096 DAXFWM010000000
VERSION     DAXFWM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.096"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            186..2303
                     /locus_tag="ACFI8B_002031"
     CDS             186..2303
                     /locus_tag="ACFI8B_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3835154.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     HDVPSKKPEARKDQGGYGFAMRFKRRNRHPMAMPKENEVKLKDDDWEATGLPGDPKDL
                     PGRQKSVIDEVSANGNNDIYFSPYLKPSNHQNSSINGSANQPKNEVKDYKNFEYVYSG
                     WFYKHAEPIIDGIQNKLQQGDDGYIFYHGKDPSRQLPASEKVIYKGVWHFVTDTKQGQ
                     KFNDILETSKKQGDSYSGFSGDEGETTSNRTDSNLNDKHEGYGFTSNFEVDFNNKKLT
                     GKLIRNNKVTDAAASNGYTTEYYTLDATLRGNRFSGKATATDKSSNEQAKLHPFVSDS
                     SSLSGGFFGPQGEELGFRFLSDDNKVAVVGSAKTKDKNANGNTAAAGTAGAAGMSSED
                     TKLTTVLDAVELKSDGKKVEDLDNFSDATRLVVDGIMIPLLPTESGNGQADKGKNGKT
                     AFIYETTYTPESDEKDTQTGMAANGVQTVSNAAGGTSGKTKTHYEVQACCSNLNYLKY
                     GLLTRKTAGNTGGGGKGSQAAQTAQGAQSMFLQGERTDEKQIPKDENVVYLGSWYGYI
                     ANGTSWSGNASNTTSGNKAEFTVNFDKKNITGMLTAANRQEATFTIDAMIESNGFKGM
                     AKTGNGGFAPDQNSSTGTHKVHITSAAVQGGFYGPKAEELGGWFAYPGNGQTKNAQAS
                     SGNGNSAGSATVVFGAKRQELVK"
     gene            2390..5128
                     /gene="tbpA"
                     /locus_tag="ACFI8B_002032"
     CDS             2390..5128
                     /gene="tbpA"
                     /locus_tag="ACFI8B_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFC3835155.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDDSSDYAHFIVEGECKNGGYETCKTRP
                     KKDVAGEDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKYGLIRGYGKYAGDHRYGGLITNSENGAQVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKYCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSN
                     LRHQDYYYQSANRAYSLKTPPKNGKNSPNGDKDNPYWVTIGRGNVVTRQICLFGNNTY
                     TDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWN
                     TGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEAGIVFK
                     GDFGNLEASWFDNAYRDLIVRGYEVQIKDGKEGVKGDPAYLNAQSARITGINILGKID
                     WNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSGYDQPE
                     GKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTVKKH
                     FTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
BASE COUNT         1483 a         1360 c         1360 g         1053 t
ORIGIN      
        1 gccctccccc gcgcagggga gggagcggat tgcagcaggt ttggcggttg caggcggttt
       61 taaaagcagc gcgggaatga cggtattttg gtttatttac taaaaatata aaaaagcatt
      121 tgcaaatttt ttaaaaataa ataaaataat aatccttatc attctttaat tgaatcgggt
      181 ttgttatgaa caatccattg gtgaatcagg ctgctatggt gctgcctgtg tttttgttga
      241 gcgcttgtct gggcgggggc ggcagcttcg atcttgattc tgtcgatacc gaagccccgc
      301 gtcccgcgcc aaagtatcac gatgttcctt ccaaaaaacc ggaagcccga aaagaccaag
      361 gcggatacgg ttttgcaatg cgcttcaagc ggcggaatcg gcatcctatg gcaatgccta
      421 aagaaaatga ggttaaatta aaggatgatg attgggaggc gacaggattg ccaggcgatc
      481 ccaaagactt acctgggcga cagaaatcgg ttattgacga agtgtcagcc aacggcaaca
      541 acgatattta tttctctccc tatctcaaac catcaaacca tcaaaacagc agcattaatg
      601 gcagtgcaaa ccaaccaaaa aatgaagtaa aagattataa aaatttcgaa tatgtttatt
      661 ccggatggtt ttacaaacac gctgaaccaa tcatagatgg gattcaaaac aaattgcaac
      721 aaggcgacga cggttatatc ttttatcacg gtaaagaccc ttcccgacaa cttcccgctt
      781 ctgaaaaagt tatctacaaa ggcgtatggc attttgtaac cgatacgaaa cagggacaaa
      841 aatttaacga tattcttgaa acctcaaaaa agcaaggcga cagctacagc ggattttcgg
      901 gcgatgaagg cgaaacaact tccaatagaa ctgattccaa ccttaatgat aagcacgagg
      961 gttatggttt tacctcaaat tttgaagtgg atttcaataa taaaaaattg acgggcaaac
     1021 tgattcgcaa caataaagtt acagacgctg ctgctagcaa cggatatacc accgaatact
     1081 acacccttga tgcgacgctt aggggcaacc gcttcagcgg caaggcgacg gcgaccgata
     1141 aatccagcaa tgaacaagcc aaactacatc cctttgtttc cgactcgtct tctttgagcg
     1201 gcggcttttt cggcccgcag ggtgaggaat tgggtttccg ctttttgagc gacgacaaca
     1261 aagttgccgt tgtcggcagc gcgaaaacca aagacaaaaa cgcaaatggc aatactgcgg
     1321 cggctggcac cgcaggtgcg gcaggcatgt cgtctgaaga cactaagctg accacggttt
     1381 tggatgcggt cgagctgaaa tcggacggta agaaagtcga agatctcgac aacttcagcg
     1441 acgctacccg actggttgtc gacggcatta tgattccgct cctgcccacc gaaagcggga
     1501 acggtcaggc agataaaggt aaaaacggca aaacagcctt tatctacgaa acaacctaca
     1561 cgccggaaag cgatgaaaaa gacacccaaa caggtatggc ggcgaatggc gtgcaaaccg
     1621 tttcaaatgc ggcgggcggc acaagtggca aaacaaaaac ccattatgaa gtccaagcct
     1681 gctgttccaa cctcaattat ctgaaatacg ggttgctgac gcgcaaaacc gccggcaaca
     1741 cggggggagg cggcaaaggc agccaagccg cccaaacggc acagggcgca caaagtatgt
     1801 tccttcaagg cgagcgcacc gatgaaaaac aaattccaaa agacgaaaac gtcgtttatc
     1861 tggggtcttg gtacgggtat attgccaacg gcacaagctg gagcggcaat gcttccaata
     1921 caacgagtgg caacaaggcg gaatttaccg tgaatttcga taagaaaaac attaccggca
     1981 tgttaaccgc tgcaaacagg caggaggcaa cctttaccat tgatgccatg attgagagca
     2041 acggctttaa aggtatggcg aaaaccggta atggcggctt tgcgccggat caaaacagca
     2101 gcaccggtac acataaagtg cacatcacaa gtgccgcggt gcagggcggt ttttacgggc
     2161 ctaaagccga agagttgggc ggatggtttg cctatccggg caatggacaa acgaaaaatg
     2221 cgcaagcttc atccggcaat ggaaattcag caggcagcgc gaccgtggta ttcggtgcga
     2281 aacgccaaga gcttgtgaaa taagcacggc tgccgaacaa tcgagaataa ggcttcagac
     2341 ggcatcgttc ctgccgattc cgtctgaaag cgaagattag ggaaacacta tgcaacagca
     2401 acatttgttc cgattcaata ttttatgcct gtctttaatg actgcgctgc ccgcttatgc
     2461 agaaaatgtg caagccggac aagcacagga aaaacagttg gataccatac aggtaaaagc
     2521 caaaaaacag aaaacccgcc gcgataacga agtaaccggt ttgggcaaat tggtcaaaac
     2581 cgccgacacc ctcagcaaag aacaggtact cgacatccgc gacctgacgc gttacgaccc
     2641 cggcatcgcc gtcgtcgaac aggggcgcgg cgcaagctcg ggctactcga tacgcggtat
     2701 ggacaaaaac cgcgtctcct tgacggtgga cggcttggcg caaatacagt cctacaccgc
     2761 gcaggcggca ttgggcggga cgaggacggc gggcagcagc ggcgcaatca atgaaatcga
     2821 gtatgagaac gtcaaggctg tcgaaatcag caaaggctca aactcggtcg aacaaggcag
     2881 cggcgcattg gcgggctcgg tcgcatttca aaccaaaacc gccgacgatg ttatcgggga
     2941 aggcaggcag tggggcattc agagtaaaac cgcctattcc ggcaaaaacc gggggcttac
     3001 ccaatccatc gcgctggcgg ggcgcatcgg cggtgcggag gctttgctga tccgcaccgg
     3061 ccggcacgcg ggggaaatcc gcgcccacga agccgccgga cgcggcgttc agagcttcaa
     3121 caggctggcg ccggttgatg acagcagcga ctacgcccat ttcatcgttg aaggagaatg
     3181 caaaaacggg ggttatgaaa cgtgtaaaac gagaccgaaa aaagatgttg ccggcgaaga
     3241 cgaacgtcaa acggtttcca cccgagacta cacgggcccc aaccgcttcc ttgccgatcc
     3301 gctttcatac gaaagccggt cgtggctgtt ccgcccgggt tttcgttttg agaataagcg
     3361 gcactacatc ggcggcatac tcgaacgcac gcagcaaact ttcgacacgc gcgatatgac
     3421 ggttccggca ttcctgacca aggcggtttt tgatgaaaat aaaaaatacg gattgataag
     3481 aggctacggc aaatacgcgg gcgaccaccg atacggcggg ctgattacca acagtgaaaa
     3541 cggtgcgcag gtgggcgcgg aatacggtac gggcgtgttt tacgacgaga cgcacaccaa
     3601 aagccgctac ggtttggaat atgtctatac caatgccgat aaagacactt gggcggatta
     3661 tgcccgcctc tcttacgacc gtcagggcat cggtttggac aatcattttc agcagacgca
     3721 ctgttccgcc gacggttcgg acaaatattg ccgcccgagt gccgacaagc cgtcttccta
     3781 ctacaaatcc gaccgcgtga tttacgggga aagccacagg ctcttgcagg cggcattcaa
     3841 aaaatccttc gataccgcca aaatccgcca caacctgagc gtgaatctcg gttacgaccg
     3901 cttcggctct aatctccgcc atcaggatta ttattatcaa agtgccaacc gcgcctattc
     3961 gttgaaaacg ccccctaaaa acggcaaaaa cagccccaac ggcgacaagg acaaccccta
     4021 ttgggtcacc ataggcaggg gaaatgtggt tacgaggcaa atctgcctct ttggcaacaa
     4081 tacttatacg gactgcacgc cgcgcagcat caacggcaaa agctattacg cggcggtccg
     4141 ggacaacgtc cgtttgggca ggtgggcgga tgtcggcgcg ggcttgcgct acgactaccg
     4201 cagcacgcat tcggacgacg gcagcgtttc caccggcacg caccgcaccc tgtcctggaa
     4261 caccggcatc gtcctcaaac ctgccgactg gctggatttg acttaccgca cttcaaccgg
     4321 cttccgcctg ccctcgtttg cggaaatgta cggctggcgg tcgggcgata aaataaaagc
     4381 cgtcaaaatc gatccggaaa aatcgttcaa caaagaagcc ggcatcgtgt ttaaaggcga
     4441 tttcggcaac ttggaggcaa gttggttcga caatgcctac cgcgatttga ttgtccgggg
     4501 ttatgaagtg caaattaaag acggcaaaga aggagtcaaa ggcgacccgg cttacctcaa
     4561 tgcccaaagc gcgcggatta ccggcatcaa tattttgggc aaaatcgatt ggaacggcgt
     4621 atgggataaa ttgcccgaag gttggtattc tacatttgcc tataatcgtg tccgtgtccg
     4681 cgatattaaa aaacgcgcag accgcaccga tattcaatca catctgtttg atgccatcca
     4741 accctcgcgc tatgtcgtcg gctcgggcta tgaccaaccg gaaggcaaat ggggcgtgaa
     4801 cggtatgctg acttattcca aagccaagga aatcacagag ttgttgggca gccgggcttt
     4861 gctcaacggc aacagccgca atacaaaagc caccgcgcgc cgtacccgcc cttggtatat
     4921 tgtggatgtg tccggttatt acacggttaa aaaacacttc accctccgtg cgggcgtgta
     4981 caacctcctc aaccaccgct atgttacttg ggaaaatgtg cggcaaactg ccggcggcgc
     5041 agtcaaccaa cacaaaaatg tcggcgttta caaccgatat gccgcccccg gccgcaacta
     5101 cacatttagc ttggaaatga agttctaaac gtccgaacgc cgcaaatgcc gtctgaaagg
     5161 cttcagacgg cgttttttac acaatcccca ccgtttccca tcatccccga tacaccgtaa
     5221 tcccgaaacc cgtcattccc gcgcaggcgt gcatcc
//
LOCUS       DAXFWM010000097         5243 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000097 DAXFWM010000000
VERSION     DAXFWM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.097"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..3215)
                     /locus_tag="ACFI8B_002033"
     CDS             complement(<1..3215)
                     /locus_tag="ACFI8B_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC3835156.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLTYLNIWDNFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAG
                     YEQIHRALLTGLIANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSA
                     SPLPNPLPQEREQSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPP
                     FSDGLRPANPQQTTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDD
                     PKPKKQPASQKGRLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEE
                     QFPKHWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQ
                     LQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     NEWAAFRLPEHCYFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDN
                     VTAWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEK
                     AFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPG
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQ
                     GQPVSDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKM"
     gene            complement(3281..4858)
                     /gene="lpt3"
                     /locus_tag="ACFI8B_002034"
     CDS             complement(3281..4858)
                     /gene="lpt3"
                     /locus_tag="ACFI8B_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC3835157.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            complement(5085..>5243)
                     /locus_tag="ACFI8B_002035"
     CDS             complement(5085..>5243)
                     /locus_tag="ACFI8B_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFC3835158.1"
                     /translation="TYKNDAIAWGIWNNQLSEDEVENYLKNFVNKKDTHYKRLLCMFD
                     YKKYADTV"
BASE COUNT         1046 a         1221 c         1553 g         1423 t
ORIGIN      
        1 cacatcttca acagccgctt taccgacacc ggatacggcg tcttcaactc ctgcgcgaac
       61 agcgacactc tcaattcttc aatcatccat ttaaacgcgg cgaggccgtc tgaaacgggc
      121 tgtccttgtt tcgccaagcc gtcagttttt tcctgccaca tttgttccag ctcttggata
      181 tcggcttcgc gggctgcgtc gcgggcgggg ttgctgctgt atttttcgag gcgcagggtc
      241 atggctttga ggtagatggg gaggcgcggc cattgtgccc acggggtgcg ggtggcgaag
      301 ccgggtgcaa gcagggtttg caggcgtagt cttaggaggt gggtcaatgg gtgtttgccg
      361 agtttgccgt tcagttcggc gtaggcggct gcggtttcct gcaggtagcg gctgagggct
      421 tctttgacgg cgggcaggcg gctgcgggcg cgtttgattt gttctttgaa ggctttttcg
      481 ttgcgcggca gcttgtcttc gccgataaag gcgcggtcgc agacggcttg ggtgaggtcg
      541 tcgcgcaggg tgtcggcgtt gatgtgtttg agcagcatgg cggcttgggt gaagccttgg
      601 atgcctttgt tcagatcttt aacttgttct tttagttgta gtttcatgag ttcaatcacg
      661 cctaatctgt gggcgtgtcc ggcggcggca gacgtgtcga acaggcgcag ggcgatgcgg
      721 ccgtcttttt ctttttgcag gccgaggtag ccggtgagct gttgtttgcc gcgggcgaat
      781 ttgatggatt cgggcaggat gccgatgtcc catgcggtaa cgttgtcacg ctcgaattct
      841 tgggtgttgt cgcggaaggt ggtggtggcg gctttgccaa gttgttgttg gatttggatt
      901 aaatcgcgac ccatggctaa ctcttgtccg ccgtcgtcga tgatgcggag gttgaaatag
      961 cagtgttcgg gcagcctgaa cgcggcccat tcgttttggt tgatttgctc gagtatgcgg
     1021 atgtcgcctg cggttttggc gatggcttgg gcgagctggg gcaggatggg ggcgttgcgg
     1081 tcggggtttt ggcttaaaaa ttgggtgatg aattcgggta cgggtacgca gatgcggcgg
     1141 atttgtttgg gcagtgcttt gatttgtagc tggatttttt cgcgtatcat gccgggcacg
     1201 agccattcga gggcggcggg gctgatgcgg ttgagcacgg tgagcggcag ggtgagggta
     1261 acgccgtcga gcgggtggtg cggctcgaag cggtagctga gtttgaattt gccgtctgcg
     1321 gtttgccagt gtttggggaa ttgttcttcg gtgatgtgcg cggcggcgtg ctgcatgagg
     1381 tcgtcgcggc tgaggaacaa caggcgcggg ttgtcgcgct cggcggtttt gagccaggct
     1441 tcaaaggtgc ggatatcggc aaggggcaaa ggtttcagac ggcctttttg agatgcaggc
     1501 tgctttttgg gtttggggtc atctgaaact gttaaagccg aggcactctg ttccctctcc
     1561 tgtgggagag ggtttgctgc ggttgcggaa aagttggttt gtgcggcaac tgttttgccc
     1621 tctccccagc cctcccccac gggggagggg gtggtttgct gggggtttgc aggacgcagg
     1681 ccgtctgaaa atggcggggt gtagttttca gatagccttt gggagtgcag gctgctttcg
     1741 gttttagttt tagcttcgca gaaattcagc cttgcttcgc aagacattgt tttcagactg
     1801 cctgaaaccg ttgaaactga ggcactctgt tccctctcct gtgggagagg gttagggaga
     1861 gggcttgctg aggttgcgga aaagttggtt tgtgcggcaa ctgttttgcc ctctccccag
     1921 ccttccccca cgggggaggg ggtggtttgc tgcggatttg caggacacag gccctctgaa
     1981 accgaatccg ccatataaaa atcgggcagc cgttcgtgat aaaacgcaaa cagggcttcg
     2041 tcatcgacca atacgtcttg cttgcgcgat ttgtgttcga gttcggtaat ttctttaatc
     2101 agctttttgt tgtggacaaa aaaatccgct ttcaaatcgt attcctgcgc caccaacgcg
     2161 ctgcggataa agatttcacg tgcttcttcg ggcgcaactt taccgtaaga cacggggcgg
     2221 cgcggcaata cggtcaggcc gtaaagcgtt acgcgttcgc tggcgacgac ttcgccgcgt
     2281 ttttgttccc agtgcggctc gaaataatgg tagcggacaa ggtgcggggc ttcctgctcg
     2341 atccattcgg gctggataac ggcgacgtcg cgcgcgtaaa ggcgcgtggt ttcaaccaat
     2401 tctgccgcca tcacccattt gggcttggct ttgaacaggg cggaggcggg gaaaaggtgg
     2461 aagcggctgc cgcgcgcgcc ggtgtagtcg ttgctgtcgg gcgatttcat gccgacgttg
     2521 gcgatgaggc cggtgagcag ggcgcggtgg atttgttcgt agcccgcttc tttggtagcg
     2581 cggatttggg tgcggtgttg tttcttatcc agttgttttt gtttgagttt ggcagatagg
     2641 tcttggtcgc ccgcattctc agacgacgtc agttgcttga cttcgggagg tcgtctaaac
     2701 gcagcttctt tggtggtcaa gcccatttca atcgcggttt gggcaagctg gtggtgcagc
     2761 tcgcgccact cgcgcatcct caggtgcgac agaaaatatt gacggcacca ttgcaccaac
     2821 tgcttgttgg acaaaccttt gtcgcgctcg cgctggaaat tgtcccaaat attcaggtag
     2881 gtaaggaaat cggattgctt gtcggtaaag cgttcatgcg ccttggccgc ggcatcgcgc
     2941 gcttccagcg gccgttcgcg cgggtcttga atcgacagcg cagacgcaat caccaatatt
     3001 tccgccatgc agtcgtgttt cttcgccgcc agcaaaatgc gcgcaatttt cggatcgatg
     3061 ggcaggcgcg ccatttgttc gccgagcttg gtcaggcggt agcgcgtgtt ggtaccgata
     3121 tggcccgaac tgttttgcag gccgtctgaa acattggccg ggttggatcg ggcatccatg
     3181 cctgatccgc gtacttggtt tttcatattt tgcatagtct tgtcggtcgg gcatcttccc
     3241 cgacatcatc taaatttgtc tttattggtt ttcacgctgc ttattgcgga taaacatatt
     3301 ccgccttgcc gttgcgaatg ttcaagctgc ctgcatcgcc cgtaatcagg ttgcctgtta
     3361 ccgagccttc gcgacaacct gaaaccggca tatcgtagcc caacgtgtga atcaggaacg
     3421 ttgaaagctg ctgatggaag gcaatctcgc aaggcgcaaa agcctggttg gcagcctgtt
     3481 gcacggcctt atccgggctg tacaaaacca gaggcacaat atagctgtcg ggctgcaccg
     3541 tgccttgatt gtagatatct tggcgcacat actggccatg atcggaggta taggcaaaca
     3601 gccagttgcc gtcaggctgc ttttgcagct gctcgaatac ggtttgaatc atttggtcgg
     3661 ttttgtggat ggtgttgtcg tacttatcca caatatcggc ttcgccgaat actttatctt
     3721 gaggctgcaa caatgcgccg tatggggcgt gcgaaccgcg ttggtgcaac acgataaaat
     3781 gcctgccctg ctgcaaattg attttgtcga acaacggcag cagcttctca tcgggcatat
     3841 tgtcgccgtt gccgtagcca agttgcgtcg gctgaatcag atggtctatc catttcttac
     3901 cgattaagtt caaaattgcc atttggtttt cagcctgggc actgtaaaaa tacgtttcat
     3961 agccctgctc tttggcgagg cggaacatat tggtatcgcc gccgctgatt tgttccaagc
     4021 cgttggcgtg cggtatgacg ttaaagaaac tgggcaggga tactgccgtc ataaagcctg
     4081 cggaataact ttgtttcaca atcggcttaa aatcggcttg cgacagccgg gttaaaaacg
     4141 gcgaagtttc gcgcccgtaa ccaaacaatt tcaaatgcgc cgcgctttcg ctttcgccca
     4201 taatcaggac gatattttga atactgcctt gcccgatttt gcttggagca ggctgtttga
     4261 acacagggat cttgcttaaa tcaaacaact gatacggcaa cacgcgtccg acaaaataac
     4321 cgaagctgaa ataattggct ttgatgcggc tgtatgtcgg tttgggcgaa ataccgtgct
     4381 cttgtttcgt gtcgaacgaa cgcacgaaaa tcatcagcat taggaaggca aacagtatat
     4441 cggcagaaaa atgcgtctta cggcggaact tggcaaggct gcaaaacagc acgacttccg
     4501 ccacgcccca caacgcaggc agccacaact tatccaacat cgacgcgccc gcgctgccga
     4561 cttcggtaac ctctttcagc atcagccaat agttaatacc cgtcatccag ctttgataaa
     4621 ccgcgtaatg cacattgttg gcaataatgc tgaacgcgaa aaacaccgca atcagcagcc
     4681 gcgaagcctt ataacgcgca aacagataca gcgcagcaat cataaatgtc agcgcaaacg
     4741 tttccgccat ttttgcagcc ggtagggttt caattccgaa tacaaagcga taggcgattt
     4801 cgctggcggt aagtagggat gaatacagaa agagaacgaa aagggatttt ttcataatgg
     4861 gaagttcaca ataaaagttg caggacgggt tttggggctc ggcaacggta ttcttcagca
     4921 gctatctgtt tacagattgt ttttcaaaaa acgaggttgt ctgaaagtgg tttgtcattt
     4981 gtcggattgt gttgggcatg aatgcctgag ctgcgtgctt gatttttagt gttttgcata
     5041 gttttgtagg tcggacataa atgcccgacc ttatttttta gagtttagac agtatccgca
     5101 tattttttat aatcgaacat acatagtagt cttttatagt gcgtatcttt tttattcaca
     5161 aaatttttta aataattttc aacctcatct tctgaaagtt ggttattcca tattccccaa
     5221 gcaatcgcat cgtttttata tgt
//
LOCUS       DAXFWM010000098         5209 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000098 DAXFWM010000000
VERSION     DAXFWM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.098"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..879
                     /locus_tag="ACFI8B_002036"
     CDS             <1..879
                     /locus_tag="ACFI8B_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3835159.1"
                     /translation="PVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQPSL
                     SKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEVTSR
                     PDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLSARE
                     FLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDDRRK
                     LLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKNLRG
                     SCFNDMASLLQKEVQS"
     gene            876..2396
                     /locus_tag="ACFI8B_002037"
     CDS             876..2396
                     /locus_tag="ACFI8B_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFC3835160.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAVKQDVNLHQKEVSENNTESKDSDMEFVPAKVNGTKADAEDEP
                     KEAEPITLEQTSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAG
                     NAIYAIDVLHEEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDK
                     YDEYTNCGIGNKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            2386..2991
                     /locus_tag="ACFI8B_002038"
     CDS             2386..2991
                     /locus_tag="ACFI8B_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835161.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            3000..3743
                     /locus_tag="ACFI8B_002039"
     CDS             3000..3743
                     /locus_tag="ACFI8B_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835162.1"
                     /translation="MDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVRQFAQ
                     AVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGILLNR
                     RTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAFVSIL
                     YLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGLIESC
                     VDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            3743..>5209
                     /locus_tag="ACFI8B_002040"
     CDS             3743..>5209
                     /locus_tag="ACFI8B_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835163.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGRS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAM"
BASE COUNT         1522 a         1039 c         1126 g         1522 t
ORIGIN      
        1 cccgtaatat tgtctgcagc cgctacaaaa ggtggcgtag gtaaaacaac actcattgct
       61 aatgtctctg ctgttctcgc tgatatcgga ctgcgtgtct taatgattga ctgcgatgtt
      121 cagccatcat tgtcaaaata ctatccaata agccaccgcg ctccaaatgg tattgttgaa
      181 ttacttctcg gtgaaaatac cgaagaaatc atccgctcga caatttccaa taccgttttt
      241 ccgaatttag acattgttat ttccaataac atttctgctt ttgtcgagac tgaagtaacc
      301 tctcgtcccg accgtgcctt cttgctcaaa agcaaactgc attcccaata catacagaat
      361 aactacgatg tggttttaat cgatactcag ggtgcagttg gtcctctctt agataccgca
      421 gcatttgctg ctacatgcct gctttgccca attatgcccg aagttttgag tgcccgtgag
      481 ttccttaccg gtacgcaaga acttctaaag cgtctgcaaa aaggcgaagc aatgcgtatc
      541 ccgatgcctc aattacgagc catcatctac gcacaagatc gtaccaatga tgcgaaattc
      601 atcgcatctt ctatcaaaga tttctttagc aatactctag atgacagacg taaattgtta
      661 agcgtagcag ttcctgctgc caaagcatat aaggaagcta caactttaag agtcccggtt
      721 cattgtcatg agcgtaaaca gcttggaaag atgctgcctg cttatgaaat catgcaccgt
      781 attgtttatg agattttccc tggtatcgaa cagaagaacc tgcgtggaag ctgtttcaat
      841 gacatggcca gcctgctgca aaaggaggtg caatcatgaa tttggaccaa aataaagcaa
      901 ccttgagcct gcacatcggt acccctgcac agacaaatat gagttttggc caagaggata
      961 ctccttctcg gggcatgtta atgtcgttgc caatcagcga aatcgatttc tttgacaaaa
     1021 accctcgccg tttacatgat gtttccagct acgcggccat caaagagtcc atccgttctt
     1081 caggtattca acaacctgta cacgttaccc agagacctgg tgaaagtaaa tatgtactgg
     1141 cacagggtgg taatacccgt ctcaaaatct tgtgcgaact cttggaagaa actggtgata
     1201 accgcttccg tatgatgcct tgtatttaca tcccctactc tgacgaaact acattgcagg
     1261 ttgcgcatct tgttgaaaat gaactccgtg ctgaaatgtg tttttgggat aaggcctgta
     1321 aatatgccga aatccgcgat attttccaag atgaatctga ctcagggaaa ctttctttgc
     1381 gcgaactgga agtgatgttt tctgataaag gccttacggt ttctcataag actttaggat
     1441 tattcttttt tgcctccgat aatttacaag agctgcaaga atacgcattc aaactttccc
     1501 atgcaaaagt agaagaaatt aaaaaacttc actctaccct attctcaatt tctaaatcaa
     1561 ttcagttgga cgattctttc caatatttgt ggtcggatgt actggagcag tggcataacc
     1621 aatattctgc aacagagtta gacgtgcatg agctttatca gttcgtgctc gacagattta
     1681 aatttgaata tggctctgac gccttttctg aaacagaaaa ttcttctgat aaagacaaaa
     1741 ttgaaacagc cgtcaaacaa gatgtcaatt tgcatcaaaa agaggtatcg gaaaacaaca
     1801 ctgagtcaaa ggattcggat atggaatttg tgccagccaa agtaaatggt actaaagctg
     1861 atgcagagga tgagccgaaa gaagccgaac cgattacttt ggagcaaacc agcaaaaaac
     1921 tgttttctgc tgttcgtaaa ctgttagcca aagtgagtct gtccgattgc tttaagattc
     1981 ataaggactt tccatacggc tggtatgtcg aatatccggc ttttgagcat atttcagcat
     2041 caagtgccgg aaatgccatc tatgctattg atgttttgca tgaagaagcc ggaaatgttt
     2101 tcacattctt atggaagctt agtggaatcg acacctttat gtttgatgtt ggtgatagtt
     2161 ccaatcccct gctgacactt aaaagtgatt caaagcttcg tattgcctac gaggaccccg
     2221 ataagtacga cgaatatacg aactgcggaa ttggtaataa agagaacatt attgcctctt
     2281 tactgaattg gcaaactaat accagccatg aatttcacgc caatatcgta gaaatattat
     2341 ctctgagcac acagtacaac aacttgagaa aaacgggagc taagagtgag gagtaagcct
     2401 ttaattagta atcccattta tagacttttt aatacggatc cggtttatac caatcttgca
     2461 cttacaaggt tggcatccgc accaaatacg ccgcgtgaat cttcttataa cgctttggtt
     2521 gctcgtgaac atgaggatgt tttgaacacg ctcaaacgaa tgaacccgaa gcggattgag
     2581 aaattagcat ccatgtgtcc tgatgttgtg acctactcca ttgatctgaa ggttttaaaa
     2641 agcaaaattt cagaaatgga acattacgaa caaaggttta tggaacagat gcagcgtctt
     2701 aaatggatgt tagaacataa ggccagcaat ctgatgatta tgaatctatg ttcattaatt
     2761 tctacggcgg aaattaaaaa attgcgtata gaaatgaata ttcctgtcgt taaagggcgc
     2821 attgtgatgc catctattga tgttcgcgtc agggtattga aggcatggag taagtcggaa
     2881 aaagagccag atttgtttat tagataccag ctattgattg atgaattccc cgattatagc
     2941 cttgcccaat tacattcaat aattaacgat gtaaagttct ttggagtatg agtatgtata
     3001 tggatttctc tacttcctat gattatgaga ctggttttgt accatctaag aacatgtcaa
     3061 ccgataactt tatccgtagc ttggttgcct tgtgggagga accggccaca tatgtgcgtc
     3121 agtttgctca ggcggtcggt tctgtttcag agggatattt tttgagttgg tttgtcgaga
     3181 aatgtctgcc tggcgaatgg gtaagcctga ataatgatct gttgtttaat gaagcaggta
     3241 ttaccgcaaa gcagtggcgg aatattcgca atagccttct gcagaaaggt attttgctta
     3301 atcggagaac cgtagcgcca accgaatcac aattcacatt gaatgatgct ctttttgagg
     3361 atttggtaag taaacatggt aaatcagatg tacttgacat ggtttcgccc cctatttcga
     3421 ttaataaact tcacctgaag acattagccg atagaggtct tgcttttgta tccattttgt
     3481 atttgtcgtt tcttcaaagt gaagctgaat atcaagattt gagtaagcgt ggtgactggt
     3541 ctccttggac tcatgcagct gaaaagcacg ttttttattc aacaatgttg tcaagacggc
     3601 aacaggaaag agctattgcc gaattaacct cttttggttt gattgagagc tgtgttgatg
     3661 gtatgccagc agcgcgacgt tgtagataca gcttgaaaat gcttgctgat cttacttctg
     3721 aatatttgaa cggatgtgtg tgatggttac cgccaccaaa ttaccccctc ccaaagtatt
     3781 taactctgaa ctgacacaat gctggcaagc attgcaaggt ggtcgaaggg ttgccatcta
     3841 tcgatgttta attgatatta caggctctct gaagactgct gcaatgcttt ctcagttaat
     3901 ctattggaca agagtaagca aagaggttgc cgaacgggat ggttggattt tcaagtctat
     3961 tgctcagatg gaagctgaaa ctggtttgac agtgagagag cagcgcactt gtaaaaacaa
     4021 gcttcttgag acaaacctta ttcaaacatg ccgtaaaggt gtgggggctg ctttagcaat
     4081 caaagtgaac ttggatgcca tagctgagtt gatcgctaaa tcgagcggta ctgacgatca
     4141 acttgcactt acgctggctg atttacagaa cacaagttcc ctgtatttta gaaaacactt
     4201 ttctaaaagg attgcttatc accgagacct ggtttctatt acaggctgta tcaactcggc
     4261 tgtattattg tcgtgtgtgt taaatgatgc ggttggtttg gtttctcaaa atccacattt
     4321 acaacgtcat gcctttgcta ctttgactgc tgctgattgg gaggaaagaa cctctctctc
     4381 ttataaatct caattgacag ctcgcaatag gctgaagaat ttaaatctca tttatgagag
     4441 aaatttcttg gccagccgcc gtatttttac cctggtaaat ggtcacgata ttgttatatc
     4501 catcaaaaaa cttctagctg gcaaacagga cgacggcttt agtccatata actccaaaaa
     4561 acgcgaaatt ctcagtttgg cggaaagggc caaatgtgat tggcggaaag ggccaaatgg
     4621 attggataaa gggtttcaga gcggtttcga gggtgatgcc cccctattcc caagtatgac
     4681 atccggaagt gtcgaaaggg aatattcgga tgtgtcgaat tggaatttta agaaaggtcg
     4741 tagtgggaaa ttcataagtg tcgattgggg aagttcagaa ttgccgaatc ggcagattat
     4801 ttattatata aattacaaag gggtttataa ctacaactac atgactgttg aacccaacaa
     4861 accacgttct tccgaacaag atcatgtggt agtggttgat gatgttgatt tgatttatcc
     4921 aaaacttttt aatgtgcaaa tgcgtcaaca ggcggatttg atttttacaa aacatctctc
     4981 aaaaagttcc tgggctttga ggcaggaaat cttggatgag atccagggac agaaaaaaga
     5041 agttactagc ccccttggtc ttttgattaa cctatgccgc tgcgcttcgc aaggtaactt
     5101 tactgctctt gtcgccccac aggttagatt ccagcgtgag caggatcaga aattacaggc
     5161 ttatcttgaa aagcagaata accaaaaaca ggactcatta gatgccatg
//
LOCUS       DAXFWM010000099         5155 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000099 DAXFWM010000000
VERSION     DAXFWM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.099"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(27..1013)
                     /locus_tag="ACFI8B_002041"
     CDS             complement(27..1013)
                     /locus_tag="ACFI8B_002041"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC3835164.1"
                     /translation="MPNDLVVVKANSLIEANYRLSIDEIRILALTIGTMDPKSNQKIF
                     DFTVADFVREFPEISQDNAYKQIQAAIKRIYDRSVKTEDKDRVTEFRWVSSRTYFKKE
                     GRFRIAMTDEVMPYLTQLKGQFTQYQLKHIAYFNSVHSIRIYELITQYRSVGSREITV
                     EKLKEWLQVENKYPRFNSLNQRVLEPAITEINEKSDLVVEVEQIKRGRTIHSLNFVIG
                     SKKRTAQKIEEVAKRPVFPHKNKYGKFVKLDKQNPKMSNHEYGLWARDCLKILEDHYT
                     DITKVTNEDLRNYWVFLAGNDSNRSKLGSKSDFLNELKKRGYKLVDCELVKI"
     gene            complement(1413..1670)
                     /locus_tag="ACFI8B_002042"
     CDS             complement(1413..1670)
                     /locus_tag="ACFI8B_002042"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835165.1"
                     /translation="MSEIVTIIFYNVLILSFIAISVYVYLKWQHKKRIDRMLDEMEKS
                     YSKFADHKANERMQKRIDEYDKRHPLKAFKRKFKDKIKWLL"
     gene            complement(1885..2904)
                     /locus_tag="ACFI8B_002043"
     CDS             complement(1885..2904)
                     /locus_tag="ACFI8B_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFC3835166.1"
                     /translation="MGQSLFYVHLDDMTNDLTVHKANNFVQASYSMTLDEMRILALTL
                     GVFNPKNPSKRGFDFTVADFCKSFPDVNPDIAYTQVRNAVLKISKRWVTLVDNEHELT
                     EVALIHKRSYFKKEGRFYIEFHDELIPYISELHDNYTKYKLINIGALGSTHAIRLYEL
                     CSQYRDTGWRQTSVEDIKSWLSISDKYPLFKDFKKRVLTPSINEINAKSDLLVDVEPI
                     KRGRTIVALKFTIKSKKSAVKIEQKRPAFPHKNKYGKFVKLDTQNPKMSNAEYGNYAR
                     DCLKILEDFYSDLADVTTEDLRHYWVFLTSNASFRSKLGKRSDFLNELQNRGYKIVNC
                     ELVKV"
     gene            3307..3837
                     /locus_tag="ACFI8B_002044"
     CDS             3307..3837
                     /locus_tag="ACFI8B_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC3835167.1"
                     /translation="MSVYLVEAQPAKVIAPTRTAINRIFTDKASGSSTDREGLDLLRM
                     KVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTIL
                     SAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLS
                     IARSTVYKILEDERTS"
     gene            4020..4880
                     /locus_tag="ACFI8B_002045"
     CDS             4020..4880
                     /locus_tag="ACFI8B_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-135"
                     /protein_id="HFC3835168.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTTPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(4975..>5155)
                     /locus_tag="ACFI8B_002046"
     CDS             complement(4975..>5155)
                     /locus_tag="ACFI8B_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HFC3835169.1"
                     /translation="KKNKMNSNFITETEKLSREKAVRAAIDNNRLEGLEPSQEFIDRG
                     LTLSGTKTHVKGFWS"
BASE COUNT         1533 a         1035 c          943 g         1644 t
ORIGIN      
        1 aaaaaaaagt taagtaaaat acctacctaa atttttacta gttcgcaatc tacgagctta
       61 taacctcgtt ttttcaattc atttaaaaaa tcagattttg agcctaattt tgatctattg
      121 ctatcgttac ccgctagaaa tacccagtaa ttacgcaaat cttcattggt aactttcgta
      181 atatcggtgt aatgatcttc gagtattttt aagcaatctc tagcccataa accgtactcg
      241 tgattgctca tcttagggtt ttgcttatcg agtttgacga acttcccata cttgttttta
      301 tgtggaaata ctggccgttt tgcaacttct tcaatttttt gagctgttcg ttttttacta
      361 ccaatcacaa aatttaaaga gtgaatagta cgcccacgct tgatttgttc aacctcaacg
      421 actaaatcag atttctcgtt aatctcagtt attgcaggtt ccaaaacacg ttgatttaat
      481 gaattaaatc tagggtattt attttcaacc tgaagccatt cttttagttt ttctactgta
      541 atttcacgac taccaacaga gcgatattgt gtaattagct cataaattcg aattgaatgt
      601 acactgttga aataagcgat atgtttgagt tgatattgcg tgaattgccc tttaagttgc
      661 gttaggtatg gcataacttc atcagtcatt gcaattctaa aacgcccctc tttcttgaaa
      721 tatgttctag aggaaaccca acgaaattca gttacacggt ctttatcttc agttttaaca
      781 cttcggtcat aaatccgttt tatagccgcc tgaatttgct tataggcgtt atcttggctt
      841 atttctggaa actcacggac aaaatcagcc accgtaaaat caaaaatctt ttgattagat
      901 ttcggatcca tagtcccaat agttaaagct aaaattctga tttcatcaat actcaatcgg
      961 taattggctt caataaggct attagccttt acaacaacta aatcatttgg cataagacaa
     1021 caaatttcct gtttaaaaca acaagcaaaa tatacctgtt gtttatatat aaaacaacaa
     1081 gtattttctt aaaagttgtc tataacagga aatttgttgt cgtataacag gaaatttgtt
     1141 gtcgtataac aggaaatttg ttgtcttata acaggaaatt tgttgtcgta taagtttgta
     1201 acttattgat tttactggtt ttaaaaacgc cgaaaacaag taaaaaacaa aaatataaaa
     1261 atatagggac tttcgtccct tttttgggct ttcagcccta attttttctt ttttcaggat
     1321 taaaaattac aaaaccctta cagagcaagt aaacttgttt gcttgttctg caagggttca
     1381 gcaaccgaag ccgttaggcg tagggcggta gcctataaaa gccatttaat tttatcttta
     1441 aatttccgtt taaatgcttt gagtgggtgt cttttatcgt actcatcaat ccttttttgc
     1501 attctttcgt ttgctttgtg atcggcaaat tttgaataag atttttccat ctcatctaac
     1561 attctatcaa tccgtttttt atgttgccat ttcaggtaaa cataaacact tatagcaata
     1621 aaagacaata tcaatacatt gtaaaaaatg attgttacaa tttcgctcac agttattttt
     1681 tacctttttc aatttcttca ttgataaatg cactcaattc atcaaatttc ttgtcatcat
     1741 tgataaattt acgcaactta gggaagtttc tatctacatc taaaagaggg ttaaacgatt
     1801 attatcaata gccgctctaa ccgctttttc tcggcttaat ttttctgtct ctgttataaa
     1861 attgctattc atcttgttct tctctcacac tttaactaat tcacagttca caatcttata
     1921 ccctcgattt tgcaactcgt ttaaaaaatc tgaccgctta ccaagttttg atctaaaact
     1981 agcattgcta gtcaaaaaaa cccaataatg ccgtaaatct tctgttgtaa catcggcaag
     2041 atcagaataa aaatcttcaa ggatttttag gcaatctctc gcatagtttc catattcagc
     2101 attactcatt ttgggatttt gagtatccaa tttcacaaac ttcccgtact tgtttttatg
     2161 cggaaatgca gggcgtttct gttcgatttt taccgcactt ttcttactct tgatcgtgaa
     2221 ttttaatgct acgattgttc gcccacgctt gataggttca acatcaacaa gcagatcgga
     2281 tttagcatta atttcattta tggatggagt taatactcgc tttttaaaat ccttaaacag
     2341 tgggtactta tcagagatac ttaaccaact tttaatatct tctacgcttg tttgtcgcca
     2401 acctgtatca cgatattgag aacacaattc ataaaggcga atagcgtgcg tactacccaa
     2461 agccccaata ttgatcaatt tatattttgt gtagttatcg tgtaattcag aaatgtaagg
     2521 aattagctca tcgtggaact cgatataaaa tcgcccttct tttttaaaat aggaacgctt
     2581 atgaattaaa gctacttctg ttaattcgtg ttcgttatca accagtgtaa cccaacgctt
     2641 tgagattttt aaaacggcat ttctaacttg tgtgtaagct atatcaggat ttacatcggg
     2701 gaagctttta caaaaatctg ccaccgtgaa atcaaatcca cgcttagacg gatttttagg
     2761 attaaaaacc cccaaagtta aagccagaat ccgcatttca tcaagtgtca ttgaatagct
     2821 ggcttgtaca aaattgttag ctttatggac tgttaaatca tttgtcatat catcaaggtg
     2881 gacataaaat aaagattgtc ccattataac catacagtta aatggtggtc aataaaaaac
     2941 aaagaccact ataacaataa atttgtccac ctataacaat aaatttgtcc acctataaca
     3001 ataaatttgt ccacctataa atctcgcaag ccttgtgtaa caaggggagc cagagcctac
     3061 aaacaagaat acaaacaaga atacaaaaaa atagagccta aaggctcttt ttggggcttt
     3121 cagccctaat tttttctttt tttcaggatt taaaattaca aaacccttac agagcaagta
     3181 aacttgtttg cttgttctgc aagggttcgc aataaaaaca accgctaaac atttctgccc
     3241 agcggttgaa aatttaccta ttcaccatta caatgatcaa gcaggaaatt tttttgattg
     3301 ccgtaaatgt ccgtatatct agttgaggca caacccgcca aagtcattgc cccaaccaga
     3361 acggcgataa accgtatatt taccgataag gcatccggca gttcaacaga ccgggaaggg
     3421 ctggatttgc tgaggatgaa ggtggaggaa ggtgatgtca ttctggttaa gaaactcgac
     3481 cgtcttggcc gcgacactgc cgatatgatc caactgataa aggaatttga cgctcagggc
     3541 gtggcagtcc ggttcattga tgacgggatc agtaccgacg gtgatatggg gcaaatggtg
     3601 gtcaccatcc tgtcggctgt ggcacaggct gaacgccgga ggatcctaga acgcacgaat
     3661 gagggccgac aggaagcaaa gctgaaagga atcaaatttg gccgcaggcg taccgtggac
     3721 aggaacgtcg tgctgacgct tcatcagaag ggcactggtg caacggaaat tgctcatcag
     3781 ctcagtattg cccgctccac ggtttataaa attcttgaag acgaaaggac ctcgtgatac
     3841 gcttattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     3901 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3961 atccgctcat gagacaataa ccctggtaaa tgcttcaata atattgaaaa aggaagagta
     4021 tgagtattca acattttcgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4141 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4201 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggtgcg gtattatccc
     4261 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4321 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4381 gcagtgctgc cataaccatg agtgataaca ctgctgccaa cttacttctg acaacgatcg
     4441 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4501 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgacgc
     4561 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4621 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4681 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4861 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agacccctat ctataaactc
     5041 ttggcttggt tctaatccct ctaaacgatt attatcaata gccgctctaa ccgctttttc
     5101 tcggcttaat ttttctgtct ctgttataaa attgctattc atcttgttct tcttc
//
LOCUS       DAXFWM010000100         4636 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000100 DAXFWM010000000
VERSION     DAXFWM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.100"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(318..677)
                     /locus_tag="ACFI8B_002047"
     CDS             complement(318..677)
                     /locus_tag="ACFI8B_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835170.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RVARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(798..1884)
                     /locus_tag="ACFI8B_002048"
                     /pseudo
     CDS             complement(798..1884)
                     /locus_tag="ACFI8B_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
     gene            complement(1894..2184)
                     /locus_tag="ACFI8B_002049"
     CDS             complement(1894..2184)
                     /locus_tag="ACFI8B_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC3835171.1"
                     /translation="MPLLSGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLISGFGQGLGCLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(2198..3754)
                     /locus_tag="ACFI8B_002050"
                     /pseudo
     CDS             complement(2198..3754)
                     /locus_tag="ACFI8B_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
     gene            complement(3696..4013)
                     /locus_tag="ACFI8B_002051"
     CDS             complement(3696..4013)
                     /locus_tag="ACFI8B_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC3835172.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(4141..4419)
                     /locus_tag="ACFI8B_002052"
     CDS             complement(4141..4419)
                     /locus_tag="ACFI8B_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835173.1"
                     /translation="MYCQVGNKCLEKHRAENLYFSLVVPRIKENGQIIRPEYNGSMWK
                     MSDGQPLRLSLAECSPKDNLQSGLETGRIVFGVLASVYFVSLLKKVLK"
     gene            complement(4426..4636)
                     /locus_tag="ACFI8B_002053"
                     /pseudo
     CDS             complement(4426..4636)
                     /locus_tag="ACFI8B_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
BASE COUNT         1117 a         1134 c         1044 g         1341 t
ORIGIN      
        1 tgcgacccga atgtctgatt ttttttgggc gtatctcagt ccggaatcac tccgttagtg
       61 ggtttgcggt attgaaaaac agttcataaa aaaggaaaag ggggtattcg taaagatttg
      121 gcaaaaagcg cgcccaatct ttacaaagct tccccctttt ccttttttcc gccctatttt
      181 cctgcaccta caacccccga acgaagcgat tccggactga gacactttaa aaaaaacagc
      241 cattctagca gttaaccccc ttcgctccgc ccaagccatc ctgaggtgta gtggctgaat
      301 ttgtgatttt ggttttatca aacaaaatat ttgactgaag tcacatggcg gtcgtcatat
      361 gggggttcct tcgcacccaa aaaatcgacc gcgtaacggt ttactaaaac ttcatcgtcc
      421 ttaaactttt ggtgcttttt ccggcaatat tttctgaact ccgttaaatt tgacggcaag
      481 aacccgcaac cgtctgcacc gtaaatgtaa tcaacttcaa acaaactgtc tttccgggca
      541 acccgaccat ctttaagcca aaacataggc tcgaaataaa aaactttcga cgtgtccggg
      601 aacttatgga gacggagaac acgtgtgaaa tccccgtttt catctacaat tttgatatat
      661 cttggtttgt gaatcatgac atcctcagat ttagtattca gaatatgatt ttaaaaagaa
      721 ctttctgctt tacgactccg ccgccgattc cttcaaacgg ttttccgcgc tcttcagttg
      781 tcgtacatga gattttgcta cggctttccg cccattacga gaacttgggg cttgtccgct
      841 ttcgcggact gtgccgcctg ttccgtcctt tgccgttcgt ccttgtaagg attgaaaggc
      901 aacccgtttt tcacatattc tttacacatt atctttgtta tttctttcaa gggtgttcct
      961 tgatttgaat agcatgtgca atctgatttt ccgccgtcta tgcatccggc gatttgctca
     1021 aaggttttta cttgtcggac tgtgttataa ataggcttgc tttcgggctt ttcgggcaaa
     1081 gtcggcacaa agtcttcagg tttcagattg tcggaatgct caaaaggcgc tgtttctgat
     1141 gatgccgtct gctccgtcat cgtctgcaca acgctttctt tttgcgcttc ctgctcaatc
     1201 cggctgtctg tggctttgct gtaaacttga aacatgccgt aacttttcca gcctacaaac
     1261 cctacaaccg caatcaacgc ccaaaccgcc caaggcactt ttttcttgaa cttttggtgc
     1321 cggcttgcgg atttatagta tttgaaggct tctttaggcg gtttccaatt tgcggcttct
     1381 acgccgctca cgcctgccgg attgtccaac gaggttacgc atttatacca ataatactgt
     1441 ttcatgccga ttgccttgcg ttcaaggtgt acatgctttg aaacaaggtt gcggacgaat
     1501 atatcaagtt ggctcgggtg ccgcgtcatc aaaatgacgg tatgcccgtg atggcggagt
     1561 tctgtcagtt cctgaatata gggcggaacg ggacggcctg ccgcgcgtac cgggtaagtg
     1621 tagtgcgctt cgccaacaat cagcaccgcg ccttccggta tgacatcacg aagcggggcg
     1681 gacatgattt gcccttccgc cagttcgcgg gcattgaatt ttcgtttgtc caatccgtcg
     1741 atatggcaga aataaagcgg ccggtctgcc tccgtgccgt cttccaattc cattttgaac
     1801 aatccgtctt cgttgttcaa aatcatagag acgacgcggg ggggttttgc ctgcccccat
     1861 gtttcccgta aacagataaa tcatgtttct acctcatccc ggaaagacaa acgtcagttt
     1921 tttgaatgcg tgcataccaa tgaagaacga gaatgcgccg aacaggcagc ccaacccctg
     1981 accgaatccc gaaattaaaa gaaggttcaa tatgtcggaa ggcatggaat tgatcgcatt
     2041 tgccgtgtag cctttgaact tttccagtgc ggcgagatac ccggcatagg ttacgaatgt
     2101 cagacctgtt gcaaggatga ttctgacaat cagcattttc agaagtatgc ctaaaagtgg
     2161 aatcaggccg gaaagtaatg gcatttattc ccccccccaa cgaaccgaaa acgacaaaag
     2221 ccgacataat gataaaggca agcagtacgg caaaacggat tttttcggca aacacgcaca
     2281 acggttcata gcttgcccga tattgcctgc cgaaaacatg aaaggttttc ggctgcggac
     2341 atacgccgtt agacggtaaa aagttatgtg aagaccatgt tttatcgtct gtaacctgcg
     2401 gtatgcttat atcgtgaaac atgcggtccg aaggtttgcc catctcctga caggctagga
     2461 tttccggaaa ataatcgcac gaaagcccgc cgtcttcgcc ttctttcctt tctttgcgat
     2521 gcctgccgtt tgggcggtcc ggaacggccg gggaatcggg gcttgttccg ggctgtccgt
     2581 ccgtatcggg atttgcatcg ggattcaaat cggggtcggg ttcgggattg ggacgcgtgc
     2641 cggggttctc atcggggtcc gggttgtttg cggggttttc ggcgggcgat acttcgggcg
     2701 gcggctgtgc gtgaggtgct tccgcgcttc caggggttaa atcgggacgc gggattactt
     2761 gtacatccgc cgtggtgttg ccttgcgcgt ccctgccgaa tgttgcggca acctgaacgg
     2821 gattcccgtt cctgtccgtg acgggcccca tattcacttt tgttccgggt gcgacttcta
     2881 ctttttcgga ataaccggga tatccggttg cctctatgta tttgtcggga tcggcatcga
     2941 ctttcaacga taaaatctct tccggctttt tggcatccat ttcttctttg tattttggat
     3001 tgcggccaag ggaaaaagaa gccccacctc tgaaatcatc acctttattg accgcacaac
     3061 ctccgccgtt ccaatcaaat gtgcaacgat ttaaaacaaa attattccaa tccaaagaac
     3121 ttaatttatt cagttcttct ttacgccaat tccaaaacgg acgtgccagc ctatacattt
     3181 ggctttccat cagttgtttg acttcgggga atctgctgcg atcgggcata aggcgcataa
     3241 tcgaactgtc aacgccgtag cagccatagg ttctattaat acgtctttcg tcttcgtacc
     3301 aaaggcaatt agcatattcg tagcctttta caaatttgtc ggtttcggga tcgtatcggc
     3361 agcctcgtgc ctgtatgtct tctttgaaag tttggtatac gtcgtgggct aaaagggttg
     3421 ttccgacata gggaaccgcc cttgtgccga atttcgcgcc ttggcggaca agtttgccga
     3481 cccccgacaa tacgccggcg cgggatacgc tggcggttat tttggcgttg attcgggctt
     3541 ttgcgcccgt ggggatgtgc tcgaaatttg ccgcttctgt gaatttggaa aaactgttag
     3601 aacttgaatc taatttaaaa ccaattgatt ttaaattatt acttgattga aatttaattt
     3661 gtgatttttc taatcttaca ggttcagaat aagaattact cgaacataga attaaaatca
     3721 gaactatcgg tgtactcttg ataaacgaat tcatgaacag tttttccgtt ctcttctgat
     3781 tttcttataa aaataacaga ctcatcagaa aaataaatct tccaaatatt atgtgcgacc
     3841 ctcttattta agaaataaga gaagctctct aaaatatcgt actcttttac gttattaaca
     3901 aattcttcaa attctttctg agcaataaaa gccatcgatt gcccaaaata cttgcttgac
     3961 ggctgatatt tataaagtgc caactgcgcc tgcgtgataa acggcttgtt catggttctg
     4021 cctttcaaag gttgttttga aagcctgatt ttgacaccat aacttcatgc gctcaatcct
     4081 taaacagaac cgccccgatt aatacgggga cggcaacgcc gagatagaaa taaaaatcca
     4141 tcatttcaaa acctttttca gcagggaaac aaagtaaacg gacgcgagga cgccgaatac
     4201 tatccggcct gtttcaagac cgctttgcag gttgtctttc ggactgcatt ccgccaatga
     4261 aagccttagc ggctgaccgt ccgacatctt ccacatgctg ccgttatatt ccggcctgat
     4321 tatctgtccg ttttctttga ttcttggtac taccaagctg aaataaaggt tttcggcccg
     4381 gtgcttctca agacatttat ttccgacttg gcagtacatg ccgccttact tcatcaccct
     4441 cttaacgatg gaaaatacaa aaagcgcggc gaaaacgccc actacaatcc aaccggcttc
     4501 cataccgtcc gcttttgcgg cttccaaagc gttttttgcc gtttcgggca acgctgcgtt
     4561 tgcctgtgcc gccaaagcca gcgggggcgg ctgttacaac agccagtttt gcgccgtatt
     4621 tacggcaggt gttaat
//
LOCUS       DAXFWM010000101         4256 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000101 DAXFWM010000000
VERSION     DAXFWM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.101"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..200
                     /locus_tag="ACFI8B_002054"
                     /pseudo
     CDS             1..200
                     /locus_tag="ACFI8B_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(198..390)
                     /locus_tag="ACFI8B_002055"
                     /pseudo
     CDS             complement(198..390)
                     /locus_tag="ACFI8B_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            784..2121
                     /gene="ribBA"
                     /locus_tag="ACFI8B_002056"
     CDS             784..2121
                     /gene="ribBA"
                     /locus_tag="ACFI8B_002056"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC3835174.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQTNPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2284..2400)
                     /locus_tag="ACFI8B_002057"
     CDS             complement(2284..2400)
                     /locus_tag="ACFI8B_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC3835175.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(2432..3508)
                     /locus_tag="ACFI8B_002058"
     CDS             complement(2432..3508)
                     /locus_tag="ACFI8B_002058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC3835176.1"
                     /translation="MIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVDV
                     YDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQGN
                     TILSPYHKDASDRLKKFDFIVSNPPFKLDFSDFRDQLESDENRERFFAGIPKIKAKDK
                     DKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSGIDKKIREYLVENKMLAGVVSM
                     PSNIFATTGTNVSILFIDKTNKDKVVLIDASGLGEKIKDGKNQKTVLSCEEEQKICNT
                     FTNKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFAQKIAGFSADLD
                     KLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(3512..3652)
                     /locus_tag="ACFI8B_002059"
     CDS             complement(3512..3652)
                     /locus_tag="ACFI8B_002059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835177.1"
                     /translation="MFERISQYITDEGRRDDFCRALISKLAGFSFEAIFAQKFDFFAT
                     IF"
     gene            complement(3671..4033)
                     /locus_tag="ACFI8B_002060"
     CDS             complement(3671..4033)
                     /locus_tag="ACFI8B_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835178.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEAIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKT"
BASE COUNT         1079 a         1149 c          936 g         1092 t
ORIGIN      
        1 gcgttaataa aaataccgca gcctatgatt ttcatcgttt acgatgactg atttatcaaa
       61 acggtccgca tttagaaatg tttgatggcg aagtagaagc agatgaaagt tattttggcg
      121 gacaacgcaa aggtaaacgc ggtcgcggtg ctgccggaaa agtcgcagta ttcaggcttc
      181 tcaaacgaaa tggcaaggtt tcctttgccg tccgttatgt acgtttcgta acattcgaag
      241 acttcctgaa ccctgcccgc cgtttcggtt tctgtacccc ctgttagata aggggggaag
      301 attcgaagcg gttgccggct tcctgccgtc cgccggcgcg tccgtcatca cgccggcaac
      361 cgcctttttc atcccttgct tatcttccat ttgcgaatcc tcaaaaacgg gcaaaaaaaa
      421 gccctgttac ttgtagaaag taaaaggcga tccgtccggt ttttatcggg aaggcggcat
      481 tcatcatagg attgccggta ttgcgcctgc tcgtaccgcc tatgccgtac ccggcaatca
      541 aggcttttta tcagaatttt tttcggaata accctccgga aaccccataa tcagaagcgt
      601 gcgggaagaa acagccgccc cgccggcggg gttgcggcaa tgccgtctga agccacgaat
      661 ccggcttcaa acggcatctg tttaccaaaa ggcaaataat ccgatttggc gaaaaacaga
      721 tttgcttttt ggtaaatacg cgcttacaat ccgctacatc cgatttctac aaaggataaa
      781 acgatgaccg acacagccgg cttgcgccgc cgcaacctgc ggcaatggat agcggaacac
      841 tacggcggac tgcaaaaccg ttttgccgaa gccgttgccc tcaacacagg cgaactctcc
      901 gcccttttga aaaacaaatc cttcggcgag aaaaaagccc gcaaaatcga acaggcggca
      961 aaaatgcccg ccttctggct cgataccgaa cacaccggcc gaccgcccgg acacacagga
     1021 aaacacacca tgtcccatat ttcccccatc cccgaaatcc tagccgacat caaagccggt
     1081 aaaatggtca tcatcaccga tgccgaagac cgcgaaaacg aaggcgacct gctgatggcg
     1141 gcgcaattcg ttacccccga agccgtcaac tttatgatca aacacgcgcg cggcttagtc
     1201 tgcctgccta tggaaggtgc aatggtcgaa aaactcggtc tgccgatgat gacccaaaaa
     1261 aacggcgcgc aatacggtac caactttacc gtttccatcg aagccgcgca cggcattacc
     1321 accggcattt ccgccgccga ccgcgccctg actattcaaa ccgccgtttc cccgaccgcc
     1381 aaacccgcag acatcgtcca acccggtcat atcttcccgc ttcgcgccca aaaaggcggc
     1441 gtactcgtcc gtgccggaca caccgaagcc ggcgtcgact tggcgcaaat gaacgggctg
     1501 attcccgccg ccgtcatctg cgaaatcatc aacgacgacg gcacgatggc gcgtatgccc
     1561 gaactgatga aattcgccga agaacacaag ctcaaaatcg gcacgattgc cgacctcatc
     1621 gaataccgca gccgcaccga aagcctgctc gaagatatgg gcgacgcgcc cgtacaaact
     1681 ccgtggggcg agttccaaca acacgtttac gtcgacaaac tctccggcga aacccacctc
     1741 gccctcgtca aaggcacacc ctccgccgac accgaaaccc tcgtccgcgt ccacgaaccc
     1801 ttcagcgtga tggacttcat ccaaaccaat ccgcgccatt cctggtcgct gcccaaagcc
     1861 cttgagcgcg tccaacaggc cgaaagcggc gtcgtcatcc tcctgcacca caccgaagac
     1921 ggcgcatccc tgctcgaccg caccctaccc aaaggcgcaa accaagccta caaatgggac
     1981 agcaaaagct acggcatcgg cgcgcaaatc ctcgccggcc tccacgtcaa aaaactgcgc
     2041 gtcctcggcc aaccgtcatc tttcaccggc ctcaccggct tcggcctcga agtcgtcggc
     2101 tttgaagaag cggaaaaata atatagtaaa ttcaaatgct ttatatttgc ttcatttatt
     2161 gcattatttc cgtacaaacg aaaacccggt ctgttgggtt ggattttgct tttttcggat
     2221 ttcgggtaac ttctaattcg tcattcccgc gcaggcggga atccggttcg tcgggttttt
     2281 gtgtcatttc cgatgaattc ctgtggcttt ggttttttgg attctctctt tcggggaaag
     2341 aacggcataa gtattttcca aaccaaacaa aatgccgtct gaaaggcttt cagacggcat
     2401 tttaagtttg accggtttca ttcatcggta tttatgaatt gaatttcaac atcgccaatc
     2461 tatccttaat ctccttttcc aattcggcag attcggcgaa aagtttatcc aaatccgctg
     2521 aaaatcccgc tattttttgc gcaaattcgt cggcggatat atccacataa tcaattttta
     2581 cctcaaaata ctgccccgcc gacaagctgt gattcttcgc tttgatttca tcgtagccga
     2641 ccaccacgct aaaatcttcc actgcctgtt tgttcgtgaa agtattgcag attttttgtt
     2701 cttcttcgca ggaaagtacg gttttttggt ttttgccgtc tttgattttt tcgcccaagc
     2761 ccgatgcgtc aattaatacc actttgtctt tattggtttt gtcgataaac aggatggaaa
     2821 cattggttcc cgtcgtggcg aaaatattgg aaggcatgga caccacgccg gcaagcattt
     2881 tgttttccac caaatattcg cggatttttt tgtcgatgcc cgattgggcg gtgataaaac
     2941 ccgttggcaa cacaatcgcc gctttgccgt tttctttcag gctaaacaag atatgctgga
     3001 taaaaagctg gtagatttcc atcttatcct tatccttcgc cttgattttc ggaatgcctg
     3061 caaaaaagcg ttcgcggttt tcatcgcttt ccagttgatc gcggaaatcg ctgaaatcca
     3121 atttaaacgg cggattggaa acgatgaaat caaatttttt cagacggtct gaagcgtctt
     3181 tgtgatacgg cgacaaaatc gtattgccct gaaccacatt attcagcgaa tgcaccagat
     3241 tgttcaaact cagattcagc cgcaacaaat tggacgattt ctgcgaaata tcctgagtat
     3301 aaatcatgca tttatcttcg ccgatgacat gggcgacatt catcaacagc gttcccgaac
     3361 cggccgacgg gtcgtaaacg tccacgctgc ggatttgccc gcgcacctcc tccggcacca
     3421 aaatatccgc cataatccgc gcaaccgcgt gcggcgtgta gtattcggca tatttgccgc
     3481 cggaattact gttgtagtct ttaatcaggt attaaaaaat cgtggcgaaa aagtcgaatt
     3541 tttgcgcaaa aatcgcctcg aagctgaaac ccgccagctt ggaaatcagc gcgcggcaaa
     3601 aatcgtcgcg tcggccttcg tcggtaatgt actgactgat acgctcgaac aacacgattt
     3661 tcgccccgcc ttaggttttc accgaaaaca aatccgcatt acaggcggca atatcggtga
     3721 gcgtatcgtc aaagagtttg gcaaaatccg cgccgttttg ccgctctgat aaatacttga
     3781 tggaatgttc gggctttaaa accgctgttt ttccctcaat atccatattg atgaactcaa
     3841 tcgcctcgtc agggttttct ttgcggattt gtttcacttc gaaatcgtat ttgtcattta
     3901 ggaatttata caaaaacgcc tgactgatga ttttaaattc gttgccatcg ttacctaagc
     3961 catagttggc gcaaatggtt tttaagctgt cgattaggga tttgatttgt tcggtaaagt
     4021 gttgttcggt catggaatgt ctcggtttgt tgctagcgtc gcttaaattt tgtttagcat
     4081 tagttggttt gctggcagat taaataggtt gaacactatt tgccaacctt ttctcttttc
     4141 ctaatgctgt tgtgtaataa cgttcatcaa taccatataa caaatgtttc agctgtacat
     4201 cagtagaaat ttctacacga gagccatctg cgttaaattt caagatatta ttatta
//
LOCUS       DAXFWM010000102         4229 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000102 DAXFWM010000000
VERSION     DAXFWM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.102"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..248
                     /locus_tag="ACFI8B_002061"
     CDS             <1..248
                     /locus_tag="ACFI8B_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835179.1"
                     /translation="LKQIISKSDFFKIKFQKKETGKIRHKTLTYSKPSVLQRKYPKKT
                     ICLPSVSNRYTAGTRYFSDGAAVVRTDKNVTAAERIA"
     gene            302..2686
                     /gene="ppsA"
                     /locus_tag="ACFI8B_002062"
     CDS             302..2686
                     /gene="ppsA"
                     /locus_tag="ACFI8B_002062"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC3835180.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWDKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKASKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDTSEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2906..4165
                     /gene="rho"
                     /locus_tag="ACFI8B_002063"
     CDS             2906..4165
                     /gene="rho"
                     /locus_tag="ACFI8B_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC3835181.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
BASE COUNT         1113 a         1248 c         1005 g          863 t
ORIGIN      
        1 ttttaaaaca aattatctca aaatcagatt tctttaaaat aaaattccaa aaaaaagaaa
       61 caggaaaaat acggcataaa acattaacct attcaaaacc atctgtttta caaaggaaat
      121 acccgaaaaa aacaatatgc ctgccatcgg tttcaaaccg ctacacagcc ggaacccgct
      181 atttttcaga cggcgcagcg gttgtccgga cagataaaaa cgtgacagcg gcggaacgaa
      241 tcgcttaaaa taagcaagcg gatttgtttc ttttttaaca tattttggat tggacacaca
      301 aatggccgac aactacgtaa tctggtttga aaacctgcgt atgacagatg ttgaacgcgt
      361 gggcggtaaa aacgcctcgc tgggcgaaat gatcagtcaa ctgaccgaaa aaggcgttcg
      421 cgtccccggt ggctttgcca ccacagccga tgcctaccgc gcatttctcg cacacaacgg
      481 tctgaacgaa cgcatttccg ccgctctggc aaaattggat gtcgaagacg tttccgaact
      541 ggcacgcgtc ggcaaagaaa tccgccaatg gattttggac acgcccttcc ccgaacagct
      601 cgatgccgaa atcgaagcgg catgggacaa aatggttacc gatgccgacg gcgcggacat
      661 ttccgttgcc gtacgctctt ccgcaaccgc cgaagacctg cccgacgcat catttgccgg
      721 ccaacaggaa accttcctga atatcaatgg cttggataac gttaaaaaag cgatgcgcca
      781 cgttttcgcc tccctgtaca acgaccgcgc catttcctac cgcgtccaca aaggcttcga
      841 acacgacatc gtcgcccttt ccgccggcgt tcaacgcatg gtgcgttccg acagcggcgc
      901 atcaggtgtg atgttcaccc tcgacaccga atccggctac aaccaagtcg tctttgttac
      961 ctcctcttac ggtctgggcg aaaacgtcgt acaaggcgcg gtcaacccgg acgaatttta
     1021 tgtgttcaaa cccacgctca aagcaagcaa acccgccatc ctgcgcaaga ccatgggttc
     1081 gaaacacatc aaaatgattt tcaccgacaa agccgaagcc ggcaaatcgg taaccaacgt
     1141 cgaagtcccc gaagaagacc gcaaccgctt ctccattacc gacgaagaaa tcaccgagtt
     1201 ggcacactac gcgctgacca tcgaaaaaca ctacggccgc ccgatggata tcgaatgggg
     1261 acgcgacggc ctggacggca aactctacat cctgcaagcc cgccccgaaa ccgtcaaatc
     1321 ccaagaagaa ggcagccgca acctgcgccg ctacgccatc aacggcgaaa aaaccgtctt
     1381 gtgcgaaggt cgtgccatcg gtcaaaaagt cggtcagggc aaggtgcgcc tgattaaaga
     1441 tacttccgag atggattccg tcgaagccgg cgacgtactg gttaccgaca tgaccgatcc
     1501 ggattgggaa cccgtgatga aacgtgcttc cgccatcgtg accaaccgcg gcggacgcac
     1561 ctgccacgcc gccatcatcg cgcgcgaact gggtattcct gccgtcgtcg gctgcggcaa
     1621 tgcgaccgaa ctcttggaaa acggtcagga agttaccgta tcctgtgccg aaggcgatac
     1681 cggctttatc tatgccggcc tgctggacgt acaaattacc gatgtcgcct tggacaatat
     1741 gccaaaagca cccgtcaaag tcatgatgaa cgtcggcaat cccgaactcg cattcagctt
     1801 cgccaatctg cccagcgaag gcatcggctt ggcgcgtatg gaatttatca tcaaccgcca
     1861 aatcggcatc cacccgaaag ccttgttgga atttgacaaa caagacgacg aactgaaagc
     1921 ggaaattatc cgccgcatcg ccggttacgc atcccctgtc gacttctacg tcgataaaat
     1981 cgccgaaggc gtggcaacgc ttgccgcatc ggtttatccg cgtaaaacca tcgtccgtat
     2041 gtccgacttc aaatccaacg aatacgccaa cctggtcggc ggcagcgtat acgaaccgca
     2101 tgaagaaaac ccgatgttgg gcttccgtgg tgcggcgcgt tatgttgccg agagcttcaa
     2161 agactgtttc gccttggaat gcaaagcctt gaaacgcgtt cgtgacgaaa tgggtctgac
     2221 caacgtcgaa atcatgattc cgttcgtccg cactttgggc gaagccgaag cagtggttaa
     2281 ggcattgaaa gaaaacggct tggaacgcgg caaaaacggc ctgcgcctga ttatgatgtg
     2341 cgagctgccg agcaacgcgg tattggcgga acaattcctg caatacttcg acggcttctc
     2401 catcggctcg aacgacatga cccagctgac cctcggcctc gaccgagaca gcggcttggt
     2461 gtccgaatcg tttgacgaac gcaaccctgc cgtcaaagtg atgctgcatc ttgccatctc
     2521 cgcctgccgc aagcagaaca aatatgtcgg tatctgcggt caaggtccgt ccgaccatcc
     2581 ggactttgcc aaatggctgg ttggagaagg catcgaaagc gtgtctctga acccggatac
     2641 cgtcatcgaa acttggctgt atttggcgaa tgaattgaac aaataatcaa ttcccatacc
     2701 cccaagcctg aaaagcgcgg gggtattttt tccaagcagc tccgttcaga cggcatttcc
     2761 tgtcgatgcc ccgtccgcga taatatttga cacccacgcg ccgactgcct acaattcccc
     2821 ctctccgagc aaccggcaac agtcagcttc ttctttcaga cggcatccac ccgtcttttc
     2881 ctttctattc tatatatcat tgattatgca cgtctccgaa ttacaaaccc tgcacatttc
     2941 caaactctta gaattggcgg aagaacacgg catcgaaaac gccaaccgat tccgcaaaca
     3001 agacctcgta tttgccatcg tccgccagat gatgaaaaaa ggcgagggtt ttacctgctc
     3061 cggcacgctc gaaatcctgc ccgacggctt cggcttcctc cgcagcgcgg acacgtccta
     3121 tcttgccggc cccgacgaca tctatgtctc gcccactcaa atccgccgct tcaacctgca
     3181 tacgggcgac accatcgaag gcagcgtgcg cgtccccaaa gacaacgaac gctattttgc
     3241 cttggtcaga ctggattcca tcaatggcga ccacccggaa gtatgccgcc ataaaatcct
     3301 gtttgaaaac ctgaccccgc tgtttccgac cgaacagttg aagctggaac gcgacttaaa
     3361 gtccgaagaa aacctgaccg gacgcgccat cgacctgatt tcgcccatcg gcaaaggtca
     3421 gcgcgccctc ttggttgccc cgcccaaaat cggtaaaacc gtgatgctgc aaaacattgc
     3481 ccacgccgtt accgcaaact atcctgaagt cgaactcatc gtcctcttga ttgacgaacg
     3541 ccccgaagaa gtaaccgaaa tgagccgttc cgtccgaggt gaagtagtct cctccacctt
     3601 tgacgaaccg gcgcaacgcc acgtccaagt tgccgaaatg gtgcttgaaa aagccaagcg
     3661 tatggtggaa cacaaaaaag acgtggtcat cctgctggat tcgattaccc gccttgcccg
     3721 cgcctacaat accgtcgtcc ccgcctcggg caaaatcctg accggcggtg tcgatgccaa
     3781 cgcgctgcac cgccccaaac gtttcttcgg tgcggcgcgc aacgtggaag aaggcggttc
     3841 gctgaccatt atcgccaccg cattggttga aaccggcagc cgtatggacg atgtgattta
     3901 cgaagaattc aaaggtaccg gcaatatgga attgcacctc gaccgccgta tggcggaaaa
     3961 acgcctcttc cccgccatca acatcaacaa atccggcacg cgccgcgaag agctgcttgt
     4021 gccgaacgac cagttgcaac gtatgtggtt actgcgcaag ttcctgcacc cgatggacga
     4081 aatcgaagct accgagttct taaacggaaa aatcaaagca tctaaaaata atgatgattt
     4141 ctttgaactg atgcgcggaa aataaacgcg ccgcccgcat taatgcgaaa tgccgtctga
     4201 agcctgaaaa tcgggtttca gacgacatt
//
LOCUS       DAXFWM010000103         4206 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000103 DAXFWM010000000
VERSION     DAXFWM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.103"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..190
                     /locus_tag="ACFI8B_002064"
     CDS             <1..190
                     /locus_tag="ACFI8B_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835182.1"
                     /translation="GVKKGVHSATQNYRRSGAMPSERIFRSVLRLVGVSSDTVKRKFA
                     GAGLSYAGKAAGESLYCL"
     gene            306..1100
                     /locus_tag="ACFI8B_002065"
     CDS             306..1100
                     /locus_tag="ACFI8B_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC3835183.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            1325..3928
                     /gene="pepN"
                     /locus_tag="ACFI8B_002066"
     CDS             1325..3928
                     /gene="pepN"
                     /locus_tag="ACFI8B_002066"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC3835184.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLMT
                     EAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPETASWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
     gene            3990..>4206
                     /locus_tag="ACFI8B_002067"
     CDS             3990..>4206
                     /locus_tag="ACFI8B_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3835185.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVANHRLALAADEVFEGPAGPGGS"
BASE COUNT         1003 a         1166 c         1159 g          878 t
ORIGIN      
        1 aggtgtgaaa aaaggcgtac attccgctac acagaattac agacgcagcg gagcaatgcc
       61 gtctgaaagg attttccggt cagtcttgcg gttggtcggg gtttcatcgg atacggtgaa
      121 acgaaagttt gccggcgcag ggttgagcta cgcgggtaaa gccgcaggcg aaagcctgta
      181 ttgtttgtga agcgtaaatc tctgatttga ggtattgggg caatcctgtg ggggttgcct
      241 ctttttttat ccgcctttta atgacacaat aggcgcaagt ctgtttttga atgcaaggta
      301 ctgccatgaa tacgattttg gctttcgata ttgaaaccgt acccgatgtg cagggtatcc
      361 gtacgttgta tgacttgccg tcttccctgc cggatgacga agtggtgctg ttcgcgcagc
      421 agaaacgccg cgcacagacc ggcggcgatt ttatgcagca ccacctccat caggttgtgg
      481 cggtttcctg ctgtatgcgc tgggggcagg acaaggttca tgtcggcacc atcggcgaga
      541 tggacgatgg cgaagaagtc gtgattgcca agtttttcga attggttgaa aaacatacgc
      601 cgcaactggt cagctggaac ggcggcggtt tcgacctgcc cgtactgcat taccgctccc
      661 tgatatacgg catcaacgcc gcgcgctatt gggatatggg cgacggcgat ttcggcgaca
      721 gccgcgattt caagtggaac aactacatca gccgttatca ccaacgccac tgcgatttga
      781 tggatttgct cgcgctttac cagccgaggg cgaatgtgcc gctggacgat atggcgaaac
      841 tgtgcggctt tccgggcaag ctgggtatgg acggcagcaa ggtgtgggag gcgttccacg
      901 cgggcaggct gaaggaaatc cgcaattatt gcgaaaccga tgccgtgaat acgtatttga
      961 tgtatctgcg tttctgtctg gtcagcggca gattcgacgc ggacgaatac gaaatggaaa
     1021 tcaagcggat cagaaactat ctctccgccc aaacagaaga caaaccgcat tgggcagaat
     1081 ttgtccaagc gtggaaatag aaacctgctg tccgaaggcg gtacgcttgt gccgccgttt
     1141 cggtttcaga cggcgtatgg tggattaaat tcaaaccggt acagccttgc ctctccttgc
     1201 cttactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccgcttt
     1261 aaaagctgta tccgtgccgt ctgaagcctt ttccagtcaa accaaacaat attgaaagaa
     1321 aaaaatgagc aaaaccgtcc gttatctgaa agattaccaa acgcctgcct accgcattct
     1381 tgaaaccgaa ctgcatttcg acattgccga accgcaaacc gtcgtgaagt cgcgtttgac
     1441 ggtcgagccg cagagggcgg gcgagccgct ggtgttggac ggttcggcaa aactcttgtc
     1501 cgtcaaaatc aacggcgcgg cggcggatta tgtgttggaa ggcgagacgc tgacgattgc
     1561 agacgtaccg tccgaacgct tcaccgtcga agtggaaacc gaaatcctgc cggcggaaaa
     1621 caaatcgctg atggggctgt atgcttccgg cggcaatctg tttacccagt gcgagccgga
     1681 gggcttccgc aaaatcacgt tctacatcga ccgtccggat gtgatgtcca agttcacgac
     1741 caccatcgtc gcggacaaaa aacgctatcc cgttttgctt tccaacggca acaaaatcga
     1801 cggcggcgag ttttcagacg gccgccattg ggtgaaatgg gaagacccgt ttgccaaacc
     1861 gagttatctg tttgctttgg tcgcgggcga tttggcggta acggaagacc gtttcaccac
     1921 catgagcggc agaaacgtca aaatcgagtt ttacaccacc gaagcggaca aacccaaggt
     1981 cggctttgcc gtggaatcgt tgaaaaacgc gatgaagtgg gacgaaacgc gcttcgggtt
     2041 ggaatatgac ttggatattt tcatggtcgt cgccgtaggc gatttcaata tgggcgcgat
     2101 ggaaaacaag ggtttgaaca tttttaacac caagttcgtc ctcgccgaca gccgcaccgc
     2161 caccgatacc gatttcgaag gcattgaatc cgtggtcgga cacgaatatt tccacaactg
     2221 gacgggcaac cgcgtaacct gccgcgactg gttccagctt tcgctgaagg aagggctgac
     2281 cgtgttccgc gaccaagagt tttccggcga ccgcgccggc cgcgccgtgc gccgcatcga
     2341 gaacatccgc ctgctgcgcc agaaccagtt ccccgaagac gcaggcccga ccgcccatcc
     2401 ggtgcgcccc gtcagctatg aggagatgaa caatttctac accatgaccg tttatgaaaa
     2461 aggcgcggaa gtggtgcgga tgtatcatac cctgctcggc gaagagggct tccaaaaagg
     2521 catgaagcta tatttccaac gccacgacgg acaggcagtg acctgcgacg atttccgcgc
     2581 ggcgatggcg gatgcgaacg gcatcaatct cgaccagttc gccttgtggt acagccaggc
     2641 gggcacgccc gttttggaag ccgaaggccg tctgaaaaac aatgttttcg agttaaccat
     2701 taaacaaacc gtgccgccca cgcccgatat ggcggacaaa cagccgatga tgattcccgt
     2761 caaagtcggg cttctgaacc gcaacggcga agcggtggca ttcgattatc agggcaaacg
     2821 cgcaaccgaa gccgtgttgc tgatgaccga agccgaacag gccttcccgc tcgaaggtgt
     2881 aaccgaagcc gtcgttccct cgctgctgcg cgggttcagc gcgccggtgt atctgaacta
     2941 tccgtacagc gacgacgacc tgctgctcct gctcgcccac gacagcgacg ctttcacgtg
     3001 ctgggaagcc gcccaaacgc tctaccgtcg cgccgtcgcc gccaaccttg ccgcgctttc
     3061 agacggcatc gggttgccga aacacgaaaa actgcttgcc gccgtcgaaa aagtcatttc
     3121 agacgacctc ttggacaacg ccttcaaagc cctgcttttg ggcgtgccgt ccgaagccga
     3181 actgtgggac ggcacggaaa acatcgaccc gctgcgctac catcaggcgc gcgaagcctt
     3241 gttggatacg cttgccgtcc gctttctgcc gaaatggcac gaattgaacc gtcaggcggc
     3301 gaagcaggaa aaccaaagtt acgaatacag ccccgaaacc gccagctggc gcacgctgcg
     3361 caacgtctgc cgcgccttcg tcctgcgcgc cgaccccgcg cacatcgaaa ctgttgccga
     3421 aaaatacggc gaaatggcgc aaaacatgac ccacgaatgg ggcatcctgt ccgccgtcaa
     3481 cggcaacgaa agcgatacgc gcaactgcct gctggcgcag tttgccgaca agttttcaga
     3541 cgacgcgctg gtgatggaca aatatttcgc ccttatcggc tcaagccgcc gcagcgacac
     3601 cctgcaacag gttcaaaccg ccttgcagca tccgaaattc agtctcgaaa accccaacaa
     3661 agcccgttcg ctcatcggca gcttcagccg caacgtcccg cattttcacg cacaagacgg
     3721 cagcggctac cgcttcatcg ccgacaaagt catcgaaatc gaccgcttca acccgcaggt
     3781 cgccgcccgc ctggtgcagg cgttcaacct ctgcaacaag ctcgagccgc accgcaaaaa
     3841 cttggtgaaa caagaattgc agtgcattcg ggcgcaggaa ggattgtcga aagacgtggg
     3901 cgaaatcgtc ggcaagattt tgggttgagg cagtcaaaca gaaaaaacaa gggtctgtac
     3961 cagattagca gatatgttac cctcgaaata tgaagataac acactgcaaa ttaaagaaag
     4021 aagtacaaaa agaaccgctc cgttcttttg taccggaagt taccgcccgt tctgccgccg
     4081 atattttggg tatccatccc gattcggcgg cactgtttta ccgtaaaatc cgcacggttg
     4141 ccaaccatcg tttggccttg gctgccgatg aggttttcga gggccccgcc gggccgggcg
     4201 gaagct
//
LOCUS       DAXFWM010000104         4197 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000104 DAXFWM010000000
VERSION     DAXFWM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.104"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..228
                     /locus_tag="ACFI8B_002068"
     CDS             <1..228
                     /locus_tag="ACFI8B_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFC3835186.1"
                     /translation="KKTVKFQFPPEVVRVLAGMGNNLNQIAKALNTAAKVGTLGNVEA
                     LKATTELAALERSLNSLRDFLAKEKNGWQSQ"
     gene            225..902
                     /locus_tag="ACFI8B_002069"
                     /pseudo
     CDS             225..902
                     /locus_tag="ACFI8B_002069"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            916..1557
                     /locus_tag="ACFI8B_002070"
     CDS             916..1557
                     /locus_tag="ACFI8B_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFC3835187.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMLDALAELESNHAAIEQR
                     MMKALTHLGERLAELEQENTSLAQQLASLAAELERQSEIRQRQSEILNQPAKR"
     gene            1604..1858
                     /locus_tag="ACFI8B_002071"
     CDS             1604..1858
                     /locus_tag="ACFI8B_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cryptic plasmid protein A"
                     /protein_id="HFC3835188.1"
                     /translation="MITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYPSNIAK
                     VLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1836..2114)
                     /locus_tag="ACFI8B_002072"
     CDS             complement(1836..2114)
                     /locus_tag="ACFI8B_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFC3835189.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2114..2299)
                     /locus_tag="ACFI8B_002073"
     CDS             complement(2114..2299)
                     /locus_tag="ACFI8B_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFC3835190.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2302..2547)
                     /locus_tag="ACFI8B_002074"
     CDS             complement(2302..2547)
                     /locus_tag="ACFI8B_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835191.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2544..2786)
                     /locus_tag="ACFI8B_002075"
     CDS             complement(2544..2786)
                     /locus_tag="ACFI8B_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFC3835192.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2790..3659)
                     /locus_tag="ACFI8B_002076"
     CDS             complement(2790..3659)
                     /locus_tag="ACFI8B_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC3835193.1"
                     /translation="MSQLYTQPDLFLQERIPHKPYCKDFKEAPMLVRSYAAAIKRRYI
                     QVNPPHLRVFMLFDLDYEGAGLAWEDNNLPMPAWAAINRENGGAHLAYALSAPVLTAE
                     YGGRQKALRYLAALEAAYKAKLRGDVGFVSLITKNPEHPHWLTLRGVPDAIRGYDLEY
                     LADFVDLDKFKPYIGRSNVEAVGLSRNCTVFNLVSRWAHKNVLAFKQQGYTVQGWLKE
                     VHYQCMRVNGDFPVPMWEKEVKCISKSIANWVWYKFDIAASNRRFSELQAHRNSLRKT
                     TINAGRTKIITEL"
BASE COUNT         1139 a         1036 c         1129 g          893 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag acggcttaga agccctagcc ctatcaggca
      781 agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt gaaatatcac
      841 gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc aacatccgac
      901 tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga gcagacatca
      961 cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac gttacggaaa
     1021 aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat aaacgcccgg
     1081 cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct aggtatatcg
     1141 gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt caagaacgag
     1201 ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct gttggaaacc
     1261 aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc aacgcgggta
     1321 tgcgtagcga ccgagaagcg aatgctggac gcgttagcgg aattagagag caatcacgca
     1381 gcaatcgagc agcgaatgat gaaagcctta acgcacttgg gcgaaaggtt ggcagagcta
     1441 gagcaggaaa acacgagttt agcgcagcag ctagcgagct tggcagccga gttagagcgg
     1501 cagagcgaaa tacggcaacg gcagagcgaa atcttgaatc aaccagccaa acgataagcc
     1561 aacgatacaa acgaacccaa agcaggggat ggggaatgag ccgatgatta ccgagaacga
     1621 acgcgacagg cgaacagccg catggctgat agagacctac ggggcagaag ccgtagcgga
     1681 agcagaaacc cgcattgcgg gcgcgagaaa gccctatccg agcaatatcg ccaaagtatt
     1741 gggggctagc ctacccgaag ccctaaaacg cacagaaaac gccgcagcgc gccaaaaact
     1801 ggcagggctg cggcggatgt tggacggtaa ggcagttaag acttcacaag acttgtgaaa
     1861 tcagaccact gctcaatgcg gaacgcccga atatcgcgga cagaagacgg aaaccaaggc
     1921 agagctttta gctcgttgat ggctgaaaac aggttagcca tatcttcgtt ttggcaggtg
     1981 tacaaacttc cctgaatgcg ggtaaagccg aatttccgca gggtgtatcc aatatccgcg
     2041 taggcttggg aaatgccttt cggatggttt tgagcggtat cggcaaccac caaatcaaaa
     2101 gaaatcgcgt acattaggca gcctttgggt cttgaacgat gtgggacagt ttgtattgcg
     2161 tgtgttcgcc gctttccggc agttcaatat ttacgagcca atcgccgtcc ggaagctgtt
     2221 cggcaggcgt gccgacgatg tacatcacgc cgtaatcgcc aaaggtgcgg attgaaccgg
     2281 tggggatagt ggtttgcatg gttagttctc ctgttgggtt tggttttcag gctggtttgt
     2341 catgtacaaa acccgttggg cagctttttc ggctgttgtg cgtttgatgc cgtcaggcag
     2401 aaaaattctc cattttttgg ctagaactat atctgcaaga tcatcaaccg ttgcgctttt
     2461 caaagcagga tttgcgttaa tttccgaata cagaaaatct ttgaatgccg agtacagagc
     2521 atcgcggttt attgcgtatc ttgtcatgct gtttcgcttt ggttgtcagt ttcagacggc
     2581 ataggcttgc cgcgcctgta ccaagtggcg cgggaaatgc cgaagtgttc ccacggttta
     2641 tcgcggctga tagagttttc ggctaggtag tcggcgcggg attgcgacca tgcccgaaaa
     2701 acggttcttg tggaacagcc gaagcgtttt gccagttctt tagccgtaac gtctcgcttg
     2761 gtggttttca gttttggata ggccatattt caaagctccg tgatgatttt tgttctgcct
     2821 gcattgatgg ttgttttccg caaactattc cgatgagcct gcaattccga aaaccgtcga
     2881 ttgctggctg caatatcaaa cttgtaccaa acccagttag cgattgattt ggagatacat
     2941 ttcacttctt tttcccacat cgggacaggg aaatccccat ttacccgcat acactggtaa
     3001 tgcacttctt tcagccagcc ttgcaccgtg tagccctgct gtttgaacgc caacacgttt
     3061 ttgtgcgccc aacggctcac aaggttaaac accgtgcaat ttctgcttaa tccgaccgct
     3121 tccacgttag agcgaccgat atagggctta aacttgtcta aatccacgaa atccgcaaga
     3181 tactccaaat cgtagcccct gattgcgtca ggaacgccgc gcagcgtcag ccaatgcgga
     3241 tgttcgggat ttttcgtaat cagcgataca aagcccacat caccgcgcaa tttcgcctta
     3301 tatgctgctt caagtgcagc aagatagcgc agggcttttt gtctcccacc gtattccgcc
     3361 gtcagcacag gcgcggaaag cgcataggca aggtgtgcgc cgccgttttc cctgttgatt
     3421 gccgcccaag caggcatagg cagattattg tcttcccaag ccaaccccgc cccttcgtaa
     3481 tccaagtcaa agagcataaa cacacgcaga tgcggcggat tgacttggat gtagcggcgt
     3541 ttgatggcgg cagcgtaaga gcgcaccagc ataggcgctt ctttgaaatc tttgcagtat
     3601 ggcttgtgtg ggatacgttc ttgcaagaag aggtcgggtt gggtgtataa ttggctcatg
     3661 ttgtatctcg aaacccccgt gcagattggc gtttggcggg ggttttgctt tgtctaagat
     3721 ttgcagattg tatgcttgtt tttaagatga tacaactatg tcaaaataac cataatcaga
     3781 taacagcccg ataggggttc ttatttcaaa attttccaat ccgcaattta gcgaagccag
     3841 caggcgaagc ggtaaagctt ggagcgcagc agcgcgacct aagccggcca gcagggcggc
     3901 gttttggggg aaacatgaaa ccagttccga cagggcggcg tgcgtgttct tcccggagtt
     3961 cttcatggag tatcggcgaa atgccgtgat gaaatgccgt ttttttgagc agaaagcagt
     4021 caaaaacagg ggtattttgc ccttttgaca ggttcgagtg ccgccgaaaa gcgaacaaag
     4081 caactcatca tccgagtcag cccgaccgag tttgagactt tgacccgaca gaagacccat
     4141 ccgaatttag cccgctacat tcgggagcgg gttttggaag atggcaaagc atccgac
//
LOCUS       DAXFWM010000105         4183 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000105 DAXFWM010000000
VERSION     DAXFWM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.105"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            536..2002
                     /locus_tag="ACFI8B_002077"
     CDS             536..2002
                     /locus_tag="ACFI8B_002077"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3835194.1"
                     /translation="MASLRPTLNQLSSWQEHLYSDVDPFHYIEVTDNSHVIGQTISLD
                     EFHLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKV
                     PETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIY
                     KLSLQQRQDGFFTKVQERDDIAIYAQQAQAANTLFNLRLNDKNSNIFDRTLPRKGLWL
                     RVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFRN
                     PDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYVDSWMQYQRFRHRINTEYATERFTS
                     KGITASIEAGHNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAQVNLLGS
                     RQLQSRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEIDGDRRVINNKTVIETQ
                     LGVAAKIKSHLTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(1985..2290)
                     /locus_tag="ACFI8B_002078"
     CDS             complement(1985..2290)
                     /locus_tag="ACFI8B_002078"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835195.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(2331..3698)
                     /locus_tag="ACFI8B_002079"
     CDS             complement(2331..3698)
                     /locus_tag="ACFI8B_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC3835196.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(3808..4074)
                     /locus_tag="ACFI8B_002080"
     CDS             complement(3808..4074)
                     /locus_tag="ACFI8B_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835197.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEAAAAEAPAAEAPAAEAPAAEAAATEAPAAEAPAAEAAK"
BASE COUNT         1189 a          898 c         1001 g         1095 t
ORIGIN      
        1 aaaatcattt attataagtc tatttttttc tattctttat acctctccgc ttttggctgt
       61 tgattacgtt tacgacaatt ctaaattaac aaatgagcaa attgatcgct taaaaaaaac
      121 ttagagataa aagcagcgaa tactggaaac aagaaactta tcttttaaaa ccgacgaatg
      181 gtagctctca gaactctctc aaagttcccg cactatcctc taaaacacac caattcgaaa
      241 acatcaacaa ctcaaaaaag atttcttttt atgacaagga atacactgaa gattaccttg
      301 ttggcttcgc ccgaggtttc ggggttgaaa aaagaaatgg ggaagaagaa aaaccactcc
      361 ggcaatattt taaggactgt gtaaatactg agaattccaa taatgataat tgtaaaattt
      421 catccttcgg aaactatggc ccaatattaa taaagagcga tattttcgcc ttagcatcac
      481 aaataaaaaa tagccatatc aacagcgaaa ttttatctgt aggtaattat atcgaatggc
      541 ttcgcttcgc ccaacactta atcagctttc tagttggcaa gagcatctct attctgatgt
      601 agatccattc cactatattg aggttacaga caactcgcac gttatcgggc aaacgattag
      661 tttggatgaa ttccacttag aaaattctct ctgggaaccc cgttgggatt ctgatgttgg
      721 ggagttaaaa acaacgaatg cagacatccg ttttaatact aaaagtgaaa gtttactcgt
      781 aaaagaagat tatgctggcg gagctcgttt tcgttttgct tacgatctaa aagataaagt
      841 ccctgaaaca ccagttttaa cttttgaaaa aaatataact ggcacttccg acatcatctt
      901 cgaaaatcca atagacgatt taaaaagcct cgacgggcat caaattatca aagtaaatgg
      961 cacagcagat aaacacgcat tccgtctttc tggcaaacat caaaagggaa tttataagct
     1021 ctctttacaa caacgccaag atggcttttt taccaaagtg caagaacgcg atgatattgc
     1081 gatttatgca caacaggctc aagccgccaa tactttattc aacctacgtt tgaacgacaa
     1141 aaacagcaat atttttgacc gcactttacc acgcaaaggc ttgtggttgc gtgtgattga
     1201 cggacattcc aaccaatggg tgcaaggcaa aacagcacct gtagaaggct atcgaaaagg
     1261 tgtgcaactc ggcggcgagg tgttcacatg gcaaaacgaa agtaatcaac tttctattgg
     1321 cttaatgggc ggacaagcag aacaacgcag cactttccgc aatccggata cagacaatct
     1381 tacaacggga aatgtgaaag gctttggtgc gggcgtttac gccacttggc atcagcttca
     1441 ggacaaacag acaggtgcgt atgttgatag ctggatgcaa tatcaacgtt tccgccaccg
     1501 cattaacact gaatatgcta cagaacgttt tacttcaaaa ggtattactg cctcaattga
     1561 agcgggtcac aatgcgttat tggcggaaca cttcactaaa aagggcaaca gccttcgtgt
     1621 ttacctacaa ccacaggcgc aattgactta tttgggggta aacggaaaat tcagcgatag
     1681 cgaaaatgcc caagtgaatt tgcttggctc ccgccaatta caaagccgag tgggcgttca
     1741 agctaaagcg caatttgctt tcactaatgg cgtcactttc caaccatttg ttgctgtcaa
     1801 ttcaatctac caacaaaaac ctttcggtgt ggaaatagac ggggatcgtc gagtgataaa
     1861 caataagacg gtaattgaaa ctcaattagg tgtagcagcg aaaatcaaat ctcacttaac
     1921 cttgcaagcc tcattcaacc gccaaacaag caaacatcat cacgctaaac aaggtgcgtt
     1981 gaatttacag tggacgtttt aagttaaatt gtaacaaaat aaaatcggca gggaaattca
     2041 ctgccgattt tattatttta aaagtgcggt catttttcgt gtaatttcca ccaattttta
     2101 ccgcactttc aatttcagct tgaacaagtt caatagtctg ctctgcatta attacgactg
     2161 cttttggatt atcttttact aattctaaat agcgtgctcg agtgcgatgg aaaaaatcta
     2221 aatccatttg ctcaatacgg tctaactcgc cacgtccacg agctcgcgct aaaccgacaa
     2281 tcggatctat tgtccttttc tttgttgtgt atgcaaatca gaaatgccgg ttacggaagc
     2341 agtttgccag gattcataat gttatatgga tcaaggtgtt gtttgatgct tttcatcagg
     2401 gcaatttcag agggcgtgcg gacagcgggc agccattgtt ttttgatgat acctatgccg
     2461 tgttcggcgg caatcgtgcc gttgcgggca aggacgttgc gatagactgt gctgttgatg
     2521 tcgttttcgt aacggtaaac ttcattgctg aggatttcgg gcgggaaagt attgtagtgc
     2581 aggctgccgt cgcccagatg tccgaagcag acgatttgta tgcctttgaa attctgttcc
     2641 aaatctttgg cgcaccggcg gacaaagtcg gcaacgcgcc cgataggaac ggcaatatcg
     2701 tgtttgatgc tggtgcccag tttgcgttgg gaggcggaga tgttttcgcg caacgcccac
     2761 atatggatac gttcttgttc gctttgcgcc aatacgctgt cggtaaatcc ttttttataa
     2821 agaaattcga caagccgatc atcaagattg ctgtcgggta atgagtcggt caactcaagt
     2881 aaaatatgcc attctgaatg tgtcggcagg gggagtttgc tgaattcgga agacaactcg
     2941 gcggcaaaac ggccgatcag ttcaaaactg cataagcgtt cggcaaagtg cgcttgggtt
     3001 tcggtcagca ggcggacggc ggattcgatg tcgggaatgc cgacccatgc cgttgccttg
     3061 tccgagggac gggcaaaaag cttgagcgtg gcggcagtga tgatgcccag tgtgccttcg
     3121 ctgccgataa acagatggcg caggtcgtag ccggtggtgt ttttatgcag gggatggaga
     3181 tgggaaacca gttcgccgtt ggggaggacg acttccaaac cgataaccag gtcgcgcatc
     3241 gtgccgtaac gcaatacgtt caaacctccg gcattgcagg cgatgttgcc gccgatttgg
     3301 cacgagcctt cgctggcgag actgagtggg aacagcctgt ttgaggcttc ggctgcctgt
     3361 tggacggttt ggagtacgga acctgcttcg acggttatgc agttgtctga caaattgatg
     3421 ctgcggatgc ggttgagctt ggaaaggttc agcaatacgc cgttttccga tactgccgcg
     3481 ccgcacaaac cggtattgcc gccttgcggc gtaaccggaa tgcggtgttc gtaacaaaaa
     3541 cgcataatgg tttgcacgct ttcaacgctg cgcggctgca aaatgatgtc tggtgcagac
     3601 gtaaagcggt tgcgctgatc ttttaaaagc gtcggagaag gttcggcaat ttcatctgcc
     3661 ggcaggagtc gggaaaattc ggtatgaagg tcaggcatgt ttgtcggaag ggtagggaga
     3721 agattgagga agggaatctg atgccgtctg aaaaatcaaa aaagcaggat actgaacgta
     3781 tcctgctttt ttgcaagcgg aaaatgctta ttttgcagct tcggcagcag gagcttcagc
     3841 ggcaggtgct tcggtagcag cagcttcggc ggcaggagct tcagcagcgg gagcttcggc
     3901 ggcaggtgct tcagcagcag cagcttcggc ggcaggtgct tcggcagcgg gagcttcggc
     3961 agcaggtgcc tcggtagagg aagcctcagc agcaggagct tcagcggctt tttctccgcc
     4021 gcaggcagcc aaagccaaag acaacaaagc agcggcaaac agggattttt tcattttagt
     4081 aacctttaaa atcaatttaa attattgaaa caaataggtc tttttcgacc atactacaaa
     4141 ccgggttgga aattcatcgc tgaatttgcg taattatgca cca
//
LOCUS       DAXFWM010000106         4098 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000106 DAXFWM010000000
VERSION     DAXFWM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.106"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..173
                     /locus_tag="ACFI8B_002081"
     CDS             <1..173
                     /locus_tag="ACFI8B_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFC3835198.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1456
                     /gene="cas8c"
                     /locus_tag="ACFI8B_002082"
                     /pseudo
     CDS             170..1456
                     /gene="cas8c"
                     /locus_tag="ACFI8B_002082"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFI8B_002083"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFI8B_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="ACFI8B_002084"
     CDS             2263..2631
                     /locus_tag="ACFI8B_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835199.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..3288
                     /gene="cas4"
                     /locus_tag="ACFI8B_002085"
     CDS             2638..3288
                     /gene="cas4"
                     /locus_tag="ACFI8B_002085"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFC3835200.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNAQAWAE
                     NCLTAQGKALHERVDSGEPETHKGVRFERTVHVSAEKPGISGILDLVEADTKTGRLKP
                     VEYKRGKPKPDPGDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRAQ
                     TLATIAAVCKLLNSGQTPPPDYGKRCKACSLAEICQPELLGKRDRSVGYVKGLFGE"
     gene            3404..3763
                     /locus_tag="ACFI8B_002086"
     CDS             3404..3763
                     /locus_tag="ACFI8B_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835201.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSEN
                     DLEEIREERERLGLEDD"
     gene            3905..>4098
                     /locus_tag="ACFI8B_002087"
     CDS             3905..>4098
                     /locus_tag="ACFI8B_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835202.1"
                     /translation="MTQKNDFKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYT
                     PEPIWRSPITMAWLTWASFL"
BASE COUNT         1203 a          915 c         1007 g          973 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcggcga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aagcactcgg cattgagtct gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 cattaccgcg tggatgtgta aaaacttctt cgatatccga accttcggcg ctgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacacgcatg gcggtaacca acgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgtgg tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctcagccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacaccg taaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgcgcaggcg
     2761 tgggcggaga actgtttgac cgcgcagggc aaggcactgc atgagcgggt ggattcgggc
     2821 gagcccgaaa cgcacaaggg cgtgcgtttt gagcggacgg tacacgtttc ggcggaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata caaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggatgaaat ccagctttgc
     3001 gcccaaggtt tgtgtttgga agaaatgacg gggcaaaccg tttcagaggg cgcgctgtgg
     3061 tatatgcaaa cccgccaccg cgtccccgtt gtgttttcag acggtctgcg cgcccaaacc
     3121 ctcgccacca tcgccgctgt gtgcaaactc ctaaacagcg gccaaacccc gccgcccgac
     3181 tacggcaaac gctgcaaagc ctgctcgctg gcggagattt gccagccgga gttgctggga
     3241 aaacgggata ggagtgtggg atatgtgaag gggttgtttg gggagtaata ttttatttga
     3301 aaaaaactat tacgttaatt aacttgaatc ttccaaatta atcgcaattt ctgaaaacga
     3361 gcaatcatgc tcaattgtaa ttacacattt taaggagtcg taaatgacaa gaaatataga
     3421 acatcaattt agcaatctat ctgatgttgg tgaaaaacta gagttagaaa atcctactgt
     3481 agagaatgtc gtagatattt tagtggatat tggacatgat gaccgagttt atacttttca
     3541 tgatgacttt ctcgggttaa aaagcggttt gccacaagac ttattgtcga aacatatcga
     3601 cgagcttgaa gaaggtgatt ttgcagatag atattcagat gaaattgata aaatactgga
     3661 taatgcaaat ataatattct atcaccttga acgagagctt agtgaaaatg atttagagga
     3721 aatacgagaa gaaagagaaa gactgggttt agaagacgat tagtatactt gttgtcatag
     3781 aatagattca atacatacag gttgggttat gctgttttac ccaaccagct aacctcaggg
     3841 catatttaat attatttaat tctgaataat attctattga agtttcaaga tatctaagga
     3901 aaacatgaca caaaaaaatg attttaaaaa tgctaatttt tatgggaata ctcaaatagt
     3961 taatggtgat gttcataatg tacatactca aaatacacca aattcaataa atgaaaacga
     4021 aatagccaca tacaccccgg aaccaatttg gagaagcccc attacgatgg cttggctgac
     4081 atgggctagt tttctgat
//
LOCUS       DAXFWM010000107         3741 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000107 DAXFWM010000000
VERSION     DAXFWM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.107"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            69..1193
                     /locus_tag="ACFI8B_002088"
     CDS             69..1193
                     /locus_tag="ACFI8B_002088"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3835203.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVTENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            1190..2227
                     /locus_tag="ACFI8B_002089"
     CDS             1190..2227
                     /locus_tag="ACFI8B_002089"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC3835204.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            2228..>3741
                     /locus_tag="ACFI8B_002090"
     CDS             2228..>3741
                     /locus_tag="ACFI8B_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HFC3835205.1"
                     /translation="MLRTYLNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     SDKPAKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTV
                     YSSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPS"
BASE COUNT         1211 a          669 c          757 g         1104 t
ORIGIN      
        1 agagcatagt tcacctttaa aatccataca aggattttta tcaaatttat gcataggagt
       61 gaaatcctat gctatctaaa caaatctcaa atcttaattc ttctagtaac aaaccaaaaa
      121 tcctatctct attttcagga tgtggcggtt tggatttggg ctttcatcaa gctggttgtg
      181 aaactgtttg ggcgaacgat ttctcccatt gggcttgcga aagtttccgt aaaaatatcg
      241 gcgatgtcat cgtagaaggt gatattgaac aaattaatcc gaatgatcca actattcccg
      301 attgcgacat cattttaggc ggattccctt gtcaagattt ttccatgatt tggaaacagc
      361 cgggcttaga gggtgagcgc ggcaatcttt ataaaagctt tttacgtttt gtaaatgcaa
      421 aaaaaccgaa agtttttgtt actgagaatg tgaaaggttt attgactgcc aacaagaaaa
      481 aagccatcca gcaaattatt accgactttg aaaattgcgg ttattacgtt caggcgaagc
      541 tgtataactt tgcagaattt ggcgtacctc aatttcgtga acgtgtgctg attgtcggag
      601 tacgtttgga tacaggattt gattttcgcc atccggaacc gacgcacaat gaaactggcg
      661 aaaacggctt aaaaccatat gtaacagcag gtcaggccat atccaatatt ccacaaaatg
      721 ccagtaataa tgaattactg aaaatcagcg ataaaacacg ccgtatgttg gaattaattc
      781 ctgaaggtgg aaattttacc gatattccta aagatcatcc tttatatgtg aaaggtatga
      841 ttagccacgt ttatcgtcgt atgcatcgga acgagccatc aaaaacaatt attgcagcag
      901 gtggcggtgg tacttggggc tatcacttcc ctgaaccgcg tgcttttact aatagagaac
      961 gagcaaggct tcaaagtttt cctgatgatt ttgagtttgt cggatcaaca actgaagtac
     1021 gtcgccagat tggtaatgct gttcctcctc agggcgtggt tgaactggca aaaagcattt
     1081 taccgatttt ttcagacaac tatgagaaag tagatttgca tgagaaatta gtcgaagaaa
     1141 aagaaatttt attccatgac cgactaagca aaattcgagg gggaaaacaa tgaatacagt
     1201 tttttccaat atcgccaatg ccaaaatcac tgaaaaatct cttaatgcgg tttggatgga
     1261 tttatttaaa tcagcggatg aagtgttgat ggcgacaggt tatgtatcta atgacgcagt
     1321 ggttgagtta cataaaattt tggagctaaa cgatcatatt caaaaaatag atttattggt
     1381 cggaatgcat tatttggaag gttttagcca tttacaatat gacagcttat gtaaactaaa
     1441 tgattttttg cgacatgaga aaagaggagc cgtctatgtt tccccctttg tgaaatttca
     1501 cggcaaaatg tattccttta aaaattatca aaaaatcaat ggattaattg gttctgctaa
     1561 cttaacgtgt ttttgggata gcacggaacg tacttatgaa actatgttgc atttgaacgg
     1621 taagcctgca cagattttgc aggcagatat tcaaagtact attcataagc taggcaaaaa
     1681 tatccaagaa gttgaaagac caagtaaatt tattgaacat aatagccatt tagaaaattg
     1741 tttgggtgtt cagaaaattg caccagaaca gattcggcaa ttatttgccc aaacgtcaga
     1801 atatcatttc tcaattccag ctaaaaccga agaaaaaagt aatttgaatg tattttttgg
     1861 agaaggtcga cgagataaac gagggtttgt caaaccacga ccttggtatg aagtagagtt
     1921 gattgtatcc aaagacatca cttcacaaga agggtatccg gttctgaagt cttttacagt
     1981 tattaccgat gatggttggc aatttcaatg caagacatcg ggggattact ccaaaaactt
     2041 ccgctcagaa aatgatttaa agacacttgg taaatggatt aaaggtcgtt tggaaagtca
     2101 tggctgcctg caaaataacg agaagattac gcatgaaacc ctacgcgaat acggtaacga
     2161 tcattttgag ttacgttcaa ccgataatcc tgatgtttgg ctgctctcat ttaaggggaa
     2221 aaactaaatg ttgcgaacct acttgaacca attgacccct cccgaattag ctgattctgt
     2281 taaaaatacc gttgatggtt ttatggaaaa attgtcacaa accgagccga aaatcgccca
     2341 aaatgtttta ttgctgggca atgttcaaag tggcaaaacg gcacaggttt tgggtgtatt
     2401 aagtgcgctg gctgatgatg gcgaccacaa agtttttttg tatcttacta ctgacagcgt
     2461 agatttacag gatcaaacag tcaaacgagc caaagccaat ctgaaaaact ttatcgtatt
     2521 gtctgaagct gacgaccgaa gttttatgga agtaatgaag gcagagaacc ccatcttggt
     2581 cgtgattaaa aagaatgccc gtgtattgaa acgttggcgt aatttgtttg ccagtcaaag
     2641 tagcctgaaa ggctatcctt tggttattgt ggacgatgaa gccgatgctg ccagcctaaa
     2701 tactaattct gataagccgg ctaaagatgc cagtactatc aataaactgc taaacgacat
     2761 caaaaattcc tgttgtcaaa gcctgtttat ccagttgact gccacgcctc aatcgctttt
     2821 attacaacat gaagaatctg attggcaacc tgaatttatc cacttctttg aagcaggcga
     2881 aaaatacatc ggcggcaact ttgtcttttc tgatccacca agctatatag ttcgttttat
     2941 tgatagcgaa ttggatgata tgaaagacga gagtggtgaa attgccgaag gagcaaaaca
     3001 ggcattgctt agttttctaa taacctgtgc agaattcgcg ctatgtgata aggctaactg
     3061 taattttgca ctacatccaa gctataaaat tcaagatcat caagcctttt caaagaagat
     3121 ccaagccttt ttaaatgact tagtgcaagc agtcaataat ggggaagacc ttgcaggtag
     3181 ctttaaagaa agttatttgg atttgcaaaa gaccaagccc gatattcatc actttgatga
     3241 aatatacgaa aaattgacag cacttttaga gaataaacaa atctctactc ttgtcgttaa
     3301 ctcgcagaca gaaacagatt ttgacttaga aaaaggtttc aacatcatta ttggtggtaa
     3361 tgtgattggt cgtggcttga ctattccgaa actacaaacg gtttattcta gtcgcactgc
     3421 caaaaaaccg aatgcagata ctttctggca acattcgcgt atttttggat atgaccgcga
     3481 taaatcatta ttacggcttt atattccgtt tgatgtctat tacttctttg tacaactcaa
     3541 tcaagcaaat aatctgatta ttgggcaggc aaaaaattca ggcggcaata ttcaggttat
     3601 ttatccgaaa aatatcaatc caactcgaaa aaatgtattg aaattcgata gtatcaatca
     3661 aattgtgggt ggtgtgaatt attttccact ccacccaaac gaagataatt tatcggagat
     3721 taacaaaatt ttgccttcta t
//
LOCUS       DAXFWM010000108         3696 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000108 DAXFWM010000000
VERSION     DAXFWM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.108"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..454
                     /locus_tag="ACFI8B_002091"
     CDS             <1..454
                     /locus_tag="ACFI8B_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3835206.1"
                     /translation="IASLRRGAIYKVWQFVEDALRAVVPADSFEPTAQKLKLFKAGAA
                     TILFYEDQNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGAGANLQHYNP
                     LPDVAIAKAWNIPENWLLRAQMVIGGIEGAAGEKVFEPVAERLKVFGA"
     gene            complement(509..964)
                     /locus_tag="ACFI8B_002092"
     CDS             complement(509..964)
                     /locus_tag="ACFI8B_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFC3835207.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1220..2590)
                     /gene="ffh"
                     /locus_tag="ACFI8B_002093"
     CDS             complement(1220..2590)
                     /gene="ffh"
                     /locus_tag="ACFI8B_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC3835208.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            2810..3616
                     /locus_tag="ACFI8B_002094"
     CDS             2810..3616
                     /locus_tag="ACFI8B_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC3835209.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          812 a          917 c          998 g          969 t
ORIGIN      
        1 tattgcgtcc ctaagaaggg gcgcaatata taaggtgtgg caatttgtcg aagacgcgct
       61 gcgtgccgtc gtgcctgccg acagttttga accgaccgcg caaaaattga agctgtttaa
      121 ggcgggcgcg gcaaccattt tgttttatga agatcaaaat gtcgtcaaag gtttgcagga
      181 gcagttccct gcttatgccg ccaactttcc cgtttgggcg gaccaggcga acgctatggt
      241 acagtatgcc gtctggacga cacttgccgc ggtcggtgca ggtgcaaatc tgcaacatta
      301 caaccccttg cccgatgtgg cgattgctaa agcgtggaat attcccgaaa actggctgtt
      361 gcgcgcgcaa atggttatcg gcggtattga aggggcggca ggtgaaaagg tctttgaacc
      421 cgttgcggaa cgtttgaaag tgttcggcgc ataatttcgc ggtcaaaaaa atgccgtctg
      481 aaccccgttc agacggcatt tttcagtatc aggcggcgag tttttcgcat tctttgatac
      541 agtcgaggca ggactcgtaa caggctttgc attccgcatg gtgggcggag tgttctttac
      601 aggctttggc acactgtttg cacgcttcga ggcacacttt tgccgcgtcg cgtgtcagag
      661 gggaattttg tgcggcaagg tcgtgcaccg cgccgcataa ggcaagcatc tggcgcacgg
      721 caaccgcaca gtcggacata gacgtgtcgc cctgagtgag cagggacagg cagtgggtca
      781 ggcaaacctg tccggcatcc agacagtgtg cggcagtttg gcggacggcg gtgtaggcgg
      841 atgcggcggc aggctgcata tcgtgatgat ggtggtagtc ggcgtgtccg tgcgcacgcg
      901 cgaaagaggc ggcggaagcc aaagagacgg cagcggcgct gcccaaaaat tgacgacggt
      961 tcatgatttt ttcctttatg tttcaacatg cgggtccggt ataaggtaca cacttaccat
     1021 actgccctgg ccggatcggt acgattttct gaataattgc ttgattttgc aggtgtgaaa
     1081 ttgtcgcacg agcaggcatg ttttagctat tcggaaattc gaggaagggt gcatcaacct
     1141 gttttgaaat gatgcgcaga catggtaaca gacaaaaagc cgtctgaaaa tcgttttaga
     1201 cggcttttgt tttcaaacat taaagaccgg gaaacattcc cttcattccc ttcatgcctt
     1261 tggccaagcg catcagtttg cccaaaccgt tgccgctgaa catcttcatc atctgctgca
     1321 tttgttcaaa ctgtttgagc aatttgttca cttcctgcac ggtcgtgccc gcgcccatcg
     1381 caatacggcg tttgcggccg gctttgagca gggcagggtt ggcgcgttct ttaggggtca
     1441 tcgagttgat gatggcttct actttgccca tcgctttttc agccgtacct tcggggattt
     1501 gtttcgatat ttgacccagt tcgcccggca ttttcgacat caggttttcc aaaccgccca
     1561 tattgcgcat ttgctggatt tgttctttaa aatcattgag gtcaaagcct ttgccttttt
     1621 gcagcttttt cgccattttg gcggcggctt cttcatcaat acctttttga acgtcttcaa
     1681 tcagggtcaa tacgtcgccc atgccgagga tacggctggc aagacggtcg gggtggaaag
     1741 gttcgaggcc gttgattttc tcgccgacgc cgataaattt aatcggtttg ccggttacat
     1801 ggcgtacgga caatgccgca ccgccgcgcg agtcgccgtc catcttggtc aatacgacgc
     1861 cgttcagcgg cagggcttca ttaaatgcct gagcggtatt taccgcatcc tgacccagca
     1921 tggcatcgat aacgaacaaa gtttcaaccg ggttgaccac cgcatgaagc gctttgattt
     1981 cgttcatcat ctcttcatcg attgccaaac ggccggcggt atcgaccatc aatacatcgt
     2041 aaaaatgttt tttggcgtaa tcaacggcgg cagttgcaat ctcaaccggt ttttggttgg
     2101 tatcggatgg gaaaaaatct acgccgacct gttcggccaa caggcgcagc tgttcaatcg
     2161 cggcagggcg gtaaacgtcg gcggatacca ccaaaacttt tttcttctga tcgtttttca
     2221 gcaggcgggc gagtttgccg acggttgttg tcttaccggc accctgcaaa ccggccatca
     2281 aaatgatggc aggcggtgaa accgataaat ccagtgtttt gttttccttg cccatcagtt
     2341 cgactaaggc ttggttgacc acgccgataa aggcttggtc aggcgtcagg ctgcccgcca
     2401 cttcctgacc gagggccttt tctttgacgt tgttgacgaa ctctttgacg acaggcaggg
     2461 cgacatccgc ctcaagcagg gcgagtcgga cttcgcgcaa ggcttcttta atattgtctt
     2521 cggtcagttt ggcctgcccc cggatgtttt tgaagacatt gctgaagcgg ccggttaaat
     2581 tgtctaacat actggtcctt ggtctgaata agaatagctt gccccatcag gggcattctt
     2641 tgttaaaata aaatcaaaat aatttgatgc ggcttgtgtg ccggacagca tatcggcaaa
     2701 tccgtcaagg cttgcccgaa acggggattt tacaattcca acgttaaaag ttccaatatt
     2761 tcataagcgg ccgcatacgg cgcaacagta tagatagaga aagtccacca tgccgacagt
     2821 tttcatcttt ttgacggcgg tttacgcagg attgggtgca tttgcatggc actgccaaca
     2881 gcaggggcgc ggccgggatt acccgtggaa gacggaattg ccggttttgg gcgcggcatt
     2941 gaccgtccac ggcgcggcac tgctgatgcc ggtcattcaa gacaaaatca tcattatggg
     3001 cttcgggtat tccggcagcc tgattgtttg gatgatgctg tttatttatt ttgccggcag
     3061 ctttttttat ccgctgcgcg gagtgcagtt gctgctgtat ccttgcgccg cactgatgct
     3121 gctgtcaggt ttggttttcc cgggaaaatt ctcgggatat gaaattaccg actttccctt
     3181 tatgctgcat atcggcacat cactgcttgc atacgggctg ttcggcatcg caacattatt
     3241 gtccgttttg agcttgctgc tgcaccggag cctgcaccgc agaaacttct ccaagctcgc
     3301 aggattcctg ccgtcgctgc tcagtttgga aaaactcatg ttccaggcca tgtgggcagg
     3361 tttcatcctg ctgacctatt ccgtcgtcag cggcacattc tttgccgaag ccgtattcgg
     3421 caaacccatg acatttaccc ataaaaccgt attcggcata ttgtcatggc tgatttacgg
     3481 aggactgctg ctcaaacaca gcatgaccgc atggcgcggc aaaaaagccg ccgtgtggac
     3541 catcatcgga tttgtcagcc ttatgattgc ctatatgggc agcaagttcg tattggaaat
     3601 cattctgaaa agataagaag agccaacaga tgccgtctga agccccgagt ttcaggcggc
     3661 atattcacaa aggcgcatca gccggaggag aagagg
//
LOCUS       DAXFWM010000109         3629 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000109 DAXFWM010000000
VERSION     DAXFWM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.109"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            3..2102
                     /locus_tag="ACFI8B_002095"
     CDS             3..2102
                     /locus_tag="ACFI8B_002095"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC3835210.1"
                     /translation="MLFHTASYLKPEEQAELEKAVAYAFRAHDGQTRKSGDPYITHPI
                     AVATQLALWHMDTQGLCAGVMHDVLEDTGVTKGEMAAVFGNTVAEMVDGLSKLEKLKF
                     EDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTLGSMRPDKRRRIARETLEIY
                     AQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKSRKNRQDVVGKVLRAFGQRL
                     VGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGFRVIVNSIPACYAALGTLHT
                     LYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQIRTREMDAVAEGGIVGHWS
                     YKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHVKVDLFPNEIYILTPKGKIL
                     TLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKLKTGDSVEIITSEHAKPNPA
                     WLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALSSLLPKDVLLSDGIKEKYLA
                     DLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFGSEVRLSSIKVDGQESGHIH
                     FAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPEQQLDADWENMNGQNYRVGL
                     QVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTEGFVEFKFLLKVKNLDQLNQ
                     IIQNLHSIPYIRKVIRS"
     gene            complement(2169..3353)
                     /gene="coaBC"
                     /locus_tag="ACFI8B_002096"
     CDS             complement(2169..3353)
                     /gene="coaBC"
                     /locus_tag="ACFI8B_002096"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC3835211.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
     gene            3437..>3629
                     /locus_tag="ACFI8B_002097"
     CDS             3437..>3629
                     /locus_tag="ACFI8B_002097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835212.1"
                     /translation="MPSEAPSFRRHIHKGASAGGEEEGLLEAAQRLAEIKNLIRQRHD
                     EFPQKNPAESIDCFPLRAYS"
BASE COUNT          949 a          905 c          895 g          880 t
ORIGIN      
        1 ccctgctttt ccataccgcc tcctacctca agcccgagga acaggcggag cttgaaaaag
       61 ctgtcgccta tgcgtttcgc gcccacgacg ggcaaacccg caaaagcggg gatccctaca
      121 tcacgcatcc gattgccgtt gcgacgcagc tcgccctttg gcatatggac acacagggtc
      181 tttgtgcagg cgtgatgcac gacgtattgg aagatacggg cgtgacaaaa ggggaaatgg
      241 cggcggtgtt cggcaatact gttgccgaga tggtggacgg gctgtccaaa ctcgaaaaac
      301 tcaaatttga agatcatgcg gaacatcagg cggagagttt ccgcaaactg attttggcga
      361 tgaccaaaga tgtgcgcgtc atcgttgtca aacttgccga ccgcctgcac aatatgcgga
      421 cgctcggttc gatgcgcccg gacaaacgcc gccggattgc aagggaaacc cttgaaatct
      481 acgcacagat tgccaaccgc ataggtctga ataatgcgta tcgggagctt caggatttat
      541 cgttccaaaa cctgcatccc aaccgctacg agactttaaa aaaagcgatg gacaagagcc
      601 ggaagaacag gcaggacgtt gtcggcaaag ttttgcgcgc gttcggccag cggctggtag
      661 gcgcgaatat agaggccaaa atcaaaggca gggaaaaaaa cctgtacggc atccatcaga
      721 aaatgatggc gaaaaagctg cgctttgccg aggttatgga tatttacggt ttccgcgtca
      781 ttgtcaacag cattcccgcc tgttatgccg cactcggcac attgcacacc ctctatcagc
      841 ccaagcccgg acggttcaaa gactatatcg ccattccgaa aagcaacggt tatcaaagtc
      901 tgcatacgac tttggtcggc ccctacggct tgccgattga agttcagata cgcacgcggg
      961 aaatggatgc cgtggcggaa ggcggtattg tcggccattg gagctataaa tcagattcca
     1021 aaacggtcga tcaggcagtg ctgcacacca atcaatggtt gaaaaacatt ttagacttgc
     1081 aggcaagcag cgccaatgcc attgagtttc tcgaacacgt caaagtcgat ttgtttccga
     1141 acgaaatcta catccttact ccgaaaggaa aaatcctaac tttgcccaaa ggggcaacgc
     1201 ctgtcgattt tgcttatgcg gtgcataccg acattgggca taaaacagtt gccgcgcgtg
     1261 tcaataatgt gatgatgccg ctgcgtacga agctcaaaac cggtgattct gttgaaatta
     1321 tcacatccga acacgccaaa cccaatcccg cgtggttgaa tttcgccgtg tccggcaggg
     1381 cgcgcagtgc catacgccaa tatattaaaa atcttaaccg gcacgatgcg gtcgttttgg
     1441 gagagagcct cttacaaaaa gccttgtcca gtttgctgcc caaagatgtc ctgctttcag
     1501 acggcatcaa agaaaaatac cttgccgatc tcaacgacaa acagacttca tttgaggaag
     1561 tgctgtacaa cgtcggtatg gggcataccc tgcctgttta tgtcgccatg catatcgccg
     1621 aattggcagg ggagcatttc ggcagcgagg tcaggctcag ttccattaaa gttgatggtc
     1681 aggaaagcgg gcatattcat tttgcagagt gctgccaccc tgttcccggc gattccatcc
     1741 gtttgctgtt ggttaaggga aaaggcatga ttatccatag ggatacctgc ccgacgttgt
     1801 tgaagtccga tcccgaacag cagctggatg cagactggga aaatatgaac gggcagaact
     1861 accgtgtcgg gcttcaagtc caatcggaag acagccacgg cctgttggca ttaatggcgc
     1921 aagcgatttc cgattccggt gcggacatcg agtcggtcga aacaccgtct aaatcccagt
     1981 cgggaacgga aggttttgtc gaattcaaat tcttattgaa agtcaagaat ttggatcagt
     2041 tgaatcagat tattcaaaat ctgcacagca tcccatatat ccgcaaagtc atcagaagtt
     2101 gaagcaggta aaacgggttg cctgttaatc aaaaggcaac ccgttttatg gtttatccgt
     2161 tcaattgctt atttgttcaa atatacggca agcctttcaa caatccgcat tgccgcttca
     2221 tctttacttg tttcggggaa agacaactcc gcatcatcat ctatgataac aatttggttg
     2281 gttgtcttgc ccatcgcgat tgaaacatca ttggcaacta tcaccggtat ttttttctta
     2341 atacgttttt cccgcgcata tgccattaca ttctccgttt cagcggcaaa accgatgcag
     2401 aacggcgggt tcggcaatga ggcaatagaa gccaaagtat cggggttctc atccaattcg
     2461 atggataacg gtttggcatt tttatctttt ttgaattttt gcgtactcct gtttttaacc
     2521 ttatagtctg agacggcggc aacagaaata aaagcatctt gtttttcaat caaacgatgt
     2581 actgcgcgat gcatatcttc ggcactgacg gcttgaaccg tatcggatat gccgaaaggc
     2641 agcgtggttt gaagctgtcc gtgaatcagg ctgatttccg caccggcggc acggcacgcc
     2701 cgcgccaaag ctacgcccat ttttccgctg gaggtatttg tgatgcccct gacagggtca
     2761 atggcttcaa atgtcgcacc tgcggtaatc aagattttct tgtcccttaa aattttcggt
     2821 gtccacaagt ctggaagcaa atccagcaat tcggcaggtt ccggcatcct tcccatacca
     2881 ttttctctgc aagcctgttc gcccaagccc ggcatataga cagtaatgcc gtctgaaacc
     2941 agttgtgcga tattccgttg gttggcagga ttcagccaca tttccacatt catcgcggga
     3001 gcgatggcaa gagggcattt ccgtgcggct gccagattgg tcagtaggtt atctgccacg
     3061 ccgttacaga tttttgccac ggtattcata cttgccggcg caatcagaaa aacatccgca
     3121 ttccgggtca ggttgatatg ttccataccg tttgaaccgt tgccgccgtg cgtgtcggtc
     3181 agaacgggat tgccgctcaa agcctgaaaa gtcagcggag aaacaaattc agttgccgag
     3241 cggctcataa ccaccgtaac cgaatgcccc tgttttttca gcagtcgcac caactcgcaa
     3301 gacttatacg ccgcaatact gcccgttaca cctaaaagaa tatgtttgcc cattttgaca
     3361 atttctaatt acgaagaaaa aacagcagcc attctataac aaagtacgga tacattagtt
     3421 accgtttgga tacgaaatgc cgtctgaagc cccgagtttc aggcggcata ttcacaaagg
     3481 cgcatcagcc ggaggagaag aggaggggtt gttggaggcg gcgcagcgtt tggcggagat
     3541 aaaaaacctt atccgacagc gacatgacga atttccccaa aaaaatcccg ctgaaagtat
     3601 tgactgtttt cccctgcggg cgtatagtt
//
LOCUS       DAXFWM010000110         3618 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000110 DAXFWM010000000
VERSION     DAXFWM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.110"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..437)
                     /locus_tag="ACFI8B_002098"
     CDS             complement(<1..437)
                     /locus_tag="ACFI8B_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3835213.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLDQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTGKQDAK
                     LIAQYCRSAKESELVKRQKPTDEQYRLSRMTAAYAQIKSECAA"
     gene            517..746
                     /locus_tag="ACFI8B_002099"
                     /pseudo
     CDS             517..746
                     /locus_tag="ACFI8B_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1104..1553
                     /locus_tag="ACFI8B_002100"
     CDS             1104..1553
                     /locus_tag="ACFI8B_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3835214.1"
                     /translation="MNTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYLNRQKTR
                     YLRDRRGRPAHPLSSMFKAVLPGQWHSLSDPELEHSLITRIGFNLFCRFDEPGIPGCS
                     TLCRYRKFRYARAAYFGLLKVGAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(1769..2797)
                     /gene="trpD"
                     /locus_tag="ACFI8B_002101"
     CDS             complement(1769..2797)
                     /gene="trpD"
                     /locus_tag="ACFI8B_002101"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC3835215.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRNIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(2861..3451)
                     /locus_tag="ACFI8B_002102"
     CDS             complement(2861..3451)
                     /locus_tag="ACFI8B_002102"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC3835216.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
BASE COUNT          856 a          973 c          834 g          955 t
ORIGIN      
        1 attgccgcgc attcgctttt gatttgcgcg tatgctgcgg tcatccgtga aagcctgtat
       61 tgctcgtccg taggcttctg cctctttaca agctcgcttt ctttcgccga ccggcaatac
      121 tgcgctatca gctttgcatc ctgtttgcct gttttggttc gcttgaacct gctttctgca
      181 tacttgctta ttttcagcgg gttcactacg taaacgctgt aatactgcgc gaagtagtcg
      241 gcaacttctt cataatagtt tcccgttgcc tccatgcaga tatgcagatt ctgacatccc
      301 aagcttttca accggtccga aaactgatct aaaccttttg aatcgttgtc aaactttgcc
      361 gaatattcgg cattgccgac aatggccgtt gcgtcaaatg tcagtttcga tatgtccaat
      421 cctacggtgt tacgcatggg attaccctta tatattgcgt ccctaagaag gggcgattaa
      481 caaaaattaa tgccctttct tagggacgca atatataagg tttataccgt cgttgtccct
      541 aatgcgcaat cagcgacatt gttgccaatt atccgaaaga aagttaagcc tgatggcatt
      601 gtatacacgg atacctttcg tagttatgat gtgcttgatg ttagtgaatt tagccattta
      661 cgtaagttta acggcattcc caaagagcat ttggggctat atttaaagaa atgccaatgg
      721 catttgaaat agaatctcaa atttagattt taagacaatt agttaatggg aatttggtct
      781 ggttatctag tacaacccca aaagttttta tataaaaaca gtttggtaag ttctcttcgt
      841 gaagtgctta tgttttgcca caaaagttcc ataatcatgg cggcatcacc caagctccat
      901 ccttccaaga aaatggcatc tttgttgtgg ttgaaaatcg actccatacg attttggaca
      961 agcataggga tagatgttct gaaaaacggc agcgcgggca ttctccaacc accttcccaa
     1021 atcttataat acaaacccga agacctttgc aaaattcccc taaaatcccc taaattccca
     1081 ccaaggcatt taggggattt cccatgaaca ccttcttccg gcaaaccgca caagccatga
     1141 ctgccaaaca catcggccgc ttcccgctat cggagttgga ccaggtgatt gattggcagc
     1201 cgatcgaaca atacctgaac cgtcaaaaaa cccgttacct ccgagaccgc cgcggccgtc
     1261 ccgcccatcc cctgtcgtcc atgttcaaag ccgtcctgcc cggacaatgg cacagcctct
     1321 ccgatcccga actcgaacac agcctcatca cccgcatcgg tttcaacctg ttttgccgtt
     1381 ttgacgaacc gggcatcccc ggttgcagca ccttatgccg ctaccgtaaa ttccgctatg
     1441 cgcgggcagc ctatttcggg ctgctcaaag tgggtgcgca aagccacctg aaggcgatgt
     1501 gtttgaacct gttgaaagcc gccaacaggc taagtgcgcc cgctgccgcc taaaaggcgg
     1561 cccggatgcc tgattatcgg gtatccgggg gggggattaa ggggatattc gggtaaaatt
     1621 aggaggtatt tggggcgaaa acagccgaaa acctgtgttt gggtttcgga tgtcgaagga
     1681 agggcttttt tgcaaaggcc tcatgccgtc tgaaacgcca attccgccgt tcagacggca
     1741 tttgttgctt tatatcaagt ttgccggctt aggcgaattg ccgtgtaaaa ccgacaaact
     1801 cctctttttt cgccttcgcc ctacctgaat cgatgccttc ccgtgcggca gatatgccgt
     1861 ctgaaagcga agcagcgata tttccggcat ataaggcggc ggcggtatta agcaatacga
     1921 tatcgcgcgc cgccccttct ttcccgtcca gtacctcgtt cattttcaac aaagattcct
     1981 gagtattggc aactttgatt tcatccaaat tgcggcgggt ttcgataccg aaatcttctg
     2041 ggcggatgtc gtattcactg atttttccgt ctttgagttc ggcaacgcgc gttttgcccg
     2101 tcagtgtaat ttcatccaaa ccgccctccc cgcaaacgac caaaacgtgt tttgagccga
     2161 gttgttgcaa gacccgcgac aaaatgccgc acaaatcggt gtggaacacg cccaaaagct
     2221 ggttcggcgc gcccgcagga ttggttaacg gacccaatat gttgaaaatg tttcggaaac
     2281 cgagcgaacg gcgcaccggg gcgacatggc gcatggcact gtggtgattg ggtgcgaaca
     2341 taaaccctat accggtctgc ctgatacttt gggcaatctg ttcgggagtc aggttgaggt
     2401 ttgcgcccat ttgctccatc acgtcagccg caccgctgga ggaagagacc gaccggcctc
     2461 cgtgtttggc aacctttgcg cctgccgctg cagcaacaaa catcgaagtc gtcgaaatat
     2521 tgaaggtttt cgcgccatcc ccgcccgtac cgacgatatc gaccagcccg tccgcatcct
     2581 ccagcggcac ttttgacgca aactcgcaca tcacggcggc ggcggcggtg atttcggaaa
     2641 cggtttcaac cttgatacgc aagccggtca aaatcgccgc aatttgttcg ggcggcactt
     2701 ttccgctcat catttggcgc atcaagtcgg tcatttcatc gtaaaacaac tcgttattgc
     2761 tgattaatcg ttcgatagcc tgttgcggtg taatcatttt ttgtcctccg ttcaatattc
     2821 ggacgaaaat gccgcccgaa gggcttcaga cggcatcacg tcagattttt tgcggtttga
     2881 agttttgaaa ttcaactaaa aaattgttca acatatcatg tccgcgttcg gtcaagaggg
     2941 cttcggggtg gaactgcacg ccctcgacgg catattcctt atggcgcacg cccataattt
     3001 cgccgtcttc cgtccacgcc gtgatttcca agcaatccgg cagcgtgccg cgttcgataa
     3061 cgaggctgtg ataacgcgta caggtaaccg gattgggcaa acccttaaac atacccttgc
     3121 ccgaatggga cacgggcgac accttaccgt gcatcaaggt ttttgcccgt accacatctc
     3181 caccgaacgc ttcgcctatc gtctgatgcc cgaggcacac gcccataatc ggcagccggc
     3241 cggcaaaatg gcgcatggct tctactgaaa tacccgcctc cttaggggaa cacggtccgg
     3301 gaccgataac gagatattgc ggattcaatg cctcgatttc ctccaacgta atatcatcgt
     3361 tgcggcgcac ggcgacttcc tgccccaatt ctgcgaaata ctggacgatg ttgtaagtaa
     3421 aactgtcgta attgtcgata aacaaaagca ttttgtgatt aacctattga tttataattt
     3481 gtttattatt aactgcattt ccgatacccc catccatacc cccttatatc ttagcgtgcc
     3541 cgatgcgccc tcgtgaatct ggcgcagagc ctcgcgttct gacaagtacg ccattttgcc
     3601 accttttcga ccgttcgg
//
LOCUS       DAXFWM010000111         3425 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000111 DAXFWM010000000
VERSION     DAXFWM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.111"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            98..3297
                     /gene="nalP"
                     /locus_tag="ACFI8B_002103"
                     /pseudo
     CDS             98..3297
                     /gene="nalP"
                     /locus_tag="ACFI8B_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
BASE COUNT          918 a          937 c          928 g          642 t
ORIGIN      
        1 gatataattc aaaatataaa aatgggttac atctggacat tacgaaattt ttattccttc
       61 gcctgaattc tattgtcaga ttcaaagaga cctcatcatg cgaaccacct caaccttccc
      121 tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt gcaacaacac tttctgcctg
      181 cttaggcggc ggcggaggcg gcacttctgc tcccgacttt aatgcaggcg gcaccggtat
      241 cggcagcaac agcagggcaa cgatagcgga atcagcagca gtatcttacg ccggtataaa
      301 aaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt cgggatgacg ttgcggttac
      361 agacagggat gccaaaatca aagcccccga atctgcatac cggagacttt tcaaacccaa
      421 atgaccaata ttaagaatat gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      481 cgcggggtag aggtaggtat cgtcgataca ggcgaatccg tcggcagcat atcctttccc
      541 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacaa attacaaaaa
      601 ctatacggcg tatatgcgga aggaagcgcc tgaagacaga ggcggtaaag acattgaagc
      661 ttctttggac aatgaggccg ttatagagac tgaagcaaag ccgacggata tccgccatgt
      721 aaaagaaatc ggacacatcg atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg
      781 cagacctgca ggcggtattg cgcccgatgc gacgctacac ataatgaata cgcatgatgg
      841 aaccaagaac gagaaataat gtctgcagcc atccgcaatg catgggtcaa gctgggcgaa
      901 cgtggcgtgc gcgtcgtcaa taacagtttt ggaacaacat cgagggcagg cactgccgac
      961 catttccaaa tagccaattc ggaggagcag taccgccaaa cgttgctcga ctattccggc
     1021 ggtgataaaa cagatgaggg tatccgcctg atgcaacaga gcgattaggg caacctgtcc
     1081 taccacatcc gtaataaaaa catccttttc atcttttcga caggcaatga cacacaagct
     1141 cagcccaaca cactgaccct attgccattt tatgaaaaga cgctcaaaaa ggcattatca
     1201 cagtcgcagg cgtagaccgc agtggagaaa agttcaatgg ctccaaccat tgcggaatta
     1261 ctgccatgtg gtgcctgtcg gcatcctatg aagcaaccgt ccatttcacc cgtacaagac
     1321 cgattcaagt tgccggaaca tccttttccg cacccatcgt aaccggcacg gcggctctgc
     1381 tgcagagata cccgtggatg agcaacgaca acctgcgtac cacgctgctg acgacggctc
     1441 aggacatcgg tgcagtcgga gtggacagca agttcggctg ggggctgctg gatgcgggta
     1501 aggccatgaa cggacccgcg tcctttccgt tcggcgactt taccgccgat acgaaaggta
     1561 catccgatat tgcctactcc ttccgtaacg acatttcagg cacgggcggt ctgatcaaaa
     1621 aaggcggcag ccaactgcaa ctgcacggca acaacaccta tacgggcaaa accgttatcg
     1681 aaggcggttc gctggtgtta tcggatatgc acgtcaaaac caaaggtgca ctgatttata
     1741 acggggcggc atccggcggc agcctgaaca gccacggcat tgtctatctg gcagataccg
     1801 accaatccgg cgcaaacgaa accgtacaca tcaaaaggcg gtctgcagct gggcgacaaa
     1861 ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg acggtacggc gattaccgac
     1921 ggcaagctgt acatgtcggc acgcggcaag ggggcaggct atctcaacag taccggacga
     1981 cgtgttccct tcctgagtgc cgccaaaatc gggcaggatt attctttctt caaaaatatc
     2041 gaaaccgacg gcggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa
     2101 ggcgacacgc cgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg
     2161 gcacattccg cgcccgccgg tctgaaacac gccgtagaac agggcggcag caatctggaa
     2221 aacctgatgg tcgaactgga tgcctccgaa tcatccgcaa cacccgatac ggttgaaact
     2281 gcggtcgccg accgcacaga tatgccgggc atccgcctac ggcgcacaac tttccgcgca
     2341 gcggcagccg tacagcatgc gaataccgcc gacggcgtac gcatcttcaa cagtctcgcc
     2401 gctaccgtct atgccgacag tgccgccgcc catgccgata tgcagggacg ccgcctgaaa
     2461 gccgtatcgg acgggttgga ccacaacggt acgggtctgc gcgtcatcgc gcaaacccaa
     2521 caggacggtg gaacgtggga acagggcggt gtcgaaggca aaatgcgcgg cagtacccaa
     2581 actatcggca ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcata
     2641 ggacgcagca catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt
     2701 gcaggcatac ggcacgatgt gggcgatatc ggctatctca aaggcctgtt ctcctacgga
     2761 cgctacaaaa acagcatcag ccgcagcacc ggtgcggatg aatatgcgga aggcagcgtc
     2821 aacggcacgc tgatgcagct gggcgcactg ggtggtgtca acgttccgtt tgccgcaacg
     2881 ggagatttga cggttgaagg cggtctgcgc cacgacctgc tcaaacagga tgcattcgcc
     2941 gaaaaaggca gtgctttggg ctggagcggc aacagcctca ctgaaggcac actggtcgga
     3001 ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag ccgtcctgtc tgcgacggcg
     3061 ggcgtggaac gcgacctgaa cggacgcgac tacgcggtaa cgggcggctt taccggcgcg
     3121 gctgcagcaa ccggcaagac gggtgcacgc aatatgccgc acacccgccg ggttgccggt
     3181 ctgggggtgg atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacacc
     3241 ggttccaaac agtacggcaa ccacagcgga caaatcggcg taggctaccg gttctgacgg
     3301 acagaaaaca gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa
     3361 ccctattcca attgcctgct tctattgttt caagacttct tccaaagatt cggcatcaat
     3421 cagac
//
LOCUS       DAXFWM010000112         3399 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000112 DAXFWM010000000
VERSION     DAXFWM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.112"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..375
                     /locus_tag="ACFI8B_002104"
     CDS             <1..375
                     /locus_tag="ACFI8B_002104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835217.1"
                     /translation="SSQSFSISKFCFLIALPDKAGLPPYCLSSHLLSCHRLLSPRFYR
                     FCQLRGFGGGNVEGGFDGFGGIGVDPYSSANGNTTDGVCFSAVCRFDQGAAVEGAGVG
                     FLKNSVLNILLQMKERPSEKQA"
     gene            complement(411..956)
                     /locus_tag="ACFI8B_002105"
     CDS             complement(411..956)
                     /locus_tag="ACFI8B_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC3835218.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLNNEVDNLSDMKRKPFAW"
     gene            complement(1077..1349)
                     /locus_tag="ACFI8B_002106"
     CDS             complement(1077..1349)
                     /locus_tag="ACFI8B_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835219.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            complement(1597..2016)
                     /locus_tag="ACFI8B_002107"
     CDS             complement(1597..2016)
                     /locus_tag="ACFI8B_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC3835220.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            complement(2013..2750)
                     /locus_tag="ACFI8B_002108"
     CDS             complement(2013..2750)
                     /locus_tag="ACFI8B_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC3835221.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEVSDGIGNIQTLISNRIHPFYSDGKWIKAEDLKAGSRLYSESGRTQTVRNTIV
                     KPTPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            complement(2881..3258)
                     /locus_tag="ACFI8B_002109"
     CDS             complement(2881..3258)
                     /locus_tag="ACFI8B_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC3835222.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            complement(3289..>3399)
                     /locus_tag="ACFI8B_002110"
     CDS             complement(3289..>3399)
                     /locus_tag="ACFI8B_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC3835223.1"
                     /translation="RYERFRVMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
BASE COUNT          936 a          732 c          620 g         1111 t
ORIGIN      
        1 tcttcacaat cgttttccat atctaagttt tgttttctta ttgccctgcc ggataaagca
       61 ggtctgcctc cgtactgtct atcctcacat ttactttcct gccatcggct tttatctccc
      121 cggttttacc gtttttgcca acttcgcggc tttggcggtg gcaatgttga aggcggcttc
      181 gacggtttcg gcggcattgg ggttgacccg tactcatccg caaacggcaa cacgacggac
      241 ggtgtttgct tttcagccgt ctgccgtttt gaccaaggtg ctgccgtgga aggagcaggt
      301 gtaggatttt tgaagaactc agttctcaat atcctgcttc aaatgaagga aagaccatct
      361 gaaaaacaag cttaatttca gacgaccttt aatcaaattt aagaaagccc ttaccaagca
      421 aacggtttcc gcttcatatc cgaaaggttg tcaacttcat tattcagcaa gaactgctca
      481 aaagcattcc aacctttctt ttccaccaat tctgcttcct gtttatacaa ggggacaagc
      541 aaagggaaaa cgatattgta gtgttcgcca taacagacct gaaaatcatc atcgaaataa
      601 aatggggcgg aaacatacag tgcatccatt ttgctacctt cgataattac cctgggtaat
      661 tgaataattt cacctcttaa aataggttgt ctattgtcaa tcaaatattc agccactcct
      721 gatagaaaag ctgcaatatt ttctttattc cattcttcag aacaggtaaa aattaattca
      781 aacctactct tatagttcaa gtcatatcga ttcaagccta aagtagaaaa agtagttacc
      841 cctttatatg gttgtgatgg gaagacggaa acgctgagat gataacgtcg atccaagcat
      901 tttataccgc tttcgatttc acccaaataa tgttctaaat gttcaactat aatcattttt
      961 tcttaaagat ttcatcccta tcccggcaag acgtaggctg ttttgggctc caataccgac
     1021 ttttttattg acggaaatgc cgtctgaaaa ataaagcggc atcttgattt tgccgtctaa
     1081 ggactttgca tccattcctc tttgctgatt ccaaattcaa attcagcctg ttcaaatgcc
     1141 gaatctaatg tgtcatgata agtatccgtt tgttcctgac cggtctcatc caaataaaac
     1201 aaataaaatc ccgggtcatt ttcatattga acaatactta aagcaacagg tttgggaagt
     1261 atccccatcg gggtacgatg aatcgttcta ataggaaaat ctttattttc atctaagatg
     1321 actttgaata attcttttac actcatcatt cttcaatccc gaataattgt taccgcacta
     1381 ttttctatta cacgtccatt ggctttgtag ccgtctgccg ttttgaccaa ggtgctgccg
     1441 tggaaggagc aggtgtagga tttttgaaga acttagtttt caatatcctg tttcaaatga
     1501 aggaaatagg tcgtctgaaa attaaacacg gtttcagacg accttttaat cgttaggttt
     1561 acaatctaac gtaatccatt cttacttata gatcgattaa gagaagccca aacaagcttc
     1621 cctgtagctg aggatatttg cttcctcata tctatttaaa attcctttta aatcagaatt
     1681 atattttttg ggcggaaaat taaccgataa ataattctct ccattttttc tgattttacc
     1741 actgtgttct tctatggctg ccaataaatc ctttgggaaa tgatccaaaa caacgatgtt
     1801 tatcgtggta tttcctgaag cttttatcaa gtggtcgaaa aacagcatct tatcctcacg
     1861 ttctatggca acaatgtcgt ttaaagccaa attcggtgcg aaaagaggaa tctcttgaat
     1921 aagaaaattt ccatcaggca ttatgtttcc tgacagtatt tcttctgcaa tattgctttc
     1981 caaatcgtaa tagacgacag taagtttcga tttcatttgt tactcttctt aatgttgcag
     2041 cctctgcata atacctgccc gtttttaggt gtaccttgac cacccttaga tttagggatg
     2101 acatgatcta cttgtctttc atttgaagta ggagaaatat tttttataga ttgtttggct
     2161 ggaattgttt caataccgca attagcgcac ttaacttgac cattattttg aatcctattc
     2221 aaatcaataa cggcttcttt tcctgcttta gtaaagtctt ttcctgcccg atcgttttta
     2281 ctaagccgtt ctgcgcgaag ttttgctttc tctaaattat ttgaaccgcc atacggacaa
     2341 tcattatgaa cccaaacccc ttccgtttcc gcctgattac ccttgacgaa gtaggtatgc
     2401 cagtcggcaa cggtcagatt gtaggctttg agcggtgttg gtttgacaat ggtgttgcgg
     2461 acggtttggg ttctgccgct ttcggaatac agcctgcttc ccgctttcaa atcttccgcc
     2521 ttaatccatt tgccgtccga ataaaacggg tggatgcggt tggaaatcag ggtttggatg
     2581 ttgccgatgc cgtctgaaac ttcaatgtaa acggtttctt ggtacggatt accgtatcgg
     2641 gcggtaacgg gtttgtatcc cgtctccccg cttgcctcgt ccttggaaag gacgcggtct
     2701 ccggcttgaa tatgggcgat ggctttgtag ccgtctgccg ttctgaccaa ggtgctgccg
     2761 tggaaagagc aggtgtagga tttttgaaga acttagttcc caatatcctg tttaaaataa
     2821 ggggaaggtc gcctgaaaat taaacacggt ttcagacggc attttgattg ccgggttttg
     2881 ctattttttg ttgtaataat caaattgcac gttgactatg tctttctcgg taaaaatata
     2941 acggagcatt gttttaagcc tttcataacg ttcgttaatt cccacgctat caggtaacca
     3001 aggggaagct ttaatttcaa aaagtttcca atttggaacc attaaaaact caataatggt
     3061 accgattcca atcacgatat cccttggtat atccatcgga taaggatatt ttctcctaac
     3121 ctcgattaaa tcattctcca acttccaata ttcttcatca tcccacaccc cgtcatcata
     3181 ccatttgcca ataaatgaat tttcgtcata cccctcaaaa taaggaacgt ttcttataat
     3241 atccttgaac tcacacataa taatgtatct ccaatataat taaacttttc atctcaatct
     3301 acctttacta tgttgtattg gaaagtaaaa aaatttccag tcctctacat ctagatcagt
     3361 aaaaatataa cggagcatta ctctgaacct ttcataacg
//
LOCUS       DAXFWM010000113         3364 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000113 DAXFWM010000000
VERSION     DAXFWM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.113"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(158..571)
                     /locus_tag="ACFI8B_002111"
     CDS             complement(158..571)
                     /locus_tag="ACFI8B_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835224.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(568..1029)
                     /locus_tag="ACFI8B_002112"
     CDS             complement(568..1029)
                     /locus_tag="ACFI8B_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3835225.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1046..1483)
                     /locus_tag="ACFI8B_002113"
     CDS             complement(1046..1483)
                     /locus_tag="ACFI8B_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835226.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(1596..2312)
                     /locus_tag="ACFI8B_002114"
     CDS             complement(1596..2312)
                     /locus_tag="ACFI8B_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC3835227.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            2381..2617
                     /locus_tag="ACFI8B_002115"
     CDS             2381..2617
                     /locus_tag="ACFI8B_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3835228.1"
                     /translation="MAMSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRC
                     VQIEKLTGGAVNRKDLRPDDWHLIWPELAGDQPK"
     gene            2697..2852
                     /locus_tag="ACFI8B_002116"
     CDS             2697..2852
                     /locus_tag="ACFI8B_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835229.1"
                     /translation="MKKQDRNRLSKKDRRLIKKAMLKAAAKGCDEVYRIAPGLKDGFE
                     LLGKQPD"
     gene            complement(2829..3017)
                     /locus_tag="ACFI8B_002117"
     CDS             complement(2829..3017)
                     /locus_tag="ACFI8B_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835230.1"
                     /translation="MPSDLSFILAKELIRSGSIRLKGSTAKGQAGELAVFIRTLHQKP
                     EESEPNTDNEYLIGLLSK"
     gene            3190..>3364
                     /locus_tag="ACFI8B_002118"
     CDS             3190..>3364
                     /locus_tag="ACFI8B_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3835231.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPRMKAGG"
BASE COUNT          849 a          731 c          843 g          941 t
ORIGIN      
        1 tataactatt acctatattt aatcaatagg caatagttat attttcatcc atatatactt
       61 attgaattga tttttagaag aaaaaagttt gaaaaaaaac cgcccgaatg gcggtgtatc
      121 atgaaaaaga aaaccgccct agggcggttc ggtattgtta tgagtggtaa ggaagccaag
      181 aaaaagacaa aggacggctc tcggagagaa tatggttgtg tgttgtcatt cttacataaa
      241 tgcggtcgtt gtctgaaacg ggtagtccgc tgattgtttg gtgtagcatg aatgacacgt
      301 tttcttctgt atcactgccg tcatatgatg gaatctcaag tgcagagatt acatcatcgt
      361 tgcggacgat ttccagaaaa atttgaccgg ttttcatagt ctgtatatcc atgccttcgg
      421 cggaaaatga aacagacagg cacaggcggc ctagaatggc ctgcggtgtc ggaatatggc
      481 atatatcggg gaatatacct atcaggctgt atttgtcatt ttccgcatgg cggataattt
      541 catcgcaata gtggacattt aagtgaatca tgcctgctcc atatattcgt accgccgttc
      601 gaacgctgcg cgaacttcaa gcggggaaac gcctaatgcg tttgccaatt tttgtacagt
      661 cttatcttgc agggattgtt tgctgttttc gatgcgggac agatagggtt gaggcaatcc
      721 tgcggcggtt gccaattcgg actgggtaaa cccctttttc atccttaagc tgacaaatgt
      781 ttccccgcct gctttgaggg ctattttatc ggcaattctt gctgccgccc tgtccatcgc
      841 tgctttgcgg cgcggatttt tccgcacatg ctcaatatgc tctgcggcag gggtagtgtt
      901 agggcttatc ggtttgacgc atttaatctg cccgtttatc cgcaggcatt ttatgtgggt
      961 gacggggatg gcggatgtag gtgttgatac tgtgcaggcg gccacggcaa cagtgagtgt
     1021 tgcacccatt ttgaaccatg tgccgttagt tggagtacag ttcttcatag tctttgataa
     1081 tccgttttgt gattgggtgt tctgcctgat agtcaaattc gtctgtcttc ttgtttacaa
     1141 cagccaatat atctatgcgg cggatatacg tgccgtttgg ctgtttttca tggcatggtg
     1201 cgtaaattat tctgtatccg gcggcttctt cgctgtcaaa tcggacgcgt aaaactttga
     1261 tgtcttttcc ccataatgac aatatgggtt tcacttccaa gtcaatcggg cctatgggtt
     1321 cgccatattc cctgaagtat ctgtttttgt ataagccgtc aaaaatagca gagtccgcct
     1381 gaatcatcgc aatgacattc tcgagataac cgaccgcttc ttcgtctttt tcaaagaggc
     1441 ggtctaaatc ctgctcggcg ttaaagtgga cggtcagctc catgagtgcc ctttttatat
     1501 atatattata tctttttagt tataccgcgc aatatgcggt aataaatcag ttgttcaagt
     1561 attttaatcc ggcacgctcc accagaagac gcggtctaga actggctcaa acgccatgcg
     1621 ccgcgtatgc gtccgatgat gcgtacggcg tttaaatctt cgccgcgcac ggtttcggtt
     1681 cggtatgagc tgttgtcgct gatgatcatc aggccgccgc cgacggtgga ttgcagccgc
     1741 ttggccttaa ggccgtctat ataccaaagc aggtagaggc cgtcgccatc gaaggcttcg
     1801 acggcggtat caacgaacat tacgtcgccg ttttcgatgg tgggctccat gctgtcgcca
     1861 cgggctgtaa tgacttggat tttgttgagg tttccgccca gtttctcccg cgcccatgcg
     1921 gcagcgacgg ttacataatc cacaacctcg atatagtggt cgttaatcgt gcctgcgccg
     1981 caggtcgctt cggcatttaa gcgggggaaa cgtatgctac tttgttcatt cgattttaag
     2041 actctgaatg tttcaatgcc gaaatggctt ggcgttacta cgtctgagaa ataatcaatt
     2101 aatttatcta gatgtttttt atctattcgt ccatttttta tccaacctga aacgcttggc
     2161 tgtttcaccc caaaatgatc ggcaacctcc ttttgactga cattttttct cttaatcgct
     2221 tctgatattg cttgtcctaa ctgttcgcct gaaaacattt tgatctccga attaattgcg
     2281 cataagcatt ggtaatcgat aataaaaggc ataacctata aaaggcaata gttgtattta
     2341 atataagtat tagctataat gaagctattt aattgagtaa ctggctatga gtatccaaaa
     2401 agcagttgat tattttggta atgaatcccg acttgcacgg gcgatcggag ttaaacaacc
     2461 gacggtgtgg gcttggaata aaaaaggaac gccgcccccg atcattcggt gcgtgcagat
     2521 tgaaaaatta accggaggcg cagtgaatcg aaaagactta cgtcctgatg actggcatct
     2581 aatctggccg gaattggcag gcgaccaacc caaataaaaa gcccgtcggg tcagatgtta
     2641 attgatataa ccgaattgaa gcggaagtca tccgcaattt accggaaagg aaaaaaatga
     2701 agaagcagga cagaaaccgc ctgtcgaaga aagacagacg gctgattaaa aaggcgatgc
     2761 tgaaagccgc cgccaaaggc tgcgatgagg tttacagaat cgcgccgggg ttgaaagacg
     2821 gctttgaatt acttggaaag cagcccgatt aaatattcgt tatcggtatt tggctccgat
     2881 tcttcgggtt tttgatgaag tgttcggatg aataccgcca attctccggc ttgtcctttg
     2941 gccgtactgc cctttaagcg gatagaaccg ctgcggataa gctctttggc gagtatgaag
     3001 gataagtcgg acggcatttt tttactccgt cggccgttgt gtggaaaccc ggttgcaacg
     3061 gggtgacggc aaatcggaaa gacggttgac cgcccggaca gacgggcggc cgataaagaa
     3121 aaacccgcac ggggcgggta atccccctga attgcaggga agcggttcag gtaacggcga
     3181 aaggcgatta tgaatcaaaa acaaacgcaa tgcaaacaaa ttgtcgatta catccgtaac
     3241 aagggatgca tcacatccct tgaggcttat cagaacctga aggtgacgca gcttgcggca
     3301 cggataaccg acttggaagg caggggcttc gtgtttgcca agccgcgcat gaaggcgggc
     3361 ggcc
//
LOCUS       DAXFWM010000114         3298 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000114 DAXFWM010000000
VERSION     DAXFWM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.114"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..242)
                     /locus_tag="ACFI8B_002119"
     CDS             complement(<1..242)
                     /locus_tag="ACFI8B_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835232.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKN"
     gene            complement(246..1139)
                     /locus_tag="ACFI8B_002120"
                     /pseudo
     CDS             complement(246..1139)
                     /locus_tag="ACFI8B_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1157..1501)
                     /locus_tag="ACFI8B_002121"
     CDS             complement(1157..1501)
                     /locus_tag="ACFI8B_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835233.1"
                     /translation="MNLENYENILIKLLFHHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKEHFGSFMCWINTNLMEFEGVFFNDPLPD
                     NMIINNFFEIND"
     gene            complement(1900..2301)
                     /locus_tag="ACFI8B_002122"
     CDS             complement(1900..2301)
                     /locus_tag="ACFI8B_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835234.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(2305..3045)
                     /locus_tag="ACFI8B_002123"
                     /pseudo
     CDS             complement(2305..3045)
                     /locus_tag="ACFI8B_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          937 a          667 c          554 g         1140 t
ORIGIN      
        1 tgatttttta attgttcgtt gtctaatata gcaaaaggta attttgcaca caaataacca
       61 ataactttgt ttttatgaaa aaaaactatc caattttcaa ttccttcatc ccaatcaaaa
      121 gtaaaattcg ttccttctat gataaattta gcacaagata ttaaatctaa cttaacccaa
      181 gtggatttat acagactcaa tgctgatgaa gttataatat tatctaagtt ataaattttc
      241 ataaatcaac gttccttacc tggtgttcca tgcacaggtc taatcccatc tttattcggc
      301 tttctacgat aaatgttaat tatatgtgtc ggttttccgt ctgtatagcc aactacgtcg
      361 ttcgttggaa cttttgcctt attttgacta tcccataaat tatgtttatc tgcatatcta
      421 gcagcatcta atgtagcttt ttccgcatca acatctgatt taaataaact tttgctatct
      481 atcttgtcta gactagatgc atcaggtttg tgtcgtacca tactgagagg gtcagtaata
      541 tatttgccat ctgtaatagc tgatccacca ttataggtag tatgcgtata atttttttgt
      601 ggaacgagac tattttgcaa agattgttgt gtagaaacgc cagctttgcc attaggagta
      661 gcttgcttcg ctggcttatt cccacctgcc atctccccat ctaaagtctg catatggcgg
      721 gcagcaggtt ttttatagcc ggtattttta acccaatccc atttagtcgg gttaagttct
      781 acttttttac ctctccaaac cgtagttgcg gcctctgcta cggcaagggc agtttgggct
      841 gttgctgtta tattcggatg ggcatcagcc cattgtctgg cggaattgat gccgtctttt
      901 accgcaaaag cactgtcctg taataggctc gcggcagcaa gttgtgcttc cggacttaat
      961 tttcctaaat cattaatacc ttgtagagtc tgctgagcgg ctgtatctgt gaccgggctt
     1021 actgcactgt ctgtaattgc cccaatccct actccttgaa aacccattaa aaaaggatta
     1081 accgcacctt ggacgatgcc gcgaatatca tccatacggt tagcccacca atttttattg
     1141 gtttattggt atatcgttaa tcatttattt caaaaaagtt atttattatc atattatcag
     1201 ggagcgggtc gttaaaaaaa actccctcaa attccattaa attagtattt atccagcaca
     1261 taaacgaacc aaagtgttct ttccctgagt gtaaatattt aaaattaaat tcaaaataag
     1321 tttttttgct tagaaaattt aatggttttt ctatagtgaa aaaaaagccc ttactcgttt
     1381 tagttcgaga tactatttta aataagtttt tattctcaat aaaataagaa tattcattta
     1441 ctaagttgtt atgatgaaaa agtaatttta ttaaaatgtt ttcataattt tctaggttca
     1501 tttctagctc aatttaaaat gatttcattg ttgccgtacc aatgcgaaat ttaccatcga
     1561 caaaatttcc cttcactgat acaggtaccc catcgcagag atgtaggtcg gatatttgta
     1621 tccgacaaaa atatttgacg cgtttatcgt ttccgaaatg ccgctgttag aaaatgtcgg
     1681 attcgagaat ccaacctacg gctatatccg taaagtctga ccattgttca atgcggaagg
     1741 cgcgaatatt gcggacggaa tgtagaaacc aatatactta attgccatgc cattctcatc
     1801 aaaatacctg tgctgtttaa agtgcgtggt tcattacacg agtaatttta ttttcaggct
     1861 gcctgaaaga aagataggca acttattact catcttacgc tagctctcaa taattgaata
     1921 cgttgcacta gaaccaatta tttctatgaa tgtttgctca aacataaaaa tatagtattt
     1981 aaagaattct ctattcaaag gaaaataact ttctttcgat atccatgccg cataacgctc
     2041 atcaagaagt tcgaaaaaag attgaccacc attttcatga agcaaagctc tatctgcatc
     2101 ttccgaaatt aaatccataa aagtatctat aaaatacgat tcatcaacaa ccctaaaatg
     2161 aataacatcc tcaatagaaa tttttatggt tttacccgta tttctataac caactgctat
     2221 aacttctact ttatcaaagt ttattgctgc attatcaaaa tagaaatagc cagttgggaa
     2281 atctaagctt tttaatttca taatttatcg tgtatttcca tatctgattt ttatttttcg
     2341 gttattacta attgttattt caagtgttgg ttctccacca ctgctatcat tacgaacatt
     2401 aaccgcacgc ccatcaggta aattgcccgt tatcgttcca ttaggacgtg tttgtacatt
     2461 tcgaacattc agactattaa aatcatttag tgcgtcattg taatccccca ctttatcata
     2521 ctgaacagcg ggaccttttg ttttactcct ttgagtagta ttgctcaaaa tgtctttgat
     2581 ttgtaaatca ctgcgcacct taccggcttc tatacgagct gacacactac tcgggctata
     2641 ggggtcgcta tcaatcgcag gattaccacc gctggttttt tgtccgctag tctgtttccc
     2701 aacctgctgt actgtattgc caatttcatc taccgtacct aacgccttag tctgcatatg
     2761 gcgggcagta ggttttttat aaccgttatt tttaacccaa tcccatttgg ccgggttaag
     2821 ttctactttt ttaccgcccc aaaccgtacc tgcagcctct gctacggcaa gggcagtttg
     2881 ggctgttgct gttatattcg gatgggcatc agcccattgt ctggcggaat tgatgccgtc
     2941 ttttaccgca aaggcactgt cttgtaatgc ggttgcagcc gcaagttgtg ctgcgggact
     3001 taaatttcct aaatcgtgaa taccctgtaa agtttgctgc gcagaaatgt aggtcggata
     3061 tttatttgta tccgacaaaa atatttgacg cgcctatcgt ttccgagatg ccgctgttgg
     3121 aaaatgtcgg attcgagaat ccgatctacg gctgctgact attttcttgt ttatataatt
     3181 ctacaactgc aaactactat caaatctttt tatctctggc tctacactca gcaagaatat
     3241 ttttatttta caaaacatag gctagttatt tgagtatatt gtcaaattct aatttgtt
//
LOCUS       DAXFWM010000115         3154 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000115 DAXFWM010000000
VERSION     DAXFWM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.115"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            121..669
                     /locus_tag="ACFI8B_002124"
     CDS             121..669
                     /locus_tag="ACFI8B_002124"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3835235.1"
                     /translation="MNAKFIQADIFELQPETLEKELGLKKNDDDLILIGCSPCQYWSV
                     IQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGILSRMKESGLDNFIKLLEEKG
                     FTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSSIRANGLRYAMFWEWKTAFPK
                     LWQDIKTRRILCIVVRDYPISI"
     gene            708..1037
                     /locus_tag="ACFI8B_002125"
     CDS             708..1037
                     /locus_tag="ACFI8B_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3835236.1"
                     /translation="MAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSI
                     SNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRA
                     IVDNIEC"
     gene            1051..1908
                     /locus_tag="ACFI8B_002126"
     CDS             1051..1908
                     /locus_tag="ACFI8B_002126"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3835237.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFRLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVKPNTENGTTIT
                     LTELTKKQGYPLDNYVGHLSRLFDFPAQDFKIKVSLNGSEPRIIDGNLKYNLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHF"
     gene            2098..2694
                     /locus_tag="ACFI8B_002127"
     CDS             2098..2694
                     /locus_tag="ACFI8B_002127"
                     /inference="COORDINATES: protein motif:HMM:NF021057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC3835238.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            2700..3122
                     /locus_tag="ACFI8B_002128"
     CDS             2700..3122
                     /locus_tag="ACFI8B_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC3835239.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
BASE COUNT         1092 a          515 c          698 g          849 t
ORIGIN      
        1 aacagtaagt taaaggcggt tgattttttc tgtggcggag gcggaatgag ctatggaatg
       61 taatccgctt tagccgggat agattacgaa cttagctgca aagaaacata tgaagccaat
      121 ataaatgcca aatttataca ggctgatatt tttgaattgc agcctgaaac cttggaaaaa
      181 gaactcggct taaaaaagaa tgatgatgat ttgattctta taggttgtag tccttgtcaa
      241 tattggagtg ttattcaaac agacaaaaga aaatcggaaa aatccaagag tttgcttttg
      301 gagtttcagc ggtttgtaga atatttcaat cccggatatg ttgttgtaga gaacgtaccg
      361 ggaattctga gcagaatgaa agaaagcggg cttgataatt tcataaagtt attggaagaa
      421 aaaggattta ccgtccattt cggtattcac aatacggctg attacggaat tccccaaagc
      481 cgtaaaagat ttacgttaat tgcaaacaga ataaccaaaa aaagctggaa ccagtcaagt
      541 attcgggcaa acggcttacg gtacgcgatg ttttgggaat ggaaaacggc tttcccaaaa
      601 ttatggcagg acatcaagac gagacggatt ttatgcatag ttgtgcggga ttatccgata
      661 tcaatttgaa acgattggct ttgataccta aaaacggagg aaaccgtttg gcttttgcgc
      721 atattcccga attacagctg gaatgtttta tcggaaaaga caatagtttt aaagacacgt
      781 tcggtaggct atggtgggat aagcccgcac ctaccataac gaccaaattc tttagcattt
      841 ccaacgggcg gtttgcccat cctgaagaag atagggcgtt atccctgcga gaaggtgcaa
      901 cattgcaatc gttccctcgt aactatgtct ttaaagcggg cagtagggac aagatagccc
      961 gtttgattgg taatgccgtt cctccgatgt atgcggaaaa aataggcagg gcaattgttg
     1021 ataatatcga atgttagaaa aggagaaaac atgtctgaag aaaaattgaa aatgagtttc
     1081 gagccaaccg taatcgaaca tttgggtgta aagatgtatt cgcacactgt tcctgcgatt
     1141 gccgagttga tagcgaatgc ctacgatgca tgtgctacgg aagtggaagt taggttattc
     1201 gataaaccgg agcataaaat cgttattaaa gataatggca taggaatgag cttcgatgaa
     1261 atcaatgatt tttatttgag aatcggtcgg aacagaaggg aagaaaaaca agcctccccg
     1321 tgcggaagaa ttccaacggg taaaaaaggt cttggtaaat tggcattatt caggcttggc
     1381 aacaaaatcg aaatctctac tatccaagga aacgaacggg ttacttttac tttggattat
     1441 gcagagatta aaaaaagtga gcgtatttat caaccggagt ttcagaaaga gtctgttaaa
     1501 cccaataccg aaaacggaac aactataact ttaaccgagc tgacgaaaaa acaaggatac
     1561 ccgttagata attatgtggg gcatctttcc cgtttatttg attttccggc tcaggatttt
     1621 aaaatcaaag taagcttgaa cggctcggaa ccaagaatca ttgacggaaa cctaaaatat
     1681 aatcttgtta ccccacaatt cgaatgggaa taccaggatc tagcaaccaa tatttcatcg
     1741 ttatcttcaa aattcgaaca gtatgaatac agcggattaa tacaaggtaa gttcattaca
     1801 acggaaaaac ctttaaagaa taatatgaaa ggtattacct tgtttgccaa cggcagaatg
     1861 gtaaatatgc ccgagttttt cactgatagc gaatccagcc atttctaatc ctatctgaca
     1921 ggatggttaa gtattgattt tatagataat gatggtgaag atttaatcgc tacagatagg
     1981 acatcattgg attggaagca tcctaaaact tcaaaattac gggttttcct tggggaaatc
     2041 gtctgagcca tcgggcaaga ttggagaaaa agaagaaaaa aagagaaaga ttacgaagtg
     2101 aaaggtgaaa gcggagttga tatcgaaaat tggaaaaaca aattgccaga aaaggaaaga
     2161 gaaccggtag aagttatttt gaatcgtttg gaagattcgg agcttacaaa taaagaacaa
     2221 gcggaagtaa tctctgcctt acatagtatt attccagaat atccttacta tcattggcgg
     2281 catttgcatc aggatttgca tactgcctgt aacgattttt acaatgaaaa gaaggattat
     2341 ttaagtgctg caatcgaggc agttaaagtt ttcgaagata aggttcagaa gcagacaggt
     2401 cttcatagca tagacggaag agaattgatt gaaaaagcct tcgggagtaa aaaatctatg
     2461 cttctattaa cgaataacaa aacgcaggcg gaacaaaatt tagaagatgg tcttgaacag
     2521 cttgcatgcg gaacatggac tggatttaga aatcctgtgc aacatgagtt aagagcaaat
     2581 ttatctccta gtatttttaa tgacaaggat gctttggatt taatcagctt agtttcatat
     2641 ttattgagaa aagtggaaca aacaaaaaaa cgagctaaac ctacatcgcc ttaaatttta
     2701 tgaccgatat tttcactcca tccaaacgtt ccttcgtgat gtcaaaaatc catagcaaag
     2761 aaaccaaacc ggaagtattg gtaaggaaat ttcttttttc ccaaggtttc cgttatcgga
     2821 aaaacgataa gcgctatgca ggcaaaccgg atatcgtttt gcctaaatat aaaacagtcg
     2881 tgtttataca cggctgtttt tggcacgggc attcttgtaa taaaggacat atcccgaaaa
     2941 gcaatatgga tttttggttg gaaaaaatca caaaaaatcg cgaacgtgat attaaaaatg
     3001 aaaccgagct ggaaaagatt ggtttcaaag tgattgtcgt ttgggagtgc gaattaaaaa
     3061 ataaagctat ttgtagggag cgattaaatc ggttggtcga ggaaataaaa gatgccgtct
     3121 gaaaaaatca gacggcatct tcctttaatt aaga
//
LOCUS       DAXFWM010000116         3083 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000116 DAXFWM010000000
VERSION     DAXFWM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.116"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(188..625)
                     /gene="rnhA"
                     /locus_tag="ACFI8B_002129"
     CDS             complement(188..625)
                     /gene="rnhA"
                     /locus_tag="ACFI8B_002129"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC3835240.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            738..1607
                     /gene="tehB"
                     /locus_tag="ACFI8B_002130"
     CDS             738..1607
                     /gene="tehB"
                     /locus_tag="ACFI8B_002130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC3835241.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            complement(1665..2471)
                     /gene="thiD"
                     /locus_tag="ACFI8B_002131"
     CDS             complement(1665..2471)
                     /gene="thiD"
                     /locus_tag="ACFI8B_002131"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC3835242.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2531..2683
                     /locus_tag="ACFI8B_002132"
     CDS             2531..2683
                     /locus_tag="ACFI8B_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835243.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            complement(2899..2974)
                     /locus_tag="ACFI8B_002133"
     tRNA            complement(2899..2974)
                     /locus_tag="ACFI8B_002133"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2938..2940),aa:Glu,seq:ttc)
BASE COUNT          673 a          762 c          911 g          737 t
ORIGIN      
        1 ctgctgtccg caacttttcg tcattcccgt gaaaacggga atctagaact tttaaatttt
       61 cagacggctt ttgaatattg ccgttgcccg acgttctgga ttcccgcctg cgcgggaatg
      121 acggtggagg acgatgccgt ctgaagccca ttagcggttt cagacggcat ttttgtcggt
      181 gcggcaatca agaaaactgc gccgcgccac ggtttgccaa atcgtcggcg cgttcgtttt
      241 ccgcgtgtcc cgcgtgtcct ttaacccaag tccagctgac ttgatgctgc ccgaccagag
      301 cgtcgagttc ttgccacaag tcgtcgtttt tgacgggctg tttggcggcg gttttccagc
      361 cgttgcgctt ccaaccgtgt atccagtttt ccatgccgtt tttgacgtat tgcgagtcgg
      421 tgcagatgat gacggtgcag cggcgtttga gcgatttcag cccttcgata acggcggtca
      481 gctccatgcg gttgttggtg gtttgcgctt cgccgccgaa aagttctttt tcgcggctgc
      541 cgtagcgcat taatacgccc cagccgcccg cgccgggatt gcctttgcac gcgccgtccg
      601 tgtagaggta aacgggtgtg tccatatcgt gcctttgtgt ggtgtccggc gtgattataa
      661 gcggtttgtg cgtcgtgccg tctgaaagat tgcggcggat tgaaataggc atataatgtg
      721 caactttatt tttgaatatg aaggagcgga tagtgggtca aagtggcgag ttgttttgtt
      781 tcggacaaat gcctgtatgg aaagtggaaa acctgccgga agttttattg tcgggttatt
      841 cgtccgagga aggggaatgg gtctgcctga atgtgttgca gggcgatgtc gaagtccgtg
      901 caccggacgg ggcggcagag gtttggtcgg cggaaagcgg cgattgcgtg tttgcgccgc
      961 agcaggtgtt ttcggtcaaa ccgaaaacgg acgatgccga aatccgtttg tcgctgtatt
     1021 gcgcggcggc agactatttt cacaaaaaat acgggatgag tgccacgcat tctgcggtcg
     1081 cggcggcaca ggataccgta ccggcgggca gggcgttgga tatgggctgc ggacaggggc
     1141 gcaacgcgct gttcctgggt ttgaaggggt ttgatgtaac cgctgccgat tgcaatcccg
     1201 ccgcgttggc aaacgtggcg gaactggcag aggcggaggg tttgaacgtc cgcacgctgg
     1261 aatatgattt gaacgccgcc gcccttcagg gcgagtttga ttatattgtg gcaacggtgg
     1321 tgctgatgtt cctgatgccg cagcgcgtgc ccgacgtgat tgccgatatg caggcgcata
     1381 cggcggcggg cgggtacaac ttaatcgtat cggcaatgga tacggcggat ttcccctgcc
     1441 cgatgccgtt ccccttcaaa tttaaagagg gcgagctgaa ggattattat cgggattggg
     1501 agctggtcga atataaagaa gaattgggcg cgatgcacgc caaagacgaa aacggcaacc
     1561 cgatacggtt taaatttgta actatgctgg cgaaaaaacc aggataagcc gcgccggacg
     1621 atgccgtctg aacccgaaaa ggcgttcaga cggcattttt acgtctagtc ccgatacgcc
     1681 caatgattga ccggcccgtg tcccgcgccg atttccaaag ggtttgagat tgccgccgtg
     1741 atgtaggcct tggcagtctg tacggctttg caaacgtcta agccttttgc caactcggcg
     1801 gtaatgcagg cggaaaacgt gcagcccgtg ccgtgcgtgt gggcggtcgg aaagcgcggg
     1861 ctgtccaatt ccagcgtttc gttttgcgta aacagccaat ccgtgcagcg tccgcttgtg
     1921 ctgccgttca aatgtccgcc tttgataatg acatttttga caccgtaatc aagcaggatt
     1981 tttgccgcac gttccgcatc tttacggttt tcgatatgca cgcccgtcaa ggcttccgcc
     2041 tcgggcaggt tgggggtcag gatgtccgtg tccggcagca gcaggcgcgt cagtgccgca
     2101 acggcggaat cctgcaacag cggcgcaccg cctttggcaa tcatcacagg gtcgagtacg
     2161 cgcctgccaa agctgcagtg tttcagcttg tcggcaacgc attcgatgat ttccgccgtg
     2221 ccgagcatac cgattttgta ggcgcggatg tcaaagtctt ccctgattgc ttggatttgt
     2281 gcggtgatgg tttcggtcgg gacgagatga accgccgaca cgcccaaggt attttgcgcg
     2341 gtaacggcgg tgatgacaca cgttccgaac acgccgcgca tctgaaacgt tttcaaatcc
     2401 gcctgaatgc ccgcaccgcc gcccgaatcc gaaccggcga tggcaagcgt ctgcacaaaa
     2461 gagcctttca tttgttcctc cttaatattg cgtccgataa aaagggctac tgcctgtaaa
     2521 aaagcaggca atgccgtctg aaaaccgcag cgcggggttt gtggtgcatt attatgtcgt
     2581 attgcaggac aaggcacagg gcaaggcagc ggggtgcatc gtagcaactg ataatgaaaa
     2641 aagagggcga tgccgtctga accgcgtgtt gcggcgagta taaaccggca cgcttcattc
     2701 gtcattcccg caaaaacaga aacctaaaat tccgtcattc ccgcgcaggc gggaatccgg
     2761 accttagaac aacagcaata ttcaaagatt atctgaaagt ttgaggttct agattcccgt
     2821 tttcacggca atgacgaaaa attgcgggaa taacgattca taataaaaaa cctgcttgaa
     2881 acaagcaggt ttttgaattg gcacgcccac ggggattcga accccggttg ccgccgtgaa
     2941 agggcagtgt cctaggcctc tagacgatgg gcgcgtggaa acggacatta tacatactta
     3001 tagaaattta gcaatacgat gccgtctaaa cgaatgtcag ggggcgtttg tctgaccgta
     3061 tcacaagata aaaaacattg gaa
//
LOCUS       DAXFWM010000117         3063 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000117 DAXFWM010000000
VERSION     DAXFWM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.117"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            658..1455
                     /gene="truA"
                     /locus_tag="ACFI8B_002134"
     CDS             658..1455
                     /gene="truA"
                     /locus_tag="ACFI8B_002134"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC3835244.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDITAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLIGEQDFS
                     SFRAAGCQAKSPVKTIYRTDLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
     gene            1972..3018
                     /gene="porB"
                     /locus_tag="ACFI8B_002135"
     CDS             1972..3018
                     /gene="porB"
                     /locus_tag="ACFI8B_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IB"
                     /protein_id="HFC3835245.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGAIKAGVQTYRSVEHTDGKV
                     SKVETGSEIADFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGTNTGWGNKQSFVGLKG
                     GFGTIRAGSLNSPLKNTKDNVNAWESGKFTGNVLEISGMAKREHRYLSVRYDSPEFAG
                     FSGSVQYAPKDNSGSNGESYHVGLNYQNSGFFAQYAGLFQRYGEGTKKIEYEHQVHSI
                     PSLFVEKLQVHRLVGGYDNNALYVSVAAQQQDAKLYGASRANSHNSQTEVAATAAYRF
                     GNVTPRVSYAHGFKGTVDSADHDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADK
                     IVSTASAVVLRHKF"
BASE COUNT          765 a          930 c          747 g          621 t
ORIGIN      
        1 ggtgcttcat cactttaggg aaccgttccc tttgagccgg ggcggggcaa cgccgtaccg
       61 gtttttgtta atccgctata cctgcgtaat ggtatcgggt actcatcatc aacctgcctt
      121 gcattcggtt gtgttgtccg gcaactgtcc ggttgtgccg atgggttgcc ctgccgctcc
      181 ctgagccgcg caacggttgt ctgccttggg cgggcgcggg tggcggccgg gcggtttgtc
      241 gctatgatac ggaagttccg atatacaagg gcgggcttca gcccgccaaa tccaccaact
      301 ccgccgaatc caccgattcc cgccgccgtc attcccgcat aagcgggaat ccagacccgt
      361 cggcacggga acctgcatcc cgtcattccc acaaaagtgg gaatccggaa cgaaaaatct
      421 caagaaaccg ttttacccga taagtttccg cactgacaga cctagattcc cgcctgcgcg
      481 ggaatgacgg ttcgggcact cctgacaggg tgaattcaca ggatagcgat tcgtagcaac
      541 tgcatccaac cccccgaaca actccccaaa caacgccgcg cgccccgggc gtttgccgtt
      601 tccctgcaaa atctgcgata caatgcagtc tgaacattta tccgaatccc aaacccgatg
      661 gataccgcac aaaaacaacg ctgggcaata accctatcct acgacggcag ccgcttttac
      721 ggctggcaga aacaggcggg cggcgtaccg accgttcagg cggcattgga aaccgcactc
      781 gcccgaatcg caggagaatc tgtcgccacc acagttgccg gcaggaccga caccggcgtg
      841 cacgctgccg cccaagtcgt ccactttgac ataactgccg cccgtcccca acaggcatgg
      901 gtgcgcggcg taaatgccca cctgcccgaa ggcattgccg ttttgcacgc ccgacaggtc
      961 gcacccggat ttcacgcccg cttcgacgca tacggacggc actaccgcta cctgctcgaa
     1021 tccgcccccg tccgttcccc cctgctcaaa aacagggcag gctggacaca cctcaaactc
     1081 gacatcgaac cgatgcgccg ggctgccgcc ttattgatcg gcgaacaaga cttctccagc
     1141 ttccgcgccg ccgggtgcca ggcaaaatcc cccgtcaaaa ccatctaccg caccgacctt
     1201 acccaaagcg caggactgat ccgcctcgat ttgcacggca acgccttttt gcaccatatg
     1261 gtacgcaaca tcatgggcgc gctcgtttat gtcggcagcg gcaggctcag cgtcgaaggc
     1321 ttcgccgcac tgattcaaga acgcagccgc ctcaaagccc caccgacctt catgcccgac
     1381 gggctttacc tgaccggcgt cgactatccc ggggcatacg gcatcgtccg cccccaaatc
     1441 cccgaatggc tttaaaacat tcttgtcgcg gagattttga aatcggacaa actgtcaggc
     1501 aatctttttc aatgttgaca ctatagtgga ttaacaaaaa tcaggacaag gcggcgggcc
     1561 gcagacagta caaatggtac ggaaccggtt cgcccggtgc ttgggcgcct tagggaaccg
     1621 ttccctttgg gccggggcgg ggcaacgccg taccggtttt tgttaatccg ctataacccc
     1681 cggtttgcaa tgccctccaa taccctcccg agtaagtgtt gtaaaaatgc aaatcttaaa
     1741 aattttaaat aaccatatgt tacaaaacaa aaaatacccg taatatctct atccgtcctt
     1801 caaaatgcac atcggattcc acacaaaaac aggcagaagt ttgttttttc agacaggaac
     1861 atctatagtt tcagacatgt aaccgccgag cccctcggcg gtaaatgcaa agctaagcgg
     1921 cttggaaagc ccggcctgct taaatttctt aaccaaaaaa ggaatacagc aatgaaaaaa
     1981 tccctgattg ccctgacttt ggcagccctt cctgttgcgg caacggccga tgtcaccctg
     2041 tacggtgcca tcaaagccgg cgtacaaact taccgttctg tagaacatac agacggcaag
     2101 gtaagtaaag tggaaaccgg cagcgaaatc gccgacttcg gttcaaaaat cggcttcaaa
     2161 ggccaagaag acctcggcaa cggtctgaag gccgtttggc agttggaaca aggtgcctcc
     2221 gtcgccggca ctaacaccgg ctggggcaac aaacaatcct tcgtcggctt gaagggcggc
     2281 ttcggtacca tccgcgccgg tagcctgaac agccccctga aaaacaccaa ggacaacgtc
     2341 aatgcttggg aatccggcaa atttaccggc aatgtgctgg aaatcagcgg aatggccaaa
     2401 cgggaacacc gctacctgtc cgtacgctac gattctcccg aatttgccgg cttcagcggc
     2461 agcgtacaat acgcacctaa agacaattca ggctcaaacg gcgaatctta ccacgttggc
     2521 ttgaactacc aaaacagcgg cttcttcgca caatacgccg gcttgttcca aagatacggc
     2581 gaaggcacta aaaaaatcga atacgaacat caagttcata gtatccccag cctgtttgtt
     2641 gaaaaactgc aagttcaccg tttggtaggc ggttacgaca ataatgccct gtacgtttcc
     2701 gtagccgcgc aacaacaaga tgccaaattg tatggagcaa gtagggctaa ttcgcacaac
     2761 tctcaaaccg aagttgccgc taccgcggca taccgtttcg gcaatgtaac gccccgcgtt
     2821 tcttacgccc acggcttcaa aggcactgtt gatagtgcag accacgacaa tacttatgac
     2881 caagtggttg tcggtgcgga atacgacttc tccaaacgca cttctgcctt ggtttctgcc
     2941 ggctggttgc aagaaggcaa aggcgcagac aaaatcgtat cgactgccag cgccgtcgtt
     3001 ctgcgccaca aattctaatc tgcaaagatt ggtatcaaca aaaagcctgt cgccagacag
     3061 gct
//
LOCUS       DAXFWM010000118         3023 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000118 DAXFWM010000000
VERSION     DAXFWM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.118"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            6..81
                     /locus_tag="ACFI8B_002136"
     tRNA            6..81
                     /locus_tag="ACFI8B_002136"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="ACFI8B_002137"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="ACFI8B_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC3835246.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="ACFI8B_002138"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="ACFI8B_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC3835247.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="ACFI8B_002139"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="ACFI8B_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC3835248.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="ACFI8B_002140"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="ACFI8B_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC3835249.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2470..2970
                     /gene="rplJ"
                     /locus_tag="ACFI8B_002141"
     CDS             2470..2970
                     /gene="rplJ"
                     /locus_tag="ACFI8B_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC3835250.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARSLAALAEKK
                     AGEEAA"
BASE COUNT          833 a          535 c          756 g          899 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaatgttaa agtgaatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggagggttt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgccgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcga attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ttagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 tccccgtgtt tctggtttta ttggagggag ggctaataga cccacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaagga ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgag aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggc aaagccaatc catccccccc agttggtcct gctttgggtc
     1201 aacgtggttt gaatatcatg gaattctgta aagcatttaa tgctgcaacc caaggtatgg
     1261 aatctggttt gccaattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac tcctccggcc tctattctgt tgaaaaaagc cgccggtttg caaaaaggta
     1381 gttctaatcc tctgacaaac aaggtaggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtt cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggacg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagttcttc gctcttctgt tgaagctaac aaactgtacg caatcgacga
     1621 agcaattgcc ttggttaaaa aagctgctac cgctaaattt gacgaatctg ttgacgtatc
     1681 tttcaacttg ggcgttgatt ctcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggt actggtaaga caacccgtgt ggctgtattt actcaaggtg taaatgcaga
     1801 agctgctaaa gaggctggtg cggatgtcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgttgttat tgcttctcct gatgcaatgc gtatcgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaaa taggtacggt
     1981 tacccctaat gttgccgaag cggtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggacgc tatcgttaaa gccaaacctg ctgctgctaa
     2161 aggtcagtac ctgaaaaaag ttgctgtatc cagcactatg ggtttaggtg ttcgcgttga
     2221 tacatcaagc gtgaataact aatctaagaa attttcaagc aactctgttt ttgagttgct
     2281 tgaatttggg ctacttaaaa ttaagtagat gtccaagacc gtagggatcg taagatttaa
     2341 tcgtaactgc cctacgcaga cggtagtcct gaaacacatt gcaagattgc ttgtaagatg
     2401 tctttttagg ttaccgcgct ggtgggatat cgttttggta tcctgtttat aaacagtggg
     2461 aggtagacct tgagtctcaa tattgaaacc aagaaagtgg cggtcgagga aattagcgcg
     2521 gcaattgcta atgctcaaac cctcgtagtc gctgaatatc gcggtatcag tgtttccagt
     2581 atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt atttgcgcgt tctgaaaaat
     2641 actctggctc gtcgcgcagt gcaaggtact tcatttgtag aattggccga tcaaatggtt
     2701 ggtccgttgg tttacgctgc ttctgaagat gctgttgctg ctgctaaagt gttgcaccaa
     2761 ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt cttacaatgg cgaagtaatg
     2821 aatgctgctc aggttgctga gttggcttct attccgagcc gcgaagagct gttgtccaaa
     2881 ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc gcagtttggc tgctttggca
     2941 gagaaaaaag ccggcgaaga agctgcttaa tcgattttgt ttctgttaat caattatttt
     3001 ttaatacaat atttggagta aaa
//
LOCUS       DAXFWM010000119         2788 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000119 DAXFWM010000000
VERSION     DAXFWM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.119"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(146..484)
                     /locus_tag="ACFI8B_002142"
     CDS             complement(146..484)
                     /locus_tag="ACFI8B_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835251.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     DKGHFHLWKR"
     gene            complement(529..906)
                     /locus_tag="ACFI8B_002143"
     CDS             complement(529..906)
                     /locus_tag="ACFI8B_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835252.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            complement(964..1329)
                     /locus_tag="ACFI8B_002144"
     CDS             complement(964..1329)
                     /locus_tag="ACFI8B_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835253.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(1311..1850)
                     /locus_tag="ACFI8B_002145"
     CDS             complement(1311..1850)
                     /locus_tag="ACFI8B_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835254.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            complement(1893..2759)
                     /locus_tag="ACFI8B_002146"
     CDS             complement(1893..2759)
                     /locus_tag="ACFI8B_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC3835255.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRSELAAPVYIQTGSASFIPVPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGSG
                     SYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKAS
                     SGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAAND
                     RISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQS
                     SSELKLGVQHTF"
BASE COUNT          743 a          627 c          556 g          862 t
ORIGIN      
        1 tctatattct taaaaatttc atctttctta agataacaag acattctgac aatcataacc
       61 tgttcatcag atatattatt tatttgcatg gcgcgtataa cacgccatgc ctgattaaaa
      121 ttagtctccc ttaccttaga atttattatc ttttccaaag atgaaaatgc cccttatctt
      181 gaactctcca gttagaacct ggaagtttcg tacctcttaa atgtgtatta atatttctta
      241 tagtttcatt aaaatgccac gcgctgccga tttcaacggt aattttcgta ccgctttctt
      301 caaattccag gtattccccc attagcgaac gcaaagaagc agacgccatc ccggcttcgt
      361 tatgataaac ccgcctgccg ttgatataga cttccgcccc tgtccgtttc aaattccaaa
      421 aattccgtac tgaaatttcc atatcccgat attgtgcaga ccatgttttt tcgaaggttt
      481 tcataaaatt tcctatacct gtccaatcgg cacatatcaa ttgcattatt acatctcaat
      541 acgataaata tttcttaagt caaaatgcaa gcctgaccgt acctcaactg tcaaaatttt
      601 attatttttt attgatttta agacaatttc tgaaaaattc tcttcgcttt ctcccttttg
      661 tagaagcaca tcagaaaaaa taaaactttc ccgattaaat tcataaatat gtttcaacca
      721 tttacctcct ctttctgtaa ggcaagattc agtttcattc ttccttattg tataaatatt
      781 tccttcacaa aatctgaaat aaatccataa atccatctta tccataatta aagaaaaagt
      841 ttcacctcga gattttgtca acaattcgca aggttgcgat gttgcaatca aatagccgaa
      901 agacattttt tacctcatac atggtcgaaa tcagtttctg ttagttcaga atccattttt
      961 tcgtcaacaa ctgaatccgc atttttgaat taacgttttc atcagctgcc gtttatctaa
     1021 accggcaggt tcagtttcag aataagcctt atatgaagac tgtaagcatt tcagaaaaag
     1081 atcatcagaa ggcatatctg ccgaatcaaa tacaactgtt ttgattttgg tacttaccca
     1141 aaaccctttt tgctcttttt ctactatacg gaaattcaga atatttccaa ccgaatcaaa
     1201 agcacggtaa acatcatcca tcaaatcctg cggctctatt ttcttttcca attccgacaa
     1261 tccttgaaat atatccaaag acacatcttc aaatagaaaa aaaggaggag ttagaagcgg
     1321 tttttccatg atctgtccgt agattttgat tcccaagggc gatgacgacc aattccctgt
     1381 ccaggcaaag tcttgcccgt attatccgta actcgacgat gataatgggg aaattttcca
     1441 ataggatgac ctgttctatt accgaaaggg gctatccgca tattattgcc gattttaatc
     1501 tcacgtccat atttagcaaa ggaaacaacc tttcctgcag cacctacatc accaggaatt
     1561 gcgcctaatc cgccagcaac atctctaaca gaagctggtc tgcctgtcgt tgcataacta
     1621 aaaccatgct gtgtccacat accaatggca gcaccaccca agatagccaa tggaagaaaa
     1681 gccccttcag tctccttcat ctccttctga gaaagctccg ccaactgcat cggcgcatct
     1741 gcccgcgtgt ggaacacttg gtcttcaaat gcctgattgt ccaatccgtt tgccattgcg
     1801 ggggcgatca tagacagcat cattacggct gcggtgattt gttttttcat aataactcct
     1861 ttggattaca aggttggaaa atcaaaacct gcttaaaatg tatgctgtac gcccaatttc
     1921 agttcggaac tgctttgccc tgaaacgttg aaacgtgcgg atgcgtttaa agccgcggtt
     1981 ttggtgaaac cgaaacctgc gccgaaatgg gcgtaggtgg atgtgtttct tgcggattct
     2041 tttttgccgt ctatgcggtc gggctgtttg cccagccatt ggatgcctcc ggtcaggctg
     2101 attctgtcgt tggcggcaaa tgagatgttg ggattcagca tccagtaatt gcctgctttg
     2161 tatttgacgt cgtctgaaag ggttttgctg ccgttgatgc ggtaggcggc ggtgagggaa
     2221 aggacaatcg gatctatggc tttgtaggtg gtggcgccga tgagccacga ttttcccgac
     2281 gaggctttgt tgcgcgattt ttcgtaaacc gtgctttcga ggaaagcgat gagtgcggga
     2341 tttttgccgt ctttgaggaa ggtgtggctg atgccggcgg atatgtcgga catccgtttg
     2401 ttgcgggttt tgccgttgcc gtcgagtttg cgttcttcgt gccacagata gctgccgctg
     2461 ccgtaaatgt cggtattgcc ggtcagtccg tagcgcaaac cgagcgtgcc ggcgagcata
     2521 tcggtattgc tgccgttttc ctgaatttcg gtcgggacgg ggataaagga agcggagccg
     2581 gtctgaatgt aaaccggtgc ggcaagttcg gaacggctgt tttcgctatt caggtaggta
     2641 agggaagttt ccagtttcca ttttcccttg tcggtcatta tgtcttcgat attcagcggc
     2701 agatcagtgc ggacacataa tggcacaaag ctaacaaata ctaagagaat atttttcatg
     2761 acattgtttt cttgattgaa atcaaagt
//
LOCUS       DAXFWM010000120         2787 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000120 DAXFWM010000000
VERSION     DAXFWM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.120"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            187..786
                     /locus_tag="ACFI8B_002147"
     CDS             187..786
                     /locus_tag="ACFI8B_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC3835256.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGYKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQK
                     KAIIQENMNRNGGVVKSDQSGEVLVRPKKSQKGITPPIK"
     gene            803..892
                     /locus_tag="ACFI8B_002148"
     CDS             803..892
                     /locus_tag="ACFI8B_002148"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835257.1"
                     /translation="MQAKSKGGSNSYKNAQVLSRRENIKKSNK"
     gene            901..1173
                     /locus_tag="ACFI8B_002149"
     CDS             901..1173
                     /locus_tag="ACFI8B_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835258.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            1340..1570
                     /locus_tag="ACFI8B_002150"
     CDS             1340..1570
                     /locus_tag="ACFI8B_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC3835259.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1719..2348
                     /locus_tag="ACFI8B_002151"
     CDS             1719..2348
                     /locus_tag="ACFI8B_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC3835260.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGRTQTVRNTVV
                     KPKPLKAYNLTAADWHTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESKIAELTNKIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            2329..2784
                     /locus_tag="ACFI8B_002152"
     CDS             2329..2784
                     /locus_tag="ACFI8B_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835261.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEIFKQLFHYPKLNDID
                     KCYLSNESYPTYIYDFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT          969 a          554 c          558 g          706 t
ORIGIN      
        1 ccaaacctaa aaactgtctg aaactcaatt tggttttcag taagcgtagg ttggcttaaa
       61 aacccaacca ccaaaatgcc gtctgaagcg gtattcagct ttcagacggc attttgatga
      121 atgaaacagg atattgagaa ctaagttctt taaaaatcct acacctgccc tttccacggc
      181 agcaccttgg tcaaaacggc agacggctac aaagccattg cccgtatccg agccggcgag
      241 agcgtcctct ccaaggacga ggcaagcggg gtaacgggat acaaacccgt taccgcccga
      301 tacggtaatc cgtatcggga aaccgtttac attgaagttt cagacggcat cggcaacagc
      361 caaaccctga tttccaaccg catccactcg ttttattcgg gcggcaaatg gattaaggcg
      421 gaagatttaa aagcgggaag cagactgtta tccgaaagcg gcagaaccca aaccgtccgc
      481 aaaaccgttg tcaaaccaaa accgctcaaa gcctacaatc tgaccgttgc cgactggcat
      541 acctacttcg tcaagggcag tcaggcggaa acggaagggg tttgggttca taatgattgt
      601 ccgtatggaa atttatcgga taataaatct gttggcgaag gaaaaaaatt tacaccagct
      661 caaaagaaag caatcatcca agaaaacatg aatagaaacg gaggggtagt gaagtctgac
      721 caatctggag aagttttggt tcgtcctaaa aagagccaaa aaggtatcac cccccccatc
      781 aaatgaatgg cagattgacc atgtgcaagc aaaaagtaaa ggaggttcaa atagttataa
      841 aaatgcacag gttttatcca gaagagaaaa tattaagaaa tcaaataaat aggtatcaat
      901 atgcaatgga aatttgaaga tcctcaaaat actgctgtta ttaccaccag aagaattttg
      961 tctaaaaaaa acggaatctt agaagtatgg catgatgctg atgatggaat gtggcaattc
     1021 ttagatggca atgaaattca tgaaggagat gctgtgatag ttagtttaaa agaaattgtc
     1081 actattgatt cctcagtcaa tctcttaagt aatttacctc ttggtggtta cgcttggaga
     1141 aatactatat cggaaaaatg gaattttgat tagcaagcgt aggttaggtt aaaaaaacca
     1201 acaatcaaag gtcgtctgaa acagtgttta gtttttcaaa cgaccttttc ttcaattaac
     1261 atagaatgtt gggtctcgac ccaacctaca cgcttactaa tctcaaatat aaccttatga
     1321 aataatgagt atttaaaaga tggaaacatt agacgaacga ataaagaatt taggaaaatc
     1381 tcttgaagat agaattgacg ctaatctaat tgatgccaca ctagagtaca ttactttttc
     1441 agaacgtttg ctagcttttg aaacgctatg tgattatatt gaagatttca atatccagct
     1501 tacggaaaaa gaatcccaag aaatctcttt tattaataag gaatttggaa ttgaaagtac
     1561 atcagattaa taaatattag gtcggtttaa aaacccaaca atcacaaggt cttctgaaac
     1621 cgtgtttaat tttcagacgg catttccttc atttgaaaca ggatattgaa aactaagttc
     1681 ttcaaaaatc ctacacctgc tctttccacg gcagcacctt ggtcagaacg gcagacggct
     1741 acaaagccat tgcccatatt caagccggag accgcgtcct ttccaaggac gaggcaagcg
     1801 gggagacggg atacaaaccc gttaccgccc gatacggtaa tccgtaccaa gaaaccgttt
     1861 acattgaagt ttcagacggc atcggcaaca gccaaaccct gatttccaac cgcatccatc
     1921 cgttttattc gggcggcaaa tggattaaag cggaagattt aaaggcggga agcagactgt
     1981 tatccgaaag cggcagaacc caaaccgtcc gcaacaccgt tgtcaaacca aaaccgctca
     2041 aagcctacaa tctgaccgct gccgactggc atacttactt cgtcaagggt aatcaggcgg
     2101 aaacggaagg ggtttgggtt cataattctt gtccgcctaa aagaacagga agctccaaga
     2161 atgaaaaaca tggagacggc ggtcgaagtc aaatatcagc ggaatcaaaa attgctgaat
     2221 taacaaataa aattattccc ggaatgtcca aaaatgaacg cttaaagatt aagcaaaaaa
     2281 tcaaaaatat tgccaaaaat gccaatcgaa aaacaaaagg agaagagcat ggtcgacgcg
     2341 gtcgttaaaa ctcccgaatt tttacctttt acttctgtag gaatattccg ttttggtgct
     2401 gatataacac agtacaaaaa catactggaa acttttatgt atgaacctcc tgatgagttt
     2461 ggaacagaat attatgaatc tcccgactca aatttactca tttccgtcaa aaaaaacaaa
     2521 attatttcga tattttgtta tcaggagcta tattttatgg gcgttaatat aataggtcta
     2581 aattttgaaa tttttaaaca acttttccat taccccaagt taaacgatat tgataaatgt
     2641 tatttatcga atgaatctta tccgacatat atctatgatt ttgatgaaat aggagtacaa
     2701 gcatgggaag caaaagggaa aattgttact atcattgccg ggggcaaaga caattattcg
     2761 acagaacctt actacgatga ataacat
//
LOCUS       DAXFWM010000121         2692 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000121 DAXFWM010000000
VERSION     DAXFWM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.121"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            101..379
                     /locus_tag="ACFI8B_002153"
     CDS             101..379
                     /locus_tag="ACFI8B_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HFC3835262.1"
                     /translation="MKTRNIAFKFAVLAAVLAAGYAFGFAKGGGSRTAKGKPADIAAM
                     RMALAQKQAEVDELSAEIWLEERHLNADEEEAGCRRVHGDAEVPEGKE"
     gene            376..537
                     /locus_tag="ACFI8B_002154"
     CDS             376..537
                     /locus_tag="ACFI8B_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835263.1"
                     /translation="MSFHPETAYNGGGETEPYGPSPEEIKYRQSPETAETRRMTEKQA
                     EGHIKSIIR"
     gene            606..1292
                     /locus_tag="ACFI8B_002155"
     CDS             606..1292
                     /locus_tag="ACFI8B_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HFC3835264.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDNAVFAFDLS
                     DPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQAAEGRTEHPQGNAAPA
                     EDIEDDIPFN"
     gene            1432..1614
                     /locus_tag="ACFI8B_002156"
     CDS             1432..1614
                     /locus_tag="ACFI8B_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835265.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            1611..2102
                     /locus_tag="ACFI8B_002157"
     CDS             1611..2102
                     /locus_tag="ACFI8B_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC3835266.1"
                     /translation="MTALALYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEGHIRRMTGKLKAVKAQNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEGRKNL
                     QIR"
     gene            2154..2369
                     /locus_tag="ACFI8B_002158"
     CDS             2154..2369
                     /locus_tag="ACFI8B_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835267.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            2480..>2692
                     /locus_tag="ACFI8B_002159"
     CDS             2480..>2692
                     /locus_tag="ACFI8B_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835268.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVRNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          847 a          678 c          729 g          438 t
ORIGIN      
        1 ttgtagattt tagccgcaga taaccccgac ggcgcacggt ccgtaccatc cttagcggac
       61 gcggtgcgca attaaaacct tttaaattaa ggaaatagaa atgaagacaa ggaatattgc
      121 gtttaagttc gcggtattgg cggcggtatt ggcggcgggc tatgccttcg gattcgccaa
      181 gggaggcgga agccgtacgg caaagggaaa acccgccgat attgcggcaa tgcgcatggc
      241 gttggctcaa aagcaggcgg aagttgacga attgagcgcc gagatctggc tggaagagcg
      301 gcatctgaat gccgatgaag aagaagccgg ttgccggcgg gtgcacggcg atgcggaagt
      361 gccggagggt aaagaatgag cttccatccc gaaaccgctt ataacggcgg cggagaaaca
      421 gagccgtacg gaccaagccc cgaagaaatc aaataccggc aaagcccgga aaccgccgaa
      481 acacggcgga tgaccgaaaa acaggcagaa ggccacatta aaagcattat caggtaacgc
      541 cgccgcgcaa ggcataaaac agcgcgagac cgatagacga aacgaccttt aacaggagac
      601 cgaaaatgtc attgatttta agtgtgaaag acgaaagcaa tttcaaacca tgcccggcag
      661 gcagccatca cgccacctgc atccgcatca tcgatttggg tacgcagctc gtcgagtacc
      721 aaaacgaaca aaagcggcag cacaagattt tagtgcagtg ggaaatcgac cctgaaggcg
      781 atccggaaat gctgatgccg gacggcaggc cctacctcat cagccgccga tacaccgcca
      841 gcctgcacag caaaagccaa ctggcaacag acctcaaaag ctggcgcgga agggacttta
      901 caccggaaga acgcgacaac ttcgatttgc gcaatatttt gggcaagccc tgcctgttga
      961 gtatcgccca ccaagaaagc agcgacggca aaaccaccta tgccaacatt tccgccatca
     1021 gcaacaagat gaagagctac accccgaaac agccggacaa cgccgttttt gccttcgacc
     1081 tgtcagaccc cgattgggcg aattacggcc tcctgaacga gaaactgagg gagcagattg
     1141 ccaaaagccc ggaatatgcc gaagccgtaa acggccgcca accgccggcg ccgccgcaga
     1201 aacaggccca agcggcggaa gggcggacgg aacaccccca aggcaatgcc gcgcctgccg
     1261 aagacatcga ggacgacatc ccttttaatt aagcccgccg tcagggcgta aaaccggacg
     1321 gaaaaccgca aacaaacgcc gcaccctgcg ggcaggcccc gcactctgaa gcagtagtcg
     1381 atttttagtt tgcaactgct tcggacggca acagagaaag gaaacaaaaa aatggcaaac
     1441 atcgacctga cccaatggga cgggaaaacc attggcgccg ccgccaatcc cgaacagggc
     1501 tacatcaaca tcaccatcgg cagcgacgac ctattcatca acatcgaaca ggcatacgcc
     1561 atacacgccg cgcttggcga agcggttgcc gaatatgagg gaggggcaca atgaccgccc
     1621 tcgcactcta ccggtgcgcg gcagacgtac aggcggcgct tgattactac tttgacagcg
     1681 aaaccgagcg cgaagacacg ctggaagccg ttatcgggca gttcgaggtc aaagcgcaat
     1741 ccgttatcgc ttatattaaa aaccaagaaa tcacggaaaa aatgcttgaa gggcacatca
     1801 ggcggatgac cgggaagctc aaggcggtaa aagcgcaaaa tcaaagcctg aaagactact
     1861 tggcgcgcaa tatgcaggcg gcgggcatta ccgaaatcaa agcggacgac ggcactttta
     1921 aagcctcgtt ccgcaaatcc gaagccgtcg tgatcttaga cgaagcacaa atccccgccg
     1981 aatttatgcg tgaggccgtc aaaaccgaac cggacaaaac cgccatcaga aaagcgattg
     2041 aaagcggtcg gcaagtagca ggcgcgaaga ttgaagggcg gaagaatttg cagattagat
     2101 aaaccgtaga agatgttgac gacagcatcc cattttgagt taaggagcaa aaaatgagct
     2161 atttggaaga tgtaaaaaac gcattaaggg taatagataa cttatgcaaa gaagcattaa
     2221 aagagcctga atcgttagag ggttatatag acgaaattag ggataaagca gacgaagcgg
     2281 atacctcttt ggaatttcta aaggatgtaa taaattatgg tattagcgat ttaaaaaatg
     2341 taattgaggt gtttgaagat tgcgtttgat attaatggaa caagacatga actatcaatt
     2401 taaattcggc gacctcgtga gagatacagc tttatgattg ggcattgaga gaacggcatc
     2461 aaaaaatcga gaaaacaaaa tgcaaacagt agcaacaaaa ccgacggcaa aacagatgct
     2521 tgccgccaaa cgcgcggcga aggaatcaac gcggcaggaa cgcgccgtca aacgcgccgg
     2581 gacggtcaga aacgttgacc ggaaccggct gtccgcccgg tcaaaagcgc aaaaagaaaa
     2641 catcgcccgg atgttgtcgg gcgcaaaggt atcggaagac gaagccctga cg
//
LOCUS       DAXFWM010000122         2565 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000122 DAXFWM010000000
VERSION     DAXFWM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.122"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..504
                     /locus_tag="ACFI8B_002160"
     CDS             <1..504
                     /locus_tag="ACFI8B_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3835269.1"
                     /translation="KDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGY
                     IISRKAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKF
                     HDQNSALGSLIEHDRRLNRKQQRRDSPANTFKHRLIRALTKIGREREKRRKRREQTIG
                     KIIVPFQ"
     gene            516..1436
                     /locus_tag="ACFI8B_002161"
     CDS             516..1436
                     /locus_tag="ACFI8B_002161"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFC3835270.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAAVAANLRGGGNIRFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     TDVLVRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     GFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEE
                     WRGKLAVPPTKHMLQRWRKKLSARFLRKIY"
     gene            1496..2509
                     /locus_tag="ACFI8B_002162"
     CDS             1496..2509
                     /locus_tag="ACFI8B_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3835271.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDILIVDDGSTD
                     GTPAIARHFQEQDGRIRIISNPRNLGFIASLNIGLDELAKSGGGEYIARTDADDIAAP
                     GWIEKIVGEMEKDRSIIAMGAWLEVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVF
                     PFGNPIHNNTMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRF
                     HQDQTSSKYNLQQRRTAWKIKEEIRAGYWKAAGIAVGADCLNYGLLKSTAYALYEKAL
                     SGQDIGCLRLFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKY
                     RSY"
BASE COUNT          647 a          651 c          703 g          564 t
ORIGIN      
        1 aaggattctg cctttatcgt ccgtttggaa acgatgttta tgcacgtcct gacctcgccc
       61 tccggcgtgg cggactacgg cgggcgcgcc tttccgcttt tggaaagcga acattgtggg
      121 acggctggct atattatttc ccgaaaggcg atgcgttttt tcttggacag gtttgccgtt
      181 ttgccgcccg aacgcctgca ccctgtcgat ttgatgatgt tcggcaaccc tgacgacagg
      241 gaaggaatgc cggtttgcca gctcaatccc gccttgtgcg cccaagagct gcattatgcc
      301 aagtttcacg accaaaacag cgcattgggc agcctgatcg aacatgaccg ccgcctgaac
      361 cgcaaacagc aaaggcgcga ttcccccgcc aacacattca aacaccgcct gatccgcgcc
      421 ttgaccaaaa tcggcaggga aagggaaaaa cgccggaaaa ggcgcgaaca gacaatcggc
      481 aagattattg tgcctttcca ataaaaggag aaaagatgga catcgtattt gcggcagacg
      541 acaactatgc cgcctacctt tgcgttgcgg caaaaagcgt ggaagcggcc catcccgata
      601 cggaaatcag gttccacgtc ctcgatgccg gcatcagtga ggaaaaccgg gcggcggttg
      661 ccgccaattt gcgggggggg ggtaatatcc gctttataga cgtaaacccc gaagatttcg
      721 ccggcttccc cttaaacatc aggcacattt ccattacgac ttatgcccgc ctgaaattgg
      781 gcgaatacat tgccgattgc gacaaagtcc tgtatctgga tacggacgta ttggtcaggg
      841 acggcctgaa gcccttatgg gataccgatt tgggcggtaa ctgggtcggc gcgtgcatcg
      901 atttgtttgt cgaaaggcag gaaggataca aacaaaaaat cggtatggcg gacggagaat
      961 attatttcaa tgccggcgta ttgctgatca acctgaaaaa gtggcggcgg cacgatattt
     1021 tcaaaatgtc ctgcgaatgg gtggaacaat acaaggacgt gatgcaatat caggatcagg
     1081 acattttgaa cgggctgttt aaaggcgggg tgtgttatgc gaacagccgt ttcaacttta
     1141 tgccgaccaa ttatgccttt atggcgaacg ggtttgcgtc ccgccatacc gacccgcttt
     1201 acctcgaccg taccaatacg gcgatgcccg tcgccgtcag ccattattgc ggctcggcaa
     1261 agccgtggca cagggactgc accgtttggg gtgcggaacg tttcacagag ttggccggca
     1321 gcctgacgac cgttcccgaa gaatggcgcg gcaaacttgc cgtcccgccg acaaagcata
     1381 tgcttcaaag atggcgcaaa aagctgtctg ccagattctt acgcaagatt tattgacggg
     1441 gcaggccgtc tgaagccttc agacggcatc ggacgtatcg gaaaggagaa acggattgca
     1501 gcctttagtc agcgtattga tttgcgccta caacgtagaa aaatattttg cccaatcgct
     1561 gtccgccgta gtggggcaga cttggcgcaa cttggatatt ttgattgtcg atgacggctc
     1621 gacggacggc acgcccgcca ttgcccggca tttccaagaa caggacggca ggatcaggat
     1681 aatttccaat ccccgcaatt tgggctttat cgcctcttta aacatcgggc tggacgaatt
     1741 ggcaaagtcg gggggggggg aatatattgc gcgcaccgat gccgacgata ttgccgcccc
     1801 cggctggatt gagaaaatcg tgggcgagat ggaaaaagac cgcagcatca ttgcgatggg
     1861 cgcgtggttg gaagttttgt cggaagaaaa caataaaagc gtgcttgccg ccattgcccg
     1921 aaacggcgca atttgggaca aaccgacccg gcatgaagac attgtcgccg ttttcccttt
     1981 cggcaacccc atacacaaca acacgatgat tatgaggcgc agcgtcattg acggcggttt
     2041 gcggttcgat ccagcctata tccacgccga agactataag ttttggtacg aagccggcaa
     2101 actgggcagg ctggcttatt atcccgaagc cttggtcaaa taccgcttcc atcaagacca
     2161 gacttcttcc aaatacaacc tgcaacagcg caggacggcg tggaaaatca aagaagaaat
     2221 cagggcgggg tattggaagg cggcaggcat agccgtcggg gcggactgcc tgaattacgg
     2281 gcttttgaaa tcaacggcat atgcgttgta cgaaaaagcc ttgtccggac aggatatcgg
     2341 atgcctccgc ctgttcctgt acgaatattt cttgtcgttg gaaaagtatt ctttgaccga
     2401 tttgctggat ttcttgacag accgcgtgat gaggaagctg tttgccgcac cgcaatatag
     2461 gaaaatcctg aaaaaaatgt tacgcccttg gaaataccgc agctattgaa accgaacagg
     2521 ataaatcatg caaaaccacg ttatcagctt ggcttccgcc gcaga
//
LOCUS       DAXFWM010000123         2455 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000123 DAXFWM010000000
VERSION     DAXFWM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.123"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            460..1383
                     /gene="lpxC"
                     /locus_tag="ACFI8B_002163"
     CDS             460..1383
                     /gene="lpxC"
                     /locus_tag="ACFI8B_002163"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC3835272.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
     gene            1511..1924
                     /locus_tag="ACFI8B_002164"
                     /pseudo
     CDS             1511..1924
                     /locus_tag="ACFI8B_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          623 a          669 c          606 g          557 t
ORIGIN      
        1 gcgcgggaat ctaggccggt cggtgcggaa acttatcggg aaaaaaggtt tcttgagatt
       61 ttacgtcctg gattcccact tttgtgggaa tgacgtggtg caggtttccg tacggatgga
      121 ttcgtcattc ccgcgcaggc gggaatccgg accttagaac aacagcaata ttcaaagatt
      181 atctgaaagt ccgggattct agattcccgc ccgcgcggga atgacgggat gtatagtgaa
      241 ttaaatttaa accagtacgg cgttgcctcg ccttttcgta ctatccgtac tgcctgcggc
      301 ccgccgcctt gtcctgattt tcgttaatcc gccatacatt ttcatgcggc ttacgtgttg
      361 gaaacggata cattggcaaa tactattgaa aaacatagcc tgtttttccg gttttatcgt
      421 attattccga aacttacccc attgataagg aagcggttta tgctgcaaag aactttggcg
      481 aaatccatcg gcgttaccgg agtcgggctg cattcgggcg aacgcgtcgc gctgaccctg
      541 caccctgcgc ctgaaaacag cgggatttcc ttccgccgca ccgatttgga cggcgagatg
      601 ggcgaacaaa tcaagctgaa cccttatttg atcaacgata cccgcctttc atccaccatc
      661 gttaccgaca aaggcctgcg cgtcggcacc atcgaacaca tcatgtccgc actgtccgcc
      721 tacggcatcg acaatgcgct gattgagttg aacgcgcccg aaatcccgat tatggacggc
      781 tccagcctgc cgtttattta cctcctgcaa gatgcgggcg ttgtagacca aaaggcgcaa
      841 aagcgttttt tgaaaatcct caaacccgtc gaaatcaaag aagcgggcaa atgggttcga
      901 tttacgccgt atgacggttt taaggtaacg ctgaccatcg aattcgacca tcctgttttc
      961 aaccgcagcc cgcctacgtt tgaaattgat ttcgccggca aatcctacat cggcgaaatc
     1021 gcgcgcgcgc gcactttcgg ctttatgcac gaagtggaaa tgatgcgcgc ccacaatctc
     1081 ggcttaggcg gcaatttgaa caacgccatc gtgattggcg acacggatgt cctgaatcct
     1141 gaaggcttgc gctatcccga tgagtttgtg cgccacaaaa tcctcgacgc catcggcgat
     1201 ttgtatatcg tcggacatcc gattgtcggc gcgtttgaag gctacaaatc gggacacgcc
     1261 gtcaacaacg cccttttgcg cgcagttttg gcagacgaaa cggcttacga atgggtggaa
     1321 tttgccgaca gcgatgattt gcccgacgca tttcacgagc tgaacatcag aaattgcgga
     1381 taacgccccc ttttccaaac aaatgccgtc aaacagatgc cgtcattccc gcgcgggcgg
     1441 gaatctagga cgtaaaatct aaagaaaccg ttttcccgat aagtttccgt gccgacagac
     1501 ccggattccc gcccgcgcgc aagtttccga agccatcctt ttggccgaag gtcaaaaatc
     1561 agccgttacc gagtattacc tgaataacgg cgaatggccg aaagacaaca cttctgccgg
     1621 cgtggcatcc gcttcaacaa tcaaaggcaa atatgttaaa agcgttacgg tcacaaacgg
     1681 cgtcgttacc gcccaaatga atccaagcgg cgtaaacaat gaaatcaaag gcaaaaaact
     1741 ctccctgtgg gccaagcgtc aagacggttc ggtaaaatgg ttctgcggac agccggttaa
     1801 gcgcaccgaa gccaacgcca aagccggcac cgacgacgtc gccaaagacg acaccgccgg
     1861 caccaaaatc gacaccaagc acctgccgtc aacctgccgc gatacgtcat ctgccggtac
     1921 gtgatccacg gccgcccgga tcaacccggg cggcttgtct tttaaaggtt tgcaaggcgg
     1981 gcggggtcgt ccgtttcggt ggaaataata tacccgcgcg caagtttccg aagccatcct
     2041 tttggccgaa ggtcaaaaaa aggtcaaaaa tcagccgttg ccgggtattg cccgaatcac
     2101 ggcatatggc cggaaaactt cgtcattccc gcgaaagcgg gaatccagtc cgttcagttt
     2161 cggtcatttc cgataaattc ctgttgcttt tcatttctag attcccgcct gcgcgggaat
     2221 gacggcggag cggtttccgt tgctcccgat aaattcctaa aacttaaaat ttcatcattc
     2281 ctacaaggac agaaaaccaa aaacagaaac ctaaaattcg tcattcccgc aaaagcggga
     2341 atctagcctt gtcggcacgg aaacttatcg ggtaaaaagg tttctccggt cctgagtcct
     2401 ggattcccac tttcgtggga atgacgggat ttaatgatgc cgccggcaac gaaaa
//
LOCUS       DAXFWM010000124         2273 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000124 DAXFWM010000000
VERSION     DAXFWM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.124"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(114..1082)
                     /gene="ispH"
                     /locus_tag="ACFI8B_002165"
                     /gene_synonym="lytB"
     CDS             complement(114..1082)
                     /gene="ispH"
                     /locus_tag="ACFI8B_002165"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC3835273.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1112..1606)
                     /gene="lspA"
                     /locus_tag="ACFI8B_002166"
     CDS             complement(1112..1606)
                     /gene="lspA"
                     /locus_tag="ACFI8B_002166"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC3835274.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          516 a          652 c          579 g          526 t
ORIGIN      
        1 tcatgggaaa tcccctaaat gccttggtgg gaatttaggg gattttgggg atttttgcaa
       61 aggtctcggc atttgtattg cccaaacatc cgatgcctgc gttaacgcct gcgtcaacgt
      121 ttgcacaaat cgggtttggt ttcgccctcg cggcgcagct ccttgggcag gacgaacacg
      181 atgctttctt ccgcgccccc cccttcgcgc acggtttcat gcccccatcc gcgtatggct
      241 gccaatactt cccgcaccaa cacttcgggc gcggacgcgc ctgccgttac gccgactttg
      301 cttttgcctt caaaccacgt gcgttgcagg taggacgcgt tgtccaccat atacgcatcg
      361 attccgcgcg atgccgccac ttcgcgcagg cggttgctgt tggacgaatt gggcgaaccg
      421 accacaatca cgatgtcgca ctgttccgcc agctctttga cggcggtttg ccggttggtc
      481 gtcgcatagc agatgtcttc cttgtgcgga ttgcggatat tggggaaacg cgcgttcagc
      541 gcggcgatga tgtctttggt ttcatcgacc gagagcgtgg tttggctgac ataggcgagt
      601 ttgtcggggt ttctgacttc gagttttgcc acatctccga ccgtttcgac caaaagcatt
      661 ttgcccggtg caagctgccc catcgtgcct tcgacctcgg cgtgcccctt atgcccgatc
      721 atgatgattt cacagttttg ggcatccagt cgggcgactt ccttatgcac tttcgttacc
      781 agcgggcaag tcgcatcaaa tacccggaaa ccgcgctccg ccgcttcctg ctgcaccgcc
      841 ttcgatacgc cgtgtgccga ataaaccagt gtcgcgcccg gcggcacttc cgccaagtct
      901 tcgataaaca ccgcgccttt ttcgcgcagg ttgtccacga cgaatttgtt gtggacgact
      961 tcgtggcgca cataaaccgg cgcgccgaat tcttccaaag cacgttcgac aatactgatt
     1021 gcccgatcca caccggcgca gaagccgcgc ggattggcaa ggatgatggt ttttccgttc
     1081 ataagttttg cattccgtgt tcagacggca ttcacgtttt tttgctatct ttgcgatgga
     1141 cgatattgtc aagcaccgcc aacaccgcac cgacgcagat aaagctgtcg gcaatattaa
     1201 aggcgggata aaaccaattt tgccaataaa acaataagaa atcgacgaca tgaccgtgta
     1261 tcaggcgatc gatgacattg cccgacgcac cgccgataat cattgccgcc ccgattttgc
     1321 cgagggctgc aaactcgtcg cgcaagatgg cgcgtaccaa atacgcgctc accgccaccg
     1381 ccagcaccaa aaaaaagtat ttctgccagc cgccctgatc ggcaaggaag ctgaatgccg
     1441 cgcccgggtt gtacaccagc gtcagatcga aaaacgaagg gatgacgttg acgcgttccc
     1501 gatactgaaa cgacgacagc accgcccact tcgaccactg gtccagcacg atggcggcaa
     1561 gtgccaatac ccaatagcgc gttttacttg aaacagatga agacatattt ttcaacagcc
     1621 ggataaaaga gtgccattct acccgaaaac cccctttcct gtacccgaaa cggcaaatgc
     1681 cgtaatcttg aaacccgtca ttcccgcgca ggcgggaatc cggacctgtc cgcacggaaa
     1741 cttatcggat aaaacggttg cccaaacccc gcgtcctaga ttcccacttc cgtgggaatg
     1801 acggttcggt tgcgtagggc cgggtggtcg aaaaggcgga ttcgatggat tcgatgaaaa
     1861 cggtagacat gttggattga tggaaatggc gggccgaagc acaccctacg gcccgccgga
     1921 tttccgcctg cgcgggaatg acggggtttc aggttgctgt ttcaggttgc tgtttttaag
     1981 tttctgtttc ggtttgctat tttatagaaa tgacgaaatt ttagattgca agaacttatc
     2041 ccctccgccg tcattcccac gaaagtggga atctagaaat aaaaagcagc aggaatttat
     2101 cggaaataac tgaaaccgaa cggaccggat tcccgcctgc gcgggaatga cgaatccatc
     2161 cgtacggaaa cctgcaccac gtcattccca caaaagtggg aatccaggac gtaaaatctc
     2221 aagaaacctt ttttcccgat aagtttccgc accgaccggc ctagattccc gcc
//
LOCUS       DAXFWM010000125         2261 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000125 DAXFWM010000000
VERSION     DAXFWM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.125"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..104
                     /locus_tag="ACFI8B_002167"
     CDS             <1..104
                     /locus_tag="ACFI8B_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC3835275.1"
                     /translation="LEICNSTSEDKSISNLAKYFDWEFDDYNLNIQD"
     gene            181..390
                     /locus_tag="ACFI8B_002168"
     CDS             181..390
                     /locus_tag="ACFI8B_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835276.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
     gene            491..1339
                     /gene="mafB"
                     /locus_tag="ACFI8B_002169"
                     /pseudo
     CDS             491..1339
                     /gene="mafB"
                     /locus_tag="ACFI8B_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            1336..1692
                     /locus_tag="ACFI8B_002170"
     CDS             1336..1692
                     /locus_tag="ACFI8B_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC3835277.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILTVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            1866..2240
                     /locus_tag="ACFI8B_002171"
     CDS             1866..2240
                     /locus_tag="ACFI8B_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC3835278.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFKNSILPDLNLIPFAGNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
BASE COUNT          807 a          339 c          459 g          656 t
ORIGIN      
        1 tattggaaat atgtaattca acgtctgaag ataagtctat atcaaatttg gcaaaatatt
       61 ttgactggga atttgacgat tataatctaa atatccaaga ctaaagatgg tgggacagca
      121 tttagaccaa aaacaggtaa acagtatttt gatgacaaat taaaataaaa ggaattattc
      181 atgaaattaa ttgattttga agggaattta gttaaaatta gtctcgataa agatgagttg
      241 tatattattc aagcaattgt aggagaaatt tattcaggcg tatgcgtgga ttgtagagat
      301 tttgagataa ttcatggtgt tgaaaaaaat aaagttcttc ttttggataa agaacttaaa
      361 aaaatatatg atacttggga taaatgttag ctagataggg tgggcactcg ttgcccacct
      421 tttgccgtat attaaaaatc cttttcaggc tgcctgaaac tgggtgggca tcaatgccaa
      481 tcctaactgt gcggatgaag caggaaaact gatatgggaa aacgaccccg ataaaaattg
      541 gcgggctaac cgtatggacg atattcgcgg catcgtccaa ggtgcggtta atcctttttt
      601 aacgggtttt cagggattgg gagttggggc aattacagac agtgcggtaa acccggtaac
      661 ctatgcggca gcacggaaaa ctttacaggg tattcacaat ttaggaaatt taagtccgga
      721 agcacaactt gccgccgcga gcctattaca ggacagtgcc tttgcggtaa aagacggcat
      781 caattccgcc agacaatggg ctgatgccca tccgaatata acagcaacag cccaaactgc
      841 ccttgccgta gcagaggccg caggtacggt ttggggcggt aaaaaagtag aacttaaccc
      901 ggccaaatgg gattgggtta aaaataccgg ctatgaaaaa cctgctgccc gacctatgca
      961 gactgttgac ggggaaatgg caggtgggaa taaacctatt aaatctttac caaacagcgc
     1021 cgctgaaaaa agaaaacaaa gttttaagaa gtttagtagc aactggagtt cagcaagttt
     1081 tgattcagtg cacaaaacac taactcccaa tgcacctggt attttaagtc ctgataaagt
     1141 taaaactcga tacactagtt tagatggaaa aattacaatt ataaaagata acgaaaacaa
     1201 ctattttaga atccatgata attcacgaaa acagtatctt gattcaaatg gtaatgctgt
     1261 gaaaaccggt aatttacaag gtaagcaagc aaaagattat ttacaacaac aaactcatat
     1321 caggaactta gacaaatgaa tgaacacaac ctgttaattt tctgtttaaa agacaatgtt
     1381 tcaattagtg aatatactga aatggttgat tgggcttatg aaaacattca atctgaaaca
     1441 gttgtagaaa ttacggaaaa tcaaattatt gaatatcaaa atcgtggatt atggagactt
     1501 gtttctgaaa ttaccgataa ttggttattt ggaccaagtg agggggattg gctaatagat
     1561 aaggaaagta ttttgactgt aaaagaaaaa ttacaaaatt cagatttttc tacagagccc
     1621 ttagtgaaaa atattattca tgtacttgaa tatgctataa aaaatgaaaa aacagtaatt
     1681 tttcattttt gaaactaatc tagtttttag taatattgat gcagagcaag cagcattaga
     1741 tgccgcaaac atggggagaa gctattcaat ttagaattaa aaaaacaaag tgaaaatgga
     1801 ctagcaccac caaattggtc tacccagttt cctaatggta gtatttatga tactaagata
     1861 acgaaatgat tattcaaaat gaatttaatt tatatcctag taatatgctt cctgaagggt
     1921 tttgttatcc tgaaaagtat gttcgtatct ctaacgatac atctttaata ccttatattc
     1981 agccacataa ttttcactgg tggtttgaga attatggaac agaaggggca gaagtagctt
     2041 atatatttaa aaattccatc ctgcctgatt taaatcttat cccattcgct ggtaatggag
     2101 aatgggaagc ttattttgat ggtaatgatg taacaggaaa tcctagggtt attgttatta
     2161 atttagataa tatagaaaac catgaatttt ttaatagttt tgaagagtgg cttgaattag
     2221 caattaagga tacttggtaa gcagctatct ataaagagat g
//
LOCUS       DAXFWM010000126         2043 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000126 DAXFWM010000000
VERSION     DAXFWM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.126"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..272)
                     /locus_tag="ACFI8B_002172"
     CDS             complement(<1..272)
                     /locus_tag="ACFI8B_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3835279.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWS"
     gene            400..615
                     /locus_tag="ACFI8B_002173"
     CDS             400..615
                     /locus_tag="ACFI8B_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835280.1"
                     /translation="MTHSEFIKTLSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKR
                     LYKDVMLLTKNKVSISELISEFRVCPK"
     gene            695..850
                     /locus_tag="ACFI8B_002174"
     CDS             695..850
                     /locus_tag="ACFI8B_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835281.1"
                     /translation="MKKQDRNRLSKKDRRLIKKAMLKAAAKGSDEVYRIAPGLKDGFE
                     LLGKQPD"
     gene            complement(827..1015)
                     /locus_tag="ACFI8B_002175"
     CDS             complement(827..1015)
                     /locus_tag="ACFI8B_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835282.1"
                     /translation="MPSDLSFILAKELIRSGSIRLSGSTAKGQAGELAVFIRTLHQKP
                     EESEPNTDDEYLIGLLSK"
     gene            1189..1416
                     /locus_tag="ACFI8B_002176"
     CDS             1189..1416
                     /locus_tag="ACFI8B_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3835283.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPRMKAGGRGKPVTHYSIVKNGAEV"
     gene            1413..>2043
                     /locus_tag="ACFI8B_002177"
     CDS             1413..>2043
                     /locus_tag="ACFI8B_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3835284.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDEGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEPLDYEPSDYEPSDFDAKNHQILSGEPSDFALRTIGFCAKNHQILSDEPSD
                     FDGSLYVEPSVEPSVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPE"
BASE COUNT          562 a          457 c          543 g          481 t
ORIGIN      
        1 ctgctccaca tcatcgggcg gtggatgtct ttgacttcgt gcggcaggtc ggggttgatg
       61 aggacgggcg ttggttcgct cggaacggaa tccaagtaca gactgcccat cccatattct
      121 tcttcaagcc gtctgcttac cttttcgcca aaagatgctt taccgctcat caattgagat
      181 aaaaggcttt tgtctttaac cggcaacttt ttattttcaa agaatctttt taaattatcg
      241 atacggatgg ccttcagatc tctattagtc attttcgcgc tccattcaga ttgggattca
      301 attttattta gtttctgcta aacaaacaaa tattaaattt tcttgcctat gcgtttaata
      361 tttgctaaac tcattaaata tttacggtga aactacagaa tgactcattc agaatttatt
      421 aaaacgctgt cagaacagcg tggtgcaaaa acggaatacg ctaaaaaact tggcttatcc
      481 ctgtcttttc tatggcagat agaaaacgga cgggcagtaa cgccaaagcg tctttataaa
      541 gacgttatgt tgttgacaaa aaataaggtt tctatttctg aattaatttc ggagttcagg
      601 gtttgtccca aataaaaagc ccgtcgggtc agatgttaat tgatataacc gaattgaagc
      661 ggaagtcatc cgcaatttac cggaaaggaa aaaaatgaag aagcaggaca gaaaccgcct
      721 gtcgaagaaa gacagacggc tgattaaaaa ggcgatgctg aaagccgccg ccaaaggcag
      781 cgatgaggtt tacagaatcg cgccggggtt gaaagacggc tttgaattac ttggaaagca
      841 gcccgattaa atattcgtca tcggtatttg gctccgattc ttcgggtttt tgatgaagtg
      901 ttcggatgaa taccgccaat tctccggctt gtcctttggc cgtactgccg cttaagcgga
      961 tagaaccgct gcggataagc tctttggcga gtatgaagga taagtcggac ggcatttttt
     1021 tactccgtcg gccgttgtgt ggaaacccgg ttgcaacggg gtgacggcaa atcggaaaga
     1081 cggctgaccg cccggacaga cgggcggccg ataaagaaaa acccgcacgg ggcgggtaat
     1141 cccccctgaa ttgcagggaa gcggttcagg taacggcgaa aggcgattat gaatcaaaaa
     1201 caaacgcaat gcaaacaaat tgtcgattac atccgtaaca agggatgcat cacatccctt
     1261 gaggcttatc agaacctgaa ggtgacgcag cttgcggcac ggataaccga cttggaaggc
     1321 aggggcttcg tgtttgccaa gccgcgcatg aaggcgggcg gccgcgggaa gcctgttacg
     1381 cattattcga ttgtcaaaaa cggagcggaa gtatgagtgc gaggctgatg gggatggctt
     1441 tcaaaacggg tatcccgagg gggcagcgtt ttgttttggt aaagttgtgc gactgcgcca
     1501 acgacgaggg cttgtgttat ccgtcgcaag aaacgctggc ggaagatacg ggcttcgccg
     1561 aaaccgccgt acggcagcat atcaagtggc tgaaggataa caatttcatc aagtccgccc
     1621 ggcggcagag ggggcgggag aggaagtccg acatctaccg catcaacgtc gccctgcttg
     1681 aaaaatgcta tgcggaggcg gcaaaacgga aggcggcgcg gcaggcaaaa atgtgggaag
     1741 aaccattgga ttacgaaccg tcggattacg aaccgtcgga ttttgacgct aagaaccatc
     1801 agattttgag cggcgaaccg tcagattttg cgctaagaac catcggattt tgcgctaaga
     1861 accatcagat tttgagcgat gaaccatcag attttgacgg ttccttatat gtagaaccgt
     1921 cagtagaacc gtcagtagaa ccgtcagtag aaccgtcagt agaaccgtca ggatcaaatg
     1981 cgcgcggcgc gcgcgcccct gccggaccgc accctgcgaa accgcaaacg gcgcctcccg
     2041 aaa
//
LOCUS       DAXFWM010000127         2035 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000127 DAXFWM010000000
VERSION     DAXFWM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.127"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            64..963
                     /locus_tag="ACFI8B_002178"
     CDS             64..963
                     /locus_tag="ACFI8B_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFC3835285.1"
                     /translation="MKYIPNSFQIANAVVDDFLCRMSGNAWKCYAVIVRKTTGWQKEI
                     DYISVSQFKNLTGIKTDVTVADALKELVELNLIASVKRHGQVTGYRINMPEPSPENGG
                     TPPPENGGTATPKNWVHPKNGTTPKNWGVLPPENGGTTTPKNWGSTKHTTKPTNTKHS
                     ISASAAADAPLSAEPPESGKRAPAAKAKKTGRHETELSLLAAYGITGQAAEDFLTVRK
                     AKRQPLTETAMRLIAADAEKCGMTALQAAEYAIASGWGSFRADWLQNKTFGRSGNRGG
                     LTHNQTAAVPDAGSYGDMPTTDF"
     gene            978..1757
                     /locus_tag="ACFI8B_002179"
     CDS             978..1757
                     /locus_tag="ACFI8B_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3835286.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRREAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGEAGAVGIFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLMP
                     MLVLTNLTAEAFRENTDARIRDRLRDGGGKLIPFDWESYRA"
     gene            1827..>2035
                     /locus_tag="ACFI8B_002180"
     CDS             1827..>2035
                     /locus_tag="ACFI8B_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835287.1"
                     /translation="MREGAECGRAGEALPANQSLRRRGVSDGIGGGSRKKDGGAWGIS
                     PRNAPNLSGKAGKTLWEYPSLPEIE"
BASE COUNT          475 a          562 c          674 g          324 t
ORIGIN      
        1 ctcgattgtt gaaaacgggg tggaagtgtg ggcgcaagac caatgggaat ggaaggacgg
       61 taaatgaaat atattccaaa ttcgtttcag atagcaaacg cggtagtgga cgatttcctc
      121 tgccgaatga gcggcaacgc gtggaaatgc tacgccgtca tcgtgcgcaa aacgaccggc
      181 tggcaaaagg aaattgacta catctctgtt tcccaattta aaaacctgac cggaatcaaa
      241 acagacgtaa cagttgccga cgcgctgaaa gagcttgtgg aattgaacct gattgcctcc
      301 gtcaaacggc acggtcaggt aaccggctac cgcatcaata tgcccgaacc gtcccccgaa
      361 aatgggggta caccaccccc ggaaaatggg ggtactgcca cccccaaaaa ttgggtacac
      421 ccaaaaaatg ggactacccc caaaaattgg ggggtactac ccccggaaaa tgggggtact
      481 accaccccca aaaattgggg gtctacaaaa cacactacaa aacccactaa tacaaaacac
      541 agtattagcg catccgcagc ggcggacgcg cccctttctg ccgaacctcc cgaaagcgga
      601 aaacgcgccc cggcggcgaa ggcgaaaaaa accggcaggc acgaaaccga gctttcgctg
      661 cttgccgcct acggcatcac ggggcaggcg gcggaggact tcctgaccgt ccgcaaggca
      721 aaacggcagc cgctgacgga aacggcaatg cgcctgattg ccgccgatgc ggagaaatgc
      781 gggatgacgg cgctgcaggc ggcggagtac gccatcgcca gcggctgggg cagcttccgc
      841 gccgactggc tgcaaaacaa aactttcggc aggtccggaa accgcggcgg tctgacgcac
      901 aaccaaaccg ccgccgtgcc ggatgcggga agctacggcg atatgccgac gacggatttt
      961 tgaggggggg ttcggatatg gctttgagga acgcgtctga tttcttgggg gcttacggcg
     1021 gcggcgtgcg ggtcgagcgg aggcaatgcg cggaacacgg cggatacgcg gcgaaaagcg
     1081 ttttgcgcgg cgtgtggacg ggctgcccgg cctgccggaa gctggaggcg gcggacgaaa
     1141 tggcggcata cgcggaaacg ctgcgccgcg aggcgatgcg cgacgcgctg gaaaaacgca
     1201 tcgggcgttc gggcatcgcc ccgcggttca gaaactgccg gattgaaaac tacgccgtca
     1261 gcgattcgat cccggggatg gcgagggcga aggcggccgc cgccgagtat gcggcaaact
     1321 tcgccgatgt gttgcagacg gggcggagca tgattttttc gggcaggagg ggcacgggca
     1381 aaaaccacct tgcctgcggc atcgcccgcg aagtcatcgc cgccggcaaa agcgcgctgg
     1441 tcatcacggt gggcgatatg ctgcggacgg tcaaggacag tttcggcggc ggcggcgagg
     1501 cgggggcggt cgggattttc gtgaagcccg atttgctggt gctggacgag ttcggcgcgg
     1561 gcagtctgtc ggaaacggac gggcggattt tgttttccgt cgtcaacgcc cgctatgagc
     1621 ggctgatgcc gatgctggtg ctgaccaacc tgacggcgga agccttccgc gaaaacaccg
     1681 acgcgcggat cagggacagg ctgcgggacg gcggcggcaa gctgattccg ttcgactggg
     1741 agagctaccg tgcgtgaaac ctgctttttc tgcaagtacg cggattttaa aacccaaccg
     1801 gacacgccgg tgcgcggttt tgcgaaatgc gcgaaggcgc ggaatgcgga agagcgggcg
     1861 aagcattacc cgcgaaccaa tccctgcgcc gccggggcgt ttcagacggc atcgggggcg
     1921 gcagtcgcaa aaaggacggc ggcgcttggg gaatatcccc ccgcaatgcg ccgaatttga
     1981 gcgggaaggc gggtaaaacg ctttgggaat atcccagcct acccgagatt gaaaa
//
LOCUS       DAXFWM010000128         1980 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000128 DAXFWM010000000
VERSION     DAXFWM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.128"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(121..675)
                     /locus_tag="ACFI8B_002181"
     CDS             complement(121..675)
                     /locus_tag="ACFI8B_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835288.1"
                     /translation="MNTMNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFE
                     LLIDEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSG
                     RMSRKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMK
                     EIFDEILRIENLDRKNLPSNIMVK"
     gene            complement(762..1811)
                     /locus_tag="ACFI8B_002182"
     CDS             complement(762..1811)
                     /locus_tag="ACFI8B_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFC3835289.1"
                     /translation="MEFAKQRYIIKPGAIHVAQQFDLKVTTKQSPFSDGYDMARAALA
                     LGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRKHHLVWGEYEILSSENDRF
                     KNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGIRYGLTLSNELFGMAMSDN
                     PYAQSRLLQIEKQMSEIEEFFETVKKQVHAQIDSLAAAGMKITLIQNNNPVHIRLNVL
                     RAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLYNGGRMMRKLLNDLYIAVM
                     ETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWVYESQPSLVYIQRKMNQED
                     LNKLVDIVRDNGLSG"
BASE COUNT          612 a          465 c          295 g          608 t
ORIGIN      
        1 ccgaagttga cgaaagccga tgaatccgcc ggcggcatcc actactgtaa cggggcgtta
       61 cagtcacaaa aaaaccatca caaaagtgat ggcaaatgat ttttaaggct attaaatatc
      121 ttacttaacc atgatattac taggaagatt tttgcgatcc aaattttcaa ttcttaaaat
      181 ttcatcaaaa atttctttca tcgaaaacaa ttctgccaag tctccggcaa tctgcctacc
      241 tcggcaaaaa tactgtctgc aatcaaaata aaccttcata tgtaataacc gttcatcaag
      301 gctggcattc gtatcatcca acgccgtact aatatcggcc catttaaaaa acttctttct
      361 cgacatacgg ccactggaag aaacaacatt tttgaaaaaa atcatattcg gaatatagta
      421 tttttttaaa tccaaaccag ccaaactttt attactcaat ttcagatgtt ctcttactat
      481 aggatatatg agattaaggc cctgacatgc ttcatgaaac gactgagctt catcaattaa
      541 tagctcgaaa tcaaccaatt tcaatttaat ttctttttct aaatcctcaa tcccccttac
      601 ccttaaaagc cttttgtatt cccaaaattt taagtatttt ttcatacctg atatttgagt
      661 attcatagta ttcatatcac atcataaaaa aatccataca aacaagggcc aatcaatacc
      721 atcttaaaac tcgattgtaa ccccacgttc caatttcaca actagcccga taatccattg
      781 tcacgaacaa tatcaaccaa cttattgaga tcctcctgat tcatttttct ctggatataa
      841 accaaagagg gttgtgattc atacacccaa acagacaatg gaattctagg cagaatctct
      901 tgctcaacag cagatttcaa cttcgacaac tgctctgggt caagcagact gtcacgtcga
      961 atgctcttga tagccctggt ctccattacc gcaatatata aatcattcag tagctttctc
     1021 atcatccgac cgccattata caacgtgtca ttacattgtt tattagccat catgcctttg
     1081 atattaagtg tcttaacggc acatacaaaa ctatcgtaat cccgcaacaa tttaaccagt
     1141 ttgaaaccat aagcccgcaa tacattcaat ctgatatgaa cagggttatt gttctgaatc
     1201 aaggttatct tcatacctgc agcggccaaa ctatcaatct gagcatgaac ctgttttttc
     1261 acagtctcaa aaaattcctc aatttcggac atctgttttt caatctgcaa taaccgactt
     1321 tgggcataag gattatcgga catagccata ccaaacaact cgttactgag tgtcagacca
     1381 tatctgattc ccggccaacg tgggccgttc ttcttgttgt tattgccctc ccacatacgg
     1441 ataccttgct tggtatgcat aaaaaaacca tcatcaccgg aagaggtcag gctgccaata
     1501 tttttaaacc ggtcgttctc agaagaaaga atttcatact ccccccaaac caagtgatgc
     1561 tttcgacctg tctccttttg tagatgttgc ttgagcagca attcatgtgc tttaaactct
     1621 tcccactgtt ggaactgttc gtcatcctca gtcaactttt gattgacctt atcccccaaa
     1681 gccaaagcag cacgagccat atcgtaaccg tcactgaaag gtgactgttt tgttgtgact
     1741 ttgagatcaa attgctgtgc aacatggatc gcaccaggtt tgatgatata tctttgtttt
     1801 gcaaattcca tttttaaatc cttataagtt attaaaaaga gcttcataaa gcactgttgt
     1861 tgcatatcag aggctaatta gtcatgtaca ccatcatatc tgtcctttgc cgatgactgt
     1921 aacgccccgt tacatccgct tcgaagtaat agatttattc gtctttcaaa aattcatcaa
//
LOCUS       DAXFWM010000129         1905 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000129 DAXFWM010000000
VERSION     DAXFWM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.130"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(75..257)
                     /locus_tag="ACFI8B_002183"
                     /pseudo
     CDS             complement(75..257)
                     /locus_tag="ACFI8B_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(425..1018)
                     /gene="ribA"
                     /locus_tag="ACFI8B_002184"
     CDS             complement(425..1018)
                     /gene="ribA"
                     /locus_tag="ACFI8B_002184"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC3835290.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            complement(1011..1685)
                     /locus_tag="ACFI8B_002185"
     CDS             complement(1011..1685)
                     /locus_tag="ACFI8B_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835291.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
BASE COUNT          480 a          557 c          435 g          433 t
ORIGIN      
        1 ctccgttttg ccgacaatgg ccgttgcgtc aaaagtcaac ttggatatgt ccaatcctac
       61 ggcgttacgc atgggattac ccttatatgc ggatatttgc ctgcatattc gtcgcaaatc
      121 ttcccgcagc cgtccggcga accgtcatcg accaaaatca tttcataatc ggcaaaattt
      181 tcggcaagca cggaatccac gcagcagcga aggtattttt ccacattgta aatagggacg
      241 atgatagaga aaatcataaa tatcaatacg ttgtattaag atgtttgcgc gtatgcctca
      301 aacccgcgct cgcaatgcgt ttgcatccgc accctgcaac ttgataaaac ttattgcttt
      361 atcaagctat ggaaacctgt ttcccgaaag acggcgcagg atgcccgttc cctgcaactt
      421 tgccttattc cgacatcaaa tgccccagct tgtctgcttt ggtttggaga taacgctcgt
      481 tttccagatt ttccccgacg tgcaggggaa tgcgttcgac cacgttaatc cccgcatctt
      541 tcagggtttg gattttttcg gggttgttgg tcaacagttt gaccgagcgg atgcccagat
      601 attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc ccgagtgcca
      661 aattggcttc aacggtatcc ataccttgtt cttgcagatg ataggcgcgg attttgttaa
      721 tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc ccctctgcct
      781 gtaccgccct cattgccgct tcaagttgcg gtccgcagtc gcattttctc gagaacagcg
      841 cgtcgcccgt cagacattcg gagtggatgc gcgtcaacac cggattgccg tctgaacaat
      901 tgccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaaaccg tgcatcgtaa
      961 atacgcccca ttcggtcggc agacggcagg aagcgacatg gtctaataat tcagacattt
     1021 tcatctcctt ctgcggcagt gatgcccaaa agttgcttca aaggttcgga caaagccaag
     1081 gcaagagcca cccattccgc ctgcgccgcc gtatccgcac actctgcgca ttcaaattcc
     1141 acatgaacca cgcccaatac accgccgctc tccgtgcaaa cgggaatgga aatttgcgcc
     1201 gccgaagcat gattgcgttc tcccgaaagc tcacccaaat tcaaccaacg gcgtacatcc
     1261 gaggcaacat tcatccaacc gctttgcgcc gaacggcagg caagcgaaac atcggtaata
     1321 tttccatcct gctcccatat gttttccaaa ccttcgccct gttgggacag gcacaccagc
     1381 cggagggcgg cgttttcaga cggcatcaag gcataaaccg ccgcgctccg cacacctgtc
     1441 gagcgtgaaa acaccgaatc caaagccata aaaagccgtt tcagggcatc ttcgcggaca
     1501 tcatcgcacg gcaggtaatc ggcaagtttc caaccctcat cattgcacca caaaacagaa
     1561 cggtcaatcg aagccatccc catatccatt accgtctgcg ccgtcagata cgccgcccga
     1621 acctcgtcaa gcggcagctt caaaccctga gtcagcagaa aatccttaat caaagcagca
     1681 ggcataccca aattccgtca ataaaaacaa aaaaccgccc gattccgatg ccaggcggac
     1741 gaatagcgga ttttaccgct gcaaagcata agcctcaact attacgtcca aagccttgcg
     1801 caatcggaca aaatctgtat aatctccacc cttatgcgga cgtggcgaaa ttggtagacg
     1861 caccagattt aggttctggc gccgagaggt gtgagagttc gagtc
//
LOCUS       DAXFWM010000130         1842 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000130 DAXFWM010000000
VERSION     DAXFWM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.131"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..305
                     /locus_tag="ACFI8B_002186"
     CDS             <1..305
                     /locus_tag="ACFI8B_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835292.1"
                     /translation="RGAGAGKYPYRHCRYTVTLKPAIPAQAGIRICRDSLKSGGGGMV
                     LQINDVDTNSGEFAIYTAQDASVKLDLLKMELKHCKEMLEQKDKEIELLRKLTETV"
     gene            394..963
                     /gene="rsmD"
                     /locus_tag="ACFI8B_002187"
     CDS             394..963
                     /gene="rsmD"
                     /locus_tag="ACFI8B_002187"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC3835293.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1086..1337
                     /locus_tag="ACFI8B_002188"
     CDS             1086..1337
                     /locus_tag="ACFI8B_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC3835294.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1434..1517
                     /locus_tag="ACFI8B_002189"
     tRNA            1434..1517
                     /locus_tag="ACFI8B_002189"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1468..1470,aa:Tyr,seq:gta)
     gene            1548..1621
                     /locus_tag="ACFI8B_002190"
     tRNA            1548..1621
                     /locus_tag="ACFI8B_002190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Gly,seq:tcc)
     gene            1631..1705
                     /locus_tag="ACFI8B_002191"
     tRNA            1631..1705
                     /locus_tag="ACFI8B_002191"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1663..1665,aa:Thr,seq:ggt)
     gene            1754..>1842
                     /locus_tag="ACFI8B_002192"
     CDS             1754..>1842
                     /locus_tag="ACFI8B_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3835295.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTA"
BASE COUNT          514 a          424 c          477 g          427 t
ORIGIN      
        1 ggcgcggggc aggtgcgggg aaatacccgt accgccattg ccgatacacc gtaaccctaa
       61 aacccgccat tcccgcgcag gcgggaatcc ggatttgtcg ggattcgctc aaatcgggcg
      121 gcggcgggat ggtgttgcag attaacgatg tggataccaa cagcggggaa tttgcaatct
      181 ataccgctca ggatgcatcg gtaaagctgg atttgttaaa aatggaatta aaacactgca
      241 aagaaatgtt ggaacaaaaa gacaaagaaa tcgagttgct ccgcaagctg accgaaaccg
      301 tttaaacaga tatgccgtct gaaaaaagtt ttcaggcggc atattcttcg acaggtcttg
      361 tataataccg tttgaacttt caggcttttg attatggcgg caggcaaaca taccaaacac
      421 agcaaccggg tacgcattat cggcgggcaa tgccggggca ggaaattgag ttttgcatcc
      481 gccgacggac tgcgcccaac ccccgacagc gtgcgtgaaa agctgtttaa ctggctggga
      541 caggatttga cgggaaaaac ggttttggat ctcttcggag gcagcggcgc actcggtata
      601 gaagccgctt cgcgcaacgc caaacgcgtg ctgatttcgg ataacaaccg ccaaaccgtg
      661 cagaccttgc agaaaaacag tcgcgaactg ggtttggggc aggtgcaaac cgtctgttcg
      721 gacggcattg cctatttggc aaacctaaaa gagaaattcg atgtcgtctt ccttgatccg
      781 ccgtttgcgt ggcaaagttg ggaaagtctt ttcaatgtat tgggcacacg cctgaatgac
      841 ggcgcatatg tttatatcga ggcaggcagg cagccggaca aacctgattg gctgacggga
      901 tatagggagg ggaagtcggg gcagggtaca tttgaattaa gggttttcca agtggctgaa
      961 taatatgcgc tttgataatc atttccgggt tgtaaacatt cgtttgcaac cgtccggttc
     1021 aaaaaaacct tgtgctataa tccgcgcccg cccggttttg ataatttagt ggaaaaggaa
     1081 aagaaatgtc gctttttatt accgacgagt gcatcaactg cgacgtatgc gaacccgaat
     1141 gccccaatga tgccatttcc caaggcgagg aaatttacga aatcaacccc aacctctgca
     1201 cgcagtgcgt cggacactac gatgagccgc agtgccagca ggtttgcccg gtggactgca
     1261 tcctgattga cgaagaacat cccgaaaccc atgacgagtt gatggcgaaa tacgaaaaga
     1321 ttatccagtt taaataaatt ctttttaaaa catcaaatta tgtctgtttt gaaataaaat
     1381 caaaaaaaaa cttgacggaa aagcaagccg ctaataaact agtgttctct tttggaggga
     1441 ttcccgagcg gtcaaagggg gcagactgta aatctgttgc gaaagcttcg aaggttcgaa
     1501 tccttctccc tccaccaaaa ttcttacttg gggcagtagc gagtaatgcg ggtgtagctc
     1561 aatggtagag cagaagcctt ccaagcttac ggtgagggtt cgattccctt cacccgctcc
     1621 aaacaattag gcccatgtag ctcaggggta gagcactccc ttggtaaggg agaggtcggc
     1681 agttcaaatc tgcccatggg caccatctct cgattattca tttctttaag gcttagatat
     1741 ataggatatt gccatggcta aggaaaaatt cgaacgtagc aaaccgcacg taaacgttgg
     1801 caccatcggt cacgttgacc atggtaaaac caccctgact gc
//
LOCUS       DAXFWM010000131         1803 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000131 DAXFWM010000000
VERSION     DAXFWM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.132"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..187
                     /locus_tag="ACFI8B_002193"
     CDS             <1..187
                     /locus_tag="ACFI8B_002193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835296.1"
                     /translation="AGTKKPPFGRLFYEHKKIIKSRKHHVDFLKSPCRFPNTGGQAYM
                     MFIFWVLKKPASRGLCF"
     gene            complement(184..744)
                     /locus_tag="ACFI8B_002194"
     CDS             complement(184..744)
                     /locus_tag="ACFI8B_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835297.1"
                     /translation="MDIKTEEAYRLSFQHIQSHDVLAACRTVANYELSQPRPRGLFSG
                     ISAQEYWESYPCNEDAEILKSILLDSPEILDGISGGDMEMARIMAAFNFIWGLSHFPK
                     WLHRHEFASTPLDDSAVPAMLLFHAQSRQELKEYEEVELSCCPDGCEFCKSEDGKIYK
                     SSDAPVLPHAHCTHEQGCRCCYLPVI"
     gene            complement(744..1442)
                     /locus_tag="ACFI8B_002195"
     CDS             complement(744..1442)
                     /locus_tag="ACFI8B_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC3835298.1"
                     /translation="MNRVDRIKNLIADRFNGNQAEFSRAINKAPAQINQWLNGYRNIG
                     DGVAAQIEIALGLPRGWVDGNDKPGIPVDSIKSNATVIGSVDAWDSKTPLSDDDCEVP
                     FYKDVCLSAGNGFSDEIEDYNGYKLRFSKSTLRRHGINPDDVVCVSADGDSMEPVFPD
                     GATLGINTADKMIKDGKIYAINHSGLLRTKILQKLPDNQVRIKSYNPEYKDETASLDS
                     LTVIGRVFWWSVLD"
     gene            1541..1786
                     /locus_tag="ACFI8B_002196"
     CDS             1541..1786
                     /locus_tag="ACFI8B_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3835299.1"
                     /translation="MTEIEMDLRDYCAIRGNQSDLARKTGISPSFIHQIAKGLKPVPI
                     QSAALIEKSTNGRVTRKEMFPDTWHLIWPELADDQPK"
BASE COUNT          484 a          422 c          396 g          501 t
ORIGIN      
        1 cgcaggcaca aaaaaaccgc ctttcgggcg gcttttttac gaacataaaa aaattataaa
       61 gtcaaggaag caccatgttg acttcctgaa atcaccatgc cgatttccta acaccggcgg
      121 gcaagcctac atgatgttca ttttttgggt attaaaaaag cccgcatcgc gcgggctgtg
      181 tttttaaata acaggcagat agcaacatcg gcaaccctgc tcatgcgtgc agtgtgcgtg
      241 tgggagaacg ggtgcgtctg atgatttgta tatcttgccg tcctccgatt tacaaaattc
      301 acaaccatcg gggcaacaag acagctcgac ttcttcgtac tcttttaatt cctgacgcga
      361 ttgtgcgtgg aaaagcagca ttgcagggac ggcagaatca tcaagaggcg ttgatgcaaa
      421 ttcatgccgg tgaagccatt tcgggaaatg actcaatccc caaataaaat tgaatgcagc
      481 catgattcgc gccatttcca tatcgccgcc gctgattccg tccagtattt caggggaatc
      541 taaaaggata gatttcaaga tttcagcgtc ctcattgcac gggtagctct cccagtattc
      601 ttgagcggat atgcccgaaa acagcccgcg cggtcttggc tgcgataatt cataatttgc
      661 aaccgttcta cacgccgcca atacgtcgtg tgattgaatg tgttgaaagc tcaatcgata
      721 tgcttcttct gttttaatat ccattagtct aatacactcc accaaaatac cctgccaata
      781 acggttaggc tgtctaaaga ggcggtttcg tctttgtatt ccgggttgta gctcttgata
      841 cggacttgat tatcaggcag cttttgcaag atttttgtac gcaacagccc actgtggttg
      901 atggcataga ttttgccgtc tttaatcatt ttatcggcag tattaatacc cagtgtagcc
      961 ccgtcgggga ataccggctc catactgtct ccgtcggcag atacgcagac aacatcgtca
     1021 gggtttatgc cgtgccgtct taaggttgat tttgagaaac gcagtttata gccgttgtag
     1081 tcttcaattt catctgaaaa gccgttaccc gccgacaaac aaacgtcttt ataaaatgga
     1141 acttcgcaat catcgtcgga taacggggtt ttgctatccc aagcgtcaac agagccgata
     1201 acggtggcgt ttgatttgat ggaatcaaca gggatacccg gcttgtcgtt tccatccacc
     1261 catccgcgcg gtaatccaag cgcgatctca atttgggcgg ctacgccgtc ccctatattt
     1321 ctgtatccgt taagccattg atttatctgg gcaggagctt tattaattgc ccgagaaaac
     1381 tcagcttgat tgccgttaaa cctgtcggct atcagatttt taattctatc tactcggttc
     1441 atttcattac cccttcaaat aggtataaat ataaagcata gctacattta tcaatgctac
     1501 tattttatat ttagctatgc tatactcacg ctaaattgat ttgacagaga tcgaaatgga
     1561 tttaagagat tattgcgcga tacgcggcaa tcagtctgat ttggcaagaa aaacaggaat
     1621 ttccccgtct tttatccatc agattgccaa aggattgaag cccgtcccaa ttcagtcggc
     1681 ggcgttgatt gaaaagtcaa caaacggcag ggtaacacga aaagagatgt ttcctgatac
     1741 ctggcatcta atctggccgg aattggcaga cgaccaaccc aaataaaaag cccgtcgggg
     1801 atg
//
LOCUS       DAXFWM010000132         1777 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000132 DAXFWM010000000
VERSION     DAXFWM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.133"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            159..938
                     /locus_tag="ACFI8B_002197"
     CDS             159..938
                     /locus_tag="ACFI8B_002197"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC3835300.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            1147..>1777
                     /locus_tag="ACFI8B_002198"
     CDS             1147..>1777
                     /locus_tag="ACFI8B_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3835301.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTSKPKPQPHI"
BASE COUNT          518 a          594 c          362 g          303 t
ORIGIN      
        1 attaccgcct agtcggtaat gcatttttta gtagtatttg aaaaagtatg atgataaaaa
       61 cggttaagcc taactttttc ataaaatcca gcagagtaag gcagaaaaaa acacccaaat
      121 tggctataat cccgacaaac acactcaagg acaacaacat ggcagcctcg cccgaagcaa
      181 aattcaccga agaaaagatt ttgtgggtca aacaccacac gccgaaactc atcactttcg
      241 ccatcagccg tcccgaatcc taccgtttta aagccggaca gttctcccga ctcggtttct
      301 acgaaggtaa aggctttatt tggcgtgcct attccgtcgt ttccgcagaa tatgccgaca
      361 cgctcgaata ttttgccgta ctcatccaag acggccccat gtcggccctt ttcgccaaaa
      421 tgcaacaggg cgacaccatc ctgctcgata aaaatgccac tggctttctc ctgcccgaac
      481 gcttccccga cggcaaggat ttggtgatgc tctgtaccgg ctcgggcatc gcccccttcc
      541 tttccattct cgaacaaccc gaaatccggc agcgtttcga taccgtcaac ctgatacatt
      601 ccgtatcttt tcccgaagaa ttgattttca acgaccggct ggccgcattg tccgaacatc
      661 ccctggtagg cgaatacgga cactcgttcc gtttcgtccc tgttaccacc cgtgccgcca
      721 acccctcggg cttaagcgga aaacgcattc cggaactctt aaaaaacaac agcatcgaac
      781 aggcgctgca taccaagctt accccggaat ccacacggtt tatgatttgc ggtaacccgg
      841 aaatggtcaa agacactttc caaacgctgc tcgatatggg ttacgccatg caccgcaacc
      901 gcattcccgg tcaaatcatg atggaaaacg gcttctaaaa acgaacgctc ccaaccccta
      961 cccacgccca cacacaaaac aaaataaaaa acagcgatat acaaaaacca atttgcaaaa
     1021 acagacctca cgcctatact gccactcaca gcaagcaaca caaaaaagcg ggtacaaatt
     1081 agggtacaaa acacaagccc gaacacagaa gcaccccaac caaacccaaa ggcaaaggca
     1141 aacaaaatgg cggcacacaa caaactgacc caaaaacaaa tagaagcagc caaggcagac
     1201 ggcaaacaaa gcaagctggc cgacggcggc ggcttatatc tgctgctgca ccccaacggc
     1261 agcaaatact ggcggatgcg ctaccggcac ggcggatgcg aaaaaaccct agccttgggc
     1321 gtatatcccg ccgtcagcct caagcaggcc cgcgaacttg cccgcgccgc ccgggcgcag
     1381 accgccgccg gcatcgaccc cgtcgccgaa caccaccgca cccgtcccgg cagcgggcgc
     1441 agccttcccg aaatcgcccg cgcgtggtac ggcagccgtc aagggcaacg cgccgacaac
     1501 acactggaag gcgaccgccg ttcactcgcc tacctgaccg actactacaa agacaacacc
     1561 gacatcaacg acatcaccac cgccggcgta tcaaaattta tcgaacacct caaccgcatc
     1621 aacatccccg cctgcgcccg gcgcaccgtc caaatcctcg cccaaatatg ggactatgcc
     1681 gtccagcgcg gcatcattac cgacgaacgg cgcaaccccg caaccaccgc ccgcccactg
     1741 ctaaacacaa gcaaacccaa accccagccc cacatcc
//
LOCUS       DAXFWM010000133         1762 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000133 DAXFWM010000000
VERSION     DAXFWM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.134"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            517..1551
                     /gene="purM"
                     /locus_tag="ACFI8B_002199"
     CDS             517..1551
                     /gene="purM"
                     /locus_tag="ACFI8B_002199"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC3835302.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGLSVGAGDMVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT          464 a          409 c          445 g          444 t
ORIGIN      
        1 aaaaatatat aaatcatatt gttaatgatt attgggacaa ttcggtggca caaaatataa
       61 gctgtctgct tttcagcggg cggcttttta tctgcctatg tgtttcaaca cacaaaacgg
      121 cacataaagc accgccattt gttccgcacc gaatcggaag cgggtgcttc cgcccgcaat
      181 cggattcgcc gcccctaatg tcggggtttt aaccggaaag aagaaaatat tgattttgat
      241 gatggcatca aaaaaaccct tccgcccgtc tgccaaaagg aatggttaag aaaagcaatg
      301 cgtcttttga tggaacgcga tatataagaa attgagggga agggcaggcg ggtttctgcc
      361 cgttaatcct gcggacggcc ccgccaaacc cgacacgcgc gggcatatgg cggattgttt
      421 acaatattgt ctaaaaatct gaacctgcaa acgcgaaata aggtagaata cgccccgtta
      481 ttttaccgtc caatccccat ttaccaagga aaaacgatga gtacttcatt gagttaccgc
      541 gatgcgggcg tcggtatcga cgcaggcgac caactggtcg aaaaaatcaa accgtttgcc
      601 aaacgcacca tgcgtccgga agtattgggg gatttgggcg gttttggcgc attggtcgaa
      661 atcggcaaaa aatatcaaaa ccccgtattg gtcagcggta cggacggcgt gggtaccaag
      721 ctcaaacttg ccttcgattg ggacaaacac gatacggtgg gcatcgacct tgttgcaatg
      781 agtgtcaacg atattttggt tcaaggagct gaaccattgt ttttcttgga ttattttgcc
      841 tgcggtaaat tggatgttcc gcgtgcgacc gatgtcatca aaggcattgc ccaaggttgc
      901 gaagaatccg gctgcgccct gattggcggg gaaactgccg aaatgccggg tatgtatccc
      961 gttggtgaat acgatttggc gggttttgcc gtcggcgtgg tggaaaaaga gaatgtcatt
     1021 accggcctca gcgtcggcgc gggcgatatg gtattgggtt tggcttccaa cggcgcacat
     1081 tcaaacggct attcccttat ccgtaaaatc atcgaacgcg acaatcccga tctggatgcc
     1141 gagtttgata atggcaaaac tttacgtgaa gccgttattg cgccgacacg tctgtatgtg
     1201 aaaccgattc ttgccgcttt ggaaaaattt accattaaag gtatggcgca cattaccggc
     1261 ggcggcatta ccgaaaacgt gccgcgcgtg ttgcctaaaa acacggttgc ccaaatcgat
     1321 gccgaatcgt gggaattgcc caagctcttc caatggcttc aaaaggcggg caatgtggaa
     1381 acccaagaaa tgtaccggac ctttaactgc ggcatcggta tggtcgtcat cgttgctgcc
     1441 gaagatgccg atgcggttcg gtcattcctg agcgggcagg gcgagacagt gtaccgtttg
     1501 ggttgcatcc gagagcgtca agggaacgag catcaaaccc aagttgcctg acttccaagc
     1561 atcaaatgcc gtctgaattt gtttcagacg gcatttccgc atcttccaaa cgcgtatccg
     1621 caacacagtt ctgcaaactg atattttgcc cggtatggcg gcttaaactt cttcatcata
     1681 tttcgataat gctataaaat gatataatca taaaatattg tttattccta catcgtattc
     1741 atcctatttt ccgaaatgtg ag
//
LOCUS       DAXFWM010000134         1727 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000134 DAXFWM010000000
VERSION     DAXFWM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.135"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..180
                     /locus_tag="ACFI8B_002200"
     CDS             <1..180
                     /locus_tag="ACFI8B_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3835303.1"
                     /translation="DGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPLD
                     SLSVIGRVFWWSVLD"
     gene            340..879
                     /locus_tag="ACFI8B_002201"
     CDS             340..879
                     /locus_tag="ACFI8B_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC3835304.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            880..1239
                     /locus_tag="ACFI8B_002202"
     CDS             880..1239
                     /locus_tag="ACFI8B_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835305.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1256..1474)
                     /locus_tag="ACFI8B_002203"
     CDS             complement(1256..1474)
                     /locus_tag="ACFI8B_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835306.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
BASE COUNT          454 a          474 c          393 g          406 t
ORIGIN      
        1 gacggcaaaa tatacgcctt tgcccaagac ggcatgttcc gcgtcaaata cctgatacgg
       61 cagcccggca acagcgtgct gatacgcagc cacaacagcg gcttctatcc cgacgaaacc
      121 gcccctttgg acagcctgtc cgttatcggc agggtatttt ggtggagcgt gttggattga
      181 tgccgtctga aacggcaggg caagtatttt tcaaatatat ccgcccgata tgccgacggc
      241 gcggacagcc tgccgatacc ggataatttg cacaaattat ttcttgaaat aatgggccgg
      301 cggccttaat atacagaaaa ataacaacgg aaacagaaaa tgctgaacgc gtacgacgtg
      361 gcagatttct tcctttcccc ttttgaagaa gaggacgggg agcaaatctc caatctcaaa
      421 cttcaaaaac tcctgtatta cgcacaaggc tacgcccttg ccatacttaa ccgccccctg
      481 tttgccgaaa atatcgaaca ctggcagcac ggtccggtag tcccctgcat ttaccgcacc
      541 tacaaaaaat acggcggcag cccattgcct gcggcccata tcgaaccgga caaatatgcg
      601 gacgaagagt tggttgtcct caaccgtgtc cgtaaagagc agggctgcta caccgcttgg
      661 gcattacgca ataaaaccca tcaggaagcg ccgtggatac agacccggca gggcgaagtc
      721 atagggattg cgctgatggg ggaatatttc cgccatgcgc tgccgcagac ggattacaat
      781 ttcaatcttg aaaaactcaa aacagccgtt gaagacagct ttgtcagcgt cccgcatttc
      841 aacggcgccg acgacttgga aaaatggttg gagcagtaaa tgcaggtgct gcttggcgaa
      901 gacttcaaaa gagcgttgaa aaactacccc aaagaagacc gcagaaaaat tgcggaattt
      961 atcgcacacg tccaacagaa cggcttatca gggttacccg gcaggaacaa atccagcgac
     1021 aacgtaccgg cagacgatcc tcaatggctg gaaaaagtcc gatttgccca acggcacaac
     1081 ctttggcact atcacatcgg cattcccaaa tacaacggcg gcaggtatgg cgatttgaca
     1141 tccgcctaca tccttcacta taccctttgc gacggcttta tcaaaatcat cggttttgac
     1201 agacacccgc cttttatcct gcccgatatt cccaaataac cttgtcttac cccccttaag
     1261 acagcccctc gagataccgc ctgcaaatct ttgccaaagc ctgtacgcgg ctgcccccgg
     1321 ctttttccac ggcggcaagg ataagctcca cgtcttcggc acgcccctgc actgaaaact
     1381 gccggtattc gccgcttgcc agttttgcct ttgtcgccgc cgccgccgtt gccgcgcgca
     1441 attttttaga atgcccgctg ttggaatttg ccatttttaa atccttatag tagagtatgg
     1501 gaaagataag aggaagggca gcctccttaa ctgccctgca aatggttaat cgttaatcac
     1561 ttgttttgta tttccctacc acgctgggga gcttgcaagg aaatacaaaa tcacacagat
     1621 taggattttc ttaatcacat tgcctccttt ctctttcagg ttgccccgta tcggctgata
     1681 cggggtttct tcatttccta atctatgagt ttattatatc atatgat
//
LOCUS       DAXFWM010000135         1543 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000135 DAXFWM010000000
VERSION     DAXFWM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.136"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..235
                     /locus_tag="ACFI8B_002204"
     CDS             <1..235
                     /locus_tag="ACFI8B_002204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835307.1"
                     /translation="PMILNRVKCIYDYFQQGGRMNPTKQSKKSYESKRVLKHVSFNTE
                     KEANLLEFSNNLDFSKWVKEKLKHELELEKLKK"
     gene            734..>1543
                     /locus_tag="ACFI8B_002205"
     CDS             734..>1543
                     /locus_tag="ACFI8B_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3835308.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALAGRKGIDYSSAYSMVHQRFNVESIEGIPADKLPE
                     AVAYVHALTLHTGLTGEVLDREPLPAPQPALPISGNALADIAAMVYYGTRMIESGKDV
                     SAPLKQLGCRQAVTMWTVWHETRSILKRSAAALEVLRGYADKDASDRIAACL"
BASE COUNT          447 a          391 c          335 g          370 t
ORIGIN      
        1 ccctatgatt ttaaatcgag taaaatgtat ctatgattat tttcaacaag ggggaagaat
       61 gaacccaacc aaacaatcca aaaaaagcta cgaatctaag cgcgttttaa agcacgtttc
      121 atttaacact gaaaaagaag ctaatttatt ggaattttcc aataatttag acttctccaa
      181 atgggtaaaa gaaaagctga aacatgaact tgaattagaa aaactgaaaa aataatagaa
      241 atggcttgca tgaatcgtag atacaaatta taatttcaac acttgctgat tgcaggtaaa
      301 aaagaagccc aaaccaagct gtaacttgat ttgggcaggt ggaaagcaga gcggcaactc
      361 tctttccgcg tctatctacc atgaaataga aaggtcattc taccatgaat actgtccaag
      421 ttttaaactt tcaacaaagc tccgtccgtg cttcccccca aaatattgac gcaatagcgg
      481 aataatagta aagttcggtt cacaggtttg gtcgcctgtt tctttcgtgg ttattgccgc
      541 gcttgcggct tttgttttga taagagttcc ctgaagggtg tatgttaaac atacaccctt
      601 gctgaaaaag ccaacgggcg gactttcggg acatctccgg atgtgctgtt tccacgagca
      661 gtcgaccaac ctgaaacgtc cgcctacctc ttcttggtcg aagagaggct ttcaaacttt
      721 cgtggagctt aaaatgcaaa acaccatttc tgtattttct ttcaaatctc aaaatgtccg
      781 tactcaaatc ttgggggctg aaccttggtt ttgccttggc gatgttgcgg aaattcttca
      841 aattcaaaat gcccgtcagt tgccattaaa agaccagggc attcaaaaat ccagcgttgc
      901 aacaaaaaaa ggcaaccaag aattactttt catcaacgag ccgaatctct accgagtcat
      961 cttccgttca cgcaaagccg aagccgtcaa attccaagac tggatatttg aagaggttat
     1021 ccctcaaatc cgcaaaacag gcggctacca aattactccc aaaaccaccg ccgacgaccg
     1081 taccggattg cgccgggccg ttgccgcgct tgccgggcgc aaaggcatag actactcctc
     1141 cgcgtacagt atggtacatc aacgcttcaa cgtcgaatcc atcgaaggca tccccgccga
     1201 caaactgccc gaagccgtcg cctacgtcca cgcattgaca ctgcacacgg gcttgacggg
     1261 cgaagtcctt gaccgcgaac cgttacccgc gccgcaaccc gccctgccca taagcggcaa
     1321 cgctttggcc gacattgccg ctatggttta ttacggcaca cggatgattg aatcgggcaa
     1381 agacgtttcc gcaccgctga agcagctcgg ctgcaggcag gcggttacga tgtggacggt
     1441 ttggcacgaa acccgttcaa tcctgaaaag atccgccgcg gccctcgaag tgttgcgggg
     1501 atatgcggac aaagacgcct ctgaccgcat agccgcatgt ctt
//
LOCUS       DAXFWM010000136         1484 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000136 DAXFWM010000000
VERSION     DAXFWM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.137"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            14..508
                     /locus_tag="ACFI8B_002206"
     CDS             14..508
                     /locus_tag="ACFI8B_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFC3835309.1"
                     /translation="MTVQNTQTETVRTEAAPQQGGNTNPGYYKNRAFECVGFAQYLNF
                     NLGNAFKYIWRHKEKGGREDLEKALRYLERQRADAPKFKKLKCRRYEKMYAGLKDCGF
                     DGGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLLEKMPPEAGRAPHPESPMPPETA
                     GGGI"
     gene            685..834
                     /locus_tag="ACFI8B_002207"
     CDS             685..834
                     /locus_tag="ACFI8B_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835310.1"
                     /translation="MDTLLSIITALSFAGAATLAVWLLVEAADAVLRRKRDGKGEDDF
                     DGFGY"
     gene            863..1144
                     /locus_tag="ACFI8B_002208"
     CDS             863..1144
                     /locus_tag="ACFI8B_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835311.1"
                     /translation="MAEEMRTCKACGGTKPLEKGFNAVPRKEGGVYYYKSCKTCRNKA
                     VRQKRAEKRAAAGAGAMTAARLHGYIRAAHAACPILGAGLWTQPAGECA"
     gene            1135..>1484
                     /locus_tag="ACFI8B_002209"
     CDS             1135..>1484
                     /locus_tag="ACFI8B_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC3835312.1"
                     /translation="MRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAYRETVRRIAQG
                     AGAMPSEGAVAVYVRLIPKANKDGGANKTVIDLDNALKVALDALQGVAYHNDRQVRRI
                     AADYADEPVAGGGLA"
BASE COUNT          363 a          425 c          468 g          228 t
ORIGIN      
        1 aaggaaatac ggaatgacgg tgcaaaacac gcaaaccgaa accgtccgga cggaagccgc
       61 gccgcaacaa ggcggcaata ccaacccggg ctattacaaa aaccgcgcct tcgagtgcgt
      121 cgggtttgcg caatacctca acttcaacct cggcaacgcc ttcaaataca tctggcggca
      181 caaggaaaaa ggcgggcgcg aagacttgga aaaagccctg cggtacttgg aacgccaacg
      241 cgccgacgcg ccgaagttca agaaactcaa atgccgccgc tatgaaaaaa tgtacgccgg
      301 tctgaaagat tgcgggttcg acggcggcac ggaggccgcg ctgcttgccg tcatctccgc
      361 cgcttattac atccgcgacg gcgaagacaa ttttgcgtgg gcggccgcct gtgtcgaaga
      421 tttgttggaa aaaatgccgc ccgaagcggg gcgggccccg caccctgaaa gcccgatgcc
      481 gcctgaaacg gcgggcggag gcatttgacc cgccaacccg accgccgcca ttcccgcgaa
      541 agcgggaatc cggaatcccg gactttcaga taatctttga atattgctgt tgttccaagg
      601 tccggattcc cgcctgcgag ggaatgacgg aggcggcggg aatccgaccc cgacccataa
      661 aaccgaccga aaggaaataa aacaatggat accctgttaa gcatcatcac cgcgctgtcg
      721 tttgccgggg cggcgacgtt ggcggtatgg cttttggtgg aagccgccga cgcggttttg
      781 cgccgcaagc gcgacggcaa aggcgaagac gactttgacg gcttcggata ttaaacacat
      841 aaaaacaaaa ggaaaaatca aaatggcgga agaaatgcgc acctgcaagg cctgcggcgg
      901 aaccaagccg ttggagaaag ggtttaatgc cgtcccgcgc aaggaagggg gggtctatta
      961 ttacaaatcg tgcaaaacct gccgcaacaa ggcagtccgg caaaagcgcg cggaaaaacg
     1021 cgcggcggcg ggagccggcg cgatgacggc ggcaaggctg cacggataca tccgcgccgc
     1081 gcacgccgcc tgcccgatat tgggcgccgg cctgtggacg caaccggcag gggaatgcgc
     1141 gtgatacgcc ttatcctgcc ttaccccgta tcggcaaacc gatattggcg gatttggcgc
     1201 aacagggcgg tcaggagcgc ggaggcggcg gcgtataggg aaaccgtccg ccgtatcgcg
     1261 caaggggcgg gcgcgatgcc gtccgaaggc gcggttgccg tatatgtgcg cctgattccc
     1321 aaagcgaaca aagacggcgg cgcaaacaag acggtgatcg atttggacaa cgccctgaag
     1381 gttgcgctgg acgcgcttca aggcgttgcc tatcacaacg acaggcaggt gcggcgcatt
     1441 gccgccgatt acgccgacga gccggtcgca ggcggcggtt tggc
//
LOCUS       DAXFWM010000137         1431 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000137 DAXFWM010000000
VERSION     DAXFWM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.138"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..281
                     /locus_tag="ACFI8B_002210"
                     /pseudo
     CDS             1..281
                     /locus_tag="ACFI8B_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            321..692
                     /locus_tag="ACFI8B_002211"
                     /pseudo
     CDS             321..692
                     /locus_tag="ACFI8B_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            784..>1431
                     /locus_tag="ACFI8B_002212"
     CDS             784..>1431
                     /locus_tag="ACFI8B_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3835313.1"
                     /translation="MASASKIIGKYVKQVEVKNGVVTAQMASSNVNKEIKDKKLSLWA
                     RRQDGSVKWFCGQPVTRAKAKDADDVTDDAGTDNGGKGKIDTKHLPSTCRDAASAVCT
                     KHHAPISNTSKKSAVAGYCPNHGEWPENNASAGVASSGEIKGKYVQKVEVAKGVVTAT
                     MNSSGVNKEIQGKKLSLWARRENGSVKWFCGQPVKRTDAKADTVADDAGKDNGGKG"
BASE COUNT          455 a          418 c          344 g          214 t
ORIGIN      
        1 cttcaaaaat cataggcaaa tatgttaaaa gcgttacggt cacaaacggc gtcgttaccg
       61 cccaaatgaa tccaagcggc gtaaacaatg aaatcaaagg caaaaaactc tccctgtggg
      121 ccaagcgtga aaacggttcg gtaaaatggt tctgcggaca gccggttaag cgcgacgccg
      181 gcgccaaaac cggcgccgac gacgtcaaag ccgacggcaa agacaccgac aaaatcaaca
      241 ccaagcacct gccgtcaacc tgccgcgata aatcatctgc cgtttgcacg aaacaccacg
      301 cgccgatttc aaacacttcc aaaaaatcag ccgttgccgg gtattgcccg aatcacggca
      361 aatggccgaa agacaacact tctgccggcg tggcaaaccc caccgaaatc aaaggcaaat
      421 atgttcaaag cgttacggtc gcaaacggcg tcgttaccgc ccaaatgaaa tcagacggcg
      481 taaacaaaga aatcaaagac aaaaaactct ccctgtgggc caagcgtgaa aacggttcgg
      541 taaaatggtt ctgcggacag ccggttacgc gcgccgccgc cgacgccaaa gacgccaaag
      601 acgacgttgc cgccgacaac ggcaacggca acggcaaaat cgacaccaag cacctgccgt
      661 caacctgccg tgatccggca tctgccaaat aaggcaaatt aggccttaaa tttcaaataa
      721 atcagccctc accgaatatt acctgaaaac ggcgaatggc ccaaagacaa cgactctgcc
      781 ggcgtggcat ccgcttcaaa aatcataggc aaatatgtta agcaagttga agtcaaaaac
      841 ggcgtcgtca ccgcccaaat ggcttcaagc aacgtaaaca aagaaatcaa agacaaaaaa
      901 ctctccctgt gggccaggcg tcaagacggt tcggtaaaat ggttctgcgg acagccggtt
      961 acgcgcgcca aagccaaaga cgccgacgac gttaccgacg acgccggcac cgacaacggc
     1021 ggcaaaggca aaatcgacac caagcacctg ccgtcaacct gccgcgacgc agcatctgcc
     1081 gtttgcacga aacaccacgc gccgatttca aacacttcca aaaaatcagc cgttgccggg
     1141 tattgcccga atcacggcga atggccggaa aacaacgctt ctgccggcgt ggcatcctcc
     1201 ggcgaaatca aaggcaaata tgttcagaaa gttgaagtcg caaaaggcgt cgtcaccgcc
     1261 acaatgaatt caagcggcgt aaacaaagaa atccaaggca aaaaactctc cctgtgggcc
     1321 aggcgtgaaa acggttcggt aaaatggttc tgcggacagc cggttaagcg caccgacgcc
     1381 aaagccgaca ccgttgccga cgacgccggc aaagacaacg gcggcaaagg c
//
LOCUS       DAXFWM010000138         1396 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000138 DAXFWM010000000
VERSION     DAXFWM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.139"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..269)
                     /locus_tag="ACFI8B_002213"
     CDS             complement(<1..269)
                     /locus_tag="ACFI8B_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835314.1"
                     /translation="MMTESHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTD"
     gene            complement(290..496)
                     /locus_tag="ACFI8B_002214"
     CDS             complement(290..496)
                     /locus_tag="ACFI8B_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC3835315.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(462..>1396)
                     /gene="mafB"
                     /locus_tag="ACFI8B_002215"
     CDS             complement(462..>1396)
                     /gene="mafB"
                     /locus_tag="ACFI8B_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3835316.1"
                     /translation="SRADEAGKLIWENNPNQNWWGNRMDDVRGIIQGAANPFLTGFQG
                     LGVGAITDSAVNPVTYAAARKTLQGIHNLGNLSPEAQLAAASLLQDSAFAVKDGINSA
                     RQWADAHPNITATAQTALAVAEAAGTVWGGKKVELNPTKWDWVKNTGYEKPAARPMQT
                     VDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIQDRIRWTKSKFP
                     TEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRYDINNNI
                     YVLANPKTFKIKTMFKPNLGKEYYDGEFKKDMGN"
BASE COUNT          347 a          295 c          262 g          492 t
ORIGIN      
        1 caatcggtat aaaccctatt cacagtttta tcttgtattt ccttatgaat acttaatata
       61 ttgagatagt ttagaaattt tattttctca gattgaggta actcatcaaa accaagccaa
      121 taataaaaat tgttatatga ttttaaaaaa tcaacaattt ttatttttcc taaaaccaaa
      181 tcttcgacta actgggttgc ataaataata tcatttatga ttttaacagg taaattattt
      241 gatgggtaac catgggattc cgtcatcatt ggtttctcta ggtgcttatt tatctgattg
      301 gtaagccttt tgcgtaagct tctttcgcct ccgcaagtgt catttcatta ggaccaccat
      361 ctatattagt ttctcctgta ttttgccact gacaaacgtc acagatatca tagtccataa
      421 cttcagttcc gcaaacagga caatgtagcc atatttctcc gtcaatttcc catgtctttt
      481 ttgaattctc catcataata ctcctttcct aagtttggtt taaacattgt tttgattttg
      541 aatgtttttg gattggccaa aacatagata ttgttattga tatcatagcg cactcgtcta
      601 tgttccgtct gataacctaa tatcttgtcc gatgtctgtt ttgataacaa atcagacgcc
      661 attttttggt agtcttcaat ggttatatcg ccaaattctt ttccatgagc tttgaaatgt
      721 ccatttaaag atttctcagt aggaaacttg gattttgtcc atctgatgcg gtcttgaatt
      781 gttcctgtcg gagaaataga tgtatcaggt ttaacatatg gggcaggtaa gccgatattg
      841 ttatcagagt gtgtagagtg ttgctgcgta cttggtttcg gtggcttatt tttcccggcc
      901 atttccccgt ctacagtctg cataggtcgg gcagcaggtt tttcatagcc ggtattttta
      961 acccaatccc atttggtcgg gttaagttct acttttttac ctccccaaac cgtacctgca
     1021 gcctctgcta cggcaagggc agtttgggct gttgctgtta tattcggatg ggcatcagcc
     1081 cattgtctgg cggaattgat gccgtctttt accgcaaaag cactgtcctg taataggctc
     1141 gcggcagcaa gttgtgcttc cggacttaaa tttcctaaat tgtgaatacc ctgtaaagtt
     1201 ttccgtgctg ccgcataggt taccgggttt accgcgctgt ctgtaatcgc cccaactccc
     1261 aatccctgaa aacccgttag aaaaggattg gctgcacctt ggatgatgcc gcgaacatca
     1321 tccatacggt taccccacca attttgattg gggttgtttt cccatatcag ttttcctgct
     1381 tcatcagcac ggctga
//
LOCUS       DAXFWM010000139         1356 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000139 DAXFWM010000000
VERSION     DAXFWM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.140"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(12..290)
                     /locus_tag="ACFI8B_002216"
     CDS             complement(12..290)
                     /locus_tag="ACFI8B_002216"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3835317.1"
                     /translation="MAISDVFYQKQLKGNGLVQSLSRKGNCLDNAAMESFFGTLKSEC
                     FHTCKYDSVTESEAALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="ACFI8B_002217"
     CDS             complement(254..832)
                     /locus_tag="ACFI8B_002217"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC3835318.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAQNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..1238)
                     /locus_tag="ACFI8B_002218"
     CDS             complement(837..1238)
                     /locus_tag="ACFI8B_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3835319.1"
                     /translation="MARYRLHGESGIKRRKHTTKYSVEYKLEAIRPVTGQGMSQKAAA
                     DQLNLPECSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADYLKTKEELFA
                     ELAYLKAEAAVLKKLDALKEVRQKERNLSQG"
BASE COUNT          302 a          343 c          309 g          402 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactca gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttctgcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgaca agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcattcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtt
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa aatatagtga attaacaaaa atcaggacaa ggcggcggac cgcaggcagt
     1321 acaaatggta cggaaccggt tcgcccggtg cttcat
//
LOCUS       DAXFWM010000140         1349 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000140 DAXFWM010000000
VERSION     DAXFWM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.141"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            59..1333
                     /locus_tag="ACFI8B_002219"
     CDS             59..1333
                     /locus_tag="ACFI8B_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835320.1"
                     /translation="MEKPMKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTL
                     VLAKQGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSI
                     ERNINKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSS
                     SLAPAMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKS
                     GKDKGQGITANHYGNSDINFNKASPNITTMEFKGDVNIKIDRNGQEEAESNGFGFYSS
                     RKLGNKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGG
                     EGKVVKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYE
                     LTPDTKSIEKKKLNVRGCTRLSLNSTPIPQDF"
BASE COUNT          437 a          290 c          344 g          278 t
ORIGIN      
        1 gggggggggg cgtgtcaatg atttaagcat ataatgataa aaatcacata ccttcaaaat
       61 ggaaaaaccg atgaaacaaa agaagacagt tcagtgcatc ttacttggct tcgcggccgc
      121 ttctatgcac gcgcaagggg cggccgctgc aaacagtgga acgatagaga agacagacaa
      181 atacaccctc gtcctcgcaa aacaagggca agagaacaac tataccctta atggcggaac
      241 cgaagtcaaa cccttaaatt ccctcatcat tgccgccaac ggcggtacga acaacattac
      301 gataaaaggc aaattggcag acggtcctgc cgatgccccg ccgacgattg acaataactc
      361 gattgaaaga aatataaata aaaacggtta cacgtacgca tggcagaatt ggagcggcgc
      421 ggtgatgttg gtggatcaaa gctatgaggg agaaaataag gttacttttg aaaacgtaac
      481 catagccgcc cataacgcgc ctgccggcat tttgtctgat gataggcata aatccagtag
      541 cttggcgccg gcgatgcttg cctttaaagg gcgcaatacc atcaatatgg acgcggactc
      601 aaatgccaac tctagtaacg aaggtatcct attgcttaat aatggtgaaa aaatgggaga
      661 atatcgtctt gtttccgaag aaggctcgac gctgaatatc aacatcaaat caggaaaaga
      721 taaaggacag ggcattaccg ccaaccacta tggtaactca gacatcaact tcaacaaggc
      781 ttctccaaac atcaccacaa tggagttcaa gggcgatgta aacattaaaa tcgataggaa
      841 cggacaggaa gaggcggaaa gcaacggttt tggcttctat tcaagccgca aattaggcaa
      901 taagaagcag attccggaag gttctaagat ggaagcgatt ttccgtggga acgtcgatat
      961 cgttgcaacg ccggtttatg acgagcaagg caggcccaaa agcataggca gcgcgtttgc
     1021 gattgacggg aaatacagca aagtcgaggt tgtcggcggg gaaggcaagg ttgtcaaaat
     1081 caaaggcgat attttcgcct acaacggcgg cagcgtgagc gtgaaccttg ccaacaaaga
     1141 ctcttatttt gaaggggaag cccatatcgg gaaaaggagc tttgccaaag ggaaagatat
     1201 gtttgcctta accgtggatg cggacggata tgaattaacc cccgatacaa agtcgattga
     1261 aaagaaaaag ctgaatgtta ggggctgtac tagattatcc ctaaattcca caccgatccc
     1321 gcaggatttt tagctgccgg gacggtgtg
//
LOCUS       DAXFWM010000141         1285 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000141 DAXFWM010000000
VERSION     DAXFWM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.142"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            227..373
                     /locus_tag="ACFI8B_002220"
     CDS             227..373
                     /locus_tag="ACFI8B_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835321.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(750..1199)
                     /locus_tag="ACFI8B_002221"
     CDS             complement(750..1199)
                     /locus_tag="ACFI8B_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC3835322.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          406 a          310 c          191 g          378 t
ORIGIN      
        1 aaaataattt gagattttat taaaattcat gcatctaaat aagcagaact taatttcctc
       61 caaacgatcg gatatcttca aatgcacatt tcacagaaag gtcgtctgaa acccattttc
      121 agacgacctt tttccacttc gcaaaacaag aattcccacc cctctcccca aacatcgccc
      181 aaaccagtaa attccccata tttcaacaac tccttggaag caaaccatgt cccgtatcta
      241 cctacccctc ctatttccgc cccacatcgt cgaacgcggc cttttgtatt ttcagcagga
      301 caaggttcgc gatgtccaaa aaatttcctt agggcgttat cgggcgaagg tattcgattc
      361 ggaaaactat taggcatggt tgaagctgga tagtgatttg tacattaagg acggggctgc
      421 gattgccctt atgtctaaag gtgcaaacat gccttaaatt actaagccta taacgaattg
      481 gaaacaaaat gccgtctgaa aacatcttca gacggcatta taaaatctgt tcaccttttc
      541 agatgagtaa tgtacacccc tatacaattt ttgctaccat gcaccataaa tccacggcta
      601 aagataattg ggtttattct tttatccaga aacaatccag atacggatca cgggtcataa
      661 ctataggctt aatattacac gattctcatt ccatcaaggc ggaaaaccgc acaaatactg
      721 aaacactatc gatcgatttg taaacaagcc tacttaagta acttgcagtc cttatcattt
      781 cctttaaaat aatccagccc gtcactacac gaactggcgg acttcttgca aataaaggtt
      841 actagatttt cattcatctt aataataaaa ggatttttat ctttatctat ggctaccgcc
      901 ttcaacatga atttactgtc taaagccccg cgcgcgattc cattcaaacg gatacaaaag
      961 ccttctgcct ctttaatcgg caaacttggc catttggtag atgtttgttt aaatctccca
     1021 ttctgcagat aaaacttttc cataaaatgt gcattttcta acaaggctgc ccgcactgca
     1081 tttatctttg ctttctcaac ataattgcga tagctcggat aaacaattaa agcaagtaca
     1141 gacaatatca agaccactga tattaattca accagcgtaa acccccgatt atcagtcatt
     1201 actttacttc caataagaac agattattca acatatttct ttgaacagac ttactatccc
     1261 attcagcagt atgcatattt cccac
//
LOCUS       DAXFWM010000142         1223 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000142 DAXFWM010000000
VERSION     DAXFWM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.143"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..187
                     /locus_tag="ACFI8B_002222"
                     /pseudo
     CDS             1..187
                     /locus_tag="ACFI8B_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            323..>1223
                     /gene="mafA"
                     /locus_tag="ACFI8B_002223"
     CDS             323..>1223
                     /gene="mafA"
                     /locus_tag="ACFI8B_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC3835323.1"
                     /translation="MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADN"
BASE COUNT          359 a          288 c          267 g          309 t
ORIGIN      
        1 tgatgtattg gatgcaggcg aatttagcca tttccgtatc aatcacagca cacattttgc
       61 cgaacgacaa aaccatatta atggaattgg gaacttttgg aaccgggcaa aacgtcattt
      121 acgcaagttt gacggcattc ccaaagagca ttttgagccg tatttaaagg agtgcgaacg
      181 gcgtttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc
      241 gagtttatcc tagttgtcca ggacggcccc taatttattt acaattttga tacaatttgt
      301 ttttcatcaa aggagaaaat ctatgcgggc acggctgctg atacctattc ttttttcagt
      361 ttttatttta tccgcctgcg ggacactgac aggtattcca tcgcatggcg gaggcaaacg
      421 cttcgcggtc gaacaagaac ttgtggccgc ttctgccaga gctgccgtta aagacatgga
      481 tttacaggca ttacacggac gaaaagttgc attgtacatt gcaactatgg gcgaccaagg
      541 ttcaggcagt ttgacagggg gtcgctactc cattgatgca ctgattcgcg gcgaatacat
      601 aaacagccct gccgtccgca ccgattacac ctatccgcgt tacgaaacca ccgctgaaac
      661 aacatcaggc ggtttgacag gtttaaccac ttctttatct acacttaatg cccctgcact
      721 ctcgcgcacc caatcagacg gtagcggaag taggagcagt ctgggcttaa atattggcgg
      781 gatgggggat tatcgaaatg aaaccttgac gaccaacccg cgcgacactg cctttctttc
      841 ccacttggta cagaccgtat ttttcctgcg cggcatagac gttgtttctc ctgccaatgc
      901 cgatacagat gtgtttatta acatcgacgt attcggaacg atacgcaaca gaaccgaaat
      961 gcacctatac aatgccgaaa cactgaaagc ccaaacaaaa ctggaatatt tcgcagtaga
     1021 cagaaccaat aaaaaattgc tcatcaaacc caaaaccaat gcgtttgaag ctgcctataa
     1081 agaaaattac gcattgtgga tggggccgta taaagtaagc aaaggaatca aaccgacgga
     1141 aggattaatg gtcgatttct ccgatatccg gccatacggc aatcatacgg gtaactccgc
     1201 cccatccgta gaggctgata aca
//
LOCUS       DAXFWM010000143         1180 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000143 DAXFWM010000000
VERSION     DAXFWM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.144"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..286)
                     /locus_tag="ACFI8B_002224"
     CDS             complement(<1..286)
                     /locus_tag="ACFI8B_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3835324.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVTNHRLALAADEVFEGPAGPGGSCFGVRRKGRRGRGAVGKAVVFGI"
     gene            487..1047
                     /locus_tag="ACFI8B_002225"
     CDS             487..1047
                     /locus_tag="ACFI8B_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC3835325.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT          312 a          378 c          263 g          227 t
ORIGIN      
        1 gaatgccgaa gacaaccgct tttcctaccg cgccgcgacc gcgtctgcct ttacgccgta
       61 cgccgaaaca gcttccgccc ggcccggcgg ggccctcgaa aacctcatcg gcagccaagg
      121 ccaaacgatg gttggtaacc gtgcggattt tacggtaaaa cagtgccgcc gaatcgggat
      181 ggatacccaa aatatcggcg gcagaacggg cggtaacttc cggtacaaaa gaacggagcg
      241 gttctttttg tacttctttc tttaatttgc agtgtgttat cttcatattt cgagggtaac
      301 atatctgcca atctggtaca gacccaaaaa caaagcacgg ataacaccgc gctttttcat
      361 ccgccctgtt ccgcccgccc gttcgtcaac cgcctgcaaa cccaccgttt accggcagga
      421 aatgccataa atccccgcct atgttaggat aaacgccttt accggcccac acaaaggatt
      481 atcatcatga aaaaaatcat cttcgccgcg ctcgcagcgg cagccgtcgg cactgcctcc
      541 gccacctaca aagtggacga atatcacgcc aacgtccgtt tcgccatcga ccacttcaac
      601 accagcacca acgtcggcgg tttttacggt ctgaccggtt ccgtcgagtt cgatcaagca
      661 aaacgcgacg gcaaaatcga catcaccatt cccgtcgcca acctgcaaag cggttcgcaa
      721 cccttcaccg gccacctgaa atccgccgac atcttcgatg ccgctcaata tccggacatc
      781 cgcttcgttt ccaccaaatt caacttcaac ggcaaaaaac ttgtttccgt tgacggcaac
      841 ctgaccatgc gcggcaaaac cgcccccgtc aaactcaaag ccgaaaaatt caactgctac
      901 caaagcccga tggcggaaac cgaagtttgc ggcggcgact tcagcaccac catcgaccgc
      961 accaaatggg gcgtggacta cctcgttaac gccggtatga ccaaaaacgt ccgcatcgac
     1021 atccaaatcg aagctgcaaa acaataagcc ttgatgcgaa tcagcacaaa tgccgtcttc
     1081 atcaaaccgc cctgccaacc gtaagccgat atggcggagc acaacccgcc gaaagatatt
     1141 ttggcggcag gcggggcttg ccggaaagtc cggaaagtaa
//
LOCUS       DAXFWM010000144         1180 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000144 DAXFWM010000000
VERSION     DAXFWM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.145"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            160..636
                     /locus_tag="ACFI8B_002226"
     CDS             160..636
                     /locus_tag="ACFI8B_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835326.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLKQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
     gene            633..902
                     /locus_tag="ACFI8B_002227"
     CDS             633..902
                     /locus_tag="ACFI8B_002227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835327.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGSGYGNGYGNGYGNGYGGVGGGGS
                     GSGYGNGNGYGNGSGDGDGNGNGSGNGSGYGNGYGNGYSNGSGNG"
     gene            999..>1180
                     /locus_tag="ACFI8B_002228"
     CDS             999..>1180
                     /locus_tag="ACFI8B_002228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835328.1"
                     /translation="MNAAGGFLKLKDKAMKYYGTAAYGSPDWGMERYYAREDMRQALD
                     GWEAENRILHESGLIE"
BASE COUNT          341 a          288 c          353 g          198 t
ORIGIN      
        1 tggtgaaatc tgccgcgacg cagcagcgac atggaccact agaaaaagcg aaagcatcaa
       61 tcacaccgcc ttcactttta cagtgaaaaa agacggctta aaagagaagg taatgattgt
      121 tttgtaaaga aaccaacccc accccgaaag gaaacaaaaa tggaagcaaa taaatttgaa
      181 gtgaaaagtt tgtcagacct tataaaagtc tttgcaggca ttgctgccga tttcgaagcg
      241 gcaatgggtg taaaacgcgc cgacatttca accgaatttg acgaaccgca acatgagccg
      301 caaccgccgg taacagttgc cgagcaaaaa ggtatcaacg actttgccat cggcaaggaa
      361 gtcatcatcc gcacttattc ggcaggcgtt tggtttggtg tgttgaaaca aaaagcaggc
      421 aatgaagtga ttctgacaaa agcgcgccga atgtacagct ggtgggcaaa ggaatcaatc
      481 agcctgtcag gtgtcgcacg acacggcatc aggcaagacg gcagccaaat ttgcggcgag
      541 cttgattccg tatggctcga ggcgattgag attatcccag taacaggcgg cgcggctgaa
      601 tcaatccgca ccgcgctgga ggtcgcccag tcatgagtta tctagatcaa ccattgaagc
      661 acggctacgg caacggcaac ggtagcggca acggtagcgg ctatggcaac ggctacggca
      721 acggctacgg caacggctac ggcggcgtcg gcggcggcgg tagcggcagc ggctacggca
      781 acggcaacgg ctacggcaac ggcagcggcg acggcgacgg caacggcaac ggtagcggca
      841 acggtagcgg ctatggcaac ggctacggca acggctacag caacggcagc ggcaacggct
      901 aaaccttaaa accgcccgaa gctatatccg ccaagccggg gatagcacaa agcgatgaag
      961 tatagcactt caaagcgggg caacggcacg gcgaaacggt gaatgctgcg ggcggtttcc
     1021 tcaaacttaa ggataaagcg atgaaatatt acggcacggc ggcttacggc agccccgatt
     1081 gggggatgga aagatattac gcgcgggagg atatgcggca ggctttggac ggttgggagg
     1141 cggaaaaccg aatcctgcac gaatccgggt tgattgagat
//
LOCUS       DAXFWM010000145         1127 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000145 DAXFWM010000000
VERSION     DAXFWM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.146"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            74..418
                     /locus_tag="ACFI8B_002229"
     CDS             74..418
                     /locus_tag="ACFI8B_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFC3835329.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            372..1025
                     /locus_tag="ACFI8B_002230"
                     /pseudo
     CDS             372..1025
                     /locus_tag="ACFI8B_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          342 a          210 c          256 g          319 t
ORIGIN      
        1 ccgtatttta atgtcgtatt gttcctgtat atattagtga tgtgataagt atagtttatt
       61 ttttgaggtt tttatgaatt taccttttcg ggcaatggtg tctgatttag gtggcacact
      121 tttaacacct gaacatttag tgggtgatct taccattgat actctgagag tattagagca
      181 aaaaggcgta gatattattt tggcaacagg acgcaatcat accgatatgt catccattct
      241 tggaaaaatc ggtgcggaac gtgcggttat gattacatca aacggtgctc gtgtacggga
      301 tttacagggt aatttgcttt atagcaatag tttgcctgaa gaattagttc tcgagcttta
      361 caaaacatca tatgtgggaa atcttattca gataccaaga ttttgtcgcc atcaataaac
      421 ctcaaggcat atccgtccac cgttccggcg gcgaagtcag tctgacggca acacttgcgg
      481 cacagttggg cgtagaaaag gtatggttgc tgcacaggtt ggacaagcag gccggaggca
      541 tattgctgtt tgcccttaat ccccaaagtg ccgctgttct tgccgcacag tttgccgaaa
      601 ggaaaatgaa gaaaacctat ttggctttgt ccgaccgcaa accgtccaaa aaacaaggat
      661 ggataaaagg cggcatggaa aaatccaggc gcgggatgtg gaagctgacg cgcaatatgg
      721 aaaatattgc cgttacccgg tttttcagta tccgcatcag tgaaaaaatg cggctgttca
      781 tccttgagcc acatacgggt aagacacatc agttgagggt ggtaatgaag agtctgggca
      841 gctctatatt tggggacagc ctgtatggag gaacagaatc cgaaactatg ttcctgtatg
      901 cgtgaaaaat acaatttacc tatcaacaac aagaaatcga aattaccgca cctttgaaaa
      961 atgcatggca ggccgaaaat atctctcatg cactggaaga attttatatg gcaaaagcag
     1021 gttaatagat ataaatcaat taaaacaata tgataaatat ttttataaaa ttttttcaaa
     1081 aaacggttga ccgcaaatct ctttaagcgt atagttcagt tcgtccg
//
LOCUS       DAXFWM010000146         1119 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000146 DAXFWM010000000
VERSION     DAXFWM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.147"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(939..>1119)
                     /locus_tag="ACFI8B_002231"
     CDS             complement(939..>1119)
                     /locus_tag="ACFI8B_002231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835330.1"
                     /translation="FFDFILGISLIHAKIQPNVEKKITANTTLPPLTSNQTDSRFGWK
                     LVYLLFNGECQCSYP"
BASE COUNT          348 a          166 c          258 g          347 t
ORIGIN      
        1 gtactaaggg aaaaaattat aaacaatact gtttccagtg atgaaaaatt attgattatt
       61 aatgattttt tatcaggttt tcttgagttt gatcctgaga caggggaacc tgtcggggag
      121 acgcttaaaa ttgagcaaat gattgatttc tttttagtct aaatcataca taccagtgga
      181 caagctaggc gtagatccgg cttttaaata cgattgtcag gtttatactt gggataaaat
      241 caaaaataaa gttgtttaaa ctatagtgga ttaacaaaaa tcaggacaag gcaacgaagc
      301 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagggaatc
      361 gttctctttg agctaaggcg aggcaacgcc gtaccggttt ttgttaatcc actatattgc
      421 atggtgcatg cattaaatca gtcttctagc gccgcaatgg gaaaacatag aagataaacg
      481 aaatggatga tctaatacta tattttttat caggtatatt cggaaatcaa gttgctgaat
      541 atattataaa aaacaaccgc gagataaaag ttccctttat tgcgctttac gctatatttt
      601 ttacgttgat ttatactgtg gctttgttgt ttctcagtct gatctattgg gtcaatggtg
      661 cggagattgc atggaagggg ataggtattt tcagtatgtc ggtcagtttt tgtatagtct
      721 tctgtcttta tttgattgac aaggcaggaa gatgtaagga taagaaacaa tagggatcag
      781 atgcggcagc aaacgtagat tgggtctagg ctcaacctgc gtttgctgca tctttggaag
      841 aaatagtgga ttcgaaaaaa taggaagaat tctgattaat ggtatgcgag gtgtagcagt
      901 gggggctgtt gctggtggca taactggtta tgcaggttct acgggtaaga acactgacat
      961 tcgccgttaa ataataaata aaccaacttc caaccaaacc tgctatcggt ttggttggaa
     1021 gtgagaggag gtaaggtagt gtttgctgtt attttctttt cgacattggg ctgtatttta
     1081 gcatggataa gagatattcc taaaataaaa tcaaaaaaa
//
LOCUS       DAXFWM010000147         1116 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000147 DAXFWM010000000
VERSION     DAXFWM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.148"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..265)
                     /locus_tag="ACFI8B_002232"
     CDS             complement(<1..265)
                     /locus_tag="ACFI8B_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835331.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
     gene            complement(302..565)
                     /locus_tag="ACFI8B_002233"
     CDS             complement(302..565)
                     /locus_tag="ACFI8B_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835332.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(585..833)
                     /locus_tag="ACFI8B_002234"
     CDS             complement(585..833)
                     /locus_tag="ACFI8B_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835333.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            complement(794..>1116)
                     /locus_tag="ACFI8B_002235"
     CDS             complement(794..>1116)
                     /locus_tag="ACFI8B_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFC3835334.1"
                     /translation="SARWQDIDLTARLWHIPAADMKTKHPFTVPLSDWAVEILRELHT
                     QTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTPANPNTPCTDSATSS
                     PTSA"
BASE COUNT          170 a          232 c          401 g          313 t
ORIGIN      
        1 tttttttgac tgcccggcgg caggtttcgg taaggacggc ggcaaatcgg gcttcgtctg
       61 ccggtttgcc gccgaaaaaa ataaaatcgt aaagggtgat gccgttttcg gaatggcggt
      121 aatgcccgcc cgggcattcg ccgtctgtct gcctgtggat tttggcaatc aggcggggat
      181 agcggcaatg ggtaatgaaa cggcttgcgc cgtcttgccc gataatccat tcggggtagc
      241 ggttgaatag tgcggctctg ttcattttgt tgagggataa agcccctcgc ggggcttgtg
      301 gtcaggcaaa tttgaatatc agtgccaaca cggcggcgat ggcggtaacc agaccggtgg
      361 ccgccatcat gggataccag cgggactctt gggctatttt tacggattcg gcgttgattt
      421 tgtgcgcgtc ggcaatgatt ttggcgattt cggcatctat ttttcttaac tcggtgcgtt
      481 ttgtttcttg ttcgattatg tttattggat ttcctttcgt ttttggcggt tgccgccgct
      541 tgtcggatgg caggatgtct gccatgtatg tatgttaata ccttttaggt tttattgcaa
      601 gtgttttggg cggcggcttc gtatgcttgg cggtggcggc ggccgtacca ttcggcgagt
      661 tcgcgccgct cttggaggcg gtagctgtcg gcggtcaggt agtggtgcag gcttgagaag
      721 ccggcgcgtt ggagggcggc gcggctgatg tggccaaggg cgaggtctgt tgtgagggtg
      781 tcttttccgg cggtcaggct gatgttggtg aagaggtcgc ggaatccgtg catggtgtgt
      841 ttggatttgc cggggtgctg ccgtcatagc ccagtcgtcg gatggcgttg tgggcgaatt
      901 tgatgctgat gtggtcggga tggggggcgg gtttgcgccg tgggcggatg cctgggaaaa
      961 ggtggatgtt gtcgccggtc tgtgtgtgca gctctcggag tatttctact gcccagtccg
     1021 acagtgggac ggtaaagggg tgtttggtct tcatgtcggc ggcggggatg tgccataacc
     1081 gggcggtgag gtcgatgtct tgccagcggg cggaaa
//
LOCUS       DAXFWM010000148         1099 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000148 DAXFWM010000000
VERSION     DAXFWM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.149"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(89..412)
                     /locus_tag="ACFI8B_002236"
     CDS             complement(89..412)
                     /locus_tag="ACFI8B_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835335.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(415..603)
                     /locus_tag="ACFI8B_002237"
     CDS             complement(415..603)
                     /locus_tag="ACFI8B_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFC3835336.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(671..1009)
                     /locus_tag="ACFI8B_002238"
                     /pseudo
     CDS             complement(671..1009)
                     /locus_tag="ACFI8B_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          272 a          214 c          186 g          427 t
ORIGIN      
        1 tccagtcatt ctggctattt tatgtatatt tccattatgt ttgattatca ttttattttt
       61 tccaaggatt ttcttctata ttgatagttt atctaaagat ttcatttaaa atatcctgac
      121 taatttcttg tttatataat tctacaattt tctgtttctg tgtatttgta taaaattttt
      181 cgttggtatc tgcttgatat tttaattgtc tcaatatttc catgatttca tattctgatt
      241 tttcatcatg ttcttcaatc atgtaatcaa caaattgttt tagaatatca aaggcttcgt
      301 aactttccaa aattttaata tgtttatcgg caaaattgga catataccat tcgtcagtga
      361 ttggattggt atttaaatca tctaaaaaaa catttacatc atctaaaaac atggctaatc
      421 cttaaacttg aaatctgttt tctcaccggt tcttgtattt tcaatgtaat gtatctcaac
      481 tttgtttata ttttgaacgc gcttaaccca gccgtctttt gccaaccaac catttttagt
      541 atcggacata ggaggtattc tcgcaggagt tttgccctgt gggtttgcct taacttcttc
      601 cattgctaat tgttccttta aattattagg tattgtacga ccggttgatt tttcaacaga
      661 ttgtgtccct atttttaatg atttgtttcc ccccagccat ttccctgtct acagtctgca
      721 tatggcgggc agtaggtttt tcatagccgg tatctttaac tcaatcccat ttagtcgggt
      781 taagttctac ttttttacct ctccaaaccg tacctgcggc ctctgctacg gcaagggcag
      841 tttgggcagt agctgtaatg ttcggatggg catcagccca ttgtctggca gagttgatac
      901 cgtcttttac cgcaaaagca ctgtcctgta ataggctcac ggcggcaagt tgtgcttccg
      961 gacttaattt tcctgaatta ttaatacctt gtagagtctg ctgcgcagag atgtagcaac
     1021 tgtattttta ccccgtcggg taaaaataca gttgctacgg ctgctgggaa agcagttact
     1081 attttcccag tggctcaaa
//
LOCUS       DAXFWM010000149         1053 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000149 DAXFWM010000000
VERSION     DAXFWM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.150"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..>1053
                     /gene="tuf"
                     /locus_tag="ACFI8B_002239"
     CDS             <1..>1053
                     /gene="tuf"
                     /locus_tag="ACFI8B_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC3835337.1"
                     /translation="KKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVD
                     CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCD
                     MVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALD
                     SYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTT
                     CTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLS
                     KEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEE
                     GLRFAIREGGRTVGAGV"
BASE COUNT          276 a          296 c          275 g          206 t
ORIGIN      
        1 aaaaaattcg gcggcgctgc aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa
       61 gcacgcggta ttaccattaa cacctcgcac gtagaatacg aaaccgaaac ccgccactac
      121 gcacacgtag actgtccggg tcacgccgac tacgttaaaa acatgattac cggcgccgca
      181 caaatggacg gtgcaatcct ggtatgttct gctgccgacg gccctatgcc gcaaacccgc
      241 gaacacatcc tgctggcccg tcaagtaggc gtaccttaca tcatcgtgtt catgaacaaa
      301 tgcgacatgg tcgacgatgc cgagctgttg gaactggttg aaatggaaat ccgcgacctg
      361 ctgtccagct acgacttccc cggcgacgac tgcccgatcg tacaaggttc cgcactgaaa
      421 gccttggaag gcgatgccgc ttacgaagaa aaaatcttcg aactggctac cgcattggac
      481 agctacatcc cgactcccga gcgtgccgtg gacaaaccat tcctgctgcc tatcgaagac
      541 gtgttctcca tttccggccg cggtaccgta gtcaccggcc gtgtagagcg aggtatcatc
      601 cacgttggtg acgagattga aatcgtcggt ctgaaagaaa cccaaaaaac cacctgtacc
      661 ggcgttgaaa tgttccgcaa actgctggac gaaggtcagg cgggcgacaa cgtaggcgta
      721 ttgctgcgcg gtaccaaacg tgaagacgta gaacgcggtc aggtattggc caaaccgggt
      781 actatcactc ctcacaccaa gttcaaagca gaagtgtacg tattgagcaa agaagagggc
      841 ggccgccata ccccgttttt cgccaactac cgtccccaat tctacttccg taccactgac
      901 gtaaccggcg cggttacttt ggaaaaaggt gtggaaatgg taatgccggg tgagaacgta
      961 accattactg tagaactgat tgcgcctatc gctatggaag aaggtctgcg ctttgcgatt
     1021 cgcgaaggcg gccgtaccgt gggtgccggc gtg
//
LOCUS       DAXFWM010000150         1043 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000150 DAXFWM010000000
VERSION     DAXFWM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.151"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..978
                     /locus_tag="ACFI8B_002240"
     CDS             <1..978
                     /locus_tag="ACFI8B_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC3835338.1"
                     /translation="ARDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDR
                     ADEANRKMFEHNAKLDRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDK
                     AAMRNIAPLPAEGKFAAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPF
                     AKVKNLTKAAKPGKAAVSGDFSISYKNFSTVKPKVIAKGTINGKTFRDVNQSAKIGSP
                     DSPTLIAQRVNAKIQADGKPRPNATVANSHAEIGVIQQAYNAGETKGASMTMTVSGKD
                     VCGYCKGDIAAAAQASGLKSLTVNATDNVTGKNKTYYWTPGMKSIKERK"
BASE COUNT          326 a          258 c          245 g          214 t
ORIGIN      
        1 gcacgcgacg aatacaccta tcacgtcaac ggcacagccc gcagtatcaa actcaatccg
       61 accgacaccc gcagcatccg gcaacgcata tccgacaact acaacaacct cggcagcaat
      121 ttctccgacc gcgccgatga agccaacaga aaaatgttcg agcacaatgc caagctcgac
      181 cgctggggca acagcatgga gtttatcaac ggcgtcgccg ccggcgcgct caaccccttt
      241 atcagcgcgg gcgaagcctt gggcataggc gacatactgt acggaacgcg ctatgccata
      301 gacaaagccg caatgcgcaa catcgccccc ttacccgccg agggcaaatt cgccgccatc
      361 ggcggcttgg gcagcgtggc gggctttgaa aagaatacgc gtgaagccgt tgaccggtgg
      421 atacaggaaa accccaatgc cgccgaaacc gtcgaagccc tggtcaacgt cctgccgttt
      481 gccaaagtca aaaacctgac aaaggcggcg aaaccgggga aagctgcggt tagcggggat
      541 ttttcgatta gctataaaaa cttttctact gtcaaaccaa aagtaattgc caaaggaacg
      601 attaatggaa agacttttag agatgttaat caaagtgcaa aaattggatc gcctgattca
      661 cctacattaa tagcccaacg agttaatgca aaaatccaag ccgacggtaa acctcgtccg
      721 aatgcaaccg ttgctaattc acatgctgaa attggtgtta tccagcaggc ttataatgca
      781 ggtgaaacga aaggggcatc tatgacaatg acagtttcag gaaaagatgt ttgcggttat
      841 tgtaaaggag atattgcagc tgctgctcaa gcttctggac tgaaatcact gactgttaat
      901 gcgacagata atgtaacagg aaaaaataaa acttactatt ggacgcctgg tatgaaatca
      961 attaaggaga gaaaatgaaa acatcaacaa ttgtttttgg tggatttttt attacagata
     1021 atggagaacg aatccaaatc cct
//
LOCUS       DAXFWM010000151         1023 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000151 DAXFWM010000000
VERSION     DAXFWM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.152"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..317
                     /locus_tag="ACFI8B_002241"
     CDS             <1..317
                     /locus_tag="ACFI8B_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3835339.1"
                     /translation="ATDIKGKYVKEVKVANGVVTATMNSTGVNKEIKGKRLSLWGRRE
                     NGSVKWFCGQPVTRAKADADADAAGKDTTNIDTKHLPSTCRDAASAVCIETPPTAFYK
                     NT"
     gene            413..838
                     /locus_tag="ACFI8B_002242"
     CDS             413..838
                     /locus_tag="ACFI8B_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3835340.1"
                     /translation="MPTDPDSRLRGNDEAILLAEGQKSAVTEYYLNNGIWPENNGDAG
                     VASASEIKGKYVKSVTVTNGVVTAQMNPSGVNNEIKGKKLSLWAKRENGSVKWFCGQP
                     VTRTGDNDDTVADAKDGNGNEKIETKHLPSTCRDPFSAS"
BASE COUNT          300 a          297 c          244 g          182 t
ORIGIN      
        1 ccgccaccga catcaaaggc aaatatgtta aggaagttaa agtcgcaaac ggcgtcgtta
       61 ccgccacaat gaattcaacc ggcgtaaaca aagaaatcaa aggcaaaaga ctctccctgt
      121 ggggcaggcg tgaaaacggt tcggtaaaat ggttctgcgg acagccggtt acgcgcgcca
      181 aagccgacgc cgacgccgac gccgccggca aagacaccac caacatcgac accaagcacc
      241 tgccgtcaac ctgccgcgac gcagcatctg ccgtttgcat agaaacacca cctacggctt
      301 tctataaaaa tacctaaacc gtcattccca cgaacctaca tcccgtcatt cccacgaaag
      361 tgggaatccg gaacgcaaaa tctaaagaaa ccgttttacc cgataagttt ccgtgccgac
      421 agacccggat tcccgcctgc gcgggaatga cgaagccatc cttttggccg aaggtcaaaa
      481 atcagccgtc accgagtatt acctgaataa cggcatatgg ccggaaaaca acggcgatgc
      541 cggcgtggca tccgcttcag aaatcaaagg caaatatgtt aaaagcgtta cggtcacaaa
      601 cggcgtcgtt accgcccaaa tgaatccaag cggcgtaaac aatgaaatca aaggcaaaaa
      661 actctccctg tgggccaagc gtgaaaacgg ttcggtaaaa tggttctgcg gacagccggt
      721 tacgcgcacc ggcgacaacg acgacaccgt tgccgacgcc aaagacggca acggcaacga
      781 aaaaatcgaa accaagcacc tgccgtcaac ctgccgtgac ccgttctctg ctagctaagg
      841 caaattaggc cttaaatttc aaacaaatca aacggtaagt gatttcccac ggccgcccgg
      901 atcaacccgg gcggcttgtc ttttaagggt ttgcaaggcg ggcggggtcg tccgttccgg
      961 tggaaataat atatcgattg cgcttcaagg ccctgcatgt gcctcattgc cacccgttta
     1021 aac
//
LOCUS       DAXFWM010000152          999 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000152 DAXFWM010000000
VERSION     DAXFWM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.153"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..183
                     /locus_tag="ACFI8B_002243"
     CDS             <1..183
                     /locus_tag="ACFI8B_002243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835341.1"
                     /translation="AKPQTWRLLYAVRKPFRRHFSLILLGEWLTLNVSTFIIQFIGAS
                     GADTGKAPPKRGLYRE"
     gene            338..505
                     /locus_tag="ACFI8B_002244"
     CDS             338..505
                     /locus_tag="ACFI8B_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835342.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            665..>999
                     /locus_tag="ACFI8B_002245"
     CDS             665..>999
                     /locus_tag="ACFI8B_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3835343.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGCQVGP"
BASE COUNT          301 a          232 c          227 g          239 t
ORIGIN      
        1 gccaagccgc aaacttggcg gcttctttat gccgtccgaa agcccttcag acggcatttt
       61 tctttgattt tgttggggga atggttgact ttgaacgtgt ccacgtttat aattcaattc
      121 atcggcgcat cgggtgccga tacgggaaaa gccccaccga agcggggctt gtacagggag
      181 taggaaaatg aagctcatcc ttcaaattct catcctggtt ttaatcttga tgctggcagg
      241 cgatacgcct attagcggat aaaaccggct ggggcggtag ccgccgcccc agtccctatc
      301 ttacaaaagg cagttcaaaa aatcaaggag tttgcctatg gttaatgaaa aactggcaga
      361 aaaccgaaag cgttacgagc aaaaacgagt cattaaaaaa gtctcgttta atgcggaaac
      421 agaaaaagag cttttggaat atgcccaaaa tctcgatttt tcccaatggg ttaaatcaat
      481 cataaaagaa aaaatcaaaa agtaattgac tttgaacgtg tccacgttta ttattcttac
      541 aacggcacag ccgttaatgg aaaaccgaac caagcgggaa cttggttcgg ttaagtgaaa
      601 agcagttcta ggaaaactct tttcgcgtct tttaaataac ctctaaaaga aagactattc
      661 tatcatgaat gctgtccaag ttttaaactt tcaacaaaac tccgtccgta ctgttgccga
      721 taacaaaggc gagttgtggt ttttggcaaa cgatgtttgc gagattttag gctacaccaa
      781 ccctcgccga acggttgacc tgcactgcaa aagcaggggc gtaacaaaac ggtacacccc
      841 caccgccagc ggcgaacagg agatgaccta tattaacgag ccgaatctct accgcttgat
      901 tatcaaatcc cgcaagcctg ccgcagaagc gtttgaagaa tgggtaatgg aaaccgtcct
      961 gcccgccatc cgcaaaacag gcggctgcca agtcggacc
//
LOCUS       DAXFWM010000153          996 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000153 DAXFWM010000000
VERSION     DAXFWM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.154"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..594
                     /gene="mafB"
                     /locus_tag="ACFI8B_002246"
     CDS             <1..594
                     /gene="mafB"
                     /locus_tag="ACFI8B_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3835344.1"
                     /translation="AGTVWGGKKVELNPAKWDWVKNTGYETTVTRKTLDGQIAGKNKP
                     IKPSSGDYVLPVGRQIIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRV
                     DVVNMPDGAPTSMDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQTWG
                     EAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            591..962
                     /locus_tag="ACFI8B_002247"
     CDS             591..962
                     /locus_tag="ACFI8B_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC3835345.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          345 a          161 c          213 g          277 t
ORIGIN      
        1 gcaggtacgg tttggggcgg taaaaaagta gaacttaacc cggccaaatg ggattgggtt
       61 aaaaataccg gctatgaaac aactgttacc cgaaagactt tagatgggca aatagctggc
      121 aagaacaagc ctattaaacc ttcttcagga gattatgtct tgccggtagg cagacaaatc
      181 atagatccta ctcgtacatc tttttcacaa gctacagtta gctatcagaa gcgtggtgca
      241 aattacaact atgattctct tgtagctgca atgaacgaaa aaaaatcatg ggttggcgac
      301 agggttgatg ttgttaatat gcctgatgga gcacctacta gtatggataa cacgcgtatt
      361 atggcagcac gtgaagcagg ggtaaaagtg gaagcgaatg ttcataattt taatgaccga
      421 ttatcatcaa aagagagaat caggtttaag catgatggta ttgagcctca aacttgggga
      481 gaagctatcc agctacgaat tagaaagcaa gaaacacaaa aaggagttcc agaagggtgg
      541 agcaaaagat ttcctaacgg aagtatttat gatgtaaagg tacttaggaa atgataaaac
      601 aaaatagttt tgttccgtat cctgaagcaa tgcttcctaa aggatttaaa tatccgcaaa
      661 gttatttaaa attagctcaa tccactcatg ccattaacta cgatgaacaa tattcttttc
      721 cttggtggtt tgaaaatgca gaaagcaata tatcagaagt aattgacatt tattttgaaa
      781 taactggcat tccaaaccta ttaccttttg ctagaaacca agagtgggct gcctgttttg
      841 atatttcaga taaatcaggt aatcctaaaa ttatagtagt taatttagat aatacaaaat
      901 attacgagac ttttgaaaat tttgatactt ggctaaaaga agctgaaaat gatggttggt
      961 agcagccgta ggtcggattc tcgaatccga catttt
//
LOCUS       DAXFWM010000154          991 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000154 DAXFWM010000000
VERSION     DAXFWM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.155"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..246)
                     /locus_tag="ACFI8B_002248"
     CDS             complement(<1..246)
                     /locus_tag="ACFI8B_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835346.1"
                     /translation="MRTHIRTCVYHDSGTKGSNTASGISGTAAGAAEQAFFSAAKTAN
                     RSASAASANAPPHPDKPKNGRKPMTFQGHNNRKKAGGY"
     gene            complement(243..>991)
                     /locus_tag="ACFI8B_002249"
     CDS             complement(243..>991)
                     /locus_tag="ACFI8B_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835347.1"
                     /translation="LQDMRYACNQELINFAEHIVKQVQRLGLYCNTDDPENEESVLFA
                     CREASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLA
                     VRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFTGRILHAANLLYDV
                     GIQADKELSAMYGRPLNPVRPRRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCD
                     NGAGFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT          163 a          288 c          295 g          245 t
ORIGIN      
        1 gtagccgccg gcttttttac ggttgttgtg tccttgaaac gtcatgggtt ttcgtccgtt
       61 ctttggcttg tccgggtgtg gcggagcgtt tgcggatgcg gctgacgcgc tgcggtttgc
      121 cgtcttcgcc gcgctgaaaa acgcttgttc cgccgcgccg gcagccgtgc cgcttatgcc
      181 tgatgccgtg tttgagcctt ttgtgccgga atcgtgatac acgcaggttc ggatgtgcgt
      241 cctcatgccg ccgcttccct gtgcatgagg ctgatcaggt ttgccgtccg tttgaaatgt
      301 tcggtccagt tgaagcagct gaagccggcg ccgttgtcgc aatgccggat gacgtcttcc
      361 gagtctttta cggcccggac caacgcgccg cctttgtccg caacaagcat tttcatcatc
      421 ggccggcgca cgtcgcttat ccgtcggggg cgcacgggat tcagcggcct gccgtacatc
      481 gccgaaagct ccttgtccgc ctgtatgccc acatcgtaaa gcaggtttgc ggcgtgtagg
      541 atccggcctg taaattccgc gtacggcatt ttcaggcggc gggcttccgc gcgcgaatcc
      601 gcgccgttga cgactttgcc gaccgccgaa atcaaaccgc cgtcaagttc taaaaacgcc
      661 atggctttct tggcaacccg cacggccaat gagtatgccg atacttcggc tatcagccgt
      721 gcaggcgcgt ctttcagaaa ttcttcgtac gcggcgaaga gatttgacag cggacgcagc
      781 acgagttggc gctgattcgg gctaaggtcg tcgaaatcct tagtccattg cgcgacggcc
      841 tgcgatgctt cgcggcaggc aaacagtacg ctttcctcgt tctcggggtc gtccgtgttg
      901 cagtacaggc ccaagcgttg cacctgtttg acgatatgct cggcgaagtt gattaactcc
      961 tgattgcagg cgtaacgcat atcctgcagg g
//
LOCUS       DAXFWM010000155          954 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000155 DAXFWM010000000
VERSION     DAXFWM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.156"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..220)
                     /locus_tag="ACFI8B_002250"
     CDS             complement(<1..220)
                     /locus_tag="ACFI8B_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3835348.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRL"
     gene            complement(211..594)
                     /locus_tag="ACFI8B_002251"
     CDS             complement(211..594)
                     /locus_tag="ACFI8B_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFC3835349.1"
                     /translation="MNEAKFTLTPENKKEVMRLIWDNLNGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(591..896)
                     /locus_tag="ACFI8B_002252"
     CDS             complement(591..896)
                     /locus_tag="ACFI8B_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFC3835350.1"
                     /translation="MSSAIRKAAKGEQCTLNIAGVCNDNPETTVLCHFPSETHGMGLK
                     SDDLSAGFGCSCCHDAIDGRSGHLSREDKEFYMRRSQFRTIRRLEALGIVSVKGRLK"
BASE COUNT          227 a          261 c          168 g          298 t
ORIGIN      
        1 ttaatctatg tgcattatat tgtttcttaa atatttttaa cttcaaatac cccaacctgc
       61 ctttgtataa gaaaacctcc ttgccggaaa atccggtatt ccaagatttg taatgcaaac
      121 cgctattgaa atactttctt ggacgctccg cccaatataa ctttcccgta ttgtcatcat
      181 atcttaataa ttcttttaaa acctcttgtg ttaatcccat aaaacaccat gctccataga
      241 tgcccaagat ataatctttt cttgatattc agacatttcc cgaactgata attttgtcgt
      301 tgatataccc attaattgcc cgttaggcat ttcaatacat ccaataaatt ttctttttaa
      361 aaattcatgc catacatctt gactaaacct tctcccatcg ataaaaactt tttctgaaat
      421 ttcacgatac aaaaaccata gccgcctgtt ctgctcgacg ctccgttttg acttgcgcgg
      481 gcggattgtg atttcaagat tgccgttttc aaaccacccg ttcaggttgt cccaaatcag
      541 gcgcatgact tcctttttgt tttcgggcgt cagcgtgaat tttgcttcat tcatttcaaa
      601 cggcctttca cgctgacaat ccccaatgct tcaaggcggc gtatcgtgcg gaattgggag
      661 cggcgcatat aaaactcttt gtcttcgcgg ctcaaatgcc cgcttctgcc gtcgatcgcg
      721 tcgtggcagc aactgcaccc gaaacccgcg ctcaagtcgt cgcttttcaa ccccatcccg
      781 tgcgtttcgc tggggaagtg gcacaacacg gttgtttcag ggttgtcatt gcacacgccc
      841 gcgatattca gcgtgcattg ctcgcctttg gcggctttgc gtatcgcgct agacatccca
      901 accctcctgt ccgatgaaat cccaaccctc gcctgccgca tccgtctgtt ccat
//
LOCUS       DAXFWM010000156          920 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000156 DAXFWM010000000
VERSION     DAXFWM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.157"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            162..515
                     /locus_tag="ACFI8B_002253"
                     /pseudo
     CDS             162..515
                     /locus_tag="ACFI8B_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            513..773
                     /locus_tag="ACFI8B_002254"
     CDS             513..773
                     /locus_tag="ACFI8B_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3835351.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
BASE COUNT          325 a          169 c          189 g          237 t
ORIGIN      
        1 atcaacaaag caatgcatta tttatctcaa ataaaattag agaatgcata cgtgtctttt
       61 gttttgaaat ttatctaaca aaggaagcac aagaataggt ttatagtaaa acatcaagat
      121 gttgaaaatg ctaggtttta acctaaccta cgctgaccgg atcagataca gccgctcagc
      181 agactctaca aggtattaat gatttaggaa atttaagtcc ggaagcacaa cttgccgccg
      241 cgagcctatt acaggacagt gcctttgcgg taaaagacgg catcaattcc gccagacaat
      301 gggctgatgc ccatccgaat ataactgcaa cagcccaaac tgcccttgcc gtagcagagg
      361 ccgcaggtac ggtttggaga ggtaaaaaag tagaacttaa cccgactaaa tgggattggg
      421 ttaaaaatac cggctataaa aaacctgctg cccgccatat gcagacttta gatggggaga
      481 tggcaggagg aaacaagcca gttgttaaat ctattagacc aactacgcga gatgaattac
      541 gtcaagcatt gcaagaacaa ggttttagac gtactggttc agatgcggct caatatgaaa
      601 catggaaagg tcctgatggc gtgaaaatag atattcgtcc aaatggagag gttataagaa
      661 cccaaagagt gccgcgaacc gatggtgtac agggaaaata tccgcaacga caagattatg
      721 aaggcaatcc attgccaaat aatcatcatc attctggata ttttgtcaaa tgaaaaaaaa
      781 tatttttcac aatgtaagcc tttatgaaat aatcttttcc gataatggaa atacccttac
      841 attatctttt acagatacaa ttgaaggtaa ttatttcgga tatatcaaat gcagtaatat
      901 tttgaatttt aaattagata
//
LOCUS       DAXFWM010000157          839 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000157 DAXFWM010000000
VERSION     DAXFWM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.158"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            315..641
                     /locus_tag="ACFI8B_002255"
                     /pseudo
     CDS             315..641
                     /locus_tag="ACFI8B_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          236 a          208 c          212 g          183 t
ORIGIN      
        1 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagcca
       61 caaaaataaa atagggaggg atgtcgtgat atttgaaaaa tcaataaacg gctggaaata
      121 ccgcgccgta atggaaatca gaaccacgca aaaggagctt gccttgaaca gcctttatac
      181 aacagacagg attgaatagt catgacaaaa cctgcgacga gtaactgtca atctttcgat
      241 tccgtcctca tacatcctaa gcgtctttgc aaattctgtt cagttatacc atttatacag
      301 acaggagcaa accaatgacg aagttatacg cagaaatcgc cgaaatggag gcgcaggacg
      361 acggcacggt caaagtttgg ggttatgcct caagcgaggc ggtcgattcg gacggcgaaa
      421 tcatcgcggc ggcggctatg aaggcggcga ttcccgatta tatgaagttc ggcgaggtgc
      481 tcgagatgca cggcagcaac gcggcgggaa cggcgattga aatcaacgtg gaagacgacg
      541 gcagaacctt tttcggggcg catatcgtcg atcccgttgc agtggagaaa gtcaaaacgg
      601 gcgtttacaa gggcttttcc atcggcggca gcgttaccgc tgtctgctta ggttgaatcg
      661 gcattcgtac gattacggca caccgcctaa tctgtacctg cgtctgttgc gcccaatacc
      721 aaaggctacc tgaaaatctc ccaaactttt caggtagcct tatcacgttt tcaactgccg
      781 ccacacgccc aaagaccgcc tgaaaccctt tttcagacgt tccgctctga tacacccaa
//
LOCUS       DAXFWM010000158          812 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000158 DAXFWM010000000
VERSION     DAXFWM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.159"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..683
                     /locus_tag="ACFI8B_002256"
     CDS             <1..683
                     /locus_tag="ACFI8B_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3835352.1"
                     /translation="MGAWLEVLSEEKDGNRLARHHKHGEIWKKPTRHEDIAAVFPFGN
                     PIHNNTMIMRRSVIDGGLRYDTGRDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQ
                     VSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELPEKDLPEED
                     FERARRFLYRCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILYRLIKNRRQARSD
                     SAGKEQGI"
     gene            683..>812
                     /locus_tag="ACFI8B_002257"
     CDS             683..>812
                     /locus_tag="ACFI8B_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3835353.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLER"
BASE COUNT          202 a          218 c          231 g          161 t
ORIGIN      
        1 cgatgggcgc gtggctggaa gttttgtcgg aagaaaagga cggcaaccgg ctggcgcggc
       61 accacaaaca cggcgaaatt tggaaaaagc cgacccggca tgaagacatc gccgccgttt
      121 tccctttcgg caaccccata cacaacaaca cgatgattat gcggcgcagc gtcattgacg
      181 gcggtttgcg ttacgacacc gggcgggatt gggcggaaga ttaccaattt tggtacgatg
      241 tcagcaaatt gggcaggctg gcttattatc ccgaagcctt ggtcaaatac cgccttcacg
      301 ccaatcaggt ttcatccaaa cacagcgtcc gccaacacga aatcgcgcaa ggcatccaaa
      361 aaaccgccag aaacgatttt ttgcagtcta tgggttttaa aacccggttc gacagcctag
      421 aataccgcca aacaaaagca gcggcgtacg aattgccgga gaaggatttg ccggaagaag
      481 attttgaacg cgcccgccgg tttttgtacc gatgcttcaa acggacggac acgccgccct
      541 ccggcgcgtg gctggatttc gcggcagacg gcaggatgag gcggctgttt accttgaggc
      601 aatacttcgg cattttgtac cggctgatta aaaaccgccg gcaggcgcgg tcggattcgg
      661 cagggaaaga acaggggatt taatgcaaaa ccacgttatc agcttggctt ccgccgcaga
      721 acgcagggcg cacattgccg ataccttcgg cagtcgcggc atcccgtttc agtttttcga
      781 cgcactgatg ccgtctgaaa ggctggaacg gg
//
LOCUS       DAXFWM010000159          731 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000159 DAXFWM010000000
VERSION     DAXFWM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.160"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(85..>731)
                     /locus_tag="ACFI8B_002258"
     CDS             complement(85..>731)
                     /locus_tag="ACFI8B_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISNgo2 family transposase"
                     /protein_id="HFC3835354.1"
                     /translation="YCRSAQESELVKRQKPTDEQYRLLRMTAAYAQIKSECAAMKNRH
                     HAAKDEEAAKAYAEIIKAMNEQLEVLKEKIKEQTEKPNCKEGVKRLETIPAIGRMTAA
                     VLFHHLTSSKFETSNKFAAFAGLSPQQKESGTSVRGKGKLTKFGNRKLRAVLFMPAMV
                     AYRIRAFPDFIKRLEEKKKPKKVIIAALMRKLAVIAYHVHKKGGDYDPSRYKSA"
BASE COUNT          143 a          179 c          156 g          253 t
ORIGIN      
        1 attgaatttc aatatttgaa aattcaccgg atttcagacg cggcaaagca ggcatttaaa
       61 aaatgccttt tttcctttcg ggatttacgc cgatttgtaa cgcgatggat cgtaatctcc
      121 gcctttctta tgtacgtgat acgcaataac ggcgagttta cgcatcaatg ctgcgatgat
      181 gactttttta ggcttcttct tttcttccag ccttttgatg aagtcgggaa atgcccttat
      241 ccggtatgcg accatggccg gcataaacaa gacggcgcgt aatttcctgt tgccaaactt
      301 ggtcagtttg ccttttcccc ttacgcttgt cccggattct ttttgttgcg ggcttaagcc
      361 tgcgaacgct gcaaatttgt ttgatgtttc aaatttcgaa gatgttagat gatgaaacaa
      421 tacggctgcg gtcattctgc ctattgccgg tatggtttca agacgcttca cgccttcctt
      481 gcagttaggc ttctccgtct gctcttttat cttctccttt aaaacttcaa gctgttcatt
      541 catggctttg atgatttccg catatgcttt ggccgcttct tcatcttttg ccgcgtgatg
      601 acggtttttc attgccgcgc attcgctttt gatttgcgcg tatgctgcgg tcatccgtaa
      661 aagcctgtat tgctcgtccg taggcttctg cctctttaca agctcgcttt cctgcgccga
      721 ccggcaatac t
//
LOCUS       DAXFWM010000160          645 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000160 DAXFWM010000000
VERSION     DAXFWM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.161"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..497)
                     /locus_tag="ACFI8B_002259"
     CDS             complement(<1..497)
                     /locus_tag="ACFI8B_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835355.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRT"
     gene            complement(490..>645)
                     /locus_tag="ACFI8B_002260"
     CDS             complement(490..>645)
                     /locus_tag="ACFI8B_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3835356.1"
                     /translation="SQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSVR
                     CSGARDG"
BASE COUNT          241 a           97 c           99 g          208 t
ORIGIN      
        1 gttctaaatt cctgtttact aatcagcact aataataacc ctgattcagt ttttgcttga
       61 taaaatcgat agccaaataa gaaaaaaaca gggttaaaat aaaatgactt agagcatgaa
      121 actagtaaga aaataattcc atacataaca aatagcaaaa aaagattatt atcgggtatg
      181 cttagagcaa caaaaaaata accaagatag ctaggtaaga aattatcgtt aacatattct
      241 aattctttca cctctccttg cttgaattca tccttcccca agtatttgct tagccataag
      301 acagtagcgg ttgcgataaa tggtattaga aaataaaaaa gtagacttaa cttagaaata
      361 ggttctatcc aatgatttga aattacacga taaaaccaag taaggtctaa tatataatat
      421 tgcgatttaa taaaatatat tgataacagc aaagatgtac tacttatatt taataatatt
      481 ttaaatagtt tatccatctc ttgctccgct acatcttacg gatacctgcc cgttcatcag
      541 catgggtagt aggaaatcgc gtagttgggt caggtggtgg ttttgtttta gattatttcg
      601 aactttaaca aagaaagtat ttgctatttc attatatttt tgaga
//
LOCUS       DAXFWM010000161          566 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000161 DAXFWM010000000
VERSION     DAXFWM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.162"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            143..>566
                     /locus_tag="ACFI8B_002261"
     CDS             143..>566
                     /locus_tag="ACFI8B_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3835357.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNNGKWPADNGAAGVASSATDIKGKYVKEVKVANGVVTAQMNPS
                     GVNNEIKGKKLSLWAKRQDGSVKWFCGQPVTRAAADAKD"
BASE COUNT          160 a          157 c          129 g          120 t
ORIGIN      
        1 atttcacctc aaaacataaa atcggcacga atcttgcttt ataatacgca gttgtcgcaa
       61 caaaaaaccg atggttaaat acattgcatg atgccgatgg cgtaagcctg aggcatttcc
      121 cctttcaatt aggagtaatt ttatgaatac ccttcaaaaa ggctttaccc ttatcgagct
      181 gatgattgtg atcgccatcg tcggcatttt ggcggcagtc gcccttcccg cctaccaaga
      241 ctacaccgcc cgcgcgcaag tttccgaagc catccttttg gccgaaggtc aaaaatcagc
      301 cgttaccgag tattacctga ataacggcaa atggcccgcc gacaacggcg ctgccggcgt
      361 ggcatcctcc gccaccgaca tcaaaggcaa atatgttaag gaagttaaag tcgcaaacgg
      421 cgtcgttacc gcccaaatga atccaagcgg cgtaaacaat gaaatcaaag gcaaaaaact
      481 ctccctgtgg gccaagcgtc aagacggttc ggtaaaatgg ttctgcggac agccggttac
      541 gcgcgccgcc gccgacgcca aagacg
//
LOCUS       DAXFWM010000162          555 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000162 DAXFWM010000000
VERSION     DAXFWM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.163"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..217)
                     /locus_tag="ACFI8B_002262"
                     /pseudo
     CDS             complement(1..217)
                     /locus_tag="ACFI8B_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            complement(254..>555)
                     /locus_tag="ACFI8B_002263"
     CDS             complement(254..>555)
                     /locus_tag="ACFI8B_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835358.1"
                     /translation="TSTLMSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEF
                     HHDKVIFYHNEFDEEDGYPVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
BASE COUNT          173 a          111 c           77 g          194 t
ORIGIN      
        1 atatatttaa atgcttacca tccctactag cccatccaag ttgctctctt cctgttttag
       61 acaattgaac atcccattca aattcttgac ctttagttct tgatggacct ttagtccatt
      121 cattaccaaa tttatccaaa tatccattat taggtccttt tggtagcggt gctgaaggag
      181 agtaattttt aggtgggata tatctgattc tacctttcta tatcgctgat tctattcatt
      241 aagctaatta tgcttattcc tcaatcacag tctccaccac cgaatgaata cttttaggca
      301 attgcaaaaa agcattccaa gcaattaaag cagttttaaa agtatgcagc gaacagctta
      361 atactggata accatcttct tcatcaaatt cattgtgata aaaaatgacc ttatcatgat
      421 gaaattctag gcaaaattgt tgaccaccgc catcatcata aatttcattt ggattatttt
      481 ctattaattc tataatcttg aaatgctcat caatcgtgga tcgatagtaa atatcgctca
      541 ttaaggtaga cgtaa
//
LOCUS       DAXFWM010000163          548 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000163 DAXFWM010000000
VERSION     DAXFWM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.164"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(72..>548)
                     /gene="mafB"
                     /locus_tag="ACFI8B_002264"
     CDS             complement(72..>548)
                     /gene="mafB"
                     /locus_tag="ACFI8B_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3835359.1"
                     /translation="ITATAQTALAVAEAATTVWRGKKVELNPTKWDWVKNTGYKKPAA
                     RHMQTVDGEMAGGNKPLESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSVDAFS
                     ENGTVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQLPEK"
BASE COUNT          142 a          116 c           83 g          207 t
ORIGIN      
        1 attcctatca aagattcccc ttgtctcgaa taaatttcaa aaatttcatt taacatatta
       61 atctctatct actatttttc aggaagttgt tggttaggga caaataatct atgattaatt
      121 tttccatctg cttctctgat atattcaaaa tttccatctt ttcctttata agatccaggg
      181 atataaagtt tatgccttac aatattatct ccgccaacta tttgtattac tgttccattt
      241 tcagaaaaag catctactga ttgcggaaaa ccatgataat caccattcga tgcttgcctt
      301 aatactttct ctgtataacc ggtattttca aaaaaattat tcgttgttac agtatttttt
      361 gattccagcg gtttattccc accggccatt tccccgtcta cagtctgcat atggcgggca
      421 gcaggttttt tatagccggt atttttaacc caatcccatt tagtcgggtt aagttctact
      481 tttttacctc tccaaaccgt agttgcggcc tctgctacgg caagggcagt ttgggctgtt
      541 gctgttat
//
LOCUS       DAXFWM010000164          540 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000164 DAXFWM010000000
VERSION     DAXFWM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.165"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(43..>540)
                     /locus_tag="ACFI8B_002265"
     CDS             complement(43..>540)
                     /locus_tag="ACFI8B_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3835360.1"
                     /translation="AFVVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIIS
                     REAMRFFLDRFAVLPAERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQ
                     NSMLGSDLEKDREQGRRHRRSLKAMFDLKRALGKFGREKKKRMESQRQAELEKVYGRR
                     VILFK"
BASE COUNT          151 a          155 c           84 g          150 t
ORIGIN      
        1 gtgtgtccat ttctgatttt aatcccctat attttacaca aactatttga acaatatgac
       61 ccgcctgccg taaactttct caagctccgc ctgcctttga ctctccattc ttttcttctt
      121 ttccctaccg aatttaccca aagcacgctt caagtcaaac atcgccttca acgaacggcg
      181 gtgtcttctt ccttgttccc tatctttttc caaatcgcta cccaacatac tgttttgact
      241 gagaaacttg gcataatgca attcttgggt acataaggcg ggactaacct gataaacagg
      301 catcccctcc ttatcaaaga aataagtaaa catcatcaaa tctaccgctt taatccgctc
      361 tgccggcaaa acggcaaacc tgtccaagaa aaaccgcatc gcctcacgcg aaatgatata
      421 gccagccgtc ccacaatgtt cgctctccag caaaggaaat gaccggtttt cataattcag
      481 gactttatcc ggtctgacaa taactttcgc aaacatcgtt tccaagcgga cgacaaaggc
//
LOCUS       DAXFWM010000165          499 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000165 DAXFWM010000000
VERSION     DAXFWM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.166"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          163 a           92 c           95 g          149 t
ORIGIN      
        1 tatctttaaa tgtaaacatt tgtagattaa cgttatgccc atcatttcat gatgaacatg
       61 atacgcttgt ttgttcagtg caagattttt atgtaaatac tgtttaacta gcaagcgcag
      121 gctggattaa aacctaaatc attgataaaa aacataaaac aagcattctg aaatttttca
      181 ggctgctttt ttgttgggtt tggtaaccca atctactcgc tgaaaattag cagaggacgt
      241 aaatggctat ttatgattta aacgaaatcg taacccgtgc tgcattaggt atatataaag
      301 gaaaatggcg aaacatggat tttgagcaag ttctacaaaa cattgttcag caaccgtagg
      361 tcggattctc gaatccgaca tttttcaaca gcggcatttc ggaaacgata gacgcgtcaa
      421 atatttttgt cggatacaag tatccaacca tacggcaatc atacgggtaa ctccgcccca
      481 tccgtagagg ctgataaca
//
LOCUS       DAXFWM010000166          481 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000166 DAXFWM010000000
VERSION     DAXFWM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.167"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..>481
                     /locus_tag="ACFI8B_002266"
     CDS             <1..>481
                     /locus_tag="ACFI8B_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC3835361.1"
                     /translation="ATTVWGGKKVELNPAKWDWVKNTGYKKPAARHMQTVDGEMAGGN
                     RPPKSITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGKKNFPIGTATYE
                     EADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQANFETYTIDSN
                     "
BASE COUNT          175 a           81 c          116 g          109 t
ORIGIN      
        1 gcaactacgg tttggggagg taaaaaagta gaacttaacc cggccaaatg ggattgggtt
       61 aaaaataccg gctataaaaa acctgctgcc cgccatatgc agactgtaga tggggagatg
      121 gcagggggga atagaccgcc taaatctata acgtcggaag gaaaagctaa tgctgcaacc
      181 tatcctaagt tggttaatca gctaaatgag caaaacttaa ataacattgc ggctcaagat
      241 ccaagattga gtctagctat tcatgagggt aaaaaaaatt ttccaatagg aactgcaact
      301 tatgaagagg cagatagact aggtaaaatt tgggttggtg agggtgcaag acaaactagt
      361 ggaggcggat ggttaagtag agatggcact cgacaatatc ggccaccaac agaaaaaaaa
      421 tcacaatttg caactacagg tattcaagca aattttgaaa cttatactat tgattcaaat
      481 g
//
LOCUS       DAXFWM010000167          461 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000167 DAXFWM010000000
VERSION     DAXFWM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.168"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(248..>461)
                     /locus_tag="ACFI8B_002267"
     CDS             complement(248..>461)
                     /locus_tag="ACFI8B_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835362.1"
                     /translation="YPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTI
                     LVMGFSSKILFSSHNKIKEFNSNKYD"
BASE COUNT          155 a           79 c           72 g          155 t
ORIGIN      
        1 cctgctttat ttggataacc atgaggcatg gctaccttta tccggaaaaa tattaatagc
       61 cgcaacaaag atatttgaat ccgattcaaa tatcaccagc aagcgtagct cggatacttg
      121 tatccgacaa agcctttaac atttccatcg ttgcaatcca ttgcagcaat taccaaaatg
      181 tcggattcga gaatccgatc tacggaaaac taaagatttt atgcgggcta cggcttgctt
      241 gttgtattta atcatattta tttgaattga attcttttat tttattgtga gatgaaaata
      301 gtattttcga tgagaaaccc atgactaaaa tagtatagtc tttatcattc tttacgaagt
      361 aacaattacc atatcctgca atatttttat cccaaaaatc acaatatgat tcagtggtaa
      421 aatcagtttt agttgatata aaatcaaaat tatttgggta a
//
LOCUS       DAXFWM010000168          455 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000168 DAXFWM010000000
VERSION     DAXFWM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.169"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(112..303)
                     /locus_tag="ACFI8B_002268"
     CDS             complement(112..303)
                     /locus_tag="ACFI8B_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835363.1"
                     /translation="MLPACSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
BASE COUNT          168 a          110 c           63 g          114 t
ORIGIN      
        1 agataaagaa acggcaacac acgccaacag aaaaacatat ttgaacttca tcatattttc
       61 cacataaaag gcagcctgaa aatctttcag gctgcccttg tcaaattatt cctagctttc
      121 ggcttttttg gcaaaccaaa caatccgatt acccgcataa tactttccat ttattgaaat
      181 ccgacaagcc gcgcccaaaa aatgccatgc actgccgatt tccgcagcaa tctttgtacc
      241 gttttcttca aattccaaat attcacccaa taataaactt gaaacagaac acgcgggcag
      301 caagtgccca ccctacgctt attcaaataa tttgattaaa taaagaataa agaaaaggca
      361 tcaggaacaa aattataatc tgccacttga ctcgcaccgc ttttaaagta aggggcatca
      421 aaaccaaaac cgcaaaaaaa aataattttt gcatt
//
LOCUS       DAXFWM010000169          420 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000169 DAXFWM010000000
VERSION     DAXFWM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.170"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            80..>420
                     /locus_tag="ACFI8B_002269"
     CDS             80..>420
                     /locus_tag="ACFI8B_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC3835364.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNGRGSVKNRVCAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSR"
BASE COUNT          122 a           99 c          100 g           99 t
ORIGIN      
        1 cgatgaagca gtgcgacaac atagacaagg gcaaccttga ttcacactgc cataaccgct
       61 tgctgccaag gaaaacaaaa tgaatttgcc tattcaaaaa ttcatgatgc tgtttgcagc
      121 ggcaatatcg ttgctgcaaa tccccattag tcatgcgaac ggtttggatg cccgtttgcg
      181 cgatgatatg caggcaaaac actacgaacc gggtggcaaa taccatctgt tcggtaatgg
      241 tcgcggcagt gttaaaaatc gggtttgcgc cgtccaaaca tttgatgcaa ctgcggtcgg
      301 ccccatactg cctattacac acgaacggac agggtttgaa ggcattatcg gttatgaaac
      361 ccatttttca gggcacggac acgaagtaca cagtccgttc gataatcatg attcaagaag
//
LOCUS       DAXFWM010000170          414 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000170 DAXFWM010000000
VERSION     DAXFWM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.171"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            172..366
                     /locus_tag="ACFI8B_002270"
     CDS             172..366
                     /locus_tag="ACFI8B_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3835365.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPVM"
BASE COUNT          132 a           99 c          102 g           81 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccggtg
      361 atgtgatttg caccacattt gcaccaagtg gtatataaca ttatgaaaga tata
//
LOCUS       DAXFWM010000171          392 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000171 DAXFWM010000000
VERSION     DAXFWM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.172"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..>392
                     /gene="mafB"
                     /locus_tag="ACFI8B_002271"
     CDS             <1..>392
                     /gene="mafB"
                     /locus_tag="ACFI8B_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC3835366.1"
                     /translation="IQPALAADLAQDPFITDNTQRQHYEPGGKYHLFGDPRGSVSDRT
                     GKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHGHEEHAPFDNHAADSASEEKGN
                     VDDGFTVYRLNWEGHEHHPADAYDGPKGG"
BASE COUNT          103 a          141 c           96 g           52 t
ORIGIN      
        1 atacagcccg ccctcgcggc ggacttggcg caagacccgt tcattaccga taacacccaa
       61 cggcagcact acgaacccgg cggcaaatac cacctcttcg gcgacccgcg cggcagcgtt
      121 tccgaccgca ccggcaaaat caacgtcatc caagactata cccaccagat gggcaacctg
      181 ctcatccaac aggcggcaat ccaaggcaat cttggttaca ccgtccgctt ttccggacac
      241 ggacacgaag aacacgcccc cttcgacaac cacgccgccg acagcgcaag cgaagaaaaa
      301 ggcaacgttg acgacggctt taccgtgtac cggctcaact gggaaggaca cgaacatcat
      361 cccgccgatg cctacgacgg cccgaagggc gg
//
LOCUS       DAXFWM010000172          365 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000172 DAXFWM010000000
VERSION     DAXFWM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.denovo.173"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(34..>365)
                     /locus_tag="ACFI8B_002272"
     CDS             complement(34..>365)
                     /locus_tag="ACFI8B_002272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3835367.1"
                     /translation="RFKFNPLYMAGGVYLGGKIISPIYHSSQEFSGEPIIYAETNIIL
                     CPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVRFDKNYDKYKTWQVAIEKAANFL
                     KLGNPYN"
BASE COUNT          124 a           52 c           49 g          140 t
ORIGIN      
        1 gtgtcatgtt cttcactaag agataattta tctttaatta tatggattac cgagttttaa
       61 aaaatttgct gctttttcta tagcaacttg ccaagtttta tatttatcgt aatttttatc
      121 aaaacgtact cccccttgtt tattggataa aaaagtaatt atctgattca tattaaaaat
      181 atttccatta tggaaaactc gttttaaagt taaaaatttt tcagcaggac ataaaattat
      241 atttgtttca gcatagatta ttggttcacc agaaaattct tgtgatgaat gatatatagg
      301 gctaataatt tttccgccta aataaactcc accagccata tagagtggat taaatttaaa
      361 ccggt
//
LOCUS       DAXFWM010000173         1933 bp    DNA     linear   BCT 25-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate SA10
            SAMEA113994288-rid23200403.guided.129, whole genome shotgun
            sequence.
ACCESSION   DAXFWM010000173 DAXFWM010000000
VERSION     DAXFWM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994288
            Sequence Read Archive: ERR11669811
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363979.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363979.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/16/2024 21:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,274
            CDSs (total)                      :: 2,224
            Genes (coding)                    :: 2,028
            CDSs (with protein)               :: 2,028
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 109 of 196
            Pseudo Genes (incomplete)         :: 91 of 196
            Pseudo Genes (internal stop)      :: 46 of 196
            Pseudo Genes (multiple problems)  :: 47 of 196
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994288-rid23200403.guided.129"
                     /isolate="SA10"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            139..669
                     /locus_tag="ACFI8B_002273"
     CDS             139..669
                     /locus_tag="ACFI8B_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC3835368.1"
                     /translation="MSVYLVEAQPAKVIAPTRTAINRIFTDKASGSSTDREGLDLLRM
                     KVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTIL
                     SAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLS
                     IARSTVYKILEDERTS"
     gene            852..1712
                     /locus_tag="ACFI8B_002274"
     CDS             852..1712
                     /locus_tag="ACFI8B_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-135"
                     /protein_id="HFC3835369.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTTPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          530 a          430 c          465 g          508 t
ORIGIN      
        1 acagagcaag taaacttgtt tgcttgttct gcaagggttc gcaataaaaa caaccgctaa
       61 acatttctgc ccagcggttg aaaatttacc tattcaccat tacaatgatc aagcaggaaa
      121 tttttttgat tgccgtaaat gtccgtatat ctagttgagg cacaacccgc caaagtcatt
      181 gccccaacca gaacggcgat aaaccgtata tttaccgata aggcatccgg cagttcaaca
      241 gaccgggaag ggctggattt gctgaggatg aaggtggagg aaggtgatgt cattctggtt
      301 aagaaactcg accgtcttgg ccgcgacact gccgatatga tccaactgat aaaggaattt
      361 gacgctcagg gcgtggcagt ccggttcatt gatgacggga tcagtaccga cggtgatatg
      421 gggcaaatgg tggtcaccat cctgtcggct gtggcacagg ctgaacgccg gaggatccta
      481 gaacgcacga atgagggccg acaggaagca aagctgaaag gaatcaaatt tggccgcagg
      541 cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga agggcactgg tgcaacggaa
      601 attgctcatc agctcagtat tgcccgctcc acggtttata aaattcttga agacgaaagg
      661 acctcgtgat acgcttattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      781 attcaaatat gtatccgctc atgagacaat aaccctggta aatgcttcaa taatattgaa
      841 aaaggaagag tatgagtatt caacattttc gtgtcgccct tattcccttt tttgcggcat
      901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggtg
     1081 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgctgcc aacttacttc
     1261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1381 acaccacgac gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacccct
     1861 atctataaac tcttggcttg gttctaatcc ctctaaacga ttattatcaa tagccgctct
     1921 aaccgctttt tct
//
